LOCUS       DASRMJ010000001         4385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_108427, whole genome shotgun sequence.
ACCESSION   DASRMJ010000001 DASRMJ010000000
VERSION     DASRMJ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108427"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1600
                     /gene="metG"
                     /locus_tag="VFD74_00005"
     CDS             38..1600
                     /gene="metG"
                     /locus_tag="VFD74_00005"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZK47976.1"
                     /translation="MGTFYVTTPIYYVNDVPHIGHAYTTTAADVVARFHRQRGEEVFF
                     LTGTDEHGNKVEQAATARGISPREHADQMVGKFKEVARKVDATNDFFIRTTDPEHEAF
                     VQRFVERCRDRGDIFEGSYSGLYCSACEAYHTEENLVDGLCPDHGTEPTFMEEKNYFF
                     RLSAYQEKLAAHYRAHPDFVRPRHRYNEALSFIEQGLDDISISRATLKWGISVPWDES
                     QVIYVWVDALINYLSALHYAPGDDLLQRFWPASYHMMAQDILKFHGIIWPALLMSAGY
                     ELPEHLFIHGYLKLGGEKMSKTRGNVMDPFPLIERYGPDPFRFYCFREVNFGQDGMVG
                     EESFKARYNSELANDLGNLLSRSVSMIGKYRDGVVPMPADRAPSGSPLAAEAGRVAVT
                     AGDQLEEMDLSGALDTIWELVRRLNRYVEEQAPWKQAKTGDESALDATLYDLAEGLRL
                     LSVLLYPFMPGTVTVMRERLGLAGAAAGGTSGAAVGQGATAPGWDEAVWGRMPEGVTV
                     VQGGSLFPRIEE"
     gene            1619..2398
                     /locus_tag="VFD74_00010"
     CDS             1619..2398
                     /locus_tag="VFD74_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZK47977.1"
                     /translation="MMAPTGYIDTHTHLDHCLVDPNELVAAARLAGVTTLIQSGTDAD
                     SSRLAVELAERFPEVWATVGFHPHEAKDLDAAGAAAIEALVGHPRVLAVGEAGLDYHH
                     DHSPRERQREVFEWHMTVAARAGLPLVVHTREADDHSLDLLAEHGTDLTVVLHCFSMP
                     DRLEELVARGYYLSFAGNVTFPKASALQDAARTAPAERILIETDAPYLTPVPRRGRPN
                     TPAMVTHTYAFVAGLRGVQPAQLVAQVRENAARAFPRLVLG"
     gene            2402..3421
                     /gene="rsmA"
                     /locus_tag="VFD74_00015"
     CDS             2402..3421
                     /gene="rsmA"
                     /locus_tag="VFD74_00015"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZK47978.1"
                     /translation="MAAPSTAGWGDGPRGGSSGGSSGGSSRPPGLRQISLARIAEFGI
                     EPKRRLGQNFLIDDNIIGVILDRLAPTPADVAIEVGAGLGILTQALAAATLSVHAFEI
                     DRSLRPALEATLADAGVRERVHLEYSDIMRVSLEDLRPTPTLCASNLPYSIAAPFLGE
                     ALERLPSIRRYCVMTQREVAERLGSPPGSKAYGAPSVWVQLHARILETRPLSRTIFHP
                     QPNVDSSLITLERAVADPLVTEYPRLLRAVVEGAFQQRRKLALNSLSAVLGVEKVLLA
                     EVLTRAGLAPGVRPEQMVPAAFVEVARALVELGLAGRYNESHDRAGADRLHEEGAKKS
                     GRRSR"
     gene            3402..3890
                     /locus_tag="VFD74_00020"
     CDS             3402..3890
                     /locus_tag="VFD74_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK47979.1"
                     /translation="MAEDRAEGTRRRGTALLVGLMTALLVVALAATLSACSDRPPPDP
                     TMAALGAAVGDAKLDEVDFSALLGSFASESYGAATTSEQADYQLYLAAKAADLGLNRA
                     DTGSVQVDKRVSGTTASFSFTFPVREGLFAVADVGRIDVSLVYADSKSHPWLIESIVL
                     SR"
     gene            3857..>4385
                     /locus_tag="VFD74_00025"
     CDS             3857..>4385
                     /locus_tag="VFD74_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK47980.1"
                     /translation="MAHREHRALAMTVTGRSDGAPGHSPAGSRGGEGAEVRAARGAAA
                     PERRLLVSAPAKVNLSLLVGPPGEDGYHQLGTVFVPLDLADELEMALTARPRHEAPET
                     AEPLTVVCAGVSQDENLVTRALLAVENATRWIFSGHVTVHKNIPTGAGLGGGSSDGAA
                     ALRAGAEMVAEAGGPR"
BASE COUNT          690 a         1473 c         1512 g          710 t
ORIGIN      
        1 aggatcgaac cgctgacgac gggagccacc accgcccatg ggcaccttct acgtgaccac
       61 acccatctac tacgtcaacg acgtgccaca catcgggcac gcctacacga cgacggccgc
      121 cgatgtcgtg gcccgcttcc accggcagcg cggcgaggag gtcttcttcc tcaccggcac
      181 cgacgagcac ggcaacaagg tggagcaggc cgccaccgcg cggggcatct ccccgcgcga
      241 gcacgccgat cagatggtgg gcaagttcaa ggaggtggcg cgcaaggtcg acgccaccaa
      301 cgacttcttc atccgcacca ccgaccccga gcacgaggcc ttcgtccaac ggttcgtgga
      361 acgctgccgc gaccgcgggg acatcttcga ggggtcgtac agcggcctgt actgctcggc
      421 ctgcgaggcc taccacaccg aggagaacct ggtcgacggc ctgtgccccg accacggcac
      481 cgaacccacg ttcatggagg agaagaacta cttcttccgg ctctcggcct accaggagaa
      541 gctcgcggcc cactaccgcg cgcatcccga tttcgttcgg ccgcgtcacc gctacaacga
      601 ggccctctcc ttcatcgagc agggtttgga cgacataagc atctctcgcg ccactctcaa
      661 gtggggcatt tcggtgccgt gggacgagtc tcaggtgata tacgtgtggg tggacgccct
      721 catcaactac ttgagcgccc tgcactacgc tccgggcgac gacctcctcc agcggttctg
      781 gccggcctcc taccacatga tggcccagga catcctcaag ttccacggca tcatctggcc
      841 ggctcttctc atgtccgcgg gctacgaact gcccgaacac ctgttcatcc acggctatct
      901 gaagctgggc ggggagaaga tgtcgaagac ccgcgggaac gtcatggacc cttttccgtt
      961 gatagagcgc tacggacccg acccgttccg gttctactgc ttccgcgagg tgaacttcgg
     1021 gcaggacggc atggtggggg aggagagctt caaagcccgc tacaactcgg agttggcaaa
     1081 cgacctcggc aacctcttga gccgcagcgt gtcgatgatc ggcaagtatc gtgacggcgt
     1141 ggtccccatg cccgcggatc gggccccgag cgggtcgccg cttgcagccg aagccggacg
     1201 ggtggccgtg acggccgggg accagctcga ggagatggac ctgagcggcg cgctcgacac
     1261 catctgggag ctggtgcgtc ggctcaaccg ctacgtggag gagcaggccc cttggaagca
     1321 ggccaagacc ggggatgaat ccgccctcga cgccacgctc tacgatctgg ccgaagggct
     1381 gaggttgctg tctgttctgc tgtacccctt catgccgggc acggtgacag tcatgcggga
     1441 gcgcctgggt ttggccggcg cggcggcggg cggcacaagc ggcgccgccg tgggccaggg
     1501 cgcgaccgcg cccggatggg acgaagccgt ttgggggcgt atgccggagg gtgtcaccgt
     1561 cgtccagggc ggatcgctct tcccgcgcat cgaagagtag gctggtgcgg agcggaggat
     1621 gatggcgccg acaggctaca tcgatacgca cacgcacctc gatcactgcc tcgtggatcc
     1681 gaacgagttg gtggcggcgg cccgacttgc cggcgtcacc actctgatcc agtcgggcac
     1741 cgacgccgat tcgtcacgcc tggccgtgga gttggcggag aggttccccg aggtgtgggc
     1801 gacggtcggc ttccaccccc acgaagccaa ggatctcgac gccgccggcg ccgcggccat
     1861 cgaggcactc gtcggtcatc cgcgcgtgct ggcggtcggc gaggcggggc tcgactacca
     1921 tcacgaccac tcacctcggg agcggcagcg tgaggtcttc gagtggcaca tgacggtggc
     1981 ggcgcgggcg gggctgccgc tcgtggtgca tacgcgggag gccgacgacc attcgctcga
     2041 tcttctggcg gaacacggca ccgatctcac cgtcgtcctg cactgtttct ccatgcccga
     2101 tcgcttggag gagctcgtag cgcgcggata ctacctgagc ttcgccggca acgtgacctt
     2161 tcccaaagct tccgccctcc aggacgcggc gcgcacggcg cccgcggaga ggatcctgat
     2221 cgagaccgac gctccctatc tgacccctgt gccgcgccgc gggcgcccca acacgccggc
     2281 gatggtcact cacacctacg ccttcgtggc ggggttgcgc ggtgtgcagc cggcacagct
     2341 tgtggcccag gtgcgggaga acgcagcgcg cgcgttcccg cggctggtcc tgggataagg
     2401 ggtggcggcg ccgtccacag caggctgggg cgacggtcca aggggcgggt cgagcggcgg
     2461 gtccagcggc gggtccagca ggccccccgg actgaggcag atctccctgg cccggatcgc
     2521 ggagttcggc atcgagccca aacgccgtct gggccagaac tttctcatcg acgacaacat
     2581 catcggggtc atcctcgacc ggctcgcgcc gaccccggcg gacgtcgcca tcgaagtggg
     2641 cgcggggctg ggtatcctga cccaggcgct ggccgcggcg accttgtcgg tgcacgcctt
     2701 cgagatcgat cggagtctga ggcccgcact cgaagccact ctggccgacg ccggggtccg
     2761 ggagcgtgtg cacctggagt attccgacat catgcgcgtc tcgttggagg atctgaggcc
     2821 cacgccaacc ttgtgcgcga gtaacctgcc ctactcgata gccgcaccgt tcctgggcga
     2881 ggcgttggaa cgactcccct ccatccgccg ctactgcgtg atgacacagc gggaggtggc
     2941 ggagcggctc ggctctccgc cgggcagcaa agcctacggc gcgccctccg tgtgggtgca
     3001 gttgcacgcg cggatcctgg agacacgccc cctatctcgc accatattcc accctcagcc
     3061 gaacgtggac tcgagcctga tcacgctgga gcgggccgtc gccgaccctc tggtgaccga
     3121 gtatccaagg ttgctgcggg ccgtcgtgga aggggcgttc cagcagcggc ggaagctcgc
     3181 gctcaattcg ctgtcggcgg tgctgggcgt ggagaaggtc ctgctcgcgg aggtgctgac
     3241 ccgggccggc ctggcccccg gtgtgcggcc tgagcagatg gtgccggcgg cgttcgtcga
     3301 ggtggcgcgt gccctggtgg agctggggtt ggccggtcgc tacaatgagt cgcacgatcg
     3361 ggccggcgcg gaccggcttc acgaagaagg ggcgaagaag agtggcagaa gatcgcgctg
     3421 aaggcacgcg ccggcgcgga acggcgctcc tggtcgggtt gatgacggct ctcctcgtgg
     3481 tggccctcgc ggccaccctg tcggcgtgtt ccgaccggcc accgcccgac ccgaccatgg
     3541 ccgctctggg cgccgccgtg ggtgacgcca agctggatga ggtcgacttc tccgccctgc
     3601 ttggctcctt cgcgtccgag tcgtatggag ccgcgaccac gtccgagcag gccgactacc
     3661 aactctacct ggctgccaag gcggccgacc tgggcttgaa cagagccgac accggcagcg
     3721 tgcaggtgga caagagagtc tcgggcacga cggcgtcgtt ctcgttcaca ttccccgtcc
     3781 gggaggggct gttcgcggtg gcagatgtgg gtcgcatcga cgtgtccctc gtctacgccg
     3841 actccaagtc gcatccatgg ctcatcgaga gcatcgtgct ctcgcgatga cggtgaccgg
     3901 tcgttccgac ggcgcgccgg gccactcccc tgcgggatcg cggggtgggg agggggccga
     3961 ggttcgggcc gcgcgcggcg ctgcggcccc agagcggcgg ctgctcgtct cggcgccggc
     4021 caaggtcaac ctctctctgt tggtcggccc gcccggtgag gacggctacc accagctggg
     4081 cactgtgttc gtccccctag acttggccga cgagctggag atggccctga ctgctcgccc
     4141 ccggcatgaa gctccggaga ccgctgagcc cctgacggtg gtgtgcgcgg gtgtctctca
     4201 ggacgagaac ctggtgaccc gggcgctgct ggccgttgag aacgcgaccc gctggatctt
     4261 cagcggccac gtcaccgtgc acaagaacat ccctacgggc gccggtttgg gtggcggcag
     4321 ctcggacggc gcggcggcgc tccgcgccgg cgccgagatg gtggcggaag cgggtggccc
     4381 gaggt
//
LOCUS       DASRMJ010000002         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_108476, whole genome shotgun sequence.
ACCESSION   DASRMJ010000002 DASRMJ010000000
VERSION     DASRMJ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108476"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..2211
                     /locus_tag="VFD74_00030"
     CDS             226..2211
                     /locus_tag="VFD74_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK47981.1"
                     /translation="MARQIFEWDYSEYPGAKKYPHVFSPIQIGGLTIPNRIKYAATED
                     NLNSHDGYVTDEDVAYIRARAKGVAGGICTMQGVYMDERRYGQGYVGQAAAWDDKFVP
                     GLKRLADAIHDERAVANYQLMHCGRVGGVETPCCEGPSYLPQRLRLFKPVHEMSAEEV
                     QISIQQHVDAARRGIEAGFDMMEISGIVGYLISNFLSAYTNRRTDEYGGDIYGRCKFM
                     TDIIKGVRGAIGRDVPLIIRICAWEQLDDVGGNTQEESMIVYQEAQKAGVDSISVTVG
                     WQESIVPVISRDIDFGTWLWVPENAKKHVDVPLSMAYRLFHADMADKAIADGKIDYWE
                     NCRPQIADPLMPLKYLEGREEDIRWCVACNVCLARLFRDAPMTCYINPLCAHENDPEY
                     YPQPAKTKKTVFVVGGGPAGLECAAGAAERGHEVHVYEKGEKLGGTIIDAGLAPYNDD
                     ELYGIIEFHKAQCDQRGVEFHLGEEVTADLLEEELPDTVVLAIGSTVRKPTFFGGDRD
                     NVITLKEALYEEKPVGDNVVIWGNKKPAIGTALALAKRGKKVTLVGKERRVGKDINPS
                     FRWRYNIFLRQNGVVSYNDCDVEQVGDGQAILITYDGHRVPVKCDTVVLGERDPNPAL
                     KQAVQDNSIELYVIGDAVVPRGLSGAVHDGYKTGLRI"
     gene            2242..>2615
                     /locus_tag="VFD74_00035"
     CDS             2242..>2615
                     /locus_tag="VFD74_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK47982.1"
                     /translation="MAEVQEKPAEGVKQPKPFNAEAEIAKLWAKCQAAQEEIRRAFYA
                     WRAAAVEMAGDGADETVIALKAWESRGKDVAKTYFPRINLEKGSLVEQIGKLYVNSWR
                     NQGAVVKLQKSENPDEVFIVWER"
BASE COUNT          533 a          804 c          877 g          401 t
ORIGIN      
        1 tctcgagata gcgatggccc gacagggcgg tccggccgtg cgggaatgac ggcccatcgc
       61 gggtgctcgg ggcatcggag cctggcgggg cggggacgca cccgtcacgc gagagggcga
      121 aaggaggtgg tctggtacgc cccctgaagg ggttagcccc ccgcaggggg agcacgcgac
      181 cgtaatgcac atgagggatg tagcctgagg gagaagaacg gagcgatggc acgacagatc
      241 tttgagtggg actattcgga gtatccgggg gccaagaaat acccccacgt attctcgcct
      301 atccagatcg gcggtctgac gatccccaac cggatcaagt acgccgccac tgaggacaac
      361 ctaaacagcc atgacggcta cgtcaccgac gaggacgtgg cctacatccg cgcccgcgcc
      421 aaaggcgtgg cgggcggcat ctgcaccatg cagggcgtct acatggacga gcgccgctac
      481 ggccaagggt atgtgggtca ggcggctgct tgggacgaca agttcgtccc cggcctgaag
      541 cggctggcag acgcgatcca cgacgagcgt gcggtcgcca actatcagct gatgcactgc
      601 gggcgcgtcg gtggtgtcga gaccccgtgt tgtgagggtc cctcctacct gccgcagcgg
      661 ctgcgtctct tcaagcccgt ccacgagatg agcgccgaag aggtgcagat ctcgatccaa
      721 cagcacgtcg acgccgcccg ccgtggcatc gaggccggct tcgacatgat ggagatctcc
      781 gggatcgtgg gctacctgat ctcgaacttc ctctccgctt acaccaaccg gcgcaccgac
      841 gagtacggcg gcgacatcta cggccgctgc aagttcatga ccgacatcat caagggtgtg
      901 cgcggcgcca tcggtcgcga cgtcccgctg atcatccgca tctgcgcctg ggaacagctc
      961 gacgatgtag gcggcaacac ccaggaagag tcgatgatcg tgtaccagga agcgcagaag
     1021 gccggggtcg actcgatcag cgtgaccgtc ggctggcagg agtcgatcgt tccggtgatc
     1081 agccgcgaca tcgactttgg cacctggctc tgggtgcccg agaacgccaa gaaacacgtg
     1141 gacgtgccgc tctcaatggc ttaccgcctc ttccacgccg acatggccga caaggccatc
     1201 gccgatggca agatcgacta ctgggagaac tgccgtcccc agatcgcgga tccgctcatg
     1261 cctctcaagt acctcgaggg tcgcgaagag gacatccgct ggtgcgtggc ctgcaacgtc
     1321 tgcctggccc gtctgttccg tgacgcgccc atgacctgct acatcaaccc gctctgcgcc
     1381 cacgagaacg acccggagta ctatccgcag ccggcgaaga ccaagaagac ggtcttcgtg
     1441 gtgggcggcg gtccggccgg cctcgagtgc gcggccggcg cggcggagcg cggccatgag
     1501 gtgcacgtgt acgagaaggg cgagaagctc ggcggcacga tcatcgacgc cggcctggcc
     1561 ccctacaacg acgatgagct ctacggcatc atcgagttcc acaaggctca gtgtgatcag
     1621 cgaggggtgg agttccacct tggcgaagaa gtcaccgccg acctactcga ggaggagcta
     1681 cccgacaccg tggtgctggc gatcggctcg accgtgcgta agcccacctt cttcggcggc
     1741 gaccgcgata acgtcatcac gttgaaggaa gcgctctacg aagagaagcc cgtcggcgac
     1801 aacgtggtta tctggggcaa caagaagccc gccatcggca ccgccttggc gctcgcgaag
     1861 cgcggtaaga aggtcactct ggtgggcaag gagcgtaggg tgggcaagga catcaacccg
     1921 tccttccgtt ggcgctacaa catcttcctc cgccagaacg gtgtggtcag ctacaacgac
     1981 tgcgacgtcg agcaggttgg cgacgggcag gcgatcctga tcacctacga cggccacagg
     2041 gtgccggtga aatgcgacac ggtcgtgctc ggcgagcgcg atcccaaccc ggctctcaag
     2101 caggctgtgc aggacaactc gatagagctg tacgtcatcg gcgacgccgt cgtgcctcgg
     2161 ggcctctcgg gcgccgtgca cgatggctac aagaccggcc tgcgcatctg acggccgaca
     2221 ggaagaggaa ggagaaccac agtggcagag gtgcaggaaa agcccgccga gggcgtgaag
     2281 caaccgaaac cgttcaatgc cgaggccgag atcgcgaagc tgtgggccaa gtgccaagca
     2341 gctcaggagg agatccggcg cgcgttctat gcgtggcgtg ccgccgccgt ggagatggcc
     2401 ggcgacggcg ccgacgagac cgtcatcgcc ctcaaggcgt gggagtctcg aggcaaggac
     2461 gtcgccaaga cgtacttccc ccgtatcaac ctggagaagg gcagcttggt ggagcagatc
     2521 ggaaagctgt acgtgaacag ctggcgcaac cagggcgcgg tcgtgaagct gcagaagagc
     2581 gagaaccccg atgaggtgtt catcgtgtgg gagcg
//
LOCUS       DASRMJ010000003         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_122, whole genome shotgun sequence.
ACCESSION   DASRMJ010000003 DASRMJ010000000
VERSION     DASRMJ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1598)
                     /locus_tag="VFD74_00040"
     CDS             complement(186..1598)
                     /locus_tag="VFD74_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZK47983.1"
                     /translation="MSDGDPRRRRSASEEDCELVRLLSIALRTAELHESSNAVAETAV
                     SDLYRALRGRLDAVGEVAISTRARYLFVNGERVRLNASDYLHLRYLGQMCDVWTIGGV
                     ILLPGLAQSELSSLVFVLSRDRRRSAEILRQRLVRERAVHVEIMPPVDDDEFGGEDLP
                     ERTYGACVDVMDELRSSISQNRQISTRRARRVTQAVVDQILADEFGLLALTTIKEFDD
                     NLFTHSANVAILSVALGQRIGLTRSQLGELCLAAFLHDLGKIAVSREILDKPGMLNRE
                     ELSEIMQHPIHSVHILLNQGHLSQSTLRAIIGGFEHHINYDLSGYPTLNQKSRVTLFG
                     RIITVVDRYDALTTPRVYRKMNLTPHEGIAYLLENSGTFFDPILVKVFVGILGLYPPG
                     TVVGLSDDTVAVVEQPPAPGMPADRPKVRVLRGDQAGHRLDLSLKDDGGEYERTVSGV
                     HNPGNRGQLPAIDAEMAARL"
     gene            complement(1648..>2055)
                     /locus_tag="VFD74_00045"
     CDS             complement(1648..>2055)
                     /locus_tag="VFD74_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK47984.1"
                     /translation="VAACLDDPAPSVRVLAARSLPRTTPHEAAATLLAQVTSRDFHRR
                     DGAEIDAFFEALAEVADDTTIRGLNYLWSDRPTLRSRPTAIRLGALKVAGKTASPAAR
                     EMLARATRSGDAEVRRQAKRILEEAERTAGGRR"
BASE COUNT          326 a          655 c          709 g          365 t
ORIGIN      
        1 cctcgggggg ggagaaaggc cggcatgggg ggcgatctaa ggtcgttccg cataatgttc
       61 agcggcgaca aacccattgt agcgcgccga tcggtgcgtg taaaggggtg aatggcgccg
      121 aatgacgtaa tgagcggtac aggaggaggg ttgaacggac agcggcgggc tcgaacgatg
      181 gtccgtcaaa gtcttgcggc catctccgcg tcgatcgccg ggagttgtcc gcggttccca
      241 ggattgtgga cgccgctcac ggtccgctcg tattcgccgc cgtcgtcctt cagggagagg
      301 tccagccggt gccctgcctg gtctcccctc aacacccgga ccttcggcct gtccgcgggc
      361 atgcctggtg cgggcggctg ttccaccacc gcgacggtgt cgtcgctcaa gccgaccacc
      421 gttcccggcg ggtagaggcc caggatcccc acgaacacct tcacgaggat cgggtcgaag
      481 aatgtccccg agttctccag aagataggcg atgccctcat ggggggtcag gttcatcttc
      541 cggtagacgc gcggggtcgt caacgcgtca tagcgatcca ccacggtgat gatgcgaccg
      601 aagagcgtga cgcgcgactt ctggttgaga gtcggatacc cggagagatc gtagttgatg
      661 tggtgctcga acccgccgat gatcgcccgg agcgtcgact ggctgaggtg gccttggttg
      721 aggaggatgt ggaccgagtg gatgggatgc tgcatgatct cggagagttc ttcgcggttg
      781 agcatgcccg gcttgtcgag gatctcgcgc gacacagcga tcttgcccag atcgtgcagg
      841 aacgccgcta gacacagttc gccgagctgg ctcctggtga ggccgatcct ctgaccgaga
      901 gccacggaga ggatggccac gttcgcggag tgggtgaaga ggttgtcgtc gaactccttg
      961 atggtggtca gagcgagtag cccgaactcg tccgccagta tctggtccac cactgcctga
     1021 gtcaccctgc gcgcgcgacg cgtgctgatc tggcggttct gcgagatcga ggagcgcaac
     1081 tcgtccatga cgtcgacgca ggctccatat gttcgctcag gcaggtcttc gccgccgaac
     1141 tcgtcatcgt cgaccggcgg catgatctcc acgtgcacgg cccgctcccg caccaaccgt
     1201 tggcggagta tctccgcgct cctgcgccgg tctctcgaga gcacgaacac gaggctcgag
     1261 agttcgctct gggcaaggcc ggggaggagg atcacgccgc cgatggtcca cacgtcacac
     1321 atctgtccga ggtagcggag gtgcaggtag tccgaggcgt tcagccgcac ccgttccccg
     1381 ttcacgaaca ggtaccgcgc ccgcgtggat atcgcgacct cgcccactgc gtccagccgc
     1441 ccgcgcagcg ctctgtagag gtccgacaca gcggtctccg cgaccgcgtt gctggattcg
     1501 tggagttcgg ccgtccgaag cgcgatggag agcaagcgca ccagttcgca gtcctcctcg
     1561 ctcgctgaac ggcggcgccg ggggtccccg tcgctcatgg cgtcctccca gtggctcggc
     1621 gtatcgtgag acgtggcatc gcgcccgtca cctccggccg ccagcggtgc gttccgcttc
     1681 ttcgaggatg cgcttcgcct ggcgccgcac ctccgcgtct ccggaccggg tcgctcgggc
     1741 gagcatctct cgtgcggccg gcgacgccgt cttcccggcg accttcagcg ccccgagccg
     1801 gatcgcggtg gggcggcttc gcaacgtggg gcgatccgac cagaggtagt tgagaccgcg
     1861 tatcgtcgtg tcgtcggcga cttccgccag ggcctcgaag aaggcgtcga tctccgcgcc
     1921 gtcccggcga tggaagtccc gtgacgtgac ctgggcgagg agggtggccg ccgcctcgtg
     1981 gggcgtcgtc cgggggaggc tccgtgccgc cagcacgcgc acggatggag cggggtcgtc
     2041 caagcacgcg gcgac
//
LOCUS       DASRMJ010000004         6926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_72550, whole genome shotgun sequence.
ACCESSION   DASRMJ010000004 DASRMJ010000000
VERSION     DASRMJ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72550"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /gene="ffs"
                     /locus_tag="VFD74_00050"
     ncRNA           complement(<1..123)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFD74_00050"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(304..393)
                     /locus_tag="VFD74_00055"
     tRNA            complement(304..393)
                     /locus_tag="VFD74_00055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(356..358),aa:Ser,seq:gga)
     gene            complement(582..1334)
                     /locus_tag="VFD74_00060"
     CDS             complement(582..1334)
                     /locus_tag="VFD74_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK47985.1"
                     /translation="MNGGRSIAPVKGSVRWLLVALLLWEGLELLRNTGPASFSGRPIG
                     LFELLLVTLAPVLIALGFVRLFIAVIGAGYGSLNTYTLTSSPWAWVFWAALALTLAGH
                     GEHAGAHLLNQAVPEVVRHGDFGVTARFFDETLGHWLLSVGLFAMSSVILVLGQGAAR
                     RVVAGEQMLLIVGSVVTYGGGILYIGVEGQMLTPAIVAAVGLAAVGLRHLSMWDVDHD
                     PVSLLMLPGAAAGALVLVVWGLVVGGQPTWPW"
     gene            complement(1436..2632)
                     /locus_tag="VFD74_00065"
     CDS             complement(1436..2632)
                     /locus_tag="VFD74_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZK47986.1"
                     /translation="MEAPLTAPQVAVVRCEGYRAEDVFGAVGRGLDLLGGAERFARPG
                     ERLLLKPNLLVPSTPDDAVTTHPTVFAAVARHLAAAGATLSYGDSPAFGPTRTAARRA
                     RIAQAADELGIARADFHEARTVSFPEGRLIKQFDIAASVLDADALISLPKMKTHALTR
                     ITGAIKNQFGCIPGVRKGAFHARVPDAEHFTQMLVDLNRLLRPRLFVMDGIVAMEGNG
                     PRGGDPRAMNVLLISDDPVAMDATYCRMVGLDPALVGTIAWGEAWGLGRAHGVELLGD
                     PLDSFLAPDFKVNRRPGSTTGGAGWHVPILRDRLVPRPVIDHSICTSCGTCVKVCPVE
                     PKAVEFRKDSRTGSGQGPSAAGGARSAPPVHDYSRCIRCYCCQEMCPDHAIFVKMPLL
                     GRLVHR"
     gene            complement(2800..5187)
                     /locus_tag="VFD74_00070"
     CDS             complement(2800..5187)
                     /locus_tag="VFD74_00070"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZK47987.1"
                     /translation="MLFATLSLAFAPAALAAEGGEANLVLPDLGSVSFIGISGQLLLT
                     LGLFVCGLGLLFGLVIYVQLKNMPVHSSMREISELIWETCKTYLFQQGKFLLILWLFI
                     GVIMAFYFGVLQNFSLLKVLVILIFSLLGIGGSYGVAWFGIRINTFANSRTAFASLRG
                     KPYNIYNIPLKAGMSIGMLLISVELLVMLCVLLFVPRDYAGATFIGFAIGESLGASAL
                     RIAGGIFTKIADIGSDLMKIVFNIKEDDARNPGVIADCTGDNAGDSVGPTADGFETYG
                     VTGVALISFILLAVTETQVQVSLLVWIFAMRVMMIVASGGSYLVNAAIARARYAHADK
                     MSFEAPLTTLVWLTSIVSVAFTYAASYLLIADLGDGTLWWKLSTVITCGTLAGAIIPE
                     LVKVFTSVKSAHVREVVTSSREGGPSLNILSGLVAGNFSAYWLGLTIVSLMGIAYGVS
                     TLGLSDLMIAPAVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSLIEEVPDI
                     AAEIERDFGFKPDFSVAKHNLEENDGAGNTFKATAKPVLIGTAVVGATTMIFSIIVLL
                     TDGLTTGIENLSLLHPPFLLGLISGGAMIYWFTGASIQAVTTGSYRAVEFIKANIRLD
                     DTVVKASVSDSKKVVEICTQYAQKGMFNIFLAVFFATLAFAFLEPFFFIGYLISIAIF
                     GLYQAIFMANAGGAWDNAKKIVEVDLKEKGTDLHSATVVGDTVGDPFKDTSSVAMNPV
                     IKFTTLFGLLAVELAVSMAHEQGRGVTLALAGAFFLVSVVFVWRSFYGMRIPMVD"
     gene            5660..6145
                     /locus_tag="VFD74_00075"
     CDS             5660..6145
                     /locus_tag="VFD74_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK47988.1"
                     /translation="MSVMTEVGRDLESSAGTAATSPSPPGPAVDGGHRGAGPAVAWVM
                     VASWVLAAHFLRSFDLPFVLFFLLLPMLLAVRRAWAARIAQVALLFGVFEWVATTARL
                     LAERRATGEPAGRMVLIMAAVIVFTAAAAAALESKGARKRFGLRTLGFFSAGPPSQPG
                     E"
     gene            6436..>6926
                     /locus_tag="VFD74_00080"
     CDS             6436..>6926
                     /locus_tag="VFD74_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZK47989.1"
                     /translation="MAALDRFFHLTERGTNVRTEVIAGFTTFMTMAYIIFVNPGILSA
                     AGVPFAAAATATALGAALMTLSMALIANRPFALASGMGLNAVVTFSVIGFQQANVPWQ
                     VGMSVIFAEGIIILILVLTGLREAVMNAIPVNLKRAIGVGIGLFITTIGMNEGGFIRP
                     APIT"
BASE COUNT         1216 a         2179 c         2401 g         1130 t
ORIGIN      
        1 gacccggcct tcaacgccac ttgatggcgg catgacctca cacgtccgct cctcgggtgg
       61 gaattcagcc ccgctgtagc ggattgcggg tacagggcac cgctatctcc ccacctagca
      121 cggtcgcgcc gaagtatacc agcctacgcg gccgcccggc acaacacgct gggggtcgac
      181 caccgactgt gtgcgggagg agcggcgcgc gccgccgggc ccgaagagac atgagtggcg
      241 cggccgccag gcgatgcggc gaccgtcgcg ccgtgaagtg acccgctcag gggagaaagg
      301 aagcggagag ggagggattc gaaccctcga gacaggttgc cccgcctaca cgatttccag
      361 tcgtgctcct tcggccagct cggacacctc tccacgtcac gggaggccct ggtgcggcca
      421 ccttgccgcg cgcgggaccg gcctcgaaaa cagacgggat tatagcagaa gcgcgcctgc
      481 ctccgcctgt catcgccctc gcaggggtgc gccgggagtc gaggtcggcg ggccgggacc
      541 tcggcgttcg gcacccggcg actcccgacc ttggcccgtc cctaccaggg ccaggtgggt
      601 tgacctccta ccacaagccc ccaaacgacg aggaccagcg cgcccgccgc cgcaccggga
      661 agcatcagca ggctcaccgg gtcgtgatcg acgtcccaca tcgagaggtg acggaggccc
      721 accgccgcca accccaccgc cgcgacgatg gccggcgtga gcatctgacc ttcgacgccg
      781 atgtagagga tgccgcctcc gtaggtgacc accgacccca cgatgaggag catctgctct
      841 cctgcaacga ctctccgggc ggctccttgg ccgagcacca ggatcacgga actcatcgcg
      901 aacaggccca cggacaggag ccagtggccg agggtctcgt cgaagaaccg ggccgtgacg
      961 ccgaaatcgc cgtgacgcac gacttccggc accgcctggt tgagcaggtg cgcacccgcg
     1021 tgttcgccgt ggccggcaag cgtcagcgcc agcgccgccc aaaagaccca agcccaagga
     1081 ctcgaggtga gcgtgtaggt gttcaaggac ccgtagccgg ctccgatcac cgctatgaac
     1141 aggcgcacga acccgagggc gatgagcacg ggcgccagcg tcacaaggag tagttcgaag
     1201 agtccgatag gtctaccgga gaaggaggcc gggccggtgt tccggagcag ctccagaccc
     1261 tcccacagca ggagtgccac gaggagccag cgcacgctgc ccttgacggg agcgatcgag
     1321 cgtccgccgt tcatgccgcc ggtgccgagt gcgtcggagg tggagcgtgc cgggtgtcgg
     1381 acgagcggtt cacgaagtct tgattataac cggctcttct tgcgccgagc acctctcatc
     1441 gatgcacgag acgtccaagc aggggcatct tcacgaagat ggcgtggtcg ggacacatct
     1501 cctggcagca gtagcagcgg atacagcggg agtagtcgtg gacgggcggc gcggatcttg
     1561 cgccgccggc cgcagagggg ccctgccccg agccggtgcg gctatccttc cggaactcca
     1621 ctgccttggg ctccacgggg cagactttca cgcaggtccc gcacgacgtg cagatcgaat
     1681 ggtcgatcac cggtcgcggc actaggcggt ctctgagtat cgggacgtgc cacccggcgc
     1741 cgccggtcgt ggagccgggg cgtcggttca ccttgaagtc gggggcgagg aacgaatcga
     1801 gcgggtcgcc caacaattcg acgccgtgag cgcggccgag tccccaggcc tcgccccagg
     1861 cgatcgttcc cacgagcgcg gggtcgaggc ctaccatgcg gcaataggtg gcgtccatgg
     1921 ccacggggtc gtcagagatg agcaggacgt tcatggcgcg cgggtctcct ccccgcgggc
     1981 cgttgccctc catggccacg atcccgtcca tgacgaacaa gcggggccga agcaggcggt
     2041 tcaggtcgac gagcatctgc gtgaagtgct cggcgtcggg gacgcgggcg tgaaaggcgc
     2101 ccttgcgtac gccggggatg catccgaatt ggttcttgat cgcacccgtg atccgcgtga
     2161 gggcgtgggt cttcatcttg gggagtgaga tcagggcgtc ggcgtcgagg accgaggcgg
     2221 cgatgtcgaa ctgcttgatg aggcgcccct cgggaaagga gacggtccgt gcctcgtgga
     2281 agtcggcgcg tgcaataccc agctcgtcgg ccgcctgggc tatcctcgcc ctgcgggcgg
     2341 ctgttcgcgt gggtccgaat gcgggcgagt cgccgtagct gagtgtcgcg ccggcggcgg
     2401 ccaggtggcg cgccaccgcc gcgaagacag tcgggtgcgt ggtcaccgcg tcgtcggggg
     2461 tgcttggcac cagcaggttg ggcttgagca ggagacgttc ccccggccgc gcgaaccgct
     2521 ccgcgccgcc gaggaggtcg aggcctcgac cgacggcgcc gaacacgtcc tcagctcggt
     2581 agccctcgca ccggaccacg gcgacctgag gtgctgtcaa tggtgcctcc atgggttcct
     2641 accttactgc ccccaggcct cggatgccag aacgcgtggg gctgggcgac tgtcaaggga
     2701 tggggccggg gcgactttcg tcgccccggc cccgagaagt cacatgctgc gtcgccgcgg
     2761 tctccggaga ccgggctccg ctctggcgag ggatccctgc tagtcgacca tggggatgcg
     2821 catgccatag aaggagcgcc acacgaacac cacggagacg aggaagaagg cgcctgccag
     2881 cgcgagcgtc acgccccggc cctgctcgtg ggccatgctc accgccaatt ccacggccag
     2941 caggccgaac agggtggtga acttgatcac cgggttcatg gccaccgagg aggtgtcctt
     3001 gaaggggtca ccgacggtat cgcccaccac agtcgcggag tgcaggtcgg tgcccttctc
     3061 cttgagatcc acctcgacga tcttcttggc gttgtcccac gcgccgccgg cgttggccat
     3121 gaagatggcc tggtacagcc cgaagatcgc gatggagatg aggtagccga tgaagaagaa
     3181 cggctccagg aacgcgaagg ccagggttgc gaagaacacg gccaggaaga tgttgaacat
     3241 ccccttctgc gcgtactgcg tgcagatctc caccaccttc ttgctgtcac tcaccgaggc
     3301 tttgaccacg gtgtcgtcga gcctgatgtt cgccttgatg aactccaccg ctcggtacga
     3361 gccggtggtg acggcctgga tggaggcgcc ggtgaaccag tagatcatgg cgccgccgct
     3421 gatcagcccg agcaggaacg gggggtgcag cagagagagg ttctcgatgc cggtggtgag
     3481 gccgtcggtg aggagcacga tgatcgagaa gatcatggtc gtggcgccca ccacagccgt
     3541 gccgatgagc acgggcttgg ccgtcgcctt gaaggtgttc ccggctccgt cgttctcttc
     3601 gaggttgtgc ttggccaccg agaagtcggg cttgaagccg aagtcacgct cgatctccgc
     3661 tgctatgtcg ggcacctctt cgatcagcga gagctcgtag accgattggg cgttgtcggt
     3721 gaccgggccg taggagtcca cggcgatggt caccggaccc atgcccagga agccgaaggc
     3781 cacgaggccg aaggcgaaga cggccggcgc gatcatgagg tccgagaggc cgagggtgct
     3841 cactccgtag gcgatgccca tgaggctcac gatggtgagg cccagccagt aggcgctgaa
     3901 gttgccggcc accagcccgg agaggatgtt cagagacggt ccgccttcac gcgacgaggt
     3961 gaccacctcg cgcacgtggg ccgatttgac cgatgtgaac actttgacca gctccgggat
     4021 tatggcgccc gcaagcgtgc cgcaggtgat caccgtagag agcttccacc acagggtgcc
     4081 gtcaccgaga tcggcgatga ggaggtagct ggccgcgtag gtgaaggcca ccgacacgat
     4141 cgaggtgagc cacaccagtg tggtcagcgg tgcctcgaag ctcatcttgt cggcgtgtgc
     4201 gtagcgggcc cgggcgatgg cggcgttgac cagataggac ccgccgctgg ccacgatcat
     4261 catcacgcgc atggcgaaga tccacaccag aagcgacacc tgcacttggg tctcggttac
     4321 cgcgagcagg atgaacgaga tcagggccac gccggtcacg ccgtaggtct cgaagccgtc
     4381 ggcggtgggg cccacggagt cgccggcgtt gtcgccggtg cagtcggcga tgacgcccgg
     4441 gttgcgcgcg tcgtcttcct tgatgttgaa gacgatcttc atgaggtcgg agccgatgtc
     4501 ggcgatcttg gtgaagatgc cgcccgcaat gcggagggcg gaggcgccca aagactcgcc
     4561 gatggcgaag ccgatgaagg tggcgccggc gtagtcgcgc ggcacaaaca ggagcacgca
     4621 gagcatcacg aggagctcga cgctgatgag gagcatcccg atactcatgc ccgccttgag
     4681 cgggatgttg tagatgttgt acggcttgcc gcgcaggctg gcgaaggccg tgcgcgagtt
     4741 ggcgaaggtg ttgatgcgga tgccgaacca ggccacgccg tagctgccgc cgatgcccag
     4801 caggctgaag atgaggatca ccagcacctt cagtagcgag aagttctgca gcacgccgaa
     4861 gtagaaggcc atgatgacgc cgatgaacag ccagaggatc agcaggaact tgccttgctg
     4921 gaagaggtag gtcttgcatg tctcccagat gagctcggag atctcacgca tggaggagtg
     4981 caccggcatg ttcttgagct gcacgtagat gaccaggccg aagagcaggc ccagaccgca
     5041 cacgaacagg cccagggtca ggagaagttg tccgctgatg ccgatgaagc ttacgctgcc
     5101 caggtcgggc agcaccaggt tggcctcgcc gccttcggcc gccagtgccg ccggcgcgaa
     5161 cgccagcgaa agcgtcgcga acagcacgaa aaccgctgtc gccaagaacg aacgggcccc
     5221 ggggcggcgt tcccgagacc gatagatcgc gtccactcac atcctcctag gtcgacacgc
     5281 cgggttcatg tttaagcgtg cttaaagata cttgtgagta tgttacacgc ggtcgagcgc
     5341 gactgtcaat cggtgggcgg cacaagatcg aggtcgcgtg gtatcaggat ggccataggc
     5401 ggcccgaccg tcttgagaag caggccgagg tcggtgccgc gggagcgcgc ctggttcgag
     5461 acgcaggctt cggcgaagtg gccgaaacgg gcatccgcgc ttggcgctct tcgtgcggag
     5521 atgtatcggg cgtactcgga gggggctgcg gggtagatgc gacagggagt cggcgtcgca
     5581 cgtcatccca agtcggtgcg ccggggcggt ggaccggcgg tgtcggtaga gatcgagtgg
     5641 tcacgaaggt ggaggtagca tgtcagtgat gacggaggta gggagggatc tggagtcgag
     5701 cgcggggaca gccgctactt cgccgagtcc gcctgggccg gcggtggacg gcggccaccg
     5761 cggggcgggc ccggcggtgg cgtgggtgat ggtagcctcg tgggtgcttg ccgcccactt
     5821 ccttcgctcc ttcgaccttc cgttcgtgtt gttcttcctg ctcctgccca tgctcctggc
     5881 ggtgcggcgg gcctgggccg cgcgtatcgc gcaggtggcg ttgctgttcg gggtgttcga
     5941 gtgggttgcg accacggctc gacttctcgc cgagcgtcgc gccacgggag agcccgccgg
     6001 gcgcatggtg ctcatcatgg ccgcagttat cgtcttcacc gcggcggctg ccgctgcatt
     6061 ggagtcgaag ggagcgcgca agcggttcgg gcttcggacc ctcggcttct tctcggcagg
     6121 gccgccgtca cagcctgggg agtagctgga tcgtacgcac gccgtccgga tccaacgccc
     6181 ccggaacccg gtggctgtcg gaatcctgcg gccgccgccc cacgattgac cgggcgccct
     6241 gcgacgggcg gcgaaggcga gctgcgaacg gcgtaggggg acagaagcgg gcttcactca
     6301 cgaatagctc ttccgtccgt tgccggtacg gtttagtatc gccctcaaca tgtggccgtt
     6361 ccggatccgg gcgcggagtc gccgcccgat cccgcggccg cgaaacgagc gccggaaacg
     6421 gcagggaggt ctacattggc agcactcgac cgcttcttcc atctcaccga gagggggacc
     6481 aacgtcagga ccgaggtgat cgccgggttc accaccttca tgacgatggc ctacatcatc
     6541 ttcgtgaacc cagggatcct ctcggctgcg ggcgttcctt tcgccgccgc ggcgacggcc
     6601 accgccctcg gcgccgcact catgaccctc tccatggcct tgatcgcgaa ccgtccgttc
     6661 gccttggcct cgggtatggg cttgaacgcg gtggtcacgt tctcggtcat cggtttccag
     6721 caggcaaacg tgccctggca ggtgggcatg tcggtgatct tcgctgaagg catcatcatc
     6781 cttattctgg tactcaccgg tctgcgcgaa gccgtcatga acgcgatccc cgtcaacctc
     6841 aagcgggcta ttggggtggg catcggcctc ttcatcacga ccatcggcat gaacgagggc
     6901 ggcttcattc gtcccgcgcc catcac
//
LOCUS       DASRMJ010000005         4152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_253164, whole genome shotgun sequence.
ACCESSION   DASRMJ010000005 DASRMJ010000000
VERSION     DASRMJ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253164"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VFD74_00085"
     CDS             complement(<1..940)
                     /locus_tag="VFD74_00085"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZK47990.1"
                     /translation="MYDLEDSRYVVRGGRRLAGVVTVSGAKNSALKLMAASLLADGTS
                     VLHNVPDIEDVRTMSDVLEYLGARVRFDSGDMTIDTSAVEPHAAPYELVQKMRASFQI
                     MGPLVARFGRGRVAMPGGCNLGPRKIDFHLRGLEQLGAVVTSDHGFVEAAAAKLVGAS
                     IVLEYPSVGATENLLMASVLAEGVTVIDNAAREPEIVDLGKFLVSMGADVQGAGTPQI
                     IVRGVAGMQPAEHTVIGDRIEAGTFLIAGAITGGDVEVHGVTPHHLDMFLSKLRAAGV
                     QVKDDHRKIRAWGDPQYYTATDVATLPHPGFPTDLQA"
     gene            complement(1021..1458)
                     /gene="atpC"
                     /locus_tag="VFD74_00090"
     CDS             complement(1021..1458)
                     /gene="atpC"
                     /locus_tag="VFD74_00090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HZK47991.1"
                     /translation="MADDRHFLRLEVVSPDGLVFDGDVAMVIVPADRGEMGILPRHAP
                     IVARLSIGEIRIKTLQDTWLSLAVAEGFVKVQFDKVIVLADAAELATEIDVPRAEEAF
                     ARAQERLALLKSGGAPEDDEIDPFRETMALKRAKNRLTVTKKS"
     gene            complement(1462..2880)
                     /gene="atpD"
                     /locus_tag="VFD74_00095"
     CDS             complement(1462..2880)
                     /gene="atpD"
                     /locus_tag="VFD74_00095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZK47992.1"
                     /translation="MNKGRIVEIMGAVIDVGFPDELPAIYNALEIDVTTDAGRIFLVA
                     EVQQHLGDNKVRAVAMDSTDGLARGMEVTDTGQAITVPVGEATLGRLFNLIGDPIDEG
                     EPIPADVERWPIHRQAPGFEDLNPTVEIFETGIKVIDLLAPYVKGGKVGLFGGAGVGK
                     TVLIQEMIHNLAIEHGGISVFCGVGERTREGNDLYLEMKESGVLAKTALMFGQMNEPP
                     GARLRVGLSGLTMAEYFRDVQGQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPT
                     LASEMGELQERITSTSRGSVTSIQAIYVPADDLTDPAPANSFAHLDATTVLSRSISEK
                     GIYPAVDPLDSTSRALSRGIVTDEHYDTAAAVQEILQRYKDLQDIIAILGMDELSDED
                     KLVVQRARKVEKFLSQPFNVAETFTGRPGKYVKLADTIKGFKEIVEGKHDDHPEQAFY
                     MVGAIEEVVSTARRMRGQSEEE"
     gene            complement(2947..3834)
                     /gene="atpG"
                     /locus_tag="VFD74_00100"
     CDS             complement(2947..3834)
                     /gene="atpG"
                     /locus_tag="VFD74_00100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HZK47993.1"
                     /translation="MASQRDIKRRITSVHNTKKITKAMEMVAAARLRRAQMRIEATRP
                     YAVRMMEFIAGLARYIEVDRARYPLLKQHDEVRRVALVALTADRGLCGAFNSNIVRLA
                     QERRREYESQGIEVDLIVVGKKGVGTFRFQNVAVAGSYVGITDRPAFLDAQALANRVA
                     ELYTTEAVDRVILIFNHFVSAMEQRVTEQVMLPLQEQVVEAYTPDGAAPHMDFLFEPQ
                     ADVILNDLLPSYVEMTVYRALLESTASEHGARMTAMRNASESAGEMIDRLTLAMNRAR
                     QASITQEILEVVAGADALS"
     gene            complement(3850..>4152)
                     /locus_tag="VFD74_00105"
     CDS             complement(3850..>4152)
                     /locus_tag="VFD74_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK47994.1"
                     /translation="RLVETLNQPQYDPWPVEDQVAIIFAATKGYLDDIEVAKVPEFNQ
                     GLRDFLKSGHAAIGDTIRTSKELSDDTTAKLNEAIRAFKADFVGGQAPVPGTPVEA"
BASE COUNT          665 a         1374 c         1342 g          771 t
ORIGIN      
        1 gcgcctggag gtcggtgggg aagccgggat gcggcagtgt cgcgacatcc gtcgccgtgt
       61 agtactgcgg atcgccccag gcacgtatct tgcggtggtc gtccttcact tgaacgcccg
      121 cggctctgag tttgctgagg aacatgtcga ggtggtgggg agtgactccg tgcacctcca
      181 cgtcgccgcc ggttatcgca cccgcgatga ggaacgtgcc cgcctcgatg cggtcaccaa
      241 tgaccgtgtg ctcggccggc tgcatgcccg cgacgccacg cacgatgatc tggggcgtgc
      301 ccgctccttg cacatcggcg cccatgctca cgaggaactt gcccagatcc acgatctcgg
      361 gttcgcgggc cgcgttgtcg atcaccgtga cgccttcggc cagcaccgac gccatcagca
      421 gattctcggt ggctccgacg ctcggatact ccagcacgat ggaggctccc accaacttgg
      481 ccgccgccgc ctcgacgaac ccgtggtcgg aagtcacaac ggcccccaac tgctcgaggc
      541 cgcgcaggtg gaagtcgatc ttgcggggtc ccagattgca gccgccgggc atggcaacgc
      601 gaccccggcc gaaccgagcc acgagcggcc ccattatctg gaacgaggcg cgcatcttct
      661 gcacgagttc atagggagct gcgtggggct cgaccgccga cgtgtcgatg gtcatgtccc
      721 ccgagtcgaa gcgcacgcgg gcgccgagat actcgaggac atcactcatg gtgcgcacgt
      781 cctcgatgtc ggggacgttg tgaagcacac tcgtgccgtc ggccaggaga ctggcggcca
      841 tgagtttgag agccgagttc ttggctcccg agaccgtgac aacaccggcg agacgccgcc
      901 ccccgcgcac tacgtagcgg gagtcctcca ggtcatacat gctgcgagac taaccctttc
      961 acgaagcgag ggcaacccca ggggtcgccc tcgccgattc cgctgctgtg ttggcggccg
     1021 tcagctcttc ttggtcaccg tcaggcggtt cttcgcacgc ttcaaagcca tggtctctcg
     1081 gaacggatcg atctcgtcgt cttcgggggc gccgccgctc ttgagcagag ccagccgctc
     1141 ctgagcgcgg gcgaacgcct cttcggcccg gggcacgtcg atctcagtgg cgagctcggc
     1201 cgcgtcggcc aagacgatga ccttgtcgaa ctgcactttc acgaaaccct cggcgaccgc
     1261 tagcgacaac cacgtgtctt ggagggtctt gatccggatc tccccgatcg acaggcgcgc
     1321 cacgatgggc gcatgccgcg gcaggatgcc catctcccca cgatccgcgg ggacgatgac
     1381 catcgccacg tcgccgtcga agacgagccc gtccggcgag accacttcca ggcggaggaa
     1441 gtgtctgtcg tcggccacgc gctactcctc ctcggactgg ccgcgcatgc ggcgcgcggt
     1501 ggagaccacc tcttcgatgg cacccaccat gtagaacgcc tgctccggat ggtcgtcatg
     1561 tttgccctcg acgatctcct tgaaaccctt gatcgtgtcg gccaacttca cgtacttgcc
     1621 cgggcggccg gtgaaggtct cggccacgtt gaacggctgc gagaggaact tctccacctt
     1681 gcgggcgcgc tgcacgacga gcttgtcctc gtcggagagc tcgtccatgc ccaagatggc
     1741 gatgatgtcc tgaaggtcct tgtagcgctg caggatctcc tgcaccgcgg cggccgtgtc
     1801 gtagtgctcg tcggtcacga tgccgcgcga gagcgcgcgg ctcgtggagt cgagcgggtc
     1861 gacggccgga tagatgccct tctccgagat ggaccggctg agcaccgtgg tggcgtcaag
     1921 gtgtgcgaag gagttggccg gcgctgggtc ggtgaggtcg tccgcaggca cgtagatggc
     1981 ctggatggac gtgaccgagc cgcggctggt ggaggtgatg cgctcctgga gttcgcccat
     2041 ctccgaggcc agagtgggct ggtagcccac ggccgagggc atgcgaccga gaagggccga
     2101 cacctcggaa cccgcctgca cgaagcggaa gatgttgtcg atgaagagga gcacgtcttg
     2161 cccctgcacg tcgcggaagt actcggccat ggtgaggccc gacaacccca cgcgcaggcg
     2221 cgctcccggg ggctcgttca tctgcccgaa catgagggcg gtcttggcga gcacgcccga
     2281 ctccttcatc tccaggtaga ggtcgttgcc ctcacgggtc cgctccccca ccccgcagaa
     2341 cacggagata ccgccgtgct cgatggccag gttgtggatc atctcctgga tgagcaccgt
     2401 cttgcccacg ccggcaccgc cgaagagacc caccttgccg cctttgacgt atggagctag
     2461 caggtcgatc accttgatgc ccgtctcgaa gatctcgacg gtggggttca ggtcctcgaa
     2521 gccgggagcc tgacggtgga taggccagcg ttccacgtcg gcggggatag gctccccttc
     2581 atcgatggga tcgccgatca ggttgaagag gcgcccgagg gtggcctcgc ccaccgggac
     2641 ggtgatggcc tggccggtgt ccgtgacttc catgccccgg gcgaggccgt cggtggagtc
     2701 catggccacg gcgcgcacct tgttgtcgcc caagtgctgc tgcacctcgg ccacaaggaa
     2761 gatgcgcccc gcatcggtgg tcacatcgat ctcgagggcg ttgtagatgg cggggagttc
     2821 gtcgggaaac cccacgtcga tcacggcccc cattatctct acgatcctac ctttgttcat
     2881 cgtgtcagct ttcctccctc ttccaggggc gcgagcctcg cccgaccccg gtcatggtcg
     2941 ttcgggtcag gatagggcgt cggcccctgc cacaacttca aggatctcct gggtgatcga
     3001 cgcctgtcgc gcccggttca tggccagggt gagcctgtcg atcatctcgc ccgcgctctc
     3061 ggacgcgttg cgcatcgcgg tcatgcgggc tccgtgctcg ctcgcggtgc tctcgagcag
     3121 cgcacggtag acggtcatct ccacatagct cggcagcagg tcgttcagga tgacgtcggc
     3181 ctgcggctcg aaaaggaagt ccatgtgcgg cgcggcgccg tcaggcgtgt aggcctccac
     3241 cacctgctcc tgcagaggaa gcatcacctg ctcggtgacc cgctgctcca tcgcgcttac
     3301 gaaatggttg aagatgagaa tcacgcggtc aaccgcctcg gtggtgtaga gctcggctac
     3361 ccggttcgcc agcgcctgag cgtcaaggaa agcggggcga tcggtgatgc ccacgtagga
     3421 gccagccacc gcgacgttct ggaagcggaa ggtgcccacc cccttcttgc ccactactat
     3481 caggtcgacc tcgatgccct ggctctcgta ttcgcgcctg cgttcctgag ccaggcggac
     3541 gatgtttgag ttgaacgccc cgcacagacc gcggtccgcg gtcaacgcca ccagggcgac
     3601 ccggcgcact tcgtcgtgct gcttgagcaa cgggtagcgc gcacgatcca cctcgatgta
     3661 cctagccaga cccgcgatga actccatcat gcgcacggcg tagggtcggg tggcctcgat
     3721 ccgcatctgc gctctgcgca ggcgcgcggc ggccaccatc tccatggcct tggtgatctt
     3781 cttggtgttg tgtacggagg tgatcctccg cttgatgtct ctctgtgaag ccatctgctc
     3841 ttctgggccc taggcctcta ccggcgttcc cggcaccggg gcttgcccgc cgacgaagtc
     3901 cgccttgaac gcgcggatgg cctcgttcag cttcgccgtc gtgtcgtcgg agagctcctt
     3961 gctggtgcgg atggtgtcgc cgatcgcggc gtgaccggac ttgaggaagt cacgcaaccc
     4021 ttggttgaac tcagggacct tcgccacctc gatgtcgtcg aggtagccct tggtcgccgc
     4081 gaagatgatg gccacctggt cctccaccgg ccacggatcg tactggggct ggttgagggt
     4141 ctccaccaga cg
//
LOCUS       DASRMJ010000006         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_144794, whole genome shotgun sequence.
ACCESSION   DASRMJ010000006 DASRMJ010000000
VERSION     DASRMJ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144794"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            499..1620
                     /locus_tag="VFD74_00110"
     CDS             499..1620
                     /locus_tag="VFD74_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZK47995.1"
                     /translation="MAWARKRTTADGNLTFRGYYRDPDGRTRSAGSFPSSRAALRAAA
                     REQGKVSDGTWLDPALGKITFRDFVETVWFPSRHLEPTTKVAYRSYLDKHFLPAFGTR
                     PIARVLPSTVQEWVTKAVEDGLSPQSVVKYHVMLHSVFKRAVRDRVIPFNPCEETELP
                     KVIARRTRTLTPEEYRTLLHEIPDRFKDLIVTDIETGLRWGELIALRPRHIDFLRRTI
                     NVQETIVEVSKKHSPTGERMILKPYPKNDKPRVLGVRQELLDLLAARIARQGLARDDL
                     LFPSTEGGKSALSRNTFRTRVWLPAVERAQIDFPVRVHDLRHAHASWLLAGGADLKSV
                     MDRMGHAQIMTTQKYLHALPDADGKNLSAFDRILNRGTQ"
     gene            1749..2045
                     /locus_tag="VFD74_00115"
     CDS             1749..2045
                     /locus_tag="VFD74_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK47996.1"
                     /translation="MSNTAPLRLVSTTTMPRRTQSREIERPVISITLQADDRRVLTVA
                     EAGHALGISRSKLYQFIAAGEIRTIHIGRLRKVPVEALDEFIATRPPGRSTHAG"
     gene            complement(2057..>2341)
                     /locus_tag="VFD74_00120"
     CDS             complement(2057..>2341)
                     /locus_tag="VFD74_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK47997.1"
                     /translation="VVSEPRSLRPENFAVTAGQRVPDHVLVIDDSWVSGGHAQSVASA
                     LKSSGVADVSIFTVARVLDPQWSPNADFIKERLRGVFDPRICPWTGGDCP"
BASE COUNT          438 a          840 c          705 g          358 t
ORIGIN      
        1 atcctggatc catcgatgaa gtcggtgtgg gaggcgcggc gtgcgtctct ggatggcggg
       61 accagaacgc ggaagtgcca accaccgctt agcgccacct aagtgacggc gacggtgttc
      121 gcgtcgagct cgccaacttc cgcgcgatat ccgccggtgt ccaccggcat ccgcccccgt
      181 ccgccgacga cgtctgccca cattctgccc acatgcacga aagaggccct cccgatctct
      241 cgatcgaaag ggcctctgac ctgcacgttc acttgtagcg ggggcaggat ttgaacctgc
      301 gacctctggg ttatgagcaa aatcccgaga gcggtccggg cgtttcgctt gtgcaccaac
      361 cggggtagga tcacggtatc accgcaggcc agaggcgcta ttcgcagtcc acaagcatcc
      421 atccgcgtcc agcgtcgtgt ggcagcgttt gcggtcacag tgcggtcacg aacagggcaa
      481 cgcttccagg aggcgacaat ggcgtgggct cgcaagcgaa ccacggctga cggcaacctc
      541 accttccgcg gctactaccg ggacccggac ggccggaccc ggtcggcggg ctcgttcccc
      601 tccagccgcg cggcactgcg agccgcggca cgggagcagg gcaaggtcag cgacggtaca
      661 tggctggacc cggccctcgg caagatcacc ttccgcgact tcgtcgagac cgtctggttc
      721 cccagccggc acctcgaacc cacgaccaag gtcgcctacc ggtcctacct cgacaagcac
      781 ttcctgcccg cattcgggac caggcccatc gccagggtcc tcccctcgac cgtccaggaa
      841 tgggtgacca aggcggtcga ggacggtctc tcaccgcaga gcgtcgtgaa gtatcacgtc
      901 atgctccact cggtcttcaa gcgggccgtc cgcgaccggg tcatcccctt caacccttgc
      961 gaggagaccg agctgccgaa agtcatcgcc cgccgcacca ggacgctgac acccgaggag
     1021 taccggacgc tcctgcacga gatccccgat cgcttcaagg acctcatcgt caccgacatc
     1081 gagaccggcc tgcgctgggg ggagttgatc gcactgcgcc cgcgacacat cgacttcctg
     1141 cgccggacca tcaacgtcca ggagacgatc gtggaagtgt ccaagaagca ctcgcctacc
     1201 ggcgagcgga tgatcctcaa gccctacccc aagaacgaca agccgcgggt cctcggcgtc
     1261 cgacaggaac tccttgactt gctcgccgcg cggatcgccc ggcaggggct cgcgcgcgac
     1321 gacctgctct tcccctcaac cgaaggcggg aagagcgcac tgtcgcgcaa cactttccgc
     1381 acccgggtgt ggttgcccgc cgtcgaacga gcccaaatcg acttcccagt ccgcgtccac
     1441 gacctgcgcc atgcccacgc ctcgtggcta ctggccggcg gcgcggacct caagagcgtg
     1501 atggaccgga tgggccacgc tcagatcatg accacccaga agtacctgca cgccctgccg
     1561 gacgccgacg ggaagaacct gtccgccttc gaccgaatcc tgaaccgcgg tacccagtga
     1621 ctgccgcggg tcacgcgggc ctcgggagcg tcatcgcgga ccgatgacgc tccaccctcc
     1681 acccgccgtc atcgcctcgg ctatcgccca tccgcaacat ggtggcccga ctccaggagg
     1741 aggtcaccat gtccaacacc gcacccttgc gactcgtctc gaccacgacc atgcctcgcc
     1801 gtacccagtc acgcgagatc gagcggcccg tcatcagcat cacactgcag gcggatgacc
     1861 ggagggtgtt gaccgtggcc gaggccggtc acgctctggg catcagccgg agcaagctgt
     1921 accagttcat cgccgccggc gagatccgca cgatccacat cggacggttg cgcaaggttc
     1981 cggtcgaggc actcgacgag ttcatcgcca cccgaccgcc gggacgctca actcacgcgg
     2041 ggtgaaacag acgcctctac gggcagtctc cgcctgtcca tggacagata cgcgggtcga
     2101 acacgccccg gagccgctcc ttgatgaagt cggcgttcgg cgaccactgc gggtccagca
     2161 cccgggccac ggtgaagatc gagacgtctg cgacgcccga agacttgagc gcggaggcca
     2221 ccgattgcgc gtggcctccg ctgacccacg agtcgtcgat gaccagaacg tgatcgggca
     2281 ctcgctggcc ggcggtgaca gcgaagttct caggccgcaa cgaccgtggt tcgctcacaa
     2341 c
//
LOCUS       DASRMJ010000007         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_470178, whole genome shotgun sequence.
ACCESSION   DASRMJ010000007 DASRMJ010000000
VERSION     DASRMJ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470178"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFD74_00125"
     CDS             complement(<1..730)
                     /locus_tag="VFD74_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK47998.1"
                     /translation="MTGSSVALRIDASRTGVSPLAESIDCIAREAGVDVVGFGDVREA
                     LPVDFQHLPVGVSLGVAHPAMTLLRERSSAVSEKALQRALSDHRDAQGQAVLETALRR
                     IADHLRSRGYRYFCCPPEVDPMETPFAALMAGRFSHKAAATCAGLGNVGRHGLLNHRD
                     FGPHMVWATVVTNAPIPTGSPVRESECGSCRLCVAACPGGAISGRPWRRDDGMQRLVD
                     VAKCREVLDENERATGRRVCGRCAV"
     gene            complement(946..>3553)
                     /locus_tag="VFD74_00130"
     CDS             complement(946..>3553)
                     /locus_tag="VFD74_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZK47999.1"
                     /translation="LSVSADGLAVLSGHPELRPDAYEQARLSTSRSGRLGAGFMTLLR
                     DLLPLVSDDDFQDAASMAEDFDADVDVAIAFLIHAAAIVSRGGMPLLRAYHEGGVALS
                     RVDWRTARGFLEAVGGAAEGMAHGVAAGRAAGRAAGVSAGGRGPRDRDLGAAELRADD
                     LPPLVDVSARAAARSRHAAKGLFRMLPALASDMTVSGVVEWMDEGLRLTQREDDLVLY
                     MSYGPRRRHEAIEVVCRTASFSAYRGRIALILEAFLGRPAIVRSMFDLLDPSSVPSDI
                     PAFSDGGRLYVRPTMACGSLPPFSLYKLVALHSAAHERFTPYDDPGVQGLLAGGEGRG
                     GAPASASAAATTAAAGTGTTAAATAVSDLDRFLFAVAEDFRVDSALLRTLPGLRSDAE
                     RVVRDTYARYSGAGAAPLSPASLRAHAAAFRFGVGVLRPTELAKSVENILEPLSAPTS
                     LPEDSLRAARSLEALVGDRLMEFKLADPAEWSAAPGGDVPHPPYYDHLFLGMKIASLS
                     GSSGRSEPGAGLIPGTVVDVPGSLHPSETVDGMSISVRGEQGEDDPFELDVDEADAAD
                     AGDADGTVYTYPEWDFQQGGYRPDWCTVRCRAIPGGDPAFVSDTIAHYRGEVHLIRRQ
                     FERLNPDRITRFFRQQEGDELDLDALVDALADRAAGAPMSDKVFVRRDKKQRDVAVLF
                     LLDMSDSTDQLVGAGLRVVDVEKQGLVLLSEAVGRLGDRFAIMGFASRGRRRVDVFQV
                     KRFEEDFDRAVAGRISGVRPSGYTRLGAALRHAADQVGRVDAGVRLLVLLSDGRPYDL
                     GYGDLRYAIEDTKAALAEARRSGTKVFCVTVDPEGPTYLENMFGANRYTVIQDVAHLP
                     TRLPRIYRNLTA"
BASE COUNT          597 a         1298 c         1140 g          518 t
ORIGIN      
        1 ccacggcgca ccgcccgcat acacgtcggc ccgtcgcgcg ctcgttctca tcgagcacct
       61 cgcgacactt ggccacgtcc accaatcttt gcatgccgtc atcccgcctc catggtcgtc
      121 ccgagatcgc tccccccggg cacgccgcca cgcagaggcg acagctaccg cattcactct
      181 cgcgcaccgg ggagccggta gggatagggg cgttggtgac gaccgtcgcc cacaccatat
      241 gcgggccgaa gtcacgatga ttcaagagtc cgtgtctgcc gacgttcccc aacccggcgc
      301 aggtggccgc cgccttgtga gagaagcgcc ccgccatgag agcggcgaag ggcgtctcca
      361 tcgggtccac ctcgggggga caacagaagt agcgataccc gcggctgcga aggtggtcgg
      421 cgatccgccg aagcgctgtc tcgaggacgg cctgcccttg cgcgtcgcgg tggtcgctca
      481 aggccctctg cagagccttc tcggagacgg cggaggagcg ttcgcgcagc agggtcatgg
      541 ccggatgcgc gactcccaga gagaccccca cgggcagatg ctggaaatcc acgggcagcg
      601 cctcccgcac gtctccgaac ccgacgacgt ccacgcccgc ttcccgggcg atgcagtcga
      661 tggactcggc taggggcgac accccggtcc gagaagcgtc tatgcggagc gcaaccgagc
      721 ttccagtcac aatggcctga tcccttcgga tcgcgagttc gcctgcaggt tggatcgcga
      781 gttggactgc gggtcggccc gcgtgttcgc ccgcgtgttc gcccgcctct tctccagcgc
      841 aactcctacc atagcaccgt tccggtgcca tccgggacac ctcttcgtcc ttggcgacga
      901 ccgcggcaga ctcgagccct cggcacttgt gtctcgttcc gcgcatcacg ccgtcaggtt
      961 ccggtagatg cgtgggagtc tggtgggcag gtgtgcgacg tcctggatca cggtgtatcg
     1021 gttcgccccg aacatgttct ccagatacgt ggggccctcg ggatcgacgg tgacgcagaa
     1081 gaccttcgtg cccgaccgcc gtgcctcggc caaggccgcc ttggtgtcct cgatggcgta
     1141 gcgcaggtcg ccgtacccaa ggtcgtaggg gcggccgtcg ctcagcagca cgagaagcct
     1201 cactcccgcg tccactcgac ccacttggtc ggcggcgtgc cgaagggccg ctcccaagcg
     1261 ggtgtagcct gacggtcgca ccccggagat gcgccccgcc accgcccgat cgaagtcctc
     1321 ctcgaacctt ttgacctgga acacgtcaac gcgcctgcga ccgcggctcg cgaaacccat
     1381 gatcgcgaac cggtcgccga gtctaccgac cgcctccgag agcaacacca gcccttgctt
     1441 ctccacgtcc acgacccgca ggccggcgcc caccagctgg tccgtggagt cgctcatgtc
     1501 caggaggaag aggacggcga catcacgctg cttcttgtcc cggcgaacga acaccttgtc
     1561 gctcatcggc gctcccgccg ccctgtcggc cagtgcatcc accagcgcgt cgaggtccag
     1621 ctcgtcacct tcctgctgcc ggaagaaccg cgtgatgcgg tcggggttca gccgctcgaa
     1681 ctgccgccgg atgagatgca cctctccccg gtagtgggcg atcgtgtcag acacgaaggc
     1741 cggatcgccg cccgggatgg cgcggcatct cacagtgcac cagtccggtc tgtaaccacc
     1801 ctgctggaag tcccactccg ggtatgtgta gaccgtcccg tccgcgtctc ccgcatccgc
     1861 cgcatccgcc tcgtccacgt cgagttcgaa gggatcgtcc tcgccctgct ccccccggac
     1921 actgatcgac ataccatcca cggtctcact gggatggagg ctcccgggca cgtccacgac
     1981 cgtaccgggg atgagcccgg cgcccggttc cgacctgccc gaagacccgc tcagcgaggc
     2041 gatcttcatt cccaggaaga ggtgatcgta gtagggagga tggggaacgt cgccccccgg
     2101 ggccgcactc cactcggcgg gatccgcaag cttgaactcc atcaggcgat ctcccaccag
     2161 cgcttcgagg cttcgcgccg cgcgcaggga gtcctcgggc agcgaggtcg gagcagagag
     2221 cggctccagg atgttctcca ccgacttcgc caactcggtg ggacgcagca ccccgacccc
     2281 gaagcggaac gccgccgcgt gggcgcgtag agacgcgggg gagagcggcg cggcaccagc
     2341 gccggagtag cgcgcgtatg tgtcgcgcac caccctctcc gcgtcgcttc ggagccccgg
     2401 caacgtgcgc aggagggcgg agtcgacacg gaagtcctcc gccaccgcga acaagaagcg
     2461 atccaggtcg gacacggcgg tggcggcggc agtggtcccc gtgccggcgg cggcggtggt
     2521 ggccgcagcg gaagctgagg ccggcgcgcc ccctcgcccc tcgccacccg cgagcagacc
     2581 ctgcactccc ggatcgtcgt agggcgtgaa acgctcgtgg gcggcggagt ggagcgctac
     2641 cagcttgtac agtgagaacg gcggcagcga gccgcaggcc atcgtgggcc gcacgtacag
     2701 tcggccgccg tcactgaaag ccgggatgtc cgagggcacg ctcgaggggt caaggaggtc
     2761 gaacatgctc cgcacgatcg cgggccggcc gagaaacgcc tccaagatga gcgctatcct
     2821 gccgcggtag gccgagaacg aagcggttcg gcacaccacc tctatggcct cgtgcctcct
     2881 cctcggcccg taggacatgt acagcacgag gtcgtcctcg cgctgggtca gacggagacc
     2941 ctcgtccatc cactcgacga caccggagac cgtcatgtcc gaggccaacg cgggaagcat
     3001 ccggaacagc cccttcgccg cgtggcggga acgcgccgct gcgcgggcgc tcacatcgac
     3061 gagcggcggc agatcatcgg cgcgcagttc cgcagccccc agatcacgat cccgcggccc
     3121 acggccgccc gccgagacgc cggccgccct gccggccgcc ctgcctgcgg cgacaccgtg
     3181 cgccatcccc tccgctgccc caccgacggc ctccaagaac ccccgcgccg tgcgccaatc
     3241 gacgcgcgaa agcgcgaccc cgccctcgtg ataggcgcgc agcaacggca tcccgccgcg
     3301 cgacacgatc gccgccgcat ggatcaggaa ggcgatagcc acgtcgacgt ccgcgtcgaa
     3361 gtcctcggcc atcgacgccg catcctggaa atcgtcatcc gacaccagcg gcagaaggtc
     3421 gcgcaacagg gtcatgaagc ccgcgcccag gcgtcctgag cgactggtgg ataaacgggc
     3481 ctgctcgtac gcgtcgggcc tcaactccgg gtgaccggat aggaccgcca acccatcggc
     3541 actgacggag agg
//
LOCUS       DASRMJ010000008         3450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_36692, whole genome shotgun sequence.
ACCESSION   DASRMJ010000008 DASRMJ010000000
VERSION     DASRMJ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36692"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFD74_00135"
     CDS             complement(<1..233)
                     /locus_tag="VFD74_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48000.1"
                     /translation="MPLRLIEATVPNEELEKVPGLLDEIRVIHLWTSGWASEPGPEPE
                     LASESASGSRPTSRSADATGLVRVLVDADSDASS"
     gene            complement(284..808)
                     /locus_tag="VFD74_00140"
     CDS             complement(284..808)
                     /locus_tag="VFD74_00140"
                     /inference="COORDINATES: protein motif:HMM:NF021561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HZK48001.1"
                     /translation="MSGDIRFEHLLDSLLAEGESALPDSGVAARLVPISAIAFDERTI
                     LKCRYSCPAWGVRWTCNDNAWGPRELIPLLQKYERVAVLTGMDGNELFPAALALERTA
                     FAAGYYWALAVAVTPCYDCAECAYPADECRHKPDLRPESAMAGIDTMKTLDSLGIRRR
                     RGKRFLRASFVFLE"
     gene            970..1149
                     /locus_tag="VFD74_00145"
     CDS             970..1149
                     /locus_tag="VFD74_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48002.1"
                     /translation="MAKKSSSPRRARSQSVAPGRPGFRGFWDRLSSRQRGQFTMSAGA
                     SVLLILFLIVRALFF"
     gene            1168..3444
                     /locus_tag="VFD74_00150"
     CDS             1168..3444
                     /locus_tag="VFD74_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZK48003.1"
                     /translation="MAEVSGIAELGGGAIVPRIQRLIELAAGLVGAQIAVLVIGTGPG
                     VLSGELPLSLVATHGLDEEAVAGLEDGLRVMVPGLRRRGEAVFQFGSLAAWCALITHA
                     GVYRALELGKGQDYQGFLFLASGEGASLPSAEAPLLALVAQSMETAVEGAVGMGAIAA
                     REEEIRALQEIGKEISALTGLDQVLLLICRKAAELLGVQISYVALADKAQTEIRMRMT
                     YGISESWRYRMWMRFGQGVGGIVAQTRRPLIIEDYGAFDHPTRDDIRRMVLEERIKAV
                     VAVPMVVGDSLTGVLYAADRRSAHFSDHDARLLQGLADQAAIAITNSELYEQEHREVV
                     VHDRLMAISLRNGDYQELAEALHPLAGNPIALYDSHLNLLACHPARDGSDRSGGHDLK
                     EALRSVDSEKAAELLSLFDGHRQGFIMGEDVSLGLERSRAVAPALSGDQLLGYVHIIE
                     EERKLDLHDLRVAERAAVILALRVMRERAEAEVEQRLRGELLDDLLVTDADTVEAAVR
                     RSVHLGHDLQGGQVVVVVEAPSSPASSPSEPWDSEALREGLLDHVKRGLRTRGTQALT
                     GLKGERIVSLVSPGSGAADVDPRALGQFLTDLLERYQPSVPLLAGVGGVCHDPHDFRR
                     SYEEALVCVRVARSRAGARLVVYQDLGLIPLFFDSEHPELLSRFVESRLGSLLEYDRQ
                     HRSALVRTLSTYLDLACNKVAAAGKLNVHLSTLKYRLERIQSVLEVDLADPEARFETH
                     LAVKALGADRLLGRPRRG"
BASE COUNT          531 a         1141 c         1174 g          604 t
ORIGIN      
        1 gagctggcgt cggagtcggc gtcgacgagg acacggacca agccagtggc atccgccgat
       61 cgggaggtcg gcctcgatcc cgacgccgac tccgacgcca gctccggctc cggccccggc
      121 tccgacgccc acccggatgt ccacaggtgg atgacccgga tctcgtcgag cagcccgggg
      181 accttctcga gttcctcgtt cggaaccgtg gcttcaatca gacgaagcgg catctacttc
      241 ccgacctcat tgggttaggc gtagcatagc ctgcgtgccg cccctactcc aagaacacga
      301 agctggcgcg caggaatctc ttcccgcgac gccggcggat gccgagggaa tcgagagtct
      361 tcatcgtatc gatcccggcc atggccgact ccgggcgcag gtcgggcttg tggcggcact
      421 cgtcagccgg gtaggcgcat tccgcgcagt cgtagcaagg ggtcacggcc acggccagcg
      481 cccagtagta gccggcggcg aaggcggtgc gctccagtgc gagagcggcc gggaagagtt
      541 cgttgccgtc catacctgtg agcacggcca ccctctcgta cttctgcaga agggggatca
      601 gttcgcgcgg accccaggcg ttgtcgttgc aggtccagcg gacgccccac gccgggcagg
      661 agtagcggca cttgaggatg gtgcgctcgt cgaaggcgat cgcgctgatg ggcacgagcc
      721 gcgccgccac gccgctgtcg gggagagcgc tttcgccctc agcgagcaga gaatcgagaa
      781 ggtgttcgaa gcggatgtca ccactcatgt gcggcctccg ggagtcgctg ccctgtgtcg
      841 actcgattct accccgtggg gcacgtcggc caatgagcgt ttcttgcctt ccaccgtcat
      901 gcgcgctagg gttcaggtct ccgatccgcg attgcccaac gacatccgca gtctggtagg
      961 aggtagagga tggccaagaa gtcgtcgagt ccccgccgcg cccgctcgca gtcggtcgcc
     1021 ccgggtcgcc ctgggttccg aggattctgg gaccgcctct cgtcgcggca gcgcggccag
     1081 ttcaccatgt cggcgggcgc gtccgtgctc ctcatcctgt tcctgatcgt tcgcgccctc
     1141 ttcttctgac ccggtctgct gtagatcgtg gctgaggtct cgggaatcgc ggaactcggt
     1201 ggcggggcca tcgtcccccg catccagcgg ctcatcgagt tggcggccgg tctcgtcggg
     1261 gcgcagatag cggttctcgt gatcgggaca ggcccggggg tcctctcggg cgagctgcct
     1321 ctctcgctgg tggcgacaca cggcctcgac gaagaggcgg tcgccgggct cgaggacggt
     1381 cttcgagtga tggtgccggg actccgtcgc cggggcgagg cagtcttcca gttcggctct
     1441 ctcgccgcct ggtgtgccct catcactcat gccggcgtgt accgggctct cgaactgggc
     1501 aagggccagg actatcaggg gtttctgttc ctggcgtcgg gcgagggcgc ttcgctcccc
     1561 tccgccgagg ctccactctt ggcgctcgtc gcgcaatcca tggagacggc cgtcgagggt
     1621 gccgtcggta tgggggccat cgccgcgcgt gaggaggaga tccgggccct gcaagagatc
     1681 ggcaaggaga tctcggcctt gacgggactc gaccaggtgc tgctcctcat ctgtcgcaag
     1741 gcggccgaac tcctcggggt gcagatctcg tacgtcgccc tggcggacaa ggcccagacc
     1801 gagattcgca tgcgcatgac ctacggcatc agtgagtcct ggcgctatcg gatgtggatg
     1861 cgcttcgggc agggggtggg cgggatcgtc gcccagaccc gtcgcccgct catcatcgag
     1921 gactacggcg ccttcgacca cccgacccgg gacgacatac gccggatggt ccttgaagag
     1981 cgcatcaagg cagtggtggc cgtcccgatg gtggtgggcg actcgctcac cggcgtgctc
     2041 tacgccgcag accgccgctc tgcccacttc agcgatcacg acgctcggct gctccagggt
     2101 ctggccgatc aagcggccat cgccatcacc aactccgagc tgtatgaaca ggagcaccgg
     2161 gaagtcgttg tgcacgaccg cttgatggcc atctcgttgc ggaacggaga ctaccaggag
     2221 ctggccgagg ctctgcaccc cctggcgggc aaccccatcg cactctacga cagtcatctg
     2281 aaccttctag cctgccatcc cgcccgggac ggcagcgacc ggagtggggg gcacgacctg
     2341 aaggaggccc tccgctctgt cgacagcgag aaggccgcgg agctgctctc cttgttcgac
     2401 ggccacaggc aggggttcat catgggggag gacgtgagct tggggctgga gcgaagcagg
     2461 gcggtggcgc cggcgctctc cggcgatcaa ttgctgggct acgtccacat catcgaggag
     2521 gagcggaagc tggatctcca cgacctacga gtagccgagc gcgcggccgt catcctcgct
     2581 ctgcgcgtca tgcgcgagcg cgcggaggcc gaggtggagc agaggctgcg aggcgaactc
     2641 ctcgacgatc tgctggtcac cgacgccgat accgtggagg cggcggtccg aagatcggtg
     2701 cacctggggc acgacctaca gggaggccag gtcgtcgtcg tggtggaagc gccctcgtct
     2761 cccgcatcgt cgccttcaga accgtgggat tcggaggcgc tgcgcgaggg actgctcgac
     2821 cacgtgaagc gtggactgcg gacgaggggt acccaggccc tcaccggcct gaagggcgag
     2881 cggatcgtct cgctggtgag ccccggcagc ggcgccgcgg atgtcgatcc tcgtgcactc
     2941 ggccagttcc tcaccgatct cttggagcgc taccagccgt cggtcccgct gctcgcgggc
     3001 gttggcggtg tctgccacga tccgcacgac ttccgccgaa gctacgagga ggctctcgtg
     3061 tgtgtccgcg tggcgcgatc gcgcgccggg gcgcgcctcg tcgtctacca ggacctcgga
     3121 ctgatccccc tgttcttcga ttccgaacac cctgagctgc tgtctcggtt cgtcgagtcc
     3181 cgcctgggaa gcctactcga atacgatcgg cagcatcgta gcgcgctcgt gcggacgctc
     3241 tcgacgtatc tcgatctcgc ctgcaacaag gtggcagccg ccggcaagct gaatgttcat
     3301 ctcagcacgc tcaagtatcg cctggagcgg atccagtcgg tgctggaggt cgacctcgcc
     3361 gaccctgagg cccgcttcga gacgcatctt gcggtcaagg cgctcggggc cgaccgcctg
     3421 ctcggtcgac cgcgccgggg ctgatagtcc
//
LOCUS       DASRMJ010000009         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_289580, whole genome shotgun sequence.
ACCESSION   DASRMJ010000009 DASRMJ010000000
VERSION     DASRMJ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289580"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFD74_00155"
     CDS             complement(<1..404)
                     /locus_tag="VFD74_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZK48004.1"
                     /translation="MARPLRRTRIVATLGPACDRDSVIEGMLVAGMDVARINFSHGTH
                     EDHARRIEAVRRVAAGMGRPVAVLQDLQGPKIRVGAVEGGEVCLVAGALVELVPDDGS
                     LGTATRLGISLSSLGRQLRPGAVVLIDDGRVR"
     gene            complement(407..1699)
                     /gene="eno"
                     /locus_tag="VFD74_00160"
     CDS             complement(407..1699)
                     /gene="eno"
                     /locus_tag="VFD74_00160"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZK48005.1"
                     /translation="MTVILDIYGRQILDSRGNPTVEVEVELASGARGRASVPSGASTG
                     QFEAVELRDGDRAYGGKSVLRAVANVNEAIADALVGMDALGQREIDHMLIDLDGTPNK
                     GNLGANALLGTSLAVARAAATAVGLPLYSYLGGVNAHQLPVPMMNILNGGKHADNNVD
                     LQEFMCMPVGADTFADALRMGVEVFHALKEVLSSRGLNTSVGDEGGFAPNLSSNEEAI
                     KVILEAVEKAGYTPGEEIFIALDPAATEFFNEVTGLYELTSEGRTLTPVEMAGYYADL
                     CGRYPIISIEDGMAEEDWEGWAAITAALGERVQLVGDDLFVTNVERLARGIEAGVANS
                     VLIKVNQIGTLSETLDTMSMAHRAGYTTVVSHRSGETEDSTIADLAVATNAGQIKAGA
                     PSRGERTAKYNQLLRIEDELGDVAVYPGMRAFTSLRRA"
     gene            complement(1696..>2544)
                     /locus_tag="VFD74_00165"
     CDS             complement(1696..>2544)
                     /locus_tag="VFD74_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HZK48006.1"
                     /translation="SSAGSSVDRSSASPDASPDAGASDADLIRGELGDLLFQVNFLAC
                     VAEESGAYDLGHVAAGIREKLVRRHPHIFGEAEANTPDDVRRTWDDIKRNTEGRIGIF
                     HEVPQALSAALFAQKLQQRAATVGFDWREAADVFDKIREELDELEEAVRAHRGTADGR
                     AGVPADAAASGRGPAAALGGFDASAGHERELRDRVEAEVGDLLFASVNLARKLKVDPE
                     LALRGAAHRFRARVEAAAGLAGVQGSRFEDMDLEEQETYYQRAKKGIKDGRGAPADGT
                     QGSVGP"
BASE COUNT          384 a          919 c          811 g          430 t
ORIGIN      
        1 aaacgcaccc gaccgtcgtc gatgagcacc accgccccgg gtcgcaactg ccggccgaga
       61 gacgacagcg agatccccag gcgggtcgcg gtgcccaggg agccgtcatc gggcaccagt
      121 tccacgagtg cgcccgcgac cagacacacc tcgccgccct cgaccgcgcc taccctgatc
      181 ttcgggccct gcagatcctg caggacggcg accggtcggc ccatccccgc ggccacgcgg
      241 cgcaccgcct cgatgcgccg cgcgtggtct tcgtgggtgc cgtgggagaa gttgatgcgg
      301 gccacgtcca tgccggcgac caacattccc tcgatgaccg agtcacggtc gcaggcaggt
      361 cccagcgtcg cgactatccg ggtgcgccgg agcggtctcg ccacgatcaa gctcgacgaa
      421 gactcgtgaa cgccctcatc cccggataca cggccacgtc gcccaactcg tcctcgatgc
      481 ggagcagttg attgtacttg gccgtgcgtt cgccccgtga cggcgctccg gccttgatct
      541 ggccggcatt ggtcgcgacg gcaaggtcgg cgatggtgga gtcttccgtc tcgccgctgc
      601 ggtggctcac gaccgtggtg tagccggccc gatgggccat gctcatcgtg tcaagggtct
      661 cggaaagggt gccgatctgg ttgaccttga tcaacacact gttggccacg cccgcctcaa
      721 tgccccgggc caggcgctcc acgttggtga cgaagaggtc gtcacccacg agctgcaccc
      781 gctcaccaag ggcggcggtg atggcggccc agccctccca gtcctcctcg gccatgccgt
      841 cctcgatgct gatgatcgga tagcggccac acaggtccgc gtagtagccg gccatctcga
      901 ccggcgtgag cgtgcggcct tcgctcgtga gttcgtagag ccccgtcacc tcgttgaaga
      961 actcggtggc ggccggatcg agagcgatga atatctcttc gcccggggtg tatcccgcct
     1021 tctcaaccgc ctcgaggatc accttgatgg cctcctcgtt gcttgacaag ttgggtgcga
     1081 agcccccttc gtctcccacg gacgtgttca gaccgcggct cgacaacacc tccttcaggg
     1141 cgtggaacac ctcgacaccc atacgcagcg cgtcggcgaa cgtgtcggcg cccacgggca
     1201 tgcacatgaa ctcctgcagg tcgacgttgt tgtcggcgtg cttgcccccg ttcagtatgt
     1261 tcatcatggg cacggggagt tggtgcgcgt tgactccacc caagtagctg tacagcggca
     1321 ggccgacggc agtggcggcc gcgcgggcca ccgccaggga ggtgcccagg agcgcgttgg
     1381 cgccgagatt gcccttgttg ggagtgccgt caaggtcgat aagcatgtgg tcgatctcgc
     1441 gctgacccaa ggcatccatg cccaccagcg cgtcggcgat ggcctcgttc acgttcgcga
     1501 cggcgcgcag gacgctcttc cccccgtagg cgcggtcgcc gtcgcgcagc tccactgctt
     1561 cgaactgacc ggtggaggca cccgacggca ccgaagcacg cccccgagca cccgaagcga
     1621 gctccacctc tacctctacc gtcgggttgc cccgcgaatc gaggatctgc cgaccgtaga
     1681 tatccaggat caccgtcatg gtcccacgct cccttgcgtt ccgtccgcgg gcgcgccgcg
     1741 cccgtccttg atgcccttct tcgccctctg atagtaggtc tcttgctcct ccaggtccat
     1801 atcttcgaaa cgcgacccct ggaccccggc cagtccggcg gccgcctcca cgcgggcccg
     1861 gaaccggtgt gcggcccccc gcagagccaa ctccgggtcc accttgagct tgcgggcgag
     1921 attgacggag gcgaacagta ggtcgcccac ctcggcctcc acccggtcgc gcagctcccg
     1981 ctcgtgcccg gcggatgcgt cgaacccgcc gagggcggcg gccggtcccc ggccggatgc
     2041 ggccgcgtcg gccggcacgc ccgcacggcc gtccgccgtg ccgcgatgcg cccgcacggc
     2101 ctcctccaac tcgtcgagct cctcccggat cttgtcgaac acgtcggcgg cttcccgcca
     2161 atcgaacccg accgtcgcgg ccctctgctg cagtttctgc gcgaagaggg cggccgaaag
     2221 agcctgcggc acctcgtgga atatgcctat ccgcccctcg gtattgcgct tgatgtcgtc
     2281 ccaggtgcgg cgcacgtcgt cgggggtgtt cgcctcggcc tctccgaaga tgtggggatg
     2341 acggcgaacc agcttctcac ggatgccggc cgcgacgtgg cccaagtcgt acgcgcccga
     2401 ctcctccgcc acgcaggcca gaaagttcac ttggaagagg aggtcaccga gttcgccccg
     2461 gatgaggtct gcgtcggacg cgccggcgtc ggggctggcg tcggggctgg cgctggacct
     2521 atcgacgctg gagccggcac taga
//
LOCUS       DASRMJ010000010         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_253439, whole genome shotgun sequence.
ACCESSION   DASRMJ010000010 DASRMJ010000000
VERSION     DASRMJ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253439"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFD74_00170"
     CDS             <1..185
                     /locus_tag="VFD74_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZK48007.1"
                     /translation="PKSIRDRFGFDPESEVDFVVRDGAVLLVHREAPRKEAVHRLYGR
                     KNLGATTDELMKLLRP"
     gene            194..589
                     /locus_tag="VFD74_00175"
     CDS             194..589
                     /locus_tag="VFD74_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019502253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZK48008.1"
                     /translation="MVDTNVILDIIGADDEFGGRSRDALAEAAETGALVVNTVVFAEV
                     GSVVDTLEELNDLIPESVFRRDTIPWEAAFLAGRAFHAYRRSGGERDRMLPDFLIGAH
                     AAVGGFGLITRDKGQARHFKVEILDPADR"
     gene            655..900
                     /locus_tag="VFD74_00180"
     CDS             655..900
                     /locus_tag="VFD74_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HZK48009.1"
                     /translation="MKTTIDIAEPLLDQAKTVARAEGTTLKRLVEEGLRLALERHADG
                     LSFQLRDASYQGDGVQSGVDLTQWDQLRDAIYRGRGS"
     gene            897..1331
                     /locus_tag="VFD74_00185"
     CDS             897..1331
                     /locus_tag="VFD74_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HZK48010.1"
                     /translation="MIAVDTNLLVYAHRRDVEWFPRASECLRSLAQGSEPWAVPWPCA
                     HEFLAIVTHPRIFAPPTPVELALDQLAAWMESPSLVILGEARGYWDVFAELVAKARVA
                     GPLVHDARIAALCLHHGVDELWSADRDFGRFPALRVRNPLAA"
     gene            1557..1748
                     /locus_tag="VFD74_00190"
     CDS             1557..1748
                     /locus_tag="VFD74_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HZK48011.1"
                     /translation="MQVREVRAILEADGWELVRTRGSHRQYRHPTKAGRVTLAGNAKD
                     DLAAGTLSSILKQAGLKRR"
     gene            1751..1963
                     /locus_tag="VFD74_00195"
     CDS             1751..1963
                     /locus_tag="VFD74_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZK48012.1"
                     /translation="MHKYLVIVERAERNYSAYSPDLPGCVATGDTREEVERRMAEAMR
                     MHVDGLREDHLPIPEPCSFAEYISVA"
BASE COUNT          337 a          611 c          667 g          396 t
ORIGIN      
        1 ttccgaagag tattcgtgac cggttcgggt tcgaccccga gtcggaggtg gactttgtgg
       61 ttcgagacgg cgcagtgctc ttggtccacc gcgaggcgcc gcgcaaggag gccgtgcatc
      121 gcttgtatgg gcgcaagaat cttggcgcaa cgaccgatga actgatgaag ctgctgcggc
      181 cgtgagagag tggttggtcg acacgaacgt gatcctcgac atcatcgggg ctgatgatga
      241 gtttggtgga cgttcccgtg atgcgcttgc tgaggctgct gagacggggg cgctcgtggt
      301 caacacagtg gtgttcgctg aggtcgggtc agtcgtcgac acgctcgaag agctcaatga
      361 cctcattccc gagtccgtat tccgtcggga cacgatcccc tgggaggcgg cgttcctagc
      421 aggtcgggcc tttcacgcgt accgccgttc tgggggtgag cgcgaccgca tgctgcccga
      481 cttcctgata ggcgcgcacg cagcggtcgg cggattcggg ctgatcacga gggacaaggg
      541 ccaggcccgc cacttcaagg tggagatcct tgaccctgcc gatcggtagc tgggcgggga
      601 cgatgcccgg gcttggcggt cttcgatgcg cgaccgtgtg atatggttag caatatgaag
      661 acaaccatag atattgccga accacttctt gaccaggcca agacggtggc cagagcggag
      721 gggaccaccc tcaagcgtct ggtcgaggag ggcctgcgcc tggccctcga gcgtcacgcc
      781 gacggcctct ccttccagct tcgcgacgcc agctaccagg gagacggcgt gcagtccggc
      841 gtcgacttga cgcagtggga tcaacttcgc gacgccatct accggggacg cggatcatga
      901 tcgccgtcga caccaatttg ctcgtctatg cacaccgccg cgacgtcgag tggtttcccc
      961 gagcctccga gtgcctccgc agtctagctc agggttcaga accgtgggcc gtgccctggc
     1021 cctgcgcgca tgagttcctc gctatcgtca cccatccccg catctttgct ccacccacgc
     1081 ccgtcgagct ggctttggat cagctggcgg cttggatgga gtcgcccagc ctcgtgatcc
     1141 tcggcgaggc gcgcggatat tgggatgtgt tcgccgaact cgttgcgaag gcacgggtcg
     1201 cgggcccgct ggtgcacgat gcgcgtattg ccgccctgtg tcttcatcac ggcgtggacg
     1261 agttgtggag tgcggaccgg gacttcggtc gcttcccggc cctccgggtg cggaatccgc
     1321 tggcggcata ggcaggtctc accccacctt cttcggaatg actgttggcc tgactgtgga
     1381 gcggctgcct ggtcggtcgc ggatccccgt ggcaccgatt tccccctcgc gggcatcagc
     1441 cagcctcctc cctcaggtgg atgggctagt cgcgtccccc ggcgcaccat tgggcagatc
     1501 gttgacggcg gtcgaagccg aaccgccggg ctctgccgac attattgggg tacgatgtgc
     1561 aggtacgcga agtcagggcg attctcgaag ccgacgggtg ggaactcgtg agaacgaggg
     1621 gaagccaccg tcagtatcgt catcccacaa aggcgggtag ggtcacactg gctgggaacg
     1681 cgaaggacga cctcgccgcc gggactttga gcagcatcct gaagcaggct ggtctgaaga
     1741 ggaggtagac ttgcacaagt acctcgtgat cgtcgagcgg gccgaacgca actattcggc
     1801 ttactccccc gatctccccg gctgtgtcgc caccggtgat actcgtgaag aggtggagcg
     1861 gcgcatggct gaggccatgc ggatgcatgt ggacgggctc cgcgaggatc atcttcccat
     1921 tcccgagcct tgctctttcg cggagtacat ctccgtcgcg taggccacgt gcgggaggga
     1981 atccttctca ccccaccttc ttcggaatag c
//
LOCUS       DASRMJ010000011         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_398357, whole genome shotgun sequence.
ACCESSION   DASRMJ010000011 DASRMJ010000000
VERSION     DASRMJ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398357"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFD74_00200"
     CDS             <1..400
                     /locus_tag="VFD74_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK48013.1"
                     /translation="TIPAATFSDVPSGAGYPFVYVEAAAAAGIVKGFPTATPSEVPRF
                     GPYENVLRIQVAQMLARAGSEKLDPPPPGIVHPFYDAPSYAEAELAPIWQMGIIKGRS
                     SVVYDPWTPATRGQVAEMLFRLRSVFQARP"
     gene            635..1408
                     /locus_tag="VFD74_00205"
     CDS             635..1408
                     /locus_tag="VFD74_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZK48014.1"
                     /translation="MAIEPSSKPMNLEGKVALVTGAGSGIGRAISRRFGALGAKVLVT
                     DVNEAGGAETVQMVVAAGGVAGFFRCDVTSFADAKAAVAAARETFGRLDVLVNNAGWD
                     IIEPFLKNDPQQWTRVIDINLKGPIHMSRAALEAFVEQEIGGKIVNLASDAGRVGSMG
                     EAVYSACKGGVIALTKTLAREGARYRVQVNAVAPGITDTPLVAGLDPKVIEAIVKSTP
                     MRRMADPDEPAEMVAFLASDSNTFMTGQVISVSGGLTMV"
     gene            1447..2085
                     /locus_tag="VFD74_00210"
     CDS             1447..2085
                     /locus_tag="VFD74_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48015.1"
                     /translation="MALYAVGGNSPRIHPAAFVHPSASIIGRVEVGPGCYIGAGASLR
                     GDWVTIKIGAGSNVQDYCTIHGAIGATVELAEDSHLGHGCVVHGARLERDVLVGMNAV
                     VLDGAVLGEGSIVGSGCVVPSGLEVPPRMLVVGVPGRVTSEVRPEMARRKQAGTRWYQ
                     QLAARCLLDFREIEAAMCGDGWEDNTWGGPEVWTAWFDELTKDELTKEDPAG"
     gene            2150..2935
                     /locus_tag="VFD74_00215"
     CDS             2150..2935
                     /locus_tag="VFD74_00215"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZK48016.1"
                     /translation="MNGLEGAAGTSIPRRLEGRVAIVTGAGEGIGRAVAVRFAQEGAR
                     VVVASRRREPLEETRAEIEAVGGEALVVPTDVSDWDAVQAMSAATIDAYGQVDILVTI
                     AGFTKPAMLHRMSVDDWHAVMGVHLHGTFYCIRSVVENMIEREHGRIITVSSAAGLVG
                     TLGQVNYSAAKGGVVALTKSCARELGKYRITANCVAPGAETKMTDTIVNDPKFKDKYL
                     ARIPLGYWAKPEEVAPTFAWLASDDAAYTTGYVIGADGGLSIH"
BASE COUNT          493 a          974 c         1071 g          478 t
ORIGIN      
        1 cacgataccc gcggccacgt tctccgatgt gccgtcgggc gcgggctacc cgttcgtcta
       61 cgtcgaagcc gccgcggccg cgggtatcgt gaagggcttc cccaccgcga ctccctcaga
      121 agtgccccga ttcggtccat acgagaacgt cttgcgcata caggtggcgc agatgctcgc
      181 gcgcgccggg agcgagaagc tcgacccgcc gccgcccggg atcgttcatc cgttctacga
      241 cgccccttcg tatgcggagg ccgaactggc gcccatctgg cagatgggga tcatcaaggg
      301 gcgctcttcc gttgtctacg acccgtggac ccctgcgacc aggggacagg tggccgagat
      361 gctcttccgc ctgcggagtg tctttcaggc tcggccgtag ccggcgcttc gcctcgcggt
      421 cgcgcgcggt gccgcacgag ccttggtcgc cgctgtcctg actttcggtg ccggcggcga
      481 gctgatcgcc gccgcgcacg cccgggaact tgcctgcgcg gttgacatgc tcgggggcgt
      541 gcactagcat gagtcgagag ttgactgacc gaccgaccaa attccggctc cccctgtggg
      601 gcgcccgaga atacccgcga aagaagggga tacgatggcc atagaaccga gcagcaaacc
      661 catgaacctc gagggcaagg tcgccttggt cacaggagcc gggagcggta tcggtcgggc
      721 catctccagg cgcttcggcg cgctcggcgc caaggtgctc gtgaccgacg tcaacgaagc
      781 aggtggcgcg gagaccgtgc agatggtcgt cgcggccggc ggcgtcgccg gcttcttccg
      841 atgcgacgtg acctccttcg ctgatgcgaa agcggccgtc gccgccgccc gtgagacctt
      901 cggccgactc gatgtgctgg tgaacaacgc cggttgggac atcatcgagc ccttcctcaa
      961 gaacgatccg cagcagtgga cccgggtcat cgacatcaac ctcaaaggcc ccatccacat
     1021 gagtcgcgcg gctctggagg ccttcgtcga gcaggagatc ggcggcaaga tcgtgaacct
     1081 ggcctccgat gcgggccgcg tcggatccat gggcgaggcg gtctactccg cctgcaaggg
     1141 tggcgtcatc gctctcacca agacgctggc ccgcgaaggc gcccgctacc gcgtgcaggt
     1201 gaacgccgtg gcgccgggga tcaccgacac gcctctcgtg gccggtctgg atcccaaggt
     1261 catcgaagcc atcgtcaaga gcaccccgat gcgtcgcatg gccgatcccg acgagcccgc
     1321 ggagatggtg gcgttcctcg ccagcgactc gaacacgttc atgaccggcc aggtgatctc
     1381 ggtgagcggc gggctcacga tggtgtgacc cgcctgccgg acgcacccta ggaggtacgg
     1441 gcccccatgg cgctgtacgc ggtcggaggc aactcgccgc gcatccaccc ggctgcgttc
     1501 gtccacccga gcgcttcgat catcggccgg gtggaggtgg gccccggctg ttacatcggg
     1561 gcgggagcga gtctgcgcgg ggattgggtc accatcaaga tcggcgccgg ctcgaacgtc
     1621 caggactact gcaccatcca cggcgccatc ggggcgacgg tggagttggc ggaggactcg
     1681 cacctggggc acggctgcgt ggtccacggc gcccgcctcg agcgggacgt gctggtgggc
     1741 atgaacgccg tggtcctcga cggggcggtc ctcggcgagg gttctatcgt gggttcggga
     1801 tgcgtggtgc cgagcgggct ggaggtgccg ccccgcatgt tggtcgtggg ggtgccgggc
     1861 agggtcacgt cggaagtgcg gccggagatg gcacgcagga agcaggccgg cacccgctgg
     1921 tatcagcagt tggccgcgcg ttgcctcctt gactttcgag agatcgaggc cgcgatgtgc
     1981 ggcgacggtt gggaggacaa cacgtggggg gggcctgaag tgtggactgc ctggttcgac
     2041 gagctgacga aggatgagct gacgaaagaa gatccggccg gctgagtgcg gcacgctcga
     2101 tcgtgatgat gatgcggctc cggccgcgat gcgaggagga atccgaccta tgaacggact
     2161 tgaaggagca gcgggcacgt cgatcccccg ccgactcgaa ggccgcgtgg ccatcgtcac
     2221 cggggcggga gagggcatcg gacgtgcggt ggcggtgcgc ttcgcccagg aaggtgcgcg
     2281 ggtcgtcgtg gccagtcgcc ggcgcgagcc gttggaggag accagagcgg agatcgaagc
     2341 ggtgggcggc gaggcgctcg tggtgcccac cgacgtctcc gattgggacg cggtgcaggc
     2401 gatgtcggcg gccacgatcg acgcgtacgg ccaggtggac atcctcgtga ccatcgccgg
     2461 gttcaccaag cccgctatgc tccaccggat gtcggtggac gactggcacg cagtgatggg
     2521 cgtgcacctg cacggcacct tctactgcat ccgctcggtg gtggagaaca tgatcgagcg
     2581 cgagcacggc cgcatcatca cggtgagttc ggccgccggc ctcgtgggca ctctcggcca
     2641 agtgaactac tctgcggcga agggcggcgt ggtcgccctt accaagtcgt gcgcgcgcga
     2701 actcggcaag taccgcatca ccgccaactg cgtggctccc ggcgccgaga ccaagatgac
     2761 ggacactatc gtcaacgatc ccaagttcaa ggacaagtac ctggcgcgca tcccgctcgg
     2821 atactgggct aagccggagg aagtagcccc taccttcgcg tggctggcct ccgacgacgc
     2881 cgcgtacacc accggctacg tcatcggagc agacgggggt ctctctatac attgacacga
     2941 cctgagcgcg tgcactcggt agctacggcc ggaggcccgc cgccccgccg gcgcgggcgg
     3001 ggggtcagcc cgtgac
//
LOCUS       DASRMJ010000012         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_361951, whole genome shotgun sequence.
ACCESSION   DASRMJ010000012 DASRMJ010000000
VERSION     DASRMJ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361951"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..643)
                     /locus_tag="VFD74_00220"
     CDS             complement(194..643)
                     /locus_tag="VFD74_00220"
                     /inference="COORDINATES: protein motif:HMM:NF023374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HZK48017.1"
                     /translation="MLAVAIVAVCLLGAAQSGCSATTTPTGVGSDSQVKSAAEADDRF
                     ARAFAERAVDRELEGVGTVSKLLTDDTDGAEHQRFIVRLDSGQTVLVTHNVDVAPRVR
                     GLGVGDTVAFKGEYVWNAQGGLMHWTHHDPSGSHIAGWIKHDGRTYQ"
     gene            complement(661..876)
                     /locus_tag="VFD74_00225"
     CDS             complement(661..876)
                     /locus_tag="VFD74_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HZK48018.1"
                     /translation="MTSQRWTDMNDARKVLVLTAAAVQFALLGAALWDLRRRDASLVR
                     GPKRLWRGVVFVNWAGPIAYFTVGRKS"
     gene            complement(938..1504)
                     /locus_tag="VFD74_00230"
     CDS             complement(938..1504)
                     /locus_tag="VFD74_00230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HZK48019.1"
                     /translation="MADPLRLLGFAGSLRRGSYNRAALHVAAELAPVGMEVEIFDLTP
                     IPFYNADVEAEEPPPNVVDFKERIARSDALLIATPEYNYSIPGVLKNAIDWASRPADL
                     SPLKGKPVALLGAGGRMGTARSQMHMRQVAVFTGMQVMVDPQVYIADARGQFDEHGHL
                     TNELYREQIRALMAALWDWTLLIQGRSS"
     gene            1687..2307
                     /locus_tag="VFD74_00235"
     CDS             1687..2307
                     /locus_tag="VFD74_00235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK48020.1"
                     /translation="MLEEDRPKMGNSSLRHIRGHRREEYFDRHLRCFALAEQFDVPMV
                     YHAGLTLHGEFVWDCLRVHPAVRVDIPHFGLSRTVMASFFEEFPLLFSDIAGLETHIT
                     AGPDFYGMFFNEFCDRIMLGSDSVVGIGLERVPRYARVVRNLLLPPTAERAILSANAA
                     RFLGGRLWGCADLHAHLMAHVALVSVQIPVFRIRRGPRRRDPRPKT"
     gene            complement(2301..2609)
                     /locus_tag="VFD74_00240"
                     /pseudo
     CDS             complement(2301..2609)
                     /locus_tag="VFD74_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015207.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
BASE COUNT          464 a          898 c          807 g          498 t
ORIGIN      
        1 cctcgggccc gtgctcgcgg tcctgggcga caccgacccg gccggggtca tcagcacccg
       61 agcggaagtc gcactgcggg agttgctcgc actgaagcat aggcagccac ccgagtgacc
      121 ggcaggccgg gccgatccca cccaccgacc gggtcccccg agccgcagcc gcgcggtcta
      181 cgcgggttcg cccctactgg tatgtccggc cgtcatgttt gatccaaccc gcgatgtgac
      241 tccccgaggg gtcgtgatgc gtccagtgca tgaggccgcc ctgcgcgttc cacacgtact
      301 cgcccttgaa ggcgacagta tcaccgaccc ccagccctct cacccgtgga gcgacgtcca
      361 cgttatgggt cacgagcacg gtctgacctg aatccagccg cacgatgaac cgctgatgtt
      421 cagcaccgtc ggtatcgtcg gtcagcaact tggataccgt tccgactccc tcgagttccc
      481 ggtcgacggc ccgttctgcg aaagcacgcg cgaaacggtc gtcggcctcg gcagcggatt
      541 tgacttggct gtcagagccg acgccggtcg gcgtggtggt cgccgaacag ccagattgcg
      601 cagcgcccag cagacagact gccacgatgg cgaccgcgag caacagcctg ctcgaaggct
      661 tcagctcttc ctgcccacgg tgaagtaggc gatcggtccc gcccagttga caaagacgac
      721 gccgcgccag agccgcttgg gtccgcgcac caacgaggcg tcgcgtcggc ggagatccca
      781 aagcgcggcg cccagcagcg cgaactggac cgccgccgcg gtgagcacga gtaccttcct
      841 tgcgtcgttc atgtcggtcc accgttgcga agtcattctt cctcctcact ttcctctcga
      901 actcacagcc gccggggccg cgacacactg agccaggcta cgaagaacgc ccctggataa
      961 gcagggtcca gtcccacagt gccgccatca gagcgcggat ctgctcgcgg taaagttcgt
     1021 tcgtgaggtg cccgtgttcg tcgaactgac cgcgcgcatc ggcgatatat acctgcggat
     1081 cgaccatgac ctgcatcccg gtgaacacgg ctacctggcg catgtgcatc tgggagcggg
     1141 ccgtacccat gcgtccccct gctcccagca aggccaccgg cttgcctttc aacggtgaga
     1201 ggtcggccgg acgcgacgcc caatcgatgg cattcttgag cactccgggt attgagtagt
     1261 tgtactccgg agtcgcgatg agcagggcgt ccgaccgagc gatccgctct ttgaaatcga
     1321 ccacgttcgg agggggttcc tctgcctcca cgtcggcgtt atagaatggg ataggcgtaa
     1381 gatcgaagat ctcgacttcc atacccacgg gtgcaagctc agccgccacg tgcagggccg
     1441 ccctgttgta tgacccgcgc cggaggctcc cggcaaagcc caataagcgc agcggatccg
     1501 ccatctcccc acctccgccc tcactgggcg ctcgcaatgc agcaccgagg tactctgccc
     1561 agaagacacg gcgcgtagtc gcccgcaccc gtcgccgggg atgtgcctcg aaggcccgcc
     1621 gccgccaggc gttggagacc cgggggttgg gtagtcggta cccaggctgg atccaagaac
     1681 atcctcgtgc tggaagaaga ccggcccaag atggggaact cctcactccg ccatattcgc
     1741 ggccacaggc gggaggagta cttcgaccgt cacctgcgtt gcttcgctct cgcggaacaa
     1801 ttcgatgtgc ccatggtcta ccatgccggc ctcacgctgc acggcgagtt cgtgtgggac
     1861 tgtctcaggg tgcaccccgc agtgcgcgtg gacatcccgc actttggact cagccgcacg
     1921 gtgatggcca gcttcttcga agagttcccg ttgctcttct cggatatcgc cggcctcgag
     1981 acgcacatca cggccggccc cgacttctac ggcatgttct tcaatgagtt ctgcgaccgg
     2041 atcatgttgg ggagcgacag cgtggtgggc atcgggctcg aacgtgtccc gcgctatgca
     2101 cgggtggtgc gaaacctcct gcttccgccg actgccgaac gggcgatcct gtccgccaac
     2161 gccgcgcgct tcctgggggg ccgcttatgg gggtgcgccg atctgcacgc ccatctgatg
     2221 gcccacgtgg ccttggtttc cgtccagatt cccgtcttca ggattcgtcg agggcctaga
     2281 agacgagatc ctagaccaaa gacataaccg acccaccact gtgcccaccg gagcctgctc
     2341 ggcctgaacc cgtactcgca gagcttgagc tggaagtcct gcatgtgggt catctcgttg
     2401 aacatggcgg cggccagctg ggtgttgagc tcgcaaggct tggtcgtgat ctgagccttg
     2461 tagatgttga cggccatcac ttcgttggca tgcagggtgc caagcgcctt ccttatctcc
     2521 ttccggcgct ttggcggata gtcgcggcct aaggccctga cgttatcgat ccgcacctcg
     2581 gggcggatca ccgaaccctc atcgctcatg gcgacctcct cagatatttg tccacgggaa
     2641 ccgccgagtg ttggtttcgt tctatac
//
LOCUS       DASRMJ010000013         8862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_361952, whole genome shotgun sequence.
ACCESSION   DASRMJ010000013 DASRMJ010000000
VERSION     DASRMJ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8862
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361952"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..687)
                     /locus_tag="VFD74_00245"
     CDS             complement(79..687)
                     /locus_tag="VFD74_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HZK48021.1"
                     /translation="MEEVGRWFDRGHVRRLAATALASRLGGGGAVSTLAAVLGAVVGA
                     VVLSVALAGPAVAAAGELTPAQQQLAADIDGKLIAPCCWTQTVALHESQKAEEIKMQV
                     RLLVAQGKPESEIIDVFVGQYGEQILAAPRAHGFNWLAYLLPFGAIIVGLGAISVLAG
                     RWRGAQPKVVPVPASSSKPAVRDEREEDLRRRLDKDLARFDS"
     gene            complement(766..984)
                     /locus_tag="VFD74_00250"
     CDS             complement(766..984)
                     /locus_tag="VFD74_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK48022.1"
                     /translation="MASYDLKCAGCGREFEVFVQGFLKDAHRVCPDCGSSEVEQRFTG
                     FMMGSMGGSRGPAPAPAGGGCAHAGSFG"
     gene            complement(985..1227)
                     /locus_tag="VFD74_00255"
     CDS             complement(985..1227)
                     /locus_tag="VFD74_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48023.1"
                     /translation="MSLEETIGYINRYLRQDYGDHLAVTYVDVDSPELQEYPALAEAV
                     AEGRSVPLVLAGDTVKSPAVLSFAWFVNEYKMLGVL"
     gene            complement(1344..1670)
                     /gene="trxA"
                     /locus_tag="VFD74_00260"
     CDS             complement(1344..1670)
                     /gene="trxA"
                     /locus_tag="VFD74_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZK48024.1"
                     /translation="MGSGIVEDLNAESFETAVISADGSVVVDFWAPWCGPCKMVGPVL
                     EKLAADHSGTVKVVKVNVDENQELAARYGIMSIPTVMLFEGGAVKQQLVGARSQKDYE
                     REFGIA"
     gene            1998..2594
                     /locus_tag="VFD74_00265"
     CDS             1998..2594
                     /locus_tag="VFD74_00265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZK48025.1"
                     /translation="MDDKIARFLRTAARGPDNRLVRTVAASTLLLLAAALAAVYLFSE
                     GQRAALGPRLWATSAVEAGLAIVLLASALTSLGRAHANWAAGALLTVGSVFAVFATSG
                     AHGILNLAVPMMVFVLAATLPVRTAVFASVVLLSSHLAATVLAATVSGGIATMDAADP
                     DPQSLIERADAALYEAKRAGKNRMHAAGNAVPPITGQA"
     gene            2783..3349
                     /locus_tag="VFD74_00270"
     CDS             2783..3349
                     /locus_tag="VFD74_00270"
                     /inference="COORDINATES: protein motif:HMM:NF018844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HZK48026.1"
                     /translation="MSTPWVVYTLVAVDLAAAIAGYIYWYGSDILASPLYLWPFVPDS
                     PLSGTLVAIALLALHRGRHWEFLGLMAATGAIKYGLWTDWYWFTNVLSGGHYTFEAVH
                     LSASHFAMVIQGLLLLPFLRPRLRHVLFVAAWYGLNDLTDYAFGSHPRIPNPQDLGLI
                     AGFAVVSTIALCSAWLVWALARRRSAAA"
     gene            complement(3372..3581)
                     /locus_tag="VFD74_00275"
     CDS             complement(3372..3581)
                     /locus_tag="VFD74_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HZK48027.1"
                     /translation="MITKTYNVPDVSCGHCKMAIERAIGAMSGVNSVQVDVEKKSVDI
                     DFDEAVVQEAAILARLEEEGYPAAA"
     gene            3724..4050
                     /locus_tag="VFD74_00280"
     CDS             3724..4050
                     /locus_tag="VFD74_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZK48028.1"
                     /translation="MASYVHDKAKLLARLRRIEGQVRGVHKMIDEEKYCVDVLTQVSS
                     VMAALQGVGLIVLEDHIQGCVRGAIESGQGGDQAVSELIDVLQRFVKAGPVPRHPDPT
                     APTPEG"
     gene            complement(4155..4811)
                     /locus_tag="VFD74_00285"
     CDS             complement(4155..4811)
                     /locus_tag="VFD74_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZK48029.1"
                     /translation="MPTQAVEEYLESIYKLNLRGGRVIAARLAEDLSVSAPTATEMLR
                     RLRDAGYVRMGAEKEITLTDAGVAAAEVLVRRHRLSERLLTDILGMPWSRAHEEACKF
                     EHVISPEVEARLARVLDNPSTCPHGNPIPGTGGGGEELVRLSQAPVGSRGQLRCVEAE
                     DEELLSYVERLGLVPGAIVEVLGFSPLEGPVEVRVSDERVSVGLAAAERLLMRLISTA
                     "
     gene            5054..5389
                     /locus_tag="VFD74_00290"
     CDS             5054..5389
                     /locus_tag="VFD74_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HZK48030.1"
                     /translation="MTRILWYVRSDSLGQDAKIGGRLVQSFFNSLGERLLEPADFAFI
                     NRGIFLTLDDSPVLDALRDLESKGCRLFSCGTCLDYYESTDRLAVGTVGSMALLQEWM
                     LGADRVMTL"
     gene            5391..5615
                     /locus_tag="VFD74_00295"
     CDS             5391..5615
                     /locus_tag="VFD74_00295"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HZK48031.1"
                     /translation="MESVVVLTFPSTHQAMAAEDVLRGAGIRIQVIPAPPQRVRGCGL
                     AVRLAGIDVARACSLLESKSVTYQRPGEAP"
     gene            5775..8747
                     /locus_tag="VFD74_00300"
     CDS             5775..8747
                     /locus_tag="VFD74_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HZK48032.1"
                     /translation="MPRNGMEAILMAARWFAGRQGGQGGAGAGPGSSARTAGRRPKRG
                     GRRRIVMVLAVGLPTLFIVLGLLSTFYTELLWYRETGFEGVFLTRIWARLAVGAAGGA
                     VFVVVFFLNVYLARRISPRIRLMGKSGPNDVLELVPTEEGTVTKVLLGITLALGFFFA
                     LGTGNLWQDILLLVNRVPFSYADPVFGRDASFFVFVVPVASSIASFLGFTIFLTFVGT
                     VGVYVFGRAARVEGERRLLLAPHVKAHLSSLAAAGLLVKAADYILSSWKLVWSPRGVV
                     FGASYTDVNAQLPVFRILAVVSVIAAVIFLVNIHYRGWRLPAAAVGLLAIVWLLAGQV
                     YPAVLQQYRVSPNEIVAEGPYIRENIQATRFAFGVADVTPAPFPLGGELTAADIASNA
                     PTIDNVRLWDPRPLLDAYGQLQELRPYYTFHDVDVDRYTIAGDYRQVTLSGRELDQSK
                     LDSRARTWVNDHLTYTHGYGAVVSRVNGATAEGLPDFLVQDIPPRSVHEDLVITRPEI
                     YFGEVSDDYVLVRTAAKEFDYGKGNENVFSTYEGGGGVEINSLVRKIAFSFRFGTLKL
                     LLNNDLRSDSRVMFRRTLASRVQAIAPFLSYDRDPYLVIRDDGSLVWIWDAYTSTDRF
                     PYSQPREGGVNYLRNSIKVVVDTFTGGVTFYQIDPTDAMANTIGAVYPGLFTPGDAMP
                     DDVRRHLRYPEDLFAVQTEVLGVYHMQDEQVFYNKEDVWQIPTEVYSGEETPVLPYYV
                     IMSLPGETQEEFLLLQPFTPMNKKNMISWIAGRMDGEHYGELMVYEFPKDSLVFGPAQ
                     IEARISNDPTISEQLTLWDQAGSQVIRGNLLVIPMEKSLIYVEPLYLQASGENPIPEL
                     TRVIVSYESKVVMEETLTGALEKMFGTADGGTTGGTTGGTTGGGSGGSGGGTTTTTLP
                     TTTTTTLPGATLPTDPAALAALAQQHFEAAVAAQRRGDWAEYGRQIDELGRVLTALEG
                     VANPTTTATTTPTTTTQG"
BASE COUNT         1518 a         3068 c         2770 g         1506 t
ORIGIN      
        1 aggtgcccga ttcccgaccc ggccgagtgg ctctatcgtc tggacgagcg gcgccgcgag
       61 gcacatgcgg cgcccagcct atgagtcgaa tctagcgaga tccttgtcga ggcggcggcg
      121 tagatcttcc tcgcgctcgt cgcgaaccgc cggcttcgac gaggacgccg ggacgggcac
      181 cactttcggt tgagcgccgc gccaccgccc ggccagcacc gatatggcac ccagacccac
      241 gatgatcgcg ccgaacggga ggaggtaggc cagccagttg aagccatgcg cgcgcggcgc
      301 ggcgaggatc tgctccccat actgacccac gaagacgtcg atgatctcac tctccggctt
      361 gccctgagcc acgagaaggc gcacctgcat cttgatctcc tcggccttct ggctctcgtg
      421 cagggcgaca gtctgagtcc aacagcacgg ggcgatgagc ttgccgtcga tatcggccgc
      481 cagctgctgc tgcgcggggg tcagctctcc ggcagccgca acggccggtc cggcgagggc
      541 gacggacagc acgacggccc ccaccactgc tccgagcacc gccgccagag tcgagaccgc
      601 gccgccgcct ccgaggcgag acgccaaggc cgtggcagcc aacctacgca cgtgacccct
      661 gtcgaaccat ctgcccacct cttccacctc caactgagtt cccacgaccg cggcggtagg
      721 acgaagaaag aaaaggggcc caacgggccc ccacgttcag tgtgctcagc cgaagctgcc
      781 tgcatgcgca cagccgccgc ccgcaggagc gggagcgggc cctcttgagc cgcccatcga
      841 gcccatcatg aacccggtga accgctgctc gacctcgctg ctgccgcaat cgggacacac
      901 ccgatgcgcg tccttcagaa agccctgcac gaagacctcg aactcgcggc cgcaccctgc
      961 gcacttgaga tcgtacgatg ccatctacag gacccccaac atcttgtact cgttgacaaa
     1021 ccaggcgaac gacagcacgg ccggtgactt tacggtgtct cctgccagca cgagagggac
     1081 actgcggcct tcggccacgg cctcggccag cgcaggatac tcctgcagct cgggactatc
     1141 cacgtccaca taggtcacgg cgaggtgatc gccgtagtct tgccgcaggt agcggttgat
     1201 gtacccgatc gtctcttcca gagacaacgg gggagctcag ccgccgctcc tgcacgcatc
     1261 caacgcatgg gaaccgacca ccaccatatc gccggtcttg acactactca tggtacctcc
     1321 cgatccagca gtcgacacag cgactaggct atgccgaact cgcgttcgta gtctttctga
     1381 ctccgggccc cgaccaactg ctgcttgacg gcgcccccct cgaagagcat gacggtcggg
     1441 atgctcatga tcccgtagcg agcggcgagt tcctgattct catcgacgtt caccttgacc
     1501 accttcaccg tgccgctgtg gtcggcagcc aacttctcca gcacgggacc caccatcttg
     1561 cacggaccgc accacggtgc ccagaaatcc acgaccactg agccgtccgc gctgatgacg
     1621 gcggtctcga acgattcggc gttcagatcc tcgacgatac cactacccat agcccttgta
     1681 cctctccttc taactggggg acaaccctcc ccaagcgatc aatcgacctg ctggggtgga
     1741 ctcgatcctg cttgagcgag cgacggccct cgaccgtctc catataccca tgggggtatg
     1801 ataccatggc ccggagtgac cggcaagcgc gctccgccgg cgcacctccg agaacgacgc
     1861 gcgaggcggc gacggtgtcc agcctacccc cggtcgactc ggtgctaaca tgcggacgag
     1921 tacctttgac actccaggga atgatacccg gtgtgtccgg gaggcgccaa cccgagagcc
     1981 gacacacggg gatccaggtg gacgacaaga tcgctcgctt cctacgcacg gccgcccgcg
     2041 gtcccgacaa ccgcctggta cggacggtcg ccgcgagtac gctcctgctt cttgccgcgg
     2101 ccctggcggc cgtctatctc ttctcggaag gtcagcgcgc cgctttgggt ccccgactgt
     2161 gggcgacgtc cgccgtcgag gcgggcctcg cgatcgtgct cctggcgagc gcgctcacct
     2221 cgctcggacg cgcgcacgcg aactgggcgg ccggggccct cttgacggtc ggctccgtgt
     2281 tcgccgtgtt cgcaacctcc ggagcgcacg gcatcctgaa cctcgcagtg cccatgatgg
     2341 tgttcgtgct cgcggcgacc cttcccgtac ggacggccgt gttcgcctct gtagtcctgc
     2401 tctcgtcaca tctcgcggcc acggtcctcg cggccacggt cagcggcggg atcgcgacga
     2461 tggacgcggc cgaccccgat cctcagagcc tcatcgagcg ggccgacgcc gctctctacg
     2521 aagcgaagcg cgccgggaag aaccggatgc atgccgccgg caacgccgtc ccacccatca
     2581 cggggcaagc ctgataggga gaccgtccgg cgaggcgctc cgcggacatc ggaacgcctt
     2641 cctcgtgcgc tatactcccg cccgtgaccg agcgcgacga cacccatcga cccgtctcgc
     2701 cccctacggc gcaggatctc cgcaaggcac acatgttcgc ggcgcacgct tcggcgtgtg
     2761 gggttccttc tcccggtggg tggtgtctac cccatgggtc gtctacacat tggtggcggt
     2821 tgacctggcc gccgcgatag ccggctacat ctactggtat ggcagcgaca tcctggcgtc
     2881 gcccctctac ctctggccgt tcgtcccgga tagcccgcta tcgggcactc tggtggcgat
     2941 cgccctgctg gctctccatc gcggcaggca ctgggagttc ttaggcctga tggctgcgac
     3001 cggtgccatc aagtacgggc tctggaccga ctggtactgg ttcacgaacg tgctgtctgg
     3061 ggggcactac accttcgaag cagtccatct gagcgcgagc cacttcgcca tggttatcca
     3121 gggcctgctg ctcctcccct tcttgaggcc tcgacttcgg cacgtcctgt tcgtggcggc
     3181 ctggtacggc ctcaacgacc tcaccgacta cgcgttcggc agtcacccca ggatccccaa
     3241 cccgcaggac ttgggactca tcgccgggtt cgccgtggtc tccaccatcg cgctttgctc
     3301 ggcatggctc gtgtgggccc tcgcgcgaag gagatcagca gccgcgtagc cgagccgcaa
     3361 gaagagggcc tctatgccgc tgccgggtag ccctcttctt cgagtctcgc aaggatggcg
     3421 gcctcttgga cgacagcctc gtcgaagtcg atgtccaccg acttcttctc aacatccacc
     3481 tgcacactgt tcacgccgct catggccccg atcgcgcgct cgatggccat cttgcagtgt
     3541 ccgcagctga cgtcgggcac gttgtaggtc ttggtgatca tcccgcctcc tcgttcgctt
     3601 cttccacatg tgtggttcat ggtatagtac caataccccc tatacctata caagggcggg
     3661 gcggagtctc tgccacggac ataccacttc ggacattcca attcggacgc cacgggaggc
     3721 gcgatggctt catatgtaca cgacaaggcg aagttgctgg cgcgccttcg gcgcatcgag
     3781 gggcaggtgc gcggagtgca caagatgatc gacgaggaga aatactgcgt cgacgtgctc
     3841 acgcaggtct cgtccgtgat ggcggcgttg cagggagtgg ggctgatcgt actcgaggac
     3901 cacatccagg gatgcgtgcg gggcgccatc gaatcggggc aagggggcga ccaggctgtg
     3961 agcgagttga tcgacgttct gcagcgcttc gtcaaagccg gcccggtgcc acgccacccc
     4021 gatccgaccg cgcccacacc cgaggggtag cgcgcgtgac gttgccccgc agcgcgacgc
     4081 gcggggcgcc acacagggtg atggtggagt gatgccacgc ggggaagcat cgccgcgggg
     4141 gctgcgggcg gcggctacgc ggtagagatc agccgcatca ggagtcgttc ggccgccgca
     4201 aggcccacgg agacgcgttc atcactcacg cgcacttcga ccgggccttc cagcggcgag
     4261 aagccgagca cctcgacgat cgcccccggc accaacccca gccgttccac gtagctgagc
     4321 agctcttcgt cttccgcctc gacgcaccgc aactgcccgc gcgaacccac gggtgcctgg
     4381 ctcagcctca cgagctcctc ccccccgcct cccgtcccgg ggatggggtt gccgtgcggg
     4441 caggtggagg gattgtcgag cacgcgcgcg aggcgggcct ccacttcggg cgagatcacg
     4501 tgttcgaact tgcaggcctc ctcgtgcgcc cgcgaccacg gcatcccgag gatgtcggtg
     4561 agcaaacgct ccgacagccg gtgccgtcgc accagcacct cggcggccgc gaccccggcg
     4621 tcggtgaggg tgatctcctt ctcggcgccc atccgcacat agccggcgtc gcgcaggcgc
     4681 cgcagcatct ccgtggccgt gggagccgac acgctgagat cctccgcgag gcgcgccgcg
     4741 atgacccgac ccccgcgcag gttcaacttg tagatggatt cgaggtactc ctcgacggcc
     4801 tgggtgggca tcgccggcga tctcctcccg atgtctcgcg ctcgcagccg ccacaacact
     4861 ggcctgcatg gacggcacgc ggatccgccc ggagtatatc agcggcgtga gatacactcg
     4921 ggatgcaggc atagactcga gatgagagcc tcgccgtgcg cgcagcggtt gctcgcgccg
     4981 gacgaggcgc cgagggtcgg ggcgggcggc gacgagcgac aacggggcga cgacgccgaa
     5041 tgaagggggc aagatgactc ggatcctttg gtacgtgagg tctgacagcc tgggccagga
     5101 tgccaagatc ggcggccggc tcgtccagtc gttcttcaac agcctcgggg agcgcctctt
     5161 ggagcccgcc gacttcgcct tcatcaaccg cggcatcttc ctcaccttgg acgactcgcc
     5221 cgtactggac gctctgcgcg acctggagtc gaagggctgc cgtctcttct cgtgcggcac
     5281 ctgcctcgac tactacgagt cgaccgatcg cctggccgtg gggaccgtgg ggtcgatggc
     5341 tcttctccaa gagtggatgt tgggggccga tcgagtgatg accctctgac atggagtcgg
     5401 tagtggtcct caccttcccc tccactcacc aagccatggc ggcggaagat gtccttcgag
     5461 gcgcggggat ccgcatccag gtgatcccgg caccgcctca acgcgtgcgc gggtgcggac
     5521 tcgcggtgag actcgcgggc atcgatgtcg cgcgggcgtg ctcgcttctc gagtcgaagt
     5581 ccgtgaccta ccaacgccct ggcgaggccc cgtgaccaac cgtggtgatc ggtcctggcg
     5641 cgtcgcgcgt cgcagaaggt agactagagg cgtccggccg cggcgagacg cgtcgccggc
     5701 cccgtcgcag ctctaaggag gaagacatct tttccactaa cggaaccagc ggagggccca
     5761 ccggaggacc ccggatgcca cggaacggta tggaggccat actcatggct gcgcgttggt
     5821 tcgccggccg tcagggggga cagggcggcg cgggcgccgg ccccggctca tcggcgcgca
     5881 ccgcgggccg acggccgaag cggggcggtc ggagacgcat cgtgatggtc ctggcggtgg
     5941 gactccccac cctgttcatc gtcctcggtc tgttgtccac cttctatacc gagctactct
     6001 ggtaccgcga aaccggcttc gagggagtct tcctcacgag gatatgggcc cgactggcgg
     6061 tgggagcggc cggtggagcc gtcttcgtgg tcgtcttctt cttgaacgtc tacttggcac
     6121 gccgcatctc tccccgcatc cggctcatgg gcaagagcgg gcccaacgac gtgctggaac
     6181 tcgtcccgac cgaggagggc accgtcacca aggtgctgct cggcatcact ctggccctcg
     6241 ggttcttctt cgcgctcggg acgggaaacc tgtggcagga catcctgcta ttggtgaacc
     6301 gggtcccgtt cagctacgcc gacccggtgt tcgggcgcga cgcttccttc ttcgtcttcg
     6361 tggtgcccgt ggcgtccagc atcgcgtcgt tccttggctt caccatcttc ctcaccttcg
     6421 tgggcacggt cggtgtctac gtgttcggcc gcgccgccag ggtggagggc gagcgaaggt
     6481 tgcttctcgc accccacgtg aaggcccacc tgtcgtcgct ggccgcggcg gggctcttgg
     6541 tcaaggccgc cgactacatc ctgtcttcct ggaaactggt gtggtccccc cgcggagtcg
     6601 tgttcggcgc cagctacacg gacgtcaacg ctcagctgcc ggtgttccgc atcctcgccg
     6661 tggtctcggt catcgcagca gtcatcttcc tggtgaacat ccactaccgc ggctggcgac
     6721 tgcccgcggc ggcggtgggg ctcctcgcga tagtgtggct tctggcgggt caggtgtatc
     6781 ccgcagtgct ccagcagtat cgcgtctcgc cgaacgagat cgtggccgaa ggcccgtata
     6841 tacgagagaa catccaggcc acgcgattcg ccttcggagt ggcggacgtc acgccggcgc
     6901 cgttccccct gggtggcgag ttgacggcgg ccgacatagc gtccaatgcg cccaccatcg
     6961 acaacgtccg cttgtgggac cctcgacctc tcctcgacgc gtacgggcaa ctccaagaac
     7021 tccgccccta ctacaccttc cacgatgtgg acgtggaccg ctacacgatc gcaggagact
     7081 accgccaggt gacgctgtcg ggtagggaac tcgaccagag caagctcgac tcccgagcgc
     7141 ggacctgggt caacgaccac ctcacctaca ctcacggcta cggcgccgtg gtgagccgcg
     7201 tcaacggagc gaccgcggaa ggcctgcccg acttcctcgt gcaggacatc ccgccgcgaa
     7261 gtgtgcacga ggacttggtc atcacgaggc ccgagatcta cttcggcgag gtgagcgacg
     7321 actacgtgct cgtgcgcacc gcggccaagg agttcgacta cggcaagggc aacgagaacg
     7381 tcttcagcac ctacgagggc ggcggcggag tcgagatcaa ctcgttggtg cgcaagatcg
     7441 cgttcagctt ccgcttcggc actttgaaac tcctcctcaa caacgatctc cgctccgact
     7501 cgcgtgtgat gttccgccgc acgctggcct cgcgtgtgca ggcgatcgcc cccttcctca
     7561 gctacgaccg cgacccctat ctggtcatca gagacgacgg cagcctcgtc tggatctggg
     7621 acgcctacac gagcaccgac cgcttcccct actctcaacc ccgcgagggc ggcgtgaact
     7681 acctgcggaa ctcgataaag gtggtcgtcg atacgttcac cggcggggtg accttctacc
     7741 agatcgaccc cacggacgcg atggccaaca ccatcggggc cgtctatcca ggattgttca
     7801 cgcccggtga cgcgatgccc gacgacgtgc gacgccacct tcgctacccg gaggatctgt
     7861 tcgcggtcca gacggaggtc ctaggcgtct accacatgca ggacgagcag gtcttctaca
     7921 acaaggagga tgtctggcag atccccaccg aggtctactc gggcgaggag acgccggtcc
     7981 tgccctacta cgtgatcatg tcgctccccg gcgagaccca ggaagagttc ctgctcctgc
     8041 aaccgttcac cccgatgaac aagaagaaca tgatctcgtg gatagcggga cgcatggacg
     8101 gcgagcacta cggcgagttg atggtgtacg agttcccgaa ggactccttg gtcttcgggc
     8161 cggcacagat cgaagcccgc atctcgaacg accccaccat ctccgagcag ctgaccctct
     8221 gggaccaggc cggcagccag gtcatccgcg gcaacctgct cgtgatcccg atggagaaat
     8281 cactcatcta tgtggagcct ctctacctgc aggcctccgg cgagaacccc atccccgagc
     8341 tgacccgggt gatcgtctcg tatgagagca aggtggtcat ggaagagacg ctgactgggg
     8401 ctttggagaa gatgttcggc accgccgacg gcgggacgac gggtggaacg accggaggga
     8461 ctacgggcgg cggaagcgga ggttcgggag gcggaacgac caccacgacg ctcccgacca
     8521 ccacgacgac gaccttaccc ggagcgacgc tccccaccga cccggccgcc cttgctgcgc
     8581 tggcgcagca acacttcgaa gcggcggttg cggcccagcg ccgcggagac tgggcggagt
     8641 acggcaggca gatcgatgag ttaggcaggg tgctgaccgc cctcgaggga gtggccaacc
     8701 ccaccaccac tgccaccacc accccaacca cgaccactca gggttgacgc gacgctgagt
     8761 ggccccagac accgatcacg caggagatgc gagtgaaccc gcgcgacaaa gaccagcccc
     8821 ccagcttccg ccgcctccag aacgaggtcg accggatgtt cc
//
LOCUS       DASRMJ010000014         7645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_435078, whole genome shotgun sequence.
ACCESSION   DASRMJ010000014 DASRMJ010000000
VERSION     DASRMJ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435078"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1306)
                     /locus_tag="VFD74_00305"
     CDS             complement(251..1306)
                     /locus_tag="VFD74_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZK48033.1"
                     /translation="MKDIERLLRLFADAHGVSGHEGAVASLLVAELEPLVDEVSTDAM
                     GNVVGTKTGPGPSIMIAAHMDEIGFMVKYIDESGFLRFVPLGGWFDQMVLGQRVLVHG
                     TKGALTGVIGAKPPHIMDDEERKKIVKIKEMFIDIGAIDADDAAALGVEIGSPVTLDR
                     TMATLANGFVTGKSFDNRAGVVMMIAAMQRLKGKKCKATVHAVGTVQEEVGLKGARTS
                     AFSLDPDAAIIADVTVPGDHPGVTKEQRHVMTGKGPVLTVVDSDGRGVIVPKKILNWL
                     RESADSAKIPYQLDVGSGGTTDATAIHLTKKGVPSGVVSIATRYIHAPIEVLSLKDID
                     QGADLLVQALLSAHKHL"
     gene            1494..2294
                     /locus_tag="VFD74_00310"
     CDS             1494..2294
                     /locus_tag="VFD74_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HZK48034.1"
                     /translation="MGGLLEGRRGLIMGVANERSIAWGIAQAARREGAELAFTFAGEI
                     LEKRVRPLAAQLGSGFVLPCDVQVDEDIESVFSAIGAEWGSLDFFVHSVAFANREDLK
                     GSFVDTPRDGFRLALDVSVYSLVASVRAALPYMKPGGSILTMTYLGGERTIPHYNVMG
                     VAKAALESAVRYLAADLGPRGIRVNAISAGPVRTLAAAGIGDFRKILDWYEQNAPLRK
                     ATTPDEVGDAALMLLGDLGRATTGEVIHVDGGYHAVGVPPAVEEHDEG"
     gene            2343..4205
                     /gene="uvrC"
                     /locus_tag="VFD74_00315"
     CDS             2343..4205
                     /gene="uvrC"
                     /locus_tag="VFD74_00315"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZK48035.1"
                     /translation="MDVSELERQIKEAPDLPGVYLFRDGDSAVVYVGKALSLRRRLAS
                     YAPAVREDAHVPVKVGEMARRAESVEWIVTSSDVEALLLEHNLIKRHRPDFNICLRDD
                     KSYPYIVITMEEEFPRVMFTRLPHRRGDLYFGPYGSAAKVRETLDALGRVFPYRKCRG
                     MTPGRRNGSPCLQFHIKRCNAPCVGGVSREEYRAVVARVADLLAGRHLQAVSAMEAEM
                     RVAANARDFEKAALLRDRLEALRHILERQQVQGAVQGAVDIIGLARDEGGANVQVFLT
                     RDGLLSDRRSFTLVGTEGASEAELLERFMGEYYSTAVSVPPEIVVPRTSSDLGNLALF
                     LEGLRGTRVNVRHAERGDKRRLQELADRNAVLALEHERLRSARSRERRLGGLSALQAS
                     LGLDGPPIRIEGYDISNLGSEHIVASMVVFEGGAAKKSDYRKFVIRGLSGQDDFGAIH
                     EAVLRRFTRKTGVDTPEAYDPSFEAVPDLVLIDGGKGQLSAALQALEEAGLDDVVPVV
                     SLAKREEELFVPGRGAPLDVAPDDPGLMLLRSVRDEAHRFAVGFHRARRQATATDSFL
                     DGLPGVGEKRKRAILAHFGSPERFLQATSEEVEAVPGLPGRVARFIYTYVHKNG"
     gene            4219..4791
                     /locus_tag="VFD74_00320"
     CDS             4219..4791
                     /locus_tag="VFD74_00320"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZK48036.1"
                     /translation="MSEATFRTPWGLGRVVVEGEYLVEVVLPRLDAPPVVQGEEPPDG
                     AMTADGGATGAAAASGSDAAVRGALPDPPDPAARWARELDDYFSCGRQGWSDEEVRLE
                     ERRFTPFRTAVYRALLEVPVGSTVTYGELARRVGRPGAARAVGTAMAQNPIAVVVPCH
                     RVVRNDGSLGHYGFGDAWKPLLLQLEGARA"
     gene            4788..5669
                     /gene="rapZ"
                     /locus_tag="VFD74_00325"
     CDS             4788..5669
                     /gene="rapZ"
                     /locus_tag="VFD74_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019331052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZK48037.1"
                     /translation="MTALKDLHFTVITGLSGAGKSQAVATFEDMGFFCIDNLPPQMLA
                     GVFELFSLEGSRVDRLAVVFDVRAAQYFDELMRSLDDLTEGGAQCHILFLEASDEALV
                     ARYQSTRRPHPVAHDGPLLAGIQEERRLLMPLRERADVVIDTTELNVHELRHRLQETL
                     FAGELTNEILVSLISFGFKFGLPAEADMVLDVRFLPNPHWVEELRPLTGLDSAVRDFV
                     VARPESQGFIERAAALLLYLVPLYLAEQKTQLVVAIGCTGGRHRSVALAEALAACLAK
                     EAHVQTAVRHRDVERSG"
     gene            5650..6702
                     /gene="yvcK"
                     /locus_tag="VFD74_00330"
     CDS             5650..6702
                     /gene="yvcK"
                     /locus_tag="VFD74_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HZK48038.1"
                     /translation="MSNVRADPAAVDSARAAYANDPATEAHHPRVVAVGGGTGLSTLL
                     RGLKHLTPNITAVVTVADDGGSSGRLRADLGVPPPGDIRDCLLALADHESLLASLFSH
                     RFASGELAGHSVGNLLLAALTEVLGDFDKAVKESSKVLAITGMVLPGTTSDVRLHALL
                     SDGRHLVGESRIGRSPVSVSRVWLEPEDAQALPLALARIGEADLVVLGPGSLFTSVMP
                     NLLLGGMREALASTEARVVYVANVMTQPGETASFTGRDHLAALIDHLGQGVLDAVLVN
                     STPVPDGALERYGCAGAEPVTWPSPPHKGAGDGRDVFDGVALVRRDLLRVSDHVRHDP
                     QALAAEVLALCRGPAR"
     gene            6734..>7645
                     /gene="whiA"
                     /locus_tag="VFD74_00335"
     CDS             6734..>7645
                     /gene="whiA"
                     /locus_tag="VFD74_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HZK48039.1"
                     /translation="MSTVSFSRRVREELLRHSDARPCCRKAEVAALVRTSGTFHIKGG
                     ASEEEGRYALHLSTTSRAAARMVYSYFKEFGAEGRLMTRREPRFRRRLLYEVHLPGSP
                     PMLQALNELGILSDNFRLVPGLPSRILRSRCCKSAFVRGCMIGAGSVNLPSRSTHLEI
                     LSPHQDFAHDLARLLVSTGFAPGVYQRRGSHVVYLKSGEEVAAFFAFIGAQEAALKVE
                     EQAVMKDMRARANRLANCDEANTRRTSAAALRQLRAVDFLRKEGMLEHLPPALREVAE
                     LRSEHQFLSLAELAEEAPEGVSRSALNH"
BASE COUNT         1165 a         2458 c         2636 g         1386 t
ORIGIN      
        1 gtctcggcga acacgcggcc ggtgctggag tcggtcaccg gcatgtactt ctccgtcgcc
       61 gactcgcgcc actcgccatc gatgcagtat ttcagggtct gcactccggt cacagcttct
      121 gccatctcga tgctcctcct ccgtgtaggt gctagcttgt gaagtgtatc acgtgcacgc
      181 ggttgatccc gattacacgc ggttgatcac gaggtatgtt accactggtc tgaccacggg
      241 gcgagttgcc ctataggtgc ttgtgcgccg aaagcagcgc ctgcacgagc aagtccgcgc
      301 cctgatcgat gtctttcagg gacaggacct ctatgggtgc gtggatgtag cgggtggcga
      361 tgctcaccac gcccgacggg acgcccttct tggtgagatg gatggcggtg gcgtcggtgg
      421 tgccgccgct gcccacgtcg agctgatagg ggatcttggc cgaatctgcg gactctcgca
      481 gccagttgag gatcttcttg ggaacgatga cgcctcgccc gtccgaatcc acaacggtca
      541 gcaccggtcc cttacccgtc atcacgtgcc gctgctcctt ggtcacgccc ggatggtctc
      601 cgggtacggt gacgtcggcg atgatggcgg cgtcggggtc gaggctgaag gcgctagtac
      661 gtgcgccctt gaggccgacc tcctcttgga cggtgcccac cgcgtgcacg gtggccttgc
      721 acttcttgcc cttcagtcgc tgcatggcgg cgatcatcat gaccacgccc gcgcggttgt
      781 cgaaactctt gccggtgacg aacccgttgg ccagggtcgc catggtcctg tcgagcgtga
      841 ccggggaacc gatctctaca cccagggcgg cggcatcgtc ggcgtcgatg gctccgatgt
      901 cgatgaacat ctccttgatt ttcacgatct tcttgcgctc ttcgtcgtcc atgatgtggg
      961 gaggtttggc tccgatgact cccgtcaatg cgcccttcgt gccgtgcacc agcacgcgtt
     1021 gtcccaggac catctggtcg aaccatccac cgagagggac gaacctcaag aagccgctct
     1081 cgtcgatgta cttgaccatg aagccgatct cgtccatgtg ggcggcgatc atgatggacg
     1141 gccccgggcc ggtctttgtg ccgacgacgt tgcccatcgc atcggtgctc acttcgtcca
     1201 cgagcggttc gagttcggcg acgaggagcg atgcgacggc gccttcgtga ccggacactc
     1261 cgtgggcgtc ggcaaagagg cggagcaggc gttcgatgtc cttcatgcgc gttcctttcg
     1321 gaagacgggc tacagcgtcg agtgcggccc gcggtcgttc gttcgtcgtg agtgtaccgc
     1381 gtccgggtgc tgccgcgcgc gcccgtggta tcctccagtc ttcttacgca ttcttgggat
     1441 acacgtgttg ggcgccggtt gctgaggcgc cgggtcgaac gagggaggca tcgatgggcg
     1501 ggctgctcga gggtcgaagg ggcctcatca tgggcgtggc caatgagcgg agcatcgcat
     1561 ggggcatcgc gcaggcggcc aggcgcgagg gcgccgaact cgcgttcacg ttcgcggggg
     1621 agatactcga gaagcgcgtg cggccgctcg cggctcagtt gggctccgga ttcgtccttc
     1681 cgtgcgacgt ccaggtggat gaggacatcg agtctgtctt ctccgcgatc ggcgccgagt
     1741 gggggtcact cgacttcttc gtgcactcgg tggctttcgc gaaccgtgag gatctcaagg
     1801 ggtctttcgt cgatacaccg cgcgacggct tccgtctggc gctcgacgtc tcggtgtatt
     1861 cactggtcgc gtcggtgcgg gcggcactgc cgtacatgaa gccgggcggc agcatcctca
     1921 ccatgaccta cctgggcggc gaacgcacca tccctcacta caatgtgatg ggggttgcca
     1981 aggcggctct cgaatcggcg gtgcgctacc ttgctgcgga cctcggcccg cggggtattc
     2041 gcgtgaacgc gatctcggcc ggacccgtcc gcaccctggc ggccgccggc atcggtgact
     2101 ttcggaagat actcgattgg tatgagcaga acgccccgct ccgcaaagcg acgactcccg
     2161 acgaggtggg cgacgccgca ctcatgttgc tgggcgacct gggtcgggca acgacgggcg
     2221 aggtgatcca tgtcgacggt ggctaccacg ctgtgggagt acctcccgcc gtcgaggaac
     2281 acgacgaggg gtagctccga acacgacgtc gcggtccgag ccttcgggcc gcttggttga
     2341 ccatggacgt gtctgaactc gaacgtcaga tcaaggaagc tcccgatctg ccgggtgtct
     2401 acctcttccg tgacggggac tctgccgtcg tctacgtggg caaggccctg tcactacgcc
     2461 ggcgcttggc cagctatgcg cccgcggttc gagaagatgc ccatgtgccg gtgaaggtgg
     2521 gcgagatggc gcggcgggct gagtcggtgg agtggatcgt cacctcatcc gacgtcgagg
     2581 cccttctgct cgaacacaac ctgatcaaga gacaccgccc cgatttcaac atctgtctac
     2641 gcgacgacaa gtcgtacccc tacatcgtga tcacgatgga ggaggagttc ccgcgggtga
     2701 tgttcacccg gctgcctcac cggcgcggcg acctctactt cggcccgtac ggcagcgccg
     2761 ccaaggtgcg cgagacgctc gacgccctcg gccgcgtctt tccctaccgc aagtgccgag
     2821 gcatgactcc cggacgccgc aacggctcac cgtgtctgca gttccacatc aagcggtgca
     2881 atgcgccctg cgtcggaggt gtctcgcgcg aagagtatcg ggcggtggtc gcgcgggtcg
     2941 ccgacctgct ggccggtcgt catcttcagg cggtctcggc catggaggcc gagatgaggg
     3001 ttgccgcgaa cgcgcgcgac ttcgagaagg cggcgctgct gcgtgatcgt ctggaagcac
     3061 tgcggcacat cttggagcgc cagcaggtgc agggggcggt gcagggggcg gtggacataa
     3121 tcggtctcgc gcgggacgag ggcggcgcga acgtccaggt gttcctgacc cgggacgggc
     3181 tgctgtccga tcgccgcagt ttcacccttg tgggcactga aggcgcttcc gaggcggagt
     3241 tgttggagcg gttcatgggt gagtactact ccacggccgt atcggtgccg cccgagatag
     3301 tcgtgccgcg cacctcgtcc gaccttggca atctggctct gttcctcgag gggctccgcg
     3361 ggacgcgggt gaacgtgcgc cacgccgagc ggggcgacaa gcgtcggctg caggagttgg
     3421 ctgatcgcaa cgccgtgctg gccctggagc acgagcgcct gcgcagcgcg cgcagccgcg
     3481 agcggcgctt gggagggttg tcggcgctgc aggcctcgct gggcctcgac ggtccgccca
     3541 tccggatcga aggctacgac atctccaacc ttggatcgga acacatagtc gcttcgatgg
     3601 tggtcttcga agggggcgca gccaagaagt ccgactatcg caagttcgtc atccggggcc
     3661 tcagcggcca ggacgacttc ggggcaatcc acgaggctgt gctcaggcgg ttcacgcgga
     3721 agactggagt cgacaccccg gaggcgtacg atccgtcgtt cgaggccgtg cccgacttgg
     3781 tgctgatcga tggcggcaag ggccagctgt cggccgccct acaggcgctc gaggaggcgg
     3841 gcctcgacga cgtagtgccc gtggtctcgc tggccaagcg ggaggaggag ttgttcgtgc
     3901 ccggtcgcgg agcccctttg gacgtggcgc cggacgaccc gggcctcatg ctgctgcgga
     3961 gcgtgcgtga cgaagcccac cgcttcgccg tggggttcca ccgagcgcgg cggcaggcga
     4021 ctgcgacgga ttcgttcctc gacggacttc ctggggtggg cgagaaacgg aagcgtgcca
     4081 tccttgccca cttcgggtcg cccgaacgct tcctgcaggc caccagcgaa gaggtggaag
     4141 cggtgccggg attgcccggg agagtggcgc gattcatcta cacctatgtg cacaagaatg
     4201 ggtagactgg gaggcgtggt gagcgaagcg acattccgga cgccttgggg actgggccgt
     4261 gtcgtcgtcg agggcgaata cttggtcgag gtggtcctgc cgcggctcga cgctccgccc
     4321 gtcgttcagg gcgaggagcc tcccgacggc gcgatgacgg ctgacggtgg cgcgaccggg
     4381 gctgcggcgg cctcggggtc ggacgcagcc gtccgcgggg cgctgcccga cccgcccgat
     4441 ccggccgcgc gctgggcgcg cgagctggac gactatttca gctgcgggcg ccaagggtgg
     4501 agcgatgagg aagtccgtct ggaggagcgc cggttcactc ccttccgcac cgccgtgtat
     4561 agggcgctcc tggaagtgcc ggttggcagc accgtcacct acggggagct ggcccgccga
     4621 gtcggtcgcc ctggcgcagc gcgcgcggtc ggcaccgcga tggctcagaa cccgatcgcc
     4681 gtcgtcgtgc cgtgtcaccg ggtggtccgc aacgacggtt ccctgggaca ctatggcttc
     4741 ggagacgcat ggaaacccct cttgcttcag ctcgaaggcg cacgcgcatg acggctctca
     4801 aggatcttca cttcaccgtg atcacgggtc tgtcgggggc gggcaagtca caggctgtag
     4861 ccaccttcga ggacatgggt ttcttctgca tcgacaatct gcccccccag atgctcgccg
     4921 gcgtcttcga gttgttctcg ctggaaggga gcagggtcga ccgactggcg gtggtcttcg
     4981 acgtgcgcgc cgcccagtac ttcgacgaac tcatgcggtc tctggacgac ttgaccgagg
     5041 gcggggccca gtgccacatc ctgttcctcg aggcgtccga cgaggcgctg gtggcccgct
     5101 accagtcgac gcggaggcct caccccgttg ctcacgacgg gcctctcctc gcgggcatcc
     5161 aagaagagcg gcgcctgctc atgccccttc gtgagcgcgc cgacgtggtc atcgacacca
     5221 ccgagctgaa cgtccacgag ttgcgccacc gcctccagga gaccctcttc gcgggagaac
     5281 tgacgaacga gatcctggtg agcctcatct ccttcggatt caagttcggc ctcccggcgg
     5341 aggcggacat ggtgctcgac gtccgcttcc tgcccaaccc gcactgggtc gaggagctgc
     5401 ggccgctcac cggcctcgat agcgcggtcc gagatttcgt cgtggcgcga cccgagtccc
     5461 agggcttcat cgagagggcc gcggccctgc tgctctacct cgtgccgctc tacctggcgg
     5521 agcagaagac ccaactcgtg gtcgccatcg gttgcaccgg cggacggcat cgctcggtgg
     5581 ccctggccga agccctggcg gcatgtcttg ccaaggaggc tcacgttcag accgcagtga
     5641 ggcaccgaga tgtcgaacgt tcgggctgat cccgcggctg tggactcggc tcgggctgcg
     5701 tacgcgaacg atccggcgac ggaggcgcac catccgcggg tcgtggccgt gggcggaggc
     5761 acgggcctgt cgacgctcct gcgggggctg aagcacctca cgcccaacat cacggcggtc
     5821 gtcactgtcg ccgacgacgg gggctcttcg ggcaggctgc gggccgacct aggggtgccg
     5881 ccaccggggg acatcagaga ctgcctgctc gcgctggccg atcacgaatc cctgctcgca
     5941 tcgctgttct cccatcgttt cgcctcggga gagctggcgg gccacagcgt gggcaacctc
     6001 ttactggcag ctctcacgga ggtcttggga gacttcgaca aggcggtcaa ggagtcgagc
     6061 aaggtcctgg ccatcaccgg catggtgctc ccgggcacca cctccgacgt gcggcttcac
     6121 gcgcttctga gcgacggtcg ccatctcgtg ggggagtcac gcatcgggag gtcgccggtt
     6181 tccgtgagca gggtgtggct ggaacccgaa gacgcgcagg ctctgccact cgcgctcgcg
     6241 cgtatcggcg aggcggacct ggtggtgctg gggccgggca gcctcttcac cagcgtgatg
     6301 cccaacctgc tgttgggagg gatgcgcgag gctcttgcgt cgaccgaggc gcgtgtggtc
     6361 tacgtggcca acgtcatgac acagccggga gagaccgctt cgttcaccgg tcgcgatcat
     6421 ctggcagcct tgatcgatca tcttggccaa ggcgtcctgg acgcggtgtt ggtgaactcg
     6481 acccccgtgc ccgacggcgc tctcgaacgg tacggctgcg cgggtgcgga gccggtcacc
     6541 tggccgtcgc cgccccacaa aggggcgggc gatgggcgcg acgtgtttga cggggtggcg
     6601 ctcgtgcgcc gcgaccttct gagagtctcc gaccacgtgc gccacgaccc tcaggcgctg
     6661 gcggctgagg tgcttgccct gtgccgggga ccggcgagat gaatacggcc gccctccgcc
     6721 tggaggccgt ctcatgagca ccgtcagctt ctcgaggcgg gtgcgggagg agttgctgcg
     6781 tcacagtgac gcgcggccct gctgtcgcaa ggccgaggtg gccgctctcg tacgcacctc
     6841 gggcaccttc cacatcaagg gcggggcttc ggaggaagag gggcggtacg ccctgcatct
     6901 ctccacgacc tcgcgtgccg cggcgcgcat ggtctactca tacttcaagg agttcggcgc
     6961 cgaaggccgg ttgatgaccc gcagggagcc gcgattccgt cgccggctcc tctacgaggt
     7021 gcacctgccg ggcagtccgc cgatgctgca ggcgctgaac gagctgggga tcctcagcga
     7081 caacttccgc ctcgtgcccg gcctgccctc aaggattctg cgcagtcggt gctgcaagag
     7141 tgccttcgtt cgcggctgca tgatcggggc ggggtcggtg aaccttccct cccgcagcac
     7201 acacctggag atcctgagcc cgcaccagga cttcgcgcac gatctggcgc gcctgctcgt
     7261 ctcgaccggc ttcgcccccg gtgtctacca acgccgaggc tctcacgtgg tctacctcaa
     7321 gagcggggag gaggtggcgg cgttcttcgc attcattgga gcgcaggagg cggctctcaa
     7381 ggtggaggaa caggcggtga tgaaggacat gcgcgcccgt gccaaccggc tcgccaactg
     7441 cgacgaagcc aatacccgtc gcacgagcgc cgccgctctc aggcagctgc gggcggtcga
     7501 cttcctgcgg aaggagggga tgctcgaaca cctcccgccc gcgctccgcg aggtggcgga
     7561 gcttcgctcg gagcaccagt tcctcagtct cgccgaactc gccgaggagg cccccgaggg
     7621 cgtgagtcgc tcagcgctca accat
//
LOCUS       DASRMJ010000015         3596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_398744, whole genome shotgun sequence.
ACCESSION   DASRMJ010000015 DASRMJ010000000
VERSION     DASRMJ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398744"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VFD74_00340"
     CDS             complement(<1..965)
                     /locus_tag="VFD74_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK48040.1"
                     /translation="MYLKHLLVEGFKSFPEPSGLELHPGVCVFVGANGTGKSNLTDAL
                     TWALGENDMGDLRCRSEADLVFAGSDELLPLDSSEVTIVLDHRPERLELQGLPPEVCK
                     HGHASNHTRDVPEGTLTITRRARRGENTHYLLDGAEASQADVAARLADLGIGPAHVSA
                     IRQGELDRLLLLDPRSRRRLIEEAVGIPELSLRHESLRSERSIVSTRHEHLLAGREAA
                     QERVADLEREARALDRLRALEAEQASLRAEVVRRALSAGAEAGSAEVPPVAELLALLG
                     LPASDPEPARATGGEAAPERAPGADGAPTTAATGWDELCSGLGVCA"
     gene            complement(1008..1925)
                     /locus_tag="VFD74_00345"
     CDS             complement(1008..1925)
                     /locus_tag="VFD74_00345"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZK48041.1"
                     /translation="MELAQLRYFTAVADTLSFTEAAARLHVSQPALSYQIKHLENELG
                     ARLFDRTSRRVALTVDGRAFLPLAQAVLLKTEEAARVMEERLGVITGSVRFGCIPSVA
                     AHVVPPILASFARNWPGIRVTLLEAGASDVERSVLDGSADFAIVPDPHAPELLEVTPL
                     IEEDLLVVLPHLHPLAEREAISLRELANEPLIILGGAFTLGPYVVELCRRAGFEPQVA
                     YETGALESVKGFVRNGLGIAVLPKMALQPADEDTMTVVPFVERLTRRLNLIRSKDRYA
                     NVASRTLMVHVRAAILSSGGSLGKPLKGS"
     gene            2295..3017
                     /locus_tag="VFD74_00350"
     CDS             2295..3017
                     /locus_tag="VFD74_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48042.1"
                     /translation="MAMYVELFGRDKYYGGMLRESREDEFVSNVFGPLRNFETTTWLV
                     ELLGKAFRGGDYGKAIESGYELDFWRRFPPPGGREVPENETEVSCVLETETFTFLVIA
                     RYWHELSRQTAHDVDRDQIVRTLDAGYAALGDRCRLLVLTTDAEPPALVSKYWADPAL
                     LAARMTGLPEGVDPQGLAARIGWTNWGELREALASRIGETPINLTQWNFAKDLVAYLD
                     LVSTRGRGLIVRATHGPADSGS"
     gene            3181..>3596
                     /locus_tag="VFD74_00355"
     CDS             3181..>3596
                     /locus_tag="VFD74_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48043.1"
                     /translation="MWLLVVAAVLLVGGAGLAARAAFGGGSVGAPSATATTVAAGDGG
                     SAGADGASGSGPGDGGTATPTTAVTAPSGAAPADDGPIGPPAAVSPINTSFAGLAMFR
                     GNSHRSYYGTGPVPTAPRVLWRFPESGPMSASSSEGG"
BASE COUNT          536 a         1225 c         1251 g          584 t
ORIGIN      
        1 gcgcagactc ccagtcccga acagagttcg tcccagcccg tggccgctgt ggtcggcgcg
       61 ccgtcggcgc ctggggcgcg ctcgggcgcg gcttccccac cggtcgcgcg cgcgggctcc
      121 gggtcggagg cgggcagccc cagaagggcc aagagttccg ccacgggtgg cacctccgcg
      181 cttccggcct ccgcacccgc cgagagcgct cgcctgacga cctcggctcg gagcgaggcc
      241 tgctctgcct ccagcgcacg caggcgatcg agcgcccgcg cctcacgttc gaggtcggcg
      301 acgcgttcct gcgccgcctc acgtccggcc agcaggtgtt cgtgccgcgt cgagacgatg
      361 gaccgttcgg agcggaggct ttcgtgacgc aggctcaact ccggtatgcc caccgcttct
      421 tcgatgagcc gtcggcgcga gcgggggtcg agcagtagaa gacgatccaa ctctccctgg
      481 cggatcgccg agacgtgagc cgggccgatt cccaagtcgg cgagtcgcgc ggccacgtcg
      541 gcctgggacg cttcggctcc gtcgaggagg tagtgggtat tctcgccgcg gcgcgcgcgc
      601 cgggtgatgg tgagcgtgcc ttcggggacg tcgcgggtgt ggttcgaggc gtggccgtgc
      661 ttgcacacct ccggagggag tccttggagt tcgagccgct ccggccggtg gtcgagcact
      721 atggtgactt cgctcgaatc gagcgggagg agttcgtcgc tgcccgcgaa caccaggtcg
      781 gcttcggagc ggcagcgcag atcgcccatg tcgttctcgc ccagcgccca ggtgagggcg
      841 tcggtgaggt tgctcttccc cgttccgttc gcgcccacga acacacagac gcccggatgg
      901 agctcaaggc ccgacggttc gggaaagctc ttgaatccct ctaccagcag gtgtttcaga
      961 tacacgggtc tcatcactcc tagcggcgct ccgtcacggg acggattcta gcttcccttc
     1021 aggggtttac caagtgagcc cccgctggag aggatcgccg cccgcacgtg caccatcagc
     1081 gtgcgggagg ccacgttggc gtagcggtcc ttgctccgga tgaggttgag tctgcgcgtg
     1141 agtctctcga cgaagggcac gaccgtcatg gtgtcctcgt ccgccggttg cagagccatc
     1201 ttggggagaa cggcgatacc caggccgttc cgcacgaagc ctttgaccga ctccagggcg
     1261 ccggtctcgt aggccacctg cggctcgaaa cccgcgcgcc ggcagagttc gaccacgtag
     1321 ggccccaagg tgaaggcccc tccgaggatg atgagtggtt cgttcgccag ttcgcgcaac
     1381 gagatggcct ctcgttccgc gagcgggtgg aggtggggaa gcacgaccag caggtcttcc
     1441 tcgatgagtg gggtcacctc cagcagctcg ggcgcgtggg gatcggggac gatcgcgaag
     1501 tccgccgaac catcgagtac gctgcgctcg acgtcgctcg ccccggcttc gaggagggtg
     1561 accctgatgc cgggccagtt gcgcgcgaag gaggcgagga tcgggggcac cacatgtgcc
     1621 gccacgcttg ggatgcagcc gaaccgcacg ctgccggtga tcacgccgag gcgttcctcc
     1681 atgacgcgcg ccgcctcttc ggtcttgagc aggacggcct gagccagggg taggaaggcg
     1741 cgtccgtcca cggtgagggc gaccctcctg ctggtgcggt cgaacagccg ggctccgagc
     1801 tcgttctcga ggtgcttgat ctggtagctc agggccggct gagacacatg gagtctggcc
     1861 gccgcctcgg tgaagctcag cgtgtcggcc accgccgtga agtatctcaa ttgggcgagt
     1921 tccataaata gaaattatat caagcataga aacatgatat tggataaatc gttgggccgc
     1981 ggctacacta ccgtcagctg agcactgaac aggcgtggat caccgggatc ccccccattc
     2041 cgccgcgccc agtgttgagc agttttcccc gctgtatagc cggtcctacc ccctgggacc
     2101 ggctatacgt ccatttgaag ccggtctgac tgcggccggc tggtgtgtgc gacatgcccc
     2161 gctcctatgc ctgtcgcccc cgtccgcggt ccctccgcca cttgccaggg gggcaccgga
     2221 acgctagcat cgcaccagca cgggaaggcg gggtccgcgc ggcttcgcct cacgcataga
     2281 agggggcctg gggtatggcg atgtatgtgg aactgttcgg tcgcgacaag tactacggcg
     2341 gcatgctgcg tgagagtcgc gaggacgagt tcgtatcgaa cgtcttcggt cccctgcgca
     2401 acttcgagac gaccacctgg ctcgtggagc tcctggggaa ggcctttcgc gggggggact
     2461 acgggaaggc gatcgagagc gggtacgaac tcgacttctg gcgacgtttc ccgcccccgg
     2521 gcgggcgtga ggtccccgag aacgagaccg aggtctcgtg cgtgctggag acagagacct
     2581 tcacgttcct ggtgatcgcg cgctactggc acgagctcag ccgccagacc gcgcacgacg
     2641 tggatcgaga ccagatcgtc cgcaccctcg acgccggcta cgccgcgctc ggagatcgct
     2701 gccgcctgct ggtgctcacc accgacgctg agccacccgc tctcgtgtcg aaatactggg
     2761 ccgatccggc gttgctggcg gcccggatga ccggtctccc cgagggcgtc gacccgcagg
     2821 ggctcgccgc ccgcatcggc tggaccaact ggggcgagtt gcgcgaggca ttggccagtc
     2881 ggatcgggga gactcccatc aatctcaccc agtggaactt cgccaaggac ctggtggcgt
     2941 acctcgacct ggtctccacg cgcggccgcg ggctcatcgt ccgggccacc cacggcccgg
     3001 ccgacagcgg gtcctgaggg ggcacgcacg cggcctcgcg aagacgcacg cgggcggggg
     3061 agcgacgccc gcccccacga ggggccgccg cgcggcggcg ccgccccgaa gggagacgca
     3121 cgcccctacc acgtgtaccc gtcacgcccg ggtagaggtc ccacgcgcag ccggttcccc
     3181 ttgtggctcc ttgttgtggc ggccgtgctg ctggtgggag gagcggggct cgcggcccgc
     3241 gctgcattcg ggggcggctc cgtcggcgcc ccctctgcca ctgcgaccac cgtggcggcg
     3301 ggcgacggtg gcagcgcggg cgccgacggc gcttccggct ctgggccggg cgatggaggg
     3361 accgccacac ccacgaccgc ggtcaccgcg ccctccggcg ccgcacccgc ggacgacgga
     3421 cctatcgggc cgccggccgc cgtctcaccc ataaacacct cgttcgcggg cctcgcgatg
     3481 ttccggggta actcccatcg gtcgtactac ggcacggggc ccgtccccac ggcgccacgc
     3541 gtcctctggc gcttccccga gagcggcccg atgagcgcat cttcgtcgga gggggg
//
LOCUS       DASRMJ010000016         4168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_435285, whole genome shotgun sequence.
ACCESSION   DASRMJ010000016 DASRMJ010000000
VERSION     DASRMJ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435285"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFD74_00360"
     CDS             <1..448
                     /locus_tag="VFD74_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HZK48044.1"
                     /translation="RLIALIDKGEPLPVDVAGQIIYYVGPSPAKPGRPIGSAGPTTSG
                     RMDAYTPVLLDMGLKGMIGKGYRNPAVKDAMIRNKAVYFASVGGAAALIAQRITAARV
                     VAYDDLGPEALREIDVVDFPVIVINDIYGGDLYEAGRREFERKVGT"
     gene            451..843
                     /gene="sdhC"
                     /locus_tag="VFD74_00365"
     CDS             451..843
                     /gene="sdhC"
                     /locus_tag="VFD74_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZK48045.1"
                     /translation="MKDRETANVGAGLWKGAGMWAWLFHRVSGVVLALYIFAHIWVIS
                     RAAASGADAFDSMFKTLESPFFVLLDLALLAAVVYHALNGIRVILFDLGIAIRRQKTL
                     WWVVIAVSVLVMEVFVFVSFSYIASHGS"
     gene            860..1222
                     /locus_tag="VFD74_00370"
     CDS             860..1222
                     /locus_tag="VFD74_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZK48046.1"
                     /translation="MRKETLELSTGGMWLWLLQRVTAVLLLLFLTAHLIATHVLNLGE
                     LSYDNVGGRLASGFFVVVDFGLLGAGLFHGLNGLRMVLLDYGFGGRSQSVLSWILLVV
                     GLAAFAGGTWALWPWLTT"
     gene            1239..2951
                     /locus_tag="VFD74_00375"
     CDS             1239..2951
                     /locus_tag="VFD74_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK48047.1"
                     /translation="MIYHELIVVGGGLAGLRAAVEAKKQGIDVAVISQVWPGRSHSGA
                     AQGGVNAALANHPDSADDTWEKHAFDTVKGSDYLADQNAVAQMTKDAIPIIYEMEHWG
                     CPFSRFDDGRIAQRPFGGAGYPRTCYGADKTGHYMLHTLVERAAAAGVTMYVEWFVNR
                     LVVDDGVCRGVVAYDMMKGGFEFFQGDAVIVATGGAGRTYGNTTNAIISTGYGMAMAY
                     HAGVPLKDLEFVQFHPTGLLTTNILMTEGARGEGGYLVNGLGERFMKKYAPTAMELAP
                     RDITSRSIQTEINEGRGIDGKGFVYLDLRHLGAQKILEQLPGIRDLAIHFEGVDPIEE
                     PIPIVPSQHYSMGGIDTDMDGRTIMPGLYAAGECACVSVHGANRLGGNSLLETVVFGR
                     RAGAAATEHLGGLAAKKGSAAAGEAAVIAARAQVDDLASRAGSAGAEDPYAIRLGMTT
                     TMKDHFGVFREASVMQAGLDKMIDLKQRVRNIGLRHTGGVFNLDMIRTVELEGMVDVA
                     LAIAAGALARTESRGSHSRTDFPDRDDANWLRHTLAFNTPDGPSLESKPVALGMFEPQ
                     ERKY"
     gene            2951..3688
                     /locus_tag="VFD74_00380"
     CDS             2951..3688
                     /locus_tag="VFD74_00380"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZK48048.1"
                     /translation="MPEAKFRIKRYDPAQSADQNGAAGRRYDEFRVPYESSLTVLEGL
                     FYIQENLDGSLSFRYCCRAAVCGSCAMYINGAYRLACQTNVRHLGDGVITVEPLPHMP
                     LVKDLVCDMTEFFEKYEAIKPYLMRDSVEPEKEIAQSPHERKKLDGPIDCILCGSCYS
                     SCPSVWGEGNYLGPAALLKAYRFEVDSRDEAKKARLPSFDNERGTYRCHTITNCVEAC
                     PKELNPTEGIQWLKGAAVRRRILGRAK"
     gene            complement(3814..>4168)
                     /locus_tag="VFD74_00385"
     CDS             complement(3814..>4168)
                     /locus_tag="VFD74_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HZK48049.1"
                     /translation="NCVQVFRTGEGLQRTVTILSDLRTRYDGTAVRAGGRVFNLDLLR
                     VLELDGMIDLALATAMGALARQESRGAHSRADFPLRDDANWLRHTLAYHTDQGPRLGY
                     KPVVVDQFQPQDRTY"
BASE COUNT          694 a         1369 c         1409 g          696 t
ORIGIN      
        1 gcggttgatc gcgctcatcg acaagggcga gccgctgccg gtggacgtcg ccgggcagat
       61 catctactac gtgggccctt cgccggcgaa gccgggcagg ccgatcggtt cggccgggcc
      121 caccacttcc ggccgcatgg acgcctacac tcccgtcttg ctcgacatgg ggctcaaggg
      181 catgatcggg aaggggtatc gcaacccggc cgtcaaggac gccatgatcc gcaacaaggc
      241 cgtgtacttc gcttctgtgg ggggggccgc cgccttgatc gcgcagcgca tcacggcggc
      301 gcgcgtcgtg gcctacgacg acctgggtcc ggaagctctc cgggagatcg atgtggtcga
      361 tttcccggtc atcgtgatca acgacatcta cggcggcgac ctctatgagg cgggccgccg
      421 ggagttcgaa aggaaggtgg gcacttgagc gtgaaggacc gagagacggc caacgtgggg
      481 gccgggctct ggaagggcgc cggcatgtgg gcctggctct tccatcgggt gagcggtgtg
      541 gtgctcgccc tctacatctt cgcccacatc tgggtcatct cccgagcggc ggcctccggc
      601 gccgatgcgt tcgactccat gttcaagacg ctggagagtc cgttcttcgt cctgctcgat
      661 ctcgccctgc tcgcggcggt cgtgtatcac gcactgaacg gcatccgcgt gatcctcttc
      721 gatctgggca tcgcgatccg acggcagaag acgctgtggt gggtagtgat cgcagtctcc
      781 gtgctggtca tggaggtctt tgttttcgtc tcgttctcat acatcgcctc gcatggctcg
      841 tgacagggga gtcgctgacg tgagaaaaga aacactcgag ctcagcaccg ggggcatgtg
      901 gctgtggctt ctgcagcggg tgacagccgt ccttctgttg ctgttcctga ccgcgcatct
      961 catcgccacc cacgtgctca acctcggcga gctctcgtac gacaacgtag ggggcagatt
     1021 ggcttcgggc ttcttcgtgg tcgtggactt cggcctgctc ggagcgggcc tcttccacgg
     1081 gctcaatggg ctccgcatgg tgctcctcga ctatgggttc ggcgggcgca gtcagtccgt
     1141 gctctcgtgg atcctgctgg tggtgggatt ggccgccttc gcaggaggca cctgggcgct
     1201 ctggccgtgg ctgacgacgt agggaagggg tccgcagcat gatctatcac gagctgatcg
     1261 tcgtcggagg gggcctggcg ggccttcgcg cggcggtgga agccaagaag cagggcatcg
     1321 acgtggccgt catctctcag gtgtggccgg gccgcagcca ctcgggggcc gcacagggcg
     1381 gggtgaacgc cgcgctggcc aatcaccccg attcggcgga cgacacctgg gagaagcacg
     1441 cgttcgacac ggtgaagggc tccgactacc tggccgacca gaacgccgtc gcccagatga
     1501 ctaaggacgc catcccgatc atctatgaga tggaacactg gggatgtccg ttcagccgct
     1561 tcgacgacgg ccgcatcgcg cagcgcccgt tcggcggcgc gggctacccg cgcacctgct
     1621 acggcgcgga caagaccggc cactacatgt tgcatacgct ggtcgagcgc gccgcggccg
     1681 caggcgtgac catgtacgtg gagtggttcg tcaaccgcct cgtcgtggac gatggagtct
     1741 gccggggcgt ggtcgcctac gacatgatga agggcggctt cgagttcttc cagggtgacg
     1801 cggtgatcgt cgccaccggc ggcgcaggac gcacctacgg caacaccacc aacgccatca
     1861 tctccaccgg ctacggcatg gccatggcct accacgcggg cgtgcccttg aaggacctcg
     1921 agttcgtcca gttccacccg acgggcctgc tcaccaccaa catcctcatg accgagggtg
     1981 ctcggggcga gggcggctat ctcgtcaacg gcttgggcga acgtttcatg aagaagtacg
     2041 cgcccacggc gatggagctc gctccccgcg atatcacgtc ccgctcgatc cagaccgaga
     2101 tcaacgaggg ccgcggtatc gacggcaagg gcttcgtgta cctcgaccta cggcatcttg
     2161 gcgcacagaa gatcctcgag cagctgccgg ggatccgcga cctggccata cacttcgagg
     2221 gggtcgaccc catcgaggag cccataccca tcgtgcccag ccagcactac tcgatgggcg
     2281 gcatcgacac cgacatggac ggccgcacca tcatgcccgg cttgtacgcg gccggcgagt
     2341 gcgcctgcgt gtccgtgcac ggcgccaacc ggctcggcgg caactcactt ctcgagaccg
     2401 tcgtgttcgg ccgtcgggcc ggcgccgccg ctacggagca tctgggcggt ctcgcggcga
     2461 agaaggggag cgccgcggcc ggcgaagccg ctgtgattgc cgcgcgcgcc caggtggacg
     2521 accttgcctc gcgcgccggg agcgcgggcg ccgaggaccc gtacgccatc cgtctcggca
     2581 tgaccaccac catgaaggac cacttcggcg tgttccgcga ggcctccgtg atgcaggcgg
     2641 gtctcgacaa gatgatagac ctcaagcagc gcgtccggaa catcggcctg cgccacaccg
     2701 gcggagtgtt caaccttgac atgatccgca cggtggagct cgaaggcatg gtggacgtgg
     2761 ctctggccat cgcggccgga gcgctcgcgc gcacggagtc ccgcggctcg cactcgcgca
     2821 ccgactttcc ggaccgcgac gacgcgaact ggctccgtca caccctcgcc ttcaatacgc
     2881 ccgacgggcc gagtctcgaa agcaagcccg tcgccctcgg gatgttcgaa ccacaggagc
     2941 ggaagtactg atgccagaag ccaagttccg catcaagcgc tacgaccccg cgcagagcgc
     3001 ggaccagaac ggcgccgccg gccgtcgata cgacgagttc agagtgccgt acgagtcgag
     3061 cctcactgtg ctcgaaggcc tcttctacat ccaggagaat ctggacggtt cgttgtcgtt
     3121 caggtactgc tgtcgggcgg ccgtgtgcgg gtcgtgcgcc atgtacatca acggcgccta
     3181 ccggctggcc tgccagacga acgtgcggca cctcggtgac ggagtcatca cggtggagcc
     3241 gctgccccac atgccgctgg tcaaagacct ggtgtgcgac atgaccgagt tcttcgagaa
     3301 gtacgaggcc atcaagccgt atctcatgcg ggactccgtg gagccggaga aggagatagc
     3361 ccagtcaccg catgagcgga agaagctcga cggcccgatc gactgcatcc tctgcggcag
     3421 ctgctacagc tcgtgcccgt cggtgtgggg cgaggggaac taccttggtc ccgcggctct
     3481 gctcaaggcg taccggttcg aggtggactc gcgtgacgag gccaagaagg cgcgcctccc
     3541 cagcttcgac aacgagcgcg gcacctaccg gtgccacacc atcacgaact gcgtggaagc
     3601 ctgcccgaag gagctcaatc ccaccgaggg catccaatgg ctcaaggggg cggcggtgcg
     3661 gcggcgcatc ctggggcgcg cgaagtagct cgccggctgg gctgtgcggc tcccgcgccg
     3721 ggcgccgcac gcggagccgc gccgagagcc gtgcccggag tccgacgccg tcgtccggcg
     3781 gcgcgagggt cgcggccggg cgggaccgct cagctagtac gtgcggtcct gcggctggaa
     3841 ctggtcgacc accaccggct tgtagcccaa gcgcggaccc tggtcggtgt ggtaggccag
     3901 ggtgtgacgg agccagttcg cgtcgtcgcg gagggggaag tccgcacgag agtgcgcccc
     3961 gcgtgactcc tgccgggcga gcgcgcccat ggcggtcgcc agagccaagt cgatcatgcc
     4021 atcgagttcc agcaccctca gcaggtctag gttgaagact cggccgccgg cacgcacggc
     4081 cgtcccgtcg taccgggtcc tcaggtcgct cagtatggtg accgtacgct gcaacccctc
     4141 gcccgtgcgg aacacctgca cacaattg
//
LOCUS       DASRMJ010000017         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_435343, whole genome shotgun sequence.
ACCESSION   DASRMJ010000017 DASRMJ010000000
VERSION     DASRMJ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435343"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFD74_00390"
     CDS             complement(<1..361)
                     /locus_tag="VFD74_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZK48050.1"
                     /translation="MDDARFEELVAEALDSLPDEFLAHLENVEVVIEEWPSEEELADA
                     GLEGEDAASLLGLYFGIPLTERGGDYAGVLPDRITLYQGSILAAAGEDPAAIREEVCV
                     TVVHEVAHFYGISDDRLE"
     gene            complement(354..1007)
                     /gene="rdgB"
                     /locus_tag="VFD74_00395"
     CDS             complement(354..1007)
                     /gene="rdgB"
                     /locus_tag="VFD74_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZK48051.1"
                     /translation="MTDSLSSPAADGGCARVVVATRNQGKAREFARLLGRGFSVETLP
                     AGVGLPEETGATFAENALLKALGAYVSLGAKVAVLADDSGLEVDALAGEPGVRSARYA
                     GDDATDEQNVALLLERLGGTAERAGRFVCALALVTPSRTGGAPQLHEVTGVLEGRIAA
                     APAGGEGFGYDPVFVPEGWDLTLGEGTAFQKDAVSHRARAVASLLDLLEREGVRIDG"
     gene            complement(1004..1747)
                     /gene="rph"
                     /locus_tag="VFD74_00400"
     CDS             complement(1004..1747)
                     /gene="rph"
                     /locus_tag="VFD74_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZK48052.1"
                     /translation="MCAVQSGTGRPPESLRQLEVTPDFVRSADGSVLFELGGTRLLCT
                     ASIVNGVPRRLRGTGEGWLTAEYSLLPASTGQRTSREAVSGRQGGRTVEIQRLIGRSL
                     RAVTDLSGLGERTVYVDCDVIEADGGTRCAAISGGYLALHLALKRAVDAKIIKSYPLH
                     DSVAAVSVGIVDGLPVLDLDYAQDSTADVDMNVVMTGSGRLIEVQATAEKVPFDREGL
                     DRLLDLAAAGIREIGAVQAAVIARSYSQE"
     gene            complement(1827..2714)
                     /gene="murI"
                     /locus_tag="VFD74_00405"
     CDS             complement(1827..2714)
                     /gene="murI"
                     /locus_tag="VFD74_00405"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZK48053.1"
                     /translation="MEERDATSRSGGNDRDPEDSSVDTRPVGLFDSGVGGLTVLHECL
                     VGLPSEDYVYLGDTGAFPYGPKSLEEVRALAFRNAAFLVEQDVKLIIVACNSAASAAL
                     PQLQEAFETPVVGVVMPGARAAVQETRNRRVGIMATEATVRSGSYQQALRSLDAGLDI
                     FPVACSRLAPMIQDGDVFSEEVEEAVRACVRPIKEAGADTVILGCTHFPLISPMLRRL
                     LGPRVSLINSAEEIAREVGEILERKGIGNLPGREGAYSFYATGDPEAFRRVGARFLQL
                     PIREVHLWDAGVVTDRQAG"
     gene            complement(2726..>2935)
                     /locus_tag="VFD74_00410"
     CDS             complement(2726..>2935)
                     /locus_tag="VFD74_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48054.1"
                     /translation="VCPVSISHVISRTLPRRLAPPPRPRMAADMGAAATAHAAAVGNA
                     ARAVTGFGSDARADAYPARPSVRVW"
BASE COUNT          480 a         1041 c          963 g          451 t
ORIGIN      
        1 cttcgaggcg gtcgtccgag atcccgtaga agtgcgcgac ttcgtgcacc acggtgacgc
       61 acacttcctc cctgatcgca gctgggtctt cgccggcggc ggccaagatc gagccttggt
      121 agagagtgat gcggtcgggg agcacgcccg cgtaatcgcc gccgcgttcc gtcagcggga
      181 tcccgaagta gagtccgagc agcgacgcgg cgtcctcacc ctccaggccg gcgtcggcca
      241 actcctcctc tgagggccac tcctcgatca cgacctcgac attctcgaga tgcgcaagga
      301 actcgtccgg cagagaatcc aaggcctctg cgaccaactc ctcgaaccgt gcgtcatcca
      361 tcgatccgca ccccctcgcg ctcgagtaga tccaacaatg aggccaccgc acgcgctcgg
      421 tgcgagacgg cgtccttctg gaaggcggtg ccttctccca aggtgaggtc ccaaccctcg
      481 ggcacgaaca ccgggtcgta cccgaaccct tcccctcccg cgggggccgc agcgatgcgg
      541 ccctcgagca cacccgtcac ctcgtgcagt tgcggcgcac cgcccgtgcg tgagggcgtc
      601 accaaggcga gcgcgcagac gaaccggccg gcgcgctcgg ccgtgcctcc aagccgttcc
      661 agcagcagcg ccacgttctg ctcgtcagtg gcatcgtcgc ccgcgtagcg cgccgatcgg
      721 accccgggtt cacccgcaag ggcgtccacc tccaggccgg agtcgtcggc caagacggcg
      781 accttcgcgc ccaacgagac gtaggctccc agggccttga ggagggcgtt ctcggcgaag
      841 gtcgcgcccg tctcctcggg gagtcccact cccgccggaa gagtctccac gctgaacccc
      901 cggcccaata gccgcgcgaa ctcccgcgcc ttgccttgat tgcgggtggc gaccaccacg
      961 cgcgcgcagc cgccgtcggc ggcgggcgat gagagagagt ccgtcactcc tgcgaatagc
     1021 tgcgcgcgat cactgcagcc tggaccgcgc cgatctccct gatccccgcg gctgccaggt
     1081 cgagcagccg gtcgaggccc tcacggtcga agggaacctt ctcagctgtg gcctgcacct
     1141 cgatcaagcg tccagacccg gtcatcacca cgttcatgtc gacgtcggcc gtggagtctt
     1201 gcgcgtagtc gaggtcgaga accggcagac cgtccacgat ccccacgctc acggcggcca
     1261 cggagtcgtg aagcgggtag ctcttgatga tcttggcgtc caccgcccgc ttcagcgcga
     1321 ggtgcagcgc gaggtagccg ccggagatgg cggcgcagcg tgttccgccg tcagcctcga
     1381 tcacgtcaca gtccacgtag accgtgcgct cgcccaagcc ggacagatcg gtcacggcgc
     1441 gcagactgcg tcctatcaga cgttggatct cgaccgtgcg gccgccctgg cggcctgaca
     1501 ccgcttcgcg cgacgtgcgc tggccggtgg acgcgggcag gagcgagtac tcggctgtca
     1561 accagccctc tcccgtcccc ctcagtcgac gcgggactcc gttgacgatg ctcgccgtgc
     1621 agaggagccg ggtgccgccc agctcgaaga gcaccgatcc gtcggccgac cgcacgaagt
     1681 caggagtgac ctccagctgt ctcaagctct cgggtgggcg gcccgttccg ctttgaactg
     1741 cgcacattct tagtatcctt tctgtcggcc gacggccgga tcgagccggt cggcctgtgc
     1801 cggcgaggct gacatgatgg agcgggtcaa cccgcctgac ggtcggtcac gactcccgca
     1861 tcccacaggt ggacctctcg tattgggagc tgcaggaagc gcgccccgac ccttcgaaac
     1921 gcttccgggt ctccggtggc gtagaagctg tatgcgccct cgcgaccggg taggttcccg
     1981 atacccttgc gttccaggat ctcccccacc tcgcgggcga tctcctccgc ggagttgatg
     2041 agcgacaccc gggggcccag caggcggcgc agcatcggcg atatcagcgg gaagtgcgtg
     2101 catccaagga tcacggtgtc ggctcccgcc tccttgatcg ggcggacgca ggcgcgcacc
     2161 gcctcctcca cctcctcgga gaacacgtcg ccgtcctgga tcatcggagc caatcgggag
     2221 caggccaccg ggaagatatc gagtcccgcg tcgagggatc ggagcgcctg ctgatagctg
     2281 ccggagcgca ctgtcgcctc agtggccata atcccgaccc tccggttgcg ggtctcctgc
     2341 acagccgcgc gagcgccggg catcaccacc cccacaacgg gcgtctcgaa ggcctcttgg
     2401 agctggggaa gagcggcgga ggcggccgag ttgcaggcga cgatgatgag cttcacgtcc
     2461 tgctccacaa ggaaggccgc gttgcggaag gcgagggcgc ggacctcttc cagcgactta
     2521 ggaccgtacg ggaacgcgcc cgtgtcaccg agatacacgt agtcttccga cggcaatccg
     2581 accaagcact cgtgcaggac ggtcaggccc cccacccccg agtcgaacag acccaccggg
     2641 cgggtatcga ccgaggagtc ctcggggtcg cggtcgttgc cgccggaccg agacgttgcg
     2701 tcgcgctctt ccatggggcc ggattctacc agacgcggac cgacggccga gccggatacg
     2761 cgtcggcacg cgcatcagag ccgaagccgg tgacggcgcg ggccgcgttg ccgacggcgg
     2821 cggcgtgggc ggtggcggcg gcgcccatat cggcggccat gcgcgggcgc gggggtggcg
     2881 caagccttcg tggtagagtt cggctgatga cgtgcgagat cgacacagga catac
//
LOCUS       DASRMJ010000018         5443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_218323, whole genome shotgun sequence.
ACCESSION   DASRMJ010000018 DASRMJ010000000
VERSION     DASRMJ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218323"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFD74_00415"
     CDS             complement(<1..255)
                     /locus_tag="VFD74_00415"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZK48055.1"
                     /translation="MYPQLLTIGPLTIHSFGVMMALAFVSAGLVAAWGMKRRGVDPEL
                     AYSMLIAAIVGGVVGAKLHYVIAHPDQARTALFSGSGLIWY"
     gene            complement(331..987)
                     /locus_tag="VFD74_00420"
     CDS             complement(331..987)
                     /locus_tag="VFD74_00420"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK48056.1"
                     /translation="MNRKYLIAIIVVAIVVVGLIVAIPNLVGSADGTTDTTVAAPPIS
                     PDAAVPDGHPSTGDTGTGPTAEELVKTAEEAYKADPKNVDLILALAEAYLQANNPDEA
                     TRLLDEAAALEPDNPKVKSGFAMVDFVKGDLAAAQAKLEAVIAETPDDQGALYNLAIV
                     YFSADQRDKAKETWAKAAAVDATTQLGQMAQQFVDMMDQTAASGSSPHEADTTSTTTK
                     "
     gene            1452..1790
                     /locus_tag="VFD74_00425"
     CDS             1452..1790
                     /locus_tag="VFD74_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZK48057.1"
                     /translation="MFKFLNRAPQGYTDLSPSEVHERLAADGRVQLIDVRSPAEYARG
                     HIPKARLIPLGELESRGEELKKDRDIILYCQSSSRSRHGARILAKLGYENVFNMSGGI
                     VRWPYGVKRS"
     gene            complement(1917..2513)
                     /locus_tag="VFD74_00430"
     CDS             complement(1917..2513)
                     /locus_tag="VFD74_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZK48058.1"
                     /translation="MAKLERIKKHDLLERIAHWVHLGNMILLVLSGMQIHFPAFNIFG
                     SMGNARFVHFVDMYLFFFLGVFHVYQFLTEGKWMTAGPAPRNLRGLGATVKYYLFVSE
                     SKPESGKYNSLQIVTYFLLFAVSALMAIVGFTLYWPLRLAAIVDLFGGLMTLRQIHYV
                     LSWVFISFSIVHIYLILTQPLKYARAMVTGSYWRRVSG"
     gene            complement(2592..4163)
                     /locus_tag="VFD74_00435"
     CDS             complement(2592..4163)
                     /locus_tag="VFD74_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HZK48059.1"
                     /translation="MAKVVIDPVTRIEGHLRVEVEVENGKVVDAWATSTMFRGLEMIV
                     ENRPWMDAWHYTHRVCGVCPTPHGHNSAMALENAAGISPPPNGRLMRNLLECAQLLHD
                     HVLWFYILNGLDYVNVVSALSAKASSSGLKAVQDRLKAFVDSGQLGFLENGYWDHPAY
                     ALDPELNLQLAAHYLESIKVQAVASEASALFGGKFPYQMSTPPGGFASVPHQDQIDQF
                     EAKVREVKGFIDGTLVPDLMAIAPFYLDQAGIGAGHGNYFTWGVIDEDLEGGDPYKRL
                     FPRGGVFGAFDGNLSVQPVDQQTDVREWVTSSWNTQYEGGLHPWEGVTGPDFTGIEGI
                     PEIDRNGRYAWEKSAHMKGAPVEVGPLAQMLVAYLGGPADHPAKKLIDDTLAAIGHAG
                     NPAVLFSNLGRIAARVLKVKIIGDKTLDYIAQLRANMAAGDMNFYTPVPDSPTGQGFS
                     GWDAPRGSLAHWATLEDGRVKHYQIVTPSGWNFAPRAGDGRVRGPVEQALVGTPVADP
                     ERPLEVLRTLHSFDP"
     gene            complement(4164..5270)
                     /locus_tag="VFD74_00440"
     CDS             complement(4164..5270)
                     /locus_tag="VFD74_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HZK48060.1"
                     /translation="MKDIFAARGLTRRDFLKVCGGLAAAMGLGQAAVPGIARALEQAA
                     KRPPVVWLDFQECLGCTESVSKSRYPDFLNIVLDMISLEYSEALMAGAGHQAEAKLKQ
                     TMTDNKGAYVLVVEGSVATKIPSAMTIAGRTSVDIAREALTDAALVIAVGNCASFGNV
                     QAAHPNPSGAMGMLAFMEQEGIDTSKLIQLPTCPVNPIHLTSTITYFLTYGKVPETDQ
                     YRRPKLFFGRKVHDECPRRAHFDEGRFVEALGSPEENLRYCLYKMGCRGPHTYSDCAT
                     VLWNNRSNWCIGTGQCIGCSEKGFWDEFTDFYSPMPGVSIPGFGGVRVGADTVGIGLA
                     AATGVAVAAHLAVSAAKGRLGKNDDGSVAEEKEA"
BASE COUNT          994 a         1735 c         1746 g          968 t
ORIGIN      
        1 gtaccagatc aagccggatc cactgaagag cgccgtgcgt gcctggtcgg gatgggctat
       61 cacgtagtgg agcttggcgc cgaccacgcc cccgactatc gcggcgatga gcatggagta
      121 ggcgagctcg gggtccactc ccctgcgctt catcccccag gccgcgacga ggccggcact
      181 cacgaacgca agggccatca tcacgccgaa cgagtggata gtcagggggc ctattgtgag
      241 aagctgggga tacatgaggc tccgatggca gaggcacccc ccggagccgc agtcagacag
      301 accgcagctc gcggaggggc gggtctctcg ctacttggtc gtcgtgctcg tggtgtcggc
      361 ctcgtgcgga ctgctgccgg aggctgcggt ctggtccatc atgtccacga actgctgggc
      421 catctggccg agttgcgtgg tggcatcgac cgccgcggcc ttcgcccagg tctctttcgc
      481 cttgtcgcgc tggtcggcgg aaaagtagac gatggccagg ttgtagaggg ctccctggtc
      541 gtcgggcgtt tctgcgatca cggcctccag cttcgcctgc gccgcggcca gatcaccctt
      601 gacgaagtcg accatggcga aaccggactt gaccttcgga ttgtccggct ccagagcggc
      661 ggcttcgtcc aagaggcggg tggcctcgtc gggattgttc gcctgcaggt aggcctcggc
      721 cagcgcgagg atgaggtcga cgttcttcgg atccgccttg taggcttcct ccgccgtctt
      781 caccagttct tccgctgtcg ggccggtccc ggtgtctccg gtcgagggat gcccgtccgg
      841 gacggcagca tcgggagata tcggaggcgc cgccaccgtg gtatcggtcg tgccgtccgc
      901 agatcccacc aggttgggga tcgctacgat caggcccaca accactatcg caacaacgat
      961 aatggcgatc aggtactttc tgttcatccg cgcctttcct ttcgagcaac gagtatcctg
     1021 tgtgacaagc ccgcgaggac caaggcgtga tgctacacga tgtagcagac aaaacccacg
     1081 ccccggcacc ggcgggcgct gcgagcatgt gaatcgaacc acatatcgcg gcggatgacc
     1141 actcgatccg gggcaagtga ccggcgactc gcggcaacgc gccttgaatc cggtcgctga
     1201 gtgctctact attcccgcaa tataccccgc agggtatcag gcgaggtcac acgtgaagac
     1261 agcccagata gatttcgatt cgtgccaggc gtgctcacga tgtgacgctc gcaaggcctg
     1321 ccggaccaag gcgatcgtga agatgggccc cgacgagcag gcgatagtca agccatcgga
     1381 ctgcatggga tgcggtgact gtatcgaagc ctgcccgttc ggagcgatct tgatgaagga
     1441 gatgtaacac gatgttcaag ttcttgaatc gggcgccgca aggctacacc gacctatccc
     1501 cctccgaggt ccatgagcgg cttgcggcag acggccgcgt gcagctgatc gacgtccgct
     1561 ccccggccga gtacgcccgc ggacacattc ccaaggcacg gctgatcccg ttgggcgaac
     1621 tcgagagccg cggtgaagag ctgaagaagg atcgagacat catcctctat tgtcagagct
     1681 cttcacgcag ccggcacggc gcgcgtatct tggcgaagct gggatacgag aacgtcttca
     1741 acatgtccgg agggatcgtc cgctggccct acggagtgaa gcgatcgtga cggtcacagc
     1801 ctcctccgat ccggcaggcc tgccctccta cgcgaacgga acaggcgcca tcccgaagga
     1861 cggcgcccgc cgagatgcag gcgctcacgc ggctaggttc ggaggcgcct ccgaacctag
     1921 ccgctcaccc ggcgccagta gctgcccgtg accatggcgc gagcgtactt cagcggctgc
     1981 gtcaagatca ggtagatgtg cacgatgctg aacgagatga acacccacga gagcacgtag
     2041 tggatctgac gaagcgtcat taacccgccg aacaggtcca cgatcgccgc cagcctcagc
     2101 ggccagtaga gcgtgaagcc gacgatcgcc atcagcgccg atacggcgaa cagcaagaag
     2161 tacgtcacga tctggagtga attgtacttg cccgactccg gcttgctctc agagacgaag
     2221 aggtagtact tcaccgtggc ccccagcccc cgcaggttgc gcggcgccgg cccagccgtc
     2281 atccacttgc cctcggtcaa aaactggtac acgtggaaca cgcccaggaa gaagaacagg
     2341 tacatgtcca cgaaatgcac gaaccgagcg ttgcccatgc tgccgaagat gttgaacgcg
     2401 gggaagtgta tctgcatgcc cgacagcacc agcaggatca tgttgcccag atgcacccag
     2461 tgggcgatgc gctccaggag gtcatgcttc ttgatgcgtt ctagcttcgc catgatggca
     2521 tccgcctact tgaccctgaa cttgaggatc ttgttgctct tcggttcaat cacgtgaacc
     2581 gagcaggcca ttcaggggtc gaaggagtgt aacgtccgca gaacctccaa cggcctctcg
     2641 ggatcggcca ccggcgtgcc cacgagtgcc tgctccaccg gcccgcgaac gcgaccgtcg
     2701 cctgcccgcg gggcgaagtt ccatcccgag ggagtgacga tctggtaatg cttcacccga
     2761 ccgtcctcga gtgtagccca gtgcgcgaga gaacctcgag gagcgtccca gccgctgaag
     2821 ccttgacccg tggggctgtc gggcacggga gtatagaagt tcatgtcgcc ggcggccatg
     2881 ttcgcccgca gctgcgcgat gtagtcgagg gtcttgtcgc cgatgatctt gaccttgagc
     2941 acgcgcgccg cgatgcgacc gaggttcgag aagagcacgg ccgggttgcc ggcgtggccg
     3001 atggcggcca aggtgtcgtc gatgagcttc ttggccggat ggtccgccgg gccgcccagg
     3061 taggcgacga gcatctgggc aagcgggccc acctcgaccg gggcgccctt catgtgtgcg
     3121 ctcttctccc aggcgtagcg accgttgcgg tcgatctccg gtatgccctc gatgcccgtg
     3181 aagtccgggc cggtgacgcc ctcccacggg tgcagtccgc cttcgtactg cgtgttccac
     3241 gagctggtta cccactctcg gacgtccgtc tgctggtcca ccggctggac gctcaggttg
     3301 ccgtcgaagg ccccgaagac gccgccccga gggaacaggc gcttgtacgg gtctccccct
     3361 tcgagatcct cgtcgatgac accccaagtg aagtagttgc cgtgcccggc accgatgccg
     3421 gcttgatcca agtagaacgg agcgatggcc atcagatcgg gcaccagggt gccgtcgatg
     3481 aagcccttga cctcacgcac cttggcctcg aactggtcta tctggtcttg gtggggcacc
     3541 gacgcaaacc cgccgggagg ggtggacatc tggtagggga acttgccgcc gaagagcgcg
     3601 gacgcttctg aagccacggc ctgcactttg atggattcga gatagtgagc cgcaagttgc
     3661 aggttgagct cggggtcgag ggcataggcc ggatggtccc agtagccgtt ctcgaggaag
     3721 ccgagctggc ccgaatcgac gaaggccttc aagcgatcct gcacggcctt gaggcccgac
     3781 gacgacgcct tcgccgagag cgcgctcacg acgttcacgt agtcgaggcc gttgagaatg
     3841 tagaaccaga gcacgtgatc gtgcaggagt tgggcgcatt cgagcaggtt gcgcatgaga
     3901 cgtccgttcg gcggcggaga gatgcccgcg gcgttctcaa gcgccatggc cgagttgtgg
     3961 ccgtgcgggg tggggcacac gccgcacacc cggtgcgtgt agtgccaggc gtccatccac
     4021 gggcgattct ccacgatcat ctcgaggcct cggaacatcg tgctcgtggc ccacgcatcg
     4081 accaccttgc cgttctccac ctcgacctcg acgcgcaggt gtccttctat gcgtgtgacc
     4141 ggatcgatga cgaccttggc catctacgcc tccttctctt cagcgacgct accgtcgtcg
     4201 ttcttgccga ggcggccttt cgccgcgctg acggccaagt gcgctgcgac agccacaccg
     4261 gtcgccgcgg cgagcccgat gccgaccgtg tcggctccga cccgcacgcc accgaacccg
     4321 gggatcgata cgcccggcat gggcgagtag aagtcggtga actcgtccca gaagcccttc
     4381 tcggaacacc cgatgcactg tcccgtcccg atgcaccagt tggagcggtt gttccacagc
     4441 acggtggcac agtcggaata ggtatgcgga ccgcggcacc ccatcttgta gaggcagtag
     4501 cgcaggttct cctcagggct gccgagggct tcgacaaaac gaccctcatc gaagtgggcg
     4561 cgcctggggc actcgtcgtg gaccttgcgc ccgaagaaca acttcggccg ccggtactga
     4621 tcagtctcgg ggaccttgcc gtaggtgagg aagtaggtga tggtgctggt gagatggatc
     4681 gggttgaccg ggcaggtggg caactggatg agcttgctgg tatcgatgcc ctcctgctcc
     4741 atgaacgcca gcatccccat agccccgctg ggattcggat gggcggcctg gacgtttccg
     4801 aacgaggcgc agttgcccac cgcgatgacc aaggctgcgt cggtgagagc ctctcgggct
     4861 atgtcgaccg aggtgcggcc tgcgatcgtc atggcgctcg ggatcttcgt cgccacagag
     4921 ccttcgacca ccagcacgta ggcgcctttg ttgtcggtca tggtctgctt gagcttggct
     4981 tccgcttgat gcccggctcc ggccatgagg gcctcggagt actcgagact gatcatgtcg
     5041 agcacgatgt tgagaaaatc ggggtagcga ctcttggaca ccgactcggt gcagccgaga
     5101 cactcctgga aatcgagcca gaccaccggc ggacgcttcg ccgcctgctc cagcgctcgg
     5161 gcgatccccg gaacggctgc ctgaccgagc cccatggcgg ccgcgaggcc gccgcagacc
     5221 ttcaggaaat ctcggcgggt gagcccgcgc gcggcgaaga tatccttcac gacctcgccc
     5281 ccttcaccac cgacctaaga gcgacgacca cagtcgaggc cgcggcggcg gcgtagagga
     5341 acttgaagaa ctcgatgcga gtgaccctca aggcggacct cccctccatt ctcccttggc
     5401 tccacggcgg ggcaccgacg gcggcgcggc ggcctagccg gag
//
LOCUS       DASRMJ010000019         4726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_435692, whole genome shotgun sequence.
ACCESSION   DASRMJ010000019 DASRMJ010000000
VERSION     DASRMJ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435692"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFD74_00445"
     CDS             <1..586
                     /locus_tag="VFD74_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZK48061.1"
                     /translation="VTIVEELGEVVRHFVADDYESLAVTVAALDRLESAADDRKADIL
                     DKLAGGGVFTMGRADLSRLVASVDGIANYAAGAADRIVMRRFTLPPELNKLLVEMVKI
                     DLEAVRKLRDAVFALRADMREAIEIAIEVDAIESRADKVFAQMYHFMFDMETDFKTFH
                     QLKAIIERLESIADKCAENSELIRHMALGYLDLG"
     gene            complement(583..2427)
                     /locus_tag="VFD74_00450"
     CDS             complement(583..2427)
                     /locus_tag="VFD74_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZK48062.1"
                     /translation="MHLNVRRRLADRIWQSRKITYAVSSFVVALGFIAVFLFISAPTA
                     EIPVSTKPTLFEAGRAFRVAQELADLYPEPAVGSEESSLAAAWVADRLTTLEIPHQEA
                     DFTVPLGDREVVMRNVAVVFTGSSREAILISASRDPDLDSRLSPLANATGTAILIDLA
                     QAFAARPHERTIILLSTEGGSYGGLGLNHFLETDPLAADVRAILSLRGLGREKRDVLR
                     AAVSGTDTATPGWYVQLVSQTLGKTGIGLEFNGLARQVADEALQLARGEQVAGLRAGV
                     PSIMLYDPQPGEPTSAGIATQGTSVERIILSLDSGSEIPRDPGTAVVLGSGRYVTTRA
                     LSLLGVLMLLPAVAMALTWLAVSRVRPEVWLRYLRNLVSFALPLAVWCAMVLAAARVG
                     ILPRYLFQAPVTARVATEPDFLVVALLAVLGIAVLFLSRHYLGHLQPREPLIMAGTVK
                     LSTGLLAFVLGLAVLTAHSPLSLLTGVTAAWMWPLVTCFAEPRSASLSWLPRARGNAV
                     LLLAGLAGPIVLYLYLVLSTNIRWWQGWWFLMIQAVSGAYGILGPVASVLITTGFFTL
                     LGVKRLQLIPLETLDDRDDFDLVEAPPRVRRVKKPHHPDKDRLPPPGP"
     gene            2628..3848
                     /gene="glp"
                     /locus_tag="VFD74_00455"
     CDS             2628..3848
                     /gene="glp"
                     /locus_tag="VFD74_00455"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZK48063.1"
                     /translation="MVTIEEARALVLAEASPLPSELRSLSEVLGGVLTRELVAPHDIP
                     PFDNSAMDGYAVRAVDTVGAPDAPVTLAVVDTVAAGRVARAEVGPGESIKIMTGAPLP
                     LGADAVVEVEATEPGDGTVVVLRELAPGENVRRAGEDVRRGTPVLAAGDIVGPAEVGL
                     AAGLGFPFLEVHRRPRVAIVATGSELVEVSEQLAPGLIRNSNSYTMEAQCRQLGIDAE
                     RLGIAPDEYQATRSLLARGMTYDVLITSGGVSVGEFDFVKEVQDDLGVERRLWRVAMK
                     PGKPLVFGVKDGCLVFGVPGNPVAAMISFELFIRPALLRLMGHRKVLRPVYQAVMHED
                     LVNRYGRVHVVRVRAWHEGGMWHASSTGPQGSGILKSMVNADGLVFVPADTGGIRAGE
                     VVDMTLLREQAREA"
     gene            4108..4293
                     /locus_tag="VFD74_00460"
     CDS             4108..4293
                     /locus_tag="VFD74_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48064.1"
                     /translation="MANHFTPEELAREFGMDIRDVIQLCIEESVPIYKGKVDRSLLSL
                     VMKSRGMTVESREAAAV"
     gene            complement(4249..>4726)
                     /locus_tag="VFD74_00465"
     CDS             complement(4249..>4726)
                     /locus_tag="VFD74_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48065.1"
                     /translation="FEEAQAVREALDDLDTLRRSYRALAEARTLRAAVMWPGGADHGR
                     RTVHLDLVWSGSLRASGRLDRTNAETEIERMLGALPEPENPDTRIAVPQEQLDLLLAV
                     RRWIGGAPASSLTPFPAGLELGAATEGWRQDILAAALRLLDSSDCGGLSALDGHSP"
BASE COUNT          810 a         1509 c         1646 g          761 t
ORIGIN      
        1 ggtcaccatc gtcgaggaac tcggggaagt ggtgcggcac ttcgtcgccg acgactacga
       61 gtcgctcgcc gtcaccgtcg ccgcactcga tcggttggag agtgcagcgg acgatcgcaa
      121 ggccgacatc ctcgacaagc ttgcgggggg cggggtcttc acgatgggcc gggccgatct
      181 ctcgcgtctc gtggcgtccg tggacgggat agccaactac gcggcaggag ccgccgaccg
      241 tattgtcatg cgtcgcttca ccctgcctcc cgaactcaac aagctgctgg tcgagatggt
      301 gaagatcgac ttggaggccg tgcgcaagct gcgcgatgcc gtgtttgcgc tgcgggccga
      361 catgcgtgag gccatcgaga tcgcgatcga agtggacgcc atcgaaagtc gcgcggacaa
      421 agtcttcgcg cagatgtacc acttcatgtt cgacatggag acagacttca agaccttcca
      481 ccagctgaag gccataatcg agcgcctcga gtccatcgcc gacaagtgcg cggagaactc
      541 cgaactgatc cggcacatgg cgttggggta tctcgacctg ggctagggtc cgggcggcgg
      601 gagccggtcc ttgtcggggt ggtgcggctt cttcacccga cggacccgtg gtggcgcctc
      661 caccaagtcg aagtcgtcgc ggtcgtccag cgtctctagg gggatgagct gcaggcgctt
      721 caccccgagg agggtgaaga agcccgtggt gatcagcacc gacgccaccg gccccaggat
      781 cccgtaggca cctgagaccg cctggatcat caggaaccac cagccctgcc accaccgtat
      841 gttggtgctc aacaccaggt agaggtagag cacgatgggg ccggccagac ccgcgagcaa
      901 gagcacggcg ttgccccgcg cccgcggcag ccacgagaga gacgcgctgc ggggctcggc
      961 gaagcaggtg accaggggcc acatccaagc cgccgtgact ccggtcagaa gcgacaaggg
     1021 cgagtgagcg gtgagcactg ccagcccgag tacgaacgcc agcaacccgg tgctcaactt
     1081 gacggtcccg gccatgatca gaggctcccg gggctgcagg tgcccgaggt agtgtctgct
     1141 gaggaacaag acggcgatcc ccagcaccgc gagcaacgcg accacgagga agtccggctc
     1201 ggtggcgacg cgcgcggtga cgggggcctg gaacagatac cgcggcagga tcccgacacg
     1261 cgccgccgcg agcaccatcg cgcaccagac cgccaagggc agggcgaacg acaccaggtt
     1321 gcgaaggtag cgcagccaca cctccggccg cactcgggat accgccagcc acgtgagggc
     1381 catggccacg gcggggagga gcatgagtac cccgagcaga gagagtgccc gggtggtgac
     1441 gtagcgaccg gacccgagca cgaccgcggt ccccgggtcg cgcgggatct cggacccgct
     1501 gtccaggctc aagatgatcc gctccaccga ggtgccctgg gtggcgatgc ccgcggacgt
     1561 gggttctccc ggttgggggt cgtagagcat gatcgacggc acccccgcac gcaggccggc
     1621 cacctgttct ccccgggcca gttgcagtgc ctcatcggcg acctgccgag ccaggccgtt
     1681 gaactctagg ccgatccccg tcttgcccag cgtctgcgac actagttgga cataccagcc
     1741 cggtgtggcg gtatcggtgc ccgacacggc agcacgcaag acatcgcgct tctctctccc
     1801 gaggccacgg aggctgagga tggcgcgcac gtcggcggcg agtgggtcgg tctcgaggaa
     1861 gtggttcagt ccgagcccgc cgtagctccc gccctcggtg gagaggagga tgatggtgcg
     1921 ctcgtgcggt cgcgcggcga aggcttgagc caggtcgatc aggatggccg tcccagtcgc
     1981 gttcgcgagg ggcgagaggc gcgagtcgag gtcggggtcc ctcgaagccg agatcaggat
     2041 ggcttcccgg ctcgagccgg tgaagaccac ggccacgttc cgcatcacca cctcccgatc
     2101 ccctagcggc acggtgaaat cggcctcttg atgcggtatc tccagagtgg tcaaacgatc
     2161 cgcgacccag gccgccgcca gtgacgactc ctcgctgccc acggccggct ctgggtaaag
     2221 gtcggccaac tcttgggcga ctcgaaaggc tcttccggct tcaaagagcg tgggcttcgt
     2281 ggagaccggg atctccgcgg tgggagcact gatgaagagg aagaccgcta tgaagcccag
     2341 cgcgaccacg aacgaactca ccgcgtaggt gatcttcctc gactgccaga tacggtccgc
     2401 caggcggcgg cgcacgttga gatgcatcgt ctctccagcg tcgggggctt ggccgtctgc
     2461 tgcgcgtact ccggagtgca ttgtagggcg tgtggcggga tgctacaaac tcccggcctg
     2521 cgggaggcgc gggcgcaggc tctcgcagaa tgaggtcatg ctatcctgat gcatcgatcg
     2581 agggtcacca ctcccgcgta cgcagggtgc aatcggaggt cactgccatg gtcactatcg
     2641 aggaggcgcg ggcgctcgtc ctcgctgaag catcgcctct gccctccgag ctgaggtcgc
     2701 tctccgaggt cttgggtggt gtgctcactc gtgagttggt cgccccgcac gatatccctc
     2761 cgttcgacaa ctccgccatg gacggctacg cggtgcgggc cgtcgacacc gtcggcgctc
     2821 ccgatgcgcc ggtgaccctc gccgtcgtgg acacggtggc ggccggccga gtggctcggg
     2881 ctgaggtcgg ccctggtgag tccatcaaga tcatgaccgg agcgcccttg ccgctcggcg
     2941 cggatgccgt cgtggaggtc gaggccaccg agccgggtga cggcaccgtg gtcgtgctgc
     3001 gcgagctggc gcccggcgag aacgtgagac gcgccggtga agacgtccgg cgcggcactc
     3061 ccgtcctggc cgccggcgac atcgtcggtc cggccgaggt cggcctcgcc gccggcctcg
     3121 gattcccgtt cctcgaggtg caccgccgcc cgcgagtggc tatcgtggcc actgggagcg
     3181 aactcgtcga ggtgagcgag cagctggcac cggggttgat ccgcaactcc aacagctaca
     3241 ccatggaggc ccagtgccgc caactgggca tcgacgcgga acgcctcggc atcgcacccg
     3301 acgagtacca ggcgacgcgc tcccttctcg cgcgcggcat gacctacgac gtgctgatca
     3361 cttccggcgg ggtgtcggtg ggagagttcg acttcgtcaa ggaggtgcag gacgatctcg
     3421 gcgtggaacg acgtctgtgg cgcgtggcga tgaagccggg caagccgctg gtcttcggcg
     3481 tcaaagacgg gtgcctcgtg ttcggcgtgc cgggcaatcc ggtggccgcg atgatctcct
     3541 tcgaactctt catacggcct gcgttgctcc gtctcatggg ccatcgcaag gttctgcgtc
     3601 cggtgtacca ggccgtgatg cacgaggacc tcgtgaaccg ctacgggagg gtgcacgtgg
     3661 tgcgggtgcg cgcctggcac gagggcggga tgtggcacgc cagctccacc ggtcctcagg
     3721 gctccgggat actcaagtcc atggtgaacg ccgacggcct ggtgttcgtt ccggccgata
     3781 ccggaggtat ccgggcgggc gaggtggtcg acatgacgct cctgcgggag caggcgcgtg
     3841 aggcgtgacc tcgggaggtc ccgtgtcgcg aaaagcgtcg tctccggctt gtgcgccgac
     3901 gcttcggcag ctacaatgaa cccgtcatga ttcctcgaat ggatcagggg gagttccccc
     3961 gccggggaga agggaagcgt cccgttctcc ggtcaacacc atcacaatag cctggcaggc
     4021 tgctcgctgc cgggcttttt atttgtgtcc aagaacggcg gttctcaacg gggctgtggc
     4081 cccagtccgc cctggaaagg agaagtgatg gcgaaccact tcacccccga agagctggcg
     4141 cgagagttcg gaatggatat cagggatgtg atccagctct gtatcgagga gagtgtgccc
     4201 atctataaag gtaaggtcga tcgtagtctc ctgtctctgg tcatgaagtc acggggaatg
     4261 accgtcgaga gcagagaggc cgccgcagtc tgacgagtcg agcaggcgga gggcggcggc
     4321 gaggatgtct tgtcgccacc cctccgtcgc cgcaccgagt tcaagccccg cgggaaacgg
     4381 ggtgagcgaa gaggccggtg cgcccccgat ccatcggcgc accgccagga gcagatcgag
     4441 ctgttcctgc ggcacggcga tgcgagtgtc gggattctcg ggttcgggga gagcacccag
     4501 catcctctcg atctcggttt cggcgttggt gcggtcgagt cgccccgacg cccggagtga
     4561 accgctccac accagatcca gatgcacggt gcgccggccg tggtcggcgc cgcccggcca
     4621 catgactgcc gcgcgcaagg tccgggcctc ggcgagagcg cggtaggagc gccgaagggt
     4681 atcgaggtcg tcgagggctt cccgcaccgc ctgcgcctcc tcgaag
//
LOCUS       DASRMJ010000020         3427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_254111, whole genome shotgun sequence.
ACCESSION   DASRMJ010000020 DASRMJ010000000
VERSION     DASRMJ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254111"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFD74_00470"
     CDS             <1..194
                     /locus_tag="VFD74_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48066.1"
                     /translation="STSVRSAIRHLGRPPMSRDPRDFVTNLHAPMPFSRKVYLVLRNN
                     WIKIRTGSTCCGHPGEPGC"
     gene            complement(412..1398)
                     /locus_tag="VFD74_00475"
     CDS             complement(412..1398)
                     /locus_tag="VFD74_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZK48067.1"
                     /translation="MRVSISGATGFMGRSITTHLLLQGHAVRAVSRSAKKAQGTLASL
                     PGASAALGEGRLTFVTANVTDPATLGPAIADSDVVIQCAQFPGAPVEDARHGLTYMKV
                     DRDGTLNLLAAIAAHYGARTAGPGLTRFPDGAPRFFYLSGVTVSASAPEAWNRAKWQA
                     EEATRGSGLDWTIVRSSWAFGPGDVALNRIIGYSGVLPFVPVFGSGEERLTPVFVDDV
                     GRLFALLTEDPLASRDATIPLGGPDVFTMNEFLRVVLDVMGRRRPILHVPKPVAKIPA
                     AVLQYLPGRPLSPAAVDFASQGGVADPTALREGFPDFAPTPTRAALQSYLGS"
     gene            complement(1480..2181)
                     /locus_tag="VFD74_00480"
                     /pseudo
     CDS             complement(1480..2181)
                     /locus_tag="VFD74_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018245191.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
     gene            2274..2516
                     /locus_tag="VFD74_00485"
     CDS             2274..2516
                     /locus_tag="VFD74_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HZK48068.1"
                     /translation="MQQRRCPRHGPHDTLIRSSRFHREHLAFRDALRADARLREEHQP
                     LKSRLAAEFGSDREGYGRAKSGFIEAALSRKRRRRR"
     gene            2522..2986
                     /gene="mscL"
                     /locus_tag="VFD74_00490"
     CDS             2522..2986
                     /gene="mscL"
                     /locus_tag="VFD74_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZK48069.1"
                     /translation="MFKEFKEFAMRGNVLDMAVGIIIGAAFGAIVTSLVADVIMPPIG
                     LLLGSVDFSNLFVIIKQGSTPAPYDSLTAAKDAGAVTINYGIFILSVISFLIVASVVF
                     MLVRSVNRLKREDAVAAEEAEPDARECPFCFTSISAKARRCPNCTSQLDGAA"
     gene            3095..>3427
                     /locus_tag="VFD74_00495"
     CDS             3095..>3427
                     /locus_tag="VFD74_00495"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZK48070.1"
                     /translation="MDFLTVHRETLINLGYALLILAGAWVAVRLMRRAVDRFLTHRAL
                     AELDAGAQTRFRMIERLAAAALIFLGLGLALYMVDVGVLRKISVAMFASAGLVGIALG
                     FAAQTTVAN"
BASE COUNT          523 a         1154 c         1141 g          609 t
ORIGIN      
        1 tttctacttc tgtccgctct gcgatccgcc atctggggag gccgccgatg tcgcgtgacc
       61 cgcgggactt cgtcaccaac cttcacgcgc caatgccgtt ctcgcggaag gtctacctcg
      121 ttctgcgcaa caactggatc aagatccgaa cgggaagcac gtgctgcggg cacccgggtg
      181 agccggggtg ctgagaggga gcaccggcgc cacgtgatgg cgcatcgcct agcagcactc
      241 agacggccga tgaccacaac catcatactc acgaacatgc acacgatctg ccgccagatg
      301 gggactgaga gcgtcgcttc cggctgcgga ctgcctatcg gcgacttctg cgcgacaccc
      361 cgatcgccac gagcgggagt tgcgaccctc agcgctccgg gccgggcacg atcatgagcc
      421 caggtagctc tggagcgcag cgcgggttgg agtgggggcg aagtcgggga acccttctct
      481 gagggcggtc gggtcggcga ccccgccctg agaggcgaag tccaccgccg cgggcgagag
      541 tggacgcccc ggcaggtatt ggagcacggc cgcggggatc ttggccacag gcttgggcac
      601 gtgcagtatc gggcggcgcc tgcccatgac gtcgagaacc acccgcagga actcgttcat
      661 ggtgaacacg tcggggccgc ccaaggggat ggtggcgtcc cgggacgcca acgggtcctc
      721 ggtcaggagc gcgaagaggc gaccgacgtc gtccacgaac acgggggtga gtcgctcctc
      781 accgctgccg aacaccggca cgaacggcag cacgccggag tatccgatga tccggttcag
      841 tgctacgtct ccagggccga acgcccagct cgagcgaacg atagtccagt ccaagccgct
      901 tccgcgggtg gcctcctccg cctgccactt ggccctgttc cacgcctcgg gagcggaggc
      961 cgacaccgtc accccgctca agtagaagaa gcggggtgct ccgtcgggga agcgggtcag
     1021 tcccgggccg gcggtgcgcg cgccgtagtg cgcggcgatg gcggccagca gattgagggt
     1081 gccgtcacgg tcgaccttca tgtaggtgag gccgtgccga gcgtcttcca ccggggctcc
     1141 ggggaattgc gcgcactgga tcacgacgtc cgaatccgcg atggcggggc cgagagtggc
     1201 tggatccgtg acgttggcgg tgacgaaggt gaggcgaccc tcgcccagcg cggcagaagc
     1261 gccgggcagg ctcgccagcg tgccctgcgc cttcttggcc gatcggctca cagcacgcac
     1321 cgcgtgtccc tgcagcagca ggtgagtggt gatggaccta cccatgaacc cggttgctcc
     1381 ggagatcgac actctcatgc tgacactccc tccatcacat gccccccgat tagatgtctt
     1441 gcggcctgca gcgccgctgt gcttctttca tgtggccggg cggccagcag ccacctcttc
     1501 gatgttgcgg ccggacgcga ccgagagcgg cgcttcgtcg acgatcgcgg agccgggtcg
     1561 cgacgcctcg tcccggtgct tgatgagcag ccagttcctc tctccacgcc ccttcatctg
     1621 cacgagggtc cagcttccgg tgagcttatg cccgtgcagg atgaacttga gttcgcccgt
     1681 ggccaggtcg agggcggcgt cggtgtcagc cgcgcctgca gcgcggtcat gggcggtttc
     1741 ggacgcgtcg cgcggcgctc cggtcgggcc gccctccttc gtgcgcgggc ggtcgggctc
     1801 ccaccacccg aagtcccaga ggacgaccgt tccaccgccg tactctcctt cggggatggt
     1861 tccctcgaag ttgccgtatt cgagcgggtg gtcctcggtc atgaccgcca accgcttgtc
     1921 cgtgggatcg agagacgggc cttttggcac cgcccacgac ttgagcacgc cgtccatctc
     1981 aagacgcgcg tcgtagtgca gggagcttgc cgcatgcttc tgcacacaga agatcggccg
     2041 gtcgcgacgt gtcggatgct cgtcgtctcc ggtcggctcc gcggtgagtc caaagtctcg
     2101 tttctcgcgg tagtccctga gctggtcgtg ggcgctcggc acgtcgcccc ccttctgttg
     2161 ggcgctgcat ccggtcacca taccgcgctc gaccagtccc tacacacgcc ggcccccgcg
     2221 tggtcggcgc acgtgtcgcg tcgctcgcat cggagaggct cgaggcacgg gctgtgcagc
     2281 agcggcgttg cccgcggcat ggtccccacg atacgctcat tcggtcttca cgtttccacc
     2341 gggagcatct ggcgtttcgc gacgccttgc gtgcggatgc gcgtcttcgg gaagagcacc
     2401 aacctctcaa gtcccgcctg gcggctgagt tcggctcgga ccgggaaggc tacggtcggg
     2461 ccaagtccgg gttcatcgag gcggcgttga gtcggaagcg gagaaggagg aggtagctca
     2521 catgttcaag gagttcaagg agttcgccat gcgcggcaac gtgctcgaca tggcggtggg
     2581 catcatcatc ggtgcggcgt tcggcgccat cgtgacctcg ctcgtggccg acgtgatcat
     2641 gccacccatc ggcctcttgc tcgggagcgt ggatttcagc aatctgttcg tgatcatcaa
     2701 gcagggctcg accccggcgc cgtacgactc tctgactgcg gccaaggacg cgggcgccgt
     2761 gaccatcaac tacggcatct tcatcctcag cgtgatcagc ttcctgatcg tggcctctgt
     2821 ggtcttcatg ctggtgcgct cggtcaaccg actcaagcgc gaggacgcgg tggcggcgga
     2881 ggaagccgag cccgacgccc gagagtgccc cttctgcttc acctccatct cggcgaaagc
     2941 gcgccgctgt ccgaactgca catctcagct ggacggtgcc gcgtagttgc agcgcgcgcg
     3001 ggtgccgggt ggtgtcgcgc agcgcgccag gtacaccggg tgcttctcgc cgcacgtccg
     3061 acgaccgctc acgatcctgc gatatagtgg gcgcgtggac ttcctgaccg ttcatcgcga
     3121 gacgctgatc aacctgggct acgccctgct catcctcgcg ggcgcgtggg tcgcggtgcg
     3181 gctcatgcgc cgggcggtcg atcgcttcct cacccaccgc gcactcgccg aactggacgc
     3241 cggtgctcag acccgcttcc gcatgatcga acggttggcc gcagccgcgc ttatcttcct
     3301 cggactcggg cttgcactct acatggtcga cgtaggagtc ctccgcaaga tctcggtggc
     3361 catgttcgcc tccgccggac tcgtcggtat cgccctgggc ttcgccgcgc agacgaccgt
     3421 cgccaac
//
LOCUS       DASRMJ010000021         3117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_507085, whole genome shotgun sequence.
ACCESSION   DASRMJ010000021 DASRMJ010000000
VERSION     DASRMJ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507085"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(444..1463)
                     /locus_tag="VFD74_00500"
     CDS             complement(444..1463)
                     /locus_tag="VFD74_00500"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZK48071.1"
                     /translation="MYESSTFIEGVPKAELHVHIEGTLEPELKFELAARNGLALPYRS
                     VDEVRAAYDFDDLPSFLAVYYEGMSVLLTERDFYDLAMAYFRKAHSQNVVYAEVFFDA
                     QAHTTRGIGFDTFMEGLLRARRDAEASLGLRTQLIMCFLRDMTAASAGVTLERALPYR
                     DVIVGVGLDSDERDNPPVKFKEVFARARADGFHLTVHCDVDQKNSVQHIWQCLNDIGA
                     ERIDHGVNTVEDATLVDEINQRGLGLTVCPISNSYVTDGLKADEIKRLLDLDVRVTVN
                     SDDPAYFPGYMNENLIALQEAASLTRDEIVRVVKNAFSISWLPDDDKDRYLGDVDAYA
                     ASSGS"
     gene            2315..2524
                     /locus_tag="VFD74_00505"
     CDS             2315..2524
                     /locus_tag="VFD74_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48072.1"
                     /translation="MAVEIEYRMKMIEYRLARLRTEAERERLAAMHARRSSARLWLGR
                     ALVTFGRFVEGTGAGEQECGSSIRA"
     gene            complement(2899..>3117)
                     /locus_tag="VFD74_00510"
     CDS             complement(2899..>3117)
                     /locus_tag="VFD74_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK48073.1"
                     /translation="APIFAPLSPREVEILDNIAQGMTNKQVAYALSISEQTVKNHMSS
                     ILRKLSVNDRTQAVVYAMRQGWIRMPAD"
BASE COUNT          522 a         1043 c         1007 g          545 t
ORIGIN      
        1 ggtattcagc caccgcataa tgatgatcct cgaccgtccc agggagcgtg ggctattcgc
       61 gcacgctggc tgaccgctgg cggcccatca ctggctcagc cttcgatcag gcgtcgccgt
      121 aagtgatgcc gcacaatcgg ccggaaccga cccgcagagg agcccacggc tgagattaag
      181 ccgccgccgc gttggatgat ccgactctac gtactcgccg tgctgagccg gagagcaagc
      241 ctcgtcaggg ccgccacgtg gcggaccgcc tcgactgccc gcctgaggtg gacgagctat
      301 accacctgct tgctgtccca cctcggtctg cccctagacc attccagttg agctctcgac
      361 ccgctcgtcg acgtgaggcg atcatcaacc ctgggacacg ggtcgagaca gcgcaagggg
      421 aaggtggccc ccaatgacgc gcgtcagctg cccgacgagg cagcatatgc gtcgacgtcg
      481 ccaaggtacc gatccttgtc gtcgtcgggg agccagctga tagagaaggc gttcttcacg
      541 acgcgaacga tctcgtcccg cgtcaggctg gccgcttcct gcagcgcgat caggttctcg
      601 ttcatgtatc cagggaagta ggcgggatcg tcggagttca ctgtcacccg cacgtccaga
      661 tcaagcaggc gcttgatctc gtccgccttc aggccatcgg taacgtagct gttggagatc
      721 ggacacacgg tcaggccgag ccctcgctgg ttgatctcgt cgaccagcgt cgcgtcctcc
      781 accgtgttga cgccgtggtc gatccgttct gccccaatat cgttcaggca ttgccagatg
      841 tgctggacgg agttcttctg atccacgtcg cagtgcaccg tgaggtgaaa gccatcggcg
      901 cgcgcgcgcg cgaacacctc cttgaacttg accggcgggt tgtctctctc gtccgagtcc
      961 aggcccaccc cgacgatcac gtccctgtac ggcagggccc gttcgagggt cacgcccgct
     1021 gacgcggcgg tcatgtcccg caagaagcac atgatcagct gggtacgcag accaagggag
     1081 gcctctgcgt cccgccgggc tcggaggagg ccctccatga acgtgtcgaa cccgatgccg
     1141 cgggtcgtgt gggcctgggc atcgaagaag acctcggcgt agaccacgtt ctgggagtgc
     1201 gccttccgga agtacgccat ggcgaggtcg tagaagtctc gctcggtcag cagcacgctc
     1261 atgccttcgt agtacacggc gaggaacgac ggcagatcgt cgaagtcgta ggcggcgcgg
     1321 acctcgtcga ccgagcggta aggtagtgcg aggccgttgc gtgcagcgag ctcaaacttc
     1381 agctcgggct ccagcgtccc ttcgatgtgc acgtgcagct ctgccttggg caccccctcg
     1441 atgaatgtcg agctttcgta catcctgctt ctcccgaact ctgctgctag tccgaacctg
     1501 cgggccgcag gccgattcca agaccggctc gaaccgcctc atgttggcta cgtcgatggc
     1561 gccgtcggcg cgatcacgtc cgccgcctcg tgcgtcacgc gagcctgcgg cccttcatgg
     1621 cccggtccct cgtcgttctt tcacgatggc caccaacgcc tcagctcatc ggcagctcgg
     1681 cggtaacgga tatgactgtc tccaccaggt cggttgtatg ggcccggatg tggatccgct
     1741 caccaccgcg agcggtgccg tgaggcggac cgcggcgacc gagagatggt cgaaccagtg
     1801 gatgacggtg ccaatcgcct cggcgggcgg gtgcacctcg gacatgctgg gcctccttcc
     1861 tgccccacgc cgtctcgggt ccacgctaca cccgagcgac acgctggtcg aggaggaggc
     1921 tccatcggtc ggcacgcgca gggttgacgg aaggtccctc cccgccctcg cgcgtagccc
     1981 acgttcacga tgccgtgacg ccctgtcgcg ggctggcgcc cacgccgtgt gctcgcccgg
     2041 cgtactggcc ggctggctgc cgggcctacc cagcggtgac ccatacacga tgcgcggatc
     2101 gtctccctcg tttctatacc atcgaggatg acaaagccac tggccgcttc tcgggtgaca
     2161 ctttcactag ccggcgacac gatgcgtctt catcacttga cgcgccaccg gacatgttag
     2221 ataattatct cacacacggg agatgcacag gactgcaatg gtcgccacga acaacccctg
     2281 gggcacacac cacacgatag gaggggacgc accgatggct gtcgagatcg agtaccggat
     2341 gaagatgatc gaataccggc tggcgcggct ccgcacggaa gcggagcgcg agcggttggc
     2401 cgcgatgcac gcgcgccgct cgtcggcgcg gctgtggctc gggcgcgcgc tcgtgacgtt
     2461 cgggcgcttc gtcgagggca ccggcgcggg cgagcaggag tgcggaagct cgattcgggc
     2521 ctgaacggcg cgacgggttg ccggcgcaac acgatgtccg cattgtgtcg agtcgggctc
     2581 cgcctcgacc catggattgg cggagggacc gcgctcggca acggcaccat tcgcgcagcg
     2641 ggcgatccca gcctggaggt gacccatggc cgcccgcgac cccgctgaag ccctcgccct
     2701 ctggagatac cacctgatcg cggaggcgct cgaccccaag ctcggagcgg cagcgtgcgc
     2761 cgggcctcga gccaaaggtc acgccagcgg gccgtcacgg ccgccacgcc gccggtcccg
     2821 ccgtgttcag gacgccccca ggttcaccgc tttcacctcg gcggctccca aagcacatgc
     2881 gattgtggcc gggccagcct aatcggccgg catccggatc cagccctggc gcatggcata
     2941 gaccacggcc tgggtccggt cgttgacgga gagcttccgg aggatggagc tcatgtggtt
     3001 cttgacggtc tgctcgctga tcgagagcgc gtaggcgacc tgcttgttgg tcatgccctg
     3061 ggcaatgttg tccaggatct ccacctcgcg aggggagagc ggcgcgaaga tcggggc
//
LOCUS       DASRMJ010000022         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_145798, whole genome shotgun sequence.
ACCESSION   DASRMJ010000022 DASRMJ010000000
VERSION     DASRMJ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145798"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VFD74_00515"
     CDS             complement(<1..1160)
                     /locus_tag="VFD74_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZK48074.1"
                     /translation="MRDEYTHTASPVDLGIAARSVNRNLPSAELTARALARGEGILAA
                     NGALVVRTGACTGRSPHDRFIVDESPATETVDWNRVNRPCSPGLFDTMLRKTNRYLEG
                     RDLYVFDGYVGAEWTYQLPIRVIADAAWHALFAQTLFIPPVPADLPHFAPELTVMACG
                     GLRADPAVDGTDGEAFIGISLGRKTVLIVGSSYAGEIKKAVFSVMNYLLPDRGVLPMH
                     CAANMGPGGDVALFFGLSGTGKTTLSAAPGRRLIGDDEHGWSAQCVFNLEGGCYAKVI
                     DLDPDDEPQIYDAIRFGSILENVVVDPQTRAIAYGSSEITENTRATYPVSHIPGAVVP
                     GVAGHPHHVFFLTCDAFGVLPPIARLSPVEAQYHFLSGFTAKLGGTEVGVTEP"
     gene            complement(1616..1689)
                     /locus_tag="VFD74_00520"
     tRNA            complement(1616..1689)
                     /locus_tag="VFD74_00520"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1653..1655),aa:Ile,seq:cat)
     gene            complement(1978..>2208)
                     /locus_tag="VFD74_00525"
     CDS             complement(1978..>2208)
                     /locus_tag="VFD74_00525"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZK48075.1"
                     /translation="SRFHLTFKGRAPVTKALPYEWYNTPNIRSLSIEDFRVFAREQGC
                     RIVRSVHLGPRHRVRFWPNVLAEHGVFVLTRA"
BASE COUNT          386 a          768 c          726 g          328 t
ORIGIN      
        1 ggctcggtca cccccacttc ggtccccccg agcttggccg tgaagccgga gaggaagtgg
       61 tactgggctt ccacgggcga gagtcgggcg atgggaggaa gaaccccgaa ggcgtcgcag
      121 gtgagaaaga acacgtggtg gggatgaccg gccacgccgg gtacgactgc tcccgggatg
      181 tggcttaccg gatatgtggc gcgcgtattc tccgtgatct cagacgagcc gtaggcgatg
      241 gccctggtct gaggatccac gaccacgttc tcgagtatcg acccgaagcg gatggcgtca
      301 tagatctggg gctcatcgtc ggggtccagg tcgatcacct tggcgtagca gccgccctcg
      361 agattgaaga cgcactgcgc cgaccagccg tgctcgtcgt cgccgatcag ccggcggccg
      421 ggagcggcag agagggtggt cttgccggtg cccgagaggc cgaagaagag ggcgacgtcc
      481 ccccccgggc ccatgttggc cgcacagtgc atgggcagca cgccccggtc gggcagcagg
      541 tagttcatca ctgagaatac ggccttcttg atctccccgg cataggaaga gcccacgatg
      601 agcaccgtct tgcgccccaa gctgatgccg atgaacgctt cgccatccgt gccgtctacc
      661 gcagggtccg cccggagacc tccgcaggcc atgacggtga gctcgggcgc gaagtgcggc
      721 agatcggccg gcacgggcgg gatgaagagg gtctgagcga acagagcgtg ccaggccgcg
      781 tccgcgatca cgcggatggg caactgatag gtccattccg ctcccacgta cccgtcgaac
      841 acgtagaggt cccgtccctc gagatagcgg ttggtcttcc gcaacatcgt gtcgaagagt
      901 ccgggcgagc agggtcggtt gacacggttc cagtcgacgg tttcggtggc gggggattcg
      961 tccacgataa accgatcgtg cggcgaacgc ccggtgcagg cgccggtgcg caccacaagg
     1021 gctccgttcg ctgcgaggat cccctcgccc ctcgccaggg cgcgcgccgt caactccgcc
     1081 gacggcaggt ttcggttcac cgatcgggcc gcgatcccga ggtcaacggg ggatgccgtg
     1141 tgcgtgtatt cgtcgcgcat caagcccacc tcacgttcgt ccgccctagt cccgcagacc
     1201 gcagaccgca gaccgcagac cgcagaccgc aatcccatta gacaacgcgc cgcacaaccg
     1261 cgtcaagcgg gcagggcaaa gcggtcgcgt ggcacagact gccaactccg gtccaaactt
     1321 gcgccacttc ccactcccct tgctcggcct gcatgacttc cctgccctcg ccttcaaggc
     1381 ggcagctatc tccccgcgct gcgaagaagt cgacgccctg cgcaaggcgg tccacgattg
     1441 cgaccacagc tacacgctcc cgggaggcct cggctacgcg ggccgacaac catcgcccca
     1501 ctccctccga agatagggtg gggcgatggt ttgaaccgcc ccagcgagaa tgggccggga
     1561 ctcatgccgc cacaaccaac ttgcgactgc agaccatgga ccgtgacgga tccagtgggc
     1621 ccagcaggac tcgaacctgc gaccggccga ttatgagtcg gcagctctaa ccaactgagc
     1681 tatgggcccc ggtgttgccg tgccattgta cccgaccgac tccctcaccg gcgaggccgg
     1741 gcacccgcac ggggggcggc gaccgccgac acgaggtccg gagcggcggc cgcaccgcgt
     1801 cgtgcggggc cgggcgaccc gcggctgcac cgagtgaatg cgacgggtgc ggcacccctc
     1861 gcgtcgccgc ctccgacgcc gggcggttct cgcgtcgccg ccccggccgg ctgcagagcc
     1921 gcccccggcg gcccgcagag ccgcgctgtg gcgccggccg gccccctccg gccgcaatca
     1981 cgccctcgtc agcacgaaca ctccatgctc cgccaacacg ttgggccaga aacgaacccg
     2041 gtgcctggga cccagatgca cgctccgcac tatgcggcac ccctgctccc gagcgaacac
     2101 acggaagtcc tcgatactga gactccggat gttgggcgtg ttgtaccact cgtagggcag
     2161 tgccttcgtg accggggccc gccccttgaa cgtgaggtgg aagcgcga
//
LOCUS       DASRMJ010000023         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_436358, whole genome shotgun sequence.
ACCESSION   DASRMJ010000023 DASRMJ010000000
VERSION     DASRMJ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436358"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            497..826
                     /locus_tag="VFD74_00530"
     CDS             497..826
                     /locus_tag="VFD74_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HZK48076.1"
                     /translation="MYMIGIPFKTTDWSTMEPTEHVGVTGKAYWRTREFDGIRVRIVE
                     YTPGYKADHWCEKGHIVLCLEGELNTELKDGRVFVLKPGMSYQVENGVEPHRSYTEVG
                     APLFVVD"
     gene            893..1201
                     /locus_tag="VFD74_00535"
     CDS             893..1201
                     /locus_tag="VFD74_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HZK48077.1"
                     /translation="MLASFSVAPFGVGEGLGEFVAGMMPLIEASGLPFKLGAMQTTIE
                     GDEAEVMQLIMSCHRYMREKAPRVVTHITIDDRAGGTGRLEGKVHDVERILGRELRHE
                     "
     gene            complement(1221..1397)
                     /locus_tag="VFD74_00540"
                     /pseudo
     CDS             complement(1221..1397)
                     /locus_tag="VFD74_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1519..2778)
                     /locus_tag="VFD74_00545"
     CDS             complement(1519..2778)
                     /locus_tag="VFD74_00545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK48078.1"
                     /translation="MRDFEDSDLDQAVHLWEEVTIDGSRPLFRVTEVVAAVLAAEPAL
                     VAVVEGQVIGTVVSRVSGDRAWILRLAVSAAWHKRGVRSSLLDAIHRELLDKGVRQTT
                     AVFPAGEVEEQLFLDHGFTIRPEVTYFERFLPTQPAELNLLAELGGQLVPAGLWERLE
                     GMDREKQLIERRIILPLAKPILAEQHGVQVPKAIVLFGPPGTGKTTFAKGIASRVGWP
                     FIELFPSRLAAGGRDAQAHELRLFFRRLAEIDAAVVFIDEVEEIAQSRDDPPVSHAVT
                     NELLKAIPVFRGHPNRLLICATNSIRTLDSAFLRPGRFDLLLPIGPPDDQARWSIWGR
                     YVEGITAAEVDLDALVEASALFTPADIEFAARKAAQLAFERAVFEEAEARGGTEDFLQ
                     AISEVRPALSRGVVREFEEDIGDFARF"
     gene            3015..3131
                     /locus_tag="VFD74_00550"
                     /pseudo
     CDS             3015..3131
                     /locus_tag="VFD74_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
     gene            complement(3264..3752)
                     /locus_tag="VFD74_00555"
     CDS             complement(3264..3752)
                     /locus_tag="VFD74_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZK48079.1"
                     /translation="MSLLQRNGAGGPGAERAVGEGSAGAAGRDAPSGVPDPVLVEHAR
                     RRRFTAEYKLRILTEAEAATRPGEVGALLRREGLYSSHLTKWRRQRDEGALEALSLPR
                     GRKPAHPLEADNAKLRRRLERTEAELEKAHRVIEVQGNVSALLGELLEPRGAKPEKST
                     EQ"
BASE COUNT          655 a         1262 c         1306 g          655 t
ORIGIN      
        1 tccagggaga tggcccgcaa gaagactccc tgccggtgca gtggccgcgc tgtccacccc
       61 acccacagct ccagtccgat tggggtcctt tggcgtgctc agctttcgac cggggctggc
      121 tgttcagcat tcaaccatcc acaaccgatg atggcgcgga gtatcctttc gggaaccgga
      181 tggggcacga gaggacccag tgtcctccat cggcatccca cccgagttct ggctaggacg
      241 atccttcgag caggaggcaa gaccaccttg tcttggggcg acgtgcgtca cagggctatt
      301 ctcatggacg gggcgctggc cctgctactt ctggccgcct acctcgcgtg ggcagtgggc
      361 cgaccgccac ttgccgcgac aatcccgctc gccatcctgc agaccgctcc tttgctctgg
      421 cgacggcggc ctccggtggt actttcctgg aaatcaggcg aaagcagacc gggccaccct
      481 tgaacaggag gcgccgatgt acatgatcgg gatccccttc aagaccaccg actggtcaac
      541 catggagccg accgaacacg tgggtgtgac cggcaaagcc tactggcgga ctcgggagtt
      601 cgacggcatc cgagtgcgca tcgtggaata cacgccgggc tacaaggccg accactggtg
      661 cgagaaagga cacatcgtgc tctgcctcga gggcgagttg aataccgaac tcaaagacgg
      721 gcgcgtgttt gttctgaaac cgggcatgag ctaccaggtg gagaatggag tagagccgca
      781 tcgctcgtac acggaggttg gagccccgct cttcgtggtt gactgagcgc gatagggcct
      841 cgcggcggat gcgccgtagg cggcggcatt ccgagaggac tggaggtccg tcatgctggc
      901 cagtttctct gttgctccct ttggtgttgg agaaggactg ggcgagttcg tggcggggat
      961 gatgccgctg atcgaagcct ccgggcttcc ctttaagctg ggagccatgc agaccacgat
     1021 cgagggggac gaggccgagg tcatgcagct catcatgagc tgccaccgtt acatgcggga
     1081 aaaggcacca cgggtggtga ctcacatcac catcgacgac cgcgccggag gtaccggccg
     1141 gctcgagggc aaggtgcacg acgtggagag gatcctgggg agagagcttc gccacgaata
     1201 ggtgcctggc ggggcgcctg ctactccacg gcgtcacacc ccagatcgaa ctggaccccc
     1261 tgagccagcg gcagatccac cccgtagttg atggccgcag tctggcggcg ggcgtaggct
     1321 ttccaggcat cgcttccgct ttcgcggccg cccccggtct ccttctctcc gccgaaggcc
     1381 ccgcctatct ccgcccccgg gagcccgatc tgcggttggg acaatcgcac gttcgcgtgg
     1441 aaccgttgac acgagggaat cggagccccg gtggcggaag ctggcagtct gggttcgccc
     1501 gctcgcggcc gggccgcgtc agaaccgcgc gaaatccccg atgtcctctt cgaactcgcg
     1561 caccaccccc cgggaaaggg ccggccgcac ctccgagata gcctgcagga agtcctcggt
     1621 accccctctg gcctccgctt cctcgaacac ggcccgctcg aaggcgagtt gagcggcctt
     1681 gcgggcggcg aactcgatgt cggccggggt gaagagggcg ctcgcttcga ccagagcgtc
     1741 gagatcgacc tccgcggcgg tgataccttc gacgtagcgg ccccatatcg accagcgcgc
     1801 ctggtcatcg gggggaccga tcggcaacag gaggtcgaac cggccgggcc gcaggaacgc
     1861 cgagtccagg gtgcggatcg agttcgtagc gcagatgaga agtcgattgg ggtgaccccg
     1921 gaagaccggg atcgccttca gcagctcatt cgtcaccgcg tgggagacgg gcgggtcgtc
     1981 gcggctctgg gcaatctcct cgacctcgtc gatgaacacc acagccgcgt cgatctcggc
     2041 cagacggcgg aagaacagcc gcagctcgtg ggcctgagcg tcccgccctc cggcggccag
     2101 ccggctgggg aagagctcga tgaacggcca gcccacgcgc gaagcgatcc ccttggcgaa
     2161 cgttgtcttg ccggtgcctg gcggtccgaa gaggacgatc gccttgggta cctggacgcc
     2221 gtgctgttca gcgaggatcg gcttcgcgag gggcaggatg atccgccgtt cgatgagctg
     2281 cttctcgcgg tccatccctt cgagccgttc ccaaagaccg gccggcacca gctgcccacc
     2341 caactcggcg aggaggttga gctcggccgg ttgcgtgggg aggaaccgtt cgaagtaggt
     2401 gacctcaggg cggatggtga acccgtggtc caagaacagc tgctcctcga cctctccggc
     2461 cgggaacacg gccgtcgtct gccggacgcc cttgtcgagc agctcccggt ggatggcgtc
     2521 gagcagactg ctcctcaccc ccctcttgtg ccaggcggct gagacggcca gacggaggat
     2581 ccacgcccgg tcgccgctga cacggctgac cacggtgccg atcacctgcc cctccaccac
     2641 ggctaccagg gccggttcgg cggcgaggac ggcggcgaca acttccgtta cccgaaagag
     2701 cggccgcgag ccgtcgatgg tgacttcttc ccagaggtgg actgcctgat cgaggtcgct
     2761 atcctcgaag tcgcggatct gtcagatggg tggcatgagg gccccctcgg tctgcttcgt
     2821 cggcgaacgg ggagtctacc cctggggacg gcgaagagca aggggggcga acctgagcac
     2881 cgaagcagca ccttgcgtcg gagccccagg ctcagataga gtgtgacgca cgtttagcct
     2941 gacgtggaat cggttgcgcg cccaaggagg cagaccgcca cgtcttggtt cctcaagggc
     3001 gcaaggagcg agtcatgaac tgcttgatcc ttgcggccgg gcagggaagc cggctctctt
     3061 gccaccacga acccaaaccc ctcgttccgg ttggaggtct tcccctcctg gaacgggtga
     3121 tcctcgccgc tcgagccgcg gtcgcgggga gcggggctcc ggtggccggc ggcgacggta
     3181 gatggtggcc ggcgagacgc ctagggcgcg acaggcgggg cgagtgccca ccaggggggc
     3241 aagcttctcc acggtctctt ggatcattgc tcggtgctct tctcgggctt ggcgcccctg
     3301 ggctccaaga gctcgcccag aagcgcggag acgtttccct ggacctcgat cacccggtgg
     3361 gccttctcga gctcggcctc ggtgcgctcc aggcgccggc gcagcttggc gttatcggcc
     3421 tccaggggat gggccggttt gcgccctcgc ggcagggaaa gcgcctctag ggcaccctcg
     3481 tcgcgctgcc tgcgccactt ggtgaggtgt gaggaataga gaccctcgcg ccggagaagt
     3541 gcgcccacct cgcccggccg ggtggcggcc tccgcctcgg tgaggatgcg gagcttgtac
     3601 tcggccgtga aacggcgacg gcgggcgtgc tcgacgagca ccgggtcggg gactccggac
     3661 ggggcgtcgc gcccggccgc ccctgcgctt ccctcgccta cggctcgctc agctccgggg
     3721 ccaccggccc cattcctctg caacaacgac atcccacgtg ctccgatctc accctcatgg
     3781 ttcagtaaat cccgtgctcg gatcgtctca cgtaagcttg gcagagaggg aaatgcaggg
     3841 ttacgaaata gcaagctcat taccagttga tgctgtgc
//
LOCUS       DASRMJ010000024         7407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_75664, whole genome shotgun sequence.
ACCESSION   DASRMJ010000024 DASRMJ010000000
VERSION     DASRMJ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75664"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VFD74_00560"
     CDS             <1..1409
                     /locus_tag="VFD74_00560"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HZK48080.1"
                     /translation="IAASVVRRVLRRTSVDDRIAARIMGEERAGQANVERATAKVVFW
                     LIMVFVFVATFQALGLTLITQPLNNLLNELFAFLPRLLGAGILVVIAWGLASLLRILV
                     SRGLEVAKLDERVSRKADAGEEPRERRVPVARTLGDAVYWLVFLLFLPAILGALALEG
                     LLTPVQGMLDRFLTFLPNIASAALIALIGWFVARVVQRIVSNLLSAAGTDQLSDRVGL
                     SKALGSQKLSRLVGLVVYILILVPVLVAALDALKLEAVSTPASNMLNMFLEAIPAIFG
                     AALVVGLSYVVGRLVAGLVGNLLAGIGFDRILLRLKLTRGESQDPLWTPSAIVGHVVL
                     VVIMLFAATEAFALLGFEALSGLVSQFLFFAGHVAMGLAIFGLGLYLADVVARVIGAS
                     GVVQAGLLALVARVSVLLLAGAMALQQMGFANEIVVIGFGVIVGSVAVALAVAFGVGG
                     RHIAARELENWVNSWKSD"
     gene            1722..2420
                     /locus_tag="VFD74_00565"
     CDS             1722..2420
                     /locus_tag="VFD74_00565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK48081.1"
                     /translation="MKLRVGVVIPAHNEAEFITTVIHAARAVPEVDEILVVDDGSSDR
                     TAEVAAKQGARVVSHEENLGKGAAMTTGCRSSRSDVLIYLDGDLQNVTPEKIRRMIEP
                     FREGIDFVKTRFERRGGRVTQLTARPMLGHFFPEIEEQFDQPLSGQIGIKKTLMGQLE
                     LEEDMGVDLGLLIDAVESGAGVSEVCIGELEHDERELKDLQVMARAVVRVILDRAVRY
                     QRVDKAVEDIVEAG"
     gene            2473..4059
                     /locus_tag="VFD74_00570"
     CDS             2473..4059
                     /locus_tag="VFD74_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HZK48082.1"
                     /translation="MLKTMLDQTRHRGPDGAGLAVGLAVERAGDVAELAVDALSGLRG
                     LGHSRLKITGETGIQPLTGCGRGLVLAFNGEIWNHEPLREGLEATDHVFTTDSDGEAL
                     IHQLEDEMCNAGDLVKAVEAVLRGLDGEYAFAVYGGLHDEFVLARDVVGVKQLYYGET
                     AEHLAFCSEKKPLWDLSITPQRVLPGQIVKLAPDGSSGAWQFSVGGRVELARPGRQCE
                     TAQERGALEAYLKSLVEAVRKRVEGHDRVGIIFSGGVDSVLIARIAQRLGVDVRCYAG
                     GLPGSSDVDLSRVAADALGMPLAVSELDQDRIESELPLILGAIESANHLQVDVAIPIY
                     FAVKRAAQDGIRVMLTGQGADELFAGYQWYPEILAKSGAESLRDSLWDDLSNLYKDTL
                     EREDKITMFHSIELRVPYLDPSVISTAMRIAPTLKIRDGELKYLHRRLAEQVGVPHAI
                     AWRPKEAAQHGSNVHETLKTVLSRMEEGLDVPARHGRTEPDDASEELGSAYRHGGDAY
                     AGNGRYQKVLDSLGRAAGLA"
     gene            4056..4796
                     /locus_tag="VFD74_00575"
     CDS             4056..4796
                     /locus_tag="VFD74_00575"
                     /inference="COORDINATES: protein motif:HMM:NF016972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HZK48083.1"
                     /translation="MSRLSPREKRGLILATDLDGTLLGGSEDDRRRLTDAIEDEPSFQ
                     VIFLTGRNLETICPCLDDPHVPTPDYIVGDVGATVVSGKDLKPIQPLQNELDRAWPGR
                     TAVERALRPFPFLERQDVPQERRCSYRVTDERSITRELLLAVESIGCSALFSAGRYLD
                     VLAEGVSKGTTLLKLIDLQRLDSATVVVAGDSLNDLSMFETELKGVVVGNGEAGLKQA
                     VAEMSSVFVARRQGAGGILQGLEHHGTV"
     gene            complement(4926..5294)
                     /locus_tag="VFD74_00580"
     CDS             complement(4926..5294)
                     /locus_tag="VFD74_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48084.1"
                     /translation="MVEAEWLDRYVLLLAEYGALLRMRRFEVRPADDDHSLAWSRIFR
                     DGQEVESEKTAALLADDEEAGVHRNVCRQLLGGLRAWTLGDPGQADFFWAGKGPFDRT
                     PSFFRRQRRSLRTSRKRSED"
     gene            5555..7057
                     /locus_tag="VFD74_00585"
     CDS             5555..7057
                     /locus_tag="VFD74_00585"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HZK48085.1"
                     /translation="MRTPRFVAVSNRLPVKLTRDEDGTIRPLAEVGGLVSALAPVLSN
                     RGGQWVGWPGVEHTADLDHVLEEASRAAGYVLRPVYLARADAELYYNGFSNGIIWPLF
                     HDLPSRCNFTPEAWDAYQRANQSFATVVADSCAESEHIWVHDYHLMRVGRELREMGVT
                     APLGFFLHIPFPPLDMFLRLPWRAEVLLALLAYDLVGFQTLRDRRNFLYCVRHLLPGC
                     ATRGQGRVVTVDYEGREVRVGSFPIGIDYTDFRESAGSVAVRSAAEQLREQLGDRTLI
                     LGVDRLDYSKGIPEKLEAFRSALLRYPELQGKTTLVQIAVPSRLDVPEYQELRVRIER
                     LVGEINGQFTRGGWAPIQYLFRRVDRAELLAHYVAADVALITPLKDGMNLVAKEYCAC
                     SSDRPGVLILSEFAGAASQLHRWALTVNPHDVKEVAEAIYLGCTMTSSEKRTRMRHMR
                     RSLKAHDVYRWVDDFLSAAAGKSLQDFPFQEEYMPPLEDRTDRGDRPATS"
BASE COUNT         1278 a         2285 c         2455 g         1389 t
ORIGIN      
        1 tgatcgcggc ttcggtggtg cgaagggtcc tgcgccgcac ttccgtggat gatcgcattg
       61 ccgcccggat catgggggaa gagagggcgg ggcaggcgaa cgtagagcgg gctacggcga
      121 aggtcgtctt ctggctgata atggtcttcg tgttcgtagc gaccttccag gcactcgggc
      181 tcaccttgat cacgcagcct ctcaacaacc ttttgaacga gctgtttgcc ttcctccctc
      241 gtctcttggg cgcggggatc ttggttgtta tcgcctgggg gctcgcgagt ctgctgcgca
      301 tactcgtatc ccgtggtctc gaggtggcga agctcgatga aagggtgtcc cgaaaagcgg
      361 atgcaggcga ggagccccgg gagcggcggg tcccggtagc gcggaccctg ggtgatgccg
      421 tgtattggct cgtgtttctg ctcttcctgc ccgccattct gggtgcactt gctctggaag
      481 ggcttctgac gccggtccag ggcatgctgg acaggttctt gacgttcttg ccgaacatcg
      541 cgagcgcagc gctgatcgcg ttgatcggct ggttcgtggc ccgcgtggtg cagcggatag
      601 tatccaacct gctctcggcc gccggcaccg accagttgag cgaccgggtg ggcctgagca
      661 aagctctggg ttcgcagaag ctgtcccggc tggttggtct ggtcgtctac attctgatcc
      721 tcgtgccggt gctcgtcgcc gcgctggacg ccctcaaact cgaggccgtc tctacgccgg
      781 cgagcaatat gctgaacatg ttcctcgagg cgatcccggc tatcttcgga gccgcccttg
      841 tggtcgggct gtcctacgtg gtgggtcgct tggtggccgg cctggtcggc aacctgctgg
      901 cggggatcgg cttcgatcgg attctgctgc ggctgaagct gacccgtggg gaaagtcagg
      961 atccgctctg gactccatcc gccatcgtcg gtcatgtcgt gttggtggtg atcatgctgt
     1021 tcgcggccac cgaagccttc gccctgttgg gcttcgaggc actgtcgggc ctcgtgtcac
     1081 agttcctttt cttcgccgga cacgtggcta tgggcctcgc gatcttcggc ctcggcctgt
     1141 acctggccga cgtggtcgct cgggtcatag gagcgagcgg cgtggtgcag gccgggctcc
     1201 tcgcactggt cgctcgagtc tcggtactgc tgctggcggg agcgatggcc ctgcagcaga
     1261 tgggtttcgc caatgagatc gtggtgatcg gcttcggtgt gatcgtgggt tccgtggccg
     1321 tcgccctggc cgtggcgttc ggtgtcggtg gccgccacat cgccgcgcgg gagctggaga
     1381 attgggtgaa ctcgtggaag tccgactagg ggaggacacg agcgatcata ccggcccagg
     1441 tgatctcggt gctgtggtgc cgaggtctat gcggacccga ccgaactcta cccggagggc
     1501 ggcctcgtgg actgccgctc gcgcagctgc gagcgcgggc ggagctttct caagctcgag
     1561 cactacctgc cggccttcca caagaagcgt tgggcggcgg tcgggtgggc ggtcctttcg
     1621 ccggccagcc ccagcttctt ctccgccagg ccgccagacg caggcgccgg atagatgaca
     1681 gagaatgata tgtacagcac cccatcatga tcgagattca cttgaagctg agggtcggtg
     1741 tagtcatccc cgcccacaac gaagctgagt tcatcacgac tgtgatccac gccgcccgtg
     1801 cagtacccga agtcgacgag attttggtcg ttgacgacgg aagcagcgac cgtacggccg
     1861 aggttgcggc gaagcagggg gcgcgggtcg tctcgcatga agaaaacctc ggcaagggag
     1921 ctgccatgac aacgggctgc cgcagctcgc ggagcgacgt actgatctac ctcgacgggg
     1981 accttcagaa cgttacaccc gagaagatcc ggcgcatgat cgagcccttc agagagggca
     2041 tcgacttcgt caagacacgc ttcgaacggc gcggcggtcg agtcacgcag ctcacggccc
     2101 gtcccatgct cggccatttt ttcccggaga tcgaggagca attcgatcag ccgttgagcg
     2161 gacagatcgg catcaagaag acgctgatgg gccagctcga gttagaggag gacatgggtg
     2221 tagacctggg gctgctcatc gacgcggtag agagtggtgc aggtgttagc gaggtctgta
     2281 tcggcgagct cgaacacgac gaacgcgaac tcaaggacct ccaggtgatg gcgagagcgg
     2341 ttgtccgggt gattctggac cgggcggtgc gataccagcg tgtagacaaa gctgtcgaag
     2401 acatcgtgga agcgggttga tatgtgctcg attgttggct tggtgagcag aagcggtgcg
     2461 gacgtgtgtc cgttgctgaa aacaatgctg gaccagacgc gtcaccgcgg gccggacggg
     2521 gccggattgg ccgtgggcct cgcagtggag cgtgcgggtg atgttgccga gctcgcggtc
     2581 gatgcactga gcggactccg cggtcttggc cactccaggc tcaagatcac gggggagacc
     2641 ggcatccagc cattgaccgg ctgcgggcga ggtctggtgc tggctttcaa cggggagatc
     2701 tggaatcacg agccgctcag ggaggggctc gaggccaccg accacgtctt cacgacagac
     2761 tccgatggtg aagccctcat ccatcagctc gaggacgaga tgtgcaacgc aggcgacctg
     2821 gtcaaggctg tagaggccgt gctccgggga ctggacgggg aatacgcctt cgccgtctat
     2881 ggcggactcc acgacgagtt cgtcttggct cgtgacgtcg tgggcgtgaa gcaactctac
     2941 tatggagaga ccgccgagca tctggctttc tgctccgaga agaagccgtt gtgggacctc
     3001 tcgattaccc cccagcgcgt gcttccaggg cagatcgtca agctcgcgcc ggatggttca
     3061 tcgggcgctt ggcagttctc ggtcggtggt cgcgtcgagc tcgcgcgtcc gggccggcag
     3121 tgcgaaacgg cccaggagcg aggtgcgctc gaggcctacc tgaagtctct ggtcgaggcc
     3181 gttaggaagc gcgttgaggg tcatgatcgg gtcggtatca tcttctcggg tggcgtcgat
     3241 tcggtcctga tcgcccgcat tgctcaacgc ctcggtgtcg acgtccgctg ctatgccggc
     3301 ggcctcccgg gctccagcga cgtcgacctc tctcgtgtgg ctgccgacgc gctgggaatg
     3361 cctctggccg tctccgagct ggaccaagac cgcatcgaga gtgagcttcc cttgatcttg
     3421 ggcgccatcg agtcggccaa tcacctccag gtcgatgtcg caatcccgat ctacttcgcc
     3481 gtgaagcgtg ccgcccagga tggcatccgc gtcatgctga cgggccaggg agccgacgag
     3541 ctctttgccg gatatcagtg gtatccggag atcctggcaa agtccggcgc cgagagcctg
     3601 cgcgacagcc tgtgggacga cttgagcaat ctctacaaag acaccctgga gcgggaagac
     3661 aagatcacga tgttccatag catcgagctc cgcgtcccgt acctggaccc ctcggtgatt
     3721 agcacggcca tgcggatcgc tcccacgctc aagatccggg atggtgagct gaagtacctt
     3781 caccgcaggc tggcggagca ggtgggtgtt ccgcatgcca tcgcgtggcg tcccaaggag
     3841 gcggcccagc acggctcgaa cgttcacgag actttgaaga cggtcctttc ccggatggaa
     3901 gaggggcttg acgtcccggc gcgacacggg cggacggagc ccgacgacgc cagtgaggaa
     3961 ctggggagcg cctaccgtca tggcggagat gcctatgccg gaaacggccg ctatcagaag
     4021 gtcctggatt ccctcggtcg cgcagcgggc ctggcatgag ccgcttgtcg cccagggaaa
     4081 agcgtggcct catattggcc accgatctcg acggcactct gcttggtggc tcggaagacg
     4141 atcgaaggcg gctgacggac gccatcgaag acgaaccgag tttccaggtc atcttcttga
     4201 ccggccggaa tctcgagact atttgcccct gcctcgacga tccccatgtg ccgactcctg
     4261 attacatcgt cggcgatgtc ggtgccaccg tcgtttccgg caaggatctg aagccgatcc
     4321 agcccctaca gaacgagctc gatcgggcct ggcccgggcg caccgccgtc gagcgggcgc
     4381 tcaggccttt cccattcctg gagcggcagg acgtgccgca ggagcggcgc tgttcctacc
     4441 gcgtaaccga tgagcgctca atcacccgcg agctactcct agccgtcgag tccatcggct
     4501 gctccgccct tttttcggcg ggccgctatc tcgacgtctt ggccgagggg gtctcgaagg
     4561 gcacgaccct tctcaagcta attgacctcc agaggctcga ttcggccacg gttgtcgtgg
     4621 cgggggactc gctcaacgat ctctccatgt tcgagaccga actcaagggc gtggttgtgg
     4681 gcaacggcga agccggactc aagcaagcgg tcgcggagat gagctccgtc ttcgtggctc
     4741 gacgtcaggg agccggagga atcctgcaag gactcgagca tcatggaaca gtatgagaca
     4801 gctcggacgg aagtccaacg cgggggctga gcatcggggt ctccaacttc tggaagcggc
     4861 gaggttcgcg tgttccccgg cgaaacactc ccaagcggga acgaagtggt cgttcgcaag
     4921 atcgatcagt cctctgaccg cttcctcgaa gttctcaagg agcgccgctg cctccggaaa
     4981 aaggagggcg tgcgatcgaa cggtcccttg cccgcccaga agaagtcggc ctggcccggg
     5041 tcgccgagcg tccacgcccg gagcccgccg aggagttgcc ggcagacgtt ccggtgaacg
     5101 ccggcctcct cgtcatcggc cagcagggca gcggtcttct cggactccac ctcctgcccg
     5161 tcccggaaga tgcgcgacca ggccagggag tggtcatcat cagccgggcg cacctcgaac
     5221 ctgcgcatgc gcaagagcgc cccgtactcc gccagcagaa gcacgtaccg gtcgagccac
     5281 tcggcctcca ccacgaagtg atagcgacgg gtcgggcagc gtctccgtgc gcgggcttcg
     5341 cctccgtgtg acggtgccta ctggaaccca ggggctacgc cccccaacgg agttcgaaga
     5401 gatgtactat cgttaacaat acatggtcga ggatgaggaa ctgaagcagc tccagtctcc
     5461 ggcgaacccc gcggttcaat gtggttgctg ccaatttcgt gtctcatgat cggcccgcca
     5521 cgaaacgacc acgttaccca ggggaggtgg ggccgtgaga accccccgct ttgtagctgt
     5581 ctcaaaccgg ctcccggtca agctgacccg ggacgaagac ggcacgatcc gccccttggc
     5641 ggaagtcggt ggcctggtat ctgcgttggc gcccgtgctc agcaaccgcg gcgggcaatg
     5701 ggtgggctgg cccggggtgg aacacaccgc cgacctcgat cacgtcctgg aggaggcgtc
     5761 gcgggcggcg ggctacgtgc tgcggccggt gtatctggca agagcggacg ctgagctcta
     5821 ctacaacggg ttctcgaatg ggatcatctg gcctctcttc cacgatcttc cttcacggtg
     5881 taacttcacc cccgaagcct gggacgccta ccaacgggcc aaccagagct tcgccacggt
     5941 cgtggccgat agctgcgccg agagcgagca catctgggta cacgactacc acctgatgcg
     6001 cgtcggccgt gaactccgcg agatgggtgt caccgcaccc ttggggttct tccttcacat
     6061 tcccttcccg ccgctcgaca tgttcttgcg tctgccctgg cgcgccgagg tgctgctcgc
     6121 cctcctcgcc tacgatctgg tcggcttcca gaccctccgg gaccggcgca acttcctcta
     6181 ctgtgtccgc cacttgctcc caggatgcgc cacgcgcggc cagggcagag tggtgacagt
     6241 cgactacgag gggcgcgaag tgcgggtggg cagcttcccc atcggcatcg actacaccga
     6301 ctttcgggag agcgccggta gtgtcgcagt gcggagcgca gcggagcagc tgcgggaaca
     6361 gctcggtgat cgaacgctca tcctgggcgt ggaccggctc gactacagca agggcatccc
     6421 cgagaagctc gaggccttcc ggagcgcact gctgcgctac ccggagctac agggaaagac
     6481 tacgctggtg cagatagccg tccccagtcg attggacgtc ccggagtacc aggaactccg
     6541 ggtccgcatc gagcgcctgg tgggcgagat caacggtcag ttcacccgcg gggggtgggc
     6601 tcccatccag tacctgtttc gcagggttga ccgcgcagag ctcctcgccc actatgtcgc
     6661 ggccgatgtg gcgctgataa ccccgctcaa ggatgggatg aatctcgtgg ccaaggagta
     6721 ctgcgcctgc agcagcgacc gccccggcgt attgatcctc agtgagttcg ccggggccgc
     6781 gtcgcagctg caccgctggg cgctcaccgt gaacccgcac gacgtcaagg aggtggctga
     6841 agccatctat ctcggctgca cgatgacgag cagcgagaag cgcacccgta tgcggcacat
     6901 gcgccgcagc ctgaaggcac acgacgtcta ccggtgggtc gatgatttct tgagcgcggc
     6961 cgccggcaag agcctgcagg acttcccctt ccaggaagaa tacatgcccc ctctggagga
     7021 ccgcaccgac aggggcgacc gccctgcgac atcctgaacg tctcgatcac agaaatgggg
     7081 ccgcaggatc tggcgggggg gcggttgcga caacgggcac cgacgccgcc gcccgagcag
     7141 cacacggtcc ccttcgcgta ctcgccgcct cacccggaca ttggcccggt atttgaggtg
     7201 gccgtgaacc gctccggttt cggcagagac tccgctgttt gtgcccttgg caatctcggg
     7261 tgaccgccac cacgatggaa caatggtgat gggtcatcca aaggcggggt ggcggaagcg
     7321 tgaggggtac atttattccg ttcacgccgc ttcccggtga tactcgttgg tcagtccgcc
     7381 gagccggctc gtccgagcta cctcacc
//
LOCUS       DASRMJ010000025         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_182426, whole genome shotgun sequence.
ACCESSION   DASRMJ010000025 DASRMJ010000000
VERSION     DASRMJ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182426"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="VFD74_00590"
     CDS             <1..1767
                     /locus_tag="VFD74_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48086.1"
                     /translation="PQVDYRDSLRVRLLDSNEAPITAWAKGAVEYTTTVLVVGSGNSD
                     LLDFISENTGWLKVSQTSAVPRAVSGLSAYDLVIFASASAGDLATSGDLATSGDLTGA
                     DSALRDWVARGGRLIVAAPSSLPAVAMEAFPDARFGDQRTLDVPAGLRMRGSGGMISD
                     ELARLGAGTVITPVGVSSAPYLAATEDGTVVYVSAPFGRGSVALLGLSPNGATRDEAG
                     APQFFFWESVVMADRLSSAYMGSWSPAVDPPHTFVTGRVPSLLATLLGAVIYTLAVGL
                     GLFVLLRRVGRRDAVWWVVPSVVLVATAAVLAAGLWARGSNPEVLVRQTISVDATTGL
                     RTAATEVGVYAPFGAEYGVDLSPDLQPGGLESTSGLFARPYTIRDRTAVGGRIAYEGI
                     SIAPGAGRSVSVWEDAEVVSGASPATPFALSKAMAGDGTRWTVTNTGAVPLRRLILVA
                     DTGVYTLPDLPPGASTAFVSSVETSGLVTGEGAVAVTEEPPPPSSHDYLLWEVQARLA
                     AEWRSVGPWSEGSIRSPEVATSGVMQVKSPALDALPQGPVRLIAFAEGTAAEGMAEGL
                     TVTGLPSRTERRGVVVYEVTLR"
     gene            1764..2720
                     /locus_tag="VFD74_00595"
     CDS             1764..2720
                     /locus_tag="VFD74_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48087.1"
                     /translation="MSDASVAGGPLLRVRGLVKRYGRTVALSGVDLDVAAGTIHGLVG
                     TNGAGKTTLLRILATVLGADGGSVVLGETDLLAHPREARSLFGFMPDFFGVYDDLTVE
                     EYLDFFGAAYGLERSARARRLPDLLTMTHLEGKAASDVQDLSRGMQQRLCLARALVHE
                     PPLLLLDEPASGLDPRGRAELRDILKGLAANGVAVLVSSHILSELADVCSAVTIIEQG
                     RVVASGSVEEIAGVARKVRTLLARLSGDAGPWLAVIAAQPGVVRVDPTVSGTVEIVFE
                     GDTAAQVALVAGLVGAGVPLAALEAPRALEEAYFESTQGLVT"
     gene            2758..>3522
                     /locus_tag="VFD74_00600"
     CDS             2758..>3522
                     /locus_tag="VFD74_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK48088.1"
                     /translation="MMLEGATALLRRELKGQVRGRRAPATLTAYLALMVIVLGGVLLV
                     SETVAGSGTTADSGQVLFIALAMTQTALILLLGPVFSAGALAGERERRTFDTLVCSRL
                     TSGGIIVGKVAGALVFQLLLATSSLPLMALAYVYGGVGPGTLFAVYGWDILLMVWVAS
                     LGTAVSSLVRRTLWAAVITYVLVFLSVFMTGFLEIVVQAVLADSGRSQPSFYFIYLNP
                     FAGLWELVIPGAREGLANGENVGSFVVWGGVWHAGVI"
BASE COUNT          454 a         1160 c         1259 g          649 t
ORIGIN      
        1 ccccaggtgg actaccggga ttcgctgcgg gtccgcctcc tcgactccaa cgaggccccc
       61 atcaccgcct gggcgaaggg ggccgtcgag tacacgacca cggtgttggt tgtgggaagc
      121 gggaactccg atctcctcga cttcatctcc gagaacaccg gttggctgaa ggtgagccag
      181 acttctgcgg tcccccgtgc ggtgtccggt ctcagcgcct acgatctcgt catcttcgcg
      241 tcggcgagtg cgggcgacct cgcaacttcg ggcgacctcg caacttcggg cgacctcacc
      301 ggtgcggact cggcgctgcg cgactgggtt gcccgcggcg gccggctcat cgtggctgcg
      361 ccctcgtcgc ttccggctgt cgcgatggaa gcatttccag acgctcgatt cggcgatcaa
      421 cggacactcg acgtccccgc cgggctcagg atgcgtggga gtgggggcat gatctctgac
      481 gaactggccc gattgggggc gggcacggtc atcacgcccg tgggggtgag tagtgcaccc
      541 tacctcgcgg cgaccgagga cggcaccgtc gtgtacgtct cggccccgtt cggccgaggc
      601 tcggtagctc ttctcggcct ttctccgaac ggggcgacac gtgatgaggc cggcgctccg
      661 cagttcttct tctgggagag cgtggtcatg gcggatcggc tgtcctcggc gtacatgggc
      721 tcgtggtccc ctgcggtcga tccgccgcac accttcgtga cgggacgtgt cccttccctc
      781 ctcgcgaccc tgctgggcgc ggtgatctac acgctcgcag tggggctggg actgtttgtc
      841 ctgctccgcc gcgtgggacg tcgcgacgcc gtgtggtggg tcgtgccgag cgtcgtcttg
      901 gtggcaaccg cggctgtgct ggcggcgggt ctctgggccc ggggatccaa tcccgaggtg
      961 ctcgtccgac agaccatatc ggtcgatgcg accacaggcc tccgcaccgc tgcgacggag
     1021 gtgggtgtat acgccccgtt tggagcggaa tacggcgtcg acctgagccc cgacttgcag
     1081 cccggaggcc tcgagagtac ttccggactg ttcgcgcgac cctacaccat ccgagaccgg
     1141 accgctgttg gtgggcggat cgcgtatgag ggtatctcga tcgcccccgg agccgggcgc
     1201 tctgtgagcg tgtgggagga tgccgaagtc gtttcgggcg catctccagc gaccccgttt
     1261 gccctgtcca aggccatggc tggcgacggc acgcgttgga ccgtcacgaa cacgggcgcc
     1321 gtgcctttgc ggcgcctgat tctcgttgcg gataccggcg tctacacgct gcccgacctg
     1381 ccgccgggcg catccaccgc gttcgtctcg tccgtggaga cgagcggact cgtcaccggc
     1441 gaaggtgcgg tggcggtcac agaggagccg ccacccccga gctcccatga ctatctgctg
     1501 tgggaagtcc aagctcgtct ggcggcggag tggaggtcgg tggggccgtg gtcggaggga
     1561 agtatccgga gccccgaggt ggctacgtcc ggggtgatgc aggtgaagag tccggctctc
     1621 gacgcgctgc ctcagggtcc ggtgaggctg atcgccttcg cggaaggcac ggccgccgag
     1681 ggcatggccg agggcctgac ggtgaccggc ctgccttccc gcaccgagag gcgtggcgtc
     1741 gtggtctacg aggtgaccct gcgatgagcg acgcgtctgt cgccggcgga ccgctgctcc
     1801 gcgtccgcgg cctggtgaag cggtatggcc gcaccgtggc cctttccggg gtcgacctcg
     1861 acgtcgcggc gggcaccatc catggcttgg tcggcaccaa cggggccggc aagaccacgc
     1921 tcctgcgcat actcgccacc gtgcttgggg ccgacggagg ctcggttgtc ctgggagaga
     1981 ccgacctgct ggcccacccg cgggaggccc gctccctgtt cggattcatg ccggacttct
     2041 tcggggtgta cgacgatctc accgtcgaag agtacctgga tttcttcggc gcggcctacg
     2101 ggctggagcg gtcggcgcgg gcgcggcggt tgcccgatct gctgaccatg actcacctgg
     2161 agggcaaggc ggcttccgac gtgcaggacc tctcccgcgg catgcagcag cggctgtgct
     2221 tggctcgggc gttggtccac gaacctcccc tcctcctact cgacgagccg gccagcggac
     2281 tcgaccctcg cggcagggcc gaattgcggg acatcctcaa gggcctagcc gcgaacggtg
     2341 tcgctgtgct cgtctcgtcc cacatccttt cggagctcgc cgacgtgtgc tcggcggtga
     2401 cgatcatcga gcagggtcgg gtggtcgcgt ccgggtcggt ggaggagatc gccggagtcg
     2461 cccgcaaggt gaggacgctg ctcgcccgac tgtccggcga cgcgggcccc tggctggcgg
     2521 tgatcgccgc acagccgggc gtcgtgcgcg tcgatccgac ggtctcgggc accgtggaga
     2581 tcgtcttcga gggcgacacc gcggcgcagg tcgcgctggt ggcgggtctg gttggtgcgg
     2641 gcgtaccgct ggcggccctc gaagctccgc gggcgctgga agaggcctac ttcgagtcca
     2701 cgcaagggtt ggtgacgtga tgacctgccg gcggcacggg accagcgggg ggcgggcatg
     2761 atgctcgagg gcgcgaccgc gctgctcagg cgcgaactca aaggtcaggt gcggggccgg
     2821 cgggctccgg caaccctcac cgcctacctc gccctcatgg tgatcgtcct tggcggagtg
     2881 ctcctggtga gcgagaccgt ggcgggatcg ggaaccaccg ccgactcagg ccaggtgttg
     2941 ttcatcgccc tcgccatgac gcagaccgcc ctcatcctgc tactcgggcc cgtcttctcg
     3001 gccggcgccc tcgccggcga acgagagagg cgcacgttcg acaccctggt ctgcagccgc
     3061 ctcacgtccg gcggcatcat cgtcggcaag gtggccgggg cgttggtgtt ccagctgctg
     3121 ctggcgacct cgtcgctccc gctgatggcc ttggcctacg tgtatggggg cgtgggcccc
     3181 gggacgctgt tcgcggtgta cggctgggac atcttgctca tggtctgggt ggcgtctctc
     3241 ggcaccgccg tctcgtccct ggtgcgccga acactgtggg cggcggtgat cacctacgtc
     3301 ttggtcttcc tctcggtctt catgaccggg ttcctcgaga tcgtggtgca ggcggtcttg
     3361 gcggacagcg ggcgttccca gccgtccttc tacttcatct acctcaatcc cttcgccggc
     3421 ctgtgggagc tggttatccc cggggcgcgc gaggggctgg cgaatggaga gaacgtcggc
     3481 tccttcgtcg tctggggcgg agtctggcac gctggggtaa tc
//
LOCUS       DASRMJ010000026         7914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_326972, whole genome shotgun sequence.
ACCESSION   DASRMJ010000026 DASRMJ010000000
VERSION     DASRMJ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326972"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..504)
                     /locus_tag="VFD74_00605"
     CDS             complement(79..504)
                     /locus_tag="VFD74_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZK48089.1"
                     /translation="MFRRILVPSDGSEHALKAVRVAVDLAKEFDSEVVLLAVVPVPQT
                     LVMVAGISEHVIDDYIERAGRDALQSALDVFEEAGLGVEVKIEVGSAGEEILRQAQEA
                     GVDVIVMGKRGLGGVKGLLLGSVSNRVTHGAPIPVLLIP"
     gene            complement(562..634)
                     /locus_tag="VFD74_00610"
     tRNA            complement(562..634)
                     /locus_tag="VFD74_00610"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(599..601),aa:Ala,seq:tgc)
     gene            complement(856..929)
                     /locus_tag="VFD74_00615"
     tRNA            complement(856..929)
                     /locus_tag="VFD74_00615"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(893..895),aa:Ile,seq:gat)
     gene            1121..1606
                     /locus_tag="VFD74_00620"
     CDS             1121..1606
                     /locus_tag="VFD74_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HZK48090.1"
                     /translation="MDGYEKMSVYGVSFDVASKQPIVLLKSERTNRFLPIWIGHPEAA
                     AILMRLQEAELPRPMTHDLFMTVLQNLSTSLEHVLVTEFRDNTFYAVLHLLSGEHEID
                     IDSRPSDAIALAVRTAAPIFAANELLEANGIEMEQEPEDLEGVVSEFKEFLDAVSPED
                     F"
     gene            complement(1747..2037)
                     /locus_tag="VFD74_00625"
     CDS             complement(1747..2037)
                     /locus_tag="VFD74_00625"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZK48091.1"
                     /translation="MSTPTGDPVVRQYREQISDNDLKIVEALNKRIQLVEKLKSYKET
                     QGMEFFDPQQEDWVFTYLTRSNRGPLSPEGLREIFAVVLEVVKREAAVRSGD"
     gene            2180..2524
                     /gene="trxA"
                     /locus_tag="VFD74_00630"
     CDS             2180..2524
                     /gene="trxA"
                     /locus_tag="VFD74_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZK48092.1"
                     /translation="MGDSTMEITEDNFEAKVMQAAEPVLVDFWAPWCGPCRTMGPILE
                     ELGAEHQGTLAVGKLNVDDHQGLAARFNVLSIPTLLLFKDGEIVKKLIGALPKARLFE
                     ELAPWLGGGGDV"
     gene            2517..2744
                     /locus_tag="VFD74_00635"
     CDS             2517..2744
                     /locus_tag="VFD74_00635"
                     /inference="COORDINATES: protein motif:HMM:NF021636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HZK48093.1"
                     /translation="MCEAHAYMVSATGQDRIMENVVRIGYDEDGTLVLSDLFGDELRV
                     AARIREIALLEHKIYLESTGPDQASAGQAST"
     gene            complement(2729..4072)
                     /locus_tag="VFD74_00640"
     CDS             complement(2729..4072)
                     /locus_tag="VFD74_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48094.1"
                     /translation="MSAARILLAPTHRTGVALAITAALAEVMGQQERQVRYHQLGATS
                     PTAVWDRWEGSSFLDPALYGLETMLELYDSMVHGADLSLVATNRGLLDESAEGGWTPA
                     GVAQALECPVVLVVDCRGWAKGMAALVEGFRARSGDNAPAGIILTGVQDRSQREALRR
                     ALDRLGIPVVGCMYAGDGPGWDSVAPGAWSLPLGLDVVESVHRQVDASGLERLAGQRG
                     FFPPLAAGHGRVNGGDGPLIMVAGGRGFTPWSRDSIELLRAAGARIKRLDLARDESLP
                     PETAGVVLAGHLWVEALPELAANYQLMRELRVCAGEGMPMLALGGGMLYLLRRVQDDR
                     GRSFDLAGLLPAEGEMIGDLDEATYLDVEVKRDCLLFAEGERMKGWVSTDAEIMEAPA
                     SRSFPFTVSAAGWESPQSEGALARNLLCSRVLVHLASVPQSARRFVAACERYVEA"
     gene            4145..6004
                     /locus_tag="VFD74_00645"
     CDS             4145..6004
                     /locus_tag="VFD74_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK48095.1"
                     /translation="MALIARIRSTFGRLDDLIIARSILTGSLALASLLFAQTKSGGDL
                     DWWMALPLALVALANLPLFVLSARGHRVGVSWAVVVVDTCLITYLVHVTSGAQGAVAV
                     FYVWPIIAANLLLGARAGYLTAAVTAALYVGLAAAEAGGWEPRALLEGRGLSVADGLD
                     GVLVRASAFLLIALLSGLVSNALLDSNARLSQAKADAERDLDRTKLLNRRLTILDEVG
                     RILGRIQDLDILLPRALGRVAGFLGVEAGMIVLFGKDRSDIQIAARQSLSEATARDLL
                     AGDLPVSLDANRGAVLGSADGRSARALRTLQPLGYADFLAAPLRLGPEYVGNLYLFTT
                     APNPFRRGDVALMDSLCSQLAIAVKNVAFTRELKDANDELVNLDQLKSDFLATMSHEL
                     RTPLTSIIGYSDMLLSGMTGEVTDKQRDFLRSILNSGEALLDLINDILDLTKIEAGKL
                     ELSLEPVNLRTALASVLSVVKPRAKEKNLQISTFLPNDLPPLWADSGKLNQVLLNLLT
                     NAIEYTPETGRIGIEARPTPDGLVEIRVNDTGVGIEPADLARIFERFTQVDNTSTRSQ
                     GGTGLGLAITRDLIELHQGTITVKSQPGRGSQFIFTIPQAGAQKEPRSRARLA"
     gene            6015..6374
                     /locus_tag="VFD74_00650"
     CDS             6015..6374
                     /locus_tag="VFD74_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK48096.1"
                     /translation="MARIVVADDDPDILNIVAMSLEAMGHEVHRATNGREAVALTKAT
                     APDLVVMDLMMPEMNGYEATEALKADAETASVPVLALTAKAMRGDEERGRQAGVDAYV
                     TKPFRISQVVEVVDRLL"
     gene            complement(6544..7227)
                     /locus_tag="VFD74_00655"
     CDS             complement(6544..7227)
                     /locus_tag="VFD74_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HZK48097.1"
                     /translation="MFPFFDWTFILLLPALALSLYAQWKVKTTFAHYSKVPSGRGLTG
                     AAGARLLLDAGGLSHVPIEPVQGRLSDHYDPRTKVLRLSPDVAGRDSLAALGVAAHEV
                     GHALQDAEGYAPMRVRASLVPAASLGSNLGMILFIVGLFIGRNPLLMNAGILLFSAAV
                     LFTVVTLPVEFNASRRALALLSSRGLLVSTEVDGARKVLSAAGLTYVAAALMAVAQLA
                     RLLLISRNR"
     gene            7397..>7914
                     /locus_tag="VFD74_00660"
     CDS             7397..>7914
                     /locus_tag="VFD74_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HZK48098.1"
                     /translation="MPVRPGNGTGRLGDILVERKLVTQDNLERALSYQKESGSRLGEA
                     LIKLGYISDIHLAEALAAQKKLPIIALGEVFPNPRAASLLTEKFIRARRVMPVDFNQD
                     ALILAMVDPLDVVTLDDVRVITGLEVEPVVATASGFADTLEYVFSGRGVLDASERDEP
                     RDSRQVAEERRNA"
BASE COUNT         1396 a         2678 c         2599 g         1241 t
ORIGIN      
        1 cgatcaggta gcccgcgcct ctccccgcag acgccgcccg ccgcccgcag cacgcggcgc
       61 ggcgcggcgc gaccccgctt acgggatgag cagcaccggg atgggcgctc cgtgcgtaac
      121 acgattgctc acgctgccga ggagcaggcc cttgaccccg ccgaggccac gcttgcccat
      181 gacgatcacg tcgacgccgg cctcctgagc ctgccgaagg atctcctccc cggctgagcc
      241 cacctcgatc ttgacctcga cacccaggcc ggcttcctcg aacacatcga gcgccgactg
      301 cagggcgtcg cgcccggcgc gttcgatgta gtcatcgatc acgtgttcgc ttatgccggc
      361 gaccatgacg agagtctggg gcaccggcac caccgcgagg agcaccacct cggagtcgaa
      421 ctccttggcc aggtccaccg ccacccgcac ggctttcaag gcgtgctcgg atccatcgga
      481 cggcaccagg atacgacgga acacggcgtg acctcccttg tgcgcggaac cgatcgcgac
      541 catcggtcgc ggcacatcga gtggaggata ccgggatcga accggcgacc tccgccttgc
      601 aaaggcggcg ctctcccagc tgagctaatc ccccgcgaaa cagcacttcg gcgggtcgtg
      661 cgtggcgtcg gaacagcaga ctaccctgcc cccgaaccat agcccagagc cgctctggct
      721 gcaagcgagg cggcgcgacg agggcggcgg ctgcgtcgag gcggtacggc gtcgaggtgg
      781 tgccgcgtcg gggtgccagg gcggctcgaa gacggctcga aggcagcgcg aggacggcgc
      841 gggcgggcgg cgaagtgggc ctgcgtggag tcgaaccacg gacctcatcc ttatcagaga
      901 tgcgctctaa ccaactgagc tacaggcccc ctcgaacgga tgagtagttt accctcaatc
      961 aggcctttgt caagccgccg cccggatgcc accgacgtgc gcaggagcgg cccgcgtccc
     1021 cgcgcgacgg cgccccgtcg gtcgtaaagg tgggtggcac ggggcttcgg catgagctaa
     1081 gctaggttga gagtcgacct cgtggggtga gagaagaacg gtggacggct acgagaagat
     1141 gtcggtctat ggcgtgagct tcgatgtcgc cagcaagcag ccgatcgtgc tgctcaagtc
     1201 cgagcgcacc aatcgattcc tgcccatctg gatcgggcat cccgaggccg cggccatcct
     1261 catgcgcctt caggaagcgg agctccccag gcccatgacg cacgatctgt tcatgaccgt
     1321 gctccagaac ctctcgactt cgttggaaca cgtcttggtg accgagttcc gggacaacac
     1381 cttctacgcg gtgctgcacc tgctctcggg cgaacacgag atcgacatcg actcccgccc
     1441 ttcggacgcg atcgcccttg ccgtgcgcac ggcggctccc attttcgcgg cgaacgaact
     1501 cctcgaagcc aacggcatcg agatggagca ggagccggaa gacctcgagg gcgtcgtgtc
     1561 cgagttcaag gagttcctcg acgccgtctc tcccgaggac ttctgagtcc accgcgccgt
     1621 ctgccggcgg ccggcgggcg gcagctgggc gccatgtgag cgcgagtgga cggcgcggct
     1681 ggcgggcggc ggctggcggg cggcggtccc gtgcccggcg ccccatgccc agcgcccggc
     1741 gggcggtcag tcgccggagc ggaccgcggc ctctcgcttc acgacttcga ggaccaccgc
     1801 gaagatctcg cgcagtccct ccggactgag cggtccgcgg ttcgagcgcg tgagataggt
     1861 gaacacccaa tcctcttgct gcggatcgaa gaactccatc ccctgggtct ccttgtagct
     1921 cttgagcttc tccacgagtt ggatccgctt gttgagagcc tccacgatct tgagatcgtt
     1981 gtcggagatc tgctcccggt attgtctgac gaccgggtcg ccggtggggg tgctcactct
     2041 actgctcctt cgtgggctcg ggtgcgcctg ccgtgccggg gcgcctcggg tggacgatgc
     2101 aggcgtatag gctaccacct ctgcgcagaa tgggcctcaa ggcctggtgg tatagtggtc
     2161 gaagggaggt tgagacatcg tgggagattc gaccatggag ataaccgagg acaacttcga
     2221 ggccaaggtc atgcaggcgg cggaacccgt gttggtggat ttctgggcgc cgtggtgcgg
     2281 gccgtgtcga accatgggcc cgatcctcga ggaactcggc gccgaacacc aggggacgct
     2341 ggcggtgggc aagctcaacg tcgacgacca ccaggggctc gccgcccgct tcaacgtgct
     2401 ctcgatcccc acgctactgc tcttcaagga cggcgagatc gttaagaagc taatcggcgc
     2461 ccttcccaag gcccgactgt tcgaggaact ggcgccgtgg ctgggtgggg gcggcgatgt
     2521 gtgaggccca cgcctacatg gtgagcgcca ccggccagga caggatcatg gagaacgtcg
     2581 tgcgcatcgg ctacgacgag gacggcacgc ttgtgctctc cgacctgttc ggcgacgagt
     2641 tgcgggtcgc cgcgcgcatc cgcgagatag cactgctcga gcacaagatc tacctggaga
     2701 gcaccggtcc ggaccaggcc tcggcgggtc aggcctcgac gtagcgctca caggccgcga
     2761 cgaagcggcg cgcgctctgc ggaaccgaag ccaggtgcac tagcacacgc gagcacagca
     2821 ggttcctcgc gagcgccccc tccgactgcg gcgactccca gcccgccgcc gataccgtga
     2881 aggggaagct tcggctcgcc ggcgcctcca tgatctcggc gtcggtggag acccacccct
     2941 tcatgcgctc cccctcggcg aagaggaggc agtcgcgctt gacctcgacg tcgagatagg
     3001 tggcctcatc caagtcgccg atcatctcgc cttcggccgg cagcagcccc gcgaggtcga
     3061 acgaccggcc gcggtcgtcc tggacccgcc gcagcaggta gagcatccca ccgcccaagg
     3121 ccagcatggg cataccctca cccgcacaca cgcgcagctc gcgcatcagc tggtagttcg
     3181 ccgccaactc gggcagcgct tcgacccaga ggtgccccgc caacacgacg ccggcggtct
     3241 cgggcggcag ggactcgtcg cgcgcaaggt cgagacgctt gatcctggcg ccggcggccc
     3301 gcagcaactc gatcgagtcc cggctccacg gcgtgaagcc acggccgccg gccaccatga
     3361 taaggggtcc gtcgccgccg ttcaccctac cgtgccccgc tgcgagcggg gggaagaaac
     3421 cccgctgtcc ggccagacgc tcgagaccgg acgcgtccac ctgccggtgc accgactcga
     3481 ccacgtcgag gcccagcggc agactccacg caccgggagc gacggagtcc caaccaggcc
     3541 cgtcgccggc gtacatgcat cccaccacag ggatcccaag acggtcgagc gcgcgtcgaa
     3601 gggcctcgcg ttgagagcgg tcctgcactc ccgtgaggat gatgcccgct ggcgcgttgt
     3661 ctcccgaccg ggcgcggaac ccctccacca aagccgccat gcccttcgcc cagccgcgac
     3721 agtcgaccac gagaacaacg gggcactcaa gggcctgagc tactcccgcg ggcgtccatc
     3781 ccccctccgc gctctcgtcg agcagacccc ggttggtcgc caccagcgag aggtccgcgc
     3841 cgtgcaccat gctgtcgtag agctccagca tggtctccag gccatagagg gcagggtcga
     3901 ggaaggagga gccctcccaa cgatcccaca cagccgtcgg agacgttgca ccgagttgat
     3961 ggtagcggac ctggcgctct tgttggccca tcacctccgc cagcgcggcg gttatcgcca
     4021 gcgcgacacc ggtgcggtgc gtgggtgcga gtaatatcct tgccgctgac actggtggca
     4081 cgactccttc tcggacagta tgaaatccta cacccgacgg gccgtgctgc cgaataggga
     4141 gagaatggct ctcatcgctc gcatacggag cactttcgga cgactggacg atctgatcat
     4201 cgcccggtcg atactcactg gttcgctggc gctcgcctca ctcctcttcg cgcagacgaa
     4261 gagcggcggg gatctcgact ggtggatggc gctgccgctc gccctcgtcg ccttggccaa
     4321 cctccctctc ttcgtgctct ccgcgcgcgg ccaccgcgtg ggagtgtcgt gggcggtggt
     4381 ggtggttgac acgtgcctga tcacctacct ggtgcacgtc acctcgggcg cgcagggcgc
     4441 cgtcgccgtc ttctacgtgt ggccgatcat cgccgccaac ctcctgctgg gagccagggc
     4501 cggctacctg acggcggccg tgaccgccgc actctacgtg ggcttggcgg cagccgaggc
     4561 cggcggctgg gagccgcgtg cgcttcttga gggtcgcggt ctctcggtgg cggacggtct
     4621 ggacggcgtg ctcgtgcgcg caagcgcctt cctcttgatc gccctgctct cgggcctagt
     4681 gtcgaacgcc ttgctcgatt caaacgcccg tctctcgcag gcgaaggccg acgccgaacg
     4741 cgatctggac cgaaccaagc tgctcaaccg gcgcttgacg atcttggatg aggtggggcg
     4801 gattttgggc cggatccaag acctcgacat cctgctcccc cgcgctctcg gacgcgtggc
     4861 gggtttcctg ggggtcgagg ccggcatgat cgtgttgttc ggcaaagacc ggagcgacat
     4921 ccagatcgcc gcacgccagt ctctgagcga ggcgaccgcg cgcgacctgc tcgcgggaga
     4981 tctgcccgtg tcgctcgacg ccaacagggg ggcggtgctc ggcagcgcgg acggccgttc
     5041 cgcacgagcc ctacggacgc tccaaccact cggctacgcc gacttcctgg ccgcgccgct
     5101 gcgcctcggg ccggaatacg tgggcaacct ctatctcttc accacggcgc cgaacccgtt
     5161 ccgccgagga gacgtcgcct tgatggactc tctctgctcc cagctcgcca tcgccgtcaa
     5221 gaacgtggcc ttcaccaggg agctcaagga cgcgaacgac gagctcgtga acctggatca
     5281 actgaaaagc gacttcctag ccacgatgag ccacgaactg cgcacgcctc tcacctccat
     5341 catcggctac agcgacatgc ttctctcggg gatgaccggc gaggtcaccg acaaacagcg
     5401 tgacttcctc aggagcatcc tcaacagcgg cgaggcgctg ctcgatctga tcaacgacat
     5461 cctggacctc accaagatag aggccggcaa gctcgagctc agcctggaac cggtcaacct
     5521 gagaactgcg ctcgcatccg tgctctcggt ggtgaagccc agagcgaagg agaagaatct
     5581 ccagatatcc accttcctac ccaacgatct gccgcctctc tgggccgact cgggcaagct
     5641 gaaccaggtg ctcctcaacc tgctcaccaa cgcgatcgag tacacgcctg agacgggccg
     5701 tatcggcatc gaggccagac ccacccccga cggtctggtg gagattcggg tgaacgacac
     5761 gggtgtaggc atcgagcccg cggatcttgc gcgcatcttc gaacgtttca cccaggtgga
     5821 caacacgtcc acccgcagtc agggggggac cggcttgggt ctcgcgatca ctcgagacct
     5881 gatcgaactc caccaaggta cgataacagt gaaatctcaa ccagggcggg ggtcccagtt
     5941 catcttcacg atccctcagg cgggcgcaca gaaggagccc cgaagcaggg ccagactcgc
     6001 ataggaggca ggctatggct cgtatcgtcg ttgcggacga cgacccggat attctgaaca
     6061 tcgtcgccat gtcgctcgaa gccatgggac acgaggtgca ccgagcgacg aacggccgtg
     6121 aagcggtcgc tctgacgaag gcgaccgcgc cggacctggt ggtcatggac ctgatgatgc
     6181 cggagatgaa cggatacgag gccaccgagg ccttgaaggc cgacgccgag acggcgtcgg
     6241 tgccggtcct ggcactcacg gccaaggcca tgcgcggcga cgaagagagg gggcgtcaag
     6301 cgggtgtgga cgcctacgtc accaagccgt tccgcatctc gcaggtggtc gaagtggtgg
     6361 atcggctgct ctagcccggc gcgggcgcgc cgcctcgaac cccgccgcgc gcaccatgtc
     6421 gcccgcgacc acccacccaa gcccgcggtg cgccgaatcg gcgcgcgggg tcgggggctg
     6481 tgccgcgggg gcgcgggcgc ggcgacttca ggccgccggc gcgccgcgac ccgcgggccc
     6541 cggttagcgg ttccggctga tgaggagcag tcgcgccagt tgcgcgacag ccatgagtgc
     6601 cgccgcgacg taggtgaggc ccgccgcgct cagcaccttg cgcgctccgt ccacctcggt
     6661 ggagaccaga aggccccgag aactgagcag ggccagcgcc cgccgggaag cgttgaactc
     6721 caccggcaga gtgacaacgg tgaacagcac cgccgctgag aacagcagga tccccgcatt
     6781 catgagcagc gggttccgcc cgatgaacaa acccacgatg aagaggatca tccccaggtt
     6841 gctgcccaga gaggcggcgg gcacgaggct cgcgcggaca cgcatgggcg cgtagccctc
     6901 ggcatcctgc agggcgtgac ccacttcatg cgccgcgacg ccgagcgcgg cgagagaatc
     6961 ccggcccgcc acgtcggggc tcagccgaag caccttcgtc cgagggtcgt agtgatcgga
     7021 gagtcgtccc tgcaccggtt cgatcggcac gtgcgaaagg ccgcccgcgt cgagtaggag
     7081 acgggcgccg gccgcgccgg tgaggccgcg gcctgaagga accttgctgt agtgggcgaa
     7141 ggtggtcttg accttccatt gcgcatagag ggacaaggca agcgccggga gtagtaatat
     7201 gaaggtccaa tcgaagaagg gaaacacgac gcgctcctat gtggttcgag gatttcggtt
     7261 atgtcgggtt acgccgcgag acggacttgc cgatgtgtag gcccttcggc ttatggactg
     7321 tcggctggcc catgtgatga tagcagagac gccggatgct agtacccccg ccccctatag
     7381 gaagcaggtg aacccgatgc cggtccgccc aggcaacggc accggccggc tcggcgacat
     7441 cctcgtcgag aggaagctgg taacccagga caacctcgag cgcgctctca gttaccagaa
     7501 ggagagcgga tcgagacttg gcgaagcgct gatcaaatta ggctacatca gcgatataca
     7561 cctggcggag gcgttggcgg cacagaagaa gttgccgatc atcgcgctgg gtgaggtctt
     7621 cccgaatccg cgcgccgcct ctctgctcac agagaagttc atccgtgccc gtcgggtgat
     7681 gcccgtcgac ttcaaccagg acgcgctgat cctggccatg gtcgacccgc tcgacgtggt
     7741 gacgctcgac gacgtgcgcg tgatcaccgg cctcgaagta gagccggtgg tagccaccgc
     7801 cagcggtttc gccgacacgc tcgagtacgt cttctcgggc aggggcgtgc tcgatgcttc
     7861 cgagagggac gaaccgcgcg actcccgcca ggtcgctgag gagcggcgca acgc
//
LOCUS       DASRMJ010000027         6881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_219351, whole genome shotgun sequence.
ACCESSION   DASRMJ010000027 DASRMJ010000000
VERSION     DASRMJ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219351"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VFD74_00665"
     CDS             <1..1227
                     /locus_tag="VFD74_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK48099.1"
                     /translation="GIGETVPRIAPDIAALDREYVLPTYVRLPLEVVSGLGGEVVGRD
                     GRRYLDFVTGLSVSNFGHCHPRVVAAIREQAGRLIHCSNLYTTEPQARLAGRLSALAN
                     GGRVFFSNSGAEANELAVKIARKRARSLGGGVIVTVEGSFHGRTMATLSATGQPLKQK
                     DFGPPLDGFLHVPFGDVAALETSFREHRVAAFMVEPVLGEGGVRIHPDGYLEAAGLLC
                     AQHDALLMVDEVQTGLGRCGAFFAYQRFGLNPDVVTVAKTLAGGLPMGGTVVAGRAEG
                     VIVAGDHGSTFGGGPVVAAAALAVLDLVEGDGLFAAVEDAGARLEGWLRTLEKAGAVR
                     EVRRLGLMAAADLTDKSAKQVVLDALDAGILLNATSDETLRFLPPLTVSPEEIDRVGD
                     FLVTLLVPTRIPGGAA"
     gene            1224..2144
                     /gene="argF"
                     /locus_tag="VFD74_00670"
     CDS             1224..2144
                     /gene="argF"
                     /locus_tag="VFD74_00670"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZK48100.1"
                     /translation="MTPRHFVKVTDLTSEEIRWVLEEAKRMKSHPAGDLLEGKTVAMI
                     FSKPSTRTRVSFSVGVHQLGGMPLFLGEHELQMRTSETIEDTARVLSRYVDAIVIRTF
                     AQQDVEDLAANSTVPVINALTDDHHPCQALADIFTFEEVFPDARGRTITYLGDGNNVA
                     ASLSAAAALTGIDVCLATPEGYDLPAAVWDEVQRVAAAHGTRVWLERDPAAAAANASA
                     LYTDVWISMGQSADAEKVAALTPYRIDDDLLAKARPEAVVMHCLPAHRGMEITDSVMD
                     GPRSVVFDQAENRLHAQKALLSFLLRGIWG"
     gene            2141..2596
                     /locus_tag="VFD74_00675"
     CDS             2141..2596
                     /locus_tag="VFD74_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HZK48101.1"
                     /translation="MNKRQRQNLTRDVIRRARVGTQRELVQALLGLGCDVTQATVSRD
                     LRELGVERTRDSLGRFRYSLPEHEERRDPKAACARMLEEFGTAVLAAQNIVLVKSEVG
                     TAPGMGRVIDELQHELILGSVAGDDTVMIVTADSAAAQAVADYLTQLGG"
     gene            2599..3798
                     /locus_tag="VFD74_00680"
     CDS             2599..3798
                     /locus_tag="VFD74_00680"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZK48102.1"
                     /translation="MPTDLCVLAYSGGLDTSALIPYLRETRDMDVVAALVDVGRAKDL
                     ESLRQRALDAGAVDAVVIDAKDEFVRDFVLPALAANALYEEKYPLVSALSRPLISKKM
                     VQVAQERGATFIAHGCTAKGNDQVRFDVSIRCLDPRITVLGPAREWNMTRPQLLDYVA
                     ARGIEIPLTKKNPFSIDENVWGRTIECGELEDPWVAPPEEAFTVTVDPRRAPDEGVET
                     IVTFDKGVPVALDGVAMEPSALIDSIDALGGAHGFGRIDMMENRLVGIKSREIYETPG
                     ALALIMAHKEVEDLVLTRDLLHFKRGVEQRLADMIYDGQWFSPLADSLRAFVSHTQAR
                     VTGDARLLFYKGSCSVRGRRSPYSLYVEELATYGEGDRFSHESAKGFIELWGLPLEVW
                     ARREKSA"
     gene            3795..5273
                     /gene="argH"
                     /locus_tag="VFD74_00685"
     CDS             3795..5273
                     /gene="argH"
                     /locus_tag="VFD74_00685"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZK48103.1"
                     /translation="MSHRDEDTASGAGAAGHRPSDGTGRREKAWGGRFAESQAPLFER
                     MNASLPFDYVLGAIDVRGSAAHVRMLGKIGVLTDEETGILLEGLATVSAEIEAGDLIW
                     DLGDEDVHMAVERRLTELVGPVGGKVHTGRSRNDQVALDLQLYLRQAVAGHLTGILDL
                     RDALLGRAESWQDVVVPGYTHLQRAQPVSLAHHVLAYVAMLERDTTRFLNWHESSWMP
                     LGAGALAGVNYALDREMVARELGFARVAPNAMDAVSSRDSAFEYVAAATACALNLSRF
                     AEEIVLWSSQEFGFFSLPDSWSSGSSIMPQKRNPDAAELVRGKAAGFLGRLTSLGALS
                     KGLPLAYNKDLQEDKLYVFATREELDLCLQAMAAMVRVVSVDRERARLAAEGGYSQAT
                     DVADLLVAKGLPFREAHHVVGRLVAFAHAAGKSLAEISPQELAGLSPLFDQEYYDAVD
                     LERVVGAKVSPGGTSPQRVAEQLTEARSRVDDARIRVREVRS"
     gene            5356..6312
                     /locus_tag="VFD74_00690"
     CDS             5356..6312
                     /locus_tag="VFD74_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HZK48104.1"
                     /translation="MRLGDLYNVAVEAGKDADPRGSKGIERALSEAKKAFDELPEPRR
                     WEFDRESLTNPFSDTRILGGDPDTDVRRMLVGIDISVGEVLLADRLREKGEALDLVLA
                     HHPEGRALADLSHVMGVQADIWRTFGVPIGFGDGMMAGRRAEVDRRFHASNIDQAVSA
                     ARILGIPLMCCHTPADNNVNSFLQAKCDEFDEDATVGDIVDMLKTIPEYREAVQSGVG
                     PVIFQGAADFRAGKIMVDMTGGTSGPEEAIDRLASAGVGTILGMHMGEDHRKRAEAAH
                     LNVVIAGHHASDSLGMNLVIDRMAAAGVEVVGCSGFTRVSRV"
     gene            complement(6354..>6881)
                     /locus_tag="VFD74_00695"
     CDS             complement(6354..>6881)
                     /locus_tag="VFD74_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZK48105.1"
                     /translation="PQGLVYAQDPLAGSMLLQGSTVRLAVATKGAPQGTVPNVVGLQV
                     AKVTRSLRAAGYLVSESQVVSDAPVGTVVDQTPAGGAAAAAGARVDIVVSTGPPTGST
                     DTTAPSVEVPSLVGERLARALQLLGQRGLAGEVTGATTTDDPDKQGTIASQSPETGTQ
                     AAMGTVVSLTVFGPP"
BASE COUNT         1127 a         2209 c         2388 g         1157 t
ORIGIN      
        1 ggcattgggg agaccgtgcc gcgcatcgcc ccggatatcg cggccctgga tcgcgaatac
       61 gtcctgccga cctacgtgcg cctacctctg gaggtcgtga gcggcctggg aggcgaggtg
      121 gtcggacgcg atggtcgccg ctaccttgat ttcgtcaccg gcttgtcggt gagcaacttc
      181 ggacactgcc atccacgcgt ggtcgcggcg atccgggaac aggccggccg gctcattcac
      241 tgctcgaacc tctacacgac ggagccccag gcccgactgg ccggccgcct ctccgcactg
      301 gccaacggag ggcgggtctt cttctcgaac agcggggctg aggccaacga actcgcggtg
      361 aagatcgcgc gcaagcgcgc ccgctcgctc ggcggaggcg tgatcgtaac ggtggagggc
      421 tccttccacg ggcggaccat ggctaccctg tcggctacgg gccagcctct gaaacagaag
      481 gacttcggcc ctcccttgga tgggttcctg cacgtgccct tcggcgacgt cgcggccctc
      541 gagacgtcct tccgcgaaca ccgcgtggcg gcgttcatgg tggagcccgt gctgggcgaa
      601 gggggcgttc gcatccatcc cgacggatac ctggaagcgg ccggtctcct gtgcgcccag
      661 cacgacgcac tgcttatggt ggacgaggtc cagaccgggc tgggacgctg cggcgccttc
      721 ttcgcctacc agcgctttgg gctgaacccg gatgtggtca cggtagccaa gactctggcg
      781 ggcggcctgc cgatgggcgg caccgtcgtg gccggacggg cggagggggt catcgtcgct
      841 ggggaccacg ggtccacctt cggcggcggc ccggtggtgg ccgcggccgc cctcgcggtg
      901 ctcgacttgg tggagggtga cggtctgttc gcagccgtgg aggacgctgg ggcccgcttg
      961 gaggggtggt tgcgcacgtt ggaaaaggcc ggggccgtcc gagaagtgcg tcggctggga
     1021 ttgatggcgg cggcggatct gaccgataag tccgccaagc aggtggtgct ggacgcgctc
     1081 gatgccggca tccttctgaa cgcgacgtcc gacgagacgc tccgcttcct gcctccgctc
     1141 acggtgagtc ccgaggagat cgaccgcgtg ggcgacttcc tggtgaccct actcgtcccc
     1201 acccgcattc caggaggcgc cgcatgactc cccgccactt cgtcaaggtg accgatctta
     1261 cgtcggagga gatccgctgg gtgttggaag aggcgaagcg aatgaagagc catccggccg
     1321 gcgacctcct ggaggggaag acggtggcca tgatcttcag caagccctcc acccgcacga
     1381 gggtctcgtt cagcgtcgga gtgcaccagt tgggcggcat gccactcttc ctgggagagc
     1441 acgagctcca gatgcgcact tcggagacga tcgaggacac ggcgcgggtg ctttcgcgct
     1501 acgtcgacgc catcgtcatc cgtacgttcg cccagcagga tgtcgaggat ctcgccgcca
     1561 actccaccgt gccggtgatc aacgccctca ccgacgacca tcacccgtgt caggcactgg
     1621 ccgatatctt cacgttcgaa gaggtgttcc cggatgcccg gggtcgcacg atcacctacc
     1681 tgggcgacgg caacaacgtc gcggcgtcgc tgtccgctgc cgccgctctc actggcatcg
     1741 acgtctgcct ggccacgccc gaagggtatg accttccagc cgccgtctgg gacgaggtgc
     1801 aaagggtggc ggccgcccac ggcacacggg tttggttaga gagggatccg gcggcggccg
     1861 ccgcgaacgc gtccgctctc tacaccgacg tgtggatcag catgggccag agcgccgacg
     1921 ccgagaaggt ggcggcgctc actccctacc gcatcgacga cgacctgctc gcgaaggccc
     1981 ggcccgaggc ggtggtcatg cactgtctgc cggcacatcg gggcatggag atcacggact
     2041 ccgtcatgga cggtccgcgg tccgtggttt tcgatcaggc cgagaaccgg ctgcacgctc
     2101 agaaggccct gctctcattc ttactcaggg gcatctgggg gtgaacaagc ggcaacgcca
     2161 aaatctcacg agagatgtga tccggcgcgc ccgggtcggc actcagcggg agttggtgca
     2221 ggccctcctg ggcctcggat gcgacgtgac gcaggccacc gttagtcgcg acctccgcga
     2281 gttgggtgtg gagcgcacgc gcgactcctt ggggcggttc cgctactctc tccccgagca
     2341 cgaagagcgt cgcgacccca aggccgcgtg cgcacgcatg ctcgaggagt tcggcacggc
     2401 cgtccttgcc gcccagaaca tcgtgttggt gaagtccgag gttgggaccg cccctggaat
     2461 ggggcgcgta atcgacgaac tccagcacga actcatcctc ggctccgtgg ccggggacga
     2521 caccgtcatg atcgttactg cagacagcgc cgcggctcag gccgtggccg actacctcac
     2581 ccaactagga ggctgacgat gccaaccgac ctctgcgtgc tcgcctactc aggcggcctc
     2641 gacacctcgg cgctgatccc ctacttgcgc gagacgcgcg atatggacgt agtcgccgct
     2701 ctggtggacg tgggccgggc gaaggatctc gagtcgctgc ggcagcgggc tctcgacgcg
     2761 ggggccgtgg acgcggtcgt catcgacgcc aaagacgagt tcgtccgcga cttcgtgctc
     2821 cccgctctgg cggccaacgc cctctacgaa gagaagtatc ccctggtctc cgccctctcg
     2881 cggcctctca tctccaagaa gatggtgcag gtggcccagg agcggggcgc gactttcatc
     2941 gcgcacgggt gcacggccaa agggaacgat caggtgcgct tcgacgtttc catcaggtgc
     3001 ctcgatccgc gcataacggt cttggggccg gcacgcgagt ggaacatgac ccggcctcag
     3061 ttgctggact acgtagccgc ccgcggcatc gagatccccc tgacgaagaa gaaccccttc
     3121 tccatcgacg agaacgtgtg ggggcggacg atcgagtgcg gagaactcga ggatccctgg
     3181 gtagccccgc cggaggaggc gttcacggtg acggtcgatc ccagacgtgc gccggacgag
     3241 ggcgtggaga ccatcgtgac cttcgacaag ggtgtcccgg tggccttgga cggagtcgcg
     3301 atggagccgt ccgcgctcat cgactcgatc gacgcgctgg gtggagctca cggtttcggc
     3361 cgtatcgaca tgatggagaa ccggctggtg ggcatcaaga gtcgtgagat ctacgagact
     3421 ccgggcgcct tggcgctgat aatggcacac aaggaggtcg aggatctcgt tctcacccgc
     3481 gacctcctcc atttcaagcg cggtgtggag cagaggctcg ccgacatgat ctacgacggt
     3541 cagtggttca gccccctggc cgactcgctg cgggcgttcg tctctcacac gcaggcccgc
     3601 gtcacgggcg atgcgcgcct cctcttctac aagggttcct gcagcgtgcg cggtcggcgt
     3661 tccccctatt cgctctacgt cgaggagttg gccacctacg gtgaaggtga tcgcttcagc
     3721 cacgagtcgg cgaagggttt catcgaactc tggggactcc cgctggaagt gtgggctcgt
     3781 agggagaagt cggcttgagc caccgcgacg aggatacggc gtcgggcgcg ggcgcagctg
     3841 gccaccggcc ttctgacggg acggggcggc gggagaaggc ctggggcggt cggttcgcgg
     3901 agtcgcaggc gccgctgttc gaacgcatga acgcctccct gccgttcgac tacgtgctcg
     3961 gcgccatcga cgtccggggc tcggcggcgc acgtccgcat gctcggcaag atcggggtgc
     4021 tcaccgacga ggagacgggc atcttgctgg aaggcctggc cacggtctcc gcggagatcg
     4081 aggccggcga ccttatctgg gaccttggcg acgaagacgt gcacatggcc gtggaacgca
     4141 ggctcaccga actcgtcggc cctgtgggcg gcaaagtcca caccgggcgc agccggaacg
     4201 accaggtggc tctcgacctc cagctctacc tgcgtcaggc cgtggccggc cacctcaccg
     4261 gcatcttgga cctgcgggac gccctgctcg gcagagccga aagctggcag gatgttgtcg
     4321 tcccggggta cacgcacctg cagcgggccc aacctgtgag tctcgcgcac cacgtgttgg
     4381 cctacgtggc catgcttgag cgggatacga cccgcttcct caactggcac gagtcgtcgt
     4441 ggatgcccct cggcgcgggg gcgctggccg gtgtcaacta cgcgctcgac cgtgagatgg
     4501 tggcgcgcga actaggcttc gcgcgcgtcg ctccgaacgc gatggatgcc gtctcctcgc
     4561 gcgactcggc cttcgagtac gtggccgcgg cgaccgcctg tgcgctgaac ctctctcggt
     4621 tcgccgagga gatcgtccta tggagctcgc aggagttcgg gttcttctct ctgcccgact
     4681 cgtggagttc gggatccagc atcatgccgc agaagaggaa ccccgatgcc gccgagctcg
     4741 ttcggggcaa ggccgccggg ttcctgggtc gactaacgtc tctaggcgcc ctcagcaaag
     4801 ggctgccgct ggcctacaac aaggacctcc aggaggacaa gctctacgtc ttcgccacca
     4861 gggaagagct cgacctctgc ctgcaggcga tggccgccat ggtcagggtg gtgtctgtag
     4921 atcgggaacg ggcgcgtctc gcggctgagg gcggctactc acaggcgacc gacgtggccg
     4981 atctcctcgt ggcaaagggt cttcccttcc gggaggcaca ccacgtcgtg ggccgattgg
     5041 tcgcgttcgc gcacgccgcg ggcaagtcgc tggcggagat ctcgccccag gagctggccg
     5101 ggttgagccc gctcttcgac caggaatact acgacgcggt cgatctcgaa cgggtggtcg
     5161 gggcgaaggt gtcgccggga gggacctcgc cgcagcgcgt ggcggagcaa ctgaccgaag
     5221 ctcgttcgcg ggtcgacgac gcccgcattc gagtgcggga ggtgcgcagc tgattgatct
     5281 tcggccggcg gtcgaagcga gggcgcgcga acgacaagcg agactcttac cagagagcga
     5341 gaagggagca gacggatgag gcttggggat ctgtacaacg ttgccgtcga ggcgggcaag
     5401 gatgccgatc cgcgcggcag caaagggatc gagcgagctc tatccgaggc gaagaaggct
     5461 ttcgacgagc ttcccgagcc gcggcgctgg gagttcgacc gggaatcgct cacgaaccca
     5521 ttcagtgaca cccgcatcct gggcggcgat ccggacacgg acgtgcgcag gatgttggtg
     5581 gggatcgata tctccgtggg cgaagttctg ttggcggatc gtttgcgtga gaagggcgag
     5641 gcgctggatc tcgtcctggc gcaccatccg gagggcaggg cgctcgccga cctgagccac
     5701 gtcatgggcg tacaggccga catctggcgc accttcggcg tgccgatcgg gttcggcgac
     5761 ggcatgatgg ccggtcgcag agccgaagtg gaccgtcgct tccatgcgag caatatcgat
     5821 caagccgtct cggcggcgcg gatactcggt atcccattga tgtgctgtca caccccggcc
     5881 gacaacaacg tgaactcctt cttgcaggcg aagtgcgacg agttcgatga ggacgccacc
     5941 gtcggggaca tcgtcgacat gctcaagacg atcccggagt acagggaggc cgtgcagtcg
     6001 ggagtcggtc ctgtgatctt ccaaggtgca gcggactttc gagccggcaa gatcatggtc
     6061 gacatgaccg ggggcaccag tggtcctgag gaggccatcg accgtctggc gagcgcgggg
     6121 gtaggcacca tcctggggat gcacatgggg gaagaccacc ggaagagagc cgaagcggcc
     6181 catctgaacg tggtcatcgc gggccaccat gcctcggatt ctctcggtat gaacctcgtc
     6241 atcgatcgga tggcggccgc gggcgtagag gtagtgggtt gctcaggctt cactcgcgtg
     6301 agccgcgtat aggggctcac gacgagaagg cgggcccccc ggacagacgc ggactacggg
     6361 gggccgaaga ccgtcaacga caccacggtc cccatcgccg cctgggtccc tgtttcgggg
     6421 gattgggagg cgatcgtgcc ttgcttgtcc gggtcgtccg tggtggtggc tcccgtgacc
     6481 tcacccgcga ggcctctctg gccgagcagc tgcagcgcgc gggcgaggcg ctccccgacg
     6541 agggatggca cctccaccga cggcgccgtg gtgtcggtgc tgccggtggg aggacctgta
     6601 cttacaacga tgtctacccg tgcccccgca gccgccgctg cgcccccggc gggggtctgg
     6661 tccaccacgg tgcccacggg cgcatccgag accacctggc tctctgacac cagatagccc
     6721 gcggcccgca gactccgggt gaccttcgcg acttggaggc ccacgacgtt gggcacggtg
     6781 ccctgcggcg cgccctttgt ggctacggcg agtcgcacgg tactgccctg tagaagcatg
     6841 gaccccgcca acgggtcttg ggcgtacaca aggccctgcg g
//
LOCUS       DASRMJ010000028        14786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_437015, whole genome shotgun sequence.
ACCESSION   DASRMJ010000028 DASRMJ010000000
VERSION     DASRMJ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 14786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14786
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437015"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /gene="pheT"
                     /locus_tag="VFD74_00700"
     CDS             complement(<1..1760)
                     /gene="pheT"
                     /locus_tag="VFD74_00700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZK48106.1"
                     /translation="MKAPLSWLREHVTIDLPVDELASRLALTGTEVERVALVGVPARD
                     DVIERFVVGKVLTREKHPNADKLSVCTVDVGEDKPRTIVCGAHNVAAGQTVAICLPGA
                     VLANGMLIGEAKLRGVASSGMIMSEAELGFEQKSEGIVALPDDWEAGEALADRLPISE
                     YVLEVEVTPNRPDCLSIRGLAREIGAVTEAAFCEETDFTFPVGERAVEDDVTVEVWDP
                     DLCPRYAARVVRGITVQPSPPWLKARIAHAGMRPVNNVVDITNYVMWTVGQPLHAFDL
                     SKIEGGGVIARRARDGETIVTLDGSERVLTSEMLVIADKVKPSVIAGIMGSLDSEITN
                     DTTDLLLEAANFHGPSIMRTSGALGLRSESSTRFEKGLDRNLVGLALDMACSMLTDMC
                     GGSVSKGTIDVLAGEKKPLVLSLRPHRVVEILGAEVLTSEIESILGTLGCDVDEDPGD
                     DERLLVTVPTFRSDLEREIDLVEEVARIHGLDRLPSTLPPRTEGRGGLTKTQTAARRV
                     ADALVGAGLDEVVTYSFVEPGWFDRLGLTPADPRHETIALANPLSQDQSVMRTMLLPG
                     LLATAAKNVAVREDRVHVFE"
     gene            complement(1819..2799)
                     /gene="pheS"
                     /locus_tag="VFD74_00705"
     CDS             complement(1819..2799)
                     /gene="pheS"
                     /locus_tag="VFD74_00705"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZK48107.1"
                     /translation="MAAVESAVSLAALEEARVRLLGRRAELTQLLRSIPQLAVEERPA
                     VGRFGNLIKRDLEKALEERQVSLEAEELVGSLERDAVDVSLPGVSMPLGHLHLITSTL
                     REIEDIFVSMGYEVAEGREIETDYYNFEALNHPADHPARSLHDTFFVRDDILLRTHTS
                     PVQVRTMERRRPPVYIIAPGKAYRRDSDATHTPMFHQVEGLVVDRDITLADLKGTLEV
                     FARAMFGADRRIRLRPHFFPFTEPSVEVDVSCMICDGVGCRLCKRSGWLEILGAGMVD
                     PNVFGFVGYDPEEFTGFAFGMGIERIAMLKHGVTDLRLFYENDLRFVRQF"
     gene            complement(2891..3736)
                     /locus_tag="VFD74_00710"
     CDS             complement(2891..3736)
                     /locus_tag="VFD74_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZK48108.1"
                     /translation="MIREIAGRHNDSIKLAQKLQKKRHRRDRALFVCEGFDLLLAAVR
                     AGELPVEVLVRKDLLDRLPAELLSEAEEDELDIGVCEEEVMAQASALGGAADVVCVFE
                     AVEWSLGDLAIDAGVTVYVAGVGDPGNVGTIVRSAVAFGAVGVICSPGTADPYGPKAL
                     RAGMGAQFLTRVVVEVTPGDIAAKLEAEGRRGGLVPQVVVADLQGDVEARDLSMEGPA
                     ILVLGAERGGLPEFSTPTRRVVIPQAGFDSLNVAMAGTILLYELTQERLTGGRLGKAG
                     EGLLA"
     gene            complement(3852..5177)
                     /locus_tag="VFD74_00715"
     CDS             complement(3852..5177)
                     /locus_tag="VFD74_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017692454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HZK48109.1"
                     /translation="MHMRLSPPRVVLLSFAAAILIGSAVLSLPGMTAAPGSLAYIDAL
                     FTAASAVCVTGLITVDTATVFTPLGKAVIAILIQLGGLGIMTAATMLFLLARRRIGLQ
                     GRLVIQETLGHLRLAGVVRLVLAILLMTLVFEGVGTLILAGDFLLHHGYSPLRALGYG
                     AFHSISAFNNAGFALFTNSLEGFRDDWVVTLTVACLIIGGGLGFPVILDLARPRKGRI
                     YALQTKMVILVTAGLLSVGTLAFLALEWTSPELAGLGAPGRVLVAFFQAVTPRTAGFN
                     TVPIAGLAPGTIFLFIILMFIGASPESTGGGIKTTTFGIIAASMRATFRGAGDTEFAS
                     RRIGQAQVTKALTLVVASAVFILVMTLLLLDTGQDIPLDTGLFEVVSAFGTVGLSLGL
                     TSKLTMAGKVVIILTMYAGRVGTLTVGAALIARRRPAPRRLPEEALPTG"
     gene            complement(5218..5577)
                     /gene="rplT"
                     /locus_tag="VFD74_00720"
     CDS             complement(5218..5577)
                     /gene="rplT"
                     /locus_tag="VFD74_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018120129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZK48110.1"
                     /translation="MARIKRSVNAKKKRRKALEEAKGYRGLKSVNYRQAKEQLLKSYT
                     YAYRDRKARKSEFRKLWIQRINAGAREHGLSYNQFIHGLKEANIELNRKMLADLAVRE
                     PQVFAEIAGKAKAALTA"
     gene            complement(5590..5781)
                     /gene="rpmI"
                     /locus_tag="VFD74_00725"
     CDS             complement(5590..5781)
                     /gene="rpmI"
                     /locus_tag="VFD74_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZK48111.1"
                     /translation="MKKKTHKGMKKRIKRTATGKLVHRRAFSSHILTKKPSKRKRKFR
                     KDVAVDSTNLGHCRALLGI"
     gene            complement(5906..6418)
                     /gene="infC"
                     /locus_tag="VFD74_00730"
     CDS             complement(5906..6418)
                     /gene="infC"
                     /locus_tag="VFD74_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZK48112.1"
                     /translation="MAETTRINDRIRAESVRLIGPAGEQVGIVSLREAAEYADRLNLD
                     LVEVAPMASPPVCKVMDYGKYRYEQEQKAKEARKRQTTISIKEIKLRPKIDDHDFATK
                     KRHVERFLAKGDKVKLTIMFRGRELVHPHLGERLLRRMAEELQELGDVESQPNLDGRN
                     MIMMLAPKKH"
     gene            complement(6703..8610)
                     /gene="thrS"
                     /locus_tag="VFD74_00735"
     CDS             complement(6703..8610)
                     /gene="thrS"
                     /locus_tag="VFD74_00735"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZK48113.1"
                     /translation="MDVILPDGTKLSVPEGATALTVAETIGPRLAKAAVAATVGGRLV
                     DLATPVHEGDQVSIVTPASEEGLQVLRHSSAHVLAEAATRVFPGTKVAIGPAIKDGFY
                     YDFEFPEPVTEDDLARFQAEMERSIKEARPFVREEVSREQALELFAGEPYKAELIRDL
                     PQGETISLYRHGDFVDLCRGPHLPDTGRIPAVRALSIAGAYWRGDSANTMLTRVYGTA
                     FWSKKDLDVHLEKLEMARLRDHRKLGRELDLFSFHDEGPGFPFFHAKGMRVINALLDY
                     WRAEHRAAGYEEIKTPIILDRALWEQSGHWENYKDNMYFTRIDDADYAVKPMNCPGGI
                     LIYRAEQHSYREFPLRMAELGLVHRHELSGVLHGLFRVRTFTQDDAHIYCLPEQIEDE
                     VVGVIELTRKIYRTFGFDRVHLELSTRPEKSIGSAEMWETAEKALAGALDRAGLEYTL
                     NPGDGAFYGPKIDFHVEDVMGRTWQCATCQLDFAMPERFDLEYVGDDNQRHRPVMIHR
                     TVLGSIERFLGILIEHYGGAFPAWLAPVQAVVIPVADRHNEYARQTAAALAAVGLRVE
                     VDDRTESMGRKIRDNEVLKVPFMVVVGDRESQGDTVSLRSHADGDLGTRARSLAIDEI
                     AAASRMPVIVA"
     gene            complement(8809..8880)
                     /locus_tag="VFD74_00740"
     tRNA            complement(8809..8880)
                     /locus_tag="VFD74_00740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8846..8848),aa:Val,seq:gac)
     gene            complement(8940..10190)
                     /locus_tag="VFD74_00745"
     CDS             complement(8940..10190)
                     /locus_tag="VFD74_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK48114.1"
                     /translation="MIVSTLAALLLLGVGVSAASALPFPDVEPTDWFYEAVDVLSDAG
                     VIMGLPGGTFGPYQTVTRAQFAAMLSRALATPEAVDEPFDDVSDTDWFQAPVASLYEA
                     GLLMGTTRTTFSPSLGIPRQQVATLMIRALVYRFQTEPQEGVEIALDSAQVDGWLAGF
                     SDRYDIYAIHRAEVADAVRLGVVKGTLDGRFMPLGTLTRAQAAVLLYRALYQPLVPLT
                     TQPAPVPAIPQVTLQQGSTGAYVALLEQRLAALKYAVGVADAKFDEALRDAVRAFQKV
                     EGLERTGTVGPSVWKRLDTAVTPSPRNWAAGERVEIALTRQVLFLISGNRVTRILAVS
                     TGASSTPTPAGSFRILRKIDAWRESSLGLLYKPSYFYGGIAIHGSYSIPTYPASHGCV
                     RIPYWATDAIYPLLPVGMPVDVYR"
     gene            complement(10299..10370)
                     /locus_tag="VFD74_00750"
     tRNA            complement(10299..10370)
                     /locus_tag="VFD74_00750"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10335..10337),aa:Cys,seq:gca)
     gene            complement(10431..10507)
                     /locus_tag="VFD74_00755"
     tRNA            complement(10431..10507)
                     /locus_tag="VFD74_00755"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10471..10473),aa:Gly,seq:gcc)
     gene            complement(10663..12282)
                     /locus_tag="VFD74_00760"
     CDS             complement(10663..12282)
                     /locus_tag="VFD74_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZK48115.1"
                     /translation="MLVEDLERMIELAAPRALAESWDNVGLLVGRRGTELRRVLVALD
                     LTEEVVKEAVVEGFQAIITHHPLLFAPITRVTDADRVGVLITQLVAADVALLAAHTNL
                     DGAPGGLCDLVAREMGLAHILPLARTPSRVKKLVGFIPEGEVERVSRAVFEAGGGRIG
                     DYQGCSFENRGEGTFVAGPSTHPTVGRAGESVRVAEVRWETIVPENRVVAAVQAFICA
                     HPYEEPAFDVYPVENVSLTGGQGRVGSTRSEVSLVALAETAAEVFGLPEVSFAGDIEH
                     MVDRVAVVTGSGSSFIAEAARSADVLLTGDLKYHDAEKAADAGLALVAVPHGHLETWA
                     LRRWCEGFGAAVAEHDVEVVFSQAGRSPWHAAKSVSHAGEANGVVRLFDLEEAGGERV
                     GGPDSDDAPNGEPTRTFTLRVDGASRGNPGPGAIGVVLEDEQGEIVEELGARIGVATN
                     NQAEYQALITGLETALDRSIGRLRILSDSELLVRQLRGEYRVKSEQLKELYLQARALV
                     QRFERVEIVHVAREENSRPDELANQALDGGR"
     gene            complement(12413..14125)
                     /gene="pknB"
                     /locus_tag="VFD74_00765"
     CDS             complement(12413..14125)
                     /gene="pknB"
                     /locus_tag="VFD74_00765"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HZK48116.1"
                     /translation="MIGRVFEDRYEIARKLGSGGMADVYLANDRLLGRQVALKILSTR
                     YAHDEQFVERFRREASSAAGLNHPNIVQIYDRGEAEGTYYIAMEYLEGRSLKEIILTC
                     APLSSDLLISISVQILEALRSAHRRDIIHRDIKPQNIIIDNDGRAKVTDFGIARAGSA
                     STMTEAGSILGTAQYLSPEQAQGQPVEAASDLYSLGVVMYEMATGRPPFDGDNPVSIA
                     TQHVHEHPIPPRNIAPDAPEDLEAVILRSLGKKPTERYLTAQAMLDDLRKVQDGQAIS
                     APAVFREEATRVMSPAAAAFVAQPTQVRPKVSDALDRAPEPQHDEPPRRRSVWPWILV
                     VVLILALAGGAYAISSNFGSAGTDLVVVPGVMGLSEAEARQKVEAAGFVFKNAGDQPS
                     ADVKAGDVARQDPEESTKLAKGKSVSVWLSSGKGTVKTPDVVGMNQDAAVDLIEGMKL
                     VATVQPVASTLTGGTVVDQNPKKGTDAQPGSTVIISISNAPTMVIVPAVVGLSPEQAT
                     AMLATYSLRARIVLRETRDYPPNLVIGESPTAGVEVEKNSYVDLIVSTVTSPRPHGHP
                     KKEE"
     gene            complement(14441..>14786)
                     /locus_tag="VFD74_00770"
     CDS             complement(14441..>14786)
                     /locus_tag="VFD74_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48117.1"
                     /translation="FEQYMEGIQSELTRADVRTKLYRSLFLCMWPTSKRKKLADHAKD
                     YDAIVVLACDSAAETVRDAVSSCGCKVIQGMEVAGITNVKVSFGLPFNVSFKEAKMIP
                     ISQRKNQASAPD"
BASE COUNT         2590 a         4969 c         4586 g         2641 t
ORIGIN      
        1 atctcgaaca cgtgcacccg gtcttcgcga acagccacgt tcttggcggc ggtggcgagc
       61 aggccgggaa ggagcatcgt gcgcatgacc gactggtctt ggctgagtgg gttcgccaag
      121 gcgatggtct cgtggcgagg atctgcgggc gtcaagccca agcgatcgaa ccagcccggt
      181 tccacgaacg agtaggtcac gacctcatcg aggccggcgc ccacgagggc gtcggccacc
      241 cgacgcgccg ccgtctgcgt cttggtgagc ccgccccgac cttcggtccg gggcggcagc
      301 gtcgagggga gccggtccag gccgtggata cgggcgacct cctccacaag gtcgatctcg
      361 cgctcgaggt cagaccggaa ggtgggcacc gtcaccagca ggcgctcgtc gtcgcccgga
      421 tcctcgtcta cgtcgcaccc gagggtcccc aggatgctct cgatctccga cgtgagcacc
      481 tccgccccca ggatctcgac cacgcggtga ggccgaagcg agagcaccag aggcttcttc
      541 tcccccgcca gcacgtcgat cgttcccttg gagacgctgc ccccgcacat atcggtgagc
      601 atggagcacg ccatgtccag cgccaaccca acgaggttgc ggtccaatcc cttctcgaaa
      661 cgagtggacg attcgctccg aagtccgagg gctcccgacg tgcgcatgat cgaagggccg
      721 tggaagttgg ccgcctcgag cagcaagtcg gtggtgtcgt tggtgatctc gctgtccagg
      781 ctgcccatga tgcccgcgat caccgagggt ttgaccttgt cggcgatgac cagcatctct
      841 gacgtgagca cccgttccga tccgtccagc gtcacgatcg tctcgccatc tctggctcga
      901 cgcgcgatga cccctccacc ctcgatcttc gagaggtcga acgcatgcag tggttgtccc
      961 acggtccaca taacgtagtt ggtgatgtcc accacgttgt tgaccggacg catgccggcg
     1021 tgcgcgatac gcgctttcaa ccacggcggc gaaggctgca ccgtgatccc gcggaccacg
     1081 cgggcggcgt acctggggca cagatccgga tcccacactt cgaccgtcac gtcgtcctcg
     1141 acggcgcgct cccctaccgg gaacgtgaag tcggtctctt cgcagaaggc ggcctcggtg
     1201 accgcgccga tctcgcgcgc aagcccacgg atcgacaggc agtccgggcg attgggcgtt
     1261 acctcgacct ccagcacgta ctcgctgatg gggaggcgat ccgccagggc ctctcccgcc
     1321 tcccagtcgt cggggagggc cacgatgccc tcgctcttct gctcgaagcc gagttccgct
     1381 tcggacatga tcatgcccga tgacgccacg ccccgcagct tcgcctcccc gatcaacatg
     1441 ccgttggcca acacggcccc cggaaggcag atggcgaccg tctggcccgc ggcgacgttg
     1501 tgcgccccgc agacgatggt gcgcggcttg tcttcgccca cgtcgacggt gcacacggag
     1561 agcttgtccg cattcgggtg cttctcgcgg gtgagcacct tgcccacgac gaagcgctcg
     1621 atcacgtcgt cacgggcggg gactcccacc agtgcgaccc gctccacctc ggtgccggtg
     1681 agggcgagcc gcgaggccag ttcgtcgacg ggaagatcga tggtgacgtg ctctcgcagc
     1741 cacgaaaggg gggccttcat tgtcgctcca tcctcggggt gatgaccgtc gtctggtgtg
     1801 cgcccggacg aggcgctatc agaactggcg cacgaagcgc aggtcgttct cgtagaacaa
     1861 ccgcaggtcg gtgacgccat gcttgagcat cgcgatacgt tcgatgccca tgccgaacgc
     1921 aaagccggtg aactcctcgg ggtcgtatcc cacgaacccg aacacgttgg ggtcgaccat
     1981 gcccgccccc aggatctcga gccagccgct gcgcttgcac aggcgacatc cgaccccgtc
     2041 gcagatcatg cacgacacgt ccacctctac cgagggttcg gtgaagggga agaagtgcgg
     2101 acgcagccgg atgcgccggt cggctccgaa catggcgcgc gcgaatactt cgagcgtacc
     2161 cttgaggtcg gccagcgtga tgtcccggtc gaccaccagc ccctcgacct ggtggaacat
     2221 cggggtatgg gtggcgtcgg aatcacgccg atacgccttc ccgggagcaa tgatgtagac
     2281 gggcgggcgg cgccgctcca tggtcctcac ctgaacgggc gatgtatgcg tgcgcaacag
     2341 gatgtcgtcc cgcacgaaga aggtgtcgtg cagggagcga gccgggtgat cggccgggtg
     2401 gttcagggcc tcgaagttgt agtagtcggt ctcgatctcg cgaccctcgg ccacctcgta
     2461 gcccatcgag acgaagatgt cctcgatctc gcgcagggtg gaagtgatca agtggagatg
     2521 cccgagcggc atggagacgc cgggtaggga gacgtccaca gcgtcacgct cgagggagcc
     2581 cacgagctct tcggcctcga gcgacacctg cctctcctcg agggccttct ccaggtctct
     2641 cttgatgaga ttgccgaaac ggcctactgc cggacgctcc tccaccgcga gttgcgggat
     2701 gctccgcagc aactgcgtga gttcggcgcg gcggccgaga agacgtaccc tcgcctcctc
     2761 cagcgcggcc agactgacgg cggactcgac cgcggccata ccttcgacat agatggaacg
     2821 caggtcgtct cgttggatct gcgacaggat ggtgtcgttc gctggaatgg tgcctccctt
     2881 cggtgaggga ctacgctagc agaccctcac cggctttgcc aagacgaccc ccggtcagtc
     2941 gctcctgagt cagttcgtac agcaggatgg tgccggccat cgccacgttc aacgagtcga
     3001 atccggcctg aggtatcacc acccggcgcg tgggcgtgga gaactccgga agaccgcccc
     3061 gctcggcgcc cagcaccagg atggccggtc cttccatcga gagatcccgc gcttcgacat
     3121 cgccctgcag atcggcgacc accacctgcg gcacgaggcc gcccctgcga ccctccgcct
     3181 cgagtttggc ggcgatgtca ccgggtgtga cctccaccac gactcgggtg aggaattgcg
     3241 cccccatccc ggcgcgcagc gctttgggcc catacggatc cgcggtgccc gggctgcaga
     3301 tcacacccac tgccccgaag gcgaccgcgc tccgcacgat cgtgcccacg ttgccgggat
     3361 cccccactcc cgcgacgtac acggtgaccc ccgcgtcgat cgccagatcg cccagcgacc
     3421 actcgaccgc ctcgaagaca cacaccacgt ccgcggcgcc gcccagcgcg ctggcctggg
     3481 ccatgacttc ctcttcgcac acgccgatgt cgagttcgtc ctcctcggcc tcgctcagca
     3541 actcggccgg tagccggtcc agcaggtcct ttctcacgag aacctccaca gggagttcgc
     3601 ccgcccgcac cgcagccaac agcaggtcga agccctcgca gacgaacagg gcccggtcgc
     3661 ggcgatgcct cttcttctga agcttctgcg cgagcttgat gctgtcgttg tgtcttcctg
     3721 cgatctcgcg gatcaactcg gactcctctc agctaggtgc tgcggaaccg gatgtgtggc
     3781 gggcggcgca ccgggcaccg caccagccac cgcactcacc accgggcaca gcgcggacct
     3841 ctcacgccgg ctcaaccggt cggcaacgct tcctcgggca gtctgcgagg cgcgggccgg
     3901 cgccgcgcta tcagcgccgc ccccacagtc agtgtgccta cccgcccagc gtacatggtc
     3961 aggatgatca cgaccttgcc tgccatcgtc agcttggagg tgagtccgag cgacagaccc
     4021 accgtgccga aggccgagac cacctcgaag agacctgtat ccaacggtat gtcttgtcct
     4081 gtgtccagga ggagcagcgt catgacaagg atgaacacag ccgaggcaac caccagggtg
     4141 agcgccttcg tcacttgagc ctgcccgatc cggcgagaag cgaactccgt atcccccgct
     4201 ccccggaagg tcgcccgcat ggacgcagcg atgatcccga aggtggtggt cttgattccg
     4261 cccccggtgg attcgggcga cgcgccgatg aacatgagga ttatgaacag gaagatcgtc
     4321 ccgggggcga gccctgcgat cggcaccgtg ttgaacccgg ctgtacgtgg cgtcaccgcc
     4381 tggaagaatg ccaccaggac cctccccggt gcccccaggc cggctaactc gggcgaagtc
     4441 cactcgagcg cgaggaatgc gagcgtcccc accgacagca acccggcggt cacgaggatg
     4501 accatcttgg tttggagagc gtagatacgt cccttccgtg ggcgggcgag gtccaggatc
     4561 acaggaaacc caagcccacc cccgatgatg aggcacgcca ccgtgagggt gaccacccaa
     4621 tcgtcgcgga acccctcgag gctgttcgtg aagagggcga agcccgcgtt gttgaacgca
     4681 cttatcgagt ggaaggcccc gtagcccagt gcacgcagcg ggctgtatcc gtgatggagc
     4741 aggaagtcgc cggctaggat caaggtgccc acaccctcga agacgagggt cattagcagg
     4801 atcgcgagca cgagacgcac cactccggcg agacgcaggt gacccaacgt ctcttggatc
     4861 accagcctgc cttgcaggcc gatgcgtcgc ctcgccagga ggaagagcat ggtggccgcg
     4921 gtcatgatgc ccaggccgcc gagctggatg aggatggcga tcaccgcctt ccccaagggc
     4981 gtgaagacgg tggcggtatc gaccgtaata agcccggtca cgcacaccgc ggacgcggcg
     5041 gtgaacaggg cgtcgatgta ggcgagggag cccggcgccg ccgtcatccc gggaagcgag
     5101 aggacggcgg atccgatgag gatggcggca gcgaaactga ggaggaccac tcggggtggc
     5161 gacagcctca tgtgcatgga tatacccggc ctcgctgccg ccgtccgcgg cgtgttccta
     5221 tgcggtcaga gccgccttgg ctttgcccgc gatctcggcg aagacctgag gttcccgcac
     5281 cgcaaggtcg gccagcatct tgcgattgag ctcgatgttc gcttccttga gaccgtgtat
     5341 gaactggttg tagctcaagc cgtgctcccg ggcgcccgcg tttatgcgct ggatccacaa
     5401 cttgcggaac tcgctcttgc gggctttgcg gtcgcgatag gcgtaggtgt aagacttgag
     5461 cagctgttcc ttcgcctggc gatagttgac gctcttgagg ccgcgatacc ccttagcctc
     5521 ttccaacgcc ttgcggcgct tcttcttcgc gttgacgctg cgtttgatgc gtgccatcgg
     5581 tcggccctcc tagattccca gaagggcgcg gcagtggccc agattggtgc tgtccaccgc
     5641 aacgtcctta cgaaacttcc tcttgcgctt gctcggcttc ttcgtgagga tatggctcga
     5701 gaaagcgcga cggtggacga gtttgcccgt cgcagtgcgc ttgatccgct tcttcatgcc
     5761 cttatgagtc ttcttcttca tccgcatccc tgctgtcgtt cgcttctaca gttcatctcg
     5821 aacgccgctg tgggcgctcg agtcgcaccg agccgttggc ctggtgccac gacggaatca
     5881 agatcggcag accggccggg gccgcctagt gcttcttcgg cgccaacatc atgatcatgt
     5941 tgcgtccatc gagattcggc tgactctcga cgtcacccag ttcttggagc tcctcggcca
     6001 tccttctcag aagacgctcg cccaagtgcg ggtgcaccag ctcacgccct cggaacatga
     6061 tggtgagctt gaccttgtcg cccttggcga ggaaccgctc gacgtgcctc ttcttcgtag
     6121 cgaagtcgtg atcgtcgatc ttcggccgca gcttgatctc cttgatgctg atcgtcgtct
     6181 gccgcttgcg cgcctctttg gccttctgtt cctgttcgta tcggtacttc ccgtagtcca
     6241 tgaccttgca caccggtggg ctggccatgg gggccacctc caccaggtcg aggttcagac
     6301 gatcggcata ctccgctgct tcgcgcagac tgacgatgcc gacttgctcc cctgcgggac
     6361 ctatcaaccg taccgattcg gcgcggatcc gatcattgat tcgagtggtc tcagctatct
     6421 tgctcaacac ctccccggca gtgccggatc gatctgccta cacatgcacg aaagcggcag
     6481 cctctggcca ccgctccact tccgcctttg gcggatacgg atatgcatga agccgaccac
     6541 gttgcggccg cgcctcgcgc attctccatg ttggacccgg caccttactt ggcgcgaggt
     6601 gggaagagtg gtttccactt tatgtgcagg cggcagtata gcccaccgta ctcgggttct
     6661 caagcacgca aggccatcgt cgtgaagacc gcaccgccgg attcaagcga cgatcacggg
     6721 catgcgtgac gccgccgcaa tctcgtcgat cgccagagag cgggcgcggg ttcccagatc
     6781 gccgtcggcg tgagagcgga gcgagacggt gtcaccctgc gactccctgt ccccgacgac
     6841 caccatgaag ggcaccttga gaacctcgtt gtcgcggatc ttgcggccca tggactcggt
     6901 gcgatcgtcg acctccaccc tcaagccgac cgcggcgagc gccgccgcgg tctgccgcgc
     6961 gtactcgttg tgcctgtcgg ccacggggat gaccaccgcc tgcaccggcg cgagccaggc
     7021 cggaaaggcg cccccgtagt gctcgatcag gatgcccaag aagcgctcga tgcttcccag
     7081 gacggttcga tggatcatga ccggacggtg gcgctggtta tcgtcgccca cgtactccag
     7141 gtcgaagcgt tcgggcatgg cgaagtcgag ttggcaggtg gcgcactgcc aagtgcggcc
     7201 catcacatcc tcaacgtgga agtctatctt ggggccgtag aacgccccgt cgcccgggtt
     7261 caacgtgtat tcgaggccgg cgcgatcgag cgccccggcc agagccttct cagccgtctc
     7321 ccacatctcc gcactgccga tggacttctc cggccgcgtg gagagctcga ggtgcacgcg
     7381 atcgaagccg aaggtgcggt agatcttgcg ggtcaactcg atgaccccca ccacctcgtc
     7441 ctcgatctgt tcgggcaggc agtagatgtg cgcgtcgtcc tgcgtgaacg tgcgcacccg
     7501 gaagagccca tgaaggactc ccgagagctc gtggcgatgg accaagccga gctcggccat
     7561 ccgcaaaggg aactcccgat acgagtgctg ttcggcacgg tagatgagta tgccccccgg
     7621 gcagttcatc ggcttcaccg cgtagtcggc gtcgtcgatg cgggtgaagt acatgttgtc
     7681 tttgtagttc tcccagtgtc ccgactgctc ccacagcgcg cggtcgagga tgatgggcgt
     7741 cttgatctct tcgtagccgg cggcgcggtg ctccgcgcgc caatagtcga gcagcgcgtt
     7801 gatcacccgc attcctttag catggaagaa ggggaagccg gggccctcgt cgtggaagct
     7861 gaagagatcg agttcccggc ccagcttgcg atggtcacgc aggcgagcca tctcgagctt
     7921 ctcgaggtgg acgtccaaat ccttcttcga ccaaaaagcc gtgccgtaga cgcgggtgag
     7981 catggtgttg gcggagtcgc cgcgccagta cgcgccggcg atcgacaggg ctcgaacggc
     8041 ggggatgcgc cccgtgtcgg gaagatgcgg gccgcggcac agatcgacga agtccccgtg
     8101 gcgatagagc gagatggtct ctccctgagg aaggtcacgg atcagctctg ccttgtacgg
     8161 ctcgccggcg aacaattcca gggcctgttc cctcgacact tcctcgcgga cgaaaggccg
     8221 ggcttccttg atggaccgct ccatctcggc ctggaacctg gcaagatcgt cttcggtgac
     8281 cggctcgggg aactcaaaat cgtagtagaa gccgtccttg atcgcagggc cgatagcgac
     8341 cttggtgccc gggaacaccc tcgtcgccgc ctccgccagc acgtgcgcgc tcgagtgacg
     8401 caacacctga aggccttctt ccgacgcggg agtcacgatg ctcacctggt caccttcatg
     8461 cacgggcgtg gcaagatcaa ccagtctccc ccccacggtc gcggccaccg ccgctttggc
     8521 caggcgcggg ccgatagtct cggccaccgt gagtgcggtt gcaccttccg ggacggagag
     8581 ctttgtacca tcaggcagga tgacgtccat gagtcctcgc tcgaagccgg ggtcgcaacg
     8641 ggcggaccgt ttgccgcccc gccgatgagt atagcaaccg actgccggcg gacgttcgtc
     8701 caccgaccgg gccctcggtg cgccgcccag tgcgcctggt cgggcagccg gctgcgcatc
     8761 catcggcgat aaacaaaggg agtcccggca gggactccca gaaacaagtg ggcgatactg
     8821 gatttgaacc agtggcctct tccgtgtcaa ggaagcgctc tccccctgag ctaatcgccc
     8881 gatcagccgg gacatagtac agccgcctcg accgcgcggc aacccgaacg ggacagtttc
     8941 tagcggtaca catccaccgg catgccgacg ggcagcagcg gatagatggc gtcggtggcc
     9001 cagtagggga tacgcacgca cccgtgactc gccggatatg tggggatcga gtacgaccca
     9061 tggatcgcta tccccccgta gaagtaggag ggtttgtaga gcaggcccag ggaactctca
     9121 cgccaagcgt ctatcttcct aagaatgcgg aagcttcccg ccggtgtggg ggtgctcgag
     9181 gcgccggtgg acacggccag gatgcgcgtg acccggttcc cggagatcag gaagagcacc
     9241 tgtctcgtga gggcgatctc tacgcgctca cccgccgccc agttgcgtgg gctcggcgtg
     9301 accgccgtgt ccagccgctt ccagacactc gggcccaccg tgcctgtcct ttcgagcccc
     9361 tccactttct ggaaagccct cacagcatcg cggagggcct cgtcgaactt cgcgtcggcg
     9421 acacccacag cgtacttcag cgccgccaaa cgttgctcca gcaaagcgac gtaggcgccc
     9481 gtggagccct gctgcaaggt cacttggggg atcgccggca ccggagccgg ctgtgtggtc
     9541 aaaggcacca acggctggta cagggcacga tagaggagga ccgctgcctg cgcccgcgtc
     9601 agcgttccga gcggcatgaa gcgcccgtcc agggtgccct tcacgacccc caacctcact
     9661 gcgtcggcaa cctcagcgcg gtggatcgcg tagatatcgt agcgatcgct gaacccggcc
     9721 agccagccgt ctacctgtgc cgagtcgaga gcgatctcca caccctcttg aggctcggtc
     9781 tggaaacgat agacaagcgc gcggatcatg agcgtggcca cctgctggcg agggatccct
     9841 aggctcgggg agaaggtggt gcgagttgtg cccatgagca ggcccgcctc gtagagcgag
     9901 gcgaccgggg cctggaacca gtcggtgtcg gacacgtcat cgaagggctc gtcaacagcc
     9961 tcgggcgtcg ccaaggcccg cgagagcatg gcggcgaact gagcgcgcgt aaccgtttgg
    10021 tacgggccga aggtgccacc cggcaggccc atgatcacac ccgcgtcgct cagcacgtcc
    10081 accgcttcgt agaaccagtc cgtcggctcc acgtctggga agggaagagc cgacgccgcc
    10141 gagactccga caccgaggag caacaacgct gcaagcgtcg aaacgatcat tgctctcgcc
    10201 gcgattcgtg ccaccaatgc cacctccacc gagtctccga caccgcccga actacgcgcg
    10261 aaggcccgcc gcagcggacc tcgatctatc tggtaaagag gcgccgaccg gagtcgaacc
    10321 ggtgtagcgg gttttgcaga cccgtgccta agccactcgg ccacggcgcc tcgtgcctgg
    10381 cgcgcacaag aaaagcggga ctcatgagcc ccgcgtcgcc gcaatgaggt tggagcggat
    10441 gaagggattc gaaccctcga ccttctgcat ggcaagcaga cgctctagcc aactgagcta
    10501 catccgcccg ctccatccca cggccactgc ggccccgccg agcatcgccc ggcgggtcag
    10561 gattatagcc gagcctccag gcagatgcaa accgatcccg cgaccgctct tcccccatcg
    10621 cgccaggcct cacgtgttgg gggatcccac cggccactga ggctagcgcc ctccgtcgag
    10681 agcctgattg gcgagctcat ccggccgcga gttctcctcg cgagccacgt gcactatctc
    10741 cacccgttcg aagcgctgga ccaacgcgcg ggcctgcagg tacagctcct tcagctgttc
    10801 gctcttgact ctgtactccc cacggagttg cctcaccagc agctccgagt ccgacagtat
    10861 cctgagccga ccgatgctcc ggtcgagcgc agtctccaag ccggtgatca acgcctggta
    10921 ctccgcctga ttgttggtcg ccaccccgat gcgcgccccc aactcctcga cgatctcccc
    10981 ctgctcgtcc tcaagcacca cgccgatcgc acccggcccg gggttgcctc gggaggcgcc
    11041 atcgactcgc aaggtgaagg tgcgagtggg ttcgccgttg ggcgcgtcgt cactgtccgg
    11101 gccgccgacc cgttccccgc ccgcctcctc gaggtcgaag agccgcacga cgccgttcgc
    11161 ctcgcccgca tgagacacgg acttggccgc gtgccacggc gaccgacccg cttgggagaa
    11221 gacgacctcg acgtcgtgct ccgcgacagc ggctccgaaa ccctcacacc agcggcgcaa
    11281 ggcccacgtc tccaagtgcc cgtgcgggac cgcgaccagt gccaaacccg catcggccgc
    11341 cttctccgca tcgtggtact tcaagtcccc ggtaagaagc acgtcggccg agcgcgcggc
    11401 ctcagcgatg aagctggagc cgctccccgt gacgacggcg acgcggtcga ccatgtgttc
    11461 gatatcgccg gcgaagctca cctccggcag accgaagacc tcggctgcgg tctcggccag
    11521 cgcaaccagc gacacctcgc tgcgcgtgga gcccactcgc ccttgtcccc cggtgaggga
    11581 gacgttctcc accgggtaca catcgaacgc gggttcctcg taagggtgcg cgcagatgaa
    11641 cgcctgcaca gcggcgacca ccctgttctc cggaacgatg gtctcccacc gcacctcggc
    11701 gacacggaca ctctcaccag cacggcctac cgtcggatgc gtggacggtc ctgccacgaa
    11761 cgtgccctcg ccgcgattct cgaaagagca tccttggtag tcgccgatgc gtccccctcc
    11821 agcttcgaac acagctcggg agacacgttc gacctcaccc tccggtatga atcccaccaa
    11881 cttcttgact cgcgacgggg tgcgagcgag cgggagtatg tgcgccagac ccatctccct
    11941 ggccaccagg tcgcagaggc caccgggagc accatccaga ttcgtgtggg ccgccagcag
    12001 ggcgacgtcg gcggctacca actgtgtgat caggacaccg actctgtcgg cgtcggtgac
    12061 gcgggtgatg ggagcgaaca gcagaggatg gtgcgtgatg atcgcctgga agccctctac
    12121 tacggcttcc ttgacgacct cctcggtgag atccaaggcg acgaggacgc gccgcagctc
    12181 cgtgccccgc cgccctacga ggaggccgac gttgtcccaa gactcagcca gcgcccgcgg
    12241 cgccgccagt tcgatcatgc gctccaggtc ttcaacgagc atcgaggctc cccggttgcc
    12301 gagtggtcgg gcggcatcgc tctactgcca gtatcgggca aatgcagcgg ccagtccagg
    12361 ggcgacgtgt caagtgcccg cgctaccggg ggcggttcac cactcctggt agtcattctt
    12421 ccttcttggg gtgtccatgg ggacggggcg aggtcaccgt ggacacgatg aggtccacgt
    12481 agctgttctt ctctacctcc accccggcag tcggactctc cccgatgaca aggttgggcg
    12541 gatagtcccg tgtctcccga aggacgatcc tcgcgcgcag actgtacgtg gccaacatgg
    12601 ccgtggcctg ctccggcgag agccccacga ccgcgggcac tatcaccatg gtcggcgcgt
    12661 tgcttatgga gatgatgacc gtagatccgg gctgcgcgtc tgtacccttc ttggggttct
    12721 gatccactac cgtgccgccg gtgagcgtcg atgccactgg ctgcaccgtc gccaccaact
    12781 tcatgccctc gatcagatct acagcggcgt cctggttcat gccgaccacg tcgggcgtct
    12841 tgaccgtgcc cttgcccgag gacagccaga cactgaccga cttgcccttg gctagcttcg
    12901 tgctctcttc ggggtcttga cgggcgacgt caccggcctt cacatcggcg gacggctggt
    12961 ctccagcgtt cttgaagacg aagcccgccg cctcgacctt ctgcctggcc tcagcttcgc
    13021 tcaggcccat gactccaggc acgaccacca gatcggtgcc agcgctcccg aaattggaag
    13081 aaatcgcgta tgcccccccg gccagcgcca aaataaggac gacgaccaga atccagggcc
    13141 agacgctgcg tcgccgaggc ggttcatcgt gctgaggctc cggggcacga tccagggcgt
    13201 ccgacacctt cgggcgcact tgggtgggct gagccacgaa agccgcggcg gcaggactca
    13261 tgaccctagt ggcctcttcc ctgaagacgg caggcgcgct gatcgcctgt ccgtcctgga
    13321 ccttgcgtag atcgtcgagc atggcctggg cggtgaggta gcgttcggtg ggcttcttgc
    13381 ccaaagagcg cagaatgacc gcctccaggt cctcgggggc atcgggggcg atgttgcgcg
    13441 gggggatggg gtgctcgtgc acatgttgcg tggcgatgct caccggattg tcgccgtcga
    13501 acggcggcct gccggtggcc atctcgtaca tgaccacgcc cagcgaatag agatccgacg
    13561 cggcctcgac gggctgcccc tgagcctgtt cgggtgacag atactgggcc gtccccagga
    13621 tagacccggc ttcagtcatg gtagaggcgc taccggcccg ggcgatgccg aaatcggtga
    13681 ccttggcccg gccgtcgttg tcgatgatga tgttctgcgg cttaatgtcg cggtggatga
    13741 tgtcccggcg gtgggcggag cgcagcgctt cgaggatctg cacggagatg gagatcagga
    13801 ggtctgaact gagcggcgcg catgtgagga tgatctcctt gagcgatcgc ccctccagat
    13861 actccatggc gatgtagtag gtgccttccg cctctccccg gtcgtaaatc tgcacgatgt
    13921 tggggtggtt gaggccggca gcgctgctgg cctcgcgacg gaagcgctcc acgaactgct
    13981 cgtcgtgcgc ataacgagtg gagaggatct tgagagcgac ctggcggccc aggagtcgat
    14041 cgttggccag gtatacatcg gccatgccgc cgctacccag cttgcgcgcg atctcgtagc
    14101 gatcttcgaa cactcgtcct atcaaggtct taccgtcctt cggcacacct ttgtgcccgt
    14161 ggtcacgcgc gccgttgcga ggtcgtaagc gtggaatggg agttcagaat agcccaggtg
    14221 cgctgttcgg caactccgag tccaagacaa cggctgccag ctactctcgt tccccgggag
    14281 cgtcgaccgg tacgaggagt cccagctcgg ccacatggtg aagagcgcgg gccagttcgg
    14341 ccgcttcctt ccagcttacc actcgcccac gcttccgcag ggcagcagcc agcccgtctc
    14401 caaactgagg gccctgccta tcgaccgcca ggtcctctcc tcaatccggc gcgcttgctt
    14461 ggttcttccg ttgcgatata ggaatcatct tggcttcttt gaaagacaca ttgaatggta
    14521 ggccgaagct caccttcacg ttggtgatac cggccacttc cataccctga attactttgc
    14581 atccgcatga actaactgca tcacgcaccg tctctgccgc agaatcgcag gccaatacca
    14641 caatcgcatc gtagtccttt gcgtggtccg ccagcttctt acgcttgctt gttggccaca
    14701 tgcataagaa caagcttcta tataactttg ttctgacatc tgctcttgtt agttctgatt
    14761 gaattccttc catgtactgc tcgaaa
//
LOCUS       DASRMJ010000029         4942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_508053, whole genome shotgun sequence.
ACCESSION   DASRMJ010000029 DASRMJ010000000
VERSION     DASRMJ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508053"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            639..1643
                     /locus_tag="VFD74_00775"
     CDS             639..1643
                     /locus_tag="VFD74_00775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZK48118.1"
                     /translation="MRKPPRLAIVVPCYNEEEALPETSTRLDALLVELTGAGLISCES
                     FCLLVDDGSRDATWSVIEEWHERSPRIAGLKLAHNAGHQKALLAGLEHVAGGVDCAVS
                     IDADLQDDPAVIGEFITKYREGCDIVLGVRRERATDTRFKRWTAQGFYRLMRMLGADV
                     VYNHADFRLMSRKALGFLGRFGEVNLFLRGVVGLIGLPSAVVYYDRRARFAGESKYPL
                     TKMLAFALEGITSFSVAPIRLVTYVGFSFCLLALAAAVYSLASLASGVAEPGWTSLIL
                     SVWFIGGVQLISLGLLGEYVGKIYQESKGRPRYLVETSLLADVESGEPCSGGPEEGLS
                     "
     gene            1667..2914
                     /locus_tag="VFD74_00780"
     CDS             1667..2914
                     /locus_tag="VFD74_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HZK48119.1"
                     /translation="MRRAREWTVGAVSAVRDLTPGFLRARTNVGLALQVFVMNKFLVV
                     LVSMVANWEGWRRLLSVDMAYEVLVRNYRFFDAGWYVGIAANGYVHPKETAFFPLFPL
                     TMRLFGWVLPVGFLAAGVLVSNLAFFGLLYGFARLARHDLDEQTARRAVWLLGLYPTS
                     FYFSAAYTEALFGLATVLALYSMRRRRWALAGVAGGAAALTRNVGVLLVLPFLLEYWS
                     SLREGSAKPGKHPVSRSRGRRSLASVLWVGVIGAGGLLYVAFLRWRFGEPFAFVNTQS
                     LYGRESLMPWSTLYRGYRFGLQQIAAVDLPITWHDLYFTTQLFFVTLVLVVLVASVGR
                     MRWSYWVVILYSFTIPMVGPSTEGVVDYFISFSRYSVVILPLFLGLAGLLRNRRLCRA
                     YVVVSVGLLVLFVYAWSVGTWVA"
     gene            3308..4342
                     /locus_tag="VFD74_00785"
     CDS             3308..4342
                     /locus_tag="VFD74_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48120.1"
                     /translation="MEALIEALKEESLRPDDDAPAEARLLRDEEGATSMRSALGAARP
                     SGRGAPARPMKEGLRHLFSPTRVAPAGPRRAGPMTDSNHSSHYEAFLRRAALRSHVIS
                     SGYERLDDLMGGGLRPGLHFLTGHHTRMRRAFLDNLMWGAVEHKRPIWYCALDTGAQA
                     VWERMIVALGYLLGEPVLPQELRASSGVDDVGDRVGRIDAALVEGVFPYVWLRDSVEM
                     AEQGSPGQFLASLDAWLTCEDSPRLLVIDSFFTLSTLLSGDEEAANGVDLAGELDRLL
                     RKRSSAAVATVPVGPDALLCDVGRGYLRLGELEGSGDGTAESVLVTARDAHGVRSQVF
                     FVDGVAGIFG"
BASE COUNT          733 a         1581 c         1656 g          972 t
ORIGIN      
        1 ctagaactgt ctcctaacga gtgcaataca ggtcttcggg gtggggcggg gcaaaagatc
       61 accatcgcgg gatagtgcga cagtggtgcg ttccctcagt gatccccccc gagagccggc
      121 cgaacgaggg gaccggccgg ctgtgaacta ctcaactcct gcgtcgacgt agcccccagc
      181 tctcgaactc cccgtaggtg ttccgcaagt tcgtggaggc ggctgtagct cctaaggacg
      241 ctggtgtcct tgagttgcgc gtcggctggt gaaccggaga gagggcggtc cgcacagcct
      301 gaacgagtct cttcagttcg gcgaattgcc attcggagag atggctctgg cgaccagact
      361 tggagactgc gatcgggtcg agggtcagtg ggagctgccc cccgtctggt cttggactcg
      421 ctagtgacca cctcggcgtt caccgtcact tcttgatcac tttgcgtccc acccctagaa
      481 gcggtctgaa acagaccggg caccaactcg gatcccccgg ccaacgccgg caccccacgt
      541 gcccgttgcc gatgccgtgg tcatttggta gtgtcggcgg acgcggctgc gtgctgccgg
      601 tgccctcttc cacctcggac agtcgacggt tggatgtgat tcgaaagccg ccccgtctcg
      661 ccatcgtcgt gccttgctac aacgaggagg aagcccttcc cgagacgagc actcgtctcg
      721 atgcgcttct tgtggagctg accggtgcgg gtctcatctc gtgtgagagc ttctgcctgc
      781 tcgtcgacga cggcagccga gacgccacgt ggtcggtcat cgaggagtgg cacgagcgga
      841 gccccaggat cgcgggcctc aagttggctc acaacgccgg ccaccagaag gctctgctgg
      901 cgggcctgga gcacgtagcg ggtggggtgg actgcgcggt ctccatcgac gccgatctcc
      961 aggacgatcc ggcggtgata ggggagttca tcaccaagta ccgcgagggc tgcgacatcg
     1021 tgctgggggt gcggcgcgag cgcgccaccg acacccgttt caagcgctgg accgcacagg
     1081 gcttctaccg actcatgcgc atgctgggtg cggacgtggt ctacaaccac gccgacttcc
     1141 gcttgatgag ccgcaaggcg ctgggcttct tgggccgatt cggcgaggtg aacctcttcc
     1201 tgcgaggagt ggtgggcctc atcggacttc cgtcagccgt cgtctactac gaccggcggg
     1261 cacgtttcgc gggagagtcg aagtacccgc tcaccaagat gttggccttc gcgttggagg
     1321 ggatcacctc tttcagtgtg gcgcccatcc gcttggtcac ctacgtaggg ttctcgttct
     1381 gcctcctcgc cctggccgcc gcggtataca gtcttgcgag cctggcttcg ggcgtggcgg
     1441 agccgggctg gacctccctc atcctctccg tctggttcat cggaggagtc cagctcatca
     1501 gccttggcct tctgggggag tacgtgggca agatctacca agagagcaag gggcgccccc
     1561 ggtacttggt ggagacgtcg cttctcgccg acgtcgagag tggagagccg tgctcaggcg
     1621 gtcccgagga gggcttgagc tgaacccgtc cgggccaggc gtggggatgc ggcgtgcgcg
     1681 cgagtggacg gtcggggcgg taagcgcggt cagggatctc acgcccggct tcctcagggc
     1741 acgcaccaac gttggtctcg ccctccaggt gttcgtgatg aacaagttcc tcgtcgttct
     1801 ggtgagcatg gtcgccaact gggagggctg gcggcgcctt ctctcggtgg acatggccta
     1861 cgaagtcctc gtccgtaact atcgattctt cgacgccggc tggtacgtgg ggatcgccgc
     1921 caacgggtat gtgcacccga aggagacggc cttcttcccc ttgttcccct tgacgatgcg
     1981 gctgttcggc tgggttcttc ccgtgggctt cctggccgcg ggggtgctcg tttccaacct
     2041 ggccttcttc gggcttctct acggcttcgc acgattggcc cgccacgatc tcgacgaaca
     2101 gaccgcaagg cgcgccgtat ggttgctcgg gctgtacccc acctccttct acttctcggc
     2161 cgcctacact gaggccctct tcggcctggc cacagtcctg gcgctctact cgatgcgcag
     2221 acggcgctgg gcgctggccg gcgtcgcggg aggggccgcc gctctgacac gcaacgtggg
     2281 cgtccttctc gtccttcctt tcttgctgga gtattggagc agccttcgcg aggggagtgc
     2341 gaagccgggg aagcacccgg tctctcggag tcgcggacgt cgcagcctgg cgagcgtgct
     2401 gtgggtgggg gtgatcggag ccggcggcct actctacgtg gccttccttc ggtggcgttt
     2461 cggagagccg ttcgccttcg tcaacacgca atcgctttac ggacgcgagt ccctcatgcc
     2521 gtggtccact ctctaccgcg gctaccgctt cggcctccag cagatagcgg ccgtcgacct
     2581 gcccatcacg tggcacgatc tctacttcac cacgcagctc ttcttcgtga ccttggtcct
     2641 ggtcgtgctg gtggcctccg tcgggaggat gcgctggtcc tattgggtgg tcatcttgta
     2701 ttcgttcacg atcccgatgg ttggaccgtc caccgaaggc gtcgtcgact acttcatctc
     2761 gttctctcgc tactcggttg tgatcctgcc gctcttcctc ggcctcgccg gactgcttcg
     2821 gaaccgccgg ctctgccggg cgtacgtggt ggtgtcggtg gggctcctcg tattgttcgt
     2881 gtacgcctgg tcggtgggca cgtgggtcgc ctagtctcgc gcccgcgctc gcgggtagga
     2941 cacggagcct tggccgagaa ctatagtgct gttggcgggc gggagggaga tggccaagtc
     3001 ggagggcggg cggtacgacc gcccggcgtg ggacgatgag ttcgaggtag ccttcgggag
     3061 cttgacggtt ccggcggtcg aggtggccgc gcggcactcg atgctcgggc cgacagaccc
     3121 cgcgcgtgag acgcagtcag cgccgacctc cactccaggg caacgggagg gcgaggggcc
     3181 ggcaccggcc cacccgccac tcgccgaacc tgaaccggtc gtaatcgcgc cggccgaacc
     3241 acttctggcc ggagcgctgc cggaatctct tgaatcagtt gtgacggagg ccccgaagga
     3301 ggtactgatg gaggccctga tcgaggccct gaaggaagag tcactgcgtc cggacgacga
     3361 cgcgccggcc gaagctcgat tgcttcggga tgaggaaggc gccacgagca tgcggagcgc
     3421 acttggagcg gcccgtccct ccggtagagg agcgcccgcg cgaccgatga aagaagggtt
     3481 gcgtcatctg ttctctccca ctcgggtggc acccgccggc cctcgtcggg cggggccgat
     3541 gacggattcc aatcactcct cgcactacga ggcgttcctc cggagggcgg cgctccgttc
     3601 ccacgtcatc tcgagcggat atgagcgtct cgatgatctc atgggcggtg ggctccgccc
     3661 gggcctgcac tttctgaccg gacaccatac ccggatgcgg cgggccttcc tggacaacct
     3721 catgtggggg gcggtcgagc acaagcgccc catctggtat tgcgccctcg acacgggagc
     3781 acaagcggtc tgggaacgaa tgatcgtcgc tctcggctac cttcttggcg aaccggttct
     3841 tccccaagag ttgcgcgcat cgtcgggggt cgatgacgtg ggcgatcggg tgggacgcat
     3901 cgacgctgcc ctggtggagg gcgttttccc ctacgtgtgg ctccgggact cggtcgaaat
     3961 ggccgagcag gggagccccg gccaatttct ggcctcgctg gacgcgtggc tcacgtgcga
     4021 ggacagcccg cgcctgctcg tcatcgactc cttcttcacg ctgtccaccc tcctgtctgg
     4081 agatgaagaa gccgccaatg gagtcgatct cgccggcgag ctcgaccgac tcctacggaa
     4141 gcgatcgtcg gccgcggtgg caacggtgcc ggtcggtccc gacgctctgc tctgcgatgt
     4201 ggggcgaggt tacctgcggc ttggggaact cgaaggctcg ggagacggaa ccgcggagag
     4261 cgtcttggtg actgcgcgcg acgcgcacgg tgtcagatcc caggtcttct tcgtcgacgg
     4321 agtggccggc atcttcgggt agccgatctc ggccgaccga agcggccgct ccgggaatac
     4381 gctagtgtca cccgccatcc tcgcccgctg gctgccgccc accgcccgac tcctgccggc
     4441 tgcggtcggt catttgccga cggattctgt gggggcggcg acgtgccgaa ggcggatcgg
     4501 cgcgaaaggg ttgagagatt aacctttctg acgatatagt gaattggaga gttcacccgg
     4561 ttggttccag ccgcccggag tggctttctc ctctcagcgc cccccgggcc catcccccag
     4621 ggggcgctcc tcgctcttct cgctgaaagg tggtcatgcg tccgtttctc cgcgggagtc
     4681 cacactcctg cactctgcct cgcggcgcgt ggccttctcg gccggggcgc gtcgcttgta
     4741 ccggcttggg gcactacgct agagtgcacc ggggaactgt gaagcatgac gtcacggagg
     4801 gcagagatgc cggacgcccc gggcccattt cacgacacct cgtcggccat cagcctgaac
     4861 gaccgggcgg ctgctatcgt tgagcgcatg atcgccgaag cgccgagcga cagcatcgct
     4921 gctcatcggc tcgtcaacgg cg
//
LOCUS       DASRMJ010000030         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_2242, whole genome shotgun sequence.
ACCESSION   DASRMJ010000030 DASRMJ010000000
VERSION     DASRMJ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..740)
                     /locus_tag="VFD74_00790"
     CDS             complement(36..740)
                     /locus_tag="VFD74_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZK48121.1"
                     /translation="MRCTCEEIAAGTEGLSPICFAHLWLFQNLPERDAKALTEAAWRK
                     QYQAGELVFLQGDQARIMFIIKAGRVKLAKAGTDGGEVLVDIRKAGDFLGETFLSDDA
                     EYPLTAECMEESLLCGFTKARFERLVTDNPNIGLQIIRNMSDRISWLTSRASSTSATH
                     LEECLHAVLTDLAHEHGTITEKGLLIQFPLTHAELGFLVGAHRVSVTKALKALRESGR
                     VIRQGKHLVLPGRQSQ"
     gene            complement(811..1356)
                     /locus_tag="VFD74_00795"
     CDS             complement(811..1356)
                     /locus_tag="VFD74_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HZK48122.1"
                     /translation="MRLKVLGCSIFRKEIEYLRGTDFAVAWLPAGLHVSDERLGAALG
                     QALAGEEEVACFYGACHPDMNLLLAGHGGRRLQARNCVEAFLDPVERARLGERVFIMT
                     PGWLREWRSIFIEGLGWDEIDGRINFGGYDKIVVLDFGLEPIDELAVLEFYDYTQTPV
                     ETVPASLGWFRARVEEVLGEE"
     gene            complement(1438..1674)
                     /locus_tag="VFD74_00800"
     CDS             complement(1438..1674)
                     /locus_tag="VFD74_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH865 family protein"
                     /protein_id="HZK48123.1"
                     /translation="MDAKEDIKQQIAGALAGASFPIASPEELIGAFPNGADTMCVSGD
                     VKLRAGDAGTVLTAADFPFTSAEQVADTIVSRAL"
     gene            complement(2066..2683)
                     /locus_tag="VFD74_00805"
     CDS             complement(2066..2683)
                     /locus_tag="VFD74_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HZK48124.1"
                     /translation="MAGFAYEKRVARCGELIRAQELDVLLLAKPSNMFYLTGDGRLCA
                     YAMLTQEGKVALGVLSTDVADVSVLARFDRIAGFEDEVGMLHAIAHDFREFGLESGVV
                     GLEYSFLTQSMLRMLTHPHAKPAGMEPKDATPVLSQLRLVKEPEEIARMREAGRVWPA
                     SAWLPPSRRPGSTPCAWPAPRSSGAPMYPGVRAPTSPTDSPPVGR"
     gene            complement(2757..3095)
                     /locus_tag="VFD74_00810"
     CDS             complement(2757..3095)
                     /locus_tag="VFD74_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZK48125.1"
                     /translation="MKIVTASEAPETVNPHGVSARPLHSTEHVQVSMITLRPGEALKL
                     HSTPVDAFFYVLEGSGTVEIGDESEGVSKDMLIESPAKIPHRLLNEGDETFRFLVVKT
                     PRQADPTRLL"
     gene            complement(3323..>3533)
                     /locus_tag="VFD74_00815"
     CDS             complement(3323..>3533)
                     /locus_tag="VFD74_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48126.1"
                     /translation="ILERGQSVGRVRTDIDIEVMIDLLIGPLLARLVTGGRADQEAVT
                     ARINALWPALSPSSESSSPKAESNI"
BASE COUNT          604 a         1163 c         1086 g          680 t
ORIGIN      
        1 ctggccggcg ccgaggctct actctgccgc ttccgctact ggctttgccg gccggggagc
       61 acaagatgct tcccctgccg gatgaccctg ccggattctc tgagcgcttt caaggctttg
      121 gtcacgctca cgcggtgagc cccgacgaga aagccgagtt ccgcgtgggt gagtgggaac
      181 tggatgagca gccctttctc ggtgatcgtg ccgtgctcgt gggccaggtc ggtcaagacc
      241 gcgtggaggc actcctccag gtgagtcgcc gaggtgctgc ttgcacgact cgtgagccag
      301 gagatccggt cgctcatatt gcggatgatc tgcaggccaa tgttcgggtt gtcggtcacc
      361 agtcgttcga aacgcgcctt cgtgaagccg cagagcaggc tctcctccat gcactccgcg
      421 gtcagtggat actcggcgtc atcactgagg aaggtctctc cgaggaagtc ccctgctttg
      481 cggatgtcga ccaggacttc gcccccgtcg gtccccgctt tcgcgagctt cacgcggccg
      541 gccttgatga taaacatgat ccgagcctga tccccctgga ggaaaaccag ctcacctgct
      601 tgatactgct tgcgccaggc ggcctcggtg agcgcttttg cgtcccgctc gggcagattt
      661 tggaagagcc agaggtgggc gaagcagatg ggggacagac cttctgtgcc agcggctatt
      721 tcctcacagg tgcagcgcat gccgccaaac tccttcgctc tgttcgccgt cagatgccgc
      781 gtgatcctcg gttctcgtca tgagcagata ctactcttcc ccgaggacct cttcgactcg
      841 cgcccgaaac caacccaggg atgccgggac tgtctcgacc ggtgtctgcg tgtagtcgta
      901 gaactcaagg acggccagct catcgatggg ctcgaggcca aagtccagca ccacgatctt
      961 gtcgtatccg ccgaagttga tgcgcccgtc gatctcatcc caccccaggc cctcgatgaa
     1021 gatgcttcgc cactcgcgca gccagcccgg ggtcatgatg aacacccgct cgcccagtcg
     1081 tgctcgttcc accggatcca ggaacgcctc cacgcagtta cgcgcctgca ggcgacggcc
     1141 gccatggccg gcgagcaaga ggttcatgtc cggatggcag gctccgtaga agcaggccac
     1201 ctcttcctcg cccgcgagcg cctgtccaag tgcggctccc agccgctcat cgctcacgtg
     1261 aagaccggcg gggagccaag cgacggcaaa gtctgtcccg cgcaggtact caatctcctt
     1321 gcggaagatg ctgcagccga ggactttcaa gcgcatggcc cccggtcatt cggattgggg
     1381 gggcacgatg cccccccgca ttgccgcgag tcgacctcta ttctcccagc gggcccctta
     1441 gagcgcccgc gacacgatcg tatcggccac ctgctctgcc gaggtgaaag ggaaatcggc
     1501 cgctgtgagg acggttccgg cgtcgcccgc ccggagtttg acatcaccgg agacacacat
     1561 ggtgtcggct ccgttcggga aggctccgat cagctcctcc ggactagcga tcgggaaact
     1621 cgctcccgcc agtgcgcctg ctatttgctg cttgatgtcc tctttagcat ccatgaccta
     1681 gacctcctcg atagctttct cggcggccag ctgctcgcct gcggacatgg tacagggctg
     1741 gtcgtatgac tgttgtagcc atggctacat tcgagcgggc atcgcagaga aacggtgaga
     1801 tcagactccg gccgatcggg cgttcacccg cccgggtgag atgatgaacg gatgggacgg
     1861 cggaacgggc tgggtactta tgtggttgct cgtattcctc ttcgtggccg ccttcatcgc
     1921 cagcgtcgta ctcctggttc gggctgctgc tcactagcag ccgccatggc gaggttgtgg
     1981 cgttcagatg gtcttccctt cgagaatcag gcggtaccag ggagacgatg catcaacgag
     2041 tcaatcatga cgagatcgcc cgactctagc gcccgacggg tggggaatcc gtgggcgatg
     2101 ttggtgcgcg caccccagga tacatgggtg ctccagaact tctcggcgcc ggccaggcgc
     2161 atggcgtact ccccggccgc ctcgacggcg gcagccatgc cgaggccggc cacacgcgcc
     2221 ccgcctcgcg catgcgagct atctcctccg gctccttcac cagtctgagt tgggagagaa
     2281 cgggcgtcgc atccttcggc tccatacccg cgggcttggc gtgggggtgc gtgagcatcc
     2341 gcagcatgga ctgagtgagg aaggagtact ccaagcccac gacgccactc tccaggccga
     2401 actcccggaa gtcgtgggcg atggcgtgga gcatccccac ctcgtcctca aaacccgcga
     2461 tgcggtcgaa gcgcgccagg acagagacat cagccacgtc ggtggaaagc acacccagag
     2521 cgactttgcc ctcctgggtg agcatggcgt aggcgcacag gcgaccgtcc ccagtgaggt
     2581 agaacatgtt cgagggtttg gcaaggagca acacatcaag ttcttgcgcc cgtatgagtt
     2641 cgccgcagcg cgcaacccgt ttctcgtatg cgaagcccgc catggggtca gcctctcctt
     2701 cttgtccgcc aattctgccg gtcgaccagg gtcgcccccg acgcggccac tcccgactag
     2761 agcaggcgcg tcgggtcagc ctggcgcggg gtcttcacca ccaggaagcg aaacgtctcg
     2821 tcgccctcgt tcagcagccg gtggggaatc ttggctgggc tctcgatgag catgtctttg
     2881 gatacgcctt cgctctcgtc gccgatctcc accgtgcctg agccctccag gacgtagaag
     2941 aaggcatcca ccggcgtgct gtgcaacttg agcgcctcac ctgggcggag ggtgatcatc
     3001 gacacttgca cgtgctcggt gctgtggaga ggacgggcgc tcactccgtg ggggttgacg
     3061 gtctcggggg cttcggacgc cgttacgatc ttcatctgct cctcctgtga ctcctcgggg
     3121 acgccgggtt gcgatgcgcc cgacgcaaac tcgggtccac aacaccggcc agcgtcgaca
     3181 cggttctcct ccttgcctcc acacaaaacg cgttctccaa gactatccgt tggtccccgt
     3241 cgccgaaact tcccgtgcgc atcaccgcct cccagcgacg tcgcgctgca gactggcccc
     3301 catccgcgca tgaggctctc ggtcagatgt tggactccgc tttcggagac gacgattcgg
     3361 aggatggcga cagcgccggc cacagtgcat tgatgcgggc cgtgacggct tcctggtcgg
     3421 ctcggccccc cgtcacgaga cgagcgagga gcggtccgat caggaggtcg atcatcacct
     3481 cgatatcgat gtcggttcgg acgcgcccca ccgactgtcc gcgctccaga atc
//
LOCUS       DASRMJ010000031        25716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_401251, whole genome shotgun sequence.
ACCESSION   DASRMJ010000031 DASRMJ010000000
VERSION     DASRMJ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 25716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25716
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401251"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFD74_00820"
     CDS             <1..631
                     /locus_tag="VFD74_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48127.1"
                     /translation="QAFYIGRLFFLVFFGEPRTSGEQKVVRESPWVMTLPLVVLGSAA
                     VFSGFAGARFLGRPLQNFLSIEGPAADPQGAPAWFVALAVVIGAAGVGGAWLAYGRSA
                     SRVGSSTGFDARLEAAMGQAYGVVERAYLVDELYSRVIVRPLVFAGRFLWSGVDGRVI
                     DRGVDGLARGVLGLGALVRRAQTGEVRDYLATMALGVVLIVALALGGAR"
     gene            628..2043
                     /locus_tag="VFD74_00825"
     CDS             628..2043
                     /locus_tag="VFD74_00825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZK48128.1"
                     /translation="MTDIVLVLPKVAIGAAALLALLLDSVTRGRARRQVILAVVVLLL
                     AAAATSAAGVGRTGTFLEGTLVVDRFSVFADVVLLVLAAVVVLAAMDGVGAGEDAGDF
                     YTLLFLSLAGASLLASAGRLIMVFLAVELSIIPTFALVAFRAEDRRSFEAALKYFLLS
                     VFASAVMLFGLSLIFGAEGSVALPLPPDTASSGLLLAGLGLVLAGFGFKLAAFPFHQW
                     LPDAFEVAHPEVAAFLAVGPKLAAVVALVRILEGLAAESGAWTSAIAAVAVLSMVWGN
                     LGAFRQKRIPRLLAYSAIAHAGYALIGLAGGGPRGVDGAVIYFAAYSAAAVGAFLVVG
                     VLARGGIGDDLVDLAGLGRKRPLVAAAMTLFMLSMVGIPLFAGFWGKFSVFLGAIQGG
                     VVWLAVVGVLNSALSFGYYGNVIRLMYLDGPTGADGGLSAHETGGWGLRVALATAAGF
                     TMLLGVAPALLFGSLGANSGL"
     gene            complement(1976..3229)
                     /locus_tag="VFD74_00830"
     CDS             complement(1976..3229)
                     /locus_tag="VFD74_00830"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ceramide glucosyltransferase"
                     /protein_id="HZK48129.1"
                     /translation="MITTLEILLLVSITFAAAQFAAAWRHLSRPGGPSSGCAPPRGNE
                     DWTPPVSILKPITGFEDGLRANLETFLHLDYPDYEIVFCLQDPEDPALPLLKELRAQH
                     PRRDVSIVVSDYSGGLNPKICNLIPGYDRARRDLVLVSDANVRVRPDYLREAVSHMRD
                     PHVGLVTHVVRGAKGTALGADLDNGYLNSFVVGAVALFDLVGISCVIGKSMLMRRRDL
                     ERLGGLEAVQDYLAEDFALSARFKRAGFKVVISPYPVDRVAMRGDVRSFVRRYTRWNT
                     MRRTIAGPAYLFEALANPSAIAVALVAAAVFGLGPDNPTIFIAGAAVGVKMALDAGTQ
                     ILFGVPVRAREVLLAPMRDLILFYVWAAGLCSRTISWRNTKLVVGRGSRLKVPDARPI
                     RQKLKDPSSLPGIRRVVRARRPAAS"
     gene            complement(3226..4584)
                     /locus_tag="VFD74_00835"
     CDS             complement(3226..4584)
                     /locus_tag="VFD74_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HZK48130.1"
                     /translation="MPSHPHPLLDPDTFLWGASTSAFQIEGAPAADWTTWKVKDAEDG
                     AVRVRGVDHRARQDADLGLLGEIGANAYRYSIEWSRVVPARGEWDAAEVDRQVRVARS
                     LRERGIEPVVTLHHFTSPAWLGQEGLDWTHEAFVSEFLRFATRMVEALAGDVRIWVTL
                     NEPNVHVGGGFLTGMTPPGKRGFRAAHLALTNMLRAHARLYEVIHTDSPGPTAVGIAH
                     NMLCFAPGRSDSRLDRWAARMADRIYNDTLIEAFRTGAVALRAIPVIGQVTVLPLRGH
                     LDFLGVNYYTRALVSFSPFDLRRYRYFWEDHPGRGLIETSWEVYPEGLSDVLQKAASL
                     GLPLVITENGAAEADDDRKVAFLREHVAQVARCRAEGMDVRGYFWWSLLDNYEWLRGL
                     EPRFGLYHVDFNTLERTPTRAATAYTRLVEAGIGRIGIDMNTLPPQPHSNRTPARSGA
                     IR"
     gene            4935..5132
                     /locus_tag="VFD74_00840"
     CDS             4935..5132
                     /locus_tag="VFD74_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48131.1"
                     /translation="MSIGWLSALAFPLLTSFFGCGMFGLLERDRRLGIVAVASLVAMA
                     ILVAASFWAGPPDLRAAIVRF"
     gene            complement(5351..6913)
                     /gene="gcvPB"
                     /locus_tag="VFD74_00845"
     CDS             complement(5351..6913)
                     /gene="gcvPB"
                     /locus_tag="VFD74_00845"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZK48132.1"
                     /translation="MTDDCRGSRLEPLTFDRSRPGHRGATVPTAGVPAAPIAHLLAGA
                     SLRTRPPALPELSEPEIVRHYTRLSHLNHSVDLGMYPLGSCTMKYNPKINDEVAQARG
                     FTDLHPYQTEDDAQGTLELMWELEQALLVVTGMCRATLQPAAGAHGELVGILMIRAFH
                     RDNGGASRDTVIVPDSAHGTNLATATMAGYKVVELKSSDRGLIDLDTLRSNLSEKTAA
                     LMLTNPNTLGLFEEDILEITRLTHEAGALVYYDGANLNAIMGKARPGDMGMDVVHMNL
                     HKTFSTPHGGGGPGAGPLAVCARLEPYLPAPLVSRRDPNEAPMRRADCREGCASGPDD
                     DGACCPRFGLDYDRPASIGRVRGFYGNVGVLERAYAYILSMGGDGLTQASEDAVMNAN
                     YLRTRLSGTYDIPHDRLCMHEFVASASRQKRDTGVAARDVAKRILDHGVHAPTIYFPL
                     IVPEALMIEPTETESLAGLDAFVDILVAIDREAHESPEVVTGAPHTTPIRRPDETRAA
                     RDPMLRWRSDTG"
     gene            complement(6903..8267)
                     /gene="gcvPA"
                     /locus_tag="VFD74_00850"
     CDS             complement(6903..8267)
                     /gene="gcvPA"
                     /locus_tag="VFD74_00850"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZK48133.1"
                     /translation="MSYLVHTDADRALMLRRIGAASVDDLFTPIPRELRTTALDLPPG
                     SPESEVAARVTSLAERNRTWGSRSFLGAGCYHIYVPAAVRSLTGRSEFATAYTPYQPE
                     VSQGTLQHIFEFQTTISELTELPVANASMYDGASALAEGAFLAARHTSRDGILVSAGV
                     HPEAREVVRTYASGPGLPVADLALDPSSGRTLVPDAARLEGAAAVLVQQPNFFGIIED
                     LEPLAEAAHAAGALLVVSQNLLAAGLLAPPGQVGADVAVGDVQPFGNPPNYGGPFAGF
                     MACADSYVRQLPGRLVGETLDADGRRCFTLTMQAREQHIRRAKATSNICSNQALCALA
                     ATIHLALLGPTGLRELGATCIARAHYLHQRLTELPGVSSAHDGPFFNEFAIRLPVDAA
                     LFAESLRELGVDPGVPLGRFDPAWTPLLLVAVTEINSPRDLDAYVDAAADALAHPKEA
                     VHDR"
     gene            complement(8267..9562)
                     /gene="gcvT"
                     /locus_tag="VFD74_00855"
     CDS             complement(8267..9562)
                     /gene="gcvT"
                     /locus_tag="VFD74_00855"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZK48134.1"
                     /translation="MTLTEPNGSTSMSQDRPEGGSRHLTLEDEHIALGARFAVFAGWR
                     MPTMYTSIIDEHLRVRTSAGLFDVSHMGRLLLRGRDALRTVQRLVASDISDLGPGQAR
                     YTVLLTTEGGILDDLIVYRREDGILLVVNAGNTAEDRDWIGEHLAGDTTLDDLTDITG
                     LFALQGPAALNVIETISSAGASSLAPFTFTAANVAGVEATVMRTGYTGEQGVELLTAR
                     TDAVRLWRALLEFDGGALVAPCGLGARDTLRLEAALLLHGRDIDRSTTPYEARLGRLV
                     DLDRKDGSDFAGRAALLRAAADGPTRLLVGLANQARTIPRSGDSIHVDGRRVGTVTSG
                     SLSPVLGHPIALGYVEPEAAQTGTEVSVIAGTKTTPARIVDRPFYRRGETPIPSRARR
                     APSSSHRPGSSAAWTGSSAAGTEAPTDRPGPPTAGPTEA"
     gene            9783..10346
                     /locus_tag="VFD74_00860"
     CDS             9783..10346
                     /locus_tag="VFD74_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48135.1"
                     /translation="MRKIIGHRWAIGAAALVFVFALGAISWAATTDTTSTTDGTATGP
                     AGDPGLDGIAPGSGMLGPEGFAPGRGFSAFGRMGRPGLRGGAVTEEMKAQMEARRAQV
                     EERRDAFIDLVGAKMSPTDQAALDSYLDTAKSQRAELEKAQEALHATNSEIRDLVGKY
                     FPSGDELGATDSTGGTGAAGTTANPGV"
     gene            10538..11332
                     /locus_tag="VFD74_00865"
     CDS             10538..11332
                     /locus_tag="VFD74_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK48136.1"
                     /translation="MGDNSSRSTEVYGEEAGVAARREGELGTIVVVEDDPSISELLEL
                     YLGAEGYLVIPTASAAAASGLVKSSAPALVILDLMLPDGSGLRVLKDIRVFSPVPVII
                     LTAKDADQDKILGLELGADDYMTKPFNPGELVARVRAVLRRAAPAGESGVQVLGGVEV
                     LPEERVVRVDGIRIELTPREFDLLHYLLLNRGIALSRERLLEEVWGYSFYGDARTVDV
                     HIRQVRKKIGRWAVVVQTVWGVGYRADASAQPDDGASETGEAEAED"
     gene            11365..12870
                     /locus_tag="VFD74_00870"
     CDS             11365..12870
                     /locus_tag="VFD74_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZK48137.1"
                     /translation="MTGPRSAGGSIRLRIAAALIGVAVLTLLMVSLLFYGFLGNYVLR
                     QKQEAMMAHAVEVANQVETLVDLGPLRSLATARGLAAVLRLDMQVLPSGAGITMFDGE
                     RVLAAAGPPRTQGAMAALLLPEARKLAENGAASTTFRIEGDQRLVVAAAPVDLGTGRP
                     GLIVVSLPTSDAVADRRGMFRILLVAGLVGVGFALVVGLGLGEWLSRPLRRLSRAAGT
                     MAAGSYAEPITGTYPSEVFDLASSLETMRREVRHSDESLRGFVASAAHELRTPLTSIQ
                     GFSQALLDGTADSPDQRRRSAAAVFRESTRLRRLVDALLILSRFDSREFRPTVAPVDP
                     MGLVGEEVDRLVEAGMAEAGRISLRATPAAAATRLSTDADMLRQVIGNLLRNAVQYGG
                     DDPIVVASHVAGDRFMLDFENGGEKLGEGDRRRVFERFYRAPSSLRHEGFGLGLPLVR
                     EICEVLGGEVDVTVPPADAAGGSEAVSPRTRFRVTLPLVWQAVVPPVAPEG"
     gene            12867..14381
                     /locus_tag="VFD74_00875"
     CDS             12867..14381
                     /locus_tag="VFD74_00875"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HZK48138.1"
                     /translation="MSDLVLMAGDDLVEVTIGGAVRVIDPPPPLEPLPDFAKSVRRAL
                     RAPMGLPQLRRLVGPGARVTVAFDDPCLPLPPPWVDPRRQVLEQVVDALSDAGVRDHD
                     LTLVCANGLHRRWRARELAPIVGPRLRRRFGDRIVCYDAEDAEANVSLGPTESGLLVE
                     VSRLVADADLVVYVGVPWTEMNGGHKSLACGLSTYACIAQHHRSAVQVSSPLMSPESS
                     AMHGALRDIGRHIGARVPVFQVETLVNNRLWAGPFRSLDLRRRRLSAGMRQARRAPAS
                     LRRLARRNMRGSYQPAGIWAGAVEPVHTALLERMAANRVAVEGQSDILVLGVPNMSPY
                     AVHADMNPLLTVNLGLGYSFQFGRGRPLVREGGIAVLVAPCSSGFDPVHHPSYERFWN
                     EVLSETRDAAVMEREWEPGFAVDADLVAAYRQGWAYHPAHPFFAWYWMARALRHLDTV
                     IVAGCEDPAVVEHMGFLPASNLERAVDMARGILGPDATVSVQSVPPVFTVDVSA"
     gene            14461..15885
                     /locus_tag="VFD74_00880"
     CDS             14461..15885
                     /locus_tag="VFD74_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK48139.1"
                     /translation="MRISFIEPAPPGFHIYSFVKQVRLGLPLLATMLRDAGHDVSVQV
                     ENLGELDWDRILGSDLVGISTITSTAIKAYRYAERARRAGIPVVMGGPHVTFEADEAL
                     EFCDYVVRGEGEQATFELLEVIGGSRDPSTVAGVSWKDPAGEVRHNQSRPLLGSTSHL
                     PTPDLSLIEHTEQISPTPLLTSRGCPHDCEFCSVIMMFGRRVRTDSEERILTAIRETR
                     PESIFFYDDNFVLSKRRTKALLARMVREGLQVPFSAQIRVDSVFKAGKTDHELLHLLA
                     SAGCYLVYLGLESANPETLRGFNKRQSVEDMAGGLAALHSYGIKSHGMFVLGSDSDTV
                     TSIRETVDFAIDTRISSAQFLVLTPLPGTRQTARFRAEGRIFTNNWTLFDGHHVVYWP
                     RNMSPWELQREMLAAHQRFYRIRRLPANPRHRLQGFLMSHGWERVPENMAYMRELRAF
                     CATHEPPPVVEVDFGEVLPAYGAS"
     gene            16003..16764
                     /locus_tag="VFD74_00885"
     CDS             16003..16764
                     /locus_tag="VFD74_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK48140.1"
                     /translation="MARVVALANQKGGVAKTTSTLNLGVALVELGKRVLAVDMDPQGN
                     LTMSQGLNPDDVQPSMYNVLIQGVDIGDVIHHMEIELAVANIDLAGAELALASVIGRE
                     RALSRALMAVHDSYDFILIDTPPSLGLLTINALTASDAIIVPVQCEYLSLRGLAQLER
                     TLELVRENLNPNVHIAGILPTLFDSRTLHGNEAVELLRSNFGDLVFETVVRKTIKFAE
                     APVQGSSVIKYAPRSDAAESYRSLAREVLNGKRKI"
     gene            16766..18058
                     /gene="ftsZ"
                     /locus_tag="VFD74_00890"
     CDS             16766..18058
                     /gene="ftsZ"
                     /locus_tag="VFD74_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZK48141.1"
                     /translation="MKNSGLGALFRSTGGHPEAHADGVAGGHGESVAGPLEEGGFGQS
                     SSKRQLIQPSRAFPAVIRIVGVGGAGTNAVNRMLEAGLEGVEFIAVNTDVQALQMCEA
                     DHKLRIGRELTKGLGGGSDPDVGSQAAHAAYDDIKALLKGSDMVFITAGEGGGTGTGA
                     SPVIAEIAKSVGALTIGVVSRPFAFEGNRRKVQASAGIEALRKFADTVIVVPNDKLLE
                     VVDRSTSMMDALRLADDVLRQGVQGICDLVTVPGLINLDLADVRTIMTSAGTAHMGIG
                     SARGEQRAQKAADQAINSALLETSIDGARGILLNISGGSDLSLFEVHEVAEVVNAAAD
                     PDVNIIFGAMVDEALGDLIRVTVVATGFDAAPRPKAAEGPSDAEATFHAGGEDPGEAL
                     TGERAASTSETTEGGTGEPGAEPEESLDVPPFLRGIDS"
     gene            complement(18141..18611)
                     /locus_tag="VFD74_00895"
     CDS             complement(18141..18611)
                     /locus_tag="VFD74_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK48142.1"
                     /translation="MQDTESTPARVRQADARDLDALAALLEEIMAHHGVTRPPEDAIR
                     DTLRAILASTSHLSLVAEVAGNIVGTCSLIFSISTWSAGPVCEVQDLIVTARHRGTGV
                     GRLLLEAARDAALDRGCVRLFLYAEPFNLGAHAFYRSLGMEEKTALYFERSLAR"
     gene            18832..19758
                     /locus_tag="VFD74_00900"
     CDS             18832..19758
                     /locus_tag="VFD74_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZK48143.1"
                     /translation="MLSPMLYLNGSIMPLADGRVSVEDRGFQLGDGVYEVLKIDNGRL
                     LWLEDHLERLDWSTGQVLLSGALDGHPLAEILPRLVSMSGLRQGTAYVQVTRGAAPRE
                     FALPSGVEPTVLAYVRPHEFPSDDEVCAGIAVHAVEDLRWARCDIKSTNLLPAVLAKH
                     AAGEAGAREALYVSADGVVREGGSSNAFVVLDGTLRTHPANNRILNGITRCHVLDLAR
                     ELGFPVLEQAVHVSDLPRASEIFIASTIRDVMPVVRVGGLDTGMLTPRGGVPGEMTIA
                     LAEAMRSLIARCVGVPAPPRRFPDASKGNSTP"
     gene            19755..20747
                     /locus_tag="VFD74_00905"
     CDS             19755..20747
                     /locus_tag="VFD74_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK48144.1"
                     /translation="MIAEKARARQRRALRLTISGVRGLVEMGRRLSMADRHVDLLTGA
                     PVPRRGAHYSADDDSTVSIYLNQIGVVPLLTAEEEVALAKRIESGDESARLHMIEANL
                     RLVVSIARRYVGRGLSFSDLVQEGNFGLMRATEKFDYRRGNKFSTYATWWIRQAVTRS
                     LADKGRTVRLPVHIVERLSAIARTKGELRQRLNREPRLEEVAEAVGLTAVELQALLDV
                     AESPLSLNASIDPEGDSTELGATVPDPNQVPSFDTVYQRLRARTVAEAITALPERERA
                     VLVFRYGLDDDDPWTLGEIGDHFGISRERVRQIESKAIERLRSNGLIRRLRESA"
     gene            complement(20722..21735)
                     /locus_tag="VFD74_00910"
     CDS             complement(20722..21735)
                     /locus_tag="VFD74_00910"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HZK48145.1"
                     /translation="MAGFYLLGRLVGRQRARVGAPTDRAGSALAGDGLPLIGAHVSTA
                     GSLEASIVRARHIGAEAIQVFPSNPRQWRPGPYAPEDLARYGLALRRARLPLFVHTIY
                     LINLAGPDANLRLKSAAALADALLFGALTGAAGVVTHVGSHRGEGFEAGLARTAAALT
                     QAYSLLETRLARASGAPASAAVVPPLLMESSAGGGDSMGRDPAELGRLVAAAPRRAGV
                     CLDTAHLYAAGYALDTAEGVERLHWELAAADCLDRVGLVHLNDSKTALGSRSDRHENL
                     WDGRYGRSGLRRLMSSAPLRTAPFVMEVPGADGHGTDRRNIRRARILRREAATLTPTR
                     EGA"
     gene            21734..22435
                     /locus_tag="VFD74_00915"
     CDS             21734..22435
                     /locus_tag="VFD74_00915"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZK48146.1"
                     /translation="MIPLRDNITTLRFAYVTYALIIINAMVFFYELSLGQRGYVDFIS
                     TWGYVPDLVTGTVQSGAFDPRILATLVTSVFLHGGWVHIGGNMLYLHIFGNNVEDAMG
                     RARFVFFYLLIGVAANFAQVVVSPHSLVPGIGASGAIAGVLGAYALLYPRAGVLVLIP
                     IFFFIRIITLPALAVLLFWFVIQVFQGSLTLAAGQAGAGGVAFWVHAAGFVLGAALIW
                     VFRNSELTGAKPTWI"
     gene            22426..22644
                     /locus_tag="VFD74_00920"
     CDS             22426..22644
                     /locus_tag="VFD74_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48147.1"
                     /translation="MDMKPENEIDDLKFLVGTACSRLINLLIDPRADAATLAAEATFL
                     AQRAERLAELLATEASAETPAEPRDAGS"
     gene            22704..23000
                     /locus_tag="VFD74_00925"
     CDS             22704..23000
                     /locus_tag="VFD74_00925"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48148.1"
                     /translation="MTDYDDSPDAMHGPARVQVEVGSRVIVGDIKTPDIAYKSRVSDV
                     VNDQDRRFLPLVDVELLDGPSGRVIGRSQFVLVRLDAVDVITPLREPGHRSPLD"
     gene            23003..24298
                     /locus_tag="VFD74_00930"
     CDS             23003..24298
                     /locus_tag="VFD74_00930"
                     /inference="COORDINATES: protein motif:HMM:NF023462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3524 domain-containing protein"
                     /protein_id="HZK48149.1"
                     /translation="MSVRPEAPPWLVAPSPGLRVWALEPYFGGSHRRFLEGLASHSRH
                     HYSLHTLPGRNWKWRMHGAALALARDSQGGRTIPAPGDADGARTIPAPGGAGGAALPQ
                     VLFASDMLDLADYKALVHPAVAATPTILYMHENQLTYPLPPGVERDLGYGLKNIISAL
                     VADRVLFNSAFHRGEFLGAVEALLDDLPDAVPHWAADEVAAKSDVLPLGCDLAWLDGH
                     RPSGGPGHSGSGRWGDEERGPLIVWNQRWEYDKAPGDLFRALSALRERGVGFRLAMAG
                     ANHGLPSAQFVEARGRLDSEIVQWGHVEDAADYASLLWEADIVVSTALHEFFGVAVVE
                     ALYCGCRPVLPHRLSYPEIVPKEAHGEALYGPGELVDALAEALERSTAARKSAGKSSR
                     IEWQRTWVSRYDWSAMASRYDAAVQLCWEGGLARGTKEG"
     gene            24298..25716
                     /locus_tag="VFD74_00935"
     CDS             24298..25716
                     /locus_tag="VFD74_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZK48150.1"
                     /translation="MERIAIDYTLCMEEALGPRKGLSRFLLEALRLPLEEARGRLAEK
                     RHAGGLAWMDLPRRDASDVLDFAERATGRYDDIVVLGIGGSALGTTALTSALLHPFHN
                     SIPAAARGGRPRVFVVDNVDPDEIGSLFTHLDLERTLVNVISKSGTTAETMAAYLVAR
                     GLLVERVGEGALRDHLAFTTDPHGGVLRKIGRQDGIPLFDVGAGVGGRFSVLSPVGLL
                     PAALAGIDVVGLLAGAAEMDQWIAEADVMSNPAYLFATLQFLQDTRFQRHLSVMMPYS
                     RALRDVAVWYRQLWAESLGKAVDRRGRTVHVGPTPIDALGATDQHSQVQLYTEGPDDK
                     VFTFLRVDEFSYDVVIPDLHAAEESLAYLGGRSMSDLLNAEQEATAWALAQRGRPSLT
                     ISVPRVDAGSVGQVLYLLEVAVSVAGELYDINAFDQPGVELGKQATYALMGRAGFEEL
                     AGRIRGTVGGDGSAAGVRYLVH"
BASE COUNT         3855 a         8254 c         9186 g         4421 t
ORIGIN      
        1 acaggccttc tacattggcc gtctcttctt cctcgtgttc ttcggagagc ctcgcacctc
       61 gggagaacag aaggtggtgc gggagtctcc ctgggtgatg actctgccgc ttgtcgtcct
      121 cgggtcggct gcggtgttct ccggcttcgc cggcgcgagg ttcttgggcc gacccttgca
      181 gaacttcctc tcgatagaag ggccggccgc cgatcctcag ggagcccccg cctggttcgt
      241 ggcgctcgcc gtcgtcatcg gtgccgccgg cgtcggcgga gcgtggctgg cgtacggacg
      301 ctccgcgagc cgagtcgggt cgagcacggg cttcgacgcg cggctggagg cggcgatggg
      361 ccaagcgtac ggtgtcgtcg agcgcgccta cctggttgac gagctgtatt ccagggtcat
      421 cgtgcgccca ctggtcttcg ccgggcggtt cctctggtcg ggtgtggatg ggcgggtcat
      481 cgatcgcggc gtcgacggtc tggcgcgggg cgtccttggg ctgggcgccc tggttcgacg
      541 agcgcagacc ggagaggtcc gggactacct ggccacgatg gccctggggg tcgtgctcat
      601 cgtggcgctg gctctggggg gtgcgcggtg acggacatcg tcctcgtgct tcccaaggtg
      661 gcgatcggcg cggccgccct gctagcgctg ctcctcgatt cggtcacccg gggacgcgcg
      721 cgccggcagg tgatactcgc ggtggtggtg ttgcttctcg ccgccgcggc gacgtcggcg
      781 gcgggcgttg ggcggacggg gacgttcctc gaggggacac tggtggtcga ccgcttctcc
      841 gtcttcgccg acgtagtcct cttggtcttg gctgccgtgg tcgtgctggc ggcgatggac
      901 ggagtcgggg cgggagagga cgcaggcgac ttctatacgc ttctgttcct gtccctggcc
      961 ggcgcctcgc tcctcgcgtc cgccggcaga ctcatcatgg tgttcctcgc cgtcgagctg
     1021 tctatcatcc ccaccttcgc cctcgtggcg ttccgtgctg aagatcggcg cagtttcgaa
     1081 gccgccctca agtacttcct gctcagcgtc ttcgcctcgg ctgtcatgct cttcggtctc
     1141 tcgttgatct tcggcgccga gggctcggtg gcccttcccc tgccgcccga cacggcttcg
     1201 tcgggactgc tcttggccgg gctcggtctc gtgctggcgg gcttcgggtt caagctcgca
     1261 gccttcccct tccaccagtg gttgcccgat gctttcgagg tcgcgcaccc ggaggtggcc
     1321 gctttcctcg ctgtcgggcc gaagctggcg gcggtcgtgg ccctcgttcg catcttggag
     1381 ggactggctg cggagagcgg tgcgtggacc tctgcgatcg cggctgtcgc ggtgctgagc
     1441 atggtgtggg gcaatctcgg cgcattccgc cagaagcgga ttccgcgctt gctggcgtac
     1501 tctgccatcg cacatgcggg gtacgccctg atcggcttgg cggggggcgg cccgcggggc
     1561 gtcgatggag ccgtgatcta cttcgccgcc tatagcgcgg cggcggtagg tgcgttcctg
     1621 gtcgtcgggg tcctcgcacg cggcggaatc ggggacgacc tcgtcgatct tgccggtctc
     1681 ggtcgaaagc gcccgctggt ggcggcggcg atgacgctct tcatgctgtc gatggtaggg
     1741 ataccgctgt tcgcgggttt ctgggggaag ttctcggtgt tcctgggtgc gatccagggc
     1801 ggcgtggtgt ggctggccgt tgtgggtgtg ctcaacagcg ccctgtcgtt cgggtactac
     1861 ggcaacgtga tccgcttgat gtatctggac ggtccgacag gcgccgacgg cggtctatcg
     1921 gctcacgaga ccggcggctg gggcctccgt gttgcccttg cgaccgccgc cgggttcacg
     1981 atgctgctgg gcgtcgcgcc cgcactactc ttcggatccc tgggagcgaa ctcgggtctt
     2041 tgagcttctg cctgatcggc cgcgcgtcgg gcaccttgag tctcgaaccc ctccctacga
     2101 ccaacttcgt gttgcgccac gagatcgtgc ggctgcagag accggcggcc cagacgtaga
     2161 agaggatgag atcgcgcatg ggggccagca gcacctcgcg ggcgcgcacc ggaacgccga
     2221 acaggatctg cgtgccggca tcgagggcca tcttcacccc cacggcggcg ccggcgatga
     2281 agatcgtggg attgtcgggt cccagcccga agacggccgc cgccaccagc gccacggcga
     2341 ttgcgctggg gttggccagc gcctcgaaca ggtaggccgg acccgcgatg gtgcgccgca
     2401 tggtgttcca cctggtatag cgtcgcacga agctgcgaac gtctccgcgc atcgcgaccc
     2461 ggtcgacggg atagggcgag atgaccacct tgaagcccgc tctcttgaag cgcgcggaaa
     2521 gggcgaagtc ctccgctagg tagtcttgca cggcctcgag cccgcccaac ctctccaggt
     2581 cgcgcctgcg catgagcatg gacttgccga tgacacagct tatccccacg agatcgaaca
     2641 gggcgaccgc ccctactacg aacgagttca ggtagccgtt gtcgaggtcg gcgcccaggg
     2701 cggtgccctt ggctcctcgc acgacgtggg tcaccaggcc cacgtggggg tcgcgcatgt
     2761 gcgagacggc ttcgcgcaga tagtccggtc gcacgcgcac gttcgcgtcg ctcaccagca
     2821 cgaggtcgcg tcgcgcacga tcgtatcccg ggatcaagtt gcagatcttc ggattgaggc
     2881 cgccggagta gtcgctcacg actatgctga cgtcgcgtcg aggatgctga gccctgagtt
     2941 ccttcaggag cggcagggcc gggtcttctg ggtcttgcag gcagaagact atctcgtagt
     3001 ccgggtagtc caggtggagg aaggtctcga gattggctcg aaggccatcc tcgaatcccg
     3061 tgatcggctt gaggatggaa accggaggag tccaatcctc gttgcccctc ggaggtgcgc
     3121 acccggagct gggaccaccg gggcggctca ggtgtcgcca cgccgcggcg aactgggcgg
     3181 cggcgaacgt aatcgagacg aggagaagga tctcgagcgt cgtgatcatc tgatggcgcc
     3241 ggaccgggcg ggtgttcgat tcgagtgcgg ctggggcggg agcgtgttca tgtcgatgcc
     3301 tattctacca atcccggcct cgaccagtcg tgtgtacgcg gtggcggcgc gagtgggcgt
     3361 gcgttcgagc gtgttgaagt ccacgtggta gaggccgaac ctcggttcca gtcccctgag
     3421 ccactcgtag ttgtcgagga gcgaccacca gaagtacccg cgcacatcca tcccttcggc
     3481 gcggcagcgc gcgacctgcg cgacgtgctc tcggaggaag gccaccttgc ggtcgtcatc
     3541 cgcttcggcg gcgccgttct cggtgatcac gagcgggagg cccagcgaag cagccttttg
     3601 cagcacgtcg gagagtccct ccggatacac ctcccagctc gtctcgatca atcccctgcc
     3661 cgggtggtcc tcccagaagt agcggtacct gcgaaggtcg aagggggaga agctgaccag
     3721 cgcgcgggtg tagtagttca cacccaggaa gtcgaggtgc ccgcggagcg ggaggacggt
     3781 cacctgtccg atgaccggga tggcacggag cgcgacggca ccagtgcgga acgcttcgat
     3841 cagggtgtca ttgtagatgc ggtcggccat tcgtgccgcc cagcggtcga gccgagagtc
     3901 ggaccgaccg ggggcgaagc acagcatgtt gtgcgcaata cccacggcgg tcggcccagg
     3961 cgaatccgtg tggatcacct cgtacagccg cgcgtgcgcc cgaagcatgt tggtgagcgc
     4021 gaggtgcgcg gcgcggaatc ctcgcttccc cgggggggtc atcccggtca agaagccgcc
     4081 gcccacgtgg acgtttggct cgttgagggt cacccagatg cggacgtcgc ccgccagggc
     4141 ctcgaccatc ctggtcgcga agcgcaggaa ctcggacacg aaggcttcgt gtgtccagtc
     4201 gagcccttcc tgccccagcc aggcggggga ggtgaagtga tggagggtga cgacgggctc
     4261 gatgcccctc tcgcgcaggg acctggccac gcgcacttgg cggtccactt cggccgcgtc
     4321 ccactcgccg cgtgcgggca ccactctgct ccactcgatg gagtagcggt aggcgtttgc
     4381 cccgatctcg ccgagcaggc cgaggtccgc gtcctggcgc gccctgtggt cgactccgcg
     4441 cacgcgcacc gccccgtctt cggcgtcctt gaccttccag gtggtccagt cggcggcggg
     4501 tgctccctcg atctggaacg ccgacgtgga ggcgccccac aggaacgtgt cggggtcgag
     4561 gagcggatgc gggtgggacg gcaagatgct cttctctcgg atcgatcagc acctcggcac
     4621 atgaaagcac ggtcgcgtgc cgtgggcaag cagacggccc acgcggacga agcggcccga
     4681 gcgcgggagt cggcgccggg acatcgggta ttcggccgga gcgagcaggc gacgggcctg
     4741 cccggcgcga gagacgcacc cgccactcga ccaggtcaaa ggctcgtccg ttgggggatc
     4801 gatagtccgt gctttcgcat cgggtttcgc cgttcggtca ctgtcacact gtttgtgcgt
     4861 cgatagcgca gatctccaag gagcggcgtt gacggccggg tcggactggt gaccacagca
     4921 tgacgggacg tgaaatgagt atcggttggc tgagcgcgtt ggcatttccg ctcttgacct
     4981 cgttcttcgg atgcgggatg ttcgggctgc tggagcgaga tcgacggctt ggtattgtcg
     5041 cggtcgcttc tctggtagcg atggccattc ttgtggcggc gagcttctgg gcgggcccgc
     5101 ccgatctgag agccgcgatc gtcaggttct gagggggata gcctccgcgc gcatcgcgta
     5161 gggccgccgc tgcgatcagc gcgagggatc caaggagcca gagtctgccg gtgagggcgg
     5221 cggccgccag cagaccggcg ctcgcggcgg cgacggccga gcgcacggcg ttgtgtagcc
     5281 tccaaaggcg cacggcttcc ccaccatgtc gaatccctgc accggccgga acttgcctgt
     5341 cgctacggct tcagccggtg tccgagcgcc accggagcat cgggtcgcgg gcggcccgcg
     5401 tctcgtccgg tctgcggatg ggggtggtgt gcggagcgcc cgtcacgacc tccggcgact
     5461 cgtgggcctc ccgatctatc gcgacgagga tatcgacgaa ggcgtccagg ccggcgaggc
     5521 tctcggtctc ggtcggctcg atcatgagtg cttcaggcac gatgagcggg aagtagatcg
     5581 tgggtgcgtg gacgccgtgg tcgagtatcc gcttggccac gtcccgggct gcgactccgg
     5641 tgtccctctt ctgtcgcgag gcggatgcca cgaactcgtg catgcagagg cgatcatgtg
     5701 ggatgtcgta ggtgccactg agccgggttc gcaggtagtt cgcgttcatg accgcgtcct
     5761 cggaggcctg cgtgaggccg tccccgccca tgctcaagat gtacgcgtag gcccgttcca
     5821 gtacgcccac attcccgtag aagccgcgga ctcggcctat gctcgcggga cggtcgtagt
     5881 cgagcccaaa gcgggggcag caggcgccgt cgtcgtcggg gccggaagcg catccctcgc
     5941 ggcagtcggc ccgtcgcatg ggggcttcgt tcggatcccg gcgggagacc agcggtgccg
     6001 ggaggtatgg ttccagccgg gcacacacgg ccagcggtcc ggcgccgggg ccgcctccgc
     6061 cgtgcggcgt ggagaaggtc ttgtgaaggt tcatgtgcac gacatccata cccatgtccc
     6121 ccgggcgtgc cttgcccatg atggcgttca ggttggcgcc gtcgtagtag accagagcgc
     6181 ccgcctcatg cgtcaggcgc gtgatctcca ggatgtcctc ttcgaagagt cccagcgtgt
     6241 tggggttcgt gagcatgagg gcggccgtct tctcggagag gttcgagcgg agggtgtcga
     6301 gatcgatcaa tccacgatcg gagctcttca actccacgac cttgtagccg gccatcgtcg
     6361 ccgtcgcgag gttggtgccg tgggcggagt ccggcacgat cacggtgtca cgggaggctc
     6421 cgccgttgtc gcggtggaac gcccggatca tgaggatgcc cacgagctct ccgtgcgctc
     6481 cggcggccgg ctgcagcgtg gcgcggcaca tgccggtcac gacgaggagg gcctgttcca
     6541 gctcccacat gagttcgagc gtcccctgag cgtcgtcttc cgtctggtag ggatgcaggt
     6601 cggtgaagcc gcgcgcctgt gccacctcgt cgttgatctt ggggttgtac ttcatcgtgc
     6661 acgagcccaa cgggtacatg cccagatcga cggagtggtt gaggtgggag agtcgcgtgt
     6721 agtgacgcac gatctcaggc tcggagagtt ccggaagcgc tggggggcgg gtgcgcaggg
     6781 aggccccggc cagaagatgg gcgatcggcg cggcgggcac cccggcggtc ggcaccgtcg
     6841 cgccccgatg gccggggcgc gacctgtcga aggtcagagg ctccaacctc gatcctcggc
     6901 aatcatcggt catggacggc ctccttcgga tgagccaggg catccgcggc tgcgtcgacg
     6961 taggcgtcga ggtcgcgcgg actgttgatc tcggtcacgg ccaccagcag taggggagtc
     7021 cacgcaggat cgaaccgccc gagggggaca cccgggtcga ctcccagctc gcgcagggac
     7081 tcggcgaaga gggcggcatc gaccgggagg cggatcgcga actcgttgaa gaacgggccg
     7141 tcgtgcgccg acgagacccc tggaagctca gtgaggcgtt ggtggaggta gtgagcgcgg
     7201 gcgatgcacg tggcgcccag ttcccgcagg ccggtcgggc cgagcagggc aagatggatt
     7261 gtcgcggcca gggcgcagag tgcctggttc gagcagatgt tcgacgtcgc cttggcccgc
     7321 cgtatgtgct gttcccgagc ctgcatggtc agggtgaagc agcggcgacc gtccgcgtcg
     7381 agggtctcgc ccaccaggcg ccccggcaac tgacgcacgt agctgtctgc acaggccatg
     7441 aagccggcga acgggcctcc gtagttgggc gggttgccga acggctgcac gtcgcccacg
     7501 gccacgtccg ctcccacctg tccgggcggc gcgagaaggc cggctgccag gaggttctgg
     7561 gacaccacta gcagggcgcc ggcggcatgc gccgcttccg cgagcggctc gaggtcctcg
     7621 atgatgccga agaagttcgg ttgctgcacg agcacggccg ccgcgccctc gaggcgcgcg
     7681 gcgtcgggga ccaaggtgcg accggaggac gggtcgagcg cgaggtcggc gacgggcaat
     7741 cctggtcccg aggcgtaggt gcgcaccacc tcgcgcgcct cggggtggac ccccgccgag
     7801 accaggatac cgtcgcggga cgtgtggcgc gccgccaaga acgccccttc cgcgagggcg
     7861 gaggcaccgt cgtacatgga ggcgttggcg accggcagtt cggtgagctc cgagatggtc
     7921 gtctggaact cgaagatgtg ctggagggtg ccctgactca cttccggctg gtagggagtg
     7981 tatgcggtgg cgaactcact gcgcccggtg agcgagcgca cggccgccgg cacgtagatg
     8041 tggtagcagc ccgcccccag gaaacttcgg gacccccagg ttcggttgcg ctcagctagg
     8101 gatgtgaccc gcgctgcgac ctcactctcg ggcgatcccg gaggaaggtc caatgccgtg
     8161 gtgcgcaact cgcggggtat gggcgtgaag aggtcgtcga ccgacgctgc gccgatcctc
     8221 ctgagcatca acgcgcggtc ggcgtcggtg tggacgaggt agctcatcat gcctccgtgg
     8281 gtccggcggt cggtggtccg ggccggtcgg tcggcgcttc ggtcccggct gcggacgaac
     8341 cggtccaggc agccgacgat ccgggccggt ggctcgatga gggtgcgcgc cgggcccggg
     8401 acgggatcgg tgtctcgccg cgccggtaga aggggcggtc gacgatccgc gccggcgtgg
     8461 tcttggtgcc ggcgatgacg gacacttcgg tcccggtctg agcggcctcc ggttccacat
     8521 atccgagggc gatcggatga ccgaggacgg ggctcagcga tccggacgtg accgtgccca
     8581 cacggcggcc atcgacgtgg atgctgtccc cggagcgcgg gatggtgcgg gcctggttcg
     8641 caaggcctac gagcagccga gtggggccgt ctgcggccgc gcgcagcagc gccgctcggc
     8701 cggcgaagtc agagccgtcc tttcggtcga ggtccaccag tcggcccagc cgcgcttcgt
     8761 acggcgtggt gcttcgatcg atgtcgcggc cgtggaggag aagggccgcc tcgaggcgca
     8821 gcgtgtcacg tgccccaagg ccgcagggag ccacgagtgc gccgccgtcg aactccaaga
     8881 gggcccgcca gagccggacg gcgtccgtgc gcgccgtgag gagttcgacg ccctgctcgc
     8941 ccgtgtagcc ggttcgcatg acggtggcct ccactcccgc cacattggcg gcggtgaagg
     9001 tgaagggagc cagactcgat gctcccgccg acgagatcgt ctcgatgacg ttcagggcgg
     9061 ccggaccttg gagtgcgaag aggcctgtga tgtcggtgag gtcgtcgaga gtcgtgtcgc
     9121 ccgccaggtg ctcgccgatc caatcccggt cctccgccgt gttgcccgcg ttgacgacga
     9181 gcaggatgcc gtcttcgcgt cgatagacga tgaggtcgtc cagaatgccg ccctcggtgg
     9241 tgagcagcac cgtataccgc gcttgccccg gcccgaggtc ggagatgtca ctggcgacga
     9301 ggcgctgcac cgtgcgaagg gcgtcacggc cccgcaggag aagacgcccc atgtgcgaca
     9361 cgtcgaacag ccccgcggag gtgcgcacgc ggaggtgctc gtcgatgatg gaggtgtaca
     9421 tggtgggcat gcgccacccg gcgaagacgg cgaaccgggc gcccaaggcg atatgttcgt
     9481 cctcgagggt taagtggcgc gatccaccct cgggtcggtc ttgcgacatg gatgtggagc
     9541 cgttcggctc ggtaagggtc acggatcctc ctcgttcaac ggtagtgggc ctaccccacc
     9601 cgcctcacgt ctacgcatcg gacaccgggg aaaccgctgc cctccgccgc gcgcacggtg
     9661 ccggcccgcg tcgctcttct ccatatgctg gcccttccct aacagtctca tcatcctcga
     9721 cccgcacagt agagagcgtc agagatgtga cgaccccccc ggaaggggga ggaaggcgtg
     9781 agatgaggaa gatcataggc catcgctggg cgatcggagc ggccgcgctc gtgttcgtgt
     9841 tcgcactcgg cgcgatctcg tgggcggcca cgacggatac gacgtcgacc acggacggga
     9901 cggcgacggg cccggcaggc gacccggggc tggacggtat cgcccctggg tccgggatgt
     9961 tgggtcctga agggttcgcg cccggccgcg ggttctcggc gttcggacgc atgggccggc
    10021 cggggctgcg aggcggtgcg gtgaccgagg agatgaaggc tcagatggaa gcgcgccggg
    10081 cgcaggtgga ggagcgacgc gatgcgttca tcgacctcgt cggtgcgaag atgtccccca
    10141 ccgaccaggc ggcactcgac tcgtatctgg acacggcgaa gtcacagagg gccgagctgg
    10201 agaaggcgca ggaggcattg cacgccacca actccgagat ccgagacctg gtggggaagt
    10261 acttcccctc gggtgacgaa ctcggcgcga ccgactcgac cggtgggacc ggcgccgccg
    10321 gcaccaccgc gaacccaggc gtgtagcggg cgatcacgcg cggggcgtgc gggttgtgcg
    10381 gccggccgcg aggcatcgcg gccggccgcc tgcgcgtccc gcggaagctc gtccgcgcgt
    10441 ccggcgcgaa gggatccgga tacggacatg gacatgcgtc cccggctgcc tctaagatgg
    10501 tcgcgggtgc tgaacagatg tggcggggag ccctgtcgtg ggcgacaact cctcgagatc
    10561 cacagaagtg tacggcgagg aggctggtgt ggcggcgcgg cgcgagggtg aactcggcac
    10621 gattgtggtc gtcgaggatg acccatccat ctcagagctc ctagagctct acctgggggc
    10681 cgagggctac ttggtgatcc ccacggccag cgcggcggcg gcctcgggac tcgtgaagtc
    10741 cagcgcgccg gccctcgtta tcctcgacct catgcttcct gacggctcgg gtctccgagt
    10801 gctgaaggac atccgcgtgt tctcgccggt gccggtgatc atcctcacgg cgaaggacgc
    10861 ggaccaggac aagatcctgg gcttggagct gggggccgac gattacatga cgaagccctt
    10921 caaccccggc gaactggtgg ccagggtcag ggcggtcctg cgtcgggcgg cccccgccgg
    10981 cgagtccggg gtccaggtgc tcggtggggt ggaggtgctc cccgaggagc gggtggtccg
    11041 cgtggatggg atccggatcg agctcactcc ccgcgagttc gaccttcttc actacctcct
    11101 cctgaatcgg ggtatcgccc tctctcgcga gcggttgctc gaggaggtgt ggggctactc
    11161 cttctatgga gacgctcgga ccgtggacgt gcacatccga caggtgcgca agaagatcgg
    11221 ccggtgggcg gtggtagtcc agacggtgtg gggcgtcggc taccgcgccg acgcgtcggc
    11281 gcaacctgac gacggggcgt ccgagacggg cgaggcggag gcggaagact gatgtcggcg
    11341 tcgggccctg gggcttcaga ctcgatgacc ggaccgcgtt cagcgggagg gtcgatccgt
    11401 ctgcgcatcg cagcggcctt gatcggtgtc gccgtactca ctttgctcat ggtgagcctg
    11461 ctcttctacg gcttcctggg caactacgtc ctccggcaga agcaggaagc gatgatggcg
    11521 cacgcggtgg aggtggccaa ccaggtggag acgctcgtcg atctggggcc tctgcgatcg
    11581 ctcgccaccg cgcgcgggtt ggccgcagtc ctaaggctcg atatgcaggt gctgcccagc
    11641 ggcgccggca tcacgatgtt cgacggcgag cgggtgctgg ccgccgccgg gccaccccgc
    11701 acgcaagggg ctatggcggc gctgctgctc ccggaggcgc ggaaactcgc ggagaacggg
    11761 gccgcgtcca ccaccttcag gatcgaaggc gaccagcgcc tggtggtggc agccgcgccc
    11821 gtcgatctgg ggaccgggcg cccgggattg atcgtggtgt cgctgcccac ctctgacgcc
    11881 gtcgccgatc ggcgcggcat gttccgcatc ctcctggtgg ccggactcgt gggcgtgggc
    11941 ttcgctttgg tcgtgggctt gggtctggga gagtggttga gccggccgct gaggcgtctc
    12001 tcgcgagcgg cggggaccat ggccgccggt tcctacgccg agcccatcac gggcacctac
    12061 ccgagtgagg tgttcgacct ggcgtcgagc ctggagacca tgcgtcgcga ggtgcgccac
    12121 agcgacgaat cgcttcgcgg cttcgtggcc tccgcggctc acgagttgcg cactccgctc
    12181 acgtccatcc agggcttctc gcaggcgctg ctcgacggca ccgccgactc tcccgaccag
    12241 cgtcggcggt cggccgccgc ggtctttcgc gagtcgactc gactgcggcg gctggtggac
    12301 gccctcttga tcttgtcgcg gttcgactcg cgtgagttcc ggccgacggt ggcgcccgtg
    12361 gacccgatgg gcctcgtggg cgaggaggtc gaccggctgg tcgaggccgg catggccgag
    12421 gcgggccgca tcagtctcag ggccaccccg gcggcggctg cgacgcgtct gagcaccgac
    12481 gcggacatgc ttcgtcaggt catcggcaac ctgctccgca acgccgtgca gtacggagga
    12541 gacgacccca tcgtcgtggc ttcgcatgtg gcgggtgaca ggttcatgct cgatttcgag
    12601 aacggcgggg agaagctagg cgagggagat cgccgcaggg tgttcgagcg cttctaccgt
    12661 gcgccttcga gtctgcggca cgaaggcttc ggactcggtc tgccgctggt gcgcgagata
    12721 tgcgaggtcc tcggcggaga ggtggacgtg accgtgcccc ccgccgacgc ggccgggggc
    12781 tctgaagcag tgtccccgcg caccaggttc cgcgtcacgc tgccgttggt gtggcaggcg
    12841 gtcgtcccgc cggtggcccc cgaggggtga gcgatctcgt actcatggcg ggcgacgatc
    12901 tcgtcgaggt gacgatcggg ggcgcggtcc gggtgatcga tccacctccg ccactggagc
    12961 cgcttcccga tttcgcgaag tcggtgcgcc gggccctgcg ggcgccgatg ggcctgcctc
    13021 aattgcgtcg cctggttggc cccggggcgc gtgtgaccgt ggccttcgac gatccctgcc
    13081 ttccactgcc gccgccgtgg gtcgatcctc gaaggcaggt gctggagcag gtggtcgacg
    13141 cgctctccga cgcgggagtc cgcgatcacg acctcacctt ggtgtgcgcg aacgggctgc
    13201 accgccggtg gcgggcgcgg gagttggcgc ccatcgtcgg cccccggctg cggcgcaggt
    13261 tcggcgaccg gatcgtgtgc tacgacgccg aagatgccga ggccaacgtc tcgctcggac
    13321 ccaccgagtc gggcctgctg gtggaggtga gcagactggt ggccgacgcc gacctggtgg
    13381 tgtacgtggg agtcccgtgg accgagatga acggcggcca caagtcgctg gcgtgcggcc
    13441 tctccaccta cgcctgcatc gctcagcacc atcgctccgc ggtgcaggtg tccagcccac
    13501 tcatgtctcc ggagtcctcc gcgatgcacg gtgccttgcg cgacatcggg cgccacatcg
    13561 gcgcacgtgt gcctgtcttc caggtggaga cgctggtgaa caacagactg tgggcgggtc
    13621 cgttccggtc gctcgacctg cgtcggcgcc gcctctccgc agggatgcgg caggcccgca
    13681 gagcccccgc ctccctgcgt cgcctcgcgc ggcggaacat gcgcggttcc tatcaaccgg
    13741 ccgggatctg ggcgggcgcc gtggaaccgg tgcacacggc cctgctggag cggatggcgg
    13801 cgaaccgcgt cgccgtggag ggtcagtccg acatactcgt gttgggtgtg cccaacatga
    13861 gtccctacgc ggtgcacgcc gacatgaacc ccttgttgac cgtgaacctg ggtctggggt
    13921 actcgttcca gttcggccgg ggaaggccac tcgtgcgtga gggcgggatc gcggtgctgg
    13981 tggctccgtg ttcctccgga ttcgatccgg tccaccaccc ctcctacgag cgcttctgga
    14041 acgaggtgct ctccgagaca cgcgacgccg ccgtcatgga acgtgaatgg gagccgggct
    14101 tcgccgtcga cgcggatctg gtcgccgcct accgccaggg gtgggcctac caccccgcgc
    14161 atccgttctt cgcctggtat tggatggcgc gggccctccg gcatctggat acggtgatcg
    14221 tggcgggttg cgaggatccc gcggtcgtgg agcacatggg gttcctgccc gcctcaaacc
    14281 tggaacgggc ggtcgacatg gcccgcggga tcctgggacc ggacgccacc gtgtcggtgc
    14341 agtcggtgcc tccggtcttc acggtcgacg tctccgcgta ggagggttgc ccccgttccg
    14401 gcggcggcgt agagtgttgc gacgaccatg ggccgcgcgg ccccacatcc gggagtctag
    14461 gtgcggataa gcttcatcga gccggcgccc ccagggttcc acatctacag ctttgtgaag
    14521 caggtgcgtc ttgggctgcc ccttctcgcc accatgcttc gcgacgcggg acacgacgtc
    14581 agcgtccagg tggagaacct gggggagttg gactgggatc ggatcctggg ttccgacctc
    14641 gtgggcatct cgaccatcac ctccaccgcg atcaaggcct accggtacgc ggagcgcgct
    14701 cgtcgggcgg gtatccctgt ggtcatgggc ggaccccacg tgacgttcga ggccgatgag
    14761 gccctcgagt tctgtgacta cgtggtgagg ggagagggcg aacaggccac gttcgaactg
    14821 ctcgaggtga tcggtgggtc ccgcgacccg agcacggtgg ccggagtctc ctggaaggac
    14881 cccgccggcg aggtgcgaca caaccaatct cggcctctct tgggatccac ttcgcacctg
    14941 cccacgcccg acctctcgct gatcgagcac accgaacaga tcagcccgac cccgttgctc
    15001 acgtcgcggg ggtgcccgca cgactgcgag ttctgctcgg tgatcatgat gttcgggcgc
    15061 cgggtgcgca ccgactccga ggagaggatc ctgacggcga tccgcgagac gcggccggag
    15121 agcatcttct tctacgacga caatttcgtg ctctccaagc gtcggacgaa ggcgctgttg
    15181 gctcgcatgg tccgcgaggg gctgcaggtg cccttctcgg ctcagatccg cgtcgattcc
    15241 gtcttcaagg ccggcaagac cgaccacgaa ctgctgcacc ttctcgcaag cgcgggctgc
    15301 tacctcgtgt accttggcct ggagtcggcg aacccggaga cactgcgggg gttcaataag
    15361 cggcagagcg tcgaggacat ggccgggggg ttggcggcgc tccactccta tgggatcaag
    15421 agccacggca tgttcgtgct gggatcggac tcagacacgg tcacttcgat ccgggagact
    15481 gtggacttcg ccatcgatac ccgcatcagt tcagcgcagt tcttggtgtt gacccccctg
    15541 ccggggacgc gacagaccgc taggttccgg gcagagggcc gcatcttcac caacaactgg
    15601 accctgttcg acggtcacca cgtggtctac tggccgcgga acatgagtcc gtgggagctg
    15661 caacgggaga tgttggcggc acatcagcgc ttctaccgca tccggcggtt gccagcgaat
    15721 ccgcgtcacc gcctgcaggg attcctcatg agccacgggt gggagagggt gccggagaac
    15781 atggcctaca tgagggagtt gcgggccttc tgcgccacgc acgagccccc gcccgtcgtc
    15841 gaagtggatt tcggagaggt cctccctgcc tacggtgcgt cctagatccg cattctctcg
    15901 cgcttcgcca aggaatcccg cggtttcccc cgaaagcgta gctaccgtat aatcacatgt
    15961 gttcttgagt gccactggca gcacgaagaa ggtaggtatc acttggctcg ggtcgtagcc
    16021 cttgcgaacc agaaaggcgg ggtggccaag accacctcca ccctcaatct cggagtagcc
    16081 ctggtagagc tcggaaagcg ggttctggcg gtggacatgg atccgcaggg caacttgacc
    16141 atgagccagg gcctcaaccc cgacgatgtg caaccgagta tgtacaacgt gctcatccaa
    16201 ggtgtcgata tcggcgacgt gatccaccac atggagatcg aactcgccgt ggcgaacatc
    16261 gacctggccg gcgcggagtt ggccttggcc tcggtcatcg ggcgcgagcg cgccctgagc
    16321 cgggcgttga tggccgtgca cgactcctac gacttcatcc ttatcgacac gccgccctcg
    16381 ctcggtcttc tgactatcaa cgccctgacc gcgtccgacg cgatcatcgt gcccgtgcag
    16441 tgcgagtatc tctcgctccg cggtctggcg cagctggagc gtacgctgga gttggtgcgg
    16501 gagaacttga accccaacgt tcatatagcc gggatcctcc ccacgctgtt cgattcgcgc
    16561 acactccacg gcaacgaggc cgtcgaactg ctacgctcga acttcggcga tctggtgttc
    16621 gaaacggttg tccgcaagac gatcaagttc gctgaggctc ccgtgcaggg gtcctcggtc
    16681 ataaagtacg ctccgcgaag cgacgccgcc gaatcctacc gctcgttggc cagagaggtg
    16741 ctcaatggaa aaaggaagat ctagcatgaa gaacagcggc ctcggcgcgc tcttccgttc
    16801 cacgggtgga caccccgagg cgcacgccga cggcgtcgcc ggcggccacg gcgagtctgt
    16861 cgcagggccg ttggaggagg gcgggttcgg acaatcgtcg agcaagcgtc aactcatcca
    16921 gccctcgcgc gccttcccgg ccgtcatccg catcgtcggc gtgggcggcg cgggcacgaa
    16981 cgccgtcaac cgcatgctcg aagccggcct cgagggcgtg gagttcatcg ccgtgaacac
    17041 cgacgtccag gccctgcaga tgtgcgaggc cgaccacaag ctccgcatcg gtcgggagtt
    17101 gaccaagggc ctcggtggcg ggtccgaccc ggacgtgggc agccaggcgg cccatgcggc
    17161 atacgacgat atcaaggcgc tgctcaaggg cagcgacatg gtgttcatca ccgccggcga
    17221 gggtggcggc acgggcacgg gggcctcgcc cgtcattgcc gagatcgcca agagcgtcgg
    17281 cgccctcact atcggcgtgg tgtcgcgtcc tttcgctttc gaggggaacc ggcgcaaggt
    17341 ccaggcgtcc gcgggtatcg aagctctgcg caagtttgcc gacaccgtca tcgtggtgcc
    17401 caacgacaag ctactcgaag tggtggatcg gtccacctcg atgatggacg cccttcgcct
    17461 cgccgatgac gtcctgcgtc agggcgtaca ggggatctgc gacctcgtga ccgtgcccgg
    17521 cctgatcaac ctcgaccttg ccgacgtgcg caccatcatg acctcggccg gcaccgcgca
    17581 catgggcatc ggctcggcgc ggggagagca acgggcacag aaggccgctg accaggcgat
    17641 caactcggct ctactcgaga cctcgatcga cggagcccgc ggcatcctct tgaacatcag
    17701 cggcggctcg gatctctcgc tcttcgaggt gcacgaggtg gccgaggtgg tcaacgcagc
    17761 cgccgatccc gatgtcaaca tcatcttcgg ggccatggtc gacgaggcgc tgggcgacct
    17821 catccgcgtc acggtggtgg ccaccggttt cgacgcggcc ccccggccca aggctgcgga
    17881 aggtccatcc gacgcggaag cgaccttcca cgccggcggc gaggaccccg gggaggcctt
    17941 gacgggcgag cgagcggcat cgacgtcaga gacgaccgaa ggtgggaccg gcgagcccgg
    18001 cgcggagccg gaggagagcc tcgacgtgcc cccgttcttg cgcggtatcg actcgtaggg
    18061 ctgcagtctt cgagtggggg ttggagcgga gttagcgcgc ctgctccgag tcggcgtgcg
    18121 ggcaccgtcc gcgtgaagtc ctaccgcgcg agactgcgct cgaagtagag ggccgtcttc
    18181 tcttccattc ccagggaacg gtagaaggcg tgggccccta ggttgaaggg ttctgcgtat
    18241 aggaacaggc gcacgcatcc gcgatccagg gctgcgtcgc gcgcggcctc caggagcagc
    18301 cgtcccactc ccgtcccgcg gtggcgggcg gtcactatga ggtcttggac ctcgcacacc
    18361 ggccccgccg accacgtgct gatcgagaag atcaggctgc atgtccccac gatgttcccg
    18421 gcgacctcgg ccaccaggga gaggtgggag gtcgaagcca ggatggcccg caaggtgtcg
    18481 cggatggcgt cctctggtgg cctcgtcacc ccgtgatgtg ccatgatctc ttccagaaga
    18541 gcggccagag cgtccaggtc gcgggcgtca gcttggcgga ctcgggcggg tgtggattcg
    18601 gtatcctgca tgtgtagaga ttagcaggac gatgcttgtc atgcggggtt cgggcgaccg
    18661 cggaacgcgc gacgggttcg gcgcgggccg ggaagaccgg gccgcgatgc ggaaagctac
    18721 gggaaagcta ggggaaaggt ggggcctgca gtggcgcgag cggactcaga aggtgggctc
    18781 ggcggcggag atcggggtgc cgggacctcg tccgcgggtg acggatcacc gatgctctca
    18841 cccatgctct acttgaacgg cagcatcatg ccgctggcgg acggccgggt gagtgtggaa
    18901 gaccggggat tccagctcgg ggacggggtc tacgaggtgc tgaagatcga caacggtcgg
    18961 cttctgtggc tggaagatca cctggagcgc ctcgactgga gcaccgggca ggtgctcctt
    19021 tcaggagctc ttgacggtca cccactcgcc gagatcctgc cgcggctggt gagcatgtcc
    19081 ggtctgcgcc agggaaccgc ctacgtgcag gtgacccgcg gcgccgcgcc cagggagttc
    19141 gcgctccctt cgggcgtgga gccgactgtt ctagcctatg ttcggcccca cgagttccct
    19201 tcagacgacg aggtgtgcgc cggcatcgcg gtgcatgcgg tcgaagacct gcgctgggcg
    19261 cggtgcgaca tcaagtcgac caacctgcta ccggccgtgc tcgcgaagca tgcggccggc
    19321 gaggccggcg cgcgggaagc gctgtacgtg agcgccgacg gagtggtgcg agagggtggg
    19381 tcttcgaacg cgttcgtcgt tctcgacggc acgctgcgca cgcacccggc gaacaaccgc
    19441 atcctgaacg gcatcacgcg ttgccacgtg ctcgatctgg ctcgagaatt ggggtttcct
    19501 gtgttggagc aagcggtgca cgtctccgac cttccgcgcg cctcggagat cttcatcgca
    19561 tcgaccattc gcgatgtgat gccggtggtg cgtgtagggg ggctggacac cgggatgctg
    19621 acgccccgcg gaggagtgcc cggggagatg accatagcct tggccgaggc gatgcgctct
    19681 ctgatcgccc gatgcgtggg cgttccggct ccgccgcgtc ggtttccgga tgcctcaaag
    19741 gggaatagta cgccgtgata gcagagaaag cccgcgcccg gcaacgtagg gccctgcgcc
    19801 tgaccataag cggggtccgt gggctggtcg agatggggag gcggctgagc atggctgatc
    19861 ggcacgtgga tcttttgacg ggcgccccgg tgcctcggcg aggggcgcac tacagtgcgg
    19921 acgacgattc cacggtcagt atctacctga accagatcgg tgttgtgccc ttgcttaccg
    19981 ccgaagagga agtggccctc gccaagcgga tcgagtcggg ggacgagtcg gcgcggcttc
    20041 acatgatcga agccaacctc cggctggtgg tgtcgatcgc ccggcgctac gtggggaggg
    20101 gattgagctt ctctgacctc gtgcaggaag gcaacttcgg ccttatgcgt gccacggaga
    20161 agttcgacta ccgccgcggc aacaagttct caacgtatgc cacctggtgg atccgtcagg
    20221 cggtgacccg atcgctcgcc gacaaggggc gcacggtgcg gctgccggtg cacatcgtgg
    20281 aacggctctc cgcgatcgcg cgcacgaagg gcgagctgcg gcagcgcctg aacagggagc
    20341 ctcgtctcga ggaggtagcc gaggcggtgg gcctcaccgc ggtggaactc caggcgctcc
    20401 tcgacgtcgc cgagagccct ctcagcttga acgcctcgat cgatcccgag ggcgattcga
    20461 cggaactggg cgcgacggtg ccggatccca accaggtgcc gtctttcgac accgtctacc
    20521 agaggctgcg cgcgcgcacc gtcgcggagg ccatcacggc gctgcccgag agggagcgcg
    20581 cggtcctcgt gttcagatac ggactcgacg acgacgaccc ctggaccctg ggggagatcg
    20641 gggaccattt cggcatcagc cgggagcggg tccgtcagat cgagagcaag gccatcgaac
    20701 gcctgcgctc caatgggctg atcaggcgcc ttcgcgagtc ggcgtgagtg tcgcggcctc
    20761 ccgcctgagg atgcgtgccc tgcggatgtt gcgccggtcg gtgccgtgcc cgtcggctcc
    20821 cggcacctcc atcacgaagg gcgccgtgcg gaggggtgca ctgctcatca ggcggcggag
    20881 gcccgaccgg ccgtagcgcc cgtcccagag gttctcgtga cggtcgctgc ggcttccgag
    20941 cgccgtcttc gagtcgttga ggtgcaccag gccgacccgg tccagacagt ccgccgcggc
    21001 cagctcccaa tgcagtcgct ccaccccctc agcggtatcc agcgcgtatc cggccgcgta
    21061 gaggtgggcc gtgtccaggc agacgcccgc cctgcggggg gcggccgcga ccagacgtcc
    21121 gagttcggcg gggtcccggc ccatcgagtc gccgcctccc gcgctcgact ccatgagcag
    21181 tggggggacg acggccgcgg aggctggcgc gcccgacgcg cgcgccagcc gggtctcgag
    21241 cagcgagtag gcttgggtga gggcggcggc cgtgcgggcc agccccgcct cgaacccctc
    21301 tccacgatgc gaacccacgt gggtcacaac gccggccgcg cccgtgaggg cgccgaagag
    21361 cagggcgtcg gccaatgcgg cggccgactt caggcgcagg ttggcgtcgg ggccggccag
    21421 gttgatcagg tagatggtgt gcacgaagag cggtaggcgc gcgcgacgca aggccagacc
    21481 gtagcgggcc aggtcttcgg gcgcgtatgg accgggacgc cactgcctgg ggttgctggg
    21541 gaacacctgg atggcctcgg caccgatgtg acgtgcccgg acgatcgatg cctcgagcga
    21601 acctgcggtc gatacgtgcg ctccgatcag cggcagtccg tcgccggcga gggcgctgcc
    21661 ggccctgtcg gtcggcgcgc cgacccgtgc tctctgtctg cccacgagcc tccccagaag
    21721 gtagaatccc gccatgatac cgcttcgcga caacatcacg acgcttcgct tcgcctacgt
    21781 cacctacgcg ttgatcatca tcaacgccat ggtcttcttc tacgagctgt cccttggcca
    21841 gcgcggctac gtcgatttca tcagcacgtg gggttacgtg cccgacctcg tcaccggcac
    21901 ggtgcagtcc ggagcgttcg atccgcggat cctcgccacc ctggtcacct cggtcttcct
    21961 gcacggcggg tgggtgcaca taggaggcaa catgctctat ctccacatct tcggcaacaa
    22021 cgtcgaagat gcgatgggtc gggcgcggtt cgtgttcttc tatctgctga tcggggtcgc
    22081 cgcgaacttc gcgcaggtgg tcgtgtctcc ccactcgctg gtgcccggca ttggggcgag
    22141 cggcgccatc gcgggcgtac tcggggcata tgcgctgctg tatcctcggg cgggagtcct
    22201 cgtcttgatc cccatcttct tcttcataag gatcatcacc ctgccggccc tggcggtgct
    22261 gctgttctgg ttcgtgatcc aggtgttcca ggggtccttg accctggcgg caggacaggc
    22321 gggtgccggc ggcgtggcct tctgggtgca cgcggccggg ttcgtcttgg gggccgccct
    22381 catctgggtg ttccgcaaca gcgaactcac gggagcgaag ccaacgtgga tatgaagccc
    22441 gagaacgaga tcgacgacct caagttcctc gtcgggaccg cctgcagtcg cctcatcaat
    22501 ctcctgatcg atccgcgggc cgatgcggcc accctggcgg ctgaggccac cttcttggcg
    22561 cagcgcgccg agcgcctggc cgaactactt gcgaccgagg catccgccga gacgcccgcc
    22621 gagccccgtg atgccggctc gtagcggcgg gcggctggtc atacctgctt cccttgcggg
    22681 gggccagccg atagaatcgg gccatgacgg actacgacga ctcaccggat gcgatgcatg
    22741 gacccgcgcg ggtgcaggtg gaggtggggt cgcgggtcat cgtcggcgac atcaagaccc
    22801 cggacatcgc gtacaagtcg cgggtgagtg acgtggtcaa cgaccaggac cggcgtttcc
    22861 tgccgctggt agacgtggag ttgctcgacg gtccctcggg acgggtgatc ggaaggtcgc
    22921 agttcgtcct ggtgcgtctc gacgccgtcg acgtgatcac gcctctgcgc gaaccgggcc
    22981 accgctcacc tctggactga tcgtgtccgt gcgccccgag gcgccaccct ggctcgtggc
    23041 gccgtcgccc ggtctccggg tatgggccct cgaaccctac ttcggcggat cgcaccgacg
    23101 cttcctcgaa ggcctggcct cgcactcgag gcaccactac tcgctgcaca cgttgcccgg
    23161 tcgcaactgg aagtggcgca tgcacggggc ggccctggct ctggctcgcg attcgcaggg
    23221 cgggaggacc atcccggcac ctggcgatgc ggacggcgcg aggaccatcc cggcacctgg
    23281 cggtgcgggc ggcgcggcgc tcccgcaggt gctcttcgcc tccgacatgc tcgacctggc
    23341 cgactacaag gccttggtgc atcccgccgt cgctgcgacc cctaccatcc tctacatgca
    23401 cgagaaccaa ctcacctatc ctctgccgcc gggcgtggaa cgcgacctgg gatacggtct
    23461 caagaacatc atcagcgcgc tcgtggccga ccgagtgctc ttcaattcgg ccttccaccg
    23521 gggagagttc ctcggtgccg tggaggctct gctcgacgat cttccggacg ccgtgcccca
    23581 ctgggcggcg gacgaggtgg cggcgaagtc cgacgtgctg cccctcggat gcgaccttgc
    23641 gtggctcgat gggcatcggc cttcgggcgg gcctgggcac agtgggagcg gccgctgggg
    23701 tgacgaggag cgcggccccc tcatcgtgtg gaatcagcgg tgggagtatg acaaggcgcc
    23761 gggagacctc ttccgcgccc tgtctgcgct gcgggagcgt ggagtgggct tccggctcgc
    23821 catggcgggg gccaaccacg gtctgccctc ggcccagttc gtcgaggcgc gcgggaggct
    23881 ggattccgag atcgtgcagt ggggtcatgt ggaggatgcc gccgactacg cctcactgct
    23941 gtgggaggcg gatatcgtcg tctccacggc acttcacgag ttcttcggcg tggccgtggt
    24001 ggaggccctt tattgcggct gccggcccgt tcttccccat cgcctaagct acccggagat
    24061 cgttcccaag gaggcgcacg gggaggcgct ctacggaccg ggtgagctgg tggacgcgct
    24121 cgcggaggct ctcgagcgtt ccacggcggc gcgcaagagc gcggggaagt catcccggat
    24181 cgaatggcag aggacctggg tgtcccggta cgattggagc gccatggcat cgcggtatga
    24241 cgcagctgtg caactgtgtt gggagggcgg cctcgcaagg ggcaccaagg agggatgatg
    24301 gaacggatcg cgatcgacta cacgctctgc atggaagaag cactcgggcc tcggaaagga
    24361 ctctcccgct tcctactgga ggctctgcgg ttgccgctcg aggaagcgcg gggtcgtctg
    24421 gccgagaagc ggcacgcggg ggggctcgcc tggatggacc tgcctcgacg cgacgcctcc
    24481 gacgtgctcg acttcgcgga acgggccaca gggcgctacg acgacatcgt ggtgctgggc
    24541 atcggcggct cggccttggg caccaccgcg ctcacctcag ctctgctcca tccgttccac
    24601 aactcgatcc cggcggccgc ccgcggcggg cggccgcgcg tcttcgtcgt ggacaacgtc
    24661 gaccccgacg agataggtag cctgttcacg cacctcgacc tcgagcggac gctcgtgaac
    24721 gtgatctcga agtcgggcac caccgcggag accatggcgg cctacctcgt ggcgcgcggg
    24781 cttctcgtgg agcgcgtggg ggagggtgcg ctgcgggacc atctggcgtt caccaccgac
    24841 ccccacggcg gggtgctcag gaagatcggc agacaggacg gcatacctct gttcgacgtc
    24901 ggtgcgggcg tgggcgggcg cttctcggtg ctcagccctg tgggccttct tcccgcggcg
    24961 ctggcgggga tagacgtggt gggactgctc gccggcgccg ccgagatgga ccagtggatc
    25021 gccgaggccg acgtgatgtc gaacccggcc tatctcttcg ctaccctgca gttcctgcaa
    25081 gacacgcgct tccagcggca cctgagcgtg atgatgccct actcgcgggc gcttcgggac
    25141 gtggcagtgt ggtatcggca gctgtgggcg gagagtctgg gcaaggcggt ggaccggcgg
    25201 ggccgcaccg tgcatgtggg ccccacgccc atcgacgcgc tcggggccac cgaccaacac
    25261 tcgcaggtgc agctctacac cgaaggaccc gacgacaagg tgttcacgtt cctgcgggta
    25321 gacgagttca gctacgacgt ggtcatcccc gacctgcacg ccgcggagga gtcgttggcc
    25381 tacctgggcg gcaggtcgat gtccgacctg ctcaacgcgg agcaggaggc caccgcgtgg
    25441 gcgctcgccc aacgaggacg gccctctctc accatctcgg tgccgcgcgt cgacgccggg
    25501 tcggtgggcc aggtgctcta cctgctcgag gtggccgtct ccgtggcggg ggaactctac
    25561 gacatcaacg ccttcgacca gccgggggtg gagttgggca agcaggcgac gtacgcgctc
    25621 atgggtcggg cgggcttcga ggagctggcc ggacgcattc gcgggacggt gggcggtgac
    25681 ggatcggctg ccggtgtccg ctatctcgtt cactga
//
LOCUS       DASRMJ010000032         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_113344, whole genome shotgun sequence.
ACCESSION   DASRMJ010000032 DASRMJ010000000
VERSION     DASRMJ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113344"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /locus_tag="VFD74_00940"
     CDS             complement(<1..1624)
                     /locus_tag="VFD74_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48151.1"
                     /translation="MKIVEWLRWPFSRQPLPAGTERSEEASPTGLLLYEDAKNDLPGT
                     VFSLLLVAAAGLALWAVLPSFAGGEISSWDGPGHVVRSLIFEKSLTPRSLGIFGLYEG
                     WYLGTPGFLFYPPGFFAVVSLLRGLSAGALSITLATKIVLAFLYVLFPLLIAWLARRL
                     GLSRRAAALAALLSLCFHAHWNQGLTGIYEVGLYTEVLALVGFILVWGLLHGIVWGRP
                     SRGPQGAPAGSAPPSDKGWFSRDVLLGGLALAGVFVTNIVVSSYVPFLLLLYFVCALL
                     WRRSALKGYFALLGIAVLMSFFWIYPFLRSLPYRGPATQWRVRSFTEVMSDLFLKHEL
                     FPWTVTLGAVLGLAALLALLVERRRLSVPLFSPLLLVGLTLVVSSGTFRDAVVPLLDR
                     IPLLAIPSRVVDQAYTVRSQQLLWVALPLFGAAGLSWLLDRLRSARPFLTVLPYVLLL
                     GVLVVVLLFRTGDIRERYVHTFENHPVYAARYDEAQKAAEYLRPLVEPTSVVLVYVGK
                     NGGWLPAIDSVIGLGTGARMVGGHQLEAVHGSF"
     gene            1822..2688
                     /locus_tag="VFD74_00945"
     CDS             1822..2688
                     /locus_tag="VFD74_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48152.1"
                     /translation="MTLLAWPFGRRPAPRREPNPLRSVCAWTPSRAAGVHIAPRRRQA
                     NPQTDFAIETTDLVKVYGGETRTLDGISFTVALGELFGLLGPNGAGKTTAIRILSTLL
                     TPTAGTARVQGFDVVADPREVRRRLGLAMQTPTLDAFSTGRETLKFAGRLHRMPSEET
                     KRRTDELLELMGLASVAKKLTGTYSGGMKRRLDLQAVGVELRLLLPHHHRIDGGLLCL
                     HHRQRFAVVAPQDVVGITNARFVRHARDFVFTILHLIERPTGALQGEVDDEPTGLACS
                     RSGDSLHRRTRP"
BASE COUNT          588 a          893 c          975 g          422 t
ORIGIN      
        1 tgaacgagcc gtgcaccgct tcgagctggt gtccgcccac catgcgtgct ccggtcccca
       61 ggccgatcac ggagtcgatg gcggggagcc agcctccatt ttttcccacg tagaccagca
      121 ccacggaggt cggctcgacc aggggacgga ggtactccgc cgccttctgg gcttcgtcgt
      181 accgggcggc gtacaccggg tggttctcga aggtatgcac gtagcgttcc cggatgtcgc
      241 ccgtgcggaa gagcaggacg acgaccagca cgccgagcag caggacatac gggagaacgg
      301 tgaggaacgg ccgagccgag cggagcctat ccaaaagcca ggagagcccg gcggcgccga
      361 acaaggggag ggcgacccac agcagctgct gggatcgtac ggtgtaggcc tggtcgacca
      421 cccggctcgg gatcgcgagc aacgggatgc ggtccagcag ggggaccacg gcgtcccgaa
      481 aggtgcccga ggacaccacc agggtcagac cgacaaggag gagaggcgag aagaggggaa
      541 cgctcaaccg gcggcgctcg acgagcaggg ccagcaaggc ggcgaggccc aggacggcgc
      601 ccagggtgac cgtccagggg aagagctcgt gcttcaggaa gaggtcggac atgacctccg
      661 tgaagcttcg tactcgccac tgggtggcgg gtccccggta ggggaggctc ctgaggaagg
      721 gatagatcca gaagaacgac atgaggacgg cgatcccgag cagggcgaag tagcctttga
      781 gcgccgaacg ccgccagaga agtgcgcata cgaagtacag gagcaggaga aagggcacgt
      841 agctgctcac cacgatgttc gtgacgaaca ccccggctag ggcaaggcct ccaagaagca
      901 cgtccctgct aaaccagccc ttgtcggagg ggggcgccga gcctgcgggg gccccttgcg
      961 gcccccggct cgggcgcccc catacaatcc cgtgcaggag accccagacc aggataaagc
     1021 ctacgagcgc caggacctcg gtatagaggc ccacttcgta gatgccggtg agcccctggt
     1081 tccagtgggc atgaaagcat agggagagaa gcgcggcgag ggcggcggcg cgtcgggaaa
     1141 ggcctaaccg ccttgccagc caggcaatca gcagagggaa gagcacgtag aggaaggcga
     1201 ggacgatctt cgtggccagc gttatggaga gggctccggc gctcaagccg cgcagcaggc
     1261 tgaccacggc gaagaagccc ggcgggtaga agagaaagcc gggagttccg aggtaccaac
     1321 cttcgtagag gccgaaaatc cccagcgagc gtggagtgag gctcttctcg aatatcagtg
     1381 agcggacgac gtgcccggga ccatcccagg aggagatctc gccccccgcg aaggaaggca
     1441 gcaccgccca gagggccagg ccggcggccg cgacgagcaa aagcgagaag accgtaccag
     1501 ggaggtcgtt cttggcgtcc tcgtagagca ggaggccggt cggcgaagcc tcctcggagc
     1561 gctccgttcc ggccgggaga ggctggcggg agaagggcca ccgcagccac tcgacgatct
     1621 tcatgcgttt cctcctgtgt caagctgaag ggacggaggg cggaacggaa tcgagctcta
     1681 gccgcccgtc tgaccaccag gttccactgc ctcgacgcta ccataccgaa gcccgtacta
     1741 tgccgagctg tctgcgggag ggaagctcga ggctcgaggt taagccctcg agagccgggg
     1801 aaccactacg agaagctgca gatgaccctg ctcgcatggc cgttcggccg gcgcccagct
     1861 ccccgacgcg agcccaatcc acttcggtcc gtttgcgcat ggacgccgtc gagagctgcg
     1921 ggcgtccaca tagctcctag gagacgacag gcgaatcctc aaaccgattt cgccatcgag
     1981 accaccgatc tcgtcaaggt ctacgggggt gagacccgga ccctggacgg aatcagcttc
     2041 acggtcgcgc tcggcgagct cttcggcctg ttggggccaa acggtgcggg caagaccacg
     2101 gccatccgca ttctctccac cctgctgaca cctaccgccg ggaccgctcg ggttcagggc
     2161 ttcgacgtcg tcgcggaccc gcgagaggta agacggcggt tgggcttggc catgcagact
     2221 cccacgctcg acgccttctc gaccggtcgt gagacgctca agttcgcggg ccggctccac
     2281 cgcatgccct ccgaagagac aaagcgacgg acggacgagt tgctcgagct catggggctg
     2341 gccagcgtgg ccaagaagct caccggcacc tactcgggag gcatgaagcg tcggctcgac
     2401 ctccaggcag tcggcgtaga gcttcgcctg ctgctgcccc accaccaccg gatcgacggt
     2461 ggtctgcttt gcctccacca ccgccagcgg tttgccgtcg tcgccccaca ggacgtagtc
     2521 ggcataacca acgcccgatt cgttcggcat gcccgtgact tcgtattcac gatcctgcac
     2581 ctgatcgagc ggccgaccgg cgcgcttcag ggcgaggttg atgatgagcc tacgggtctt
     2641 gcctgttctc ggtcaggtga ttcgctacat cgcagaactc gaccgtgacc gcgacaagat
     2701 cctcagcaag gaccggtgcg acacactgaa gatacgcgct cgagtgattg tcgggcgtga
     2761 cggtgacttg gggcatcaag aaggcctgcg gaatctgaat gcgcacctac atcggattga
     2821 ggttgtcacg ttcgatcagc ttgttcgtat cgcagccagg gtgcttgatg tgttcgag
//
LOCUS       DASRMJ010000033        11236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_402271, whole genome shotgun sequence.
ACCESSION   DASRMJ010000033 DASRMJ010000000
VERSION     DASRMJ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11236
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402271"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFD74_00950"
     CDS             complement(<1..538)
                     /locus_tag="VFD74_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZK48153.1"
                     /translation="MDIGIIGLGKMGANMARRLVAGGHRVVVANRSPEPVAQLATEGA
                     VPARDLAELVGLLSPPRAAWIMVPAGDPTEKMVLDLADLLTEGDVIVDGGNSNYKDSM
                     RRAATMAERGLGFVDAGTSGGIWGRTEGYSLMVGGTEESVALVRPALESLAPAADLGW
                     GHVGPAGAGHFVKMVHNGI"
     gene            complement(674..1906)
                     /locus_tag="VFD74_00955"
     CDS             complement(674..1906)
                     /locus_tag="VFD74_00955"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HZK48154.1"
                     /translation="MSATGERQPMKRLEATRSLRSLIKGYYDDLFRTASEGRPSAWLN
                     VGVPCEFFYAMDIFPFYPENYGAACGALKITPQLSAVSEAHGFSADLCSYVRATLGTV
                     FSGEGPYGELPKPTLQVSAMNSCIMIMVWWRAVQRHWEVPTFVVDTPLVRDQSEGINL
                     DYVESELRRMVAWVEGQTGRVLDLERLREVVRLSNEGKEIWSEVLDLRRARPCPITAA
                     DIFTHMFPMVALRGTPQYVAHLRALRDEIKQRVDEGFAAVEGERYRLLWDNLPPWFDL
                     KLFDDLAARGVNFVIDTYTQAWGPRYMGPIDEVDPLRSFAAYMGAGFLNVQIGRRYDL
                     LAELVDLYGVDGVVFHSDRSCKPFSLVQVELRRKLQENLGIPGLLLEGDHNDQTLLDR
                     GRAWSQLESFLEVIDGRR"
     gene            complement(1903..3135)
                     /locus_tag="VFD74_00960"
     CDS             complement(1903..3135)
                     /locus_tag="VFD74_00960"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HZK48155.1"
                     /translation="MSGRAHTGEPPAGPGAMGSAHGASGAGAAALARLRSVGTDPLSE
                     LAHIKERTGLMAMGYFDSYLPEELIMAHGIVPHRVTVSGRDDPRSHEFIQGYACPAAR
                     DLLEQALRGDLSLLDGVLFTRYCDSLRGVFAVWDSQKLSPFVDFVRYPTVVDTEGAVT
                     YLAQELQEVSARIARALDAPLDERRLLDSIAACDRKRALVAELAARRATRDLPLAGAD
                     FLAVLVAATTMLPDEFTAALSTLLEHPPAGEPGDAVPVVLSGTTFDNIALAAAIESAG
                     LYLAADDLSTGSRWYGIRVGSQPAAAGETPTDPWEALARAYLTKPPCSVKEPSSPRAD
                     HLVGLVRASAAEGVLFYLTKFCDSEQVEWPFLRDRLAREGIPVMMVEGDQKGEGSGAL
                     STRLEAFRERFELGGEDS"
     gene            complement(3132..4688)
                     /locus_tag="VFD74_00965"
     CDS             complement(3132..4688)
                     /locus_tag="VFD74_00965"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HZK48156.1"
                     /translation="MNIADALDRNAIFFPDHEALVDGARRWTYGAFRRDADRVAHALT
                     ALGVVKGDRVCLFMGNRAEFAVAFYGILKVGAIAVSISSMCKTGEVEYMVNNCEAEVV
                     IAGSDYTGEMPERGDIPRVRHVIVVSPVGSADGDSGAAGAPPSAYGADHDFWSLLEEH
                     DEVFPTVYMDREDGAEIIYTSGTTGKPKGVVLTHGNVVSNTYSTVYEGGISRNDRTIC
                     FLPMYHSFAQNFIFNGTVRAAATLVILPKFEIETVLRTMAEEKITRWHAVPTIYIIVL
                     GTPGAEEAFKHVRFCFSAAATMPEEIARQWYDRFGLKINEGYGLTESTPSAVYNHTVR
                     HKAGTVGTPIMNVEVQIWDADGKVLPAGGVGEIVLKGPNIMKGYYNDPVATAETLVDG
                     WLKTGDVGMLDEEGYLSIVDRIKDMVNSAGLKIWPREVEEVLYTHEAIEECAVIGVSD
                     PVYGENVKACIVLRSGATLCAEEVVVFCKQRLSAYKAPKIVEFMETLPKSATGKILKI
                     ELRKMSAGSE"
     gene            complement(4685..5680)
                     /locus_tag="VFD74_00970"
     CDS             complement(4685..5680)
                     /locus_tag="VFD74_00970"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZK48157.1"
                     /translation="MGLRIAIVGAGAIGGTLGFLLAEAGYDVTLVDIDAAKVALLQEE
                     GLTVIMPDGRERWRLMKATTDPGTVGVVDLVQISVKGYQTTAAAETARPLVGPGTYVL
                     SLQNGLKNLQRIAAVLEDANVVGGVTAHSAMPLGPNVIKYNGGLGGISIGRFDGVDDP
                     GLQRLIDAFSAAGIPTTRIAGDVRIPIWRKLLANVSCNAVAALTGFTGRQLVEFQPTN
                     ELIRALAEETAEVARAQGFDFAELEFAGDFAIKALSGVGDNKISMLQDVEAGRRTEID
                     TLNLAVVERGEMHGVDAPLNWMIGILIQALEQRMLFEREARAAAPAGDETRSTPE"
     gene            complement(5798..6565)
                     /locus_tag="VFD74_00975"
     CDS             complement(5798..6565)
                     /locus_tag="VFD74_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZK48158.1"
                     /translation="MGNGVDSSVSGGIGRLILDNPPVNVFTIAVMEAAVEALRGLEAD
                     PSVKVIAVQATGERAFSAGVDVADHTPDKMRHMLDIFDELCMGLHRSAKPTVALVRRM
                     ALGGGAELVACCDMAIASDEATFGQPEIKVGVFPILGVALFPDMLGPKAAAELLLSGD
                     TYSAADALRMGLVSRVVPEAEFEEKSERFLSKLARNSGLVMSLTKKAMLAGLGKEASE
                     SLRLADAIYVDELMLTEDANEGLKAFIERRAAEWKER"
     gene            complement(6633..7430)
                     /locus_tag="VFD74_00980"
     CDS             complement(6633..7430)
                     /locus_tag="VFD74_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZK48159.1"
                     /translation="MKLRALIVDDEPRARSHLRDLLGDIENVELVGEASTAAEAEKLL
                     LAVEHDLVFLDIRMPGMDGMSAAEIFLALPKKPFVVFTTAYDEYAARAFELGAADYLL
                     KPISRARLLKAVNRARELKAGGEAAVIISEPVADAASDGGRSGTTPSGVGKRLEYVTA
                     RDGARLILVRVEDIVFLSVADETVHVTTGADTMVALAPSMDELEQSLAGLDFFRVHRG
                     YLVNLRKVSEVVPLNNRTYELVMKDRKASRVPVSRRKAVELRAILGF"
     gene            complement(7539..8699)
                     /locus_tag="VFD74_00985"
     CDS             complement(7539..8699)
                     /locus_tag="VFD74_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZK48160.1"
                     /translation="MNIGSDAVFEIVNLTLPHLREGLSFRSAAAAASAIHEVVGASAT
                     SISDSQKILAFVGKGDDHHKVGLPILTHLTRETLSSGKVTAVRGRMSIGCPVETCALR
                     AAIVVPLKARLQIVGCLKFYNTVDLPFSQKEIQFADGLANMLSIELELAEIDIQKKLA
                     TQAELQALQAQISPHFLFNTLNTIASYCRHDGLLAEELLVEFADLFRRNLKQHRGFVS
                     LREELDFVDRYLCFEQYRFGSRLHIERLHQPAALETLVPPLILQPLVENAVTHGVTRK
                     VGSSRVVIRSWVTDDEAVIAVEDDGVGMDSASFRGGGGGIGMRNVQRRLVGLFGPQYG
                     LVVRSPDTGGTVVEVHLPRRISPAVYSLIQEERRGDPAGVSNQPDFAEVDFR"
     gene            complement(8750..9025)
                     /locus_tag="VFD74_00990"
     CDS             complement(8750..9025)
                     /locus_tag="VFD74_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48161.1"
                     /translation="MTTTRTASELRNRLRAEGWEERFSAAGTRLEEAAEHYRLLGYEV
                     RIEGIVESAEDGACTSCFGVVGADGPVGVIFTRGGIAAAQPEEDLFD"
     gene            complement(8976..10178)
                     /locus_tag="VFD74_00995"
     CDS             complement(8976..10178)
                     /locus_tag="VFD74_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZK48162.1"
                     /translation="MDIAMVSVFVDGSAVEVPVGSTILDACDRAGVYVPRLCAYPGLE
                     PLGDCGLCLVRVDGGDARRACSVTVAQGMSVDTRDEEALRFRAGSMRAILGDHPHVCL
                     TCPERDGCSRDQCTYGEPPEARCCREFGHCEIAKVAFFVGALEAAPAYVHRRLGGPVV
                     DHRIHRDLYLCVGCGRCVAACDSLEEAGSALELVETRGLVRVAAVAEGVSPASAAADA
                     TATAGWQPHSYLGRHLAVPKEADLRASGCTFCVACVMACPSGALTAEGSGGAAWLAKR
                     RERSTLRPAALPPEKRLPYDEEALAGVPAAEGVFRLFSATGELLQVSGVRDLARGLRE
                     AAGGSVGEEVRFFDFEVEPLYTQRESEFLARHLQEHGSMPRGNDILGELFDDDDENSL
                     GASQPTPS"
     gene            complement(10197..10478)
                     /locus_tag="VFD74_01000"
     CDS             complement(10197..10478)
                     /locus_tag="VFD74_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZK48163.1"
                     /translation="MIANYGYRDGSGDYYITIDTDRCVECEGRWCVAACPESLFQIVT
                     DDYDDEVAIIVAERRKMLKERCSGCKSAGGYTDPPCTTACRPGAVEHSW"
     gene            complement(10578..>11236)
                     /locus_tag="VFD74_01005"
     CDS             complement(10578..>11236)
                     /locus_tag="VFD74_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HZK48164.1"
                     /translation="VSYVVGTGYGRVNVPFADRAITEIACHARGANAMVGPSVRTILD
                     MGGQDCKVIRCDEKGKVQNFIMNDKCAAGTGRGMEVIADTLGVPIGDIGRRSFEIDEE
                     PPPVSNVCTVFAKSEATALLKAGWSLNRVLASYCSAMAERVVGLIDRMGMEPEFFITG
                     GIAKNEGVVRRIERKLGIAAIRLPPDTDFDPQIAGALGAALFAAALYRKQQKAAAGTV
                     "
BASE COUNT         1886 a         3887 c         3599 g         1864 t
ORIGIN      
        1 cgatgccgtt gtgcaccatc ttcacgaagt gaccggctcc cgcgggccct acgtgccccc
       61 aaccgaggtc ggccgccggc gcgagagact cgagtgcggg gcgcacgaga gccaccgact
      121 cctccgtccc accgaccatg agactgtagc cttccgtccg cccccagatc ccgccgctgg
      181 tccccgcgtc gacgaagccg aggccccgct cggccatggt cgcggcacgg cgcatcgagt
      241 ccttgtagtt ggagttgccc ccgtcgacga tcacatcgcc ctcggtgagc agatcggcga
      301 gatcgagcac catcttctcc gtggggtctc cggcgggcac catgatccac gccgcgcggg
      361 gcggggagag caacccgacc aactccgcca ggtcgcgggc gggaacggcg ccctcggtcg
      421 ccagctgagc cacgggttcc ggagagcggt tggcgaccac cacgcgatgg ccacccgcca
      481 ccaggcgtcg cgccatgttg gctcccatct tgccaagacc gatgatgccg atgtccatgg
      541 tgaagctctc cttggatcga gagccgtcga ctcaggtcgt cgcggctcgg ttcgagacta
      601 gataggtggt cttcgcggcc ggtcggtact cgtggacgtc cgtgggccgg cgccgcgcac
      661 ccgcgtcacg cgcctatcta cgcccgtcga tcacttcgag gaagctctcg agctgactcc
      721 acgccctgcc tcggtcgagc aaggtctggt cgttgtggtc gccttccagc agcagccccg
      781 ggatgcccag gttctcctgc agcttcctgc gcaactccac ctgcaccagg ctgaacggct
      841 tgcagctgcg gtcggaatgg aacacgacgc cgtcgacgcc gtagaggtcg accaactcgg
      901 ccagcaagtc gtagcgccgc ccgatctgga cgttcaggaa gccggcgccc atataggcgg
      961 caaaggagcg caggggatcg acctcgtcga tcggccccat gtaacgagga ccccaggcct
     1021 gggtgtaggt gtcgatcacg aagttcacgc cgcgggcggc aagatcgtcg aagagcttca
     1081 ggtcgaacca gggaggaagg ttgtcccaga gcaggcgata gcgctcgccc tccacggcgg
     1141 cgaacccttc gtctacccgc tgctttatct cgtcgcgaag agcgcgcagg tgcgccacat
     1201 actgaggcgt gcctcgcagc gcgaccatgg ggaacatgtg ggtgaagatg tccgcggcgg
     1261 tgatggggca tggtctggcc cgcctgagat cgaggacctc cgaccagatc tccttgccct
     1321 cgttcgagag ccgaacgacc tcgcgcagcc gctccaggtc gagcacgcga ccggtctgcc
     1381 cttccaccca cgccaccatg cgccgcaact ccgactccac gtagtcgaga ttgatgccct
     1441 cggactggtc gcgcaccagg ggcgtgtcga ccacgaaggt gggcacctcc cagtgacgtt
     1501 gcaccgcccg ccaccagacc atgatcatga tgcatgagtt catggcgctc acctggagcg
     1561 tgggcttggg aagctccccg tagggcccct cgcccgagaa cacggtgccc aaggtggccc
     1621 gcacgtagga gcagaggtcg gcgctgaagc cgtgcgcctc cgacaccgcg gagagctgcg
     1681 gagtgatctt gagagcgccg cacgcggctc cgtagttctc cggatagaac gggaagatgt
     1741 ccatcgcata gaagaactcg caggggaccc ccacgttcag ccaggcgctc gggcgccctt
     1801 cggaggccgt gcggaagagg tcgtcgtagt agcccttgat cagcgatctc aggctccgcg
     1861 tagcctccaa ccgcttcatg ggctgccgtt cgccggtggc gctcacgagt cctcccctcc
     1921 caactcgaat cgctcccgga aggcttcgag ccgcgtgctc agggcgccgg acccctctcc
     1981 cttctggtcg ccctcgacca tcatcaccgg gatgccctcg cgggccaggc gatccctcaa
     2041 gaagggccac tccacctgct cggagtcgca gaacttggtg aggtagaaaa gcacgccttc
     2101 ggccgcgctc gcgcgcacca accccacgag gtggtcggcc cgaggtgacg agggctcctt
     2161 gacggagcag ggaggcttag tcaggtaggc gcgggccaag gcttcccaag ggtcggtcgg
     2221 ggtctcgccg gccgccgccg gctgcgagcc caccctgatg ccgtaccagc gcgaacccgt
     2281 ggagagatcg tcggccgcca ggtacaaccc ggccgactcg atagcggcgg cgagggcgat
     2341 gttgtcgaag gtggtgcctg agaggaccac cggcaccgca tcgccgggct cgccggccgg
     2401 cggatgctcg aggagcgtgg agagcgccgc cgtgaactcg tcgggcagca tcgtggtggc
     2461 ggcgacgagc accgccaaga agtcggcgcc ggccagcggc aggtctcgcg tggcccggcg
     2521 ggccgcgagt tccgccacca gtgcacgttt tcggtcgcac gcggcgatgg agtcgaggag
     2581 cctgcgctcg tcgagcggag cgtccagcgc ccgggcgatc cgcgctgaga cttcctggag
     2641 ttcctgcgcg aggtaggtga ccgccccctc ggtgtccacc accgtcgggt accggacgaa
     2701 gtccacgaac ggggagagct tctgcgaatc ccacacggcg aagaccccgc ggagggagtc
     2761 gcagtagcgc gtgaagagca ctccgtcgag cagagagaga tcaccgcgga gggcctgctc
     2821 cagcaggtcg cgcgcagcag ggcacgcgta gccttggatg aactcatgag accggggatc
     2881 gtcccggcca ctcacggtga cccggtgggg gacgatcccg tgggccatga tcagctcttc
     2941 gggcaggtag ctgtcgaagt agcccatggc catgagaccg gtgcgctcct tgatgtgggc
     3001 caactcggag agggggtcgg tgcccaccga ccgcaggcgc gccagcgcgg ccgcgccggc
     3061 gccggaggcg ccgtgggcgg agcccatggc gcccgggccg gcgggcggct cccctgtgtg
     3121 ggcgcggccg ctcactccga gcccgcggac atcttccgca gttctatctt cagtatcttg
     3181 ccggtcgcgc tcttgggcag ggtctccatg aactccacta tcttgggagc cttgtacgcg
     3241 gagaggcgct gcttgcagaa gaccacgacc tcctcggcac agagcgtcgc gcccgagcgc
     3301 agcacgatgc aggccttgac gttctcacca tagaccgggt cggaaacacc gataacggcg
     3361 cactcctcga tggcctcgtg ggtgtagagc acctcctcca cctcgcgcgg ccagatctta
     3421 agaccggcgg agttcaccat gtccttgatg cggtccacga tggagaggta accctcctcg
     3481 tcgagcatac ccacgtcacc cgtcttgagc cagccgtcca ccagggtctc cgcagtcgcc
     3541 acgggatcgt tgtagtagcc cttcattatg ttcgggccct tgaggacgat ctcgcccacg
     3601 ccgcccgccg gaagcacctt cccgtcggcg tcccagatct gcacctcgac gttcatgatg
     3661 ggggtgccca ccgtccccgc cttgtgacgc accgtgtggt tgtacacggc cgacggagtg
     3721 gactcggtga gcccgtatcc ctcgttgatc ttaagaccga aacggtcata ccattggcgc
     3781 gcgatctctt cgggcatggt cgcggcggcc gagaagcaga acctcacgtg cttgaacgcc
     3841 tcctccgctc cgggagtgcc gagcacgatt atgtagatgg tgggcaccgc atgccagcgt
     3901 gtgatcttct cctcggccat cgtgcggagg acggtctcta tctcgaactt gggcaggatc
     3961 accagcgtgg cggcggcgcg cacggtgccg ttgaagatga agttctgcgc gaacgagtgg
     4021 tacatgggga ggaagcagat ggtccgatcg tttcgactga tgccgccctc gtagacggtc
     4081 gagtaggtgt tggagaccac gttgccgtga gtgagcacaa cgcccttggg cttgccggtg
     4141 gtgcccgagg tgtagatgat ctccgcgccg tcctcgcggt ccatatagac cgtcgggaag
     4201 acttcgtcgt gctcctcgag cagcgaccag aagtcgtggt ccgctccgta ggccgatggg
     4261 ggggcgccgg cggcgcccga gtcgccgtcg gcggagccga ccggcgagac cacgatcacg
     4321 tgccgcacac gcgggatgtc gcctcgctcc ggcatctccc cggtgtagtc actcccggcg
     4381 atcacgacct cggcctcgca gttgttgacc atgtactcca cctcgcccgt cttgcacatg
     4441 gaggagatgg agacggcgat cgctcccacc ttcaggatgc cgtagaaggc caccgcgaac
     4501 tcggcccggt tgcccatgaa gagacacacc cggtcacctt tgaccacgcc cagagccgtg
     4561 agagcgtggg ccacgcggtc cgcgtcgcga cggaaggccc cgtaggtcca gcgacgggcg
     4621 ccatcgacca gggcctcgtg gtcggggaag aagatggcgt tgcggtcgag tgcgtcggcg
     4681 atgttcactc cggtgtgctc cttgtctcgt cgcccgccgg cgccgccgcg cgggcctcgc
     4741 gctcgaacag catccgctgt tcgagcgcct gtatcaggat gcctatcatc cagttgagcg
     4801 gggcgtccac cccgtgcatc tctccccgct ccacgacggc gaggttcaac gtatcgatct
     4861 cggtgcgcct tccggcttcg acgtcttgga gcatggagat cttgttgtcg cccaccccac
     4921 tcagcgcctt gatggcgaag tctcccgcga actccagttc ggcgaagtcg aacccctgcg
     4981 cacgggccac ctcggcggtc tcttcggcca gcgcccggat gagctcgttg gtgggctgga
     5041 actccaccag ctggcgcccg gtgaatcccg tgagagcggc taccgcgttg caggacacgt
     5101 tggcgagcag cttgcgccag atggggatgc gcacgtctcc ggctatgcgg gtggtcggga
     5161 tgcctgccgc cgagaacgcg tcgatcagac gctgcagacc cggatcgtcc accccgtcga
     5221 agcggccgat gctgatgccg ccgagtcctc cgttgtactt gatgacgttg gggccgagag
     5281 gcatcgcgct gtgcgccgtg acgccgccca cgacgttggc atcttcaagc accgccgcta
     5341 tccgctgaag gttcttcaga ccgttctgca gcgagaggac gtaggtcccc gggccgacga
     5401 gcggtcgagc ggtctcggcc gcggcggtcg tctggtagcc cttcaccgaa atctgcacca
     5461 gatcgaccac tccgaccgtg cccgggtcag tggtggcctt catgagccgc caccgttccc
     5521 ggccgtcggg catgatcacc gtgagcccct cttcttgcag aagggcgacc ttggcggcgt
     5581 cgatgtcaac cagtgtcacg tcgtatccgg cttcggccag gaggaatcca agcgttcctc
     5641 cgatggctcc cgcacccacg attgcgatcc tcaatcccat cgcggacccc cttgacgcct
     5701 cccagtcgtc tggtcggctg tcgtgcgcaa tgatcctacg gtcccgattg tagcgccggc
     5761 ccgccggcgg cgggctcggg ctatcgccgt cggcgctcta tcgttccttc cactccgcag
     5821 cgcggcgctc gatgaaggcc ttcagtccct cgttggcgtc ctcggtcaac atgagttcgt
     5881 cgacgtatat cgcgtcggcc aagcgaagcg actccgaggc ctccttgccc agcccggcca
     5941 acatggcctt cttggtgagg ctcatgacca acccgctgtt cctggccagc ttagacagga
     6001 agcgttccga cttctcctcg aactcggctt cggggaccac gcgactgacc aggcccatgc
     6061 ggagcgcgtc ggccgccgag taagtgtccc cggacagaag cagctcggcc gccgctttcg
     6121 ggccgagcat gtcggggaag agcgcgacac ccaggatcgg gaacacgccc accttgatct
     6181 ccggctgacc gaaggtggcc tcatccgacg ctatcgccat gtcgcagcag gcgaccagtt
     6241 ccgcgccgcc cccgagagcc atgcgccgca ccagcgccac cgtgggcttg gcagagcggt
     6301 gaagtcccat gcagagctcg tcgaagatgt cgagcatgtg gcgcatcttg tctggcgtgt
     6361 ggtcggccac atcgacgccg gccgagaagg cgcgttcgcc tgtcgcctga acggcgatca
     6421 ccttgacgct cggatctgct tcgagtccgc gcagagcctc caccgccgcc tccatcacag
     6481 cgatggtgaa cacgttgacc ggcgggttgt cgagtatcag acgaccgata cccccggaga
     6541 cactggaatc cacaccgttg cccactgacg atcacccctc ttgtctgact ttacgccttc
     6601 tcaactaccc acggccgcgg tcgcgaccgc cctcagaacc ccaagatcgc ccgcaactcg
     6661 acggccttgc gccgactcac cgggacgcgg ctggccttgc ggtccttcat caccagctcg
     6721 taggtgcggt tgttgagcgg aacgacctcc gacaccttcc tcagattcac aaggtatccg
     6781 cggtgaacac ggaaaaagtc gagaccggcc agactctgtt ccagctcatc catggagggc
     6841 gctagagcca ccatggtatc tgctcccgtg gtcacgtgca cggtctcatc ggccacgctc
     6901 aggaagacga tgtcctccac ccgcacaagg atcaaccgag cgccgtctcg cgcggtcaca
     6961 tactcaagcc gcttcccgac ccccgaagga gtggtgccag agcgcccccc gtcggaggcc
     7021 gcgtccgcga cgggctccga gatgatcacc gccgcctctc cgccggcctt gagttcgcgg
     7081 gctcgattca ccgccttcaa cagacgcgcc ctgctgatcg gcttcagcag gtagtcggcc
     7141 gcgccgagct cgaacgcccg ggccgcgtac tcatcgtagg cggtggtgaa caccacgaaa
     7201 ggcttcttgg ggagggcgag gaagatctcg gccgcgctca tcccgtccat gcccggcatc
     7261 cggatgtcga ggaacacgag gtcgtgctcc accgcgagga gcagcttctc ggcctcggcc
     7321 gcggtggaag cctcccccac gagctcgacg ttctcgatgt ctccgagaag gtcgcgcagg
     7381 tgcgaccgcg cccgcggttc gtcgtccact atgagcgcgc gcaacttcac tgcaccggcc
     7441 tcccgcggtc catggacgca ctatgcctca tcgaacgggc cgcccatgac gtccgctgtc
     7501 atgctgctca cggctgcggg cggcttcgca agttggattc accgaaagtc cacctcggcg
     7561 aaatcaggct gattcgagac gcccgccggg tcccctctcc gctcctcttg tatcagcgag
     7621 tataccgccg gagaaatgcg ccgcgggagg tgtacctcga ccaccgtgcc gccggtgtcc
     7681 gggctgcgca ccacgagtcc atactgcggt ccgaagagcc cgacgagacg gcgctggacg
     7741 ttgcgcatac cgatcccccc gccgcctcca cggaacgatg ccgagtccat acccacgccg
     7801 tcgtcctcga ccgcgatcac cgcctcgtcg tcggtgaccc agctgcggat cacgacccgc
     7861 gaggaaccca ccttcctggt gaccccatgg gtgacggcgt tctccaccaa cggctggagg
     7921 atcagcggag gcaccagcgt ctccagcgcg gccggctggt gcaaccgctc gatatggagt
     7981 cgcgagccga aacgatactg ctcgaagcac aggtagcgat cgacgaaatc aagttcttcc
     8041 cggaggctca caaaacctcg gtgctgcttg agattgcgtc tgaacagatc tgcgaactcg
     8101 accaggagct cctcggctag gagaccgtcg tgccgacagt acgacgcgat ggtgttgagg
     8161 gtgttgaaga gaaaatgggg actgatctgg gcctgcaagg cctggagttc cgcctgcgtc
     8221 gcgagcttct tctggatgtc gatctccgcc agctccagct cgatggagag catgttcgcg
     8281 agaccgtcgg cgaactggat ctccttctgt gagaacggca ggtcgacggt gttgtagaac
     8341 ttgaggcagc ccacgatctg caggcgggcc ttcaagggca cgacgatagc cgccctgagc
     8401 gcacaggtct cgacggggca gccgatggac atgcgaccgc ggaccgcggt gaccttcccg
     8461 ctactgaggg tctcccgcgt gaggtgggtg aggatgggga ggccgacttt gtggtgatcg
     8521 tcccccttgc ccacgaaggc caggatcttc tgcgagtcgg agatcgacgt agccgaagcc
     8581 cccacaacct catggatggc gctggcggcc gccgcagcgc tgcggaaact gagcccttcg
     8641 cgaaggtgcg gaagggtgag gttcacgatc tcgaagacgg catcagagcc gatgttcacg
     8701 tgcgatccac cgctgctgtc ggcccgaagg ctcctgtcac gatgcgaatc tagtcaaaga
     8761 ggtcctcttc cggctgcgcc gctgcgatcc cgccccgggt gaagatcacg cccaccggac
     8821 cgtcggcccc cacgacgccg aagcaactcg tgcaggcgcc gtcctccgca gactccacga
     8881 tcccctcgat gcggacctca tagcccagca agcggtagtg ttccgcggcc tcttcgagcc
     8941 gcgtcccggc cgcgctgaaa cgctcttccc agccctcagc tcggagtcgg ttgcgaagct
     9001 ccgaggctgt tctcgtcgtc gtcatcgaac agctccccga gtatatcgtt cccgcggggc
     9061 atgctgccgt gttcctggag atgccgcgca aggaattcgc tctcccgctg agtgtacagg
     9121 ggttccacct cgaagtcgaa gaagcggact tcttcgccga ccgaccctcc cgcggcctct
     9181 ctgagcccgc gggccagatc tctgacaccc gagacctgaa gcagctcgcc ggtggccgag
     9241 aagagccgaa agaccccttc cgccgcgggg acgcccgcca gagcctcctc gtcgtagggc
     9301 agtcgcttct ccgggggcag cgcggcgggc cgaagcgtgc tccgttccct gcgcttcgcc
     9361 aaccaggcgg ccccgccgct cccctcggcc gtcagagccc cggaggggca cgccatgacg
     9421 caggcgacac agaacgtgca gcccgaggcg cgaaggtccg cctccttggg caccgccagg
     9481 tggcggccga ggtacgagtg gggctgccag ccggcggtcg cggtggcgtc ggcggcggcg
     9541 gaagcggggg agacgccctc ggcgaccgcc gcgacacgga ccagaccgcg ggtctccacg
     9601 agttcgaggg ccgatccggc ctcttcgagc gagtcacagg ccgcgacgca ccgtccacac
     9661 cccacacaga ggtagaggtc gcggtggatg cgatggtcga ccacggggcc gcccaggcgc
     9721 cgatgaacgt acgcgggcgc cgcctccagg gcgccgacga agaacgccac tttggcgatc
     9781 tcacagtgcc cgaactcacg acagcatcga gcctcggggg gctcgccgta ggtgcactgg
     9841 tcgcgactgc aaccgtcgcg ctccggacag gtgaggcaca cgtgcgggtg atctcccaag
     9901 atggccctca tcgaccccgc gcggaaccgc agtgcctcct cgtcgcgggt gtcgacactc
     9961 atgccctggg ccacggtgac ggaacaggcc cggcgcgcgt cgccgccgtc gaccctgacg
    10021 agacagagtc cgcaatcccc gagaggctca agaccgggat acgcgcagag ccgcggaacg
    10081 tagactccgg cacggtcgca ggcatccagg atggtagaac ccaccggcac ctcgaccgcc
    10141 gacccatcga cgaacacgct gaccatggcg atatccacca gcgaccctcc tcccgcctac
    10201 cagctatgtt caacggcgcc ggggcggcag gccgtcgtgc agggaggatc ggtgtacccg
    10261 ccggccgact tgcagccgga acaccgctcc ttcagcatct tccgcctttc cgcgacgatg
    10321 atagccacct cgtcgtcgta gtcatcggtg acgatctgga agagcgattc gggacaagcc
    10381 gccacgcacc accgaccctc gcactcgacg cagcgatccg tgtcgatggt gatgtagtag
    10441 tcgcccgacc cgtcccggta tccgtagttg gcgatcacgg ctcccccctc cgtccctcgg
    10501 tgcatccgac agacacatcg ctgcgtcgcc cgttcgcggc gggcgtggcg ggcgcgtcga
    10561 gcccccgccg gtcgacgtca gaccgttccc gcggcggcct tctgttgctt ccggtagagc
    10621 gctgccgcga acaacgcggc cccgagggcg cccgcgatct gcggatcgaa gtcggtgtcc
    10681 ggagggaggc ggatagccgc gatccccagc ttccgctcga tgcgcctcac cacaccttca
    10741 ttcttagcga tacctccggt gatgaagaac tcgggctcca tccccatgcg gtcgatgaga
    10801 ccgaccacgc gctcggccat cgccgagcag tacgaagcaa ggacgcggtt gaggctccaa
    10861 cccgccttga ggagggccgt ggcttccgac ttcgcgaaca ccgtgcatac gttggagacg
    10921 ggcgggggtt cctcgtcgat ctcgaaggaa cgtcggccga tgtccccgat gggcacaccc
    10981 agcgtgtcgg cgatcacttc catgccccgt cccgtgcccg ccgcgcactt gtcgttcatg
    11041 atgaagttct gcactttgcc cttctcgtcg cagcggatca ccttgcagtc ctggccgccc
    11101 atgtcgagga tggtgcgcac cgacgggccc accatggcgt tggcgccgcg ggcgtgacag
    11161 gcgatctcgg tgatggcgcg atcggcgaag ggcacgttca cgcgcccgta ccccgtgccc
    11221 acgacgtagc tgacgt
//
LOCUS       DASRMJ010000034         4175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_364134, whole genome shotgun sequence.
ACCESSION   DASRMJ010000034 DASRMJ010000000
VERSION     DASRMJ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364134"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VFD74_01010"
     CDS             <1..1084
                     /locus_tag="VFD74_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48165.1"
                     /translation="PSSFLVVERERETLAAIDRAMGGVGRVGCADGVDGRAIGRADVG
                     LRSAGEVADTLARLERSDRSGARVPLTFPSEPWSARTFFNLAPILADADSGANGFVIA
                     VHPDYEELDLGCLLRPEPRTGAAVPSGASPLGASALPLGSGASPCPPSRILPSAALEA
                     VVWSWALLQRWADHRALLSIPFINGGKDSASGQSLACFHAQFYALAADPPPPSYGCSR
                     GWAAAEECPLCALRDVGGFEVTRVGSVGVFAHPAPEIELTLLVASLGHAGGITDLPDP
                     ADLAKALALAVRSYEHLLGGVPPYNIVVRTGAGAGHLHAEVMPRAGVPVAAGFEKATG
                     LFVSTRDPRVLAEGLRAWVEGRAPAP"
     gene            1081..1947
                     /gene="otsB"
                     /locus_tag="VFD74_01015"
     CDS             1081..1947
                     /gene="otsB"
                     /locus_tag="VFD74_01015"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HZK48166.1"
                     /translation="MSPPESRGRGPAPGSPAAGSPAAGRLPAELHAALQRLAGLPGRS
                     ALFFDVDGVLASIRPRPEEAAVPRTVRRLLVDLVPAYRLVAAVSGRSLEEAVAMVDTP
                     GVVVVGDHGRETRDEHGRVTRSRAAERGLIREAAAVLGGDAGLRRAGVRIEEKPASIA
                     LHVRGAADIARAETAALEAAAAVGRRFGLTVRQGRSVIDLKIPGVDKGTAVERLVARY
                     GVESVLYVGDDTTDVDAMRALARMRSDVFFAVLLGVSSNEMPAELAAASDHLVRQREV
                     ASVLRALAPPRV"
     gene            complement(2068..2493)
                     /locus_tag="VFD74_01020"
     CDS             complement(2068..2493)
                     /locus_tag="VFD74_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZK48167.1"
                     /translation="MSGKSDFFSHQRYALVGDSRDKKFPHFTHKYLMEQGKTVYPVDL
                     SGGQAGFLGSLAEVPEDTEAVIIEVAKEKTAGTVSEALDRGFKRIWLHQMTDTPEALQ
                     LAAAAGAAVESGGCAVMYLAPTLSPHALHRGIWKLVGRY"
     gene            2694..3887
                     /locus_tag="VFD74_01025"
     CDS             2694..3887
                     /locus_tag="VFD74_01025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZK48168.1"
                     /translation="MIGTADGIFQNLAAGVRDSAFRVRFWDGTERTYGDGDPRFTVAF
                     DSREALDAVLTLGSLGTGEAYMEGALDVEGDLHEFFRVGSHPSVRSPRLGTRDKAMIV
                     AAYVRNRNTLRKARENIEHHYDLGNDFYRLWLDEGMTYSCAYWDETCDGDLGAAQRAK
                     YEHICRKLHLRAGDRLVEFGSGWGGMLAHAGREYGVEGVGCTLSTQQHEYASERFVAE
                     GLHPRVRSELIDYRKMEGTFDKFVSIGMFEHVGREYYSTFFEKVRALLVDGGVGVLHT
                     IGREREAGLDPFISKYIFPGAYLPTIGQLGDSMAEAGLVMTDMENLRLHYARTLDSWA
                     ERFEARVEEIRDRFGERFVRMWRFYLHGCSSGFKWGDLRVFQITFTNGLNSALPMTRS
                     HVYVR"
     gene            3887..>4175
                     /locus_tag="VFD74_01030"
     CDS             3887..>4175
                     /locus_tag="VFD74_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HZK48169.1"
                     /translation="MRRPLKSRVHYAWLVLATSTLAVFGSLGLARFGYTVVLPSMQAG
                     LGFDNTQAGGLATANLIGYSALAVLGGALASRFGPRLVVTAGLITAGLAMLF"
BASE COUNT          568 a         1360 c         1496 g          751 t
ORIGIN      
        1 cccctcgtcc ttcctggtgg tggagcgaga acgtgagacc ctggcggcga tcgaccgagc
       61 catgggcggc gtcggccgcg tgggctgcgc ggacggcgtg gacggccggg cgatcggccg
      121 agcggatgtc ggtctgcggt cggcggggga ggtcgcggat acgcttgccc gtttggagcg
      181 ttcggaccgg agcggcgccc gggtgccgct gaccttcccc tctgaaccgt ggtcggcgcg
      241 gaccttcttc aacctggctc ccatactggc cgacgcggat tccggcgcga acgggttcgt
      301 gatcgcggtg catcccgact acgaggagct ggacctgggc tgcctgctgc ggccggagcc
      361 acgcacaggg gccgccgtac cctcgggcgc atcgcccctc ggggcttccg cactgcccct
      421 cgggtcgggc gcttcgccat gccctccctc gaggattctg ccctctgcgg ccctcgaagc
      481 cgtcgtctgg tcgtgggccc ttctgcaacg atgggcagac catcgcgcgc ttctctcgat
      541 ccccttcatc aacggcggga aggactccgc ctccggccag agcctggcgt gcttccacgc
      601 ccagttctat gccctggccg ccgacccgcc gcccccgtcg tacggttgtt cgcgtgggtg
      661 ggcggctgct gaggagtgtc cgttgtgcgc gctcagggac gtcgggggct tcgaggtgac
      721 ccgcgtcggg tcggtgggcg tgttcgccca tcccgcgccc gagatcgaac tgactttgct
      781 cgtggcctcg ctcggacacg cgggcgggat caccgacctg ccggatcccg ccgatcttgc
      841 gaaggccttg gcgctcgccg tgcgctccta cgagcacctg cttggcgggg tgccccccta
      901 caacatcgtt gtccgcaccg gagcgggggc gggtcacctg cacgcggagg tgatgccgcg
      961 ggccggagtg ccggtggcag ccgggtttga gaaggccacc ggactcttcg tctcgacccg
     1021 cgacccgcgg gtcttggccg agggcctgcg cgcctgggtc gagggccggg ctcccgctcc
     1081 gtgagcccgc ccgagtcacg cggccggggt cccgcccccg ggtcgcccgc cgccgggtcg
     1141 cccgcggcgg gtagactgcc ggcggagctc cacgcggcgc tccaacgcct ggcaggtctg
     1201 ccgggacgct ccgccctctt cttcgacgtc gacggcgtgc tggcgtccat ccgaccccgg
     1261 cctgaggagg cggcggttcc gcggaccgtg cggcgactgt tggtcgacct ggttcccgcg
     1321 tatcggctgg tggcggccgt gtcgggccgg agcctcgagg aggcggtcgc tatggtcgac
     1381 actccaggag tcgtggtcgt gggagaccac ggccgcgaga ccagggacga acacggccgc
     1441 gtcacgagga gcagggccgc cgaacgcgga ctcatccgtg aggcggccgc ggtcctcggc
     1501 ggcgacgcgg gcctccggcg ggcgggagtg cgcatcgagg agaagccggc cagcatcgcc
     1561 cttcatgtgc gcggcgccgc cgacatcgcc cgagctgaga cggcggctct tgaagcggcc
     1621 gcggccgtcg gacgacgctt cggactgacc gtgcggcaag gccgctcggt catcgacctc
     1681 aagattcccg gtgtggacaa ggggacggcc gtggaacgat tggtggcccg ctacggggtg
     1741 gagtcggtcc tctatgtagg cgacgacacc accgacgtcg acgccatgcg ggcgctggcg
     1801 cggatgcgct ccgacgtctt cttcgccgta ctgctgggtg tcagttcgaa tgagatgccg
     1861 gcggaactcg ccgcggcgtc cgatcatctg gtgcggcagc gggaggtggc gtcggtgttg
     1921 cgggcgctcg cgcctccgcg tgtgtgagtc gcccgccggg cttgtgacgc ctgcggcctg
     1981 ccgccgccgg tgtccgccga cgtccgggtg cgtgcgcgga tgttcgccga cttccggcga
     2041 cttccgccga tgtccggcgg cgtccggcta gtagcggccg acgagcttcc agatgccgcg
     2101 gtgcagtgcg tgcggcgaca gagtgggggc caggtacatc accgcgcacc cgccgctctc
     2161 cacggctgcg ccggcggccg cggccagttg cagagcttcc ggcgtgtcgg tcatctggtg
     2221 cagccagatg cgcttgaagc cacggtcgag ggcttccgat accgtgccgg ccgtcttctc
     2281 cttggccact tcgatgatga cggcctcggt gtcctcgggc acctcggcca gactgcccag
     2341 gaagccggcc tgtcctccgg acaagtcgac gggatacacc gtctttccct gttccatcag
     2401 gtacttgtgg gtgaagtgcg ggaacttctt gtcccgtgaa tcgccgacca gcgcgtaccg
     2461 ttggtgcgag aagaagtccg acttgcctga catgtgccct cctcaggggt ctctgtggcc
     2521 cgagcgatct cgacagctcg cgtcgtcgag caccgcttgc tgctgtcgct attgtgacgg
     2581 cttttcggcc gcgatgcacg agtcatctcc ttccacctct ggcggacggt ctgcttcgga
     2641 gttaggattg ccgtcacgaa gggttcatcc gggtcatgtg cgaggtgggc gctgtgatcg
     2701 gcaccgcaga cggcatattc cagaatcttg ccgcgggagt gagagactct gcgttccggg
     2761 tgcgcttctg ggacgggacc gagcgcacct acggggacgg ggacccgcgc ttcaccgtgg
     2821 ccttcgattc gcgggaggcg ctggacgcgg tgctcacgct cggatcgctc ggcacaggcg
     2881 aggcgtacat ggagggcgcg ctagatgtcg agggtgacct gcacgagttc ttccgcgtcg
     2941 gcagtcaccc gtctgtgcgg agtccgcggc tcggcactcg cgacaaggcc atgatagtgg
     3001 cggcgtacgt gcggaatcgc aacactctgc gcaaggcccg ggagaatatc gagcaccact
     3061 acgatctggg caacgacttc tatcgcctct ggctcgacga gggcatgacc tactcctgcg
     3121 cgtactggga tgagacctgc gacggcgacc tgggagcagc gcagcgggcc aagtacgagc
     3181 acatctgccg caagctgcac cttcgcgcgg gcgatcggct ggtcgaattc ggcagcggat
     3241 ggggtggaat gctcgcccat gccggccgcg agtacggcgt cgagggggtg gggtgcacgc
     3301 tctcgaccca gcagcacgag tacgcgagcg agcgattcgt cgccgagggg ctccatccac
     3361 gcgtacgttc ggagttgatc gactatcgga agatggaggg cacgttcgac aagttcgtgt
     3421 ccatagggat gttcgaacac gtgggcaggg agtactactc gacattcttt gagaaggtcc
     3481 gggccctgtt ggtggacgga ggggtgggcg tgctgcatac catcggcagg gagcgcgaag
     3541 ccggcttgga ccccttcatc tcgaagtaca tattccctgg cgcgtacctt ccgacgatcg
     3601 gccaactggg tgactccatg gccgaagctg ggctcgtgat gaccgacatg gaaaacctcc
     3661 ggctgcacta cgcgcgtacc ctcgactcct gggcggagcg cttcgaggct cgggtcgaag
     3721 agatcaggga taggttcggt gagcgattcg tccgcatgtg gcggttctac ctgcacggat
     3781 gctcgtccgg cttcaagtgg ggggatctca gagtgttcca gatcacgttc acgaacgggt
     3841 tgaactcggc gctgccgatg acccgatcgc acgtgtacgt gcgttgatgc gccggccgct
     3901 caagtcacgg gtgcactacg cctggctcgt cttggcgact tccacgctgg ccgtgttcgg
     3961 atctctggga ctggcgcgtt tcggctacac ggtcgtgctc ccctccatgc aggcgggcct
     4021 gggtttcgac aacacgcagg cgggaggctt ggcgacggcc aacctcatcg gctactcggc
     4081 cttggccgtg ctgggtggag cgttggcgtc gcgtttcggg ccacgcctcg tggtcaccgc
     4141 ggggttgatc acggccgggc tggccatgct gttca
//
LOCUS       DASRMJ010000035         7401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_77814, whole genome shotgun sequence.
ACCESSION   DASRMJ010000035 DASRMJ010000000
VERSION     DASRMJ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77814"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFD74_01035"
     CDS             <1..196
                     /locus_tag="VFD74_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZK48170.1"
                     /translation="MFKHVVKQVLACGIPEDRIILSLERRMKCGVGECGHCQINGVYV
                     CQDGPVFTYAELKRLEEAML"
     gene            196..996
                     /locus_tag="VFD74_01040"
     CDS             196..996
                     /locus_tag="VFD74_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HZK48171.1"
                     /translation="MREPMIEALQTLKRPRVAVFELASCAGCQLQILNCEDELVDLLQ
                     LVEFAYFKEAMTDTSDVYDLALIEGAVTREADEEKLRTIRERAKLVGTLGACAFPVGI
                     PGLKNTHDLDEIGRHVYGEQAGWFPSVRAKPVDAVIHVDLRIPGCPIDKDEFLRVVTG
                     VLLGCRPPIPGYPVCVECRLKENVCAYDRGLVCLGPVTRAGCGAICPSYGSRCFGCRG
                     LADDPNVPSVENLLEERGLTRDDILAEYALYNGYYALNDGGLKAKVAT"
     gene            993..2318
                     /locus_tag="VFD74_01045"
     CDS             993..2318
                     /locus_tag="VFD74_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HZK48172.1"
                     /translation="MSKTIAVDVKVDYVSRVEGHGNIVVVAQDGQVKEVRFEVTEPPR
                     FFEGMLRGRPYEQAPLITSRICGICSNGHVLASITAMESALGISPSDQTKLLRRLLLH
                     GETIQSHVLHAYFLVAPDFFGVGSVIPLVETHPDVVKRALRLKKLGNDICDVVGGRAV
                     HQIAATVGGFTHLPSPDELRALRGRLADAAPDLEATVSLFAGLAMPEFDHATEYLSLK
                     SAVGYAYFGDLILSSNGDITPVHHYREKVKERVVPHSSAKHCASNKDTFMVGALARAN
                     NGFQHLSDGAKDAAAALGFHAPATNPYLNTVAQLIETVHCVDDACAIIDTLLAGGLTP
                     EPVDVEVCEGHGVGAVEVPRGTLYHEYAIDRQGHITDANLIIPTGQNLANLESDMWAL
                     APAVIPKGADAAQLAMEMLVRAYDPCISCSTHVIYLDAGGAAAETEVSA"
     gene            2315..2770
                     /locus_tag="VFD74_01050"
     CDS             2315..2770
                     /locus_tag="VFD74_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZK48173.1"
                     /translation="MSVNRTLVAENELLSDLDPVDRDALVAVAIGKTYAAGDVIFKEN
                     EDAYAVCLLLEGRIGLQMDVGNGRNLMVSTVDAGEVFAWSGLVRPHQFTATARAIEDC
                     EVAIFKSEDLTRLFQENPRFGYAVMLRVASLIGERLRDTHLQLLGMFGA"
     gene            complement(2826..4067)
                     /locus_tag="VFD74_01055"
     CDS             complement(2826..4067)
                     /locus_tag="VFD74_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HZK48174.1"
                     /translation="MDISGLRVNGQRLQDSLEQMAKIGATPGGGVQRLTLSDEDKHAR
                     DLFISWLKEIDLEVTVDEMGNIFGRRPGKNNDLPPVFSGSHIDSQPKGGRFDGILGVM
                     GALEVMRTLHDNDIETERPVLIVDWTNEEGSRFSPAMVASGVWAGKLDRDYAYSRTDK
                     DGKVFGEELERIGYKGTVPAKAWPFHAYYELHIEQGPILEREGKTIGIPKGILCLHWY
                     DVYLEGKANQVGPTPMEGRNDALVAAAEMILAVQSVPGRVGGNLVATVGEIQNYPNSR
                     NIIPDGVHFTVDIRSWDDDLAIQAWEQLKLDFQAAADKNGCPMRLEETWRVEHTPFAQ
                     KLVDFLAQKADALGYSAHHMVSGAGHDASYMNMLGDAAMIFIPSIGGRSHVEVENSTW
                     EDCTAGANALLHCMLESANEV"
     gene            complement(4190..4432)
                     /locus_tag="VFD74_01060"
     CDS             complement(4190..4432)
                     /locus_tag="VFD74_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48175.1"
                     /translation="MEESRRVLELEDRLAEVEHALNGISARGGSAARYLRREIEDLLE
                     ECRTQRDRWAEIARRAEHTLSVLDETRERQEGRLEA"
     gene            4569..5012
                     /locus_tag="VFD74_01065"
     CDS             4569..5012
                     /locus_tag="VFD74_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HZK48176.1"
                     /translation="MKFLIVYGSIGGNTEKMAGFIAEGIRIGGAEADVKPFSDVREPA
                     DFAGYDGYMFGGPTYHKDLLHGMKAWLFKAKEGGLESKVGGAFGSHTHSGEAPGILFD
                     TMLHVFKMNMADLGPFKMEESVIGTPAGIKACQQYGKALLDRASA"
     gene            5143..5316
                     /locus_tag="VFD74_01070"
     CDS             5143..5316
                     /locus_tag="VFD74_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48177.1"
                     /translation="MRVVVAARHGGPETLRVEERPDQVPGPGEVRVRLEEAAEAHRAV
                     KSRSAIGKVMLAA"
     gene            5377..6432
                     /locus_tag="VFD74_01075"
     CDS             5377..6432
                     /locus_tag="VFD74_01075"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZK48178.1"
                     /translation="MDGIETFIRAMPKVELHLHLEGTLETEHMFRLAARNGVALRHTS
                     VEAAHAARDFRDLQSFLHEYYEGASVLREEIDFYELTQEYMARAHANGIRHAEVFFDP
                     QTHTARGVSFEAVVSGIHRALGEARSELGITSRLIMCFLRDRSIGSAEATLDAALGQG
                     DLIAGVGLDSAEVGHHPGDFLSVFARARKAGLHAVAHAGEEGPPAYISEALDLLGAER
                     IDHGVRCLEDEDLVARLVAERVPLTVCPLSNVRLRVFASMEQHNLKRLLDRGLVATVN
                     SDDPAYFGGYLTDNFISAQRALGLDRRDVLTLALNAVEAAFVDDERREQLRDELMCFW
                     RCGPTDGSLGHSRSARG"
     gene            6478..>7401
                     /locus_tag="VFD74_01080"
     CDS             6478..>7401
                     /locus_tag="VFD74_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HZK48179.1"
                     /translation="MGHGAHLSEEYRLLSKRLQRGPAAMPEPVDATARAAWREILEIL
                     FTPEEAALAAKMPVAPTPISALVARAGMTEAELVSRLDAMAEKGVVMDMPGREGREPQ
                     YMLAPPVVGFFEFSMMRAKDSIPKKRMAEALHAYTLGDDTFAREIFSTPTVVGRTVVH
                     ETVMPQPDILENNLPEVLDWERASSLVGDSKRLAVSLCYCRHKAQHLGHACENPADIC
                     LTLEGGADYVVRHGFGREIDRAEGLELVAKARELGLVHVVDNVRNRPTYLCSCCGCCC
                     GQLSAIRRFDLPAVVPSGFEARPATETCTGCG"
BASE COUNT         1243 a         2315 c         2507 g         1336 t
ORIGIN      
        1 gatgttcaag cacgtagtga agcaggtgct cgcctgcggc atacccgagg accgcatcat
       61 cctgtccctg gagcggcgga tgaagtgtgg ggtgggggag tgcggtcact gccagatcaa
      121 cggggtgtac gtctgtcagg acgggcccgt cttcacctac gctgaactca agcggctgga
      181 ggaggcgatg ctgtgatgcg cgaaccgatg atcgaagccc tccagacgct caagcgacct
      241 cgggtggcgg tgttcgagct tgcctcgtgc gccgggtgtc agttgcagat actgaactgt
      301 gaggatgagc tcgtggacct cctccaactg gtcgagttcg cctacttcaa ggaagcgatg
      361 accgatacgt cggatgtcta cgaccttgcc ctcatcgagg gtgcggtgac ccgggaggcc
      421 gacgaggaga agctccgcac catccgggag agagccaagc tggtggggac actgggagcc
      481 tgcgccttcc cggtgggcat ccccgggctc aagaacacgc acgacctcga cgagatcgga
      541 cggcacgtgt atggagaaca ggcgggatgg ttcccctccg tcagggccaa gccggtggac
      601 gccgtcatcc acgtggacct gcgcatcccc gggtgcccga tcgacaaaga cgagttcttg
      661 cgggtggtca cgggcgtgct gctcgggtgc aggccgccca taccgggata tccggtgtgc
      721 gtcgaatgcc ggctgaagga gaacgtgtgc gcctacgatc gcggcctcgt ctgcttgggt
      781 ccggtcaccc gggcggggtg cggcgccatc tgccccagct acggaagtcg ctgcttcggc
      841 tgccgtggcc tggccgatga cccgaacgtg ccctcggtgg agaacctgct ggaggagcgc
      901 ggtctcaccc gtgacgacat cctggccgag tatgccctct acaacggcta ctacgccctg
      961 aacgacggtg ggctgaaagc gaaggtggcc acgtgagcaa gaccatcgcc gtcgacgtca
     1021 aggtcgacta tgtgtcccgg gtcgaggggc acggcaacat cgtcgtggtc gcgcaggacg
     1081 gccaggtgaa agaggtgcgg ttcgaggtga ccgaaccgcc gcgcttcttc gaagggatgc
     1141 tgcgcggacg gccctacgag caggcgccgt tgatcacatc gcgcatctgc ggcatctgtt
     1201 ccaacggcca cgtactcgcc tccatcaccg ccatggagag cgccctcggc atctcgccct
     1261 ccgaccagac caaactgctc cggcgacttc ttctgcatgg agagactatc cagagccacg
     1321 tcctgcatgc ctacttcctc gtggcccccg atttcttcgg ggtggggagc gtgatcccgt
     1381 tggtggagac ccaccccgat gtcgtgaagc gggccctgcg cctcaagaag ctcggcaacg
     1441 acatctgtga cgtggtcggc ggccgggccg tgcaccagat cgccgcaacg gtgggaggat
     1501 tcacgcatct gccgagcccg gacgagctgc gagcgctgcg tggtcgcctg gccgacgccg
     1561 cccccgatct cgaggccacc gtgagcctct tcgcggggtt ggcgatgccc gagttcgatc
     1621 acgccaccga gtacctctcg ctgaagtcgg ccgtcggcta cgcgtacttc ggcgacctga
     1681 tcctctcgtc caacggggac atcacccccg tgcaccacta tcgggagaag gtcaaggagc
     1741 gggtggtgcc gcactcctcg gccaagcact gcgcctcgaa caaggatacg ttcatggtgg
     1801 gcgcccttgc ccgcgccaac aacggtttcc agcacctgtc cgacggggcg aaggacgcgg
     1861 ccgctgcgct gggcttccac gcgcccgcca cgaaccccta cctgaacacg gtggcacaac
     1921 tcatcgagac ggtgcactgc gtcgacgacg cctgcgccat catcgacacg ctgctcgccg
     1981 gcggactcac ccctgagccc gtggatgtgg aggtgtgcga aggtcacggg gtgggggcgg
     2041 tcgaagtgcc tcgcggcact ctctaccacg agtacgcgat cgatcgacag ggacacatca
     2101 ccgacgccaa cctcatcatc cccaccggcc agaacctcgc taatctggag agcgacatgt
     2161 gggccctcgc ccccgcagtg atacccaagg gcgccgacgc cgcgcagctc gccatggaga
     2221 tgttggtgcg ggcctacgac ccgtgcatat cgtgctccac gcacgtgatc tacctcgacg
     2281 caggcggtgc ggctgcagaa acggaggtat cggcatgagc gtgaaccgca ctctggtcgc
     2341 cgagaacgag ctcctgtcgg acctcgaccc ggtcgatcga gacgctctgg tagctgtggc
     2401 tatcggcaag acctacgccg cgggcgacgt gatcttcaag gagaacgaag atgcctacgc
     2461 ggtgtgtctg ctcctcgaag gtcgcatcgg gctccagatg gatgttggga acggtcgcaa
     2521 cttgatggtg agcacggtgg acgcgggtga ggtcttcgcg tggtcgggat tggtgcgtcc
     2581 ccaccagttc accgccactg cccgggccat agaagactgc gaggtcgcca tcttcaagtc
     2641 cgaggatctg acccggttgt ttcaggagaa tccgcggttc ggctacgcgg tcatgttgcg
     2701 cgtcgcttcg ttgatcggtg aacgtctacg cgacacccac ctgcagctgc tcgggatgtt
     2761 cggagcgtag cgcatgggga ggcgggcgcc gcgcgcggcg cccgcctcga ccggaagtcc
     2821 ggcgactaca cttcgttggc ggattccagc atgcagtgga gcagggcgtt cgcgcccgcc
     2881 gtgcagtctt cccaagtgct gttctcaacc tcgacgtggc ttcgtccgcc gatgctgggg
     2941 atgaagatca tggctgcgtc acccagcatg ttcatgtagc tggcgtcgtg accggcgccg
     3001 ctgaccatgt ggtgggcgga gtagccgagc gcgtccgctt tctgagccag gaagtcgacg
     3061 agcttctgtg cgaacggagt gtgttcgacc cgccaagtct cctcgaggcg catggggcag
     3121 ccgttcttgt cggcggcggc ctggaagtcg agtttcagct gttcccacgc ttggatagcg
     3181 aggtcgtcgt cccacgagcg gatgtccacg gtaaagtgca cgccgtcggg gatgatgttg
     3241 cgcgagttcg ggtagttctg gatctcgccg acggtggcga ccaggttgcc gcccacgcga
     3301 cccggcaccg actgcacggc caggatcatc tcggcggccg ccaccagggc gtcgttgcgg
     3361 ccctccatcg gcgtcgggcc cacttgattg gccttgcctt cgaggtacac gtcataccag
     3421 tggaggcaga ggatgccctt ggggatgccg atggtcttgc cttcacgctc caggatgggt
     3481 ccctgctcga tgtggagttc gtagtaggcg tggaagggcc aggccttcgc cggcacggtg
     3541 cccttgtagc cgatcctctc gagctcctcg ccgaacacct tgccgtcctt gtccgtgcgg
     3601 gagtaggcgt aatcacggtc gagtttgcct gcccacacgc ccgaggcgac catggcgggt
     3661 gagaagcgcg atccctcttc gttggtccag tcgactatca ggacggggcg ttcagtctcg
     3721 atgtcgttgt cgtgcagggt gcgcatcacc tcgagagctc ccatcacgcc caggatgccg
     3781 tcgaagcgtc cgcccttggg ctgcgaatcg atgtgcgaac cgctgaagac gggcggcaga
     3841 tcgttgttct tgcccgggcg acggccgaag atgttgccca tctcgtcgac ggtcacttcg
     3901 aggtcgatct ccttgagcca gctgatgaag aggtcgcgag catgcttatc ctcatcggaa
     3961 agggtgagcc tctgcacgcc gcctccgggc gtcgcaccta tcttcgccat ctgctccagg
     4021 ctgtcttgga ggcgctgacc gttgacccgc aatccactga tatccatgcg tacctgctcc
     4081 gcgtctgagg ttctccatcg acctttctac gctaccggag tcgacggacg acggcaagtg
     4141 ggttggggcg cacttccctg tgagaccggc cggcggcgcg ccggccggtc tatgcctcaa
     4201 ggcgcccctc ttggcgttcg cgtgtctcgt ccaagaccga gagcgtgtgc tccgcccgcc
     4261 gcgcgatctc cgcccaccga tcccgctgcg tgcggcactc ctccagcagg tcctcgatct
     4321 ctcggcggag atagcgggct gcgctgccgc cgcgcgctga gatgccgttc agcgcgtgct
     4381 ccacctccgc gaggcgatct tccagttcca gaactctgcg tgattcttcc atgtggctcc
     4441 tcgatcttgc gtgcggtggc gggtgcggca gcggtagtct tcggtttgag cctcgcccgc
     4501 ccgcgggtag gccgagcccg gcgcgacggc gcaccaggat caaccttatc gcagcagagg
     4561 agcgtgagat gaagttcttg atagtctacg gcagcatcgg cggcaacacc gagaagatgg
     4621 ccgggttcat agcggaaggc atcaggatcg ggggcgccga ggcggacgtg aagccgttct
     4681 cggacgtgcg cgagcccgcg gacttcgccg gctacgacgg ctacatgttc ggtgggccca
     4741 cctatcacaa ggatcttctc cacggtatga aggcctggct cttcaaggcc aaggaaggcg
     4801 gcctcgagag caaggtgggc ggggctttcg gctcacacac acactccggc gaagcccctg
     4861 gcatcctgtt cgacacgatg ctacacgtgt tcaagatgaa catggccgac ctcggtcctt
     4921 tcaagatgga ggagtcggtg atcggcacgc cggccggcat caaggcctgc caacagtatg
     4981 ggaaggcgct tctggataga gcttcagcct agagacccgt cacccgtgcc cggcgtgaat
     5041 gcccactcgc caccacgcgg gacgtggggt gctcgatgtt gacgatgccg cctctgtggg
     5101 aacaagcggc ccaatcatcg agatggtggt gaggagggac cgatgagggt cgtggtcgcg
     5161 gcgcggcacg gaggaccgga gacccttcgg gtggaggagc gtcccgacca ggtgccgggt
     5221 cccggtgagg tgcgcgtgcg gttggaggag gcagccgaag cccatagggc tgtcaagagc
     5281 cgctccgcca tcggcaaggt catgctggcg gcgtgaggag tgtcgatcgt ggggggatcc
     5341 gaccggagcg gggagagcgg cgctgccgga gatcagatgg atggtatcga gacgttcatc
     5401 agggcgatgc cgaaggtgga gttgcatctg cacctcgaag gcactctcga aaccgagcac
     5461 atgttccgtc tggccgcgcg caacggcgtg gccctgcggc acacgtcggt ggaagccgcc
     5521 cacgccgccc gcgatttccg cgacctgcag tcgttcctgc atgagtacta cgaaggcgcc
     5581 tcggtgctac gcgaggagat cgatttctac gagctgaccc aggagtacat ggcgcgggcc
     5641 catgctaacg gtatccggca cgcagaggtg ttcttcgatc cgcaaaccca caccgcccgc
     5701 ggggtctcct tcgaggcggt ggtgtccggc atccatcggg ctctcgggga ggcgcgatca
     5761 gaactcggca tcacgagccg tctcatcatg tgcttcctca gagaccggag tatcggctcc
     5821 gcggaggcga cgctcgatgc cgcactcggg caaggtgatc tgatcgcggg ggtgggcctg
     5881 gattcagctg aagtcggcca ccatcccggg gacttcctca gcgtgttcgc tcgggcgcgc
     5941 aaggccgggc tgcacgcggt ggcacacgcc ggcgaggagg ggccgcccgc gtacatatcg
     6001 gaagccctcg atcttctggg tgccgaacgc attgatcacg gagtccgctg cctcgaagac
     6061 gaagacctgg tggcccggct ggtggcggag cgtgtgcctc tgacggtctg cccgctttcg
     6121 aacgtgcgac tccgcgtctt tgcctcgatg gagcagcaca acctgaagag gttgttggac
     6181 cggggtctgg tcgcgactgt caattcggac gatccggcct acttcggagg ctacctcacc
     6241 gacaacttca tctccgcgca gagagccttg gggttggaca ggcgcgacgt gctgacactg
     6301 gcgttgaacg ctgtcgaggc tgcgttcgtc gacgacgagc gtcgcgagca gctgagggac
     6361 gaactcatgt gcttctggag gtgtggcccc acagacggtt ctttgggcca ctcccggtcc
     6421 gcgcgtggct gactgctatt ctgtagcgga aacccaggct cggagaaggg ggttgccgtg
     6481 ggccacggcg cacacctgag tgaagagtac cgactgctct cgaagcgcct gcagcgcggg
     6541 ccggcggcca tgccggagcc cgtcgacgcg acggcgcggg ccgcctggcg tgagatcctt
     6601 gagatactgt tcacgccgga agaagcggcg ctcgccgcga agatgcccgt ggcacccacg
     6661 cccatcagcg cgctcgtcgc ccgcgcgggg atgaccgagg cggagctggt ctcccgtttg
     6721 gacgccatgg ctgagaaggg cgtcgtgatg gacatgcccg gccgagaggg gcgggagccg
     6781 cagtacatgt tggcgccgcc ggtggtgggg ttcttcgagt tttccatgat gcgcgcaaag
     6841 gattccatcc ccaagaagcg tatggccgag gcgcttcacg cctatacgct cggcgacgac
     6901 accttcgcgc gggagatctt cagcactccc accgtggtcg gccggaccgt agtgcatgag
     6961 acggtcatgc cgcaacccga cattcttgag aacaaccttc ccgaggtgct cgattgggag
     7021 agggcttcca gtctcgtcgg ggactcgaaa aggttggcgg tctcactctg ctactgccgc
     7081 cacaaggctc agcacctggg gcacgcctgc gagaatccgg ccgacatctg cctgactctg
     7141 gagggcggag ccgactacgt ggtgcgacac ggcttcggcc gcgagatcga ccgggcggag
     7201 gggctggagt tggtggcgaa ggcacgggaa ctcgggttgg tgcatgtggt ggacaacgtg
     7261 cgcaatcggc ccacctatct gtgcagctgc tgcgggtgct gctgcggcca gttgtcggcc
     7321 atcaggcgct tcgaccttcc ggcggtggtg ccgagcggct tcgaggcgcg tccggcgacc
     7381 gagacctgca cgggttgtgg c
//
LOCUS       DASRMJ010000036         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_402479, whole genome shotgun sequence.
ACCESSION   DASRMJ010000036 DASRMJ010000000
VERSION     DASRMJ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402479"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFD74_01085"
     CDS             <1..455
                     /locus_tag="VFD74_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017810984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HZK48180.1"
                     /translation="FVVGTDVLRPRLVGTEDSLGVRVPDHAALLQLLDAIGIPLAATS
                     ANRSGRPDVAEAADVAPGLLTVCAAAFAGGAAAAGEASPGEEGSGRGMTSTHGVASTV
                     VDLRPLATGGSALVLREGAVPAAEALRRVEAAQAGRAPHAGSRSAPTG"
     gene            592..768
                     /locus_tag="VFD74_01090"
     CDS             592..768
                     /locus_tag="VFD74_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48181.1"
                     /translation="MENEHRDDPSQTGGHSVGTYLEETKISAGKHGRWFYLIWVVVWL
                     VLLAFLLVIYSLLT"
     gene            911..1354
                     /gene="rpiB"
                     /locus_tag="VFD74_01095"
     CDS             911..1354
                     /gene="rpiB"
                     /locus_tag="VFD74_01095"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZK48182.1"
                     /translation="MRYAVGCDHAGLPLKAPLVAELERQGHEITDFGTDGPESVDYPL
                     FAARVARAARDGDADMGLLLCGTGLGMAMTANRFRGVRAASVSESFSARMARAHNDAN
                     VLCMGARVVGPGAAVEILKAWLEQGFEGGRHQRRVDMVMHPDGND"
     gene            1463..>2117
                     /locus_tag="VFD74_01100"
     CDS             1463..>2117
                     /locus_tag="VFD74_01100"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZK48183.1"
                     /translation="MAQVDPVIAALVDRELRRQQENIELIASENFTPIAVLQAVGSVL
                     TNKYAEGLPGKRYYGGCEVVDQVETLAIERAKDLFGAEHANVQPHSGSQANHAAYVSA
                     LEHGDVVLAMRLEHGGHLTHGMKINFSGREYVFHHYGVREDTGRIDYDQVREMARTFR
                     PKLIVAGASAYPREIDFAAFRQIADEVGAQLMVDMAHIAGLVAAGAHASPFGLAEWVT
                     "
BASE COUNT          344 a          672 c          747 g          354 t
ORIGIN      
        1 cctttgtggt gggcacggac gttctgcgac cgcggctcgt gggcaccgag gactccctcg
       61 gtgtccgggt gcccgaccac gcggcgctac ttcagttact cgatgccatc ggtatccctc
      121 tggcggccac gagtgcgaac cggagcggga gacccgacgt agccgaggcc gccgacgtgg
      181 cccccggtct cctcaccgtg tgcgccgcgg cgttcgccgg cggcgccgct gcagccgggg
      241 aggcctcccc tggggaggaa ggctctggta gggggatgac ctcgacacac ggagttgcct
      301 ccacggtcgt cgacctgcgc cccctggcca cgggtggcag cgctctggtg ttgagagaag
      361 gcgcggtgcc ggcggcggag gccctgagac gcgtggaggc ggcacaggcg ggtcgcgctc
      421 cgcacgcggg gagtcgatcg gcgcctaccg ggtaagtgat acacgctatt gtctcaggcg
      481 tactctgggt acccttcgtt tgtgatgggc cgccacagcg tgtgagaggg caccgagggc
      541 cccctagccg gccgctgtac cgagatgtca acgagcagaa agggcagtga catggagaat
      601 gagcaccgcg acgatccttc gcagaccgga ggccattccg tcggcacgta cttggaagag
      661 accaagatat cggcgggcaa gcacggccgt tggttctacc tgatttgggt cgtggtctgg
      721 ttggtgctcc tagccttcct gctcgtcatc tattcgctcc tcacctgagg gactcggcga
      781 gatcgcacct ctccggcgca cggggggcat cgatcccggc acaaacggct ggggcgggag
      841 cctcgagggg cgccgtcggt agggtccttt ctgctatgct ctcccccggt atcgaacggg
      901 gaagaggttc atgcgctacg ccgtcggttg tgatcatgcc ggcctacctt tgaaggctcc
      961 tctcgtggcc gagctggagc gtcagggtca cgagatcaca gacttcggca cggacggtcc
     1021 ggagagcgtc gactacccat tgttcgccgc ccgcgtggcg cgggcggcgc gcgatggtga
     1081 cgccgatatg gggctactgc tctgcggcac cggcctcggg atggccatga ccgcgaatcg
     1141 cttccggggg gtccgcgctg cgagcgtgag cgagtctttc tcggcgcgta tggcccgcgc
     1201 ccacaatgac gccaacgtgc tctgtatggg cgcccgcgtc gtcggtcccg gggcggcggt
     1261 cgagatcctc aaggcttggc tggaacaggg gttcgaaggc ggccggcatc agcggcgcgt
     1321 ggacatggtc atgcaccccg acggaaacga ctaatcgaca tacgacgagc aggcggcgcc
     1381 ggaccagtgg cggccgttcc gcgactggag gaaggctgag gtggatcacg tcccccccga
     1441 ggcggggtat ttcgagcaga gtctggcgca ggtagatcct gtgattgccg ctctggtgga
     1501 ccgggagctt cggcggcagc aagagaacat cgagctcata gcctcggaga acttcactcc
     1561 gatcgcggtg ctgcaggcgg tgggcagcgt gctcaccaac aagtacgccg agggtctgcc
     1621 cggcaagcgc tactacggcg gttgcgaggt cgtggaccag gtggagacgc tggccatcga
     1681 gcgggccaag gacctgttcg gagccgagca tgcgaacgtg cagccccact ccgggtctca
     1741 agccaaccac gccgcctacg tatccgcgct cgagcacggc gacgtcgtct tggccatgcg
     1801 actcgagcac ggcggacacc tcacgcacgg catgaagatc aacttctcgg gccgcgagta
     1861 cgttttccac cactacggcg tgcgcgagga caccggccgc atcgactacg accaggtgcg
     1921 ggagatggcc cgcacgttca ggccgaagct gatcgtggcc ggagcgagtg cgtatccccg
     1981 cgagatcgat ttcgccgcct tcaggcagat cgccgacgag gtcggggcgc aactcatggt
     2041 cgacatggcc cacatcgccg gcctcgtggc ggccggggcg catgcatctc ccttcggact
     2101 ggccgagtgg gtcacca
//
LOCUS       DASRMJ010000037         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_473215, whole genome shotgun sequence.
ACCESSION   DASRMJ010000037 DASRMJ010000000
VERSION     DASRMJ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473215"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFD74_01105"
     CDS             <1..668
                     /locus_tag="VFD74_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZK48184.1"
                     /translation="TRGLPVLRRGPSDNRLYRAVWLAIYAPVRLLFRMRISGLEHIPA
                     HGKVVLACNHISNLDPIYLGAAADRQAHFMAKSSLWAFGPLGRLVAALGAFPVRRGEA
                     DREAIRAAISYLDVGAVVGIFPEGHRQLEGRLGDPQPGFALLALRKDVVTIPAVLTGT
                     DRIGRSGLPRLSKVTVTFGPPLDTEIGGTAKGERHDEMGRRLKDAWSVLLGRASGGEI
                     GEA"
     gene            665..1513
                     /gene="ispH"
                     /locus_tag="VFD74_01110"
     CDS             665..1513
                     /gene="ispH"
                     /locus_tag="VFD74_01110"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006722679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZK48185.1"
                     /translation="MSPFRVTVASSAGFCWGVERALRKARDAVETAKGPIETLGPLIH
                     NPGVIADLASQGIGLVDDVDTKTEGTVIIRSHGVPRGVMESLAAGDVAVVDATCSFVK
                     SAQTRAARLHDEGYRVIILGEPDHPEVLGIRSYAGPDAVVVEGPEDLPDDLDGRRVGV
                     VVQTTQSQARLAAITAALVTRVRELRVCNTICSATERRQEAAVAMAADADVVVVVGGR
                     TSGNTKRLVELCAQVQPHTHHIERAEEIDPAWLDGAAVVGVTAGASTPATQIESVVER
                     LRELGS"
     gene            1510..2835
                     /gene="der"
                     /locus_tag="VFD74_01115"
     CDS             1510..2835
                     /gene="der"
                     /locus_tag="VFD74_01115"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZK48186.1"
                     /translation="MSLASALPRVAVVGYPNVGKSTLFNRLTGSREAVVSPMAGVTRD
                     RKECPAEWLERRFVVVDTGGIDLKAKEPLSDQVREQARQAIALSAAVVFVVDGRSGVG
                     PQEHEIAEILRRGTVPVVLAVNKVDNREAAKRENEFWELGLGTPYGISAEHGLGIGDL
                     LDAVIEVLPPAELIAETAKPVNVAIVGRPNVGKSSILNALLGDERTIVSAIPGTTRDS
                     IDTPIDFEGREMNLVDTAGLRRRGKRSEEDVEFYSSLRTIRALERSDVALVVVDASEG
                     LVDLDLQVAYEAQRARCATAILFNKWDASTVDLDMTTARLNAKVQMRPPWVTISALTG
                     RNLTRVLPLALDLHDKYAARIPTGALNRWLEEVRGRKDPPQKRGKSLKLFYAVQYEQS
                     PPRFKVMVNSKALITRSYGYFLENRLRDAFSLWGIPLVIDFEGKEERYS"
     gene            2832..>3144
                     /locus_tag="VFD74_01120"
     CDS             2832..>3144
                     /locus_tag="VFD74_01120"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HZK48187.1"
                     /translation="MTEALLIVGAYLVGSISPSVFLGRLVRGIDLREHGSGNAGTTNA
                     FRVLGTGLGLAVLVADVAKGFVAALTASLLVGPTATVVVALAVMAGHNWSIFLRGKGG
                     KG"
BASE COUNT          541 a          939 c         1112 g          552 t
ORIGIN      
        1 gcacgcgagg actccccgtg ctgaggaggg ggccgtccga caatcgcctg tatcgagccg
       61 tctggctggc gatctacgcc cccgtcaggc tactattccg gatgcgcatc tcaggcctcg
      121 agcacatccc ggctcacgga aaggtcgtcc tggcctgcaa ccatatctcc aacctcgatc
      181 cgatctactt gggggcggca gcggaccggc aggcgcactt catggcgaag tcgagcctgt
      241 gggctttcgg tcccctcggc cgactggtgg cggctttggg agcgttcccg gtacgtaggg
      301 gggaggcgga tagggaggcc atccgggcgg ctatctcgta ccttgacgtg ggcgctgtgg
      361 tcggcatctt ccccgagggg caccgacagc tcgaagggcg tctcggcgac ccccaacccg
      421 ggttcgccct gttggcgctg cgcaaggacg tggtcacgat cccagctgtg ctcaccggca
      481 ccgaccgcat cggccgtagc gggctgcctc gtctgagcaa ggtcacggtc acgttcgggc
      541 cgccgctcga caccgagatc ggcggcaccg ccaagggaga gcgtcacgat gagatggggc
      601 ggagactgaa ggatgcgtgg agtgtcctgc tcggacgggc ctcgggtggc gagatcgggg
      661 aggcatgagt ccttttcgcg tgacggtggc ctcctcggcc ggattctgtt ggggggtgga
      721 gcgcgccctc cggaaggcgc gcgacgcggt ggagacagcg aaggggccga tcgagacgct
      781 cggccctctg atccacaatc ccggtgtgat agccgacctt gcgagtcaag ggatcggtct
      841 cgtggatgac gtcgacacta agaccgaggg aaccgtgatc atccgctcgc acggcgtgcc
      901 tcgcggcgtc atggagtcgt tggcggcggg tgacgtcgcg gtcgtggatg ccacctgctc
      961 cttcgtgaag tcggctcaga cgagggcggc ccggctacac gacgagggat acagggtcat
     1021 catcctcggg gagcccgacc accccgaggt gctgggcata cgctcctacg ccgggccgga
     1081 cgcggtggtg gtggagggtc cggaagatct gcctgacgac ctggatggtc gacgagtggg
     1141 cgtcgtggtg cagacgacgc agtcccaggc gaggttggcg gcaatcacgg cggcgctggt
     1201 gacacgggtc agggagctcc gcgtgtgcaa cactatctgc agtgcgacag agcgtcgaca
     1261 ggaggccgcg gttgcgatgg cggccgacgc cgacgtcgtc gtggtagtgg gcgggcgaac
     1321 gtcggggaac accaagcgtc tggtcgagtt gtgcgcgcag gtgcagccgc atacgcacca
     1381 catcgagcgc gctgaggaga tcgaccccgc ctggctcgac ggggcggcgg ttgtgggagt
     1441 gacggccgga gcatcgacgc ccgccactca gatcgagagc gtcgtcgaac gattgaggga
     1501 gctgggatcg tgagtctagc gagcgctctt ccgcgcgtgg ctgtagtggg atatccgaat
     1561 gtgggcaagt ccaccctctt caaccgtctc acgggttctc gtgaggccgt cgtctccccc
     1621 atggcggggg tgacgcgcga ccgaaaggag tgtccggcgg agtggctgga gcgacgcttc
     1681 gttgtggtgg acacgggcgg catcgacctg aaggcaaagg agccgctctc cgatcaggtg
     1741 cgggagcagg cgcgccaggc gatcgccctc tcggccgccg tggtcttcgt cgtcgatggg
     1801 cggagcgggg tgggcccgca agagcacgag atcgccgaga tcctccgccg gggaactgtg
     1861 cctgtggttc tggctgtgaa caaggtcgac aatcgagagg cggcgaagag ggagaacgag
     1921 ttctgggaac tcgggctggg cacgccgtac ggcatttcgg cggaacacgg cctcggtatc
     1981 ggagacctac tcgacgcggt tatcgaagtc ttgcctcccg cggaactcat cgccgagact
     2041 gccaagcccg tgaatgtggc catcgtcggg cgtccgaacg tcggcaagag ttccatattg
     2101 aacgcccttc ttggcgatga gcgtacgatc gtgtcggcta taccggggac tacccgcgac
     2161 tcgatagaca cgcccataga cttcgagggg cgcgagatga acctcgtgga cacggcggga
     2221 ctgcgtcgcc gagggaagcg cagcgaggaa gatgtggaat tctacagttc cctccgtacg
     2281 attcgggcgc tggaacggag tgacgtggcc ttggtggtgg tcgatgcatc cgaagggctg
     2341 gtcgacctcg acctacaggt ggcctacgaa gcgcaacggg cgcgttgtgc cacggccatc
     2401 ctcttcaaca agtgggacgc atcgacggtc gacctcgaca tgacgacggc ccgcctgaac
     2461 gcgaaagtgc agatgaggcc cccgtgggtc accatctcgg cgctcacggg ccgcaacctc
     2521 acccgcgtac ttcctctggc cctcgatctt cacgacaagt atgcggcacg catccccacc
     2581 ggcgctctca accgctggct cgaggaggtg cggggtcgca aagatccgcc tcagaagcga
     2641 ggcaagtcgt tgaagttgtt ctacgccgtg cagtacgaac agtctccgcc acgattcaag
     2701 gtgatggtga actcgaaggc gctcatcaca cgcagttacg gctacttcct tgagaatcga
     2761 ctcagggacg cgttctcgct gtggggcatc cccctcgtca tcgatttcga ggggaaagag
     2821 gagcgctatt cgtgactgaa gccctcctga tcgtaggggc ctacctcgtg ggatcgatct
     2881 ccccgagcgt gttcctcggt cggctggtca gagggatcga tctcagggaa cacggcagcg
     2941 gcaatgccgg cacgaccaac gcctttcgcg ttctcggcac cgggcttggc ctcgcggtct
     3001 tggtggctga cgtggccaag gggttcgttg cggcactgac agcgagcctt cttgtcggcc
     3061 ctacagctac tgtggtcgtg gctttggcgg tgatggccgg acacaactgg tccatattcc
     3121 tacgcggcaa gggcggcaag gggg
//
LOCUS       DASRMJ010000038         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_77890, whole genome shotgun sequence.
ACCESSION   DASRMJ010000038 DASRMJ010000000
VERSION     DASRMJ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77890"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..772)
                     /locus_tag="VFD74_01125"
     CDS             complement(92..772)
                     /locus_tag="VFD74_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZK48188.1"
                     /translation="MNAARDDGNATVYTAEQIWAIGRLIAGIVHEINNPLTSAGGLAD
                     LLRSEVDDETARGDLETITEEITRAVNIVRNLRAFAIHPDDEAKLCSLDRLAAQVVET
                     RGYESRGRGLHLESDLGAADRAIWGAPPKMLLLILMLVLRAETRLLAEEGEPTVPRRI
                     VVRTSASPEHVTLAVEDNSLVAAADADPTLAACAAAAAELDGDLTLDGAPGGGSLVTV
                     TFPAAREA"
     gene            complement(804..1787)
                     /gene="trpS"
                     /locus_tag="VFD74_01130"
     CDS             complement(804..1787)
                     /gene="trpS"
                     /locus_tag="VFD74_01130"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZK48189.1"
                     /translation="MRILSGIQPSGRLHIGNYFGAIRQHLQLQAENEAFYFMASYHAL
                     TTVQNPAELRALTLDVATDYLALGLDPARTALFTQQDVPEVTELAWILSCVTPMGLLE
                     RAVSYKDKVAQGLMANHGLFAYPVLQAADILIYGSHLVPVGQDQRQHIEITRDIAAKF
                     NAQFGDVLVIPDAYILESTAVVPGVDGRKMSKSYGNDIGMFEEGRALKKKVMSIVTDS
                     AAVEDPKDPEGSTPFMLLRLMAHPQEVEEWAARYRAGGMGYGEVKKRVLELLDDTFGP
                     FRERRHALEADPDHVHEVLRAGGRRARRVAHDMMERVREAVGLPISYDPPD"
     gene            2170..3402
                     /locus_tag="VFD74_01135"
     CDS             2170..3402
                     /locus_tag="VFD74_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZK48190.1"
                     /translation="MNIQEEALQLHAEWRGKIEVTSRVAVGCKHDLSTAYTPGVAEPC
                     RAIHKDPSLVDLYTRRWNLVAVVSDGSAVLGLGNIGPKAALPVMEGKAVLFKAFAGVD
                     AFPICLDSQDPDEIVRAVQLVVPTFGGVNLEDISAPRCFEIEKRLKETTDIPIFHDDQ
                     HGTAVVVMSGIINSCKITGRALSDLHVVVNGAGAAGIACAKLLLDVGVKDLVLCDSHG
                     IVCSERDDLNPVKREMLQRTNRDDKCGSLADALEGANCFIGVSAKDAVKPAMVRSMQR
                     DPIIFAMANPDPEIHPELARQAGAAVVGTGRSDFPNQVNNVLGFPGIFRGALDVKATD
                     ITERMKIAAAHALADLVGDDLSPEHVMPEPFDLRVAPAVAMAVATAAIEEGIARDPKD
                     PELVKRMAEQMVGLTRGS"
     gene            3483..4328
                     /locus_tag="VFD74_01140"
     CDS             3483..4328
                     /locus_tag="VFD74_01140"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZK48191.1"
                     /translation="MREIDVSEVTSAVARLSMDACYYLGGDVVAFFEGAVEREESEAG
                     RDVFRQLLENAEIARREQMPLCQDTGLAVVFMDVGQDVHFVGGSVVEAVDEGVRQGYS
                     EGYLRKSSVEAPFSERKNTGDNTPAVLHTRIVPGDRVKIILAPKGGGAENMSALTMLK
                     PAQGRQGVVDFVVDTVSKAGPNACPPMVVGVGVGGTFERAALLAKRALMRNVGSGNPE
                     PRLAELEDELEARCNDLGIGPQGLGGRITVMDVFVEEAPAHIASLPVAVNIQCHSARH
                     KEVVL"
     gene            4328..>4442
                     /locus_tag="VFD74_01145"
     CDS             4328..>4442
                     /locus_tag="VFD74_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006692615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HZK48192.1"
                     /translation="MSETIAITTPLTEEAVAGLRAGDRVLITGTLLAARDAA"
BASE COUNT          727 a         1416 c         1548 g          751 t
ORIGIN      
        1 gcgctgaacc gcgcggggcc ccggctcgac gcacctgatc cgccggccgg gggcttgcct
       61 cccggccggc ccacgtcagg gccggctcag ttcaggcctc gcgcgcagcc gggaaggtca
      121 cagtcaccag gctgccgccg ccgggtgcgc cgtccagcgt aaggtcgccg tctaactctg
      181 cggcggcggc tgcacaggcg gccagcgtgg ggtcggcgtc cgctgccgcc acgagcgagt
      241 tgtcctcgac ggccagtgtg acgtgttcgg gagaggccga ggtgcggacc acgatgcggc
      301 gaggcacggt gggctcgcct tcttcggcca ggagcctcgt ctcggcccgc agcaccaaca
      361 tgaggatcaa gagcagcatc ttcggcgggg cgccccagat cgcgcggtcg gccgcaccga
      421 gatccgattc gagatggagg ccgcgccccc gagactcgta gccacgagtc tcgaccacct
      481 gagcggccag tcggtcgagc gagcagagct tcgcctcgtc atcggggtgg atcgcgaacg
      541 cgcgaagatt gcgcacgatg ttgaccgcgc gggtgatctc ctcggtgatc gtctcgagat
      601 cgccgcgggc ggtctcgtca tcgacttcgg agcggagcag gtcggccaag cccccagccg
      661 aggtgagggg gttgttgatc tcgtgcacga ttcccgcgat gagccgaccg atcgcccaga
      721 tctgctccgc ggtgtagacc gtcgcgttcc cgtcatcgcg agccgcgttc atgagatccc
      781 tctcccaccg cgttggacgg cggtcagtcc ggcgggtcgt aggatatggg caggcccacc
      841 gcttcccgca cccgctccat catatcgtgc gccacgcggc gcgcgcgccg gccgccggcg
      901 cggagcacct cgtgcacgtg atccggatcg gcctcgaggg cgtggcgtcg ctcccggaag
      961 gggccgaagg tgtcatccaa gagttcgagc acgcgcttct tgacttcccc atagcccatg
     1021 ccgccggctc gatagcgggc ggcccactcc tcgacctcct ggggatgcgc catgagtcgc
     1081 agaagcatga aaggagtgga tccctccgga tccttgggat cctccaccgc ggccgagtcg
     1141 gtcacgatgc tcatgacctt cttcttgagc gcccttcctt cttcgaacat cccgatgtcg
     1201 ttgccgtagc tcttggacat cttgcgcccg tccacaccgg gaacgaccgc cgtcgactcc
     1261 aggatgtagg cgtcggggat cacgaggacg tcgccgaact gcgcgttgaa tttcgcggct
     1321 atgtcgcgtg tgatctctat gtgttgccgc tggtcttgcc ccacggggac caggtgcgaa
     1381 ccgtagatca agatgtccgc agcctggagc accggatagg cgaacagccc atggttggcc
     1441 atgaggccct gggcgacctt gtccttgtag ctcaccgcgc gctcgagcag acccatcgga
     1501 gtcacgcacg agaggatcca cgcgagttcg gtgacctcgg gcacgtcctg ctgggtgaag
     1561 agggcggttc gcgcggggtc gagcccgagc gcgaggtagt ccgtggccac gtcgagggtg
     1621 agtgcgcgca gttcggccgg gttctgaacg gtggtgagcg cgtggtagct ggccatgaaa
     1681 tagaaggcct cgttctcggc ctggagctgc aggtgctggc ggatggcgcc aaagtagttg
     1741 cctatgtgca ggcgccccga tggctgtatt cccgacagta tccgcatggt cgtaccctcg
     1801 ccttccttac gtctctgtgt ttgccggcgc ctccggcctc gagcacccgg ccgtgtagct
     1861 tcgcgagtct accccggcgc cggacgccgc ggcaagcggg cccgcttgct ggtatcgtga
     1921 gggcaagtat gttagaaata ccacaatcgc ccacgggcgt acacatagct gtcgctgcgg
     1981 cgaccgtggc cggtccggag ggcgttctcc ccgggttgcc ggcgcggtgg gagctctggt
     2041 gatccagcgt cgatgccttt tctagatcat gaacgatcga aggcgccggt tgctggattt
     2101 tttgcattcc aggggccgac gtggaatcgg gagcaaccga gccccggggc cggagcaagg
     2161 aggatcggct tgaacatcca ggaagaggcg ctacagcttc acgcggagtg gcggggcaag
     2221 atcgaggtga cgagccgggt ggctgtcggc tgcaagcacg atctctccac cgcatacaca
     2281 ccaggggtcg cggagccgtg tcgcgcgatc cacaaggacc cttctctcgt agacctgtac
     2341 acgaggcgct ggaatctcgt ggccgttgtg tccgacgggt cggccgtgct gggactcggc
     2401 aacatcgggc cgaaggcggc cctgccggtg atggagggca aagccgtgct cttcaaggcg
     2461 ttcgcggggg tggacgcgtt ccctatctgt ctcgattcgc aggatcccga cgagatcgtg
     2521 cgggcggtgc agttggtcgt tcccaccttc gggggagtga acctcgagga catctcggcg
     2581 ccacgctgtt tcgagattga gaagcgcctc aaagagacca cagacatccc catcttccac
     2641 gacgaccagc acggaacagc cgtcgtcgtg atgtcgggga tcatcaactc gtgcaagatc
     2701 accgggcgtg cgttgagcga cctgcacgtg gtcgtgaacg gagccggtgc ggctgggatc
     2761 gcgtgcgcca agctcctgct cgacgtgggc gtcaaggacc tcgtgttgtg cgacagtcac
     2821 ggcatcgtgt gctccgaacg tgacgacctc aacccggtca agcgtgagat gttgcagagg
     2881 accaaccgcg acgacaagtg cgggtcgctg gccgacgcgc tggaaggcgc gaattgcttc
     2941 atcggggtgt ccgcgaagga tgcggtgaaa cccgccatgg tgcgatcgat gcagagagac
     3001 ccgatcatct tcgccatggc caatcccgac cccgagatcc acccggagct ggcccgccag
     3061 gccggcgcgg ccgtcgtggg aacgggtcgc agtgacttcc ccaatcaggt gaacaacgtg
     3121 ctcgggttcc cggggatctt ccgcggcgct ctcgacgtca aggccacgga tatcaccgag
     3181 cggatgaaga tcgcggccgc tcacgccctg gctgatctgg tgggcgacga cctctctccc
     3241 gagcacgtga tgcccgagcc tttcgatctg cgggtcgccc cggctgtggc catggctgtg
     3301 gccacagccg ccatcgagga agggatcgcc cgcgacccga aggacccgga gctggtgaag
     3361 aggatggccg agcagatggt cggtctcaca cgtgggagct gacctgtgaa gtccgacgcg
     3421 gcatgtggat cggggtcggc gcgccgcgag cggccgctcc gcgcgctggg gaaggagtga
     3481 acgtgcgaga gatcgatgtg tccgaggtca cctccgccgt ggcgcggctg tccatggacg
     3541 cctgctacta cttgggcggc gacgtggtgg ccttcttcga aggtgccgtg gaacgcgagg
     3601 agtcggaagc gggccgcgac gtgttccgcc aactcctcga gaacgcggag atcgcccggc
     3661 gcgaacagat gccgctctgc caggacaccg gcctggcggt ggtgttcatg gacgtgggcc
     3721 aggatgtgca cttcgtcgga ggcagcgtcg tggaggccgt ggatgaggga gtgcggcagg
     3781 gctactccga gggatacctg aggaagtcct cggtcgaggc gcccttctcc gagcgcaaga
     3841 acaccggcga caataccccg gccgtgctcc acacccgcat cgttccgggc gacagggtca
     3901 agatcatctt ggcgcccaaa ggcggagggg ctgagaacat gagtgctctc actatgctca
     3961 agcccgcgca gggtcgccaa ggcgtggtcg atttcgtggt ggacacggtg tcgaaggccg
     4021 gcccgaacgc ctgcccgccg atggtggtgg gcgtgggcgt gggcgggacg ttcgagaggg
     4081 cggccctcct ggccaagcgg gctctgatga gaaacgtcgg ctccgggaac cccgagcctc
     4141 ggctggccga actcgaagac gaactggagg cgcgctgcaa cgacctcggc atcggcccgc
     4201 agggcctcgg aggccggatc accgtgatgg acgtgttcgt ggaggaagcg ccggcccaca
     4261 tcgcgtcgct gccggtggcc gtgaatatcc agtgccactc cgcccgccac aaggaggtgg
     4321 tgctctgatg agcgagacca tcgccatcac caccccactc accgaggagg ccgtcgccgg
     4381 gctgcgcgcc ggcgaccggg tgctcatcac cggcacgctc ttggccgccc gcgacgctgc
     4441 gc
//
LOCUS       DASRMJ010000039         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_402544, whole genome shotgun sequence.
ACCESSION   DASRMJ010000039 DASRMJ010000000
VERSION     DASRMJ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402544"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFD74_01150"
     CDS             complement(<1..227)
                     /locus_tag="VFD74_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HZK48193.1"
                     /translation="MRNEAAQDLSQFQIPDGFRGRSRVAVQLWWLVQSTVFRWSPQVA
                     YGFRRWLLRRFGAKVGSHVLVRPSASTTYPW"
     gene            complement(217..633)
                     /locus_tag="VFD74_01155"
                     /pseudo
     CDS             complement(217..633)
                     /locus_tag="VFD74_01155"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            812..1959
                     /locus_tag="VFD74_01160"
     CDS             join(812..1073,1073..1959)
                     /locus_tag="VFD74_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611761.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZK48194.1"
                     /translation="MPKGKKHTPEQIVRHLRDAEAALAAGRTVGQICQEIGVSEQTYY
                     RWKTLYGGMKTQELRRLKELEQENLRLKKLVADLSLDNAMLKEFGRGKLLSPARKRRA
                     VNHLQATFPCSERRAARVVSVSRSTLRYEARLPDDEPRLLSRMEVLVRKHPRYGYRRI
                     GDLLRREGFRANPKRIYRLWKREGYRVARAQHKKRRLGAGENACHRHRPERRNHIWAL
                     DFIFDRDEGGRSLKWLTVVDEYTRECPKLLCERSITAQDVIDELIRVMQIRGVPGHIR
                     ADNGPEFIAAAIRTWLRKAGIGTLFVEPGAPWENGYAESFHARLRDEFLNAEAFANLR
                     EAQALGEQWRREYNHERPHSSLGSLTPEEFAAGLTALPVGATGTCQPQ"
BASE COUNT          414 a          714 c          623 g          327 t
ORIGIN      
        1 ttccaagggt aggtggtaga cgcggacggg cgaaccagca catgactccc cacctttgcg
       61 ccgaagcgac ggagcagcca gcgtctgaaa ccataggcta cttgcgggga ccaccggaat
      121 accgtcgact ggaccagcca ccatagttgc acggccaccc gcgagcgacc gcggaaaccg
      181 tcaggtattt ggaactgcga caggtcttgc gcggcctcat ttctcacgag gttgcccacc
      241 tacgtgctcc ccaataatct ccaccaaccg ttcggcggcc tgttgcacat gaaagcgcga
      301 ggcaaagcat tgctgcgccc gcgcactcat agccccatag cctgcgcgat ccagctccaa
      361 ccaacgtctc aagttcctca ccgtgccgta taccgtgtcc gtgtccacaa acccagcggc
      421 gtcggactca atctcccgcc agatgttcac tttgtcactg atcagcaccg gctttccgta
      481 cgcgagggct tcagcgacaa caataccaaa gttttcctgg tgggagggca ggcagaacac
      541 ctcagccgcc ctgaaggccg cccacttcat gtcgccttgg agcatgcccg cccacgtgat
      601 gcgttgcgca atccccagcc gctcggcctg cgcctggagc gtagtctgtc agccggtctg
      661 gtccggcccg gccatcacca ggtgcagccg ttcatcgccg ttcgcgactt gggcgaaggc
      721 gtccaagagg aggtggaatg ccccccgcca gccgtaccac gtagaatggg agtcctgacc
      781 cggcccacgg ggccgagaaa ggaccaccgc catgccgaag gggaagaagc acacaccgga
      841 gcagatcgtc cgacacctgc gcgatgccga agccgctctg gccgccggcc gcaccgtcgg
      901 ccagatctgc caggagatcg gagtcagcga acagacctac taccgctgga agaccctcta
      961 cgggggcatg aagacgcagg aactccggcg cctgaaagag ctcgaacagg agaacctgcg
     1021 cttaaagaag ctggtagccg atctctccct ggacaacgcc atgctgaagg agttggccga
     1081 gggaaacttc taagcccggc ccggaagaga agagccgtga atcatctcca ggctaccttc
     1141 ccttgttccg aacgccgggc ggcccgtgtc gtctccgtga gccgctccac gctccgctat
     1201 gaggcccgcc tgcccgatga cgagcctcgg ctcctctccc gcatggaggt gctcgtccgg
     1261 aagcaccccc gctacggcta ccgcaggata ggggacctcc tacgccggga gggcttccga
     1321 gccaacccga agcgcatcta ccgcctctgg aagcgcgaag gctaccgagt ggcgcgcgct
     1381 cagcacaaga aacggcgcct gggtgcaggc gagaacgcct gtcaccgcca ccgccccgaa
     1441 cgcaggaacc acatctgggc cctcgacttc atcttcgacc gcgacgaagg cggccgctcc
     1501 ttgaaatggc tcaccgtcgt cgacgagtac actcgggagt gcccgaagct cctctgcgaa
     1561 cgcagcatca ccgcgcagga cgtgatcgac gaactcatcc gcgtcatgca gatccggggt
     1621 gtcccgggcc acatccgcgc ggacaacgga cccgagttca tcgcagcggc catccgcacc
     1681 tggcttcgca aagcggggat tggtaccctc ttcgtggagc cgggcgcccc ctgggagaac
     1741 ggctacgccg aatccttcca cgcgcgtctg cgggatgagt tcctaaacgc cgaagccttc
     1801 gccaacctcc gggaagccca ggctctggga gagcagtggc gccgggagta caatcacgag
     1861 cggccgcata gctctctcgg gtcactgacc ccggaagagt tcgccgccgg gctgactgcc
     1921 cttccggtcg gggctaccgg aacctgtcaa ccccaatgag acactcagcg ttcgcaaatt
     1981 agtgagtagc ctcctttgcc gcgggtttgt tgatccaggc tgcggtgggc agcgccgggg
     2041 gggtggcggc cgcaagacaa agcgctccgg ggtgcggc
//
LOCUS       DASRMJ010000040         3245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_114026, whole genome shotgun sequence.
ACCESSION   DASRMJ010000040 DASRMJ010000000
VERSION     DASRMJ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114026"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VFD74_01165"
     CDS             complement(<1..1095)
                     /locus_tag="VFD74_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK48195.1"
                     /translation="MYCQDCGVEVPQDSVFCQHCGARLPEAPDTGAGRRPGTRTFLGD
                     PADPSAYVTPTSGSIPGMPPYAGPPPTGESSGSRSGNSTPQPRRAGGNRTLLIAVVVA
                     VALFMLAVPLGLMALLTTTRESSGTSDPVSVSSGEDTVTTAPSDGDSTSSTSGSGPPG
                     SGSTQGVAPDYASSDWEELELPGLPEQTYSAITSDVAAAFETKSGLYAYIYETGKLVH
                     LPTEMNAASSVSIDGDRMVWWEGDYDESDTLTREGIFSYRLPDGPRVELLTGGESAGS
                     PLLSDGYVTWLEGRESADHPQDIWELPIYGAALDAGGRLQGEPQSLVENPRSYVLGDS
                     TWSYDLTPDYLAWEDNDPADGSQPGTWVLDR"
     gene            complement(1229..1645)
                     /locus_tag="VFD74_01170"
     CDS             complement(1229..1645)
                     /locus_tag="VFD74_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZK48196.1"
                     /translation="MGVRSLVIDTNAYAAFMAGDEDTVRTLQTADRIVVGVVVLGELL
                     AGFAAGSRRAENRRDLSAFLASPRVQVAPASPTTAEFYAAVFAQLRRKGRPLPANDLW
                     IAATAFEHGAPLLTRDAHFREIEGLLLAQAPEDLVP"
     gene            complement(1636..2013)
                     /locus_tag="VFD74_01175"
     CDS             complement(1636..2013)
                     /locus_tag="VFD74_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48197.1"
                     /translation="MRQRASERYGRATAAYALTWTLTALGAIMTPHILQSRDRGLEMT
                     VLTVRGLGEELAAALRREAKGRGISMNTLALQLLSKGLGLNSEPTLHRDLDDLAGRWT
                     GDQLAEFMAASADFERIDAGLWE"
     gene            complement(2063..2581)
                     /locus_tag="VFD74_01180"
     CDS             complement(2063..2581)
                     /locus_tag="VFD74_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HZK48198.1"
                     /translation="MELTPFDHLEESDLRRYLEFLLWHYRVVDAFWFLRVADRFDQPT
                     AESINEEVWERVSGMAAKDLVARFDIREGGLSGFVRALRLFPWTILVGYQITESEDEV
                     TVEVPECPTQEARRRRGLDEYICREMHRLEFEGFARVIDPRIRVSCDFAPPGDRPEGL
                     DCRWRFTLAPPF"
     gene            complement(2678..2785)
                     /locus_tag="VFD74_01185"
     CDS             complement(2678..2785)
                     /locus_tag="VFD74_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48199.1"
                     /translation="MVRLDRFYLENWTIGLDLKILARTVFVVLGHKGAY"
     gene            complement(2779..>3245)
                     /locus_tag="VFD74_01190"
     CDS             complement(2779..>3245)
                     /locus_tag="VFD74_01190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZK48200.1"
                     /translation="LGTGVLVLGSPLWLAVALAVKVTSRGPVLFHQERVGCKGRHFDM
                     VKFRTMTADAEARLAELADENEAEGPLFKMKDDPRVTRVGGFLRRWSIDEIPQMLNVM
                     GGEMSIVGPRPPLPGEVERYETEHLCRLKGIPGITGLFGRCPAAATSPSTTW"
BASE COUNT          516 a         1049 c         1165 g          515 t
ORIGIN      
        1 gcggtcgagc acccaggttc cgggctggct gccgtcggcg gggtcgttgt cttcccacgc
       61 caggtagtcg ggcgtcaggt cgtacgacca ggtggagtca cccagcacgt aggagcgggg
      121 gttctccacc agcgactgcg gttctccctg cagcctgccg ccggcatcga gcgcggctcc
      181 gtagatgggt agctcccaga tgtcctgcgg gtgatccgcc gattcgcggc cctccagcca
      241 ggtgacgtag ccatcggaga ggagcggaga gcctgcggac tccccgcccg tgagcaactc
      301 cacgcggggg ccgtccggca ggcggtagct gaagatgcct tcgcgggtca gggtgtcgct
      361 ctcgtcgtag tcgccctccc accacaccat tcggtcgccg tcgatggaga ccgaggaggc
      421 ggcgttcatc tcggtgggga ggtgcacgag tttgccggtt tcgtagatgt acgcgtagag
      481 gccggatttg gtctcgaagg ccgctgcaac gtccgaggtg atagcggagt aggtctgctc
      541 cgggagtccg ggcagttcca gttcctccca gtcgctcgag gcgtagtccg gggcgactcc
      601 ctgcgtcgaa ccggagccgg gcgggccgga gcccgaggtg gaggaggtgg agtcgccgtc
      661 ggagggggcc gtggtgaccg tgtcctcacc cgaggagacc gagacgggat cggacgtgcc
      721 cgacgactcc ctggtggtgg tcaggagtgc catcaacccc aaaggaacgg ccagcatgaa
      781 tagagcgacc gcgacgacca cggcgatgag cagggtgcgg ttgcccccgg cgcgtcgcgg
      841 ctggggggtg gagttgccgc tccgactgcc ggacgattcg ccggtcggcg gcggcccggc
      901 gtaggggggc atgccgggaa tcgaccccga ggtcggcgtc acgtacgcgg acggatcagc
      961 gggatcccct aggaaggtcc tcgtgccggg gcgtcggccg gcgcctgtgt cgggtgcctc
     1021 gggcagtcgt gcgccgcagt gttggcagaa caccgagtcc tgcggcacct ctacaccaca
     1081 gtcctgacag tacatgcggc acctccttcg tcggtcgctg cgcggaccag ggggcgggcc
     1141 cgtccttggc ggtccggccg tcgagcggtg acgttcgcgg caaaggtacc aggtgcgggg
     1201 ccgggcgtga agcgcgcgcc gttcgaccct acggcaccag gtcctcaggc gcctgcgcga
     1261 gcaggaggcc ttcgatctcc ctgaagtgcg cgtctcgggt caggagggga gcgccgtgtt
     1321 cgaacgccgt cgcagcgatc cacaggtcgt tcgcggggag ggggcggccc ttccggcgaa
     1381 gctgagcgaa cacggcggcg tagaactccg cggtggtcgg ggatgcgggc gcaacctgca
     1441 cacgcgggga ggcgaggaac gcggaaagat ctcggcggtt ctcggccctc cgcgagccgg
     1501 cggcgaagcc cgcgagcagt tcgcccaaca cgaccacgcc gaccacgatg cgatccgctg
     1561 tttgaagcgt ccgcacggta tcttcgtcac cggccatgaa ggcggcgtag gcgttggtgt
     1621 cgatgacgag ggacctcact cccacagccc tgcgtcaatc cgctcgaagt cggcgctagc
     1681 ggccatgaac tcggccaatt ggtcgccggt ccacctgccg gccaggtcgt cgaggtcgcg
     1741 gtggagcgtg ggttcggagt tcaggccgag ccccttgctc aacaactgca acgccagggt
     1801 gttcatgctg atccctcggc ccttggcttc cctgcgcagt gctgccgcaa gttcctcgcc
     1861 aagacctcga acggtcagga cggtcatctc caagcccctg tctcgtgact gcagaatatg
     1921 cggtgtcatg atagccccaa gtgcagtcag agtccaggtg agggcgtagg ccgccgtcgc
     1981 acgaccgtag cgctcggaag ctctctgccg catcagactt cgggtggatt caggtgcgcg
     2041 tgggggcggg atgcgccgaa tgtcagaagg gcggggccaa cgtgaagcgc cagcggcagt
     2101 cgaggccctc ggggcggtcg ccgggcgggg cgaagtcgca gctcacgcgg atgcgtgggt
     2161 cgatcacccg ggcgaagcct tcgaactcga gtcggtgcat ctcgcggcag atgtactcgt
     2221 cgaggccgcg ccgtcgccgg gcctcctgcg tggggcactc gggcacctcg acggtcactt
     2281 cgtcctcgga ctcggtgatc tggtagccga cgaggatggt ccaggggaag aggcgcagcg
     2341 cccgcacgaa gcccgagagg ccgccctcgc ggatgtcgaa gcgcgcgaca agatccttgg
     2401 cggccatgcc tgacacgcgc tcccatacct cctcgttgat ggactcggcc gtgggctggt
     2461 cgaagcggtc ggccacgcgc aggaaccaaa aggcgtcgac cactcggtag tgccacagca
     2521 ggaactccag gtaacggcgg aggtcggact cctccagatg gtcgaagggg gtcagttcca
     2581 cgggcgggtc aggcggtcga ggggtggtgg tccagggaca ttctcgggcg gctcgtcatg
     2641 cgttcagcct accatgtcgc tacgcccggg cgagcgctca atacgcgccc ttgtgcccga
     2701 gcaccacgaa gacagtgcgc gctaggatct tcaggtcgag gccgatggtc cagttctcta
     2761 ggtagaagcg gtcgagcctc accatgtcgt cgaaggagag gtcgctgcgg ccggacacct
     2821 gccaaaaagc ccggtgatgc cggggatgcc cttcagacga cagaggtgct ccgtctcgta
     2881 ccgctccacc tcgccgggga ggggcggccg gggccccacg atgctcatct ccccccccat
     2941 gacgttcagc atctgcggga tctcgtcgat gctccaccgg cgcaggaacc cgcccacgcg
     3001 ggtgacgcgc gggtcgtcct tcatcttgaa gagggggcct tcggcctcgt tctcgtcggc
     3061 caactcggcc aaacgcgcct cggcgtccgc ggtcatcgtg cggaacttga ccatgtcgaa
     3121 gtgacggccc ttgcagccca cccgctcctg atggaagagg acagggcccc gcgaggtcac
     3181 cttcaccgcc agcgccaccg ccagccacag gggcgatccg agcacgagca cgcctgtgcc
     3241 gaggg
//
LOCUS       DASRMJ010000041         5798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_438800, whole genome shotgun sequence.
ACCESSION   DASRMJ010000041 DASRMJ010000000
VERSION     DASRMJ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438800"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFD74_01195"
     CDS             complement(<1..330)
                     /locus_tag="VFD74_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZK48201.1"
                     /translation="MSSSKLYVGNFTYDTTESTIEALFAPHGSVTSVALITDRDSGRP
                     KGFGFVEMGTSQEAQAAKFALDGTDLGGRALKVDAAKEREPRTGGNRGGGYGGGGGSY
                     GGGGGNRW"
     gene            600..1037
                     /locus_tag="VFD74_01200"
     CDS             600..1037
                     /locus_tag="VFD74_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HZK48202.1"
                     /translation="MFRQHTRFVTIVFVSALLVALAPTTFAGCSSGAGDLDGTHWKLV
                     EWTVNSLFPGDFAIRAQFADGQISGTSGVNSYSGPYKADGDSFEVSAVAGTLMAGPEP
                     AMRAEAAYLTLLQQARLFAVSGSTLTLYDNGGNVSLVFEAVAD"
     gene            1186..1953
                     /locus_tag="VFD74_01205"
     CDS             1186..1953
                     /locus_tag="VFD74_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48203.1"
                     /translation="MTVTDSFWSHWAWPLATAVLGLVFAGLVFRQYLKRRKAHQAAWS
                     VGLLFYGLAAVFEAWSEMSDHWDPTVYRFYIVMAASLVGFLGLGSLYLIARRQWLGHL
                     YLGFNLVALAVFLYGVFTVQLDLTKLVPGITVGGQALGASLSFPRAMSLVFNIPGSIF
                     LLGGALLSIYRFSRKKEYAYRMWANVLIAAGTILIAYAGSRARLGVTPGLYPAEMFAS
                     ILLLAGFLLAGSLRTGAKAIGERLAADRGGIDHGPAE"
     gene            complement(2003..2632)
                     /locus_tag="VFD74_01210"
     CDS             complement(2003..2632)
                     /locus_tag="VFD74_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HZK48204.1"
                     /translation="MRTGGHVGALVPGLLLAWDAATGGLSVDPVKELTQRTGKDAFVL
                     LVLSLAVGPIVRITGLRTVAPLARILGLYAFGYATLHLLAFVGLDFGFRGDLIVDGVL
                     EKRFALIGLTAFLILLVLTATSTRARVRRLGPIWKRIHRLAYLAGAFATLHYLWAVKT
                     ITREQLAWAGVVGVLLALRLPEAAGLFVLTRRKLTRERPPRDGPPTAES"
     gene            complement(2710..3663)
                     /gene="msrP"
                     /locus_tag="VFD74_01215"
     CDS             complement(2710..3663)
                     /gene="msrP"
                     /locus_tag="VFD74_01215"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HZK48205.1"
                     /translation="MNRIDPSEITPEHIYLSRRSFMVGVAATAGAAVLAGCEVGGDTG
                     TSGEGASTTVGTPGSTGAATDELGQGLTSYEAVTTYNNFYEFTVDKEEVAEKAADFVS
                     TPWTLEVGGLVDSPRTFTIADLLALLPEEERIYRMRCVEGWSMVIPWLGIPLGALLEM
                     VGPTSEARFVRFQSIDDPEHLPGQKKYSYDWPYIEGLRLDEATHDLTLLSTGLYGKRL
                     LPQNGAPIRLVVPWKYGFKSIKSIVKIELVREMPVSFWMASSPREYGFYANVNPTVDH
                     PRWSQKAERRIGEFGRRDTLMFNGYDEVASLYEGMDLRKDF"
     gene            3818..>5798
                     /locus_tag="VFD74_01220"
     CDS             3818..>5798
                     /locus_tag="VFD74_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZK48206.1"
                     /translation="MANRLIGETSPYLLQHAHNPVDWYPWGEEALAAARSQRRPVFLS
                     IGYAACHWCHVMERESFEDVETAALMNERFINIKVDREERPDLDSIYMRAVTVLTGRG
                     GWPMSVWITPEGAPFFGGTYFPDTPRHDIPSFAQVLEALADAWESRPDEIAASAAKLA
                     GKLREDAGLPVLRRTMDKGDDDARVAAGAASADLVPAPAGLDPETGAEASLALISGFD
                     AANGGWGEAPKFPQPMALEFLLRRWVRHGDEHARILVERSLDFMAVGGIYDQLGGGFH
                     RYSVDAEWLVPHFEKMLYDNSQLARVYTHAWQALGHERYRQVAEETLDYVLREMTHPD
                     GGFFSTQDADSEGVEGKFFVWTPDEIEALLDADAADAGGDAADQCLGATDRHSGEAGI
                     DAARLRRHADAALFMEAYGVEPGGNFEGASILSRVRTDEDLARAEGDSAATVAARLAA
                     ARARLLTAREERAHPARDEKILAAWNGLMIAAFADAAAAFGRSDYLLAAERAAAFVLQ
                     RLRGPDGRLMRSWRDGSTRHNGYLEDHAHVIEGLLALYQADFDPRWFVAARDLADEML
                     ASFGSAESGFYDTRDDHESLFLRPQDYQDNAVPSGNAMAATVLLKLRAYTGEARYAAP
                     AERTLLGLEPLLSRHPLAFGQGLIAFDLAVGEGGEV"
BASE COUNT          934 a         1900 c         1964 g         1000 t
ORIGIN      
        1 ccagcggttt ccgccgccgc cgccataact gccgccgccg ccaccgtaac cgccgccgcg
       61 gttgccaccg gtgcgaggct cacgctcttt ggctgcgtcg accttgaggg cgcggccgcc
      121 cagatcggtt ccgtcgaggg cgaacttagc cgcctgcgct tcttgagacg tgcccatttc
      181 cacgaaaccg aagcccttgg ggcgtccgct atcgcgatcg gtaatgaggg ctaccgaagt
      241 caccgaaccg tgcggcgcga agagcgcctc gatcgtggac tcggttgtgt cgtaggtgaa
      301 gttgcctacg tacagcttgg acgaactcat gaaatcctcc gttgcagaga gtacattact
      361 gcatgcgaag aggcatacac tgcaaacggg gcggagagaa cacgcgaaga gttcggcgcg
      421 gcgcctgaag agagacccta tcgcatacga taataagtat agcacacccg aatgatggcg
      481 gagcgaacgg caaacagaac ccggctcgat cacccacggg cgccgagtgg cgatgcagcg
      541 agtcactgat ggtggggagc cactgatggc agggagccgc caataggagg gtgtcactga
      601 tgtttcgtca gcacacaagg ttcgtgacga tcgtcttcgt gtcggctctc ctggtcgcgc
      661 ttgcgccgac gacgttcgcg ggctgctcga gcggggccgg ggatctggac ggaacgcact
      721 ggaagctggt cgagtggacc gtgaactcac tcttcccggg ggacttcgcc atcagggctc
      781 agttcgccga tgggcagatc tcgggcacaa gcggcgtcaa cagctactcc ggaccgtaca
      841 aggccgacgg cgactccttc gaggtgagcg cggtggccgg cacactcatg gccgggccgg
      901 agcccgcgat gcgcgcggag gccgcctacc tgaccctgct gcagcaggcg cggttgttcg
      961 ctgtgagcgg cagcactctc acgctctacg acaacggcgg caacgtgtcg ttggtcttcg
     1021 aggccgtggc cgactaggtt gcggtcgcag gccgcagggt cgcggaggcg ctgcgaaagc
     1081 gcaacccgcc cggcgggtgc ggggatgtac aatgtgtcgt ccagcattcg ccaaccccgc
     1141 agggcggact cccgcgggag aggtcccttg gggggatttc ggaggttgac cgtgactgac
     1201 tccttctggt cgcactgggc ctggccgctc gcgactgcgg ttctcggcct cgtcttcgcg
     1261 ggactggtgt tccgccaata cctcaagcgc cgcaaagcgc accaggccgc ctggtcggtg
     1321 ggcctgctct tctacgggtt ggccgccgtc ttcgaagcct ggtcggagat gtcggaccac
     1381 tgggatccga cggtctaccg tttctacatc gtgatggcgg cgtcgctggt gggcttcctc
     1441 ggtctggggt cgctgtatct gatcgcccgc cgtcagtggt tggggcacct ctatctcggc
     1501 ttcaacctgg tggctctggc cgtcttcctc tacggggtct tcaccgtcca gctggacctg
     1561 accaagctgg tgcccgggat cacggtcggt gggcaggcct tgggcgcgag cctctccttc
     1621 ccgcgtgcca tgtcgctggt cttcaacatc cccggctcga tcttcctact agggggagcg
     1681 ttgctttcca tctatcggtt ttcgcgtaag aaggagtatg cgtatcgcat gtgggccaac
     1741 gtcctcatcg cggcaggcac catcctcatc gcctacgcgg gttcacgtgc gcggctcggc
     1801 gtgactcccg ggctctatcc ggccgagatg ttcgccagca tcctcctgct cgccggcttc
     1861 ctcctggcgg gatcgcttcg cacgggcgcc aaggccatcg gggagaggct tgccgccgac
     1921 cgcggaggca tcgaccacgg acccgcggag taggggttcg gccgggagcg cgcgggtccg
     1981 ccgcggttct tccgccgacg gcctacgact cggccgtggg agggccgtca cgtggcggcc
     2041 gttccctcgt gagcttgcgg cgcgtaagga cgaacagtcc ggctgcctcc ggtagtctca
     2101 gtgccagcag caccccaacc actcccgccc aggccaactg ctcccgggtg atggtcttga
     2161 cagcccacag gtagtggagc gtggcgaacg ctcccgccag gtacgccagt cggtggatgc
     2221 gcttccagat gggcccgaga cgtcggaccc gcgcgcgggt ggatgtggcc gtcaggacca
     2281 ggaggatcag gaacgccgtc agcccgatca gagcgaagcg cttctcgagc accccgtcca
     2341 cgatgaggtc gccgcggaac ccgaaatcga gacccacgaa agccaagagg tgcagcgttg
     2401 cgtagccgaa cgcgtagagt ccgaggatgc gggccagcgg cgcaaccgtt cgaaggccgg
     2461 tgatccgtac tatcgggccc accgcaagtg agagcaccag gaggacgaag gcgtccttgc
     2521 cggtgcgctg ggtgagttcc ttcaccgggt cgacggacag gccgccggtg gccgcatccc
     2581 aggccagcag gagtcccggt acgagggcgc cgacatgacc ccccgtccgc acgaggagag
     2641 aagacgccga ccggcgccgt tgtcttcgcg gcgcggccgt ctggggcggg gacgtcatcg
     2701 gaggcaggac tagaagtcct tgcgcaggtc catgccctcg tacagggacg ccacctcgtc
     2761 gtatccgttg aacatgagtg tgtctcggcg cccgaactcg ccgatgcggc gttcggcctt
     2821 ctgcgaccag cgagggtggt ccaccgtggg gttcacgttg gcgtagaagc cgtactcacg
     2881 gggcgacgac gccatccaga agctcaccgg catctcgcgc acgagttcga tcttgactat
     2941 cgacttaatc gacttgaacc catacttcca gggcacgacc agacggatcg gcgccccgtt
     3001 ctggggaagc aggcgcttgc cgtagaggcc ggtggagagc agggtgagat cgtgcgtggc
     3061 ttcgtcgagt cgcaggccct cgatgtaggg ccagtcgtag ctgtatttct tctggccggg
     3121 gagatgttcg ggatcatcga tcgactggaa acgcacaaaa cgggcttcgg aagtagggcc
     3181 gaccatctcc aggagcgccc ccagggggat tccgagccag gggatgacca tcgaccagcc
     3241 ctcgacgcac cgcatgcgat agatgcgttc ttcctcgggg agtagagcca ggagatccgc
     3301 gatggtgaac gtgcgcgggg agtcgaccag accacccacc tcgagggtcc aaggtgtgct
     3361 gacgaagtcg gcggctttct cggccacctc ctccttgtca actgtaaact cgtagaagtt
     3421 gttgtacgtg gtgacggcct cataggaggt gaggccctgc ccgagctcgt ccgtggcggc
     3481 tccagtggag ccgggtgtgc ccacagtggt tgaggctccc tcgccggacg tgcccgtgtc
     3541 gccccccacc tcgcacccgg ccagcacggc tgctccggcc gtggcggcga cgccgaccat
     3601 gaaactgcgg cggctcaggt agatgtgctc gggggtgatc tcgctggggt ctattcggtt
     3661 catactgttc tatcccccgc ggattgccgg gctacgcccc gttcagtttc gcacgcccgc
     3721 gcaggcggtt acgccgtctg gatttgggga aagcatcacc gcgaccaggt tcgcgatcga
     3781 gaggaccggg aaccgccggc cagaaggaag tgcgttcatg gccaatcgtc tcatcggtga
     3841 gaccagtccc tatctgcttc aacacgccca caaccccgtg gactggtacc cctggggcga
     3901 ggaggcgttg gccgccgccc gtagccagcg ccgccctgtc ttcctgagca tagggtacgc
     3961 ggcctgtcac tggtgtcacg tcatggagcg ggaatcgttc gaggatgtgg agacggccgc
     4021 gctcatgaac gagcgcttca tcaacatcaa ggtggaccgt gaagagcggc ccgacctcga
     4081 ctccatctac atgcgggccg tcactgtcct caccggccgg ggcggctggc ccatgagcgt
     4141 gtggatcact cccgagggcg cacctttctt cgggggcacc tacttccccg acacgccgcg
     4201 tcacgacatc ccctcgttcg cccaggttct cgaggcgttg gcggacgcgt gggagagccg
     4261 tccggacgag atagctgcct ccgcggcaaa gctggcaggc aagttgaggg aggacgcagg
     4321 gctgccggtg ctgcggcgta ccatggacaa aggtgacgac gacgcacgcg ttgcggcagg
     4381 ggccgcgtcg gccgacctgg tgccggcgcc ggccggcctc gaccccgaga ccggcgcgga
     4441 ggcttccctt gccctcatct ccggtttcga cgccgcgaac ggggggtggg gcgaggcgcc
     4501 caagttccct cagccgatgg cgctcgagtt cttgttgcgt cggtgggtgc gtcacggcga
     4561 cgagcacgcc cgcatcctcg tggagcggtc gttggacttc atggccgtcg gcggtatcta
     4621 cgatcagctg ggtgggggct tccaccgcta ctcggtggat gcggagtggc tggtgccgca
     4681 cttcgagaag atgctctacg acaactccca gctggcccgg gtgtacaccc atgcctggca
     4741 ggcccttggc cacgagcgct accggcaggt ggctgaggag actctcgact acgtgctccg
     4801 agagatgacc catcctgacg gcgggttctt cagcacccag gatgccgact ccgagggggt
     4861 ggagggcaag ttcttcgtgt ggacccccga cgagatcgag gcattgctcg acgccgacgc
     4921 cgcagacgcc ggcggcgatg cggccgacca gtgtctcgga gccaccgacc ggcacagcgg
     4981 cgaagccggc atcgacgccg cccgtctgcg ccgccacgcc gacgccgccc tcttcatgga
     5041 ggcctatggg gtcgagccag gaggcaactt cgagggcgcg tcgatcctct cccgagtgcg
     5101 caccgacgaa gacctcgccc gcgccgaggg cgactctgcg gccacggtgg cggcgcggct
     5161 cgccgccgcc cgcgcccgcc tactgacggc gcgggaagag cgggcgcacc ccgcgcgcga
     5221 cgagaagatc ctcgccgcgt ggaacggcct catgatcgcc gccttcgcgg atgccgcggc
     5281 agccttcggg cgctccgact atctgcttgc cgccgagcgt gccgccgcct tcgtgctcca
     5341 gcggctgcgc ggtcccgacg ggcgtctgat gcgctcctgg cgggacggaa gcacccgcca
     5401 caacggctat ctcgaggatc acgctcacgt gatcgaaggt ctgctcgccc tctaccaggc
     5461 cgacttcgat ccccggtggt tcgtggccgc ccgcgacctg gccgacgaga tgctcgcctc
     5521 cttcggatct gcggagagcg gcttctacga cacgcgcgac gaccacgagt ctctcttcct
     5581 gcgtccccag gactaccagg acaacgccgt gccctcgggc aatgccatgg cggcgacggt
     5641 gttgctcaaa ctccgggcct acaccggcga ggccaggtat gcggcgcccg cggagcgcac
     5701 gctcttaggc ttggagccgt tgctctcccg tcaccctctg gccttcggac aggggctcat
     5761 cgccttcgac ctagcggtgg gggagggcgg cgaggtgg
//
LOCUS       DASRMJ010000042        19833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_328357, whole genome shotgun sequence.
ACCESSION   DASRMJ010000042 DASRMJ010000000
VERSION     DASRMJ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 19833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19833
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328357"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFD74_01225"
     CDS             <1..917
                     /locus_tag="VFD74_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZK48207.1"
                     /translation="QTCRGSQSTETGGNMGAEDTLRLDRTNAAIVVDSTADLSEGIAA
                     DPNLSMVPLNVHFGEETFKDWVDIKPNEFYQRLAKAERLPTTSQPPVGAFIQEYKRLR
                     TQFERIFSVHLSAKLSGTFESATLAQREVSGVTVIDSEVASMAVALLARQLLGLLDRG
                     TTEAEILEYVDRFRRESGRLFLLGSLEYLQKGGRIGRASGLAGSLLNIKPLLTFQDGI
                     VHPYKKVRGDQKALAAMKDYFLDNTHPGRKVAVAIAHGDSPERIDSVMELLSTTDRDF
                     RVVLKGQVGSVIGTYAGPEAVALFFVEE"
     gene            914..3100
                     /gene="recG"
                     /locus_tag="VFD74_01230"
     CDS             914..3100
                     /gene="recG"
                     /locus_tag="VFD74_01230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZK48208.1"
                     /translation="MSRRGGGIHFPFGAPASAFAEAPALPPSSKRRARPARLHRRLDT
                     LPGVGPVRAKRLAGLGLESIEDLLLYTPSRHEAPARLATVASLRDGEEATLRVIVQSF
                     SVRSAQRRGLTVLEALIGDQTASVPAVWYNQAYLADVFAGKPEILVRGALRRSRGGYS
                     FLVSSHEIIREDGREEGLHTVGLIPVYPATGDVSVRSLRSLLGAVRSEAEHVVDPLPA
                     RLVSERGYPSRREALLSAHFPRSLAEAAKSRERLAYEELLFLQVALLRHRRHEEAERP
                     ALPLPPAEQLASTFIERLPFSPTRAQRRVMAEIDGDLQSSTPMRRLLQGDVGSGKTIV
                     AAYSLLRAVEAGGQGVVMVPTEVLADQHADRLGRQLARLGVETLLLKGSQSARDRRDA
                     LKRISSGDPLVAVGTQALIQEGVDLSSVRVVVIDEQHRFGVRQRMALVTPPAGRPWPH
                     TLHMTATPIPRTLGLTLYGDLDVSVIDELPPGRKPVDTRVVELEHRGRMWDFVREKVD
                     QGRQAYVVCPLVEESESLQAASAITTFKELSRADLRGLRLALIHGQMKSDRKHEIMRA
                     FERGEYDVLVTTTVIEVGIDVPNATVMVIEDARRFGLSQLHQLRGRIARGAEAGHCLV
                     GAEGCDEESRARLELFCRTTDGFELAEADLRARGEGQVFGERQSGWSDLKVARLLRDQ
                     RLLMSAREEARTMLAADPELQDPRHTLLGEAAEDRFGAATHWLDHA"
     gene            3093..3659
                     /gene="rsmD"
                     /locus_tag="VFD74_01235"
     CDS             3093..3659
                     /gene="rsmD"
                     /locus_tag="VFD74_01235"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZK48209.1"
                     /translation="MPRRPGEIRIIAGSHKGHRLKVAAGDVVRPTKDRVREAVFSTLG
                     DLTNTRVLDLFAGSGALGLEALSRGSAHATFVEQDRTVAGILKRNITSLGLDARAEVL
                     ETPYDSALRRLLQRGGVFDLLFLDPPYTMLAQVDEAVATVLPQLLGPGGLVIVEGPRR
                     AQARLGLPVVFQRRYGNTLISIYGEEQH"
     gene            3661..4152
                     /gene="coaD"
                     /locus_tag="VFD74_01240"
     CDS             3661..4152
                     /gene="coaD"
                     /locus_tag="VFD74_01240"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZK48210.1"
                     /translation="MRTALCPGTYDPVTVGHLDIISRCAKNFDMVFVAVVNDAFRKQP
                     LFSAEERVSFLEESTRHIANVTVVVLEGLVVEKARQVGADSLVKGLRAVTDFEYEFQM
                     AQLNKKLDPDLETVYLMASPEYSFLSSSGVKEIARFGGRVDDLVPPMVAERLAQVYSH
                     PEA"
     gene            4155..4613
                     /locus_tag="VFD74_01245"
     CDS             4155..4613
                     /locus_tag="VFD74_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZK48211.1"
                     /translation="MDVLVLIDKLDDLVHTASTLPMTDKVMIDRDQIYDLLDQMRATI
                     PEEIKQARWIVKERQEMLAEAKEESERLLSEAREESARLASEQEVVKRAERQAEEIIR
                     DAEVRNREIRFGAEDYADEVLGALETSLDKFLSAVQRGRERLQGRTQREE"
     gene            4641..5177
                     /locus_tag="VFD74_01250"
     CDS             4641..5177
                     /locus_tag="VFD74_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HZK48212.1"
                     /translation="MARSGYLLDLRGLSLRPGELVEKTYQLGIAPIVFGGMPSGVVLD
                     ESGVRVQANRIAGGHLVRVGLGATVYAPCARCLVEVAVHVSADQEEFVPQHPEEWEEA
                     DLSPFIEDLIVDVDGLARETLVLALPAKVLCDEACAGLCPSCGNPSGSAACGCARTPG
                     DERWARLADLTLSDSEGE"
     gene            5291..5470
                     /gene="rpmF"
                     /locus_tag="VFD74_01255"
     CDS             5291..5470
                     /gene="rpmF"
                     /locus_tag="VFD74_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZK48213.1"
                     /translation="MAVPKKKTSKARRDSRRATHSIPGPTVGECPQCHSPRLNHRVCP
                     NCGTYRGKQVVEVEE"
     gene            5586..5873
                     /locus_tag="VFD74_01260"
     CDS             5586..5873
                     /locus_tag="VFD74_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48214.1"
                     /translation="MGESTIFHCESCGYESPQIRWGVSVVDPRRRFMPAHCMHCKAYV
                     EVDLTGADVLVDEFQCPTCGHEVFFVERSDSYGCPQCGRPGIRLRQGPSYW"
     gene            5952..6197
                     /locus_tag="VFD74_01265"
     CDS             5952..6197
                     /locus_tag="VFD74_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48215.1"
                     /translation="MQLIVQEGRAVLSLVTPIQVNDDSMRSQTVSEFSENVKAYYRDF
                     ELVRVEGRDGQLEIQPANVASIFWVEKGRRYQLKTNP"
     gene            6476..8557
                     /gene="fusA"
                     /locus_tag="VFD74_01270"
     CDS             6476..8557
                     /gene="fusA"
                     /locus_tag="VFD74_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZK48216.1"
                     /translation="MAQVDPAKIRNVAVVGHRGSGKTSLVEAILYTAGAKNRLGSVVD
                     GTTTLDHDEDEIKRQMSISAAMAHVEWDKRKINVVDTPGEASFINEAMATLTVVEGAL
                     IVVNAVAKVEVQTERLWRRALDLGVARLVAVNMMDRERADFEEVLTALADRFGDGVVP
                     VALPIGKEQSFSGLVDLVHMKAYTYADSSGKAKEIPIPAELLEAATTARELLVERVAE
                     SADSLIEKFLEGEELTLEEILEALKAGAIEGRLSPVIPVSATKNIGIDRLLDMVEFGL
                     PSPARVGARRVPLADGSGDVEVSPDPNGPAALFVFKTISDQFSGRINLAKVLSGHIAS
                     DSHLVNPRTGDKERTGNILTMQGKESKGIEEASAGDIIALGKLKDTSTGDTLADASLA
                     VRLPSFEFPPPAISFAITAKTKGEEEKVANALKRLGDEDPAMQFRFDAQTHEMIIAGT
                     SQMHVEVLLDKMKRRFGAEVDLHPPSVPYRETIRKTSTAQGRHKKQTGGRGQFGDCWI
                     EIQPLPRGGGFEFEDAIVGGSIPRGFIPAVEKGVIETMDRGVIAGYSVVDVKVKLFDG
                     SYHTVDSSEMAFKIAGSLAFAKAMEGAAPVLLEPVMNVEVIVPEANMGDIMGDLSSRR
                     GRPQGSESMGEMHAIRAQVPLAEMLTYAPQLRSMTGGRGSYTMELDHYDEVPAHLTEK
                     IVEDAKAARGE"
     gene            8762..9625
                     /locus_tag="VFD74_01275"
     CDS             8762..9625
                     /locus_tag="VFD74_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HZK48217.1"
                     /translation="MDDPKPKRVTDLLQEGFDSFSRSQKAIARYIIDHLEETAYLSAE
                     ELARKGNTSSSTVVRFAQSLGFSGFPDLQKATRDEHRLGAASRPVVHDDQLLFLVEED
                     NLTRVLRTDILSLEETLAKNTLGRFNQAAEMISTSRQLMLVGMNEASIVVRHAAYVFD
                     LLGFTTHLVVDSSEANVAKLVRLGPDDAVLAVGFRRAHPLTISFLEKAVTAGVRTVSI
                     TDNSLSEIAGKASVTLYADIDSTFFAHSLVGPLSLVTALASATYARDRDACDARIKLV
                     REHVGESGWLR"
     gene            9873..10625
                     /locus_tag="VFD74_01280"
     CDS             9873..10625
                     /locus_tag="VFD74_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZK48218.1"
                     /translation="MSGHSKWAGIKHKKALVDAKRGKEFGKFSRAITVAAREGGGDPE
                     HNAALATAIQKAKDANMPNDNIDRAVKKGTGESADAAAYMHLTYEGYGPNGVAVYVMV
                     LTDNKNRSAADVRHIFDRSGGNLGTDGSVSWMFERKGVVYVDAAGRDEDEVMMVAIEA
                     GAEDMIVDGDSLEIRTEPFDFMAVRHALEAAGIPVASAQLTMIPKNTVALDEADAKKT
                     FRLLDALEDNDDVQEVFANFDITDEVMEALAG"
     gene            10662..11213
                     /gene="ruvC"
                     /locus_tag="VFD74_01285"
     CDS             10662..11213
                     /gene="ruvC"
                     /locus_tag="VFD74_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZK48219.1"
                     /translation="MPDTGRIILGVDPGTAFTGYGVVSQNGNRLETVAFGVVRTSKDL
                     PLHERLDLIFEGIGDLVRNYRPTEAAVESLFFNVNVRTALAVGQARGVALLACTRGGC
                     DVFEYTPQEVKQAVVGYGKAEKSQVQEMVRVLLKMNDIPKPDHAADALAIAICHAHGR
                     GLRALADRESAGRRRPLGAGGAA"
     gene            11210..11794
                     /gene="ruvA"
                     /locus_tag="VFD74_01290"
     CDS             11210..11794
                     /gene="ruvA"
                     /locus_tag="VFD74_01290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZK48220.1"
                     /translation="MIDRLTGRVAALEADAAVLEVSGVGFRVEMSRTSLRALPEEGNA
                     ASLFTHLHVREESLQLFGFSTEEERRLFLHLISVSKIGPRLALAALSIRKPRDLRRAI
                     AAGDVALFQSVPGIGKKTAERLILELREKMAEIEGPGTLIGRGSPDEAGNLAVARAAL
                     AELGIGAAESEALMRDTDPDTPVDEIVRQALRRR"
     gene            11797..12867
                     /gene="ruvB"
                     /locus_tag="VFD74_01295"
     CDS             11797..12867
                     /gene="ruvB"
                     /locus_tag="VFD74_01295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZK48221.1"
                     /translation="MDHFTPAEHGAEPGERDDRLADPEELVDDRELEGSLRPRSLAGF
                     IGQPVLREQLSIFIEAAKSRGEPLDHVLLAGPPGLGKTTLAAIIALEMGVHIVTTSGP
                     ALEKKADMAAILTNLEEGDVLFIDEIHRLNRVIEEILYPAMEDFEIDIIIGQGPSARS
                     IRLDLPHFTLIGATTRSGLITTPLRDRFGFSHRLDYYSEADLASIIARSADILCVGID
                     PEATHELAVRSRGTPRIANRLLKRVRDFAQVRHAGHVSAEVGKEALRLFQVDDEGLDR
                     VDREILNLILRKFDGGPVGLSTLAVAIGEEADTIEDVYEPYLLQRGFLMRTPRGRVLT
                     RLGYLHCGAQPPDGGPPTLFGG"
     gene            12864..13397
                     /locus_tag="VFD74_01300"
     CDS             12864..13397
                     /locus_tag="VFD74_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HZK48222.1"
                     /translation="MRLLLHMCCGPCATATLDHWAGDGADVTGFFHNPNIHPLLEFRR
                     RVTGVRDLALARRVHVIEDLAYDPSAWFREIVGEDASGRCGRCIASRLRATARVAAAS
                     GYDAFSTTLAISPWQDHEAIREGGGSAQQEHHVEFIYRDLRAAYPDSRRAAREAGLYR
                     QKYCGCLLSEWERYREK"
     gene            13429..14574
                     /gene="queA"
                     /locus_tag="VFD74_01305"
     CDS             13429..14574
                     /gene="queA"
                     /locus_tag="VFD74_01305"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZK48223.1"
                     /translation="MRLDELDFDFREDLIAQHPSEPRDACRLLVADPRTGSISHHDFK
                     HIVARARPGDVLVVNESKVFPARTWARKETGGRVELLFLRRLPDPVPTDGASDPAPPA
                     GSSDADGADDTAEGGLPTETWEVLARPSARLRPGRNLTLDTGDTLELAALLGDGRWAL
                     KRRGNPGVLELLELCGTMPLPPYIHEPLRRAGDYQTVYAAATGSAAAPTAGLHFTTEV
                     LGTLQAAGVDVERVTLHVGVDTFRPLTELRIEDHAIHSEWYRVEPDAVARIDRARAAG
                     RRVIAVGTTAVRVLETLYEGSAPGGPSSGALEGSTRVYITPGYRFRAVDALITNFHLP
                     RTSLLALVMAFGGVEFVRECYETAVREGYRFFSFGDAMFLEARAGEA"
     gene            14577..15734
                     /gene="tgt"
                     /locus_tag="VFD74_01310"
     CDS             14577..15734
                     /gene="tgt"
                     /locus_tag="VFD74_01310"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZK48224.1"
                     /translation="MGPSLPTFPFELTRTDGAARAGILSTPRGEVPTPCFMPVGTKGT
                     VKAISPHRLREIGARIVLANTYHLALRPGSDTVAELGGLHAFMGWTGPILTDSGGFQV
                     FSLKDTARVDDSGVTFRSVYDGSLHTFTPERAMSEQNRLGADMVMCFDECAAAGADLA
                     ALEGAVTRTTEWARRCCDAHAEVGGVGTDGPQMLFGIVQGGIDPGLRRRSAEGLLELG
                     FPAYAVGGLSVGEDRGATLETTALTAQLLPAERPRYFMGIGDPVGLLEVIDRGIDMFD
                     CVLPTRIARMGTAFTSHGRVNLRNAQHAQSRGPLDPDCPCRACSQFPLGALRHFVMQK
                     EILGLQLLTEHNLLFLFRLVAGARNAILEGRYGAFKKGVLESFVPAAPDSR"
     gene            15805..16131
                     /gene="yajC"
                     /locus_tag="VFD74_01315"
     CDS             15805..16131
                     /gene="yajC"
                     /locus_tag="VFD74_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZK48225.1"
                     /translation="MGSFGLLALYVVGFIAIFYFLAIRPQQRQRKAHDALVSSVKRGD
                     HVISVGGLHGKVKRTEDTVVVIEVAKGVDIRLSRKALAEVVSQAAQEQAAQQIEAAEP
                     EESEEA"
     gene            16284..17669
                     /gene="secD"
                     /locus_tag="VFD74_01320"
     CDS             16284..17669
                     /gene="secD"
                     /locus_tag="VFD74_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZK48226.1"
                     /translation="MQRNVVILAVVAVLLCVAAYFIYPPGSSTRLGLDLQGGLAVILE
                     AQDSARAPRTDDGMKQTISIINDRINRLGVTEPEVQRQGEWKISVQLPGIENKQEALA
                     IIGKTAVLEFYDVKDFGAAYASEAEALKAAGVDSEAQLPADTRLVRRPSRDGTLPDRF
                     YIVTAIPAVTGTGLSGASVGFDNNNQPKIDMQFKSDGAQAFAEVTQALADRAQVTGQD
                     QLLAIVLDGVVQSAPRVAERIDGGRAEITGRFTLDEAKNLVLVLQTGALPIELKVIDE
                     RSIGATLGKASLTQALWAGVVGLILVAVFMVAYYRLLGLVADLTLLIYGVLFWGILNS
                     IHATLTLPGIAGMILTLGMAVDANVIIFARIREEVHAGKTVRTAVEAGFRKALSAILD
                     ANVTTLITAVVLFWAASGGVRGFALTLGIGVALSMFTAIVVTRALLALLSGLKIFKNP
                     TLTGLSVETER"
     gene            17670..18563
                     /gene="secF"
                     /locus_tag="VFD74_01325"
     CDS             17670..18563
                     /gene="secF"
                     /locus_tag="VFD74_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZK48227.1"
                     /translation="MTVYPFMKYKRWSVILLGAVTLFAAGAILVLGLRLGIEFNGGVR
                     LEVQLKQDAATSDVRTIVSEAGIADTVVQPVGSKGFVITAAEMSDAQVASVIKALGDT
                     YGAEESATGQERVGASFGAETGQKALIAILVAVAVIILYITWRFEYKFAFPAIVALVH
                     DVALTLGVYAVTGRLVTTATVAAVLTIVGYSVNDTIIVFDRIRENQAFMKKESYGSMV
                     DLSIRQTLARSLNTALTTLLPIATILLFGGSTLKDFAFALFIGIASGAYSSIFVASPL
                     LAYWKEREPRYRRRAMADKTA"
     gene            18683..19078
                     /locus_tag="VFD74_01330"
     CDS             18683..19078
                     /locus_tag="VFD74_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48228.1"
                     /translation="MTEQHDDRRSEDQAVEATGLRDIIERTLLVGLGAASLTKERLQK
                     VADEFVRRGQLSAEDGRALVEGLAARSREEARSALKSADSSVQTVFRELGIASRRELE
                     ELDFKLRQIEHRLALLERESDSSSAASTD"
     gene            19127..>19833
                     /locus_tag="VFD74_01335"
     CDS             19127..>19833
                     /locus_tag="VFD74_01335"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HZK48229.1"
                     /translation="MTQRYQGLRGRARNLERLRQISEVAVRHGLGYFFERHNLWHILS
                     VRRREVQPPPSQLGRHIREFLEELGPSFVKFGQLLSTRSDLLPHEIVSELVKLQDSVP
                     AFPVEEARAVLREDLGLTAERLFESFEPVPIAAASIGQVHRAVLPGGMQVVVKVQRPR
                     AMGQIERDIDLLYQIAELLSEHFGERLVVDPVKVVDEFAHGISAELDYTLEGRNAERF
                     AANFSAEPAVAIPRVFWT"
BASE COUNT         3566 a         6338 c         6511 g         3418 t
ORIGIN      
        1 ggcagacatg ccgcgggagc caaagcaccg agacaggggg gaacatggga gctgaggaca
       61 cattgcgact ggacaggacg aatgcggcga tcgtcgtgga ttccacagcc gatctgagcg
      121 aaggcatcgc cgccgacccg aacctttcga tggtgccgct caacgtgcac ttcggagagg
      181 agaccttcaa ggactgggtg gatatcaagc cgaacgagtt ctaccagaga ctcgccaagg
      241 ccgaacgcct tccaacgact tcgcagccgc ccgtaggcgc cttcatccag gagtacaagc
      301 gcttgaggac gcagtttgag cgcatcttct cggtgcacct cagcgcgaag ctcagcggga
      361 ccttcgagag tgccaccttg gctcagcgag aggtgtccgg ggtcaccgtg atcgactccg
      421 aggtcgccag catggctgtg gcgctcctcg cccgccaact gctgggcctt ctcgatcgcg
      481 gcactaccga agcggagatc ctggagtacg tggatcgctt ccggcgcgaa tccggacgac
      541 tcttcctgct cggcagcctc gagtaccttc agaagggcgg acgcatcggt cgcgccagcg
      601 gacttgccgg gagccttctc aacatcaagc ccctgctcac cttccaggac gggatcgttc
      661 acccctacaa gaaggtccgt ggggatcaga aggccctcgc tgcgatgaaa gactacttcc
      721 ttgataacac gcaccccggg cgcaaggtgg cggtcgcgat agcccatggc gactcccccg
      781 aacgcatcga ctccgtcatg gagcttctct ccaccaccga ccgcgacttc cgagtggtcc
      841 tcaaagggca agtcggctcg gtgatcggaa cctatgccgg acctgaagcg gtagcgctgt
      901 tcttcgttga ggaatgagtc gacggggggg tggcatccac ttccccttcg gtgcgccggc
      961 ctccgcgttc gcggaggcgc cggcgttgcc tccatcctcc aagcgacgag cgcgaccggc
     1021 tcgtctccac cggcgtcttg acacccttcc cggcgtaggt ccggtcagag cgaagcgtct
     1081 ggccggattg ggcctcgagt cgatcgagga tctcctcctc tacacgccct cccgccacga
     1141 ggctcccgcg cgcttggcga cggtcgcatc cctccgcgac ggagaggaag cgacgttgcg
     1201 tgtgatcgtg cagtcattca gtgtccgctc cgcccaacga cgaggcttga cggtcctcga
     1261 ggctctgatc ggcgaccaga ccgccagcgt gcccgcggtg tggtacaacc aggcctatct
     1321 ggccgacgtc ttcgccggca agcccgagat cctcgtgcgc ggggccttgc ggcgcagccg
     1381 aggcggctac tcgttcctgg tgagcagcca cgaaatcatc cgtgaggacg gccgcgagga
     1441 aggcctccac acggtggggt tgatccccgt gtatccggcc accggcgacg tgagcgtccg
     1501 ttcccttcgc tctctgctcg gggccgtgcg ttcggaagcg gagcacgtgg tggacccgct
     1561 gccggcacgg ctggtgtcgg agcgcggcta cccgtcgcgt cgggaggcgc tgctgtcagc
     1621 gcactttccg cgctcgttgg ccgaggccgc caagagtcga gagcgtcttg cgtacgagga
     1681 gctgctgttc ctgcaggtcg cgctcctacg tcaccgcagg cacgaagagg cggagcggcc
     1741 ggcgctgccg ttaccgcctg ccgagcagct tgcgtcgacc ttcatcgaac ggctgccgtt
     1801 ctcgcccacc cgagcgcagc gtcgcgtgat ggccgagata gacggcgacc tccagtcgtc
     1861 aacccccatg cggcgcctgc ttcagggaga cgtggggtcg gggaagacca tcgtagccgc
     1921 ctactcgctg ctgcgggccg tggaagcagg gggccaaggg gtggtcatgg tccccaccga
     1981 agtcctggcc gaccaacacg ccgatcggct ggggcggcaa ctggcgcgcc ttggcgtaga
     2041 gaccctgctg ctgaagggca gccaatcggc ccgcgatcgg cgcgacgcgc tgaagaggat
     2101 ctcgagcggg gacccgctcg tggcggtagg gactcaggct ctgatccagg aaggtgtgga
     2161 tctctcgtct gtgcgggtgg tggtcatcga tgagcagcac cgtttcgggg tgcggcagcg
     2221 catggctctg gtgaccccgc cggccggccg cccgtggccc cacactctgc acatgacggc
     2281 gacgccgatc ccgcgcacac tcggccttac gctgtatgga gatctcgacg tcagcgtcat
     2341 agacgagctg cctcccggca ggaagccggt ggacactcgg gtcgtcgaac tcgagcacag
     2401 ggggcggatg tgggacttcg tcagggagaa ggtggaccag ggacggcagg cgtatgtcgt
     2461 ctgccctctt gtggaagaga gcgaatcact tcaggccgca tcggctatca caacgttcaa
     2521 agagctctcc cgagcggacc tgcgcggtct ccgtctcgcc ttgatacatg ggcagatgaa
     2581 gagcgaccgg aagcatgaga tcatgcgcgc gttcgaacga ggggagtacg atgtcttggt
     2641 cacaacaacg gtcatagaag tggggatcga cgtgccaaac gccacggtca tggtcataga
     2701 ggatgccagg cgtttcggcc tgtcccaact ccatcagcta cggggccgca tcgcccgggg
     2761 agccgaggct gggcactgcc tggtgggcgc ggagggctgc gatgaagagt cgcgggcgcg
     2821 actcgaactc ttctgccgga ccaccgacgg gttcgagctg gcggaagccg atctgcgggc
     2881 ccgcggcgaa ggacaggtgt tcggcgaacg ccagtcgggt tggagcgatc tcaaggtggc
     2941 acgactcctc cgggaccagc ggctccttat gagcgcacga gaagaggcga ggaccatgct
     3001 ggccgccgac ccggaactcc aggatcctcg ccacacgctc ctgggtgaag cggcggagga
     3061 ccgcttcggg gccgccaccc attggctgga tcatgcctag gcggccggga gagatccgga
     3121 tcatcgccgg gagtcacaag ggtcaccgct tgaaagtggc ggccggagat gtggtgcgtc
     3181 ccaccaagga tcgggtgcgg gaggccgtct tctcgactct gggcgacctc acgaacacgc
     3241 gtgtcctcga ccttttcgcg gggtccgggg cactcggact cgaggccctc tcgcgcggct
     3301 ccgcccacgc taccttcgta gagcaggacc gtacggtcgc gggcatcctc aagaggaaca
     3361 tcacctcgtt gggcctggat gctcgcgccg aggttctcga aaccccgtac gattcggctc
     3421 tccgccgtct cctgcaacgt ggaggggtct tcgatttgct ctttctggac cctccttata
     3481 caatgctggc gcaggttgac gaggccgtcg cgaccgtgct gccccagctg ctcggacccg
     3541 ggggcctcgt gattgtggaa ggacctcgca gggcgcaggc acggctcgga ctcccggtag
     3601 tgttccaaag gcgctacggc aacactctca tctcgatcta tggcgaggag caacactgac
     3661 gtgagaacag cgctctgccc cggcacgtac gatcccgtga ctgtgggcca cctcgacatc
     3721 atctctcggt gcgccaagaa cttcgacatg gtcttcgtgg cggtggtcaa cgacgctttc
     3781 aggaagcagc ctctcttctc cgcggaggaa cgggtctcgt tcttagagga gtccaccagg
     3841 catattgcga acgttaccgt ggtggtgctg gaggggttgg tggtggagaa ggcgaggcag
     3901 gtgggcgccg actctctggt caagggtctt cgagcagtga ccgacttcga gtacgagttc
     3961 cagatggcac agttgaacaa gaagttggac cccgatctgg agaccgtgta cctaatggca
     4021 tcgcccgagt atagtttctt gagttcgagc ggcgtcaagg agatagcgag gttcggcggt
     4081 cgtgtcgatg acctcgtgcc gccgatggtc gccgagcggc tcgcccaagt gtattcacac
     4141 ccggaggcct gaagatggat gtcctcgttc tgatcgacaa gctcgacgat ctggtccaca
     4201 ccgcgagcac tttgccgatg accgacaagg ttatgatcga tcgtgatcag atctacgatc
     4261 tgctggatca gatgcgtgcc accatccccg aggagatcaa gcaggcgcgg tggatcgtca
     4321 aggagcgtca ggagatgctc gccgaggcca aggaggagtc ggagcgtctg ctgagtgagg
     4381 cccgcgagga gtcggcgcgc ctcgcgagcg agcaggaagt ggtgaagcgc gccgagaggc
     4441 aggccgagga gatcattcgc gacgccgaag tgcgcaaccg cgagatccgc ttcggcgctg
     4501 aagactatgc ggacgaggtc ctcggcgccc tggagaccag tctggacaag ttcctctccg
     4561 cggtccagcg cggccgagag cgtctgcagg gtcgaacgca gcgagaggaa tagatcactc
     4621 cgtccggcta cacgccacct atggcaagga gtggatatct gctcgatctg cggggactct
     4681 ccctccggcc gggtgagctc gtcgagaaga cctaccaact cgggatcgct cccatcgtat
     4741 ttggcgggat gccctccgga gtggtgctcg acgagagcgg cgtccgtgtg caggccaaca
     4801 ggattgccgg cggccacctt gttcgggtgg gtctgggggc gacggtgtac gctccctgcg
     4861 caaggtgcct tgtcgaggtg gccgtgcacg tctccgccga tcaggaggag ttcgtccctc
     4921 agcatccgga agagtgggag gaggccgatc tctctccgtt catcgaggac ctgatcgtgg
     4981 atgtggacgg gctcgctcga gaaaccctcg tgcttgcgtt gccggcgaag gtcctttgcg
     5041 acgaggcgtg tgcgggcctc tgcccaagtt gtggcaaccc gtcaggctcg gcggcctgtg
     5101 gatgcgcccg gacgcccggt gacgaacggt gggcgcggct ggccgatctc actctgagcg
     5161 actcagaagg tgagtagaac gcgcagatca ggcttcggcg agccgacccg gtggggcctt
     5221 cgccttgaaa accgggccga tgtgcgctaa taatccctga cttcgaacaa aggtagagaa
     5281 agggtccaac gtggccgttc ccaagaagaa gacgtccaag gcacggcgtg acagtcgtcg
     5341 cgcaacgcac agcatccccg ggcccaccgt gggtgagtgt cctcagtgtc acagtccccg
     5401 cctgaaccat cgcgtctgcc cgaactgcgg cacttaccgc ggcaagcagg tggtagaggt
     5461 cgaggaatag cactccgctc ctcccggagc gcggccgtcg tgggggcgcg cccgggtccg
     5521 ccgctcgcac tcgcctgagt gggagaaggc ggtggcggcg gacagatcag agagaggcaa
     5581 catcgttggg tgagtcgacc atcttccact gcgaatcgtg tggctacgag tcaccacaga
     5641 tccgctgggg tgtgagcgtc gtcgatcccc ggcggcgctt catgccagcg cactgcatgc
     5701 actgcaaggc ctacgtcgag gtcgatctga ctggggccga cgtactcgtc gacgaattcc
     5761 agtgtcccac gtgcggccac gaggtcttct tcgtggagcg ttccgactcg tacggatgtc
     5821 ctcagtgcgg ccgcccgggg atacggctgc gacaaggtcc gtcttactgg taggctgtcg
     5881 ctgacttggg gcgggaatct tcgctcgtca accgatggcc ttcaggtgcc cactcgggag
     5941 gttgaagaag catgcagctg atcgtccaag aaggccgtgc cgtgctgagc ctggtcacac
     6001 ccatccaggt gaacgacgat tcgatgcgta gccaaaccgt gtcggagttc tccgagaacg
     6061 tcaaggccta ctaccgcgac ttcgaactcg tgcgcgtcga ggggcgcgat ggacaactag
     6121 agatccagcc cgcgaacgtg gcaagcatct tctgggtgga gaagggtcgg cgctaccaac
     6181 tgaagaccaa cccgtagggc gagccgaccg tcgacgccag aggcccttat catccgtagc
     6241 cgcgttcggt agaatatgcc tcctgtcaac ccgccgcgcg cgaatgcggc gtaagcactg
     6301 ggagcgaggt gagcaggcct atgggccgtc acagtatcgt cagtgtacga atctcatagc
     6361 ggctcttgag gacgccgccc ttcggggcag cgtcctttgt catgtatggg cccgggtgca
     6421 ccacggggcg gagcacacgg ttcagcgaga cacccactac gtatgaggag gactgatggc
     6481 tcaggtagac ccggccaaga tccggaatgt cgcggtagtc ggacaccgcg gctcggggaa
     6541 gacctcgctg gtggaagcca ttctgtacac cgcgggcgcg aagaaccgac tgggcagcgt
     6601 tgtcgacggc acaaccacgc tcgaccacga tgaggacgag atcaagcggc agatgagcat
     6661 ctccgcggcc atggcccatg tcgaatggga caagcgcaag atcaacgtgg tggacactcc
     6721 gggcgaggcc tccttcatca acgaagccat ggccactctc acggtggtcg agggcgcctt
     6781 gatcgtggtc aacgcagtgg cgaaggtcga ggtgcagacc gaacgcctgt ggcgcagagc
     6841 gctggacttg ggcgtagccc gactggtagc cgtcaacatg atggaccgtg aacgcgcgga
     6901 cttcgaggag gtccttaccg ccttggccga tcgcttcggc gatggcgtgg tcccggtggc
     6961 cctcccgatc ggcaaggaac agagcttctc cggacttgtg gacctcgtac acatgaaggc
     7021 gtatacctac gccgactcca gcggcaaggc gaaggagata ccgatccccg cggaactgtt
     7081 ggaagcagcc acgacagccc gcgaactcct cgtggagcgg gttgctgagt ccgccgactc
     7141 cctcatcgag aagttcctgg aaggggagga actcacgctc gaggagatac tcgaggccct
     7201 caaggccgga gccatcgagg ggcggctctc gccggtcatc cccgtgagcg caaccaagaa
     7261 catcggcatc gatcgcctcc tcgacatggt cgagttcggg ttgccgtcgc ctgcgcgtgt
     7321 gggcgccagg cgcgttcccc tggccgacgg ctcgggtgac gtcgaggtca gccccgatcc
     7381 gaacggtccg gcggccctct tcgtcttcaa gacgatctcc gatcagttct ccggtcgcat
     7441 aaacctcgcc aaggtgctct cgggccacat cgcgagcgac tcccatctgg tgaacccgcg
     7501 caccggcgac aaggagcgca cgggcaacat cctcaccatg caggggaagg agagcaaagg
     7561 catcgaggaa gcttccgcgg gtgacatcat cgccctcggg aagctgaagg acacgtctac
     7621 aggcgacact ctcgccgacg cctcgttggc cgtgcgactc ccttcattcg agttcccacc
     7681 cccggccatc tccttcgcga tcacggcgaa gacgaagggc gaagaagaga aggtggcgaa
     7741 cgccctcaag cgtctcggcg acgaggaccc ggctatgcag ttccgtttcg acgctcagac
     7801 gcacgagatg atcatcgcgg ggaccagcca gatgcatgtg gaggtccttc ttgacaagat
     7861 gaagcgacgc ttcggggcgg aggtcgacct ccatccgccg agcgttccct accgagagac
     7921 catccgtaag acctcgaccg ctcagggcag gcacaagaag cagacgggcg gcagaggtca
     7981 gttcggagac tgctggatcg agatccaacc cctgcctcgt ggaggagggt tcgaattcga
     8041 agatgcgatc gtgggtggct ccatcccccg cggattcata cccgccgtgg agaagggcgt
     8101 catcgagacc atggaccgcg gcgtgatcgc agggtactcc gtcgtcgacg tgaaggtgaa
     8161 gctcttcgac ggctcctatc acacggtgga ctcgtcggag atggcattca agatcgccgg
     8221 cagtctggcg ttcgcgaagg ccatggaagg tgccgccccc gtccttctcg agcccgtcat
     8281 gaacgtggag gtcatagtcc cggaggccaa catgggcgac atcatgggcg acctctcaag
     8341 tcgccgtgga cgtccccaag ggagcgagag catgggggag atgcacgcca tccgtgccca
     8401 ggtgccgctg gccgagatgt tgacgtacgc tccgcaactc aggtcgatga caggcggtcg
     8461 cggttcctac accatggaac tcgaccacta cgacgaggtt cccgcccacc tcactgagaa
     8521 gatcgtcgag gacgccaagg ccgccagagg ggagtaggct cctctctggc gcgctctgtt
     8581 cgactacgac ggcgccgggc agcgctcagg ctgcccggcg cttagccact ttcgctcaga
     8641 ggctcctgcc gtgcgcgtct gttgggaggc aggcgtattc cccttgcccg ctgggaagga
     8701 ctcatgcaat ggttgttgta ttatctaggg catgcaatcc aagggcaccc ataggaacaa
     8761 gttggacgat ccgaagccaa aacgggtcac cgaccttctg caggaagggt tcgattcatt
     8821 ttcgcgctcg cagaaggcca tagcccgcta catcatcgac catctggagg agaccgccta
     8881 cctcagcgcg gaggagctcg cacggaaggg caacacctcc agctccaccg tcgtcaggtt
     8941 cgcccagtcc cttggattca gcggcttccc cgacctacag aaggcgacgc gagacgaaca
     9001 tcgcctcggt gcggcttcac ggcccgtggt ccacgacgac cagttgctct tcctcgtgga
     9061 agaagacaat ctcacgcggg tcctgcgaac cgatatcctg agcctcgagg agacactcgc
     9121 gaagaacacg ctgggacgct tcaaccaggc ggcggagatg atctcgacct ctcgtcagtt
     9181 gatgttggtg gggatgaacg aggcttcgat cgttgttcgg cacgcggcgt atgtgttcga
     9241 tctcctgggc ttcaccaccc acctcgtggt cgactcttcg gaagcgaacg tcgctaaact
     9301 cgttcgtctc ggccctgatg acgccgttct ggctgtgggt ttccggcgtg cgcacccctt
     9361 gaccatctcg tttcttgaga aggccgtcac ggccggcgtg cgcacggtct ccatcaccga
     9421 caattccctc tcggagatcg ccgggaaggc ctcggtcacc ctgtatgcgg atatcgactc
     9481 caccttcttc gcgcactccc tcgtaggacc cctgagcctc gtgacagctc tggcctcggc
     9541 cacctacgcc agggaccggg acgcgtgcga tgcgcgcatc aagctcgtac gggagcacgt
     9601 gggcgaatcg ggctggctcc gctagaaggc tgctgaagaa tgacgcttcg ccttgggggc
     9661 gcttgcgctg agtgttagac aaggacgtag gaagcgcgtg taccagcctc ctagccgctg
     9721 gacccccggc gttgatggcg tccgaggggt cggataaact ctagtctggc gcccacccgg
     9781 accacggggt catagcggcg tttccatgtt ggtcgaacag gcacgcggga ccgccagagc
     9841 cctggcctgt ggaagagata gcggaggtag tcatgtcggg gcactcgaag tgggcgggca
     9901 tcaagcacaa gaaggcgctt gttgacgcta aacgtggcaa ggagttcggc aagttctccc
     9961 gtgccatcac cgtggcggcg agagagggcg ggggagaccc cgaacacaac gcggcgctgg
    10021 ccacggcgat ccagaaggcg aaagacgcca acatgcccaa cgacaacatc gaccgtgctg
    10081 tcaagaaggg cacgggcgag tcggccgatg ccgccgcata tatgcatctc acgtacgagg
    10141 gatatggccc gaacggcgtg gccgtgtacg tgatggtgct caccgacaac aagaaccggt
    10201 cggcggccga tgtgcgccac atcttcgacc ggtccggggg caaccttggc accgacggct
    10261 ccgtgagctg gatgttcgag cgcaaaggag tggtctacgt cgacgccgcc gggcgcgacg
    10321 aagacgaggt catgatggtg gccatcgagg cgggggcgga ggacatgatc gttgacggag
    10381 actcgctcga gatccgcacc gagcctttcg acttcatggc tgtgaggcac gcgctcgagg
    10441 cggccgggat ccctgtggcc tccgcacaac tcaccatgat ccccaagaac acggtggcgc
    10501 tcgacgaggc cgacgcgaag aagacgttcc ggcttctcga cgcgctcgag gacaacgacg
    10561 acgtgcagga agtcttcgcg aacttcgaca tcacagatga agtcatggag gcgctcgccg
    10621 gctagggccc ctttgggacc ccggccgagc gagcgttttc gatgcccgac acaggacgga
    10681 tcatcctcgg ggtagacccg ggaaccgcct tcaccggcta cggcgttgtc tcgcagaatg
    10741 gcaaccgcct ggagacggta gccttcggtg tggtccgaac ttcaaaggac ctgcctcttc
    10801 acgagcggct cgacctgatc ttcgagggca tcggagacct ggtccgcaac taccgtccga
    10861 ctgaagccgc agtcgaatcc ctcttcttca acgtcaacgt gcgcactgcg ctggctgtgg
    10921 ggcaggcccg cggggtggcc cttctcgcct gcacgcgcgg gggctgcgac gtctttgagt
    10981 acacccccca ggaggtcaag caggccgtgg tcggctacgg caaggccgag aagagccagg
    11041 tgcaggagat ggtgcgggtg cttctcaaga tgaacgacat ccccaaaccc gaccacgccg
    11101 ccgacgcact ggcgatcgcc atctgccacg cacacggccg cgggctgcgc gccctggcag
    11161 acagagagtc cgccggtcgc cggcggccgc tcggagcggg aggcgcggca tgatagacag
    11221 gctgacaggg cgcgtggccg cgttggaggc cgacgcggct gtcctcgagg tctcgggggt
    11281 gggtttccgg gtcgagatgt cgcgcacgag tctccgcgcc ctcccggaag agggcaacgc
    11341 ggcctcgctc ttcacccacc tacacgtgag agaggagtcc ctgcagctct tcggtttctc
    11401 cacggaggag gaacgccgcc tgttcctgca cctgatctca gtaagcaaga tcgggccgcg
    11461 cctggcactt gcggctttat caatccgcaa gcctcgtgat cttcggaggg ccatcgcggc
    11521 cggcgacgta gccctcttcc agtcggtccc gggcatcggc aagaagacgg ccgagcgtct
    11581 catcctcgaa ttgcgcgaga agatggccga gatcgaaggt cccggaaccc tcatcggacg
    11641 cggatcgccc gatgaagcgg gcaacctggc cgtggcgcgc gccgccctgg cggagctcgg
    11701 catcggcgcg gccgaatcgg aggcgctgat gcgcgacacg gaccccgaca ccccggtcga
    11761 cgagatcgtg cgccaagccc ttaggcggcg gtagctatgg accacttcac accggccgag
    11821 cacggcgccg agccgggcga gcgcgacgac cgtctggccg accccgagga actcgtagac
    11881 gatcgtgagc tggaaggctc tctccgcccg cgctcgctgg ccgggttcat cggccagccc
    11941 gtgttgaggg aacagctctc catcttcatc gaggcggcga agagccgggg ggaaccgctg
    12001 gaccatgtgt tgctggccgg ccctcccggg ctcggcaaga ccaccttggc cgcgatcatc
    12061 gcccttgaga tgggggtgca catcgtcacg acgtcgggcc cggccctgga gaagaaggcc
    12121 gatatggccg ccatcctcac caacctcgaa gagggcgacg tactcttcat cgatgagatc
    12181 caccgcctga accgggttat cgaggagatc ctctaccccg cgatggagga cttcgagatc
    12241 gacatcatca tcggacaggg gcccagcgcc cggagtatcc ggctcgacct gccgcacttc
    12301 acccttatcg gagcgaccac ccgctcgggc cttatcacga cacccctgcg cgaccgtttc
    12361 ggcttctctc accgcctcga ctactactcc gaggccgact tggcctcgat catcgcgcgg
    12421 tccgcggaca tcctctgcgt ggggatagac cccgaggcca ctcacgagtt ggcggttcgc
    12481 tcccgaggca cgccacgtat cgccaaccgc ctcctcaagc gggtcaggga cttcgcccag
    12541 gtgcggcacg cggggcacgt gtcggccgag gtggggaagg aggccctccg gctgttccag
    12601 gtggacgacg aaggactcga ccgggtggac cgcgagatcc tgaacctcat cctacggaag
    12661 ttcgacggcg ggcccgtggg actatctacg cttgccgtcg ccatcggtga ggaagcggac
    12721 accatcgaag acgtctatga gccatacctg ctgcagcggg gcttcttgat gcgcactccg
    12781 cgcggcagag tgctcacaag attgggctac ctccactgcg gagcgcaacc tccagacggc
    12841 ggaccgccca cactcttcgg cggatgagac tcctgctcca catgtgttgc ggcccgtgcg
    12901 cgacggccac cctcgaccac tgggcgggtg acggggcaga cgtcacggga ttcttccaca
    12961 accccaacat ccacccgctg ctcgaattcc gcaggcgggt caccggtgtt cgcgacctgg
    13021 cgcttgcgcg acgcgtgcat gtcatcgaag acctcgccta tgatccctcc gcgtggttca
    13081 gggagatcgt gggtgaggac gcttcgggca ggtgcggcag atgtatcgcc tctcgcctgc
    13141 gtgcgaccgc ccgtgtggcg gcggcctcgg gctacgatgc cttctcgacc acgctcgcca
    13201 tcagcccctg gcaagaccac gaggccatcc gagagggtgg aggctcggca cagcaggaac
    13261 accacgtcga gttcatctat cgcgatctgc gtgcggccta ccccgactcg cgtcgagccg
    13321 cccgagaagc ggggttatac cggcagaagt actgcggttg tctcctctct gagtgggagc
    13381 gataccgtga gaaatgaagc gggcgcgacg gcgcggggcc actagccggt gcgactcgac
    13441 gagctcgact tcgacttccg cgaagacctg atcgcccaac acccgagcga gccgcgcgac
    13501 gcgtgcaggc tgctcgttgc ggatcccagg accgggagca tctcgcacca tgacttcaag
    13561 cacatcgtcg cccgcgcgcg cccgggggac gtcctcgtgg tcaacgagtc gaaggtcttc
    13621 ccggcgagga catgggcgcg caaggagacg ggcggcagag tggaactgct cttcctgcgt
    13681 cgcctcccgg acccggttcc gacagacgga gcctcggacc cggctccccc agccggctcc
    13741 tcggacgcgg acggggctga cgacaccgcc gagggtggtc ttccgacgga gacgtgggag
    13801 gtcctagccc ggccatcggc ccgactccgt cccggccgga atctcacact cgacactggg
    13861 gacaccttgg agctcgcggc tcttctgggt gacggacgct gggccctcaa gcgccgcgga
    13921 aaccccggcg tcctcgaact gttggaacta tgcgggacca tgccgcttcc gccgtacatc
    13981 cacgagccgc tccgccgggc cggtgactat cagacagtgt acgcggcggc gactgggtcg
    14041 gccgccgcgc ccacggcggg cctgcatttc acgaccgagg tccttgggac cctacaagcc
    14101 gcgggagttg acgtcgaacg cgtcaccctc cacgtgggcg tcgatacgtt tcgtccgctc
    14161 accgagttgc gcatcgaaga ccatgcgata cacagcgagt ggtatcgcgt ggagccggac
    14221 gctgttgcgc ggatagaccg ggcccgtgct gccgggcgaa gggtgatcgc ggtgggcacc
    14281 acagccgtgc gcgtcctcga gaccctctac gagggatcgg cacccggcgg gccttcctcg
    14341 ggggcccttg agggatccac ccgcgtgtac atcactcccg gctaccgttt ccgcgcggtt
    14401 gatgctctga tcacgaactt ccatctgccg cggacgtcgc tcctcgcgct cgtcatggct
    14461 ttcgggggag tcgagttcgt gcgcgaatgc tacgagactg ccgtgcgcga ggggtatcgg
    14521 ttcttcagct tcggtgacgc catgttcctc gaagcccggg cgggggaggc gtgaccgtgg
    14581 gaccgtcgct cccgaccttc cccttcgagc tcacccgcac cgacggggcg gcgcgtgccg
    14641 ggatcctcag caccccgagg ggggaagtgc cgacgccgtg cttcatgccc gtggggacga
    14701 aagggacggt gaaggccatc tcgccccacc gactgcgcga gatcggggcg cgtatcgtgc
    14761 tcgccaacac ctaccacctg gcgcttcgcc ccggaagcga taccgtggcc gagctgggcg
    14821 ggctccacgc gttcatgggt tggacgggcc cgatactcac cgacagcggc ggattccagg
    14881 tgttcagcct caaggacacc gcgcgggtgg acgacagtgg ggtcaccttc cgctccgtat
    14941 acgacggctc actgcacacg ttcacgcccg agcgggccat gagcgaacag aaccggctcg
    15001 gtgccgacat ggtcatgtgt ttcgacgagt gcgcggccgc cggcgcagat ctcgcggcgc
    15061 tcgaaggtgc ggtcaccagg accaccgagt gggcgcgccg atgctgcgat gctcacgcgg
    15121 aggtcggcgg cgtcggcacc gacggcccgc agatgctgtt cgggatcgtc cagggcggca
    15181 tagacccggg actgcgccgg agaagcgcgg agggcttgct ggaactgggg tttccggcgt
    15241 atgcggtagg cggcttgtcg gtgggggagg accgtggggc cacgctggag accaccgctc
    15301 tcaccgcaca gctactccct gccgaacgtc ctcgatactt catgggcata ggcgatccgg
    15361 tgggcttgct ggaggttatc gaccgcggca tcgacatgtt cgactgtgtg ctgcccacgc
    15421 gcatagcgcg catgggcacc gcgttcacat cgcacgggcg cgtcaacctg cggaacgccc
    15481 agcacgctca gtcgcggggg ccgctggacc cggactgccc ctgccgcgcg tgctctcagt
    15541 tcccgctggg cgccctgcgg cacttcgtca tgcagaagga gatactgggc ctgcagttgc
    15601 tcaccgaaca caatctgctc ttcttgttcc gcttggtggc gggtgccagg aacgccatcc
    15661 tcgagggaag gtacggagcg ttcaagaagg gagtgctgga gtcattcgtc cctgctgcgc
    15721 cggacagccg ctgacggcgt catggtacta tacgtggcgg tggcgccacg cctcccacgt
    15781 cacacgaacc ccaaggagga caggttgggc agcttcggtc ttctcgcact ctacgtcgtc
    15841 ggcttcatag ccatcttcta cttcctggcc atcaggcctc agcagaggca acgtaaggct
    15901 cacgatgcgc tggtgtcgtc tgtcaaacgc ggcgatcacg tcatctccgt gggcggcctc
    15961 cacggcaagg tcaagcgcac cgaggacacc gtcgtggtca tcgaggtggc caagggcgtc
    16021 gacatccgac tctcgcgcaa ggcgctcgcg gaagtcgtca gccaggcagc acaagaacag
    16081 gctgctcagc agatcgaggc cgctgagccg gaggagtccg aggaggcctg agtccggtct
    16141 cgcacggagg tccacgccgg cggcgactga ttgacatccc cgccacagtg caatatcctg
    16201 cgcctccgcg gtccggcccc ttactcctgc ctatccgcac gtacacaatc tgacgatccg
    16261 aggaaggtgc gaccttgtcc gcgatgcagc gcaacgttgt gatcttggcg gttgttgcgg
    16321 tcctgctctg cgtggctgcg tacttcatct acccacccgg aagcagcacc cgactcggtc
    16381 tggacctgca aggcggcctc gcagtcatct tggaagctca ggattccgcg cgggccccgc
    16441 gcaccgacga cggcatgaag cagaccatca gcatcatcaa cgaccgcatc aaccgactcg
    16501 gtgtcaccga accggaagtg cagcggcagg gcgagtggaa gatcagtgtc cagctgcccg
    16561 gcatcgagaa caagcaagag gctctcgcca tcatcggcaa gaccgccgtc ctcgagttct
    16621 acgacgtgaa agacttcgga gcggcgtacg cctccgaggc ggaagccctg aaggctgcgg
    16681 gtgtcgactc cgaggcgcag ctccccgccg acacacggct ggttcgccgg ccttcccgcg
    16741 acggcacgct ccccgaccgc ttctacatcg tcacggccat cccagcggtg accgggacag
    16801 gtctctcggg tgcgtcggtg ggcttcgaca acaacaacca gccgaagatc gacatgcagt
    16861 tcaagtcgga cggcgcgcag gcgttcgctg aggtcaccca ggctctggcg gacagggcac
    16921 aggtcaccgg ccaggaccag ctgctggcga tcgtcctcga tggagtcgtg cagtcggctc
    16981 cgcgggtggc ggagcgcata gatggcggtc gcgccgagat caccggtcgc ttcaccctcg
    17041 acgaagccaa gaacctcgtg ctggtcctgc agacgggcgc actgcccatc gaactgaagg
    17101 tcatcgacga gcgctcgatc ggtgctacct tgggcaaggc ctcactcact caggcccttt
    17161 gggcgggtgt cgtcgggctg atcctggtgg ctgtattcat ggtcgcctac taccgactac
    17221 tgggactcgt ggcggatctc actctcctga tctatggcgt gctcttctgg gggatcctca
    17281 attccatcca tgcgacgctc actctcccgg gcatcgcagg catgatcctg accttgggga
    17341 tggcggtcga cgccaacgtg atcatcttcg cccgtatccg tgaagaggta cacgccggga
    17401 agacggtgcg caccgcggtc gaggcgggct tcaggaaggc attgagcgcg atcctcgacg
    17461 cgaacgtgac caccttgatc actgcggtgg tgcttttctg ggctgccagc ggaggagtac
    17521 gcgggttcgc cctcaccctg gggatcgggg tagccctctc catgttcacg gcgatcgtcg
    17581 tgaccagagc actgctcgcc ctgctctcgg gcctgaagat cttcaagaac cccacgctca
    17641 ctgggctgag cgtcgaaacg gagcgctaga tgaccgtcta tcccttcatg aaatacaagc
    17701 ggtggtcggt gatcttgctg ggcgcggtga ccctctttgc cgcgggtgcc atcctggtgc
    17761 tcggacttcg gctgggcatc gaattcaacg gtggggtccg cctcgaggtg caactcaagc
    17821 aggatgcggc gacatctgac gttcgcacca tcgtgtccga ggccggtatc gccgacaccg
    17881 tggtccaacc cgtgggaagc aagggcttcg tcatcaccgc ggcagagatg tcggatgcgc
    17941 aggtcgcctc cgtgatcaaa gcgctcggcg acacgtacgg agcggaggag tcggcgacag
    18001 gtcaagagcg cgtgggcgcc agcttcggcg ccgagacggg acagaaggcg ctcatagcca
    18061 tcttggtggc ggtcgccgtg atcatcctgt acatcacctg gcggttcgag tacaagttcg
    18121 cgtttccggc catcgtggcg ctggtgcacg acgtggcctt gacccttggc gtgtacgccg
    18181 tgaccggccg cctggtcacg acggcgaccg tcgcagccgt gctcactatc gtcgggtact
    18241 cggtgaacga cacgatcatc gtcttcgacc ggatcaggga gaatcaagcc ttcatgaaga
    18301 aggagtccta cggctcgatg gtcgacctat cgatcagaca gaccctggcg cgctcgttga
    18361 acacggcgtt gaccaccctg ctgcccatag ccacgatcct cttgttcggc ggctcgacgc
    18421 tgaaggactt cgccttcgca ctcttcatcg gtatcgcctc tggcgcctac tcgtcgatct
    18481 tcgtggcttc gccccttctc gcctactgga aggaacggga gcctcggtac cgtcgccgag
    18541 cgatggccga caagaccgcc tgaggctcct ttggtagcct ggaggcgacc gcgccttccg
    18601 gggagtcaag cgcccgttgt tttgcggtcg ccagtggccg ggaagagtcc ccacggacat
    18661 tcttcgtctg acgggagggt tcatgaccga acagcatgac gaccggcgtt ccgaggatca
    18721 ggctgtggag gctaccggtt tacgggatat catcgaacgg accctgctcg tgggtctggg
    18781 cgccgccagc ctcacgaagg aacgtctcca gaaagtcgcg gacgagttcg tgcggcgcgg
    18841 ccaactctcg gccgaggacg gccgcgccct ggtggaggga ctcgcggccc gtagtcgcga
    18901 ggaagcccgc tcggccctca agagcgccga cagctccgtg cagaccgtgt tcagggaact
    18961 ggggatagcg tcgcgacgcg agctcgaaga actcgacttc aagctccgcc agatcgagca
    19021 tcgtctcgcc ctgctggaga gggagtccga ctcttcgtct gccgcctcga ccgactgagc
    19081 ccagtgccat tgtgacgacg aggactgcac caagccgacg actccgatga cccagcgcta
    19141 ccagggcctc cgcgggcgcg ctcgaaacct cgaacgcctc aggcagatat ccgaggtggc
    19201 ggtccggcac ggcttgggct acttcttcga gcgccataac ttgtggcaca tcctgtccgt
    19261 gagacgccgc gaggtccagc cgccgccgtc tcagctgggc cgccacatcc gggagttcct
    19321 ggaggagctc ggccccagct tcgtcaagtt cgggcaacta ctcagcacca ggtccgacct
    19381 gctccctcac gagatcgtgt cggaactcgt caagctccag gacagtgtgc ccgccttccc
    19441 ggtggaggaa gccagggccg tcttgagaga ggatctcggg ctcaccgcgg agcgcctgtt
    19501 cgagtctttc gaacccgtgc ccatcgcggc cgcctcgatc ggccaggtgc accgcgccgt
    19561 actgcccgga ggcatgcagg tcgtggttaa ggtgcagcgg ccgagggcta tggggcagat
    19621 cgagcgagat atcgacctgc tgtaccagat cgcggagctc ctctccgagc acttcggaga
    19681 gcgactcgtg gtggatcccg tgaaagtggt cgatgagttc gcccatggga tctcggcgga
    19741 gctcgactac accctcgagg ggcgcaacgc cgagaggttc gctgccaatt tcagcgcgga
    19801 gccagccgtc gccatcccga gggtcttctg gac
//
LOCUS       DASRMJ010000043         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_39371, whole genome shotgun sequence.
ACCESSION   DASRMJ010000043 DASRMJ010000000
VERSION     DASRMJ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39371"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /gene="tsaE"
                     /locus_tag="VFD74_01340"
     CDS             complement(<1..477)
                     /gene="tsaE"
                     /locus_tag="VFD74_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZK48230.1"
                     /translation="MVVQIECIDLKGLGELAQRFAAALREGDLVLLEGPLGAGKTTFV
                     RMLCHELGVTDQVRSPSFTIANVYEGPVPVNHLDLYRLEGTDDEDVLALEDYVGPSVI
                     TMVEWPDAGRGLLGRPTWVVALEHRDLETRGLRIEAMVPDTAATLADVSNARTRDRA"
     gene            complement(528..1490)
                     /locus_tag="VFD74_01345"
     CDS             complement(528..1490)
                     /locus_tag="VFD74_01345"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZK48231.1"
                     /translation="MTHTASPSGDRDEAAAEPRDPAIGAAPEADTAEPPDPASGAVPR
                     PGAAELRGVAAAELSDQAARLADCRECRLSEGRTQVVFGVGNPRAELMFVGEGPGFHE
                     DRLGEPFVGQAGKLLTELLASIGLRREDVYIANVVKCRPPENRDPLPDEIEACSPNLV
                     RQIQTIRPRVICTLGRLATRLVAETEAGISSIRGKAKPMRVAGVPVVVLPVFHPAAAL
                     YTPANRAALEEDFLKLRRLLSAGEKALIGNEATAPAASARAGASGAVERPPESVELDE
                     SSSREMSPAEALPAPATASDRRGRPAPPPGDAAKAGPSEQLPLW"
     gene            complement(1568..1777)
                     /locus_tag="VFD74_01350"
     CDS             complement(1568..1777)
                     /locus_tag="VFD74_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZK48232.1"
                     /translation="MGKRLARSRDEKMVCGVAAGLARYFDLDPTIMRLIWVVAVLLPG
                     PNLIIVAVYLVLCLLMPLETPGGTA"
     gene            2172..3668
                     /locus_tag="VFD74_01355"
     CDS             2172..3668
                     /locus_tag="VFD74_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZK48233.1"
                     /translation="MSFALALRSAGATVVGHTCRTPGGRSAAEAVLGTPSSPSIPDVV
                     AAAFAPDRAVRSGSQPPDELIVFLLTVPDRELPTVASALAEAVSRHTGAASSWAGSSR
                     ADSSSPAPSWAAVHTSGATSVTVLSPCAATGALTLSLHPLQTFSDPLRGAAALRGAAM
                     AVTPGPDPDRAAEARSVGERLARSVGGRPFLLDEDHRSLYHAAATVASNYLVTLEHVA
                     EHLFQLAGIPEEEALPALLPLVRGAVDNIEAQGTVRSLTGPLSRGDLETLAAHVSALL
                     AKAPESVDLYRVLGTATLGVVRRRGELPDAVIHSMEKIMSGIAESETQPTAAAPATAT
                     PESAAPTAAAGAGRPPSDQVSGGLTVATRSIAFSTQGEGDVIDLTDQLNRLLRETGLS
                     AGTLTVFAPGATGAVTTLEFEPGVVHDFKQLFGRVIPAQGTYRHNLLLSDGNGHSHVR
                     AGLLGPSLVIPFVEGRLTLGTWQEAVFVCFDTRPRDRKIVVQIMGVTQ"
     gene            3668..4972
                     /gene="hxlA"
                     /locus_tag="VFD74_01360"
     CDS             3668..4972
                     /gene="hxlA"
                     /locus_tag="VFD74_01360"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019647 - formaldehyde assimilation via
                     ribulose monophosphate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HZK48234.1"
                     /translation="MSEKNSQGPIVQIALDFVDLPRALRVAEEAVRGGADWLEAGTPL
                     IKSEGLEAVRELKRLWPDKTIVADMKTMDAGRAEVEYAAKAGADVVGILGAASDATIR
                     ECVEAGRNYGARIIVDMVQVADIVERARAVEAMGASYIGIHVAIDEQMEGKTPFDVLR
                     RVATAVSIPVAVAGGINSETAADAVKAGASIVVVGGAVTKATDAAEATRQIKGAIERL
                     ESVKSEYFVRATGPDIRGVLSRVSAANVSDALHRTGDLPGLLARTPGAKMVGPALTVR
                     TYPGDWAKPVEAIDEAAEGDVIVIDAGGVPPAVWGELATNSAIQRKLAGVVIHGAARD
                     TGDIRALGFPLFTSHVTPTAGEPKGFGEIGVPIRIAGVSIAPGDWIIGDDDGVCVIPR
                     EKAVEYTNRAMDVLEKENRLREEIRQGRTLSEVAYLLKWEKR"
BASE COUNT          835 a         1840 c         1704 g          794 t
ORIGIN      
        1 ggcccggtcc ctcgtcctgg cgttcgatac gtcggcgagc gtcgctgccg tatcggggac
       61 catcgcctct atccgcaatc cgcgggtctc gaggtctcga tgctcgaggg cgacgaccca
      121 ggtcggtcgc ccgagcagac cgcgcccggc gtccggccac tccaccatgg tgatgacgct
      181 cggcccgacg tagtcctcca gtgccagcac gtcctcgtcg tccgtgccct ccagtcggta
      241 gaggtcgaga tggttcacgg ggacgggacc ttcgtagacg ttcgcgatcg tgaacgaggg
      301 gctgcgcacc tggtcggtca ctcccagctc atggcagagc attcggacga aggtggtctt
      361 gccggcaccc aatggacctt cgaggagcac gaggtcgcct tcgcgcagcg cggctgcgaa
      421 gcgctgtgcc aactccccga gtcccttcag gtcgatgcac tcgatctgaa ccaccacgag
      481 gtctccgacg acaccgctct gttctgcccc gtccaccgcc ggcggcccta ccacaggggc
      541 agctgctcgg acggaccggc cttggcggca tcgcccggcg gcggagcagg ccggccgcga
      601 cgatccgagg cagtcgcggg cgctggcaag gcctccgcgg gcgacatctc gcgtgatgac
      661 gattcgtcga gttcgacgga ctcgggcggt ctctcgacgg cgccggaggc gccggcccgg
      721 gcagacgcag ccggcgccgt cgcctcgttc ccgatcaagg ccttctctcc cgccgacaag
      781 agacgccgca gcttcaggaa gtcctcttcc aaggcagcgc ggttcgcggg cgtgtagaga
      841 gcggcggcag gatggaacac cggcagcact acgaccggga cgcccgccac gcgcatcggc
      901 ttggccttgc ctctgatcga gctgatcccg gcctcggtct cggcgacgag gcgcgtggca
      961 agacgaccga gagtgcatat gacccgggga cggatcgttt ggatctgccg aaccagattg
     1021 ggggagcacg cctcgatctc gtcgggcaac gggtcgcggt tctcgggggg acggcacttc
     1081 accacgttgg cgatgtacac gtcctcgcgc cgcagcccga tggaggcgag caactcggtg
     1141 agcagcttgc cggcctgtcc gacgaatggc tcgcccaggc ggtcctcgtg gaagccgggg
     1201 ccctccccaa cgaacatgag ttcggcacgg gggttcccca ccccgaatac gacctgtgtg
     1261 cgaccttcgc tcaggcgaca ttcgcgacaa tcggcgagcc gggctgcctg gtcgctcagt
     1321 tctgcggccg cgaccccgcg aagttccgct gcgcccggcc gaggtacggc gccggaggcg
     1381 gggtcgggcg gctccgcggt gtccgcttcc ggtgcggcgc cgatggctgg gtcgcgcggc
     1441 tccgcggcag cctcgtcgcg gtcaccgctg ggtgaggcag tgtgggtcat aggagtgggt
     1501 cctccggcac ttccatccct tcaggacggc cgcctctcgg atcaccacac cccggggggc
     1561 gcaaaggtca cgcggtcccg cccggagtct cgagaggcat gagcaagcag agtaccagat
     1621 agacagccac gatgatcagg ttggggcctg gcaggaggac ggccaccacc cagatgagcc
     1681 gcatgatcgt cgggtcgagg tcgaagtagc gggccaagcc cgcggcgacc ccgcacacca
     1741 tcttctcgtc acgcgaacgc gccagtctct tgcccattgg gactccttcc gggcccggtg
     1801 ccgccgggcg ccttagtctt ccattggttt ttcatctacc cacactacgg gggagagcac
     1861 gcagttgttc caggcacccg cccgctgcta tccttattgg cagcgcgcag tcgcgggacg
     1921 tccacttcgt ggggtgtacc ggcggccgac tggtcctcgg gcaatgtcgg cgacgcgtcg
     1981 agccgatccg tatcgacctg agtctggtat ctgcacacag aactggaacg agaggtcaca
     2041 tcatcacaac gcacccgcgt cacccgcgga gcactccgca cctaggtcaa ccatcggtgg
     2101 acaccggctc tcccccggac ggtcgcgccc gtgaccctcg ctacgccgtc gtcggagctg
     2161 gacgtctcgg gatgtcgttc gccctcgccc tccgcagcgc gggagctacc gtagtgggac
     2221 acacctgccg aacacccggc ggccgctccg cggccgaagc cgtcctgggg acaccgtcgt
     2281 cgcccagcat acccgacgtc gtggccgccg ccttcgcgcc ggaccgggcg gtgcgctccg
     2341 gctcccagcc gccggacgaa ctcatcgtgt tcttgctcac cgtgcccgac cgcgagttgc
     2401 cgaccgtcgc ctccgcactc gccgaggcgg tctcgcgtca caccggggca gcctcctcct
     2461 gggcgggttc ctcccgggcg gactcctcct cgccggcccc ctcctgggcc gccgtccaca
     2521 ccagcggagc tacctccgtg accgtactgt ccccgtgtgc ggcgacggga gccttgaccc
     2581 tctctctcca ccccctgcag accttcagcg acccgttacg aggcgccgct gcgctccgcg
     2641 gagccgccat ggccgtcacg cccggtcccg atccggatcg cgccgccgaa gcccgcagtg
     2701 tcggcgagcg gctcgcccgg agcgtcggcg gccggccctt cctgcttgat gaagaccacc
     2761 gcagcctcta ccacgccgcg gccacggtgg cgagcaacta cctcgtcacc ctggagcacg
     2821 tcgccgagca tctcttccag cttgccggga tacctgaaga ggaggccctt ccagctctgc
     2881 tgcccctggt gcgtggcgcc gttgacaaca tcgaagctca ggggaccgtc cgcagcctga
     2941 ccggacccct gagccgcggc gacctcgaga cgctcgcggc acacgtgtcc gcactcttgg
     3001 cgaaagcccc ggagtcggtg gatctgtacc gagtactggg tacagctacg cttggagtcg
     3061 tccgtaggcg cggtgaactt cccgacgccg tcatccattc gatggagaag atcatgagcg
     3121 gcatcgcaga atcagaaacc caaccgaccg cagccgcccc ggccaccgcc acccctgaat
     3181 cggccgcacc gacagccgcc gccggcgccg ggcgaccccc gtccgaccag gtcagcgggg
     3241 gcctcaccgt cgccacccga tccatcgcct tctccacgca gggcgagggc gacgtcatcg
     3301 acctgaccga ccaactcaac cgcctgcttc gcgaaaccgg cctctccgcg ggtacgctca
     3361 ccgtcttcgc gccgggagcc accggagcgg tcaccacgct ggagttcgag cccggcgtcg
     3421 tccacgactt caagcagctg ttcgggagag tcatccccgc acaaggcacc taccggcaca
     3481 acctgttgct cagcgacggc aacgggcact cccacgtccg ggccggtctg ctcgggccct
     3541 cattggtgat cccgttcgtc gagggacgcc tgaccctggg gacgtggcag gaagccgtct
     3601 tcgtatgctt cgacacccga ccacgagacc gcaagatcgt ggtccagatc atgggagtga
     3661 cacagtgatg agtgagaaga actcgcaggg cccgatcgtc cagatcgcct tggatttcgt
     3721 agaccttccg cgcgccctga gagtggccga agaagcggtg cgcggtggag ccgactggtt
     3781 ggaggcgggc accccactca tcaagagcga aggacttgag gcggtccgcg aactcaagcg
     3841 cctgtggccc gacaagacca tcgtcgccga catgaagacc atggacgccg gccgagccga
     3901 ggtggagtac gcggcgaagg ctggagccga cgtggtcggg atcctcggcg ccgcctccga
     3961 cgcgacgatc cgcgagtgcg tggaggcggg ccgcaactac ggcgcccgca tcatcgtgga
     4021 catggtccag gtggccgaca tcgtggagcg agcgcgcgcg gtcgaggcca tgggcgcctc
     4081 gtacatcggc atacacgtgg ccatcgacga gcagatggag ggcaagactc cgttcgacgt
     4141 gctccgccgc gtggccacgg cggtctccat accagtcgcg gtagcggggg ggatcaactc
     4201 cgagacggcc gccgacgccg tcaaggccgg agcttccatc gtggtcgtgg gtggagccgt
     4261 gaccaaggcg acggacgccg cagaggccac ccgccagatc aagggagcca tcgagcgcct
     4321 cgagtcggtc aagtccgagt acttcgtgcg cgccaccggc cccgatatcc gcggggtgct
     4381 gtcgcgtgtt tcggccgcca acgtcagcga cgcgctccat cgcacgggag atctacccgg
     4441 acttctggcc cggacacccg gggccaagat ggtgggcccc gcgcttacgg tgcgcaccta
     4501 tcccggcgac tgggccaaac cggtcgaggc catcgacgag gcagcggagg gcgacgtcat
     4561 cgtgatcgat gccgggggcg tccccccagc cgtgtggggc gagctggcca ccaactccgc
     4621 catccagcga aaactggcgg gcgtggtcat ccacggagcc gcccgcgata ccggcgacat
     4681 ccgagctctc ggcttcccct tgttcacgag ccacgtcacc cccactgccg gcgaacccaa
     4741 gggcttcggc gagatcggag tgcctatccg catcgcgggg gtgagcatcg cccctggcga
     4801 ctggatcatc ggtgacgacg acggggtctg cgtgatcccg cgggagaagg cggtggagta
     4861 cacgaaccgg gcgatggacg ttctcgagaa ggagaacaga ctacgcgaag agatccgcca
     4921 gggcaggacc ctgagcgagg tggcgtatct cctcaagtgg gagaagcgct aggccggacc
     4981 acatcgtccg gatctctcga ccgaagggcg tgaagatggc cttcttcaac tgggctgctc
     5041 cctacatgca ccgctacctc gacggccgtt ggacggcgga tacgacggcc gcgcttgctc
     5101 acgccctcgg tccctacctg tcgtcgcctt cgccggcccg gcccgggaga ctcctcgacc
     5161 tcggaggtgg cac
//
LOCUS       DASRMJ010000044         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_147666, whole genome shotgun sequence.
ACCESSION   DASRMJ010000044 DASRMJ010000000
VERSION     DASRMJ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147666"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /locus_tag="VFD74_01365"
     CDS             <1..1617
                     /locus_tag="VFD74_01365"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HZK48235.1"
                     /translation="LLDIPLGAVALTKIDMVDEEMVELAAADVAEFLATSRYAEAPII
                     PVSGVTGAGLDDLRLALDGLAARAPRRGAYPATRMPIDRVFSLKGIGTVVTGTLWSGE
                     LSAEETVAILPPVGAVVLDDVRVRSVQVHDHAVEAAEEGQRVALNLTGVDRQQLQRGQ
                     WVIKDPVIEPSYLADVSLVLLDDATEALTRVSRVRVDHGTQEVIAKVVLADREELLPG
                     ETCYAQIRFEDRVVVYPGDHFIMRALTPVTTIGGGRVFDPDPRKHGTDPKWRERLSVL
                     EEGPAESIVELLLTEAFPKGILRRHLESSPYLWRFDAPPAVATLLRDGRALEGPQQKL
                     FHGPSLETLERTVVDTLRLRAEADVLNPYLSVGELRRAVSEGKDWPALDAALERLQSG
                     GEVVRTEHGLRWGQAEGALEGHDADRAEGLLALYERIGLEAPSVAAAALEAGLRDSDA
                     LRLVQALARQGRMAKVGEDLYYATSTLDEVMAKIAAEMESRGQITLAEVRDLFATSRR
                     YAQALLEHMDSEGMTLRIGDARRLRKRRRS"
     gene            1740..2666
                     /locus_tag="VFD74_01370"
     CDS             1740..2666
                     /locus_tag="VFD74_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZK48236.1"
                     /translation="MSSTVVNWGDMPTRVVDRGPFGVRWTNLGEAIGTRGTGARRMEI
                     EPGRRSTPAHAHTAEEEIFYVLGGSGLSWQDGVTFAVSAGDCLVHLPLAEAHTLVAGD
                     DGLDALVFSNRRPVEACRLPRAGVSWLGPTWVATGEDPNPWGREIAAGELECPLPSER
                     PARIVALADVPAREGGRGDFQRTLRPVSRAAGSRDLGLNHVHVPEGKLSYPLHCHSTE
                     EEIFVVLEGHGTFMLGSDEHELRPGSVVCRPAASGVAHAVRGGAGGVTYLAFGDRNNA
                     DMTWYPTSKKVAFSGLGVIGRLEPLEYYDGEV"
     gene            complement(2648..2800)
                     /locus_tag="VFD74_01375"
     CDS             complement(2648..2800)
                     /locus_tag="VFD74_01375"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HZK48237.1"
                     /translation="MRVGSGGDSVGRILQNYPGITEGDIRACLGYAALLTREQVLTPL
                     AHTSPS"
     gene            3118..>3306
                     /locus_tag="VFD74_01380"
     CDS             3118..>3306
                     /locus_tag="VFD74_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48238.1"
                     /translation="MNRMVVKGSLATVGIVLVIIVVMFAIVGCGGTTTTTTAVQTAIT
                     AGTTETTAAPVEAKTAVIG"
BASE COUNT          523 a         1055 c         1196 g          532 t
ORIGIN      
        1 ctcctggaca tcccgctcgg agcggtggct ctcaccaaga tcgacatggt cgacgaggag
       61 atggtggagc ttgccgccgc cgacgtggcg gagttcctgg caacctcccg ctacgccgaa
      121 gctccgatca tcccggtgag cggcgtcaca ggcgcgggtc tggacgatct gcgtctggcg
      181 ctcgacggac tggctgcccg ggccccgagg cgcggcgcct atcccgccac ccgcatgccc
      241 atcgatcggg tcttctcgtt gaagggcatc ggcaccgtgg tcacgggcac gctttggtcg
      301 ggagagctga gcgccgagga gacggtggcc atcctgccgc cggtgggggc ggtggtcctc
      361 gacgacgtgc gggtgcgcag cgtccaggtg cacgaccacg ccgtggaggc cgcggaggaa
      421 gggcagcgcg tggcactcaa cctcacgggg gtagatcgtc agcagctcca gcggggccaa
      481 tgggtgatca aggacccggt gatcgagccc agctatctgg ccgacgtgtc cctggtgctc
      541 ctcgacgacg ccacggaggc gctcacccgg gtgtcgcggg tgcgcgtgga ccacggcacc
      601 caggaggtga tcgccaaggt cgtcctggcc gatcgggagg agctccttcc cggcgagacc
      661 tgctacgcgc aaatccgttt cgaggaccgc gtcgtcgtgt atccgggcga ccatttcatc
      721 atgcgcgctc tcactcccgt gacgaccatc gggggaggca gggtcttcga ccccgatccc
      781 cgcaaacacg gcaccgaccc caagtggcgc gagcgcctca gcgtgctcga ggaggggccg
      841 gccgagtcca tcgtcgagtt gctgctgacc gaagctttcc cgaaaggcat cctccgccgc
      901 caccttgaat ccagtcccta cctctggcgc ttcgacgctc cgccggcggt ggctaccctg
      961 ctgcgcgacg ggcgtgcctt ggagggtccg cagcagaagc tgttccacgg accgagtctc
     1021 gagacgttgg agcggaccgt ggtggacacg ctgcgtctgc gggccgaggc cgatgtgctc
     1081 aacccttatc tctcggtggg cgagttgcgc cgggcggtgt cggagggcaa ggattggccc
     1141 gccctcgacg cggccctcga gcggctgcag tccggcggtg aggtggtgcg caccgaacac
     1201 gggctccgct ggggtcaggc ggagggagcg ctcgaaggtc acgacgccga ccgggcggag
     1261 ggtctgctgg ccctctacga gcgcatcggg ttggaagcgc cgtcggtggc ggccgccgcc
     1321 ctcgaggccg gactgcgcga cagtgacgcc ttgcgactgg tgcaggcact ggcacggcag
     1381 gggcgcatgg ccaaagtggg cgaggatctc tactacgcca cctccacgct cgacgaggtc
     1441 atggccaaga tagccgccga gatggagtcg cgtggccaga tcaccctcgc cgaggtgcgc
     1501 gatctgttcg ccacttcgcg gcggtatgcg caggctctgc tggagcacat ggattcggaa
     1561 gggatgaccc tgcgcatcgg cgacgcccga cgtctgcgaa agcgccgccg cagttgatag
     1621 gggcgccgca gctgtgacgg gcgccacagt tggagagcgc cgaagttgac atgggcgccg
     1681 gacctgaggg gagcgtcgtg gcccgcgcgg ggcgccgcgg ccgggaagag gaggagagga
     1741 tgagtagcac ggtggtgaac tggggcgata tgcccacccg ggtagtcgac cggggacctt
     1801 tcggcgttcg ctggaccaac ctgggtgagg cgatcggcac cagaggaacc ggtgcccgtc
     1861 gcatggagat cgaaccgggc cggcgctcca cgcccgcaca tgcgcacacg gcggaggagg
     1921 agatcttcta cgtgctgggc gggagtggac tcagttggca ggacggcgtc accttcgcgg
     1981 tgtcggccgg agactgcctg gtgcacctgc cgttggccga ggcgcacacg ctcgtggcgg
     2041 gtgacgacgg tctggacgcc ctcgtgttca gcaatcgcag gccggtagag gcctgtcgcc
     2101 tgcctcgggc cggcgtatcg tggctcggcc ccacctgggt cgccaccggc gaggacccga
     2161 atccctgggg acgggagatc gcggccggcg agttggagtg tccgctgccg agcgaacgcc
     2221 cggcgcgcat cgtcgctttg gccgacgtgc cggcgcgcga aggtgggcgc ggtgacttcc
     2281 agaggaccct gcggccggtg agtcgagcgg cgggctcccg cgacctcgga ttgaaccacg
     2341 tgcacgtgcc cgaagggaag ctctcctatc cgctgcattg ccactcgacc gaggaggaga
     2401 tcttcgtcgt gctggagggg catggcacct tcatgctcgg cagcgacgaa cacgagttga
     2461 ggccgggttc cgtcgtatgt cggcccgccg ccagcggggt ggcccacgcg gtgcgcggcg
     2521 gcgccggcgg cgtcacctac ctcgcctttg gcgaccgcaa caacgccgac atgacctggt
     2581 atcccacctc gaaaaaggtg gccttcagcg gtttgggtgt tatcgggcgg ttggagcctc
     2641 tggagtacta cgacggcgag gtgtgagcga ggggcgtcag aacctgctcg cgcgtgagga
     2701 gcgccgcgta gccgaggcag gcgcggatgt caccctcggt gatacccggg tagttctgaa
     2761 ggatgcggcc gaccgagtct ccgccggacc ctacgcgcag tagacccgca cgttgtcccc
     2821 gttcttcgag tccacgttca cttccatctc ggccagaccc tcgatgagtt cctcaacgtc
     2881 atccggcttc agatgtggga cctttcgcgg ggatcgctct gattctagtc ggagtagcgg
     2941 tgcagagcgg cgtttgatag agtctacgcg gaatcagcaa cgccgggacg gggccccacg
     3001 gcagtcagga tccccatgac gcggcatagg ttgcatgtgg gcctgcgcgt tgcccgacgc
     3061 ggttcgtgcc catccgggtg tcggatcggc ggagtcactc aacatcggag gtgccacatg
     3121 aatcgaatgg tagtcaaggg gtcgctcgcc acggttggga tcgtgctagt catcattgtc
     3181 gtgatgtttg cgatcgtcgg ctgtggcgga acgaccacaa ccacgaccgc cgtccagacg
     3241 gcgatcacgg cgggaaccac cgagaccacg gccgcgcccg tcgaggccaa gaccgcggtc
     3301 ataggc
//
LOCUS       DASRMJ010000045         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_292230, whole genome shotgun sequence.
ACCESSION   DASRMJ010000045 DASRMJ010000000
VERSION     DASRMJ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292230"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFD74_01385"
     CDS             complement(<1..608)
                     /locus_tag="VFD74_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48239.1"
                     /translation="MLLVLAATLVLAMGVGPAGADTTEFRMTGDLLLGPGQTVDTLVT
                     LNGDITVEGHVLQDAFTANGDIHVLSGGRIDGDAVSLTGQVVVDEGGVVSGDRVEIGG
                     GGGVVRIADPRPIVIDRNVGFSGPGWFFWLLGGMGLGLLLVLLAADSLKSVGNEVSTR
                     TARSALVGFLSPLGLLVLFVVLLISVIGIPVALLLIPLVPFTA"
     gene            complement(696..1262)
                     /locus_tag="VFD74_01390"
     CDS             complement(696..1262)
                     /locus_tag="VFD74_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZK48240.1"
                     /translation="MTWSDDCLSNEQLFFYSEGLLDADETRRVATHLRSCEPCAVRYR
                     AEIEFTVALRELPAPLPSPAMPSRVIQKIRAARSGRVSTWWWVVAFGLLVASGLRWLV
                     VGGFSPQKGVESLTAFLWSATIALLQLVTRFGDLETWRQVGRSFVTVAEAAGASPVWL
                     ASSVVAAVMVAVATNVLLWKVARRALAR"
     gene            complement(1265..1873)
                     /locus_tag="VFD74_01395"
     CDS             complement(1265..1873)
                     /locus_tag="VFD74_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK48241.1"
                     /translation="MEAVLTNMRVCVNRPGTTDEDLAARAQQGDLRAYEALVDRYQRF
                     VFRVLWSRAGSSREDIEDLTQDTFVRAWERLDTFDPSRPFKAWIARIAGNVAIDRHRS
                     NMRRPSTIEISDVEHELASSEPGPASAAIGDEQRRHLLSRLKELPELYREVIVLRFIE
                     DQSYEEIAEALDLPLGSVKTRIFRGRELLKQRLSPDLAEGGA"
BASE COUNT          389 a          816 c          715 g          435 t
ORIGIN      
        1 gccgtgaacg gcacgagtgg gatcaggagc aacgcgacgg ggatcccgat caccgagatg
       61 agcaacacca cgaacagcac cagcaggccg agcggagaga ggaaccccac cagcgcgctt
      121 cgcgccgtcc gggtggacac ctcgttgccc acactcttca gactgtctgc cgcaagcagc
      181 accaggagca gccccagccc catccctccg agaagccaga agaaccagcc cgggcccgag
      241 aagcccacgt tcctgtcgat gacgatgggg cgcggatcgg cgatccgcac cacgcccccg
      301 cctccgccga tctccacccg gtcgcccgag accacgccgc cttcatcgac gacgacctgg
      361 cccgtgaggc tgaccgcgtc tccgtcgatc cggcctccgg acagcacgtg gatgtcgccg
      421 ttggctgtga aggcgtcctg gagaacgtgt ccctcgacgg ttatgtcgcc gttgagggtc
      481 acgagagtgt cgacggtctg tccgggaccg agcagcaggt cgcccgtcat gcggaattcc
      541 gtggtgtccg caccggccgg ccccacgccc atcgcgagga ccagcgtcgc ggctagcacc
      601 agcagcatgc cgaggccgag cgcccgttgt cttgtcgccc gtcttgcctg ttgcaccgtt
      661 gccggcatcc gtgtcacttc tccgcctttc gtcatctatc gcgccagagc ccgcctggcc
      721 accttccaca acaacacgtt cgtcgcgacc gccaccatga cggcggccac caccgagctc
      781 gccaaccaca ccggggacgc tcccgccgcc tcggccaccg tcacgaacga gcggccgacc
      841 tgcctccagg tctccagatc gccgaagcgg gtcaccaatt ggagcagagc gatcgtggcg
      901 ctccacagga aggcagtcag cgactccact cccttctgtg gcgagaaccc acccaccacc
      961 agccagcgca gacccgaggc gacgagcagg ccgaaggcca ccacccacca ccacgtcgac
     1021 acacgcccgc ttctggctgc gcgtatcttc tggatcacgc ggctcggcat cgcgggggac
     1081 ggcagaggag ccggcagttc tcgcagagcc accgtgaact cgatctccgc gcgatagcgc
     1141 accgcgcagg gttcgcacga cctcaggtga gtcgcgactc ggcgggtctc gtccgcgtcg
     1201 agcagtcctt cggagtagaa gaagagttgt tcgttcgaaa ggcagtcgtc ggaccaagtc
     1261 atgactatgc tcctccctcg gcgaggtccg ggctgagccg ctgcttcagc agctcccgcc
     1321 ctcggaagat ccgcgtcttc acggaaccca agggcagatc gagcgcctcc gcgatctcct
     1381 cgtacgattg atcttcgatg aagcgcagca cgatcacctc gcggtagagc tcaggcagtt
     1441 ccttgaggcg tgacagcagg tggcgccgct gctcgtcgcc tatggcggcc gaggccgggc
     1501 cgggctcgga tgatgcgagc tcgtgttcca cgtcggagat ctcgatggtg gacggccggc
     1561 gcatgttgct gcggtggcgg tcgatggcca cgttgccggc gatcctggcg atccaggcct
     1621 tgaagggacg tgacggatcg aaggtgtcga gccgctccca cgcccgcacg aacgtgtcct
     1681 gggtcaggtc ttcgatgtcc tctcgcgatg agcccgcccg cgaccacagc acgcggaaca
     1741 cgaacctctg gtagcgatcc accagagcct cgtaggcgcg caggtcgcct tgctgtgccc
     1801 gcgcggccag gtcctcgtct gtcgttcctg ggcggttcac acacacccgc atgttcgtga
     1861 gcacagcttc catcgcacca acctgtcgat tgcccgtctg ctgaatgctc tacgggccct
     1921 gaggctgtga tgtttcatgg gtgtgattgg ctcctgaatt tcattatttg cgtttagcaa
     1981 tttctcttgc aatggcgttt catttatttt atagtgcaat cacaatcaca tgggtgccat
     2041 aacgacaacc ggatcaattc gctcgggaga gcactcgaag gcgcggtttc gtctcccgct
     2101 cctcggcctc ctcgcccttc taggcccatt cgcattcgac ttgcgggtgg gccttctttc
     2161 tggcctctat tccctcattc ttatcggcta cgcgctttgg gcgcttcggt tgaccgtggt
     2221 ctttcgggac gacgcgggcc tcggctatct tcttgccctc ttcgacgtgg cgctgacgct
     2281 ccccctcgtg ctgtgggggc ggccgtcttg ggcgctggct cccttggtgg gcgcgtgggt
     2341 agcggggctg acggc
//
LOCUS       DASRMJ010000046         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_438973, whole genome shotgun sequence.
ACCESSION   DASRMJ010000046 DASRMJ010000000
VERSION     DASRMJ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438973"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..649)
                     /locus_tag="VFD74_01400"
     CDS             complement(92..649)
                     /locus_tag="VFD74_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK48242.1"
                     /translation="MDVRAAIIKRRSIRKFEVGREVSDADLAIILEAGRQSQSWKNCQ
                     PWRFVVVRDAELREKIATCLPESNSAVHAVRAASAVLALIAVPAEGEVHQDKPFWMVD
                     CGIAGENMCLQAVELGISTVWISLLDGPRLCGHLGLPEGMECVGIFPLGYAPEGYEPR
                     PRGRKELADIAWSETFGRPFTPAES"
     gene            complement(732..1646)
                     /locus_tag="VFD74_01405"
     CDS             complement(732..1646)
                     /locus_tag="VFD74_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZK48243.1"
                     /translation="MRRVLAIVNPASNNGTTARFWPKAKRVLEQGGIHLDSVLTEGPG
                     HAVALAERAAEDRFEVVLYVGGDGTANEVANGLLRVPAAGRPALAALPRGTGGDFPKS
                     MGIQPGAKAAAARLLAGSERIIDVASCSFVSLDGQHTRRHYLNIADVGLGGYVAERVN
                     RTTKVFGGFASFLYSTVATFWNLDKPEVSVEIDGKVIFEGPATTVAVCNGPRFGGGML
                     MAPGARPDDGVLDVVVIGDIGKVDLALNLPRLYRGTHLTHRKVHAFRGAEVKVTTSVR
                     IPLEIDGEHPGTTPFHVWVEPGALRIVV"
     gene            1764..2216
                     /locus_tag="VFD74_01410"
     CDS             1764..2216
                     /locus_tag="VFD74_01410"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZK48244.1"
                     /translation="MTHKREDQAESALRAARGAGEAGAPAAGGSPAHDSAVAGPVAGS
                     SEAKVELSYYSAPDCHLCEIGLAHIARLADELGLSVRVVDISSGPDLEGRYRSRIPVG
                     EIQGRVVFKYQVDEDRLRRLVEWARVTGESAGAMSAGETSTAGSDRAE"
     gene            2339..3493
                     /locus_tag="VFD74_01415"
     CDS             2339..3493
                     /locus_tag="VFD74_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZK48245.1"
                     /translation="MGSIFGRRANIAAIVIAGAIFFTALGFFAASGIDRPNDSKAAGL
                     WQDGTGLTAEMAAVPSFAPIAEKMRPTVVHILVNGKRSTADLTPDSNPDNPDGGNGLP
                     ESHPPLPPEFEQTSEGSGVIISADGYILTNQHVIADADTITVTLVDGRSFDGRVVGSD
                     GRDDLALVKIEPDTDLQLAPLGDSDKIRAGEWVMAMGNPLGFEYSVTVGVVSGKGREL
                     PSTNFADFIQTDAAIYPGNSGGPLFNLAGEVIGINTAVIPDTNLGFAVPINSAKEILP
                     QLLEKGRVVRGYLGVSIQPVEYMQDPPSGVSEGAAIAQVSEGTPAANAGLAVGDVIVA
                     FDGTPVASPRGLTSLVTATSPGTRVPVSVVRGADRIEVDVVVGSLPTDLE"
     gene            3503..4126
                     /locus_tag="VFD74_01420"
     CDS             3503..4126
                     /locus_tag="VFD74_01420"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZK48246.1"
                     /translation="MGFLEDWIIPLLVDYGLVAIFISQTLESMCIPIPSEVVVPYGGF
                     LAAQGHVALWEVVVVAIAANLLGSTIAYMAGRYGGRALVLRYGRYVFMSEHHLDVADR
                     WFERRGEITVFLTRMMPGIRTFISVPAGVSRMPFGRFVVYSVLGTAPWNVALAYLGWV
                     FGENWGRLQEWLHQYNLVFFALLAVLVAAAVVWKWRDVRKRKSPRAS"
     gene            complement(4220..4942)
                     /locus_tag="VFD74_01425"
     CDS             complement(4220..4942)
                     /locus_tag="VFD74_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZK48247.1"
                     /translation="MIELKGVHKNYGALEVLRGIDLQITKSEVVVVIGPSGSGKSTML
                     RCMNALEPIQKGTITIEGIDITNPKTNVNKVRQKIGMVFQSFNLFPHKSAIENVMLAP
                     LKVLKKSRSEAETIARDLLVTVGLSEKADAYPDQLSGGQQQRVAIARALAMQPDMMLF
                     DEVTSALDPELVAEVLNVMKELALGGMTMVVVTHEMGFAREVGTRMIFMDGGVIVEES
                     DPREMIANPKEERTKKFLGMVL"
BASE COUNT          807 a         1686 c         1734 g          897 t
ORIGIN      
        1 tcagcggtct ttgaccgcgc ccagtgcgcc cggggtgcac cctgggtcag gtgatattcc
       61 cgtcagatgc gcccctcggc ggtgcgacga gctacgactc cgccggggtg aaggggcgac
      121 cgaaggtctc ggaccaggcg atgtcggcga gttccttgcg cccgcgcggc ctcggttcgt
      181 agccctcagg agcgtagccg agcgggaata tccctacgca ctccatgcct tcgggaaggc
      241 caaggtgacc gcagagcctg gggccgtcga gcagcgagat ccagaccgtc gagatgccga
      301 gctcgaccgc ctggagacac atgttctcgc cggcgatgcc gcagtcgacc atccagaacg
      361 gcttgtcctg gtggacctca ccctcggccg gcacggcgat gagtgcgagg acggccgatg
      421 ccgctcggac cgcgtggacg gctgagttgc tctcgggcag acaggtggcg atcttctcgc
      481 gcaactcggc gtctcgcacg acgacgaacc gccagggttg gcagttcttc cacgattgcg
      541 actggcgtcc ggcttcgagg atgatcgcga ggtcggcatc gctgacttct cgacccacct
      601 cgaacttcct gatgctgcgc cgcttgatga tggctgctct cacatccacg gatggtcgct
      661 cccttcttct ctcttcattg accccgccgg ggcggagtca gcgtcttgat ccgacatctc
      721 ggcctccgag tctacaccac gatccgtagc gccccaggct ccacccacac atggaacggc
      781 gtggtgcccg ggtgctcacc gtcgatctcc agcgggatgc gtacggatgt cgtgaccttg
      841 acctccgccc cccggaacgc gtgcaccttt cggtgcgtga gatgcgtccc tctgtagaga
      901 cgcggcaggt tgagggcgag atcgaccttg ccgatgtctc cgatcaccac aacgtcgagc
      961 actccgtcgt cgggtcgtgc tccgggagcc atgagcatcc ctcctccgaa gcgaggcccg
     1021 ttgcacactg ccacggtagt cgcgggccct tcgaagatga ctttgccgtc gatctccacc
     1081 gagacctcgg gcttgtcgag attccagaag gtcgccaccg tcgagtagag gaaagaggcg
     1141 aagcccccga ataccttggt cgttcgattc acacgctctg ccacgtagcc acccagaccc
     1201 acgtcggcga tgttgaggta gtggcgtcgg gtgtgctgcc cgtccagcga gacgaagctg
     1261 caggaggcca cgtcgatgat gcgctcgctg ccggccagga gtcgggctgc ggccgccttc
     1321 gcgcccggct gtatccccat gctcttgggg aagtcgccgc cggtgcccct cggcagtgcg
     1381 gcgagggctg ggcggcccgc cgccggaacg cggagcagcc cgttggcgac ctcgttcgcg
     1441 gtgccgtccc cgccgacgta cagcactact tcgaatcgat cctctgcggc gcgttcggcc
     1501 aacgcgacgg cgtggccggg cccctctgtg aggaccgaat ccaggtggat gccgccctgt
     1561 tcgagcaccc gtttggcttt gggccagaat ctggccgtgg ttccgttgtt ggaggccgga
     1621 ttcacgatgg caaggacgcg acgcatggag gcagtaaagc agatacggtg gccgcgcggt
     1681 agactcccag gcggacgggc cgcgcgcggc gggacgcggg tggtgctaca aggggcggga
     1741 caacctcgga ccgaggtcgg cacatgacgc acaaaagaga agatcaggct gagagcgcgc
     1801 tccgcgctgc gcggggcgcc ggggaggcgg gcgcccccgc ggccggcggc tcgccggccc
     1861 acgactccgc cgtcgccggc ccggtggccg gcagctccga ggcgaaggtc gagctgtcct
     1921 actactccgc gccggactgc cacctgtgcg agatcggtct ggctcacatc gcccggttgg
     1981 ccgacgaact cggcctatcg gtgcgcgtcg tggacatctc cagcggtccc gacctcgagg
     2041 ggcgctaccg cagccggatc ccggtggggg agatacaggg tcgcgtggtc ttcaagtatc
     2101 aggtggatga ggaccgtctg cgtcggttgg tggagtgggc gcgggtcacc ggggagtccg
     2161 ccggcgcgat gtccgcgggc gagacgtcca ctgcggggtc cgaccgcgcc gagtgacggg
     2221 cgcgtaggcg tcggcgccgg gaagacgcga gtataatcgc ccgagaccat cctctgcagc
     2281 ggtcgtccgg ttggacgccg cgtcagcagt cccaccacac agcgaacagg gagagacaat
     2341 gggatcgatc ttcgggcggc gcgccaacat cgccgccatc gtcatagccg gggccatctt
     2401 cttcaccgcc ctgggcttct tcgcggccag tggcatcgat agaccgaacg acagcaaggc
     2461 cgccggtctc tggcaggacg gcaccggcct gacggccgag atggccgccg tccccagctt
     2521 cgcaccgatc gccgagaaga tgcggcccac cgtcgtccac atcctcgtca acggcaagcg
     2581 gtcgaccgcg gacctcactc ccgatagcaa ccccgacaat ccggacggtg ggaatggtct
     2641 cccggagtcg caccctccgc tgccacccga gttcgagcag acatccgagg gctccggggt
     2701 catcatcagc gctgacggat acatcctcac caaccaacat gtcatcgccg acgccgacac
     2761 catcacggtg acgctcgtcg acgggcgttc cttcgacggg agagtcgtcg ggagcgatgg
     2821 gcgtgacgac ctcgcgctgg tcaagatcga gcccgacacg gacctgcagc tcgccccctt
     2881 gggcgattcg gacaagatcc gtgccggcga gtgggtcatg gcgatgggca acccgctggg
     2941 cttcgagtac tcggtgaccg tgggtgtggt gagcgggaag gggcgggagc ttccctccac
     3001 gaacttcgcc gacttcatcc aaacagacgc cgccatctac ccaggtaaca gcggaggccc
     3061 gctgttcaac ttggccggcg aggtcatcgg catcaacacc gctgtgatcc cggataccaa
     3121 cctcggcttc gcggtgccga tcaacagcgc caaggagatc ctgccgcaac tgctcgagaa
     3181 ggggcgcgtg gtgcgcggct acctgggggt cagcatccag ccggtggagt acatgcagga
     3241 tcctccgagc ggggtcagcg agggagccgc gatcgcgcag gtgagcgagg gcacgccggc
     3301 cgcgaacgcc ggtctggcgg tcggtgacgt gatcgtggcc ttcgacggca caccggtcgc
     3361 gtcaccgcgc gggctcacct cgctggtcac cgcgacgagt ccgggaacgc gagtgcccgt
     3421 gtcggtggtg cgcggcgcgg accgcatcga agtggatgtc gtggtggggt cgctccccac
     3481 ggacttggag tagccgcagc ccatgggttt cctcgaagac tggatcatcc cgctgctggt
     3541 ggactacggc ctggtggcga tcttcatcag ccagacgctg gagagcatgt gcatccccat
     3601 cccgagcgag gtggtggtgc catacggcgg tttcctggcc gcccaggggc acgtggctct
     3661 ctgggaggtg gtggtcgtgg cgatcgcggc caacctgctg ggatccacca tcgcctacat
     3721 ggcgggccgg tacggagggc gcgccctcgt cctgcgctac ggcagatacg tgttcatgtc
     3781 ggaacatcac ctcgatgtcg ccgaccgttg gttcgagcgg cggggcgaga tcacggtgtt
     3841 cctcacgcgg atgatgcccg gcatccgcac cttcatctcg gtgcccgccg gtgtgagtcg
     3901 catgcccttc gggcgcttcg tcgtctactc ggtcttgggc acggctccgt ggaacgtggc
     3961 gctcgcgtac ctgggctggg tgttcggcga gaactggggt cggctgcagg agtggcttca
     4021 ccagtacaac ctggtgttct tcgccctcct cgccgtgctg gtggcagcag ccgtcgtctg
     4081 gaagtggcgt gacgtgcgaa agcgaaagtc gccccgggcc tcgtgacgca cgacgatggg
     4141 cgccgagtcg gccgactcgg ccgggccgtg ccgcgggccc cggaggcgca gccgacggcg
     4201 ggccggtgcc cgccctagcc tagagcacca tccccaagaa cttcttggtg cgctcttcct
     4261 tcgggttcgc gatcatctcg cgcgggtcgc tctcctcgac gatcacgccg ccgtccatga
     4321 agatcatgcg ggtgcccacc tcgcgggcga agcccatctc gtgggtgact accaccatgg
     4381 tcatgccgcc gagcgcgagc tccttcatga cgttgagcac ttcggccacc agttcggggt
     4441 cgagggccga ggtgacctcg tcgaagagca tcatgtcggg ctgcatcgcg agggcccggg
     4501 cgatggcgac gcgctgctgc tgccctccgg agagttggtc ggggtaggcg tcggccttct
     4561 ccgacagacc tacggtgacg aggaggtcgc gggcgatcgt ctcggcctcg ctacgcgact
     4621 tcttcagcac tttgagcggg gccagcatca cgttctcgat cgccgacttg tgcgggaaga
     4681 ggttgaagct ctggaagacc atcccgatct tctggcgcac cttgttcacg ttcgtcttcg
     4741 ggttggtgat gtcgatgccc tcgatggtga tggtgccctt ctggatcggt tcgagggcgt
     4801 tcatgcagcg gagcatcgtg gactttcccg agccggacgg tccgatcacc acgaccacct
     4861 cgctcttggt gatctgcaga tcgatgcccc gcagcacctc aagggccccg tagttcttgt
     4921 gcacgccctt cagttcgatc attacctcgg agctcatcct agacctccgc ctttcgggag
     4981 ttgagccgcg acgtcgggga gcaccggcgc ggacgtcttg ccggtggaac cacccgtggg
     5041 cttggctccc ttggaactac cttcccgctt cgtctcgagg cgcgaggcca atcggatcag
     5101 tgggatggtg aagagcacat acac
//
LOCUS       DASRMJ010000047         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_473748, whole genome shotgun sequence.
ACCESSION   DASRMJ010000047 DASRMJ010000000
VERSION     DASRMJ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473748"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..2915
                     /gene="uvrA"
                     /locus_tag="VFD74_01430"
     CDS             3..2915
                     /gene="uvrA"
                     /locus_tag="VFD74_01430"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZK48248.1"
                     /translation="MVIVGAREHNLKDISLRLPRDRFIVITGLSGSGKSSLAFDTIYA
                     EGQRRYVESLSSYARQFLGQMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVGTVTEIH
                     DYLRLLYARIGHPHCWICGRPIEGQSKGQIIDQILGLEPGTKFVVRAPLVRGQKGWHK
                     EALEHVRREGFTRIEIDGVQYTMDGELPELDKNVRHDVSIVVDRLVMKEGVRRRLSDS
                     VETALAEGQGLLEVDVIEGEGRTILVFSENLACPEHGVSLPEMAPRIFSFNSPHGACE
                     TCSGLGSRREIDPSLIVGNPDRSIAEGVLLPWSTTSSQYYEQIITALAEKHEIEVDAP
                     WRSLSEEVQSAFLFGTGKERLYVSYVNRRGLKRSYMTTFEGIVPNLERRYRETDSEYM
                     REKIEEYMSEVPCPACKGARLKPQSLAVTVGGLNLHELSVLPVTESVGFFDRLELSER
                     ERLIGGRVLKEILERLSFLESVGVGYLDLHRTAGTLSGGEAQRIRLATQIGSSLVGVL
                     YILDEPSIGLHQRDNRKLIETLLRLRDLGNTVIVVEHDEETMLAADYLVDMGPGAGEH
                     GGEVVACGSVEEVLAEPRSITGQFLVGRISVPVPASRRAGRGHFWVRGARQHNLRGID
                     VSVPTGALVCVTGVSGSGKSTLVNEIIYKSLSNLVNRITVRPGAHDSVEGIEQLDKVI
                     DIDQSPIGRTPRSNPATYTGLFDHIRQLFAQTKESKVRGYKPGRFSFNVKGGRCESCK
                     GDGTLKIEMHFLPDIYVPCEVCGGRRYDRETLEVHFKGKNIADVLAMSVEEAVVFFEN
                     QPKIRRRLQTLNDVGLGYIKLGQPSTTLSGGEAQRVKLATELSKVATGNTLYILDEPT
                     TGLHFADVAKLLEVLQRLVDSGNTVLVIEHNLDVIKSADWIIDLGPEGGSAGGELIAE
                     GTPEEVADNPRSYTGYYLRRVLPGFHDGPPRAPAEAGPGDTDEVPSAGREVGAASI"
     gene            complement(3174..>3566)
                     /locus_tag="VFD74_01435"
     CDS             complement(3174..>3566)
                     /locus_tag="VFD74_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HZK48249.1"
                     /translation="LFLNKGCTREEIVRDAHAFYLTKGWLCHDNPVRDSLLEWSRRFG
                     PEKARELRKATLSAYERVTLIHTGAFDVAATLPAGRTFADELELPLAHTSGSVVLLER
                     LFAGPWDSEIVVVSPGEPICLLHLFESQ"
BASE COUNT          654 a         1142 c         1184 g          586 t
ORIGIN      
        1 agatcgtcat cgtcggcgcg cgggaacaca acctcaagga catctccctc aggctcccgc
       61 gcgaccgttt catagtcata accggcctct ccggatcggg taagtcgagc ctcgccttcg
      121 ataccatcta cgccgaaggt caacgccgct acgtggagag cctgtcgtcg tacgcccgcc
      181 agttcctcgg gcagatggac aagccggacg tcgactccat agagggcttg tccccggcca
      241 tctccatcga ccagaagaca acgtcgcgca atccccggtc cacggtgggg acggtcacgg
      301 agatccacga ctacctccgc ctgctctacg cccgcatcgg ccatcctcac tgctggatct
      361 gcggacgccc catcgaaggg cagagcaagg gccagatcat cgaccagatc ctgggactcg
      421 agcccggcac caagttcgtg gtgcgggcgc cgctggtgcg cggccagaag gggtggcaca
      481 aggaagccct ggagcatgtg cgtcgggagg gcttcacccg catcgagatc gacggcgtgc
      541 agtacaccat ggacggcgag ctacccgaac ttgacaagaa cgtgcgccat gacgtgagca
      601 tcgtcgtgga ccggctcgtg atgaaggagg gtgtgcggcg acgcctgtcg gattcggtgg
      661 agacggccct ggccgaaggg cagggcctgc tcgaggtcga tgtcatcgag ggcgagggcc
      721 gcacgatcct ggtgttctcc gagaatctgg cctgccccga gcacggtgtg tcccttcccg
      781 agatggcgcc gcgcatcttc tcgttcaact cgcctcacgg cgcctgcgag acctgcagcg
      841 gcctgggctc gcgccgtgag atcgacccgt cactgatcgt cggcaacccc gaccgctcca
      901 tcgccgaggg cgtccttctt ccctggagca ccacctcgtc ccagtattac gagcagatca
      961 tcacggccct cgccgagaag cacgagatcg aggtggacgc cccgtggcgc tccttgtctg
     1021 aggaagtgca gagcgccttc ctgttcggca ccggcaagga gcggttgtac gtgtcgtacg
     1081 tcaatcggcg cgggctcaag cgcagctaca tgaccacttt cgagggcatc gtgccgaatc
     1141 tggagcgccg ctaccgcgag accgactccg agtacatgcg cgagaagatc gaagagtaca
     1201 tgtcggaggt gccgtgcccg gcctgcaagg gcgcgcgcct caagccccag agcttggcgg
     1261 tcacggtggg agggctcaat cttcacgagc tcagcgtcct ccccgtgacc gaatcggtgg
     1321 ggttcttcga ccgcttggaa ctgagcgagc gagagcggct tatcggcggg cgggtgctca
     1381 aggagatcct ggaacggctc tcgttcctcg aatcggtggg ggtgggctac ctcgacctgc
     1441 accgcaccgc gggtaccctc tccggcggcg aagcacaacg catccgcctg gccacgcaga
     1501 ttgggtccag cttggtcggc gtgctctaca tcctggacga gccttctatc gggctccatc
     1561 agcgggataa ccggaagttg atcgagaccc tcctccgtct gcgcgacctc ggcaacaccg
     1621 tgatcgtggt ggagcacgac gaggagacca tgctcgccgc cgactacctc gtggacatgg
     1681 ggcccggcgc gggagagcat ggcggcgagg tcgtggcgtg cgggtccgtg gaagaggtgc
     1741 tggcggagcc gcgctccatc accggtcagt tcctcgtcgg gcgcatctcc gtccccgtgc
     1801 cggcctcccg ccgcgccggg cgcggacact tctgggtgcg tggcgcccgg cagcacaacc
     1861 tgcgtggcat cgacgtgagt gtgcccacgg gcgctctggt gtgcgtcacc ggggtgtcgg
     1921 gatcagggaa gtccaccctc gtcaacgaga tcatctacaa gagcctgtcg aacttggtca
     1981 accgcatcac ggtgcggccg ggcgcgcacg actccgtgga agggatcgag cagctggaca
     2041 aggtgatcga catcgatcag tcgcccatcg gacgcacgcc ccgctcgaat cccgccacct
     2101 acaccggcct gttcgaccac atccgacaac tcttcgcgca gaccaaggag tcgaaagtcc
     2161 gcggctacaa gccggggcgg ttcagcttca acgtgaaggg cggacgctgc gagtcgtgca
     2221 agggcgacgg caccctcaag atcgagatgc acttcctccc cgacatctac gtgccctgcg
     2281 aggtgtgcgg gggcaggcgc tacgaccgtg agaccctcga ggtgcacttc aagggcaaga
     2341 acatcgcgga cgtgctggcc atgagcgtcg aagaggccgt cgtgttcttc gagaaccagc
     2401 cgaagatccg caggcgcctg cagaccctga acgacgtcgg cctcggctat atcaagttgg
     2461 gccagccctc cacgacgttg tcgggggggg aagcgcagcg ggtcaagctg gcgacggagc
     2521 tctcgaaagt ggctaccggt aacaccctct acatactcga tgagcccacg acgggtctcc
     2581 acttcgccga cgtggctaag ttgttggagg ttttgcagcg gctggtggat tcaggcaaca
     2641 cggtgttggt gatcgagcac aacctcgacg tgatcaagtc cgcggactgg atcatcgacc
     2701 tggggcctga gggtgggtcg gccggagggg aactcatcgc ggaggggact cccgaggagg
     2761 tggccgacaa tcctcggagc tacaccggat actacttgcg ccgggtgctc cccggcttcc
     2821 atgacggccc gcctcgcgca cccgccgagg ccgggcccgg cgacaccgac gaagtgccct
     2881 ccgccggccg ggaagtcgga gccgcatcga tctgagcggc gcccgccgcc tcggcggccg
     2941 ccatccccaa gacccgtagg tctgcgccgg cgccactggg agcgctccca ccgaaccgtg
     3001 tgatgtcgac gttcaggaaa gcgtgcgcga tcgacacgga gaacatgagg gagcccacga
     3061 agaagccgaa ggcggtccag tagagagtcg tcatgtctgt atctcccgac gttcgcgtgc
     3121 caacatccca gatccacgtc cgaaggattg atcatggtgc gaaactgcgg ggactactgc
     3181 gactcgaaca gatggagaag gcatatgggc tctccggggg acaccaccac gatctccgag
     3241 tcccagggtc cggcgaacaa gcgttccagc aggaccacgg atcctgaggt gtgggcgagg
     3301 gggagttcca gttcgtcggc gaaggtgcgg ccggcgggca gcgtggccgc gacgtcgaag
     3361 gcgccggtgt ggatgagggt gacgcgctcg taggcggaca aggtggcctt tcgaagctct
     3421 cgggccttct cgggcccgaa acgcctgctc cattcgagta gggagtcccg caccgggtta
     3481 tcgtgacaga gccagccttt ggtgaggtag aaggcgtggg cgtcacgaac gatctcctca
     3541 cgggtgcacc ccttgttcag gaacag
//
LOCUS       DASRMJ010000048         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_147854, whole genome shotgun sequence.
ACCESSION   DASRMJ010000048 DASRMJ010000000
VERSION     DASRMJ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147854"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..>2030)
                     /locus_tag="VFD74_01440"
     CDS             complement(55..>2030)
                     /locus_tag="VFD74_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK48250.1"
                     /translation="LGMAMVTAVIVTWVADPAVLRSASWMRLAEAVVFVIAVTGLTWL
                     VFGPLTVAEHPVLRTYMLLPLMAWIAFRFSPRDAAGTLLLIAIVALWNTRLGYGIFAS
                     AADSPSERLVEVQTFLMVLTLLGLLLSVEISERRETTEALRRSEEGLKRAQRHARVGS
                     WTWDVKGDRLEWSDEMFRIFGLRRETFTGSLQAVIAEAIHPEDRAAVERSNLSVVRDR
                     RPIPLEYRVVRPDGSVRVVWAEAGEFLPDEHGDPLLLSGTVRDITEQKEAEANKARLE
                     SQLQHAIKMESVGRLAGGVAHDFNNMLGVILGHMELALEQVDSSHPVHADLIATQQAA
                     QRSAHLTRQLLAFARKQAVVPRVLDLNETVAGMLTMLERLIGEDIEISFSPAGGLWPV
                     SMDPSQIDQILANLCVNARDAIAGTGCISIETTNESLDEDHCVEHPGCIPGDYVRLVV
                     ADDGRGMDEETRRHLFEPFFTTKNTGEGTGLGLATVYGIVEQNGGFIEVESEAGEGCT
                     FTIYLPRHVGDAPPADTGEPRGSSTRGREIILVVEDEPAILDLATRMLRRQGYIVLAA
                     ATPAQAIRLAEERQGDIDLLVTDVVMPEMNGRDLADTLRTYAPRMRRLFMSGYTADVI
                     ASRGVLDDGVNFLEKPFTAQGFAAAVREVLSRE"
BASE COUNT          367 a          676 c          632 g          355 t
ORIGIN      
        1 gatccgggag ctgccgctgg gcgcatgaac gctgaggcgc acccccaccg atccctactc
       61 tcggctcagc acctcccgca ccgccgcggc gaaaccctgg gcggtgaagg gcttctcgag
      121 gaagttcacg ccgtcatcca ggacgccgcg ggaggcgatg acgtcggccg tgtagcctga
      181 catgaagaga cggcgcatgc gaggagcgta ggtccgcagc gtgtccgcca aatcgcgccc
      241 gttcatctcg ggcatgacca catccgtcac gagcagatcg atgtcgccct ggcgctcttc
      301 cgccaggcga atagcctgag ccggagtggc cgccgccagg acgatgtagc cctgccgcct
      361 cagcatcctg gtggccagat cgaggatggc gggctcgtcc tcgacgacga gaataatctc
      421 acgtcctcgg gtagacgagc ctctgggttc acctgtgtcc gccggtggag cgtcgccgac
      481 gtgcctggga aggtagatcg tgaacgtgca cccctcgccc gcctcgctct cgacctcgat
      541 gaagccgccg ttctgttcga cgatgccgta gacagtggcg aggcccaacc ctgtgccctc
      601 gcccgtgttc ttggtcgtga agaacggctc gaagaggtgt ctccgcgtct cctcgtccat
      661 gcccctgccg tcgtcggcca cgacaagacg aacatagtct ccggggatgc agcccggatg
      721 ttcgacacag tgatcctcgt cgagggattc gttggtggtc tcgatggaga tgcagcctgt
      781 gcctgcgata gcgtcacgag cgttcacaca caggttggcc aagatctggt cgatctgaga
      841 cgggtccatg ctgacgggcc acaatccacc cgcgggcgag aaggagatct ctatgtcctc
      901 accgatgagg cgctcgagca tggtcaacat gcccgcaacg gtttcgttga ggtctaggac
      961 gcggggaacg acggcctgtt tgcgggcgaa cgccagcaac tgtcgggtga gatgggcgga
     1021 tcgttgggcg gcttgctgag tcgcgatgag gtctgcatgc acgggatggg aggagtccac
     1081 ctgctccagc gccagctcca tgtggcccag gatcacgccc agcatgttgt tgaagtcgtg
     1141 cgccacgccg cctgccagac gacccaccga ctccatcttg atggcatgct ggagttgaga
     1201 ttcgagccgg gccttgttcg cctcggcctc cttctgttcg gtgatgtcgc gcacggtccc
     1261 gctgagaagg aggggatctc cgtgctcatc cggaaggaac tcgcccgcct ccgcccacac
     1321 cacgcggacc gatccatccg gccgcaccac ccgatactcc agcggtatgg gacgcctgtc
     1381 ccggaccacc gacaggttcg aacgctcgac cgcggcgcga tcttcaggat gtatggcttc
     1441 tgcgatcacc gcctgcaacg aacctgtgaa cgtctccctt ctcaggccga agatgcggaa
     1501 catctcgtcc gaccactcga gtcgatctcc cttcacgtcc cacgtccagc tgcccacacg
     1561 ggcgtgccgc tgcgccctct tcagcccttc ctcactcctc cgcagcgctt cggtcgtctc
     1621 tctgcgctcg gagatctcca cgctcaagag caggcccagg agggtcagga ccatgaggaa
     1681 cgtctgcacc tcaaccaggc gttcggacgg ggagtcggct gcagaagcga agatgccgta
     1741 gcccagcctc gtgttccata gagcgacgat ggcgatcagc agcagggtgc cggcggcgtc
     1801 cctgggactg aatctgaagg cgatccacgc catcagcgga agcaacatgt aggtacgcaa
     1861 caccggatgc tcggcgaccg tgagcggccc gaagacgagc cacgtgagac ccgtcaccgc
     1921 gatcacgaac acgactgctt ccgccagtcg catccaggat gcggatctga gcaccgcggg
     1981 gtcggcgacc caggtgacga tgaccgccgt gaccatggcc atcccgagcc
//
LOCUS       DASRMJ010000049         4451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_78674, whole genome shotgun sequence.
ACCESSION   DASRMJ010000049 DASRMJ010000000
VERSION     DASRMJ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78674"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFD74_01445"
     CDS             complement(<1..790)
                     /locus_tag="VFD74_01445"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48251.1"
                     /translation="MALSPPPSAGAPVPGPVAGSLGLEVHGLSHGYGGRLVLDQIDLT
                     VAAGSLTVLAGPSGCGKSTLLRLIAGLEVPESGRVLLGGEDLTGRPGRVGLVFQEAAL
                     YPWLTVAGNVSFGLHIQGHARGDISARAGEVLDLVGLTEAARMYPHQLSGGMAQRAAL
                     ARALVAFPSLLLLDEPFGSVDLHLRSRLQDEVIRLWQATGSTIILVTHSVDEAVLVGE
                     RVVLMSSAPGRLTGDVDVPLPFPRDRKDPHFLRLRDEVFEIGRRT"
     gene            complement(781..1557)
                     /locus_tag="VFD74_01450"
     CDS             complement(781..1557)
                     /locus_tag="VFD74_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK48252.1"
                     /translation="MGRGVRESAAALASFAVVGLAWQAVVALGWLDLPGPVAVARTFL
                     SLLVTPDPVFGKTIWEMVGLSLWIVLRGGLVGVLAGLPTGLVLGAFPRIRPALRPLLG
                     VLQPIPPLAWLPLAYLVFAGAARPSVWVQLFVVALAVFFPVTWATFQGVEYSPPVFAM
                     VARTFGATSRQLLFRVSLPAALPALMSGIRVGVGVGWMSIIAAEFVGGRTGIGFFVWN
                     AYSLGGRSAQVMAGILAIGLTGLAMNYLLLLLEKKVTPWH"
     gene            complement(1571..2605)
                     /locus_tag="VFD74_01455"
     CDS             complement(1571..2605)
                     /locus_tag="VFD74_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK48253.1"
                     /translation="MRRTALRALGALMVLSAGLILAGCGAGEEPADFVTIVQIAPASM
                     EDALRSGEIDGFLAWEPFPVRAVQAGGGEYLASSKDIWPDHPCCVLAADRGLTDRQTI
                     DALLWVHLQATDFLVDPANREKVVEYGVEFTGTPEVVVREALGRLTYSADDPQAGLRR
                     LAGSLEGQAMIPAATGEDFWTSFVATSETGVVRERMAAEPTWVPTVGPGTVRIGYIAS
                     GDLHQLALHVGIREGLFEKAGLVLGQNLALRPYPNGVAIMQAFAAGELDVAYLGVAPA
                     LLKSINDRVPIAVLAGANQEGSSLVVRPGIERVEDLAGLRVAVPQVGTVQHILLLELL
                     ERYGLQPRLG"
     gene            complement(2605..3660)
                     /locus_tag="VFD74_01460"
     CDS             complement(2605..3660)
                     /locus_tag="VFD74_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HZK48254.1"
                     /translation="MGAESAPDGLLMGGSSFETVLAHAAGEGLLREVRSSLVASAGLA
                     RKREIGQAVRALAGGYFPLDWPPCFGEDAAAIPHGDGYLLLAADGIRQDLLTDPFWAG
                     YCSVLVNVNDVVGTGGRPLAMVNVVSGADAGRREELLSGVAFGSRHFGVPMIGGHLHP
                     EGTEESVAVAIVGTARSLVSSFAARPGDELVVAVDTQGSWHGAFPHWDSTSQRSPEEL
                     QAQLSVLPRLCEGGRVLAGKDISNPGLLGTLLMLLESSGVGAVVDLERVPAPPGVPLV
                     SWLEAYPGLGFVLAVSPDRSGEVIEAFSAAGMAAAVAGGFTAGSVLAARCAGSESPLW
                     DHSITPVTGVATTMETF"
     gene            complement(3627..4088)
                     /locus_tag="VFD74_01465"
     CDS             complement(3627..4088)
                     /locus_tag="VFD74_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HZK48255.1"
                     /translation="MSEREIVVAQKGHIRDAAFDVRQQVFCQEQGLFDGSDLDAHDEG
                     AIHLAALADGLVVGTVRLYEREPGVWIGGRLAVLRGHRGGTGFRLVQRAVEEARRRGA
                     SVFLASVQSPNEPFFRRLGWHTVAEGQELRGMPHVIMEAPLWVRSQRRMGS"
     gene            complement(4085..>4451)
                     /locus_tag="VFD74_01470"
     CDS             complement(4085..>4451)
                     /locus_tag="VFD74_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK48256.1"
                     /translation="GWEGFLRLWREAVEVFGPWQVVTYVIAGLGEDLETTLQRLAEAV
                     AVGVYPHVVPFRPVPGAELAHLSPPEPALMERLYRETARMLRAAGGSWRRIEAGCGRC
                     TACSALPDFEEELSLGSVG"
BASE COUNT          818 a         1604 c         1454 g          575 t
ORIGIN      
        1 gagtccgcct gccgatctcg aagacctcgt cgcgcaggcg gaggaagtgg gggtccttgc
       61 ggtcacgtgg gaaggggagc ggcacgtcca cgtcgccggt cagcctcccc ggggccgagg
      121 acatcagcac tacgcgctcg cccaccagca ccgcttcgtc aacgctgtgc gtgacgagga
      181 tgatcgtgct gcccgtcgcc tgccacaggc ggatgacttc gtcctggagt cggcttcgca
      241 gatgcagatc gaccgaaccg aagggctcgt cgagcaggag gagcgaagga aaggcgacga
      301 gcgctcgagc gagcgcggct ctctgagcca ttccgcccga gagttggtgc ggatacatgc
      361 gggcggcctc ggtcaacccc accagatcaa gtacctctcc ggcgcgagcg gatatgtcgc
      421 cccgagcgtg accctggatg tgaaggccga acgagacgtt ccccgccacc gtgagccagg
      481 ggtagagggc ggcttcctgg aagacgagtc ccaccctgcc cgggcggccg gtgaggtcct
      541 ccccgccgag cagcacccgc ccgctctccg gcacctccaa ccccgcgatg agtcgcagca
      601 gagtgctctt gccacagccg ctgggcccgg cgagcaccgt gaggctgccc gcggccaccg
      661 tgagatctat ctggtcaagc accagtcggc caccgtagcc gtgcgaaagg ccgtggacct
      721 cgaggccgag gcttcccgcg acggggccgg gcacgggggc gccggcactc ggcggcggac
      781 tcagtgccac ggtgtcacct tcttctcgag gagcagcagc aggtagttca tagccagacc
      841 ggtgagaccg atcgcgagga tcccggccat gacctgcgcg cttcggccgc ccaacgagta
      901 cgcgttccag acgaagaacc cgatgcccgt ccttcccccg acgaactccg ccgcgatgat
      961 cgacatccac cccacgccca cgcccacgcg aatgccactc atgagagcgg gcaaggccgc
     1021 gggtagcgac acccggaaca gcaactgccg agaggtagcc ccgaaggtcc gagccaccat
     1081 ggcgaagaca ggcggactgt actcgacccc ctggaaggtc gcccacgtga cggggaagaa
     1141 cacggcgagc gcgacgacga agagttgcac ccacaccgat gggcgtgcgg cgccggcgaa
     1201 gacgaggtag gcgagcggca gccaggctag tggagggatc ggctgcagca caccaagaag
     1261 aggccgaagc gcggggcgga tccgcgggaa cgcgcccaac acgagacccg tggggaggcc
     1321 ggccagaaca cccacgagtc cgccgcgcag gacgatccac aggctgagtc ccaccatctc
     1381 ccaaatggtc ttgccaaaga ccgggtctgg cgtcaccagc agcgacagga acgtccgggc
     1441 gaccgccacc ggacccggca ggtcgagcca tccgagtgcc acgacggcct gccatgcgag
     1501 tcctacgacg gcgaacgagg ccagagccgc cgccgactcg cggacgcccc gccccaaggc
     1561 ggcccgccga ctagccgagg cggggttgga ggccatagcg ctcgagcagc tccaggagaa
     1621 ggatgtgctg tacggtcccc acttggggca cagccacgcg caggccggcc agatcctcca
     1681 cccgttcgat cccggggcga acgaccagcg acgacccttc ctggttggcc ccggcgagca
     1741 ccgcgatggg cacccggtcg ttgatcgact tgagcagagc cggagccact ccgaggtagg
     1801 ccacgtccag ctcgccggcc gcgaacgcct gcatgatggc gacgccgttg ggatagggac
     1861 ggagcgcgag attctgaccc aggacgaggc ccgccttctc gaacagaccc tcccggatcc
     1921 ccacgtgcag cgccaactgg tggaggtctc cgctggcgat atagccgatg cggacggtgc
     1981 ccggtccgac cgtgggcacc caggtgggct ccgcggccat cctctccctc acgacccccg
     2041 tctcgctcgt ggcgacgaag ctcgtccaga agtcctcacc cgtggcggcc gggatcatcg
     2101 cttgcccttc caggctcccg gccaggcgcc gcagtccggc ctgcggatcg tcggcggagt
     2161 atgtgagtcg gcctagagct tcgcgcacca ccacttccgg agtccccgtg aactcgacgc
     2221 cgtattcgac aaccttctca cggttggcgg ggtcgaccag gaagtcggtg gcctggagat
     2281 ggacccagag cagcgcgtcg atcgtctgtc gatcggtgag accgcggtcg gcggcgagca
     2341 cgcagcaggg atggtccggc cagatatcct tcgaggaggc gagatactcg ccgcccccgg
     2401 cctggacggc gcgcacggga aagggctccc acgccaggaa gccgtcgatc tcgccggaac
     2461 ggagagcgtc ctccatactg gcgggagcga tctggacgat ggtgacgaag tccgcgggtt
     2521 cctcgcccgc tccacacccc gcgaggatca gcccggcgct cagcaccatg agcgcgccga
     2581 gggcacgcag tgcggtgcgt ctcatcagaa cgtctccatt gtcgttgcga cccccgtgac
     2641 gggagtgatg gagtggtccc acaggggtga ctccgaccct gcgcaccgcg ccgccaggac
     2701 actgcccgca gtgaagccgc cggcgacagc cgcggccatg ccggcggccg agaaggcctc
     2761 gatcacctca cccgaacggt ccggggacac ggcaagcacg aacccgaggc cggggtacgc
     2821 ctcgagccac gagaccagcg ggacgcccgg cggggcggga acgcgctcca ggtcgaccac
     2881 ggcgccgacc cctgaactct caagcagcat gagaagcgtt ccgagaaggc cggggttgct
     2941 gatgtccttg cccgcgagca cacggccgcc ttcgcacaag cgaggcagga cgctgagctg
     3001 cgcctgcaac tcctccggag accgctgtga agtggagtcc cagtggggga aggctccgtg
     3061 ccaggatccc tgggtatcga ccgccaccac gagctcgtct cccggacgtg ccgcgaagct
     3121 gctgacaagg cttcgggccg tccccacgat ggccacggcg accgactcct ccgtcccttc
     3181 cgggtgcaga tggcctccga tcatcggcac cccgaagtgc cgggaaccga aagcgacgcc
     3241 cgaaaggagc tcttccctgc ggccggcgtc ggccccggag acaacgttca ccatggcgag
     3301 cggccgtccg ccggtgccca cgacatcgtt cacgttcacg aggacgctgc agtagccggc
     3361 ccagaaaggg tcggtcagaa gatcctgacg gatgccgtcg gcggcaagaa gaaggtatcc
     3421 gtcgccgtgg gggatcgccg ccgcatcctc accgaagcag gggggccaat ccagcggaaa
     3481 gtagcccccg gccagcgctc gcaccgcctg gccaatctcc ctcttgcggg ccaatcccgc
     3541 ggaggcgacg agactcgacc gcacctcgcg gaggagcccc tcgccggcgg catgggcgag
     3601 caccgtctcg aacgacgacc cgcccatcaa gagcccatcc ggcgctgact ccgcacccac
     3661 aaaggcgcct ccatgatcac gtgcggcata ccccgcaact cctggccttc tgccacagta
     3721 tgccacccca atcgcctgaa gaagggctcg ttcggagatt gcacgctggc cagaaacact
     3781 gaggcgcctc ggcgccgcgc ctcctccacc gcacgctgca caagccggaa cccggtcccg
     3841 ccacgatggc cgcgcaacac cgcgagccgc ccgcctatcc acacgccggg ctcccgctcg
     3901 tacagtcgca cggtccccac gaccaagccg tcagcaagtg ccgcaagatg gatcgcgccc
     3961 tcgtcgtggg catcgaggtc ggagccgtcg aacagtccct gctcctggca gaagacctgt
     4021 tgccgaacgt cgaaggccgc gtcgcggatg tgccctttct gggccacgac tatctccctc
     4081 tcgctcatcc cacgctcccg agcgacagct cctcctcgaa atcgggaaga gccgagcagg
     4141 ccgtgcatcg cccgcatcca gcctcgatcc gacgccacga acctcccgcg gcgcggagca
     4201 tccgcgcggt ctcacgatag agtcgctcca tgagcgcggg ttccggcgga gagaggtgtg
     4261 cgagttcggc gccgggcacc ggtcgaaagg gaaccacgtg tgggtagacc ccgacggcca
     4321 cggcctcagc gagtcgctgc agagtcgtct ccagatcctc gccgaggccc gcgatgacgt
     4381 aggtgaccac ctgccacggt ccgaacacct cgaccgcttc gcgccacagc cgaagaaagc
     4441 cctcccaccc c
//
LOCUS       DASRMJ010000050         4863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_256565, whole genome shotgun sequence.
ACCESSION   DASRMJ010000050 DASRMJ010000000
VERSION     DASRMJ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256565"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VFD74_01475"
     CDS             <1..1089
                     /locus_tag="VFD74_01475"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZK48257.1"
                     /translation="CIDNDLVFVGPTPESIDKLGDKSLAKRIMAEAGLPTIPGTEGIL
                     ESVAEATRVAADVGYPVMLKAASGGGGRGMRLVGSPAALERSYQAASAEALAAFGDGG
                     LYLEKVVERPHHVEVQVIGDGRGGVLTLGERDCSIQRRHQKVLEESPSPLLDAEGRAR
                     LLEVATEACSAIRYLSAGTLEFLADEHRDFFFMEMNTRVQVEHPVTEMVTGIDVVREQ
                     IRVASGEPLIATGSVVPNGHAMEFRINAEDPANGFLPRGGVIKRLRLPGGPGVRVDTH
                     LYEGYEVPTNYDSLLAKVIVWDRDRASCIARGLRCLDELEIEGLPTTKDLHLDILRHE
                     RFVAGNISTAYLDEAREELATLRGATPC"
     gene            1101..1511
                     /locus_tag="VFD74_01480"
     CDS             1101..1511
                     /locus_tag="VFD74_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZK48258.1"
                     /translation="MLSMEGSELIVADYRISPAVLRAIVRHSLGGDPRVRLAHGVSRA
                     GRHAVDVAVSEGACAVTVHMQAPLGEDLLALGSKVKEDVETGLAAMTGMPVARVDVHV
                     DGVYRASASTQTATSAVTSTFAPESCDPIPDAAA"
     gene            1508..1900
                     /gene="nusB"
                     /locus_tag="VFD74_01485"
     CDS             1508..1900
                     /gene="nusB"
                     /locus_tag="VFD74_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZK48259.1"
                     /translation="MTTGRRKARREAVFALYQGDLMQRQRADVVGGSEAGAGAERDPY
                     QQRLIEGVLEHRQEIDAELAARLEGWTLERLAPLERNILRVGAYEVRWVIDTPAAVAV
                     AEAVALAKRYCSDEAGRLVNGVLGALVE"
     gene            1958..3868
                     /gene="dxs"
                     /locus_tag="VFD74_01490"
     CDS             1958..3868
                     /gene="dxs"
                     /locus_tag="VFD74_01490"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZK48260.1"
                     /translation="MNGANARLLDRLHLPEDLRTLDPVELRQLAAEVRAEIIDSISCV
                     GGHLGPSLGVVELTIALHAELRSPYDKIVWDVGHQSYAHKLLTGRADRFATIRTYGGL
                     SGFPKPSESEHDAFGTGHSSTSVSVGVGLAEAARLSADESDRRVFAVIGDGALTGGMA
                     YEAMNQAGHLRTPLVVVLNDNEMSIKKNVGAISSYLSRLRTEPTLYRWRRDLERRVQK
                     FPGIGERVYAMGEHVKEGVKAALVPGVLFEELGFQYIGVVDGHDIEALRLDIRRAMTL
                     DGPVLLHVRTIKGKGYSPAEKAPDRFHGISPFSIPTGECAPGSGGPPSYTEVFGKALV
                     ALARRDDRVVGITAAMAAGTGLDHLEQAFPERFFDVGIAEGHAVGFAAGLAAGGLRPV
                     VALYSTFLQRAYDQVIHDVCLPGLPVVFAIDRAGLVGEDGPTHHGAFDLSFLRAVPGL
                     TILVPKDEAELQSLLATALSMDSPVALRYPRGRGLGVPLRDHPIPIEGPWVEMLRTGG
                     GILLLGTGTGVALALAAAEALDAEGIPASVANVRKVHPLDDDELRPLLETHLGVVTVE
                     ENTIVGGFGSAVAELMQRTGIIRPMEFVGLPDEFVTHGDMRSLYGAIGFTVAGVQEAA
                     MRVLARSGAGAA"
     gene            3883..4692
                     /locus_tag="VFD74_01495"
     CDS             3883..4692
                     /locus_tag="VFD74_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HZK48261.1"
                     /translation="MRLDQILVDRGLVESRNAARGLIMAGLVKVEGLREDKAGAPVRT
                     DAAVDIQQRPRFVSRAGEKLAHALEALEVDPAGCSALDVGASTGGFVDCLLQSGASRV
                     IALDVGHGQLDARFRADPRVSVIERTNARHLTPEQLPFQPDLLTVDVSFISLEKIMPA
                     VISCMAALFTGLLLVKPQFEAGPRNVGKGGIVRDTAVHVEVLRRVGVALQALGLTVAG
                     LCDSGLPGTGGNVEFVYRVDRGRGDGLSPATLDRLVCHVARAEGGSEWATA"
     gene            4677..>4863
                     /locus_tag="VFD74_01500"
     CDS             4677..>4863
                     /locus_tag="VFD74_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48262.1"
                     /translation="MGNRVKCVALFTHQVADVSAEMLAVALPILLENGVRLLLPPGEA
                     LKHPDLRRTCSDGCREVG"
BASE COUNT          794 a         1486 c         1713 g          870 t
ORIGIN      
        1 tgcatcgaca acgacctggt gttcgtgggg cccacgccgg agagtatcga caagctgggg
       61 gacaagtccc tggccaagcg catcatggcc gaagccggtc tgcctacgat ccccgggacg
      121 gaggggatcc tggagagtgt cgccgaggcg acccgggtgg ctgctgacgt aggctatccg
      181 gtgatgctca aggccgcttc gggaggcgga ggccgtggga tgagattggt tggatccccg
      241 gctgcgctgg agcgctctta tcaggcggcc tcggccgagg cgctggccgc cttcggcgac
      301 gggggtctgt atctcgagaa ggtcgtcgag cggccccacc atgtggaggt gcaggtgatc
      361 ggagacggtc gaggaggcgt cttgacactg ggggagcgcg actgttccat ccagcggcgc
      421 caccagaaag tactcgagga gtctccgtct ccgcttctgg atgccgaggg ccgagcgcgt
      481 ctcttggagg tagcgactga ggcgtgttcc gcaatacgct acctcagtgc gggtactctc
      541 gagttcctgg ccgatgagca tagagacttc ttcttcatgg agatgaacac aagggtgcaa
      601 gtggagcatc cagtcaccga gatggtcacg ggcatagacg tggtgcggga gcagatccgc
      661 gtggcttcgg gggagcccct gatcgcgaca ggaagcgtgg tgccgaacgg ccacgccatg
      721 gagttccgaa tcaatgcgga agacccggcg aacggattct tgcctcgcgg aggcgtcatc
      781 aagcgcctgc ggctgcccgg aggtcccggg gtccgtgtag acactcacct gtacgaagga
      841 tacgaggtcc cgacgaacta cgactcacta ctggccaagg tcattgtgtg ggacagggat
      901 cgcgcctcgt gcatcgcccg gggattgcgc tgtctcgacg aattggagat cgagggtcta
      961 cccacgacga aggaccttca cctcgacatc ctgcggcacg agcggttcgt cgccggcaac
     1021 atctccaccg cctatctcga cgaggcgcgc gaagaacttg cgacgttgag gggcgcgacc
     1081 ccatgttgag aggagagtcg atgctgtcga tggagggatc ggagctgatt gtcgccgact
     1141 accggatcag ccccgcggtc ctccgagcga tcgttcgcca ttccctcggg ggagatccgc
     1201 gtgttcgtct cgcccatggg gtctcacggg cgggccggca cgcggtcgat gttgccgtga
     1261 gcgaaggggc gtgcgcggtg accgtgcaca tgcaggcgcc gcttggtgag gatctcctgg
     1321 cgctggggtc gaaggtcaag gaggatgtgg agacggggtt ggcggccatg acggggatgc
     1381 cagtagctcg agtggacgtc cacgtagacg gcgtatacag agcctccgcc tccacccaga
     1441 cggccacctc cgccgtcacc tccacctttg cccccgagtc ctgcgaccct attccggacg
     1501 cggcggcatg accacaggga gacgcaaggc gaggcgcgag gcggtgttcg cactgtacca
     1561 gggcgacctc atgcagaggc agcgcgcgga cgtcgtcggc ggttccgagg ctggtgcggg
     1621 tgcggagcgg gacccctacc agcagcggct catcgaaggc gtgctcgaac atcggcagga
     1681 gatcgatgct gagctagcgg cccgcctgga gggctggacg ctcgagcggc tcgcgccgct
     1741 cgagcgcaac atcctgcgcg tgggtgcgta cgaagtgagg tgggtgatcg acactcccgc
     1801 ggcggtcgcc gtcgcggagg cggtcgcgct cgcgaagcgt tactgttccg atgaggccgg
     1861 tcgcctggtc aacggggtcc tgggcgcctt ggtcgagtag tcccggacgg tttcggctgg
     1921 gaaccctcac gcatgcagag tatactcaga ggtcagtatg aacggcgcca acgctaggct
     1981 tctcgatcgt ctccaccttc ccgaagacct ccgcaccctt gacccggtcg agttgcggca
     2041 gctggccgct gaggtccgcg cggagatcat cgactccatc tcgtgtgtgg gcggccatct
     2101 ggggccgagc ctcggcgtcg tggagctcac catcgccttg cacgcggaac tgcgttcgcc
     2161 atacgacaag atcgtgtggg acgtaggaca tcagtcctac gcccacaagc tgctaaccgg
     2221 ccgcgccgat cgtttcgcca ccatccgcac ctatggcgga ctctccggct tccccaagcc
     2281 cagcgagagc gagcacgacg cattcggcac tggtcacagc agcacttcgg tgagtgtggg
     2341 cgttggattg gctgaagcgg cccgattgag cgcggatgag tccgaccgca gggtgttcgc
     2401 ggtcatcggg gatggcgccc tgaccggggg catggcatac gaggccatga atcaagccgg
     2461 acacctgagg acacccttgg tcgtcgtgct gaacgacaac gagatgtcga tcaagaagaa
     2521 cgtcggcgcc atcagttcct acctgtcgag acttcgcacg gaacccacgc tgtatcggtg
     2581 gcggcgcgat ctcgagcgcc gcgtgcagaa gttccctggg atcggggagc gcgtctacgc
     2641 tatgggtgag cacgtcaagg agggcgtcaa ggcggcgctg gtccccgggg tgctcttcga
     2701 ggagttggga ttccagtaca ttggcgttgt cgacggtcac gacatcgagg ctctgaggct
     2761 ggacatccgc cgtgccatga ccttggacgg gccggtgctc ctgcacgtgc gcaccatcaa
     2821 gggcaagggc tattctccgg cggagaaggc tccggaccga ttccacggga tatccccgtt
     2881 ctccatcccc acgggggagt gcgcgcccgg ttcgggaggg cctccctcct acactgaggt
     2941 cttcggcaag gcgctcgtgg ctctggcccg ccgggacgac cgcgtggtgg ggatcacggc
     3001 ggccatggcc gccggcacgg ggctcgatca cctcgagcag gcgtttccag agcgcttctt
     3061 cgacgtgggc atcgccgaag gtcatgctgt gggtttcgcc gccggactgg ctgcgggtgg
     3121 cctgcgacct gtggtggccc tgtattcgac cttcctgcag cgcgcctacg atcaggtgat
     3181 ccacgatgtg tgcctaccgg ggctcccggt ggtgttcgcc atagaccgcg ccggcctggt
     3241 gggggaagac ggacccacgc accacggtgc gttcgacctg tcgttcctgc gcgctgtgcc
     3301 gggcctcacc atcctggtgc ccaaggacga ggccgaactc cagagtctac tggccacggc
     3361 gctgtcgatg gacagccccg tggcgctgcg ctacccacgg gggcgaggcc tgggcgttcc
     3421 gttgcgcgat catccgatcc ctatcgaagg accctgggtg gagatgctgc ggacaggcgg
     3481 tggcatcctg cttcttggca caggcacggg ggtcgcgctg gctttggccg cggccgaagc
     3541 cctagacgca gagggaatcc ccgcgtccgt cgccaatgtc cgcaaggtcc atccgctcga
     3601 tgatgacgaa ctcaggcctc tgctcgagac ccacctaggg gtggtgacag tcgaggagaa
     3661 caccattgtc ggtggcttcg gctcggccgt ggcggaactc atgcagcgaa cgggcatcat
     3721 caggccgatg gagttcgtcg gccttccgga cgagttcgtg acgcatgggg atatgcgctc
     3781 gctgtacgga gccatcgggt tcacagtggc aggcgtccaa gaggccgcga tgcgggtgct
     3841 cgcccgttcg ggtgcggggg cggcttgagg ggagcagatc gagtgagact tgatcaaatc
     3901 ctcgtcgacc gtggattggt tgagagccgg aacgctgccc gggggctcat catggccggg
     3961 ctcgtgaagg tagaggggct ccgggaggac aaggcggggg ccccggtccg aaccgacgca
     4021 gcggtggata tccagcagcg cccccggttc gtgtctcggg cgggcgagaa actggcccac
     4081 gcgctcgagg cgctcgaggt tgacccggca gggtgttcgg cgctggatgt gggcgcatcg
     4141 acgggagggt ttgtcgattg cctgctccaa tccggagcgt cccgggtcat cgctctggac
     4201 gtgggtcatg gacagctgga tgcccgcttt cgggcggacc ctcgcgtcag tgtgatcgag
     4261 cggaccaacg cacggcacct gactccggag cagcttccct tccagcctga cctccttacg
     4321 gtggatgtat ccttcatatc cctcgagaag atcatgcccg ccgtcatctc ctgcatggcc
     4381 gctctgttca ccggacttct gttggtgaag cctcagttcg aagccggccc ccggaacgta
     4441 gggaagggcg gcatcgtccg ggacacggcc gtgcacgtgg aggtgctccg ccgggttggc
     4501 gtggccctgc aggcgctggg ccttaccgtg gcggggttgt gcgactccgg gcttccgggc
     4561 acgggtggca acgtcgagtt cgtctatcgc gtcgatcgag gtagaggaga cggcctttct
     4621 cctgctacgc ttgaccggtt ggtgtgccac gtagcccgag cggagggagg gtccgagtgg
     4681 gcaaccgcgt gaagtgcgtc gccttgttca cgcatcaagt cgccgacgta tccgccgaga
     4741 tgctcgcagt ggctctgccc atcctcttgg agaacggcgt caggcttctg cttcccccag
     4801 gcgaggccct caagcacccc gacctccggc ggacctgctc cgacgggtgc cgtgaggtcg
     4861 ggg
//
LOCUS       DASRMJ010000051         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_328909, whole genome shotgun sequence.
ACCESSION   DASRMJ010000051 DASRMJ010000000
VERSION     DASRMJ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328909"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFD74_01505"
     CDS             <1..447
                     /locus_tag="VFD74_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZK48263.1"
                     /translation="IITHLWEDGHRPYTAIAKDVGLSEASVRQRVARMIRDRIFRVTV
                     IASAVELGYVAAGLGLKVREGSLTEVAVRIAEFPEVDFVSVCTGSCDLNVGVNCEDHA
                     KLYEFLTDKIRAIPGIETTELHVHVHVVKNEFGVAPLPRTVERLPQ"
     gene            complement(441..1618)
                     /locus_tag="VFD74_01510"
     CDS             complement(join(441..1369,1369..1618))
                     /locus_tag="VFD74_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611749.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZK48264.1"
                     /translation="MGKTRPPYPQEFKRQIIALHRAGRTITELSEEFEPSDVTIRDWV
                     RQAEADDGLRLDVLTTDEREELRRLRRECRQLKIEREILGKSSRLVRAGDRTDTTTLF
                     RFVSENQVHDSETRYPVATMCRLLDVSTSGYYAWLTRGPSDRDLADEWLLAKIRSIHA
                     LSEETYGTPRIHMELREQGICVGRKRIARLLRQTGLHGVSRRRRYRTTQKDPAARPHE
                     DLVERNFTAPGPDRLWVSDITFVPTWAGFLFLAVVLDAWSRKIVGWAMGNHLRAELVT
                     DALDMAIYQRKPDGVICHSDQGSQYTSLAFGKRCKEAGVRPSMGSRGDALDNAMCESF
                     FATLECELLDRRRFKTHAEAKMALFQFIEGWYNPHRRHSGIDYLSPVEYERRHMVPAY
                     "
     gene            complement(1867..2907)
                     /locus_tag="VFD74_01515"
     CDS             complement(1867..2907)
                     /locus_tag="VFD74_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019075789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZK48265.1"
                     /translation="MPELAGRVDYVIGVDTHRDTHTAAAVDRVGGVLEGISVCTSQRG
                     SKELLDFAQATAPGRRCWAIEGTGIYGASLTRFLLARGEWVVEVERPRRPARRRGKSD
                     ELDAIAAAREALAGEHLAEPRSPGERAALQMLLTLRRHLVQSKVQAIGLFKTLLVTAP
                     DALRESLAPLSTAEQVGRAARLRERRTVEEDTSVVVLRSLARRILASSAEAEELRARI
                     GSLITALAPQLLARPGVGVLAGAQVFVVWSHPGRLRSEAAFASLAGVAPIPASSGQSR
                     RYRLNRGGDRQLNCALHTIVLTRLRCHPPTMAYAARRLAEGKSPREIRRCLKRYVARE
                     LFKLLEGSTQMS"
     gene            3344..>3645
                     /locus_tag="VFD74_01520"
     CDS             3344..>3645
                     /locus_tag="VFD74_01520"
                     /inference="COORDINATES: protein motif:HMM:NF026008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HZK48266.1"
                     /translation="MQGHIKKRATWEYVAELGPQPLQRCPSCRKRYWIKRERLDSCPV
                     CQGPLEERVARRQEMKAGFATKREAQEALTTVMASLSGGTYIEPSRMLTSEFLRGE"
BASE COUNT          676 a         1115 c         1178 g          676 t
ORIGIN      
        1 atcatcaccc acctttggga ggatggacac cgtccctaca ccgccatcgc gaaggacgtt
       61 ggcctctcgg aagccagtgt gcggcaacgc gtggctcgga tgatccgcga cagaatcttc
      121 cgggtgaccg tcatcgcgag cgcggtggaa ctcggatacg tcgccgccgg actcggtctc
      181 aaggtgcgcg aaggcagcct cacagaggtg gcggttcgaa ttgccgagtt cccggaggtg
      241 gacttcgtct cggtgtgcac cggctcgtgc gatcttaatg tcggagtcaa ttgtgaggat
      301 cacgccaagt tgtatgagtt cctcaccgac aagattcgcg ccataccggg gatcgagacg
      361 accgagttgc acgtgcacgt gcacgtggtg aagaacgagt ttggagttgc cccgcttccg
      421 cggacagttg agcgccttcc tcaataggcc gggaccatgt gcctcctttc gtactccacc
      481 ggagagagat agtcaatgcc ggagtgccgc cggtgggggt tgtaccaacc ctctatgaac
      541 tggaagaggg ccatcttggc ttcggcgtgg gtcttgaacc ggcggcgatc gaggagctcg
      601 cattcgagcg tcgcgaagaa gctctcacac atggcgttgt ccagggcatc ccctcgggat
      661 cccatggagg gacgcacccc cgcctctttg cagcgcttcc cgaaggcgag ggaggtgtat
      721 tgactcccct ggtctgaatg gcagatcaca ccgtctggtt tgcgctggta gatcgccata
      781 tcgagagcgt ccgtgacgag ttccgccctc aggtggttcc ccatcgccca gcccacgatc
      841 ttgcggctcc aggcgtcaag gaccacggcc aggaagagga agccggccca ggtggggacg
      901 aaggtgatat ccgataccca caaacggtcg gggcccgggg cggtgaaatt gcgctccacc
      961 agatcctcgt ggggccgagc ggcggggtcc ttctgggtgg ttctatatcg gcggcgccgg
     1021 gagacgccgt gaaggccggt ctggcgcagc agtcgggcga tgcgcttcct gcccacgcag
     1081 ataccctgct cgcgtagctc catgtggatg cggggagttc cgtaggtctc ctcggagagt
     1141 gcgtgaatgc tgcggatctt ggcgaggagc cactcgtcgg ctaagtcgcg atctgacggt
     1201 cctcgggtga gccacgcgta gtacccgctg gtggagacgt ccagcaggcg gcacatggtg
     1261 gctacggggt agcgtgtctc ggagtcgtgc acctgattct cgctcacgaa ccggaagagc
     1321 gtggtggtat ctgttcggtc tcccgcgcga accaggcggc tgcttttgcc aggatctcac
     1381 gctcgatctt cagctggcga cactcccggc gcaggcgacg gagctcttca cgctcgtccg
     1441 tcgtcagtac gtctaagcgc agaccgtcat cggcctccgc ctgtcggacc cagtccctga
     1501 tcgtgacatc cgacggctcg aactcctctg agagttcggt gatggtacgg ccggcacggt
     1561 gaagcgcaat gatctgtcgc ttgaactcct gtggataggg tggtcgagtc tttcccatct
     1621 cttggacacc tccttgaaac aaaggatacc gggtgtccac tgaagcgggt taactccagg
     1681 aagcaatagc aagccaaaga agctccatcc cggtcctacc cgccaaagtg aggagtgtga
     1741 agtgtcggtc cccaggatag cagcccagga ccttgcctct gccacgttgg cggtgtttgt
     1801 ccgaccccct gttgcgatca tcaggcatga gagtgggggt cccgacccca gacgcttcta
     1861 tgtttgtcaa gacatctggg tactcccctc aagcagctta aagagttctc gggccacgta
     1921 cctcttaagg caacgcctga tctctcgggg agacttgcct tcggccaggc gacgagcggc
     1981 atatgccatg gttgggggat ggcacctgag gcgggtgagg acgatggtgt gaagagcaca
     2041 gttaagctga cgatcgccgc cccgattcag gcggtatcgc ctggactgcc cggaagaggc
     2101 ggggatgggg gcaactccgg ccagcgaagc aaaggccgcc tcagagcgaa gacgtcccgg
     2161 gtgtgaccag accacgaaga cctgggcgcc ggcaagaacg ccgacaccgg gtcgagccaa
     2221 gagctgggga gcgagggcgg taatgaggct tccgatgcgt gcccttagct cctccgcctc
     2281 ggcactcgag gcgagtatgc ggcgggcgag cgacctaagg acgacgacgc tcgtgtcttc
     2341 ttccacggtt cgccgttccc gcagacgggc cgctcggcct acctgctcgg cagtggacag
     2401 gggcgcaagg gattcgcgca gggcgtcagg cgcggtgaca aggagcgtct taaagaggcc
     2461 gatcgcctgc actttgcttt gcaccagatg acgccgcagg gtgaggagca tctgcaacgc
     2521 ggcccgttca cccggggatc tcggttcggc caagtgttca ccggccaaag cctcgcgagc
     2581 cgcagcgatg gcatcgagct catcggactt gccccgacgc cgcgccggtc gtctcgggcg
     2641 ctcgacctcg accacccatt cgccccgggc aagaagaaag cgggtgaggc tggcgccgta
     2701 gatgccggtt ccctcaatgg cccagcagcg cctgcccggt gctgttgcct gggcaaagtc
     2761 gaggagttcc ttagatccgc gctggcttgt gcagacgctg atgccctcaa gaactccccc
     2821 cacccgatca acggcggcgg cggtgtgagt atcgcggtgg gtgtccacgc cgattacgta
     2881 gtcgactcgt cccgccagtt ccggcattcg actctccttt ttttgcggtc tatgcttgga
     2941 gacgtgccga cctggcaccc gttcgggcaa gactgtgatg ggtcacgcca gatggggcgg
     3001 acaagcttct atcaggcaac cgatgacggg accgggtcgg caccggcaga aacaggcaga
     3061 caagtctggg gcagggcacc agtagagggc cggtccactt ttgagtcatg ctcattcctg
     3121 tcgggccaac ctatcagcca ggcggcttca ggtacctacc cctctacact ttgtccacat
     3181 tggggaaggc ccaggaggga gtatcacagt ccactttatc cactttcggg gtccggggat
     3241 tcgcgccccc cggacttgcc atttccggcg atacgagcgg taatggacct cccgtcagtc
     3301 gggtgcgtgg acgagcgagt gagcggcgaa ggcagagcaa cacgtgcagg gtcacataaa
     3361 gaaacgcgcc acctgggaat acgtagccga actcggcccc caacccctgc agcgttgtcc
     3421 ctcctgccgc aaacgctact ggatcaaacg ggaacgcctc gactcctgcc ccgtctgcca
     3481 gggccccctg gaggagcggg tggcacgtcg ccaggagatg aaggccggat tcgccaccaa
     3541 gcgcgaagcc caggaagcct tgaccacggt catggcctct ctctccggcg gcacctacat
     3601 cgagccctcc cgcatgctga cgagtgagtt cctccgggga gagtg
//
LOCUS       DASRMJ010000052         6365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_474496, whole genome shotgun sequence.
ACCESSION   DASRMJ010000052 DASRMJ010000000
VERSION     DASRMJ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474496"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFD74_01525"
     CDS             complement(<1..366)
                     /locus_tag="VFD74_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZK48267.1"
                     /translation="MLRELNEQQRAAVLHGDEPLLIIAGAGTGKTTTLANRVAYQIAG
                     GVNPARILLLTFTRRAAAEMLRRADGALRRAAAAPTAGEAGRAGEASCGPASAATLAV
                     LPPHAGARVWGGTFHSTAAR"
     gene            817..2973
                     /locus_tag="VFD74_01530"
     CDS             817..2973
                     /locus_tag="VFD74_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZK48268.1"
                     /translation="MMDRIARFIVAHNKRVVAATVLLSLLAATMLFRMSFNADVTSFI
                     IEGNATGREYFALQQKYDTGDPINALISVPDGESFAQGAVLARLLLLSQEIEKLEGVK
                     AVATVIPESNPLTGQAITPELLATLPDAVVEQGLLTTPMAELLISEDHRDTMLMILPE
                     GDGYDLAGRLQDWLAANAPSDFEVTLTGNPVVFETVFGMLTWFLLAIPPVVVLLLLGV
                     FYANIGDRKLTIMAILPAVLGSVWTFGFIFGLGRKIDIVTILVPIFVIVMGSADGLHF
                     VTHYQEEAARTKDKVDRVAATLRQVGVPMILTTISTAAGFLSLLVADVRPIQQLGLFA
                     AVGISFAGVISFFFLPALLSHVHVEPKAHKAILGRRVTAGIKALVLRRWVGVVLAIGL
                     VAFAAIFIPRLQVNSDQLFMFKKDHQIRQDFARMQDALGGATPLIGEFTFDPNADPAS
                     ELRRVLAVSAELEALPGVRRVFSIADVAGLLAQRAPEQAAAALRGDLVTPLGDMVSAD
                     GLRFVLFPGDFTTADLRGWLDFAAHSDDIRVITGVPVLWDEMARLVVRAQIGSLGMAF
                     LLVAIMLFVAYRRVRETLAGLAPLVLTIGALLGFIAASGIQLNLVTAIVSSIVIGVGI
                     DYSIHFIAAIDYARAGGPGYVLRAIDSAGRPIVANALGIALGLTGLWLSPFAIHGKIS
                     MIMWVSMTTAALSALLVIPLFLPREGIVRPDEGYRP"
     gene            3067..3861
                     /gene="heR"
                     /locus_tag="VFD74_01535"
     CDS             3067..3861
                     /gene="heR"
                     /locus_tag="VFD74_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HZK48269.1"
                     /translation="MNATAAVKGSPADAATEARLLNLRWWNIIVGLILAAQAVVIAVL
                     ANGFSLPVVATYMAGPPGTEPTLTKLFDLPLGWGVFAFMAISAAALLIIASPGVFEWY
                     KGNLLKGRNYGRWIEYSISSSIMIVLIAMICGITDIAAIMAVFGVNASMIFFGLLMEK
                     YESPGKPSWLSFWFGSFAGIVPWLAVLVYVWSPRLDGPAPPTFVYAIIVSLFLFFNVF
                     AINMVLQYKKIGPWRDYLFGEKVYIVLSLTAKALLAWQVFSATLVG"
     gene            complement(3924..4886)
                     /locus_tag="VFD74_01540"
     CDS             complement(3924..4886)
                     /locus_tag="VFD74_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZK48270.1"
                     /translation="MQVHLHRHQGVGAAIAFTIEPYPQFSWSMTRQRLLENCPRSYYF
                     RYYLSHNGWLDDAPERSRLAYRLGKLTSLDALFGQEMDERAREIERRVRAGLSPPSLV
                     ELEEATRRTLRAAWKSSRDERTAFERSPKRTVMLRSFYLDDSPPDQREIERINEKIPT
                     CTANLLAAADWEVVAACGGDGCVLIPDFAHFYLGGVKVFAAPDLVYVAEGRLHVIDWK
                     SGKPGDDDHLQVLLSAYCVGLENPGLSLSPVLHYLLSGESVRPDVPDDLETFVADTVS
                     PGLRAMRDLLHDPLENAPLPESEFPRRESGLCSYCNFSRLCEAG"
     gene            complement(4853..5452)
                     /locus_tag="VFD74_01545"
     CDS             complement(4853..5452)
                     /locus_tag="VFD74_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48271.1"
                     /translation="MATVPNFLGKHMELPEDRLYSPGAHFWFGTAPAGAAGGDAGGGS
                     GADHAPGGTAAADMGADAYTAFEVGLTEPGIALTGGLVDLEVLVETGAAIVSGEEVAF
                     ATTRKAIKYFLSPVAGRVTAVNSAADADAANGSPYSTWLFRMELSPGADAGLVDAPAY
                     AAKLAASEHATEAAVLAAAAAKAGKASPTCRSIYTGIKE"
     gene            complement(5992..>6365)
                     /locus_tag="VFD74_01550"
     CDS             complement(5992..>6365)
                     /locus_tag="VFD74_01550"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZK48272.1"
                     /translation="VTTMMTGLGGLGVLGGLGGMFGLGGLLMLGMGLIPLLLIGLVIW
                     VVVQATRRRDDSSHTVSPAMSQAQWAPPSGAKPSTGMPPATGPGGVGPAPSSALAILE
                     ERYARGEMDRDDFLARKQDLR"
BASE COUNT          947 a         2091 c         2159 g         1168 t
ORIGIN      
        1 ccgagcggcg gtggagtgga aggtgccccc ccacacgcgg gcgcccgcgt gtggggggag
       61 gaccgcgagg gtcgcggcgc tcgcggggcc gcagctcgcc tcgcccgctc gaccggcttc
      121 gcccgccgtt ggcgccgcgg cggcgcggcg cagggcgccg tccgcccggc ggagcatctc
      181 tgccgcagcc ctgcgcgtga acgtgagcag caggatacgc gcgggattga ccccgccggc
      241 gatctggtag gccacacggt tggcgagcgt ggtggtcttc ccggtgccgg ctccggcgat
      301 gatgagcagc ggctcgtcgc cgtgcaggac ggctgcccgc tgctgttcgt tgagttcgcg
      361 gagtatggcc tcgcgcgcgg gcgacgcggg gtcgccgcgg ccggtcgagg cggcgtcggc
      421 tcgggagggc gacgcgacgt ccctccggcc gcccgcactc gggagccgcg cgatcgccga
      481 tgcctccgcc tccgtcagtc gcgacgccgt cggcacgtct gcgacggcgg tgatgccgtt
      541 cgccacaagt cctcccttgg gtagctcccc aatcgacagc ctacaggcag gctcggacag
      601 aaccgcgcga cgccgccctc gcgcggacgt tggcggtcgc gaagatgccg gcggtcgcgc
      661 tatgatgagc gggcgtcgtc gcgggcgcat cggaggcgtg cgcccggccg tcgttgggat
      721 cagggctgcg tctcgcactg acaccgccgt cagcgccgcc gccgccgccg tcgccgctag
      781 tggcatcgca tccccgagca cgaagagctg gagggcatga tggatcggat cgcgcgtttc
      841 atcgtcgcgc acaacaagcg ggtcgtcgcg gccactgttc tcctctctct gctggccgcc
      901 accatgctct tccgcatgtc gttcaatgcc gacgtgacct cgttcatcat cgagggcaac
      961 gccaccggcc gcgagtactt cgcgctccag cagaaatacg ataccggcga cccgatcaac
     1021 gcgctcatct ccgtgcccga cggcgagtcg ttcgcgcagg gcgccgtcct ggcacgcctg
     1081 ctcttgctct ctcaagagat cgagaagctg gagggcgtca aggcggtggc caccgtcatc
     1141 ccggaatcca atccgctcac gggccaggcc atcaccccgg aacttctggc gacacttccc
     1201 gatgccgtcg tggagcaggg tctgttgacc acgccgatgg ccgaactact catctcggaa
     1261 gatcatcgcg acaccatgct gatgatcctt cccgagggcg acgggtacga cctcgcgggg
     1321 cggctccaag actggctcgc cgccaacgct cccagcgact tcgaggtgac cctcaccggc
     1381 aaccccgtgg tgttcgagac cgtcttcggc atgctcacct ggttcctgct ggccatcccc
     1441 ccagtggttg tgttactgct tctgggggtg ttctacgcca acatcggcga ccgcaagctc
     1501 accatcatgg ccatcttgcc ggcggtcctg gggtcggtgt ggaccttcgg cttcatcttc
     1561 gggctcggcc ggaagatcga catcgtcacc atcttggtgc ccatcttcgt gatcgtgatg
     1621 gggtcggcgg acggactcca cttcgtgacg cactatcagg aggaggcggc gcgcacgaag
     1681 gacaaggtgg atcgcgtcgc ggcgaccctc cgacaggtgg gagtccccat gatcctcacc
     1741 accatctcca ccgcggcggg cttcctgtcg ctgctggtgg ccgacgtgcg tcccatccaa
     1801 caactcggcc tgttcgccgc cgtgggcatc tccttcgcgg gcgtcatcag tttcttcttc
     1861 ctccccgcgt tgctcagcca cgtgcacgtg gagcccaaag cgcacaaagc cattcttgga
     1921 cgccgggtca ctgctgggat caaagccctc gtgttgaggc ggtgggtggg tgtcgtcctc
     1981 gcgattggcc tggtggcctt cgccgccatc ttcatccccc gccttcaggt gaacagcgac
     2041 cagctcttca tgttcaagaa ggatcatcag atccgccagg acttcgcccg catgcaggac
     2101 gcgctgggcg gagcgacacc cttgatcggg gagttcacct tcgatccgaa tgcagacccg
     2161 gcctccgagc tgcggcgggt cttggcggtc agcgccgaac tggaagccct accaggggtg
     2221 cggcgcgtgt tcagcatcgc cgacgtggcc ggactgctgg ctcagcgcgc tcccgagcag
     2281 gctgcggcgg cgcttcgcgg cgacctggtc actcccttgg gggacatggt cagcgccgac
     2341 ggcctgcggt tcgtgctgtt ccccggcgat ttcaccaccg ccgacctgcg aggctggctc
     2401 gacttcgcgg ctcacagtga cgacatccgg gtcatcacgg gagtacccgt gctttgggat
     2461 gagatggcgc gtctggtggt gcgcgctcag atcggttcgc tcggcatggc cttcctgctg
     2521 gtggcgatca tgctgttcgt ggcctatcgt cgcgtgcgtg agaccttggc cggtcttgcg
     2581 cccctggtgc tgaccatcgg cgcccttctc ggcttcatag cggcgagcgg tatccaactc
     2641 aacctcgtga cggccatcgt ctcgagcatc gtcatcgggg tgggcatcga ctactccatc
     2701 cacttcatcg ccgccatcga ctacgcgcgg gcgggcggac ccggctacgt gcttcgggcg
     2761 atcgactctg ccggccgccc catcgtcgcc aacgccctgg gtatcgcctt ggggctcacg
     2821 ggcctgtggc tctcgccttt cgcgatccac ggcaagatct ccatgataat gtgggtgtcg
     2881 atgaccaccg ctgctctcag cgcgctgctc gtgatccctc tctttctgcc gcgcgaaggc
     2941 atcgtccgac ccgatgaggg gtacagaccc taggttgccg agcgtcgttc cggtgggatg
     3001 gcctcagatg cggacggtct cggctcctag gtgttcttcc gttggtcatc gtgggaggta
     3061 tatcgaatga acgcaaccgc ggctgtcaaa ggatcacccg ctgacgctgc gaccgaagcc
     3121 cgtctgctca acttgcgctg gtggaacatc atcgtgggat tgattctggc cgcgcaggcg
     3181 gtggtgatcg ccgtgctggc caatggcttc tctctgccgg tggtcgccac ctacatggcc
     3241 ggtcctcccg gcacggagcc gacgctcacc aagctcttcg atctgcccct gggctgggga
     3301 gtcttcgcct tcatggcgat ctcggcggcg gcgctcctga taatcgcgtc gcccggcgtc
     3361 ttcgagtggt acaagggcaa cctactgaag ggtcgtaact acgggcgctg gatcgagtac
     3421 tcgatcagtt cttcgataat gatcgtcctg atcgccatga tctgcggtat caccgacatc
     3481 gcagccatca tggcggtgtt cggggtcaac gcttcgatga tcttcttcgg gttactcatg
     3541 gagaagtatg agagccccgg aaagccgagt tggctctcgt tctggttcgg ttcgttcgcg
     3601 gggatcgtgc cctggctggc ggtgctggtg tacgtgtgga gtccgaggct cgacggcccg
     3661 gcgcctccga cgttcgtgta cgccatcatc gtgtcgctgt tcctgttctt caacgtcttc
     3721 gcgatcaaca tggtgttgca gtacaagaag atcggcccct ggcgcgacta tctctttggg
     3781 gagaaggtct acatcgtgct cagtctgacg gcgaaggcgc tgctggcctg gcaggtgttc
     3841 tcggccaccc tggtggggta gaggaggccg acccggcacg ggcggcctgc acgatcggcg
     3901 ggcgggtgcg ccggatcggc gcctcagccc gcctcacaca gtcgcgagaa attgcagtag
     3961 gagcagagcc ccgattcccg ccgcgggaac tcgctctcgg gcaggggcgc gttctcgagg
     4021 ggatcgtgca ggaggtcgcg catggccctc aggcctgggc tcacggtgtc ggcgacgaag
     4081 gtctcgaggt cgtcggggac gtcgggtctt acgctctcgc ccgagagcag gtagtggagc
     4141 acagggctca ggctgagacc cgggttctcg aggcccacgc agtaggccga cagcagcacc
     4201 tgcaggtggt cgtcatcgcc gggcttgccg ctcttccagt cgatgacgtg cagccggccc
     4261 tcagcgacgt acaccaggtc gggtgcggca aagactttca ctccgccgag gtagaagtgg
     4321 gcgaagtcgg ggatgagcac acagccgtcg ccgccgcatg cggccacgac ctcccagtcg
     4381 gccgcggcca gaaggttcgc ggtgcacgtg gggatcttct cgttgatgcg ctcgatctcg
     4441 cgctggtcgg gcggggagtc gtcgagatag aaggacctca acatcaccgt ccgtttgggt
     4501 gagcgctcga aggcggtgcg ctcgtcgcgt gacgacttcc aggcggcgcg cagggtgcgg
     4561 cgcgtggctt cctccagctc cacgaggctc ggtggcgaga gaccggccct cacgcgccgc
     4621 tcgatctctc gggcgcgctc gtccatctcc tgaccgaaga gggcgtcgag cgacgtgagc
     4681 ttgcccagac ggtaggccaa cctgctgcgc tcgggggcgt cgtcgagcca gccgttgtgc
     4741 gagaggtagt agcggaagta gtaggagcgg ggacagttct caagcagtcg ttggcgcgtc
     4801 atggaccacg agaattgagg atatggctcg atggtgaagg cgatggcggc gcctactcct
     4861 tgatgccggt gtagatggac ctgcacgtgg gggaggcctt acccgccttg gccgccgctg
     4921 ccgccaagac tgcagcctcg gtggcgtgtt ctgacgccgc caacttggcg gcatacgcgg
     4981 gggcgtcgac caggccggca tccgcgccgg gcgacagctc catgcggaag agccaggttg
     5041 aatagggcga cccgttcgcc gcgtcggcat cggcggcgct gttgacggcc gtcaccctgc
     5101 cggccaccgg gctcaagaag tacttgatgg ccttgcgcgt ggtcgcgaag gccacctcct
     5161 caccggagac gatcgccgcg ccggtctcca ccagcacctc gaggtcgacg aggccgccgg
     5221 tgagcgcgat gcccggctcg gtgaggccga cttcgaacgc ggtgtaggcg tcggcgccca
     5281 tgtccgcggc ggccgtcccg ccgggcgcgt gatccgcgcc gctgcctccg cccgcgtcac
     5341 cgcccgcggc gcccgctggg gcggtgccga accagaaatg ggcgccgggg ctgtataggc
     5401 ggtcttccgg gagttccatg tgtttgccca ggaagttcgg tacggtcgcc atcgtctact
     5461 ccccttgtgt tgtcggcgtg atcgtccgga tcggcagcgt cgcgagtcgc gtgcgcaggt
     5521 cgcaggcgtt cggatcgagg gtgccaacgg aagtatatgg gtattcccgc gcgccgggaa
     5581 gtcgatgccg gggagtcggc gtcttcaagg catgcgcact tcgccgtttg cccggacgat
     5641 cttgcccggg atgttcgcga aggcggggga cagacacaca tcaccccggg tgcggggaga
     5701 cgtcggaggc gcccgaccac gccgacctcg cgggcacccg tccaaggcgt tgatggagtt
     5761 cggaagtctg actgcgctca atcgtctatg caatcaggga gttgcgtttc gtgatttgca
     5821 gacttaggcg cgtccggagt tgtcccggct tgcggaatcg cagcggtgcg tccacgccgg
     5881 cgcctcgggc aacaaacagc cgcgccgctt tgtgtgtgta ggcggcgcgg ctgagtgtag
     5941 gaggagagct tcttcgggag tcgtcgcagt ggcgccgctc cggagaggtc gttaccgaag
     6001 gtcttgcttg cgggcgagga agtcgtcccg gtccatctcg ccgcgcgcgt atctctcttc
     6061 gaggatcgcc agggcggacg agggagccgg cccgaccccg ccggggcccg tggcgggcgg
     6121 catcccggtg gacggtttcg cgccggaagg cggcgcccac tgcgcctgcg acattgcggg
     6181 cgacaccgtg tgggaactgt cgtcgcgcct gcgggtcgcc tgcacgacca cccagatgac
     6241 caaaccgatc agcaggagcg ggatcaaccc catacccaac atcagaagac ctccgagtcc
     6301 gaacatccca ccaagaccac caagaacacc aagaccgccg agaccggtca tcatcgtcgt
     6361 cactc
//
LOCUS       DASRMJ010000053         5798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_148138, whole genome shotgun sequence.
ACCESSION   DASRMJ010000053 DASRMJ010000000
VERSION     DASRMJ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148138"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFD74_01555"
     CDS             complement(<1..858)
                     /locus_tag="VFD74_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HZK48273.1"
                     /translation="MSLTNRQTEILRLVTERYIRSGQPVSSREVAQGVGIRVSPSTVR
                     SEFGVLEEQGYLTHPHTSAGRLPTHRGYRSFVDGLMAVVGGRDSGVAPLDFHVLEKEV
                     DVALRQTSEAMAEATNLLALVVAPRTSGATVRHIELLLLQPRLVMVVFIVSTGRVAKW
                     VVDYPDLVDAGMVEWARTYLNESLSAHMITDRLVRRTLDNPELGKKEMRFLHTLSPAF
                     ERLLDEQAGEGLYVGGASRLLSDQPTQDFENLRELLLLLEERYQLLRALRSVLATGRV
                     VVRIGDEHPH"
     gene            complement(969..2144)
                     /gene="hemW"
                     /locus_tag="VFD74_01560"
     CDS             complement(969..2144)
                     /gene="hemW"
                     /locus_tag="VFD74_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZK48274.1"
                     /translation="MVTGDHLGLHAAASAAADASHQVTHLYVHLPFCGTRCAYCDFAS
                     EAIGPHRRSGALAAYMDKMGREAALLDPLLARPLETVYVGGGTPTAVPHHLLAALLVD
                     LGRLVGPSTEFTVEANPETLDDATLELLASHRVTRLSLGVQSFLPTGLAALGRRTPAG
                     ATEKALDAMRRAGWAEWSLDLVFGIPGQDLAALEHDLGAAVAQRPPHISVYDLTYTDR
                     FARRLESQQGPDGRAAAEEFADAHYALVSDRLSVAGYRRYEVSNYALSGHESRHNLGY
                     WRGEDYVGLGASAVSTVGGTRWTNPRSVVAYVAGEPAEVELLSPRVRLFERAMLGLRT
                     AEGVTERGVEDVVDAAELKRFVGLGLVERRCATLRLSPRGLDLGNAVLAAVLRSPEE"
     gene            complement(2152..2343)
                     /locus_tag="VFD74_01565"
     CDS             complement(2152..2343)
                     /locus_tag="VFD74_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48275.1"
                     /translation="MTVEYLIVGAVLIVGGVVQRRMRRYLPPEQQAGGGVAGRWSGVL
                     GYVAVGLGVVLLVAGVLGR"
     gene            complement(2343..4151)
                     /gene="lepA"
                     /locus_tag="VFD74_01570"
     CDS             complement(2343..4151)
                     /gene="lepA"
                     /locus_tag="VFD74_01570"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZK48276.1"
                     /translation="MTGFEHIRNFSIIAHIDHGKSTLADRILQLTHTVAEREMREQML
                     DRMDIERERGITIKAQAVRVYYRAQDGLDYQLNLIDTPGHVDFSYEVSRSLAACEGAI
                     LVVDASQGIEAQTLANTYLALENDLEIIPVLNKIDLPGAEPELRAQEIVALIGCTAAD
                     ILHISAKTGQGVAEVMEAVVRRVPPPKGDSDGPARALIFDSFYDQYRGVVAFVRVVDG
                     TFRRNDVIRTFATQRKSEVEEVGIFSPDMLKTDLLRAGEVGYIITGVKEVAHLRPGDT
                     LTHADRPAATPLPGYREAVPMVFCGLFPIEGNDYPELKDALEKLTLNDASLHWEPETS
                     RALGFGFRCGFLGLLHMEIVRERLQREFGLELIATTPNVKYDVELTSGVVLAISNPVD
                     FPDAGLIVEVREPYISASVLTPNEFVGSVMELCQDKRGTFRDMEYLSADRVRLVYDLP
                     LAEIVLDFFDLLKTRTKGYASLDYEMKDFESGAMVRLDILIAGEPVDALSLIVHKDRA
                     YPVGRALVERLRKQIPRQMYEVAIQAAVGSRVVARETVRAMRKDVLAKCYGGDITRKR
                     KLLEKQKAGKKRMKMVGSIEIPQEAFLAVLDLDEKR"
     gene            complement(4333..>5798)
                     /gene="murJ"
                     /locus_tag="VFD74_01575"
     CDS             complement(4333..>5798)
                     /gene="murJ"
                     /locus_tag="VFD74_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZK48277.1"
                     /translation="VPSLIRTLVADTALSAAFIPVFSGLLSEERRKEAWQLVTTLTFL
                     AVVLLGAISALGMVFAPQVISVAAPGYRDPATIDLAIGLTRVMFPTVVLLGVTGIFMG
                     VLNSYDHFTLPALAPIVWNVIIIASVVFFAGNSGDDRSFFILAWGTVIGTVAQLVIQM
                     PAVWRRRESLGLRLDFRDPAVRQVGRLLGPVVLTLGIVNFNALVGTIVASYISEPAPA
                     VIDKAFRLFQLPQGMFAVAIGTVLFPSLSRFATAGRMGDFRVALSSGIRQIFFVTLPF
                     SAFFVVLGAPTVRLVFEHGKFQAEDTAAVAWALAFFSLGMAFVSANTLLNRAFYGIRK
                     AWLPLVTGIANLVLYTGLCLLLYKPLGVGGVTLATAAVSTFNFFGLMYLLRRQIGSVD
                     GRRVFSSAARSVVSLAPLCLAAYGTWWFVDGLLGRSVPAQLVSVGAAYLLGLLAYAAA
                     ARVMRMDELGEVLSVVRRRRDASVTGAVIRQESEDLP"
BASE COUNT         1072 a         1980 c         1876 g          870 t
ORIGIN      
        1 gtgcggatgt tcgtcgccga tgcgcaccac cacgcgcccc gtggcgagca cggagcgcag
       61 agcgcgcaat agctggtagc gctcctccag cagcaggagc agctcgcgga gattctcgaa
      121 atcctgcgtc ggttggtcgg agagcaaccg cgacgccccg cccacgtaca gaccctcgcc
      181 ggcctgctcg tcgagcaggc gctcgaacgc gggagaaagc gtgtgaagga agcgcatctc
      241 cttcttacca agctcgggat tgtcgagcgt gcgacgcacc agccgatcgg tgatcatgtg
      301 ggcactcagc gactcgttca agtaggtgcg cgcccactcc accatgcctg cgtccaccag
      361 gtcgggatag tccacgaccc acttggccac acgccccgtt gacacgatga acacgaccat
      421 gaccaggcga ggttggagca gcagcagctc gatgtgcctg accgtggccc cgctggtgcg
      481 gggagccacc accaacgcca ggaggttggt ggcttcggcc atcgcttcgc tggtttggcg
      541 aagcgcgacg tccacctcct tctcgagcac gtggaagtcg agcggcgcga cgccggaatc
      601 tctcccgccg accacagcca tgagcccgtc gacgaacgaa cgatagcctc tatgggtggg
      661 cagcctgccc gcggatgtgt gggggtgagt gaggtacccc tgctcttcaa gaacgccgaa
      721 ctcgctacgc accgtggacg gcgagacgcg gatgccgacg ccctgcgcga cctcgcgcga
      781 agacacgggt tgaccggagc ggatgtagcg ctcggtgacg agtctcagga tctcggtctg
      841 tctgttagtg aggctcattc gacttcccac gcagaccatc cgcctgccac ggcgcacagc
      901 ctacgaccct catcgtggac gctgtccgac ccgcgacgcg ctgttgtgga tctgcccggc
      961 tccggagatc actcctccgg cgagcgcagg acggccgcca gaacggcgtt gccgagatcc
     1021 aggcctcgcg ggctcagccg aagtgtagca cagcgcctct cgaccagacc caggccgacg
     1081 aagcgcttca gctcggccgc atcgaccacg tcctcgacgc cccgctcggt cacaccttcc
     1141 gccgtacgaa ggccgagcat ggcgcgttcg aacaggcgga cccgtggcga aaggagctcc
     1201 acctcggccg gttcccccgc cacgtaagcg accaccgacc tcgggttggt ccatcgggtg
     1261 ccgccaaccg tgctgacggc cgaagcccca agacccacgt agtcctcgcc ccgccagtat
     1321 ccgagattat gccgcgactc gtgcccggac agggcgtagt tggacacctc gtagcgacgg
     1381 tagcccgcca cggagagccg atccgacact agggcgtagt gggcatccgc gaactcctcc
     1441 gccgccgccc gcccgtccgg accctgctgc gactcgagac gacgtgcgaa gcggtcggtg
     1501 taggtgaggt cgtacacgga gatgtgcggc ggtcgctgcg cgaccgccgc acccaggtcg
     1561 tgctccaacg ccgcgagatc ctgtccggga atgccgaaca ccaggtcgag actccactcc
     1621 gcccacccgg cgcggcgcat cgcgtcgagg gccttctcgg tcgcccccgc gggcgtccgg
     1681 cgccccaggg cggccaggcc ggtgggcagg aaactctgca cgcccaagga gaggcgggtg
     1741 acccggtgcg aggcgagcag ttccagggtg gcgtcatcga gggtctcggg attagcttcc
     1801 acggtgaact cggtgctcgg gccgacgagc cgccccaggt cgacgaggag ggcggcgagc
     1861 aggtggtgag gcacggcagt gggcgtccca cccccgacgt agaccgtctc gagcggccgg
     1921 gccaggagag gatcgagcag cgccgcctct cggcccatct tgtccatgta ggccgcgagc
     1981 gcgcccgagc gccggtgcgg gccgatggcc tctgaagcga agtcacagta cgcgcagcgg
     2041 gtgccacaga aaggaagatg cacgtagagg tgggtgacct gatgcgacgc atcggccgcg
     2101 gctgatgccg cggcgtgcag accaaggtga tcgccggtca ccatgccgag gctaccggcc
     2161 caggactcct gccaccaaca gcaccacacc gagccccacc gcgacgtagc ccaggactcc
     2221 gctccacctg cccgccacgc cgccgccggc ctgctgctca ggaggcagat agcgcctcat
     2281 cctccgctgc accacgcccc caacgatcag gaccgcgccc acgatgaggt actcgacggt
     2341 catcagcgtt tctcgtcgag atcgagcacg gcgaggaagg cctcttgcgg tatctcgatg
     2401 ctgcccacca tcttcatgcg cttcttgccc gccttctgct tctcgagcaa cttgcgcttg
     2461 cgcgtgatgt cgccgccgta gcacttggcc aacacgtcct tgcgcatggc ccgcaccgtc
     2521 tctcgggcaa ccactcgact gcccaccgcc gcctggatgg cgacttcgta catctgccgc
     2581 ggtatctgct tgcgcagtct ctcgaccagg gcccgaccca ctggataggc tcggtccttg
     2641 tgcacgatga ggctgagcgc gtcgaccggc tctcctgcga tgaggatgtc gagacgcacc
     2701 atcgcgccgg actcgaagtc cttcatctcg tagtcgagag acgcgtatcc cttcgtgcgg
     2761 gtcttgagca ggtcgaagaa gtcgagcacg atctcggcca gcggaaggtc gtacaccagc
     2821 ctcacccggt cggccgacag gtattccata tcgcgaaagg tgcctcgctt atcctggcac
     2881 aactccatga cgctgccgac gaactcgttg ggggtgagca ccgaagccga tatgtacggt
     2941 tcgcgcacct cgacgatgag tccggcatcg gggaaatcca ccggattgct gattgccagc
     3001 acgacgccgg aagtcaactc cacgtcatac ttcacgttcg gcgtggtggc gatcagctcc
     3061 agtccgaact cccgttgcag gcgctcacgc acgatctcca tgtgcagcag acctaggaag
     3121 ccgcagcgga acccgaaccc gagggctcgg gaagtctccg gttcccaatg cagcgacgcg
     3181 tcgttcaggg tgagcttctc gagggcgtcc ttcaactcgg ggtagtcgtt gccctcgatg
     3241 gggaagagcc cgcagaagac catgggcacg gcttcacggt agccgggcag aggcgtcgcg
     3301 gccggacgat ccgcgtgtgt caaagtgtcg cccgggcgca ggtgcgccac ttccttgacg
     3361 cccgtgatga tgtaccccac ctccccggcc cgcagcaggt cggtcttcag catgtcggga
     3421 ctgaagatgc ccacctcctc cacctcgctc ttgcgttgag tggcgaaggt gcggatgacg
     3481 tcgttgcgcc ggaaggtgcc gtcgaccacg cggacgaacg ccacaacgcc gcgatactgg
     3541 tcgtagaacg agtcgaagat gagcgcgcgg gccgggccgt cgctgtcgcc cttcgggggt
     3601 ggcacgcgac gcaccacagc ctccatgacc tcggcgacgc cctggcccgt cttggccgag
     3661 atgtgcagga tgtcggcggc cgtgcaaccg atgagcgcca cgatctcctg cgcccgcaac
     3721 tccggctccg ccccgggcag gtcgatcttg ttgagcaccg ggatgatctc gaggtcgttc
     3781 tccagggcca gataggtgtt ggccaacgtc tgggcctcga tgccctggct cgcatcgacc
     3841 accaggatgg cgccttcgca ggccgccagg ctgcgcgata cctcgtagct gaaatcgaca
     3901 tggcccggag tgtcgatgag gttcaactgg tagtcgagac cgtcttgggc ccggtagtag
     3961 acccgcaccg cctgggcttt gatggtgatg ccgcgttcac gctcgatatc catgcggtcg
     4021 agcatctgct cgcgcatctc tcgctcggcc accgtgtgcg tgagttggag gatgcgatcg
     4081 gccagggtgg acttcccgtg gtcgatgtgg gcgatgatgg agaagttgcg tatgtgctcg
     4141 aaaccggtca tggcgaggta ttgtacccca acgggaagag acctccgggc actccgccgg
     4201 cccggcaaga ccacccggca cgatcaccat gttcgcgccg atgcaccgcc ggcgcgcccc
     4261 gaaccggcgc gccccgaacc gacgcacccc gggtcccgcg cgagcgaacc gtcccgaaga
     4321 acggcaccgc ccctacggca gatcctcact ctcctgcctg atgaccgctc ccgtgaccga
     4381 ggcgtcgcgc ctgcggcgca cgacgctcag cacttcgccc aattcgtcca tgcgcatgac
     4441 gcgcgccgcc gccgcatacg cgaggaggcc gagcagatac gcggcgccca ccgacaccag
     4501 ttgggccggc accgaccgtc cgagcaggcc gtccacgaac caccaggtgc cgtatgcggc
     4561 caggcagaga ggcgccagcg aaaccaccga ccgcgccgcc gacgaaaaga cccgccgtcc
     4621 gtcgaccgac ccgatctgcc gacgaagcag atacatgagc ccgaagaagt tgaacgtaga
     4681 gaccgccgcg gtggcgaggg tcacgccccc tacgccgagc ggcttgtaca agagcagaca
     4741 gagccccgtg tagagcacga ggttcgcgat ccccgtgacc agtggcagcc acgccttgcg
     4801 gatcccgtag aacgcccgat tgagcagtgt gttcgcgcta acgaaggcca tccccaggct
     4861 gaagaaggcc agagcccagg ccacggctgc ggtgtcctcc gcctggaact tgccgtgctc
     4921 gaacacgagg cgcacggtag gcgcccccag caccacgaag aacgcagaga agggcagcgt
     4981 gacgaagaag atctgccgaa taccgctgga aagagccacg cggaagtcgc ccatccggcc
     5041 tgcggtcgcg aaacgcgaga gcgacgggaa cagcacggtg ccgatggcca cggcgaacat
     5101 gccctggggc aactggaaca gcctgaaggc cttgtcgatg accgcggggg cgggctcgct
     5161 gatatagctg gccacgatcg tccccaccag cgcgttgaag ttgacgatgc ccagcgtcag
     5221 cacgacgggc ccgagaagcc gaccgacctg ccgcaccgcc gggtcgcgaa agtcgaggcg
     5281 cagaccgaga gactcccggc gccgccacac cgcgggcatc tggatgacca gctgcgccac
     5341 ggtgccgatc accgttcccc aggccaggat gaagaaggag cggtcatccc cgctgttccc
     5401 ggcgaagaag accaccgacg cgatgatgat cacattccac acgatggggg cgagcgcggg
     5461 cagtgtgaag tggtcgtacg agttgagcac gcccatgaag atcccggtga ctccgagcag
     5521 caccacagtg gggaacatga cccgagtgag acctatggcc aggtcgatgg tcgcaggatc
     5581 gcggtatccg ggagcggcga cgctgatgac ctggggagcg aagaccatac ccagagcgct
     5641 gatcgccccc agaagcacga cggcgaggaa ggtcaaggtg gtgaccagct gccaggcttc
     5701 tttgcgacgc tcctcggaga gcaggccgga gaacacgggg atgaaggccg cactcagcgc
     5761 cgtatccgcc acgagtgtgc ggatcaggct gggcacct
//
LOCUS       DASRMJ010000054         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_115253, whole genome shotgun sequence.
ACCESSION   DASRMJ010000054 DASRMJ010000000
VERSION     DASRMJ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115253"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFD74_01580"
     CDS             <1..606
                     /locus_tag="VFD74_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZK48278.1"
                     /translation="SSESLGERSMGPSARGADVTALQKVLQRRGLLDREPTGVFDAAT
                     AEAVVAFKVARGSPATPVLDAESLLGLLGSIVIDRSERKLYLYDGGQLVLTYPVAVGQ
                     PRYPTPTGKFRIVSKVYHPSWTPPPSPWAEGLEPVPAGPDNPLGTRWMGLSARHVGIH
                     GTYQSGSIGTAASHGCIRMRIRDVEDLFERVFVGTPVEIIP"
     gene            603..1028
                     /locus_tag="VFD74_01585"
     CDS             603..1028
                     /locus_tag="VFD74_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48279.1"
                     /translation="MTVGPTASAPPRRRPARVSGGRGFTPGRRSTGKMLRDALLVIVI
                     LAMGLPFVVARVGGAPLTVDQLDAAKAGRRAAYEQQDWLANSMSNKVARVAVESGGTR
                     FEFRYYTFFGLPWGSSEALVASDGSVSDVSTSLTLGRVF"
     gene            1130..1732
                     /locus_tag="VFD74_01590"
     CDS             1130..1732
                     /locus_tag="VFD74_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZK48280.1"
                     /translation="MLSIRHALLGLLARGDQHGYELKTAAEAELVPLSSLNFGQIYTA
                     LDKLERDGYLSHRTVEQEERPAKKVFALTESGRAELASWMRSASVPDLDLRNPTFFKL
                     LLARGLDPAVGCPDVLEVIRGERRACFERLHEVVAARARASGGEQPLGASLLLDLAAL
                     RLEAFLSWLDRCEAALAADGGGSAGGLGGGNYTEEAWTQR"
     gene            1717..>2510
                     /locus_tag="VFD74_01595"
     CDS             1717..>2510
                     /locus_tag="VFD74_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48281.1"
                     /translation="MDTAVETLGVRKEFGSGETRVEALRGVDLRLDQGEFVAVMGPSG
                     SGKSTLLHLIGGLDRPTGGVVRVGGQDLAVLDDDALTLVRRRRIGFVFQFFNLLPILT
                     AEENVALPLVIDGIDDKESHRRSLAAMEAVGIAHRASHRPAELSGGEQQRVAIARALV
                     SEPVLILADEPTGNLDSVSGDQVIALLRRLADEQGQTILMVTHDAKDAAVADRVVFLK
                     DGLVDDEQRLPDAPPADEVLRGLEAAAAAAAGSAAAASLASCVTIR"
BASE COUNT          381 a          824 c          897 g          408 t
ORIGIN      
        1 tcctcggaga gtctcgggga acgttcgatg ggaccctcgg cccgaggggc cgacgtgacc
       61 gcgctgcaga aggtcttgca gcgacgcggc ctcctggacc gggagcccac cggggtcttc
      121 gatgccgcca ccgccgaggc cgtcgtggcc ttcaaggtgg cgcgcgggtc gcccgccacg
      181 ccggtgctcg acgcggagag tctccttggg ctcctgggct ccatcgtcat cgaccgctcg
      241 gagcgcaagc tctacctgta cgacggcggc cagcttgtgc tgacgtaccc ggtggccgtg
      301 ggccagccgc gatatcccac gcccacgggc aagttccgca ttgtcagcaa ggtctaccac
      361 cccagttgga ctcctccgcc ctcgccgtgg gccgagggtc tcgagccggt cccggcgggc
      421 cctgacaacc ctctcggcac ccggtggatg ggactgtcgg cgcgtcacgt gggcatccac
      481 ggaacctacc agagtgggtc catcgggacc gccgcttcgc acgggtgcat ccgcatgcgc
      541 atccgcgacg tggaggacct cttcgaacgg gtgttcgtgg gaaccccggt ggagatcatc
      601 ccgtgactgt gggccccaca gcgtccgcgc cgccgcgcag gcggccggcg cgcgtctcag
      661 gcggccgcgg cttcactcca ggacgacgca gcacgggcaa gatgctgcgg gacgcgcttc
      721 tggtgatcgt gatcctggcc atgggcctgc cctttgtcgt ggcccgggtc ggcggcgcgc
      781 cgctcaccgt cgaccaactg gatgcggcga aagccggtcg tcgcgccgcc tacgagcaac
      841 aggactggct ggcgaactcc atgagcaaca aggtcgcccg cgtcgccgtg gagagcgggg
      901 ggacccgctt cgaattccgc tactacacct tcttcggcct tccctggggg agttcggaag
      961 cgttggtggc ttccgacggc tcggtgagcg acgtgtccac cagtctcact cttggcaggg
     1021 tcttctagcc gccgacttcg gcccccggtc cgtgccgtgt ccttgcgccc ggtccgcgca
     1081 ggtgatatat acaaaggata taggctaaga atgagagatt ctagaggcga tgctgtccat
     1141 cagacacgcc ttgctagggc ttctcgcgcg cggcgatcag cacggatacg aactcaagac
     1201 ggctgcggaa gctgaactcg tgccgctgtc gtcgctcaac ttcgggcaga tctacacggc
     1261 gctcgacaag ctcgagcggg atggctacct atcgcaccgg acggtggagc aggaagagcg
     1321 tccggcaaag aaggtgttcg ccctcacgga gtcgggccgc gccgagttgg cgtcgtggat
     1381 gcgctcggca tccgtccccg atctcgatct gcgcaacccg accttcttca agctcctgct
     1441 ggctcgcggg ctggacccgg ccgtggggtg ccccgacgtc ctcgaggtga tcagggggga
     1501 gaggcgcgcc tgcttcgagc ggctgcacga ggtggtcgcg gcgcgggcgc gcgcgtcggg
     1561 cggggagcaa ccgttgggcg catcgctcct gctcgacctg gccgcgctgc ggctggaggc
     1621 gttcttgagt tggctggacc ggtgtgaggc ggcgctggcc gcggacggcg gcggaagcgc
     1681 cggcggcctc ggaggcggaa actacacgga ggaagcatgg acacagcggt agagaccttg
     1741 ggagtgcgca aagagttcgg tagcggagag acgcgggtcg aagcgctgcg cggggtcgat
     1801 ctgaggcttg atcagggtga gtttgtcgcg gtcatggggc cgtcggggtc gggaaagagc
     1861 accctcctgc acctgatcgg cggtctggac cggccgaccg gaggcgtcgt ccgcgtgggc
     1921 ggccaggatc tcgccgtcct cgacgacgac gccctcactc tggtgaggcg ccggcgcatc
     1981 ggcttcgtgt tccagttctt caacctgctg ccgatcctca cggccgagga gaacgtggcg
     2041 ctgcccctcg tgatcgacgg catcgacgac aaggagtcgc accgtcgcag tctcgcggcg
     2101 atggaggcgg tggggatcgc ccacagagcg tcgcatcgcc ccgcggaact ctcgggggga
     2161 gaacagcaac gcgttgcgat cgcgcgggct ctcgtctcgg agccggtcct gatactggcc
     2221 gacgaaccca cgggcaacct cgacagtgtc tccggcgacc aggtgatcgc cctgctgcga
     2281 cggctcgccg acgaacaagg tcagacgatc ctgatggtta cgcacgacgc caaggacgcg
     2341 gcggtcgcgg accgggtggt gttcctgaag gacgggctcg tggacgacga gcagcgcctg
     2401 cccgacgcgc ctcctgcgga cgaggtcctg agaggtctgg aggccgccgc cgcagccgca
     2461 gccgggtccg cggccgccgc gtccttggcg tcgtgcgtaa ccatcaggat
//
LOCUS       DASRMJ010000055         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_439916, whole genome shotgun sequence.
ACCESSION   DASRMJ010000055 DASRMJ010000000
VERSION     DASRMJ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439916"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFD74_01600"
     CDS             <1..207
                     /locus_tag="VFD74_01600"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZK48282.1"
                     /translation="LNYAVDYGFVDPAEVVAFLHEKPARLSVIITGRRARQEIMDEAD
                     TVTEMRSVKHPFRSGTRAQKGIEY"
     gene            204..890
                     /locus_tag="VFD74_01605"
     CDS             204..890
                     /locus_tag="VFD74_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK48283.1"
                     /translation="MSETRKNHQIPKLEAVKAHPQVAQFLSHADSYLAALGYTEHGTR
                     HASLVAHIAHNVLERLGYPDDTSQLAAVAGYLHDLGNFITRESHAQSAALLAFQILRD
                     LDYGPADCATVMGAIGNHDELLGRVIDPVSAALILADKSDVHRSRVRDPKTADTDIHD
                     RVNYAAERSFLNVDPVTREITLELTIDTGISGVMEYFEIFLSRMIMCRRAAEHLGCSF
                     QMAINELRIF"
     gene            913..1758
                     /locus_tag="VFD74_01610"
     CDS             913..1758
                     /locus_tag="VFD74_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZK48284.1"
                     /translation="MGVRMVPTNRARVVLERLVELNRISGDVATKSHAEVDEEADGDA
                     GLALTPVRRRRLYEDVVQQLQGLISSGRLEPGDQLPAERALAEQLAVSRASVREGLRT
                     LEARGMLEARPGQGLFVRGRRTEEIVSILASYLVRERESFLEVLDVREALERKAAERA
                     AILATTKDLEAIKAALVEMQEEVLEGRLPLESDTAFHRALGTASRNEVLSQTLNTVLG
                     LMSQRRQELLNSDYGGLLMLHQHVNIFRAVKNRDPKAASELISAHLRELAAPHRPRSG
                     YPRPS"
     regulatory      1961..2103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2193..>3910
                     /locus_tag="VFD74_01615"
     CDS             2193..>3910
                     /locus_tag="VFD74_01615"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZK48285.1"
                     /translation="MSENKMHTVIFQPSGSRGKVEDGKSILQASRELGVEIEAICGEK
                     RTCGKCKVRIEDGFFERYGVESSRDHVDPWRPVEAKFFSEERKEQGFRLACLCKIHGD
                     LVVFVPEEARAGGQVVRKAVTDRKINVNPSVRTYYIEMTPPTLHDPLGDYERVCDALK
                     AQHGLEPPILDYRVLLGMPDTLRAADWKVSVSVWSGGPVPEIVRIAPGTVTRSIGLAV
                     DVGTTSVAAYLCDLATGEVISYDSMMNPQVQYGEDVMSRITYAMSHDDGIDKLQAAII
                     EGVNTLAARVCEAVGFTHEDILELAIVFNTAMHHCFLGIYPEHLGLAPYAPALHHSIN
                     IKARDLGLKFCPGANVFVLPNEAGFVGADNVGVLIAEEPYNKHDRQLIIDIGTNGELI
                     MGNSEKLISSSCATGPALEGAHIHFGMRAAPGAIEKIYINPETKEPRYRVIGKDEWSD
                     EMEPETIGTKGICGSGIIDGVAEMFKTGIIDKTGKFNKDQNHPRIRKGEQGDEFVLAW
                     ANETSVGRDVTMTIKDVRAVQLAKGAMYAGAKIMMQKLGYDALDRVVLAGAFGSFIDR
                     EESLVIG"
BASE COUNT          754 a         1196 c         1287 g          673 t
ORIGIN      
        1 ctcaactacg ctgtggatta cgggttcgtc gatcccgccg aggtggtcgc gtttctccat
       61 gaaaaaccgg cgcggctctc agtgatcatc accgggcgcc gagcgcgcca ggagataatg
      121 gacgaagcgg acacagtgac cgagatgcgc tcggtcaaac acccgttccg cagcggcacg
      181 cgagctcaga aaggcatcga gtattgagcg agacccggaa gaaccaccag atccccaagc
      241 tggaggccgt caaggcgcat ccccaggtgg ctcagttcct gtcgcacgcg gacagctacc
      301 tcgcggccct cgggtacacg gagcacggaa cgcggcacgc ttccctggtc gcccacatcg
      361 cccacaacgt gctcgagcgc ctagggtacc ccgacgacac ctcacagctg gccgccgttg
      421 caggctatct gcacgatctg ggcaacttca tcactcgcga gagtcacgcg cagtcggcgg
      481 cgctgctcgc gttccagatc ttgcgcgatc tcgactatgg gcccgccgac tgcgcgaccg
      541 tcatgggggc catcggcaac catgacgaac tgctcggcag ggtcatcgac ccggtgtcgg
      601 cggcgctgat cctcgcggac aagtcagacg tgcatcgtag ccgggtgcgt gatcccaaga
      661 ccgccgacac cgacatacac gaccgcgtca actacgccgc ggagcgctcc ttcctcaacg
      721 tcgatccggt gaccagggag atcacgctcg aactcactat cgataccggc atctcggggg
      781 tgatggagta cttcgagata ttcctgtcga ggatgatcat gtgccggcgg gcagccgagc
      841 acctggggtg ttcgtttcag atggcgatta acgagctgag gatcttctga tgccgcgttg
      901 cggagtgtga tcatgggcgt tcgtatggta ccgacgaaca gggcacgcgt ggtgctggag
      961 cggttggtcg aactcaaccg catcagcggg gacgtagcga ccaagtcgca cgcggaagtt
     1021 gatgaggagg cggacggcga cgccggcttg gctctcaccc cggtgcgtcg ccgccgtctc
     1081 tacgaagacg tggtgcagca attgcagggt ctgatctcct cggggagact ggagcccggc
     1141 gatcaactgc ccgccgagcg ggcactcgcc gagcaactgg cggtgagtcg ggcgagtgtg
     1201 cgagaggggc tgcgcactct cgaggcgcgg ggtatgctgg aggcccgacc gggtcagggg
     1261 ctcttcgttc ggggcaggcg caccgaggag atcgtgagca tcctggcttc gtacttggtg
     1321 cgcgaacggg agagcttcct cgaggtgctc gatgtgcgcg aggccctgga gcggaaggcc
     1381 gccgagcgcg cagccatcct cgccacgacc aaggacctgg aagcgatcaa ggcggccctg
     1441 gtcgagatgc aggaagaggt gcttgagggg cggttgccgc tggaatcgga cacggctttc
     1501 catcgggctt tgggcactgc gtctcggaac gaagtgctga gccagacgct caacacggtg
     1561 ctggggctga tgtcgcagcg caggcaggag ctgctgaaca gtgactacgg tggccttctg
     1621 atgctgcatc agcacgtgaa catcttccgc gcggtgaaga acagagatcc gaaagccgca
     1681 tccgaactga tctccgcgca cctgcgcgag ttggcggctc ctcacagacc ccgctcgggg
     1741 tatccgcgcc ctagctgacc cctcgcagcg caggcgatca gcccgcgcga gatccgtctt
     1801 cgtcgcggac ggtctccgcc cgcttcctca tatcgttcct aagatgttga caagtttgcg
     1861 ctgtctgtga cagactaggg ctgttcagtg gtctgaccac tggacaacgc aaccagtaag
     1921 cggtcgaagc agtcgatgtt ggtcggggta gagatccgat ccgggtacgc cgtctcccct
     1981 gcggcacccg gaggcattca gaggtaagcc ggtcgaagtc cggcgcggtc ccgccactgt
     2041 agtcacccgg tcctcgaggc gcgggtgaga gccagaaaac tccaggggaa ctgtttggca
     2101 gagcttctcg aggcaagaag caagttgctt gccttgggga gctcttcgtt gttctcgagg
     2161 cggatcgaag tagcgagcgt gaaggagggt tcttgagcga gaacaagatg cacacggtga
     2221 tcttccagcc gtcgggtagt cgggggaagg tcgaggacgg caagtccatc ctgcaggcga
     2281 gccgcgaact tggggtcgag atcgaggcca tctgtggcga gaagcgcacg tgcggcaagt
     2341 gcaaggtgcg catcgaagac ggcttcttcg agcgctacgg agtggagtcc tcgcgggacc
     2401 acgtcgatcc gtggcgtccg gtcgaggcga agttcttctc cgaggagcgc aaggagcagg
     2461 gcttccgcct cgcgtgccta tgcaagatcc acggggatct cgtggtcttc gtgcccgaag
     2521 aggcccgcgc cggcggccaa gtcgtgcgca aagcggtcac cgaccgcaag atcaacgtca
     2581 atccctcggt gcgcacgtac tacatcgaga tgacgccacc caccctgcac gacccgctgg
     2641 gcgactatga gcgggtctgc gacgcactca aggcccagca cgggctcgaa ccccccatcc
     2701 tcgactatcg ggtcctgttg gggatgcccg acactctgcg cgccgcggac tggaaggtgt
     2761 ccgtgtcggt gtggtccggg ggaccggtgc ccgagatcgt gcgcatcgcc cccgggaccg
     2821 tgacgagaag catcggtttg gccgtggatg tgggcacgac gagcgtggcg gcctacctct
     2881 gtgacctcgc caccggtgag gtgatcagct acgactctat gatgaacccg caggtgcagt
     2941 acggcgagga cgtcatgagc cgcatcacct atgcgatgtc gcatgacgat ggcatcgaca
     3001 agctgcaggc cgccatcatc gagggcgtca acacgctggc cgccagggtg tgcgaggccg
     3061 tcggcttcac gcacgaggac atcctcgaac tcgccatcgt gttcaatacg gccatgcatc
     3121 actgtttctt gggcatctac cccgaacacc tcgggctcgc gccgtatgct ccggcgctgc
     3181 accactccat caacatcaag gcccgcgacc tcggtttgaa gttctgcccg ggcgccaacg
     3241 tcttcgtgct ccccaatgag gcgggcttcg tgggcgccga caacgtgggc gttctgatcg
     3301 ccgaggaacc gtacaacaag cacgaccgcc agctgatcat cgatatcggc accaacggcg
     3361 agctcatcat gggcaacagc gagaagttga tcagctcgtc ctgcgccacc ggacccgccc
     3421 tcgagggcgc gcacatccat ttcgggatgc gggccgctcc gggcgccatc gagaagatct
     3481 acatcaaccc cgaaaccaag gagccgcgct accgggtcat cggaaaagat gagtggtcgg
     3541 acgagatgga gccggagacc atcggcacca agggtatctg cggctcgggc atcatcgacg
     3601 gcgtggccga gatgttcaag accggcatca tcgacaagac cggcaagttc aacaaggacc
     3661 agaaccaccc ccgcatccgc aaaggtgaac agggcgacga gtttgtcctg gcttgggcga
     3721 acgagacctc cgtcggccgt gacgtcacca tgacgatcaa ggatgtgcgg gcggttcagt
     3781 tggcaaaggg agccatgtac gccggcgcca agatcatgat gcagaagctc ggctacgatg
     3841 cgttggaccg tgtcgtgttg gccggggcgt tcggctcgtt catcgaccgt gaggagtcct
     3901 tggtcatcgg
//
LOCUS       DASRMJ010000056        16620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_365139, whole genome shotgun sequence.
ACCESSION   DASRMJ010000056 DASRMJ010000000
VERSION     DASRMJ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 16620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16620
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365139"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VFD74_01620"
     CDS             <1..979
                     /locus_tag="VFD74_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HZK48286.1"
                     /translation="FWTHSSRRVLTLEWQEGLTLNQLDLETLTLAERYRLAETISRTW
                     FKQILEDGFFHGDPHPANITYIDPDHIALLDFGIAGGLSDVDLEQGTLLFLDIMDRDI
                     EGVKRRLQRLGVKWSRDRDQAATAALELGFSRYFGASLADIDPAALIREAFDIIYGLH
                     LSLPTRFLVLEKSLLTVEGVVTQIYPDFNVFEVARPYARKLLLRRYRPEVVGARLGRS
                     LAAYGEVLRDYPFQLHDLLEEMRDGELEIKFVHTGLEAFSHKLDILMNRLVVALVAAS
                     LGISSSLVAVFVTTGPRVLGMSLWGIPGLVAALFFGVWLMWAIFRSGRM"
     gene            1095..3608
                     /gene="recJ"
                     /locus_tag="VFD74_01625"
     CDS             1095..3608
                     /gene="recJ"
                     /locus_tag="VFD74_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZK48287.1"
                     /translation="MDTFTWSLKPFSWNVAERIAKELDLPMMVAIVLARRGFRDSEQV
                     RAFLEISPAVPDPFLFSDMRAAVAVVNQAAASGRRIVIHGDYDVDGVSATALLIRGLA
                     DLGVRAEHYLPSRFVQGYGLSETGVREIAAGGEALLVTVDCGVNYPDEVALARELGMD
                     VVVTDHHQPGDTLPDCPLIHPVRGGYPHSDLCGVGIALKLLHALHIDRSSVPPDRIPE
                     RLLAHLDLVALGTVADMVPLRGENRHYVAEGVVRIAASGKPGLRALVTVANGEGRVDA
                     QTVGFRLAPRINAAGRLGDPEIPLRLLLTDDAAEAETLAQGLDALNRERQSVEAVVLE
                     EALVVVESLEQMPPILVVAGRDWHEGVLGIVASRLVERYHRPAVLLSLAGGRARGSAR
                     SIRAYDIMAGLTACARHLTVFGGHPQAAGLTLPEDMVAEFKSDLERHAAEALVDADLL
                     PTYHPDAVAAGVALNLETVDALSRLAPFGFGNPAVKLIALAARLEDARPTRKGDHLQG
                     TLVVDEVRTRGIGFGLAGKLPELMEDGLRAHAGIRLEASEWQGTSRAEVQLHSFYRVA
                     PDLGEGALGCSPICPFLDDLEAPPACPACAHPFSGAAFTDVMGRDLRDAPGRLSHTAE
                     ILSTGEDAAILGADVKRHLVRLSGELPLRELGVVGTHCVSRHCWRTRLASPRGDTLLF
                     ADWDAAERRPELLCARSHVIVVDPVYRASQSALLQQIADSGARVHLLYGDEERRATEE
                     ELRVRMHPRYWMVALYRALRTDTGCDDGLFAGVMGASWEKDSVMPTSEDLRRASQLLT
                     ELGHGSNGGGGATMEASMSPAYTAAVEAFEEAVRLCRKT"
     gene            3593..5833
                     /locus_tag="VFD74_01630"
     CDS             3593..5833
                     /locus_tag="VFD74_01630"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZK48288.1"
                     /translation="MSEDVTQTPPPDEAQLDLPHRELLDDLFRQIVRYNPTFDRDMIT
                     RAFTVAVRKHKDQRRASGEDYILHPVGTATICADLRLDSPTVTAALLHDVVEDTDVSV
                     AEVVAEFGEEVGLLVDGVTKLTQMSFRSVEEEQAENFRKMIIAMAKDIRVILIKLADR
                     LHNMRTLSYLGKEKQIQKAKETLEIYAPLAHRLGIESVRWELEDLAFATLHPRKYSEI
                     QHMVSQRRADREEYLEEARSYLKSELEKVKINAEILGRAKHFYSIYDKMTRKGKEFNE
                     IYDLTAMRVLVASVRECYAALGIIHSLWKPLPGRFKDYIAMPKFNMYQSLHTTVIGPQ
                     GKPLEIQIRTSEMHETAEYGIAAHWIYKDKDRSKEREKDRIDRLAWLRQMMEWQSDTK
                     DATEFMESLRIDLLQDEVYVFTPKGEVKSLAVGATPVDFAYAIHTDVGHRCVGAKANG
                     RIVPLTYRLQSGDIVEILTTKNTQGPSRDWLNFVQTSGARNKIRQWFKRERREDSEHL
                     GKEALLESFRKHGLPAQKLMGSDTLSQVMKDMNFQKREDFFVSIGAGKTSPQQVLTKV
                     MQHMGQGTGAVKEELPPISILPSPRRLPTSADIGVRVEGIDDVAIRMPECCRPVPGDE
                     IVGYISLGKGITIHRKDCPNVRALERHPERFAEVSWDQAVRAPFRVEIQVEAYDRSRL
                     LEDISRTLSESGVNILAAHIQTTDDNTVKDRFVFEVPGVEYLDSVLQRIRMIDTVYDA
                     YRVTPH"
     gene            6066..6701
                     /locus_tag="VFD74_01635"
     CDS             6066..6701
                     /locus_tag="VFD74_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK48289.1"
                     /translation="MRILTVPVGPLQANCYLVADDGGIALAIDPGGEPERLISALERE
                     GMRLTHILLTHGHFDHLAGAAGLAELTGARIACSEGTAPMLRNPDDYIPFPGFGGIPS
                     REPDVLLADGDIISVGQLEVETVTTPGHSPGDVTFAVDGALFCGDLLFYRSVGRTDLP
                     GGDFDMLLQSVRRLVDRFPGESRVYPGHMQPTSLRDEVRANPFLSGLSSRG"
     gene            6694..7974
                     /gene="hisS"
                     /locus_tag="VFD74_01640"
     CDS             6694..7974
                     /gene="hisS"
                     /locus_tag="VFD74_01640"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZK48290.1"
                     /translation="MADRPTAPKGTYDILPNQQATRRWVVARATEVFRRYGYGRLDTP
                     VFEETRLFARGVGESTDIVRKEMYTFTDLGGRSMTLRPEGTAPCARAYVEHGMHKLPQ
                     PVKLWYHCPMFRYESPQSGRYRQHHQFGIEAFGSDSPALDGEAIGVLAELYRDIGLAQ
                     TELRINSMGCRQCRPTYSEELRSFLTEHRGELCPECLERSRLNPMRTFDCKVPGCGEV
                     LQNAPRLTDFLCPDCAAHHEEVKKYLGLQGIDYVEDHRLVRGMDYYTRTTFEFQSAVL
                     GAQSGVGGGGRYDDLVETIGGPPTPGVGFGTGVERILLALARSRDDLPAEASPAAYVV
                     ALSDSTRAEAFRFAHEARRLGAAVDIDYAGRSVKGQMKQAGRSRARYAVIVGEDEVAE
                     SSVTLKDLGTGEERTLPRIDAMTLIVEADDKGGS"
     gene            7971..9725
                     /gene="aspS"
                     /locus_tag="VFD74_01645"
     CDS             7971..9725
                     /gene="aspS"
                     /locus_tag="VFD74_01645"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZK48291.1"
                     /translation="MTYRTHWCGEIGEQTVGRRVSVAGWVHRRRDHGGLVFIDLRDRS
                     ALVQLVFDPEQHPVAHAAAHDLRGEYVIAATGQVVARGADQVNPRLATGSVEIHVDEL
                     TVLAESKTPPFFPEDDVDVDETLRLKYRYIDLRRQRMQHNLMLRDRVVQSIRRYLGSD
                     GFIEIETPILTKSTPEGARDFLVPSRLRPGDFYALPQSPQLFKQLLMVAGFERYYQIA
                     RAFRDEDLRADRQLEHTQIDVEMSFASETEIQDLIEGMVWHAFSETLQVDLPRPFPRL
                     TYTESMLRFGSDKPDTRFGMEIVDVSDLVGDADFKVFADAVAGGGAVRGLRAEGAAGF
                     SRKDVDELTKDAAVYGAKGVAPIWVEEGGIRSPIAKFLGEEATAAVVARMHGRPGDLL
                     LFVADALAIVEPALGALRLRLADLLGVTREGWSFLWVVDPPMFELDAREGRLKAQHHP
                     FTKPRVSCPEDLAEQPLRLGTEAYDLVLNGVELGSGSIRISDPALQRAVFGALGLDDD
                     EIRAKFGFLVEAMDFGMPPHGGMALGLDRFVMLMAGETSIRDVIAFPKTQSGSCPLTE
                     APSSVSRQQLEELHIKVR"
     gene            9828..9980
                     /gene="ssrS"
                     /locus_tag="VFD74_01650"
     ncRNA           9828..9980
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFD74_01650"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10001..10528
                     /locus_tag="VFD74_01655"
     CDS             10001..10528
                     /locus_tag="VFD74_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48292.1"
                     /translation="MQRHRKAVYPQVSLVVLVIAVALVLAACGGGGGSGAEETSTTIP
                     PASPTTAGAAAPTKAFADLVGTTLSTTEDTPEDVGNSLDLHVPMVLAFFVTGGTDDAI
                     VLEQLDKLALRFSDVDFYTYDYKVPAAYGDLAGQLHVDYPPQVTFVDSNGVIRGVTSG
                     YADEGTLNQLVANIR"
     gene            10600..12102
                     /gene="mnmA"
                     /locus_tag="VFD74_01660"
     CDS             10600..12102
                     /gene="mnmA"
                     /locus_tag="VFD74_01660"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZK48293.1"
                     /translation="MTPPSDTPTSPRAYSEAVLAHLRTPAHARRLPFAHAEGRAGSRA
                     CGDLVHIQLQVEAGAIVDAGFQAFGCPATIATASEIVTQLPGLDLLEAAHLNHEDVAA
                     RLALAEHKRGCSEVAADALYGALEDWVRTPAAISRPGVQRQGAVLVAMSGGVDSSAAA
                     LILKQEGLTPVGVTFRLWSDPTCSSGGGCCSPETVLRARRVAHALGMAHVTVDLTGEF
                     YDVVVDDFVQEYAAGRTPNPCVVCNSRLRFSALAQIADRLGIDKIATGHYARLLGSPP
                     RLHRALDPEKDQAYVLSRVDPGLLERVVLPLGELTKPEVRRLARDAGLETHDAKESQE
                     ICFIPDDDYRRFLAERLGVAGGPIIDRQGRVLGEHRGTYRFTIGQRKGLGLAAPEPLY
                     VCAIHDDGAVVVGTRDDLLVGEVELRDVVAHSTAVPGKDLTIQLRSSGLVVPATVTVE
                     GRRSTVRLAEPVSGVATGQTGVVFAGDRVVLAGAIHETQPLRAPGSSRQG"
     gene            12210..12584
                     /locus_tag="VFD74_01665"
     CDS             12210..12584
                     /locus_tag="VFD74_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HZK48294.1"
                     /translation="MEWDAAAFMKIAIGLFFLAFAGGLTVMLVRLAVVFKEIAAMLAD
                     VTKEFLPILSRLQTTVDEVNSELGKIDEITGSVVSVTGAIESTTSAVQSAISAPFRKV
                     AGVSAGLSEALSSFLSGRRKEH"
     gene            12584..12832
                     /locus_tag="VFD74_01670"
     CDS             12584..12832
                     /locus_tag="VFD74_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48295.1"
                     /translation="MGKFAFGLTVATAAALGAAAALAHRISTETGKSFSEALNEVPAE
                     AERYLEEILARGREAYDAGRTAAAEKQAEITNRLGERL"
     gene            12861..13265
                     /locus_tag="VFD74_01675"
     CDS             12861..13265
                     /locus_tag="VFD74_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48296.1"
                     /translation="MGATTDTVTLTIPQREDLLPLFRMILGGIALRRNLSLETLDDLQ
                     LAVDTVLAEDEAQRGDVSMSVESEAGVLRIRLSPLTSRDLRETLLQGSVPEGAGDRCL
                     DVCLILRSLVDDFAIHDGTDDSFAVVLRKLTG"
     gene            13270..14091
                     /locus_tag="VFD74_01680"
     CDS             13270..14091
                     /locus_tag="VFD74_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HZK48297.1"
                     /translation="MVDGGTANDTARAGLFAVPLGVVAEKELLRRIHREADLAAREEL
                     ITRYLPLVKSLARRFASRGQPVEDLVQVGSIGLIKAIDRFDIERGVELSTYATPTILG
                     EIKRYFRDKGWAVKVPRALQDLNIRLNKVIEQLTVELNRSPTIAELAVACSVTEEDVL
                     EALESGRAYNSLSLFSSGGSDDEDSLELLDYLGKEESEYELVEQRRYLAPAMGRLDER
                     ERLILHLRFFEGMTQTQIASRVGISQMHVSRLIRKSIDMLRQTMREQGEESSPLD"
     gene            14153..>16620
                     /gene="alaS"
                     /locus_tag="VFD74_01685"
     CDS             14153..>16620
                     /gene="alaS"
                     /locus_tag="VFD74_01685"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZK48298.1"
                     /translation="MKTRDLRKLFLDYFVENGHLLMPSASLVPYRDPSVLLTTAGMQQ
                     FKPYFLGMAEAPATRMTSAQKCFRTTDIDRVGFTARHCTFFEMLGNFSVGDYFKEGAI
                     RFGYELSTERLGLDPARIWVSVFEGDAQVEADEEAMRQWEQVGIPRERMVALPRSENF
                     WGPPGPTGPCGPCSELYYDRGSDRGCGSPDCRPGCDCDRFLEYWNLVFMQYNMDDQGR
                     LTPLPSRNIDTGMGLERIAALMQGVDSVFLTDSFYPLIQLGEEISGLAYGGSERADAA
                     LRVLADHSRSMSFLIADGVLPGNEGRGYVLRRIIRRAARFSRNLEMTPPFLGRFAERT
                     IELLADAYPELHERRETIMRIVNSEEERFNRTLDQGLVLLDEEIRRARGGGTKMFSGA
                     AAFVLHDTFGFPVEVTREIVEEKGLSLDLDGFDAAMEGQRARARATSKELDSLHEAAV
                     HLARESQNATEFVGYAQDEVFTVIDSLQEFEDGDVVLTLRESPFYAEGGGQTADIGWI
                     ETDAGRAEVRDVQHHGAVQVIVAHTTAGSLEQGVRAKAVISSAHRHSVAANHTGTHLL
                     HHALRSTLGKDVTQAGSSVRADKFRFDFAFHEPLGSDRLTEIEEIVNRKIVENHPVRA
                     FTTSLDHARDLGATALFGEKYGDFVRVVEIDDFSRELCGGTHVHSTSEIGIFKILSEG
                     SVGANVRRIEAVTGRRAVEYYRSQDSTVSAAAGMLAVQEDALLPAVNKLQTRVAGLTR
                     EVGQLLAAKAGDLTSAVVEAAIEADGVKVAVAQVDARDNDHLLALLDQVRDRLAPAIV
                     LLATVSAGKVTSAAAVSNGVDGSDA"
BASE COUNT         3002 a         5382 c         5316 g         2920 t
ORIGIN      
        1 cttctggacc cactccagca ggcgtgtgct cacactcgag tggcaagagg gcctcacact
       61 caaccaactg gaccttgaga cgctgaccct cgccgagcgc taccgtctgg ccgagaccat
      121 ctctcggacc tggttcaagc agatcctcga ggatggcttc ttccatggtg acccgcaccc
      181 ggcgaacatc acctacatcg atcccgacca catagctctg ctcgacttcg gcatcgcggg
      241 tgggctctcc gatgtggatc tcgagcaggg cacgctgctg ttcctcgaca tcatggatcg
      301 tgacatcgag ggcgtgaagc gacggctgca gaggctggga gtgaagtggt cccgtgacag
      361 ggaccaagca gcgacggcgg cactcgaact cggcttctcc cgctacttcg gagcctccct
      421 ggccgacatc gacccggcgg ccctgatccg agaagcgttc gacatcatct acggtctgca
      481 cctgagtctg cccacccgct tcttggtgct ggagaagtcg ctcttgaccg tggagggagt
      541 cgtcacccag atctaccctg acttcaacgt gttcgaagtg gctcggccat atgcgcgcaa
      601 gctcctcttg cgtaggtatc gtccagaagt cgtcggcgcc cgtctcggac ggtctctcgc
      661 ggcctacggc gaagtgcttc gcgactaccc gttccaactt cacgaccttc tcgaagagat
      721 gcgcgatggg gaactcgaga tcaagttcgt ccacacgggc ctcgaggcgt tcagccacaa
      781 gcttgacatc ctgatgaatc ggctggtggt cgccctggtg gccgcgtcgc tcggcatctc
      841 cagctctttg gtcgcggtgt tcgtcactac ggggccgcgc gtcctgggca tgtcgctgtg
      901 ggggatcccg ggcttggtcg ccgccctgtt cttcggagtc tggctcatgt gggccatatt
      961 ccgctctggg cgtatgtagc tcaccgtcca gagacgtgcg gggaatggcg gcggccgccc
     1021 gaggtatggc agcagcccgc gaaacggtgc aggaccggcg atcctgtcat cgacgccagc
     1081 tatactgtct ggctatggac actttcacct ggtcgctcaa gcctttttct tggaacgtcg
     1141 ctgaacgcat cgcgaaggag ctcgacctgc cgatgatggt ggcgatcgtg ctcgcccgca
     1201 ggggattccg cgattccgag caggtgcggg ccttcctgga gatctcgccg gcggtccccg
     1261 acccctttct cttctctgat atgagagcgg ctgtcgctgt cgttaaccag gccgctgcat
     1321 cgggaaggcg catcgtcata cacggcgact acgacgttga cggcgtctcg gccaccgcac
     1381 tcctgatccg cggcttggcc gaccttgggg tgcgggcgga gcactacctg ccgagccgct
     1441 tcgtgcaggg gtacggcctc tcggagaccg gcgtgcggga gatcgcggcc gggggagaag
     1501 cgctgctggt cacggtggac tgcggcgtca actaccccga tgaggtggca ctcgcgcgcg
     1561 agctgggcat ggacgtggtg gtcaccgacc atcaccaacc cggcgacacc ttgcccgatt
     1621 gccctctgat ccacccggtc cgcggcgggt atccacactc ggacctctgc ggagtgggta
     1681 tcgcgctcaa gctgttgcac gctctgcaca tcgaccgctc ctcggtccca ccggacagga
     1741 tccccgaacg tctgctggcg cacctcgatc tggtggctct gggcactgta gccgacatgg
     1801 tgccactgcg cggtgagaac cgccactacg tcgccgaggg ggtcgtcagg atcgcagcca
     1861 gcggcaagcc gggcctcaga gctctggtga cggtggccaa cggcgagggg cgtgtcgacg
     1921 ctcagacggt cggcttccgg ctggctccgc gcatcaacgc ggcaggccga ctcggcgatc
     1981 cggagatccc cctccggctg cttcttaccg atgacgccgc cgaagctgag acactcgcgc
     2041 aaggcctcga cgcgctcaac cgcgagcggc agagcgtcga ggcggtggtc ctcgaggaag
     2101 cgctggtggt ggtcgagagt ctagagcaga tgcccccgat cctggtagtc gcaggccgag
     2161 attggcacga aggcgtgttg ggcatcgtgg cctcccgtct cgtggagcgc taccatcggc
     2221 cggcggtcct gctctctctg gcagggggaa gggcccgcgg ctcagcccgc agcatccgcg
     2281 catacgacat catggcaggc ttgacggcgt gcgctcggca cctcacggtc ttcggcgggc
     2341 acccgcaggc agcgggcctc actctgcccg aagacatggt ggcggagttc aaatcggacc
     2401 ttgaacgcca cgcagccgag gcacttgtcg atgcagacct tctccctaca taccatcctg
     2461 acgccgtggc cgcgggggtg gcgctcaatc tggagacggt cgacgctctc tcccggttgg
     2521 cgccgttcgg tttcggcaac cccgcggtca aactgatcgc gctggcggcc cgcctggagg
     2581 acgcgcggcc cactcgtaag ggcgatcatc ttcagggcac gctggtggtc gacgaggtgc
     2641 ggacacgcgg catcgggttc ggtctggccg ggaagctgcc cgagttgatg gaagacggcc
     2701 tgcgggctca tgcgggcatc cgcttggagg cgagcgagtg gcagggcacg agcagggccg
     2761 aggtccaact gcacagtttc taccgagtcg cccccgacct aggcgagggc gccctcgggt
     2821 gctccccgat ctgcccgttc ctggacgatc tggaggctcc gcccgcctgc cccgcctgcg
     2881 cccatccctt ctctggggcc gcgttcaccg acgtgatggg tagggatctc cgtgatgcgc
     2941 cgggcaggct ctcgcacacc gcggagatcc tgtcgacggg tgaagacgcg gccatcctag
     3001 gggctgacgt caagcggcac ttggtgcggc tgagcggcga actccccctc agggaactcg
     3061 gcgtcgtcgg cacccactgc gtcagcaggc actgctggcg cacgaggctt gcgagcccac
     3121 gcggcgacac actcctcttc gccgattggg acgccgcgga gagaaggccg gaactgctgt
     3181 gtgcgcggag ccacgtgatc gtcgtggacc ccgtctaccg ggcctctcag tcggcgctac
     3241 tgcagcagat cgccgactcc ggcgcgcgcg ttcatctcct ctacggcgac gaagagagac
     3301 gggcaaccga ggaggagttg cgggtccgta tgcatccacg ctactggatg gtcgcgctct
     3361 atcgagcact gcgcaccgac accggctgtg acgacggact tttcgccggt gtgatgggcg
     3421 cctcgtggga gaaggactcg gtcatgccca cctcggaaga cctccgacgt gcctcgcagc
     3481 ttctgacgga gctgggacac gggtcgaacg gggggggagg ggctacaatg gaagcctcca
     3541 tgagtcccgc gtacacagcc gcagtagaag ccttcgagga ggcggtccgg ctatgtcgga
     3601 agacgtgacc caaaccccac ctcctgacga ggcgcaactc gatcttccgc atcgagagct
     3661 tctcgacgac ctcttccgcc agatcgtccg ctacaaccct acgttcgacc gggacatgat
     3721 cacgcgggcg ttcacggtgg ctgtaagaaa acacaaggac caacgaaggg cgagcgggga
     3781 agactacatc ctccaccctg tcggcacggc caccatatgc gccgaccttc ggctcgactc
     3841 acccaccgtg accgctgcct tgctgcacga cgtggtagag gacaccgacg tgtccgtggc
     3901 cgaggtcgtc gccgagttcg gggaagaagt cggtctcctg gtcgacggcg tcacgaagct
     3961 cacccagatg tcttttcgca gcgtcgaaga ggagcaggcc gagaacttcc gcaagatgat
     4021 catcgcgatg gccaaggaca tccgcgtgat cctcatcaag ctggccgacc gcctccacaa
     4081 catgcgcacg ctcagctacc tggggaaaga gaagcagatc cagaaggcca aggagaccct
     4141 ggagatctac gcgccgctgg cgcaccgtct ggggatcgag tccgtccgct gggagctcga
     4201 agacctcgcc tttgccaccc tgcatcctcg caagtactcg gagatccagc acatggtctc
     4261 gcagcgccga gccgaccggg aggagtatct ggaggaggct cgttcgtatc tcaagtccga
     4321 gctggagaag gtcaagatca acgctgagat cctgggccga gcgaagcact tctactccat
     4381 ctacgacaag atgacccgca aaggcaaaga gttcaacgag atctacgacc tcaccgccat
     4441 gcgggtcttg gtggcttccg tgcgcgagtg ctacgcggcc ctgggcatca tccactcctt
     4501 gtggaaaccc ctcccggggc gattcaagga ctacatcgcg atgcccaagt tcaacatgta
     4561 ccagtctcta cacactacgg tgatcggccc gcagggcaag cccctcgaga tccagatacg
     4621 cacctcggag atgcatgaga ccgcggagta cggcatcgcc gctcactgga tctacaagga
     4681 caaggacagg agcaaggagc gggagaagga ccgcatcgac cggctggctt ggctcaggca
     4741 gatgatggag tggcagtcgg acaccaagga cgccacggag ttcatggaat cactccggat
     4801 cgacctgctg caggatgagg tgtacgtctt caccccgaag ggagaggtga agagcctcgc
     4861 ggttggcgcc acccccgtcg atttcgccta cgccatccac acggacgtcg ggcaccgatg
     4921 cgtcggagcc aaggcaaatg ggaggatcgt tccgctcacg taccgtctgc aatcaggcga
     4981 catcgtcgag atcctcacca cgaagaacac ccagggaccg tcacgcgact ggctcaactt
     5041 cgtccagact tcgggggccc gcaacaagat ccgccaatgg ttcaagagag agcggcggga
     5101 ggactcggaa caccttggca aggaggccct cctcgagagc ttccgcaagc acggtctgcc
     5161 cgcgcagaaa ctgatgggct ccgacactct ctcgcaggtc atgaaggaca tgaacttcca
     5221 gaagcgggaa gacttcttcg tgagcatcgg agcgggcaag accagccctc agcaggtcct
     5281 cacgaaggtc atgcagcaca tggggcaggg gacgggggcc gtcaaggagg agctccctcc
     5341 gatctcgata ctgccctcgc cccggcgact tcccacctcg gccgacatag gggtcagagt
     5401 ggagggcatc gacgacgtcg ccatccgcat gcccgagtgc tgccgaccgg tcccgggaga
     5461 cgagatcgtg gggtacatct cgctgggcaa gggcatcacc atccatcgca aagactgtcc
     5521 caacgtgcgc gccctagaga gacatccgga gcgattcgcc gaagtgtcgt gggaccaagc
     5581 ggttcgcgca cccttccgcg ttgagatcca agtggaggcc tatgatcgca gccggcttct
     5641 cgaagacatc tctcgcacct tgagcgagag cggggtcaac atcctggccg cgcatatcca
     5701 gaccaccgac gacaacacgg tcaaagaccg gttcgtgttc gaggtgcccg gcgtcgagta
     5761 cctggattcg gtacttcagc gcatacggat gatcgacacg gtctacgacg cctacagggt
     5821 cacccctcat tagaagggcc cacaccctgc gagaccgacc ttgggcccgt gtggtgtggg
     5881 tcccgaccca caatgcccgc gtagtcggcc ccgccccgag gcccgcgtag tcggtctcga
     5941 cccgaggcgc tgccgccggc atgcgcgctt gttgcgccta tcgagcgcgt gccgccgggc
     6001 gctcgcaccc cctctggtat cattgccttt ctgtcttgta cacgtggcac gaggtatcgg
     6061 cgattatgag aatcctgacc gtcccagtag gtccgcttca ggcgaactgc tacctggtgg
     6121 ccgacgacgg cggcatcgca ctcgcgatag acccaggcgg agagcccgaa cgactcatct
     6181 ctgcgctgga gagggagggg atgcgtctta cgcatatcct tctcactcat gggcatttcg
     6241 accatctggc gggggctgcg ggattggcgg agctcaccgg cgctcgaatc gcctgctcgg
     6301 agggaaccgc gcccatgttg cgcaaccctg acgactacat cccgttcccc ggcttcggcg
     6361 gcatacccag tcgggaaccc gatgtgcttc tcgctgacgg ggatatcatc agcgtgggac
     6421 agctcgaggt ggagaccgtc accacccctg gtcactcgcc gggcgacgtg accttcgccg
     6481 tggacggggc cctcttctgc ggagacctgc tcttctatcg ctccgtcggg cgcaccgatc
     6541 ttcctggagg tgacttcgac atgctcctgc agtccgttcg cagactcgtg gaccgcttcc
     6601 ccggcgaatc acgcgtgtac cccggccata tgcagcccac gtcgctacgc gacgaggttc
     6661 gcgccaaccc cttcctctct ggactctcct cccgtggctg atcgaccgac ggccccgaaa
     6721 ggcacctacg acatcctccc caaccagcag gccacgcgcc gctgggtagt ggccagagcc
     6781 accgaggtct tccgtcgcta cgggtatgga cgattggaca cccccgtgtt cgaggagact
     6841 cggctgttcg cccgtggagt gggggagtcc accgacatcg tccgcaaaga gatgtacacc
     6901 ttcacggatc tcggcgggcg cagcatgact cttcggccgg agggcaccgc tccgtgcgcc
     6961 cgcgcctatg tcgaacacgg catgcacaaa ttgccgcagc cggtgaagct ctggtatcac
     7021 tgccccatgt tcaggtatga gagccctcag tcgggccgat atcgccaaca ccaccaattc
     7081 ggcatcgagg ccttcggctc ggattcgcct gcgctcgacg gggaggccat cggagtgctc
     7141 gcggagttgt atcgcgacat cggcctcgct cagaccgaac tccgcatcaa ctcgatgggg
     7201 tgccggcagt gccgccccac ctactctgag gagttgcgct cgttcctgac cgaacaccgt
     7261 ggtgagttgt gtccggagtg tctcgagcgt tcccgcctga accctatgcg caccttcgac
     7321 tgcaaggtcc cagggtgcgg tgaggtgctg cagaatgccc caagactcac cgactttcta
     7381 tgccccgact gcgcggcgca ccacgaggaa gtgaagaagt atctgggcct gcaggggatc
     7441 gactatgtag aggaccaccg gcttgttcgc gggatggact actacacccg tacgaccttc
     7501 gaattccagt ctgccgtcct cggggcgcag tccggggtgg gtggaggagg gcgatacgac
     7561 gacctggttg agaccatcgg cggccctcct acacctggag tgggctttgg aaccggtgtg
     7621 gagaggatct tgctggcgtt ggcccgctct cgtgacgacc tgcctgccga ggcgtcccct
     7681 gcggcctacg tggtcgctct gagcgactcg acgcgggccg aagccttcag attcgcccac
     7741 gaggcacggc gtctcggggc ggcggtcgac atcgactacg ccgggcgtag cgtgaagggt
     7801 cagatgaagc aggccggccg cagtcgagcg cgctacgcag tcatcgtagg cgaagacgag
     7861 gtcgctgagt cttcggtcac cctgaaagac ctcggcacgg gtgaggagcg cacgctcccc
     7921 cgtatcgatg ctatgactct catcgttgaa gctgatgaca agggcggatc atgacataca
     7981 ggacacactg gtgcggcgag atcggagaac agacggtggg ccggcgtgtc tccgttgcgg
     8041 gatgggtgca tcgacgccga gaccacgggg gtctcgtctt catcgatttg cgcgaccgga
     8101 gcgcgctggt gcagttggtg ttcgaccccg aacagcatcc ggtcgctcat gcggcggccc
     8161 atgacctgcg tggagagtac gtgatcgcag ctaccggaca ggtcgtcgcc cgcggcgcgg
     8221 accaggtcaa tccgagactg gcgaccgggt cggtggagat ccacgtggat gaactcaccg
     8281 tgctggcaga gtccaagaca ccgcccttct tccccgaaga tgacgtggac gtcgatgaga
     8341 cgctccggct caagtacagg tacatcgacc tgcgtcgcca gaggatgcag cacaacctca
     8401 tgctgaggga tagggtagtg cagtcgatac gacgctacct ggggtccgat gggttcatag
     8461 agatcgagac tcccatcttg acgaagtcca cccctgaggg agcacgagac ttcctcgtgc
     8521 ccagccgcct tcgcccggga gacttctacg ccctccctca atctccgcaa ctcttcaagc
     8581 aactcctgat ggttgccggc ttcgaacgtt actaccagat cgcccgcgcc ttccgcgacg
     8641 aggatctgag ggccgaccgc cagttggagc acacgcagat cgacgtagag atgagtttcg
     8701 caagcgagac cgagatccag gacctcatcg aagggatggt ctggcatgcc ttctccgaga
     8761 ccctgcaggt agacctgccg cgaccctttc ctcgcctcac ctatacggag tcgatgctca
     8821 gattcggcag cgacaagccg gacacgcgct tcgggatgga gatcgtcgac gtgtccgacc
     8881 tcgtcggtga cgcggacttc aaggtcttcg cggacgccgt tgcgggtggg ggagcagtcc
     8941 gtgggctccg cgccgaggga gccgccggct tctcgcgcaa ggacgtggac gaactcacca
     9001 aggacgccgc cgtctacggg gcgaaaggcg tggcgcccat atgggtggag gaggggggca
     9061 tccgttctcc tatcgcgaaa ttccttggag aggaagcgac ggctgccgtc gtcgcgcgca
     9121 tgcacggccg cccgggcgac ctcctgcttt tcgtcgccga cgcgctcgcg atcgtcgaac
     9181 cggccctcgg ggcgttgcgc ctccgactgg ccgatctcct gggcgtgacc cgcgagggct
     9241 ggagcttcct ctgggtcgtc gacccgccga tgttcgagct cgacgcgcgc gaaggccggc
     9301 tgaaggctca gcatcatccc ttcacgaaac ctcgggtgtc ctgtcccgag gacctggccg
     9361 agcaacctct gcgcctgggt acagaggcgt acgatctggt cctcaacggt gtcgaattgg
     9421 gaagcggctc catccgcatc tcggatccgg ccctacagcg ggcggtcttc ggggccttgg
     9481 gcctcgacga cgacgagata agagctaagt tcggcttcct cgtagaagcc atggatttcg
     9541 gcatgccgcc gcacggaggc atggctctcg gtctggaccg cttcgtgatg ctcatggccg
     9601 gggagacgtc gatacgcgac gtcatcgcat tccccaagac tcagagcggt tcgtgccctc
     9661 tcacagaagc cccatcctcc gtctcccgtc agcagctcga agagttgcac atcaaggtgc
     9721 gttgaacgcg tgaccggtga cgctcctacc cgaagtgacg gcgcccgaag gccggtggaa
     9781 ggtacgccgg ggtttttgtc gaaccaatat ggctgtggta cagttggctt gctcgaccgt
     9841 gtgcgtgatc cgctcgaact tgagccaaca catattcctt gggaggcgtg ttctctcggt
     9901 cggcagagct cgccgaggac agtcacccac ctgcttacgc aggttcaagg gacaggcgga
     9961 cacggcaagg tggagcactt ctcttcttcg gaggaaccgt atgcagcgac atcgtaaggc
    10021 agtctacccc caggtctcgc tcgtggttct ggtcatcgcc gtggcgctcg ttcttgcggc
    10081 gtgcggcgga ggaggcgggt cgggcgcgga agaaacgtct acgacgatcc ctccggcgtc
    10141 acccacgact gccggtgcgg ccgcaccgac gaaagcgttc gccgacctgg tgggcacgac
    10201 cctcagcacg accgaagaca cgccggaaga cgtgggcaat agcctcgacc tccacgttcc
    10261 catggtgttg gccttcttcg tcaccggcgg caccgacgac gccatcgtcc tcgaacagct
    10321 cgacaagttg gcgctccgct tctcggatgt ggacttctac acgtacgact acaaggtgcc
    10381 cgccgcttac ggtgaccttg ccgggcagct gcacgtggac tacccgcccc aagtgacctt
    10441 cgtcgactcg aacggcgtca tccgaggcgt cacgagtggc tacgccgacg aaggcaccct
    10501 caaccaactg gtggccaaca tccgctagag cacggcatac cggtggacca tttctctcgg
    10561 gccggccgtg tggccggccc ttcgcatctc gtttcagata tgacaccgcc aagtgacact
    10621 cccacatccc cccgcgcgta ctccgaggcc gtgctcgccc accttcggac accggcgcac
    10681 gcgcgccgcc tgcccttcgc ccatgcggag ggcagagccg ggagcagggc gtgcggagat
    10741 ctcgtgcaca tccagctgca ggtagaagct ggcgccatag tcgatgccgg cttccaggcg
    10801 ttcggctgtc cggctacgat cgccacggcc tctgagatag tcacgcaact gccgggcctc
    10861 gacctcctcg aggcggctca cctcaaccac gaggatgtgg ccgcacgtct ggccctagcc
    10921 gagcacaagc gtggctgttc ggaggtcgcg gccgatgccc tctacggcgc cctcgaggat
    10981 tgggtgcgga ctccggccgc catctcccgc ccaggggtgc agcgccaggg tgcagtcctc
    11041 gtcgccatga gcggcggcgt cgacagctcg gcggccgccc tgatcctcaa acaggagggc
    11101 ctcactccag tgggggtcac cttccgcctg tggagcgatc cgacctgctc gtcgggaggc
    11161 ggatgctgca gccccgagac tgtgcttcga gcccggcgag tcgcccatgc cctgggcatg
    11221 gcccacgtga ccgtggacct gaccggggag ttctacgacg tagtcgtcga cgactttgtc
    11281 caggaatacg cggccgggcg cacgccgaac ccctgcgtgg tgtgcaactc gcggctccgc
    11341 ttctctgccc tcgcacagat cgccgaccgc ctggggatcg ataagatcgc caccggtcac
    11401 tatgcgcgcc tcctgggctc gcctccacgc ctgcatcgcg ctctcgatcc cgagaaggat
    11461 caggcatacg tcttgtcgcg ggtggatccc ggattgctcg aacgagtcgt gctccccttg
    11521 ggcgaactca caaagccgga ggtgcgacgg ctggcgcgcg atgcgggcct cgagacacac
    11581 gatgccaaag agagccaaga gatctgcttc atccccgacg acgactaccg ccgatttctg
    11641 gccgagagac tgggagtcgc cgggggcccc atcatcgacc ggcagggaag ggtccttggc
    11701 gagcaccggg gaacgtaccg gttcaccatc ggccagagga agggactcgg cttggcggcg
    11761 ccagagccgc tgtacgtgtg cgccatccac gacgatggcg cggtggtagt gggcacgcgc
    11821 gacgacctct tggtaggcga agtcgaactg cgggacgtgg tggcgcactc cacggccgtt
    11881 ccggggaagg atctcacgat acagttgcgt tcgagcggcc tagtggtgcc ggccacggtg
    11941 accgtagagg ggcgccgctc gacggtccgc ctcgcggagc ccgtgtccgg cgtggccacg
    12001 ggccagacgg gcgttgtgtt cgcgggcgac cgcgtcgtgc tcgccggcgc gatccacgag
    12061 acccagcccc tccgtgcacc cgggagcagc cggcaagggt agccggggcc taccggcagg
    12121 ctcttcgggg ccctatgcta tagttgtacc tgcacaacgg cgggaccagg gctctcgcgc
    12181 cagaaccgga tcacgaggag gggagcgtca tggaatggga tgcggcagca ttcatgaaga
    12241 tcgccatcgg cctcttcttc cttgccttcg cgggcggcct caccgtcatg cttgtgcgcc
    12301 tcgccgtggt gttcaaggag atcgcggcga tgctggctga tgtgacgaag gagttcctgc
    12361 cgatactgtc tcgtcttcag accaccgtcg acgaagtcaa ctccgaactg ggcaagattg
    12421 atgagatcac cggttccgtc gtcagcgtca ccggggcgat agagagcacg acctccgccg
    12481 tacagagcgc gatatctgcg ccgtttcgga aggtggccgg agtctctgcg ggtttgagcg
    12541 aggctctgtc atccttcctc tctggtcgcc gaaaggagca ctgatgggca agttcgcgtt
    12601 cggactcact gtcgcgacgg ccgccgcgct cggggccgcc gctgctttgg cacaccgcat
    12661 ctccaccgag accggcaaga gcttctcgga agctctcaac gaagtgccgg ccgaagctga
    12721 gcgctacctc gaggagatac tggcgcgtgg gcgagaggcc tacgacgccg gccgaaccgc
    12781 cgccgcggag aagcaggccg agatcaccaa ccgcctcggc gagcgactct gaggctgcgc
    12841 agccagaccc tccggccttg atgggcgcca ccacagacac ggtcactctg accattccgc
    12901 agcgcgagga tcttctaccg ctcttcagga tgatcctagg aggtattgcg ctgcgcagga
    12961 acctgtccct ggagactctt gacgacctgc aacttgcggt cgacaccgtc ctggcggaag
    13021 acgaggcgca gcggggagac gtatccatgt cggtggaatc ggaggcgggc gtgcttcgga
    13081 tccgcctgag ccccttgaca agccgggact tgcgggaaac cctccttcaa ggttccgtgc
    13141 ctgaaggggc gggcgatcgg tgcctcgacg tgtgcttgat cctgcgctcg ctggtcgatg
    13201 acttcgcgat ccatgacggg accgatgatt cgttcgccgt cgtgctgcgg aagctgaccg
    13261 gatagtcgga tggtcgacgg gggtaccgca aacgataccg cgcgcgccgg gcttttcgcc
    13321 gtgccgctgg gcgtcgtagc cgagaaggaa ctcttgcggc gcatccaccg agaggccgac
    13381 ctcgcggcac gcgaggaact catcacgcgc tacctgcctt tggtcaagag cctggcgcgc
    13441 cgcttcgctt ctcgcggtca gccggtcgag gacttggtcc aagtgggctc catcggcctc
    13501 atcaaagcca tcgaccgatt cgacatcgag cgcggcgtcg agctctcgac ctacgctacg
    13561 cccacgatcc tcggcgagat caaacgatac ttccgagaca agggttgggc cgtcaaggtg
    13621 ccccgagccc tgcaagacct gaacatacgt ctcaacaagg tgatcgaaca actcaccgtg
    13681 gaactgaacc gctcacccac gatagccgag ctggcagtgg catgctccgt caccgaggaa
    13741 gatgtgctcg aggcccttga gagcgggcgc gcctacaact ccctctctct gttctcgagc
    13801 ggaggctccg acgacgaaga ctccctcgaa ctcctcgact acctcggcaa ggaagagtct
    13861 gaatacgaac tcgtggagca acgacgatac ctggcgccag ccatgggccg actcgatgaa
    13921 cgtgaacgtc tcatcctgca cctcaggttc ttcgagggca tgacccaaac ccagatcgct
    13981 tcgcgcgtgg gcatctcgca gatgcacgta tcccggctca tccgcaagtc gatcgacatg
    14041 ctccgccaga ccatgagaga gcagggagaa gagtcctcgc cgctcgacta acgcgagggc
    14101 ggagtccaga gacaccgaaa ctccgagata cctgaccggg aagaggttgt gcgtgaagac
    14161 acgcgatctt cgtaagttgt tcttggacta cttcgtcgag aacggccacc tgctgatgcc
    14221 gagcgcgtcg ctggtgccat atcgcgaccc gtccgtcctg ctgacaacgg ccggcatgca
    14281 gcagttcaag ccctacttcc tggggatggc tgaggctccc gccacgcgca tgaccagcgc
    14341 tcagaagtgc ttccgcacca cggatatcga tcgagtcggt ttcacggctc gccactgcac
    14401 cttcttcgag atgctcggca acttctcagt gggagactac ttcaaggaag gcgccatccg
    14461 tttcggctac gaactctcca ccgagagact aggtctcgac cccgccagga tctgggtgtc
    14521 ggtgttcgaa ggagatgccc aggtcgaggc ggacgaagag gccatgcgcc aatgggagca
    14581 ggtcggtatc cctcgtgaac ggatggtggc tctgccgcgc agcgagaact tctgggggcc
    14641 cccgggacct acgggcccct gcggtccttg ctccgagttg tactacgacc gaggctcaga
    14701 taggggctgc ggcagtccgg actgcagacc cggctgcgac tgtgaccggt tcctcgagta
    14761 ctggaacctg gtgttcatgc aatacaacat ggacgaccaa ggccgcctga cacccctgcc
    14821 ctccaggaac atcgacacgg gcatggggct ggaacgcata gcggcgctca tgcagggtgt
    14881 ggattcggtc ttcttgaccg actccttcta cccgctcatc caactgggcg aggagatctc
    14941 gggactcgcc tacggaggat ccgagcgcgc cgatgccgcc ctccgggtcc tggcagatca
    15001 ctcccgatcc atgtcgttcc tgatcgccga cggcgtcctc cccggcaacg aagggagggg
    15061 gtacgtgctg cgcaggatca tccggcgtgc cgcccgcttc agtcgcaacc tggagatgac
    15121 gcccccgttc ctggggcgct tcgcggagcg gaccatcgag ctcctggcgg atgcctatcc
    15181 cgagttgcac gaacgccgtg agacgatcat gcgcatcgtc aattccgagg aggagcgctt
    15241 caaccgtacg ctcgaccaag gcctcgtact cttggatgag gagatacggc gggcccgggg
    15301 cggcggcacc aagatgttct cgggcgcggc ggccttcgtc ctgcacgaca ccttcggctt
    15361 cccggtggag gtcacccgcg agatcgtgga ggagaagggc ctgtctctcg acttggacgg
    15421 tttcgacgcg gcgatggagg gacaacgggc acgggcacgg gccacgagca aggagctcga
    15481 ctcgctacat gaggcggcgg tccatcttgc tcgggagtcg cagaacgcca ctgaattcgt
    15541 gggatatgct caggacgagg tgttcacggt catcgacagc cttcaggagt tcgaggacgg
    15601 tgacgtcgtg ctcactcttc gcgagagccc gttctacgcc gaaggcggag gccaaacggc
    15661 cgacatcggc tggatcgaga cggacgcggg cagagccgag gtgcgggatg tccagcacca
    15721 tggagccgtg caggtgatcg tggcacacac aactgcgggg tcgcttgaac agggagttcg
    15781 cgccaaggcc gtgatctcgt cagcgcatcg ccactcggtg gccgccaacc acacgggcac
    15841 gcatctcctg caccacgcgc tccgctcgac tctgggaaag gatgtgaccc aggccggctc
    15901 ctcggtgcgg gccgacaagt tccgcttcga tttcgcgttt cacgagcctc tcgggtccga
    15961 ccgcctcaca gagatcgagg agatcgtcaa ccggaagatt gtggagaacc accctgtcag
    16021 agccttcacc acaagccttg accatgccag ggatctagga gccaccgccc tgttcggcga
    16081 gaagtacggc gacttcgtcc gcgtggtgga aatcgacgac ttcagtcggg agttgtgcgg
    16141 gggaacccac gtgcattcca cgagcgagat cggcatcttc aagatcctct cggaaggtag
    16201 cgtgggtgcc aacgtccgcc gcatcgaggc cgtcacgggg cggcgggcgg tggagtacta
    16261 ccgctcgcag gattcaaccg tgtccgctgc cgcagggatg ctggccgtcc aagaagatgc
    16321 actgctcccc gctgtgaaca agctccagac tcgagtggcc ggcctgacga gggaggtggg
    16381 gcaactcctc gccgcgaagg ccggcgacct gacctctgcc gtcgttgaag cggcaatcga
    16441 ggccgacggc gtcaaggtag cagtcgcaca agtggacgca cgggacaacg atcacctctt
    16501 ggccctgctt gaccaggtgc gggatcggct ggcgccggcc atcgttctac ttgcgacggt
    16561 ctccgcgggg aaggtcacca gtgccgccgc cgtaagcaac ggggtcgacg ggtctgacgc
//
LOCUS       DASRMJ010000057         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_474831, whole genome shotgun sequence.
ACCESSION   DASRMJ010000057 DASRMJ010000000
VERSION     DASRMJ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474831"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VFD74_01690"
     CDS             <1..1333
                     /locus_tag="VFD74_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48299.1"
                     /translation="IITAILTLAGVMAMMFWISPLLAVISLVTVPLSVLVTVLIAKRS
                     QKQFALQWARTGTLNGHVEEMYTGHNIVKVFGRQKEAIEAFDEQNEQLYRASFKAQFI
                     SGIIMPSMMFIGNLNYAAICVIGGFRVASGTMTLGDVQAFVQYSRQFTQPITQTASVA
                     NVLQSAVASAERVFELLDEAEESPDVADPVVLTEVKGHISLKGVSFRYLPDTPLIEDL
                     DLEVQPGQTVAIVGPTGAGKTTLVNLLMRFYEIDEGSIFIDDVDTRNMTRPDLRRLFG
                     MVLQDAWLFGGTIRQNITYGNEKATEEEILAAAEAAHVDHFVRTLAEGYDTRLDDDSA
                     HVSQGERQLLTIARAFLVDPQILILDEATSSVDTRTEVLIQKAMSRLLCNRTSFVIAH
                     RLSTIRDADVILVMNEGRILEKGTHEDLLARGGFYCELYNSQFADALAEAS"
     gene            1351..2493
                     /locus_tag="VFD74_01695"
     CDS             1351..2493
                     /locus_tag="VFD74_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZK48300.1"
                     /translation="MHGATFSPEQLSMVQMVRELMLEQVAPRTQEIDERAEFPSWVAE
                     LFQRYDLFGIAVPEEYGGIDGSLVTQCAVVEQVGRVCGSCSMILGVQCLGSAPIALFG
                     DDAQKPKWLPALATGEKRPAFGLTEPNAGSDVRSMTTRAVAKDGGYLLNGRKSFISHA
                     NVADVLVCFAKVKGVEGDGITAFLVETDRQGFEVDKIEHKMGLRGSPTCSLVLEDVWV
                     PAENVLGGVGNGMEVALGTLHRSRVCSAAQSLGVAQGALDLSAAYIKDREQFGRTLSR
                     LPGVQAIVGQMVTEVEAARSLVFTAAARYDQGVGEPGRFSSMAKLFASDVTMKVTTDA
                     VQIMGGYGYMVEYGVERFMRDAKVFQIFEGTNQLHQMGVARSYLGV"
     gene            2513..>2829
                     /locus_tag="VFD74_01700"
     CDS             2513..>2829
                     /locus_tag="VFD74_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HZK48301.1"
                     /translation="MTTLLWQPSAERVASANMTRFREFVNTRHALALDSYDSLYRWSV
                     DDIPAFWAAAWDFLGIQASAPYETPVEDLSMFPGTHWFPGARLNFAENLLRFRDDRTA
                     FVFV"
BASE COUNT          493 a          903 c          910 g          523 t
ORIGIN      
        1 gatcatcacg gccatactca ccctcgcggg ggtcatggcg atgatgttct ggatcagccc
       61 gctgctcgcg gtgatctcgc tggtgaccgt cccactctcg gtgctggtca cggtgctcat
      121 cgccaagcgc tcgcagaaac agttcgccct gcaatgggcg cgcacgggca ccctgaacgg
      181 acacgtggag gagatgtaca cgggtcacaa catcgtgaag gtcttcgggc gtcagaagga
      241 ggcgatcgaa gcgttcgacg agcagaacga gcaactctac cgcgccagct tcaaagcgca
      301 gttcatctcg ggcatcatca tgccgtccat gatgttcatc ggaaatctga actatgccgc
      361 tatctgcgtg atcggcggct ttcgtgtcgc cagcggcacg atgacgctcg gtgacgtgca
      421 ggccttcgtg cagtattcgc ggcagttcac gcagcccatc acccagacgg ccagcgtggc
      481 caacgtgttg cagtcggctg tggcctcggc cgaacgcgtc ttcgagttgc tcgacgaggc
      541 cgaggagtcg cccgatgtgg ccgaccccgt cgtgctcact gaggtgaagg gtcacatcag
      601 cctcaaggga gtctcgttcc gctacctgcc cgacacgcct ctcatcgagg atctcgacct
      661 cgaggtgcag ccggggcaga cagtcgcgat cgtggggccg acaggagccg gcaagaccac
      721 cttggtcaac ctgctcatgc gcttctacga gatcgacgaa gggagcatct tcatcgacga
      781 tgtcgacacc cgcaacatga cgcgccccga cctgcgtcgc ctcttcggca tggttctgca
      841 ggacgcgtgg ctgttcggtg ggaccatccg ccagaacatc acttacggga acgagaaggc
      901 caccgaggaa gagatcctgg cggctgccga ggcggcccac gtcgaccact tcgtgcgcac
      961 gctggccgaa ggctacgaca cgcggctgga cgacgactcg gcgcacgttt cgcagggtga
     1021 acggcagttg ctcaccatcg cccgcgcgtt cctggtggat cctcagatcc tcattctgga
     1081 cgaagccacc agctctgttg acacccgcac cgaggtgctc atccagaagg ccatgtcgcg
     1141 tctgctgtgc aaccgcacca gcttcgtcat cgcccaccgg ctctcgacca tccgtgacgc
     1201 cgacgtcatc ctggtgatga acgagggtcg catcctggag aagggcaccc acgaggacct
     1261 attggcgcgc ggcggcttct actgcgaact ctacaacagc cagttcgcgg acgcgctcgc
     1321 cgaggcgtcg tgaaggagtc ccccgacccc atgcacggcg ctacattctc ccctgaacaa
     1381 ctgtccatgg tccagatggt gcgcgagctc atgttggagc aggtagctcc acgtacgcag
     1441 gagatcgacg agcgggccga gtttccctcc tgggtggcgg agctcttcca acgctatgat
     1501 ctcttcggga tcgcggtgcc cgaagagtat ggcggtatcg acggctcgct ggtcacacag
     1561 tgcgccgtgg tggagcaggt gggccgggtg tgcggctcgt gctcgatgat cctgggggtc
     1621 cagtgcctgg ggtccgcccc tatcgccctg ttcggtgacg atgcccagaa gccgaagtgg
     1681 ctccccgctt tggcgaccgg agagaagcga cctgcgttcg gactcactga gcccaacgca
     1741 ggctcggatg tgcggtccat gaccacccgc gccgtcgcca aggacggcgg ataccttctc
     1801 aacgggcgca agtcgttcat cagccacgcc aacgtggccg acgtcttggt gtgcttcgcc
     1861 aaggtcaagg gagtggaggg cgacgggatc accgcgtttc tcgtggagac cgatcgccag
     1921 gggttcgagg tggacaagat cgaacacaag atgggcctgc ggggttcgcc tacgtgctca
     1981 ctcgtcttgg aggacgtgtg ggtgcccgcg gagaacgtcc tgggaggcgt cggcaacggg
     2041 atggaggtgg cgctgggcac tcttcaccgg agccgtgtct gctcggccgc ccagtcgctc
     2101 ggggtcgctc agggtgccct ggacctctcg gccgcgtaca tcaaggatcg tgagcagttc
     2161 gggcggacgc tctcgcgact tccgggcgtt caggcgatcg tgggccagat ggtcaccgag
     2221 gtggaagccg cccgcagcct ggtcttcacc gccgcggccc ggtacgatca gggagtcggg
     2281 gagccgggca ggttctcctc gatggccaag ctcttcgcca gtgacgtcac catgaaggtg
     2341 accaccgacg ccgtgcagat catgggtggc tacggctaca tggtggagta tggtgtcgag
     2401 cggttcatgc gtgacgcgaa ggtgttccag atcttcgagg gcacgaacca gctgcaccag
     2461 atgggcgtgg ctcggtcgta cctcggcgtg tagagaccag gaggagatca tcgtgaccac
     2521 gctcctatgg caaccctccg cggaacgcgt cgcttccgcc aacatgaccc gtttccggga
     2581 gttcgtgaac acgcgccacg ctctggccct cgattcctac gactccctct atcgctggtc
     2641 ggtggacgac atccccgcct tttgggcggc ggcctgggac ttcctgggga tccaggcatc
     2701 ggcgccctac gagaccccgg tggaagatct ctcgatgttc ccgggcacgc actggtttcc
     2761 cggcgcccgc ctcaacttcg ccgagaacct gctgcgcttc cgtgacgacc gcaccgcctt
     2821 cgtgttcgt
//
LOCUS       DASRMJ010000058         3237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_221211, whole genome shotgun sequence.
ACCESSION   DASRMJ010000058 DASRMJ010000000
VERSION     DASRMJ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221211"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VFD74_01705"
     CDS             complement(<1..970)
                     /locus_tag="VFD74_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48302.1"
                     /translation="MNIGVNARIGPAGRAAALAAGIPICAAATLALLYAVGITPSADA
                     AGPQVGPRAELVGSQAELLITMPLEPSMGSTLFTLPWGDKPGQVGLTQRAEEQSRGPE
                     AFALAPDGRVAVLDSVNSRLLLLAPSGASLATAPVALAAPRFLAANAEEVFVLDADED
                     LVLLTYSWEGTLLQTLPVGPFEEPVTSLLLDPQGRPLIETGHEATAAAEAWAPGNAAP
                     TGVRGEMHGRPVQAAPGRSVSTRMARGGAPRVEETGPDGSSARGWDIALGRGARIDHM
                     VSLDTDAQGRILLGLRVLPGRSASGAVRQGSGSAAGGATESATGAAG"
     gene            1185..1976
                     /locus_tag="VFD74_01710"
     CDS             1185..1976
                     /locus_tag="VFD74_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HZK48303.1"
                     /translation="MIILFFGDVVGKPGRSLLVEQLARLRAHYGADAVVVNGENASQQ
                     GVGISPDVADSFFAAGVDVITTGNHVYRRREIYEYLDRQPRIIRPYNFLPSNPGRGVT
                     VVDTKAGRLGVVNLSGQLFLHAARSPFEVVGEALEQLHGVRNVLVDLHAEATSEKVAM
                     GHYLDGRVSAVVGTHTHVRTADARVLPGGTAYITDAGMCGPRDSVIGMKKETIIERFL
                     TQMPARFEPATGDVWLEGVVIRVADNGKAESISLVESNGRAGMAY"
     gene            complement(2030..>3237)
                     /locus_tag="VFD74_01715"
     CDS             complement(2030..>3237)
                     /locus_tag="VFD74_01715"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HZK48304.1"
                     /translation="LSPYNVVRVDFGKTFPDDTDAENRYTRAARLLRDWKSENILVRD
                     PQPAITIVEESFIGPDTRPRTRRGFLALVKLQDFSEGVVFPHEATLSGPKRDRYRLMD
                     ETCMNLSPVFMLYSLPDDSVMTTWDAVSGGVKAAASMTDPAGNTTSLWPTSDPAVLSA
                     ATSRLNGVPLLIADGHHRYETALRYRAARHEAGDGDGPWDYALVYLCNMEDPGLAIFA
                     THRLLHGLNDDMIQGLRDTLSRFFTLEPLTDDPAKAEEAISDFLEARLNQGGAFGLYG
                     PSLGTAYGLALRDPNAPETMADDMHSQAYHRLDVAILHNVIFHELLGITSEDVAAGQY
                     VTFVKNWSRGFERLETGESQIGFFMNPIKLRQVYDVALGGERLPQKSTYFYPKLPTGL
                     LFHDLSGRD"
BASE COUNT          513 a         1050 c         1143 g          531 t
ORIGIN      
        1 ccccggcggc acccgtagcg gattcggtgg cgcctcccgc ggcggatccg gaaccctgac
       61 gcacggctcc actcgcgctc cgcccaggca gcactcgcag acccagcagg atccgcccct
      121 gggcgtcggt gtccagggat accatgtggt cgatgcgcgc tccgcggccg agagctatgt
      181 cccacccgcg cgcgctgcta ccgtcgggtc cggtctcctc tacccgcggc gcgccaccgc
      241 gggccatgcg cgtgctgacc gatctgccgg gagcggcctg taccgggcgc ccatgcattt
      301 ctccacgtac ccccgtgggc gctgcgttgc ccggcgccca cgcctcggcc gccgcagttg
      361 cttcgtggcc ggtctcgatc aacggccgac cctgtgggtc taggaggagc gacgtcacag
      421 gctcctcgaa ggggcccact gggagcgtct ggagtagagt gccctcccac gagtaggtca
      481 ggagcacgag gtcttcgtcg gcgtcgagca cgaagacctc ctcggcgttg gcggcgagga
      541 acctgggggc cgccagcgcc accggtgccg tagccagaga cgcgcccgac ggggccaaca
      601 gcagcagtcg actgttgacc gagtccaata cggccacacg cccatcgggc gccagcgcga
      661 atgcttcggg accgcgcgac tgttcctccg ctcgctgcgt gaggcccacc tggcctggtt
      721 tgtcccccca gggtagggtg aagagtgtcg atcccatgga cggctcgagg ggcatcgtga
      781 tcagcaactc agcctgcgaa ccaactagct cagcccgtgg accaacctgc gggcccgccg
      841 cgtcggcgga cggcgtgatt ccgaccgcgt acagaagcgc gagggtggcg gcggcgcaga
      901 tggggatccc ggcggccagg gcagccgccc gccccgcagg gccgatccgg gcgttgactc
      961 cgatattcat gttcgctcct tcctcgggcg tatgtagtgc ctcgaggcta ggctcccgat
     1021 ccaagcgtgg gccatacgac caaggtcggg ggttgccgag tgcgcgaagg cccgtccggc
     1081 ccgtcctgtc cggaccggca gtcaccggag gcaccgcttc cgtcgacgag gccggcgcgc
     1141 ccaggtacac tgttctttca gcagagggga tcggaggtgc gcgcgtgatc atcctgttct
     1201 tcggagacgt cgtcggaaag ccgggccgca gcctgctcgt ggagcaactt gcacgtctgc
     1261 gcgcccacta cggggccgat gccgtggtgg taaacggcga gaacgcctcc cagcaagggg
     1321 tgggtatcag tcccgatgtc gccgactcct tcttcgccgc aggggtcgac gtgatcacca
     1381 ccggcaacca cgtctaccgg cgccgggaga tctacgagta tctggacagg caaccccgca
     1441 tcatccgtcc ctacaacttc ctcccgtcga accccgggcg gggggtgacg gtggtggaca
     1501 cgaaggccgg cagactcgga gtggtgaatc tgagcgggca actgttcctc cacgccgccc
     1561 gatcaccctt cgaggtggtg ggcgaagccc tcgaacaact ccacggcgtg cgcaacgtgt
     1621 tggtggatct acacgcggag gcgaccagcg agaaggtggc gatggggcac tatctcgacg
     1681 ggcgcgtgag tgccgtcgtc ggcacgcaca cccacgtgcg tacggccgac gctcgggtgc
     1741 tgcccggcgg caccgcctac atcacggatg ccggtatgtg cggtccgcgc gactcggtca
     1801 tcggcatgaa gaaggagacc atcatcgagc ggttcctcac ccagatgccg gcgcgtttcg
     1861 agcccgccac cggcgacgtg tggctcgaag gcgtggtgat ccgggtggcc gacaacggca
     1921 aggcggagag catctcgctg gtcgagtcga acggcagggc ggggatggcc tactgagtcc
     1981 taggcgggcg cgggcggagg cgcgcagcgt gtcgcacagg gttccggcgt cagtccctgc
     2041 cgctcaggtc gtggaagagc agccccgtgg gcagcttcgg atagaagtac gtggacttct
     2101 gcgggagtcg ttcgccccca agggcgacgt cgtacacctg ccgcagcttg atggggttca
     2161 tgaagaaccc gatctgcgac tcgcccgtct ccagtctctc gaacccgcga ctccagttct
     2221 tgacgaaggt cacatactgg ccggcggcta catcctccga ggtgatgccc agcagctcat
     2281 ggaagatcac gttgtgcagg atggccacgt cgaggcggtg gtaggcctgg ctgtgcatgt
     2341 cgtcggccat ggtctccggt gcgttggggt ctctgagcgc cagaccgtat gccgtgccga
     2401 gcgagggtcc gtacaggccg aaggccccgc cctggttcag acgggcctcc aagaagtccg
     2461 agatggcctc ctcggccttg gccgggtcgt cggtgagagg ctccaacgtg aagaagcggg
     2521 agagcgtgtc gcgcagcccc tggatcatgt cgtcgttcag gccgtggagg aggcggtgcg
     2581 tggcgaagat ggccagccct gggtcctcca tgttgcagag gtagacgagt gcgtagtccc
     2641 aagggccgtc gccgtcgccg gcttcgtggc gggccgcgcg gtagcgcagc gcggtctcgt
     2701 atcgatgatg gccgtcggcg atgaggagcg ggacgccgtt gagtcggctg gtggccgccg
     2761 acaacaccgc cgggtccgat gtcggccaca ggctcgtcgt attgcccgcc ggatcggtca
     2821 tcgaagccgc ggccttgacg cccccggaca cggcatccca ggtggtcatg acggagtcgt
     2881 cgggcagcga gtagagcatg aagacggggc tcaagttcat gcaggtctcg tccatgagac
     2941 ggtatcggtc gcgcttcggg cccgacagcg tggcttcgtg cgggaacacc actccctcgg
     3001 agaagtcctg gagcttgacc agggccagga agccacggcg ggtgcggggg cgggtgtccg
     3061 ggccgatgaa cgattcttcg acgatggtga tggcgggctg cggatcgcgc accaggatgt
     3121 tttcggactt ccagtcgcgc aagagccggg ccgccctggt gtagcggttc tcggcatcgg
     3181 tgtcgtccgg gaaggtcttg ccgaaatcca cgcgcacgac gttgtagggg cttaggt
//
LOCUS       DASRMJ010000059         7681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_148530, whole genome shotgun sequence.
ACCESSION   DASRMJ010000059 DASRMJ010000000
VERSION     DASRMJ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148530"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFD74_01720"
     CDS             <1..407
                     /locus_tag="VFD74_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48305.1"
                     /translation="LGSPVVSVASVISESPHGALDSASPAAAPQSFESCVQRGFGLLA
                     RGEHEDGVAEFHEALALIDDREMKVRLHIVIGNAYRSQRMYEAAGAAYLQATLHTDHP
                     MLLEHLERIIAEMMDSDDVGAADTTSEPEKEE"
     gene            413..1369
                     /locus_tag="VFD74_01725"
     CDS             413..1369
                     /locus_tag="VFD74_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48306.1"
                     /translation="MARMDTDLLGLQIVALDSAAVVGEIDGLLIDDATMKVAGFLVDL
                     GLYEASVLSFGSAHAVGLDAVIVESADKITEISADPTLEALNERDIAISDAKAITRSG
                     RSVGTIGDFFVDTENGDVVGMEFLAADQTIYPRGIAVIPASVVFRLGRDIVVLDDDYD
                     QHFLKDGDSLERVGHSRSAEPAPVVTPEVVVAPEAATEAESPVLEVPEEAEAVAVEPA
                     AESELEPPVAPAAAIEPEPAADADTATEVETPAAETTPTVPADDLFATEHKHFLIGKR
                     VLRRIETPGGEVIAEEGDIVTFDTIEKAKSSDQLLILSLNVE"
     gene            complement(1391..2506)
                     /locus_tag="VFD74_01730"
     CDS             complement(1391..2506)
                     /locus_tag="VFD74_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZK48307.1"
                     /translation="MRIGFDARTIDWSGIGTYSRNLLQQFGKGELAGDDVEFVVFCPD
                     QKRESVPRGDRFSLVSTNIDPRSHSGRAVFARMVEQSGVDILHVPSHYAPIPVGVPVV
                     ATIHDVIPLIYPRTVRNPVARVRYRRQLDRVLGAARCIITVSQISLATLTAFAGVEAS
                     RVRVIHNGVSSQFHPVENEAERASARRRYGLPERFALWIGDFRPNKNLEFMVGGWAAL
                     QKVLDVPLTLAMAGAQEGEFRKIAHEAERRGLKDRVVFPGFVREDDLAAVYSSAAVFV
                     FPSLYEGFGLPPLEAMACGTPCVVSNSSALPEVTGRAAMMFNPTSVEQFVDCIHRVLT
                     EPDLNGNLRREGLCQSARFGWERAAAETIEVYRTMVA"
     gene            complement(2561..3412)
                     /locus_tag="VFD74_01735"
     CDS             complement(2561..3412)
                     /locus_tag="VFD74_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZK48308.1"
                     /translation="MSASGSLETLHLAELLQVGALFRKMGSIRLLFPGQRAMVVYLHD
                     GALSGLADSGRAWQLGDLLASLGLINSVDRQRLLTECTTRGKRLGQLVVEQGYLTKRE
                     VEIVLRRLMMQSLLYAAENEADATFEMHLGPVSQTSVMFPITDFLIELTSASDELDRL
                     RRILGSTGRTLWVAAHGDETGTIRDLPYRKVQVLAHVDGQKSPMEVAATSPFSPTETL
                     SILCELARSGVIDWVGHTPKPPLARVVTFPVAGRDSEKRTIAAAEAPETDVEAPERER
                     REPGSVW"
     gene            3712..4743
                     /locus_tag="VFD74_01740"
     CDS             3712..4743
                     /locus_tag="VFD74_01740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZK48309.1"
                     /translation="MSDGIAPQGKTVRSAPSPLFQKVEGVLDAGKVQVTKNWLARIIE
                     RIDDLASLEQFPTQESIKTSVELIEGLAAVLSDEDKLSGFEPGGKFYALAAGLGITDG
                     GGARGLVTLSQNMLELENAIWALLAGALRNEDRDLLRLVARLRVAMHGIHTASTEAYF
                     HRSNTELDRLAHTDQLTGLYNRRYMIQELERHGEMYKRYRHPFSVLMLDLDNLKTVND
                     TLGHPAGDAALKHLAMLLRVNIRDVDIACRYGGDEFVVLMPETEQNVVQIVGDRIAAS
                     LAKTKLKLGSNLVSLEVSAGNSSCPADGCEPAELLQVADASLYRSKEARRGTGSAILR
                     DSASAAGSG"
     gene            complement(4918..5850)
                     /locus_tag="VFD74_01745"
     CDS             complement(4918..5850)
                     /locus_tag="VFD74_01745"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZK48310.1"
                     /translation="MFVPRLRSVLPFLLVASTVAALIVLSGCTQADTSATTAATETTA
                     TTAGGGTTETTATTAGGGSGSTTATTGGAESVQPVSEAFKVCTECHSDFNQFLATSKV
                     LTKNFGHALHLNKGFKCEDCHAVPTHQPDKIAKPVMQACFVCHSQEPGGSAPGSCSTC
                     HPADFPLVPANHTAGGWLPGPNPGVVKVVSAKHAALAKEDRSYCDTCHAASFCDACHK
                     TPMPHAADWEATHPETVKTKGESSCTTCHPAQYLCNDCHHTGYKPGDTPWRLQHPPIV
                     KSAGAESCFKCHNPLTCEHCHITGELDMNLKPTG"
     gene            complement(5980..6972)
                     /locus_tag="VFD74_01750"
     CDS             complement(5980..6972)
                     /locus_tag="VFD74_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZK48311.1"
                     /translation="MMALHRDHEDGRGSPVLLMVVLLSFVAAIAAGLLLLAPWSSSAD
                     SSATSSTEASPDAQAPEELTGVTGGDTSDVSGAPEQVEGEPFRTCEVCHPDFLSKPDQ
                     TPANDLIFSHQTHLGNEVKCVTCHEPPLGHFDTPAPMMMACLQCHEGDTAPNECAKCH
                     RKIDEIAPGLDDPVVHLDPDIFTRESCAKCHDVATWCEQCHGVEMPHPSNWQDTHPTF
                     VSTRSDTCVKCHQSRDVKFCVTCHGVEMPHPAYWYSSHGDVARSNGDSCARCHPSAPQ
                     FCNQCHHAGFSPTSQWASRQHGSVVKDRGTAACFVCHEQAFCERCHVAGAFVKR"
     gene            complement(6969..>7681)
                     /locus_tag="VFD74_01755"
     CDS             complement(6969..>7681)
                     /locus_tag="VFD74_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48312.1"
                     /translation="TTQFVAPIADETCLSCHDDFLASREHEDKRQFSHDLHVAQRISC
                     STCHTAVGHSGVALPGPEACDACHGIQMPHPQGFGTSHGKAVVESGSDEVCRRCHNVY
                     LRCQTCHGVQMPHPEEWTGKHGEVAFPRLKVCSTCHQSSFCLTCHPVQMPHPDDWTTT
                     HGLPVLEKGSVVCSSCHEPDLCTACHGMPMPHPSDWGTSHPDTARQKRDECVLCHTEK
                     DCLACHVIHQTHGRGGGA"
BASE COUNT         1373 a         2493 c         2465 g         1350 t
ORIGIN      
        1 tgctcggctc gcccgtcgtc tccgtcgcct ccgtcatctc ggaatcccca cacggagccc
       61 tcgactcggc gtcccccgcc gcggctcctc agagtttcga gtcctgcgtg caacgcggct
      121 tcggactcct cgcgcgcggc gagcatgaag acggcgtggc cgagttccac gaggcgctcg
      181 ccctcatcga cgaccgagag atgaaggtga ggctccacat tgtgatcggc aacgcctatc
      241 gatcgcaacg catgtacgaa gccgccggcg ctgcgtacct acaggcgacc ttgcacaccg
      301 accatccgat gctgcttgag catctggagc ggattatcgc tgagatgatg gattcggacg
      361 acgtcggcgc cgccgacaca acgtccgagc cagagaagga ggagtgagta gcatggcgag
      421 gatggacacc gaccttctgg gtctgcagat cgtcgcgctc gacagcgcgg ccgtagtggg
      481 cgagatcgat ggcttgctca tcgatgacgc taccatgaaa gtagccgggt tccttgtgga
      541 cctgggcctg tacgaagcct ccgtcctgtc gttcggttcc gcgcacgcgg tcggcctgga
      601 cgcagtgatc gtcgaatcgg ccgacaagat caccgagatc tccgccgatc cgacgctcga
      661 ggctctcaac gagagggaca tcgcgatctc ggacgccaag gccatcaccc gttcaggccg
      721 cagcgtaggc actatcggcg acttctttgt cgacacggag aacggtgacg tggtggggat
      781 ggagttcctc gccgccgatc agacgatcta tccgcgcggc atcgccgtga tccctgcctc
      841 agtggtcttc cggctcggtc gcgacatcgt tgtgctagat gacgactacg accagcactt
      901 cctgaaggac ggcgactcgc tcgagagggt cgggcactcc cgttcggcgg agcccgcacc
      961 cgtggttacg cccgaagtgg tggtggcgcc cgaagcagcg acggaggctg aatctccggt
     1021 gctcgaagtc cccgaagaag ccgaagccgt ggccgtcgag ccggcggccg agtcggaact
     1081 cgagccccct gttgcaccgg ctgccgcgat cgagcccgag ccagccgccg acgctgacac
     1141 tgcgacggaa gttgagacac ccgccgcaga gaccacccca accgtgcccg ccgacgacct
     1201 gttcgccacg gagcacaagc acttcctcat cggcaagcgc gttctgcgcc gcatagagac
     1261 ccccgggggc gaggtcatcg ccgaggaggg agatatcgtg acgttcgaca ccatcgagaa
     1321 ggcgaagagc agcgaccaac tgctgatcct ctctctgaac gtggagtaga gccggacgcg
     1381 atcgggcggc tcaggccacc atggtgcggt acacctcgat ggtctccgcg gccgccctct
     1441 cccaaccgaa ccgcgctgac tgacacaacc cttcgcggcg aaggttgccg ttcagatcag
     1501 gctccgtgag gacccggtgg atgcagtcga cgaactgctc caccgacgtc gggttgaaca
     1561 tcatggccgc gcggccggtc acctcgggca acgccgagga gttggagacg acgcatggcg
     1621 tcccgcaggc catggcctcc agcggcggca ggccgaaccc ttcgtacaga gaggggaaca
     1681 cgaacacggc ggccgaagag tagacggccg ccaggtcgtc ttcgcgcacg aacccgggga
     1741 acacgacgcg atccttgaga ccgcggcgct ccgcttcatg cgcgatcttg cggaactccc
     1801 cttcctgcgc tccggccatg gccagggtga gcgggacatc gagcaccttc tggagcgccg
     1861 cccagccccc gaccatgaac tccaggttct tgttcgggcg gaagtcgccg atccacaggg
     1921 cgaaacgctc cggcagcccg tagcgccggc gcgccgaagc ccgttcggcc tcgttctcca
     1981 ccgggtggaa ctggctggac acgccgttgt ggatcacgcg cacgcgcgac gcttcgacgc
     2041 cggcgaaggc ggtgagggtg gcgagcgaga tctgcgacac ggtgatgatg cagcgggcgg
     2101 cgccgagcac ccgatccaac tggcgccgat agcgcactcg cgcaaccggg ttgcgaaccg
     2161 tgcgcgggta gatcagtggg atgacgtcat ggatggtcgc cacgacgggc acgcccacag
     2221 ggatgggagc gtagtggctg ggaacgtgca gtatatcgac gccgctctgc tcgaccatcc
     2281 gcgcgaacac ggcgcgaccc gaatgcgacc gagggtcgat gttggtggac accagcgaga
     2341 aacgatctcc ccgggggacc gactctcgct tctgatcggg gcagaagacc acgaactcga
     2401 cgtcgtcacc cgctaactcc cccttcccga actgctgaag aaggttgcgg gagtacgtgc
     2461 cgatgccgct ccaatcgatg gtccgagcgt cgaatccgat tctcaagcgt cgcctcgcct
     2521 gggtgctggt tcgtcggtgc cctgtcctcc agcctggatt ctaccagacg ctacctggct
     2581 cccggcgctc tcgctcgggg gcctcgacat cggtctcggg ggcctcggcc gcggcgatcg
     2641 tcctcttctc cgaatctcga cccgcgaccg ggaaggtgac cactctcgcg agcggcggtt
     2701 tgggggtgtg tcccacccag tcgatcaccc ctgagcgagc cagctcacac aggatgctga
     2761 gcgtctcggt gggagagaag ggcgacgtcg ccgccacttc catcggactc ttctgcccgt
     2821 cgacgtgcgc gagcacctgc actttgcgat acgggaggtc gcggatcgtg cccgtctcgt
     2881 caccgtgtgc cgccacccac agcgtccttc ccgtggatcc aaggatcctg cggagtctat
     2941 cgagttcgtc ggaggctgag gtgagctcga tcaggaagtc ggtgatcggg aacatcacgg
     3001 atgtctgcga caccggcccc aaatgcatct cgaatgtagc gtctgcctcg ttctcggcgg
     3061 cgtatagcag cgattgcatc atgagacgcc gaaggacgat ctcgacttca cgcttggtga
     3121 ggtagccctg ctcgaccacg agctggccca accgcttgcc cctcgtagtg cactcggtga
     3181 gcagacgttg ccgatcgacc gagttgatca ggccgaggga ggccaacaag tcacccagtt
     3241 gccacgcccg accgctgtcg gcgagtccgc tcagcgcgcc gtcatgcagg tacaccacca
     3301 tcgcccgttg gccgggaaag agcagcctga tagagcccat cttcctgaag agggccccca
     3361 cctgcagcag ttcagcgagg tgaagcgtct cgagtgagcc cgacgcagac atcagctggt
     3421 gtgttctccc gtttcgcccg tgcacaatga agacgccctc ggcgcctcca ctgggaccta
     3481 tcggcacgcc ccgcgactgc cttaagcgtt ggcggcggcg gatggcgagc caaagcgaac
     3541 gaccggccca tatggtggtg cgcgcagcgt acctcctggg cggcgtgata ccatcgttca
     3601 agcggcttcc gcacaccgat tcgcgcgctc gagcaagatg tttgcatccc tccccgacaa
     3661 ctactatagt actagagtgt agttttctac tcatggggga atcgaggcga cgtgtccgac
     3721 gggattgcgc cacaagggaa aacagtccgc agcgcgccta gccctctgtt ccagaaggtc
     3781 gaaggcgtcc tcgacgccgg caaggtccaa gtcaccaaga actggctcgc ccgaatcatc
     3841 gagcgcatcg acgacctggc ctcactggag cagttcccca cccaggagag catcaagacg
     3901 tcggtagaac tgatcgaggg tctcgccgcg gtcctcagtg acgaagacaa gctcagtggg
     3961 ttcgagccgg gcggcaagtt ctacgccctc gcagcaggac tcggaatcac cgatggtggc
     4021 ggcgctcgag gtctggtgac gctctcccag aacatgctgg agctcgagaa tgccatctgg
     4081 gctcttcttg ccggggcact ccgcaacgag gaccgggacc tactccgact cgtggcccgg
     4141 ctgcgggtgg cgatgcacgg catccacacc gcatccacag aggcctactt ccaccgctcc
     4201 aacaccgaat tggaccgcct cgcccacacc gaccaactca ccgggctcta caacaggcgc
     4261 tacatgatcc aggagttgga gcgccacggt gagatgtaca agcgctaccg gcatcccttc
     4321 tcagttctca tgctcgactt ggacaatctc aagactgtca acgatacgct cgggcacccc
     4381 gccggcgatg cagcgctcaa acacctggct atgctcctgc gcgtcaatat ccgtgacgtg
     4441 gacatcgcct gccggtacgg cggagacgag ttcgtggtgc tcatgcccga gaccgagcag
     4501 aacgtggtgc agatcgtcgg cgaccgcata gcggcctcac ttgccaagac caagttgaag
     4561 ttaggatcca atctggtcag cctggaggtc agcgccggca attccagttg cccggcggac
     4621 gggtgcgagc ccgccgaact gctccaggtg gccgatgcga gcctctaccg gtcgaaggag
     4681 gcccgcagag gcaccggctc cgcgatcctc cgcgactcgg cgtctgccgc cggcagcggc
     4741 tgactcgggc gatcttccgg ggggagccgg gcccgtcggg cgtcggctcc atccctacag
     4801 catcacctgg tccgatacga acaagtgggc ggcggggcgc gatttgcgcc ccgccgccca
     4861 cttacttgtt cacggcactg atcgtcgtcc gatcacccac tcttcagaaa agggcgatca
     4921 tcccgtcggt ttgagattca tgtccaactc acccgtgatg tgacagtgtt cgcaggtgag
     4981 cgggttgtga cacttgaaac acgactcggc gccggcgctc ttgacgatgg gcggatgctg
     5041 tagtctccaa ggagtgtcac cgggcttgta accggtgtgg tgacaatcgt tgcagagata
     5101 ctgcgccggg tggcacgtcg tgcacgaact ctcgcccttg gtcttgaccg tctcggggtg
     5161 agtggcctcc cagtcggccg cgtggggcat gggagtcttg tgacaggcgt cgcagaagct
     5221 ggcggcgtgg cacgtgtcgc agtacgatcg atcctctttc gccagcgctg cgtgtttcgc
     5281 tgagaccacc ttcacgacac ccgggttcgg gccgggaagc cagccgccgg ccgtatgatt
     5341 ggccggaacg agagggaagt cggcgggatg gcaggtggag cacgatcctg gcgctgaccc
     5401 tcctggttcc tggctgtggc agacgaagca ggcctgcatc acgggcttgg cgatcttgtc
     5461 gggctggtgc gtcggcaccg cgtgacagtc ctcacactta aaccccttgt tcaggtggag
     5521 tgcgtggccg aagttcttcg tcaggacctt gctggtcgcg aggaactggt tgaagtcgga
     5581 gtggcactcc gtgcagacct tgaaggcttc ggacaccggt tgcacggact ccgctccgcc
     5641 ggtggtggcc gtggtgctgc cgctccctcc gcccgctgtg gtcgcagtgg tctcagtcgt
     5701 ccctccgccc gcggtggtcg cggtcgtctc agtggcggcc gtggtcgcgc tggtgtcggc
     5761 ctgcgtgcac ccactgagca ctatcagtgc ggcaaccgtc gaggccacca ggagaaatgg
     5821 caggacactc ctcaacctgg gtacgaacat cggctcacct ccttcccatc tgttgtcgtt
     5881 gcccgagttt gtcgagccga ttctctacta tttcgccgca ccacgcaagt cgcccgtcac
     5941 ttacggcccg tttcccacga gcagccgcgc ggagcgcttc taccgcttca cgaaggcgcc
     6001 cgcgacgtga caacgctcac agaaggcctg ctcgtgacaa acgaagcacg ccgccgtgcc
     6061 gcggtccttg accaccgacc cgtgctgccg tgaagcccac tgagacgtag gagagaagcc
     6121 ggcatggtga cactggttgc agaactgcgg agccgaaggg tgacagcgtg cacaggagtc
     6181 accgttgctc cgcgccacgt cgccatggct cgaataccaa tacgccgggt gcggcatctc
     6241 cacgccgtgg cacgtcacgc agaacttcac gtcacgcgat tggtggcact tcacgcacgt
     6301 gtcggaacgt gtggaaacga aggtggggtg cgtatcttgc cagttggagg ggtgcggcat
     6361 ctccaccccg tggcactgtt cgcaccacgt ggcgacgtcg tgacacttcg cgcaactctc
     6421 tcgcgtgaag atgtcgggat cgaggtgaac gaccggatcg tcgaggcctg gggcgatctc
     6481 gtcgatcttg cggtggcact tggcgcactc gttcggagcg gtatctccct cgtgacactg
     6541 caggcaggcc atcatcatcg gtgcgggtgt gtcgaagtgc cccaggggag gctcgtggca
     6601 cgtgacgcac ttcacttcgt tgccgaggtg ggtctggtgg gagaagatca ggtcgttcgc
     6661 gggcgtctgg tccggcttgc tcaggaagtc cgggtggcac acctcacagg tgcggaaggg
     6721 ctcgccctcg acctgctccg gagccccgga gacatcgctc gtatcccctc ccgtgacacc
     6781 ggtgagctcc tctggggcct gcgcatcggg agaagcttcg gtcgagctcg tggctgaaga
     6841 gtcggcggag ctcgaccacg gtgcgagtag gagcagccca gccgcgatcg cagccacgaa
     6901 actgaggagc accaccatca gcaacacggg tgagccgcgc ccgtcttcgt gatcccggtg
     6961 aagggccatc acgcgccacc tcctctaccg tgcgtctggt gtatcacgtg gcacgcgagg
     7021 caatccttct cggtgtgaca caacacgcac tcgtcacgct tctgacgcgc tgtgtcgggg
     7081 tgggaggtgc cccagtccga ggggtgcggc atgggcatcc catgacaggc cgtgcacagg
     7141 tcgggctcgt gacacgagga gcacacgacc gatcccttct cgagcacagg gaggccgtgg
     7201 gtcgtggtcc aatcgtcggg atgaggcatc tgcaccggat gacacgtgag gcagaaggaa
     7261 ctctggtgac atgtggagca caccttcagc cgcgggaacg ctacctcgcc gtgcttgccg
     7321 gtccattctt ccgggtgcgg catctgcacc ccgtgacagg tctggcaacg aaggtagacg
     7381 ttgtggcacc tgcggcagac ttcgtccgag cccgactcga ccaccgcctt cccgtgcgat
     7441 gtcccgaagc cctgagggtg aggcatctgg atgccgtggc aggcgtcgca cgcctcgggg
     7501 cccggcagag ctacgccgct gtggcccacg gccgtgtgac acgtcgagca cgagatcctc
     7561 tgtgcaacgt gcagatcgtg cgagaactgt ctcttgtcct cgtgctctcg actcgctagg
     7621 aagtcgtcgt ggcaggacag acaggtctcg tcggcgatcg gcgcgacaaa ctgcgtggta
     7681 g
//
LOCUS       DASRMJ010000060        10322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_440757, whole genome shotgun sequence.
ACCESSION   DASRMJ010000060 DASRMJ010000000
VERSION     DASRMJ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440757"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFD74_01760"
     CDS             complement(<1..346)
                     /locus_tag="VFD74_01760"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZK48313.1"
                     /translation="MFVVIGERINTSRPAVRAATEDRNAEYIRDDVRKQQAAGATYID
                     VNAGARIGHEAEDLKWLLEVIQPVIDIPICLDSPDHAVLETAYGMVARPPMINSISLE
                     RERYDSMLPFLAG"
     gene            complement(337..978)
                     /locus_tag="VFD74_01765"
     CDS             complement(337..978)
                     /locus_tag="VFD74_01765"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HZK48314.1"
                     /translation="MEDTLTGLSMSDFEARVASIDATPGGGSVSALAGSLGSALGAMV
                     WRLTQAKTSSDLSEERLDGLIAELDELGAALRAKVDEDAASYDGVITAIRLPKTTDAE
                     KESRRAAIVAATRVATEVPLETARLCVEVMENCLVAARLGHAAAVTDAGVGLLAAFAG
                     VKGALYNVRVNLAGASAEGRDDAMLDEVASLGVRAEAMLREGDALVGERLSCS"
     gene            complement(1045..2322)
                     /gene="hutI"
                     /locus_tag="VFD74_01770"
     CDS             complement(1045..2322)
                     /gene="hutI"
                     /locus_tag="VFD74_01770"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZK48315.1"
                     /translation="MSRDTHAAAEDLNAAPGRGRPADLVVRNAGELLTCADGLGVISG
                     GAVAVGEGRISWVGPDPDLEAAVEIGPRTRVIDAGGRVVMPGVVECHTHVVFGGDRAA
                     EFQMRVAGKPYQEIAAAGGGIKSTVNATRAASRDELFERGRRHLGWLLRYGVTTVEVK
                     SGYGLSVHDELRILEVYRDLAAVAPQTIVPTLLGAHTVPLEFAGRADRYVDLVVGEMV
                     PRAAEAGLARFCDVFVEEGAFTLEQARRVLEAGLAHGLRPKLHADQLSAGGGAELAAE
                     LGGASADHLDHVSNAGIAALAAAGVTAVLLPGAVFFLGLHEYAPARRLLEAGVRVALS
                     TDFNPGTCYTENVFLMGTIASAHMKMSAAEVILALTLNAAHAIDMAGEIGSLEVGKRA
                     DILVLEAESHLTIPYHWGINPVATVIKDGVVVQ"
     gene            complement(2319..3335)
                     /locus_tag="VFD74_01775"
     CDS             complement(2319..3335)
                     /locus_tag="VFD74_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK48316.1"
                     /translation="MEHFGAPEFLGPYLGRFFEEADTALVHSLRTGPLTPDELCGRLP
                     QMDAAGLERAHRRGMVDHTPGGLVTLADFHARFEIWAIFEGWKDIPADMRAALNRWAQ
                     DAYLERKRAQIEDLQAGREPESHLENSEYLLLHEAITLLDRAERIYLWPCNCRSMMGA
                     CSKPVNTCIRFSNERGVGWEISRERAREIVREANRAGLMQTGEVAELEGAVIGAICNC
                     CTDCCYPHLVSARLGAEKLWPRSRYLARLLPGLCTACGRCARRCPFGAFVASTRESAD
                     TGAARLEITFVEDLCRGCGVCATGCPEDAIAMDPLPATGPGAPSDSSLRAVPAALSTP
                     EAAR"
     gene            complement(3419..4789)
                     /locus_tag="VFD74_01780"
     CDS             complement(3419..4789)
                     /locus_tag="VFD74_01780"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZK48317.1"
                     /translation="MRSEDVRKVHEAGVGILERTGVVLQDADTVRLFADRGFPVDGQR
                     VRISGERVEWAAKAAPRTFELHARGARENLRFGERMVVSGTGGAPFILEGSTLRVGTL
                     TDLMAIAKVMHLSSNVDMLGFAVDPQDVPVEKRARRTLHALLTLSDKPPTLLGFAPDL
                     ADAAMTTAEIIWGARWHERPCLLAVLNSTSPLILDAEACWGIRTLARMGQPLSVTPCV
                     MGGATGPATMAGILALQHAETLAGLVLTQLVREGSPYVYGGTSSVTSMVSGDLLVGVP
                     QYWTMMAATVQLGHHLGLPVRAGGGLTDSHLPDMQAGIEAAMGLAAVARCGVDYVHHG
                     SGIMSSFNAVSFEKFIIDDELVGMIRARNLPIVVDEETLTLDVIDSVGPGGNFLLEAH
                     TAGHCRDYERPTFFNRRRHDVWLSRGGWDLATAARERFTKLLEAYREPELDPLTLRQL
                     AECCLL"
     gene            complement(4828..6375)
                     /gene="hutH"
                     /locus_tag="VFD74_01785"
     CDS             complement(4828..6375)
                     /gene="hutH"
                     /locus_tag="VFD74_01785"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZK48318.1"
                     /translation="MSIVIIDGDSLTLEQARAVAHEGAEVGVAPAARRRIDEARAYVD
                     RIIAEERVVYGITTGFGKFSDVTIAGSDVLELQRNLILSHCCGVGEPLDAEKTGALML
                     LRANVLAKGYSGARYVVLETLVDMLNRGVLPVIPTRGSVGASGDLAPLAHLAAVIIGE
                     GEAVWEGRRLSGREAMAAAGIAPLTLQAKEGLALINGTQFMTGIGLLALFKAETIAKC
                     ADVACAMTLEALRGTDAAYGPLTHEVRPHPGQVATAANLMRLLDGSEILPAHADPSND
                     NKVQDAYSLRCAPQVHGAGKDALAYVRRVLEVEVNAATDNPLVFPEQDRVISGGNFHG
                     QPISQAMDLLSIALSYTAGISERRIEYMLDPATSDHLPAFLTKWGGLHSGLMIAQVTA
                     AALVSENKTLAHPASVDSIPTSANKEDYVSMGAYAARKADDVCQNALTVLAVEFLCAA
                     QALEFGGDLRPGRGVAAARRVIREHVAPLERDRPQTPDIESIAKLILDGDILRAVEDA
                     VGPLEMA"
     gene            complement(6386..7837)
                     /locus_tag="VFD74_01790"
     CDS             complement(6386..7837)
                     /locus_tag="VFD74_01790"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZK48319.1"
                     /translation="MKDLRNASLVGRLSFLSEGDKRDIYLSALEIMSKVGMVVYHEGA
                     RRMLLEAGCVLLAESRVLMPRHLVETARLSVPSQVDVYDRDGELAMELGGYNSYFGTG
                     SDLMSTYDLDTGEHRPSTLEDVARAARLCDALPNMDFIMSSAHPTDRNPHHSYLLSFQ
                     AMVKNSRKPLVMTAENAGDLQVMIDVARALRGGVEELRRRPYFVVYNEPISPLEHPVE
                     SIEKLLLCADSGVPSIYSPAPLAGATAPITVAGHTTQGVAECLFGLVIHQLHKPGAPF
                     LFGIGPAVLDLVTAQSSYNAVEYLMTYMCAVEMAKWLDIPNWGYAGTSDSHLLDAQAG
                     MEATQLTFLAMQAGSNLSHDVGYLDFGLTASLEEIVVVDEFISMNRRLLRGIPVDRDT
                     LAVEAVAEIGPGGHFMTSDHTYRHMREVQWRPTILNRHGRDRWLADGSLDLAAKARRK
                     AQNILDTHEVPALAPHVAAALGKSIEAFTSTEG"
     gene            complement(7892..9409)
                     /locus_tag="VFD74_01795"
     CDS             complement(7892..9409)
                     /locus_tag="VFD74_01795"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZK48320.1"
                     /translation="MRAPPSRWRRSWGRCRGRHRPTTREHLLMKNPLIAQRGVRFQKL
                     SVGQCTAIHDASCRLLETVGIEAHHEQAREILKKAGAVVEGTRVRVPRRLVDWALGVV
                     PRSFTLHTRVGVAAMEVGGANVYFGNGSETPNVLDHRTGERRHGTLADVVEGVRLLDA
                     LPNIDFVMSLFVPWELAPQVAYVEQFKAMIENSTKPFLFLSPSGEDFRAMVEMLEAVA
                     GGEEALREKPRGLGYINVTHPFRHEFDDLDKLMFLARKGVPYTYNPTVLRGASGPITQ
                     AGSMAVGNMGELFGLVLAQLVNEGCPATMSGGTADKLDMRSMIDIYSAPENRVAFTEM
                     ARFYDIPHFGLAGGSDAKLVDEQAAAEASLTMLVEALAGSNLIHDVGYMESGMSNSLA
                     QIVICDEIIGWIRRFMDPIVVDEETLALGLIERVAPMGDYLAEDHTLAHFGEDWYPGL
                     FDQSSHDKWVKAGSLRLGEKATRRVDELLAHHQVEDLPSDVVAQLDRIIAARAAQ"
     gene            complement(9363..9998)
                     /locus_tag="VFD74_01800"
     CDS             complement(9363..9998)
                     /locus_tag="VFD74_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HZK48321.1"
                     /translation="MSSYQQIQQDIEQGKKDLVLAQVQQALDEGLPAHEILQEGLIAA
                     MDIVGAKMEREEMFIPEVLRAAKTMEAGLDLLRPHLAVGDLKVSGTVVIGTVKGDLHD
                     IGKNLVAMLLASAGFRVVDAGINVTPDAFVEKVKESGADFLGMSALLTTTMPQMQETL
                     KALNAAGLRAQVRVLVGGAPVTQSWADEIGADGYASDAGAAIKVAKELGAL"
BASE COUNT         1690 a         3481 c         3435 g         1716 t
ORIGIN      
        1 tccccgccaa gaacgggagc atggagtcgt agcgctcgcg ctccaggctg atcgagttga
       61 tcatcggggg cctcgcgacc atgccgtagg ccgtttcgag gacggcgtgg tcggggctgt
      121 ccagacagat ggggatatcg atcacgggct ggatcacctc gaggagccac ttcaggtcct
      181 cggcttcgtg accgatacgc gcgccggcgt tcacgtcgat gtaggtggcg ccggccgcct
      241 gctgcttcct tacatcgtct cggatgtact cggcgttgcg atcctccgtc gcggcccgca
      301 ctgccggcct tgaggtgttg atgcgttctc cgatcactac gaacatgaca atctctcccc
      361 caccaaggcg tcgccttcac gcagcatggc ctcggccctt acaccgagtg acgccacttc
      421 atccaacatt gcgtcgtcgc ggccctctgc ggacgcgccg gcgaggttca cccgcacgtt
      481 gtagagtgcc cccttcacgc cggcgaaggc cgccaggaga ccgacgccgg cgtcggtgac
      541 cgccgcggcg tggcccagac gcgccgccac gagacagttc tccatcacct cgacacagag
      601 ccgcgccgtc tccaacggca cctcggtggc gacccgtgtt gctgcgacga tggcggcgcg
      661 gcgggactcc ttctcggcat cggtggtctt aggaagacgt atggctgtga tcaccccatc
      721 gtagctcgcg gcatcctcgt ccaccttggc ccggagcgcc gcgcccagtt cgtcgagttc
      781 cgcgatcagc ccatcgagcc gctcctcaga cagatcgctc gacgtcttgg cctgggtgag
      841 tcgccatacc atcgcaccga gcgccgaacc gagactcccg gccagggccg agacgctgcc
      901 ccctccgggc gtggcgtcga tcgacgccac gcgggcctcg aagtcgctca tcgacaaacc
      961 ggtcagtgtg tcttccacga acgatcctcc atcgatagat actctgtaca actaccgtgc
     1021 gaccagtgca cgactgctgt acgactactg tacgaccacg ccgtccttga tcaccgtcgc
     1081 aaccgggttg atcccccagt gataggggat ggtcaggtga ctctcggctt cgagcacgag
     1141 gatgtcggcg cgcttgccca cctcgaggct gcctatctcg cccgccatgt cgatcgcatg
     1201 ggcggcgttc agggtgaggg cgaggatcac ttccgccgcg ctcatcttca tgtgggcgga
     1261 cgcgatcgtg cccatcagga acacgttctc cgtgtagcag gtgcctggat tgaagtcggt
     1321 ggagagggcg acgcgcacac cggcttccaa tagccggcgc gccggagcgt actcgtggag
     1381 cccgaggaag aagacagccc ccggcaggag caccgcggtg accccggcgg cggccagcgc
     1441 ggcgataccc gcgttgctga cgtgatcgag gtggtcggcg gaggcgccgc cgagctcggc
     1501 cgcgagctcg gcgccgcctc ccgccgaaag ctggtcggcg tggagtttgg gtcgcagccc
     1561 gtgcgccaag ccggcctcga ggacccgccg ggcctgctcc agggtgaagg cgccctcctc
     1621 cacgaacaca tcgcagaaac gggcgaggcc cgcctccgcc gcccggggca ccatctctcc
     1681 caccacaagg tccacgtagc gatcggcccg cccggcgaac tcgagcggca cggtgtgggc
     1741 gcccagaagg gtggggacga tcgtctgggg cgctaccgca gccaggtccc ggtagacctc
     1801 gaggatgcgg agttcgtcgt gcacagagag accgtagccc gacttcactt ccaccgtggt
     1861 cacgccgtag cgcagcagcc accctaggtg gcgccgcccg cgctcgaaga gctcgtcgcg
     1921 cgaagccgcc ctcgtcgcgt tcaccgtgct cttgatgccg ccaccagcgg cggcgatctc
     1981 ctggtagggc ttgccggcca cccgcatctg gaactcagcg gcccgatcgc cgccgaagac
     2041 cacatgcgtg tggcactcca ccactccggg catgaccacg cggccgcccg cgtcgatcac
     2101 tcgagtgcgg gggccgatct ccacggcggc ttccaagtcc gggtcgggtc ccacccagga
     2161 gatacggcct tcgccgaccg cgacggcgcc ccccgagatg acgcccagtc cgtcagcgca
     2221 ggtgagaagc tcgcccgcat tccgcacgac gagatcggcg ggccggccgc ggccaggcgc
     2281 ggcattgagg tcttcggccg cggcatgggt atcgcgactc atcgcgcggc ctcgggagtc
     2341 gagagcgcgg ccggaaccgc gcgcagcgat gagtccgaag gcgctcccgg ccccgtggcc
     2401 gggagcgggt ccatcgcgat ggcgtcctcg gggcagccgg tggcgcacac gccacatccc
     2461 cggcagaggt cttcgacaaa ggtgatctca agtcgggcgg cgccggtgtc cgcagactcc
     2521 cgcgtgctcg ccacgaacgc cccgaaagga cagcgccggg cacaacgtcc gcaggccgtg
     2581 cacagtccag gcaatagacg agccaggtac cggctccggg gccagagctt ctccgccccg
     2641 aggcgagcgc tgacgaggtg agggtagcag cagtcggtgc agcagttgca gatggcgccg
     2701 ataacggcgc cctccagctc cgccacctcg ccggtctgca tcaggccggc tctgttcgcc
     2761 tcgcggacga tctcccgcgc ccgctcccgc gagatctccc accccacccc ccgctcgttg
     2821 ctgaagcgga tacaggtgtt caccggcttg gagcaggccc ccatcatgct gcgacagttg
     2881 cagggccaca gatagatgcg ctccgcacga tccagaagag tgatcgcctc gtgcaacagt
     2941 agatactcgg agttctcgag atgagactcg ggctcccgcc cggcctgcag gtcctcgatc
     3001 tgcgcgcgct tgcgctcgag ataggcgtct tgcgcccagc gattcagcgc cgcgcgcatg
     3061 tcggccggta tgtccttcca tccctcgaag atcgcccata tctcgaagcg ggcgtggaag
     3121 tcggccaggg tgacgagacc tcccggcgtg tggtccacca tcccgcgacg atgggcccgc
     3181 tcaaggccgg cggcgtccat ctgcggcagc cgcccacaga gctcgtcggg agtcagcgga
     3241 ccggttctca gcgagtgcac caaggccgtg tcggcctcct cgaagaaccg gcccagatag
     3301 gggccgagga actccggagc gccgaagtgt tccacgaagc atccggggtc ggacaagggt
     3361 ggaccggcca gggttggttc ctgtgcggag ggcgcgtgca tgtaagtgtc ctggttcgtc
     3421 acaacaggca acactcggcc agttgtcgca gggtcagcgg atcgagttcg ggctccctgt
     3481 acgcctccag taacttggtg aaccgctctc gcgcggccgt cgccaggtcc cagccgcctc
     3541 gactcagcca tacgtcgtga cggcggcggt tgaagaaggt gggacgctcg tagtcgcgac
     3601 agtggccggc cgtgtgggcc tcgaggagga agttgccgcc ggggcccacg ctgtcgatga
     3661 cgtcgagggt cagcgtctcc tcatccacca ctatgggcag attccgggct cggatcatgc
     3721 ccacgagttc gtcgtcgatg atgaacttct cgaacgagac ggcgttgaac gagctcatga
     3781 tccccgaccc gtggtggaca tagtccacgc cacagcgggc caccgcggcc aaccccatgg
     3841 cggcctcgat acctgcctgc atgtcgggca agtgggaatc ggtgaggcct cccccagcgc
     3901 gcacgggaag cccgaggtga tggcccagct gcacggttgc ggccatcatg gtccagtact
     3961 gcggcacgcc caccagcaag tcacccgata ccatggacgt caccgatgac gtgccgccgt
     4021 acacgtaggg actcccctcg cgcacgagtt gggtgagcac cagtccggcc agggtctcag
     4081 cgtgctggag tgcgaggata ccggccatgg tggccggacc ggtggcgccg cccatgacgc
     4141 acggcgtgac ggagagcggc tggcccatcc gggccagcgt ccggatcccc cagcacgcct
     4201 ccgcatcgag gatcagcggc gaggtgctgt tgagcaccgc caagagacag ggccgctcgt
     4261 gccaccgcgc tccccatatg atctcggccg tggtcatcgc cgcatccgcc aggtccggtg
     4321 cgaaaccgag gagcgtcggc ggcttgtcac tcagcgtgag tagggcgtgg agcgtccgtc
     4381 gggcccgctt ctcaacggga acgtcctgcg gatcgacggc gaaaccgagc atgtcgacat
     4441 tcgacgacag gtgcatcact ttggcgatgg ccatcagatc ggtaagggtg cccacccgca
     4501 gggtggagcc ctccaggatg aagggcgcgc ctccagtgcc tgagacaacc atcctctctc
     4561 cgaagcgcag gttctcccgg gctccgcggg cgtgcaactc gaaggttcgc ggagccgcct
     4621 tcgccgccca ctccaccctc tcgcccgata tgcggacccg ttgtccgtcc accgggaacc
     4681 cgcgatcggc gaacagtctc acggtgtcgg catcctgcag gaccacgccg gtcctctcga
     4741 ggatgcccac cccggcttcg tgcaccttcc tgacgtcctc tgagcgcata gcctcaccca
     4801 tctccgaggg ccgaggatca cccacggcta cgccatctcc agcggaccga ccgcgtcctc
     4861 gaccgcccgc aagatgtccc cgtcgaggat cagtttcgcg atgctctcga tgtcgggtgt
     4921 ctgagggcgg tcccgctcga gcggggccac gtgctcgcgg atgacccggc gggcggcggc
     4981 gaccccccgc cccggccgaa ggtcgccgcc gaactccagc gcctgcgccg cacacaagaa
     5041 ctcgacagcc aagaccgtca gcgcgttctg gcacacgtcg tcggccttgc gggcggcgta
     5101 ggcgcccatg ctgacgtagt cctctttgtt ggcggaggtg gggatggagt ccaccgaagc
     5161 cgggtgggcc agggtcttgt tctccgagac gagtgcggcg gcggtcacct gggcgatcat
     5221 gagcccggag tgaagcccgc cccatttcgt gaggaaggcg ggcagatggt cggaggtggc
     5281 cgggtcgagc atgtactcga tgcgccgttc gctgatgcct gcggtatagc tgagggcgat
     5341 gctgaggaga tccatggcct gcgaaatcgg ctgaccgtgg aagttgccgc cggatatcac
     5401 gcggtcctgc tcggggaaca ccagcggatt gtcggtggcc gcattcacct ccacttcgag
     5461 cacccgtcgc acgtaggcca gggcatcctt gccggcgccg tgcacctggg gcgcacaccg
     5521 cagggagtag gcgtcctgca ccttgttgtc gttggacgga tcggcgtggg cgggcaggat
     5581 ctcgctgccg tcgagcagac gcatcaggtt ggcggcggtg gcgacttggc ccggatgcgg
     5641 acgcacctca tgggtgagag gcccgtaggc ggcgtcggtg ccgcggagcg cctcgagagt
     5701 catagcgcag gccacgtcgg cgcacttggc gatggtctcg gccttgaaga gagccagcaa
     5761 tccgatcccg gtcatgaact gggtgccgtt gatgagggcc agaccctcct tggcttggag
     5821 cgtgagcggc gcgattcccg ccgcggccat ggcctcccga ccggagaggc gccgcccttc
     5881 ccacacggct tcgccttcac cgatgatgac agccgcgagg tgggccaaag gagcgagatc
     5941 gccggaggcg ccgacggagc cgcgggtggg gatcaccggg aggactccgc gattgagcat
     6001 atccaccagg gtctcgagaa ccacgtagcg tgcacccgag taccccttgg ccaacacgtt
     6061 cgcccgcagg agcatgagag ccccggtctt ctctgcgtcc agcggctcac ccaccccgca
     6121 gcagtgactc aggatgaggt tccgctggag ttccagcacg tcggagcccg ctatggtgac
     6181 gtcgctgaac ttgccgaaac ctgtggtgat gccgtagacc accctctctt cggcgatgat
     6241 gcggtcgacg tacgcccggg cctcgtcgat cctgcgccgg gcggccggcg cgacacccac
     6301 ctctgcgccc tcgtgggcga cggcgcgggc ctgctccaaa gtcagactgt cgccatctat
     6361 gatcacgatg ctcatgcgga cccgcctacc cttccgtcga ggtgaaggcc tcgatcgact
     6421 tccccagcgc ggcagccacg tggggggcga gtgccggcac ctcatgcgtg tccaggatgt
     6481 tctgagcctt acggcgcgcc ttggcggcca gatccaggga gccgtcggcc agccaacgat
     6541 cgcggccgtg ccggttcagt atcgtggggc gccactgcac ctcgcgcatg tgccggtagg
     6601 tgtggtcgct ggtcatgaag tggccgcccg gcccgatctc ggctacggct tccacggcca
     6661 gagtgtcgcg gtccaccggg atgccccgga gcaggcggcg gttcatgctg atgaactcgt
     6721 caaccacgac gatctcctcg agcgaggcgg tgagaccgaa gtcgaggtag cccacgtcgt
     6781 gcgacaggtt cgagccggcc tgcatcgcca ggaaggtcaa ctgcgtggcc tccatcccgg
     6841 cctgcgcgtc aaggaggtga ctgtcgctgg tgccggcgta gccccaattg gggatgtcga
     6901 gccacttcgc catctcgacc gcgcacatgt aggtcatgag gtattcgacg gcgttgtagc
     6961 tcgactgcgc ggtcaccagg tcgagcacgg cgggaccgat gccgaacagg aacggcgccc
     7021 ccggcttgtg cagttggtgt atcaccagcc cgaagagaca ctcggcgacg ccctgagtgg
     7081 tgtgtccggc caccgtgatg ggggcggtgg ctccggccaa gggcgccggc gagtagatgg
     7141 aggggacacc gctgtccgcg cacaggagaa gcttctcgat gctctccacc gggtgctcga
     7201 gcggcgatat gggctcgttg tacacgacga agtagggcct gcgtcgcagc tcctcgaccc
     7261 cgccgcgcag cgcgcgggcc acatcgatca tcacctggag gtcgccggcg ttctcggccg
     7321 tcatgaccag cggcttgcgg ctgttcttca ccatcgcctg gaagctcagc aggtaggagt
     7381 gatgagggtt ccggtcggtg gggtgcgccg aactcattat gaagtccatg ttcggcaggg
     7441 cgtcgcacag acgggcggcc cgcgccacgt cttcgagcgt gctgggacga tgctcccccg
     7501 tgtcgaggtc gtaggtggac atgaggtcgg agcccgtgcc gaagtagctg ttgtagccgc
     7561 ctagctccat ggccaactcg ccgtcgcgat cgtagacgtc cacctgactg ggcacagaga
     7621 gacgcgccgt ctccacgaga tggcggggca tcaggacccg gctctcagcc aagaggacgc
     7681 aaccggcttc gagcaacatc ctcctggcgc cctcgtggta gacgaccatg cccaccttgg
     7741 acatgatctc cagggcggag aggtagatgt cgcgtttgtc accctcgctg aggaaggaga
     7801 ggcggccgac aagagaggcg ttccgtaggt ctttcacgac tggcagctcc aattcatgtc
     7861 tggattccgt accggcgcga acgccgggat ctcactgtgc cgcccgcgcg gcgatgatgc
     7921 ggtcgagctg tgcgaccacg tccgagggaa gatcctccac ctggtggtgt gcaagaagct
     7981 catccacccg gcgggtcgcc ttctccccga gacgcaggga tccggccttg acccacttgt
     8041 cgtggctgga ttggtcgaag agccctggat accagtcttc gccgaagtgg gccagggtgt
     8101 ggtcctcggc caggtagtca cccatagggg cgactcgttc gatgaggccg agggccaggg
     8161 tctcttcgtc gacgacgatc gggtccatga accgtcggat ccagccgatg atctcgtcgc
     8221 agatgacgat ctgggccagg gagttgctca tgcccgactc catgtagccc acgtcgtgga
     8281 tgaggttgct cccggcgaga gcttccacca gcatcgtgag cgaggcttca gcggccgctt
     8341 gctcgtcgac aagcttggcg tctgagcccc cggccaggcc gaagtggggg atgtcgtaga
     8401 aacgcgccat ctcggtgaac gcgacccggt tctcgggcgc cgagtagata tcgatcatgc
     8461 tgcgcatgtc gagcttgtcg gccgtgccgc ccgacatggt ggccggacac ccctcgttca
     8521 ccagttgagc caggacgaga ccgaagagct cgcccatgtt ccccacggcc atgctgccgg
     8581 cctgggtgat gggcccgctc gccccgcgaa gaacggtggg gttgtaggtg tagggcacgc
     8641 ccttccgggc caggaacatg agcttgtcga ggtcatcgaa ctcatgacgg aaggggtgcg
     8701 tcacgttgat gtagccgagc ccgcgcggct tctccctgag ggcttcctcc ccgccggcga
     8761 ccgcctcgag catctccacc atcgcgcgga agtcttcgcc gctggggctg aggaagagga
     8821 agggcttggt tgagttctcg atcatcgcct tgaactgctc cacatacgcc acctgcggcg
     8881 caagttccca aggcacaaac aacgacatga cgaagtcgat gttggggagg gcgtccagca
     8941 gacgtacacc ctccaccacg tcggcgaggg tgccgtggcg tcgctccccc gtgcggtggt
     9001 cgagcacgtt cggggtctcc gagccgttgc cgaagtagac gttggcgccg ccgacctcca
     9061 tggcggcgac gccgacccgg gtgtgaagtg tgaagcttcg tgggacgacg ccgagcgccc
     9121 agtccaccag tcggcggggc acgcgcacgc gcgtgccctc gaccacggcg cctgccttct
     9181 tgagaatctc ccgggcctgc tcgtgatggg cctcgatgcc cacggtctcg agcagacgac
     9241 agctcgcgtc gtggatggcc gtgcactggc ccacgctgag tttctggaaa cgtacaccgc
     9301 gttgagcgat gagtgggttc ttcatgagga ggtgctccct cgtagtcggt cggtgtcggc
     9361 ccctacagcg cccccaactc cttcgccacc ttgatggcgg cgcccgcatc ggaggcgtag
     9421 ccgtcggcac cgatctcgtc ggcccaggat tgggtgacgg gggcgccgcc caccagcacg
     9481 cgcacctgcg cgcgcagacc ggcagcgttc agagccttca gggtctcctg catctggggc
     9541 atggtggtgg tgaggagggc gctcatgccg aggaagtcgg cacccgactc cttcaccttc
     9601 tccacgaacg cgtccggggt aacgttgata ccggcgtcca ccacccggaa cccggcagat
     9661 gcgaggagca tggccaccag gttcttgccg atgtcgtgca agtcgccctt gacggtgcca
     9721 attaccaccg tgcccgagac cttcaggtcg ccgacggcca ggtgaggtct gaggaggtcg
     9781 aggccggctt ccatggtctt ggcggcacgc aggacctcgg ggatgaacat ctcctctcgt
     9841 tccatcttgg ccccgacgat gtccatggcg gcgatgaggc cttcctggag gatctcgtgg
     9901 gcggggagac cttcgtccag ggcctgctgc acctgggcga ggacgaggtc cttcttgcct
     9961 tgctcgatgt cttgctggat ctgctggtat gaggacacgt gctggctcct ggggtgacgc
    10021 ggttggatag tccaatgggg gccgacggga cgaccatcga gtcggtccac ccgtcggtcg
    10081 aagcgccggc ctgcggccgg ccgcgatgct acatcgccga gagaggctcc catgtccaac
    10141 acgcacacat acgatcccag cgcataggcc tgccgaaagg tacgctcggc gccaggcggc
    10201 ggtcgactga actccgcacc cgccatcgca tcccccgccg cagatccgcg ccggttcgcc
    10261 gcagttggga tgccaaggcc gcggtggtcg cagacatcat ccccaccccc ccgctcccaa
    10321 gc
//
LOCUS       DASRMJ010000061         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_80206, whole genome shotgun sequence.
ACCESSION   DASRMJ010000061 DASRMJ010000000
VERSION     DASRMJ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80206"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFD74_01805"
     CDS             complement(<1..326)
                     /locus_tag="VFD74_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HZK48322.1"
                     /translation="MARILVEGDDTLLGGLRDAAQPAFVCTIASSNTGAIEGISIAGA
                     SAEMRKYTPPADMEALFYGSARCIPGVAATPDGIPSPVIITMAALGAMALPVFVVDAG
                     SEVSPQT"
     gene            complement(389..1090)
                     /gene="cobU"
                     /locus_tag="VFD74_01810"
     CDS             complement(389..1090)
                     /gene="cobU"
                     /locus_tag="VFD74_01810"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HZK48323.1"
                     /translation="MPDVLFDGKGWHAPSLRSMILVTGGARSGKSTLAERLATDFGGR
                     VLYVATAEPRDEEMRLRIERHQAARSPEWGTLVAPRDAVERLREVEGDWDTLLLDCLT
                     LYTTTLLLEEEAGSSDPEVALAQVMQDLADYLSDHWPQAVVVTNEVGTGIVPGDRLSR
                     LFRDLQGRVNQAFARRADEVYLCVSGIPIKIKGQGSLQPAGEQSTRPADTRVDGSPWP
                     SDPPVGGSSSPRPAS"
     regulatory      complement(1083..1258)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1402..>2097)
                     /locus_tag="VFD74_01815"
     CDS             complement(1402..>2097)
                     /locus_tag="VFD74_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK48324.1"
                     /translation="RVAGGAGVVKVILTGSVDLERGVAPPAHFHSEELRRMVETAHEL
                     GVAVAVHANGAQAVTMAAEAGVDSVEHGILIDEESVRAMVDHDVVWVPTLTPLHALAV
                     SGRWAAVPRIIAAHEAMVAAGHAAGVRIAAGTDAGSPGVAHGAVLSEVAHLRSAGLSP
                     EAARAAVGAVAADLLGLGGGYGRLSRGAATDLVWFKQDPFADPSPDNARAPRPLGVLS
                     SPRPTASVGARMR"
BASE COUNT          348 a          734 c          698 g          317 t
ORIGIN      
        1 gtctggggag acacttcgct acccgcgtct accacgaaga cgggcagggc catggccccg
       61 agggccgcca tggtgatgat caccggcgag gggatgccgt cgggagtcgc ggccacgccc
      121 gggatgcacc gggcactccc gtagaagagc gcctccatgt ccgcaggcgg cgtgtacttg
      181 cgcatctcgg ccgaagcgcc ggcaatggag atgccttcga tagcccccgt gttcgaggag
      241 gcgatggtgc acacgaaggc cggctgcgcc gcgtcgcgca gcccgccgag cagggtgtcg
      301 tcaccttcga ccaggatcct tgccatattc cctccgatag atcgacctgt agatcattcg
      361 cgcccggcac cggacgccgc ccgctccgct acgacgccgg ccggggcgac gacgaaccgc
      421 ccaccggtgg gtcggagggc cagggcgagc cgtccacccg agtgtcggcg ggccgggtag
      481 attgctcacc ggcgggttga agagatccct gacccttgat cttgatggga atgccgctca
      541 cacacaggta gacctcatcg gccctgcgcg cgaacgcctg gttcacccgc ccctgaagat
      601 cgcggaagag gcggctcagc cgatccccgg ggacgatgcc cgtgcccacc tcgttcgtca
      661 cgaccacggc ttgcggccaa tgatcgctca gatagtcggc caagtcctgc atgacctggg
      721 cgagcgcgac ttccgggtcc gaggatccgg cttcctcctc caggagcagg gtggtcgtat
      781 agagcgtcaa gcagtcaaga aggagggtgt cccaatcccc ttcgacctct cgaagtcgct
      841 cgaccgcatc ccgcggagca accagggtgc cccactcggg cgaacgagcg gcttggtgac
      901 gctcgatgcg cagccgcatc tcctcgtcac ggggctcggc ggtagcgacg taaagcactc
      961 gaccgccgaa gtcagtggcg agtcgctccg ccaatgtcga cttgccgctg cgagcgccgc
     1021 cggtgacgag gatcatggag cgaagtgaag gtgcatgcca gccctttccg tcgaagagca
     1081 cgtccggcat gtctggcaac ggcaggtctc ctgactcccg gtttagacgc ccctttcccc
     1141 ttcccagccc gaggccggtg gatcttggag gtggctcacc ggttacagtg gcgagaccgc
     1201 gtcggcttct caccgacttc cctgttagcc gtctggcacc gtcgccgaca acaacctgtg
     1261 ccgcaggata tccgaaagcc cagggcgtca caagccggct ctctgcggac gacgccgcgt
     1321 cggcggcggt gtggagcgga gacctgggca gcgtcgcgag cccgccgcag atgagcgtga
     1381 acccaatacc cgagtattcg atcagcgcat ccgcgccccg acagaagctg tgggtcgcgg
     1441 tgacgacaac actcccaggg gccgaggagc tcgggcgttg tcgggcgacg gatcggcgaa
     1501 gggatcttgc ttgaaccaaa caaggtcggt cgcagctccg cggctcaggc gtccgtagcc
     1561 gccacccaga cccagcaggt cggcggccac ggcgcccacg gcagcgcgcg ccgcttccgg
     1621 agagaggccg gcggagcgta ggtgcgccac ctctgagagc acagccccgt gcgcaacgcc
     1681 gggggagccg gcgtcggtgc ccgccgcgat cctaacgccc gcggcgtggc cggccgccac
     1741 catcgcttcg tgtgcggcga tgatccgtgg caccgccgcc cacctacccg agaccgccag
     1801 ggcatgcagg ggagtgagcg tgggcaccca cacgacatcg tggtcgacca tggcgcgcac
     1861 cgactcctcg tcgatgagaa tgccgtgctc gacgctgtcg acgcccgctt cggcggccat
     1921 ggtcaccgcc tgggccccgt tcgcgtgcac cgcgaccgcc accccgagtt cgtgggcggt
     1981 ctccaccatt cttcggagtt cctcggagtg gaagtgggcg ggaggagcga ccccgcgctc
     2041 caggtcgacg cttcccgtga gtatcacctt gacgaccccg gctcctccgg ccacccg
//
LOCUS       DASRMJ010000062         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_405166, whole genome shotgun sequence.
ACCESSION   DASRMJ010000062 DASRMJ010000000
VERSION     DASRMJ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405166"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFD74_01820"
     CDS             complement(<1..600)
                     /locus_tag="VFD74_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK48325.1"
                     /translation="MDAKITARVPAAEVPAGAATAGEQGRPSRGRPRGYQLLRFVIQA
                     AVLLYVILAAIGHSVSWSWTANLHTICPFGGVENLYTYFTTGNYVAKLHDAVFVLLLG
                     LVAGLILTGKSFCGWICPLGTVQELLGKVSRFVRRGRPSVVLPRQLDRVLSYGKYAVL
                     AWVLVQTARTGRLAFEGYDPYYTLFNIWSDEIAWTGYLVV"
     gene            864..1391
                     /locus_tag="VFD74_01825"
     CDS             864..1391
                     /locus_tag="VFD74_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48326.1"
                     /translation="MRVGKKGLATGLMVAVLSLGVAVPAFAATTATAQTPGWAGGVCR
                     GTGAVADAVAGFLGLTPEAIAAERAAGQSLADIAEEQGKDTGGLVQTILSAKQGQIQQ
                     AVTDGRLTQERADSMLETMTERVTERVADPAMGPRGGDGGSRAGMGRGMGRAMGGGTG
                     SGMGFGGGLHQTDAL"
     gene            complement(1538..1756)
                     /locus_tag="VFD74_01830"
                     /pseudo
     CDS             complement(1538..1756)
                     /locus_tag="VFD74_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2184..2582
                     /locus_tag="VFD74_01835"
     CDS             2184..2582
                     /locus_tag="VFD74_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48327.1"
                     /translation="MTDRVGSRTVLLVPLARPPVQSGQPIRLLLEHVCPQDIGEEVVI
                     AIPATLVIEGNNEQVAALQALEHPWAMLLPGDGVAERTAQRVENGGLQQEVARALRLA
                     LKNLFDQVVQHETVAAGEGLNEAGGVRSPL"
     gene            2775..>3049
                     /locus_tag="VFD74_01840"
     CDS             2775..>3049
                     /locus_tag="VFD74_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48328.1"
                     /translation="MGVLPGGDDQVHLWRQMLQQKRESLVHWFGVDDMVVVEDEDDTV
                     RERADLVEQSRQHRFGRWRLGRVEHAQHPCPNISRNRPQSGREVGQEA"
BASE COUNT          573 a          971 c         1025 g          480 t
ORIGIN      
        1 cacgacgagg tatccggtcc aggcgatctc gtcgctccat atgttaaaga gggtgtagta
       61 aggatcatac ccctcgaaag cgagcctccc cgttctcgcg gtctgcacga gcacccaggc
      121 aagaaccgcg tatttgccgt agctcaatac gcggtcgagt tggcggggaa ggaccacact
      181 tgggcggccc cgtcgaacga agcggctcac cttgccgaga agctcctgga ccgtccccaa
      241 aggacagatc cacccgcaaa aagacttacc cgtgagaatg aggccggcca caagtccgag
      301 tagcagaacg aagacggcgt catggagttt cgccacgtag ttgccggtgg tgaagtacgt
      361 gtagaggttc tcgacaccgc cgaacgggca gatcgtatga agattcgccg tccaggacca
      421 gctgaccgag tggccgatcg cagcaaggat gacgtagagg agtacggccg cctggattac
      481 gaagcggagg agttggtagc ctcgtggcct ccctcgagac ggccggcctt gctcccccgc
      541 ggtcgctgcc cccgccggca cctccgccgc cggcaccctc gccgtgatct tcgcgtccat
      601 tcgacctcca gtttgagtca tttcgaggga agggcgcgaa cggcccgccg ctgtgtaccg
      661 ggaagaccgt acatttcgaa gattggcaaa ctgtgatgag gcggtaagga agcggtgagt
      721 gcgaacggcc ggacgacacc tactgcggac gactcctact gattcctggc ttccccctca
      781 cacttctcgc acacagcgcc cctaacctga cgttacaggg caaccgtcgg acgggagccc
      841 actagacgaa cgggaggcat cgtatgcgtg ttggaaagaa gggcctggcg accggcctga
      901 tggtggccgt gctcagcctc ggagtggcag tgccggcttt cgcggccacg acagcgactg
      961 cacaaacgcc tggctgggcc ggcggcgtgt gccgcggcac gggcgccgtg gccgacgccg
     1021 tggcggggtt cctgggtcta acgcccgagg cgatcgccgc cgagcgggct gccggccaga
     1081 gcctggccga catcgccgaa gaacagggta aggataccgg agggctcgtc cagaccatcc
     1141 tctcggcgaa gcaaggacag atccagcagg cggttacgga cggacggctc actcaggaac
     1201 gcgctgattc aatgcttgag accatgaccg aacgcgtcac cgagcgggtc gctgacccgg
     1261 ctatggggcc gcgtggcgga gatggcggtt cgcgcgccgg catgggtcgc ggaatgggtc
     1321 gcgccatggg cggcgggacc ggctcgggga tgggctttgg cggcggtctc caccagacag
     1381 acgccctcta accctacagg ttcactcagc cccgcaccga gggccggcgc tgttcccagc
     1441 gccggccctt cgtccttctc aagacattcc gcgagaggga gcgaggcccc atgcccgcgc
     1501 cgcgggcatg gggccgaccc agctctcctg caagccctta ggcttgcgtc gactcgatgc
     1561 gcatgcggta gaacgaacgc cacacgaagg tcacggatac caggaagaac accgcggtca
     1621 ggatgtagtt gagggcgtgg ccgccccgat cgtctgcgat gctcacggca agctcgacgg
     1681 ccagcaggcc gaacagcgtg gtgaacttga tgaccgggtt catggccacc gacgaggtgt
     1741 ccttgaaggg gtcgccgccg gccgcccgta atgcctccac aatgtctggc ggatgccggg
     1801 gttagcaggc acccgtgaca ttcggggcta cacactcgaa tcagggcata gtaggcacgg
     1861 gccacagcta tggtcgccag tagagaccga agctgttctt cgtccacgac gaccgcccgg
     1921 gaggtcgact gtccgtccga ctcgtcgggc gttttcagcc agcgctcggc gtccgtcagc
     1981 cgggtctcgg cagcctcgat ctcgcctcga cccaacaatg cgtaggcata ccacacgccg
     2041 agcacaggcc gggcgcggat ctgctcgtcc ggcagcgccc tcgcccagtt gaaccacgta
     2101 gcggtctggg agctctcctc caccagaggt cctgcccgct cgatcaggtc tgccgcccac
     2161 ccgaagtcct gggcgcgcaa agcgtgacgg atcgcgtcgg atcgcgaacc gttctcctcg
     2221 taccactcgc tcgccctccg gtgcagagcg ggcagccgat caggctgctc ctcgagcacg
     2281 tgtgcccgca ggacatcggc gaagaggtgg tgatagcgat accagcgacg ctcgtcatcg
     2341 aggggaacaa cgaacaggtt gccgcgctcc aagcgctcga gcatccctgg gccatgcttc
     2401 tgcccggtga cggcgtcgca gagcgcaccg cacaacgtgt cgagaatggc ggtctgcagc
     2461 aagaagttgc gcgcgcgctc cggctggcgc tgaagaacct cttcgaccaa gtagtccagc
     2521 acgaaacggt ggctgccggt gaaggtctga atgaagccgg cggcgtccga tcgcccctgt
     2581 agggagagcg ccgcgagttg caggccggcg atccagccct cggtgcgggt ctccagggca
     2641 gtgatgtctt ctgccgagag gtcgaggccc atcacccggt tgaggaactc ggcggcttcg
     2701 gagggggtga aacgcaggtc ggccgcgcgc agctcagtca gttggccccg agcgcgtagc
     2761 cgggccaggg gtagatgggg gtcctcccgg gtggcgatga ccaggtgcac ctgtggcggc
     2821 agatgctcca gcagaaacgc gagagcttgg tccactggtt cggagtcgac gacatggtag
     2881 tcgtcgagga cgaggacgac accgtgcggg agcgtgctga tctcgttgag caaagtcgtc
     2941 agcatcgatt cggtcggtgg cggctgggga gagtggagca tgcccaacac ccctgcccca
     3001 acatcagccg caaccgtccg cagagcggcc gcgaggtagg ccaggaagc
//
LOCUS       DASRMJ010000063         5340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_476045, whole genome shotgun sequence.
ACCESSION   DASRMJ010000063 DASRMJ010000000
VERSION     DASRMJ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476045"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1443
                     /gene="tig"
                     /locus_tag="VFD74_01845"
     CDS             61..1443
                     /gene="tig"
                     /locus_tag="VFD74_01845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZK48329.1"
                     /translation="MKTTVGEKVGNDVKLEIEVSTEEVQRGIEATIKQLSREVRIPGF
                     RKGKVPTGMIVQRFGMEAITQQMLDDQLSSWLVEALTEVEIEPVGRPEVDFEEAPEEG
                     KPFTFTSVVTVMPTAELGVYKGVEVPRVDAQVEDAEVDERVERLREEFAELESVGDRP
                     VAVGDFITVTAEGFRDGEAVPNTQLDDYMFEVGGGHLLPDLEQGVVGMKAGDECTLPV
                     AFPDDYHAEELKGATLDFTVAVKEIKEKSPPALNDEFAKDVSEFETLLELRLDIRAKL
                     QAAKDSAAQRRFRAAAIDAVAGAITVDLPPAAVDRQAREMVDDFARSLSMQGGDIRGY
                     LQATGSGVEQLVEQMMPEAESALRTSLALDAVAAAEGLEVTDEQLDERLQKLAAAGKV
                     EASELRSRLEQSGRITKIRQQILRERAAEFIAENAVAGDAVQPAAEQPEEGPEEVAAL
                     TGSDAQSAAE"
     gene            1484..2068
                     /gene="clpP"
                     /locus_tag="VFD74_01850"
     CDS             1484..2068
                     /gene="clpP"
                     /locus_tag="VFD74_01850"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZK48330.1"
                     /translation="MSPLIPMVVEQTSRGERAFDIYSRLLSERIVFLGTPVNDQVANL
                     IVAQLLHLESEDPDKDIALYVNSPGGSVYSGLAIYDTMQFIKPDVSTICIGVAMSMGA
                     LLLAGGAKGKRYVLPNSKVLIHQVSGGFQGPASDIEIHAREALSLRRRLDEILAKHSG
                     QELEKVEKDTERDYFMTAQEALDYGIVDKIIEHR"
     gene            2079..3347
                     /gene="clpX"
                     /locus_tag="VFD74_01855"
     CDS             2079..3347
                     /gene="clpX"
                     /locus_tag="VFD74_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZK48331.1"
                     /translation="MPKPSGGSEQLLCSFCGKSQRQVKKLIAGPGVYICDECIDLCNE
                     IIDEELAPPVTLDLDNLPKPQEIYSVLSEYVIGQEEAKRTLSVAVYNHYKRVQMSSAD
                     DDVELAKSNILMVGPTGCGKTLLAQTLARILNVPFAIADATALTEAGYVGEDVENILL
                     KLIQAADYDLKKAETGIIYIDEVDKIARKADNPSITRDVSGEGVQQALLKILEGTTAS
                     VPPQGGRKHPHQEFLSIDTTNILFICGGAFAGLEKIIERRIGKKGVGFGAHIEGKLDK
                     DVGELFAQVLPEDLLEFGLIPEFIGRLPVVSNVHQLRRDDLVAILTEPKNALVKQYRK
                     FFAYDGVDLIFPKDSLDAIADKAVIRDTGARGLRSIIEGALLNVMFELPSRRDVIKCV
                     ITKEVIERSIEPTLVTSAGREELELNEESA"
     gene            3364..>5340
                     /gene="lon"
                     /locus_tag="VFD74_01860"
     CDS             3364..>5340
                     /gene="lon"
                     /locus_tag="VFD74_01860"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZK48332.1"
                     /translation="MADVQRALPLLATRDLIVFPRMLTPIFEVRERSTRALEQAYATG
                     SALVLSAQRLGSTEDPGEADIFEVGTLCKVVQLHRLADGTVKAVIEGQRRVEIESFES
                     ESPMFLVNVRDLHTRPGREWSRTRALIDSVAREFAVYVSQSPQLPEEAETVLDDMTDP
                     ETVVDVVAPHLDIAPDRKQLLLEGEEAEERLLRLLEVLIRENEFLAIENEIADQVHEN
                     LERHQRKVFLRERLRVLREELGDEEDEGGDQGEYAKLMQEKQLPEAIAAALTKEIKRL
                     GELPPFSAEVAVAKTYLDHVLGLPWGKLSGAELPDVEKVARVLDETHYGLKDVKDRIV
                     EYLAVARLRGGTPPNTTLCLIGPPGVGKTSVAMAIAKGLERPLQRISLGGVRDEAEIR
                     GHRKTYVGAMPGRIVDALKRAGVDDPVILLDELDKVESDWRGDPAAALNEVLDPVQNQ
                     AFRDTYLELEYDLSRVLFVATANYEEDIPETLHDRLEMIRLPGYTDREKEEIARRHLA
                     PRLAEESGLGDVGDVFTSGARRVLIGSYTREAGVRELARLASKVYRKLARLRVEGRPV
                     PTRIGPRDVERLLGAPPFLRARVESTPMVGVSTGLAYTGAGGDILRIETVVAPGKGDF
                     HLTGQLGEVMQESVTAAWGYLKTALARDPELSELW"
BASE COUNT         1052 a         1603 c         1826 g          859 t
ORIGIN      
        1 ccacgataac aacccgggcc tgccgcgtga cgcggccggc acatggaagg aaggaccgag
       61 gtgaagacca ccgtaggcga gaaggtcggc aacgacgtca agttggagat cgaggtctcg
      121 accgaagagg tgcagcgtgg catcgaggcc accatcaagc agctctcgcg cgaagtgcgc
      181 atcccgggat tccgaaaggg caaggtgccc accgggatga tcgtgcagcg cttcggcatg
      241 gaggcgatca cccagcagat gctcgacgat cagctctcgt cgtggctcgt ggaggctctc
      301 accgaagtcg agatagagcc ggtcgggcgc cccgaggtcg atttcgagga ggcgccggag
      361 gaggggaagc cgttcacctt cacctctgtg gtcaccgtga tgccgacagc ggaactcgga
      421 gtctacaagg gtgtcgaggt gcctcgcgtc gacgcccagg tcgaagacgc ggaagtcgat
      481 gagcgcgtgg aacgcctgcg tgaggagttc gcggaactcg agtcggtggg cgaccgcccg
      541 gtggccgtgg gcgacttcat caccgtgacc gccgaaggct tccgcgatgg tgaggccgtg
      601 cccaacaccc aactcgacga ctacatgttc gaagtgggcg gcggacactt gttgcccgat
      661 ctcgagcaag gcgtcgtggg tatgaaagcg ggagacgagt gcacgttgcc ggtcgccttc
      721 cccgacgact atcacgctga ggagttgaag ggcgccacgc tggacttcac ggtcgccgtc
      781 aaggagatca aggagaagtc gccgcctgcg ctgaacgacg agttcgccaa ggacgtgagt
      841 gagttcgaga cgttgctcga actgcgcctg gacatccgag ccaagttgca ggcggcgaag
      901 gattcggccg cccagcgacg cttccgagcc gcagccatcg acgcggtggc gggcgccatc
      961 accgtggacc tacccccggc tgccgtcgat cggcaggcgc gggagatggt ggacgacttc
     1021 gcgcgctcgc tctcgatgca gggcggcgac atccgcggct atctgcaggc cacgggctca
     1081 ggcgtcgagc aactggtgga gcagatgatg cccgaagcgg aaagcgcgct gcgcacgagt
     1141 ctcgccttgg atgcggtggc ggcggccgag ggcctcgaag tcaccgatga gcaactcgac
     1201 gagcgtttgc agaaactggc cgccgcgggc aaggtcgagg cgtccgagtt gcggtcgcgc
     1261 ctggagcagt ctggtagaat cactaagata cggcagcaga tactgcggga gcgtgcagct
     1321 gagttcatcg cggagaacgc ggtggccggg gatgcggtgc aacctgcggc cgagcaaccc
     1381 gaagaaggtc ctgaagaggt tgccgccctg accggatcgg acgcgcagtc ggcggccgag
     1441 tgaccctccc ggcacgaccc cccgaaagcg aaaggatggc agtgtgagcc cactgatccc
     1501 catggtggtc gagcagacca gcagaggcga acgtgccttc gacatctact cgcgcctgtt
     1561 gagcgagcgc atcgtgttct tgggcactcc ggtgaacgat caggtggcga acctgatcgt
     1621 ggcgcaactc ctgcatctgg agtcggaaga cccagacaag gacatagcgc tctacgtcaa
     1681 tagccctggc ggcagcgtct actccggtct tgcgatctac gacacgatgc agttcatcaa
     1741 gccggacgtc tccaccatct gcatcggcgt ggcgatgtcg atgggagcgt tgctccttgc
     1801 cggcggtgcg aagggaaaac gctatgtgct gccgaactcc aaagtgctga tccatcaggt
     1861 ctccggcgga ttccagggcc ccgcgagcga tatcgagatc cacgcccggg aagcgctgag
     1921 cctgcggagg cgcctggacg agatcctcgc gaagcactcg ggccaagagc tcgagaaggt
     1981 ggagaaggac accgaaaggg actacttcat gaccgcgcag gaggctctcg actacggcat
     2041 cgtggacaag atcatcgagc acaggtaggg agtagccagt gcccaagccg tcgggtggtt
     2101 ctgaacagct cctctgcagc ttctgcggga agagtcagcg ccaggtcaag aaactcatcg
     2161 ccggacctgg cgtgtatatc tgcgacgagt gcatcgatct ctgcaacgag ataatcgacg
     2221 aggagttggc cccgccggtc acgctcgacc tggacaacct cccgaaaccg caggagatct
     2281 actctgtgct gtccgagtac gtcatcgggc aggaggaggc caagcgcacg ttgtcggtag
     2341 cggtgtacaa ccactacaag cgcgtgcaga tgagctccgc cgacgacgac gtggagctgg
     2401 ccaagtcgaa catcctgatg gtggggccca ccggttgcgg caagaccctg ctggcgcaga
     2461 cgctggctcg gatcctcaac gtgccgttcg ccatcgcgga tgccacagcg ctcaccgagg
     2521 ccggctacgt gggcgaggac gtcgagaaca tcctgctcaa actcatccag gccgccgact
     2581 atgacctcaa gaaggccgag acgggcatca tctacatcga cgaagtcgac aagatcgcgc
     2641 gcaaggccga caacccgtca atcaccaggg acgtgtcggg tgagggggtc cagcaggctc
     2701 tgttgaagat cctcgagggc accacggcga gcgtacctcc gcaagggggc cgcaagcacc
     2761 cgcaccagga gttcctcagc atcgacacca cgaacatcct gttcatctgc ggcggcgcgt
     2821 tcgccggact tgagaagatc atagagaggc gcatcgggaa gaagggcgta gggttcggtg
     2881 cgcatatcga gggcaagctc gacaaggatg taggcgagct gttcgcgcag gtgcttccgg
     2941 aagacctcct tgagttcggg ctgatcccgg agttcatcgg ccgcctgccg gtcgtctcga
     3001 atgtgcacca gctgcgcagg gacgatctcg tggccatcct gaccgaaccg aagaacgccc
     3061 tggtcaagca atatcgcaag ttcttcgcct acgacggggt ggacctgatc ttcccgaagg
     3121 acagcctgga tgcgatcgcc gacaaggcgg tgatccgcga cacgggcgcc cgaggactga
     3181 ggtccatcat cgagggcgcg ctcctgaacg tcatgttcga attgcccagc cgccgcgacg
     3241 tgatcaagtg cgtcatcacg aaagaggtca tcgagcggag catcgagccc accttggtca
     3301 cgagcgccgg tcgggaagaa cttgaactga acgaagaatc ggcctagctc tggagccatg
     3361 attttggcag acgtccagcg tgcgctgcct ctccttgcga cacgcgatct cattgtcttc
     3421 ccgcgcatgc tcacgcccat attcgaagtg cgcgagcggt ctacccgcgc gctggaacag
     3481 gcgtatgcga ccggctcggc tttggtgctc tccgcgcaga gactgggatc caccgaagac
     3541 cccggtgagg cggacatctt cgaagtgggc acgctgtgca aggtcgttca gctgcatcgg
     3601 ctggccgacg gcacggtcaa ggcggtcata gaggggcagc ggcgcgtcga gatcgagtcg
     3661 ttcgaatctg agtctccgat gttcttggtg aacgtgcgcg acctgcacac gcgtccgggc
     3721 agagagtggt cgaggacccg tgcgttgatc gactcggtcg cgcgggagtt cgccgtgtat
     3781 gtgagtcagt cgccccagct tcccgaagag gcagagacgg tcctggacga catgaccgac
     3841 ccagagaccg tcgtggatgt ggtggcgccc cacctcgaca tagcgcccga ccgcaagcag
     3901 ttgctgctcg agggtgaaga ggccgaggag cgtctcctgc gcctcctcga ggtgctgatc
     3961 cgcgagaacg agttccttgc tatcgagaac gagatcgccg accaggtgca cgagaacctg
     4021 gaacgccacc agcgcaaggt gttccttcgc gagcgactgc gggtgcttcg tgaggagttg
     4081 ggtgacgagg aagacgaggg cggcgaccag ggcgaatacg cgaagctgat gcaggagaag
     4141 cagctgcccg aggcgatcgc cgcggctctg accaaggaga tcaagcgcct gggagagctg
     4201 ccgcccttct cggccgaggt cgcggtggcc aagacgtatc tggaccacgt gctgggcctg
     4261 ccctggggca agctttcagg tgcggagttg cccgacgttg agaaggtggc tcgagtgctc
     4321 gacgagaccc actacggtct caaagatgtt aaggatcgca tcgtcgagta cctggccgtg
     4381 gcgcgcctgc ggggcggcac gccgccgaat accaccttgt gcctgatcgg ccctcccggt
     4441 gtgggcaaga cctccgtcgc catggccatc gccaagggtc tcgagcggcc gctccagagg
     4501 atcagcctcg gtggcgtgcg cgacgaggcc gagatcagag gccaccgaaa gacctacgtg
     4561 ggggcgatgc cgggtcgcat cgtcgacgcc ctgaagcggg cgggtgtgga cgatcccgtc
     4621 atcctgctcg acgagttgga caaggtggag agcgattggc gcggcgaccc ggccgccgcg
     4681 ctcaacgagg tgctcgatcc ggtgcagaac caggccttcc gagacaccta tctggagctg
     4741 gagtacgacc tgtcgcgggt cttgttcgtg gccacggcca actatgagga agacatcccg
     4801 gagactcttc acgatcgctt ggagatgatc cgcctccccg gatataccga tcgggagaag
     4861 gaagagatcg cgcgccggca cctcgcccct cggcttgctg aggagagcgg gctgggagac
     4921 gtgggcgacg tcttcacctc aggagctagg cgagtgctca tagggtccta cacccgcgag
     4981 gccggagtga gggagctggc gcgcctggcg agcaaggtgt atcggaaact cgcgcgcctg
     5041 cgggtcgaag ggcgcccggt gcccactcgc atcggacccc gcgacgtgga gcgcctcctc
     5101 ggggctcctc cgttcctgcg cgctcgggtg gagagcacac cgatggtggg cgtgagcacc
     5161 ggattggcct acaccggcgc cggcggggat atcctgcgca tagagacggt cgtggcaccg
     5221 ggcaagggcg atttccacct caccgggcaa cttggggagg tcatgcagga gagcgtgacg
     5281 gccgcctggg gctacctgaa gacggccctc gcccgagacc cggagctgtc ggagctgtgg
//
LOCUS       DASRMJ010000064         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_366328, whole genome shotgun sequence.
ACCESSION   DASRMJ010000064 DASRMJ010000000
VERSION     DASRMJ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366328"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFD74_01865"
     CDS             <1..232
                     /locus_tag="VFD74_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK48333.1"
                     /translation="SFTVGDIMSRDVAKCTAGDSVKSVMETMSRRHIRHVPVVDGTDL
                     VGIISQRDVMKSILDETQLEMSVLRDIVIAKG"
     gene            277..2205
                     /gene="dnaX"
                     /locus_tag="VFD74_01870"
     CDS             277..2205
                     /gene="dnaX"
                     /locus_tag="VFD74_01870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZK48334.1"
                     /translation="MSISLYRKYRPTRFNDVVGQTHITRTLVNAIKQERVSHAYLFAG
                     PRGTGKTSTAKILAMALNCEAGHGKATTEPDGTCPCCEGIRRGSSLDVLEIDAASNRG
                     IDEIRDIRDRVSFAPVEGRMKVYIVDEVHMLTTEAFNALLKMLEEPPAHVVFVLATTE
                     PHKVLSTILSRCQRFDFRRPSAQEITGVLASVADREGIDVSESALAVIARAASGSFRD
                     ALGTLDQLSAYCEGKVSLQDALALLGVADQDLLFELVDVVDERDTTAALLFVERLAQS
                     GTDLNQFLKDLLSHLRDLYVVRHTTEPPDSIVSSPEHLDYLRSQANRVSTSELVAFID
                     LIGEAQRAIRTGADPRLELELSLIKLTRPATDSSLRGILSRLDSLESGGSAEPDTTRP
                     FRGGSAARSLEPSAGAGGGRHVTAADSASHSAASDKRRRLTSVPPSIGPKDEAAGGVG
                     GAGGVGGPEATRGPGSTRGQDADDARGDTGDSGRTDVADGLGDDAPDGRSEDAPRRGD
                     DPGTTPHIEPNIDNLRRAWPVILARVKNQRPSLFAVLSESRPESLDQDALTLKFPQGA
                     DFAAAQASKQDNLAFLTDILADVTGRRFKVVIRLAGTAAEVEEPNEGRARILTKEELL
                     EILKQEFDARPVDDDRAP"
     gene            2226..2543
                     /locus_tag="VFD74_01875"
     CDS             2226..2543
                     /locus_tag="VFD74_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZK48335.1"
                     /translation="MNPNYQKMMKQVQKMQTDMAKAQEDLALEHVEASAGGGMVVVRV
                     SGSLEVVGVKIDPAALDPDDVEMLEDMVIAATNEAVRQAQDLQAKRLGGLTAGLNIPG
                     LSM"
     gene            2645..3256
                     /gene="recR"
                     /locus_tag="VFD74_01880"
     CDS             2645..3256
                     /gene="recR"
                     /locus_tag="VFD74_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZK48336.1"
                     /translation="MYSPPIQRLIDELAKLPGIGPKTAQRLTFHLLRLPPEDALPLAH
                     ALIEAKEAIHFCTRCFNLTAEELCEICRDPRRDPTLVVVVEEPSDIVALERAHQVRGL
                     YHVLGGALSPIDGIGPDRLRLAQLFDRVEAEGLTEIVVATNPTMSGEATALYIAEELR
                     PLSEAGRVRVTRPAAGLPMGSDLEYADEVTLGRALAARSDILR"
BASE COUNT          635 a         1209 c         1145 g          541 t
ORIGIN      
        1 ctcgttcacg gtgggagaca tcatgtcgcg cgacgtggcg aagtgtacgg ccggcgattc
       61 ggtgaagtcg gtcatggaga ccatgtctcg gcgtcacatc aggcacgtgc ccgtcgtcga
      121 cgggaccgat ctcgtcggca tcatcagtca gcgcgacgtc atgaagagca tcctcgacga
      181 gacgcagttg gagatgagcg tcctgcgcga catcgtcatc gccaagggct agtcaacgcc
      241 ttcgctcccc gccgaccagc aaggagccgc ccagccatgt cgataagcct ctacaggaag
      301 taccggccga ctcgtttcaa cgacgtggtc ggtcagacgc acatcacccg cacgctggtc
      361 aacgccatca agcaggagcg ggtgagtcac gcatacctgt tcgcgggccc ccgcggcacc
      421 ggcaagacgt cgaccgccaa gatcctggcc atggcgctca actgcgaggc cgggcacggg
      481 aaggccacca ccgagcccga tggcacctgt ccctgctgcg aaggtatccg ccggggcagc
      541 tctctcgacg tgctcgagat cgacgcggcc tccaaccgcg gcatcgacga gatccgcgac
      601 atccgcgatc gggtgagctt cgcgcccgtc gaagggcgca tgaaggtgta catcgtggac
      661 gaggtgcaca tgctcaccac ggaggccttc aacgcactgc tcaagatgct ggaggagcca
      721 cccgcccatg tcgtgttcgt gctggccacc acggaaccgc acaaggtact ctccaccatc
      781 ctctcgcgct gtcagcgctt cgactttcgc cgcccctccg cccaggagat aaccggcgtg
      841 ttggcttcgg tggccgatcg ggagggaatc gacgtgtcgg agagcgcgct cgcggtcatc
      901 gcccgcgccg cgtcgggcag tttccgcgac gccctgggca ctctcgatca gctctcggcc
      961 tactgtgagg gcaaggtctc gcttcaggat gccctggccc tgctgggcgt cgccgaccag
     1021 gacctgctct tcgagttggt cgacgtggtg gacgaacgtg atacgaccgc cgcgctgctg
     1081 ttcgtggagc gcctcgccca atcaggcacc gacctcaacc agttcctgaa ggacctgctc
     1141 agccatctcc gcgacctgta cgtcgtgcgc cacaccaccg aaccgcccga cagcatcgtg
     1201 agcagcccgg agcacctgga ctacctccgg agccaggcga accgggtctc gacgtcggaa
     1261 ctcgtggcgt tcatcgacct catcggcgaa gcccagcggg cgatccgcac gggagccgac
     1321 ccccgcctcg agttggagtt gtcgctcatc aagctcacga ggcccgccac ggactcctcc
     1381 ctgcgcggca tcttgagtcg gttggactcc ctcgagtcgg gcggatccgc agagcctgac
     1441 acgacgagac ccttcagggg tggcagcgca gcgagatccc tcgaaccatc agcgggcgcg
     1501 ggcggcggca gacacgtcac cgccgctgac tcggcaagcc actccgccgc ctccgacaag
     1561 cggaggcgac tcacgtcggt gccgccgtcc atcggaccga aggatgaggc cgcgggcggc
     1621 gtgggcggtg ccggcggcgt gggcggtcca gaagcaacgc gcggccccgg tagcacacgc
     1681 ggccaagatg cagacgacgc gcgcggtgac accggcgact cggggcgcac cgatgtcgcg
     1741 gacggcctcg gagacgacgc ccctgacgga cgttccgagg acgcgccgcg ccgcggggac
     1801 gatccgggga cgacgccgca catcgagccc aacatcgaca acctgcggcg tgcgtggccg
     1861 gtgatcctcg cgcgagtgaa gaaccagcgt ccgagcctct tcgcagtgct cagcgaaagc
     1921 cgtccggagt ctctcgacca agacgccctg accctcaagt tcccccaggg agccgacttc
     1981 gcggccgcgc aggcctccaa gcaggacaac ctggccttcc tcaccgacat cctggccgac
     2041 gtcacgggta ggcgcttcaa ggtggtcatc aggctggctg gaacagcggc cgaggtggag
     2101 gaacccaacg agggccgtgc tagaatcctg accaaggagg agctgctcga gatcctgaag
     2161 caggagttcg acgcccggcc cgtcgacgac gatcgcgctc cctgacactc caggcgagga
     2221 gacccatgaa tccgaactac cagaagatga tgaagcaggt gcagaagatg cagaccgaca
     2281 tggccaaggc ccaggaggac ctcgcgctcg agcatgtaga agcgtcggcg ggcggcggga
     2341 tggtcgtggt ccgcgtgagc ggttcccttg aggtcgtagg cgtgaagata gatccggccg
     2401 ccctcgaccc cgacgacgtg gagatgctcg aggacatggt catcgccgcg accaacgaag
     2461 cggtccggca ggcgcaagac ctccaggcca agcgcttggg cggtctgacg gccggtctca
     2521 acatcccggg actgagtatg tagctcggcg gagcagccgg gcgggcgacc atcccgacgt
     2581 cgctcgacca gacctgtgcc cttccatccg gatccgatct ctcccgacca gaccgaggcg
     2641 caccctgtac agtcctccta tccagaggtt gatcgacgag ctggcgaagc tcccgggcat
     2701 cggcccaaag accgcccagc ggctcacctt ccacctcctc cgactgcccc ccgaggacgc
     2761 gctgccgttg gcgcacgcct tgatcgaggc aaaggaggcg atccacttct gcacgcggtg
     2821 cttcaacctg acagccgagg agttgtgcga gatctgccgc gatcccaggc gcgatcccac
     2881 cttggtcgtg gtggtggagg agccctcgga catcgtggcc ctggagcggg ctcaccaagt
     2941 gcgcgggctt tatcacgtcc tgggaggcgc cctctcaccc atcgacggca tcggccccga
     3001 ccgcctgcgc cttgcccagc tcttcgaccg cgtggaagcg gaaggcctca cggagatagt
     3061 ggtagccacg aaccccacga tgagcgggga ggccaccgcc ctctacatcg ccgaagagtt
     3121 gcgaccgctc tccgaggccg ggcgggtgcg cgtgacccga ccggcggctg ggctgcccat
     3181 gggcagcgac ctcgagtacg ccgacgaggt cacgctgggg cgggcactgg ccgcccgttc
     3241 cgacatcctg cgctgaagcc cgtcctcgcg tccgccacgc ggcatggttt cggcctcgcg
     3301 ggtcgaaggt atctaccgcg cgaccatcgc gcaaccgcat cgtcgtcaga acgcgcaatc
     3361 gtgatcggtg taatttcgtc gtcgccggga aaccatcgcc aagggggagt cttcatgggt
     3421 gtgggccgtt cgtttactta catgcttcag caaccgggag gactcggcaa gctcgtcata
     3481 ggcggattgc tgatgttcgt gccgatcatc ggctgggcca tcgtggccgg
//
LOCUS       DASRMJ010000065         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_441975, whole genome shotgun sequence.
ACCESSION   DASRMJ010000065 DASRMJ010000000
VERSION     DASRMJ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441975"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VFD74_01885"
     CDS             complement(<1..928)
                     /locus_tag="VFD74_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HZK48337.1"
                     /translation="MLELVKIVAVFALVVGLLRLRWNLGLVMLVAAAVLGVLMGMGPR
                     ALAETALRASIEPTTVSLVVALALIMVLEYILRTTGTLASLVSSLQGLLGDNRVVMGL
                     MPAIIGLLPSAGGARFSAPLVEESAKGCVIDPERKSYINYLFRHVWEYISPMYPGFIL
                     TATLAGVSMGRLFVWQAAFPATVLVMGIGFGFRGIETQPRHSGRTRSHDLRVAAAGFS
                     PILAAIVLVVGLGVNVAASMGIVVAVMLLAHRWGPARIYRAVRDSVSIKTLLLVLGVM
                     VFKGMMEDSGAVGHLPQALETAGVPVGIVLVAL"
     gene            complement(945..1964)
                     /locus_tag="VFD74_01890"
     CDS             complement(945..1964)
                     /locus_tag="VFD74_01890"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HZK48338.1"
                     /translation="MEARIIGLKGSGKSTLMTALAEGRSEGTITTVRMGDDRIRRLSE
                     MFKPRKTTFAEFRVREAAWPEASARKAQMERYLDQLSGGQVFLHVLRAFESPMLAEAA
                     DIRRDLQTLDSEFVFADLMIVERAMERSRKQPIPDLGKTAFGRCLEALESERPLRDVE
                     FDQSEADFLKTYQFLTLVPQIILVNLESDAAAGDAIHALLADIARGRQIVAFPFPEAA
                     EVAELTPAEQLEFAEAMGLPGPAAEIVSHAAFSQMGLISFFTVGEDEVRAWPIRRGER
                     ARQAAGAVHSDIERGFIRAEVVSYDTLLEAGSIKVCRDHGSLRIEGKDYVVADGDVMN
                     YRFNV"
     gene            complement(2349..2930)
                     /locus_tag="VFD74_01895"
     CDS             complement(2349..2930)
                     /locus_tag="VFD74_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZK48339.1"
                     /translation="MTALLTSFFLVGIAEMADKTQLLTLCLTCKYPARKVLLGVALAI
                     GLLNLAAVLVGGLAGQLLPVVPIKIAAGVLFLAFGAWTLLAPDKPQIEDAGDGTPGDS
                     RRRSAVLAVAVAFLIAEIGDKTQLATLSLAARYEAFVMVWLGASLGMLAANGLAIVGG
                     SLLSDRLPQTLLKRVSGVLFIAFGIWTLIDVLA"
     gene            2985..3401
                     /locus_tag="VFD74_01900"
     CDS             2985..3401
                     /locus_tag="VFD74_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HZK48340.1"
                     /translation="MSDSDYGIVRHVAVGYEEAVSLAREALKEQGFGVLTEIDVKKTM
                     KEKLDVDFKPYIILGACNPHLAHRALTAEPDIGLMLPCNIVVYEEGPGAATVEAISPM
                     AALGVVDNDDLKDVADQAQAKLTAAMDAVAERAGTI"
     gene            3497..4033
                     /locus_tag="VFD74_01905"
     CDS             3497..4033
                     /locus_tag="VFD74_01905"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZK48341.1"
                     /translation="MFQRQPWMQRAGEFPESDLQYHELLVRAVFSGGLGPRVVEARWE
                     GISEAFCGFRPEVVAALGEADVSRLLGDPKVIRNRRKIEAVIHNAAEFGVVVTQSGSF
                     HQHLADLEAGTDLVDAAAELAKRFKHLGPTSAALFLFSAGWREQQLDADEPADAHRQA
                     DAAPPARPRPTAVTATRR"
BASE COUNT          741 a         1270 c         1393 g          651 t
ORIGIN      
        1 gcagcgccac caagacgatg ccgacgggca cgccggcggt ctccagggcc tgaggcaggt
       61 gccccaccgc tcctgaatcc tccatcatgc ccttgaacac catgaccccc agcacgagca
      121 gcaaggtctt gatcgacacg ctgtcgcgga cggcccgata gatcctcgcc ggtccccacc
      181 tgtgcgccag cagcatgacg gcgaccacga tacccatgga cgcggcgacg ttgacgccga
      241 gcccgaccac caggacgatc gcggccagga tggggctgaa accggccgcc gccacgcgca
      301 gatcgtgact cctagtgcgt ccgctgtgtc ggggttgggt ctcgatcccg cggaagccga
      361 agccgattcc catcacgagc accgtcgccg ggaatgcggc ctgccacacg aacagtcggc
      421 ccatggagac gcccgcgagc gtggcggtca ggatgaagcc ggggtacatg ggcgagatgt
      481 actcccacac gtggcggaac aggtagttga tgtagctctt gcgctcgggg tcgatgacac
      541 accccttggc cgactcctcc accagcgggg cggagaagcg ggcgccgccg gcggagggca
      601 gcagacctat gatcgcgggc atcagcccca tgaccacgcg gttgtcgccc agcaggccct
      661 gcaacgaact caccagggac gcgagcgtac cggtggtgcg taggatgtac tcgagcacca
      721 tgatgagcgc caaggctacg acgagtgaga cggtggtcgg ctcgatggag gcgcggagcg
      781 cggtctcggc cagagcacgc ggacccatgc ccatgagcac cccaaggact gctgccgcga
      841 ccagcatgac caacccgaga ttccagcgca ggcgcaacag gcccaccacg agggcgaaca
      901 cggcgacgat cttgacgagt tccagcaagc gggctgcttc cgagctacac gttgaagcgg
      961 tagttcatga cgtctccgtc ggcgacgacg tagtccttgc cttcgatcct cagcgacccg
     1021 tggtcgcggc agaccttgat ggagccggcc tcaagcagag tgtcgtagct cacgacttcg
     1081 gctcggatga atccgcgctc gatgtctgag tgcaccgcgc ccgcggcctg tcgcgctcgc
     1141 tctcctcggc ggatcggcca ggcgcgcacc tcgtcctcac ccacggtgaa gaaactgatc
     1201 aaccccatct gcgagaacgc cgcgtgcgag acgatctcag ccgccggtcc gggaaggccc
     1261 atcgcctcgg cgaactccaa ctgctcggcc ggcgtgagtt cggcgacctc cgccgcttcc
     1321 gggaagggga aggccacgat ctgccgcccg cgcgctatgt cggcgagcaa cgcgtggatc
     1381 gcgtctccgg ctgcggcgtc cgactccagg ttgaccaaga tgatctgggg cacgagagtc
     1441 aggaattggt aggtcttgag gaagtcggcc tcggattggt cgaactccac gtcgcggagc
     1501 ggacgttcgc tctcgagcgc ctccaggcag cgaccgaacg cggtcttgcc gaggtccggg
     1561 atcggttgct tccgcgaccg ttccatggcg cgttccacga tcatgaggtc ggcgaacacg
     1621 aactcggagt cgagcgtctg cagatcacgc ctgatgtcgg cggcttcggc cagcatcgga
     1681 ctctcgaacg cccggagaac gtgcaggaac acctgtcccc cggagagttg gtcgaggtag
     1741 cgctccatct gtgccttgcg tgccgaggct tcgggccaag cagcctcgcg caccctgaac
     1801 tccgcgaagg tggtcttgcg gggcttgaac atctcggaga gcctgcgtat gcggtcgtcg
     1861 cccatcctga cggtcgtgat cgtgccctcg gagcgtcctt ccgccagcgc ggtcatgaga
     1921 gtggattttc cgctgccttt gaggcctatg atccgggctt ccacgtgggg tcgcctccct
     1981 tccgtcttgg ccggtagtat cccagaagtg gcgggttccc gtccgcgggc cggcgtttgg
     2041 gcgcgttcgg cagcggatga gaccgctccg cacggggtat gacgcggtgg atgccggtga
     2101 aagggggtcg tgttggagtt ctttctcgat tcggatctgg gccgcagatt ggatgaggct
     2161 ctcaccacgg agggggccgc gtacgtggag gcgtgggacc gcgcgctgcc tggatcaggc
     2221 gccgagtgca tgtgggtggg ctttcgtggt ggcccgcggc ttccgggtag cgtacaccca
     2281 ggtcatgatg gtgcggaact gcggacttgc gggcgggcgc ggaggctgct agaatacccc
     2341 caagggtatc aagccagcac gtcgatgagg gtccagatgc cgaacgcgat gaagaggacg
     2401 ccggagactc tcttgaggag ggtctggggc aatctgtcac tgaggagcga accgccgacg
     2461 atcgcgagtc cgttcgcggc gagcatcccc aggctcgctc ccaaccacac catgacgaag
     2521 gcctcgtatc gtgctgctag ggacaaggtc gccagctggg tcttgtcccc gatctcggcg
     2581 atcaagaagg cgacggcgac ggccagcacg gcgctgcgtc gccgactgtc acccggcgtt
     2641 ccgtcaccgg catcctcgat ctggggcttg tcaggcgcca ggagcgtcca cgcgccgaag
     2701 gccaagaaga gaacaccggc ggcgatcttg atggggacca cgggcaggag ttggcccgcc
     2761 aacccaccaa ccagcacggc ggccaggttg agcagtccga tggccaaggc gacgcccagc
     2821 aacaccttgc gtgccgggta cttgcaggtg aggcacaggg tgagcagttg ggtcttgtcg
     2881 gccatctcgg cgatgccgac caggaagaac gaagtgagca gagcggtcat ccggggcagg
     2941 atagcatgca cagtgagtga gagagtgaac gagaggagcg aatgatgagt gattcggact
     3001 acggcatagt gaggcacgtt gcagtgggct acgaggaggc ggtgtcgctt gcccgtgagg
     3061 cactgaagga gcagggattc ggcgtgctca cagagatcga cgtcaagaag acaatgaagg
     3121 agaagctcga cgtcgacttc aagccctaca tcatattggg cgcctgcaac ccgcatctgg
     3181 cccacagggc gctgacggcg gaacccgaca tcggtctgat gctgccctgc aacatcgttg
     3241 tgtacgaaga gggccccgga gccgccacgg tggaagccat ctcgccgatg gccgcgctgg
     3301 gcgtcgtcga caacgatgac ctgaaggacg tggcggacca agcccaggcc aagctcacgg
     3361 ccgccatgga tgccgtggcc gagcgggcgg ggacgatcta gccagtctcc aacctccttc
     3421 ctacagactt cccgcgtccg tctgcgtagg ctgcaaacgt ctcgccatga ggcgggcgcg
     3481 gaagagggga ggagcgatgt tccagaggca accctggatg cagagagccg gggagttccc
     3541 cgagagcgat ctgcagtatc acgagctgtt ggtgcgcgcg gtgttctcgg gcggtttggg
     3601 tcctcgggtg gtggaagccc gctgggaagg gatcagcgag gcgttctgcg ggttccgtcc
     3661 tgaggtggtg gcggctctgg gcgaggccga cgtgtcgaga ctgctcggcg atcccaaggt
     3721 gatcaggaac cggcgcaaga tcgaagcggt cattcacaac gccgccgagt tcggcgtcgt
     3781 cgtcacgcag agcggaagtt tccaccagca cctggccgac ctcgaggctg ggaccgactt
     3841 ggtcgacgca gccgccgaac tcgccaagag gttcaagcac ctgggtccca cttcggccgc
     3901 cctgttcttg ttcagcgccg ggtggcggga acagcagttg gacgcggacg agccggcgga
     3961 cgcgcatcgg caggcggacg cggccccgcc ggctcgtccg cgtccaactg ctgttaccgc
     4021 cacccggcgc tgaacaagaa cagggcggcc gaagt
//
LOCUS       DASRMJ010000066         4061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_258019, whole genome shotgun sequence.
ACCESSION   DASRMJ010000066 DASRMJ010000000
VERSION     DASRMJ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258019"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFD74_01910"
     CDS             <1..655
                     /locus_tag="VFD74_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZK48342.1"
                     /translation="FRRVVNLVRERCDIILNLTSSGGLIRDEDERIRPIAELAPELAS
                     FDAGSINFGRRVFLNAPDFLDRLAAVMLEKGVKPEIEVFDTGMIANALALVSNGLLRE
                     PLHFQFVVGVAGGMPATPRGLLHLVESIPPGSTWSAIGVGRAHLPIASMALHMGGHIR
                     VGMEDNVYYRKGVLARSNAEFVSRIVDLAEVFERPVATPTEARAILGLGATRPSVKD"
     gene            786..1751
                     /locus_tag="VFD74_01915"
     CDS             786..1751
                     /locus_tag="VFD74_01915"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZK48343.1"
                     /translation="MRIAIIGAGALGGTFGFLFADAGHDVTLVDVDRAKIDAIRRDGI
                     TLIMPDGTRRHRPMSITSDPESVGVVDLVQVSVKGYHTTSAAESAGPLIGPDTYVLSL
                     QNGLTNLERIAEVVGAEKVLGGVTAHSAMPLDHTLIRYNGGMGGVYFGRLDGGCDPGL
                     QRFVGLFASAGFESRLIEGDIREPIWRKLLANVSVNAVAALSGLTGREIQEIEPACEL
                     VRALARETAAVARAQGFTFPELDQPDDYVIKTLDWVGDNKVSMLQDTEAGRRTEIDTL
                     NLAVVECGERFGVDTPLNWAVGSLVKMREEKMLRDSRLLKVAGTV"
     gene            1754..1957
                     /locus_tag="VFD74_01920"
     CDS             1754..1957
                     /locus_tag="VFD74_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48344.1"
                     /translation="MNIFVALMLSLVGVGLLGYGRKQGRGLHIGAGLVLLVVPYFLRS
                     TVLEVVLGAVVLGALGLVSRLGY"
     gene            2098..3048
                     /locus_tag="VFD74_01925"
     CDS             2098..3048
                     /locus_tag="VFD74_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZK48345.1"
                     /translation="MQVDIIGGFLGSGKTTAILHLLKNGEFDPSRTVLLVNEFGEVGV
                     DGALLGGADGAGSVREIPSGCICCSLKLDFMSQLEDIARTLAPDRVVVEPSGVAATRD
                     ILQALTHERVAHLVGGIRTVLVLDPDDHDWFVEMSPTFVDSQIGLAQLILLNKTDLAD
                     PEDVARVTADLERRNPDAVVLPTSHGAFSWRAVEHILPPLPSPEGESARLEGFESFSA
                     RLPEALDAASLRGLFSELTDGRFGDVRRAKGVFLTGDGAGSVRFDLAARRVFETEWPR
                     TVPGRINVIGRDLDRQGIERSLAEAAVMAEARSGAERDPR"
     gene            3045..3416
                     /locus_tag="VFD74_01930"
     CDS             3045..3416
                     /locus_tag="VFD74_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48346.1"
                     /translation="MTAGDAARGAAPSGGDSTREREAGTVRREGWSRRRRILSFLGIV
                     GVATIVAGCAGQPPPASGSPGFFYGVLHGFLILVDFIVGLFSDVRIYAYPNSGGWHDF
                     GFLLGASMFLGGGGGAGARRR"
     gene            3423..3953
                     /locus_tag="VFD74_01935"
     CDS             3423..3953
                     /locus_tag="VFD74_01935"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZK48347.1"
                     /translation="MKETISIGDFTVRDVRVEDAPSIAAYADNPRVAANLRDVFPHPY
                     RLEDAQGFLAHVMQQLPPTVFAIATEAEAVGVIGLTLGEDVHRFTAELGYWLGEPFWG
                     RGVMTSAVRVVVAHGFRELGLLRIHADPYAGNVGSARVLEKAGFELEGRMRANVFKNG
                     RVLDQLLYSTIREGVA"
BASE COUNT          618 a         1292 c         1412 g          739 t
ORIGIN      
        1 gttcagacgc gtcgtcaacc ttgtgcgcga gcgttgcgac atcatcttga atctcacctc
       61 gtcgggcggg ctcatccgcg acgaggacga acgcatccgc ccgatcgccg agttggcccc
      121 cgagcttgca tccttcgacg cgggctccat caatttcggg cgaagggtgt tcttgaacgc
      181 tcccgatttc ttagaccggc tcgctgccgt catgctcgag aagggggtga aacccgagat
      241 cgaggtgttc gacaccggta tgatcgcgaa cgcactggct ctggtcagca acgggcttct
      301 ccgggaacca ctgcattttc agttcgtggt gggggtggcg ggaggcatgc cggccacacc
      361 ccggggcctg ctgcacctgg tcgagtcgat cccgcccggg tcgacgtggt cggcgatcgg
      421 cgtgggccgg gcccacctgc ctatcgcttc catggccctg cacatggggg gtcatatccg
      481 ggtcggcatg gaggacaacg tctactatcg caagggcgtt cttgctcgtt ccaacgctga
      541 gttcgtctcg cgcatcgtcg atcttgcgga ggtcttcgag cgtcctgtgg cgacgccgac
      601 cgaagcgcgc gcgattctgg gactgggtgc gacccggccg tcggtgaagg actgatcctt
      661 ggggccattc cgctgggaca ccctgcgcgg tagactggag tccgcgaccg accagacgac
      721 tgggaggcgc gggacttgcg ggtagccgac cagacgactg ggaagcgccc agggggaata
      781 cgaagatgag aatcgccatc atcggcgccg gcgccttggg gggcacgttc ggcttcctct
      841 tcgccgacgc cggccacgac gtcaccttgg tcgacgtcga ccgcgccaag atcgacgcga
      901 tccggcgtga cggcatcacg ttgatcatgc ccgacggcac acgtcggcat cgcccgatgt
      961 ccatcacgtc cgatcccgag tcggtggggg tggtcgacct cgtgcaggtg tccgtcaagg
     1021 gctaccacac gacctcggcc gccgaatcgg cgggcccgtt gatcggcccc gacacgtacg
     1081 tgctgtctct ccagaacggg ctcaccaact tggagcgcat cgccgaggtg gtgggcgcag
     1141 agaaggtgct cggcggggtc acggcccaca gcgccatgcc actggaccat acgctgatcc
     1201 gatacaacgg cggcatgggc ggtgtgtact tcgggaggct cgacggtggg tgcgaccccg
     1261 gcctccagcg gttcgtcgga ctcttcgcgt ccgccggctt cgagagtcgg ctgatcgaag
     1321 gcgacatccg ggagcccatc tggcgcaagt tgcttgccaa cgtctcggtg aatgcggtgg
     1381 cggctctctc tggactcacg ggccgagaga tccaggagat cgagccggcg tgcgagttgg
     1441 tgcgcgccct cgcccgcgag acggcggcgg tggctcgagc ccaagggttc acgttcccgg
     1501 aactcgacca gccggacgac tacgtcatca agaccctcga ctgggtgggc gacaacaagg
     1561 tctcgatgct gcaggatacc gaggcggggc ggcgcacgga gatcgacacg ctcaacttgg
     1621 cggtcgtcga gtgcggggag cgcttcggcg tcgatactcc gctgaactgg gccgtgggat
     1681 cgctcgtgaa gatgcgcgag gagaagatgc tgcgcgattc gcgactcttg aaggtggccg
     1741 gcacagtctg acgatgaaca tcttcgtcgc cctcatgctc agtctggtcg gcgtcggtct
     1801 tcttggctac ggcaggaagc aggggcgtgg gcttcacatc ggcgcgggtc tggtgcttct
     1861 ggtcgtcccc tacttcctcc gttccaccgt gctcgaggtg gtcctgggcg ccgttgtcct
     1921 gggcgccctc ggtctggtct ctcgccttgg atactgacgg agccctggcg cggtgactgg
     1981 aggcggcctt cagccgtgaa cgtcaccggg cgcgactccg ggactcatgc cggggtgtga
     2041 ctctccgact cgccgggcac actgggagca ggcaatcgac gtggggagac ggaagcgatg
     2101 caggtggaca tcatcggagg gttcctgggt tccggcaaga cgaccgcaat cctgcatctt
     2161 ctcaagaatg gtgagttcga tcccagccgg accgttctgc tggtgaacga gtttggcgag
     2221 gtgggcgtcg acggagccct gctcggcggc gccgacggcg cgggttccgt gcgcgagatc
     2281 ccgagcggct gtatctgctg cagcctcaag ctcgacttca tgagccaact cgaggatatc
     2341 gcccgcactc tggcccccga ccgtgtggtc gtggagccct cgggtgtggc tgccacgcgc
     2401 gatatcctgc aggcgctcac gcacgaacgg gtcgcgcacc tcgtcggcgg catccgcacg
     2461 gtcttggtgc tcgatcccga cgaccacgat tggttcgtcg agatgagccc gaccttcgtc
     2521 gattcgcaga tcggcctggc gcagttgatc ctgctcaaca agacggactt ggcagatccg
     2581 gaggacgtgg cccgcgtcac ggccgacctc gagcgccgca acccggatgc ggtcgtcttg
     2641 cccacgagcc acggagcctt ctcctggcgg gccgtcgagc acatcctccc gccgctgccg
     2701 tcgccggagg gagagtcggc gcgtcttgag ggcttcgagt cgttctcggc gcggctgccc
     2761 gaagcgttgg atgcggcctc gttgcgtggc ctcttctcgg agttgaccga cggccgcttc
     2821 ggtgacgtgc gccgagccaa gggagtgttc ctgacgggtg acggtgccgg cagcgtccgc
     2881 ttcgatctgg ccgcccgccg ggtgttcgag acggagtggc cgcgcaccgt gccgggccgc
     2941 atcaacgtca tcggccgtga tctcgaccga caagggatcg agcggtccct cgccgaagcg
     3001 gcagtgatgg cggaagcgcg gtccggggcg gaaagggatc ctcgatgacc gcgggtgacg
     3061 ccgcgcgcgg ggcagcccct tcgggcggag actccacacg agaacgggag gcaggcacag
     3121 tgcgacgcga agggtggtcc aggcggcgac gcatcctctc tttcctgggc atcgtcgggg
     3181 tggcgacgat cgttgccggt tgcgccggcc agccgccgcc ggcttccggc tcgcccggct
     3241 tcttctacgg cgtcctgcac gggttcctga tcctcgtcga cttcatcgtg gggctgttct
     3301 cggatgtacg catctacgcg taccccaatt cgggaggttg gcacgacttc gggttcctcc
     3361 tcggggcgag tatgttcttg ggagggggcg gcggcgccgg cgcccgccgg aggtgaggtg
     3421 ggatgaaaga gacgatctct atcggcgact tcactgtccg cgacgtgcgt gtcgaggacg
     3481 ccccctcgat agccgcctac gccgacaatc cccgggtcgc ggccaacctg cgcgacgtct
     3541 tcccccaccc gtaccgtctg gaagacgccc agggcttcct cgcgcacgtg atgcaacagt
     3601 tgcccccgac cgtcttcgcg atcgccaccg aggccgaggc cgtcggggtc atcgggctga
     3661 cgctcggcga agacgtccat cgcttcaccg ctgagctcgg atactggctg ggggagccgt
     3721 tctggggccg tggggtgatg acctcggcag ttcgagtggt ggttgcgcac ggattcagag
     3781 agttggggtt gctacgcatc cacgccgatc cttatgcggg caacgtaggc tccgcccggg
     3841 ttctggagaa ggccggtttc gagttggaag ggcgcatgcg cgcgaatgtt ttcaagaacg
     3901 gccgggtcct cgaccagctc ctctactcca cgattcgcga gggagtggcc tagtcgcccc
     3961 gtggcacacc gtcgtggcag ggcgtgcacg tggcagcgcc gaaaccggac gcgtgacagt
     4021 cctggcacga cagccggtgt tcgccaccgg gaatgtgctc c
//
LOCUS       DASRMJ010000067         8885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_185751, whole genome shotgun sequence.
ACCESSION   DASRMJ010000067 DASRMJ010000000
VERSION     DASRMJ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185751"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFD74_01940"
     CDS             <1..604
                     /locus_tag="VFD74_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HZK48348.1"
                     /translation="NHRQGGVFMGVGRSFTYMLQQPGGLGKLVIGGLLMFVPIIGWAI
                     VAGYMIRTLRQVSAGVDTLPEWTDWGQLLVTGLMVWAGSLIYEIPGMIIGRFGAGGTL
                     LQSLWSLVVFIVLPAALIRFAATDNFGAFFDFSEIIAFIQSNTSNYILAVIVAMVAGF
                     LAVFGLILLIVGVVFTIMWASLVMAHLYGSVWAGRVQPTS"
     gene            867..1439
                     /locus_tag="VFD74_01945"
     CDS             867..1439
                     /locus_tag="VFD74_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZK48349.1"
                     /translation="MSAKLTEIRWHARGGQGAVTAAKMVAELAVERGRHFQAFPEYGP
                     ERSGAPIVAFTRISNEPIHIYSPIEHPDVVLVLDATLVGSVDVTEGAPDDAVVIVNTD
                     QSPESIAAEMDFGSRHVFTVAASKIASETIGRAIPNTPMVGAIAKATGLFTLEEVEDH
                     LRVSFGKKFSPKVVEGNLEAVRRAYNEVQG"
     gene            1436..1756
                     /locus_tag="VFD74_01950"
     CDS             1436..1756
                     /locus_tag="VFD74_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HZK48350.1"
                     /translation="MKWDISDIKDWGPGRHEPGAVIPEAGNARAYKTGGWRTNRPVRD
                     DETCTQCLFCFFFCPDASVLLADGKVADFDLDHCKGCAICATECPVDAITMHPESEFR
                     RKAE"
     gene            1753..2952
                     /locus_tag="VFD74_01955"
     CDS             1753..2952
                     /locus_tag="VFD74_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZK48351.1"
                     /translation="MSTTTEKLLAVTGNQAVAMAMKQLDPDVVAAYPITPQTDVVQTF
                     SQYVADGVVKAEFVTVESEHSAMSACIGSAAAGARTMTATSSAGFALMWEVVYVAASL
                     RLPIIMTTVNRALSGPINIHCDHSDSMGGRDSGWIQIYNEDAQEAYDATVMSFPIAEH
                     ADVRLPLMNMYDGFIISGAIGPLRPLSDETVKAFAGEQPVVRTLLKPEEPITVGPFDG
                     LHGWYFEHKIAQNRAMEAALPIIQDTYDRFAEVSGRQYHHLMQYDMEDADVAIVVIGS
                     AAGTARTVIKTLRDEGIKAGLIKIRTFRPFPTEEMARALSGVKAVAVLDRADSFGAQG
                     GPLYLEVLAALYQKGVHTKTVNCIYGLGGRDLYPRNIEDVYRMLADAADGGDLPHSRV
                     YLNMKGE"
     gene            2954..3913
                     /locus_tag="VFD74_01960"
     CDS             2954..3913
                     /locus_tag="VFD74_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZK48352.1"
                     /translation="MATKLSPKEYIKRPDHLTGGHRLCAGCGAGIVLRQVLMGAKDNP
                     VVISNATGCLEVSTTIYPYTAWKDSFIHSAFENAAATISGVEAAYKSLLRQGKVQSDK
                     PYKFLSVGGDGGSYDIGLQALSGALERGHDIVVLCYDNGAYMNTGFQRSAATPFGAWT
                     TTSPDGKVRPGKTQQRKNLTRIAVAHGIPYVAQASPHNFRDLISKAEVAFDTPGPAFL
                     NVLSPCPRGWRSADPDGMKLAKMMVQSCFWPLYEVVDGRYTINYDPKDRKIPIRDWME
                     QQGRFKHLFKPQFAYMIDEAQAYVDSEWDRLKSMTTLPGGGEA"
     gene            3921..4703
                     /locus_tag="VFD74_01965"
     CDS             3921..4703
                     /locus_tag="VFD74_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZK48353.1"
                     /translation="MTETAGAQTAPRRSHLIVVQHHDRVPLGALAPALTLDADVTFVR
                     GYRAPAEARAQVRALIDSGGYDGMVVLGGPMGVYEAERFPFLLDSLSLLEDALRRGAP
                     ILGLCLGSQLLSHALGSPVFPGSDRGLPPEVGYFPARLTGEGVADPAATLYAEPDPVL
                     FWHRDTHDLPDGAVLLATSDRYPLAAFRWGRWTYGFQFHLEITMEWLPRWIEESPLAE
                     EAEGGAQELLRAGGRCAAVNSERATALGRQFVEHARAFREGL"
     gene            complement(4700..6328)
                     /locus_tag="VFD74_01970"
     CDS             complement(4700..6328)
                     /locus_tag="VFD74_01970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZK48354.1"
                     /translation="MEPSILPDSGGVLKDMRSSHGEVRPDPGLEAPAFDETPDLNLPD
                     EFDALGELGEVLATSDSVRARGVMDFFWTGLAPFTLLVVFGTSVVVFALTAYLLDAEL
                     PHLLALSGSVMGVGLVVLLPLEYRLVRARILGPVKRLEFELVGKSPWRPERDALLGSL
                     RRVVGEIRRALRTMDNDLRLERERGDDLQARLAERAVAEVFVDRVVEGLRGAESVSQF
                     AVEAGRLVRSVWPADDLLLLNREDRQGEFGILAWEHGGVMVDLATLEGEPPRYRLGSL
                     PVPVKEALRSGFCAEIGLPFSQDANFPQARSFVAIGLDHRGGASGVMLALSSRLDPPG
                     EEPLRRSRPLFSMGFGRAMYVRELGEAEIRDALTGAFTHDHFLTLVRYEVARANRYSR
                     PVACLMIDVDDLRRINGEHGSAAGDRVIAEVAQLVAGAIRSSDSLARISGGRLSLLLP
                     ETNTEASEVVAERVRSRVEEHPFILEKSQVERATVSVGIAVHPPLGVTAMSLVDAAER
                     ALAEAKRGGRNRVTVAAGGPVEPARPSEGHGDSG"
     gene            6488..7720
                     /locus_tag="VFD74_01975"
     CDS             6488..7720
                     /locus_tag="VFD74_01975"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZK48355.1"
                     /translation="MKFGGTSVADVACVKRVAQRIVDARRAGSPVVAVVSARGDTTDE
                     LISMAREISDAPPEREMDMLLSAGERISAALLAMAIHDLGFEAISLTGSQAGIVTDSV
                     HTKAKILDIRPTRVQKALDEGKVVLVAGFQGVSTQTHDVTTLGRGGSDTTAVALAAAL
                     GAKVCEIYTDVDGVYTADPRIVPQARKMQSISCEEMLEMAASGAKVLMLRSVEFARRY
                     GVALHVRSSFSNEEGTWIIQEEENMEQAIVSGVTYALEDAKVTLHRVPDRPGVAARVF
                     TALANAAVNVDMIIQNVSEGGSTDISFTVGEDDIDIVRSLLGELQPEFGFDALEADAD
                     MAKVSLVGAGMKSHPGVAARMFEVLAENSINIEMISTSSIKVSCVIRQQHVERAVTAL
                     HEAFGLDKDSVQRDEVFS"
     gene            complement(7814..8638)
                     /locus_tag="VFD74_01980"
     CDS             complement(7814..8638)
                     /locus_tag="VFD74_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HZK48356.1"
                     /translation="MRPETRRSSERELRSWCGRADVVAVARPRKWRCVHGPARPRMFK
                     PAGVPARELEVVVLTLDGLEALRLLDLEGLRQEEAATELGVSRSTVSRLAAEARRSVA
                     EALVGGKALVIEGGPAAVARRASREVSTTTGGESMIIAVPYDEGQVNGHFGRTEAFLI
                     ARAEGSELKESTVYDVVGLQHNHSGLAGFLASRGVEVILAGGMGPPMQQALRQAGFSL
                     YTGVSGSAAGALETFLSGTLEQSDAACSHHGEAHGHDHQGGGQCGDEHQHDGGCAH"
BASE COUNT         1597 a         3087 c         2779 g         1422 t
ORIGIN      
        1 aaaccatcgc caagggggag tcttcatggg tgtgggccgt tcgtttactt acatgcttca
       61 gcaaccggga ggactcggca agctcgtcat aggcggattg ctgatgttcg tgccgatcat
      121 cggctgggcc atcgtggccg ggtacatgat ccgcaccttg cggcaggtct ccgccggtgt
      181 ggacacactg cccgagtgga ccgattgggg acaactcttg gtcaccggtc tcatggtctg
      241 ggccggcagc ctcatctatg agatcccggg gatgataatc ggccgattcg gcgccggcgg
      301 cacgctgctc cagagcctgt ggagcctggt cgtcttcatc gtcttgccgg cggcacttat
      361 ccgcttcgcc gccaccgaca acttcggagc cttcttcgac tttagcgaga tcatcgcctt
      421 catccagtcg aacacatcca actacatcct cgcggtgatc gtggccatgg tcgccggctt
      481 cttggccgta ttcggcctga tcttgctcat agtgggagtc gtgttcacca tcatgtgggc
      541 gtcgctggtg atggcccacc tctacggatc ggtgtgggcc ggccgagtcc aaccgacgtc
      601 ctaggcggtc gagtcgggga tggggccgac cttgttggcc ccatccggcc cgtgttagac
      661 tgccattctg tgcggtcggt gaagacgggc acaccgtcgg cggtgggtag cacgcgaccg
      721 cgtcaccctg gatcgactcg ggtgccacgt atggcaccct tttcggcatc cctgggaaca
      781 cgtgggtgcg tccggacggg cagactcgtg cgggagcgac aaagaacccc ccgcaacaca
      841 atgaagtccg agtaaggagc atcgacttga gcgccaaact cacagagatc cggtggcacg
      901 cacgcggagg ccagggtgcc gtgaccgccg ccaagatggt agccgaactg gcggtggagc
      961 ggggtcgtca tttccaggcc tttccagaat acgggcccga gcgctcgggt gcgcctatcg
     1021 tcgctttcac gcgcatcagc aatgagccca tccatatcta ctcgcccatc gagcaccccg
     1081 acgtggtcct cgtgctcgac gccacactcg tcggctccgt cgacgtcacc gagggagcgc
     1141 ccgacgacgc tgtggtcata gtgaacacgg atcaatcgcc cgaatccata gccgccgaga
     1201 tggacttcgg cagccgccac gtattcaccg tggccgcctc caagatcgcc agcgagacca
     1261 tcggcagggc gatccccaac acgccgatgg tgggcgccat cgcgaaagcg accggcctgt
     1321 tcacactcga ggaggtggag gaccacctgc gcgtcagctt cggcaagaag ttctcgccca
     1381 aggtggtaga gggcaacctg gaagcggtcc gccgagccta caatgaggtc caaggatgaa
     1441 gtgggatatc tccgacatca aagactgggg gccgggacgg cacgagcccg gcgccgtcat
     1501 ccccgaggcg ggtaacgccc gtgcctacaa aaccggcggt tggcgcacta accggcccgt
     1561 ccgcgacgac gagacgtgca cgcagtgcct cttctgcttc ttcttctgcc ccgacgcctc
     1621 cgtcctgctt gccgacggca aggtggccga tttcgaccta gaccactgca agggctgcgc
     1681 gatctgcgcc acggagtgtc ctgtcgacgc gatcaccatg catcccgaga gcgagttccg
     1741 gaggaaggca gaatgagcac tacgaccgag aaactcctcg ccgtcaccgg gaaccaggcg
     1801 gtggccatgg ccatgaagca actcgacccc gacgtggtgg ccgcctaccc catcacgccg
     1861 cagaccgacg tggtgcagac cttcagccag tacgtcgccg acggcgtggt gaaagccgag
     1921 ttcgtgaccg tggagtcgga acattcggcc atgagcgcct gcatcggctc tgccgccgcg
     1981 ggcgcccgca ccatgaccgc caccagctcc gccggcttcg ccctcatgtg ggaagtcgtc
     2041 tacgtggcgg cctccctgcg cctgcccatc atcatgacca cggtgaaccg cgccctctcc
     2101 ggccccatca atatccactg cgaccactcc gactcgatgg gcggtcgcga ctcgggctgg
     2161 atccagatct acaacgaaga cgcgcaggaa gcctacgacg ccaccgtgat gtcgttcccg
     2221 atcgccgagc acgccgacgt ccgtctgccc ctcatgaaca tgtacgacgg gttcatcatc
     2281 tcgggggcca tcggccctct gcgcccgctg tccgatgaga cggtcaaagc gttcgccggg
     2341 gaacagcctg tcgtccgcac cctcctgaag cccgaagagc ccataacggt gggacccttc
     2401 gacggcctgc acggctggta tttcgagcac aagatcgccc agaaccgggc gatggaagcg
     2461 gcgcttccca tcatccagga cacctacgac cggttcgcgg aagtctccgg ccgccagtat
     2521 caccacctca tgcagtacga catggaagac gccgacgtgg ccatcgtggt gatcggctcc
     2581 gcggccggga cggcgcgaac ggtcatcaag acgctgcgcg atgaaggcat caaggcggga
     2641 ctcatcaaga tccgcacctt ccgccccttc cctaccgagg agatggcccg cgcgctctcc
     2701 ggtgtgaagg cggtcgcggt gctcgaccga gccgattcgt tcggcgcgca gggcggtccg
     2761 ctctacctcg aggtgctcgc cgccctctac cagaagggcg tgcacaccaa gaccgtgaac
     2821 tgcatctacg ggctcggagg ccgcgacctc tacccgcgga acatcgagga tgtctaccgc
     2881 atgctcgccg acgccgccga tggcggcgat ctgccccatt cccgtgtcta cctcaacatg
     2941 aagggggaat aggatggcga ccaagctctc ccccaaggag tacatcaagc gtcccgacca
     3001 cctcacgggc ggacaccgcc tctgcgcggg ttgcggcgcg ggcatcgtgc tgcggcaggt
     3061 gctgatggga gccaaggaca accccgtggt gatctccaac gccaccggct gcctggaagt
     3121 ctccaccaca atctacccgt acacggcctg gaaggactcg ttcatccata gcgccttcga
     3181 gaacgccgcc gctaccatca gcggcgtgga ggccgcctac aagagcctgc ttcgccaggg
     3241 caaggtgcag tccgacaagc cctacaagtt cctgtcggtg ggcggagacg gcggttccta
     3301 cgacatcggc ctgcaggccc tgtcgggagc tctcgagcgc ggccatgaca ttgtcgtgct
     3361 gtgctacgac aacggcgcat atatgaacac cgggttccag cgttcagccg ccactccctt
     3421 cggcgcctgg accaccacca gccccgacgg caaggtccgc ccggggaaga cgcaacagcg
     3481 caagaacctc acgcgcatcg cggtggccca cggcatcccc tacgtggcgc aggcctcccc
     3541 ccacaacttc cgtgacctta tctccaaggc ggaggtggcc ttcgacactc cgggccccgc
     3601 gttcttgaac gtgctctcac cctgcccgcg cgggtggcgt tccgcagatc cggacggcat
     3661 gaagctggcc aagatgatgg tgcagtcgtg cttctggccc ctctacgagg tggtcgacgg
     3721 ccgatacacg atcaattacg acccgaagga ccgcaagatc ccgatccggg actggatgga
     3781 gcagcagggt cgcttcaagc acctcttcaa gccccagttc gcctatatga tcgacgaggc
     3841 gcaggcctac gtcgattccg agtgggaccg tctgaagagt atgactaccc ttcccggtgg
     3901 cggagaggcc tgatcctcgg atgaccgaga ccgccggggc gcagaccgcg ccccggcggt
     3961 ctcacctcat cgtcgtccag caccacgacc gcgtgcctct gggcgccttg gcgcccgccc
     4021 tcaccctcga cgccgacgtc accttcgtcc gcggctaccg cgccccggcc gaagcccggg
     4081 cacaggtgcg cgcgctcatc gactccggcg gctacgacgg gatggtcgtc ctgggcggcc
     4141 ccatgggcgt ctacgaagcc gagcgcttcc ccttcctgct cgattccttg agcctgctcg
     4201 aagacgctct ccgccgaggc gcccccatcc tggggctgtg cctcggctcc caactcctga
     4261 gccacgcact ggggagtccg gtcttcccgg gatccgaccg ggggctcccg cccgaggtgg
     4321 gctacttccc ggcgcgcctc acaggggagg gtgtcgccga cccggccgcc accctgtacg
     4381 ccgaacctga cccggtgctc ttctggcacc gagacaccca cgacctaccc gacggcgccg
     4441 tgctcctggc gacgagcgac cgttacccat tggcggcgtt ccgatggggt cgctggacct
     4501 acgggttcca gttccatttg gagatcacca tggagtggct cccgcgttgg atcgaggagt
     4561 ctccgttggc ggaggaggca gagggcggcg cgcaggagct gctacgggcc ggcggccgct
     4621 gcgccgcggt caactcggag agggcgaccg ccttgggtcg gcagttcgtg gagcacgcac
     4681 gcgccttccg agaaggactc tagcccgagt ccccgtgccc ctcggagggc cgggcgggct
     4741 cgacaggccc gcccgcggcc accgtcacac gattccgccc gccgcgcttg gcctccgcca
     4801 acgcccgttc ggctgcatcc acgagcgaca tcgcggtcac tcccaacggc gggtggacgg
     4861 cgatgcccac gctcaccgtc gcccgctcca cctggctctt ctcgaggatg aaggggtgct
     4921 cctccacccg actgcgcacc cgttccgcga cgacctcgga cgcctcggtg ttcgtctcgg
     4981 gcagcaacag tgagaggcgc ccgccgctga tgcgggccaa actgtccgag gaacggatcg
     5041 ctccggcgac gagttgcgcc acctcagcga tcactcgatc gcccgccgcc gagccgtgct
     5101 cgccgttgat ccgtcgcagg tcgtcgacgt cgatcatgag gcaggcgacc ggtcgggagt
     5161 agcggttcgc acgggcgacc tcgtacctca ccagcgtcag gaaatggtca tgcgtgaacg
     5221 ccccggtcaa cgcgtcgcgg atctccgcct cccccagctc gcgcacgtac atcgcgcggc
     5281 cgaagcccat gctgaacagc ggacgggacc gcctaagggg ttcctcaccc ggcgggtcca
     5341 agcgcgagga cagcgccagc atgaccccgg aggcgccgcc acgatgatcg agcccgatgg
     5401 cgacgaaact ccgcgcttgg gggaagttgg cgtcttggct gaaaggcaga ccgatctcag
     5461 cacagaagcc ggagcgcaac gcctccttca cgggcacggg caacgatccc aggcggtagc
     5521 gcggcggctc gccctccaag gtcgcaaggt ccaccatgac tcccccgtgc tcccaggcca
     5581 ggatgccgaa ctcgccctgc cggtcctccc gattcaagag tagaaggtcg tccgcgggcc
     5641 agacggagcg cacaagccgg cccgcttcga cggcgaactg gctcaccgac tcggccccgc
     5701 gcaacccctc gacgacgcga tcgacgaata cttcagcgac cgcgcgctcg gccagccgag
     5761 cctgcaggtc gtcaccgcgc tcccgttcca gccgcaggtc gttgtccatc gtgcgcagcg
     5821 cccgtcggat ctcgcccaca acgcggcgca gggaaccgag tagcgcgtcc cgctcggggc
     5881 gccacggcga cttgcccacg agctcgaact ccagcctctt gaccggcccg aggatgcgtg
     5941 cccgcacgag gcgatactcg agcggcagca ggaccacgag gcccacaccc atcaccgagc
     6001 ccgacagcgc cagcagatga ggcagctccg cgtccagcag gtaggcggtg agcgcgaaca
     6061 ccaccaccga cgtgccgaaa acgacgagca gcgtgaaggg agccagaccg gtccagaaga
     6121 agtccataac tccgcgcgcg cgcaccgagt cagacgtcgc gaggacttcg cccagttcgc
     6181 cgagggcgtc gaactcatcc ggcagattaa ggtcgggcgt ctcgtcgaag gcaggggcct
     6241 cgagtcctgg atccggacgc acctcgccgt gtgacgatcg catgtctttc aaaacgcccc
     6301 cgctgtcggg tagaatgctt ggttccacct tactgtatcg tcctcttgaa cgagagggtt
     6361 gactcctttg tctacatcat cgcaacctga tccaccgatc accgcggtcg cgctcgatgc
     6421 caccgggcgc ccggtgacca tcatggaggc ccaccccgtc gcccgcgagg ccaccgagac
     6481 gatagtgatg aagttcggcg gcacgtcggt ggccgatgtc gcatgcgtga agagggtggc
     6541 gcagcgcatc gtcgacgccc gcagggcggg aagcccggtg gtagccgtgg tctcggcccg
     6601 cggcgacact acagacgagt tgatatcgat ggcgcgcgag atctccgacg cgcccccgga
     6661 gcgagagatg gacatgctcc tatcagcggg agagcgcatc tcggcggcgc ttctggccat
     6721 ggccatccac gacctcggtt tcgaggccat atcgctcaca ggctcgcagg cgggcatcgt
     6781 caccgactcc gtgcacacca aggccaagat cctcgacata cgccccaccc gtgtgcagaa
     6841 ggcgctggac gagggcaagg tcgtgttggt tgccggtttc cagggagtgt cgacccagac
     6901 ccatgacgtc accacgctgg gcaggggcgg gtcggacact acggccgtgg ccctggccgc
     6961 cgcgctgggc gccaaagtgt gcgagatcta cacggacgtc gacggtgtct acacggccga
     7021 cccgcgcatc gtgccccagg cccgcaagat gcagagcatc tcctgcgagg agatgctgga
     7081 gatggcggcg tcgggggcga aggtcctgat gctgcgaagt gtcgaattcg cgcgacgcta
     7141 cggcgtagcc ctacacgtgc gctcgagttt cagcaacgaa gagggcacgt ggatcatcca
     7201 ggaagaggag aacatggaac aggccatcgt ctcgggagtc acctacgcac tcgaggacgc
     7261 caaggtcacc ttgcaccgcg tccccgaccg gcccggtgtg gccgcgcgcg tcttcaccgc
     7321 gctggccaac gccgccgtca acgtcgacat gatcattcag aacgtcagcg aaggcggcag
     7381 cactgacatc tcgttcacgg tgggcgagga tgacatagac atcgtccgct cgctgctcgg
     7441 cgagctccag cccgagttcg ggttcgacgc cttggaggcg gatgccgata tggctaaggt
     7501 ctcgctcgtc ggtgccggga tgaagagcca ccccggcgtg gcggcccgga tgttcgaggt
     7561 gctcgccgag aacagcatca acattgagat gatctcgacg agctcgatca aggtgtcatg
     7621 cgtcatccgt cagcagcacg tcgagcgggc cgtgacagcg ttgcacgaag ccttcgggtt
     7681 ggataaggac tccgtccagc gggacgaagt cttcagctag cccgggcggc gccggcagtg
     7741 acggggtcgc gccgcgccgg cggccgagcg tcggcgccgg cagctcaccg acgccgacgc
     7801 tacactcggg gggtcagtga gcacaccctc catcgtgctg gtgttcgtct ccacactgcc
     7861 cgccgccctg atgatcgtga ccgtgtgcct ctccgtggtg gctgcacgcg gcatcggact
     7921 gctccaacgt gccggagagg aaggtctcca aagcccccgc agccgagccc gacaccccgg
     7981 tgtagaggga gaatcctgcc tgccgcagcg cttgttgcat gggcggaccc atacccccgg
     8041 ccaggatcac ctcgactcct cgagaagcga ggaatccggc gagcccgctg tggttgtgct
     8101 ggagacccac cacgtcgtac acggtgctct ccttgagctc ggaaccttcg gcgcgcgcga
     8161 tcagaaaggc ctcggtgcgc ccaaagtgac cgttcacctg tccttcatcg tacggaacgg
     8221 cgatgatcat gctctctcct ccagttgtgg ttgagacctc acgcgatgcc cggcgcgcaa
     8281 ccgctgccgg gcctccctcg atgacaagag ccttgccccc gacgagcgcc tcagcgacgc
     8341 tccgtcgcgc ctcggcggcc agacgactca cagtggaccg cgacaccccc aattccgtcg
     8401 cggcctcttc ctgcctgaga ccctcgagat ccaggagtcg cagcgcctcg aggccgtcga
     8461 gggtcaggac cacgacctcc aactctcgcg ccggcacacc ggcgggtttg aacatccgag
     8521 gccgagccgg accatgaacg cagcgccact tgcgagggcg tgccactgct acgacatccg
     8581 cccgcccgca ccaagagcga agctctcgct cggacgatcg acgtgtctcg ggtcgcatat
     8641 aatgcacata cgtgcattat aacagactcg atcccaaccc cccgggttga caaaagcatg
     8701 tgagcgcggt tacctgtcga gaccgaacaa gtcacaagac ggccagcgcc ccacctcgac
     8761 gcgcggagga acatgaacac ccaacccaga gacctctatg cggacctcga gcacctcgtc
     8821 tctcaccgca cgaccaggga cgaactggcg gccggtatcc ccatcatcga gcggggtgtg
     8881 ggctg
//
LOCUS       DASRMJ010000068         6293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_476935, whole genome shotgun sequence.
ACCESSION   DASRMJ010000068 DASRMJ010000000
VERSION     DASRMJ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476935"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFD74_01985"
     CDS             <1..463
                     /locus_tag="VFD74_01985"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HZK48357.1"
                     /translation="RILEAGVIGWLAEKGVVTVAAGGGGVPVVEQADGSLVGVDAVVD
                     KDRASALLGLQAGAERLLVLTSVDAVYESFGTPTQKRLAHLSVDRAARLLAQGQFPSG
                     SMGPKIQAVIAFLEGGGGEARIGLPERLEGILSGEEGTLISRGHVASLDGR"
     gene            complement(515..1375)
                     /locus_tag="VFD74_01990"
     CDS             complement(515..1375)
                     /locus_tag="VFD74_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZK48358.1"
                     /translation="MALNGRRVAILAAQQYEDLELWYPKLRLEEIGVAVVVAGLGERS
                     YASKHGYPADTDAVIDDLDPDAFDGVIIPGGWAPDYLRRSEAVKRFVAAVAGRGGLVA
                     AICHGGSVLVSAGVANGRRLTSVPSIKDDMVNAGAVWIDAPVVVDGSLVTSRNPRDLT
                     SFMPAVIAVLEERSHQAEQAQAAARSSVDEQLISISLSPESIDYMLEMLVRMPAAKKY
                     RGDTTIGDHDLTTLVQDFALHSDPRGALEAEAPTIADVRSSGTTFVARGNLRALHVMQ
                     GADVPGVAAT"
     gene            1587..2171
                     /locus_tag="VFD74_01995"
     CDS             1587..2171
                     /locus_tag="VFD74_01995"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011933640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZK48359.1"
                     /translation="MAFTLPDLPYAKDALEPHISEETLEYHHGKHHNAYVTNLNGLIT
                     GTDMADKSLEEIVGAAQGGVFNNAAQVWNHSFYWKCMKPAGGGSPAGALAAAIDRDFG
                     SFDTFKEQFTKAGVTLFGSGWVWLIVDQGTLKITQTSNADLPLKHGQTALLTMDVWEH
                     AYYVDYRNARPKYIETFLTELVDWGFVAANFAAA"
     gene            2475..3713
                     /locus_tag="VFD74_02000"
     CDS             2475..3713
                     /locus_tag="VFD74_02000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK48360.1"
                     /translation="MSEKGLSYRQMSVLTAPTVGLSVCLSLVATYLPLLLSRYTNSAT
                     LIGVAIGGEGLFALAIPLVVGSMSDRVWTRWGRRRPFMIVGAPVMAAAIFLAPFQPGY
                     FSIALTTFVFFAAYHLYSAPYQALIPDLSPLHQQGRVQGFQTMMRGAGMFLGMVVAGY
                     LFESWEPLPFILGAFLMVGVTYITVSSIEEKPTPVDAPDAVAGLWSSLVDIWHTTRRE
                     KTITRLLGANFLWESTIQGLRPFIMLYFLYTLGTTTRMGATLLGLVGIVYIVAGLSGG
                     FLADRYSRERILWTGLWIYLVGCSIGFFVRDVRLGLVLLPLFGLGGSLVLTLPYAVLM
                     PLMPRDRIGQFTGLFSVSRGAATVAAPVIFGAGIDAATAVLGRASGYAIIWPLAAVAI
                     LVSLVLYRAVTSSQRAVADT"
     gene            3820..4146
                     /locus_tag="VFD74_02005"
     CDS             3820..4146
                     /locus_tag="VFD74_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK48361.1"
                     /translation="MRSTPSSPESLPTTGISAAGGSDQDLRSPLSEDATERGPQFTAA
                     ESRVLTALSEEPRLSFAVLRSRSALPPEELRTALDSLRARGLVTRLHTLGESYSSRFP
                     GLRVDE"
     gene            4133..5053
                     /locus_tag="VFD74_02010"
     CDS             4133..5053
                     /locus_tag="VFD74_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZK48362.1"
                     /translation="MSTSDPPPGEPLARRLLAVPGDVYRGIARDDCLGLAAQIAYYGL
                     FSLFPFLLFLRSLVRHLPGKQQVIQSTLDGLAALVTPESRLYEIVRDNIVEQLNAGSP
                     TLLSVGAILTLWGASSAFSVLIKAINRAYGVGETRSWQRRRLLALGLTLLAAVLVPAG
                     VSLLLLGPRLGDLVAGATSGGALHLAWVILRWPAVFALLVVPMGIIYSVAPNALQKWE
                     WVAPGAFFAAAGIIASSLGFSWFLGLNLFELRWFTYGAIGMVIVILFWMYLVGLAVLV
                     GAEVNDAVGRALRRPPRANARSQASKVAER"
     gene            complement(5146..5808)
                     /gene="hypB"
                     /locus_tag="VFD74_02015"
     CDS             complement(5146..5808)
                     /gene="hypB"
                     /locus_tag="VFD74_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016422184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HZK48363.1"
                     /translation="MPLHHDLMAANDQHAEAIRSLLGGHRIFAVNLMSSPGSGKTALL
                     EATLAAVGSRLRVAVIEGDIETCADADRLAPFGIQVVQVNTAPFGGDCHLAAPLVESA
                     VDKLDLRALDVLIVENVGNLVCPAEFDIGEDAKVVLLSVTEGEDKPLKYPLMFHEAAL
                     ALVTKTDLLPHLDVDTDLIRANMRRVNPALQVMFLSSRTGEGLDAWLSWLADGVARKK
                     AH"
     gene            complement(5942..>6293)
                     /locus_tag="VFD74_02020"
     CDS             complement(5942..>6293)
                     /locus_tag="VFD74_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HZK48364.1"
                     /translation="HEMTLAANVVEEVERCLVDLAPGARVTSVTLEVGALRAVVPEAL
                     SFCFEVASSGTRVEGARLVIQEIPVEVHCDRCERQWVIHEIGFLCPACDGTVRLLRGK
                     ELLLRAIEVDDGLD"
BASE COUNT          960 a         1992 c         2186 g         1155 t
ORIGIN      
        1 gcggatcctt gaggccggcg tcatcggatg gctggcggag aaaggcgtgg tgacggtggc
       61 agcgggcggc ggtggggtcc cagtggtgga gcaggccgac ggatcgctcg tgggtgtcga
      121 tgcagtcgtg gacaaggatc gagcgtccgc ccttctcggg cttcaggcgg gcgcggaacg
      181 cctgctggtg ctcacttcgg tggatgcggt ctacgagtcg ttcgggactc ccacgcagaa
      241 gaggctggcc cacctgtccg tcgatcgagc ggcccgcctg ctggcgcagg ggcagttccc
      301 ttcgggaagt atggggccga agatacaggc ggtgatcgcc ttcctggaag gcggcggtgg
      361 ggaagcccgc atcggtctcc ccgagcggct ggaaggtatc ctttcgggcg aagagggcac
      421 cctgatctcg cgcgggcatg tagccagcct cgatgggaga tgagccgaaa cggccggtcg
      481 tcccgccctg cctcggcccg gagcctggct acactcaggt cgcggccact ccgggcacat
      541 cggccccctg catcacgtgg agtgcgcgca ggttgccgcg cgccacgaag gtggtgcccg
      601 aagagcgcac gtcggcgatg gtgggcgcct ccgcctccag tgctccgcgc gggtcggagt
      661 ggagggcgaa gtcttggacg agcgtggtca ggtcatggtc gccgatggtc gtgtccccac
      721 ggtacttctt ggccgcaggc atcctcacca gcatctcgag catgtagtcg atcgactcgg
      781 gcgagaggga gatcgagatc agctgttcgt cgacgctgct ccttgcggct gcctgcgctt
      841 gctccgcctg atgggaccgc tcctccagca ccgcgatgac tgcgggcatg aaggacgtga
      901 gatcacgcgg gttcctggac gtgaccagcg agccgtcgac gacgacggga gcgtcgatcc
      961 agaccgctcc ggcgttgacc atgtcatcct tgatcgaggg aacggacgtg agtcgccgtc
     1021 cgttcgcgac cccggcggag acgagcacgg aacccccgtg gcagatcgct gcgaccaggc
     1081 ctccgcgacc ggccactgcg gccacgaagc gctttaccgc ctccgagcgt cggaggtagt
     1141 cgggcgccca gcctccgggg atgatcacgc cgtcgaatgc atcggggtcg agatcgtcga
     1201 tgaccgcgtc ggtgtccgcg gggtagccgt gcttggacgc gtacgagcgc tcgccgagtc
     1261 cggccacgac gacggccaca ccgatctcct ccagccgcag cttcggatac cacagctcca
     1321 ggtcttcgta ctgttgcgcg gcgaggatcg cgactcgccg tccgttcagg gccatgaggt
     1381 cctccttggt gtcgtcgggt ggggtgtctc ttcccggctg cgggtccgcc gcaaactcgg
     1441 gtgcccggtt ctcgcacatg ggtttggtgc gaagggcggg acgggtaacg aaggtgagtc
     1501 gtggccgatg gctcgaacgc cgcgccgctc gcggcctcgg ggatctgctt cggcggaaca
     1561 aacaaacgga aggagagagg acgatcatgg cattcacgtt gcccgatcta ccgtatgcaa
     1621 aggatgctct cgagccgcac atcagcgagg agacgcttga gtatcaccac ggaaagcacc
     1681 acaacgcgta cgtgaccaac ctgaacggtc tcatcaccgg cacggacatg gccgacaaga
     1741 gcctggaaga gatcgtcggc gcagctcaag ggggcgtgtt caacaatgcg gcgcaggtgt
     1801 ggaaccacag cttctactgg aagtgcatga agccggcggg gggcggatct cccgctggcg
     1861 cccttgccgc ggccatcgat cgtgacttcg gctcgttcga caccttcaag gagcagttca
     1921 ccaaggccgg tgtgaccctc ttcggctcgg gctgggtctg gctcatcgtg gaccaaggca
     1981 ctctcaagat cacccagacg tccaatgccg atctccctct gaagcacggc cagacagcgc
     2041 tgctgactat ggatgtgtgg gagcacgcgt actacgtcga ctaccgcaat gcacggccca
     2101 agtacatcga gaccttcctc acggagctgg ttgactgggg gttcgtcgcg gccaacttcg
     2161 cggcggcgta gggtcgtatc tcgcagggtt cttcggggcg gaggccggcg gcaccgggca
     2221 ggtgccgccg gcctccgcgt tgcgcacccg cacccgcacc cggccctggg gacgccgggt
     2281 gtaggtgccg tcacgtgggc cccttcgggt cggtctcctg ctagaatccc ccgcgtgacc
     2341 tatgggtccg cggggcagtg cgggctggaa ccgacgggag agtagcgcga agatccagat
     2401 ccacagagag ttcaggcgcc gccgttggcg tagccgccgc agggcttggg cggcgcgacc
     2461 gtgagtgtgg atctgtgagc gagaaggggc tgagctaccg gcagatgtcg gtgctgaccg
     2521 cacccaccgt cggtctctcg gtgtgcctgt ccctggtcgc cacatacctg ccgctgctac
     2581 tcagccgcta caccaactcc gcgacactca tcggcgtggc cattggcggc gaaggcctgt
     2641 tcgccctggc gatccctctg gtggtcgggt cgatgagcga ccgagtgtgg acgagatggg
     2701 gaaggcggcg acccttcatg atcgtcggtg cgcccgtcat ggccgccgcc atcttcctgg
     2761 ccccgttcca gccgggctac ttctcgatcg cactcacaac gttcgtcttc ttcgccgcgt
     2821 accacctgta ctcggcgccc taccaggcgt tgatccccga cctgtcgcca ctgcatcaac
     2881 aggggcgcgt gcagggattc cagacgatga tgcgcggagc agggatgttc ttgggcatgg
     2941 tggtggccgg gtatctgttc gagagttggg aaccgctgcc gttcatcctg ggggcgttcc
     3001 tcatggtagg ggtcacctac atcacggtgt cgtccatcga ggagaagccc acgcccgtcg
     3061 atgcgcccga cgcggtcgcg gggttgtggt cgagtctggt agatatctgg catacgacca
     3121 ggcgcgagaa gacgatcacc cggctcctgg gcgcgaactt cctatgggag tcgaccatcc
     3181 agggattgcg tcccttcatc atgctctact tcctctacac cttggggacc actaccagga
     3241 tgggcgccac cctgctgggg ctggtgggca tcgtgtacat cgtagcgggg ttgagcggtg
     3301 gcttcctggc agaccgctac agtagagagc ggatcctgtg gacggggctg tggatctacc
     3361 tcgtcgggtg ttcgatcggc ttcttcgtcc gcgacgtccg tctcgggctg gtgctcctgc
     3421 cactcttcgg cctgggcggc tccttggtgc tcactctccc ttatgcggtc ttgatgccgt
     3481 tgatgccgcg ggaccgcatc ggccagttca cgggcttgtt ctcggtgtca cgcggggcgg
     3541 ctacggtcgc ggccccggtg atcttcgggg ccggcatcga cgcggccacg gcggtgctgg
     3601 gcagggcttc agggtacgcg atcatctggc cgcttgcggc ggtggcgatc ctggtatcac
     3661 tcgtcctgta ccgggcggtg acatcctccc agcgggcggt agcggatacc taggagtcgc
     3721 cggcgccgct tgtttcgaga ggcggcatgg gggggaatgt agacgggact tcggaaggcg
     3781 ccacagccgc cttcgcacca cagagccgga aggtgtgttg tgagatctac cccgtccagt
     3841 cccgagagcc tcccgactac ggggatcagc gccgccggag gatccgacca ggaccttcga
     3901 agccccctct ctgaggacgc gaccgagcgt ggcccccagt tcaccgccgc cgaatcacgg
     3961 gtcttgaccg cactctcgga agaaccgcga ctctcgttcg cggtcctgcg ctcccgctcg
     4021 gcgctgccgc ctgaagagct tcgcacggcg ctcgacagtc tcagggctcg gggattggtc
     4081 acccgcctgc acactctggg tgagagctac tcatcgcgct tcccggggct ccgtgtcgac
     4141 gagtgatccg ccacccggcg aaccgctcgc gcggcgtctt ctggccgtgc cgggcgacgt
     4201 gtaccgcggc atcgcccgag acgattgcct cggcctcgcg gcccagatag cctactacgg
     4261 cctgttttcg ctcttcccgt tcctgctctt cctgcggtcc ttggtgcggc atctcccagg
     4321 aaagcagcag gtgatccagt cgaccttgga cggattggcc gccctcgtga ctcccgagag
     4381 ccgactctac gagatcgtgc gggacaacat cgtggagcaa ctcaacgcgg gcagcccgac
     4441 cctcctctcg gtgggcgcga tcctcactct atggggagcc tcgagtgctt tctctgtgct
     4501 catcaaggct atcaatcggg cgtatggcgt gggagagacg cggtcgtggc agaggaggcg
     4561 gctcctggcg ctcgggctca ctctcctggc ggccgtgctg gtgcccgctg gggtgtcgct
     4621 gctgctgctc gggccgcgcc tgggcgatct ggtcgcagga gccacgtccg ggggcgcgct
     4681 tcacctcgcg tgggtgatcc tacgctggcc ggcggtgttc gccctcctgg ttgtgccgat
     4741 ggggatcatc tacagtgtgg cccccaatgc tctgcagaag tgggaatggg tggcgcccgg
     4801 agcgttcttc gcggcggcgg ggatcatagc gtcgtcgctc gggttctcct ggttcttggg
     4861 actcaacctc ttcgagttga ggtggttcac ctacggcgct atcggcatgg tcatcgtcat
     4921 tctcttctgg atgtatctgg tgggcctggc cgtgctggtg ggtgcggagg tcaatgacgc
     4981 ggtcggcagg gcgttgcgcc gcccgccgcg cgccaatgcc agatcgcagg cttcgaaggt
     5041 agcagagcgc tagccgccgg cctcgaggcg ggcggcatgg gcaggatggg cggattgggg
     5101 aggacgggca gcctgggcag ggtggccact gcctcgatgg ctgcgtcagt gggctttctt
     5161 gcgcgcgacg ccgtctgcga gccaagagag ccacgcatcc aggccctctc ctgtgcggga
     5221 cgagaggaac atcacctgaa gcgccgggtt cactcggcgc atgttggcgc gaatgaggtc
     5281 ggtgtcgacg tccaggtggg gaagcaggtc ggtcttggtg accaacgcca aggcggcctc
     5341 gtggaacatc agtgggtact tgaggggctt gtcctcgccc tcggtcaccg atagcagcac
     5401 gaccttggcg tcttctccga tgtcgaactc ggcgggacac acgaggttgc ccacgttctc
     5461 cacgatcagg acgtcgaggg cacgcaggtc cagcttgtcg acggcgctct cgaccagggg
     5521 cgctgccagg tggcagtccc caccaaaggg cgccgtgttg acctggacca cctggatgcc
     5581 gaagggagcg aggcggtcgg cgtcggcgca ggtctcgatg tcgccttcga tgacagccac
     5641 gcgcaggcgg ctacctacgg cggcgagggt ggcttcgagg agggccgtct tgccggaccc
     5701 cggggaactc ataaggttga ctgcgaatat gcggtgccca ccgaggagag agcggatggc
     5761 ctccgcgtgc tgatcgttcg cggccatgag gtcgtggtga agcgggatct cggtggggcc
     5821 gcgtccaccc ggtccgtgcg catgtccgcc tcgatggtgc ctactgtgca ttccggccgg
     5881 tcggggcagg cgcccgctcc ggtgggggga tccggcggca ccttctggcg gtgtggggtc
     5941 gtcagtccag gccatcgtcg acctcgattg cccgtagcag gagttccttg ccgcgaagca
     6001 ggcgcacggt tccgtcgcag gccgggcaga ggaacccgat ctcgtgaatc acccactgtc
     6061 gctcgcaccg atcgcagtgc acttccaccg ggatctcctg gatgaccaga cgagcgcctt
     6121 cgacacgcgt gccggagctc gcgacctcga aacagaagga gagcgcttcc gggaccaccg
     6181 cccgcagtgc gccgacttcg agagtaaccg aggtgaccct cgcgccgggg gcgaggtcaa
     6241 cgaggcagcg ttctacctct tcgaccacat tggcggccaa cgtcatctcg tgc
//
LOCUS       DASRMJ010000069         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_222420, whole genome shotgun sequence.
ACCESSION   DASRMJ010000069 DASRMJ010000000
VERSION     DASRMJ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222420"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..869)
                     /locus_tag="VFD74_02025"
     CDS             complement(195..869)
                     /locus_tag="VFD74_02025"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZK48365.1"
                     /translation="MARARSTEETRRGEIVDAALRLILKNGFHNTTLDQVAVQAKVSK
                     GLVSYYFPKKDELFLAVLQLMIERLRSDLESAYRAHLPARERLRLNFRNLFDNGKRTR
                     QYYTVLIDFLGQALRERAVQGYTEVIYETVLTYVEWTITDGIRSGEFQEVDAHKVACM
                     VVGMMEGLVLQWLFSRDSVELGDAYAMCERLVDDLLTIPPTNGSAAVQVGDAAPSDTV
                     VSAAAR"
     gene            complement(974..1885)
                     /locus_tag="VFD74_02030"
     CDS             complement(974..1885)
                     /locus_tag="VFD74_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZK48366.1"
                     /translation="MRTPAKRTYDFGEEKVFNSGHNACPGCGEALAVRYVLNTMGPRT
                     IGVVPPSCIAIISGPQPYSSMRIPVYQTTLEASAASAAGIKRALKRQGKDDVNVLVMA
                     GDGGTYDIGFQALSGAAERNEDMVYVCLDNEGYMNTGAQKSSSTPYLAVTSSTPGGKP
                     SEKKDIAEIMAAHRIPYVATATVGYPDDLARKVAKARDMKGMRFIVVLTPCLDGWGVA
                     DDGAVRVSRLAVDTGFFPLYEVEDGERYTINHGPKGIPVERYLELQKRYRHLSESQTA
                     EIKAGVDHRWESLQARARNMAGRSTTP"
     gene            complement(1891..3225)
                     /locus_tag="VFD74_02035"
     CDS             complement(1891..3225)
                     /locus_tag="VFD74_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZK48367.1"
                     /translation="MSTTSKQGATSKQGATSTPSTTSAATAAQATAAATAATATSAAQ
                     AATPTPRNSPPANQVQLAGDTRLLLSGNHAVSYGAMRARPEVVPIYPITPQTPIAEKI
                     SDLQLAGDFDVDLMTAESEHSAMSACITASLTGVRVFTATASQGLMLMHEMLHYASGA
                     RAPIVMVNVNRTIGSPWGFWPDQTDSLAQRDTGWIQYYTENGQESLDTVIQAFKVVEE
                     LLVPAMVIHEAFYISHALEPVTVPSQERVDAYLPPFDPPHRFDPDIGESWGNVVNQEM
                     FCRHRKDLGDAMDRVVEASVRADAEYREMMGRGYGILEEYRTEDAEMLIVAFGAMVGS
                     ARVAVDMLRDAGRPIGLLKIKLFRPFPIEAVRRAVAGVPRLVVMERNFSPGVGGVLHQ
                     ELKAALYGMPDPPSIHGYLAGVGGLNVSPKKIVELVDKALTEEPVPGPVWQR"
BASE COUNT          527 a         1104 c         1122 g          574 t
ORIGIN      
        1 tacgagtata catggcagca ttcgtggagg ctccgaccgg ccgccatccg gccgctggcc
       61 gcggcggaga ccccgccggc cgccaggcgc gacccacctg gccgtgcggg ccggggtgga
      121 ggccccgacc ggccggcctc cggcctgcgc cccggggtgg aggccccgct caccgcgggg
      181 tgaccgacct cggcctagcg ggctgcggcg gagacgaccg tgtcagacgg ggcggcatcc
      241 ccgacctgaa ccgcggccga tccgttcgtg ggcgggatcg tgaggaggtc gtccaccaga
      301 cgttcgcaca tggcatacgc gtcgcccaac tccaccgagt cgcgactgaa gagccactgg
      361 agcacgagcc cttccatcat ccccacgacc atgcaggcca ccttgtgtgc gtcgacctcc
      421 tggaactcgc cggagcggat accgtcggtg atggtccact ccacgtaggt gagcacggtc
      481 tcgtagatga cctccgtgta cccctgcaca gcgcgctcac gcagggcctg ccccaggaag
      541 tcgatgagca ccgtgtagta ctggcgcgtg cgcttcccgt tgtcgaacag gttgcggaag
      601 ttgaggcgga gccgttctct cgcgggcagg tgggcgcgat aggccgactc caggtcgctg
      661 cgcagccgct cgatcatcag ttggagcacg gccaggaaga gctcatcctt cttcgggaag
      721 tagtatgaga ccaggccctt gctcaccttc gcctgcaccg cgacctgatc gagggtggtg
      781 ttgtggaagc cgttcttcag gatgagacgc agcgcagcgt cgacgatctc gcctcgcctc
      841 gtctcctccg tgcttcgtgc ccgcgccatc tagctggtcc cccctgtggc ctttccatgg
      901 aagtcggcga tcccttcaga ctgaccgacc gttcagagaa gaatacaggc agcgaaggcg
      961 cttggccaga cggtcagggc gtcgtcgacc gaccggccat attgcgggct ctggcctgca
     1021 gcgactccca cctgtgatcc acacccgcct tgatctcagc cgtctgcgac tccgagaggt
     1081 gacggtagcg cttctgcaac tcgagatagc gctccacggg gatgcctttg gggccgtggt
     1141 tgatcgtgta gcgctccccg tcttcgacct cgtagagggg gaagaagccg gtatccaccg
     1201 ccagccgtga cacccgcacg gcgccgtcgt cggccacgcc ccagccatcg aggcaggggg
     1261 tgagcaccac gatgaaacgc atgcccttca tgtcacgggc cttggccacc ttgcgtgcca
     1321 agtcgtcggg gtagcccacg gtggccgtgg ccacgtaggg gatacggtgg gcggccatga
     1381 tctcggcgat gtccttcttc tcgctgggct tgccgcccgg cgtggagctg gtgaccgcca
     1441 ggtagggggt cgacgaactc ttctgggccc cggtgttcat gtagccctcg ttgtcgaggc
     1501 acacgtagac catgtcctca ttgcgctcgg ccgcgccgga gagggcctgg aagccgatgt
     1561 cgtaggtgcc gccgtcgccg gccatcacca ggacgttcac gtcgtccttg ccctggcgct
     1621 tcagggcccg cttgatgccg gcggccgaag ccgcgctggc ttcgagcgtc gtctggtaca
     1681 ccgggatgcg catcgagctg tacggctgcg gcccggagat gatggcgatg cacgagggag
     1741 gcacgacgcc gatcgtacgc gggcccatgg tgttcagcac gtagcgcacc gccagggcct
     1801 cgccacaacc gggacaggcg ttgtgcccgg agttgaacac cttctcttcg ccgaagtcgt
     1861 aggtgcgctt cgcgggagtc ctcatcgtca tcacctttgc cacacggggc ccggaacggg
     1921 ctcctcggtc agcgccttgt ccaccaactc cacgatcttc ttgggagaga cattcaggcc
     1981 gcccaccccg gccaggtagc cgtggatgga gggcgggtcg ggcatgccgt agagggcggc
     2041 tttcagttcc tgatgaagca ccccgcccac gccgggggag aagttgcgct ccatgaccac
     2101 caggcgcggc acacccgcca cggcgcgccg cacggcctcg atggggaacg gacggaagag
     2161 cttgatcttg agcaggccga tcgggcgacc ggcgtcccgt aacatatcca ccgccacccg
     2221 cgctgagccc accatggcgc cgaaggcaac gatcagcatc tcggcgtctt cggtgcggta
     2281 ctcctcgagg atgccgtagc cgcgacccat catctcccgg tattccgcgt cggcacgcac
     2341 ggacgcttcg accacgcggt ccatggcgtc acccagatcc ttgcggtgac ggcagaacat
     2401 ctcctggttc accacgttgc cccacgactc cccgatatcg gggtcgaacc tatgaggcgg
     2461 atcgaagggc ggcagatagg cgtccacgcg ttcctgggag ggcacggtca ccggctccaa
     2521 ggcgtgtgag atgtagaagg cctcgtggat caccatggcc gggaccagaa gctcctcaac
     2581 cactttgaag gcctggatca ccgtgtcgag cgactcctgg ccgttctccg tgtagtactg
     2641 gatccagccg gtgtcgcgtt gagccaggct gtcggtctga tcgggccaga agccccaggg
     2701 cgatccgatg gtgcggttca cgttcaccat cacgatgggc gccctcgccc ccgaggcgta
     2761 gtggagcatc tcgtgcatga gcatgagtcc ctgagaggcg gtagccgtga acacgcgcac
     2821 cccggtgagc gaggccgtga tgcaggcgga catggctgag tgctcggact ccgcggtcat
     2881 caggtccacg tcgaagtctc ccgccaactg gagatcactg atcttctcgg cgatcggcgt
     2941 ctggggagtg atggggtaga tgggcaccac ttcggggcga gcgcgcatgg cgccgtagga
     3001 cacggcgtgg ttgcccgaca gcaggaggcg cgtgtcaccg gccagctgca cctggttggc
     3061 cggaggggag ttcctcgggg tcggcgtcgc agcctgcgcg gcgctcgtgg cggtcgcggc
     3121 ggtcgcggcg gccgtggcct gcgcggcggt tgcggcgctc gtggtgctcg gcgtgctcgt
     3181 ggcaccctgc ttgctcgtgg caccctgctt gctcgtggtg ctcatcgcgt cacctgctcg
     3241 agtcggacgg caccgcgcgg acactcggtc acgcagagcc cgcacccttt acagtactgg
     3301 tcgaggacga aatagaaggg gccggag
//
LOCUS       DASRMJ010000070         7218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_442362, whole genome shotgun sequence.
ACCESSION   DASRMJ010000070 DASRMJ010000000
VERSION     DASRMJ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442362"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFD74_02040"
     CDS             complement(<1..206)
                     /locus_tag="VFD74_02040"
                     /inference="COORDINATES: protein motif:HMM:NF025664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HZK48368.1"
                     /translation="MPQTPLGGALIDRVLAGDALGAVLERVPRLPTLMVDREAEISFE
                     MIATGVFSPLVGAPSKEENESIVET"
     gene            complement(479..1219)
                     /locus_tag="VFD74_02045"
     CDS             complement(479..1219)
                     /locus_tag="VFD74_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZK48369.1"
                     /translation="MKETYRALIVDDEAPARSELRFLLNRHSRIKVVGEASGAEEALA
                     LAENLSYDVVFLDINLPDMDGMQVATRLAGGADNPYVVFVTAYSEFAVRAFEVSAFDY
                     LVKPVTERRIDQTVDRLLSILDVTSRAAKERPARLERLAVNRSDKTVLLDLHRIYYFQ
                     AEGDYTRVVAQGGSYLASHSLRSLAERLDPKEFFRCHRSYIVNLSHVSEIISLPSNTF
                     GLRLADDRKTLLPLSRRQTKELRNRLEF"
     gene            complement(1216..1653)
                     /locus_tag="VFD74_02050"
     CDS             complement(1216..1653)
                     /locus_tag="VFD74_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK48370.1"
                     /translation="MMQHQDTGTAASLDLRDAVETPIVLSIPARPEYVALSRLTIAAL
                     ATRFGLDQEAASDLKLAVTEACSLFIRDAAVAPEPSIITVEFHLAEERWSISVAGTVG
                     PNGLSGGDHPAGLSLVVIQALTDEVETQQEGSRGRLRFAKSVR"
     gene            complement(1892..2929)
                     /gene="amrS"
                     /locus_tag="VFD74_02055"
     CDS             complement(1892..2929)
                     /gene="amrS"
                     /locus_tag="VFD74_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZK48371.1"
                     /translation="MSPEAVLWEPLAAGSARCTVCPHRCVIRNGGVGLCRVRGVTDGR
                     MQSLVYGRPATVVSDEIEKKQLYHFHPGTRALSLGTLGCNVLCAGCPNWQISHAGAGA
                     GEFSRLPTLLPDEAVAMARKHKLAGVAWTYNDPAVWIEYVHDVAMAFREAGLYTAFVT
                     AGYLSSEALDYVAPYLDAFKFDLKAPGVDGWSRLTKVKDPAPAMEAAVRAVRLHGCHV
                     EVVSNIVPGVNDDDESLNAMAAWVVYNLGVKTPWHVTRFLPDFELNYLKPTPIAVLER
                     AAAFGRSAGLEFVYVGNVPGHASRNTNCPACGRTAIRRGDRGVEQAWVRDGRCASCGE
                     DLRVVMGSTSA"
     gene            complement(2926..4089)
                     /locus_tag="VFD74_02060"
     CDS             complement(2926..4089)
                     /locus_tag="VFD74_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZK48372.1"
                     /translation="MTVTGDGGRAAAGAAAAEPPTLDVPGWLRALVDGLASGLREVVL
                     PSLGSAFSRGLTGRAAGGDTTFAIDELAEDYLASYLTQRDLPVAVYSEDRGLVRFGGS
                     TPEYVLIVDPIDGTRPAAAGFEAACVSVAAARASDRPVMSDVVYGVVAEIKEGGVFSA
                     VRGGGARITTPDGSERCVSISSNTDLTRLFWTIGFRGRPALELATALGGLIDLSSVDG
                     AVFDIGSATYSMTRILTGQLDAYVDVGPRMIELAPWVEARFREVGKGAVLNNSPHDVA
                     ASTLILREAGCIVTDAAGRSLDDRPLLGSDMSHQMSVLACASPALHEAVLAEVARGID
                     VLVGAGRSAAGAAAAGKAAARAAATGVSAAGLEVAPEGPHRFSAGPYVGSEAS"
     gene            complement(4086..4787)
                     /locus_tag="VFD74_02065"
     CDS             complement(4086..4787)
                     /locus_tag="VFD74_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HZK48373.1"
                     /translation="MIDSTRMRSEKRASGSSAALFAAPNFVYWGGPNLYVNLTSRCSA
                     SCTFCLASFTDEIYGYDLSLAVGDEPEVPDLVHAFELAFLDGAPEEVVFTGLGEPTLQ
                     LDVILGSVEWLGTRRLPTRLDTNGHAALLHPGRDVVRELTDSGLRAASVSLNAHDEFT
                     YDLLCRPTFSKAYRAVLRFIRECVDAGLDVTASVVDLPGVDLGAAASVAADLGAAFRV
                     RRPVLPPDRRREGTT"
     gene            5004..5870
                     /locus_tag="VFD74_02070"
     CDS             5004..5870
                     /locus_tag="VFD74_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK48374.1"
                     /translation="MKRSWLIVLMLLVVALAVLPLTAGCGGDKETTTTTAAAAGDPVA
                     AADAAIAAITKSDAIEMPPTLEAGKLQAGSDTSFAPFEFSDEKGGYIGFDVDLVTAIA
                     KKIGLEAAVVATAWDGIIPNLVADRYDIIMSAMTITEERQQQIDFTDGYIDTNLAITT
                     KVDAPIADAAGLEGKIVGVQVDTTGQFEVEKVAGVKEIKKYENSIAAIQDLLAGRIDA
                     VVNDSPVNAYYTRDKPEFGNTGKIGSDDKYGYGIKKESSVLRDAMNAALAELKADGTY
                     DLIYAKWFGGDK"
     gene            6073..6882
                     /locus_tag="VFD74_02075"
     CDS             6073..6882
                     /locus_tag="VFD74_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZK48375.1"
                     /translation="MESLLNGFEAFQRYYLNWQIIKETTPLLLKTLPIVGELLVLSMF
                     FAFLLGLILSLMRLSRVRVFRWFAGAYVDFFRGLPLLLNFVFVYSGLAIVGAASGLRF
                     LILPSIPAAVLSLSTTYAAYSAEVFRAGIESIHRGQMEAARSLGMTYPQSMRYVVLPQ
                     AIKVVIPPLTNEFIALIKDTSLASVISVQELLFTAKEKMGVVANPTPLMVAAFVYVLF
                     TIPLIRLASRLEKKREGSSKGAKPTDGSTGKTSAPVLTDVATQLPKGGGLG"
     gene            6894..>7218
                     /locus_tag="VFD74_02080"
     CDS             6894..>7218
                     /locus_tag="VFD74_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK48376.1"
                     /translation="MIELKGVHKNYGTLEVLRGIDLQITKSEVVVVIGPSGSGKSTML
                     RCMNALEPIQKGTIIIEGIDITNPKTNVNKVRQKIGMVFQSFNLFPHKSAIENVMLAP
                     LKVLKK"
BASE COUNT         1315 a         2399 c         2358 g         1146 t
ORIGIN      
        1 gtctcgacga tgctctcgtt ctcctccttg ctgggagctc cgaccagtgg ggagaagacg
       61 ccggtggcga tcatctcgaa cgagatctcg gcttcgcggt cgaccatgag cgtcggcagg
      121 cgcggaaccc tctctaggac cgcgccgaga gcgtcgcccg cgagcacccg gtcgatgagc
      181 gcgccaccca gaggcgtctg aggcatgcgt gtttctcggg gcgcgtgggg gtaaggcggc
      241 gtcacggggc gcccccgcga acacgggcga agagcggcca gcatggagcg tgcctcaaac
      301 ggcggtcagc tcccgatccg tgccgcgtcg ccatgctatc ctccccttgc gggcaccgtc
      361 cacagggtac gatccggcgc caggccctcg gccatccctc cccgcgtcgc ccggcgcggc
      421 acccatgata gcgaacccga gggccgctgt cgctgtcctt cgccccggcc gtccgccgtt
      481 agaactcgag gcggttccgc aactccttcg tctgcctccg gctcaaaggc aagagggtct
      541 tgcgatcgtc cgcaagccga aggccgaagg tgttcgaagg cagagagatg atctcggaca
      601 cgtgggagag gttgacgatg tagctccgat ggcagcggaa gaactcctta ggatcgagac
      661 gctccgcgag agatcgcaag gagtggctgg ctaggtagct tcctccctgc gccacgaccc
      721 tcgtgtagtc accctccgcc tggaaatagt agatgcggtg aaggtcgagc agcacggtct
      781 tgtcgctacg gttcactgcc agacgttcca gacgcgccgg tctctccttc gcggcccggg
      841 aggtcacgtc caggatggag agcaggcggt cgacggtctg gtcgatgcgc cgctcggtca
      901 cgggctttac caggtagtcg aacgcactca cctcgaaggc cctcacggca aactccgagt
      961 aggcggtgac aaagacgacg taggggttgt cggcgccgcc ggcaagtcgg gtcgccacct
     1021 gcatgccgtc catgtcgggc aggttgatgt cgaggaagac cacgtcgtag ctcaggttct
     1081 cagcgagcgc gagcgcctct tcggcgcctg aggcctcccc gaccaccttg atccgggagt
     1141 ggcggttgag gaggaagcgc aactcggagc gggcgggcgc ctcgtcgtcg acgatgagcg
     1201 cccggtaagt ctccttcatc ggactgactt ggcgaagcgg agtcggcctc gagacccctc
     1261 ttgctgggtc tccacttcgt cagtcaaggc ctggatcacc acgagagaga ggccggcggg
     1321 gtgatcgccg ccgctcagcc cgttcggccc tactgtgccc gcgaccgaga tggaccagcg
     1381 ttcctcggcc agatggaact cgaccgtgat gatgctcggc tcgggagcca ccgctgcgtc
     1441 gcggatgaac agcgagcacg cctcggtcac ggccagcttg aggtcggacg ccgcctcctg
     1501 gtcgagtccg aagcgagtgg cgagcgcggc gatggtcaga cgagagaggg ccacgtattc
     1561 cggccgagcc ggtatcgaga gcacgatagg ggtctcgact gcgtcccgga gatccagaga
     1621 cgctgcggtg ccggtatctt gatgttgcat catgtcaaca atcctatgcg gtttcttcgg
     1681 tccacgccct tgacccgggg gaggccgccg tcgagatggc accgctcgag acgcactgct
     1741 ctgtggcgga gcctaccccc gtgccttcgc ccgctaaaca accggcacgc cgccggacga
     1801 gaggccgcga cccaaggctc ccccggtcgc cgacctctat ttataccttc gattcggttc
     1861 gcaacgcaaa cggtcgaggc cacgcggcgc ctcaggcgga ggtcgacccc atgaccacgc
     1921 gcagatcctc cccgcacgac gcgcaccttc cgtcgcgcac ccacgcctgc tccaccccac
     1981 gatcgccgcg gcggatcgcg gtgcgtccgc aggccggaca gttcgtgttc ctgctcgcgt
     2041 gccccgggac gttgcccaca tacacgaact cgaggccggc actcctgccg aacgcggcgg
     2101 cccgctccag cacggcgatg ggagtcggtt tcaggtagtt gagctcgaaa tccggcagga
     2161 agcgggtcac gtgccacgga gtcttcacac cgaggttata gacgacccag gcggccatgg
     2221 cgttcaggct ctcgtcgtcg tcgttcacgc cggggacgat gttcgagacc acctcgacgt
     2281 gacatccgtg gagtcggacg gcacgaacgg cggcctccat ggccggggcc ggatccttga
     2341 ccttcgtgag ccgcgaccac ccgtccaccc ccggcgcctt cagatcgaac ttgaaggcgt
     2401 ccaggtacgg cgctacgtag tcgagagctt cagatgagag gtatcccgcg gtcacgaacg
     2461 ccgtgtagag cccggcctct cgaaaggcca tggcgacgtc gtgcacgtac tcgatccaca
     2521 cggccgggtc gttgtaggtc caggcgactc ccgccagttt gtgcttgcgc gccatcgcaa
     2581 cggcttcgtc gggcaggagg gtcgggagcc gcgagaactc gccggcaccc gcacccgcgt
     2641 gggatatctg ccagttgggg cacccggcgc agagcacgtt acagcccaag gttccaaggg
     2701 atagcgccct cgtgcccggg tgaaaatgat agagctgctt cttctcgatc tcgtcggaga
     2761 ccaccgtcgc cggacgcccg tagaccagcg actgcatacg cccgtcagtg acgccacgca
     2821 ctcggcacag gcccacaccg ccgttgcgga tgacgcaccg gtgtggacag acggtgcacc
     2881 gagccgaacc tgcggcgaga ggctcccaga ggacggcttc ggggctcacg acgcctccga
     2941 cccgacgtac ggcccggccg agaagcgatg cggcccttct ggagcgactt cgagtcctgc
     3001 ggcagagact cccgtggcag ccgcccgtgc ggcggccttt cctgcggcgg ccgcccctgc
     3061 agcgcttctc cctgcgccca cgagcacgtc gatgccccgc gccacctctg cgagcacggc
     3121 ctcgtggagc gccgggctcg cgcacgcgag caccgacatc tggtgcgaca tatccgatcc
     3181 cagcagcggc cgatcgtcga gactgcggcc cgccgcgtcg gtgacgatac atccggcttc
     3241 ccgcaggatg agggtcgagg ccgccacgtc gtggggggag ttgttgagga ccgcgccctt
     3301 gcccacctca cggaaccgcg cctccaccca cggagccaac tcgatcatgc gcggtccgac
     3361 gtcgacgtag gcgtcgagtt gccccgtcag gatgcgcgtc atggagtacg tagcgctgcc
     3421 gatgtcgaac acggcgccat ccaccgacga gagatcgatg agtcccccca gggcagtcgc
     3481 cagttccagc gccggacggc cccggaagcc tatggtccag aagaggcggg tgaggtcggt
     3541 gttgcttgag atcgacacac accgctccga tccgtcggga gtggtgatgc gcgctccccc
     3601 accacggacg gcgctgaaca ctccgccttc ctttatctcc gccacgaccc cgtagaccac
     3661 atcggacatc accggccggt ctgaagcccg ggccgcggcg acgctcacgc aggcggcttc
     3721 gaaaccggcg gcggcaggtc gcgtgccgtc gatgggatcg actatgagca cgtactcggg
     3781 ggtcgagccg ccgaacctga cgagcccccg gtcctcggag tacacggcaa cgggcaggtc
     3841 tctctgcgtc aggtagctcg cgaggtagtc ctcggccagc tcgtcgatgg cgaaggtggt
     3901 gtcgcctccg gctgcgcgcc ccgtgagacc gcgcgagaag gccgaaccga gggagggcag
     3961 gaccacttcg cggagcccgg aggcaagacc atcgacgagt gcgcgaagcc agccgggaac
     4021 gtcgagggtg gggggttcgg ccgcggctgc gccggcggcc gctcgaccac cgtcgccggt
     4081 gaccgtcacg tcgtgccctc gcgccgccgg tctggcggca agacgggccg gcgcacgcgg
     4141 aaggccgccc ctaggtcggc ggcgaccgac gcggcggcgc ccaggtcgac tcccggcagg
     4201 tcgacgacgc tggccgtgac gtccagcccg gcatcgacgc actccctgat gaagcggagt
     4261 accgcccggt acgccttgga gaacgtcggg cgacagagga gatcgtaggt gaactcgtcg
     4321 tgtgcgttga ggctcaccga ggcggcccgg agaccggagt cggtcaactc gcgcacgacg
     4381 tcgcggccgg ggtgcagtag agccgcgtgg ccgttggtgt cgagacgcgt gggcaggcgg
     4441 cgggtgccca accactcaac ggatcccaag atgacgtcga gctgaagggt gggctctccc
     4501 aacccggtga acacgacctc ctccggagcc ccatccagaa aggccagttc gaacgcgtgc
     4561 acgagatcgg ggacctcagg ctcgtctccc accgccagac tcaggtcgta gccgtagatc
     4621 tcgtcggtga acgaagccag gcagaaggtg cacgaggccg agcagcggct cgtcaggttg
     4681 acgtagaggt tgggccctcc ccagtagacg aagttcggcg ccgcgaagag cgcagcggac
     4741 gacccggaag cgcgcttctc gctgcgcatg cgtgtggaat cgatcatgcc ccggagtata
     4801 gcgtgccgac cacacgtcgg cagcagtagg cgctgaatac cggcctaact cggccgatcg
     4861 atagactgcc gaacgggcct gtggtatgaa tgtaaagttg gcctggggta tcagcgcatg
     4921 gcgaccacgc gcccgatcga acttcgtgag gccgtctggc agccccgcgg aaggccgagg
     4981 gaagtctact agggaggatc acgttgaaga gaagttggct gatagtgctc atgttgcttg
     5041 tcgtcgcgct cgccgttctg ccgctgaccg caggctgcgg cggcgacaag gagacgacca
     5101 ccaccaccgc agcggcagcc ggagacccgg tggccgcggc cgacgcggcg atcgccgcaa
     5161 tcaccaagtc cgacgccatc gagatgccgc cgacactcga ggcgggcaag ttgcaggccg
     5221 gttccgacac gtccttcgcc cccttcgagt tctcagatga gaagggcgga tacatcgggt
     5281 tcgacgtcga cctcgtcacg gccatcgcca agaagatcgg ccttgaagcc gccgtcgtgg
     5341 ccaccgcctg ggacgggatc atcccgaacc tggtcgccga ccggtacgac atcatcatgt
     5401 cggccatgac catcaccgag gaacggcagc agcagatcga cttcaccgac ggctacatcg
     5461 acaccaacct tgccatcacc accaaggtcg acgcgccgat cgctgacgcc gcgggcctcg
     5521 aaggcaagat agtgggcgtg caggtggaca ccaccggcca gttcgaagtg gagaaggtcg
     5581 ccggagtcaa ggagatcaag aagtacgaga acagcatcgc cgccatccag gacctcctgg
     5641 ccggccgcat cgacgcggtc gtgaacgact ccccggtgaa cgcctactac actcgggaca
     5701 agcctgagtt cggcaacacc ggcaaaatcg gttccgacga caagtacgga tatggcatca
     5761 agaaggagag ctcagtactc cgtgacgcca tgaacgccgc tttggccgaa ctgaaggccg
     5821 acggcacgta cgacctgatc tacgcaaagt ggttcggcgg cgacaagtag cgccacggac
     5881 tgatggctag agggactccg gatcggggcc gggacgcaag tcccggcccc gatcgctttc
     5941 tgccgaccac accagttcgt cgacacccgc gaacggctgt ggtatcaata caggccggac
     6001 tccggaacag acccgatcgg gatgcgctat gcgacaagac cgccgcagac cgacgtatga
     6061 agggggaaca cagtggaatc gctgctgaac gggttcgagg cattccagcg ctactacctc
     6121 aactggcaga tcatcaagga aacgaccccg ctgctcctca agaccctgcc gatcgtaggc
     6181 gaactgctgg tcctgagcat gttcttcgcg ttcctcctgg ggctcatcct ctcgctgatg
     6241 aggctctccc gggtccgcgt cttccggtgg ttcgcgggcg cctacgtgga cttcttccgc
     6301 ggcctaccgc tgctgctcaa cttcgtgttc gtctactccg gtctggccat cgtgggagcg
     6361 gcttccgggc tgcggttcct gatcctgcct tcgataccgg cggccgtcct ctcgctctcg
     6421 accacctacg ccgcgtactc ggctgaggtg ttccgggcgg gcatcgaatc catccaccgc
     6481 ggtcagatgg aggccgcgcg ctcgttggga atgacatacc cgcagtcgat gcgctacgtc
     6541 gtgctgccgc aggccatcaa ggtggtgatc ccgccgctga ccaacgagtt catcgccctc
     6601 atcaaggaca cgtcgctcgc atcggtcatc tcggttcagg aactgctctt cacggcaaag
     6661 gagaagatgg gggtggtggc caacccgacc cccctcatgg tggcggcctt cgtgtatgtg
     6721 ctcttcacca tcccgctgat ccggttggcc tcgcgcctcg agaagaagcg ggaggggagt
     6781 tccaaaggag ccaagcccac agatggttcc accggcaaga cgtccgcgcc tgtgctcacc
     6841 gacgtcgcga cccagctccc gaaaggcgga ggtctaggat gagctccgag gtcatgatcg
     6901 aactgaaggg cgtgcacaag aactacggga ccctcgaggt gctgcggggc atcgatctgc
     6961 agatcaccaa gagcgaggtg gtcgtggtga tcgggccgtc cggctcgggg aagtccacga
     7021 tgctccgctg catgaacgcc ctcgagccga tccagaaggg cactatcatc atcgagggca
     7081 tcgacatcac caacccgaag acgaacgtaa acaaggtgcg ccaaaagatc gggatggtct
     7141 tccagagctt caacctcttc ccgcacaagt cggcgatcga gaacgtgatg ctggccccgc
     7201 tcaaagtgct gaagaagt
//
LOCUS       DASRMJ010000071         5634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_41601, whole genome shotgun sequence.
ACCESSION   DASRMJ010000071 DASRMJ010000000
VERSION     DASRMJ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41601"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..497)
                     /locus_tag="VFD74_02085"
     CDS             complement(252..497)
                     /locus_tag="VFD74_02085"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HZK48377.1"
                     /translation="MSKEPLRDLSQVSGIGFTLVSYVLVFTGGGYLLDRLLHTDPWLM
                     VGGVFVGAALGFIYMIRMLTSVSHAEQEPDEDEKHDD"
     gene            complement(547..1800)
                     /locus_tag="VFD74_02090"
     CDS             complement(547..1800)
                     /locus_tag="VFD74_02090"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZK48378.1"
                     /translation="MSGFFADTYGVFAGFALALVVALVVTPVAGRLARRFGAMDKPEA
                     RKIHSVPTPRLGGVAIFFGVMIPSLLLLTNEGAVRGVLIGALLITLVGVVDDVRGIRP
                     LVKLGLQVCVAAILVVYGVRVDTLTIPGLGILVLGWVGIPFTIIWVVALMNIVNFIDG
                     MDGLAAGVCAISSVTFAIIAVSLGRYDMGILAAAVAGSTLGFLRHNFHPARIFMGDSG
                     SLLLGFLLAAITLQGFLKGVATVALVIPLLVLGIPIFDTGFAILRRVKNRRPVYLADR
                     GHLHHRFSNLGYSQRQTVAIIYAWSGLMSAVALAMRFAPKWVTGVLGAVAGIVSLALA
                     YKLEILRWHKVTQLLGGATRPPAGGRPSKEAPDRPPASPPPAERALDRPPASPPAAGP
                     PGRDGIGGERSDSPGERHTEGEKTS"
     gene            complement(1797..2252)
                     /locus_tag="VFD74_02095"
     CDS             complement(1797..2252)
                     /locus_tag="VFD74_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HZK48379.1"
                     /translation="MRPSWDDYFMEIARVVATRSTCTRRHVGAVLVKNRQILTTGYNG
                     AARGLRHCDERGCMRAEAGVPSGERHELCRGLHAEQNAIIQAAFHGVEIAGAEIYCTH
                     QPCVVCAKMLVNAGIVAVHFAGPYPDDLAMEVFTEAGTALREIGGERAS"
     gene            complement(2285..2914)
                     /gene="upp"
                     /locus_tag="VFD74_02100"
     CDS             complement(2285..2914)
                     /gene="upp"
                     /locus_tag="VFD74_02100"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZK48380.1"
                     /translation="MKNVTLVDHPLIAHKLSYLRDETTETKRFRELVAEISTLLCYEA
                     TKDMELEEVTIRTPLEETTAKVIKGKKVAVIPILRAGLGMVEGITNLLPAARVGFVGI
                     YRDHESLEPVQYYCKVPGKMAERLVLVLDPMLATGGSASHAISLMKEKGAADIKLLSI
                     VAAPEGIAAVHRKHPDVPIVVAAIDRGLNDIGYILPGLGDAGDRLFGTR"
     gene            complement(3006..4283)
                     /gene="glyA"
                     /locus_tag="VFD74_02105"
     CDS             complement(3006..4283)
                     /gene="glyA"
                     /locus_tag="VFD74_02105"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZK48381.1"
                     /translation="MDHVPPAAGYSEQTLEQADPVIAALVNRELRRQQENIELIASEN
                     FTPVSVLQAVGSVLTNKYAEGLPGKRYYGGCEVVDQVETLAIERAKALFGAEHANVQP
                     HSGSQANHAAYVSALEHGDVVLAMRLEHGGHLTHGMKINFSGREYIFHHYCLREDTGR
                     IDYDQVREMAREFRPKLIVAGASAYPREIDFAAFRQIADEVGAQLMVDMAHIAGLVAA
                     GVHMSPFGLAEWITTTTHKTLGGPRGGTVFCRSVDAAHLDKSVFPGLQGGPLEHVIAG
                     KAVCFNLAGTEEFRECQQRTVANARALAEELQSGGLRLVSGGTDNHLMLIDFNGSEMT
                     GKAAENLLARVGITANKNSVPGDRRPPTVTSGLRVGTPAMTTRGFGTEEIREVGRIIA
                     ETLTQAPTEERLALLLARGRALTAAHPLYQTLG"
     gene            complement(4350..4793)
                     /gene="rpiB"
                     /locus_tag="VFD74_02110"
     CDS             complement(4350..4793)
                     /gene="rpiB"
                     /locus_tag="VFD74_02110"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZK48382.1"
                     /translation="MRYAVGCDHAGLPLKAPLVAELERQGHEITDFGTDGPESVDYPL
                     FAARVARAVRDGDADRGLLLCGTGLGMAMTANRFRGVRAASVSESFSARMARAHNDAN
                     VLCMGGRVVGPGAAIEILRTWLEQGFEGGRHQRRVDMVMHPDGSD"
     gene            complement(4957..5352)
                     /locus_tag="VFD74_02115"
     CDS             complement(4957..5352)
                     /locus_tag="VFD74_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015124903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZK48383.1"
                     /translation="MTPLTSPPDRLDFDLSVWEVVKQVPPGRVTTYGTVAHLLPVPAG
                     VLETTYAAFGARWVGAAMARCPEDVPWHRVVNARGEVSVREGEGREEQWKRLGLEGVV
                     PGADGRIDLRRFGWHGSGAPGASPQESLF"
     gene            complement(5349..>5634)
                     /locus_tag="VFD74_02120"
     CDS             complement(5349..>5634)
                     /locus_tag="VFD74_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48384.1"
                     /translation="SVVADAAGVDPALLGGCAAAFAGGAAGGGLASTVVDLRPLADGT
                     NALVLREGAVPAGEVLRLVAAAAAAGGADVDGAAAAEAAAESGPKGEGRS"
BASE COUNT          970 a         1971 c         1807 g          886 t
ORIGIN      
        1 gccacgacgt cgaggggcgt gagaaagtga agagccacga gcacgaggcc cgcgatggtg
       61 atgcggccga ggaaggctcc cacagagagg agtgccgccg tgctgctcgc ccgctccgat
      121 agccacaact gcgaacgaga cgaggcgaga tagtacagca cagccatgag gatcccaaca
      181 gctacggatg ccagcgtcag cgccagttcg ttcctccagc ttccttcgtt cgtcgccgtc
      241 agcgaggctg ttcagtcgtc gtgcttctcg tcttcgtcgg gctcttgctc agcatgactc
      301 acggaagtga gcatgcggat catgtagatg aaacccaagg ccgccccgac gaacacgccc
      361 ccaaccatca gccaagggtc cgtgtgcaac aggcgatcga gcagataccc gccgcccgtg
      421 aacacaagca catacgagac gagagtgaac ccgatgccac ttacctgcga gaggtcccgc
      481 aggggttcct tgctcatctc aagagccgcc tcattatagg cagtgacgcc ccgcttggca
      541 agaatgtcac gaagtctttt ccccttccgt gtgccgttca cccggcgaat cggaccgttc
      601 gcccccgatt ccgtctcgac cagggggacc ggcggccggt ggcgatgcgg gtgggcgatc
      661 cagcgcccgc tcggcgggcg gtggcgatgc gggtggacga tccggcgctt ccttggacgg
      721 gcgcccgcct gccggcgggc gggtcgcgcc gccgagcaac tgggtcacct tgtgccaccg
      781 caggatctcg agcttgtacg cgagggccag cgatacgatc ccggcgacgg ctcccagcac
      841 cccggtcacc catttcggcg cgaaccgcat cgccagggcg accgcactca tgagacccga
      901 ccacgcatag atgatggcca ccgtctgccg ctgagagtag ccgaggttcg agaagcggtg
      961 gtgcaggtgg cctcgatcgg cgaggtagac gggccggcgg ttcttgaccc tgcgcaggat
     1021 ggcgaagccc gtgtcgaaga tggggatgcc gagcaccagc agggggatga ccaaggccac
     1081 ggtggccact cccttgagaa agccctgcag cgttatcgcc gccagaagga agcccagaag
     1141 cagcgaaccg gaatccccca tgaatatgcg ggccggatgg aagttgtgcc gcaggaagcc
     1201 gagggtggag ccggccacgg ccgccgccag gatacccatg tcgtagcggc ccagcgagac
     1261 ggcgatgatg gcgaaggtca cggagcttat ggcgcacacc cccgcggcca gaccatccat
     1321 gccgtcgatg aagttgacga tgttcatcaa cgccaccacc cagatgatcg tgaaggggat
     1381 acccacccaa cccagcacga ggatgcccag accggggatc gtgagtgtgt ccacccgaac
     1441 gccgtagacg accaggatgg cggccacgca gacctgcaga cccaacttca ccaggggtcg
     1501 aataccgcgc acgtcgtcga cgacacccac caaggtgatg agcaaggcgc cgatcagcac
     1561 cccacgcacc gcgccctcgt tcgtgagcaa cagcaggctg gggatcatga ccccgaagaa
     1621 gatggccacc cctcccagcc gtggggtggg gacggagtgg atcttccgtg cctcaggctt
     1681 gtccatggcg ccgaatctcc gcgccaacct accggccacg ggagtgacca cgagggcgac
     1741 cacgagggcg agggcgaacc ccgcgaacac gccgtaggta tcggcgaaga atccgctcac
     1801 gacgcccgtt ccccgccgat ctcccgcagc gccgtccccg cttccgtgaa tacctccatg
     1861 gccaggtcgt cagggtaggg accggcgaag tgcactgcga cgatgcccgc gttcacgagc
     1921 atcttggcgc acaccacgca gggctggtga gtgcagtaga tctcggcgcc ggcgatctcc
     1981 accccgtgga aggcggcctg gatgatggcg ttctgctcgg cgtggagccc gcgacagagc
     2041 tcgtggcgct cacccgacgg cacccccgcc tcggcacgca tgcagccccg ctcgtcgcag
     2101 tgacgcaggc cgcgggcggc tccgttgtat ccggtggtga gtatctgcct gttcttgacc
     2161 agcacggcgc ccacgtggcg ccgcgtgcac gtcgaccggg tggccaccac ccgggcgatc
     2221 tccataaagt agtcgtccca gctgggacgc atgctcactc ccctgggccg gggtccgccg
     2281 tccgtcagcg cgtcccgaac agccggtcgc cggcgtcacc caagccgggc aggatgtagc
     2341 cgatgtcgtt caggccgcga tcgatggccg cgacgacgat gggtacgtcg gggtgcttgc
     2401 ggtggacggc ggcgatgccc tcgggcgcgg ccacgatcga cagcaacttg atgtcggcgg
     2461 cacccttctc cttcatgagg gagatcgcat gagacgcgct ccctccggtc gcgagcatgg
     2521 gatcgagcac gagcaccagg cgctccgcca tcttgccggg caccttgcag tagtactgca
     2581 caggctcgag cgactcgtga tcacgataga tgcccacgaa gcccacccgc gccgccggca
     2641 agagattggt gatgccctcg accatgccga ggcccgctct gaggatgggg ataacagcta
     2701 ccttcttccc cttgatgact ttcgcggtgg tctcctcgag aggggtgcgg atggtcacct
     2761 cttcgagttc catgtccttg gtggcctcgt agcacagtag cgtggagatc tcagccacca
     2821 gctcgcggaa gcgtttggtc tcggtggtct cgtcgcgcag gtagctgagc ttgtgcgcga
     2881 tgagcgggtg gtccaccagc gtgacgttct tcatcggatc cttcatcgac tcctgtccct
     2941 cctcgtcgtg catcgagccc tccaacgtcg agccctcaaa cgtcaagccc tccaacgagg
     3001 gcgattcagc cgagcgtctg gtagagcgga tgggcggcgg tcagcgcccg gccccgggcc
     3061 agcaacaagg ccagcctctc ttcagtgggc gcttgcgtaa gggtctcggc gatgatcctg
     3121 cccacctcgc ggatctcctc cgtcccgaaa ccgcgggtgg tcatggcggg agtgcccacg
     3181 cggagacccg acgtgaccgt cggcgggcgg cgatcaccgg gaacgctgtt cttgttggcc
     3241 gtgatgccga cccgagcaag caggttctcg gccgccttcc ctgtcatctc gctgccgttg
     3301 aagtcgatga gcatcagatg gttgtccgtg ccgccggaga ccaatctcag gccgccggac
     3361 tgcagctcct cggccaacgc gcgcgcgttc gcgaccgtgc gctgctggca ctcccggaac
     3421 tcctcggtgc cggccaggtt gaagcagacg gccttgccgg cgatgacgtg ttcgaggggg
     3481 ccgccctgaa gccccgggaa cacggacttg tcgagatgcg ccgcatcgac cgagcgacaa
     3541 aagacggtgc cgcccctcgg tcccccgagc gtcttgtgcg tggtcgtggt gatccactct
     3601 gccagcccga agggcgacat gtgcactcct gccgccacga ggccggcgat atgcgccatg
     3661 tcgaccatga gttgcgcccc gacctcgtcg gctatctgcc tgaaggcggc gaaatcgatc
     3721 tcacgggggt acgcactcgc cccggccacg atcagcttcg gccggaactc gcgcgccatc
     3781 tcgcgcacct ggtcgtagtc gatgcgcccg gtgtcctcgc gcaggcagta gtggtggaag
     3841 atgtactccc ggcccgagaa gttgatcttc atgccgtggg tgaggtgccc gccgtgttcg
     3901 agtcgcatgg cgaggacgac atccccgtgt tcgagcgcgg acacgtaggc ggcgtggttg
     3961 gcctgagacc cggagtgggg ctgcacattc gcgtgctcgg cgccgaacag ggccttggcc
     4021 cgctcgatgg ccagcgtctc gacttggtcc accacctcgc aaccgccgta gtagcgcttg
     4081 cccggcaaac cctcggcgta cttgttggtg agcacgctgc cgaccgcctg cagcaccgag
     4141 accggagtga agttctcaga cgctatgagc tctatgtttt cctgctgccg cctgagctca
     4201 cggttcacca gcgcggcgat caccggatcg gcctgctcca gggtctgctc cgaatacccc
     4261 gccgcggggg ggacgtgatc cacctcagca ttcctcctgt cgtgaaacgg cggacgccgg
     4321 tccgacgccg cttgttcgtc gtatctcgat cagtcgctcc cgtcagggtg catgaccatg
     4381 tccacgcgcc gctgatgtcg accgccttcg aacccctgct ccaaccatgt cctcaggatc
     4441 tcgatcgctg ccccggggcc cacgacgcgg ccgcccatac acagcacgtt ggcgtcattg
     4501 tgggcacggg ccatgcgcgc cgagaacgac tcgctcacgc tcgcggcgcg tacgccacgg
     4561 aagcggttgg cggtcatcgc catgcccagg ccggtgccac acagcagcaa ccccctgtcg
     4621 gcgtcaccgt cgcgcaccgc ccgcgccacg cgggccgcga acaacgggta gtcgacactc
     4681 tccgggccgt ccgtgccgaa atcggtgatc tcgtggccct gacgttctaa ctcggccacc
     4741 agaggagcct tcaaaggtag gccggcatga tcacaaccga cggcgtagcg catgagcctc
     4801 ttcccctttc gatgccgctg gaaagcatag cagaaaggac cctaccggcg ccgcgttgcg
     4861 gaggcctcgc ccgcagccaa ctaccgcgac cgcgggagcc gcagaccacc ccgagagcgg
     4921 acattctggc tgctgccgcg gccgcccgcc ccccgatcag aacagcgact cctgggggga
     4981 cgcccccggt gccccgctcc catgccatcc gaaacggcgc agatcgatac gcccgtcggc
     5041 tccgggcacc acgccctcga gcccgaggcg cttccactgc tcctcgcggc cctccccctc
     5101 acgcacactc acctctccac gggcgttcac cacccggtgc cacggaacat cttcagggca
     5161 acgtgccatc gcggccccca cccagcgcgc cccgaaggca gcatacgtgg tctcgagaac
     5221 cccggccggc acgggcaaca ggtgggctac cgtcccgtag gtggtcaccc ttccaggagg
     5281 cacctgcttc acgacctccc atacgctcag gtcgaagtcg aggcggtcgg ggggcgaggt
     5341 gaggggcgtc acgaccgacc ctcgcccttg gggccgctct ccgcggcggc ctcggccgct
     5401 gctgcgccgt cgacgtcggc cccccccgct gcagccgccg ccgccaccaa tcgtaggacc
     5461 tcacctgccg gaacggcgcc ttccctcaag acgagggcgt tcgtgccgtc tgccaggggt
     5521 cgaagatcga cgacggtgga cgcaaggccg ccgccggctg cgccgccggc gaacgccgcg
     5581 gcgcagccgc cgaggagagc gggatcgacc ccggctgcgt cggcgacgac ggac
//
LOCUS       DASRMJ010000072         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_477339, whole genome shotgun sequence.
ACCESSION   DASRMJ010000072 DASRMJ010000000
VERSION     DASRMJ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477339"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFD74_02125"
     CDS             <1..687
                     /locus_tag="VFD74_02125"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZK48385.1"
                     /translation="LQNGLKNLQRISEVLDEAIVVGGVTAHSAMPLGPNVIKYNGGLG
                     GISIGRYDGMEDPGLQRVIDAFTAAGIPTNRIAGDVRIPIWRKLLANVSCNAVAALTG
                     FTGRQLVEFQPTNDLIRALAEETAEVARAQGFDFAELEFAGDFAIKALSGVGDNKISM
                     LQDVEAGRRTEIDTLNLAVVERGEVYGVDTPLNWMIGILIQALEQRMRFEREAGVTAP
                     ATDKKRSTQE"
     gene            684..2255
                     /locus_tag="VFD74_02130"
     CDS             684..2255
                     /locus_tag="VFD74_02130"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZK48386.1"
                     /translation="MNIADALDRNAIFFPDHEALVDSARRWTYGQFHRDAGRLAHALI
                     DLGVQKGDRVCLFTGNSAEFAIAFYGILKVGAIVVSISSMSRKREVEYMTNNCEAEVM
                     IAGWDYADELPDRADIPRVRQVVVVAPTGSADGSDRSGTGDGSDVPVLPTGVDHSFWP
                     LMDGREDPFPTVYTDREDGAEIIYTSGTTGRPKGVVLTHDNVVSNTYSTMYEAGVSRD
                     DRAVCFLPMYHSFAQNFIFNSTVRAGATLIILPKFEAENTLRVMAEERVTRWHAVPTI
                     YILVLGMPEAEEAFRSVTFCFSAASTMPQEVARQWYDRFGLKINEGYGLTESTPSAVY
                     NHTVRHKPGTVGTPIMNVEVQIWDADCNVLPVGSVGEIVLKGPNVMKGYYNDPVATAE
                     SLVDGWLRTGDVGVLDEEGYLSIVDRIKDMVNSAGLKIWPREVEEVLYTHEAIKECAV
                     IGVADPLFGENVKACIVLRSGATLCADDVVVFCKERLSSYKAPKIVEFMESLPKSATG
                     KILKTELRKMSEGSE"
     gene            2252..>2768
                     /locus_tag="VFD74_02135"
     CDS             2252..>2768
                     /locus_tag="VFD74_02135"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HZK48387.1"
                     /translation="MIGRDGEVASAARASARGSAHGSAGAGAAALERLRTVGVDPLSE
                     LAAVKERTGLMAVGYFDSYLPEELIMAHGLVPYRVTVSGRDDVRSSEFIQGYACPAAR
                     DLLEQALRGDLSLLDGVLFTRYCDSLRGVFAVWDSEKLSPFVDFVRYPTVVDTSTAVG
                     YLARELQEVSVR"
BASE COUNT          502 a          846 c          914 g          506 t
ORIGIN      
        1 cttcagaacg ggctgaagaa cctgcagcgt atctcggagg tgctcgacga agctatcgtg
       61 gtgggaggtg tcacggcgca cagcgcgatg ccccttgggc ccaacgtcat caagtacaac
      121 ggtggcctgg gcggtatcag tatcggccgt tacgacggga tggaggatcc cggtctgcag
      181 cgtgtgatcg acgccttcac ggcggctgga atccccacca accgcatcgc cggagacgtc
      241 cgcatcccca tctggcgcaa gctgctggcc aacgtatcgt gtaacgcggt cgcggcgctg
      301 acggggttca caggacgtca gctggtggag ttccagccca ccaacgacct catccgagcg
      361 ctggcggaag agactgccga agtcgctcgc gcccaaggat tcgatttcgc cgagctggag
      421 ttcgcggggg atttcgccat caaggcgctg agcggggtgg gcgacaacaa gatctccatg
      481 cttcaggatg tggaggccgg gcggcgcact gagatcgaca cgctgaacct ggcggtggtg
      541 gaacggggcg aggtgtatgg cgtcgacact ccactcaact ggatgattgg tatcctgata
      601 caggcgctgg agcagcgcat gcgtttcgag cgcgaggccg gcgtgaccgc cccggcaacc
      661 gacaagaaga ggagcacaca ggagtgaaca tagccgacgc cctcgaccgt aacgctatct
      721 tcttccctga ccacgaggcg cttgtcgata gcgcgcgtcg ctggacgtat ggacagttcc
      781 accgggacgc gggccgcttg gctcacgccc tcatcgacct cggtgtccag aagggcgacc
      841 gcgtctgtct gttcacgggc aacagcgctg agttcgccat cgccttctat gggatcctga
      901 aagtgggggc gatcgtcgtc tccatctcgt ccatgtcccg gaagcgcgag gtggagtaca
      961 tgaccaataa ctgcgaagcc gaggtcatga tcgccggctg ggattatgcg gacgagttgc
     1021 ccgatcgggc cgacataccg cgcgtgcgac aggtggtcgt cgtggcgccg accggctccg
     1081 ccgacggctc cgaccgtagc ggcaccggcg acggctcgga cgtcccggtc ttgcccaccg
     1141 gggtcgatca cagcttctgg ccactcatgg atgggcgcga ggatcctttt cccaccgtct
     1201 acaccgaccg tgaagacggc gctgagatca tctacacctc gggcaccacc ggcaggccca
     1261 aaggggtcgt gctcacgcac gacaacgtgg tctccaacac gtattcgacc atgtacgagg
     1321 ccggcgtgag tcgagacgat agagccgtgt gtttcctccc gatgtaccat tccttcgctc
     1381 agaacttcat cttcaacagc actgtccgtg ccggagccac tctcatcatc cttccgaagt
     1441 tcgaggcgga gaacacgctg cgggtcatgg ccgaggagag ggtcacacgt tggcacgcgg
     1501 tccccaccat ctacatcttg gtgctgggca tgcccgaagc ggaggaggcc ttccggagcg
     1561 tgaccttctg cttctcggct gcatcgacca tgcctcagga agtggcgcgc cagtggtacg
     1621 accgattcgg cctcaagatc aacgaaggct acgggctcac cgagtccact ccgtccgccg
     1681 tgtacaacca cacggtgcga cacaagcccg ggacagtcgg cactcctatc atgaacgttg
     1741 aggtgcagat ctgggatgcc gactgcaacg ttctccctgt gggttccgtg ggtgagatcg
     1801 tgctcaaggg tcccaacgtc atgaagggtt actacaacga cccggtggcg acggcagagt
     1861 cgctggtcga cggctggctg aggacaggcg acgtcggcgt cctcgacgaa gagggctacc
     1921 tctccatcgt cgaccgcata aaggacatgg tcaactcggc cggcctgaag atctggccgc
     1981 gtgaggtgga ggaagtgctc tacacgcacg aggcgatcaa ggagtgcgcc gtgatcggcg
     2041 tcgccgaccc cctctttggc gagaacgtga aggcctgtat cgtactgcgc tcgggtgcga
     2101 cgctgtgcgc ggacgacgtg gtggtcttct gcaaggagcg tctctccagc tacaaggcgc
     2161 ccaagatcgt ggagttcatg gagtctctgc ccaagagcgc taccggcaag atcctcaaga
     2221 cggaactgcg gaagatgtcc gagggatcgg agtgatcggg agggatggcg aggtggcgtc
     2281 tgccgcgcgt gcgagtgcca gaggctccgc ccacggctcg gccggtgccg gcgctgcggc
     2341 gctggaacgc ctgcggacgg tgggcgtcga tccgctctcc gagctcgccg ccgtcaagga
     2401 gcgcaccggc ctcatggctg tgggctactt cgatagctac ctgccggaag agctgatcat
     2461 ggcccacggg ctcgttccct accgggtcac ggtgagcggc cgggacgacg tgcgctccag
     2521 cgagttcata cagggctacg cctgtcccgc ggcgcgtgac cttctggagc aggctttgcg
     2581 cggtgatctc tcactgttgg atggcgtgtt gttcacgcgc tactgcgact ctctccgcgg
     2641 cgtcttcgcg gtgtgggatt cggagaagct ctcgcccttc gtcgacttcg tgcggtatcc
     2701 cacggtggtg gacacatcga cggctgtcgg ctacctggcg cgggaactgc aggaggtatc
     2761 ggttcgga
//
LOCUS       DASRMJ010000073         6048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_294480, whole genome shotgun sequence.
ACCESSION   DASRMJ010000073 DASRMJ010000000
VERSION     DASRMJ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294480"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VFD74_02140"
     CDS             <1..817
                     /locus_tag="VFD74_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZK48388.1"
                     /translation="SPAAAPAAAPAAAAAPAGAPPVRAALVTGASSGLGRAIAFELAA
                     NGYRVMLAGRDRSRTEQAADSVAEAGGTAHVWLGDLSGPAACQDVVARAVEVMGRLDL
                     LVNCAGLIYRATAEQTTDEEWRHTMSVNVDAVFYTSRAAIPVMRAQGRGAIVNVASDW
                     GLVGGELAVAYCASKGAVVLMTKAMALDHAREGIRVNAVCPGDCDTPMLDTEAAQRGV
                     DPLESRGEAGEAVPVGRIGWPEEVAKLVAYLASDAAAFITGAAIPIDGGNTAG"
     gene            complement(850..1806)
                     /locus_tag="VFD74_02145"
     CDS             complement(850..1806)
                     /locus_tag="VFD74_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZK48389.1"
                     /translation="METQGRKTRAALISVASNTTLTLLKLLVGIAIGSVGVLSEAIHS
                     GIDLIASVIALFAVRRSGRPADEEHPFGHGKVENISGVAEALLIFVAAGWIIFEAVGK
                     LRHPEPLETIGWGVGIMLLSAVANLWVSRMLFRVGKETASVALQADGWHLRTDVFTSA
                     GVMGGLALIVVGQRVFPALDLDWIDPMAGIVVALLIVRAAYRLTTDATRDLMDSSLAD
                     EEIWIRAHLADLKPSIRGYHKLRTRRAGNTRFVDVHVLLDSSTHLDQAHEISQKIEDA
                     VRDRFPGTSLTVHMEPCDADCAPICVQGCLLDPQERGSLTGR"
     gene            2050..2997
                     /locus_tag="VFD74_02150"
     CDS             2050..2997
                     /locus_tag="VFD74_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HZK48390.1"
                     /translation="MIVTTKELFAAAYRKYAIGAYNINNLEQAMGLFRGNLDSQAPFI
                     IQISKGARSYTDKRMLEGIIRAADEVFPEAIFAVHLDHGDHAACIDCIESGFYSSVMI
                     DASSQPLAENIRITREIVEAAHAKGIVVEAELGQLGGVEEHVQVEEKNAHLTDPADAK
                     AFVEGSGCDSLAVAIGTSHGAYKFSGAQALRLDVLAEIQKRLPGFPLVMHGSSSVPQE
                     EVQRINDAGGAMKGAKGVNEDEFTKAVPLGVTKVNIDTDGRLVWTRVHREFFLDRPEK
                     FDLRDPGTIFMAEYAKFIAHKNVKLGSAGRLEEVRKVVS"
     gene            complement(3053..4369)
                     /locus_tag="VFD74_02155"
     CDS             complement(3053..4369)
                     /locus_tag="VFD74_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK48391.1"
                     /translation="MAQRDRAHDGTHIPEVHLNLNVRGLGMSATLAINERSAQLRREG
                     RRVFKLGLGQSPFPVPRCVVEELRNHAHEKDYLPVRGQRELRDAVADYHRDTKSLECT
                     GPDVLVGPGSKELMFILQLVYYGDLVIPTPSWVSYAPQARIIGRPIRWVPARAVNAWR
                     IMPEDLDLICRADPGRPRILVLNYPSNPTGTTYSAEELEALAEVARRYRVVLLSDEIY
                     GELNHQGAHVSIARYYPEGTIISGGLSKWCGAGGWRLGTFAFPRQLRWLLEAMAVVAS
                     ETFTSTSAPIQFAAVRAYQGGTEIQEYLWQSRRILSALGRHCHRRLVESGLGVPEPQG
                     GFYLFPDFGPLRDRLRERGIDTGADLCNRLLADTGVALLPGADFGRPLEELTARLAYV
                     DFDGARALAGAEHVESRLELGTDFLRAYCGNVLDALDALQAWLRES"
     gene            complement(4393..4866)
                     /gene="arfB"
                     /locus_tag="VFD74_02160"
     CDS             complement(4393..4866)
                     /gene="arfB"
                     /locus_tag="VFD74_02160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HZK48392.1"
                     /translation="MVLPSSEAGLDRRPSTDDIVVSDDLVIPAREIRFTFSRSGGPGG
                     QNVNKVNTRVTLLFEPETSSALAPEQMALIKERLASRIGSDGVLRVVSQQSRSQSANR
                     QAARERLARILADALYEQPERRDTHPPRSVTRKRIEDKRVRGRLKQSRRRDWPDE"
     gene            5048..5386
                     /locus_tag="VFD74_02165"
     CDS             5048..5386
                     /locus_tag="VFD74_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZK48393.1"
                     /translation="MKKIEAIIKPFKLDEVKDALQAVGVHGLTVTEVKGFGRQRGHTE
                     VFRGVEYQVDFVPKVKVEVVVEAAAAPAVVDAIAQAAQTGKIGDGKIFVSPMEEVVRI
                     RTGERGHDAL"
     gene            5399..>6048
                     /locus_tag="VFD74_02170"
     CDS             5399..>6048
                     /locus_tag="VFD74_02170"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="catalyzes the uridylylation or deuridylylation of
                     the PII nitrogen regulatory protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein"
                     /protein_id="HZK48394.1"
                     /translation="MIASMTQDSPGHLRDALAELRYRHLAGAGGRELCRRRADVVDLA
                     LISLFRQAEHESPPGLYRQAGLVVVALGGYGRRELCPHSDIDLMLLYRPEERARVESV
                     AETLFYPLWDAGLELGHGARTVDECLEVAEDSLELLTSFFQARLLVGDQELFRELMKR
                     LRASVNKDGGAGFVEAVRAARATRHGTFGDAHVLLEPHLKEGRGGLRDVHEALWLA"
BASE COUNT          994 a         1930 c         2109 g         1015 t
ORIGIN      
        1 ctcccccgca gctgcacccg ccgctgcacc cgcggccgca gccgcacccg cgggcgcccc
       61 tcccgtccgc gccgcgctgg tcaccggagc gagttccggc cttggcaggg ccattgcgtt
      121 cgagctggcc gccaacgggt atcgggtcat gctcgcaggc cgtgaccgct cgcgcaccga
      181 gcaagcggcg gattccgtcg cggaggccgg gggcaccgcg cacgtgtggc tgggcgacct
      241 gtccggcccc gcggcgtgcc aagatgtcgt ggcgcgcgcg gtggaagtca tggggcggct
      301 cgatctcctg gtgaactgcg ccggcctgat ctatcgtgcc accgcggagc agaccaccga
      361 cgaagagtgg cgccacacga tgtccgtgaa cgtcgacgcc gtcttctaca ccagccgggc
      421 cgccatcccg gtcatgcgtg ctcagggtcg gggagcgatc gtgaacgtgg cctctgattg
      481 ggggctggtc ggcggggagt tggctgtggc ctactgcgcc agcaagggtg ccgtcgtgct
      541 gatgaccaag gccatggctc tcgatcacgc ccgcgaaggc atacgcgtga acgccgtctg
      601 cccgggcgac tgcgacacgc ccatgctcga caccgaggcc gcgcagcggg gcgtggatcc
      661 cttggagtcg cggggggagg ccggcgaggc cgtcccggta ggtcggatcg gctggccgga
      721 agaggtggcg aagctcgtgg cctaccttgc ttccgacgcc gcggccttca tcacgggagc
      781 ggccatcccc atcgatgggg gcaacacggc gggctgaccc gtgggcgtcg gtcctgagat
      841 gggcctgccc tatcgtccgg taagagaacc tcgctcctgc ggatcgagca ggcagccctg
      901 aacgcatatg ggggcgcagt cggcgtcgca gggttccatg tgcaccgtga gactggtgcc
      961 ggggaaccgg tcgcggaccg cgtcctcgat cttctgcgag atctcgtgcg cttggtcaag
     1021 gtgggtgctg ctgtccagca agacgtgcac gtcgacgaag cgcgtgttgc cggcccggcg
     1081 tgtgcgcagc ttgtggtatc cacggatgct gggcttgaga tcggcgaggt gggcccgtat
     1141 ccagatctcc tcgtcggcga ggctcgagtc catcagatcg cgggtcgcat ccgttgtgag
     1201 acggtacgcc gcccgcacga tcagcagggc gaccactatg ccggccatcg gatcgatcca
     1261 gtccaagtcc agagcgggga agacgcgctg gcccacgacg atcagcgcca ggccgcccat
     1321 gacgccggcc gaggtgaaca cgtcggtgcg gagatgccag ccgtctgcct gcagagcgac
     1381 ggaggcggtc tccttgccca ctctgaacag catgcgcgag acccagaggt tggcgaccgc
     1441 ggagagcagc atgatgccca cgccccagcc gatggtctcg agtggttccg ggtggcgcag
     1501 cttgccgacg gcttcgaaga tgatccagcc ggcggccacg aatatgagaa gggcttcggc
     1561 cacccccgag atgttctcca ccttcccgtg gccgaagggg tgctcttcgt cggccgggcg
     1621 gccggaccgc cgcaccgcga agagagcgat gaccgacgcg atgaggtcga tgcccgagtg
     1681 gatagcctcg gagagcacgc ccacagaccc gatggcgata cccacgagca acttgagcag
     1741 cgtcagcgtc gtgttcgagg ccaccgagat caacgctgcc cgcgtcttgc gaccctgcgt
     1801 ttccacgctc tgcacctttc gacaagaagc cccatgggag gcagtcccac gggggcccaa
     1861 gggttccgtt gccccgaagg gcccggcgcg cgctgcggat ggtccgggcg ccgcctgttc
     1921 tgtggcttcg agcaagtata gccgtcgtgt tccggccggc caagcgtcgc gccggctcgg
     1981 tcgggggttg accaccccga gcccacctgc tatgaatcga ggcgacccct acatcaagga
     2041 ggacaggttg tgatcgtcac caccaaggag ctcttcgcgg ctgcttacag aaagtacgcc
     2101 atcggagcgt acaacatcaa caacctggaa caggccatgg gactcttccg tggcaatctg
     2161 gattctcagg cacccttcat catccagatc tcgaagggcg cccgatcgta caccgacaag
     2221 cgcatgctcg aaggcatcat ccgggcggcg gacgaggtgt tccccgaggc catcttcgcc
     2281 gtgcatctgg atcacggtga ccatgcagcg tgcatcgact gcatcgaaag cggcttctac
     2341 tcgagcgtca tgatcgatgc ctcgagccag cctctcgccg agaacatccg catcaccagg
     2401 gagatcgtgg aggccgccca cgccaagggc atcgtggtcg aggcggaact cggccagctg
     2461 ggtggagtcg aggaacacgt gcaggtggag gagaagaacg cccacctcac cgatcccgcg
     2521 gatgccaagg ccttcgtcga aggctcggga tgcgacagcc ttgccgttgc catcggcacg
     2581 agccatgggg cctacaagtt ctcaggcgct caggctctac gactcgacgt cctggccgag
     2641 atccagaagc gccttcccgg gttcccgctg gtcatgcatg gctcgtcgtc ggtgcctcag
     2701 gaggaagtgc agcgcatcaa cgacgccggc ggagccatga agggcgccaa aggtgtgaac
     2761 gaggatgagt tcaccaaagc ggtgcctctg ggcgtgacca aggtgaacat cgacaccgac
     2821 gggcgtctgg tatggacccg cgtgcatcgg gagttcttcc tggataggcc cgagaagttc
     2881 gatctgcgcg accccgggac gattttcatg gcggagtacg ccaagttcat cgcccacaag
     2941 aacgtgaaac tggggtcggc cggtcgcctc gaagaggtgc ggaaggtggt ctcctagttc
     3001 ggccggcgct ggccggcgct ggcggcgctc ccggccgggt ccgcggcctg gctcaactct
     3061 ccctgagcca ggcctgcaac gcgtcgagcg catccagcac gttaccgcag taggcccgaa
     3121 gaaagtccgt gccgagttcg agtcggctct ccacgtgttc ggcgcccgcg agggctcggg
     3181 cgccgtcgaa gtccacgtag gcgaggcggg cggtgagttc ctccaagggt ctgccgaagt
     3241 ccgcgcccgg gagcagagcc actcccgtgt ctgcgaggag tctgttgcag aggtcggcac
     3301 cggtgtcgat gcctctctcg cgaaggcggt cccggagggg tccgaagtcg gggaagaggt
     3361 agaaaccccc ttgcggctcg ggcacaccta acccgctctc cacgagtctg cggtgacagt
     3421 ggcggcccag tgcgctcaga atgcgccggg attgccatag gtactcttgg atctcggtgc
     3481 cgccctgata cgcgcgcacc gcggcgaact ggatgggggc gctggtgctg gtgaacgtct
     3541 cgctcgccac cactgccatg gcttcgagta gccaccgaag ctggcggggg aacgcgaagg
     3601 tgccgagccg ccagccgccc gccccgcacc acttgctgag tccgccgctg atgatggtgc
     3661 cttcggggta gtagcgcgcg atggacacgt gagcaccctg gtggttgagt tccccgtaga
     3721 tctcgtctga gagcaacacc acgcggtatc gccgggccac ctcggccagg gcctccaact
     3781 cttcggccga gtacgtggtt ccggtgggat tcgaggggta gttgagcacg aggatgcgtg
     3841 ggcgacccgg gtccgcacga cagatgaggt cgagatcctc gggcatgatc cgccaagcgt
     3901 tgacggcacg cgcgggcacc cagcggatcg gtcggccgat gatgcgtgcc tggggcgcgt
     3961 acgagaccca gctcggggtg gggatcacca agtccccgta gtacacgagt tgaaggatga
     4021 acatgagttc cttggagccg gggcccacga gaacgtccgg acctgtgcac tcgagggact
     4081 tcgtgtcgcg atggtagtcg gcgaccgcgt cgcgaagttc tctctgcccg cgcacgggca
     4141 gataatcctt ctcgtgtgcg tggttgcgga gttcctcgac cacacagcgg ggcacgggga
     4201 agggcgactg gcccagcccc agcttgaaga ctcgccgacc ctctcgccgc agctgcgcac
     4261 tccgttcgtt gatggcgagg gtggccgaca tgcccaaccc gcggacgttg aggttgaggt
     4321 ggacctcagg gatgtgcgtt ccgtcgtgag cacggtcgcg ctgtgccatg atgggctcct
     4381 cgtctgggga cgtcattcgt ccggccaatc acgtcggcgt gactgcttga gcctaccgcg
     4441 cacccgcttg tcctcgatgc gctttcgggt gacactccgg ggcgggtggg tgtcgcgcct
     4501 ctcgggttgt tcgtagaggg catcggccag tatgcgggcc agtctctcac gcgccgcctg
     4561 acggttcgcg ctctgcgatc gagattgctg cgagaccacc cgcaacaccc catcggagcc
     4621 gattcgcgag gccaggcgtt ctttgatcag agccatctgc tcgggcgcga gcgccgagga
     4681 tgtctccggt tcgaagagaa gggtcacccg ggtgttcacc ttgttcacgt tctgtccccc
     4741 gggacctccg ctccgcgaga atgtgaagcg gatctctcgc gcggggatga ccaggtcgtc
     4801 tgagacgacg atgtcgtcgg tggaaggtcg tcggtcgagc ccggcttcgg aggatggcag
     4861 caccatgctc caaccatagc tgatgcggtc gcggggagcc agttggacag ccaacggcgg
     4921 ccggcctttg tccgctggtc cattggtgcg cgccgtggtc gcgggtatag tctggctcac
     4981 gcctgacagg tttccggcgc ggggtcgagt gtgacgcggg cgcggcgggt gccgcgagga
     5041 ggacgggatg aagaagatcg aggccatcat caagccgttc aagctcgatg aggtgaagga
     5101 cgccctccaa gcggtgggcg tccacgggct caccgtcacc gaggtgaagg ggttcggacg
     5161 gcagcggggg cacaccgagg tcttccgcgg cgtggagtat caggtggatt tcgtgccgaa
     5221 ggtcaaggtg gaggtggtgg tggaagcggc cgccgctccc gcggtggtgg acgcgatcgc
     5281 ccaggcggcg cagaccggca agatcggcga tggcaagatc ttcgtctcgc ccatggagga
     5341 agtggttcgc atacgcaccg gcgagagggg gcacgacgct ctctagcggc ggcccgtcgt
     5401 gatcgcatcc atgacccagg actcacccgg ccaccttcga gacgctctgg ccgaactgcg
     5461 gtatcgacat cttgccggag ctggaggtcg ggagctgtgc aggcggcgcg ccgacgtggt
     5521 cgacctggcc ctcatctcgc tcttccgcca ggctgagcac gagtcgcctc ccggcctcta
     5581 tcgccaggcc gggctggtcg tggtggcgct cggcggctac ggtcgcaggg agctctgccc
     5641 gcactcggac atcgatctca tgctgctcta ccggccggag gagcgtgccc gagtggagtc
     5701 cgtggccgag accctcttct atcccctgtg ggacgctgga ctcgaactgg gacacggcgc
     5761 gcgcacggtg gacgaatgtc tcgaagtggc cgaggatagc ctcgagctgc tcacctcctt
     5821 cttccaggcg cgcctgcttg taggcgacca ggagctcttc cgcgagctca tgaagcggct
     5881 ccgagcctcg gtgaacaagg atggcggcgc cggcttcgtc gaggccgtcc gcgccgcccg
     5941 cgcaacccgg cacggcacct tcggagacgc gcacgtactg ctcgaaccac acttgaagga
     6001 ggggaggggt gggctgcgcg acgtacacga ggcgttgtgg ctcgcctt
//
LOCUS       DASRMJ010000074         9111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_41778, whole genome shotgun sequence.
ACCESSION   DASRMJ010000074 DASRMJ010000000
VERSION     DASRMJ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41778"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..2190)
                     /locus_tag="VFD74_02175"
     CDS             complement(58..2190)
                     /locus_tag="VFD74_02175"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZK48395.1"
                     /translation="MWKRVQAATEYYAPVPGGRRFPLFRTSILLPALTVMFGMQVLRV
                     LLPLLVYVLRDRFGLQALHLGVLAIVLFAGSFLVGPLLKALGPRRLAMFTAIGLGLAR
                     LLIQAWVWDPAVDLILAVVGTLLFLLYLPAGLVLAGWRTAGGGIGSGSTAAYALGILA
                     GLGLDTALNGVLQTYDLAWRAGWLVLLVVLVLALLQTHLAYHDRLKVGAGSRAASARG
                     RLLAGPWIAFGPILALEMLLFQNVARLGTLTGLSVPGAAFLAVVAHALGLVAAGWLYV
                     MQGTPPVTRGAGGSTESVPVAVVARALAMAAGVLLVLSVIPAAPEGMDAAVLFVTGQV
                     ATAYLLMVVVGSVGAGRVSTVPPSGSLGGYGRLSPFHGVGMLIMVIILFLAYSGYDIA
                     LPFSADVLPPFAAILVGVCGVWAIVGRRRLDRANGADSLPVGLPSRIRRLMPALIVAV
                     IAVAPLIQWAVFEPAQAREGDGLPVRVMTLNLHNGFATDGRLDLEALACVIEAENPDI
                     VALQEVSRGWVINGTVDSLGWLQARLGMPAVYGPTAGPLWGNALLTRLPIEESELASL
                     PTPELLLQRGYIKAKIDVGGGRSLDVVATHYHHTEDGSQVRVLESESLLQAWGEAKDL
                     VVMGDMNAEPGSPEIETLRDAGLVEILQAAGIAPGYTYSSDEPFQRIDYIWVSPDLAG
                     PSGSSLTDVRVTTGTASDHFGIAATLGR"
     gene            complement(2303..4735)
                     /locus_tag="VFD74_02180"
     CDS             complement(2303..4735)
                     /locus_tag="VFD74_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK48396.1"
                     /translation="MVAPSVVTWVLTSVVASVVSSTLTLSSLRFSYIVKRWLIGTVFF
                     LAVLAGGMAVRATGYGEAPRAPEQGAGTGPVVSVVTTLPAAGAATSSTEPRAVAAPDP
                     GSDDALLLERPAGERIARVDHDAFPGGDDPSDPDGTAYYVASGGDDSGPGSSELPFAR
                     IERALSVAADGDRVIVHAGVYPSGGLTVTQSRFSLAAAGDGVVVVQADGPDAGYGLAV
                     SAPGQHDVLVRGLTIAGFDSVGVHYGNPVAVERLVFEDLVVEGAPAGMSSDYLPGDTI
                     VDGLLVRRVSLLGIGDVGLTFGVAGGRNVHIVGLHVSMADAAAATARDAVGAGVGAGT
                     GVGTTTIAASAGGVVIGAGDNVLIENSIVEGGRGDGIDLGATRSAVVSTIVRRVGRSG
                     ITLRAGGDVVDSLVFDAGGDAQLATGSGSYRVVNCTFAYHDRAGGGGAAGGTAGGNAA
                     TSGGAGGGPYAAAFGVGATAGQDMDVTLLNNLFYREPGPLDFPAGARVRAENNVFWGF
                     PDRIVVSGARSFGPDDLASSLGVGNLVAEPSLVDPERGDFHPGVGSPLFSAGSHSEEA
                     PQSDLLGLPRVESVTAGAFQTTHSGTPHSAVPTVSTTSTIVSTVVSTAAPTLPVFNDV
                     PSSHAYALAVAGLASRGVVAGYGGGGGSLEFNPDAPVLRAELAKMVVRTLGLTVFESM
                     PQPFSDLGPDDPVDLYPHEYVAAAADAGAFVGIGGGLFAPYAEISRAQAITVVVRAAA
                     NAVPTALSAAPSDFRSTLGSFDLTHDESIRTAEANSLLDGLVGFGPAWDPRAPATRGE
                     AASILWRLFERR"
     gene            4993..5087
                     /locus_tag="VFD74_02185"
     tRNA            4993..5087
                     /locus_tag="VFD74_02185"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5028..5030,aa:Ser,seq:gct)
     gene            5091..5164
                     /locus_tag="VFD74_02190"
     tRNA            5091..5164
                     /locus_tag="VFD74_02190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5125..5127,aa:Arg,seq:acg)
     gene            5339..5929
                     /locus_tag="VFD74_02195"
     CDS             5339..5929
                     /locus_tag="VFD74_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005550405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HZK48397.1"
                     /translation="MNRMNPKVDEYLSKATKWQEEFAQLRTILLDCELTEEVKWGWPC
                     YMFQMSNVVLINGFKEYCALLFFKGALLSDVNGILNMIGENTQAGRQIRFTNVGEIVA
                     LTPILQAYIYEAIEVERAGLHVTLKKNIDIVFPEEFQRRLDGAPALKTAFDALTPGRQ
                     RAYNMYFSQPKQSKTRESRVEKCVERILDGKGLNDQ"
     gene            complement(5980..8388)
                     /locus_tag="VFD74_02200"
     CDS             complement(5980..8388)
                     /locus_tag="VFD74_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HZK48398.1"
                     /translation="MIVAVYLLTFIILDFASQQFEELRGVVAWYPPAGLIYALLLVFG
                     VRLVPAVTIVYFFGSLFIYRMPQSPYALLLWAVIVSSIYGAAAALLRHRIRFDWHLRT
                     SRDVTRLVVTAALVSALLAVLSVSSSTLSSSLPRSEFFRALFLWWIGEMVGVLTVTPF
                     LLVHVMPWLERFAEGRRRVTLSGRRLRLRLTPSVVGQAFSLVLMFYWVFGVHVLEEFR
                     PTYLVTLPLIWIALDHGFKGVTTGIVASNFGMTLAMWLFRLDLGLLGQLQLLMIVNCM
                     VGLLVGAVVTERRHAETTLFEREERFRTLIEKAVDLTIELAPDGICRYVSPSVERMLG
                     FTPEEIVGESLVGFIHADDRPAMLDSIAARPQTLPRADRPTELRVRHRNGSFRTIEVF
                     VNALVDDPALDRIVINARDVTERVEAEEEREGLEAQLQQAQKMEAVGRLAGGVAHDFN
                     NMLGVILGHAEMALEVVGPGQPLHADLTEIRRAAERSADLTRQLLAFARRQTVVPRVL
                     NLNETVADMLSMLERLIGEDIDLNWQPGAELWPVKVDASQIDQILANLCVNARDAGAR
                     RLTIETGRSTFDEAYCGHHPGSAPGEYARMTVADDGCGMDEETLAHAFEPFFTTKGVG
                     TGTGLGLATVYGIVKQNGGFIDVRSGRGEGTTITVYLPRTTETDQPRTEASTVSTARG
                     HETILLVEDEPALLEMTAIMLEIEGYTVLAAGGPGEAIRLTREYEGEIHLLVTDVVMP
                     EMNGRDLATQLVSMRPHLKRLFMSGYTADVIADHGVLDGGVEFIQKPFSTRELAAKVR
                     EVLD"
     gene            complement(8690..>9111)
                     /locus_tag="VFD74_02205"
     CDS             complement(8690..>9111)
                     /locus_tag="VFD74_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48399.1"
                     /translation="VRQLPDSIWYEVQMGGITGWVSSAFVAYSGAVDDITAQVIQNLG
                     GTAPSAETMVDLGLIVAEAMASDDPPSRIVVTVAPHVGDLGEITYDGIGLGDDALRGY
                     RLHVFGQPDESGEGFSLKSVESTLLCARGVTADGLAI"
BASE COUNT         1778 a         3108 c         2890 g         1335 t
ORIGIN      
        1 catttggtcc gagatcgaag cgacacccac tatgtgcaag gcagactcac acgtaagcta
       61 ccgaccgagc gtcgccgcta tgccgaagtg gtcggaggcc gtccccgtgg tgacgcgtac
      121 gtccgtgaga gaggaccccg acggcccggc caggtcagga gacacccaga tgtagtcgat
      181 acgctggaac ggctcgtcgg acgagtacgt gtagcctggc gcgatcccgg cggcctgcag
      241 tatctccacc aacccggcgt cccgcagcgt ctcgatctcg ggggatccgg gctcggcgtt
      301 catgtcgccc atcaccacca ggtccttggc ctcgccccac gcctgcagca gggactccga
      361 ttccagcacg cgcacctggc tgccgtcctc ggtgtgatgg tagtgggtgg cgacgacgtc
      421 cagagacctg ccgccgccca cgtcgatctt cgccttgatg tacccgcgct gcagcagcag
      481 ctcgggcgtg ggcagcgacg ccaactcgct ctcctcgata ggtaggcggg taagcagcgc
      541 attgccccag agaggtccgg ccgtggggcc gtagacggcc ggcatgccca gccgcgcctg
      601 caaccagccc agagagtcga ccgtcccatt gatcacccaa ccgcgggaca cctcctggag
      661 ggccacgatg tcggggttct cggcctcgat cacgcaggcg agtgcctcca gatcgaggcg
      721 accgtcggtg gcgaagccgt tgtggagatt gagagtcatc acccgcacgg gaaggccgtc
      781 accctccctc gcctgggccg gctcgaagac cgcccactgg atcagcggcg ccacggcgat
      841 caccgccacg ataagcgccg gcatcagccg ccggatccgg gagggaaggc ccacgggaag
      901 tgagtccgcg ccgttggctc ggtcgagtcg acgccgcccg acgatcgccc agacgccgca
      961 gactccaacc aggatagccg cgaagggggg cagtacatcc gccgagaagg gcagagcgat
     1021 gtcgtaaccc gagtacgcca ggaacaggat gataaccatg atcagcatgc ccacgccgtg
     1081 gaacggagag agcctcccgt acccgcccaa cgatccgctg ggtggcacgg tcgagaccct
     1141 tccggctccc accgacccca ccaccaccat cagcaggtag gccgtcgcca cctggccggt
     1201 cacgaacaac acggctgcgt ccatgccctc gggcgccgcc gggatcaccg acagcacgag
     1261 caagacaccc gccgccatcg ccagcgcccg ggcgaccacg gccaccggca ccgattccgt
     1321 cgaacctccg gccccgcgcg tcacgggcgg ggtaccctgc atgacgtaga gccaccccgc
     1381 ggccacaagt cccagcgcgt gggccacgac ggcgaggaac gcggcgcccg gcaccgagag
     1441 acccgtcagt gtgcccaggc gggcgacgtt ctggaacagc agcatctcga gcgccaggat
     1501 gggtccgaac gctatccacg gaccagcgag cagcctgccg cgggccgacg ccgcgcgaga
     1561 ccctgccccc accttcagac gatcgtggta ggccagatgg gtttgcagga gcgccaacac
     1621 cagcaccacc agaagtacga gccaacccgc acgccacgcg agatcgtagg tctggagcac
     1681 tccgttcagg gccgtgtcga gccccaagcc ggcaagtatc ccgagcgcat acgcagccgt
     1741 ggaccccgaa ccgatcccgc cgcccgccgt gcgccaaccg gccaacacga gacccgcggg
     1801 cagatagagc aggaacagca gtgtgcccac cacggccaat atcaggtcga ccgccgggtc
     1861 ccatacccac gcctggatga gcagacgggc gaggcccagg ccgatggccg tgaacatggc
     1921 cagccgccgc ggtccgagag ccttcagaag cggccccacc aagaaggagc cggcgaacag
     1981 gacgatggcc aacacgccga ggtggagggc ctgaagcccg aaacgatctc ggagcacgta
     2041 caccagcagg ggtagcagca cgcgcaacac ctgcatgccg aacatgacag tcagcgcagg
     2101 caggagtatc gacgtgcgga atagtgggaa ccgccgcccg cccggcaccg gcgcgtagta
     2161 ctcggtcgcc gcctgcactc tcttccacat gctcgtccct ccaagtatcg atcagcagaa
     2221 cagtccccta cccagtgaca tacggttcgc acagatgtgt gcctgagcga cgccctcggg
     2281 cgggtcgccc gcagacggcg cctcagcgcc tctcgaacag cctccacaga atggatgccg
     2341 cctcgccgcg tgtcgccggc gcccgcggat cccacgcagg accgaatccc acgagcccat
     2401 caagcaggct gttcgcctcg gcggtgcgga tggactcgtc gtgggtgaga tcgaagcttc
     2461 ccagagtgct tcgaaagtcg gacggcgccg cggagagcgc ggtcggcacg gcgttcgccg
     2521 ccgcccgcac caccacggtg atcgcctgag cccgggagat ctcggcgtag ggggcgaaca
     2581 gaccaccgcc gattcccacg aaagcaccgg cgtccgccgc cgcggccacg tactcgtggg
     2641 gatacagatc gaccgggtca tcgggaccga gatcggagaa tggctggggc atcgactcga
     2701 agacggtcag tccgagggtc ctcaccacca tcttggcgag ttcggcgcgc agcacgggag
     2761 cgtctgggtt gaactccaag gatcccccgc ctcccccata gccggccacg acgcctcgag
     2821 aggccaaccc cgcgacagcc aacgcgtagg cgtgggaaga aggaacgtcg ttgaagaccg
     2881 gaagagtcgg cgccgcggtc gacacgacgg tggacacgat cgtgctggtg gtcgacaccg
     2941 tggggacggc agagtgcggg gtgccggagt gcgtcgtctg gaacgcgccg gcggtcacgc
     3001 tctccacccg aggaagtccg aggaggtcgg actgcggggc ctcttcgctg tgcgaacctg
     3061 ccgagaacag ggggctgccg acgccggggt ggaagtcacc ccgctccgga tccacgagag
     3121 acggttcggc cacgagattg ccaacgccga gcgacgaagc caggtcatcc ggcccgaagc
     3181 tgcgcgctcc cgacaccacg atccgatcgg ggaagcccca gaacacattg ttctccgcgc
     3241 gaacgcgcgc cccagccggg aagtcgagcg gtcccggttc ccggtagaac aggttgttga
     3301 gaagggtcac atccatatct tgacccgcag tcgcaccgac accgaacgcc gctgcgtacg
     3361 gcccgccccc agcgccgccg ctcgtcgcag cgttccctcc tgcggtgcct cccgcggctc
     3421 caccaccacc ggcccggtcg tggtaggcga acgtgcaatt gaccacgcgg taggaaccgc
     3481 tgcctgtggc cagttgagcg tccccgcccg cgtcgaacac cagggagtcg accacgtctc
     3541 ccccagccct gagcgtgata ccgctccgtc cgacacgccg gacgatagtg ctcacgaccg
     3601 ccgagcgcgt ggccccaagg tcgatcccat cgccgcggcc cccctccacg atggagttct
     3661 cgatgagcac gttgtctccc gccccgatga caacgccccc ggcggaggcg gcgatcgtgg
     3721 tggtcccgac gcccgtgccg gccccgacgc cggcccccac ggcgtccctg gcggtcgccg
     3781 cggccgcgtc agccatgctc acgtgcagcc cgacgatatg gacgttccga ccgcccgcca
     3841 cgccgaacgt caacccaacg tcaccgatgc cgagcaggct cacgcgccgc acgagcaagc
     3901 cgtcgacgat cgtgtctccc gggaggtagt cgctggacat ccccgccggc gccccctcca
     3961 ctaccaggtc ttcgaagacc agccgctcga cggcgaccgg gttgccgtag tgcacaccca
     4021 cagagtcgaa gccggcgatc gtcaggccgc gcaccaacac atcgtgctgt cccggagcgc
     4081 tcaccgccag gccgtagccc gcatccgggc cgtccgcctg caccaccacg accccgtcgc
     4141 cggcggcggc caacgagaat cgagactgcg tcaccgtgag gccgccgctc gggtacacac
     4201 ccgcatgcac gatgactcga tcgccgtcgg cggccacgga gagggcgcgc tcgatgcggg
     4261 cgaagggcag ttcactcgat cccgggccgg agtcgtcacc gccggaggca acgtagtagg
     4321 ccgtgccgtc gggatcgctc ggatcgtcgc ctccggggaa ggcgtcgtgg tccacgcggg
     4381 cgatgcgctc gcccgccggc cgctcgagca gcagggcgtc gtcgctgccg gggtcgggtg
     4441 ccgcgaccgc tcgcggttca gtggagcttg tcgcggcccc cgcggcgggc agggtggtca
     4501 cgacggacac gaccggaccg gtgccggcac cctgctcggg agcccgaggt gcctcgccgt
     4561 atccggtggc ccgcacggcc atgccccccg ccagcacggc gaggaagaag acggtgccta
     4621 tgagccaacg tttcacgatg tacgagaacc tcagggaaga gagggtcagc gtcgatgaga
     4681 ccaccgacgc gaccaccgac gtcaacaccc acgtcaccac cgatggcgcc accaccgcgg
     4741 gacgctaccc cccaacggtg cggccacgaa ggcggcaagc cacctcgccc aagctaccac
     4801 gcccgcgtgc caggtgtcat cgacggccca gcgacggtcc cttgcgcctt cacagccacc
     4861 ccgggaagat gacgctcccg gcgccctctg ctagaatacg cggacgcgcg ggatggactt
     4921 ctgaggccgc ggtcgaagaa gccggtcaag cgtggggcat cgcaccctgg aacagggacg
     4981 ccgcccgtgc aaggagaagt ggccgagccg gctgaaggcg ctcgcctgct aagcgagtgt
     5041 aggagtgaat gctcctaccg agggttcgaa tccctccttc tccgccagtt gcgcctgtag
     5101 ctcagttgga tagagcgtcg gtctacgaaa ccgaaggcca caggttcgaa tcctgtcagg
     5161 cgcgtaggag aaggaccccc gggcatcggg ggtttttgtc cttctatctc gaaaccagac
     5221 cggcggccac atcccagaat ccgccccttc cgatgctcac cgatcctcac cgatgctcgg
     5281 cgtgtgcgcg gtcccggtac acgggcaaca cctcacttct cctcaggggg acaaaggaat
     5341 gaatagaatg aatcctaagg ttgatgagta tttgagcaaa gcgacaaagt ggcaggaaga
     5401 attcgcgcaa ttgagaacga tccttcttga ctgtgagctg accgaagaag tgaagtgggg
     5461 ctggccctgt tacatgtttc agatgagcaa tgtagtcttg ataaacggat tcaaggaata
     5521 ctgtgcgctc ttgttcttca aaggtgcttt gctaagtgat gtcaacggca ttctcaacat
     5581 gattggggag aatacgcagg cggggcgcca gattcggttc accaatgtag gcgaaatagt
     5641 tgcacttacg cccatcctgc aggcctacat ctatgaagca attgaagtgg aacgcgcggg
     5701 gttgcatgtg acgttgaaga agaatataga catcgtattt cctgaggaat ttcaaagaag
     5761 attggatggc gccccggcct tgaaaaccgc ttttgatgca ttgaccccgg gacggcaaag
     5821 agcatacaac atgtacttct ctcagcccaa acaatccaag acacgggaat caagggttga
     5881 gaagtgtgtg gagcgaatcc tcgacggcaa gggattgaac gatcagtagt tctgtcttgc
     5941 agtgacctag gtccacttgg gcgcccagga gccctcactt cagtccaaca cctctctgac
     6001 cttcgctgcc agttcgcgcg tggagaaggg cttctggatg aattccaccc ccccatcgag
     6061 cacgccgtgg tcggcgatga cgtcggcggt gtagccggac atgaacagac gcttgaggtg
     6121 cgggcgcatg gacaccagct gcgtcgccag gtcccggccg ttcatctcgg gcatgaccac
     6181 gtcggtgacg agaaggtgga tctctccctc gtactcccgg gtcaggcgga tagcctcacc
     6241 cggaccgccc gcggccaaca cggtgtagcc ctcgatctca agcatgatcg cggtcatctc
     6301 gagcagggcc ggctcgtctt ccaccaagag gatggtctcg tggccccgag cggtcgagac
     6361 cgttgaggct tccgttctcg gctggtcggt ctcggtcgtg cggggtaagt agacggtgat
     6421 cgtcgtaccc tcgccccgcc cgctgcggac atcgatgaat ccgccgttct gcttcacgat
     6481 gccgtacacc gtagccaggc cgagaccggt gccggtgccg acgcccttgg tagtgaagaa
     6541 cggctcgaag gcgtgggcca gcgtctcttc gtccatgccg cagccgtcgt cggcgacggt
     6601 catacgcgcg tactccccgg gcgccgagcc cgggtgatgg ccgcagtagg cttcgtcgaa
     6661 ggtgctgcgc ccggtctcga tggtcaatct gcgtgcaccg gcgtcccggg cgttgacgca
     6721 gaggttggcc aggatctggt cgatctggga ggcgtccacc ttgaccggcc acagctccgc
     6781 acccggctgc caattgagat cgatatcctc accgatgagc cgttccagca tgctcagcat
     6841 gtccgcaacg gtctcgttga ggttcagcac tcgcggaacg acggtctgtc tccgagcgaa
     6901 ggccaagagt tggcgggtga ggtcggcaga gcgttcggct gcgcgccgga tctcggtaag
     6961 gtccgcatgg agcggctgtc cgggaccgac gacctccaga gccatctcgg catggcccaa
     7021 gatcaccccc aacatgttgt tgaagtcgtg ggccacaccg cccgccaagc ggcccaccgc
     7081 ctccatcttc tgagcctgtt ggagttgggc ttcgagcccc tcccgctcct cctcggcctc
     7141 cacgcgctcg gtgacgtcgc gagcgttgat cacaatccga tccagagcgg ggtcatcaac
     7201 caaggcgttg acgaatacct cgatcgtccg gaacgaaccg ttgcggtggc gcactcgcag
     7261 ttccgtgggc cggtcggctc gaggaagggt ttgcgggcga gcggcgatcg agtccagcat
     7321 cgcgggtcga tcatcggcgt ggatgaagcc tacgagactc tctccaacga tctcctccgg
     7381 cgtgaacccg agcatccgtt ccaccgaagg gctcacatac cggcagatgc cgtcgggagc
     7441 cagctcgatt gtcaggtcga ccgccttctc gatcagggtg cgaaacctct cctcacgctc
     7501 gaacagcgtg gtctccgcgt gcctgcgctc agtgacgacg gcgcccacca gcaatccgac
     7561 catgcagttg acgatcatca gcagctgcag ctggccgagg agccccaggt cgagtcgaaa
     7621 gagccacatc gccagcgtca tcccgaagtt cgacgccacg atcccggtgg tgactccctt
     7681 gaagccgtga tcgagggcga tccagatgag cggcaaggtg acaagatacg ttggccggaa
     7741 ctcctccagc acatgcacac caaagaccca gtagaacatc aacacgaggc tgaacgcctg
     7801 cccgacgaca gacggagtca ggcgcagacg tagccggcga ccggacagcg tgacccgccg
     7861 ccgcccttcc gcgaaccgct ccaaccacgg catcacgtgg acaagcagga acggggtgac
     7921 ggtcagaacc cccaccatct caccgatcca ccagaggaag agagcacgga agaactcgct
     7981 tcgcggaagg ctgctgctca gagtggaact cgaaacagag agcacagcga ggagcgcgga
     8041 gacgagcgcg gccgtgacaa ccagacgagt cacgtctcgc gatgttcgca ggtgccaatc
     8101 aaagcggatc cggtgacgca ggagcgcggc ggcggcgccg tagatcgaag acacgatcac
     8161 cgcccacaac aagagcgcgt agggggactg cggcatgcgg tagatgaaga ggctcccgaa
     8221 gaagtagacg atcgtcaccg cgggcaccaa cctcacgccg aacaccaaca gaagggcgta
     8281 gataaggccg gcgggcgggt accaggcgac caccccacgc agctcttcga actgttgcga
     8341 cgcgaagtcg agaatgatga aggtcaacag gtacacggcg acgatcaacg cggtgcgcac
     8401 cggcaggcgc gggtggcgcg agggatcgcg aggcgcgggg gaagacggag cgccgggtgt
     8461 gccagacgag gccatcatcc ggagacggac ccgcctcggg cccgcgaccg gccttgccgc
     8521 tcagcgcacg ctgtgcctag aaagatgatc gctgcctcgc ttccgtgctc atcgagccgg
     8581 aacttctctc gggacgaata gtacatctcc tcaagggacg cgcgggcacg gctgcggcgg
     8641 ttccgtccga gccggcaatg gcgcgcggca cgccgccgga aacgcacccc tagatggcga
     8701 gaccgtccgc ggtcactcca cgagcgcaca gcagcgtaga ctccacgctc ttcagggaga
     8761 agccctcacc gctctcgtcc ggttggccga acacgtgcag gcgataccct cgaagcgcat
     8821 cgtcgcccag tccgatgccg tcataggtga tctcgccgag gtcacccacg tgcggagcca
     8881 cggtcaccac gatgcgcgaa ggcggatcat ccgaagccat cgcctcggcc actatcagcc
     8941 caagatccac catggtctcg gccgatggag ccgtgcctcc gaggttctgg atcacctgcg
     9001 cggtgatgtc gtcgacggct ccggagtagg ccacaaaggc gctgctcacc cacccggtga
     9061 tcccgcccat ctgcacctcg taccagatcg aatcgggaag ctgccgcact c
//
LOCUS       DASRMJ010000075         7545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_367159, whole genome shotgun sequence.
ACCESSION   DASRMJ010000075 DASRMJ010000000
VERSION     DASRMJ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367159"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2304)
                     /gene="glgP"
                     /locus_tag="VFD74_02210"
     CDS             complement(<1..2304)
                     /gene="glgP"
                     /locus_tag="VFD74_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZK48400.1"
                     /translation="MKAAHRFHVHPAIPERLACLRDLAYNLRWTWDHDTLGLFTHVDP
                     SLWQECGHNPVLLLNRVPQGRLEEAAADDAFLAHFDRVCAAHQAFLTEPGWFRRRHPE
                     SSDLTIAYFSMEFGLTECLPIYSGGLGVLSGDHLKSASHLDIPLVGVGLLYQKGYFTQ
                     YLNPDGWQLEQYPVNDFTSLPIESVCAENGKPLFVHVDLAGRRVSIRVWKAQVGRIPL
                     FLLDTNVRENHPQDQDVTDELYGGGPEERIRQEIVLGIGGMRALRAMGIVPQVCHLNE
                     GHSAFLSLERVRQLRIEAGLDYQSARQVTGAGTVFTTHTPVPAGFDLFSAELMTKYFG
                     TYVKDLGLEMEELLSRGRTVPSDASEAFNVAVLALRHSPRRNAVSRLHRSVTARMMRA
                     GWPDLPESEMPIEYVTNGVHTRGWVASDMAALFDRYLGPRWREDPADPSAWSRVDQIP
                     DEELWRTHVMQRERLVAYTRTQLQAQLRRRNAPPGELEAAGEALRSDALTIGFARRFA
                     TYKRATLLLREVDRLKVLLLDERRPLQVIVAGKAHPRDDAGKELIRDIVRFAKAEGVQ
                     HRLVFLEDYDLAKSRALVQGVDVWLNTPRRPLEASGTSGMKVVGNGGLNLSILDGWWD
                     EGYRRGVGWAIGSGEEISDLEYQDRVDAQSLYSLLEQEIIPLFYDRGTDGVPRGWLAM
                     MKASIRELTPAFSTVRMVREYTERFYLPAADQFSRLAADDYAKARQVAAWKGRVREAW
                     GEVAIVTVRGGDGEEVRAGASVPIEVQV"
     gene            2549..3037
                     /locus_tag="VFD74_02215"
     CDS             2549..3037
                     /locus_tag="VFD74_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2696..2698,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZK48401.1"
                     /translation="MSEPLAIGTSAPDFTLPSQLGDLVTLSSFHGNSGKNTVLFFYPL
                     DWTPVUSRELPIVQAHQGRFGELGAHVLGISCDSLASHRAFADASGGIDFPLLSDFWP
                     HGRVTREYGVLDEEHGHPRRSAFVIDRDGVIRWAYHAELGEQRDLSIILEVLEDLHDQ
                     RR"
     gene            complement(3113..3949)
                     /locus_tag="VFD74_02220"
     CDS             complement(3113..3949)
                     /locus_tag="VFD74_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK48402.1"
                     /translation="MTTREIIRIDESLCTGCGECVPACAEGALEIIDGKARVVAEVLC
                     DGLGACLGECPEGALTVEVREADEFDEVAVERRLREVGRVPSAAAVTPHVTAAPHMGC
                     PGSLARELRPLPTAAVATAGTAASGAADAGASGVHSERASEVDSERASESLLQNWPVQ
                     LTLVNPNAPFLRHSDLLIAADCAPAAYRLFHEDFLEGRSLVIACPKLDDAQAHYEKLT
                     LLLQTATPRSVSVVRMEVPCCSGLVSLTRAAVESAELDVRVEEIVIGVGGERLTPQAA
                     VR"
     gene            4202..4489
                     /locus_tag="VFD74_02225"
     CDS             4202..4489
                     /locus_tag="VFD74_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48403.1"
                     /translation="MALTLRQRMLVHLVVQRFDRETQTTTLDPQALSSELSASAEDVS
                     ADIDALVQQGYVERVGHVSAPGSQAAGERRPSGPWILNPTDRAVLSAMGLE"
     gene            complement(4523..5143)
                     /locus_tag="VFD74_02230"
     CDS             complement(4523..5143)
                     /locus_tag="VFD74_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HZK48404.1"
                     /translation="MRVIEIFDSLQGEGAWAGVPTAFVRFAGCNAADLGLGCTAWCDT
                     EHSWDPEGGHEMSPAEVAAALASSALRRVCLTGGEPLLQGEEFALLVTLLQDRGLTVH
                     VETNGTLDLPGGVRPGWVTVSPKPPHYVVSPGLTGAVDEIKVVVDEAFRADVVEGLSR
                     DHPTAVVSLQPEWSRFRETAAEATAAAMAHPDWRVSLQLHKVLGIR"
     gene            complement(5173..5535)
                     /gene="queD"
                     /locus_tag="VFD74_02235"
     CDS             complement(5173..5535)
                     /gene="queD"
                     /locus_tag="VFD74_02235"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HZK48405.1"
                     /translation="MRTTITKIFTFDSAHSLPEHLGKCRNVHGHTYTMEVAVEGPVDH
                     GGPTDGMVMDFADLRARVAELVVGPLDHRYLNDLFDFAPTAEALASWAMLTLREAGLA
                     VVRVRLWETPTSYAEVSI"
     gene            complement(5539..6210)
                     /gene="queC"
                     /locus_tag="VFD74_02240"
     CDS             complement(5539..6210)
                     /gene="queC"
                     /locus_tag="VFD74_02240"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZK48406.1"
                     /translation="MNASADYLLLSGGLDSTTLAAWVMNKHPSSRITAVTFHYGQKHA
                     VESEAAKRVAAHYGFEHRLAELPRISGSALTDSDTPLPEGRDLAAVTGVAPSYVPARN
                     LLFLAFLASLADADGPARLWLGVHRDDHAGYPDCRPEFVRAADEAVCLGTQFGLRVEA
                     PFVEWPKERILRWGLEHDVPYALTHSCYQGLRPACGVCDTCQARMAAFRLVEAQDPIP
                     YATRS"
     gene            complement(6207..6590)
                     /gene="queF"
                     /locus_tag="VFD74_02245"
     CDS             complement(6207..6590)
                     /gene="queF"
                     /locus_tag="VFD74_02245"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZK48407.1"
                     /translation="METEKKYGTLAIESSALVAWPNPSPHRRYLVSMDIPEFTCVCPQ
                     SGFPDFATIKIRYQPDGLVVELKSLKLYVNRYRDVAISHEAVTNKILDDLVELLQPHW
                     MRVTGDFNVRGNIKTIIRAEHGVRG"
     gene            complement(6803..7009)
                     /locus_tag="VFD74_02250"
     CDS             complement(6803..7009)
                     /locus_tag="VFD74_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZK48408.1"
                     /translation="METGKVKWFNDSKGYGFIERTEGEDVFVHFSAVTGDGFRTLTEG
                     EAVNFEVVEGPKGLQAANVSRLGA"
     gene            7282..>7545
                     /locus_tag="VFD74_02255"
     CDS             7282..>7545
                     /locus_tag="VFD74_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZK48409.1"
                     /translation="MTDDVDIRKILVGYDGSESSERAVTLACSLARKYGAAITVVHAF
                     PHLPRLSAPSQDDTREIHAARRFVEVLVKRLSHEEVEVVPDVLE"
BASE COUNT         1330 a         2538 c         2449 g         1228 t
ORIGIN      
        1 cacctgcacc tcgatcggca cggaagcgcc ggctcggacc tcctccccgt cgcctccccg
       61 cacagtgacg atcgccacct ctccccacgc ctcccgcact cgacccttcc acgccgccac
      121 ctgtcgggct ttcgcatagt cgtccgccgc caaacggctg aattggtccg ccgccggcag
      181 gtagaagcgc tccgtgtact cgcgcaccat gcgcaccgtg gagaaggcag gagtgagttc
      241 gcggatcgac gctttcatca tcgccagcca gccccgcggg actccatccg ttcccctgtc
      301 gtagaagagg gggatgatct cctgctcgag gagcgagtag agactctgtg catcgacgcg
      361 atcctgatac tcgagatccg atatctcctc gccactcccg atagcccacc cgacgcccct
      421 ccggtagcct tcgtcccacc aaccgtcgag gatcgaaagg ttgagaccgc cattgcccac
      481 gaccttcatg ccgctggttc cgctggcctc caacggccgg cgcggggtgt tgagccagac
      541 gtccacgccc tggacgaggg cacgcgactt ggcgagatcg tagtcttcga ggaagacgag
      601 ccggtgctgg actccctccg ccttggcgaa gcgcacgatg tctcggatga gttccttgcc
      661 cgcgtcgtcg cgggggtggg ccttgcccgc gacgatcacc tggaggggcc ggcgttcgtc
      721 gagcaacagg accttcagac ggtctacttc gcggagcagg agagtggccc gcttgtaggt
      781 cgcgaaccgg cgcgcgaaac cgatcgtgag ggcatccgag cgaagtgctt cccccgcggc
      841 ctcgagttcg cccggcgggg cgttccgccg ccgaagctgg gcctgcaatt gcgtgcgtgt
      901 gtatgccacc aagcgctccc gctgcatgac atgggtgcgc cagagttcct catcggggat
      961 ctgatccacc cgactccagg ccgacgggtc ggcgggatcc tcccgccagc gaggaccgag
     1021 gtagcgatca aagagcgcgg ccatatcgga ggcgacccat ccccgggtgt gcacgccgtt
     1081 ggtgacgtac tcgatgggca tctcgctctc cggcaggtcg ggccagccgg cccgcatcat
     1141 gcgggcggtg acactccggt ggagtcggct gaccgcgttt cgacgcggcg aatgccggag
     1201 ggccaagacg gcgacgttga acgcctccga cgcgtccgaa ggcacggtcc ttcctcggga
     1261 aaggagttcc tccatctcca gcccgaggtc cttcacgtag gtgccgaagt acttggtcat
     1321 gagctcggcg ctgaacaggt cgaaaccagc gggcacgggg gtgtgcgtgg tgaacaccgt
     1381 gccggcgccg gtcacctggc gggctgactg atagtcgagg ccggcttcga tgcgcagctg
     1441 ccgcacgcgc tccaaggaga ggaacgcgga gtggccttcg ttgagatggc atacctgcgg
     1501 gacgatgccc atggcgcgga gcgcgcgcat accaccgatc cccagtacga tctcctggcg
     1561 gatccgctct tccggaccgc cgccgtaaag ctcgtcggtg acatcctgat cctgcggatg
     1621 gttctcccgc acgttcgtgt ccagcaagaa gagggggatg cgcccgacct gagccttcca
     1681 cacgcggatg gacaccctgc gcccggccag gtcgacgtgg acgaacaggg gcttgccgtt
     1741 ctcggcgcac accgactcga tcggcagcga agtgaagtcg ttgacggggt actgctcgag
     1801 ctgccatcca tcgggattca gatactgagt gaagtagccc ttctgataga gcaatcccac
     1861 ccccaccaag gggatgtcca ggtggctcgc cgacttcaag tggtcgccgg agaggactcc
     1921 cagccccccg gagtagatgg ggagacactc ggtgagcccg aactccatgg agaagtaggc
     1981 gatggtcagg tcggaggatt ccggatgccg cctgcggaac caccccggct ccgtgaggaa
     2041 ggcctggtgt gccgcgcata cgcggtcgaa gtgggcaagg aaggcatcgt ccgccgccgc
     2101 ctcttccaga cgtccctggg gcaccctgtt cagtaacagc acggggttgt ggccgcactc
     2161 ctgccacaga gagggatcga cgtgtgtgaa gagtccgagg gtgtcgtggt cccatgtcca
     2221 gcgcaggttg taggccagat cgcgcaggca ggccaaccgc tccgggatcg cgggatgcac
     2281 gtggaaacgg tgcgcggctt tcatcgaccc tacctcccgc ctgcgcctgg gacacggtcg
     2341 cggctgcccg attcaagacg gcgccgcttg gccaatctta cccgaatgtc gcccgaatac
     2401 gcagcacgcc tcagccaccg gcgacgagtc cggggcacac ggatgtttca accttcgtgc
     2461 gccggcggaa ccatgagcgc aagggggcgc ggtccgtcgg cgacagccct cgcgcgcatc
     2521 ctcggctctg actcgtcagg agtgcaccat gagcgaaccg ctggcaatcg gcacctcggc
     2581 cccggatttc accctccctt ctcaactcgg cgacctcgtg accctgtcct cgtttcacgg
     2641 caactcgggc aagaacaccg tgctcttctt ctacccgctc gactggacac ccgtctgaag
     2701 ccgcgagctc cccatcgtgc aggcccacca gggccgattc ggggagctcg gagcccacgt
     2761 gctgggcata agctgcgact ccttggcgtc acaccgcgcg ttcgccgacg cgtccggggg
     2821 catcgacttc cctctgctga gcgacttctg gcctcatggg cgtgtaacac gcgaatacgg
     2881 ggtgttggac gaagaacacg gccatccccg acgcagcgcc ttcgtgatcg atcgggacgg
     2941 tgtcatccgg tgggcctacc acgccgagct gggcgagcag cgcgacctgt cgataatcct
     3001 cgaggtgctt gaagacctgc acgaccagag gaggtagagc agtgccggcc gcaggaagca
     3061 gtgccggccg caggaaacgg ccggcacccg aaggagaagg ccccgagtgg ggctacctca
     3121 cagccgcctg cggcgtcagg cgttcgccgc cgactccgat gacgatctcc tccacgcgaa
     3181 catcgagttc ggcggactct accgcagccc gggtgagcga gaccagtccc gagcagcacg
     3241 gcacctccat ccgaaccaca ctcaccgaac ggggcgtcgc cgtctgcaag aggagcgtca
     3301 acttctcgta gtgggcctgg gcgtcgtcca gcttgggaca cgcgatgacc agactcctgc
     3361 cctcaaggaa gtcttcgtgg aacagccggt atgcggccgg cgcgcaatca gcggcgatca
     3421 agaggtctga gtgcctcaag aacggcgcgt tggggttcac tagagtcagc tgcaccggcc
     3481 agttctggag taggctttcc gacgcgcgct ccgagtcgac ttccgacgcg cgctccgagt
     3541 ggacgcccga cgcgccggca tccgcagccc ccgaggccgc ggtccccgcg gtcgcaacag
     3601 cggccgtcgg cagaggcctc agttcacgcg ccagcgaccc gggacagccc atgtggggcg
     3661 ccgcggtgac gtgaggcgtg acggcggcgg ccgacgggac gcgcccgacc tcgcggaggc
     3721 ggcgttcgac ggcgacttcg tcgaactcgt cggcctcgcg cacctccaca gtcagcgccc
     3781 cctcggggca ctcgcccaaa caggctccca gaccgtcgca aaggacctcg gccaccaccc
     3841 gggccttgcc gtcgatgatc tcgagcgcgc cctcggcgca cgcgggcacg cactccccac
     3901 atcccgtgca cagggattcg tcgatccgaa tgatctctct tgttgtcaac cgtgcctcct
     3961 cttgccgatg ccaagcctac gtgtctcgcg cgaccgccct ccgcgcgaag actccaccgc
     4021 tctcgtaccc ggctccttgc agtgtaaaca ctgctatcgt agtgtttatt ccgaccagcg
     4081 tcaagcctgg cccccgtcga cccagaggga gtgtatcctc atgctctgcg ccctcctcgc
     4141 ggcactcgcc gaagagtcgg gcgcgggatc cgagagcgac catgcaccaa ggaggcggac
     4201 catggccctg acgctgcgtc aaaggatgct cgtgcaccta gtggtgcaac gcttcgaccg
     4261 agagacgcag acgaccacgc tcgatcccca ggccctgtcg tccgaactat ccgccagcgc
     4321 tgaggacgtg tccgccgaca tcgacgcgtt ggtgcagcag gggtacgtag agcgcgtcgg
     4381 gcacgtgagt gccccgggat cgcaggctgc gggcgaacgt cggccatccg gcccctggat
     4441 cctcaacccg accgatcgcg ccgtgctctc cgccatgggc ctggaataga ggatcgaagc
     4501 cgcgaaggcg cggcctccga ggtcagcgga tcccaagcac cttgtgcagt tgaagcgaca
     4561 cgcgccaatc cgggtgcgcc atcgcggcgg ccgtggcttc ggcagccgtc tcacggaatc
     4621 tcgaccactc cggctggaga ctgaccacgg ctgtggggtg gtcgcgggat agtccctcga
     4681 ccacgtccgc gcggaacgcc tcgtcgacca ctaccttgat ctcgtcgacc gcccccgtca
     4741 gccccgggga caccacgtag tggggaggct tgggactcac ggtgacccaa ccgggacgga
     4801 ctcccccggg caggtcgaga gtcccgttgg tctcgacatg gacggtaagg ccccgatcct
     4861 gcagaagcgt gacgagaagg gcgaactcct caccctgaag tagaggttca ccgccggtaa
     4921 ggcagacgcg gcgcaacgcc gaagatgcga gagccgcggc cacctctgca ggagacatct
     4981 cgtgcccccc ttcgggatcc cagctgtgct ccgtgtcgca ccacgcggtg cagccgagac
     5041 ccaggtccgc ggcgttgcag cctgcgaagc gtacgaacgc cgtgggcacc ccggcccagg
     5101 ccccctcacc ctgcagagaa tcgaagatct cgatgacgcg cacgcccgac tcccaggatc
     5161 tcgacgcggc cgctagatag agacttcggc gtagctggtg ggagtctccc agagacggac
     5221 ccgcaccacc gccaggccgg cctcgcgcag cgtcagcatg gcccacgacg caagagcctc
     5281 cgccgtgggg gcgaaatcga agaggtcgtt caggtagcgg tggtcgagcg gacccaccac
     5341 cagttccgcg acgcgcgccc gaagatcggc gaaatccatc accatcccgt cggtcgggcc
     5401 gccatggtcc accgggcctt cgacggcgac ctccatcgtg taggtgtgcc catgcacgtt
     5461 gcggcacttc cccaggtgct cgggaagact gtgggctgag tcgaaggtga agatcttggt
     5521 gatggtcgtg cgcacgggtc agctccgggt ggcgtagggg atgggatcct gggcttccac
     5581 cagacggaaa gccgccattc gggcctgaca ggtgtcgcag acaccacatg ccgggcggag
     5641 cccctggtag cacgagtggg tgagcgcata cggtacgtcg tgctcaagcc cccaacggag
     5701 gatgcgctcc ttgggccact ccacgaacgg cgcctccacc cgcaggccga actgcgtgcc
     5761 gaggcagacg gcctcgtcgg ccgcacgcac gaactcgggc cgacagtctg gatagccggc
     5821 atggtcgtcg cggtgcacgc cgagccacag ccgggcaggg ccgtccgcat cggcgaggga
     5881 agcgagaaag gcgagaaaga gaagattgcg ggccggcacg tacgagggcg ccaccccggt
     5941 gacggcggcc aggtcgcgcc cctcaggcag cggcgtgtcg ctgtcagtga gggccgagcc
     6001 cgagatgcgc gggagctccg ccagacggtg ctcgaacccg tagtgcgccg ccactctctt
     6061 cgccgcctcc gattcgaccg cgtgcttctg gccgtaatgg aacgtgaccg ccgtgatgcg
     6121 cgacgaaggg tgcttgttca tgacccacgc cgcaagcgtc gtggaatcga gccccccgct
     6181 gaggaggagg tagtcggccg acgcgttcac ccgcggacgc cgtgctccgc tcggatgatc
     6241 gtcttgatgt tgccgcgcac gttgaagtcg ccggttaccc gcatccagtg cggttgcagg
     6301 agttccacca gatcgtccag gatcttgttg gtgacggcct cgtgcgagat ggccacgtct
     6361 cgataccggt tcacgtacag cttgagagac ttcaactcca ccaccaggcc gtccggctga
     6421 tagcggatct tgatggtggc gaagtcgggg aaaccggact gcgggcagac acaggtgaac
     6481 tctgggatgt ccatcgaaac gaggtagcga cggtggggcg acgggttggg ccacgccacg
     6541 agggcgctgc tctctatggc cagagtgccg tacttcttct cggtctccat gtggagtggc
     6601 ttctcctcgg cgccaaggcg cgcggaccac ccgcgaacgc cggagtcatg tagttctaga
     6661 agggtaccac gcggccgggc cggatccgtc tcggaatggc cgggctccgg tcttcgccgg
     6721 gtgcccgtgg cgccgggaaa aagaacggcc ctgcggacga aacccgtccg cagggccgaa
     6781 aacggcctag tctgaatacc tgctaggcgc cgaggcggct cacgttggca gcctgcaggc
     6841 ccttgggacc ttcgaccacc tcgaagttga cagcttcgcc ctcagtgagg gtgcggaacc
     6901 cgtcacccgt gacagcggag aagtgcacga acacatcttc gccctcggtg cgctcgatga
     6961 agccgtagcc cttcgagtcg ttgaaccatt tgaccttgcc ggtctccatc ttgtgggacc
     7021 tcctactttg ttactccggg cctcgcccgg ggagcgacgt tgttactcgc tccttactac
     7081 tcggaagtcc ctacattgga tcgaggcgac gggtggtttc actcccgact ctggaccttc
     7141 agaactccga acgacaattc cgtagtctgc caggtttcca ggacttcgtc aagcagagcg
     7201 acggaagtcg gtcccacggt tcacaacgcc gcagcagcgg gtagacgagg acctgggcga
     7261 acggatagag aggagtctcg tatgacagat gacgtggaca tccgcaagat cctcgtgggc
     7321 tacgatgggt cggaaagctc ggagcgggcg gtcaccctgg cgtgcagcct agctcggaag
     7381 tacggcgcgg cgatcacagt ggtgcacgcc tttccgcacc ttccgcgact gagcgctcca
     7441 tcgcaggacg acacgcgcga gatacacgct gcgcgtcgtt tcgtcgaggt tctcgtgaag
     7501 cgactctctc atgaagaggt ggaggttgtg cccgacgtgc tggaa
//
LOCUS       DASRMJ010000076         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_223395, whole genome shotgun sequence.
ACCESSION   DASRMJ010000076 DASRMJ010000000
VERSION     DASRMJ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223395"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFD74_02260"
     CDS             <1..516
                     /locus_tag="VFD74_02260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK48410.1"
                     /translation="RQDELISLLYAGSDAVLANSGHEPFGLVGLEVMAVGGLAFVGST
                     GEDYAVPYLNAVVLDSDDPMEISVALEYLRMHPEVAERIRAEAQQTARSFSWSTIIED
                     TLLGKLKYVTLRQDVRAPSQAPASQAPPSPPRGGGAGEAAGAGGYAYGGESVEGGASS
                     RRARAPRFPKV"
     gene            602..>2031
                     /locus_tag="VFD74_02265"
     CDS             602..>2031
                     /locus_tag="VFD74_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HZK48411.1"
                     /translation="MSRSPDIYLALGFHDHQPVGNFPHVFEDHYRRAYLPFLDVMEAH
                     PAVPFSLHLTGPLLLWLAGRRPEYVDRLRRLCAAGRVEMKGGGFYEPILAAIPDDDKR
                     EQLERLNRYLEENLEVVPEGAWIAERVWEPHIARHLAQAGLTYAPLDDSHFLGAGLAQ
                     DDMYGYYVTEEGGFRFDLFPISKELRYLIPFQEPEATVHYLKDVAAQWRARMDAGDLP
                     PDTPPPLAVYDDDGEKFGGWPDTHEHVYGNRWLERFLVALEHEVEAGWLGLSTLGAFR
                     RRFPPLGRIYLPTASYAEMQEWSGGFYRNFLVRYEEANLLHKRMLHTSLGVARCLREL
                     ETRTASGLDEPGDLTVRRQALLTSRDRVLRSQCNDAYWHGVFGGIYLPHLRHAAYADL
                     IEAERLAGPALRPPLEVVDLDIDGFDEIFLRTQALTVLVHPRRGGTVVSLDHLATSFA
                     LLDTLRRRREPEHAALEELAAARASGG"
BASE COUNT          318 a          727 c          657 g          329 t
ORIGIN      
        1 agacaggacg aactcatctc gctgctgtat gccggctccg acgcggtgct cgccaactcc
       61 ggccacgagc cgttcggtct ggtcgggctg gaggtcatgg ctgtcggcgg cttggccttc
      121 gtgggctcaa ccggggaaga ctacgccgtg ccctacctca acgccgtggt gctcgactcc
      181 gacgacccca tggagatctc ggtggccctc gagtatctgc gcatgcaccc cgaggttgcc
      241 gaacgtatcc gcgcggaggc gcagcagacc gcccgcagct tctcctggtc caccatcatc
      301 gaggacacgt tgctgggcaa actcaagtac gtgaccttgc gccaggacgt gcgcgcgccc
      361 tcgcaggcgc cggcctcgca agcgccgccc tctccgcccc gtggaggtgg cgcgggggaa
      421 gccgcgggcg cgggcggcta cgcgtacggc ggagagtctg tcgagggcgg tgcgtcctcg
      481 aggcgcgccc gcgcaccgcg ctttcccaaa gtctgacccg gaggggcgac atgcctcagc
      541 acttggtgac ctaccgcgcc gtccaccagc cgcggcgcct gcgcgacgcc ggcggcgacc
      601 tgtgagccgc tcccccgata tctacctggc cttgggcttc cacgaccacc aaccggtggg
      661 gaacttcccg cacgtcttcg aagaccacta ccgcagggcc tacctgccgt tcctcgacgt
      721 gatggaggca cacccggcgg tgcccttctc gctccacctc acggggccgc tcctgctctg
      781 gctggcgggt agacgccccg agtacgtcga ccggctgcgc cgcctgtgcg ccgccggccg
      841 cgtagagatg aaggggggcg gcttctacga acccatcttg gccgccatcc ccgacgacga
      901 caagcgggag caactcgaac gcctgaaccg ctatctggaa gagaacctgg aggtggtgcc
      961 ggaaggcgcc tggatcgcgg aacgggtctg ggagccccac atcgcccgcc atctggcgca
     1021 ggccgggctc acgtatgctc cgctcgacga ctcgcacttc ctaggtgcgg gcctagcgca
     1081 ggacgacatg tacggctatt acgttaccga agagggcgga ttccggttcg acctctttcc
     1141 gatcagcaag gaactgcgct atctcatccc cttccaggag cccgaggcta ccgtccacta
     1201 cctgaaggac gtcgcagccc agtggcgggc gcgcatggac gccggcgatc tcccacccga
     1261 cacgccaccg ccgctcgccg tgtacgacga cgacggcgag aagttcggag ggtggccgga
     1321 tactcacgaa cacgtctacg ggaaccgctg gctcgagcgg tttctcgtcg cccttgagca
     1381 cgaggtcgaa gccgggtggc tggggttgtc cacgctcgga gccttccgcc gccgctttcc
     1441 tccgctgggc aggatctacc tccccacagc gtcgtacgcg gagatgcagg agtggtccgg
     1501 gggcttctac cgcaacttcc tggtgcgcta cgaagaggcc aacctcctgc acaagcgcat
     1561 gctccacacg agtctgggcg tcgcgcgctg cctccgcgaa ctggagacgc gaacggcctc
     1621 gggcctggac gagcccggtg acctcacggt gcggaggcag gcgctcctga cctcccgtga
     1681 ccgagtgctc cgatcacagt gcaacgacgc ctactggcac ggcgtcttcg gcggcatcta
     1741 ccttccccat ctgcgccacg ccgcctacgc cgatctcatc gaggccgaga gactggccgg
     1801 ccccgcactg cgccctccgc tcgaggtggt ggatctcgat atcgacggct tcgacgagat
     1861 cttcttgcgc actcaggcac tcaccgtgct cgttcacccg cgcaggggcg ggaccgttgt
     1921 ctcgctggac catctggcca cgtcgttcgc attgctggac acgctgcgca ggcgtcgcga
     1981 gccggaacac gcggccctgg aggagttagc ggcggcccgc gcatccggcg g
//
LOCUS       DASRMJ010000077         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_42152, whole genome shotgun sequence.
ACCESSION   DASRMJ010000077 DASRMJ010000000
VERSION     DASRMJ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42152"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /gene="uvrA"
                     /locus_tag="VFD74_02270"
     CDS             complement(<1..1807)
                     /gene="uvrA"
                     /locus_tag="VFD74_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZK48412.1"
                     /translation="MRTNEIVIVGAREHNLKDISLRLPRDRFIVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVGT
                     VTEIHDYLRLLYARIGHPHCWICGRPIEGQSKGQIIDQILALEPGTKFVVRAPLVRGQ
                     KGWHREALEHVRREGFTRVEIDGVQYTMDGQLPELDKNVRHDVSIVVDRLVMKEGVRR
                     RLSDSVETALSEGQGLLEVDVVQGEERTTLVFSENLACPEHGVSLPEMAPRIFSFNSP
                     HGACEVCTGLGSRREIDPDLIVGNPDRSIAEGVLLPWSTSSSQYYEQIITALAEKYEI
                     DIDAPWRSLPEEVRKAFLFGTGKERLYVSYFNRRGLKRSYMTTFEGIVPNLERRYRET
                     DSVYMRDKIEEYMSEVPCPACKGARLKPQSLAVTVGGLNLHELSILAVTELVGFFREL
                     QLSERERLIGGRVLKEILERLSFLDSVGVGYLDLHRTAGTLSGGEAQRIRLATQIGSS
                     LVGVLYILDEPSIGLHQRDNRKLIETLLRLRDLGNTVIVVEHDEETMLAADFLVDMGP
                     GAGEHGGEVVASGPVEAVLAEPRSITGQFLSGHISI"
     gene            complement(1858..2175)
                     /locus_tag="VFD74_02275"
     CDS             complement(1858..2175)
                     /locus_tag="VFD74_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZK48413.1"
                     /translation="MSASRLASLLVVVPLALARGGVRLPRTLLPWVFLAGLLEAVAFL
                     ALATATALGPVSVAAVVTSQFATFGVILGVVFLKERPTPWQMVGVASTIGAVSLLAGV
                     GGG"
     gene            complement(2181..>2557)
                     /locus_tag="VFD74_02280"
     CDS             complement(2181..>2557)
                     /locus_tag="VFD74_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZK48414.1"
                     /translation="LCDLVMVRLSRSGSASVALLSWVIGVGVLVMLPMALLIDGVPAG
                     SDQWLATGLAAIGGLTYVTTLYCLVRALRVGMLAIVSPLVALEGAVAALGSFALGERV
                     GRLTVAGLVLAVTGSILAKSPS"
BASE COUNT          478 a          858 c          823 g          398 t
ORIGIN      
        1 ggatagaaat gtggcccgag aggaactgtc cggtgatgga gcgcggctcc gccaagaccg
       61 cttccaccgg accgctggcc accacctcgc ctccgtgctc ccccgccccc gggcccatgt
      121 ccaccaggaa atcggcggcc agcatggtct cctcgtcgtg ctccaccacg atcacggtgt
      181 tgccgaggtc gcgcagacgc aggagggtct cgatcagctt gcgattgtcg cgctggtgca
      241 ggccgatgga gggctcgtcg aggatgtaga gcactccgac caggcttgag ccgatctgcg
      301 tggccaggcg gatgcgctgc gcctcgcccc ctgagagcgt gccggcggtg cggtggaggt
      361 cgaggtagcc cacgccaacc gagtccagga acgagagacg ttccaggatc tcttttagca
      421 ctcgtccgcc gatgaggcgt tcgcgctcgc tcagctgcaa ttcgcggaag aaccccacca
      481 gctcagtcac cgccaggatg ctgagttcgt ggagattcaa cccgcccacc gtcaccgcca
      541 ggctctgtgg ctttagacga gcccccttgc aggccggaca gggcacctcg gacatgtact
      601 cctcgatctt gtcgcgcatg tagacggagt cggtctctcg gtagcgccgc tccaggttgg
      661 gcacgatgcc ttcgaaggtg gtcatgtagc tgcgcttgag accccgccgg ttgaagtacg
      721 acacgtacag gcgctccttg cccgtgccga acaggaaggc cttgcgcacc tcctccggca
      781 aggagcgcca aggggcgtcg atgtcgatct cgtacttctc ggccaacgcc gtgatgatct
      841 gttcgtagta ctgggacgag gaggtgctcc aggggagcag cacgccttcg gcgatggagc
      901 ggtcgggatt gcccacgatc agatcgggat cgatctcgcg ccgcgagccg aggccggtgc
      961 agacctcaca ggcgccgtgc ggtgagttga atgagaagat gcgaggagcc atctcgggca
     1021 gcgacacccc gtgctcgggg caggccaggt tctcggagaa caccagcgtg gtgcgctcct
     1081 caccctggac cacgtccacc tcgagcaggc cttgcccttc ggaaagagcc gtctccaccg
     1141 agtctgagag ccggcgccgc accccttcct tcattaccaa acggtcgacc acgatgctca
     1201 cgtcgtgccg cacgttcttg tcgagctccg gcaactggcc gtccatggtg tactggacgc
     1261 cgtcgatctc cacccgggtg aagccctccc gccgcacgtg ctccagagcc tccctatgcc
     1321 aacccttctg tccgcggaca agcggcgcgc gcaccacgaa cttggtgccc ggctccaacg
     1381 ccaggatctg gtcgattatc tgtcccttgc tctggccctc gatgggacgc ccgcagatcc
     1441 agcagtgagg gtggccgatg cgggcgtaga gcagacgcag gtagtcgtgg atctcggtga
     1501 ccgttcccac agtggagcgc ggattgcgcg acgtggtctt ctggtcgatg gagatggccg
     1561 gtgacagacc ctcgatggag tcgacgtcgg gcttgtccat ctgccccaag aactggcggg
     1621 cgtacgacga caggctctcc acgtaacggc gctgtccctc ggcgtagatg gtgtcgaagg
     1681 caaggctcga cttgccggac ccggagaggc cggtgatgac gatgaaacgg tcgcgcggga
     1741 gcctgaggga gatgtccttg aggttgtgtt cccgagcgcc gacgatgacg atctcgttgg
     1801 tgcgcatgtg atgttggatt ttaccaccgg gagcggacgg aagcgggttc agcccatcta
     1861 cccgccaccc accccggcca ggagcgatac ggccccgatc gtggaggcga cgcctaccat
     1921 ctgccacgga gtcggccgct ccttgaggaa cacaacgccg aggatcacgc cgaaggtggc
     1981 gaactgcgag gtgaccaccg cggccaccga taccgggccc aaggccgtcg ccgtggcgag
     2041 cgccaagaac gcgaccgcct ccaacaggcc ggcgaggaac acccagggga gcaacgtccg
     2101 gggaagcctc acgcccccgc gggcaagcgc gagcgggacc accaccaaaa ggctcgccaa
     2161 gcgagatgcc gacacgacag tcagctcgga gacttggcca ggatgctccc ggtcaccgct
     2221 aagaccagac ccgcgaccgt caatcggccc acccgctctc ccaaagcgaa cgagccgagc
     2281 gccgcgacgg ctccctcgag ggcgactagg ggggacacga tcgcgagcat cccgactctg
     2341 agcgcgcgca ccaggcagta cagagtcgtc acataggtca acccgccgat ggccgccaga
     2401 cccgtcgcca accactggtc agagccggcg ggcacgccgt cgatcaggag cgccatggga
     2461 agcatgacca gcacccccac gccgatcacc caactgagga gtgcgacgct cgcggaacca
     2521 ctgcgggaaa gcctcaccat gacgaggtca cacagcg
//
LOCUS       DASRMJ010000078         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_408304, whole genome shotgun sequence.
ACCESSION   DASRMJ010000078 DASRMJ010000000
VERSION     DASRMJ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408304"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /gene="pstA"
                     /locus_tag="VFD74_02285"
     CDS             <1..899
                     /gene="pstA"
                     /locus_tag="VFD74_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017609101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZK48415.1"
                     /translation="RGAQGTGTSAAAHLLSEHPSPRRLWTDRAASAGMMAAVALALLP
                     VVLIFYYIIREGLGAMSWDFLTQPQPYSFRREGGGFVNGLIGTLIMVGIATAVAVPLG
                     VLAAVYLVEYGKSGILAQLIRFFADVMTGVPSVFVGLFVYTAFVVPFGFGALMGAAAL
                     AIIMLPIVVRSTEEMLKLVPVDVKNAAYALGAAKWQTVLRVVLPSALPGITTGVLLAV
                     ARAAGETAPLILTALGSLTVTTKLIGEPISALPLAIFDGARTPFAAGQARAWAGALEL
                     LAMVLVFTLLARVASSRSSIAR"
     gene            896..1678
                     /gene="pstB"
                     /locus_tag="VFD74_02290"
     CDS             896..1678
                     /gene="pstB"
                     /locus_tag="VFD74_02290"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZK48416.1"
                     /translation="MNDPVFSVRDLSVTYRGNLALKEVTLDIQPREITAIIGPSGCGK
                     STFIRCLNRMNDLVPGAEVTGQALYHGEDIYGPDVDPVTVRRRIGMVFQRPNPFPKSI
                     YDNIAFGPRISGFHGDLDELVERSLRRAALWDEVKNKLRKSALALSGGQQQRLCIARA
                     IAVEPDVILMDEPASALDPIATVHIEDLMQELKKEYSIVIVTHNMQQAARVSDKTAFL
                     YTEVDEETSSRWGILVEFGETGHIFTKPSDKRTEDYVSGRFG"
     gene            1707..2354
                     /gene="phoU"
                     /locus_tag="VFD74_02295"
     CDS             1707..2354
                     /gene="phoU"
                     /locus_tag="VFD74_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZK48417.1"
                     /translation="MRDTFHRELERLDQDVVRMGALVEAAIEGAILALAEGDLERAKR
                     VQNGDDEIDDLFIDMEKRAMAMLAQQQPVAGDLRLIVTILRVVNDLERAGDLAFNIAS
                     VVRKDLDIVKLRKVSALVYELGHASRRLLSKALDVWSEKNLELAGQMDDLDDEIDELY
                     ERLFRELFELQNETTFDVAMNLVLVGRYFERIADHAVNLAERTRYFITGNEEHLG"
BASE COUNT          408 a          826 c          800 g          414 t
ORIGIN      
        1 cccgcggcgc ccagggcacc ggcacctcgg ctgcggccca cctgctctcc gagcacccct
       61 cgccgcgtcg cctctggacc gaccgggccg cctccgcggg catgatggcg gcggtcgcat
      121 tggccctgct gccggtggta ctcatcttct actacatcat ccgcgagggc ctgggcgcca
      181 tgagctggga cttcctcacc caaccgcagc cgtacagctt tcgtcgtgag ggcggtggct
      241 tcgtcaacgg actcatcggc accctcatca tggtgggcat cgccaccgcg gtcgcggtgc
      301 cgctgggagt gctcgcggcc gtctacctcg tggaatacgg caagagcggg atcctggccc
      361 aactcatccg cttcttcgcc gacgtcatga ccggggtgcc ctccgtgttc gtggggctct
      421 tcgtatacac ggcgttcgtg gtgcccttcg gcttcggcgc gctcatgggc gcggcggcgc
      481 tcgccatcat catgctgccc atcgtcgtgc gctccaccga ggagatgctg aagctcgtgc
      541 ccgtggacgt caagaacgcc gcctacgcgc tcggggccgc gaagtggcag acggtgctgc
      601 gcgtcgtgct gccgtcggcg ctgccgggga tcaccaccgg cgtcctcctc gcggtggctc
      661 gggcggccgg cgagacggcc cccctcatcc tcaccgcgct cggctcactc accgtgacca
      721 ccaaactcat cggcgagccc atctcggccc tgccgctcgc catattcgac ggcgcgcgca
      781 ctccgttcgc ggccggtcag gcccgggcct gggcgggggc gctcgagctg ctggccatgg
      841 tcctcgtgtt cacactactc gcccgcgtgg cgtcatccag gagttcgata gcgcgatgaa
      901 cgatccggtc ttctcagtcc gcgacctgag cgtcacctac cggggcaatc tcgccttgaa
      961 ggaggtgacc ctcgacatcc agccgcggga gatcacggcc atcatcggtc cctccggctg
     1021 cggcaagagc acgttcatcc gctgcctgaa ccgcatgaac gacctggtgc cgggggcgga
     1081 ggtcaccggg caggcgctct accacggtga ggacatctac ggacccgacg tcgatcccgt
     1141 caccgtgcgt cggcgcatag gcatggtgtt ccagcgaccg aacccgttcc ccaaatccat
     1201 ctacgacaac atcgccttcg gccctcgcat cagcgggttc cacggcgacc tcgacgagtt
     1261 ggtggagcgc tcactgcgac gcgccgccct gtgggacgag gtcaagaaca agctgcgcaa
     1321 gagcgccctg gccctctccg gtgggcagca gcaacgtctg tgcatcgccc gcgccatcgc
     1381 ggtggagccc gacgtcatcc tcatggacga gccggcgtcg gccctcgatc ccatcgccac
     1441 ggtgcacatc gaggatctca tgcaggagtt gaagaaggag tattcgatcg tcatagtcac
     1501 ccacaacatg cagcaagccg cccgggtgtc cgacaagacg gccttcctct acactgaggt
     1561 cgacgaggag acgtcgtccc gctggggcat tcttgtcgag ttcggagaga ccggccacat
     1621 cttcactaaa ccgtcggaca agcgcaccga agactacgtg tcgggacgat tcggatagat
     1681 agcatgggaa agcggggcta ggtcagatgc gggacacatt ccaccgggag ttggagcgac
     1741 tcgatcagga cgtcgtgcgt atgggcgccc tggtcgaggc ggcgatcgaa ggcgcgattc
     1801 tcgcgctggc cgagggcgac ctggagcggg ccaagagggt acagaacggc gatgacgaga
     1861 tcgacgatct gttcatcgac atggagaagc gcgccatggc gatgttggcg caacagcaac
     1921 cggttgcggg cgacctgcgg ctcatcgtga ccatcctgcg ggtggtgaac gacctcgaac
     1981 gcgcgggcga cctggctttc aacatcgcgt cggttgtgcg caaggacctg gatatcgtca
     2041 agctgcgcaa ggtcagcgcc ctcgtctacg agctcggcca cgcttcccgg cggcttctgt
     2101 ccaaggcgct cgacgtctgg tctgagaaga acctggaact cgccggccag atggacgact
     2161 tggacgacga gatcgacgaa ctctacgagc gcctgttccg cgagctgttc gagctgcaga
     2221 acgagaccac cttcgacgta gcgatgaacc tggtactcgt ggggcgttac ttcgagcgca
     2281 tcgccgacca cgcggtgaac ctcgccgaac gcactcgcta cttcatcacc gggaacgagg
     2341 agcatttggg ttgatgcggc cgccgcgcgg tcgacggcgc cggggtgcgg gggcgcggga
     2401 gcgcgggagc gccgcgccgt cgctgagccg ccgtgccgac ggcagaga
//
LOCUS       DASRMJ010000079         6720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_5706, whole genome shotgun sequence.
ACCESSION   DASRMJ010000079 DASRMJ010000000
VERSION     DASRMJ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5706"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFD74_02300"
     CDS             complement(<1..494)
                     /locus_tag="VFD74_02300"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZK48418.1"
                     /translation="MTERGHTDHDVLTTSFGKAVDDDQNSLTAGEAGPILMQDVHLLD
                     KLAHFDRERIPERVVHAKGAGAYGYFEVTVDVTKYTKAAFLSEIGKRTDVFLRFSTVG
                     GEKGSADAERDPRGFALKFYTEEGNYDMTGNNTPVFFTRDPLKLPARRTPRRVPALPW
                     SRSAS"
     gene            complement(637..2262)
                     /gene="hcp"
                     /locus_tag="VFD74_02305"
                     /gene_synonym="priS"
     CDS             complement(637..2262)
                     /gene="hcp"
                     /locus_tag="VFD74_02305"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HZK48419.1"
                     /translation="MFCNQCEQTRKGSGCTTAGVCGKPPEVAALQDLLTYALRGLSQV
                     AIAARDAGVSDAKVDSFTSRALFSTLTNVNFDASRFQTLILEAVFRRDQLEEQLVAAG
                     GAVPTHDPAIAFHPAPDMVGLVRQGAKVRLPIDWEADEDIRSLQEITLYGLRGLAAYA
                     DHAAILGQEDPDVYAFVQSTLTKLADRSLGLNDWVGLALETGKVNLRAMELLDAGNTG
                     TYGHPVPTSVPLGHKQGKAILVSGHDLRDLHMILEQSAGKGINVYTHGEMLPTHGYPG
                     LKKYPHFFGHYGTAWQNQKGEFGEFPGAILMTTNCLQDPKEYMDNIFTTGLVGWSGVR
                     HLENGDFGPVIEKALEMPGFAKDQTRDSVTVGFARNAVMGVAGKVIEAVKSGDIKHFL
                     LVGGCDGAKPGRNYYTELVERAPADTVILTLACGKFRFFDRKLGDIGGIPRLLDIGQC
                     NDAYSAVQIALALADAFEVGVNDLPLSIVLSWYEQKAVSILLTLLHLGIKGIRIGPSL
                     PAFISENVLNVLVENFDLKPVGDPDEDLAAMLA"
     gene            complement(2379..2651)
                     /locus_tag="VFD74_02310"
     CDS             complement(2379..2651)
                     /locus_tag="VFD74_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48420.1"
                     /translation="MRSETHAASDTQRESTVTGGGVMNPKLAWEELKAKMADNRRAVE
                     HVHLVGEAHRACQACGSENSEHAVACAVCGARLQPKDSSAVGNDVP"
     gene            complement(2730..2972)
                     /locus_tag="VFD74_02315"
     CDS             complement(2730..2972)
                     /locus_tag="VFD74_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZK48421.1"
                     /translation="MQVTIDVCAVLRGCCRADGRGFEGGPTEIAENDTLGDVLGRLSL
                     PEQLKWIACVNGRPSPPDTSLSEGDHLYVFLPVSGG"
     gene            complement(3061..4893)
                     /locus_tag="VFD74_02320"
     CDS             complement(3061..4893)
                     /locus_tag="VFD74_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZK48422.1"
                     /translation="MNGWTGTILRVDLTAGTVAEETLDPQVARDYVGGRGLGIYYLNR
                     ELDPMVEPLSPENMVCMFTGPLTGTRAATGARYMIMTKSPLTGALTTSNSGGHFPTEL
                     KRAGYDGIILTGRAAEPTYLFVTPGKAELRSADHLWGKDTEETTDLLLQGTHPKARVA
                     CIGPAGERLVRFASVQNEKHRAAGRSGVGAVLGSKNLKAVVVKAEAKDVPLHDPEAFA
                     AYHKEVIDAYDAATAVTPSGLTLYGTTYVVGHSEKMGCLPTKNFQRTKFTPWEGGIEK
                     EFHEKHFIRRGACFSCPIGCARISKVEKPDRTLTGEGPEYETWYAMGSSCEISDLASI
                     VEANFLCNRLGLDTITMGATVACAMELVDRGYLDEAEVGRSLRWGDGGALVELTEKTG
                     NREGFGDLLAEGSYRLAERCGHPELAMVSKKQEFAGYLPRRLQGMSLTYATSPIGGSH
                     MRGDTAYFELFGTPVLVDSAAVEGKAELAAACQDLSTIIDSSGLCIFFAHRALISKDA
                     KARPVGIMEYLNAATGADYTIEELCEAGERIANAERRFLTRAGFSRKDDTLPPRELEE
                     PLPDGPAAGQVSHLPEMLETYYQVRGWDTDGIPTPATLGRLGIA"
     gene            5142..>6720
                     /locus_tag="VFD74_02325"
     CDS             5142..>6720
                     /locus_tag="VFD74_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HZK48423.1"
                     /translation="MASVSRVAARRARRSDHVDDPTGLSPAEAGERLRSEGYNELPSS
                     EGRGVSAIAVGVIKEPMFLLLVFSGALYATLGDIQEAAMLLGFVFVVMGITVYQERKT
                     ERALDALRDLSSPRACVVRGGRQLRIPGREVVRGDIVMVSEGDRVPADAAVLSCNDLQ
                     VDESLLTGESVPVRKLPAEGPFDSETILVRPGGDDLPNIYSGTLVVQGQGIAEVGATG
                     TRTEMGRIGLALEQTDREPTDLQRQSGRLVRNMALVGVFVCCLVVLMYGWTRGDWIDG
                     LLAGVTLAMATLPEEIPVVLTVFLALGAWRISQKNVLTRRIPAVESLGSATVLCVDKT
                     GTLTVNRMTIARLFAAGRFFVVPEERAGAAHAEAAAAETGGMEHHAAAAGRHVAAGDV
                     GDAAAGGGPDAVAVAAIPAAASPGIAAATAPGVPSGSGAAGPPLGAPVHEAFHRLVEY
                     GILASELDPFDPMEKAFDELGGRYFAASEHLHRDWELVHEYSLSRDLLAMSHVWRSPT
                     RDACVVAAKGAPHVRHRE"
BASE COUNT         1006 a         2173 c         2333 g         1208 t
ORIGIN      
        1 gaggcagatc gtgaccaggg caacgccggc acccggcgcg gcgttcgacg cgctggcaac
       61 ttcagcggat ctcgggtgaa gaagaccggc gtgttgttgc cggtcatgtc gtagttcccc
      121 tcctccgtgt agaacttgag ggcgaaaccg cgcggatcac gctcggcgtc ggcggagccc
      181 ttctcgcccc ccacggtgga gaagcgcaga aagacgtcgg tgcgcttgcc gatctcggag
      241 aggaaggcgg ccttcgtgta cttggtcacg tctactgtca cctcgaagta gccgtaggcg
      301 cccgcgccct tggcgtgcac cacccgttcg gggatgcgct cgcggtcgaa gtgtgcgagt
      361 ttgtcgagca ggtggacgtc ctgcatcagg atggggcccg cctcgccggc ggtcaggctg
      421 ttctggtcgt catccacagc cttgccgaag ctggtggtga ggacgtcgtg gtctgtgtgt
      481 ccgcgctcgg tcatcggtgg cctcctggtg ttatcgatcc gtctgcagcc ttcccggtga
      541 gaaggacgcg tacacacgag aaaagccccg gccctcggtc gagaccgagg gccggggctt
      601 gtaggatggc gcgagaggcc cgtctgcagc gggttcctac gccagcatgg ccgcgagatc
      661 ctcgtcgggg tcgcccaccg gcttgaggtc gaagttctca acgagcacgt tcagcacgtt
      721 ctccgagatg aaggcgggca ggctcgggcc gatgcggatg cccttgatac ccaggtgcag
      781 cagcgtgagc aggatagaga ccgccttctg ctcgtaccag gagagcacaa tagacagtgg
      841 caggtcgttg acgcccactt cgaaggcgtc ggcgagagcc agggctatct ggacagccga
      901 gtaggcgtcg ttgcattgac ctatgtcgag caggcgcggg atgccaccga tgtcgcctag
      961 cttgcggtcg aagaagcgga acttaccgca ggccagggtg aggatgacag tgtcggccgg
     1021 ggcgcgctcc accagctcgg tgtagtagtt gcggcctggc ttggcgccgt cgcagccgcc
     1081 caccaacagg aagtgcttga tgtcgcccga cttgaccgcc tcgatcacct tcccagccac
     1141 tcccatgact gcgttgcgcg cgaagcccac cgtcacgctg tcgcgggtct ggtctttggc
     1201 gaagcccggc atctccagcg ccttctcgat caccggcccg aagtcgccgt tctccaggtg
     1261 gcggactccc gaccagccca cgagcccggt ggtgaagatg ttgtccatgt actccttggg
     1321 gtcctgcaga cagttcgtgg tcatcaggat ggcgcccggg aactcgccga actcaccctt
     1381 ctggttctgc caggccgtgc cgtagtgacc gaagaagtga gggtacttct tcagccccgg
     1441 gtagccgtgg gtgggcagca tctcgccgtg cgtgtagacg ttgatcccct tgcccgccga
     1501 ctgctcgagg atcatgtgca ggtcgcgcag gtcgtgcccc gacaccagga tggccttgcc
     1561 ctgcttgtgg ccaagaggca cgctcgtggg caccgggtgg ccgtaggtgc cggtgttgcc
     1621 cgcgtccaac agctccatgg cccggaggtt gaccttgccg gtctcgagcg ccaggccgac
     1681 ccagtcgttc aggccgagcg acctgtcggc gagcttggtc agggtcgact gcacgaaggc
     1741 atagacgtcc ggatcctctt ggcccaggat ggcggcgtgg tcggcgtagg cggcgagccc
     1801 gcgcaagccg tagagggtga tctcttggag actacggatg tcttcgtcgg cctcccagtc
     1861 gatgggcagc cggactttgg cgccctggcg caccagcccc accatgtccg gggcggggtg
     1921 gaaggcgatc gctgggtcgt gcgtgggcac cgcgccgccg gctgcgacca gctgctcctc
     1981 gagttggtcg cggcgaaaga cagcttcgag tatcagcgtc tggaagcgtg aggcgtcgaa
     2041 gttgacgttc gtaagcgtgg agaagagggc ccgcgaggtg aagctgtcga ccttggcgtc
     2101 actgacgccc gcgtctcggg cggcgatcgc cacctgggag aggcctctca gcgcgtaggt
     2161 caggaggtct tgcagcgccg cgacctccgg cggcttccca cagactcccg ctgtcgtgca
     2221 gccgctccct ttgcgtgtct gctcgcactg attgcagaac atcccgagat tctccacccg
     2281 cgcacctccg ttggctcatt cgaccggtag cagtgctcgc ttatcagcgt tagcacggtg
     2341 caaacggtgg gaaccatacc gcagaggctg ggtgggagtc aagggacgtc gttgccgacg
     2401 gccgaagagt ccttcggctg cagcctggct ccgcacacgg cgcaggcgac cgcgtgctcg
     2461 ctattctcgg atccgcaggc ttggcaggcc cggtgtgcct cgccgacgag atgcacgtgc
     2521 tcgacagccc gtcggttgtc cgccatcttc gctttgagct cttcccacgc cagcttcggg
     2581 ttcatgacgc cacccccggt gaccgtcgac tctctctgtg tgtccgaggc cgcgtgggtc
     2641 tcggagcgca cctttcacta ctatgtacca tagtaaggag gaatgtaggc ctcggaagtg
     2701 ggcttcgaag tcctgggagc ggtccgccct cagccccccg acaccgggag gaagacgtag
     2761 aggtggtcac cttccgacag cgaggtgtcc gggggcgacg gtctaccgtt gacgcaggcg
     2821 atccacttca gctgctcggg taggctcaga cggccgagga cgtcgcccag cgtgtcgttc
     2881 tcggctatct cggtcggccc gccctcgaag ccgcggccgt cggctcggca gcagccccgc
     2941 agcacggcac acacgtcgat ggtcacctgc atctcgtctc ctggctccgc ggcggcgcgt
     3001 tcggcggacc gccggccggg atccggacgc ctccgtcggg gccgtccgcg gccggcgcgg
     3061 ctaggcgatc cctagccttc ccagtgttgc cggcgtgggg atgccgtcgg tatcccagcc
     3121 ccgcacctgg tagtaggtct cgagcatctc gggaaggtgg gacacctgcc cggccgcggg
     3181 gccgtccggg agtggctcct cgagttcgcg tggcgggagg gtgtcgtcct ttcgcgagaa
     3241 gccggcccgg gtcaggaatc ggcgctcggc attggcgatg cgttccccgg cttcgcacag
     3301 ctcttcgatg gtgtagtcgg cgcccgtggc cgcgttcagg tactccatga tgcccaccgg
     3361 ccgggccttg gcgtccttgc tgatgagggc ccggtgggcg aagaagatgc agagacctga
     3421 ggagtcgatg atggtggaga ggtcctggca ggcggcggcc aactcggcct tcccctcgac
     3481 cgcggcgctg tcgaccaaga cgggcgtgcc aaagagttcg aagtaggcgg tgtcgccgcg
     3541 catgtgcgag cccccgatgg gcgacgtggc gtaggtcagg ctcatcccct ggaggcggcg
     3601 ggggagatag cccgcgaact cctgtttctt cgagaccatc gccagctccg gatgaccgca
     3661 tctctccgcc aagcggtaac tgccctcggc gagcaggtcg ccgaagcctt cgcggttgcc
     3721 cgtcttctcg gttagttcca ccaacgcgcc gccgtcgccc cagcgcagcg accgtcccac
     3781 ctccgcttcg tcgaggtagc cgcggtccac caactccatg gcgcaggcga ccgtggcgcc
     3841 catggtgatc gtgtccagcc ccagcctgtt gcagaggaag ttcgcttcga cgatggacgc
     3901 caagtcgctg atctcgcagc tggaccccat cgcgtaccag gtctcatact cggggccctc
     3961 gccggtgagc gtccgatcgg gcttctccac cttgctgatg cgggcgcacc cgatggggca
     4021 gctgaagcag gcgcctctgc ggatgaagtg cttctcgtgg aactccttct cgatgccgcc
     4081 ttcccacggc gtgaacttcg tcctctggaa gttcttggtg ggtaggcagc ccatcttctc
     4141 gctgtggccc acgacgtagg tggtgccgta gagggtgagt cccgagggcg tcacggcagt
     4201 cgcggcatcg taggcgtcga tcacctcttt gtggtaggcc gcgaacgctt ccggatcgtg
     4261 gagagggacg tccttggctt ctgctttgac cacgacggcc ttcaggttct tcgaacccag
     4321 cacggcgccc acgcccgagc gccccgcggc gcgatgcttc tcgttctgca cggaggcgaa
     4381 ccgcaccagc cgctcccccg ccgggccgat acaggcgacg cgggccttgg ggtgggtccc
     4441 ctggagcagg aggtcggtgg tctcctcggt gtctttgccc caaaggtggt cggcggagcg
     4501 gagttcggcc tttccgggag tcacgaagag gtaggtgggc tccgcggcgc gccccgtgag
     4561 gatgatgccg tcatagccgg cacgcttgag ttcggtgggg aagtggccgc cggagttgga
     4621 cgtggtgagc gccccggtga ggggactctt ggtcatgatc atgtagcggg cgccggtggc
     4681 ggcccgcgtc ccggtgagcg gcccggtgaa catgcacacc atgttctcgg gggagagggg
     4741 ttcgaccatg gggtcgagtt cccggttcag atagtagatg ccgaggccgc ggcccccgac
     4801 gtagtcgcgc gcgacctgcg ggtcgagagt ctcttcagcg acggtacccg cagtcaagtc
     4861 gacgcgcagg atggtgccgg tccaaccgtt cattccacaa cctcaccgga caggattgac
     4921 tcatgcccac agcctaccac cggcgcgtcg tcgtcgggat tccttggggt tcgcatgtcg
     4981 tgccaaatct tggttcccgg gaggcctgtg cgcggcgctg gacgcgggag aaaccgtgct
     5041 gcgctggatg cgggagaggc cgtgcggcgc agccgtggtt tgcgacagtg ggttgcgaca
     5101 gtgggttgtg gacgctcccc cgtgggtagc gtggccagcg gatggcgtcg gtatctcgcg
     5161 tcgcggcacg acgagcacgg aggtctgacc acgtggatga tccaaccggc ctctctccgg
     5221 ccgaggcggg tgagaggctc cgctccgaag gctacaatga gctgccctcg agcgaaggtc
     5281 gaggcgtctc ggccatagcg gtaggcgtga tcaaggagcc gatgttcctg ctcctggtct
     5341 tctcgggcgc cctctatgcc acgctcggcg acatccagga ggcggcgatg ctcctgggct
     5401 tcgtgttcgt ggtcatgggc atcaccgtct accaggagcg caagaccgag cgtgccctcg
     5461 acgcgctgcg cgatctgtcg agtccgcggg cctgcgtcgt caggggaggg cggcaactcc
     5521 gcatccccgg ccgcgaggtc gtgcggggag atatcgtgat ggtctctgag ggcgatcgcg
     5581 ttccggccga cgcggccgtg ctctcgtgca acgatctgca ggtcgacgag tcgctgctca
     5641 ccggcgagtc ggttcccgtg cgcaagctgc ccgccgaggg cccttttgat tccgagacaa
     5701 ttctggtgcg accgggcgga gacgacctgc cgaacatcta ctcaggcact ctcgtggtgc
     5761 agggccaggg catcgcggaa gtgggggcga caggaacgcg caccgagatg gggaggatcg
     5821 gccttgccct cgagcagacc gacagggagc ccaccgacct gcaacgtcaa agcggccgcc
     5881 tggtgcgcaa catggccctg gtgggggtct tcgtctgctg ccttgtcgtg ctcatgtacg
     5941 gttggacgcg gggagattgg atagatgggc tcctggctgg ggtgaccctc gccatggcta
     6001 cgctgcccga ggagatcccg gtcgtgctca cggtcttcct ggctctcggg gcgtggcgga
     6061 tcagccagaa gaacgtcttg acccgcagga tcccggccgt cgagtcgctg ggatccgcca
     6121 ccgtgctgtg cgtggacaag accggcaccc tgacggtcaa tcgcatgacc atagcccgac
     6181 tcttcgcggc gggtcggttc ttcgtcgtgc cggaggaacg ggcgggggcc gcccacgcgg
     6241 aggccgccgc cgcagagacc ggcggcatgg agcaccacgc tgcggcggct ggtcgccacg
     6301 tcgcagcggg ggacgtcggc gatgcagccg ccggtggcgg ccccgacgcc gtggcggtcg
     6361 ccgccatccc cgcggccgcc agcccaggca tcgcagccgc caccgctccc ggcgtcccct
     6421 ccggctctgg cgccgccggg cctccgctgg gcgctccggt gcacgaggcc ttccacagat
     6481 tggtggagta cgggatcctc gccagcgagc tcgacccctt cgaccccatg gagaaggcgt
     6541 tcgacgaact cggggggcgc tacttcgccg catccgaaca cctccaccgc gactgggagt
     6601 tggtgcacga atactcactg tcgcgcgact tgctcgcgat gtctcacgtg tggcgctcac
     6661 ccactcgcga cgcgtgcgtc gtcgccgcga agggagcgcc acacgtgaga catcgcgagc
//
LOCUS       DASRMJ010000080         5155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_408341, whole genome shotgun sequence.
ACCESSION   DASRMJ010000080 DASRMJ010000000
VERSION     DASRMJ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408341"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="VFD74_02330"
     CDS             <1..1471
                     /locus_tag="VFD74_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK48424.1"
                     /translation="NQPCIIFMDEIDAVGRHRGAGMGGGHDEREQTLNQLLVEMDGFE
                     ANESIIMIAATNRPDILDPALLRPGRFDRQIVVDRPDREGRRAILEVHSKGKPLSPDI
                     DLGVLAASTPGFTGADLANLVNEAALLAARQNKKVVEMDELEEAILRVIAGPEKKSRL
                     LSEEERLITAYHETGHALVAHLLPNADPVHKVSIISRGQALGYTITLPTEDKFMVKRG
                     EIMDKLAHMLGGRVAEEVRFDDITTGASNDLQRATETARRMITQYGMSENLGPLTFGH
                     DPAQPFVGRDYGMGQEYSEATSQRIDNEIRRVIDEAYSTANRILREHRAELDMVSQLL
                     VERETIDRVEFEALLAGGDPAEVFKERDEKNALKAAEKKKRDEQSRARDIAKARDRDE
                     RAADDDDTDGLPAPAAAEVVDVDDAVEVPVADIDAADIKAAEAAEAPAAPQALEVGES
                     DTPVDDLSSPDGAGGKSAAPAGDDSQVEADAPGDDGEPK"
     gene            1522..2091
                     /gene="folE"
                     /locus_tag="VFD74_02335"
     CDS             1522..2091
                     /gene="folE"
                     /locus_tag="VFD74_02335"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZK48425.1"
                     /translation="MDLDMIEEGIRLVLLGLGEDAARDGLARTPRRVAEMYQEVLSGT
                     YQNLADIVQPIPSDKHQELVLIKDIPFHSVCEHHLTPFFGFAHVAYIPSRDGMIAGLS
                     KLARLVKVAAARLQLQERLTSCIADTLVETLDPEGVLVLVQAEHLCMSMRGVRTAGAQ
                     TVTSAVRGIFRTSSATRAEVLALIDAPRK"
     gene            2096..3310
                     /gene="folP"
                     /locus_tag="VFD74_02340"
     CDS             2096..3310
                     /gene="folP"
                     /locus_tag="VFD74_02340"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZK48426.1"
                     /translation="MIPRAGLPQVVALRDLDEVRALMARMRVSPGGANIMDKKALFRV
                     VRVKGLDVRAANILKQEMLSRGGEVATSREVYELNGREADCLVFGTLTQFERLLPKLR
                     AQPFGLRDLAASIAAALEHYDARVPAFHAGFDLSKRPRVMGIVNVTPDSFSDPGDHFD
                     PDVAVAAAWRMVEEGADMIDVGGESTRPGSGRVSADEEIARVLPVIKALAGSLPVPIS
                     IDTYRASVAAAALEAGAFMVNDVSAFRMEPAIADVVRDAGCPAVLMHMLGEPRTMQDN
                     PVYDEVVQDLFTFFVERLTWAVERGVPEENLLVDPGIGFGKTLVHNLEIMRHIGSFRS
                     LGRPIVLGASRKRFIGEILGLPEAKDRVIGTVATSVIAALHDVDIVRVHDVRENVEAI
                     RLARAVFPTPEA"
     gene            3343..3732
                     /gene="folB"
                     /locus_tag="VFD74_02345"
     CDS             3343..3732
                     /gene="folB"
                     /locus_tag="VFD74_02345"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZK48427.1"
                     /translation="MDIYIDGLEVYGYHGVLPEEKVLGQQFVFDLRLSIDECVGVESD
                     RVADTIDYTEVIDVVTEVAGCESYDLLERVAGSIARTLLGRFPIDAVWVRVTKPHPPI
                     PCALRGVGVSLELRRQGGALGAGGSIA"
     gene            3738..4262
                     /gene="folK"
                     /locus_tag="VFD74_02350"
     CDS             3738..4262
                     /gene="folK"
                     /locus_tag="VFD74_02350"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZK48428.1"
                     /translation="MSQSLDAPGAGVTAYLGLGSNQGDSRTLLEEAVRGLSTVAGVRV
                     TGLSRCYRTAAVGMEDQPDFLNMIVEIATTLAPLELLEAVQSLESAAGRVRTVRWGPR
                     TLDVDILWYDGIFMDHDRLQLPHPRMQERRFVLEPLAELAPDLVLGNGLTVRESLAEV
                     LDQDVAVMVDPRKE"
     gene            4272..>5155
                     /gene="nadA"
                     /locus_tag="VFD74_02355"
     CDS             4272..>5155
                     /gene="nadA"
                     /locus_tag="VFD74_02355"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZK48429.1"
                     /translation="MDYSAMQEELKTLARDRDAVILAHNYQRPEVQDAADYVGDSLGL
                     SRQAAASDAGIIIFCGVDFMAETAAILAPDKRVILPEERAHCPMAAMVDERGLAALKA
                     QHPGAVVVSYVNTSAAVKALSDICCTSANAPEVVASVPAGTEIIFTPDCNLGAWVTEK
                     TGREMILWQGFCPTHDLIQPEDILEARALHPKAKVVVHPECRPDVTALADAVESTSGM
                     IRFCREDEAREFIVGTESGMIHRLSVDVPGKRFHLVNDSVVCPNMKMTTLGKAIAALR
                     REGPVVTVPEEIRLKALAA"
BASE COUNT          909 a         1686 c         1733 g          827 t
ORIGIN      
        1 gaatcaaccc tgcatcatct tcatggacga gatcgacgcc gtcggtcggc accgcggcgc
       61 cggcatgggc gggggccacg acgaacgcga gcagaccctg aaccagctgc tcgtggagat
      121 ggacggtttc gaggccaacg aaagcatcat catgatcgcg gccaccaacc gtcccgacat
      181 cctcgacccc gctctgctgc ggcccggtcg tttcgatcgc cagatcgtgg tcgatcgtcc
      241 cgatcgcgag ggccgccggg ccatcctgga ggtgcacagc aagggcaagc cactctcgcc
      301 cgacatcgac ctgggcgtgc tggccgcgtc tactccaggg ttcaccggcg ccgatctggc
      361 caatctggtg aacgaggccg cgctgcttgc ggcccgccag aacaagaagg tcgttgagat
      421 ggacgaactc gaagaagcca tcttgcgcgt catcgccggg ccggagaaga agtcccgcct
      481 cctcagcgag gaagagcgtc tcatcaccgc atatcacgag accgggcacg cgctggtggc
      541 gcacctgctg ccgaacgcag acccggtgca caaggtatcc atcatcagcc gcggccaggc
      601 tctcggctac accatcaccc tgcccaccga ggacaagttc atggtgaagc gcggcgagat
      661 catggacaag ctcgcacaca tgttgggcgg gcgggtggcc gaggaggtgc gcttcgacga
      721 catcaccaca ggcgcttcca acgacctcca gcgggccacc gagacggccc gccgtatgat
      781 cacccagtac ggcatgagcg agaacctggg gccgctcacg ttcgggcatg accccgcgca
      841 gcctttcgtc ggacgcgact acggcatggg gcaggagtac tcggaagcga cctctcagcg
      901 catcgacaac gagatacgcc gggtcattga tgaggcctac agcaccgcca accgcattct
      961 tcgcgagcat cgcgcggagc tggacatggt gtcgcagctg ctggtggagc gcgagaccat
     1021 cgatcgcgtg gagttcgaag cgcttctcgc cggcggggat cctgccgaag tgttcaagga
     1081 acgtgacgag aagaacgcct tgaaagcggc tgagaagaag aagcgcgacg agcaatcccg
     1141 cgcccgcgac atcgcgaagg ctcgcgaccg cgatgagcgc gcggccgacg acgacgacac
     1201 cgatggttta ccggcgccgg ctgccgctga ggtggtcgat gtcgacgatg ccgtcgaagt
     1261 gccggtcgcc gacatcgatg cggccgatat caaggccgct gaggccgccg aagcgcccgc
     1321 ggcgccccaa gcgctcgagg tcggcgagag cgacacaccg gtcgacgacc tgtcgtctcc
     1381 agacggcgcg ggcggcaagt ccgcggcgcc ggccggggac gacagccagg tggaagccga
     1441 cgcaccgggc gacgacgggg agccgaagtg agcagggacg aggacccgcg ctcgaaaggg
     1501 cgggcggtgc cgcggccggg catggacctc gacatgatcg aagagggcat ccggctggtg
     1561 ctcctgggac tcggcgagga cgccgctcgc gacggcctcg cacgcacgcc ccgtcgcgtg
     1621 gctgagatgt accaggaagt actgagcggc acctaccaga atcttgccga catagtgcag
     1681 ccgatcccgt cggacaagca tcaagagctg gtgctgatca aggacatacc gttccactcg
     1741 gtctgcgagc atcacctcac cccgttcttc gggttcgccc acgtggcgta catcccgtcg
     1801 cgagacggga tgatcgcggg gctctccaaa ctggcgcgac ttgtcaaggt cgcggcggcg
     1861 cgcctgcagc tccaagaacg actcacgagc tgcatcgccg acaccttggt cgagacgctc
     1921 gatccggaag gcgtcctggt cctggtgcag gccgaacatc tctgcatgtc catgcgcggg
     1981 gtgcgcaccg cgggtgctca gaccgtcacc tccgccgtgc gcggcatctt ccgcaccagc
     2041 tccgcgacgc gggccgaggt gctggcactg atcgacgccc cccggaagta gcccgatgat
     2101 ccctcgagcc ggccttcccc aggttgtcgc tcttcgcgac ctggacgaag tgcgtgctct
     2161 catggcgcgc atgcgggtgt cgccgggcgg cgccaacatc atggataaga aggccctctt
     2221 tcgggtcgta cgggtcaaag gcctcgatgt ccgcgccgcc aacatcctga agcaggagat
     2281 gctctcccgc ggcggcgagg tggccacctc gcgagaggtg tacgagctga acggtcgcga
     2341 agccgactgt ctggtcttcg gcactctcac ccagttcgag cgtctcttgc cgaagttgcg
     2401 ggcccaaccc ttcgggttgc gcgatctcgc cgcctctatc gccgccgccc tcgagcacta
     2461 cgacgcccgt gtcccggcgt tccatgcggg cttcgacctc agtaagcgcc ctcgcgtgat
     2521 gggcatagtg aacgtgactc ccgactcgtt ctccgatccc ggtgatcact tcgacccgga
     2581 cgtggccgtg gccgccgcgt ggcgcatggt cgaggaaggg gccgacatga tcgatgtggg
     2641 aggggagtcc acgcgccccg gctcgggcag ggtcagcgcc gatgaagaga tcgcgcgggt
     2701 gctgccggtg atcaaggctc tggcgggatc cctgcccgtg cccatctcga tcgacacgta
     2761 tcgagcttct gtcgccgcgg cggctctgga ggcgggcgcg ttcatggtga acgacgtgtc
     2821 ggcattccgc atggaaccgg cgatcgccga cgtcgtccga gacgccggct gcccggccgt
     2881 tctcatgcac atgctgggcg agccgaggac gatgcaggac aacccggtct atgacgaagt
     2941 ggtccaggac ctgttcacct tcttcgtgga gcgcctcacc tgggccgtgg agcggggggt
     3001 cccggaagag aaccttctcg tcgacccggg catcgggttc ggcaagacgt tggtgcacaa
     3061 cctcgagatc atgagacata tcggtagctt ccgctccttg ggccgcccta tcgtgctcgg
     3121 tgcttcgcgc aaacgcttca tcggcgagat cctggggctg cccgaggcca aggaccgggt
     3181 gatcggcacc gtggccacca gcgtgatcgc ggccctgcac gacgtggaca tcgtgcgagt
     3241 gcacgacgtg cgtgagaacg tcgaggctat ccgcttggct cgagcggtgt tcccgacgcc
     3301 cgaagcgtag tcggttcggg caccgtcaga gaggggggtc acgtggacat ctacatcgac
     3361 ggactcgagg tctacggcta ccacggcgtg ctccccgagg agaaggtcct cggccagcag
     3421 ttcgtcttcg acctgcgcct ctccatcgac gagtgtgtcg gcgtcgagtc cgaccgcgtc
     3481 gcagacacga tcgactacac tgaggtgata gacgtggtca ccgaggtggc cggctgcgag
     3541 agctatgatc tcctggagcg cgtcgcgggc tccatcgccc ggaccctgct cggccgcttc
     3601 cctatcgacg cagtatgggt gcgagtcacc aaaccccatc cgcccatccc gtgcgctctg
     3661 agaggcgtgg gcgtgtcact cgaactccgt cgtcagggtg gcgcgctcgg ggccggcggg
     3721 tcgatcgcgt agacccggtg tcgcaaagtc tggatgctcc tggagccggc gtcaccgcct
     3781 acctcggtct cgggtcgaac cagggggact cccggactct tctcgaggag gcggtgcgcg
     3841 gtctctcgac cgtagcgggt gtgcgggtga cggggctctc ccgctgctat cgcacggcgg
     3901 cggtgggcat ggaggaccaa ccggatttcc tgaacatgat cgtcgagatc gccacgaccc
     3961 tggccccact cgaactgctg gaagccgtcc agagcctgga gagcgcggcc ggaagggtgc
     4021 gcaccgtccg ctggggcccc cggacgctcg acgtggatat cctctggtac gatggcatct
     4081 tcatggacca cgaccgcctc caactgccgc atcctcggat gcaggagcgg cgcttcgtgt
     4141 tggagcccct ggcggaactc gcccccgacc tggtgttggg caatggcctc acagtgaggg
     4201 aatccctggc cgaggtgctc gatcaggacg tggcggtaat ggtcgatcca cggaaggagt
     4261 gaggcggagt catggactac tcggccatgc aagaagaact gaagaccctt gctcgcgaca
     4321 gagacgcggt gatactggcg cacaactacc agcgacccga ggtgcaggac gccgcggact
     4381 acgtgggcga ctcgctgggc ctctccaggc aggcggccgc cagcgacgcg ggcatcatca
     4441 tcttctgcgg tgtggacttc atggccgaga ccgcggccat cctggcgccc gacaagcggg
     4501 tcatactccc tgaggagagg gcgcactgcc ccatggcggc catggtcgac gagcgggggt
     4561 tggccgcgct gaaggctcag caccccggcg cggtggtggt gagctacgtg aacacgtcgg
     4621 cggcggtgaa ggcgctttcg gacatctgct gcacgagcgc caacgcgccc gaggtcgtgg
     4681 cctccgtgcc cgccggcacg gagatcatct tcacccccga ctgcaatctg ggcgcctggg
     4741 tgaccgagaa gacgggccgc gagatgatcc tgtggcaggg cttctgcccc acccatgacc
     4801 tcatccagcc cgaagacatc ctcgaggcca gagcgctgca tccgaaggcc aaggtggtgg
     4861 tgcaccccga atgtcgcccg gacgtcacag ccctggccga tgccgtggag agcacgtcgg
     4921 ggatgatccg attctgccga gaggacgaag ctcgggagtt catcgtaggc accgagtcgg
     4981 gcatgatcca ccggttgagc gtggatgtcc ccggcaagcg cttccacctt gtcaacgact
     5041 cggtcgtgtg tccgaacatg aagatgacca ctctcggcaa ggcgatcgcg gccctgcgac
     5101 gtgaaggtcc cgtggtcacc gtccccgagg agatccgact aaaggcgttg gcggc
//
LOCUS       DASRMJ010000081        11031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_511856, whole genome shotgun sequence.
ACCESSION   DASRMJ010000081 DASRMJ010000000
VERSION     DASRMJ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11031
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511856"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFD74_02360"
     CDS             complement(<1..504)
                     /locus_tag="VFD74_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZK48430.1"
                     /translation="MTSDLTSELAGIPGSRSWRRAPLAPFTTIGVGGNADLLVTVDTA
                     DALIAAVRLLSDADVPWVVLGGGSNFLVPDSGYRGVVLKLDHHFHYVEEPEDADASSA
                     WLVAGAGLPLSRLAVHVADLGLSGLEFACGIPGSVGGGVCMNAGAHSGCMADVVDAVH
                     IVSGEGLG"
     gene            complement(524..1924)
                     /gene="murC"
                     /locus_tag="VFD74_02365"
     CDS             complement(524..1924)
                     /gene="murC"
                     /locus_tag="VFD74_02365"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZK48431.1"
                     /translation="MTNSTLFQPDLRLSGLRRLHFIGIGGAGMSGIALVLHRRGYEVT
                     GSDLKSSRYTGLLEEAGVTVTLGHDPRNLDHPDVVVISSAIPEHNRELSAARRRGFPV
                     VRRAQALAWVMEGRRGVAVCGTHGKTTTTSMISRTLMDVGLDPSFLVGGELNDLGSNA
                     RHGAGEIMVCEADESDGSLLYLRPEVAVFTNLELDHHSHYRHVDDVADVFRRFIAQLP
                     EHGRMVYWADDAKLAKLATDARCRTTSYGLGAGADYQAREVGLHRRACSFEVWRHGEH
                     LADAELVVPGQHNVLNALACFASLTELQVSPAEIAASLRSFTGAVRRFQWKGERGAVT
                     VVDDYAHHPTELKATLRAARTGDWGRVVAVFQPHLYSRTRYLHQEFAEALLEADVAVI
                     TEIYGAREEPQPGISAKLILDSLLRLSPRFPVVFLPRLTQVVEYLQWSTVPGDLVLTM
                     GAGDVHRVGERFLELS"
     gene            complement(1921..3027)
                     /locus_tag="VFD74_02370"
     CDS             complement(1921..3027)
                     /locus_tag="VFD74_02370"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HZK48432.1"
                     /translation="MSRILIAGGGTAGHVVPALAIADVLTERGYAVHFCGGPHGMESG
                     LVTRAGYPFSSVRIRGFERRLGLTTLRTLASLPVAAVDAWATLRRLRPACVVGVGAYA
                     SGPVVAEAAVARIPSVALEMDAHLGWTNRILSMLVDRVCLSFPIPGRVGGKFVYTGRP
                     VRPSLLTATREQGLERFSLDPALPVVLVFGGSLGARSMNTAAIEAFARGETPFSVIHV
                     TGTADRERVAEALRGRRAVECANPRYQTHAYLDDFELALAAADVAVARAGGSVAELLA
                     RGIPSVLVPFPAATADHQTKNALGLQAEGAALLLPDAELSGERLAAEVARVLEPRRHA
                     GMRERAAALARPDAATRIADEIVQLAGPPRAPTL"
     gene            complement(2960..4321)
                     /gene="ftsW"
                     /locus_tag="VFD74_02375"
     CDS             complement(2960..4321)
                     /gene="ftsW"
                     /locus_tag="VFD74_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZK48433.1"
                     /translation="MNSRNQNRHVAAQQGGDGRSVLRRAETRRQASIREAAAERDVAE
                     DADALGRSAFRLRADQVLIASALGLLAIGLVMMYSVSSAVGFFRTDGDLNFYLRQQSL
                     VAIAGVVCLVVVSRVDYRHWRRLALFGVAVTVVLLLAVHLPGVGRSAGGASRWLDLGP
                     LPLQPSEVAKLAVILVGSHLLASKRAQSGSSKDLVLPFGAIVAVICVLILWQRDLGTI
                     VVVAVATMGLLWVAGMRVRDWIGLAGGGVGLVLLAIFTEDYRRERFLAFLDPFAQAQD
                     SGFQIVQSFLAMGSGGWFGVGPGLSVQKFSYLPEAHTDMILAVLGEELGLVGVGLVIA
                     LFAVFAASGLALARRCAEPFGRYLVAGCTFLIVGQAGINMGGVMGALPLTGVPLPFIS
                     YGRTNLLIVLIAVGIMLSVARFGPVATPIAAHIERREPPNVTHIDSRRRYGGPRGARP
                     GHR"
     gene            complement(4318..5709)
                     /gene="murD"
                     /locus_tag="VFD74_02380"
     CDS             complement(4318..5709)
                     /gene="murD"
                     /locus_tag="VFD74_02380"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZK48434.1"
                     /translation="MGLRADVRRVLVVGAGRSGVAAATLLAGLGREVVLSDRRGLAEL
                     PGIEPALLAGARLLPGPQSPDQLEGVDLLVKSPGVPAEVPLLTAAHHVGIPVWSEAEL
                     AYALLPAPFSAVTGTNGKTTTTALLGHMFKTAGHPALVLGNIGTAITSAARGLGPECE
                     LVVELSSFQLEDVHEFRPAAGVLLNLTPDHLDRHGTMERYLACKANMFARQRSDDWAV
                     FNLDDPAVAGLGREVAAREGGPAVLFFSTLGSPGAAAWFDGVTLHLLGGGIPADSIPL
                     RGRHNMENCLGASTVALARGLPSQAVEEALRTFPGVPHRLQPAGVVDGVAYVNDSKAT
                     NVEATLKALDAYPTGTHLILGGRDKASDYRPVARACAVGCRSVLLIGEAAPLIRAAFE
                     RVRVEEGMETVPVPSDEGDLERAVADAVASATPGDTVLLAPACASFDQYSDFEERGAH
                     FMQIVQRLRADRE"
     gene            complement(5739..6704)
                     /gene="mraY"
                     /locus_tag="VFD74_02385"
     CDS             complement(5739..6704)
                     /gene="mraY"
                     /locus_tag="VFD74_02385"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZK48435.1"
                     /translation="MIRVLAAGLLAMVLAVVAGPRFIRWLRDRGIGQNIRDVGPERHQ
                     VKQGTPTMGGLLILAAAAIPYMILARKTVPSLVVFVLTFGSGAIGLLDDLAKVRRKRS
                     LGISGKTKMSLQLLLVLVVGWVAIRFAGVDSNVHVPLLNKELAFGGVYFLVVYFVISG
                     FANSVNITDGLDGLAAGTVATCLLAYTGITFMLHEPDLAILSASLMGACVGFLWYNSV
                     PAEVFMGDTGSLALGAALAGLAVVTGTELLLVVIGGIFVVEALSVIIQVISFRVFKRR
                     VFLMAPIHHHFELKDWSETKIIVRFWIVTAIFAAAGFTIFYVDTL"
     gene            complement(6701..8209)
                     /gene="murF"
                     /locus_tag="VFD74_02390"
     CDS             complement(6701..8209)
                     /gene="murF"
                     /locus_tag="VFD74_02390"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZK48436.1"
                     /translation="MIELSAEIAARALGEDTVSAPVTGISTDTRTLVKGDLFIALRGD
                     NYDGNQFVPAALAGGAGAAVVELGAAIAGEVDYLPPGGQTLHRVPDTLAALAALAREV
                     RRASTATVVAITGSVGKTSTKDLVSGMAAVVRRVVATSANENNEIGVSLTLLEITPET
                     EVVVVEMGMRGKGQIRSLTLTAEPDVAVITAIAPVHLELIGRMEDVLAAKAEIFEGVR
                     AGGAAVVPVDAFELAAAARLSGVRVVTFDFTDARAASPEIGPDLSEARSLAADVVGVA
                     RRDGNGGALLRLTWPGGEAEVGTAFRAAHRLRNAVAATAACYAAGLPVAECVSGLSSV
                     DFTPLRGDEVLAGGVLIVDDTYNANPVAVRLALDDLAISAESGGRRPIAVLGDMLELG
                     PHADEYHREVGRHAARTGVAALFGVGPRSRHTIQGFRESLRDRLEPLAGMQPGVWSET
                     ADAAVDGIVAYLRPGDVVLVKGSRGMKLDRLVERLVARLEHERCSDEVGGAR"
     gene            complement(8206..9762)
                     /locus_tag="VFD74_02395"
     CDS             complement(8206..9762)
                     /locus_tag="VFD74_02395"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZK48437.1"
                     /translation="MRLADLFREVGGFEVEGDPSTDVSHLSYRSSLAGPGHLFFCVPG
                     FVRDGHDFAPDAVSRGAVALCVERPLDLPVTQVVVPSVRRAMGLVASSLFEHPSARLA
                     TVGITGTNGKTTTGFLCAFFLGETGRRAGLLGTVERRIGGEVFPAERTTPEAVDVQQD
                     LALMVDAGDQAAVMEVSSHALDLERTLGIHFSAVAFTNLTQDHLDYHGTLEAYFASKS
                     RLFLDSDYARNRPPAVINVGDPFGRLLVERLVPDRVLTVAVASDGLTDPGRRTPDLEI
                     RNARLDERGATGSLVLKHRALEAVRRGRGEPVVKGEELSLELSTRLLGRYNLENIAVG
                     LGICLSLGADPQAMLDALPFFPGVPGRLERIDEGQAFTVAVDYAHTPDSVERVLDMAR
                     SIARGRVIGVLGCGGDRDRAKRPLMGRALESRCEVAVLTSDNPRSEDPAAILDDILAG
                     LECPGEAVVEVDRRVAIEAALRRAGAGDLVLILGKGHESGQEFADRVIPFDDREVVRA
                     LVRVIMAESS"
     gene            complement(9812..>11031)
                     /locus_tag="VFD74_02400"
     CDS             complement(9812..>11031)
                     /locus_tag="VFD74_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZK48438.1"
                     /translation="PKGALAPQLLGFVGTDNLGLAGVEKQYDEQLTGVSGKRSIVGDP
                     FGRTLGVLFDEAGRDGRSVVLTIDEDIQFAAEQAVADAVETFGALKGTAIVLDPKTGE
                     ILAMANVPYFDANEFGVQKEADQRNAAVTDQYEPGSTFKVVAVAAALEAGLVQPDTSF
                     DLAPSIKVHDRTVHEAHEDIPAIRSMTVTEILAQSSNVGAVTLGLEVGKERLADMIYG
                     FGFTTRTGIDFPGEASGSMLTPDQWSGSTIANVPMGQGISVTPLQLAVAYGAIANEGV
                     YVQPHLTKGAVTTPSHRVVSAGVARQLRDMLRVTVTDGTGQRAQVNGYVVAGKTGTAQ
                     KVLPNGRGYSNNTFVASFIGMIPADDPALVILVVIDEPTLQYYGSVVAAPAFAEIADF
                     ALRRLEIPPTGQQ"
BASE COUNT         1976 a         3860 c         3554 g         1641 t
ORIGIN      
        1 ccccaggccc tcgccgctca cgatgtggac agcgtcgacc acatcagcca tgcagccgga
       61 gtgcgcgccg gcgttcatgc agaccccgcc gccgacgctc cccggtatcc cgcaggcgaa
      121 ctccagaccc gacaacccca agtcggccac atggaccgcc agtcgcgaga ggggaagccc
      181 cgctccggcc accagccacg ccgacgacgc atcggcgtcc tccggctctt cgacatagtg
      241 gaagtggtgg tcgagcttga ggacgacgcc ccgatacccc gagtccggga cgaggaagtt
      301 cgatcctccc cccaaaacca cccagggaac gtcggcatcg gagagcagtc tcacggcggc
      361 gatgagggcg tcagcggtgt cgacggtcac cagaaggtcg gcattcccgc cgacgccgat
      421 ggtggtgaag ggagccaagg gcgcccgccg ccaagaccgc gagcctggaa tcccggccaa
      481 ctcgctcgtc aggtcggagg tcatccccga ctcggcaaga aggtcacgag agttcgagga
      541 accgctcgcc gactcggtgc acatcccccg cgcccatggt cagcaccagg tcgccgggga
      601 ccgtcgacca ctggaggtac tccactacct gcgtgagcct cggcaagaac accaccggga
      661 accgcggcga caaccgcagc aggctgtcga ggatcagctt cgcactgatg cccggctgcg
      721 gttcttcgcg ggccccgtag atctccgtga tgacggccac gtcggcctcg agcagagcct
      781 cggcgaactc ctggtgcagg tagcgggtgc gcgagtagag atgaggttgg aacacggcca
      841 ccacgcggcc ccagtcgccg gtgcgcgcag ccctcagcgt ggccttgagc tcggtgggat
      901 ggtgcgcata gtcgtccacc accgtcaccg cgccccgttc acccttccac tggaagcggc
      961 gcacggcccc cgtgaagctc cgaagcgaag ccgcgatctc cgccggggac acctgcaact
     1021 cggtcagtga cgcgaaacag gcgagagcgt tgaggacgtt gtgttgaccg ggaaccacga
     1081 gttccgcgtc tgcgaggtgt tcgccgtgcc gccatacttc gaagctgcag gcacgacggt
     1141 gaagccccac ctcccgggcc tggtagtcgg cccccgctcc caggccgtac gacgtggtcc
     1201 ggcaccgagc gtcagtcgcc agtttcgcga gcttcgcatc gtcggcccaa tagaccatgc
     1261 gaccatgctc gggcaactgc gcgatgaacc tgcggaagac atcggctacg tcgtcgacgt
     1321 ggcggtagtg actgtggtga tcgagctcca ggttcgtgaa caccgctact tcgggacgca
     1381 ggtagaggag ggatccatcg gactcgtcgg cctcgcacac catgatctcg cccgcgccgt
     1441 gccgtgcgtt cgaaccgagg tcgttgagtt cgccccccac caagaaggaa gggtcgaggc
     1501 caacatccat gagggtgcgg ctgatcatcg aggtggtcgt ggtcttcccg tgcgtgccac
     1561 agacggcgac gcctcggcgg ccctccatga cccaggccag cgcctgagcc cttctcacca
     1621 cggggaaccc acggcgacgg gcagcgctca actcacggtt gtgttcgggt atcgccgagg
     1681 agatgaccac gacatcaggg tggtcgaggt tgcgcggatc atgccccaag gtaacggtca
     1741 caccggcctc ctccaagagg ccggtgtagc gtgacgactt gaggtccgag ccggtcacct
     1801 cgtacccgcg ccggtgaagc acgagtgcga tgccgctcat ccccgcaccg ccgataccga
     1861 tgaagtggag ccgacgcagg cccgagagcc gcagatctgg ctggaagagt gtgctgttcg
     1921 tcatagtgtg ggtgcccgcg gagggcctgc caattgtact atctcgtcgg cgatgcgggt
     1981 agcggcgtcg ggacgcgcga gggcggccgc tcgttcgcgc atgcccgcgt gcctcctcgg
     2041 ttccagcacc cgcgccacct cggccgccaa cctctcgcca gagagttccg cgtcgggcag
     2101 cagcagggcc gccccctcgg cctgcagccc gagcgcattc ttggtctggt gatcggcggt
     2161 ggcggccggg aagggcacga gcaccgacgg tatcccgcgc gccagcagct cggcgacgga
     2221 gcctcccgcg cgggccaccg ccacgtccgc ggccgcaaga gccaactcga agtcgtccag
     2281 ataggcgtgc gtctggtacc gggggttcgc gcactcgacc gcgcgcctgc cccggagagc
     2341 ttcggcgacc cgctcccgat cggccgtgcc cgttacatgg atgaccgaga acggtgtctc
     2401 gccccgggcg aacgcctcga tcgcagccgt attcatgctc ctggcaccca gactcccgcc
     2461 gaacaccagg accacaggaa gtgccgggtc cagggagaag cgctccagac cctgctcgcg
     2521 cgtcgccgtc agcagcgacg gccgtacggg tcgcccggtg tagacgaact tccctcccac
     2581 gcgacccggg atggggaacg agagacagac gcggtcgacg agcatgctca agatcctgtt
     2641 ggtccaccct agatgggcgt ccatctcgag agcgacgctg gggatgcggg cgacggccgc
     2701 ctcggccacc accgggccgg aagcgtaggc acccaccccc accacgcacg cgggacgcag
     2761 tcggcgcagg gtggcccacg cgtcaacggc cgcgaccggg agcgacgcca acgtgcgcag
     2821 ggtggtcaac ccgagccgac gctcgaaccc gcggatgcgt acgctggaga acggataccc
     2881 cgcccgggtg acaagtccgc tctccatgcc gtgcggaccg ccgcagaagt ggactgcgta
     2941 tccgcgttcg gtgagaacgt cagcgatggc cagggcgggc accacgtggc ccgccgtacc
     3001 tccgccggct atcaatatgc gtgacattgg gtggctcccg acgctcgatg tgcgccgcga
     3061 tgggggtcgc cacgggcccg aaacgagcca cggagagcat gatccccacc gcgatcaaca
     3121 caatcagaag attggtccta ccgtaactga tgaagggtag gggcacccct gtgagcggca
     3181 gcgcgcccat gacccctccc atgttgatcc cggcctgccc cacgatgaga aacgtgcagc
     3241 cggccacgag atatctgccg aacggctcgg cgcagcgacg ggcaagcgcc agtccactcg
     3301 cggcgaacac ggcgaacaat gctatgacca ggcccacacc caccaggccc aactcctcgc
     3361 ccaacacggc caggatcatg tcggtgtggg cctcggggag atagctgaac ttctgcacgc
     3421 tcaatccggg gccgacgccg aaccacccgc cgctccccat ggcgaggaag gactgcacta
     3481 tctggaaccc tgagtcctgc gcctgagcga acgggtcgag gaaggcaaga aagcgctccc
     3541 gcctgtagtc ctcggtgaag atggccagca acacgagacc gaccccgccg cccgccagcc
     3601 ctatccaatc gcgcacccgc atcccggcta cccacagcag acccatggtc gctaccgcca
     3661 cgaccacgat ggtgcccagg tcgcgttgcc agaggatgag aacacagatc accgccacaa
     3721 tagctccgaa gggcagcacg aggtctttgg acgaccccga ctgcgcccgc ttggaggcga
     3781 gaaggtgcga gccgaccagt atcaccgcca acttcgcaac ttccgacggc tgaagcggca
     3841 aagggcccag gtcgagccac cgggacgcgc cgccggcgga ccgaccgacg ccgggcaggt
     3901 gcacggcgag caagagtacg accgttacgg ccacaccgaa cagcgcgagt cgccgccaat
     3961 ggcggtagtc cacccgtgag acgaccacca gacacactac gccggcaatg gcgacgagcg
     4021 actgctggcg caggtagaag ttcaagtctc cgtccgtgcg gaagaatccc accgcggacg
     4081 agaccgagta catcatcacc aatccgatgg cgagcaggcc gagcgctgag gcgatcagta
     4141 cttggtcggc gcgcagccgg aaggcggagc gcccgagagc gtcagcatcc tcggcgacgt
     4201 cgcgctcagc ggcggcctcg cggatcgagg cctgccgccg ggtctccgcc cggcggagaa
     4261 cggaacggcc gtcgccgccc tgctgggcgg cgacgtgccg attctgattc cgcgagttca
     4321 ctcccggtcc gccctcagtc tctggacgat ctgcatgaaa tgcgcgcctc tctcttcgaa
     4381 atcggagtac tggtcgaagc tggcgcacgc cggcgccagc agaaccgtat ccccaggagt
     4441 ggcggatgcc acggcgtccg ccaccgccct ttccaggtct ccctcatccg aaggcaccgg
     4501 aacggtctcc atgccctcct ccaccctcac ccgctcgaac gccgcccgga tgagaggcgc
     4561 ggcctctccg atcaagagca cgctcctgca acccaccgcg cacgcgcgtg ccacgggacg
     4621 gtagtccgat gctttgtccc tgcccccgag gatcaggtgt gtcccggtcg gataggcgtc
     4681 gagggccttc agagtggcct ccacgttcgt agccttgctg tcgttcacat acgccacgcc
     4741 gtccactact cctgccggct gtagtctgtg ggggaccccg ggaaaggtgc ggagcgcctc
     4801 ctcgacggcc tggctcggca gccctcgggc cagcgccacc gtcgaggctc ccagacagtt
     4861 ctccatgttg tgccggccgc gcaaggggat ggagtccgcc gggatgccgc cgccgagaag
     4921 gtgcagcgtg acgccatcga accaggcggc ggctcctggg gaccccagag tcgagaagaa
     4981 gaggaccgcg ggcccccctt cccgagccgc gacttcccgc ccaagcccgg ccaccgcagg
     5041 gtcgtcgagg ttgaacaccg cccagtcgtc cgaccgctgc cgagcgaaca tgttggcctt
     5101 gcaggccagg tagcgttcca tcgtgccgtg gcggtcgaga tggtcggggg tgaggttcag
     5161 cagcactccg gccgccggtc taaactcgtg tacgtcctct aactggaagc ttgagagctc
     5221 caccaccagc tcgcactccg ggccgaggcc acgcgccgcg gaagtgatgg ccgtcccgat
     5281 gttcccaagg acgagggccg gatgccccgc cgtcttgaac atgtggccga ggagagcggt
     5341 ggtggtggtc ttcccgttgg tgccggtgac tgcgctgaag ggggccggaa gaagggcgta
     5401 ggcgagttcc gcctcactcc acacggggat gccgacgtga tgggcggctg tgagcagcgg
     5461 cacctcggcc gggacgccag ggctcttcac cagcagatct acgccctcaa gctgatcagg
     5521 tgactgcggg cccggcagca gccgcgcgcc ggcgagcagt gccggctcga tcccggggag
     5581 ttcggcgagg cccctgcggt ccgaaaggac gacttcgcgc cccaagcccg ccagcagggt
     5641 cgcggccgcg acccctgaac gacccgcgcc cacgaccaac accctgcgca cgtcggcgcg
     5701 cagccccaca gggtggtcac ctgcacaatc ccggtgcatc agagggtgtc gacgtagaag
     5761 atggtgaatc cggccgcggc gaagatggcg gtgacgatcc agaagcggac gatgatcttg
     5821 gtctccgacc agtctttgag ttcgaaatga tgatggatag gagccatcag gaatacccgc
     5881 ctcttgaaga cgcggaagct gatcacctgg atgatgacgc tgagcgcctc gaccacgaag
     5941 atcccgccga tgaccacgag cagcaactcg gtgccggtga ccaccgccag gccggccaag
     6001 gcagccccca gcgccaggga gccggtgtcg cccatgaaca cctcggccgg tacggagttg
     6061 taccagagga atccgacgca cgcgcccatc aggctggccg agaggatcgc gagatccggc
     6121 tcgtggagca tgaaggtgat ccccgtgtag gccaggagac acgtggccac ggtgcccgca
     6181 gccaggccgt cgagcccatc ggtgatgttc accgagttgg cgaatcccga gatgacgaag
     6241 tacacgacca ggaagtacac acccccgaag gcgagttcct tgttcagaag cggcacgtgc
     6301 acattgctgt ctacgccggc gaacctgatg gccacccagc ccacgacgag caccagcagc
     6361 agctggagac tcatcttggt cttgccgctg atcccaagag agcgcttgcg tctgaccttc
     6421 gccaggtcgt ccaggagacc gatggcgccc gagccgaacg tcaacacgaa gaccaccaag
     6481 ctggggacgg tcttgcgggc cagtatcatg taggggattg ccgcggcggc caggatcaac
     6541 agacctccca tcgtgggggt gccctgcttc acctggtgac gctccgggcc gacgtcgcgg
     6601 atgttctggc cgatgcccct atcgcgcagc caccgaatga acctgggccc ggcgaccacg
     6661 gccaagacca tcgcaagcag cccggcggcc aggacgcgga tcatcgtgcg ccgcccactt
     6721 cgtccgagca ccgctcgtgc tctaggcggg ccaccagtcg ctcgactagg cggtccagct
     6781 tcattccccg ggagcccttc accaggacca cgtcgccggg ccgcaggtag gcgacgatgc
     6841 cgtcgacagc cgcatccgcc gtctcgctcc acacgcccgg ctgcatcccc gccaggggtt
     6901 cgaggcggtc tcgcagtgac tcccgaaacc cttggatggt gtggcgactc cggggtccga
     6961 caccgaagag ggccgccaca cccgtgcgcg ccgcgtgtct gcccacctca cggtggtact
     7021 catcggcatg ggggcccaac tcgagcatgt cgccgagcac tgcgatcggc ctgcggccgc
     7081 cggactccgc tgagatcgcg agatcgtcaa gagccagccg gacggcgacc gggttcgcgt
     7141 tgtacgtgtc gtcgacgatg agcacgccgc cggccaagac ctcgtcgccg cgcagggggg
     7201 tgaaatccac gctggagagc ccgctcacac actcggccac gggaaggcct gcggcgtagc
     7261 aggccgcggt cgcggccact gcgttccgga gtcgatgggc ggcgcggaag gcggtgccga
     7321 cctcggcctc cccccccggc caggtgaggc gcaataaagc gcccccgttg ccgtctctgc
     7381 gggctactcc gaccacgtcg gcggcgagag accgcgcctc cgagaggtca ggcccgatct
     7441 ccggtgaagc cgcccgcgcg tcggtgaagt cgaacgtgac cacgcggacc cccgacagcc
     7501 gagcggctgc ggcaagctcg aacgcatcca ccggcacaac tgcagcgccg ccggcacgca
     7561 ctccctcgaa gatctccgcc ttcgccgcga gcacgtcttc catgcggccg atcaactcca
     7621 gatgcacggg cgcgatggcg gtgatcacgg cgacatccgg ctcggccgtc aacgtgagcg
     7681 acctgatctg cccctttccg cgcatgccca tctcgaccac gaccacctcg gtctcgggcg
     7741 tgatctcgag aagggtgagg gacactccga tctcgttgtt ctcgttagcg gaggtagcca
     7801 ccactctacg gacgaccgct gccatgccgc tgacaagatc cttcgtgctg gtcttgccga
     7861 cgctccccgt gatcgccacg actgtggccg tggacgcgcg cctcacctcg cgcgccagcg
     7921 ccgcgagggc ggcaagcgtg tcgggtactc tgtgcagcgt ctggccgccg ggaggcaggt
     7981 agtcgacttc gcccgcgatg gctgcgccga gttcgaccac ggccgcgccg gcaccgccgg
     8041 ccagcgcggc cggcacgaac tggttgccgt cgtagttgtc tccccgcagt gcgatgaaga
     8101 ggtcgccctt gaccagtgtg cgcgtgtcgg tggagatgcc cgtcactgga gcgctcacgg
     8161 tatcttctcc gagagcgcgc gccgcgatct cggccgagag ctcgatcatg aggactccgc
     8221 catgatcacg cgcacgaggg cgcgcaccac ctcgcggtcg tcgaagggga tcaccctgtc
     8281 ggcgaactcc tgcccgctct cgtgcccctt gcccaggatc agcacgagat caccggcacc
     8341 cgcccggcgc agcgccgcct cgatggccac gcgccgatcg acctcgacca ccgcttcgcc
     8401 ggggcattcg agacccgcga ggatgtcgtc caagatcgcg gccggatcct cgctccgggg
     8461 attgtccgag gtgagcactg ccacctcgca ccgggattcg agcgcgcgcc ccatgagcgg
     8521 ccgcttcgct ctgtcccgat cacccccgca gccgagaacg ccgatcaccc gacctcgggc
     8581 aatggagcgc gccatgtcaa gcaccctctc cacggagtcg ggcgtgtgcg cgtaatccac
     8641 cgccaccgtg aaggcctggc cctcgtcgat gcgctcaagg cgacccggca cgcccggaaa
     8701 gaacgggagt gcgtcgagca tggcctgagg gtccgcccca agtgagaggc agatgcccag
     8761 gcccacggct atgttctcca ggttgtaccg ccccagaagt ctcgtgctca actcgaggct
     8821 cagctcctca cccttcacga caggctcacc tctaccccgc cgcaccgcct ccagggcacg
     8881 atgcttcaga accaggctcc ccgttgcgcc cctttcgtcc agccgcgcat tgcgaatctc
     8941 gaggtccggg gtacgacgtc ccgggtcggt gagaccatcg gacgcgacag ccacggtgag
     9001 gacgcgatcg gggaccagtc gctccaccag cagtcggcca aaaggatcac ccacgttgat
     9061 gacagcgggc gggcggttgc gcgcgtagtc gctgtcgagg aagaggcgac tcttcgaagc
     9121 gaagtacgcc tccaaagtgc cgtgatagtc gaggtggtcc tgagtgaggt tggtgaaggc
     9181 gacggcggag aagtggatcc ccagcgtccg ctcgagatca agagcgtgcg atgagacttc
     9241 catcacagcc gcctgatccc ccgcgtcgac catgagggca aggtcctgct gcacatccac
     9301 cgcctcgggg gtcgtacgtt cggccggaaa gacctctccc ccgatcctcc gttccaccgt
     9361 tccgagaagg ccggcgcgcc gaccggtctc gcccaagaag aaggcacaca ggaagccggt
     9421 ggtggtcttg ccgttggttc cggttattcc gaccgtcgcc aggcgtgcac tcgggtgttc
     9481 gaagaggctg gaggcgacca ggcccatagc ccgacgcacg gagggtacca ccacctgggt
     9541 gacgggcagg tccagaggcc gttcgacgca cagggccacg gcgccccgcg aaaccgcatc
     9601 gggagcgaaa tcatgcccat cacgcacgaa cccgggaacg cagaagaaca gatggcccgg
     9661 accggcgaga gagctgcggt aggagagatg cgagacgtcg gtggatgggt ctccctcgac
     9721 ctcgaaaccc cctacctcac ggaaaaggtc tgcgagtctc acggctttcg attctaccac
     9781 agggctctgg gccgacgatc gcggcgcggg ctcactgctg tccggtcgga gggatctcca
     9841 gacgcctcag agcgaagtcc gcgatctcgg caaatgctgg tgcggctacg acggacccgt
     9901 agtactggag cgtgggttcg tctatgacga ccaatatgac cagggcgggg tcgtccgcgg
     9961 ggatcatgcc tatgaaggag gcgacgaagg tgttattgga atatccccga ccattcggca
    10021 gcaccttctg cgccgtaccc gtcttcccag ccaccacgta cccgttcacc tgggcccttt
    10081 ggccggtgcc gtcggtcacg gtcacacgca acatgtctct cagctgccgg gccactcctg
    10141 cgctcaccac gcggtgcgac ggcgtggtca ccgccccttt ggtgagatgc ggctgcacgt
    10201 acacgccttc gttggcgatc gctccgtagg caacggccaa ctggagagga gtgaccgaga
    10261 taccttgtcc catgggaacg ttcgcaatgg tcgaccccga ccactggtcg ggcgtcagca
    10321 tcgacccgga cgcctcgccg gggaagtcga tgccggtgcg agtggtgaac ccgaaaccat
    10381 agatcatgtc ggcgagcctc tccttgccca cctccagtcc gagagtgacc gcccccacgt
    10441 tcgaggactg cgccaggatc tcggtgaccg tcatcgaacg tatcgccggg atatcctcgt
    10501 gggcctcgtg caccgtccga tcgtgcactt tgatactcgg cgcaagatcg aacgaagtgt
    10561 cgggctgcac gagcccggcc tccagcgccg ccgccaccgc cacgaccttg aacgtggagc
    10621 cgggttcgta ctggtcggtg accgccgcgt tcctctggtc ggcctccttc tgcaccccga
    10681 actcgttggc gtcgaagtag gggacgttcg ccatcgccag gatctccccc gtcttcgggt
    10741 cgagcacgat cgcggtcccc ttgagggctc cgaacgtctc taccgcgtcg gcgacggcct
    10801 gctcggccgc gaactggatg tcctcgtcga tggtcaacac gaccgaacga ccgtcccgcc
    10861 ccgcctcgtc gaagagcaca cccagagtgc ggccgaaggg atcgcccacg atgctccgct
    10921 tgccgctgac gcctgtcagc tgttcatcgt attgcttctc caccccggcc agtccgagat
    10981 tgtcggttcc gacaaagccg agaagctgag gggcgagcgc ccccttcggg t
//
LOCUS       DASRMJ010000082         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_150445, whole genome shotgun sequence.
ACCESSION   DASRMJ010000082 DASRMJ010000000
VERSION     DASRMJ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150445"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFD74_02405"
     CDS             complement(<1..333)
                     /locus_tag="VFD74_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48439.1"
                     /translation="MGDRAGAAASHGAGRDAGLKTGARQMLLLAGLTWREGIRRRMIL
                     VGFVLTVVFVALYGTGIYFAFRDFDVMSGGTAGGAGAIAAALDSNFFRDLAAYQMLSF
                     GLFISTFLN"
     gene            complement(442..1464)
                     /locus_tag="VFD74_02410"
     CDS             complement(442..1464)
                     /locus_tag="VFD74_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48440.1"
                     /translation="MSVGAPHAPATRAVATGAAVIECQGIRKRYGSTAALAGVDLRVH
                     AGSVFGFLGPNGAGKSTLVKILTGLVRPTAGSVRVVGGRPGTKAVQRRLGYLPEHFRF
                     PYWLTGRELLGVHGGLADVEPNDSYLLELVGLADAADRRIGEYSKGMQQRLGLAQALI
                     GDPEVVFLDEPTSALDPLGRLDVRDILLHLRDRGMTVFLNSHLLTEVERVCDRLAVID
                     HGEIIAQGTTDELLDRPGLRVRATGVTIDTLALLAARTPGSGAAAPAISYEGGWLEIP
                     DVGVEDIPGVVQVLVESGARVYEVGVARRSLEDAFVRLVRGHQQATRQDAPRAAASHP
                     PEDGDS"
     gene            complement(1461..2489)
                     /locus_tag="VFD74_02415"
     CDS             complement(1461..2489)
                     /locus_tag="VFD74_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK48441.1"
                     /translation="MTAANAPGERHGSAGRDGAGARDQAAGRDPGASRRGALDEAPGR
                     DETPGDPRGLTGAHVAGTETGAVDPATAACKRGRAEGGARGPRGLAAAGGRFRHVWFR
                     DAYVFVKLWKTNLFPPLIEPYLYLLALGLGVGFYVNQMDGVDYLTFVAPGIIVTTSIM
                     QATFECTYGSYFRMAFQSTFDAIISTPVNPEEVSLGEICWGATRSVINAAALMVSLAI
                     FGRLDLIWAPAILALQFLIGMQFGAISLLITSKVRQTEYFNFFLSGVIFPAQMLTGAF
                     FPLSRIPEVLQPVAWAVPLTSAIDLTRGMMLDRLGMHSLLELAYVAVTTLVFVELALR
                     SMRRRLIG"
     gene            complement(2486..>3473)
                     /locus_tag="VFD74_02420"
     CDS             complement(2486..>3473)
                     /locus_tag="VFD74_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48442.1"
                     /translation="RGPRRQGGCFHGRTGTMVIMPTPTRTHALIEARGLFKCYDDLVA
                     VNDVDFHVLPRDFLGFLGPNGAGKTTLMKMIYCLAEPTAGNLSVFDTDVLADPRSIKS
                     RLGVVPQENNLDPDLSVWDNLMIYARYFGLRGPEVKARAKESLEFMELWDRRDTVIMH
                     LSGGMKRRLVIARALLNDPDIVILDEPTTGLDPQVRHLIWDRLLELRDQGLTFLLTTH
                     YMEEAEKLCDRLYIMDRGRIVTSGKPTELVRRHVGEFVLEIVADQETERQILDERPDM
                     ACQRSGQRLYLYGASSEELAPFVARYPERETRLRPASVEDVFLKLTGREIRE"
BASE COUNT          565 a         1175 c         1188 g          545 t
ORIGIN      
        1 gttgaggaag gtgctgataa agaggccgaa gctgagcatc tggtaggcgg cgaggtcgcg
       61 gaagaagttc gaatccaggg cggccgcgat cgcccccgcg ccaccggccg tgccgccgct
      121 catgacgtcg aagtcgcgga aggcaaagta gatccccgtg ccatacagcg cgacgaagac
      181 cacggtcagc acgaacccca ccaggatcat gcggcgcctg atgccctcac gccaggtcag
      241 ccccgccaag aggagcatct ggcgcgctcc ggtcttgagt cctgcgtcgc ggcccgctcc
      301 gtggctcgcc gccgcccctg cgcggtctcc cactctgcgg ctcgccgccc gcccctgcgc
      361 ggtctcccgc gccgcgtcct gcgccgcccc cggcgccgac atgcgtcggc ggctgcggtg
      421 cctgactcgg tctccagcgc gtcacgagtc gccgtcctcg ggtgggtggc ttgcggccgc
      481 gcgcggcgcg tcctgccgag tggcctgctg gtggccgcgc accagccgga cgaaggcgtc
      541 ctccagcgac cggcgcgcca cccctacttc gtacacccgg gcgcctgact ccactaggac
      601 ctgcacgact ccggggatgt cttcgacgcc gacatccggt atctccagcc accctccctc
      661 ataggagatc gccggtgccg ccgccccaga ccccggcgtg cgggccgcca gcagagccag
      721 cgtgtcgatg gtgaccccgg tggctcggac gcgcaacccg ggacggtcca ggagttcgtc
      781 ggttgtgccc tgggcgatga tctccccgtg gtctatcacg gccaggcggt cgcacacgcg
      841 ctccacctcg gtgagcaagt ggctgttcaa gaacacggtc atgcctcggt cgcgcaggtg
      901 caggaggatg tcgcgcacgt ccaagcggcc gagggggtcg agcgcactgg tgggttcgtc
      961 gaggaacacc acctccgggt caccgatcag ggcctgggcc aagcccagcc gctgctgcat
     1021 ccccttggag tactcgccta tcctgcggtc ggctgcatcc gccaggccca ccaactcgag
     1081 caggtaggag tcgttgggct ccacgtccgc aaggcccccg tggacgccta gtagctcgcg
     1141 gccggtgagc caatagggga agcggaagtg ctcgggtagg tagcctagcc gacgttgcac
     1201 ggctttcgtg cccgggcggc cgcccaccac ccggacgctc cccgccgtgg gtcgaaccag
     1261 tccggtgagg atcttgacga gcgtcgactt gcccgcgccg ttcggcccca agaagccgaa
     1321 gacgctcccg gcgtggacgc gtagatccac cccggccagg gccgccgtgg agccatagcg
     1381 cttcctgatt ccttggcact cgatcacggc agcgcctgtc gcgaccgcgc gcgtcgcggg
     1441 agcgtgcggg gctccaacgc tcatccgatc agcctccggc gcatgcttcg caacgccaac
     1501 tccacgaaca ccaacgtggt gaccgcgaca taggccaact cgagcagaga gtgcatgccc
     1561 aagcggtcga gcatcatgcc ccgggtcagg tcgatggcgg aggtgagcgg caccgcccaa
     1621 gccaccggtt gtagcacctc gggaatgcgc gagagtggga agaaggcgcc cgtgagcatc
     1681 tgagcgggga agatgacacc gcttaggaag aagttgaagt actcggtctg acgcaccttg
     1741 ctggtgatga gcagagagat ggctccgaac tgcatgccga tcaagaactg cagggccagt
     1801 atggccggag cccagatcaa atcgagcctt ccgaagatgg ccaggctgac catcagcgcc
     1861 gcggcgttga tcaccgagcg tgtcgcgccc cagcagatct ctcccaggct cacctcctcg
     1921 gggttcaccg gcgtggagat gatggcgtcg aaggtgctct ggaaggccat gcggaagtac
     1981 gagccgtagg tgcactcgaa ggtcgcctgc atgatggagg tggtaactat gatgccggga
     2041 gccacgaagg tgaggtagtc gacgccgtcc atctggttca cgtagaagcc cacgcccagt
     2101 cccagagcga gcaggtagag gtagggctcg atgagcggcg ggaagaggtt ggtcttccac
     2161 aacttgacga acacgtaggc gtcacggaac cacacgtggc ggaagcggcc gccggccgcc
     2221 gctaagccgc gcgggccgcg ggcgccgcct tcagcgcggc cgcgtttgca ggccgcggtg
     2281 gcggggtcca cggcgccggt ctcggtgccg gcgacgtgcg ctccggtcag tccgcggggg
     2341 tcgcccggcg tttcgtcgcg gccgggcgcc tcgtcgagcg ccccgcggcg acttgcgccc
     2401 gggtcgcgcc cggccgcctg gtcgcgcgcg cccgcgccgt cgcggccggc cgacccgtgg
     2461 cgttcaccgg gtgcgttggc ggccgtcatt cgcggatctc ccggccggtg agcttcaaga
     2521 agacgtcttc caccgaggcc gggcgcaggc gggtctcgcg ctccggatag cgggcgacga
     2581 acggtgcgag ctcctcgctc gaagcaccgt agaggtagag gcgctgaccg cttctctggc
     2641 aggccatatc gggacgctcg tcgaggatct gccgctcggt ctcttggtcg gcgacgatct
     2701 cgagaacgaa ctcacccacg tgacgccgca ccaactccgt cggcttcccg ctggtgacga
     2761 tgcgtccgcg gtccatgatg tacaggcgat cgcagagctt ctcggcctcc tccatgtagt
     2821 gagtggtgag gaggaaggtg aggccttggt cgcggagctc cagcagccgg tcccatatca
     2881 ggtggcgcac ctgcgggtcg agcccggtgg tcggctcgtc gaggatcacg atatcggggt
     2941 cgttcagcag cgcgcgggcg atcaccagac ggcgcttcat gccgcccgag aggtgcatga
     3001 tcaccgtgtc ccgccggtcc cagagttcca tgaactccag cgactccttg gcccgagcct
     3061 tgacctccgg gccgcgcagc ccgaagtagc gcgcgtagat catgaggttg tcccacaccg
     3121 agaggtccgg gtcgaggttg ttctcctggg gcacgactcc caatctcgac ttgatgctgc
     3181 gggggtccgc caagacgtcg gtgtcgaaga ccgacaggtt gccggcggtc ggttcggcca
     3241 gacagtagat catcttcatg agtgtggtct tgcccgcgcc gttcggcccc aggaatccca
     3301 ggaagtcgcg cggcagaaca tggaagtcca cgtcgttgac cgccacgagg tcgtcatagc
     3361 acttgaacag accccgagcc tcgatcagag cgtgcgtgcg ggtgggagtc ggcataatca
     3421 ccatggtacc agtgcggccg tggaagcacc cgccttgacg tcggggcccg cgg
//
LOCUS       DASRMJ010000083         7023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_511990, whole genome shotgun sequence.
ACCESSION   DASRMJ010000083 DASRMJ010000000
VERSION     DASRMJ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511990"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            958..2082
                     /locus_tag="VFD74_02425"
     CDS             958..2082
                     /locus_tag="VFD74_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZK48443.1"
                     /translation="MSRSKTVLAVGFILLGLFVVLVGCGGGEAATTTSQPASGSETTS
                     STSGETGTTQAAIEAELPGVLRLGTHPMGSTYNALGAGLATVLSQNTSIEVKAIATSG
                     PSEWEPMFASGDMDMGVANSFDSGMGYLGQSDYADISNGEGFPIRLITVGSLNRVGVV
                     VPRDSDIKTAADLKGKRFVGEITGSAGITAMATAYLANQGLSPSDVKMVAVPGIADGV
                     RAVIEGRADATTVAYGTPMVQELDASKGARFIGIDPSPEAMARLQAIFPAKAVEVKPG
                     ANAVGIAEPIFLMGYDNYLVARADLSDAAAYAVTKALWEHQAELKPIHAAFTSWTTDR
                     YVSDAATVPYHPGAIQFYKEVGVWTSAMDELQERLLSAKK"
     gene            2234..4114
                     /locus_tag="VFD74_02430"
     CDS             2234..4114
                     /locus_tag="VFD74_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HZK48444.1"
                     /translation="MGIAVPAAGMFFILDVPMYLTGKSFFTQQYLGLFLGLVLALAFL
                     LLPMSKSAPRDRVPWYDLVFAVASLAVGAYVLFLYPQLIRTMGIVSPDKIVLGLLAIF
                     LVLEATRRVTGWPLTIIVLVFIAYGRYGYLLPDVVAGSDASWGRLINQLFLGSGSLYG
                     IALRVAALIVFAFVFLAHGLFGYGGGDFLFGVAQTSMGRYRGGPAKMAVLASAFFGML
                     SGSAVANVAGTGTITIPLMKKNGYSPEFASAVEAVASTGGTLTPPVMGAAAFIIAEFL
                     GVGYSEVVIIAIVPAALYYLGLFIQIDLRAVKKGLKGLPNVTRLPVGQLFTQGWLYLA
                     ALGILMYGLFFLYLRPEVAALYALGTLIVLGSVSKRTRQGLTGLLTILRTTGRGMLEI
                     TVIGAAAGIVIGVVNYTGLGLSLSRLLTDLSGGSLLLLALMTAVASIILGMGIPVTAS
                     YLFLAVLAAPALVQRGVPPLVAHMFIFYFGAFSFITPPICLAVFTAASIGGANQMKTA
                     LQAMRLAVAGYLVPFIFLFDRGVVLQGTPSQIAVGVLSAIAAIIAFCFALEGYFLRRL
                     GWRKRVLYVAAGTALLIPGHWSLNVVGGGAMAGLMLLDMVWVRKHAKDSRVKAMVGSE
                     SA"
     gene            4166..5575
                     /locus_tag="VFD74_02435"
     CDS             4166..5575
                     /locus_tag="VFD74_02435"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HZK48445.1"
                     /translation="MARDLREFVRQVERELPSEYVSVSRKVRPTKFEVTALLETLTEA
                     GHFPMVEFKSTEDAHGNDVDIPIVSNVFASRKRCALALGHDPDKHKTELSLEYARLAG
                     RTIAPETIDSSEAPVKQVVLRGEQVDMGGLPVVKHYEMDLSPVYTMALAMKDPDTGAY
                     NVSFIKGFYKNDPRKLGISIHSPHLERVLRKYEERGEAAPAAFILGHHPAFYLGALAL
                     SPYDSDDYATIGSFMREPLRLTPSTTWGEDFMVPADAEIVVEGEIPPGAREVVDPFGE
                     VTRHYQAQCLRQSMQVKALTRRTDAYLQDIFSGHEGHWNLGGIPKEGSVFNAVNARVG
                     NVTAVHMPHSGVSRLACYVAINKQKEGDGKVAGLAALLESWTFQAIVVVDADIDVFNE
                     KEVVWALLTMVDPQRDVTYVRNMSTTFTTAMGHNKVVIDATKPLDRAFPTRFRVPPAA
                     MDGVRLEDWIDDWTGRSVD"
     gene            5576..5722
                     /locus_tag="VFD74_02440"
     CDS             5576..5722
                     /locus_tag="VFD74_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48446.1"
                     /translation="MDKVFWVTCPDCAGRFFCDMELRRSGHKLICPFCQNEFLDEESP
                     EIEE"
     gene            complement(5825..6763)
                     /locus_tag="VFD74_02445"
                     /pseudo
     CDS             complement(5825..6763)
                     /locus_tag="VFD74_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
     gene            complement(6848..6979)
                     /locus_tag="VFD74_02450"
     CDS             complement(6848..6979)
                     /locus_tag="VFD74_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48447.1"
                     /translation="MNDARSLLDEMRVSHAEHLPLVAAFPERMVRISAELTTGIGRS"
BASE COUNT         1201 a         2171 c         2282 g         1369 t
ORIGIN      
        1 tcccgaagga agtccatctc ttctccggcc agaagcttgt ccagaagggc gcctggtgtc
       61 aggatggaat cggccatcgt cgtgtctcct ctcacggtgc ggttggtagc cacacctaca
      121 ggaacacacg atggcctttc tctgcaagga ggtcgtcacc cggctcttgc tccctccgtt
      181 gttacaccac gtccggggac actatccatg cgctggcggg gagtcggtcc cgatcacggg
      241 tatcccgacg ccgccgcccg agcagcacac ggtccctttc gcgtactcgc cgcctcaccc
      301 ggacattggc ccggtatctg aggtggccgt gaacccgcgg ttcactcttc ggtctgataa
      361 tccacacaga cgagaatgtg cttcttgagt tccgggcggc gggccttctc ggaggcaagg
      421 ggaggggcgg tctctcctgg tcatcaattg cgagcaagtg ggggccgtcc ttgctttctg
      481 acggccgtgg gacgagggcc tgtcgaccac aaacgcgagg cggatccccc cgtctgtggc
      541 gtatcaaata ctgattcctg cttcccctac gcttccccga agtgcaagat ccatagttct
      601 ccctgctcac gcgggattca catctgattt ctactcagca ggttgggggt tcgattccct
      661 tcgccggctt cgcgcttgtt tggcggctac ttctctatat agccattgaa gttcttgacc
      721 gaccggggga ccacctgcta gcatgaggtc aatgacgtgg gggttcttgg gggctaccct
      781 gggggggacg ttgctcgact gtgcgtggtc gaatccgggc cgcggcgccg tcgactttga
      841 tcgacccctc acaataccct gagcctgggg gcgaccatgg attgaccctg ccagcacctc
      901 acggagagag agttccaccg aaggttgcag tgacatggat gtaggggggt acgaatcgtg
      961 agtagaagca agactgttct cgccgtcgga tttatcctgc tcgggctgtt cgtggtgctc
     1021 gtagggtgcg gagggggcga ggcagcgacg acgaccagcc agccagcctc gggctccgag
     1081 accacgagtt ccaccagcgg ggagacgggc actacccaag ctgcgatcga agcagagcta
     1141 ccgggcgtgc tgagactcgg aacgcaccca atggggtcca cctacaacgc tctcggcgcc
     1201 ggactggcta ccgtcttgag ccagaatacg tccattgaag tcaaggccat tgccacctcg
     1261 ggcccttccg aatgggagcc catgtttgcg agcggcgaca tggacatggg agtggcgaac
     1321 agtttcgata gcgggatggg atatctgggt cagagcgact acgccgacat ctccaacggc
     1381 gaagggtttc cgatccgact tatcacggtg ggttctttga accgggtggg agtggtcgtc
     1441 cccagggact ccgacatcaa gacggcagcc gatctcaagg gcaagaggtt cgtgggcgag
     1501 atcacgggaa gcgctggtat caccgccatg gcgacggcct acctggcgaa ccagggactg
     1561 tccccgagcg acgtcaagat ggtggctgtt cccgggatcg ctgacggagt gagggcggtc
     1621 atcgaaggcc gagcagacgc tacgactgtc gcctacggga ccccgatggt ccaagaactc
     1681 gacgcctcga agggcgcacg cttcataggc atcgatcctt ccccggaagc gatggctcga
     1741 ctccaggcta tctttcccgc gaaggcggtg gaggtgaagc cgggggccaa tgctgtcggg
     1801 atagctgaac cgatcttcct catgggctac gacaactacc tggtcgcccg ggcggacctg
     1861 tcagatgccg cggcctacgc ggtcaccaag gcgctttggg agcaccaggc agagttgaag
     1921 ccgatccacg ccgccttcac cagctggacc acggaccgct acgtctcgga cgctgcgacc
     1981 gtgccctatc acccgggcgc catccagttc tacaaggagg taggcgtctg gacctcggcc
     2041 atggatgagc tccaagagag actcctgagc gccaagaagt agcaggactg ggagactcgt
     2101 cagagagagg ggacccgggg ctccgggtcc cctctgtcat ccatcagcgc aaagcagggg
     2161 gggcttcttg ccggcagagg agaagagccg tttccggact ctatcgggtc cgtggcacaa
     2221 ggcggagtgg gtcttgggaa tcgccgttcc ggcggcgggg atgttcttca tcctcgacgt
     2281 gcccatgtat ctcacgggga agtcgttctt cacccaacag taccttggcc tgttcctcgg
     2341 actggttctg gcgctcgcct ttctgctgct gccgatgtcg aaatccgctc ctcgggaccg
     2401 cgtcccctgg tacgatctgg tgttcgccgt agcctccttg gctgtcgggg catatgtgct
     2461 cttcttgtat cctcaactga tacggactat ggggatcgtc tcgcctgaca agatcgtcct
     2521 cggccttctg gcgatcttct tggtcttgga ggccaccaga cgcgtgacag ggtggcctct
     2581 cacgatcatc gtgctcgtct tcatcgcgta cggccggtat ggctacctgc tccccgacgt
     2641 cgtggccggt tccgatgcca gctgggggcg cctgatcaac cagctcttcc taggctccgg
     2701 ctcactctac ggcatcgcac tccgggtcgc agcgctcatc gtctttgcgt tcgtctttct
     2761 ggcgcatggc ctgttcggct acggcggcgg cgacttcttg ttcggggtgg ctcagacttc
     2821 catgggccgg tacaggggag gaccggccaa gatggccgtt cttgccagcg ccttcttcgg
     2881 gatgctctcg gggagcgccg tggcgaacgt ggctggtacg gggacgatca cgatcccgct
     2941 catgaagaag aacggctact ctcccgaatt cgccagtgcg gtggaggcgg tagcctccac
     3001 cggcgggacc ctcacgcctc cggtcatggg cgctgccgcc ttcatcatcg ccgagttcct
     3061 gggcgtcggc tactccgaag ttgtgataat cgccatcgtg cccgccgcac tgtactacct
     3121 gggtctgttc attcagatcg atctccgcgc agtcaagaaa ggcctgaaag ggttgcccaa
     3181 cgtcacgcgg ttaccggtgg ggcaattgtt cacgcagggt tggctgtacc tagcggcgct
     3241 cggaatcctg atgtacggct tgttcttcct gtacctgcgt cccgaggtcg cggcgctcta
     3301 tgcgctgggc acgctgatcg tgctcggcag cgtgagcaag aggacgcgcc agggattgac
     3361 ggggctgctc accattcttc ggaccaccgg ccggggcatg cttgagatca ccgtcatcgg
     3421 cgcggcggcc ggcatcgtca tcggggtggt gaactatacc ggcttgggtc tttcgctgtc
     3481 gcggctgctc accgatctgt ccggcggcag tcttctcctg ctggccctca tgaccgcagt
     3541 ggcgagcatc atactcggca tgggcatacc ggtcactgct tcctatctgt tcctggcggt
     3601 gctggccgcg ccggctctgg tccagcgggg cgtgccgcct ttggtggccc atatgttcat
     3661 cttctacttc ggcgcgtttt cgttcatcac cccaccgata tgtctggcgg tgttcaccgc
     3721 ggcctccatc ggaggggcca accagatgaa gacggccctg caggccatgc ggctcgccgt
     3781 ggcgggctat ctcgtgccgt tcatctttct gttcgatcga ggggtcgtcc tgcagggcac
     3841 cccgagtcag atcgcggtcg gcgtactctc ggcgatcgcg gcgatcatcg cattctgctt
     3901 cgcgctcgag ggctacttcc tgcgcaggct cgggtggcgg aaacgggtct tgtacgtggc
     3961 cgccgggact gcgctgctga taccgggtca ctggtcgctg aacgtggtcg ggggaggggc
     4021 gatggcaggt ttgatgctgc tggatatggt gtgggtccgc aagcacgcga aggattcacg
     4081 agtcaaggcc atggtgggga gcgagagcgc gtagaccgcg acggacgatc cgtgggggga
     4141 gtctgcgcag aaagaagagg gcggtatggc acgagatctt cgagagttcg ttaggcaggt
     4201 cgaacgggag ctgccttccg agtacgtcag tgtgagccgg aaggttcgtc ccacgaagtt
     4261 cgaagtgacc gccttgctcg agacgttgac cgaagcgggc cacttcccga tggtggagtt
     4321 caagagcacg gaagacgccc atgggaacga tgtagatatc ccgatcgtgt caaacgtatt
     4381 cgccagcagg aagcggtgcg cgctcgccct cggtcacgac cccgacaagc acaagaccga
     4441 actgagcctg gagtacgcac gtctggccgg gcggaccatc gcccccgaga cgatcgactc
     4501 ctcagaggct cccgtgaagc aggtggtgtt gcgcggcgag caggtggaca tgggcggcct
     4561 tccggtggtc aagcactacg agatggacct cagcccggtc tacacgatgg cgctcgccat
     4621 gaaggatccc gacaccggcg catacaacgt gtctttcatc aagggcttct acaagaacga
     4681 ccccaggaag ctcggcatct ccatccactc gccccacctg gagcgggtgc tgcggaaata
     4741 cgaggagcgc ggagaggccg ccccggcagc cttcatcctt ggacatcacc cggcgttcta
     4801 tctgggtgcg ctggcactct ccccctatga cagcgacgac tatgcaacca tcggctcatt
     4861 catgcgagaa ccactgcggc tcacaccgtc gaccacttgg ggcgaggatt tcatggtgcc
     4921 ggccgacgcg gagattgtcg tggagggtga gattccaccc ggggcccgtg aggtggtgga
     4981 ccccttcggt gaagtcacgc gtcactatca ggcccaatgc ctgcgtcaga gcatgcaggt
     5041 gaaggccttg accagacgca cggatgccta tcttcaagac atcttctccg gtcacgaggg
     5101 tcactggaac ctcggcggta ttcccaaaga gggcagcgtc ttcaatgcgg tgaacgccag
     5161 ggtgggcaac gtgaccgccg ttcacatgcc ccactcgggc gtatcccgcc tggcctgcta
     5221 cgttgccatc aacaagcaga aggaaggcga cggcaaagtc gccggtctgg ccgcgttgct
     5281 cgagtcgtgg acgttccagg cgatcgtggt cgtggacgcc gatatcgacg tcttcaacga
     5341 gaaggaggtg gtctgggcgc tgctgactat ggtcgatccg caacgggacg tgacttacgt
     5401 gcgcaacatg agcaccacct tcaccacggc catgggacac aacaaggtgg tcatcgatgc
     5461 gaccaagccc ctcgaccgcg ccttccccac tcgcttccgg gtgcctcccg ccgcgatgga
     5521 tggcgttcgt ctggaggatt ggattgacga ttggaccgga aggagcgtgg actgaatgga
     5581 taaggtgttc tgggtcacct gcccggactg tgcagggcgc ttcttctgcg atatggagtt
     5641 gcgccgcagc ggccacaagc tgatctgtcc gttctgtcag aacgagttcc tcgacgagga
     5701 gagccccgag atcgaggagt agagaccggg cgctaccgcg ggtgcatggc ccggaacgtt
     5761 tcgccggcgg tcagcacggt aatctatggg cgaaacgaga aaccaaccgg tcgcttgacg
     5821 agcgtcaccg tctcaacgtc gccagggcct ctcgtgccca ggaagggttc ttgagctggg
     5881 cccgccctac gcagacggca tcgaggaccc cgtgacgtac caggtcgtcg gcgtaggcgg
     5941 gggcggtgat gccccccacg gccgcaaccg ggatggcgac cgcccgcttg accgcggcac
     6001 ccgcctccac gaagtagccg ggttcggtgc ggtcggtcgg ccttgaaccg cagagtccgc
     6061 cgctgcagtc gatgaggtcc acgccccagt cctccagatg agcgccgatc gcggccgcgt
     6121 ccgccggggt tataccggag acgggcgcgt cgtccgcccc caaacggtac atgagcagtc
     6181 cgtgcccgag ttcgcgccgg acggcttcga gcgtctctct gatcagacgg agccggccct
     6241 gcaggtctcc accgtaggca tcgtcgcgat ggttggtgta ggggggcatg aactggtcga
     6301 gcaggtagcc gtgggctccg tgcacctcca ctccgtcgaa cctcgccgcc cgcgcccggc
     6361 gcgcggcggc cgcgaacaac tcggggatcg cttcgatctc ctcgattccc atcgggcggg
     6421 gctcctcctt gtcgccgggg acgacgacgc ccgaagggct gacggccgtg gcgccggtcg
     6481 cttcgcgggg actgcgggct ccggcatggg tgatctggat caccgcagcg gcgccgccct
     6541 catggatggc gtcggcgatg cgggcgagtc ccgccacgtg gtcgtcgctc cagatgccca
     6601 gctgatggcg gtcgacgcgg cctgtgaggg tcacgtaggc gtgctccgcc gccaccatgc
     6661 ccggacctcc ggccgcgtgc cgagtgtagt ggtcgacgag cgtatcggtg acgtaaccgt
     6721 gatcgtcggc cttgttgttg gccatgggcg gcatgatgat gcggttgcgg aggtgcggcc
     6781 cacgctccga tcagtctctg gtcagttcgt gccgaacaca ccggtcagtc tgcgccgaac
     6841 acgccggtca gctacggccg ataccggtgg tcagttcggc cgaaatacgc accatccgct
     6901 ctgggaaggc ggccaccagg ggcaggtgct ccgcgtgact cacccgcatc tcgtcgagca
     6961 gggacctcgc gtcgttcatg gctcacgtcc tcgacactcc gttgtactta tactatgata
     7021 caa
//
LOCUS       DASRMJ010000084         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_119062, whole genome shotgun sequence.
ACCESSION   DASRMJ010000084 DASRMJ010000000
VERSION     DASRMJ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119062"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1170
                     /locus_tag="VFD74_02455"
     CDS             220..1170
                     /locus_tag="VFD74_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZK48448.1"
                     /translation="MTSERQARALSGWIPLPFTIALYVAAFWLMLASGTGGWMLLLEL
                     GLLALAVLSSFGYFTLQPNEARLLILFGHYQGTTRQGGFLWGNPFFSNGPGGQARTFG
                     RLREGSGGGQDTRGLRRYKLSLRARTLNGDKLKVNDRAGNPVEIAAVVVWHVRDTARA
                     VFDVDDFEGYVRMQSETALRHLATAYAYDHGEGGESEITLRGNVDEVSQALKVELQER
                     LGKAGVEVDESRLTHLAYAPEIAQAMLRRQQAEAVIAARQKIVEGAVGMVDRALYLLG
                     EKKLVSLDEERKAAMVSNLMVVLCGESQVNPIVNAGTLYQ"
     gene            1217..1447
                     /locus_tag="VFD74_02460"
     CDS             1217..1447
                     /locus_tag="VFD74_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006722365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48449.1"
                     /translation="MARKQFPLRIDPELWQELEKWAADDLRSVNAQVEWLLREVVRKH
                     RGSRPRRDQDQAGRLPAEECECSAPPISSHQL"
     gene            complement(1430..1840)
                     /locus_tag="VFD74_02465"
     CDS             complement(1430..1840)
                     /locus_tag="VFD74_02465"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZK48450.1"
                     /translation="MGYEFSVRGRAKETERALEEACIGRFGTLRPEGATPVVRSDNGL
                     IFQSRRFRAALRDYRLRQEFITPYTPEQNGMIERFFRSLKEECVWQHLFVDFTEARRA
                     IREWIRWYNEERPHQSLEYLSPAGYRARQLQLVA"
     gene            complement(1995..2303)
                     /locus_tag="VFD74_02470"
     CDS             complement(1995..2303)
                     /locus_tag="VFD74_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HZK48451.1"
                     /translation="MLASFSVAPSGVGEGLGEFVAGMMPLIEASGLPFKLGAMQTTIE
                     GDEAEVMQLIMSCHRYMREKAPRVVTHIAIDDRAGATGRLEGKVQDVERILGRELRHE
                     "
BASE COUNT          446 a          764 c          768 g          438 t
ORIGIN      
        1 gcgctaatcg aatcggtatg gaggagtaga tgcttgagta cgaaatgccg aacaccgcgg
       61 ctgcggccgt tgtctggaag tgtttctctc aacgttccga agtcacagtt cgggaaggtt
      121 cgaccgcaca ccagtttgga gtagcggttt ctaggagata tcatggggat atctacaacg
      181 gcggtatcgg cgagcgccaa ctagagcgga ggaatcatca tgacgagtga acgccaagcc
      241 cgggcgctga gcggctggat cccgctgccg ttcaccatcg cgctgtacgt cgccgcgttt
      301 tggctcatgc tcgcctcggg aacgggtggc tggatgcttc tgcttgaact cggcctgctg
      361 gcgctcgcgg ttctctcatc cttcggctac ttcaccctgc agcccaacga ggcacgcctg
      421 ctcatcctct tcggccacta ccagggcacg actcgccagg gcggctttct gtggggcaac
      481 ccgttcttct cgaacggccc gggcgggcaa gccagaacgt tcggcaggtt gcgggaaggc
      541 tccggcgggg ggcaggacac acgcggtctg cgccggtaca agctttcgct gcgggcgcgt
      601 accctgaacg gcgacaagct caaagtcaat gaccgcgctg gcaaccccgt tgagatcgcc
      661 gcggtcgttg tgtggcatgt gcgggacacg gcgcgcgcgg tcttcgacgt cgacgacttc
      721 gagggttatg tgaggatgca gagcgagacc gcgctccgtc acctggccac cgcttacgcc
      781 tacgatcacg gagagggcgg cgagagcgag atcacgctgc gcggcaacgt cgacgaggtc
      841 tcccaggctc tcaaggtgga gctccaggag cgcctgggca aggcgggagt agaagtcgac
      901 gagtcgcgcc tcacccatct cgcctatgca cccgagatcg cccaggcgat gctccgccgc
      961 cagcaggccg aggcggtcat cgccgcccgc cagaagatcg tcgagggtgc ggtgggcatg
     1021 gtcgaccggg cgctctacct gctaggcgaa aagaagctgg tctcgctcga tgaggaacgc
     1081 aaagcggcca tggtgagcaa cttgatggtc gtgctctgcg gcgagtcaca ggtcaatccg
     1141 atcgtcaacg ccgggacact gtatcaatga gagcgcgggc acaccgagtg cgaactgcca
     1201 cagcgaaggg cgcctgatgg cacgcaagca gtttcccctg cgtatcgatc ccgagctgtg
     1261 gcaggagctc gagaaatggg ccgccgacga cctgcgctcg gtcaacgccc aggtcgagtg
     1321 gttgttgcgc gaggtagtgc gtaagcaccg gggcagtcgc cccaggcgag atcaagatca
     1381 agccggccgc cttcccgcag aggagtgcga gtgcagtgct cccccgattt caagccacca
     1441 gttgtagttg tcgagcccgg tacccggccg ggctcagata ctcaagactc tgatgcggtc
     1501 gctcttcgtt gtaccagcgg atccactccc tgatcgcccg acgtgcctcg gtgaagtcga
     1561 cgaacagatg ctgccagacg cactcctcct tgagactccg gaagaagcgt tcaatcatgc
     1621 cgttctgttc cggggtgtag ggggtgatga actcctgacg gaggcggtag tcccgaagcg
     1681 cggcccggaa gcgacgactt tggaagatga gtccgttgtc gcttctcaca acgggggtcg
     1741 ctccctcagg acggagcgtc ccgaagcgtc cgatgcaggc ttcttccaga gcgcgctctg
     1801 tttctttggc gcggccgcga acggagaact cataccccac gatctcgtaa tggtgctagc
     1861 cccacactcc ggaactggcc tcggctttgc gctcgcacgg cggattctcg tcgggccggg
     1921 tttagaccgc ggcggaagca cccgtgcagg agcctcaggc cagcccgtag cacgccgatg
     1981 aacccgccag gcacctactc gtggcgaagc tctctcccca ggatcctctc cacgtcttgc
     2041 accttgccct cgaggcggcc ggtggctccg gcgcggtcgt cgatggcgat gtgtgtcacc
     2101 acccgcggcg ccttttcccg catgtaacgg tggcagctca tgatgagctg catgacctcg
     2161 gcctcgtcgc cctcgatcgt ggtctgcatg gcgcccagct tgaagggaag cccggaggct
     2221 tcgatcagcg gcatcatccc cgctacaaac tcgcccagtc cttctccaac accagaggga
     2281 gcaacagaga aactggccag catgatggac ctccagtcct ctcggaatga ttccgcctac
     2341 ggcgcatccg ccgcgcggcc ctatcgggct cagtgaacca cgtctaggtc agggcgtcca
     2401 ccaaatccag attacg
//
LOCUS       DASRMJ010000085         8217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_186844, whole genome shotgun sequence.
ACCESSION   DASRMJ010000085 DASRMJ010000000
VERSION     DASRMJ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186844"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VFD74_02475"
     CDS             <1..910
                     /locus_tag="VFD74_02475"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HZK48452.1"
                     /translation="ANSLAGVLAGARQVECTINGIGERAGNAALEEIVMALKVRKDIM
                     PFATNINTTLLSKASKLVSNVTGFPVQYNKAIVGKNAFAHESGIHQDGVLKERTTFEI
                     MHAKDIGLGDSDIVLGKHSGRHALKSRLAELGIHLSGDELDEAFRRFKDVADKKKQVT
                     AIDLEALATEEIREREDLYSLTRFYVSAGSEIIPTSQVQVVREGAECHGKAFSGGSVE
                     SMFLAIDDAVGISGRLQDYRVRSISSGKDSLAEVRVVVEVEGKTFAGQAVSIDVLEAS
                     AKAYMRALNNVAQQREASGANPERVF"
     gene            1100..3823
                     /gene="acnA"
                     /locus_tag="VFD74_02480"
     CDS             1100..3823
                     /gene="acnA"
                     /locus_tag="VFD74_02480"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007142885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZK48453.1"
                     /translation="MSRGPEGAHSEGAPAGEPPRIADIRAIETRGFGWVDRLPYSIRV
                     LLENVLRHEGNGVTTEEHVRAVAAWQPNADERAEIPFMPSRVLLQDFTGVPCAVDLAA
                     LRSEVRRKGGEAGVVSPQVPVDLVIDHSVQVDHAGSPEAYELNVALEIERNRERYSLL
                     AWAQREFDDFTVVPTGTGIVHQINLEHLAAVVHSRTIDGESWAFPDTVVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAVMLGQPYFMLLPDVVGVRLAGELPLGTTATDLVLTITRTLR
                     EVGVVGRFVEFFGPGLARLTVADRATIANMAPEYGATCGFFPVDQRTIDYLVASGREP
                     AHAESVREYCRRQGLFREDGGAEPEYSRVVDFDLAQVRPSLAGPRRPQDLLPLGEVAR
                     SFRGSLAALAASAGRADVDVCSVEVDLPDGSSDRTFTLSDGAVVIAAIISCTNTSNPS
                     VMLAAALLARNAVQRGLRVPPWVKTSTAPGSRVVTSYLERSGLLPYLEALGFHIVGYG
                     CTTCIGNSGPLAAPVAAAIESGSLCTASVLSGNRNFEARVHPLVRSNYLASPPLVVAY
                     ALAGSVAVDLTQEPLGHDAAGIPVYLVDIWPSEEEVAQVVAGSVSPEQFKDRYADVTT
                     GDAAWQALEPPAGDLYAWDPDSTYVREPPFVVDLQAGPTPVSDIAGARILGIFGDSVT
                     TDHISPAGAIAAESPAGRYLMEKGVEPADFNTYGSRRGNHEVLVRGTFANVRIRNLML
                     DAKTGGWTIHVPSGETLTIFEAADRYRSEGTSLVVLAGKEYGTGSSRDWAAKGPALLG
                     VRAVIAESFERIHRSNLVGMGILPLQFESGQNAATLGLTGRETIDITGIAGGDLVPGG
                     RAHVRAAAPGHEVSFDAIVRLDSPVDAEYYRHGGILPRILRLMMERGRETS"
     gene            3810..4145
                     /locus_tag="VFD74_02485"
     CDS             3810..4145
                     /locus_tag="VFD74_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48454.1"
                     /translation="MRHPEVRLLHTAGCSPDNTEAARAALEVALVEAGLPAGGYRIVL
                     IRTEEEAVEQGFVGGPAILVDGVDVDPAVRGMKVGGLGCRAYFTADGISGAPPVEMIE
                     AALRGAMAE"
     gene            complement(4311..5006)
                     /locus_tag="VFD74_02490"
     CDS             complement(4311..5006)
                     /locus_tag="VFD74_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZK48455.1"
                     /translation="MTFSTRLVDRIPRVEGVKTFRFERIKQYVFTAGQWFVLTIPEGD
                     GYLTKHFTHSSSPTEGYLDFTTRLTGSQYKNALDALPVGTEVEIEGPFGEFTLRGEAK
                     RVAFITGGIGVTPIRSILRLMADTGDHREVALLYGNKDYDSIAFRLELESFSAQLPNL
                     KVAHVLSDPQPDWEGHRGFIRNEILDAELGNDAGWTYYVSGPPAMVAAMTELLTGRGV
                     SRQEMVLENFAGY"
     gene            5121..6326
                     /locus_tag="VFD74_02495"
     CDS             5121..6326
                     /locus_tag="VFD74_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZK48456.1"
                     /translation="MPTEPRMASRKIVGLAACASYERDVVIAALRKSLEPLGGMQAFV
                     APGERIAIKPNLLLAAHPDSAVTTHPAVVEAVAALVVECGAHPVVVESPGAGIPYSMA
                     TLRRVYRRTGMLELSERTGLELSFDTSSSRAVTHPEGRVTNRVDVLVPILEADGVISL
                     PKLKTHYFMTYTGAVKNLFGVVPGLAKPGYHARLADARRFADLLLDISLLVAPRLSVM
                     DAVLAMEGDGPGLGGSPRMIGALLAGADPVALDLAACRLVGLPPERVPTLTAARDRGM
                     WSGRFDDVHLIGDALESLQVSDFRIPDRSADEVGLGRAAWLGGVLTPIMRDLFSARPR
                     PRAGRCTGCETCRRACPARAIEMRDRLAVVDDGSCIRCYCCHELCPEAAVDLEFGRAA
                     RLVRTLGLR"
     gene            6323..7369
                     /locus_tag="VFD74_02500"
     CDS             6323..7369
                     /locus_tag="VFD74_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZK48457.1"
                     /translation="MRMVRGLATEGGTDIGSIYDETSDPEVVVPGIVMVAFDIDGVIL
                     RGGTLLPGASRAVQDVLDRGLSLRFVTNNSTRHRSEVAAGLADLGLPVQADMVLTSAA
                     ATAAWLAARIPRGARVLVVGGAGLVRELYEAGLDAVPACAASAAAAAPAASAAPATTA
                     ATEPWFPGAAVPATAAEVPAPPPPAVALSPDVSPAAVVVGLDVGFDYRTLATAQRALL
                     AGALFVATNTDATFPVEGRLLPGAGTIVAAVATAAGRKPVVIGKPEPGLADALVAEAG
                     GLPGAILFVGDKLSTDVEMASRAGMRSALVLTGVTARWEVDAAEAPRPDFVLETLEEL
                     PAVLARLGAGPVLR"
     gene            7381..>8217
                     /locus_tag="VFD74_02505"
     CDS             7381..>8217
                     /locus_tag="VFD74_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZK48458.1"
                     /translation="MDGIWSDYTRRVIGLLLLAAVFFVVWAARGVLAPLVMALVLAFL
                     IEPLVGGLNRRLRVPRFLAAVAVLLVLLVLFLLAIAVLGPILVEQFGQVRFNVDDAVD
                     GALGWLRSVSESMRNLDVFGYEFDLNAYVDSFQQGLEPSGLVGFLPSGQQLFGSLDNI
                     LSTTAGTLAGFASTLGRLLLTLFLTLIYSLYLVNDGPRLARAATALIPEQHKGELQEL
                     RCSVERVWRAFFRGQLLMVTIFGVMVGGSMWALGLPSALIVGIMAGVMDLVPTLGALV
                     AGG"
BASE COUNT         1214 a         2669 c         2887 g         1447 t
ORIGIN      
        1 ggccaattcg ctggccggcg tgctggcggg ggcgcggcag gtggaatgca ccatcaacgg
       61 catcggtgag cgcgctggca atgccgcgct ggaagagatc gtgatggcgt tgaaagtgag
      121 gaaggacatc atgcccttcg caaccaatat caacaccacc ttgctgtcga aggcctccaa
      181 gctggtatcg aacgtcaccg gctttccggt ccagtacaac aaggccatcg tgggcaagaa
      241 cgccttcgcg cacgagtcgg gcatccacca ggacggcgtg ctcaaggagc gcaccacctt
      301 cgagatcatg cacgcgaagg atatcggtct gggcgactcc gacatcgtcc tgggcaagca
      361 ctccgggcgt cacgccctca agtcgcggtt ggccgaactc ggaatccacc tctcgggcga
      421 cgagctcgac gaggccttcc ggcgcttcaa ggatgtggcc gacaagaaga agcaggtgac
      481 agccatcgac ctggaggcgt tggccaccga ggagatccgc gaacgcgagg atctctacag
      541 tctcacccgc ttctacgtct ccgccggctc ggagatcatc cccaccagtc aggtgcaggt
      601 ggtgcgagag ggcgctgagt gtcacgggaa ggccttctcc gggggatcgg tggagtcgat
      661 gttcttggcc atcgacgacg ccgtggggat ctccggcagg ctccaggact accgcgtgcg
      721 ctctatcagc tcaggcaagg actcgctggc cgaggtgcgc gtggtcgtcg aagtggaggg
      781 gaagacgttc gcgggccagg cggtttccat cgacgtgctg gaagcctctg cgaaggccta
      841 catgcgggct ctcaacaacg tggcccagca gcgggaagcg agcggcgcga acccggagcg
      901 tgtgttctag ttcttcagcg gcgcatacag gtctgcctgc cggcgggaga ggtccggttc
      961 ggtcgctggg tcggcgcctg gtcaccggat ggcaagatcc ggcgatccac cacgtctgct
     1021 accatgaagt cccgtttgta gcacgctcgc tggctgccgt gcgggtggtg cccggcgtcg
     1081 atgattggaa ggggagagga tgagtcgagg tcccgagggt gcccactcag agggtgcacc
     1141 ggcaggcgaa ccgcctcgga tcgccgacat ccgcgcgatc gagacccggg gattcgggtg
     1201 ggtagaccgg ctcccctaca gcatccgtgt gctgctcgag aacgtcctgc gccatgaggg
     1261 caatggggtc accaccgagg agcacgtgcg cgcggtggcc gcctggcagc cgaacgcgga
     1321 cgagcgggcc gagatcccgt tcatgccttc gcgcgtgttg ctgcaggact tcaccggcgt
     1381 gccctgtgcg gtggacctgg ccgcgctacg ctccgaggtg cgccgcaagg gtggggaagc
     1441 gggagtcgtg agccctcagg tccccgtcga cctggtgatc gatcactcgg tgcaggtaga
     1501 ccacgccggc agtcccgagg cctacgagct caacgtcgcc ctcgagatcg agcgcaaccg
     1561 tgagcgctat tcgctcctcg cttgggcgca acgcgagttc gacgacttca ccgtggtgcc
     1621 cacgggcacc ggcatcgtgc accagatcaa cttggagcac ctcgcggccg tggtccactc
     1681 gcgcaccatc gatggcgagt cgtgggcctt ccccgacacc gtggtgggaa cggactctca
     1741 caccaccatg atcaacggct tgggtgtgct gggctggggg gtgggcggca tcgaagccga
     1801 ggcggtgatg ctcggtcagc cctacttcat gctgttgccc gacgtggtgg gggtgcgttt
     1861 ggccggcgaa ctgcccctcg gcaccactgc caccgacctg gtcctcacca tcacgcggac
     1921 cctgcgtgaa gtgggcgtgg tcggccggtt cgtggagttc ttcggacccg gccttgcgcg
     1981 cctgaccgtg gctgatcggg cgactatcgc caacatggct cccgagtacg gcgccacctg
     2041 cggattcttc cccgtcgacc agcgcaccat cgactatctg gtggccagcg gccgagagcc
     2101 ggcgcatgcg gagtcggtgc gcgagtactg tcgccggcag ggactgttcc gtgaagacgg
     2161 cggggctgaa ccggaatatt cgcgggtggt ggatttcgac ctggcccagg tccggccgag
     2221 cctcgccggc ccgcgtcggc cgcaagatct cctgccgctg ggcgaggtcg ctcggagctt
     2281 ccggggttcg ctggcggcgc tcgccgcaag cgcagggcgg gcggacgtcg acgtctgctc
     2341 ggtcgaggtc gacctccccg acgggtcttc cgatcgcacc ttcaccctga gcgacggtgc
     2401 ggtcgtgatc gccgccatca ttagttgcac caacacctcg aacccgtccg tcatgctggc
     2461 ggcggcgctc ttggcccgga acgccgtgca gcgggggctc cgagtgcctc cgtgggtcaa
     2521 gaccagcacc gctccaggtt cacgggtggt cacgagctac ctggagcgct cgggcctcct
     2581 cccctacttg gaggccttgg gctttcacat cgtcggctac ggttgcacca cctgcatcgg
     2641 caacagcggg ccgttggcgg cgcccgtggc ggccgccatc gagagcggat cactctgcac
     2701 ggcgtcggtg ctcagcggca atcgcaactt cgaggcgagg gtgcatccgc tcgtccgctc
     2761 gaactatctg gcgtcgcccc ccttggtggt ggcctacgcg ttggccggtt cggtggcggt
     2821 ggatcttacg caggagcctc tcggacacga cgccgccggg atccccgtgt acttggtgga
     2881 catctggcct tcggaggagg aggtggccca ggtggtggcc ggatcggtct cgcccgagca
     2941 gttcaaggac cggtatgcag acgtcaccac cggcgatgcc gcgtggcagg ctctggaacc
     3001 gcccgccggt gacctctacg cgtgggatcc cgactccacc tacgtgcggg agccgccctt
     3061 cgtcgtcgat cttcaagccg ggcccacacc cgtctcggat atcgcagggg ctcggatcct
     3121 gggcatcttc ggcgactccg tcaccaccga ccacatctcg cccgccggcg ccattgcggc
     3181 ggagtccccc gcgggtcgct acctgatgga gaagggcgtc gaaccggccg acttcaacac
     3241 ctacggttct cggcggggca accacgaggt cctcgtgcgc ggcaccttcg ccaacgtgcg
     3301 catccgcaac ctcatgctcg acgcgaagac gggcgggtgg acgatccacg ttccctcggg
     3361 ggagaccctg actatcttcg aggcggccga ccgctaccgc tcggagggca cgtccctggt
     3421 ggtgctggcg ggcaaggagt acggcacggg cagttcgcgc gactgggcgg ccaagggccc
     3481 ggcgttgctg ggagtgcggg ccgtgatcgc ggagagcttc gagcgcatcc accgcagtaa
     3541 cctggtgggg atggggatcc tccccctcca gttcgagtcc ggacagaacg ccgcgacttt
     3601 gggcctgacc gggcgcgaga cgatcgacat caccggcatc gccgggggag acctggttcc
     3661 cggcggccgc gcgcacgtgc gcgcggccgc cccagggcac gaggtgtcgt tcgacgccat
     3721 agtccgcctc gactccccgg tcgatgcgga gtactaccgt cacggaggca tcttgccgag
     3781 gatcctccgc ctcatgatgg agcgcggtcg tgagacatcc tgaagttcgc ctgttacata
     3841 ccgccggctg ttcgcccgac aacactgagg ctgcacgcgc cgctctcgag gtggcccttg
     3901 tcgaagcggg gctccctgcg ggcgggtacc gcatcgtcct catcaggact gaagaggaag
     3961 ccgtcgagca gggcttcgta gggggccccg ccatcttggt ggacggagtg gatgtggacc
     4021 ctgcggtccg cggcatgaag gtgggcggcc tgggctgtcg agcctacttc acggccgatg
     4081 ggatcagcgg ggctccccct gttgagatga tcgaggccgc gctgcgcggg gcgatggcgg
     4141 agtaactgcg cgcacgggga gaggaagtcc agcgggaccg acgttgcgca atatgataga
     4201 tgcaacgcac attgcagagc agcctcggga gcgcctgaaa cctacgtagc atccggatag
     4261 tctcctcgcg gggccggccc caccaggccc gcgatcgagc aggcgttcga tcaatacccc
     4321 gcgaagttct ccagcaccat ctcctgacgg ctcacgccgc gtccggtcaa gagttccgtc
     4381 atcgctgcaa ccatggccgg cgggccgctg acgtagtacg tccacccggc gtcgttgcca
     4441 agttcggcgt ccaggatctc gttccggatg aagccgcggt ggccttccca atcgggctga
     4501 gggtcggaaa gcacgtgcgc gaccttcagg ttcgggagtt gagcgctgaa actctcgagt
     4561 tccaagcgga aggcgatgga atcgtagtcc ttgttgccgt atagcagcgc cacctcacga
     4621 tggtcgccgg tgtcggccat gaggcggagg atactgcgga tgggagtgac gccgatgccc
     4681 ccggtgatga aggccacgcg cttggcctcg ccgcgaaggg tgaactcgcc gaacggcccc
     4741 tcgatctcca cttccgtccc taccgggagc gcgtcgagcg cgttcttgta ttgcgacccc
     4801 gtgaggcgcg tggtgaagtc gaggtagccc tcggtggggg agctggagtg agtgaagtgc
     4861 ttggtcaggt agccgtcgcc ctcggggatg gtcagcacga accactggcc cgccgtgaac
     4921 acatactgct tgatgcgttc gaatcggaag gtcttgacgc cctcgacacg cgggatcctg
     4981 tctacaagtc tggtgctgaa cgtcatactc ttcactgttg accctcctcg acggcgcttc
     5041 gctcggagtc cccgcatgtt cgccgaggat gcgtccgtgg tccacggtgg ttcttgtccg
     5101 accaggatac aatggaagtc atgcctacgg aacctcgcat ggcctcgcgg aagatagtgg
     5161 gtctcgcggc ctgcgcatcg tacgaacggg acgtggtcat cgcggcgctc cggaagagcc
     5221 tcgagccgct tggcgggatg caggcgttcg tggcaccggg ggagcgcatc gccatcaagc
     5281 ccaacctgct gctggcggcc cacccggact ccgcggtcac gacgcacccg gcggtcgtcg
     5341 aggcggtggc ggctctggtt gtcgagtgtg gggcacaccc tgtggtggtg gagagtcccg
     5401 gtgccgggat cccctactcc atggcgactc tccgtcgagt ctaccgtcgc acggggatgc
     5461 tggagctgtc cgagcgcacg ggtcttgaac tctcgttcga cacctcctcc tcccgggccg
     5521 tgacgcatcc ggagggccgg gtgaccaacc gcgtggacgt gctcgtgccc atcctggagg
     5581 cggacggggt catctctctg ccgaaactca agacccacta cttcatgacc tacacgggcg
     5641 cggtcaagaa cctcttcggg gtggtgccgg ggctcgccaa acccggctac cacgcgcggc
     5701 tggcggacgc ccgccggttc gccgacctgc tgctcgatat ctcacttcta gtggccccac
     5761 gtctctcggt gatggacgca gtgctggcga tggagggcga cggccccgga ctcggcggat
     5821 cgccgcgcat gatcggcgct cttctcgccg gcgccgaccc cgtggcgctg gacctggccg
     5881 cctgtcgact ggtcggcctg ccgcccgagc gggtgcccac gctgacggcc gcgcgcgacc
     5941 gcggcatgtg gtcgggccgg ttcgacgacg tgcacctgat cggcgacgct ctggagtcac
     6001 tgcaggtgag tgacttccgc atccccgacc gatccgcgga cgaggtgggg ctcgggcggg
     6061 cggcgtggct cggcggggtg ctgacgccaa tcatgcggga tctcttcagc gcacgcccgc
     6121 gcccccgggc cgggcgctgc acggggtgcg agacgtgtcg ccgggcgtgt cccgcacgag
     6181 ccatcgagat gcgagaccgg ttggcggttg tggatgacgg gtcctgcata cgctgctact
     6241 gttgccacga gctctgcccg gaagcggcgg tcgacctcga gttcggacgc gccgctcgcc
     6301 ttgtgcgtac gttggggctt cgatgaggat ggtccgtgga ctcgccacag agggaggaac
     6361 cgacataggc tcgatctacg acgagacgtc ggacccggag gtcgtagtgc cgggaatcgt
     6421 gatggtcgcc ttcgacatcg atggcgtgat cctgcgcggg ggcacgctcc tgccgggggc
     6481 gtcgcgggcg gtgcaggacg tgctcgaccg cggcctgtcg ctgcgcttcg tgacgaacaa
     6541 ctccaccagg catcggtctg aggtagccgc cggactcgcg gacctgggcc tgccggtgca
     6601 ggcggacatg gtgctcactt ccgcggcggc cacggcggcg tggttggcag cgcgcatccc
     6661 caggggcgcg agggtgctgg tggtgggcgg cgccggtctg gtgcgtgaac tctacgaggc
     6721 gggactcgat gccgtgcccg cgtgcgccgc gtcggcggct gcagccgccc ctgccgcatc
     6781 ggccgcgccg gctacaacgg cggccacgga gccgtggttc cccggagccg cggtaccggc
     6841 cacggccgcg gaggtgccgg cgcccccgcc gcccgccgtg gcgctgtccc cggatgtttc
     6901 gccggccgcg gtcgtggtgg gtctcgacgt gggtttcgac taccggactc tggcgacggc
     6961 gcagcgggcg ctcctggcgg gcgccctgtt cgtggccacc aataccgacg ccaccttccc
     7021 ggtcgagggc agacttcttc ccggggccgg gaccatagtg gcggcggtgg ccaccgccgc
     7081 gggcaggaag ccggttgtga tcgggaaacc cgaaccgggc ttggccgacg cgctggtggc
     7141 agaggccgga ggcctacccg gggccatcct cttcgtcgga gacaagttga gcaccgacgt
     7201 cgagatggcg tcacgggcgg gcatgcggtc cgctctcgtc cttacggggg tgactgcgcg
     7261 gtgggaagtc gacgcagcgg aggcgccccg ccccgacttc gtgctcgaga ccttggagga
     7321 actccccgcg gtgctcgctc gtttgggcgc ggggccggtt ctgcggtagt ctgagccatc
     7381 gtggacggaa tctggagcga ctacactcga agagtcatcg gcctgctgtt actggccgcg
     7441 gtcttcttcg tggtgtgggc ggctcggggc gtgctcgccc ccctggtgat ggcgctcgtc
     7501 cttgcgttcc tcatcgagcc gctcgttggg gggctcaaca gacgcctacg ggttccccgc
     7561 ttcctggctg cagtcgcggt cctgctggtg ctgctcgtac tcttccttct ggccatcgcg
     7621 gtgctcggtc cgatcctcgt ggagcagttc ggtcaggtgc gcttcaacgt ggacgacgcg
     7681 gtcgacggcg ccctcggatg gcttcgatcc gtgtctgagt ccatgcgcaa cctagatgtc
     7741 tttggttacg agttcgacct gaacgcgtat gtggactcgt tccagcaggg cctggagccc
     7801 tccggccttg tgggcttcct gccgtcgggc cagcagttgt tcggctcact ggacaacatc
     7861 ctgtccacga ccgcgggcac gttggcgggg ttcgcctcga ccctcgggcg tctgcttctc
     7921 accctgttcc tgactctcat ttacagcctc tacctcgtga acgacggtcc ccggctcgcg
     7981 cgggccgcca ccgccctgat cccagagcag cacaagggtg aactgcagga gttgcgctgc
     8041 tcggtggagc gggtgtggcg cgccttcttc agggggcagc tgctgatggt gaccatcttc
     8101 ggcgtcatgg tgggggggag catgtgggcg ctggggctgc ccagtgcgct tatcgtgggg
     8161 atcatggccg gcgtcatgga cctcgtgccc acgctcgggg cgttggtggc ggggggg
//
LOCUS       DASRMJ010000086        19746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_82634, whole genome shotgun sequence.
ACCESSION   DASRMJ010000086 DASRMJ010000000
VERSION     DASRMJ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 19746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19746
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82634"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /gene="rho"
                     /locus_tag="VFD74_02510"
     CDS             complement(<1..1200)
                     /gene="rho"
                     /locus_tag="VFD74_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZK48459.1"
                     /translation="MELTELEPRLLSDLHVVAAELGIKNFRKYSKQDLISKILSEQSV
                     DRGVQYRTGLLDVLPDGFGFLRTQGYTQSDSDIYVSLSQIRRFKLRRGDEINGQVRTP
                     KDNEKYHALLKIQTVNGLDPEQARHRPSFDQLTPLFADERLRLETTPNRIAPRLMDLI
                     CPVGKGQRGLIVSPPKAGKTTILKEIANSISENNPEVHLLVLLVDERPEEVTDMERSV
                     NGEVVSSTFDQPAENHLQVTELVLERVKRLVEVGRDVVVLLDSITRLARAYNLAAPAS
                     GRILSGGVDSAALYPPKKFFGAARNIEEGGSLTILATALVDTGSRMDEVIFEEFKGTG
                     NWEVHLSRQLANKRIFPAIDIEKSGTRREELLMEPAEAKMVWKLRSVLHALDPNAAIE
                     LLIGKIKD"
     gene            complement(1260..2234)
                     /gene="glpX"
                     /locus_tag="VFD74_02515"
     CDS             complement(1260..2234)
                     /gene="glpX"
                     /locus_tag="VFD74_02515"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HZK48460.1"
                     /translation="MKSQEGLRCLCNDFVHVTEVAALAAGRWMGRGDAAAADMAAIEA
                     MSGTLNKLPISGTVVIGEEAQGDAALLRTGDKVGVGGRLCDIAVDALEGRDIVARGQS
                     GAVSVVAATTAGKLMSMPNMYMQKLVVGPLAAGRVDIDGRVTDTLLTIAEIYGRSVEE
                     VTVIVLDRPRHDDLIAEIRRAGARIRLITDGDVTAGIAVAVDRTNDQLYIGIGGAAEG
                     VLTAVAMRCLGGEIQARLWPLSRREVESARDHGIDDIEMTLKTEDMVGGEVLFAATGV
                     TSGEFLDGVQYLGWGVRTHSMVMCNHCNEVRFVRTTHLNTDTRREIRL"
     gene            complement(2231..3187)
                     /locus_tag="VFD74_02520"
     CDS             complement(2231..3187)
                     /locus_tag="VFD74_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of 2-phospho-D-glyceroyl
                     phosphate from ATP and 2-phospho-D-glycerate; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HZK48461.1"
                     /translation="MTPGTLRGHSETPGIRIVSDGHGVPFSKGLLAQSLTATGLPPDR
                     AYLVAMEVEKRIRKAGGLGIAVDHLHALVGRALGEMEGSDYLARYEKWTRSGVMHKPA
                     IVLIGGATGVGKSTIASQLANRLGIVRLISTDSIRQVMRTFFSEQLMPAIHYSSFNAG
                     AAIRVPVGKNLDPHLVAFVEQVEMVSVGVKAVIDRALEEGTSMVVEGVHLVPGFLDST
                     TWGEAVVLPVTVAVSDAELHMSHFLVRERDTDGRRPFTRYVEHFDEIRRIQDFILSRA
                     RAEGTLVVDNVDIDDAVASTVDTLYELIDVAEDAAGVVEGPQ"
     gene            complement(3184..4590)
                     /locus_tag="VFD74_02525"
     CDS             complement(3184..4590)
                     /locus_tag="VFD74_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HZK48462.1"
                     /translation="MALIDGEHYPPVVVDALVQLGSRFEFLAAVFLGGSEKIGGGGGD
                     DGPEGTAREVYGLPVHFASDGDIVGVLGQVIDMYRPETIVDVSDEPVVGYRERFRLVS
                     HALARNVSYEGSDFRFSPPKLENLATSPSLSIIGTAKRVGKTALSGFAARTIQEDLSR
                     AGGIRTLDGGRGGVVIVPMGRGGPAAPEVIDGAGGALDAGDLLAWSRAGRHAASDHFE
                     DAALSRVTTVGCRRCGGGMAGAPFVSNVAEGARVANSLGPSLTIFEGSGAAIPPVATD
                     ARLLVVGAHQPLDYVAGYLGTYRVLLSDAVVVTMAEQPLAEVEQVRRMVAEIESIKQG
                     IPVIPVVFRPQPLGDVAGRKVALFSTAPSVQEGLLRRHLEETYGCRVVAFSANLSDRG
                     RLRDDLAKAETHSAEVFLTEIKAAAIDVVAEEAEARGVQTVFMDNIPVEVEPSRRGEM
                     AELCRELARLAVDRFEGR"
     gene            complement(4632..5432)
                     /locus_tag="VFD74_02530"
     CDS             complement(4632..5432)
                     /locus_tag="VFD74_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZK48463.1"
                     /translation="MSKTRAMRIVHFSDIHCGSQYFVPNLLDRTLVEINDLAPEMVVL
                     TGDLTNMGYRQEFREAREYLDRLECRDVIVVPGNHDSRNVGYMHFERLFSSRDSEISK
                     GGVTVLGIDSTEPDLDYGRVGRHRYAWIKESYAQHPDDFKIFALHHHLLPVPGTGRER
                     NIVHDAGDVLEVLLASGVDLVLSGHKHVPYAWRLENMFVVNTGTVSTLRLRGNTKPCY
                     NVVQIAGERVTITRNYPFHGGEVIVEFDSEATTYVRHDFHLDRVVTDA"
     gene            complement(5482..6429)
                     /gene="sppA"
                     /locus_tag="VFD74_02535"
     CDS             complement(5482..6429)
                     /gene="sppA"
                     /locus_tag="VFD74_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZK48464.1"
                     /translation="MSRGWKIALGAMLAVVIGYAVLLAALLSGGGSSSAAWGTGSGTI
                     GVIRLDGVISAEGSGSLFSSGGIDPIGAVDLLREADADSGIDAVVLRVNSPGGSPAAS
                     WEIYQAVREMSKPVVVSVADIAASGAYYVASAADEIVAAPSSQVGSIGVILTAQDLEG
                     LFEKLGVRYTVLTKGEFKDIGSAAREMTAEEKRVLQAQIDMIYEQFIADVAAGRPGLD
                     ADQVRELATGLTYPGEQALELGLIDTIGSFSDALDAAAAAAGIAADDYGVEYLEPTAG
                     GDLLSLLLGYRSEALRQVGLGLADGLRQSLTGGSRLRLE"
     gene            complement(6478..7761)
                     /locus_tag="VFD74_02540"
     CDS             complement(6478..7761)
                     /locus_tag="VFD74_02540"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZK48465.1"
                     /translation="MEEGEERKVRVGLLGFGTIGSSVYGLIEKERPAILEATGVDLRV
                     SRILDIDTSKWGVDAPASLFTADFNDVVGDEEIDIVVELIGGIDRAFEFVSESLRRGK
                     EVVTANKQLIANRGAALFDLAREEGRQLRFEASVGGAIPVIRVMREGMIAAGLHSVYG
                     IVNGTTNYILTAMYEGEGGYADTLARAQELGYAEADPSADVGGGDAAAKMAILASIAY
                     RSRVTMADVTYEGIVDVTLEDVRYAKDFGFVIKLVGAARLIDGKISVRVHPALVPRDH
                     LLASINGSINAVYLQGHAIGEIMLTGPGAGGDPTATAVVGDVMTIAGTQAAGFLQNCS
                     CYKHLEFLSDDLVESAFFIRMQVEDRAGVLAQISSVFGAHQVSIESMIQKGQGDRAEL
                     VFITYPSLEKDFFRAIETIAGFACVRSRPMTIRVL"
     gene            complement(7771..9090)
                     /gene="lysA"
                     /locus_tag="VFD74_02545"
     CDS             complement(7771..9090)
                     /gene="lysA"
                     /locus_tag="VFD74_02545"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZK48466.1"
                     /translation="MTHTLIYPAHTRHNSAGRLEIDGCDVVGLAGEFGTPLVIYEEKT
                     LVDQCRRYMAAFRAHTDDFEVIYASKAFCCVSICQLIEREGLSLDVSSGGEYYTGLVA
                     GFPTQRMFFHGNNKTEAELRYALENGIGFVVVDSFDELALLERLAADRNEIQPVLLRI
                     TPGVEAHTHEFIQTGQLDSKFGFGLTEGHAEEAIRRTLEAGHLDLVGLHAHIGSQIFE
                     LEGFRRAIAVLVDLIKTMHDRVGFDCRYLNVGGGLGIRYTEEDAPLTIDAYVDCKVGG
                     VKQEMERVGLPMPRVLVEPGRSLVGKAGVTAYTVGTIKEIPGIRTYVSVDGGMSDNIR
                     PMLYGAKYEAMIADRAEAEAETRVTVAGKHCESSDILVKDALLAQPRVGDIVVIPATG
                     AYCYAMASNYNGNPRPAVVMVRDGEARVIIRRETYADLVADQLPLDG"
     gene            9267..9635
                     /locus_tag="VFD74_02550"
     CDS             9267..9635
                     /locus_tag="VFD74_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48467.1"
                     /translation="MSEEPPTFSYYRGTTPPRFVTQTERRAAEILDYYRVPWEYEPRT
                     FILEQGSSGNIVEAFTPDFYLPDLDLYIEVTQMKQSLVTTKNRKVRRLRELHPEVRIK
                     LLYKHDFIQLAKKFNLSLPS"
     gene            9713..10315
                     /gene="hpt"
                     /locus_tag="VFD74_02555"
     CDS             9713..10315
                     /gene="hpt"
                     /locus_tag="VFD74_02555"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZK48468.1"
                     /translation="MPTPSVSTARDTLPEGPAARTPPPDRALIGDVYLTAESIAARVA
                     ELGAEITTHYRDGDLVLVSVLKGAMFFMADLARAVDLPLEMDFMAISSYQQDHADPSH
                     RAIRFMKDLEQPLGGRDVIVVEDIVDTGLTLHYILRSLGLRSPRSLEVCTLLDRPYRR
                     IADVQVRYSGFTVPDDFFVGYGFDYRQLYRNLPYLAFLNI"
     gene            10318..11121
                     /locus_tag="VFD74_02560"
     CDS             10318..11121
                     /locus_tag="VFD74_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZK48469.1"
                     /translation="MVQTPPEKQIQTETLRFRSSAPADLRRGDRSGPVIAATLYLPAT
                     AHYREPLRPALIVGHGAGSRRGRHDSFCRVACAAGLVVLAIDFRGHGESTGELDGPAH
                     EDIIAAAALLRTHPLVDGRSICYRGSSMGGYYGLQAANDAGLAAAVLVCPASEQVLLA
                     GIARADDPDEVRSLSDRGLDLRLDVDGMREFLRSHRSVDAARRIDCPALLVHARGDDV
                     VPLRHTLDVAEALGGPAEITIVDEGDHSSIQGSQEMHEWTIRWLIQLLE"
     gene            complement(11185..11487)
                     /locus_tag="VFD74_02565"
     CDS             complement(11185..11487)
                     /locus_tag="VFD74_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZK48470.1"
                     /translation="MDDTSTSSEATKDDVTAVLNRLRRIEGQVRGLQRMIDEGKDCEQ
                     VLTQLSAVKSALDRVGIFLISHRMKECLATGSSAEVDEAAMEQAFEIFLKYVQCVR"
     gene            complement(11714..11789)
                     /locus_tag="VFD74_02570"
     tRNA            complement(11714..11789)
                     /locus_tag="VFD74_02570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11754..11756),aa:Gly,seq:ccc)
     gene            12249..13211
                     /locus_tag="VFD74_02575"
     CDS             12249..13211
                     /locus_tag="VFD74_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZK48471.1"
                     /translation="MKVIRVQTRGYLLVVVTATFWGFSGNLAKFLMKGDITPFALTQA
                     RSLVTAAVLLVYLLATNPSTLRLTRATAPWAVAFGLSLAATQVCYFSAIERLNVAAAI
                     LLEYLAPAIVVAYSWLFLRRAMTKTTLGSLISVLVGSALVVKAYDPAALSLSASGVAF
                     GLGAAIAFSAYILIGEHLQALGTGVATQLFYGFTVTTVLLMAIRPPWRAAPETFEPSN
                     LMLLAVVGILGTLLPFACFFASLRYIDAGRATIVSTLEPVVAGMVAFAWLGERFAGMQ
                     LVGAALIIGAIVALQRTDSGRGEIVMPLEVVGFSEETVDDLSRA"
     gene            13277..14722
                     /locus_tag="VFD74_02580"
     CDS             13277..14722
                     /locus_tag="VFD74_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZK48472.1"
                     /translation="MEQPAGTPRTTLRDIRGPLLMGGFLAVIQAGVVAGVLYAARPSL
                     TWAEIALLPLVVVSTVGIVVWVGMGRDTFKDISRQQAIQAEGILDIVIESLPHLRQGL
                     NPESAREVSYLIFAKLEFDAVSLTDTTRILSFVGEGENHHLPGTKIKTKATQEALDTR
                     QVQVVNNRWNIGCPYLDCSLHSAIIAPLIVRDQAVGALKLYYSRPKDIGELEEAVAMG
                     LARLLSSQLELAELDTQRQATLSAELRALQAQINPHFLFNSLNTIAMFCRTKPAEARS
                     LVLAFADFFRHTLRSPQDFISISEELEYVNNYLHLEKARFGPELVVNLSLSPEVLDLF
                     IPPLSIQPLVENAVKHGKGGVGRRLTIELAAWSEGDHLHVEVRDDGPGFDTDVYAEMR
                     NHGPSQAHGIGIFNVHRRLRAFYGPQFAFTLSSSKGTGSAVGFTLPMALCLPDDGPGA
                     DPTDRGLIGAVNGPDDGWVDDRSSRGALTGP"
     gene            14719..15114
                     /locus_tag="VFD74_02585"
     CDS             14719..15114
                     /locus_tag="VFD74_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HZK48473.1"
                     /translation="MSDRLPRNIPNGTGRERYGTWDLSSDRHPPEEQPEGTWESKVAD
                     HASEQSDEVIRRLIVRQRRLSIAATALLLGAVVGVVTISHLFPETMAVPVWRGFSPAF
                     LFMGVLIYPLTWIVAVLYVVISNRMDGLR"
     gene            15111..16742
                     /locus_tag="VFD74_02590"
     CDS             15111..16742
                     /locus_tag="VFD74_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HZK48474.1"
                     /translation="MNPVIADFETPVFALVFGVGLLALSVALAWNVRKLQKTAADYYL
                     AGRAVGILQNASAISGDYISAASFLGISGLTFLFGFDGLYYAVGFFLGFVVVLFFIAG
                     PLRKFGQYTIPDFVGGRFHTNEGRAVAVLCVLVISLFYTAPQMLGAAKILLILTHLPY
                     AVAVAMMSGSITLYVVLGGMRAATMIQIFQFWVLWCAIFLVALLVWQGGLPYDHVVTE
                     IQRLTTQETGAIRSQYMSPAGNRDLRESLSLLFALVCGTAGLPHILVRLYTNPDRARA
                     RWSVLLVLLFIGTFYLMTPYIGIAIHYLFLITASGAPAGTEGLSPQIMHWLTFDGQNL
                     AVPAAAMRFGGELALGIAVAGSLGAVLSTTAGLLVVMSGALGHDLYYSLYNPKASQRA
                     RVLVARGSTVVMGIVVFLLGLAVERMQIAVLVGLAFAISASTLFPVLILGLWWRRMTG
                     PGAIAGMVTGLGVSLFLIFGKSLLPVWLRFENPGGVSVAASFLAIVVASKVFGRVPDD
                     VDRFMALVHGTSEEQRIRRASRIKRRKVFAGLPRR"
     gene            complement(16803..17618)
                     /gene="mutM"
                     /locus_tag="VFD74_02595"
     CDS             complement(16803..17618)
                     /gene="mutM"
                     /locus_tag="VFD74_02595"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZK48475.1"
                     /translation="MPELPEVETVRCQLDAALPGREIVSVESVDPFILRDADAQDLPG
                     LIEGRAFGSVTRAGKFLVLPLQGDVYLTIHLGMTGQLLIAPRGAEISHVRFSGLLHGG
                     EDRLVFRDIRKFGRLHVTLGAPAARLSGLGPDAWIGDWDADFLRSRLNGRTAPLKAFL
                     LDQRHLAGIGNIYADEILYGARLSPLRPAGTLTRPETERLAREIRERLGEGVRLRGCS
                     ISDFVDTEGAPGSFQDALRAYGRHGLPCASCGSPLVRTVVGGRGAAFCRYCQR"
     gene            complement(17626..19329)
                     /gene="argS"
                     /locus_tag="VFD74_02600"
     CDS             complement(17626..19329)
                     /gene="argS"
                     /locus_tag="VFD74_02600"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZK48476.1"
                     /translation="MLESAVCRVVEEWVAENGWTGTPDLVVERPSDEGHGDYAVPYCL
                     QLAKIARRPPRALAEELRVRFLADPEVSALVERVDVAGPGFVNLTLSSAAFAGAVRAM
                     LVAGSDVGRGTRDKPLHILLEYVSVNPNGPLHVGHGRYAAYGNALARILAHSGHRVTT
                     EFYINDYGRQMEMFGRSVAARYAQAFDVDLAVPEDGYQGEYVKGIAAVITAEVGDRWV
                     EALARDGIDDADAPSVSYFRVRGYELVLKQMSTELQMFGVTFGRWFSETPLHTEGRVQ
                     ATIDRLLETGDAYRKDDAIWLRTTQYGDDKDRVLIRSNERPTYFAADIAYHQDKILRG
                     FDHLINIWGADHHGYIPRMKAAVEVLSHDPDMLEIIIGQMVNLLEEGELRQMSTRKGE
                     MVTLGELIEAIGVDAARFFLVMRSHDQTLDLDIGLARQQTQENPVFYVQYAHARVASI
                     LRNLPEDLAGRLGEGAELLAGVSEGAAVFSTTFERDLVKVLASFEDVVREAAHRRAPH
                     RIAGYAQEVAGRFHVFYKNCRVIGAGDDVAASRLALCLVSQWVIARCLYLLGVSAPES
                     M"
     gene            complement(19346..>19746)
                     /locus_tag="VFD74_02605"
     CDS             complement(19346..>19746)
                     /locus_tag="VFD74_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZK48477.1"
                     /translation="LKKVKKAGADHAISPYSIGGQKMAGLLLKPLVNDYLEVVAGGGE
                     IEFRVEEFALNDTCEIVGRSIAELEVRKRTGATILAVRHGSSGLFDTNPNPGLILGDG
                     DVIIAIGTPVDIANLEELFACRIPASGGGH"
BASE COUNT         3383 a         6765 c         6068 g         3530 t
ORIGIN      
        1 gtccttgatc ttgccgatca gcagctcgat tgcggcgttc ggatcgaggg catgcaggac
       61 cgaccgcagc ttccagacca tcttggcctc cgccggctcc atgagcaact cttcgcgacg
      121 cgtgcccgac ttctcgatgt cgattgccgg gaagatccgc ttgttcgcca gctggcggct
      181 aagatggacc tcccagttgc cggtgccctt gaactcttcg aagatgacct cgtccatacg
      241 ggagcccgtg tccacgagcg ccgtcgccag tatggtgagc gaaccgcctt cttcgatgtt
      301 ccgagcggcc ccgaagaact tcttcggggg gtagagtgcg gcggaatcca cgccgcccga
      361 tagaatacgg ccggaggccg gggcggccag gttgtaggcc cgtgcaagac gcgtgatcga
      421 gtcgagcagc acgacgacgt ccctgcccac ctccacgagg cgcttgaccc gctccaagac
      481 cagctcggtc acctgcaggt ggttctccgc gggctggtcg aaggtggagc tgacgacctc
      541 gccgttcacc gacctctcca tgtcggtgac ttcctccggc cgctcatcga cgagcagcac
      601 gagcaggtgc acctctgggt tgttctcgga gatcgagttg gcaatctcct tcaggatggt
      661 ggtcttgccg gccttcggcg gagacacgat caacccgcgc tgacccttgc ccacggggca
      721 gatgaggtcc atgaggcgag gggcgatacg gttgggagtg gtctcgagac gcagcctctc
      781 gtccgcgaag agcggggtga gctgatcgaa gctgggacgg tgacgcgcct gctcggggtc
      841 gagcccgttg accgtctgga tcttgagcaa ggcgtgatac ttctcgttgt ccttgggcgt
      901 gcgcacctgg ccgttgatct catcgccgcg gcgcagcttg aagcgccgga tctgcgagag
      961 cgacacgtag atatcggagt ccgactgtgt gtacccctgg gtgcgcagaa acccgaaacc
     1021 gtcgggcagc acgtccaaga ggccggtgcg atactgcaca ccgcggtcca cggattgttc
     1081 actcaggatc ttcgagatca gatcctgctt cgagtacttc ctgaagttct tgatcccgag
     1141 ctccgccgcc accacgtgca ggtcggatag gagcctcggt tcgagctctg tcagctccat
     1201 tcgccctccc tggtcacaag ctcggtcatc tcatcgatca tctttcgttc tctccattcc
     1261 tagagccgga tctctcggcg cgtgtcggtg ttcagatgtg tggtcctgac gaagcggacc
     1321 tcgttgcagt ggttgcacat gaccatggag tgagtgcgga cgccccagcc gaggtactgc
     1381 accccgtcca agaactcacc gctcgtgacc ccggtagccg cgaacaggac ctcaccaccc
     1441 accatgtcct cagtcttcaa ggtcatctcg atgtcatcga tgccgtgatc gcgggcggac
     1501 tccacctcgc ggcgcgacaa gggccaaagg cgcgcctgga tctcgccacc gaggcagcgc
     1561 atggccacgg ccgtcagcac cccttcggcc gcccccccga taccgatata cagttggtcg
     1621 ttcgtgcggt cgaccgccac ggcgatcccg gctgtcacgt caccatcggt gatcaacctg
     1681 atgcgggcgc cggcgcgacg gatctctgct atcaggtcgt cgtggcgcgg ccggtcgagg
     1741 acgatcacgg tgacctcctc cacgctgcga ccgtagatct cagcgatggt gagcaaggtg
     1801 tccgtgacgc gcccgtcgat atccacgcgg cctgcggcca aggggccgac gaccagcttc
     1861 tgcatataca tgttgggcat ggacatcaac ttgccggcgg tcgtggccgc cacgacactc
     1921 accgccccgg attgaccacg ggccacgatg tctcgccctt cgagggcgtc caccgcgatg
     1981 tcgcacagtc gcccgccaac tcccaccttg tcgcccgtgc gcaacagagc cgcatcgccc
     2041 tgggcctctt ctcctatcac cacggtcccg ctgatgggca gcttgttcag ggttcccgac
     2101 atcgcttcga tagccgccat gtcggccgcc gccgcgtctc cccgccccat ccatcgtccg
     2161 gcggccagcg cggcgacctc ggtcacgtga acgaagtcgt tgcacaagca ccgcaagccc
     2221 tcttggctct tcattgaggc ccctccacca cacccgccgc gtcctccgcg acgtcgatca
     2281 attcgtacag ggtgtctacg gtgctcgcga ccgcgtcgtc gatgtccacg ttgtccacca
     2341 cgagcgtgcc ttccgcccga gcgcgcgaca agatgaaatc ctggatgcgc cggatctcgt
     2401 cgaaatgctc gacgtagcgc gtgaagggtc gtctgccgtc ggtatccctc tccctcacca
     2461 ggaagtgact catgtgtagc tcggcgtcgc tcacggccac ggtcaccggc aggaccactg
     2521 cctcgcccca ggtggtcgag tcgaggaagc ccggcacgag atgcacaccc tccaccacca
     2581 tcgaggttcc ttcctccagc gcacggtcga tgactgcctt cacgcccacg gagaccattt
     2641 cgacctgctc cacgaaggcg accaggtgcg gatccaggtt cttccccacg ggaacacgga
     2701 tagcggcccc ggcgttgaac gaggagtagt ggatcgcggg catcagttgc tctgagaaga
     2761 acgtgcgcat gacctgccgt atggaatcgg tggagatgag ccggacgatg cccagcctgt
     2821 tggcgagttg actggcgatg gtgctcttgc ctacgcccgt ggcgccgccg atcaagacga
     2881 tggccggctt gtgcatgacg cccgacctcg tccacttctc gtagcgagcc agatagtcac
     2941 tgccctccat ctcgccgagc gctctgccca ccagcgcgtg aagatgatcc acggcgatgc
     3001 cgagcccgcc cgccttgcgg atccgcttct ccacctccat ggccaccagg taggcgcgat
     3061 cgggcggcag tccggtcgcg gtgagcgact gcgcgagcag tcccttggag aacgggactc
     3121 cgtggccgtc gcttactatg cggatgcccg gcgtctccga gtgccccctc aaggtcccgg
     3181 gagtcatcgc ccctcgaacc tgtccacggc cagtcgggcc aactcacgac acagttccgc
     3241 catctccccg cggcgggaag gttcgacctc cacagggatg ttgtccatga acaccgtctg
     3301 cactccgcgg gcttcagcct cctcagccac cacgtcgatc gcggcggcct tgatctctgt
     3361 caggaacact tcggcggagt gcgtctcggc cttcgcgaga tcgtcccgca gcctacctcg
     3421 gtcggagagg ttcgccgaga aggcgacgac ccggcacccg tacgtctctt ccaggtggcg
     3481 gcgaagcaga ccctcctgca ccgacggagc ggtggagaag agggcgactt tgcggcccgc
     3541 cacgtcgccg agcggttgcg gtcggaacac caccgggatc acgggtatcc cctgctttat
     3601 ggattcgatc tcagcgacca tccgcctcac ctgctcgacc tccgccagcg gctgctcagc
     3661 catcgtcaca accaccgcat cactcaacaa gaccctgtac gtgccgaggt accccgccac
     3721 gtagtcgagt ggttggtgcg cacccacgac gagcaaccgg gcatccgtgg ccacaggagg
     3781 gatcgcggct ccgctcccct cgaatatggt gagcgacgga ccaagagagt tcgcgacccg
     3841 agccccttcg gctacgttcg atacgaaagg cgcacctgcc atccctcccc cgcagcggcg
     3901 acaccccacg gtggtcacgc ggctgagcgc ggcgtcctcg aagtggtctg aagccgcatg
     3961 ccgccccgcc cgactccaag ccagaaggtc accggcatcg agggcgcccc cggctccgtc
     4021 gatcacttcg ggggctgcgg gtccgccgcg ccccatgggg acgatcacta ctccgccccg
     4081 gccgccatcg agcgtccgga tgccgcccgc tcgcgacaga tcttcttgga tggtacgcgc
     4141 cgcgaagcca ctgagggcgg tcttgcccac gcgcttcgcc gtcccgatga tcgacagcga
     4201 tggagacgtg gcaaggtttt cgagcttggg gggtgagaaa cggaaatcag acccttcgta
     4261 cgagacgttc cgcgccaacg catggctgac taagcggaac ctctcccgat agcctaccac
     4321 aggctcgtcg ctgacgtcaa cgatagtctc agggcggtac atatcgatca cttgccccag
     4381 gactccgacg atgtcgccgt ccgaggcgaa atgcacaggc aacccgtaca cctcccgcgc
     4441 cgtcccctcc ggaccgtcgt cgccgccgcc cccgccgatc ttctcgctcc caccgaggaa
     4501 gacggcggcg aggaactcga accgggaccc cagttggacc agcgcatcta ccacgaccgg
     4561 ggggtagtgc tccccgtcga tcagagccac cgcggagatc cgcgctccgc tgtcggccct
     4621 cccccccacg gtcatgcgtc cgtaacgacc cggtcgaggt ggaagtcgtg gcgcacgtag
     4681 gtggtcgctt cgctgtcgaa ctccacgatc acctcccctc cgtgaaaggg gtagttccgt
     4741 gtgatcgtga cgcgctcgcc ggcgatctgc acgacgttgt agcagggctt agtgttgccc
     4801 cgcaggcgca gggtcgacac cgtcccggtg ttgaccacga acatgttctc aagtcgccac
     4861 gcgtagggca cgtgcttgtg gccggatagc acgaggtcca cgcccgaggc gagaagcacc
     4921 tctaggacgt cgcccgcatc gtgcacaatg ttcctctcgc gccccgtccc aggcacgggg
     4981 agcaggtggt ggtgaagagc gaagatcttg aagtcgtccg gatgctgcgc gtagctctcc
     5041 ttgatccagg cgtacctatg ccgacccacc cgtccgtagt cgaggtcggg ttccgtggag
     5101 tcgataccca gcacggtcac gcctcccttc gagatctcag agtcgcggct ggagaagagc
     5161 cgttcgaagt gcatgtaccc gacattgcga ctgtcgtggt tgccgggcac cacgatgacg
     5221 tcccggcact cgaggcgatc gaggtactca cgggcctccc tgaactcctg acggtatccc
     5281 atgttggtga gatcaccggt gagcacgacc atctcgggag ccaggtcgtt gatctcgacc
     5341 agagtccgat cgagaaggtt cgggacaaaa tactgagagc cgcagtggat atcagagaag
     5401 tgcactatcc gcatcgcacg ggtcttcgac atgaaggatc ctctcgcttc tccagagccc
     5461 gaccccacgg ggagagggac tctattccag acgcagccgg ctcccgccgg tcagcgattg
     5521 tcgcaggccg tcggccagac cgagccccac ctgtctcaac gcctcgctcc tatacccaag
     5581 cagaagcgac aacaggtcac cgcccgcggt cggttcgagg tactcgaccc cgtagtcgtc
     5641 cgccgcgatg ccggccgccg ccgcagccgc atccaacgca tcggagaacg agcctatcgt
     5701 gtcgatgagg cccagttcga gggcttgttc cccgggatag gtgaggccgg tagcgagttc
     5761 tctcacctgg tcggcgtcga gcccgggacg accggccgcc acatcggcga tgaactgctc
     5821 gtagatcatg tcgatctgcg cctgcagcac gcgcttctcc tcggccgtca tctcgcgggc
     5881 agcgctcccg atgtccttga actcaccttt cgtaaggacc gtgtacctca cgcccagttt
     5941 ctcgaagagc ccttccaggt cctgcgcggt caagatcacg ccgatgctgc ccacctgcga
     6001 cgagggggcg gccacgatct cgtccgcagc gctcgccacg tagtaggccc ccgatgcggc
     6061 gatgtcggcc accgacacga ccacgggctt gctcatctcg cgcaccgctt ggtagatctc
     6121 ccacgatgcc gccggacttc cccctggact gttcacgcgc aacaccaccg cgtcgatgcc
     6181 ggagtcggcg tcggcttctc ggagcaggtc gacggcaccg atcgggtcga taccccccga
     6241 cgagaagaga gatccggagc cctcagccga gatgacgccg tccagccgga tgaccccgat
     6301 cgtgccggaa ccagtgcccc acgccgcgga ggacgatccg ccgccgctga gtagagcagc
     6361 caacagcacg gcatacccga tcacgaccgc cagcatcgct cccagagcta tcttccagcc
     6421 tcttgacacc cgttccacct ccagccgcgg cgcggggtcc gcgccaaatc ggtcgtccta
     6481 gagcacccgg atggtcatag ggcggcttcg cacgcacgcg aaaccggcta tcgtctcgat
     6541 cgcgcggaag aagtccttct cgagcgacgg gtaggtgatg aacaccagct cggcccgatc
     6601 gccctgaccc ttctggatca tgctctcgat agacacctgg tgcgccccga acaccgagga
     6661 gatctgcgcc aacactcctg cccggtcctc cacctgcatg cggatgaaga atgcggattc
     6721 cacaagatcg tccgacagga actccaggtg cttgtagcac gagcagttct gcaagaagcc
     6781 ggccgcctgc gtgccggcga tggtcatcac gtcgcccact acagcggtgg ccgtggggtc
     6841 gccgcctgcc ccgggcccgg tgagcatgat ctcgccgatg gcgtgcccct gaaggtagac
     6901 ggcgttgatc gaaccgttga tggaagcgag gaggtggtcg cgcgggacca gggccgggtg
     6961 cacccgcacg ctgatcttgc cgtcgatgag acgggcagcc ccgaccaact tgatgacgaa
     7021 cccgaagtcc ttcgcatacc gcacgtcctc gagcgtcacg tcgacgatgc cctcatacgt
     7081 gacgtcggcc atggtgaccc gcgaacggta ggcgatcgaa gccaggatgg ccatcttggc
     7141 cgcggcgtca ccgccgccca cgtccgcgct ggggtccgct tcggcatagc ccagttcttg
     7201 cgcacgggcc agggtgtcgg cgtagccgcc ctcgccctcg tacatcgcgg tgaggatgta
     7261 gttggtggtg ccgttgacga tcccgtagac cgagtgcagg ccggcggcga tcatgccctc
     7321 ccgcatcacc ctgatcaccg ggatggcgcc gcccacactc gcctcgaagc ggagctggcg
     7381 tccctcctcc cgcgccaggt cgaagagcgc ggcccccctg ttcgcgatca gctgcttgtt
     7441 ggcggtcacc acctccttgc cccggcgtag ggactccgac acgaactcga aggctcggtc
     7501 tatgccaccg atgagttcga ccacgatgtc gatctcctca tctccgacca cgtcgttgaa
     7561 gtcggcggtg aagagactcg caggggcgtc cacgccccac ttgctagtgt cgatgtccaa
     7621 gatccgagac acccgcagat ctaccccggt tgcctccaag atcgcagggc gctccttctc
     7681 gatgagaccg tagacgctcg acccgatggt cccgaacccc agcagaccta ctcggacctt
     7741 gcgctcctca ccttcctcca tcctgacccc ctacccgtcg agcggaagtt gatcggccac
     7801 gagatccgcg taggtctcgc gccggattat cacccgggcc tccccgtctc tgaccatgac
     7861 cacggcggga cgagggttgc cgttgtagtt cgaggccatc gcgtagcagt atgccccggt
     7921 ggcagggatc acgacgatat ccccgacacg cggctgggcc aagagcgcgt ctttgaccag
     7981 gatgtcactg ctctcgcagt gcttgcccgc aacagtcacg cgcgtctccg cctccgcctc
     8041 ggcgcggtcg gcgatcatag cttcgtactt cgcgccgtag agcatgggcc ggatattgtc
     8101 gctcatgccg ccgtcgacgc tcacataggt gcggatgccg gggatctcct tgatggtccc
     8161 caccgtgtac gccgtgacgc cggccttccc caccagggag cggcccggtt ccaccagcac
     8221 gcgcggcatg ggcagaccca cgcgctccat ctcctgcttc actccaccca ccttgcagtc
     8281 cacgtaggcg tcgatggtaa gcggcgcgtc ttcctcggtg tatctgatac ccagaccgcc
     8341 gcccacgttc agatagcggc aatcgaagcc cacccggtcg tgcatggtct tgatcaagtc
     8401 aacgaggacg gcgatcgcgc gccggaaccc ttcgagctcg aagatctggc tgccgatgtg
     8461 ggcatgcaga cccaccaggt cgaggtggcc tgcttcgaga gtccgcctga tcgcctcttc
     8521 cgcgtgtccc tcggtgagac cgaaaccgaa cttcgagtcg agttggcccg tctggatgaa
     8581 ctcgtgggtg tgagcttcca ccccgggggt gatgcggagc agcaccggct ggatctcgtt
     8641 ccgatcggcc gcgaggcgtt cgaggagagc gagctcgtcg aagctgtcga cgaccacgaa
     8701 gccgatgccg ttctcgagcg cgtagcggag ttcggcctca gtcttgttgt tgccgtggaa
     8761 gaacatgcgc tgggtaggga agcccgccac caagcccgtg tagtactcgc cgcccgagga
     8821 cacgtccaga gacaggccct ctcgctcgat cagctggcag atcgacacgc agcagaacgc
     8881 cttgctcgcg tagatgacct cgaagtcgtc ggtgtgggcg cgaaaggccg ccatgtagcg
     8941 ccggcactga tccacgagcg tcttctcctc gtagatcacc agaggagtgc cgaactcacc
     9001 ggccagcccc accacgtcgc aaccgtcgat ctcgagtcgg cccgcggagt tgtgccgggt
     9061 gtgcgccggg tagatgaggg tatgggtcat cgggtcttgc tcctgggggg tgcggaaggc
     9121 gaacatcctg ctcgcagtac ggtaccacac agtccattct cccgcgacgg gggaaaggag
     9181 gcgcgccgag cggtcccatg caccgagcgg tcccgtgcgc cgagtggtcc ggtcgagagc
     9241 ggggtctgat agaatcagat tccaggatga gcgaagagcc acccaccttc tcctactaca
     9301 gagggacgac accgccgagg ttcgtgaccc agacggagag acgagccgcg gagatcctcg
     9361 actactaccg cgtgccttgg gaatacgagc cgcgcacgtt catcctcgag cagggatcga
     9421 gcggcaacat cgtggaagca ttcactcccg acttctacct ccccgatctc gatctctaca
     9481 tagaggtcac gcagatgaag cagtcgctgg tcaccacaaa gaaccgcaaa gtacggcgtc
     9541 tgcgcgagct gcaccccgag gtccgcatca agctcctcta caagcacgac ttcatccagc
     9601 tggccaagaa gttcaaccta tcgttgccgt cgtgagcgac ttgcctgccg catctccaca
     9661 accgccggtc tcggcgcatc gagtgcccac gccgtcggtc tcggcgcatg ggatgccgac
     9721 gccgtcggtc tctacggctc gcgacactct gccggagggc ccggcggctc ggacaccgcc
     9781 gcccgatcgc gccctcatcg gcgacgtgta ccttacggcc gagagcatcg ccgcccgggt
     9841 ggccgagctc ggcgccgaga tcacgacgca ctaccgtgac ggagacctgg tcctggtgtc
     9901 ggtgctgaag ggggccatgt tcttcatggc cgacctcgct cgggccgtcg acctccctct
     9961 agagatggac ttcatggcca tctcctcata tcagcaggac cacgcggacc cgagccatcg
    10021 cgctatccgg ttcatgaagg acttggagca gccgctcggc ggtcgcgacg tcatcgtggt
    10081 cgaggatatc gtggacaccg gcctcacctt gcactacata cttcgttcgc tgggcctccg
    10141 ctcgcctcgg tcactggagg tgtgcaccct cctcgaccgt ccctacaggc gcatcgcgga
    10201 cgtccaggtc cgctactcgg gcttcactgt ccccgacgac ttcttcgtgg gctacggctt
    10261 cgactatcgg cagctctacc gcaacctgcc ctatctggcc ttcctcaaca tctgagagtg
    10321 gtccagacgc cgcccgagaa gcagatccag accgagactc tccgtttccg gagcagtgcg
    10381 cccgcagacc tcagacgcgg cgaccgttcc ggccctgtca tcgccgccac gctctacctg
    10441 ccggcgaccg ctcactaccg cgaacctcta cggcccgccc tcatcgtggg ccacggggcc
    10501 ggcagccgcc gcggccgcca tgactccttc tgccgggtcg cctgcgcggc cggtctggtg
    10561 gtgctcgcga tagacttccg cggccacggg gagagcacgg gtgagctgga tgggccggcg
    10621 cacgaagaca tcattgccgc cgccgcgctc ctgcgcaccc acccgttggt ggacggccgg
    10681 agcatctgct accggggttc gagcatggga ggctactacg gcctgcaggc ggcgaacgac
    10741 gccggcctcg cggcggccgt cctcgtgtgt ccggcgagcg agcaggtgtt gctcgccggc
    10801 atcgcccgag ccgacgaccc cgacgaggtc aggtcactct ccgaccgcgg gttggacctg
    10861 cgcctggatg tcgatggtat gcgcgaattc cttcgcagcc atcgcagcgt ggacgccgcc
    10921 cgccgcatcg actgcccggc gcttctggtt cacgctcgcg gagacgatgt ggttccgttg
    10981 cggcacaccc tcgacgtggc tgaggctctc ggcggtcccg cggagatcac gatcgtcgac
    11041 gagggggatc actcatccat ccagggatcc caggagatgc acgagtggac gatccgatgg
    11101 ctgattcagc tgctggagta gtccccggcc gctcgcccgg cggtcgctct cgtggagggt
    11161 tctccgacta acggtcccgc gcgttcagcg gacgcactgc acgtacttca agaagatctc
    11221 gaatgcctgc tccatggcgg cctcgtccac ttcggcgctg gatcccgtcg cgagacactc
    11281 cttcatacgg tgactaatca ggaagatccc cactcggtcc agtgccgact tcacggcaga
    11341 gagctgcgtg agcacctgct cacagtcctt gccctcatcg atcatccgct gcaggcccct
    11401 gacttgaccc tcgatgcgcc gcagccggtt gaggactgcg gtcacgtcat ccttggtcgc
    11461 ttcgctggac gtgctcgtat catccatgca gggccccttc cgttcgatgc gtcgttgccg
    11521 cggcactacc cttccagaag gggggcgaca ctcgacaccc gccctctggg actcgtgaag
    11581 tatacccctg cgggtaatcg cgacgcaaca cctcgtggag tgctcggaag tgcggctccg
    11641 gagcccgcgg tgcacgaggc ccggcgtacg cggcccagcg taggtggcaa gaggaatcgc
    11701 gcgaaagaag gagtggagcg gagtacgggg atcgaacccg cgacatccag cttgggaagc
    11761 tggcactcta ccagctgagt taactccgcc cgcgggtctc cttgtcgcgg ccagattgta
    11821 gcacagcacc aagctgcgct gcgtggcagg cgggactcta ttcggagcca tcgggcgcgc
    11881 ctcgccaagc caacgcacgc ccgcccggta cgctcacagc ggacgcccgg tacgctgaac
    11941 aacaccgcat tttgcgggaa cttcctggca tcgcggggct tgacaggaga gaagtcgggt
    12001 gtagaatagt atacgagtcc cgagagatca gacgcaccat ctggagactc ccacagatgg
    12061 cattgtggag gaaacgcgga agtaaatcgc cgcggagtac gatgaagcag tggaagtgga
    12121 agtcgatgaa ggaagggaag cgagttcact cgggactcgc tttttttctg ccgcgaccca
    12181 ggctcaagac gatcggcacc gatcgatcca cggcctcgta gccgggccca cctgacgcga
    12241 agaatggagt gaaggtcatc cgagtgcaga cacgcggcta cctcctggta gtcgtaaccg
    12301 cgactttctg gggcttctca ggaaacctgg ccaagttcct catgaagggg gacatcactc
    12361 cgttcgcgct cacccaggcc cgcagcctgg tgacggcggc agtcctcctg gtctatctcc
    12421 tggccaccaa cccctcaacc ctgcgactca cccgggccac ggcaccctgg gccgtcgcct
    12481 tcggcctctc gctcgcagcc acgcaggtct gctacttctc cgccatagag agattgaacg
    12541 tcgcggcggc catcctcctg gagtaccttg ccccggcgat cgtggtggcc tacagctggc
    12601 tcttcctgcg cagggcaatg acgaagacga cgctcggcag cctcatctcc gtcttggtcg
    12661 gatcggccct ggtggtgaag gcatacgacc ctgcggccct cagcctctcg gcgagcggag
    12721 tggcgttcgg gctgggcgca gccatcgcct tctccgccta catccttata ggcgagcact
    12781 tgcaggcgct ggggaccgga gtggccaccc agctcttcta cggattcacg gtcaccacag
    12841 tcctgctgat ggcgatacgg cctccgtggc gcgcggcccc cgagacattc gaaccgtcga
    12901 acctgatgct gctcgccgtg gtcggcatcc tcggaacgct cctgcccttc gcctgcttct
    12961 tcgcctcgct ccgctacatc gatgccggcc gggccaccat agtgtcgact ctcgagcctg
    13021 tggtagccgg aatggtcgcg ttcgcgtggc tgggagagcg tttcgcgggc atgcagctcg
    13081 tgggagccgc tctgataatc ggagcgatcg tcgctctgca gcgcacggac agcgggaggg
    13141 gcgagatcgt catgcccctg gaagtcgtgg gtttctcgga ggagacggtc gacgaccttt
    13201 ctcgcgcctg accgacccct tcacggtgct ctgccgctat actgtccagc gtgcaccgag
    13261 cgtcaggggg atcgacttgg agcaaccggc gggcactccg cggaccacac tacgcgacat
    13321 caggggtcca ctcctcatgg gagggttcct ggcggtcatc caagcggggg tggtggccgg
    13381 tgtcctctat gctgcgcgac cttcgctcac gtgggccgag atcgcccttc ttccgctcgt
    13441 ggtcgtgtcc accgtgggca tcgtggtctg ggtcgggatg ggacgggaca cgttcaagga
    13501 catctcccgc cagcaggcca tccaagccga gggcatcctc gacatcgtca tcgaatcgct
    13561 ccctcacctg cgccagggcc ttaatcctga atcggctcgc gaggtctcgt acctcatctt
    13621 cgcaaaactg gagttcgatg cggtgtcgct caccgacact acgcgtatcc tatcgttcgt
    13681 cggcgagggg gagaaccacc acctgcccgg aacgaagatc aagaccaagg ccacccagga
    13741 ggctctagac actcgccagg tgcaggtggt caacaaccgc tggaacatcg gctgcccata
    13801 tctagactgc tccctgcact cggccatcat cgcgccgctc atcgtgagag accaggccgt
    13861 gggggcactc aagctctact attcacgccc caaggacatc ggggaactgg aggaggctgt
    13921 ggccatgggc ctcgcgcgcc tcctctccag ccagttggag ctggccgagt tggacaccca
    13981 acgtcaggct acgctgtcgg ctgagttgcg ggcgctccag gcccagatca accctcactt
    14041 cctcttcaac tccctcaaca ccatcgccat gttctgccgc accaagcccg ctgaggcacg
    14101 cagcctggtg ctggccttcg cggacttctt ccgccatacg ctgaggagcc cccaggactt
    14161 catctccatc agcgaggagt tggagtacgt gaacaactac ctgcacctcg agaaggcgcg
    14221 ttttggcccc gaactcgtcg tgaacctctc gctctccccg gaggtcctcg acctcttcat
    14281 cccgccgctc agcatccagc cgttggtgga gaacgcggtg aagcacggaa agggcggggt
    14341 cggcaggcgt ctgacgatcg agttggcggc gtggagcgag ggcgatcacc tacacgtaga
    14401 ggtgagggac gacggccctg gcttcgacac cgacgtctat gcggagatga ggaatcacgg
    14461 cccgtcccag gcccacggca tcggcatctt caacgtgcat cgccgcctgc gcgccttcta
    14521 cggaccccag ttcgcgttca cgctatcgag cagcaaaggc acagggagcg cggtgggttt
    14581 cacgctgccc atggccctct gcctccccga cgatggtccc ggcgccgatc ccacagaccg
    14641 cggcctgata ggagctgtca acggcccgga tgacggctgg gtcgacgacc gcagttcgag
    14701 aggagcattg accgggccgt gagcgatcgt ctaccgcgca acattcctaa cgggaccggc
    14761 agagagcgct atgggacgtg ggacctttcg agcgaccggc acccccccga ggagcagcct
    14821 gaggggacct gggagagcaa ggtagcggat catgcgagtg aacagtcaga cgaggtcatt
    14881 cgcaggttga tcgtacgcca acggcggctc agcatcgcgg ccaccgcact cctcctgggg
    14941 gccgtcgtgg gagtggtgac gatcagccat ctcttcccag agacgatggc ggtgcccgta
    15001 tggcggggct tcagtcccgc gttcctcttc atgggtgtgc tgatctaccc gctcacgtgg
    15061 atcgtggcgg tcctctacgt cgtcatctcc aatcgaatgg acggactccg gtgaaccccg
    15121 tcatcgcgga cttcgagacg cctgtgttcg cgctcgtctt cggcgtcgga ctcctggcgc
    15181 tgagcgtcgc cctagcgtgg aacgtccgca agttgcagaa gacggcggcc gactactacc
    15241 tggccggcag ggcggtgggc atcctgcaaa acgcctccgc catctcggga gattacatct
    15301 cagccgcatc cttccttggc atctcgggac tcacgttcct cttcgggttc gacggacttt
    15361 actacgccgt gggcttcttc cttggattcg tggtcgttct cttcttcatc gcgggtccgc
    15421 tgcgcaagtt cggccagtac accatccccg acttcgtggg aggtcggttc cacacgaacg
    15481 aggggcgggc ggtggcggtg ctgtgcgtcc tggtgatcag tctcttctac acggcgccgc
    15541 agatgctggg cgccgccaag atactcctca tcctcacgca tctaccctac gcggtggccg
    15601 tggccatgat gtcagggtcg atcaccctct acgtcgtgct cggaggtatg cgtgcggcca
    15661 ccatgatcca gatcttccaa ttctgggtgc tgtggtgcgc gatcttcctc gtggccctgc
    15721 tcgtctggca ggggggtctt ccctacgacc atgtggtaac cgagatccag cgtctcacca
    15781 cgcaggagac cggggcgata cggagccagt acatgagccc cgccggcaac cgcgacctgc
    15841 gcgaatccct gtccctgctg ttcgctctgg tgtgcggcac cgccggcctg cctcacatcc
    15901 ttgtgcgcct ctacaccaac ccggacagag cccgcgcgcg ctggagcgtc ctgttggtcc
    15961 tgctcttcat cggcactttc tacctcatga cgccctacat cggcatcgcc atccactatc
    16021 tgttcctgat caccgcatcc ggggcgccgg cgggaaccga gggcctctcg ccccagatca
    16081 tgcactggct caccttcgac gggcagaacc tcgccgtgcc ggccgcggcc atgcgctttg
    16141 ggggcgaact ggcgctcggc atcgccgtcg ccggttcgct cggcgccgtg ctatccacca
    16201 ccgccggtct cctggtggtg atgagcggag cattgggtca tgacctctac tacagcctct
    16261 acaacccgaa ggccagccaa cgcgcgcgcg ttctcgtggc acgcggctcg acggtggtca
    16321 tgggaatcgt cgtgttcctg ctcggactgg ccgtggaacg catgcagatc gccgtgctgg
    16381 tggggttggc gttcgccatc tcagcgtcaa cgctcttccc cgtgttgatc ttgggattgt
    16441 ggtggagacg gatgaccggc cctggggcga tagccggcat ggtcacgggc ttgggcgtca
    16501 gcctcttcct catcttcggc aagtcgctgc tcccggtgtg gctgcgattc gagaatccgg
    16561 gcggagtaag cgtggcagcg agcttcctgg ccatcgtcgt agcatccaag gttttcggcc
    16621 gcgtccccga cgacgtggat cggttcatgg cgctggtgca cggcacgagc gaagagcagc
    16681 ggatccgacg cgcaagccgc atcaagcgac gcaaggtgtt tgccggtctt cctcggcgct
    16741 agcacctcgt cctgatctgc cacccccgcc ttcggcccgc gccgactgcc gggggatacg
    16801 cctcagcgct ggcagtaccg gcagaaggcc gccccccgac ccccaaccac agtccgcacg
    16861 agagggctac cacacgacgc gcacggaagt ccgtgccgtc cgtaggcgcg cagcgcgtcc
    16921 tggaacgagc ccggcgcgcc ctcggtgtcg acgaagtccg agatcgagca gccgcgtagc
    16981 cgcacccctt ccccgaggcg ctcgcggatc tcgcgcgcca gacgctcggt ctcgggccgc
    17041 gtgagagtgc ctgcgggccg cagaggcgac aggcgagcgc cgtacaggat ctcgtcggcg
    17101 tagatgttgc cgatgccggc caggtggcgc tgatccagca agaacgcctt caacggagcc
    17161 gtgcgtccat tcaaacgcga ccggaggaag tcggcgtccc agtctccgat ccacgcgtcc
    17221 ggaccaagac ctgatagtcg agcggcgggt gcgcccaggg tgacatgcag gcgaccgaac
    17281 ttccgtatgt cgcggaagac gaggcgatcc tctccgccgt gaagcaaccc ggagaatcgc
    17341 acgtgcgaga tctcggcccc tctcggcgcg atgaggagtt gccccgtcat accgaggtgt
    17401 atggtcaagt acacgtcgcc ctgcagcggc agcaccaaga acttgcctgc gcgggtgacg
    17461 ctgccgaatg cgcggccctc gatgaggccg ggcaggtcct gggcgtctgc atccctcagt
    17521 atgaaggggt ccaccgattc caccgagacg atctcgcgcc ccggcagcgc ggcgtccaac
    17581 tggcagcgca ccgtctctac ctcggggagt tcgggcacct cggacctaca tgctctcggg
    17641 agcgctgacg cccagcaggt agagacagcg ggcgatgacc cactgcgaca ccaggcacag
    17701 cgccaaccgc gacgccgcca cgtcgtcgcc cgcgccgatg actcggcagt tcttgtagaa
    17761 cacgtggaac cgccccgcga cctcctgggc gtatccggcg atgcggtgtg gcgcacgacg
    17821 gtgcgcggcc tcccgcacga cgtcctcgaa cgacgcgagc accttgacca agtcgcgctc
    17881 gaaggtggtg gagaacacgg ccgcgccttc cgagacaccc gcgagcaact ccgcgccctc
    17941 gccgaggcgc cccgctaggt cctccggaag gttccgaagg atgcttgcga cacgcgcgtg
    18001 ggcgtactgg acgtagaaca cagggttctc ctgcgtctgc tgtcgcgcca acccgatgtc
    18061 caggtcgagt gtctggtcgt gagaacgcat caccaggaag aagcgcgctg catccacgcc
    18121 gatggcctct atcagttcgc ccagagtcac catctcaccc ttgcgggtgg acatctgacg
    18181 aagttcaccc tcctccaaga gattcaccat ctggccgatg atgatctcga gcatgtcggg
    18241 atcgtgcgag agcacctcga cggccgcctt catgcgcggg atgtacccgt gatggtcggc
    18301 tccccagatg ttgatgaggt ggtcgaagcc gcggaggatc ttgtcctggt gatacgcgat
    18361 gtcggcggcg aagtaggtgg gacgttcgtt gctgcggatg agcacgcggt ccttgtcgtc
    18421 gccgtactgg gtggtgcgca gccagatcgc atcgtctttg cgataggcgt ccccggtctc
    18481 gagcagcctg tcgatggtag cctgcaccct cccctcggtg tggagtgggg tctcgctgaa
    18541 ccagcggccg aaggtcaccc cgaacatctg tagctccgtg ctcatctgct tgagcaccaa
    18601 ctcgtagccg cgcacgcgga agtagcttac ggatggcgcg tcggcgtcgt cgatgccgtc
    18661 gcgggccagc gcctcgaccc aacggtcacc gacctccgcg gttatcacgg ccgcgatgcc
    18721 cttcacatac tcgccctggt agccgtcctc cgggacggcg aggtccacgt cgaaggcctg
    18781 ggcgtagcgc gcagccaccg agcggccgaa catctccatc tgccgcccgt agtcgttgat
    18841 gtagaactcg gtggtgaccc ggtgaccact gtgagccaag atgcgggcga gcgcgttccc
    18901 ataggcggcg tagcggccgt gaccgacgtg cagggggccg ttggggttga cgctcacgta
    18961 ctcgagcagg atatgaagcg gcttgtcccg ggtgccgcgg cctacgtcgc tgccggcaac
    19021 cagcatggcc cggacggccc cagcgaacgc cgccgagctc aacgtgaggt tcacgaatcc
    19081 cggcccggcc acgtcgaccc gctccaccaa ggcggagacc tcggggtcgg cgaggaagcg
    19141 tactctcagc tcctcagcga gagccctcgg cggtctgcgc gcgatcttcg ccaactgcag
    19201 acagtacggg acggcgtagt cgccgtggcc ttcgtccgaa ggcctctcca ccacgaggtc
    19261 gggggtaccg gtccatccgt tctctgcgac ccactcctcg accaccctac acacggcact
    19321 ttcaagcaac gacgtactcc tcgtctcagt gcccgccgcc cgatgcgggg atgcggcagg
    19381 cgaacaactc ttccagattg gcgatatcca ccggcgtccc gatggcgatg atgacatcgc
    19441 catcgcctag gatcaggccg gggttcggat tggtgtcgaa cagcccggaa ctcccgtggc
    19501 gcacggccag gatggtcgcc cctgtgcgct tccgcacctc cagctcggcg atcgaccgac
    19561 cgactatctc gcacgtgtcg ttcagcgcga actcctccac gcggaactcg atctcccccc
    19621 caccggccac gacctccaag tagtcgttca ccaacggctt gagcagcagg ccggccatct
    19681 tctgcccgcc gatgctgtag ggggaaatcg cgtgatcggc acctgccttc ttcaccttct
    19741 tcaggg
//
LOCUS       DASRMJ010000087         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_295808, whole genome shotgun sequence.
ACCESSION   DASRMJ010000087 DASRMJ010000000
VERSION     DASRMJ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295808"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VFD74_02610"
     CDS             complement(<1..1603)
                     /locus_tag="VFD74_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZK48478.1"
                     /translation="MAKLPMTARLARLSARRPWLIISVWAAFLILAALAASTIGGVLG
                     AEEMGFTNAPESVRGARLLEDRLRGVTPQTETIVIKSDAATVDDPAFRAVVERVTTDV
                     SALDGAVASAFSYYQVKAVGAPQAEAMVSKDRHATIIQVALVDEAASGGSDRVERLQD
                     ALAGSASPGYQILTIGAASVTKDFTKTAESDLIKAEAFGLPVTLIVLVVVFGAVAAAG
                     VSLLLALTAIVVAIGLTAVVGRVFELSFFVVNMVTMIGLAVGIDYALFIIERYREERR
                     AGAGKHEAITIAGGTASNAVLFSGATVVLALLGLFIVPITTFRSLGIGAVLVVTVAVA
                     AMLTLVPALLSLLGDRVDWPRIPSRLLAVREMRWGGWLRARAHRRRGRPEDHATEHSR
                     WAFSGFWGGIAHIVMRRPIISVAVTVALLVAAAMPYADLRSGSAGAETLPPGDTKTAY
                     TLLETEFPGGLSEPVEIVVAAERVTAVEESISALVKSLDSDPDFVPGARVEWNRAGDL
                     ALVSVQLAAEPNAPEALDAIARLRTQ"
     gene            complement(1906..>2192)
                     /locus_tag="VFD74_02615"
     CDS             complement(1906..>2192)
                     /locus_tag="VFD74_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48479.1"
                     /translation="YGASGWLKAIVDRTYGLLDENSKSRVVAGKKLYVITAQQEADRE
                     DSAIVVRQLERAFSWLGMVPSGSLIGARLDEAGEYPERPELLEAARALIV"
BASE COUNT          388 a          737 c          739 g          328 t
ORIGIN      
        1 cctgtgtgcg aagccgggcg atggcatcga gtgcctcggg ggcgttcggc tcggccgcca
       61 attgcacaga caccagcgcc aggtcgcccg cccggttcca ctcgacccgt gcccccggca
      121 cgaagtcggg gtcgctgtcc agagacttca cgagcgcgct gatgctctcc tcgacggcgg
      181 tgacgcgctc ggcggcgact acgatctcca ccggctccga caacccgccc gggaactcgg
      241 tctcgagcag cgtgtatgcg gtcttggtat cgccgggagg cagcgtctcc gcgccggcgg
      301 agccgctgcg cagatccgcg tagggcatcg ccgccgcgac gagcagggcc accgtcaccg
      361 cgacgctgat gatgggccga cgcatcacga tgtgggcgat gccgccccag aacccgctga
      421 aggcccagcg gctgtgctcg gtcgcgtgat cttcggggcg cccgcggcgg cgatgcgccc
      481 gtgcgcgcaa ccatccaccc catcgcatct cgcgcacggc gagcaggcgg ctggggatcc
      541 gaggccagtc gacccgatcc ccgagcaaag agaggagggc cggcaccagg gtgagcatgg
      601 ccgcgaccgc caccgtcacc acgaggaccg ccccgatgcc gagtgaccgg aaggtggtga
      661 tggggacgat gaacagaccc aacagtgcga ggaccacggt ggcgcccgag aagaggacgg
      721 cgttgctggc cgtgccgccg gcgatggtga tggcttcgtg tttgccggcg cccgcgcgac
      781 gctcttcgcg gtagcgctcg atgatgaaga gggcgtagtc gatgccgacc gccagcccaa
      841 tcatcgtcac catgttgacc acgaagaagg agagttcgaa gacccgtccc acgacggcgg
      901 tgagtccgat ggccaccacg atcgcggtga gcgccagcag caaggacaca ccggcggccg
      961 ctactgcgcc gaacaccacc accaggacga tgagcgtgac cggcaggccg aaggcctcag
     1021 ccttgatgag atcggactcg gccgtcttgg tgaagtcctt ggtcacgctg gccgcgccta
     1081 tggtgagaat ctgatagccg ggcgaggcgc tgcccgcaag cgcgtcttgg agtcgttcta
     1141 ctcggtcgga tccgccggag gcagcctcat ccaccagggc cacttggatg atggtggcgt
     1201 gacggtcttt cgagaccatg gcttcggcct gcggcgcccc gactgccttg acttggtagt
     1261 aggagaaagc tgaggcgacc gcgccgtcga gcgcgctcac atccgtcgtg acccgctcga
     1321 ccaccgcgcg gaacgcgggg tcgtccacgg tggcggcgtc cgacttgatc acgatggtct
     1381 ctgtttgagg tgtgacgccg cgcaggcgat cttcgagaag tcgcgctccc ctcactgatt
     1441 cgggggcgtt ggtgaaaccc atctcctccg cgcccaacac acccccgatc gtggaggcgg
     1501 cgagggccgc aaggatcaag aacgccgccc acactgagat gatcagccat ggacggcggg
     1561 cgctcagccg ggcgagccga gcggtcatcg ggagcttggc cataccttcc ttcttcaaca
     1621 tcggcctcgg tggcgctgga tacgaacaac aagagataga agccaacgat agacctgcta
     1681 cagcgctacc agctgatgtt gccctccccc cgcatatcgg cacgaaccga cgcgggctga
     1741 agtcgtctgg ccgagacgac tgtggagtgg aaggaaggat agcccaggcc gcgagactgc
     1801 gtcaaccgaa gccggggccg gttcgatgaa cggaagggcg acgctcggcg gggccggcgg
     1861 aggggacctc caccgctcgc cccaccgctc gccccagcgc ccgcttcaga cgatgagtgc
     1921 gcgggccgcc tcgagtagtt cgggccgctc cgggtactcg cccgcttcat ccagacgcgc
     1981 gccaataagg gatcccgacg ggaccatccc cagccaactg aaggcacgct ccaactgacg
     2041 caccacgatg gcgctgtctt cgcgatcggc ctcctgctgg gcggtgatca catacaactt
     2101 cttcccggcc acgacccgtg acttggagtt ctcgtcgagg agtccgtagg tgcggtcgac
     2161 gatggccttg agccagccgg aggcgccgta gt
//
LOCUS       DASRMJ010000088        12577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_224443, whole genome shotgun sequence.
ACCESSION   DASRMJ010000088 DASRMJ010000000
VERSION     DASRMJ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12577
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224443"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1091)
                     /gene="pheA"
                     /locus_tag="VFD74_02620"
     CDS             complement(135..1091)
                     /gene="pheA"
                     /locus_tag="VFD74_02620"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZK48480.1"
                     /translation="MSGCAPRGRDLAQWDTSEHPLPLVFVARSLGFLGPTGTFTEEAL
                     RSVLACAPEAEPHERRVIPFPSLLDTVQAVSEGVVDCAVVPIENSIEGSVSATVDILA
                     HGVENLQVVREVRLPIRHRLIAREGVSLEDITHVVSHPHANAQCRGWLRLHLPDASVE
                     AANSTADAVERVAASAEPWAAIGTALAAREYGCVVLEESIEDHDDNETRFVFLSRTRE
                     RQDLPEPYKTSVVCRILKDQPGALLLILQEFAHRYINLTKIESRPSKRSLGDYIFLID
                     LEGKVGDPQVTDALRCLTCKLLEVKVLGSYPMGVPGATSDAF"
     gene            complement(1123..1944)
                     /locus_tag="VFD74_02625"
     CDS             complement(1123..1944)
                     /locus_tag="VFD74_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HZK48481.1"
                     /translation="MNLTELTPFLAIAASAIALLALLLLFVVNRRLKTLLRAQRVVLG
                     SKGEVDVVRYIGSLEEKLVNLRTMVEDLAIAGNDHEVRIDNCISRTGAVRFDAFHDLG
                     GRQSTSVALLNAAEDGVVITSAVSREFARIYVKLVHDGKPDIPLGPEEIEAVDQARAR
                     GNAPFTVRPRPERPRDEDDKLEVDAVDPNAPASESAAAHRALERENRRRKRAGLPPVD
                     ELPPMPSTLGWPKLEPVDDTFASAPDGPASAEEGAEGAPVAPGRAAAASAGAESR"
     gene            complement(1992..3068)
                     /locus_tag="VFD74_02630"
     CDS             complement(1992..3068)
                     /locus_tag="VFD74_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HZK48482.1"
                     /translation="MPTNEDNESFFELADEERRRTGRRGSSAPLVRMLLVLLAVIVIV
                     LVGGFLIRNWLHDRQVTTYETYTRQVSDILKRSDATGREMSDLLLNPADSTRKDVQTK
                     LDQYIATAARLTEEAKALVSPSDLKEAQQWFVATMQLRSRGLENLKPALMNALEVQDI
                     EVASDTISRAMLLLVLSDVTYEEFFVTRATQVLQERQIAGVTVGTTDFVTDSSLATKT
                     KIKEVLTTLKSSDTLQSVHGVALTAVQAMPSEKTIEAGGTYNLTSTDELRFVVTVENQ
                     GNMAETDVPVELTLSSPSSAQPQIVTIKITELKAKEIKKVTVTGVNPSEYGEKALLTV
                     KIGPVPQEKIEENNTLEAHLIFTL"
     gene            complement(3096..3179)
                     /locus_tag="VFD74_02635"
     tRNA            complement(3096..3179)
                     /locus_tag="VFD74_02635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3143..3145),aa:Leu,seq:cag)
     gene            3476..5140
                     /locus_tag="VFD74_02640"
     CDS             3476..5140
                     /locus_tag="VFD74_02640"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZK48483.1"
                     /translation="MIRKLGSGAFATVYLAEDTRMGRSIAIKVVEDADDSDGRAVREA
                     QAAAKLDHAHIVTVHEVVSEPDRTLLFTEYVQGHTLRELYSRRRLTDAQLLRAGIQIA
                     GALEHAHKRGVIHRDIKPENLMLVASEDVDVRVMDFGVAKLEDLSSVTIDGDLVGTLA
                     YMAPEQLAGRPVDPKVDVYSLSLTLYEGFTGSNPLRGKNPAELLRGPSRMVFSRLARS
                     RPDLPAVLDEALQLGLEKDPAKRPDAVALRRMLEQAAKEMPEIESTPTLGERAAAAFS
                     AAARAERLAYAGRHLVAGAASLATAGYVLPRAPFYPEVALLPLAAGAAFLTLLSPAAG
                     GALTLALVAPPFFEFGLGWGILYVLVASSTFALLLWRRKEWLALVPGAAPAMVAIGAG
                     LALPALSGFLARRWGPLVGGLAGLAVAIAGALGVWGTLPFVFSSVSGAGLSSARHVGS
                     PGSAVQALARMFDQRPELLLQVGLFALFAVPWWAMLAGTVPRRLWTATLYLGLMFSAF
                     VLAPPLVAGVSVNVGAFLRAFWPCVIIGYLLALLAPARSSVTAPEG"
     gene            5152..5949
                     /locus_tag="VFD74_02645"
     CDS             5152..5949
                     /locus_tag="VFD74_02645"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HZK48484.1"
                     /translation="MRKTESRVQRAFEGVFGRTFKGYVQPVELAQKLVKEMEEGKLES
                     SSRVYVPHRFIVYLCRRDRERLRTHEASLTKELESHLLQHARREKYDLIGPPEVVIAS
                     DDDLKLGFFGIRPEPVRPRTLPQEARVARPAAAPHAVPFPVVRSPAPSTSRPGLEDTQ
                     GISAGQAMELRLARRTLVLGDALARREFQQSRIVVGRAREADYQVDDPNVSRKHAVLY
                     WEGDQVFVKDLGSTNGTYVNGRPVTAGGLVDGDVLSVGGAKIPVRVL"
     gene            5992..6555
                     /locus_tag="VFD74_02650"
     CDS             5992..6555
                     /locus_tag="VFD74_02650"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZK48485.1"
                     /translation="MSLALLLGKFLFLAVLYLFVLMVVRAGDRDLKAPAGALGSPGRL
                     GRADRRAALPVTDRPPFSASAEGLPVSPSPLAGSAPGRAAPGAKAWALVVDAGPALPA
                     GSAFTIYSGERVVIGRAPDSDIVLEDTFVSSRHASVTAGDDGLVVEDLGSTNGTLVGG
                     LEVEGRLFVGPGATVAIGDSVFVVEER"
     gene            6552..7748
                     /locus_tag="VFD74_02655"
     CDS             6552..7748
                     /locus_tag="VFD74_02655"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZK48486.1"
                     /translation="MIRLTHGAATHLGLVRRQNEDSFVGEPGLYAVCDGMGGARAGEV
                     ASELACRVLADLPKNVGEPELREAIAKANRMIRDRSLADSELSGMGTTLTAAVRSGSA
                     LTIAQVGDSRAYLFHDGELRQVTCDHSLVAEMIRSGRLTPEEAAVHPHRSIITRALGT
                     EELVNPDFFVVSPVAGDKVLLCSDGLSGMVPDDEVQELLARADDPQDAADLLVAAALR
                     HGGEDNVTVVVIFAEESSGGGSGGGFGEEPDGGLDGGPGSGSAVPSDADASDLAGRAF
                     TTADGFGPLVAETDGASDRSFFGGRRRLVTTIVIAALAAAVAAAAFLTFNSTVFFVGT
                     TQGQVVLFQGMPYRVMGVELYGMEESVPVHFEELDMYLQERVDAHELVTKEEGLRFIR
                     GLETTP"
     gene            7745..9031
                     /locus_tag="VFD74_02660"
     CDS             7745..9031
                     /locus_tag="VFD74_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZK48487.1"
                     /translation="MSTRNRELLFLLPAMVLTSVGFAIVWARQVERLEPASLAVGAVF
                     LGLFGLVHLFRRLVVPHADPYLLPITALLSSLGILMIYRIDPRLARLQTIWLVVGLAF
                     FVFVLAVFRRYRRLADYKYLIGLGGIALLVLTMVAGREVYGARLWIRLGPVGFQPPEF
                     AKLFLVVFFAAYLDDVRELLTVSTRRFLGIALPPLRYLAPLLTLWGLGLLLMIFLKDL
                     GTSLLFFCTLLALMYVATGRLLYVVVGLSLFAGGAVVLYHLFPHVQLRVDIWLDPWQD
                     PLGRGYQVVQSLFAFAGGGLFGRGLGAGYLLSASGNTIIPALETDFIFSAIGEELGLV
                     GAVAIILLYLVFVQGGLRIAVRARDDFTRLLATGLTAAFGLQAFLILGGVTKLIPLTG
                     ITLPFMSYGGSSIVANFGLLALLLLASDGAAREEEI"
     gene            9032..10423
                     /locus_tag="VFD74_02665"
     CDS             9032..10423
                     /locus_tag="VFD74_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZK48488.1"
                     /translation="MQRQVRHLWWLFVIMFAALVGQLTYLQVWAAPGLRTHANNTRAV
                     EQEMRVERGLVVSSDGVLLAANRREGAYYFREYPEAALVSPWLGYSSLRYGRAGVERA
                     YNLELAGESDLLTVRNYLDIIMDRPRRGADLLLTLDSRLQRVAVQALGDRTGAVVALD
                     PKTGAVLALTSSPRYDPGRLDDTWDDLIADESRPLVDRALTGRYPPGSVFKLVVAAAA
                     LEEGAVEPGTMFDDDGPWLAGGFRVRNYADHVYGEHDFAQAMTKSVNTTFAKVAVGLG
                     ADTLARYSTAFGMSEELPWRLGGGTGRFPDPDAMDTAHVAQAGFGQGEVLVTPLEMAL
                     ITAGIANGGRIMAPYVVAEVRDYRQAVLERTEPRVWRTPISAATAATLRDMMVAVVKE
                     GTGTAAALPGVSVAGKTGTAEVGEGSPHAWFVAFAPAEDPQIVVAVVIENGGTGGSVA
                     APVARAVLEAGLR"
     gene            10580..>12577
                     /gene="pknB"
                     /locus_tag="VFD74_02670"
     CDS             10580..>12577
                     /gene="pknB"
                     /locus_tag="VFD74_02670"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HZK48489.1"
                     /translation="MIGRVFDERYEIIRKLGAGGMAEVYLAHDRHLGRDVALKVLQSR
                     FADDSQFVERFRREASSAAGLNHPNIVQIYDRGEAEGTYYIAMEYLDGRSLKEIIQQF
                     GPLRVDHITSIASQILEALRFAHRRDVVHRDIKPQNMIVDDEGRVKVTDFGIARAGSA
                     SSMTETGSILGTAHYLSPEQAQGGHVEASSDLYSLGVVMYEMATGKLPFTADNPVAIA
                     MQHVTDPPVVPSALVPDMPHDLEQIILRALAKRPEHRYLTAASFLEDIHRLQRGEAVA
                     PALGFEPSPGFLDYADGATRLMRSPLTPDSRAHRSPGDRTQVRRSSAQGGDGHDEEDL
                     VFEPPARRTVWPWVMVILFLILLAGGIYVVARTLSGGDVTVAVPSLAGKSTDDATTEL
                     DGLGLKLEIKGEEQTDSFEPGLIARQEPGEGEKLRPGESVSVWLAVGKEKVTVPELVG
                     KTEVQGLIELGQANLQSDIRREPSEDQPEGTIFKQDPGASEKVPIGSAVTIWVSTGPS
                     GLQIVMPPVVGLDQQRAMALLGQELKLTVKIVEEPSDKEAGLVVRQFPEAGQPLVEGS
                     AVTIYVSDGSLVPAVPVPTVIGMLKGPAGLKLGAAGFRVLVVEQPVEGYDPGTVIDQE
                     PPGGTKLKSGKTVTIVVAVTVPTTLPTTTTTAPTTTTTSPPT"
BASE COUNT         1848 a         4118 c         4394 g         2217 t
ORIGIN      
        1 agcgaaaggc tgctgcagat ctcccggctc ccgactgaag cggagcccgg gtccgcgggc
       61 ggcaacccgg ggcatcagcc cgcggggccg gtggaagcca cggagcgcgt ggggcgcgag
      121 cgcccggacc cagcctagaa cgcgtccgag gtcgctccgg gcacgcccat cgggtagctg
      181 cccagcacct tcacttcgag caacttgcag gtgaggcagc gcagagcgtc ggtcacctgg
      241 gggtcgccga ccttgccctc gaggtctatc aggaagatgt agtcgccgag gctgcgcttc
      301 gaaggtctcg actcgatctt cgtgaggttg atgtagcgat gggcgaactc ctgcaggatg
      361 aggagcagtg cgcccggctg atccttgagg atccggcata ccaccgaggt cttgtagggc
      421 tcgggaaggt cctgccgctc gcgcgtgcgc gagaggaaga cgaagcgggt ctcgttgtcg
      481 tcatggtctt cgatgctctc ttcgagcacc acacagccgt actcgcgggc agcgagagcc
      541 gtgccgattg cagcccacgg ctccgccgac gccgccactc gttccacggc gtcggcggtg
      601 gagttggccg cctccacgct cgcgtcgggg aggtggaggc gcagccatcc gcggcactgt
      661 gcgttcgcgt gagggtgcga caccacgtgg gtgatgtcct cgagcgacac gccttcacgc
      721 gcgatcaaac ggtggcgaat gggaaggcgc acctcccgca ccacctgaag gttctcgacc
      781 ccgtgcgcga gaatgtcgac cgtcgccgac accgagcctt cgatcgagtt ctcgatgggg
      841 accaccgcac agtccaccac cccttcggag acggcttgaa ccgtgtccaa gagtgaaggg
      901 aaggggatga cgcggcgctc gtggggctcg gcttcgggcg cgcaggcgag gaccgaccgc
      961 agggcttctt cagtgaaggt gccggtaggg ccgaggaatc cgaggctccg cgccacgaac
     1021 accagaggca gcggatgctc ggaggtgtcc cactgcgcga ggtcgcggcc gcgtggggcg
     1081 cacccgctca cggacgttct gcgcccggcg cgcaggcgcc gctcaccggc tttccgcgcc
     1141 ggcggacgct gccgcagccc gccccggagc gaccggcgcc ccttcggcgc cttcctcggc
     1201 tgaggcgggg ccgtcgggag ccgaggcgaa tgtgtcgtcc acaggttcca gcttgggcca
     1261 gccgagggtc gacggcatgg gcggcagttc gtccaccggt gggaggcctg cccgcttgcg
     1321 ccggcggttc tcgcgctcga gcgctcggtg ggcggccgcg gactcgctcg cgggcgcgtt
     1381 cgggtcgacg gcgtcgactt ccaacttatc gtcttcgtcg cgtggacgct cggggcgcgg
     1441 ccgcacggtg aaaggcgcgt tgccgcgggc gcgggcttgg tccacggctt cgatctcctc
     1501 tgggcccagc gggatatcgg gcttgccgtc gtggaccagc ttcacgtaga tgcgcgcgaa
     1561 ctcacgactc acggccgagg tgatcaccac gccgtcctcg gcagcgttga gaagggcgac
     1621 cgatgtgctc tggcgcccgc ccaggtcgtg gaaggcgtcg aagcgcaccg cccccgtgcg
     1681 agagatgcag ttgtcgatgc ggacctcgtg gtcgttgccg gcgatggcca ggtcttcgac
     1741 catggtgcgc aggttcacga gcttctcctc gagcgaccct atgtagcgga ccacgtcgac
     1801 ctcgcccttc gaccccagca ccacccgctg agccctgaga agcgtcttga ggcgacggtt
     1861 gacgacgaag agcaacagca gcgcgagcag ggcgatcgca gacgcggcga tggcgagaaa
     1921 aggtgtgagt tcggtaaggt tcacaggcgg atttcggtca ggcgacaggc tgggcggagt
     1981 aggtgcggcg gctacagcgt gaagatgagg tgggcttcaa gagtgttgtt ctcctcgatc
     2041 ttctcttgag ggacaggccc tatcttgacc gtgagcagcg ctttctcgcc gtattcggat
     2101 gggttgaccc ccgtcacggt aaccttcttg atctccttgg ccttcagctc ggtgatcttg
     2161 atggtcacga tctgcggttg cgccgagctg ggggaggaga gcgtcagctc gacgggtacg
     2221 tccgtttcgg ccatgttgcc ctggttctcg acggtgacca cgaaacggag ttcgtcggtg
     2281 gaggtgaggt tatacgtgcc gccggcctcg atcgtcttct ccgagggcat cgcctggaca
     2341 gctgtcaggg cgacgccgtg gaccgactgc agcgtgtcgg agctcttgag agtcgtgagc
     2401 acttccttga tcttcgtctt ggttgccaga ctggagtcgg tgacgaagtc ggtggtgccg
     2461 accgtgaccc ccgctatctg ccgctcttgg agcacctggg tcgcgcgggt gacgaagaac
     2521 tcctcatagg tgacgtcgga gagaacgagc agcagcatgg cccgcgagat ggtgtcggaa
     2581 gccacttcga tgtcctgcac ttcgagcgcg ttcatgagtg cgggcttcag gttctccaga
     2641 cctctggagc gcagttgcat cgtggccacg aaccactgtt gcgcttcctt tagatcgctt
     2701 ggggagacca gcgccttcgc ctcttcggtg agcctggccg cggtggcgat gtactggtcg
     2761 agcttcgtct gcacgtcctt gcgcgtggag tccgccggat tcaagagaag gtcgctcatc
     2821 tcccggccgg tggcgtcgga gcgcttgagg atatccgaga cctggcgcgt gtaggtctcg
     2881 taggtggtca cctgacgatc gtgcagccag ttccggatga ggaagccgcc taccaggacg
     2941 atgacgatga ccgccagcaa cacaagcagc atgcgcacaa gaggtgccga agaaccgcgt
     3001 cggcctgtgc ggcgacgctc ttcatcagcc aactcgaaga agctctcgtt gtcctcgttg
     3061 gtgggcatca ctgccgatcg taggagggga ggggatgggc gaggggggac ttgaaccccc
     3121 atgagcttac actcactaga ccctgaacct agcgcgtctg ccaattccgc cactcgcccc
     3181 gtcacgcaca cggcgatgcg gcagccgcgc gcggattccg ggcgagtata acaacaagcg
     3241 tatggggcga caatggcgcc cggacggcct ccggtccgca gtgcgcgcgg ctgcttcgga
     3301 ggccggcggc actgccggcc ggccggtctg ccgcccgcgg ccgcaggcga ggcggcgtca
     3361 cggccgcccc gccgcacgcg ccggtctcgg cccgggcggc ctccggaacg tcggccgggt
     3421 ggcgtatact gagacaccca tggcagaggc gccgcttctt cacgatcgat atcgggtgat
     3481 tcgaaagctg ggcagcggcg cgttcgcgac cgtgtacctg gctgaggaca cccgcatggg
     3541 gcgttccatc gccatcaagg tggtcgagga cgccgacgac tccgacggcc gagccgtccg
     3601 ggaggcccag gcggccgcca agctcgacca cgcgcacata gtcacggtgc acgaggttgt
     3661 gagcgagccc gaccgcactc tcctcttcac cgagtatgtg cagggccaca cccttcgcga
     3721 actctactcg cggcgccgtc tgaccgacgc acagctgctc cgcgcgggga tccagatcgc
     3781 aggcgccctc gagcacgcgc acaaacgtgg tgtgatccat cgggacatca agcccgagaa
     3841 cctcatgctg gttgcgtccg aggatgtgga tgtgcgggtg atggatttcg gcgtcgccaa
     3901 gctcgaggat ctctcctccg tcaccatcga cggcgatctc gtgggcacgt tggcctacat
     3961 ggctccggag cagttggcgg gtcggccggt cgacccgaag gtggacgtct actccttgag
     4021 cctcaccctg tacgaaggct tcacgggcag caaccccctg cgtggcaaga acccagcgga
     4081 actcctcagg gggccgtcgc gcatggtctt ctctcgcctg gcgaggtccc ggccggatct
     4141 gcccgcggtg ctcgacgagg cgctgcagct cggtctcgag aaggaccccg ccaagcgacc
     4201 ggacgccgtt gcgctgaggc ggatgctcga gcaggccgcc aaggagatgc ccgagatcga
     4261 gtcaaccccg accttgggcg agagggcggc ggccgcgttc tccgccgcgg cgcgcgccga
     4321 acggttggcc tacgccggcc ggcacctggt ggcgggcgcg gcttcgttgg cgaccgcggg
     4381 ctacgtgttg ccgcgcgctc cgttctaccc cgaggtcgcc ctgctcccgt tggcggcggg
     4441 agcggccttc ctgacccttc tgtctcccgc ggccggggga gcgctgacct tggcactcgt
     4501 tgctccgccg ttcttcgagt tcggcctggg ctggggcatc ctctacgtct tggtcgcctc
     4561 gagcactttc gcgctgctcc tctggcgccg gaaggagtgg ctggccctcg tgcctggggc
     4621 cgcacctgcc atggtggcca tcggcgccgg gctcgcactt ccggcgctct ccgggttcct
     4681 cgcccgccgt tgggggccgc tggtgggagg tctggcaggt ctcgccgtgg cgatcgcggg
     4741 cgcactcggt gtctggggga cgctgccgtt cgtcttctcg tccgtgtccg gcgcgggcct
     4801 ctcctcggcg cgacacgtgg gctctcccgg gtctgcggtg caggcgttgg cgcgcatgtt
     4861 cgatcagcgg cccgagctcc tcctgcaggt gggtctgttc gccctcttcg ccgttccgtg
     4921 gtgggcgatg ctcgcgggca cggtccctcg aaggctctgg acggccaccc tctacctggg
     4981 gctcatgttc agcgccttcg tcctggcgcc gccgttggtg gcgggggtga gcgtaaacgt
     5041 gggggccttc ctccgagcgt tctggccgtg cgttataatc gggtatctct tggctctcct
     5101 ggctccggcg aggagttcgg taacggcccc ggaggggtga acgtgggtgt catgcgcaag
     5161 acggaatcac gcgtccagcg ggccttcgag ggcgtcttcg gccgcacgtt caagggctac
     5221 gtgcagcccg tcgagttggc ccagaagctg gtcaaggaga tggaggaggg caagctggag
     5281 tcgtcctcgc gcgtctacgt ccctcatcgc ttcatcgtct acttgtgccg tcgagaccgc
     5341 gagcgcctgc gcacgcacga ggcgtcgttg accaaagaac tcgagtccca tctgctgcag
     5401 cacgcacgcc gggagaagta cgatctcatc ggaccgcccg aggtggtcat cgcctccgac
     5461 gacgacctca aactggggtt cttcggcatc cggcccgagc cggtgaggcc gcgcacgctg
     5521 ccacaggaag cgagggtggc gcgcccggcg gcggctccgc atgccgtgcc cttcccggtc
     5581 gtgcgatcac cggctccctc gacgagcagg ccgggcctcg aggacactca gggcatctcc
     5641 gccgggcagg ccatggaact caggctcgcg cggcgcacgc tggtgttggg cgacgccctg
     5701 gctcggcgcg agttccagca gagcaggatc gtggtgggca gggcccgcga ggccgactat
     5761 caggtggacg atccgaacgt ctcgcgcaag cacgccgtgc tctactggga aggcgaccag
     5821 gtgttcgtga aggacctcgg gagcaccaac gggacatacg tgaacgggcg ccccgtgacc
     5881 gcgggcgggc tcgtcgacgg cgatgtgctg tccgtcggcg gcgccaagat ccccgtgcgg
     5941 gtgctctgag cgtgtcatga gcctgtgccg gacagcgggc gcggagtcat gttgagcctc
     6001 gctctcctgc tgggcaagtt cctgtttctg gcggtcctct acctgttcgt gctgatggtc
     6061 gtgcgggccg gcgaccgcga tctcaaggca ccggcgggag ctctcggatc tccgggtcga
     6121 ctcgggcgcg ccgaccgtcg cgccgctctt cccgtgacgg atcggccgcc cttctcagcg
     6181 tcggcggagg gtctcccggt ctcgccgtcg cccttggcgg ggtcggcgcc cggccgggcc
     6241 gctccaggag cgaaggcctg ggctctggtc gtcgatgcgg gtccggcgtt gccggcgggc
     6301 tccgccttca cgatctactc gggcgagcgc gtcgtgatag ggagggcgcc cgattcggac
     6361 atcgtcctgg aagacacctt cgtcagctcg cgccacgcga gcgtgacggc cggtgacgac
     6421 ggtctcgtcg tggaagacct cgggagcacg aacggcacgc tcgtcggcgg gcttgaggtc
     6481 gagggtcggc tgttcgtggg tcccggtgcg acggtggcca taggagattc ggtgttcgtg
     6541 gtggaggagc ggtgatccgc cttacccacg gagccgccac gcatctcggc ctggtgcgac
     6601 gccagaacga ggattcgttc gtgggggagc ccggactcta cgcggtctgc gacggcatgg
     6661 gcggagcccg ggcgggtgag gtggcctccg agttggcctg ccgggtcctg gccgacctgc
     6721 cgaagaacgt gggcgagcca gaactgcgcg aagccatcgc caaggccaac cgcatgatcc
     6781 gagacaggag cctcgccgac tcggagctct ccggcatggg caccacgctc acggccgccg
     6841 tcagaagtgg ctcggcgctg accatcgccc aggtgggaga ttcgcgggcc tacctgttcc
     6901 acgacgggga gttgcgccag gtgacctgtg atcactccct ggtggccgag atgatccgga
     6961 gcggccgact cactccggag gaggcggcgg tgcaccctca ccggagcatc atcacgcgcg
     7021 ccctcggcac cgaggagttg gtgaaccccg atttcttcgt cgtgtcgccc gtcgccggcg
     7081 acaaggtgtt gctctgcagc gacggcttga gcggcatggt ccccgacgac gaggtgcagg
     7141 aactcctggc ccgcgccgac gatccccagg atgcggccga cctcctggtc gcagccgcgc
     7201 tccgtcacgg aggcgaggac aacgtgacgg tggtcgtgat cttcgcggag gagtcctcgg
     7261 gcggcgggtc cggtggcggg ttcggcgagg agcccgacgg cgggctcgac ggcgggcccg
     7321 gcagcgggtc ggcggtaccc tccgatgctg acgcgtccga cctcgccggg agagccttca
     7381 cgacggcgga cggcttcggt cccctggtcg ccgagaccga tggcgcctcc gatcgctcgt
     7441 tcttcggcgg ccgccgtcgt ctcgtgacca cgatcgtgat cgccgctctg gccgccgccg
     7501 tggccgccgc cgccttcctc accttcaact cgaccgtctt cttcgtgggg acgacccagg
     7561 ggcaggtggt tctgttccaa ggcatgccct acagggtcat gggcgtcgag ctgtacggga
     7621 tggaggagtc ggtccccgtt cacttcgagg agcttgacat gtatcttcaa gagagggtcg
     7681 atgcccatga gttggtcacc aaggaagaag ggttgagatt catccgggga ctcgaaacga
     7741 ccccttgagc acccggaatc gggagcttct cttcctactc ccggccatgg tgctcacctc
     7801 ggtgggcttc gcgatcgtgt gggcccgcca ggtggagcga ctggagccag cttctctggc
     7861 cgtcggggcg gtgttcctgg gactcttcgg tctggtgcac ctgttccgtc gccttgtggt
     7921 gccgcacgcg gatccctacc tgttgcccat caccgcgctg ctctcctcgc tgggtatcct
     7981 gatgatctac cgcatcgacc cgcggctcgc gcggctccag accatctggc tcgtggtggg
     8041 gctcgcgttc ttcgtgttcg tgcttgcagt gttccgccgc taccggcggc tcgccgacta
     8101 caagtatctg atcggcctcg gcggcatcgc cctacttgta ctcaccatgg tggctggccg
     8161 tgaagtgtat ggagcgcgtc tctggatcag gctgggtccg gtgggttttc agcctccgga
     8221 gttcgcgaaa ctcttcctcg tggtcttctt cgcggcctat ctggatgacg tgcgcgaact
     8281 gctcaccgtc agcacgcgtc ggttcctggg catcgcgttg ccgccgcttc gctatctcgc
     8341 gccgctgctc accctgtggg gtcttggtct cctcctcatg atcttcctca aggatctggg
     8401 gacgagcctg ctgttcttct gcactctgct cgccctgatg tacgtggcca ccggtcggct
     8461 gctctacgtg gtggtgggtt tgtcgctctt cgccggggga gccgtagtcc tctaccacct
     8521 gttcccgcac gtgcagctgc gggtggacat ctggctcgac ccctggcagg acccgcttgg
     8581 tcggggctat caggtggtgc aatcgctctt cgccttcgcg ggcggcggct tgttcgggcg
     8641 cggcctgggg gcgggctatc tgctctccgc ttccggcaac accatcatcc cggccctcga
     8701 gaccgacttc atcttctcgg ccatcggcga ggaactcggt ctggtgggcg ccgtggccat
     8761 catcctgctg tacttggtgt ttgtgcaggg gggcctgcgc atcgcggtgc gcgcgcgcga
     8821 cgacttcacc cgattgctgg cgaccgggct gacagcggcg ttcgggctgc aggcgttcct
     8881 catcctcggc ggcgtcacga agctgatccc acttacgggc atcactctgc ccttcatgag
     8941 ctacggcggc agctcgatcg tggcgaactt cggcctgctt gccctgctcc tgctggcttc
     9001 cgacggcgcc gcccgcgaag aggagatcta ggtgcagcgc caggtgcggc atctgtggtg
     9061 gttgttcgtg atcatgttcg cggccttggt gggccagctc acctaccttc aggtgtgggc
     9121 ggccccagga ttgcgaacac acgcgaacaa cacgcgggcc gtcgagcagg agatgcgtgt
     9181 ggagcggggc ttggtggttt cgtccgacgg ggtgcttctt gcggccaacc gacgcgaggg
     9241 cgcctactac ttcagggagt atccggaggc ggcgctggtg tctccctggc tcggctactc
     9301 gagtctccgc tacggccggg cgggcgtgga gcgtgcttac aatctggagt tagccgggga
     9361 gtccgacctg ttgaccgtgc gcaactatct cgacatcatt atggaccggc ctcgccgcgg
     9421 agccgatctg ctgttgacgc tcgactccag gttgcagcgc gtcgctgtgc aggcgctggg
     9481 cgatcggacg ggcgcggtcg tggccctcga cccgaagacc ggcgcagtgc tggccctcac
     9541 ctcttccccg cgctacgacc cggggcgcct cgacgacacg tgggacgacc tcatcgcgga
     9601 cgagagccgg ccactggtgg accgagccct gaccggacgg tatccgccgg gttccgtgtt
     9661 caagctggtg gtcgccgcgg ccgccctcga ggagggcgcc gtcgagcccg gcaccatgtt
     9721 cgacgacgat gggccttggc tggcgggggg gttccgcgta cgcaactacg ccgaccacgt
     9781 ctacggcgag catgacttcg cccaggccat gaccaagagc gtgaacacca cgttcgccaa
     9841 ggtggcggtc gggttgggcg ccgacactct ggcgcgctac tcgaccgcct tcggcatgag
     9901 tgaggagttg ccgtggcgtc tgggcggcgg caccgggcgg ttccccgacc cggacgcgat
     9961 ggacacggcc cacgtggctc aggccgggtt cggccaaggg gaggtgctcg tcacccccct
    10021 cgagatggcg ctgatcaccg cgggcatcgc caacggcggc cggatcatgg cgccttatgt
    10081 ggtggccgag gtgcgtgact accgccaggc cgtcctcgaa cggaccgaac ctcgcgtgtg
    10141 gcgcacgccc atctccgcgg ccaccgcggc caccctgcgg gacatgatgg tggcggtggt
    10201 caaggagggc acgggaaccg ccgccgccct tcccggcgtc agcgtcgccg ggaagacggg
    10261 gacggccgaa gtgggagagg gtagtcccca cgcctggttc gttgccttcg cgcccgccga
    10321 ggacccgcag atcgttgtgg cggtggtgat cgagaacggc gggacgggcg gctcggtggc
    10381 ggcgccggtg gcgcgggccg tgctcgaagc ggggcttcgt tgaaggcgcg gcgcggtgac
    10441 ggcctcgccg cccgattccc gtggcgcggc cgcgctctgg taacatcgcg gctgaccgtg
    10501 ggggtgggct ccagcagtgc gacgtcttcc gccgcagcgg gatcgcaatg tcgagcttga
    10561 gtgaaggtag aggcaggacg tgatcggacg cgtcttcgat gagagatacg agataatccg
    10621 gaagctgggc gccggtggga tggccgaggt atacctggcc cacgaccgcc acttggggcg
    10681 cgacgtggcg ctcaaggtgc ttcagtcccg gttcgcagac gactcgcagt tcgtggagcg
    10741 cttccgccgc gaggccagca gcgccgccgg gctgaaccat ccgaacatcg tgcagatcta
    10801 cgatcgcggt gaggccgagg gcacctacta catcgccatg gagtacctcg acggtcgctc
    10861 cctcaaggag atcatccagc agttcgggcc gctgcgtgta gatcacatca cctccatcgc
    10921 cagccagatc ctcgaggcgc tccgtttcgc gcaccggcgg gacgtggtcc atcgcgacat
    10981 caagccgcag aacatgatcg tggacgacga aggacgcgtg aaggtcaccg atttcggcat
    11041 cgcccgggcg ggtagcgcga gcagcatgac cgagaccggc tccatcctcg gaaccgccca
    11101 ctacctctct cccgagcagg cgcaaggcgg ccacgtggag gcctcttccg acctctactc
    11161 gctcggcgtg gtcatgtatg agatggccac cgggaaactg cccttcaccg ccgacaaccc
    11221 ggtggcgata gccatgcagc acgtcaccga tccacccgtg gtgccgagcg cactggtgcc
    11281 tgacatgccg cacgatctgg agcagatcat cctgcgggcg ctcgccaaac ggccggagca
    11341 ccgctacctc accgccgcat ccttcctcga ggacatccat cgactacagc gcggggaagc
    11401 ggtggcgccc gctctcgggt tcgaaccttc tcccggcttc ctcgactacg ccgacggggc
    11461 cacccgcctc atgcgctctc cgttgacgcc ggatagccgg gcgcaccggt cgcccggtga
    11521 ccgcacccag gtacggcgct cgtcggcgca ggggggcgat gggcacgacg aagaagacct
    11581 cgtcttcgag cccccggcgc ggcggaccgt gtggccctgg gttatggtga tcttgttcct
    11641 catcctgctt gcgggcggca tctacgtggt cgcccgcacg ctctccggcg gcgacgtcac
    11701 cgtggcggtc cccagcctgg ccgggaagtc cacggacgat gccaccaccg aactggatgg
    11761 gcttgggctc aagctcgaga tcaagggtga ggagcagacg gacagcttcg aaccggggct
    11821 catcgctcgg caggaacccg gggagggcga gaaactgcgg ccgggcgagt ctgtctccgt
    11881 ctggctggcg gtaggcaagg agaaggtcac cgtccccgag ctcgtgggca agaccgaggt
    11941 gcaagggttg atcgagctcg gtcaagcgaa cctgcagtcg gacatccgcc gcgaaccgtc
    12001 ggaggaccag cccgagggga cgatcttcaa acaagacccg ggtgcgtctg agaaagtgcc
    12061 tatcggatcg gccgtcacga tctgggtgag cacggggccg tcgggcctcc agatcgtcat
    12121 gccccccgtg gtgggtctgg atcagcagcg ggccatggcg cttctcggcc aggaactcaa
    12181 gctcacggtc aagatcgtgg aggagccttc ggacaaggag gcgggcctgg tggtgcgcca
    12241 gttcccggaa gcggggcagc ctctcgtgga gggctccgcg gtcacgatct acgtgagcga
    12301 cggctctctc gtgccggcag tgccggtgcc caccgtgatc ggcatgctca agggcccggc
    12361 gggcctcaag ctcggcgctg ccgggttccg ggtgctcgtg gtcgagcagc ccgtagaagg
    12421 ctatgatccc ggcacggtga tagatcagga acctcccggc ggcaccaagc tgaagtcggg
    12481 aaagacggtc acgatcgtgg tggcggtcac ggtccccacc accctgccca cgaccaccac
    12541 gaccgcgccg accaccacaa ccacatcgcc ccccacg
//
LOCUS       DASRMJ010000089         3255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_367992, whole genome shotgun sequence.
ACCESSION   DASRMJ010000089 DASRMJ010000000
VERSION     DASRMJ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367992"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VFD74_02675"
     CDS             <1..152
                     /locus_tag="VFD74_02675"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZK48490.1"
                     /translation="WSDVPVVLPEIGFMTNRDDDRLLSDSAYQDKIAQALADAALRFL
                     QERAG"
     gene            complement(262..1536)
                     /locus_tag="VFD74_02680"
     CDS             complement(262..1536)
                     /locus_tag="VFD74_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZK48491.1"
                     /translation="MTPNRECPGWPGRVRAVTLLLVASALVAGVAASCAAPQPGPRSD
                     AQPATTSEVQPVPGSDSDPASTDGDAAVPTTTTTLPDPVALTLAGMDTRRKAAQLLVA
                     GFAGTAAPPELLELLREGPVGGAILFERNIRDPEQLAALTAALQAAAAAGGSGVPLLV
                     AVDQEGGSVRRVREGVPAAPGARVAAQTMTIAAVEDLAWRVGGALLRLGVNTNLAPVA
                     DVVEDPASFLYGRSYGSDPETVGSYVAAVIEGQERARLISAVKHFPGHGSAVGDSHLG
                     TVASPDDLDRFMTWHLPPFREAIRVGVPMVMVSHVVATGLGESIPSSVSPGVIGGLLR
                     ATLGFGGVVITDDVEMAGSLGGDTVALDAVVAGADMVIVGHDYAAQRAALESLVTGVE
                     QGRLSTARLDEAVTRVLRLKQAYGLLTLAAAP"
     gene            1682..1924
                     /locus_tag="VFD74_02685"
     CDS             1682..1924
                     /locus_tag="VFD74_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48492.1"
                     /translation="MTRAKGFSLVPKTVPLRQRTNFYQEIVEEFVASREKSVVVEGTE
                     RKPATLIQGLRKAVTATGRDNIKVVQRMGDTYLIKV"
     gene            2009..3058
                     /locus_tag="VFD74_02690"
     CDS             2009..3058
                     /locus_tag="VFD74_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HZK48493.1"
                     /translation="MGTLITTIIISLIIVPLVLGSIFKGALVGRRMLKKGAEPASPGK
                     AAEKDGVFRSLGPWAASWAVGILLFVVLAGVSAYTQVEAGTVGVVKRLGQVQGTVFEP
                     GFHFKFPFLDQVVFYSTKVTSYEASEDPSGSLANYTDFVVDSTTSDGQTVQLTFTVLF
                     RIPVEAAAGVAQEVGNMDAVVENVIKAFARSVSREVPKGFSAEALYTQKGQVDAQDSI
                     QARLEEAFSRHGVIIDQYLIRRITFAPDYVVAVEAKQIAKQNALTEENNIAKQEAIKK
                     QTIINAEGEAEKKKIEAAGEAEAIRLKQEALVQNPLIIQYEFVQRLSPNIEWGILPDS
                     IVPLIDVKGMTGTTP"
BASE COUNT          593 a         1111 c         1072 g          479 t
ORIGIN      
        1 gctggtccga cgtcccggtg gtgctcccag agatcgggtt catgacgaac cgcgacgacg
       61 accgcctgct cagcgactcc gcctaccagg acaagatcgc gcaggctctg gccgatgccg
      121 cgctccgctt cctccaagaa cgggccgggt gagcccgctc acgatcgtcg gagccggcta
      181 ggtagggaat cggagccgtc ggcgggttcg cgaccaccgg tggcgggcgc ccggcgaccg
      241 ctctcggatc cgccgggcgc gtcagggcgc cgccgcgagg gtcagtagtc cgtacgcctg
      301 cttgagccgg aggacgcgcg tcaccgcctc gtccagacgg gcggtcgaga gccttccctg
      361 ctccactccg gtcacgagcg actcgagcgc cgcccgctgc gccgcgtagt cgtgaccgac
      421 gatcaccatg tcggcgccgg cgaccaccgc gtcgagcgcc accgtgtcgc cgccgagcga
      481 accagccatc tccacgtcgt cggtgatcac cactcccccg aaccccaagg tcgcccgcag
      541 aagccctcca attacgccgg gcgacacgga ggaaggtatg ctctcgccga gcccggtcgc
      601 gaccacgtgg gacaccatga ccatgggcac gcccaccctg atggcctcgc ggaagggggg
      661 taggtgccag gtcatgaacc ggtcgagatc gtccggcgac gccaccgtgc ccaggtgcga
      721 gtcgcccacg gcgctgccgt gccctgggaa gtgcttgacg gcggagatca gtctggcccg
      781 ctcctgcccc tcgatcacag ccgccacgta cgaaccaact gtctcggggt cgctgccgta
      841 gctgcgcccg tagaggaagg aggccggatc ctccaccacg tctgccacgg gcgccaggtt
      901 cgtgttcact cccagacgga ggagcgctcc cccgacccgc caagccagat cctccaccgc
      961 ggcgatcgtc atggtctggg ccgcgacgcg tgcgcccggg gcggcgggga ccccctcgcg
     1021 gacccgccgc acgctccctc cttcctggtc caccgctacg agcagcggca ccccggaccc
     1081 tcccgcggcg gccgcggcct gcaacgcggc tgtgagcgcg gcgagctgtt cgggatccct
     1141 gatgttccgc tcgaagagga tcgctccccc caccggtccc tcgcgaagaa gctccagaag
     1201 ctcgggcggc gcagctgtcc cggcaaagcc cgccaccaga agctgcgctg ctttccggcg
     1261 cgtgtccatg ccggccaagg tgagtgcgac ggggtccggg agagtcgtcg tcgtggtggg
     1321 cactgcggcg tcgccgtcgg tggaagccgg gtctgagtcc gacccgggga ccggctggac
     1381 ttccgacgta gtggccggct gggcgtccga ccgggggccc ggctggggag ccgcacacga
     1441 ggccgcgaca ccggcgacga gggccgaagc gaccagcagc agagtgacag cgcggacccg
     1501 gcccggccaa cctgggcatt cccggtttgg cgtcacttcc aagctctccc ctccttgctt
     1561 cctaaacata cgcctcggcg tcaggttctg gttgcttgaa aacacattct tacctataat
     1621 cgaatgagtc gtttgaatcg gaacggcata gtagtcactc atctagtagg agagctagca
     1681 catgactcga gcaaaaggat tctcgttggt accgaaaacg gttccgctcc gccagcggac
     1741 gaacttctac caggagatag tcgaggagtt cgtggcaagc cgtgagaaga gcgtagtcgt
     1801 cgaggggaca gaacgcaaac cggcgacact gatccagggg ctgcgcaagg ccgtgacggc
     1861 gacggggcgc gacaacatca aggtggtcca gcgcatggga gacacgtacc tcatcaaggt
     1921 ctgactccgc atctgacggg cgcgttcggt cgcgtccggg cgctacacga aacgcccata
     1981 cgtccgtcca agcgcccaag gagggtgaat gggaacactc atcacaacga tcatcatctc
     2041 gctgatcatc gtgccgttgg tgttggggtc tatcttcaaa ggcgccctcg tcggccggcg
     2101 gatgctgaag aagggcgccg agccggcgtc acccggcaag gctgcggaga aggacggcgt
     2161 cttccgctcg ctcggcccgt gggcggcgag ttgggcggtg ggcatcttgc tgttcgtggt
     2221 gctggccggc gtcagcgcct acacgcaggt ggaggccggg acggtcggcg tggtcaaacg
     2281 gctggggcaa gtgcagggga ccgtgttcga gcccggcttc cacttcaaat tccccttcct
     2341 cgaccaagta gtgttctact ccaccaaggt caccagctac gaagcctccg aggatccgag
     2401 cggatcactc gccaactaca ccgacttcgt cgtcgatagc accaccagcg acggacagac
     2461 ggtgcagctc accttcacgg tgctcttccg catcccggtg gaggcggccg ccggcgtggc
     2521 gcaagaggtg gggaacatgg acgcggtggt cgagaacgtg atcaaggcct tcgcgcgttc
     2581 cgtctcgcga gaggtgccca agggcttctc ggcagaggcg ctctacaccc agaagggtca
     2641 ggtggacgct caagactcca tccaggcgcg actcgaggag gccttctcgc ggcacggcgt
     2701 gatcatcgat cagtacctca tccgccgcat cacctttgcg cccgactacg tggtggcggt
     2761 ggaagccaag cagatcgcca agcagaacgc cctcaccgaa gagaacaaca tcgccaagca
     2821 ggaggcgatc aagaagcaga ccatcatcaa cgccgagggc gaggccgaga agaagaagat
     2881 cgaagccgcg ggcgaagccg aagccatccg cctgaagcag gaagctctgg tgcagaaccc
     2941 actgatcatc cagtatgagt tcgtccagag actctcaccg aacatcgagt ggggcatcct
     3001 gcccgacagc atcgtgcccc tcatcgacgt taagggaatg accggcacga cgccgtagcg
     3061 gggaccgcgg agagagcccg gtcatcgacg tggccgcccg cccgcgacta ctgcgacacc
     3121 gacgatgcca ccccgccggg ccctcccggc ggggtctccc ctggcccacg cgtcggggga
     3181 gccggaggcc ggccacggcg gccacgaagg cggagaagga gcgaagccac agccgcccct
     3241 gccgcgtcga tgccc
//
LOCUS       DASRMJ010000090         4110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_224509, whole genome shotgun sequence.
ACCESSION   DASRMJ010000090 DASRMJ010000000
VERSION     DASRMJ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224509"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VFD74_02695"
     CDS             <1..1255
                     /locus_tag="VFD74_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZK48494.1"
                     /translation="EREGIGAGTVADLPWWDLEDLDACTRCGRCEEACPAFAAGRPLD
                     PKQIVLKSQRVMRARLGDDPDPTAQPPDLFDVIEDTAEVWSCTTCRACVQACPVAIDP
                     LDKILEMRRYLTNEGRLTGSAAKALESMAMRGNPWLLKQEDRMAWAEGSEADVPVMAL
                     IQDPDLLAEGEEPQEVDYLFFVGCAGSYDPRAQSITRSLVRLLWKAGVSFAVLGEEET
                     CTCEAARRMGEEMLFQVGGTTLKETIEQYRFKKIVTACPHCLNTLRNDYPQLGAHFDV
                     VHHSELLLELLRDGRLPAPVGVGVPTTYHDSCYLGRYNGVYDAPRDVVAAAAGRPPLE
                     MEKRRENGFCCGGGGGHMWMEVKIGEPIEFLRTAQALETQADVVATACPYCKIMFDTG
                     LKAPGLEGRAKVLDLAELLDRAYGG"
     gene            1386..2231
                     /locus_tag="VFD74_02700"
     CDS             1386..2231
                     /locus_tag="VFD74_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK48495.1"
                     /translation="MEELRDIRWVARLLGLSERSVYQMVRDGRLPALRVGGRWRFRPA
                     EIEGWLDSVDTRAAPADLIDGGSTRGESGSGEADIAGGRSPEVSLAHVERREFERIVG
                     GIEDTLERRLAFVALLGTASTARGWLPPVVVGGHAVEFYTAGGYATVDIDLVSAHEPL
                     EKLLPEWGFVPRGRHWLHETLGLVVEAPGAYLEAGQRDRVTRVEIAGAIALVLGVEDL
                     IVDRLSACVHWGSEDDCRWAEVLLDLHADRIDRAYLLERARAMEVEERLKAVLDRPDS
                     PAEPA"
     gene            complement(2595..3257)
                     /locus_tag="VFD74_02705"
     CDS             complement(2595..3257)
                     /locus_tag="VFD74_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZK48496.1"
                     /translation="MARPRSKEEVRREEIVDAALRLILDLGYNHVTLADIAGRVGVSK
                     GLISYYFPRKEDVFLAVLDMIVERLSTDFEGFCQADATPPEKIMMIFNNLFGNEKRAR
                     RYYTVVIDYMAQAIRTPQVQEYTQDIYNSYRTYMERIVEDGVRAGDFRPVDVQRTTSM
                     ILGLMEGLVLQWFFDHKGFDLEAAYEMCVDLMNELLMPEGIRADGRPSFMPKMSAPIA
                     ED"
     gene            3444..>4110
                     /locus_tag="VFD74_02710"
     CDS             3444..>4110
                     /locus_tag="VFD74_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48497.1"
                     /translation="MSDELDRAGDAPPDAVAEHTPEEQVAFEERVRSIAVHAEFARIR
                     ALAELEELDEAEREAEAAGDHVAAGGIREQRDEMKRGHLFSVAVLAFLLVVALIVILA
                     VWATRGTDLTESLTGGDSSASVAEIAPPVPAATSAEVLPPEVFRYVCSDCGSTEVTQV
                     PLDLNKQAKYQYGYEAGGSRIAILSVVDGAEYDALAQSLDAAYVKDQSGGEDVGAPKY
                     LDDA"
BASE COUNT          661 a         1292 c         1454 g          703 t
ORIGIN      
        1 ggagagggag ggcatcggcg cgggcacggt ggccgatctc ccctggtggg acctcgagga
       61 tctcgacgcc tgcacccgct gcgggcggtg cgaagaagcc tgccccgcgt tcgcggcggg
      121 ccggcccctc gatcccaagc agatcgtgct caagtcacaa cgggtcatgc gcgcccgtct
      181 cggcgacgat cccgacccca cggcgcaacc tcccgacctc ttcgacgtca tcgaggacac
      241 cgccgaggtg tggagttgca ccacctgccg cgcctgcgtg caggcctgcc ccgtggccat
      301 agacccgctc gacaagatcc tggagatgcg ccgctacctc accaacgagg ggcgcctcac
      361 cggttcggcg gccaaggccc tcgaatccat ggctatgcgt ggcaacccgt ggctgctcaa
      421 gcaggaagac aggatggcgt gggccgaggg ttcggaggcg gacgttccgg tcatggcgct
      481 catccaggat cccgatctgc tcgcggaggg ggaagagccc caagaggtcg actacctgtt
      541 cttcgtcgga tgtgcgggca gctacgaccc ccgtgcccag agtattaccc gttctctcgt
      601 gcgactgctg tggaaggccg gggtctcctt cgccgttctg ggcgaggagg agacctgcac
      661 ctgcgaagcg gcccgtcgca tgggcgagga gatgctcttt caggtgggcg gcaccacgct
      721 caaggagacc atcgagcagt atcgcttcaa gaagatcgtc acggcctgtc cccactgtct
      781 gaacaccctg cgcaacgact acccgcagct gggcgcgcac ttcgacgtgg tccaccactc
      841 cgagttgttg ctcgaactgc tgcgcgacgg tcgcctcccg gcgcccgtcg gggtgggcgt
      901 gccgacgacc taccacgact cctgctacct cgggcgctac aacggggtct acgacgcacc
      961 tcgcgatgtg gtcgcggcgg cggccggccg gccgccgctc gagatggaga agcggcgcga
     1021 gaacgggttc tgctgcggcg gcggcggcgg ccacatgtgg atggaggtca agatcggcga
     1081 gccgatcgag ttcctgcgca ccgcgcaggc cctcgagacg caggcggatg tggtggcgac
     1141 ggcctgcccc tactgcaaga tcatgttcga cactgggttg aaggcgccgg ggttggaggg
     1201 ccgcgccaag gtgctcgatc tggccgaact cttggaccgc gcctacggag ggtgatcagc
     1261 tcccggtagg gaaactcccg gcctgcctcg tcggggtcgc tcttcgcagt ggcaccgtcc
     1321 cgcgagtcgg gatcattggt tcctaaattt gcctaaattt gcccgttatg ggttagaatc
     1381 taagcatgga agagctccgg gacatccgat gggtagcgcg ccttctcggt ctctctgagc
     1441 ggtcggtcta tcagatggtc cgggacgggc gccttccggc acttcgtgtg gggggtcgct
     1501 ggcggttccg gcccgcggag atcgagggct ggctggacag cgtggacaca cgggctgcgc
     1561 cggccgactt gatagacggt gggagtaccc ggggcgagtc cggcagcggt gaagccgata
     1621 tagcgggcgg ccgcagcccc gaggtatccc tggcccacgt ggagcgacgc gagttcgaac
     1681 gcatcgttgg cgggatcgag gacacccttg agaggcgcct ggcgtttgta gccctcctcg
     1741 gtacggccag cacggcccga ggatggcttc cccccgtggt ggttggaggt cacgccgttg
     1801 agttctatac cgccggaggc tacgcgacgg tcgacatcga ccttgtgtcg gctcacgaac
     1861 cgcttgagaa gctcctcccg gagtggggat tcgtgcctcg gggtcgacat tggctacacg
     1921 agactctcgg cctcgtcgtg gaggcgccgg gggcgtacct cgaagccggg cagcgggatc
     1981 gcgtgacccg ggtggagatc gcgggcgcga tcgccctcgt tcttggtgta gaggatctca
     2041 tcgtcgaccg tctctccgcc tgtgtccact ggggttccga agatgattgc cgctgggccg
     2101 aggtgctcct cgaccttcac gcggaccgaa tcgatcgtgc gtatctgctg gagagggcgc
     2161 gtgcgatgga ggtcgaggag cgtctgaagg ccgttctcga ccggccggat tcgccagctg
     2221 agcccgcatg agtgcgggcg ggaccgtcga ctaccaagtg tggcgcgagg agcgtcgccg
     2281 gttgctcgcg agacagctcg agcttctgac gcggcccgcg aaggatcgac ccggcctgag
     2341 caattcgcgg acccaccgac cgcgctccga ggaccattgg gctgcgatcg cagacggggc
     2401 tcgggccgaa ctcgagcgcc gggagacgaa cgggcgctgg gcctgggtcg agagacgacg
     2461 gctgctgatc agctcccggt agggaagctc ccggcctgcc ccgccgatct gaacggcggc
     2521 agggtgctgc ggaggcgtcg ggagtccggg ccgccgcgcc gatccggccg gcggcggatg
     2581 atcccgagct tcgctcagtc ctccgcgatc ggggccgaca tcttcggcat gaagctcggc
     2641 cgcccgtccg cgcggattcc ctcgggcatg agcagctcgt tcattaggtc cacgcacatc
     2701 tcatacgcgg cctcgaggtc gaaccccttg tggtcgaaga accactgcag caccaggcct
     2761 tccatgagcc cgaggatcat cgacgtcgtc cgctgcacgt ccaccgggcg aaagtcaccg
     2821 gcgcgcacgc cgtcctcgac gatgcgctcc atgtaggtgc ggtacgagtt gtagatgtct
     2881 tgggtgtact cctgcacctg cggcgtgcgg atggcctggg ccatgtagtc gatcaccacg
     2941 gtgtagtagc ggcgggcgcg cttctcgttc ccgaagaggt tgttgaagat catcatgatc
     3001 ttctcgggag gcgtggcgtc ggcctggcag aagccttcga agtctgtgga gagccgctcc
     3061 acgatcatgt cgagcacggc gaggaagacg tcttccttcc gcgggaagta ataggagatg
     3121 aggcccttgc tcactccgac tcgtccggcg atgtcggcca gggtcacgtg gttgtagccc
     3181 agatcgagga tgaggcgcag cgccgcgtcg acgatctcct cgcgtcggac ttcttccttg
     3241 ctccgcggtc ttgccatggt tctcccgccc agcatggggc tactgattgg tcggccagat
     3301 aactaccaga atatcacggg ccgtggggca agggcgacgc ttcacgcccg ggtgcccgag
     3361 ggttcgcccg ccgaaggcgc cgaggccggc ttgacgggcc ccaggggggc ttatatggtc
     3421 cactaggaaa ggaggaggca ctcatgagtg atgaactcga ccgcgcaggc gacgcgccgc
     3481 cggacgcggt tgccgagcac acgcccgagg agcaggtggc gttcgaggaa cgcgtacgct
     3541 ccatagcggt tcatgcggag tttgcgcgca tccgggccct cgcggagttg gaggaactgg
     3601 acgaagcaga gagggaggcg gaggcggccg gcgaccacgt ggccgcgggc ggtatccgcg
     3661 aacagaggga cgagatgaag cgcgggcacc tgttctcggt ggccgtactt gcgtttctac
     3721 tcgtggtggc tctcatcgtg atcctcgcgg tctgggccac gcgcggcacg gacctcaccg
     3781 agagcctgac gggcggtgac tcgtcggcgt cggtggcgga gatcgccccg cccgtgccgg
     3841 ccgcgacctc tgccgaggtg ctgcctccgg aagtcttcag gtacgtctgc tccgactgtg
     3901 gaagcaccga ggtgacgcag gttccgctgg atctgaacaa gcaggcgaag taccagtacg
     3961 gctacgaagc cggcggttcg cgcatcgcca tcctgagcgt cgtggacggg gcggagtacg
     4021 acgcgctcgc gcagtccctc gatgccgctt acgtgaagga ccagagcggt ggcgaggacg
     4081 tgggcgcgcc gaagtatctg gacgatgcag
//
LOCUS       DASRMJ010000091         9997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_82868, whole genome shotgun sequence.
ACCESSION   DASRMJ010000091 DASRMJ010000000
VERSION     DASRMJ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82868"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..289
                     /locus_tag="VFD74_02715"
                     /pseudo
     CDS             1..289
                     /locus_tag="VFD74_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(390..821)
                     /locus_tag="VFD74_02720"
     CDS             complement(390..821)
                     /locus_tag="VFD74_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HZK48498.1"
                     /translation="MRGFYCMLLDPVRREDTRAWLARAAGDLRAAANEVAADDPVLGD
                     VVFHCQQAVEKAAKAFLTWHDVPFRKTHDLGVLGGQCAEIDGSLTEILRRAAPMTEYS
                     WKFRYPGEVFEPPREEVDESLAIARSAVLEIESRLPAEVLD"
     gene            complement(793..1143)
                     /locus_tag="VFD74_02725"
     CDS             complement(793..1143)
                     /locus_tag="VFD74_02725"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZK48499.1"
                     /translation="MTRSTQAVTLDPTTNVALAEAVRRLVDVYSPQLVYLFGSAARGD
                     AGPDSDYDILVVLADDAPAELRRARKGYEALWGLGEPVDIVVWTRREFDRRLHLRASL
                     PATVVREGLLLHAA"
     gene            complement(1198..1500)
                     /locus_tag="VFD74_02730"
     CDS             complement(1198..1500)
                     /locus_tag="VFD74_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZK48500.1"
                     /translation="MTVNGVDIPEGALADFCRRHHVRHSAFFGSILREDFGSESDVDV
                     LVEFEPGTRMGLRFFALERELSELVGRKVDLNTPGFLGRALRDEVTASALVQYEAA"
     gene            complement(1754..1969)
                     /locus_tag="VFD74_02735"
     CDS             complement(1754..1969)
                     /locus_tag="VFD74_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48501.1"
                     /translation="MFALGWLLILAGLVFGIMGGLANLFLAQNFAEFWLINLPVAALG
                     LVFGRYLVERAAPGDIESAGSPDRTRS"
     gene            2169..2669
                     /locus_tag="VFD74_02740"
     CDS             2169..2669
                     /locus_tag="VFD74_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZK48502.1"
                     /translation="MAEGLDLSASPVLAGLTVADVDEIERVATIMHYDAGRTIFSEGE
                     VTDAAGIVLSGRVSLRTTLGSEQIDVAEAGPGDLIGWSGLVPPHRFSATAVAGEAATV
                     ALLKTEDLARLSEEDQYLGRILMRNVASLISERLREAHKNSAALVQQLRSYVPSGKAM
                     SSRAPD"
     gene            complement(2735..3730)
                     /locus_tag="VFD74_02745"
     CDS             complement(2735..3730)
                     /locus_tag="VFD74_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK48503.1"
                     /translation="MPDTPLSDRRKRLAAEILLVGVTAVWGWTFVMVKDAVALLPPLP
                     FLAIRFGVATIVMGLGTWAMRARRSGRRGSAARAAAGVHGFRASVAAGRGLRDSVAAG
                     LLMGTFLGLGYIFQTFGLQRTTASNAGFITGTFVVLVPVLQGLVWRIWPDLRAVLGVG
                     LAAVGLFLLSGGSTELHLLGDGLVLLCAISFSAHILATSRYARRHDAAILTVVQLAVV
                     TALCGVLALGAWALGEPLSLEAVWEPSVLVALGVTALFASAAAFYIQTFAQKHASSTR
                     TAVILTMEPVFAGLFGYALAGERLSLAGWAGAAAILCGMLVSELRMEWPRRRSRE"
     gene            3945..5333
                     /gene="rlmD"
                     /locus_tag="VFD74_02750"
     CDS             3945..5333
                     /gene="rlmD"
                     /locus_tag="VFD74_02750"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZK48504.1"
                     /translation="MTKPHRGETLELSITGLAFGGQGIARLDDFVVFVAGGVPGDLVR
                     ATTLRVKKRYAEARLDEIVEPSADRVPAMCRHYGTCGGCRWQSLAYDVQLRYKQQQVV
                     DSLERLGKLTDFSVQPIKAMDDPWRYRNKVEFSIGRTDGDLVVGFHPPGRWDAVLPQT
                     ECHLLPRATEGVRATVEEWLRQVGATPWDAREGEGYARQLSVREAHGTGEVLVSLVTA
                     PGPLPRPESLVGLLRERHPTVVGVLHAQNGGGADVANGVPYEVLWGRPHLYELLGDLR
                     LKISIDAFFQTNSHMAPVLYATAAEEAALSGGEVVWDLYSGIGSIALFLAGRSRSVLG
                     IEIVPAAVRDAVENAVENGVTNTMFLEGNARVVLKEVLEGRRALPLEVTRPDVVVVDP
                     PRGGLAHKVIRRVAAAEPAKVVYVSCNPTTLAPDAALFGELGYRLTRVTPVDMFPHTP
                     HIEAVALLERGH"
     gene            complement(5392..6105)
                     /locus_tag="VFD74_02755"
     CDS             complement(5392..6105)
                     /locus_tag="VFD74_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48505.1"
                     /translation="MARVALWAVAAVAVLELAPRVPGVGSIEVRVLLGFALGLVPVLL
                     MSWTLVKGVLSPAALGALIVVGIVSAVLLSRLGWFAAAVPFKVVLAASIGVLLGRQVA
                     EAWWLVVVAGVALVADVWSVFAGPTKVVVEKAPVVLDYLLIHFPVLGVAGSGMGLGMS
                     DVIFLSLFAAGSAGAGLRPVAGFLAMAASFLVTVTVALVWKPALPALPFLAVAFLAVN
                     SGRFVGLARSARDRRRGSG"
     gene            complement(6141..6785)
                     /locus_tag="VFD74_02760"
     CDS             complement(6141..6785)
                     /locus_tag="VFD74_02760"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HZK48506.1"
                     /translation="MATEPRRKAERFSVPEDGNEIYHKLEERAIAESNDLNRMVSQCR
                     RCQRGDFLPTVGSGHPLADILMVKYGPRYLEVSEGVAFFGRSGAAILKSVERLAVDPL
                     LLYGTNAVKCPGVESAEGEQNCPGFLLEEIQITQPKMLVVMGEKALEVLNRHRLVTMA
                     EISWRPGEIQVFTPFCRVLVTPDIDDSLDDGDAKRAFWNAFRALGDWYRDEPPY"
     gene            complement(6892..8886)
                     /locus_tag="VFD74_02765"
     CDS             complement(6892..8886)
                     /locus_tag="VFD74_02765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK48507.1"
                     /translation="MSDRSLRVALVTPFSWVRPSSVNQHVADLSRELLRRGHRPVVLT
                     SSDDAGELRRMRRLTRHHDQLALGLLAQWRPGTAPDGRLLPSVGSGPLGPEHGVPVVA
                     LGRSFSFRLNGSVTSIGLPVDVHSRLEHLLVGGGFDVVHVHEPFAPSVSFTAIRESRS
                     PVVATFHLTPAALVAYELGAGVLSRFFNLLDERIVTSMPAADVLAGLFGGSYRVISPG
                     TAITSEEGEPASGTEGPAGARSRIALYVFRGDDTRGLRALMRSLVGAFPAGLDGLRVA
                     VHRPSAGLWPPRPAPRSLRSQVEWVDFDHPSELASRYRDAAVTILPFLGGEWLLQTAA
                     EALAMGCPVVSPDLPVFRGLVEDGGAWSCFDPARDAGLGEAIARATGAETTPVAAHQM
                     ERVASATVEAYADALRRSVGSGGPVERDGGRVPSRVTKMRRRHRGPILSPGGWIHADL
                     HMHTEFSKDSTSSVEALLATAREIGLGAIAVTDHNAIEGALLARELAAGDLTVIVAEE
                     IMTQHGEVIGLFIEELIPKGHTFDETLSMIKEQGGLVYIPHPFDRLRTTPSYRMMVDN
                     LHRIDVVETFNARNMLPQFNVTAERFAAKYNVAAGAGSDAHVLPGLGTAMLRMPRFRG
                     PEEFMASLREADIVTRRKSLLYLQSLKLLRSTLDHVLPGS"
     gene            complement(8947..>9997)
                     /locus_tag="VFD74_02770"
     CDS             complement(8947..>9997)
                     /locus_tag="VFD74_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZK48508.1"
                     /translation="PGLMHVNLGGYHISDIEISAAFDIDILKVGKDVSEAIFSGLNNT
                     VKFSDVPKLNIPVLRGMTHDGLGKYLSEVITKAPGSTVDIAQVLKDTGTDVVVNYLPV
                     GSEMATKWYVEQVLEAGCAFVNCIPVFIAREDYWRKRFEERGLPVIGDDIKSQVGATI
                     VHRVLAHLMRERGVRLERTSQLNVGGNTDFLNMLERERLESKKISKTNAVTSQLDYDM
                     GAKNVHIGPSDYVEWLDDRKWAYIRLEGRTFGDVPLNIELKLEVVDSPNSAGIVIDAV
                     RCAKLALDRGIGGALYGPASYFMKSPPAQYSDSEAHDATEAFIADAPATATSPAAKAA
                     ASPSAAKAEPPAETA"
BASE COUNT         1782 a         3415 c         3312 g         1488 t
ORIGIN      
        1 gctcgcccgg gcgctcgccg agttcatgtt cgacgacgag aaggccatgg tgcgcatcga
       61 catgagtgag ttcatggaga agcactcggt agcgcggctc atcggcgccc ctcccggcta
      121 cgtgggctac gacgagggcg gctacctgac ggaggccgtc cgtcgtcgcc cctactccgt
      181 gctcctccga gacgagggga aactcaccga cggccacggc cgcacggtga gtttcgggaa
      241 cgtggtggtc atcatgacct cgaacatcgg cagccggtgg atcctcgact ccgatcccgc
      301 ctacgaaccg agccggaaca tgggaagaga ccaggtcctg gatctcccct taggccgctt
      361 gtcctcatgg gggccagggg ccgtttcgcc tagtcgagca cctcggccgg gagccgagat
      421 tcgatctcga gaaccgctga cctcgcaatg gccaacgatt cgtcgacttc ttcccggggc
      481 ggctcgaaca cttccccagg gtagcggaac ttccaggagt actcggtcat cggagcggcg
      541 cgcctcagga tctcggtcag ggagccatca atctcagcgc actgcccgcc aagaaccccg
      601 aggtcgtgcg tcttgcggaa cggcacgtcg tgccacgtga gaaaagcctt ggccgccttc
      661 tccaccgcct gctggcagtg gaacacaacg tcgccaagca ctgggtcgtc ggcggccacc
      721 tcgttggcgg cagcacgcag gtcgccagcc gcccgcgcga gccaggcccg agtgtcttcc
      781 cttcttaccg ggtcaagcag catgcagtag aagcccctca cgaaccaccg tcgccggcag
      841 cgacgcccgc aggtgaagac gccgatcgaa ctcgcggcga gtccacacga caatgtcaac
      901 gggctctccc agaccccaaa gggcctcgta gccttttctg gccctccgga gctcggccgg
      961 cgcgtcgtcg gccaagacca cgaggatgtc atagtcgctg tccggtcccg catcgcctcg
     1021 ggccgcggaa ccgaacaagt agacgagttg aggagagtag acgtcgacca gcctgcggac
     1081 ggcctccgcc aaagcgacgt tggtggtggg gtcaagtgtg acggcttggg tgctccgagt
     1141 catccgtgtt ccctccggga tcgacgttcc ccccgtcctg agaagcgccc gcgatcgtca
     1201 ggctgcttca tactggacca gcgccgaagc cgtcacctcg tctcgaagag cgcggccgag
     1261 gaagccaggc gtgttcaaat cgaccttccg cccaaccagt tcggatagtt cccgctcaag
     1321 cgcgaagaag cgcaaaccca tacgggtgcc gggttcaaac tccaccagga catcaacgtc
     1381 gctctcggag ccgaaatctt cccgcaggat ggagccgaag aacgccgagt gccgaacatg
     1441 gtgccgccgg cagaaatccg cgagtgctcc ttcggggatg tcgactccgt tgaccgtcat
     1501 gaccaggcga acctcttctt cagggctcac gaccgggggg cgatttgctg gggggtgccc
     1561 gcttggtgcc tatcgtacca ccgcgacgat caagccggcg ccccgactcc gtactccgtc
     1621 cggacgggcg tcgccatccc aacagccacg gacgtgcagt ggccacggcg cctcaacctc
     1681 cgatcgccgg gtcgcggcgg cccggtcggc cgccgccgac aacccgccga caacccgccg
     1741 accgcccgcc ggtctaggag cgggtcctgt cgggtgaacc ggcgctttcg atgtcgccgg
     1801 gcgccgcgcg ttcaacaagg tagcggccga agaccagacc gagcgccgcc acgggaaggt
     1861 tgatcagcca gaactccgcg aagttctgcg ccaagaagag gttggccaag ccccccatga
     1921 tgccgaagac aagccctgcc aagatgagta gccacccgag tgcgaacatg gatccccctg
     1981 tatgtgatag tgcactcaag cacaagatgg tcggttcccg tccgccccgc cgcggccccc
     2041 gccgaccttc aggctcgccc catcattgcc gctgcgggcg gatgtgtcaa ggtgctccac
     2101 tcctccggat cggacgggcc gaggcgctct gatatgattt gagcaaggcc ggaccgagga
     2161 ggatcatcgt ggcagaagga ctggatctat cagcatcgcc cgtcctagcg ggcctgaccg
     2221 tagccgacgt cgacgagatc gagcgcgtcg cgaccatcat gcactacgac gccggtagga
     2281 ccatcttctc agagggtgaa gtcacagacg ccgccggcat cgtactctcc ggtcgggtga
     2341 gtctgcgcac gactctcggc tctgaacaga tcgacgtcgc cgaggcggga cccggcgatc
     2401 tcatcgggtg gtcgggcttg gtgccgcccc accggttctc cgcgaccgcc gtggccgggg
     2461 aagccgccac cgtcgcgctc ttgaagaccg aggatctcgc acgcttgagc gaagaagacc
     2521 agtatttggg gcgcatcctc atgcgcaacg tggcctcact catctcggaa cgcctgcgcg
     2581 aagcgcacaa gaattcggcg gcgctcgtgc agcagctcag aagctacgtt ccttcgggca
     2641 aggccatgag ttcgagggcc cccgactgac ctcggtcggg gggcgtcgcc tcggttcggg
     2701 tcgggtgacg ccggacacca cggcgtccga ggtgctactc gcgcgaccgt cggcgcggcc
     2761 actccatcct cagctccgac accagcatcc cgcacaggat ggcggccgcc cccgcccacc
     2821 ccgctaggga tagccgctct cccgccaggg cgtagccgaa cagccccgcg aacacgggct
     2881 ccatggtcag gatgacggcg gtgcgggtgg acgaagcgtg cttctgggcg aaggtctgga
     2941 tgtagaacgc ggccgccgaa gcgaacagag ccgtcacgcc gagggccacc agaactgagg
     3001 gctcccacac ggcctccaac gacaagggct cacccagagc ccatgccccg agcgccagaa
     3061 cgccacagag cgcggtcacc accgccaact gcaccaccgt cagtatcgcc gcgtcgtgcc
     3121 gtcgcgcgta gcgcgaggta gcgaggatgt gcgccgagaa gctgatggca cacagcagga
     3181 cgagcccgtc gccgagaagg tggagttcgg tggaccctcc agagagaagg aagagaccga
     3241 cagccgcgag acccactccg aggacggccc ggaggtcggg ccagatgcgc cagaccagac
     3301 cctgcagcac cggtaccagg accacgaagg tccccgtgat gaagccggcg ttggaggccg
     3361 tggtgcgctg gagcccgaag gtctggaaga tgtagccgag acccaggaac gtccccatga
     3421 gaaggccggc cgccacggag tccctcagac cgcgaccggc cgccacggag gccctgaagc
     3481 cgtgaacccc ggccgccgcg cgtgcggccg atccccgccg gcccgatcgg cgggcgcgca
     3541 tcgcccacgt cccgaggccc atcacgatgg tcgcgacccc gaacctgatc gccaggaagg
     3601 gtagcggcgg cagaagtgcg accgcgtcct tgaccatgac gaaggtccag ccccacacag
     3661 ccgtcacccc cacgagaagg atctcggcgg ctaggcgttt gcgccggtcg gatagcgggg
     3721 tgtcgggcaa ggcgctccgt tcgacggggc catgatggtg ggccggatgg ccggtcggta
     3781 tactaccaga ccgcgcaccg cgcaccgctc gtcgaccgct gggagacccg gcacgttgag
     3841 ggcggcccac cggcaccctg cgggagccct gcgggccggc cgccgatagg ctgcccgggc
     3901 gcgcggatcg cccgctccac ccaccgacat ctggagagtc catcttgacc aagccgcacc
     3961 gcggggagac cctcgaactc tcgatcaccg gcctggcgtt cgggggccaa ggcatcgccc
     4021 gcctggacga cttcgtggtc ttcgtggccg ggggcgtccc cggcgacctg gtccgggcca
     4081 ccactctccg cgtcaagaag cgctacgcgg aggcccgcct ggacgagatc gtcgaaccct
     4141 cggcggatcg cgtgccggcc atgtgccgcc actacggcac gtgcggcggc tgccgctggc
     4201 agagcctcgc ctacgacgtc cagctccgct acaagcagca gcaggtggtc gactccctcg
     4261 agcgcctcgg gaaactgact gacttctccg ttcagcccat caaggcgatg gacgatccct
     4321 ggcgataccg caacaaggtg gagttctcca taggccgcac cgacggggac ctcgtggtgg
     4381 gcttccatcc gccgggaagg tgggacgcag tccttcccca gaccgaatgt cacctgctcc
     4441 cgcgggccac cgaaggagtg cgggcgaccg tcgaggagtg gctgcgccag gtcggagcca
     4501 ccccctggga cgcgcgtgaa ggcgaaggct acgcccggca actctcagtg cgcgaggcgc
     4561 acgggacggg agaagtcctg gtgagtctgg tcacggcgcc gggaccactc ccccgaccgg
     4621 agagcttggt cggcctgctc cgcgaacgcc accccaccgt ggtcggcgtc ctgcacgcgc
     4681 agaacggagg cggagcggac gtggccaacg gcgtccccta cgaagtcctc tgggggcgcc
     4741 cgcacctgta cgaacttctg ggggacctgc gcctcaagat ctccatcgac gccttcttcc
     4801 agacgaactc gcacatggcg ccggtgttgt acgccaccgc cgccgaggaa gccgccctct
     4861 ccgggggaga ggtggtgtgg gacctctatt ccggcatcgg gtcgatcgcc ctcttcctgg
     4921 ccggacgatc gcgctccgtc ctggggatag agatcgtgcc tgccgcggtg cgggacgctg
     4981 tggagaacgc tgtggagaat ggtgttacca ataccatgtt cctggaaggc aatgcccgag
     5041 tggtgctcaa ggaggtgctc gaaggccgcc gcgcactgcc gctcgaggtg acccgcccgg
     5101 acgtcgtggt ggtggacccc ccgcgcggcg ggctggccca caaggtcatc agacgcgtgg
     5161 cggcggccga accggcgaag gtggtgtacg tgtcgtgcaa tcccacgacc ctcgctcccg
     5221 acgccgcgct cttcggcgaa ctcggttacc gcctcacccg agtcaccccg gtggacatgt
     5281 tcccgcacac gccacacatc gaggccgtgg cgctgctgga gcgcgggcac tagccgagcg
     5341 tgggcctcag tccggaaccg gctcccgggc ttccagcccg ggaggaccgc gctacccgga
     5401 accgcggcgc ctgtcccgcg ccgagcgcgc gagccccacg aaccgacccg agttgactgc
     5461 cagaaacgcc acggccaaga agggtagcgc cggcagcgcg ggcttccaga cgagcgcgac
     5521 cgtcacggtc accaggaacg atgcggccat ggcgaggaag ccagcgaccg gccgcaggcc
     5581 ggcgccggcg gagccggccg cgaacagtga caggaagatc acgtcgctca tgcccagacc
     5641 catccccgac cccgcgacgc ccagcaccgg aaagtggatg agcaggtagt cgagcaccac
     5701 gggcgccttc tccaccacga ccttggtggg tccggcgaac acgctccaca cgtcggcgac
     5761 gagcgcgacg ccggccacga cgaccagcca ccacgcctcc gcgacctggc gtcccagcag
     5821 cactccgatg ctcgccgcga gcaccacctt gaaggggacg gccgccgcga accagccgag
     5881 tcgggagagg aggaccgcgc tgacgatacc caccacgatg agcgcgccca gcgccgcggg
     5941 ggagagcacg cccttcacta gggtccagga catgagcagg acaggcacca gaccgagcgc
     6001 gaaaccgaga agcacgcgca cctcgatgga gcccacgccg ggcaccctgg gtgcgagctc
     6061 aagaacggcg accgcggcca cagcccagag agctacccgc gccaacacgg gcctcggcct
     6121 acggagcgcg gccgtggtca tcagtagggt ggttcgtccc ggtaccaatc gccgagagcc
     6181 cggaaggcat tccagaaggc ccgtttggca tcgccgtcat caagcgagtc gtcgatgtcg
     6241 ggcgtgacga gcacacggca gaacggggtg aacacctgga tctcaccagg gcgccaagag
     6301 atctcggcca tggtgacgag acggtggcga ttgagcacct ccaaagcctt ctctcccatc
     6361 accacgagca tcttgggttg ggtgatctgg atctcttcga gaaggaatcc ggggcagttc
     6421 tgctcacctt ccgcggattc cacgcccggg cacttgaccg cgttggtgcc gtacagaaga
     6481 aggggatcga ccgccagccg ctccacgctc ttcaggatcg cggcgcccga tcggccgaag
     6541 aaggcgacgc cctcactgac ctcaagataa cgcgggccgt acttgaccat gaggatgtcg
     6601 gcgagggggt gcccggaacc gaccgtgggc aggaagtcgc cgcgctggca acggcggcac
     6661 tgcgacacca tccggttgag atcgttgctc tcggcgatcg cccgctcctc cagcttgtgg
     6721 tagatctcgt tcccgtcttc ggggacagag aagcgctcag ctttccttcg gggttccgta
     6781 gccatggacc ttcctttggg acccgggggg accgtgtccc ggggctgggg aacatattct
     6841 cccccgcgag ggctaagtcc ttcgcagagc gcgcaagatc gagggcccag cctacgaccc
     6901 gggcagcacg tggtcgaggg tcgagcgcag caacttgagc gattgcaggt agagcaggct
     6961 cttgcgccgc gtcactatgt cggcttcgcg cagggatgcc atgaactcct cgggtccacg
     7021 gaaccggggc atgcggagca tggcggtccc cagtccggga agcacgtgcg cgtcgctgcc
     7081 ggcgccggcg gccacgttgt acttggcggc gaagcgttcc gcggtgacgt tgaactgcgg
     7141 cagcatgttg cgcgcattga acgtctcgac gacgtctatg cggtgcaggt tatcgaccat
     7201 catccggtac gagggggtcg tacgcaaccg gtcgaacgga tgggggatgt acacgagtcc
     7261 cccttgctcc ttgatcatgg agagagtctc gtcgaaggtg tgccccttgg ggataagctc
     7321 ttcgatgaag aggccgatca cctcaccatg ctgggtcatg atctcctcgg ccacgataac
     7381 cgttaggtcg ccggcggcca gctcgcgcgc gagcaacgca ccttcgatgg cgttgtgatc
     7441 ggtcaccgcg atcgcgccta gaccgatctc gcgggcggtc gcaaggagag cctccactga
     7501 gctggtgctg tccttggaga actccgtgtg catgtgcaga tcagcatgga tccagcctcc
     7561 aggcgacagg atgggccccc tgtggcgacg ccgcatcttg gtgacccgag aagggacgcg
     7621 cccgccgtcc cgctccaccg gcccgccgct gcccacggac cgtcgcagcg cgtctgcgta
     7681 ggcctccacc gtggcggagg cgacccgctc catctggtgg gcggcgaccg gggtcgtctc
     7741 cgctccggta gcgcgcgcga tggcctcgcc caggccggca tcccgagcgg gatcgaagca
     7801 cgaccacgca ccgccgtctt caaccagacc ccgaaagaca gggagatcgg ggctcaccac
     7861 cggacatccc atggccaagg cttcggctgc cgtctgcagc aaccactcgc cgcccaggaa
     7921 cggcaggata gtcacggccg cgtcacgata gcgggaggcc agctcggaag gatggtcgaa
     7981 gtccacccat tcgacctgag aacggagtga cctcggagcg ggccggggcg gccacaaccc
     8041 ggccgagggg cggtgcacgg ctacccgaag accgtcgagg ccggcaggga aggctcctac
     8101 cagcgaccgc atcaacgcgc gcagccctct ggtgtcgtcc cccctgaaca catagagggc
     8161 gatgcgagac ctggccccgg cggggccttc cgtccccgaa gcgggttcgc cttcttcgct
     8221 ggtgatggcg gttccggggg agatcaccct gtatgaacca ccgaacaggc cggccagcac
     8281 atccgccgcg ggcatcgagg tgacgatgcg ctcgtcgagc aggttgaaga accgcgatag
     8341 gacaccggca ccgagctcgt aggccaccag agccgctgga gtaaggtgga aggtggcgac
     8401 aacggggctg cgtgattcgc ggatagctgt gaagctcact gagggcgcga agggctcgtg
     8461 gacgtgcacg acgtcgaaac ctccgcccac cagcagatgc tccagccgcg agtgcacgtc
     8521 gaccggcagg ccgatgctgg tgaccgaacc gttgagtcgg aacgagaagc tgcgaccgag
     8581 cgccaccacc gggacgccgt gctccggccc cagggggccg gagcccaccg acggcagaag
     8641 gcggccatca ggcgccgtcc ccgggcgcca ctgcgccagc agccccagag ccagttggtc
     8701 gtggtgtcga gtgaggcggc gcatgcgccg caactcaccg gcatcgtcgg acgaggtgag
     8761 caccacgggg cgatgtccgc ggcgcaggag ttcacgggag agatcggcga cgtgttgatt
     8821 caccgaactc ggccgcaccc aggagaacgg agtgaccaga gccaccctca ggctccggtc
     8881 actcacctct ctcgcaccca cacgcgcgtc cgcttccatc ggatacgcgt ctacccgccc
     8941 gctgcgtcag gcagtctcgg ccgggggctc cgctttcgcc gccgagggcg acgcggcggc
     9001 tttcgcagcg ggcgatgtgg ctgttgccgg cgcgtctgcg atgaaggctt ccgtcgcgtc
     9061 gtgggcctcc gagtcgctgt actgcgcggg gggcgacttc atgaagtacg aggcggggcc
     9121 gtacaacgcg ccgccgatcc cacggtccag ggcgagcttg gcgcagcgga ccgcgtcgat
     9181 gacgatgccc gccgagttgg gggaatccac gacctcgagt ttcaactcga tgttcagggg
     9241 cacgtcgccg aaggtgcggc cttcgagacg gatgtacgcc cacttgcggt cgtccaacca
     9301 ctcgacgtag tcggacgggc cgatgtgcac gttcttggcg cccatgtcgt agtcgagttg
     9361 cgaggtgacc gcgttggtct tggagatctt cttgctctcc aggcgctcgc gctccagcat
     9421 gttgaggaag tcggtgttgc cgcccacgtt cagctggctg gtgcgttcca gacgcacgcc
     9481 ccgctcacgc atgagatggg ccaagacgcg gtgcacgatg gtggctccca cctgcgactt
     9541 gatgtcgtcg ccgatcaccg gcaggccgcg ctcctcgaag cgcttgcgcc agtagtcctc
     9601 gcgggcgatg aacaccggga tgcagttcac gaaggcgcag cccgcctcga ggacctgctc
     9661 cacgtaccac ttcgtggcca tctcgctgcc gacgggcagg tagttgacca ccacgtcggt
     9721 gccggtgtcc ttgaggacct gggcgatgtc cacggttgac ccgggcgctt tggtgatcac
     9781 ctcggacagg tacttgccga gcccgtcatg ggtcatgccc cgcagcaccg ggatgtttag
     9841 cttgggcaca tcactgaact tcaccgtgtt gttcaggccg ctgaagatcg cctcggacac
     9901 gtctttgccg accttgagga tatcgatgtc gaacgccgcc gagatctcga tatcggagat
     9961 gtggtagcca ccgaggttca cgtgcatgag cccgggc
//
LOCUS       DASRMJ010000092         5183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_296166, whole genome shotgun sequence.
ACCESSION   DASRMJ010000092 DASRMJ010000000
VERSION     DASRMJ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296166"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VFD74_02775"
     CDS             complement(<1..1419)
                     /locus_tag="VFD74_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZK48509.1"
                     /translation="MYVRCRLLRGTGRTGGRMGQRAYRIRAVLFDFDGTLTLPGALDF
                     AAIREAIGCPAGEPILEFIESRADEGERSRLSAVLDRFEAAAAALAVPAEAAESIVRG
                     LRSDGVRLAIVSRNSQGSIESSLRNFFGIGIEDFDVVASRDIPGRPKPAPDAIHYVLD
                     RLGVDAGEALVVGDHQLDVAAGHAAGCLTALLAPTGAALGGDPAPSFIGLPRIRPDFE
                     IESLVEVSRIVMLGRPLPLGKFPNDLLHEYLEGVIADDPALLIEPAVGEDVAAVDISA
                     EQVLVLKSDPITLVTDSIGEYAVLVNANDIATSGATPRWFIATALFPPGTTPSMVIRA
                     LLDLCDSCRRHGITLCGGHTEITEAVVRPIVVGTMAGTVERARLLDKRNMAPGDRVLL
                     TKGVAVEGTAIIAGELADRLMELGMTAAEVDECRGFVHRIGVLAEAAVARDHEGIVAM
                     HDVTEGGLATALRELCTAGGHGI"
     gene            1505..2074
                     /locus_tag="VFD74_02780"
     CDS             1505..2074
                     /locus_tag="VFD74_02780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZK48510.1"
                     /translation="MAKEQEPDARSESPGGCAPGAAVQDHYPEESARCYGCGRLNEDG
                     LHVRTCWARDADDHEAAARGAESDETVAHFTPRPEHTALPGIVYGGLIASLIDCHSTG
                     TAAAAVFRAEGRSFDSQPPVRCVTAALHVDFVRPTPLGVTLELRGRVKELKGRKVVVT
                     TTLSALGEVCARGEVVAIRLPPADAADRA"
     gene            complement(2252..2323)
                     /locus_tag="VFD74_02785"
     tRNA            complement(2252..2323)
                     /locus_tag="VFD74_02785"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2289..2291),aa:Val,seq:cac)
     gene            2447..2824
                     /gene="crcB"
                     /locus_tag="VFD74_02790"
     CDS             2447..2824
                     /gene="crcB"
                     /locus_tag="VFD74_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HZK48511.1"
                     /translation="MGIRLLAIVIGGGLGSAVRYLASLLGWRLFGPGFPVATLAVNVA
                     GCLLIGVAFALAETRGAMSPVTRLFVMTGFLGGLTTFSTFALETVDYAHVTSLAVAAL
                     NVLLSTAGGLAAVVAGLWLGRAL"
     gene            complement(2837..3771)
                     /gene="cysK"
                     /locus_tag="VFD74_02795"
                     /pseudo
     CDS             complement(2837..3771)
                     /gene="cysK"
                     /locus_tag="VFD74_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508788.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
     gene            complement(4074..>5183)
                     /locus_tag="VFD74_02800"
     CDS             complement(4074..>5183)
                     /locus_tag="VFD74_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZK48512.1"
                     /translation="PTAIMVGTGKGAENGILIRSGEALERAYKLNAVVLDKTGTLTEG
                     RPVVTDIVLADVVLAEAADRDREAGSVGEVAPPLTEESLLALAASAERGSEHPLGEAI
                     VEKARELGLDLSDGRDFNAVPGHGIEVTVGGARVLLGNLKLMRDRGIEAGTLEDEAER
                     IATEGKTPMFVAVDGRLAGIIAVADTLKPNSAEAVARLQKMGLQVVMMTGDNRRTAEA
                     IGRQVGIDRVLAEVLPEHKAEEVRKLQAEGKVVAMVGDGINDAPALAQADIGIAIGTG
                     TDVAMEASDITLIRGDLMPIATAIDLSRATIRTIRQNLGWAFGYNVALIPVAAGVLYP
                     IFGILLDPMFAAAAMALSSVSVVSNSLRLRRFRAA"
BASE COUNT          794 a         1834 c         1710 g          845 t
ORIGIN      
        1 gatgccgtgg cctccggccg tgcacagctc tctgagggca gtggccaagc caccctcggt
       61 cacgtcgtgc atggcgacga tcccctcgtg gtcacgcgcg acggccgcct ccgcaagcac
      121 cccgatcctg tgcacgaacc cgcgacactc gtctacctca gcggcggtca tccccaactc
      181 catcagccga tcggcaagtt cgcccgcgat gatggcggtg ccctccacgg cgaccccttt
      241 ggtgagcagc acccggtcgc ccggtgccat gttccgcttg tcgaggagac gcgcacgctc
      301 caccgtgccc gccatggtcc ccacgacgat cgggcgaacc accgcttccg tgatctcggt
      361 gtgacccccg cacagggtta tgccgtgccg ccggcaggag tcacagaggt cgaggagggc
      421 gcgtatgacc atggagggcg tggtgcccgg cgggaacagg gccgtcgcga tgaaccaccg
      481 aggagtcgcg cccgaggtcg cgatgtcgtt ggcgttgacg aggaccgcat actctccgat
      541 ggaatccgtg acgagagtga tggggtcgga cttgagcacg agcacctgct cggccgaaat
      601 gtccaccgct gcgacatctt cccccaccgc cggctcgatg aggagggccg ggtcatcggc
      661 gatgaccccc tcgaggtact cgtgcagaag atcgttgggg aacttcccca gtgggagcgg
      721 ccggccgagc atcacgatcc ggctcacctc gacaaggctc tcgatctcga agtcggggcg
      781 gatccgcgga agtccgatga acgacggcgc ggggtctccc ccaagcgccg cacccgtggg
      841 agcaaggagg gcggtgaggc agccggcggc gtgccccgcg gcgacatcga gctggtggtc
      901 tccgaccaca agcgcttcac ccgcgtccac cccgagccgg tcgagaacgt agtggatggc
      961 gtcgggggcc ggcttcggtc ggccggggat gtctcgtgat gcgacgacgt cgaagtcctc
     1021 gatcccgata ccgaagaagt tccgcaggga ggactcgata gagccttggc tgttgcggct
     1081 gacgatggcc agccgcacac cgtcactgcg cagtccccga acgatcgact cggcagcttc
     1141 cgccggcacg gcgagggcgg cggcggcggc ctcgaacctg tcgagcacgg cggagagcct
     1201 cgagcgttcg ccctcgtcgg ccctcgactc gatgaactcg aggatcggct cacctgccgg
     1261 gcacccgatc gcttcgcgga tcgcggcgaa atcgagcgcg cccggcagcg tgagagtgcc
     1321 gtcgaaatcg aagagcactg ctcgtatccg gtacgcccgc tgacccatgc gccctcccgt
     1381 tcgacccgtg ccgcgcagca ggcgacatct gacgtacata ggatatcctg ttgccggggc
     1441 cacgccacgc tcgcgcgcga ccggcgcatc ccccttcggt gtcaccgagc gatgggagcg
     1501 cgtcatggcc aaggagcagg aaccggacgc ccgcagcgag tctcccggcg ggtgcgcgcc
     1561 gggagcggcc gtgcaggacc actacccgga ggagtccgcc cgctgctacg gatgcggccg
     1621 cctcaacgag gatggtctgc atgtccgcac gtgctgggcc cgcgacgcgg atgaccacga
     1681 ggccgccgcc cggggcgcgg agagcgatga gactgtcgcc cacttcactc cacggcccga
     1741 gcatacggcc ttgccgggga tcgtctacgg agggctcatc gcgtcgctga tcgactgcca
     1801 ctccaccggc acggccgcgg ccgctgtctt ccgtgccgag ggccgaagct tcgactcaca
     1861 gccgccggtg aggtgcgtca ccgctgcgct tcacgtcgac ttcgtgaggc ccacgcccct
     1921 gggtgtgacg ctcgaactgc gagggcgcgt caaggagctg aagggccgca aggtggtcgt
     1981 gaccaccacc ctctcggctc tgggtgaggt ctgtgcccga ggtgaggtgg tggcgatccg
     2041 ccttccgccc gccgacgccg cagaccgggc gtgagtgggc gcgcgccgcc gccgccgacc
     2101 acatgtgaac aggcgcgcgc accggcctat ccgccaaccg catgtgagtg ggcgcgcgcc
     2161 atcctgtccg ccgacccatc cgacgcgtcc ccctgacacg cccccctgac gcacgagagg
     2221 ccccgcggtc gcggggcctc tccgatccga gtgggcgata ctgggttcga accagtgact
     2281 tctgccgtgt gaagacagcg ctcttccact gagctaatcg cccctgtcga tggggcgcat
     2341 atactagcaa gccgggccga ccaacgccaa gagcgcggcc atctcggcct ctcctctgct
     2401 accctaccgt ctggcgtgca ctcgaattca cccttggggg tgttccgtgg ggatcaggct
     2461 cctcgcgata gtcataggcg gaggactggg gtcggcagtg cgatacctcg cgtcgttgct
     2521 cgggtggcgg ctgttcggcc caggcttccc ggtggccacg ctcgcggtca atgtcgccgg
     2581 ctgcctcttg atcggcgtgg ccttcgcgct cgcggagacg cgcggcgcga tgtcacccgt
     2641 cacccggttg ttcgtcatga ccggcttcct cggtgggctc accaccttct cgacgttcgc
     2701 gctcgagacc gtggactacg cccacgtgac cagtctcgcg gtcgctgcgc tcaacgtcct
     2761 gctgagcact gcgggcggac tggccgccgt cgtcgcaggc ctgtggctgg gccgagccct
     2821 ctgaccgcag gtcgcgtcac gccagatcgg cgaacaccgg actactcagg tagcgctcac
     2881 cggtgtcggg caagatcacc acgatgagct tgccggccga ctcgggtcgc tgagccaccg
     2941 ccaccgccgc gtggactgca gccccagagg agatgccggc caggatgcct tcctctcggg
     3001 caagtctccg ggtcatggcg aacgcttcgt cgttgctcac ctggacgatc tcgtcgatga
     3061 cgcccacgtc gagcacggcg ggcacgaagc ccgcgccgat gccctggatc ttgtgcggac
     3121 cgggcgcccc tcccgatagg acgggcgagt cggtgggctc taccgccacc gtgcggactc
     3181 cgggcttgcg cgccttcagg accccgccta ctccggtgat ggtgccgccg gtgccaacac
     3241 cgctcacgaa gatgtccacg tcgccgtccg tgtcgagcca gacctcttcg gccgtggtgg
     3301 cgcgatgcac ctcggggttg gcggggttct cgaactgctg cgggacgaag gagccgggtg
     3361 tggccgccga caactcctca gccgccctga tggccccctt cataccttcc gaacccgggg
     3421 tgagcacgag ttccgcaccg taagccttga gtagcgcacg acgctccagg ctcatggtgt
     3481 gtcgggcatg gtgagcagca gtctgtagcc acgtgccgca gcgaccatgg caaggccgat
     3541 gccggtgttg ccggacgtgg gctcgatgat gaccgtctcg ccggggacca tcttcccggc
     3601 ctcctcggcc gccctgatca tgttccagcc gatgcggtcc ttgaccgaac cgcccgggtt
     3661 gaaactctcc agtttcgcca ccactcgggc cccggcgccg ccggtcacct tgttcagctc
     3721 gacgagcggg gtgtgtccga tcaacgccga tacgtcggga gctatgcgca tgtctgcctc
     3781 ctcatcctgc gacgcgggcc cgaagagtgc ccgtccgggc ccggaactcc ctcgaatcgc
     3841 ggcgccttta acactcgaaa ctctatcaag ttactcagct ttactgaccc gagtctagca
     3901 ccttgagcta cgatgtcaag ccctcgcgcc gctggcgcgc ccgcctcagc cgatgcgtct
     3961 cggcctggcg tctccgacat ccgggcatcg ggccgtggct ccgacgtcca ggcatcgggc
     4021 cgtggcctcg aggcgccggc acaggcaccg gcacaggctt cggccggccg gcgtcaagcc
     4081 gcgcggaacc gcctgaggcg cagcgaattg gagaccacgc tcaccgaact cagcgccatg
     4141 gccgcggcgg cgaacatggg gtcaagcagg atcccgaaga tcgggtagag cacgccggcg
     4201 gccaccggga tcagggcgac gttatagccg aaagcccacc ccagattctg ccggatcgtg
     4261 cgaatggtgg cccgactcag gtcgatggca gtggcgatag gcatgaggtc gcctcggatg
     4321 agggtgatat cggaggcctc catggccacg tcggtgccgg tgccgatggc gatgccgata
     4381 tccgcctgag ccaaagccgg agcgtcattg atgccgtcgc cgaccatggc cacgaccttc
     4441 ccctcggcct ggagtttgcg cacttcctcg gccttgtgct cggggagcac ctcggccagg
     4501 accctgtcga tgcccacctg ccgccctatg gcctcggcgg tgcggcgatt gtcgccggtc
     4561 atcatgacca cctgcaggcc catcttctgc agcctcgcca cggcctccgc ggaattgggc
     4621 ttcagcgtgt ccgccaccgc tatgatgccg gcgagacgac catccacggc cacgaacatg
     4681 ggagtcttgc cctcggtggc gattcgttcc gcctcatcct cgagggtgcc cgcctcgatg
     4741 cccctgtcac gcatgagctt gaggttccct agaagcacgc gggcgccccc gaccgtcact
     4801 tcgatgccgt ggccgggcac cgcgttgaag tctcgtccat cggacaggtc gagcccaagc
     4861 tcgcgcgcct tctcgacgat ggcttcgccc agggggtgct ccgagccgcg ctcagccgag
     4921 gcggccagcg cgaggagcga ctcctctgtg aggggcggag cgacctcccc gacggagccg
     4981 gcttcgcggt cccggtcggc cgcttccgcg agcacaacat ccgcgagcac gatgtcggtg
     5041 accacaggcc tgccctccgt gagggtgccc gtcttgtcga gcaccacggc gttcagcttg
     5101 taggcgcgct ccagcgcttc gccggagcgg atgagaatgc cgttctcggc ccccttgccc
     5161 gtccccacca tgatggccgt agg
//
LOCUS       DASRMJ010000093         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_444417, whole genome shotgun sequence.
ACCESSION   DASRMJ010000093 DASRMJ010000000
VERSION     DASRMJ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444417"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFD74_02805"
     CDS             complement(<1..353)
                     /locus_tag="VFD74_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48513.1"
                     /translation="MFPTVVLVVALGCALLAPMGLNVGMRRSQYQVAQLQQRMDDLVA
                     ERSTLKAEQASLSSTQRVKDTADRLGMVTPAEIGFIDLAGSAGSTQPTLAASAAASAG
                     DRVGDGHVVASTSQET"
     gene            complement(650..1624)
                     /gene="rsmH"
                     /locus_tag="VFD74_02810"
     CDS             complement(650..1624)
                     /gene="rsmH"
                     /locus_tag="VFD74_02810"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZK48514.1"
                     /translation="MMTAVAYSLEFEAEMAMAHVPVLTNELLDVLDLEPGSRVVDCTF
                     GAGGHAFEVMRRLDSSGLLLACDRDPVAQGYFDEWKRTVQTPTRFYRGDFADTLQQLE
                     RSGQSVDRIYMDLGVSSMQIDTEERGFSYSYDAPLDMRMDPDLSLTAADILNEWDQER
                     ICWVLREFGEERFASRIARSIVRRRLQAPYRRTSELVDTVKASIPAPARFGSRNPARR
                     VFQALRIAVNGELDSLRRALPQALSLLEPGGVAAVISFHSLEDRIVKEFFAAQVHPCT
                     CPPDFPICVCGKEPTMEMITGKPITPTIREIESNPRSSSAKLRAARKL"
     gene            complement(1624..2070)
                     /locus_tag="VFD74_02815"
     CDS             complement(1624..2070)
                     /locus_tag="VFD74_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48515.1"
                     /translation="MEATDTSPIFFTDRYEHSVDDKGRVTLPAQYRRYFPADSIAYFV
                     PAGHGEPCIRVFPLESWTQYDQKYLEQLNEFEAKDVSRMIRNLYSGIKKVTVDKAGRV
                     LLPDAAVKTLGLSGKVLMAGHRDHFEIWDPQAFEEFVQSECEEDAS"
BASE COUNT          418 a          751 c          717 g          404 t
ORIGIN      
        1 gtctcctggg atgtcgaggc cacgacgtgc ccgtcgccca cgcggtcacc ggcggaggcg
       61 gcggcggacg cggccagagt gggctgcgtc gaaccggcac tccccgcaag gtcgatgaag
      121 ccgatctcgg cgggagtgac catccccaga cggtcggcgg tgtccttcac ccgttgcgtg
      181 gacgagaggg aggcctgttc ggccttgagc gtcgaacgtt ccgcaaccaa gtcgtccatt
      241 cgttgctgca actgggccac ctgatactgc gagcggcgca tacccacgtt gagtcccatc
      301 ggagcgagga gtgcgcaccc gagggccacc accagcacca ccgtcggaaa caaacggacc
      361 cgcgagtcgc gaacgacccg aagagtcggc tgccgaaccg gacttctttc ggccgatggt
      421 cttcgctcgg ccgaacgcgc tcgctcagcc gacgacctgc cccggacctc gggggcttcg
      481 tgatgggagt ttgtctccca ggaggcctcc cggcccgcgt aggagcgtgc tttggcgaca
      541 gccgttccgg ttcgcccagc cacgcgctcc gcactcctgc gggccgcggc ggaccgcgtc
      601 tcgtcgtagc tccaccgagg gtggtcgtag gagcgcgcag ccgcacccat cacaacttcc
      661 tagcagcacg gagtttcgcc gaactggagc gaggattcga ctcgatctcg cgaatcgtgg
      721 gtgtgatggg cttgcccgtg atcatctcca tcgtcggctc cttgccgcac acgcatatgg
      781 ggaagtccgg cgggcaggtg cacgggtgga cctgggcggc gaagaactcc ttgacgatgc
      841 ggtcttcgag cgaatgaaag ctgatcaccg ccgccacccc tcccggctcg agaagcgaga
      901 gggcctgcgg aagagcacgc cggagactgt ccagttcgcc gttgacggcg atgcgaagtg
      961 cctggaacac tcgcctggcc gggttccttg agccgaatcg ggccggagcg gggatcgagg
     1021 ccttgacggt gtcgaccagc tcactcgtgc gccggtaggg cgcctgcagg cgccgacgca
     1081 cgatggagcg ggcgatacga gaggcgaacc gctcctcgcc gaactccctc agcacccagc
     1141 agatgcgttc ctgatcccac tcgttcagga tgtcggcggc ggtcaggctc agatcggggt
     1201 ccatgcgcat gtcgagaggt gcgtcgtagc tgtacgagaa gcctcgttcc tccgtgtcga
     1261 tctgcatgct gctcacgccg agatccatgt atatgcggtc cacagactgc cccgagcgct
     1321 ccaactgctg aagcgtgtcg gcgaaatcgc cgcggtagaa ccgggtggga gtctgcaccg
     1381 tgcgtttcca ctcgtcgaag tagccctgag ccacgggatc gcggtcgcat gcgaggagga
     1441 ggcccgagga atccagccgc cgcatcacct cgaaggcgtg tccgcccgct ccgaaggtgc
     1501 aatcgaccac ccgagagccc ggttcgagat ccagcacatc caagagctcg ttcgtgagta
     1561 cgggcacgtg ggccatggcc atctccgcct cgaattccag tgagtacgcg actgcagtca
     1621 tcatcaggac gcgtcctcct cgcactcaga ctgcacgaac tcctcgaagg cctgaggatc
     1681 ccagatctca aagtgatccc ggtggccagc catgaggacc ttcccggaca accccagggt
     1741 cttcacggct gcgtccggca ggagcacccg ccccgccttg tcgacagtga ccttcttgat
     1801 gcccgagtag agattgcgga tcatccgaga cacatccttc gcctcgaact cgttcaactg
     1861 ttcgagatat ttctggtcgt actgtgtcca gctctcgagc gggaacaccc tgatgcaggg
     1921 ttccccgtga ccggccggaa cgaagtaggc gatcgagtcc gcggggaagt agcgccggta
     1981 ttgcgcgggc aaggtgaccc ttcccttgtc gtccacgctg tgctcgtacc ggtcggtgaa
     2041 gaagatgggc gatgtgtccg tcgcttccac agtgtgccca gactaatccc atccactccc
     2101 aaatgcaacc cttttgttgc acttcgccat catttcgtcc cacatcggcg ctttgcaggg
     2161 attcgtggac ccctcggaac aacacccaga cgaccttcag aggccgaaat cgccccgcgt
     2221 acgcaatgca tactgcaaaa gcatacgcat gcagctcatg ttgcgcatcg gcgacagcgc
     2281 gggatgtgag
//
LOCUS       DASRMJ010000094         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_368206, whole genome shotgun sequence.
ACCESSION   DASRMJ010000094 DASRMJ010000000
VERSION     DASRMJ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368206"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFD74_02820"
     CDS             complement(<1..450)
                     /locus_tag="VFD74_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZK48516.1"
                     /translation="MDAHDSTADQLETIELGDYLRVIRERKWIIIAAVAVVAAAALLF
                     SLLSTPKYRASSQLLYKVSNLDVTLFGSRVFEVRDLNRELQTGAGMVRLNTVADAVAQ
                     ELGSGYTTEQLLGMVDVRPDAQTNFIDITATSVHPVEAADIADAFAQK"
     gene            complement(779..1942)
                     /locus_tag="VFD74_02825"
     CDS             complement(779..1942)
                     /locus_tag="VFD74_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZK48517.1"
                     /translation="MGTFIDESGFLAAGRALRGARMSAGQTLEQVSRALSIRVLYLEA
                     LERGEFGRVLGPTYAESMIVRYAVHLGLDAEALLTGAAKPHVGPGDMGARGTEPGTSA
                     GKIPETAPGTAPGTVPGPASATPAGVRPISPTGPAAGAPPVAAPSSAPSSVPAPAVVP
                     KAPVPTPKSAPTPDFFSPTPRGEKHSPTPTASRSALDVPRRSRRGSGGSTTARRGGSR
                     LLRVAVLGVLVIGLVAVTFFGASELGLFSIFDGDSVGTADSSSTTAVSETGDTTPTTD
                     GSVTPSTSAPTSTTTLMASTTTTEAQAAVPFSLTVKAKEDVWVEFRDAVSGEALHVGM
                     IAKDESVTLSAEGSVNAVVGKPGALTLQIDGRSVQPPNSFRWLVTSTGVEDRP"
     gene            2188..3234
                     /gene="rsgA"
                     /locus_tag="VFD74_02830"
     CDS             2188..3234
                     /gene="rsgA"
                     /locus_tag="VFD74_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZK48518.1"
                     /translation="MIDSRARLALEPLGFDDRLEKLFRPLLAKGLLIGRVARVDRGSS
                     YLLTAGDAVRADTAPRLRRDPTGLAPPTVGDWVAFRVDEDGRTEIEHILERTSTFARG
                     DPAHPGASQILAANIDTVFLVHSLTRTLNLRRLEREFVLVYESRAMPVVVLNKADLVP
                     EADAARARAAAETAALGRPVHVVSAITSLALDELEPYTRGNRTIALVGASGVGKSTLV
                     NRLVGAAVQRIGAVRESDEKGRHTTVARELIVLPDGGSLIDTPGLRSLAMKEADVGLA
                     AAFADIETLAASCRFRDCSHLQEPGCALRDAVEQGTLPAGRLAGYFKLRDELAADATQ
                     QLRNERARGEGRRR"
BASE COUNT          577 a         1156 c         1166 g          531 t
ORIGIN      
        1 cttctgcgcg aaggcgtctg cgatgtcggc cgcttcgacc gggtgaacgc tggtggccgt
       61 gatgtcgatg aagttggtct gcgcatcggg gcgaacatcg accataccta gaagttgctc
      121 ggtggtgtag ccggaaccca actcctgggc gaccgcatcc gcaacggtgt tgaggcgcac
      181 cattcccgcg ccggtctgca gctcgcggtt caggtcgcgc acctcgaaga cgcgggagcc
      241 gaacagcgtg acgtccaggt tcgagacctt atagaggagc tgggacgaag cccgatactt
      301 gggagtgctt agaagagaga acaagagtgc cgctgccgct actaccgcga ccgccgcgat
      361 gattatccac ttgcgctctc ggatgactcg cagatagtca ccgagttcga tggtctcgag
      421 ctggtccgcg gttgagtcgt gggcatccat gaggggctag tctagtgtat ggaccgcaca
      481 acatacaagc gatcgaggcg tgacgcggta gggaagcccc cgcgctgctc cgcttccgct
      541 tcccgcccgg cttccgagcg gacggcgcag gagggtgcga cgtgtggtcc ccgtcgtgac
      601 ccgcggagca cgctagcagg ctgctgaaga gggacgcttc gcctccagag cgcgatgggc
      661 gagtgcgaga caaggacgca ggaagcgcac gtagcggccg ctacgtgcgc gaccgaggac
      721 gccgtatcgc gccgcccagt gcgttctggg ggccaaggct tcgcttttca gcagcctcct
      781 atggccggtc ttcgacgccg gtggaagtca caagccagcg gaaggagttc ggaggttgca
      841 cgctccgacc atcgatctgg agcgtcagcg ccccaggctt gccgaccaca gcgttcacgg
      901 acccctccgc ggagagcgtc accgactcgt ccttggcgat catccccacg tgcagtgcct
      961 cgccggacac cgcgtcacgg aactcgaccc acacgtcctc tttggccttg acggtcagtg
     1021 agaaggggac ggccgcctgg gcttctgtgg tggtggtcga agccatgagc gtcgtggtcg
     1081 aagtcggagc gctcgtgctc ggcgtgacag atccgtcagt cgtgggcgtg gtatctccag
     1141 tctcggagac ggcggtggtg gaactcgagt ccgcggtgcc gacagagtct ccgtcgaaga
     1201 tcgagaacag gccgagctcc gaagccccga agaaggtgac ggcgacgagg ccgatcacca
     1261 ggacgccgag gacggctacc ctcagcagcc gagacccgcc gcggcgggct gtggtcgagc
     1321 cgcccgaacc gcgccgggac ctcctcggca cgtcgagagc gctgcgggag gcggtcggcg
     1381 tcggggagtg cttctcgccg cgcggcgtcg gactgaagaa gtcaggcgtc ggcgcagact
     1441 tgggcgtggg cacgggcgcc ttgggcacaa ccgccggcgc aggcactgag gacggtgcgg
     1501 acgatggagc ggcgacgggc ggggcgcccg ctgcgggccc ggtcggcgat atcggacgga
     1561 ctcccgccgg tgtggcggag gccggtccgg ggacggttcc tggggcggtt ccgggggccg
     1621 tttctgggat ctttccggcg ctcgttccag gctccgttcc ccgcgcgccc atgtccccgg
     1681 gaccgacgtg cggcttggcc gcccccgtga gcagcgcctc cgcatccaat ccgagatgca
     1741 ccgcataccg aacgatcatg ctctcggcgt aggtcgggcc cagcactcgg ccgaactccc
     1801 cgcgctccaa agcctccagg tagagcactc ggatgctcag cgctcgggac acctgctcaa
     1861 gcgtctgccc cgctgacata cgcgcgcccc ggagcgcgcg acccgcagcc aggaacccgc
     1921 tctcatcgat gaatgtgccc attccgcccg tctcctcgtc gccgtatcga taccagttcc
     1981 gcggaacaca gctcaatctt cccacatccg gggctccggc gggggcggct gttgacgccc
     2041 ccatggcccc gtactatgct gccagtgtcc aagaccccgc tccgccgcgg tcaacgccga
     2101 gaggcgacat ccccgtccgg gaagacatct cgcacccagg gagtcacgtg caccagacca
     2161 catccccaag caccggccgc aacacggatg attgactccc gggcgcggct cgcgcttgag
     2221 ccgctcggct tcgacgatcg actcgagaag ctctttcggc ctcttctcgc caagggactg
     2281 ctgatcggac gcgtggcccg cgtcgaccga gggagcagct acctgctcac cgccggtgat
     2341 gccgtccgag ccgacaccgc ccctcgcctg cgccgggatc ccacgggact cgcgccaccc
     2401 accgtcgggg actgggtggc attccgggtg gacgaggacg ggcggaccga gatcgagcac
     2461 atattggaga ggaccagcac cttcgcgcga ggcgatcccg cgcacccggg tgcatcccag
     2521 atcttggccg ccaacatcga tacggtcttc ttggtccact ccctcacgcg caccctcaac
     2581 ctgcgccggc tcgaacggga attcgtgctg gtgtacgaga gcagagcgat gccggtggtc
     2641 gtcctgaaca aggcggacct ggtcccggag gcggatgcag cccgagcgcg ggccgcagcg
     2701 gagaccgcgg ccctcggacg accggtccac gtggtaagcg cgatcaccag cctggcactc
     2761 gacgaactcg agccgtacac gcgcggaaac cggacgatcg ccttggtcgg ggcatccggc
     2821 gtgggcaagt ccacgctggt gaaccgcctg gtgggcgccg cggtccaaag gatcggagcg
     2881 gtgcgtgaga gcgacgagaa gggccgacac accacggtgg cccgagagtt gatcgtgctc
     2941 ccggacggcg gttcgctcat cgatacgccc ggcctgcgct ctctcgcgat gaaggaggcc
     3001 gatgtcggac tcgcggccgc cttcgccgac atcgagactc tggcggcgtc ctgccgtttc
     3061 cgtgactgca gccacctgca ggagcccggc tgtgcgcttc gcgacgccgt cgagcaaggg
     3121 acgctaccgg cgggacgcct cgccggttac ttcaaactgc gcgacgaact cgccgcggat
     3181 gccacccagc aactgcggaa cgagcgggcg cgtggagaag gacgcaggcg ctaacttgcc
     3241 gcccagcctc tcgtctgagg aagatagatg accgggatcc gtagcgccag ccgacacatg
     3301 gggttcctgc gcgcagtcac gccgctcgtc ctgggcgcgg cgatgctgat gatgctcgcc
     3361 accctcccgg atgtcgtcaa gaaggatgag gacagccggc tcaccttccg acccgccgcc
     3421 gtggtggaag
//
LOCUS       DASRMJ010000095         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_332350, whole genome shotgun sequence.
ACCESSION   DASRMJ010000095 DASRMJ010000000
VERSION     DASRMJ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332350"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /gene="purD"
                     /locus_tag="VFD74_02835"
     CDS             complement(<1..847)
                     /gene="purD"
                     /locus_tag="VFD74_02835"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZK48519.1"
                     /translation="MAVDVLVVGGGGREHALVSGLKRSARIGRVFCAPGNAGIARDAT
                     LVPIGADDVAALADFAERESIGLTVVGPEAPLVAGIADVFTERGLVVFGPPRAGALLE
                     GSKAFAKEVMAAAGVPTGGAEAFTDYAAASSYLRAHGAPVVVKADGLAAGKGVTVAMS
                     EEEADRALRECFVERRFGDAGGTVLLEEFLVGEELSLLAIVSGDKVLPLAPAQDYKRI
                     FDGDLGPNTGGMGCYSPVPSVSPALYDELVSTVIRPTVADLAARGIDFRGVLYAGIIL
                     TAAGPR"
     gene            complement(1016..2290)
                     /locus_tag="VFD74_02840"
     CDS             complement(1016..2290)
                     /locus_tag="VFD74_02840"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZK48520.1"
                     /translation="MPVTVVLGAQWGDEGKGKVVDLLSQDADMVVRYQGGNNAGHTIK
                     NERGDFALHLIPSGILNPDTVAVIGNGVVIDPTALRAEIDELEGRGVSTANLKISMNA
                     HLIMPYHILLDKVGENVLGRRKIGTTRRGIGPAYADKAARVGVRVQDLLDAKIFHQKV
                     RAAMETKSVLLRLVYDQNVAGLESECDRYLSMAETLRPYITDASLLVWQAAKAGKRVL
                     FEGAQGTMLDIDHGTYPFVTSSNPVAGFAAVGSGIGPTMIDEVIGVCKAYATRVGEGP
                     FPTELHDERGRILRDVGHEFGTTTGRERRCGWLDLVALRYAVRVNGMTGLVITKLDVL
                     NQFDEIEVCTEYRHGSEVLKEMPRHQSVFHKVEPVYRRFPGWKADISGARTLSDLPKA
                     ARDFLAYISEHTETPLTLVSVGPRREETILGA"
BASE COUNT          396 a          829 c          773 g          372 t
ORIGIN      
        1 cccggggtcc ggcggcggtc aggatgatgc ccgcatagag cactccccgg aaatcgatgc
       61 ccctggcggc caagtctgcc acggtgggcc ggatgacggt gctcaccagc tcgtcgtaga
      121 gcgcggggct gacgctgggg acgggagagt agcaccccat cccgccggtg ttggggccca
      181 ggtcgccgtc gaatatccgc ttgtagtcct gcgcgggggc caggggcaag accttgtcgc
      241 cactgacgat ggcgagcagc gacagctctt cccccacgag gaactcctcg agcagcacgg
      301 tgccgccggc atcgccgaag cgccgctcga cgaagcactc ccgcaaggca cggtccgctt
      361 cctcttcgct catggcgacg gtcacacctt tgccggccgc cagaccatcc gccttgacca
      421 ccaccggagc gccgtgagcg cgcaggtagg aggaggcggc ggcgtagtcg gtgaaggcct
      481 cggcgccgcc cgtgggaacg ccggcggcag ccatcacctc cttcgcgaag gccttcgagc
      541 cctcgagcag cgcccccgcg cgcggcgggc cgaacaccac caggcctcgc tcggtgaaga
      601 catcggcgat gccggccacc agcggagcct cgggaccgac gacggtgaga ccgatcgatt
      661 cgcgctccgc gaagtccgcc agcgccgcga cgtcgtcggc gccgatggga accagggtgg
      721 cgtcacgggc gatgccggcg ttcccaggtg cgcagaacac gcgaccgatc cgagcgctcc
      781 gcttcaggcc cgagaccagg gcgtgctcac ggcctccacc gccgacaacg agtacgtcaa
      841 cagccatcgc gagcgctccc cttcctcgag gtgcccgcgc cagcgggcgg cagggctggg
      901 caccatcttg aacatgcgcc cattctactc accggcgccc ctggagtcga cccgactgca
      961 cttgatgtgt caggcggatg tcccggggcc ccgcccgacg actgagacgc gccggtcagg
     1021 ctccgaggat ggtctcctcg cggcgcggcc cgacggagac gagtgtcagg ggcgtctctg
     1081 tgtgttcgga gatgtacgcg aggaagtccc gcgccgcctt cggcagatcc gagagagtgc
     1141 gggcgccgga gatatcggcc ttccagcccg ggaatcgccg atagaccggc tccaccttgt
     1201 ggaacaccga ctggtgcctc ggcatctcct tcaggacttc ggagccgtgg cggtattcgg
     1261 tgcacacctc gatctcgtcg aactggttca gaacatccaa cttggtgatc acgagacccg
     1321 tcatgccgtt gacccgcacc gcgtagcgca gcgccaccag gtcgagccac ccgcagcggc
     1381 gctcccgtcc ggtggtggtg ccgaactcgt gcccgacatc gcgcaagatc cgcccgcgct
     1441 cgtcgtgcaa ctccgtgggg aagggcccct cgcccacccg ggtagcgtac gccttgcaga
     1501 ccccgatgac ctcgtcgatc atcgtcggcc cgataccgga gcccaccgcc gcgaaaccgg
     1561 ccaccgggtt agaggacgtg acgaacggat acgtgccgtg gtcgatgtcg agcatcgtgc
     1621 cttgagcacc ctcgaacagc acccgcttgc cggccttggc cgcctgccag acgaggagtg
     1681 aggcgtcggt gatatagggc cgcaacgttt cggccatgct caggtagcgg tcgcactcgg
     1741 actcgaggcc cgccacgttc tgatcgtaca cgagccttag gagcaccgac ttggtctcca
     1801 tcgccgcccg caccttctgg tggaagatct tggcgtcgag caggtcttgc acgcggacgc
     1861 ccacccgcgc cgccttgtcc gcgtaggctg gaccgatgcc gcgacgggtg gtgcctatct
     1921 tgcgacgccc gaggacgttc tcccccactt tgtcgagcag gatgtggtag ggcatgatca
     1981 ggtgcgcgtt catggagatc ttcaggttgg ccgtggagac gccgcgaccc tcgagttcgt
     2041 cgatctcggc acgaagcgcg gtgggatcga tcaccacacc gttaccgatg acggcgacgg
     2101 tgtccgggtt caagatgccc gaagggatca ggtggagcgc aaagtcgccg cgctcgttct
     2161 tgatggtgtg gccggcgttg ttgccgccct gataccgcac caccatgtcc gcgtcttgcg
     2221 acagcaggtc gacgaccttt cccttgccct catcacccca ctgcgcaccc aagacgaccg
     2281 taaccggcac cgagaccctc cccgaacgtt cagtactatc gaccacggcg accgagggcc
     2341 gccgtgggag cctggaagag tgtacaccag
//
LOCUS       DASRMJ010000096         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_479296, whole genome shotgun sequence.
ACCESSION   DASRMJ010000096 DASRMJ010000000
VERSION     DASRMJ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479296"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFD74_02845"
     CDS             complement(<1..463)
                     /locus_tag="VFD74_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZK48521.1"
                     /translation="MPATTGVTSPAAEKSLSGHRAAVRRGPGLGMRVLLAAAGLVAWV
                     FLYRALEPFSRWAAFSVFGLDAATAVGAAVQFFLYDAPKVLMLLLLVVFAVGIVRTFF
                     TPERTRALLAGRRESVGNVLGAALGIVTPFCSCSAVPLFIGFVTAGVPLGVT"
     gene            complement(463..711)
                     /locus_tag="VFD74_02850"
     CDS             complement(463..711)
                     /locus_tag="VFD74_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZK48522.1"
                     /translation="MEIKVMGTGCAKCNQLYAVTRQAAESAGLDAAVVSKVEDIDEIL
                     AHGVMMTPALVIDGVVKSTGKVPAEADIVAWIATALEG"
     gene            complement(762..1220)
                     /locus_tag="VFD74_02855"
     CDS             complement(762..1220)
                     /locus_tag="VFD74_02855"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZK48523.1"
                     /translation="MRWGRVRELASMFKALGDETRLEMLALLAQRDELCVCRFEEALG
                     ITQSKASRHLRYLLNAGLVTDRRDAVWVHYRIAPDLGEEQAALIDALPRILGEARMAA
                     TLARLLPGDVARESPSDAACEAGSGVACEAGGRVGEEVTACAPAVTRASK"
     gene            complement(1337..1639)
                     /locus_tag="VFD74_02860"
     CDS             complement(1337..1639)
                     /locus_tag="VFD74_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZK48524.1"
                     /translation="MMDWGGFGGFGGMGVIWFFAMAAFWVLILVGVVLVIKALVGGGH
                     HTSTHSHVSPVGQQAAPPAPAAPASQSDALRILQERYARGELDRDEFLQRKGDLTS"
     gene            complement(1799..2935)
                     /locus_tag="VFD74_02865"
     CDS             complement(1799..2935)
                     /locus_tag="VFD74_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015106407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZK48525.1"
                     /translation="MRRDTSAVHTGVAKDATYNSVTTPIYPSSTFMFDRVGLHRGYDY
                     TRSGNPTRSALEENLAALEGGTFARATCTGMAAVTSALLLLSQGDHVVAGHDIYGGTY
                     RLFETVLPRFGLNFTFVDMCDEAAVRSAIRPETRMLWIETPSNPLLNIVDIAAMVAIA
                     REHHLVTVADNTFLSPYFQKPLDLGVDVVLHSTTKYLNGHSDVVGGVVITSRQDLAEQ
                     LGYLVNAMGLANSPFDAWLVLRGVKTLGPRMRAHEAGAGAVARFLSAHPKSLRTYYPG
                     LEGHPGHELAKRQQTGFGGMVSFDLDVDALSPDAFCSRLRVFVLAESLGGVESLIEQP
                     WTMSHVGMSEEARVAAGISPGTMRLSVGIEDPDDLVEDLAQALR"
     gene            3113..3553
                     /locus_tag="VFD74_02870"
     CDS             3113..3553
                     /locus_tag="VFD74_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK48526.1"
                     /translation="MITRYLLGAAMFAVGMTAAIQPPINAALAKRTGGLESATISFAV
                     GTAVLLLLTVVLGNGSLWAARGAPAWQWTGGLVGALFVWATIVMVPRLGAAGLIAGVV
                     AGQLVGGVVIDRFGLFGLPQTSISSTRVAGLVLLLVGGALVVRR"
     gene            complement(3581..>4410)
                     /locus_tag="VFD74_02875"
     CDS             complement(3581..>4410)
                     /locus_tag="VFD74_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZK48527.1"
                     /translation="EPVIITAALVGAEVTLEQLPHLPVTPAQIADAAASAAAAGAVII
                     HLHVRDPGGRPTQDAAVFEETILAIRERVDVIVQVSTGGAMGMTAEERLQPVTRLEGR
                     ARPEMASLTTGSCNFGNEVFLNPLPEVEVFARAMVERGIKPEIEVFEAGMIETALRLS
                     RAGVLPESLHFNLVMGVPGAVPATARHLLNLVEQLPPSSTWTVSGIGRGQLVMAAMGL
                     VMGGHVRTGLEDNIYFRHGEPAESNAQLVRRVVRLANEFDRPVARVAEARELLGLAP"
BASE COUNT          762 a         1504 c         1456 g          688 t
ORIGIN      
        1 gggtcacgcc gagcggcacc ccggcggtga cgaacccgat gaagagaggc actgccgagc
       61 aggagcagaa gggcgtcacg atgccgaggg ccgcgccgag gacgttgccc accgactcgc
      121 ggcggcccgc cagaagcgcc cgcgtgcgct cgggagtgaa gaacgtacgg acgattccca
      181 ccgcgaaaac cacaagcagc aggagcatca gcactttggg ggcgtcgtag aggaagaact
      241 gaacggcggc gcctacagca gtcgcggcgt ccaggccgaa caccgagaac gcggcccacc
      301 gcgagaaggg ttccagcgcc cggtagagga acacccaagc gaccaaccct gccgcggcca
      361 gaagcacacg catgcccagg ccgggacccc ggcgcaccgc ggctcggtgg ccggacagcg
      421 acttctcggc ggcgggcgaa gtgacgcccg tggtcgccgg catcatccct ccaaggctgt
      481 cgcgatccag gcgacgatat cggcctcggc gggcaccttg cccgtcgact tgaccacccc
      541 atcgatcacg agcgccgggg tcatcatcac cccgtgcgcc aggatctcgt cgatgtcctc
      601 gaccttcgag acgaccgccg cgtcgagacc ggcgctttcg gcggcctgcc gcgtgacagc
      661 gtagagttgg ttgcatttcg cgcaacccgt gcccatcacc ttgatctcca cgatccctcc
      721 tgccgcttgt tccacacccg cccggccgcg ccggcgggct cctacttcga cgcccgagtg
      781 accgccggtg cgcaggccgt cacctcttca cccactcggc cgccggcttc gcacgcaact
      841 ccgctgccgg cttcgcaggc ggcgtccgag ggggactcgc gggcgacatc gccgggcagc
      901 aggcgggcaa gggtggcggc catgcgcgcc tcgcccagga tgcgcggcaa cgcatcgatc
      961 aacgcagctt gctcttcgcc aagatcgggg gcgattcggt agtgcaccca gaccgcgtcc
     1021 cggcggtcgg tcacgagacc ggcgttcagc aggtagcgaa gatggcgcga agccttggat
     1081 tgagtgatgc cgagagcttc ctcgaagcga cagacgcaca gctcgtctcg ctgcgccagc
     1141 agcgcgagca tctcgagccg cgtctcgtca ccgagcgcct tgaacatgga ggccagttcc
     1201 cgcacgcgtc cccatctcat ccgtataacc gtttacgcgg ttataagata gcgccttcgg
     1261 ggagcgcgtg caagtgcggg tggaaggcca cacgcaagcg tggacggggc cggacgtgcg
     1321 tccggccccg agagcctcag gaggtgaggt cccctttgcg ctgcaggaac tcgtcgcggt
     1381 cgagctcgcc gcgcgcgtat cgttcctgaa gtatgcgcag agcatccgac tgagaagcag
     1441 gcgccgcggg cgccggcgga gccgcctgct gaccgaccgg ggacacatgc gaatgcgtcg
     1501 aggtgtgatg tccgccgccc acaagcgcct ttatcaccaa gactactccg accaagatca
     1561 acacccagaa cgccgccatc gcgaagaacc agatcacgcc catacctccg aaacctccaa
     1621 atccgcccca gtccatcatc agtcgaccct ccaccgtccg cgcgcgcggg ccgtcgaacc
     1681 cgtcgcgact cttcgcgggc gactggccgt cgccgcgcct cgaacatgag tattcccgaa
     1741 aagtgtgaaa cgcatgtgaa ggttggcggc cgcgagcagg ttgggccgcc gcacgggatc
     1801 agcgcagggc ctgagccaga tcctccacca ggtcgtcggg atcctcgatg cccaccgaga
     1861 gccgcatggt gccgggcgag atgcccgcgg ccacacgcgc ctcctccgac ataccgacgt
     1921 ggctcatcgt ccacggttgc tcgatcagcg actccacgcc ccccagcgac tccgccagaa
     1981 cgaacacgcg caatcgcgag cagaaggcgt cgggggagag cgcatcgacg tccaggtcga
     2041 agctgaccat accgccgaag cccgtctgct gccgcttggc cagttcgtga ccgggatggc
     2101 cctcgagccc ggggtagtag gtgcgcaaag acttcggatg tgcgctcaag aacctggcca
     2161 cggctccggc tcccgcttcg tgcgcccgca tccgcggccc gagcgtcttg acgccgcgga
     2221 ggaccagcca agcatcgaac ggggagttcg ccagccccat ggcgttgacc aggtagccca
     2281 gttgctcggc caggtcttgg cggctggtga tgacaacgcc gcccaccacg tcgctgtggc
     2341 cgttcagata cttggtggtg gagtggagca ccacgtcgac ccccaggtca agcggcttct
     2401 ggaaataggg actgaggaag gtgttgtcgg ctacggtcac cagatggtgt tcccgcgcga
     2461 tcgccaccat cgcggcgatg tccacgatgt tgaggagtgg gttcgacgga gtctctatcc
     2521 acaacatgcg cgtctccgga cggatagcgg aacggaccgc tgcttcgtcg cacatgtcta
     2581 cgaaggtgaa gttgaggccg aaccgcggta gcaccgtctc gaacaggcga taggtgccac
     2641 cgtagatgtc gtgcccggcg acgacgtggt cgccctgtga caggagcagc agggcggagg
     2701 tgacggccgc catgcccgtg cacgtggcgc gggcgaacgt gcccccctcc aaggcggcca
     2761 ggttctcttc gagggccgaa cgcgtggggt tgccgctccg ggtgtagtcg tagcctcgat
     2821 ggaggcccac tcgatcgaac atgaaggtgg aagaaggata gatgggtgtc gtcaccgagt
     2881 tgtaggtggc gtctttcgct acgccggtat ggacggcgga cgtatctcgt ctcatggtct
     2941 atcctccgcg atgtggttgc agaggattat agggaaatct tgaccagcgt gccccgcacc
     3001 gtcaggatag gctgatcagg accgcccgca gaagtggagg gagagctcgc ggcagtcgcg
     3061 ctcaggagac ccacaccctc cgacacccac gaccggaagg gaactttcac acatgatcac
     3121 gcgctacctt ctcggtgcgg ccatgttcgc ggtgggcatg accgcagcca tccaaccccc
     3181 catcaacgcc gccctggcca agcgcaccgg cggactggaa tccgcaacga tcagtttcgc
     3241 cgtcgggacc gcagtcctgt tgctgctcac cgtggtgctg ggcaacggca gcctgtgggc
     3301 cgcccgtggt gctccggcgt ggcaatggac cggcggcctg gtcggcgcgc tcttcgtgtg
     3361 ggctaccatc gtgatggtgc cacgcctcgg tgccgccggc ctcatcgcgg gcgtggtggc
     3421 cggccagctg gtgggtggag tggtgatcga ccgtttcggc ctcttcggtc tgcctcagac
     3481 gagcatctcc tccacccgcg tggccggcct ggtgctgctg ctggtgggcg gggctttggt
     3541 ggtgcgccgc taggaactcg tttcgcgatg aagcccgccg tcagggcgcg aggccgagca
     3601 actcacgggc ctccgcgact cgcgccacag gccggtcgaa ctcgttcgcc aaccggacca
     3661 ccctccggac gagttgagcg ttgctctcgg ccggctcgcc gtggcggaag tagatgttgt
     3721 cttcgaggcc ggtgcgcacg tgaccgccca tgaccagtcc catcgccgcc atcacgagct
     3781 gcccgcgacc gatgcccgac acggtccacg tcgatgacgg gggcagctgc tccaccagat
     3841 tcaacaggtg gcgtgccgtg gcgggcacag ctccggggac gcccatcacc aggttgaagt
     3901 ggagtgactc gggcagcacg cccgcgcgcg acaagcgcag ggccgtctcg atcatccccg
     3961 cttcgaacac ctcgatctcg ggtttgatgc cgcgttcgac catggcccgc gcgaagacct
     4021 ccacctcagg cagagggttc aggaacactt cgttgccgaa gttgcagctg cccgtggtga
     4081 gcgacgccat ctcggggcgc gcgcgcccct cgagtctggt gaccggctgc agacgctcct
     4141 cggcggtcat gcccatggcc ccgcccgtgg agacctgcac gatcacgtcg acccgctcgc
     4201 gtatagccag gatggtctcc tcgaacaccg ccgcgtcctg ggtgggcctg ccaccgggat
     4261 cgcgtacgtg aagatggatg atgacagccc cggccgccgc cgccgaggcg gcggcgtcgg
     4321 cgatctgtgc cggcgtcacc gggaggtgcg ggagctgttc gagagtgacc tccgccccca
     4381 ccagagccgc tgtgatgatc actggttcca
//
LOCUS       DASRMJ010000097         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_444492, whole genome shotgun sequence.
ACCESSION   DASRMJ010000097 DASRMJ010000000
VERSION     DASRMJ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444492"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFD74_02880"
     CDS             complement(<1..835)
                     /locus_tag="VFD74_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HZK48528.1"
                     /translation="MTQQKPILTAYTVRVSPRARRASLRMTVDRGLEVVVPPGFPLAR
                     VPGLVADKAAWIERVTRRFEVERAAAEAAASAGDGADGADGLPRCIELEALAERWKVE
                     YRSTPSTQVRVGEWVRPAAAESGWASGDGSEWARPTSFSPAAGSANDGIDGALTVSGA
                     VGDHHACRAALVRWLSRKAAGHLVSLVADVAREEGLAYSGVTVRGQRTRWGSCSRRGA
                     ISLNRHLLFLPPPLVCYVVLHELCHTVKLDHSARFWEEVRRREPEADRLRRDLRLAGR
                     WV"
     gene            1087..1353
                     /locus_tag="VFD74_02885"
     CDS             1087..1353
                     /locus_tag="VFD74_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZK48529.1"
                     /translation="MPTVRVGRRGQVTIPSEVRRRLELGEGDRLLLSVSQEEIVLRPL
                     SSSILDLRGSIPVSGPQDFTQVRASAISSVAENLVRGTETNGRG"
     gene            1346..1774
                     /locus_tag="VFD74_02890"
     CDS             1346..1774
                     /locus_tag="VFD74_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZK48530.1"
                     /translation="MGELRGALFVDANVFLRYLTNDVPEQAEAVESLFRDAADGHACL
                     VTNVMVLAELVRVMESQYRLSKQEVHRRALAVATMEGLDLPGLDLVVQALFDYLEKNV
                     DFIDAYNVAWAGHRAIDTIVTFDSRHLGRFPGLTVVTPGT"
     gene            1899..2240
                     /locus_tag="VFD74_02895"
     CDS             1899..2240
                     /locus_tag="VFD74_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HZK48531.1"
                     /translation="MKIIDHSIPMADLEAMAREGFGDLVKAVVDVERGIMAIDAELHA
                     DEEALLLENGSTQSDLWGINIYPELEGPDRVEFDSMINIRPSQGNRSRSVDDPDIRRR
                     ILRIVEALLRP"
     gene            2237..2584
                     /locus_tag="VFD74_02900"
     CDS             2237..2584
                     /locus_tag="VFD74_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48532.1"
                     /translation="MSAHHRELAAGRWQEFSLVEQLANVGSEVERALSWTAKRNAAFS
                     AKAFDRALELLDLTIRDPTHRHRLKELTRLREVLLDYFLGENEFSSTPSSWHRYFHPF
                     GVAAALRRHAADH"
     gene            2650..>3369
                     /locus_tag="VFD74_02905"
     CDS             2650..>3369
                     /locus_tag="VFD74_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZK48533.1"
                     /translation="MTFSPHAAALLARIQDRSAVVGVIGQGYVGLPLALVFVEAGFRA
                     FGFDVDPEKITRLERGDSYIRHIGAERVAAARAGLHPEGGARFTPTADFDRLAECDAI
                     LICVPTPLGRHREPDLSYVLDTTREIAARLHPGQLVVLESTTYPGTTDEEVQPLLEAA
                     GLRTGTGARATGDAGAAGPANTDTDALSPGFGAPGVRGPGAGSGVARNSGGGGGVAGV
                     DSRNDFFLAFSPEREDPGNPHF"
BASE COUNT          585 a         1224 c         1046 g          514 t
ORIGIN      
        1 gcacccacct acccgcgagc cgcaagtctc gccgcagccg gtcggcctcg ggctcgcggc
       61 gccgcacctc ttcccagaaa cgagctgagt gatccagctt caccgtgtgg cagagttcgt
      121 gcagaacgac gtagcacacc aatggcggcg gcaggaacag caggtggcgg ttcaggctga
      181 tggcgccgcg ccgcgagcag ctaccccagc gagtgcgctg gccgcgcacc gtcacgccgg
      241 agtaggcaag gccttcctcc cgggccacgt cggcgaccag cgacacaagg tgaccggcgg
      301 ccttccgcga gagccagcgg accagcgccg cccggcaggc gtgatggtca ccgacggcgc
      361 cggacacagt aagcgctccg tcgatgccat cgttcgcgga tccggcggcg gggctgaaag
      421 aagtcggacg cgcccactcg ctcccgtcgc cactcgccca tccgctctcg gcagccgcag
      481 gccgaaccca ctcgccaacc cgaacctggg tagacggcgt cgatctgtac tcgaccttcc
      541 accgctcagc gagggcctcc agttcgatgc accggggaag accatccgcc ccatccgccc
      601 catcgcccgc cgaggccgca gcctcggcgg cagcgcgctc gacctcgaaa cggcgagtga
      661 cgcgctcgat ccaagccgcc ttgtctgcca ccagacccgg cacgcgcgca agggggaacc
      721 cgggtgggac caccacttcc agacccctat ccaccgtcat acgcagcgac gcccgccggg
      781 cacgcgggct cacgcggacc gtgtaggctg tcagaatcgg cttttgctgc gtcatgcgcg
      841 gaacgtcgtc gcgtccatgg gctctcactt cctccagcga tgggccgcgg tcacccgtgt
      901 cctgacggcc accgatgtgc cgaggatcag tatgtcagca atcccaacgc ccaactcagg
      961 cggttcgtcc tcaatgtctc gttcttgcgg acatctctgc tatcatcgga cgatagtaca
     1021 gactactgac gcagcgtgtc accgccctcc aaacgtgcga acctcgagaa caagtgaggc
     1081 gatcacatgc cgacagtgcg agtgggccgc cgcggacaag tcaccatacc gagtgaggtg
     1141 cgccgcaggc ttgagttggg cgaaggcgac cgcctcctcc tcagcgtcag ccaagaggag
     1201 atagttctcc gaccgctgag ttcgtccata ctcgatttgc gggggagcat ccccgtttcc
     1261 ggcccgcagg atttcaccca agtcagggcc tcggcgatca gtagcgtggc ggagaacctc
     1321 gtccggggaa cggagaccaa cggccgtggg tgagttgcgt ggtgctctgt tcgtggacgc
     1381 caacgtgttc ctccgctacc tcacgaacga cgtacccgag caggccgaag cggtcgaatc
     1441 gctgttcaga gatgcggcgg atggccacgc ttgccttgtc acgaacgtca tggtgttggc
     1501 cgagttggtg cgggtcatgg aatcccagta ccggctgtcc aagcaggaag tgcaccgccg
     1561 ggccctcgcg gttgcgacca tggagggact tgacttgccc gggctcgacc ttgtggtgca
     1621 ggctctcttc gactacctcg agaagaacgt ggacttcata gatgcctaca acgtcgcctg
     1681 ggccgggcac cgcgccatcg acacgatcgt caccttcgac tcacgacacc tgggccgctt
     1741 ccccggtctg accgtggtca ctcccggcac gtagggcccg gagcacagca cccgcgaacc
     1801 tagggacggc gtcgccgcgc ccttgccgcc gcgggcaacc gccccagcag gcgctccatg
     1861 gagtgcgcca ccccacctcc ccctgctata gtcgatgcgt gaagatcatc gaccactcca
     1921 tcccaatggc agatctggag gccatggccc gtgaagggtt cggcgacctc gtgaaggccg
     1981 tcgtcgacgt ggaacgtggc atcatggcca ttgacgcgga actccacgcc gacgaagagg
     2041 ctctgctgct cgaaaatggg tccacccaga gtgacctctg gggcatcaac atctaccctg
     2101 aactggaggg ccccgaccgg gtcgagttcg actccatgat caacatccgc ccgtctcagg
     2161 gcaaccgctc gcgttcggtc gacgatccgg acatccgccg gcggatcctc cgaatcgtcg
     2221 aggcactcct cagaccatga gcgcgcacca ccgggagttg gctgccggca gatggcagga
     2281 gttctcgctg gtggagcagc tggccaacgt ggggagcgag gtcgagcggg cgctcagctg
     2341 gacggcgaag agaaacgccg ccttcagtgc caaggccttc gatcgcgcgc tggaactcct
     2401 cgatctcacc atccgtgacc ccacgcatcg gcaccgcctc aaggaactca cccgcctgcg
     2461 ggaagtcctc ctcgactact tcttggggga gaatgagttc agctcgaccc catccagctg
     2521 gcaccggtac ttccatccgt tcggcgtcgc cgcggccctc cggagacacg ccgccgacca
     2581 ctgacgcctc cctccaccgc ccctgataca atggcgaccg tgatggacga ccctcgacaa
     2641 tcgagaccga tgaccttctc cccccatgcc gccgccctgc tggcccgcat ccaggaccgc
     2701 agtgccgtag tcggcgtgat cggccagggc tacgtggggt tgccgctggc gctggtcttc
     2761 gtggaagcgg ggttccgcgc cttcgggttc gacgtcgatc cggagaagat cacccgcctg
     2821 gaacgcggcg acagctacat ccgccacatc ggagccgaac gggtggccgc ggcccgcgcc
     2881 ggcctgcacc cggaaggggg cgcccgcttc acccccaccg ccgacttcga tcgcctggcc
     2941 gagtgcgacg ccatcctcat ctgcgtgccc acgcctctgg ggcggcaccg cgaacccgac
     3001 ctctcctacg tcctcgacac cacccgcgag atcgccgcgc gcctgcaccc gggccagctg
     3061 gtggtgctgg agtccaccac ataccccggc accaccgacg aggaggtgca gcccctgctg
     3121 gaggccgccg gtctgcgaac gggcacaggc gcgagggcga ccggagacgc tggagccgcc
     3181 gggcccgcca acactgacac cgatgccctc agtcccggct tcggcgcccc cggcgtccgc
     3241 ggcccaggcg cgggctctgg cgtcgcccgc aacagcggcg ggggcggggg cgtcgccggc
     3301 gtcgactccc gcaacgactt cttcctggcc ttcagccccg agcgtgaaga cccaggaaac
     3361 ccccacttc
//
LOCUS       DASRMJ010000098         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_187594, whole genome shotgun sequence.
ACCESSION   DASRMJ010000098 DASRMJ010000000
VERSION     DASRMJ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187594"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VFD74_02910"
     CDS             <1..144
                     /locus_tag="VFD74_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZK48534.1"
                     /translation="LLKEAGEAAACCALPIDDIRGTAEYRVDLVRVLTARALRKAITE
                     GHA"
     gene            197..697
                     /locus_tag="VFD74_02915"
     CDS             197..697
                     /locus_tag="VFD74_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZK48535.1"
                     /translation="MASITDKHGIQRYIIDLTVNGDPHSVVVKANTLLVNVLRDQLDL
                     TGTKKGCETGDCGSCTVLLDGKPVDSCLVLALEADGCEIITIEGVAQHGELDRLQKTF
                     VDKAAVQCGYCSPGMILSAKALLTRNPHPTEHEVREAIAGNLCRCTGYVNIVKAVLSA
                     AEEEAR"
     gene            694..>2247
                     /locus_tag="VFD74_02920"
     CDS             694..>2247
                     /locus_tag="VFD74_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZK48536.1"
                     /translation="MSGTEARDYAVIGKSVPKIDGRDKVIGAARYTADLKFPNMLYGK
                     ILTSPYAHARILSIDTSEAEKIPGVNAVVTHKDAPTLKYGISPARWDENIFCIDTVKF
                     VGDKVAAVACIDEATCYKALAAIKVEYEELPAVLDPLHAMDEDQPQIHPEYERNINTE
                     IHQDFGDIEEAFRKAHYVRTDTFVGNRTYQTPIEPHTATSMWDGNKLTVYSSTQSPHY
                     FQYYIAREFGLPMGDVRVLKTYVGGGFGGKLEPTGLEFGGAVLAKITGRPVKMFYDRA
                     EMFAHNRGRHRQYMTLTTGVDEDGKILGVHADFIMDGGAYTSLGIASAYYAGAMLTLT
                     YEFDNFRFDMFRVYTNLPACGAQRGHGAPQPRYAFEAHLDNIAKHLGIDPMDIRILNA
                     RRPNTVTPNEYIVNSCEMKACLEKARVISGWDEKKGKLPKGRGIGVATGSFVTGAGYP
                     IYRTDLPHAAAMIKVGEDGTSATLYTGAVDIGQGSDTILCQMAAEAMGYTYEQMKIVA
                     ADTETTPHDF"
BASE COUNT          465 a          781 c          681 g          320 t
ORIGIN      
        1 ctcctcaaag aggccgggga ggcggcggcc tgctgcgccc tgccgatcga cgatatccgc
       61 gggacggcgg agtaccgggt ggatctggtg cgcgtgctca ccgcccgggc cctgcggaag
      121 gccatcaccg agggccacgc atgacccgcc cgcccgcact cgacgccgcg aaccacagca
      181 aaggggagca ccgctgatgg catccatcac cgacaagcac gggatacagc gctacatcat
      241 cgacctcacg gtcaacggtg atccgcactc ggtggtcgta aaggcgaaca cccttctcgt
      301 gaacgtgctg cgtgatcagc tggacctcac cggcaccaag aagggctgcg aaaccggaga
      361 ttgcggctcg tgcacggtcc tgctcgacgg caagccggtg gactcgtgcc tcgtcctggc
      421 tttggaggcc gacggctgcg agatcatcac catcgaaggc gtggcccagc acggcgagct
      481 cgaccgactc cagaagacgt tcgtggacaa ggccgccgtt cagtgcggct attgctcgcc
      541 ggggatgatc ctctccgcca aggccttgct caccaggaac ccccacccca ccgagcatga
      601 ggtgcgggaa gccatcgccg gcaacctgtg ccggtgcacg ggatacgtca atatcgtgaa
      661 ggccgtactc tcggccgccg aggaggaggc tcgatgagcg gcacggaagc gcgagactac
      721 gccgtcatcg gcaagagcgt cccgaagata gacggccgcg acaaggtcat cggcgcggcc
      781 cgctacacgg ccgacctcaa gttccccaac atgctctacg ggaagatcct caccagccca
      841 tacgcgcacg cccgcatcct ctccatcgac acttccgagg cggagaagat ccccggcgtc
      901 aacgcggtgg tcacccataa ggacgccccc acgctgaagt acggcataag cccggcgcgc
      961 tgggacgaga acatcttctg catcgacacg gtcaagttcg tcggcgataa ggtggcggcc
     1021 gtcgcctgca tcgacgaggc gacctgctac aaagcgctgg ccgcgatcaa ggtggagtac
     1081 gaggagctgc cggcggtcct cgaccccctg cacgccatgg atgaggacca acctcagatc
     1141 cacccggagt acgaacgcaa catcaacacc gagatccacc aggacttcgg cgacatcgag
     1201 gaggccttcc gcaaggccca ctacgtgcgc accgacacct tcgtcggcaa ccgcacctac
     1261 cagacgccca tcgagccaca caccgccacc tcgatgtggg acggcaacaa gctcaccgtg
     1321 tactcgagca cccagtcgcc ccactacttc cagtactaca tagcccgcga gttcggcctc
     1381 ccgatgggcg acgtccgcgt gctgaagacc tacgtcggcg gcggcttcgg gggcaagttg
     1441 gagcccaccg gccttgagtt cggcggagcg gtgctggcca agatcacagg gcgaccggtc
     1501 aagatgttct acgaccgcgc cgagatgttc gcccacaacc gcgggcgcca tcgccagtac
     1561 atgacgctca ccaccggggt cgacgaggac ggcaagatcc tgggcgtgca cgccgacttc
     1621 atcatggacg gcggcgcata cacctcgctc ggcatcgcca gcgcctacta cgccggcgcc
     1681 atgctgacgc tcacctatga gttcgacaac ttccgcttcg acatgttcag ggtgtacacc
     1741 aatcttccgg cctgcggggc gcagcgcggc cacggggcgc cgcagccacg ctacgcgttc
     1801 gaggcgcacc tcgacaacat cgccaagcac ctcggcatcg atccgatgga catccgtatc
     1861 ctgaacgcgc gccggcccaa caccgtcacg cccaacgagt acatcgtgaa ctcctgtgag
     1921 atgaaggcct gcctcgagaa ggcacgcgtg atatccggct gggatgagaa gaagggcaag
     1981 ttgcccaaag gccggggaat cggcgtcgcc accggcagct tcgtcaccgg cgcaggctat
     2041 cccatctacc gcaccgacct gccgcacgcc gcggccatga tcaaggtggg cgaagacggc
     2101 acgtccgcca ctctctacac cggagcggtg gacatcggac aaggctcgga cacgatcctc
     2161 tgtcagatgg cggcggaggc gatgggctac acctacgaac agatgaagat cgtggccgcc
     2221 gacaccgaga ccacgccgca cgacttc
//
LOCUS       DASRMJ010000099         5130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_83635, whole genome shotgun sequence.
ACCESSION   DASRMJ010000099 DASRMJ010000000
VERSION     DASRMJ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83635"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VFD74_02925"
     CDS             <1..1022
                     /locus_tag="VFD74_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48537.1"
                     /translation="VPHTDPQVAIDLVMRSTPEIPAWPQLPRRDFRENMFIQFTEGFP
                     GLRIDEDRERAYVLDELPIDELTGFFEALESNDPTRFPISPEYAAALPLLERALSDTR
                     PPFVKGQVTGPVSFGLGLPREDRRALLYDDTMRDAAVHLIVMKARWQLGLLAAWAPWS
                     TPLIMVDEPSLTQMGSAFVSIPEELAFPALERCLLALPCLAGIHVCGGTDWSRLASLP
                     VDVLNFDAADHLEAFLAHGEAVGAFLRDGELLAWGMVPNDDRARGLTAADTGARVIRG
                     AEALVTADTPGARAAGAATLSVDAILEASFVSPACGTGTLPPELAERCFELTAQTSAW
                     LRARF"
     gene            1243..1923
                     /locus_tag="VFD74_02930"
     CDS             1243..1923
                     /locus_tag="VFD74_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZK48538.1"
                     /translation="MSPYRKRRVERLFCVLGRQQAERARATRSAILSDDMPQRTATIL
                     SNLFQPMLTGTYVLLAIALTSATTWTSGVAWGVGLALLTAGVPSADIFRRMRVRTVDD
                     FHIVVRTQRLGPLLVALSCTAAGLALALAMGAPHPLQASLLVALVTGVVLTSITGVWK
                     ISFHAATAVSALVILTWWLGWGALALAPLAPAVAWSRVALGRHSSAQVVAGGAVGATL
                     ALAVLALY"
     gene            1999..2256
                     /locus_tag="VFD74_02935"
     CDS             1999..2256
                     /locus_tag="VFD74_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK48539.1"
                     /translation="MTKAAGNTPLESRRNTPDFTRARCKQCGICVHFCPKGALKLDAM
                     GNPVLTDPALCNGCRLCEYLCPDFGVKVGPGDGDAAGGGSG"
     gene            complement(2253..2825)
                     /locus_tag="VFD74_02940"
     CDS             complement(2253..2825)
                     /locus_tag="VFD74_02940"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZK48540.1"
                     /translation="MARVILQGELKPLGLLNVLQILCNASLTGALRIYEHGLTFTVYL
                     DRGYVVSAAAPEQEPLGIKLLSKKRITPEQLDRALKLQRLAERRGRELPIGRVLVHQK
                     MVTQKDLEECVFDQIIETICLSMELPDPYFSFARLDSIDPTKFRTFINFQFALLEAFR
                     IADEIRETRTFDPAAGASVDASSLRSPAFS"
     gene            complement(3125..4192)
                     /locus_tag="VFD74_02945"
     CDS             complement(3125..4192)
                     /locus_tag="VFD74_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF166 family protein"
                     /protein_id="HZK48541.1"
                     /translation="MRLFFVFSGDESQESDLPPAFLKSFDDAFAQRVIMHLTNDANLC
                     TGCASFCEHCRDQYPIQFAEDIVGLVQLPARLPMYIDEPADYLPEELEPHDVTVAINV
                     HEDLLLSLPALAKRAGSGALIVPQDGTGWLSAGVRRQVQRTCEELGLESAFPKPACSL
                     DARPDQVRVREFQDRYRIGKPKIVIQSRDGVIREARVVQSAPCGNTYYVAYNLAGQEI
                     GPDLNERVVAKYWHSFPCVGSMLTDPELGDSILHKGGFLHYQAVDEALKEEADLKEEA
                     RVRGEDPEQYVLMRRLAQFGEQKAVPNRLAEVRMMELGLVSDALLEPERIGDIAAADT
                     AADTAATCDEDVPVCSAVAAG"
     gene            4305..5090
                     /locus_tag="VFD74_02950"
     CDS             4305..5090
                     /locus_tag="VFD74_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK48542.1"
                     /translation="MEAQNERSLANVLSPEETLDITGALGDPTRYAMYRAIVDAAGEP
                     LTVSEAADRFSLHPNVARMHLQKLVDVGLVVADIRRTKGGGRPARIYTLSKRVANLQF
                     PPRDYQLLAGLALNVVDTLTAETPELLDKVGYELGREEGRRALKRDGLDPGHHEVNAL
                     LDSLTRTAVALGLYPRVDRHADGSVDLEVRNCVFRELSSQHPDLVCRLHTAMLQGLAE
                     EFVATFQLEGKPAISAGAASCRFTLRLSGAGTLDPEPPESTDS"
BASE COUNT          829 a         1756 c         1634 g          911 t
ORIGIN      
        1 ccgtgcccca cacggatccc caagtcgcga tagatctcgt catgagatcc actcccgaga
       61 tccctgcgtg gccgcaactt ccccgccgcg acttccggga gaacatgttc atccaattca
      121 ccgagggctt ccccgggctg cgcattgacg aagaccggga acgggcctac gtcctcgacg
      181 agctgccgat agacgaactg accggcttct tcgaggctct cgagtcgaac gaccccaccc
      241 gcttccccat ctctcccgag tacgccgccg cactcccgct cctcgagcgt gccctctcgg
      301 acaccaggcc tcctttcgtg aaaggacagg tcaccggtcc ggtctcgttc ggcctcggac
      361 tcccgcgcga agaccgccgc gcccttctct acgacgacac gatgcgcgac gccgccgtgc
      421 acctgatcgt gatgaaggcg cggtggcagc tgggtctgct ggctgcctgg gcgccctggt
      481 cgaccccgct gatcatggtc gatgagccca gtctgaccca gatgggctcc gcgttcgtct
      541 ccatcccgga agagttggcc ttccccgcgc tcgagcggtg tctgctcgcc ttgccgtgcc
      601 tggcggggat ccacgtgtgc ggcggcacgg actggagcag gttggcctct ctccccgtcg
      661 acgttctgaa cttcgacgcc gccgatcacc tcgaagcgtt cttggcccac ggcgaagccg
      721 tcggggcctt cctccgcgac ggcgagcttc tcgcctgggg catggtcccc aacgacgacc
      781 gcgcccgcgg cctgaccgcg gcggacacgg gcgctcgcgt cattcgcggc gccgaggctc
      841 tggtcactgc cgataccccc ggggcccgcg ctgccggcgc cgccaccctg agcgtcgatg
      901 ccatcctcga ggcgtcgttc gtgtctccgg cgtgcggcac aggcaccctg ccgcccgagc
      961 tcgccgagcg ctgcttcgag cttaccgcac agacgagtgc ctggttgcgc gcacgattct
     1021 gaggtgtgat tcgtgtcggg tggcgcctgt tccccccgcc gctccagcat acccccttcc
     1081 cctatatgcc cgcaggtgct atcatggcgt ggaccgtccg attcgaaagg aggtcccaca
     1141 tgaagcgctt tatgctcccg cgggtggccc actgtagcga gggctgccga acggggtccc
     1201 gggcacgacg ataaactgct cgcctgaacg gactctccat cggtgagccc gtatcggaag
     1261 aggcgcgtcg agcgcctctt ctgcgtcctg gggcggcagc aggccgagcg ggcgcgggcc
     1321 acgcgctcgg ctatactctc cgacgatatg ccccagcgaa ccgccaccat tctctcgaac
     1381 ctcttccagc cgatgctcac cggcacctac gtgctcctgg ctatcgccct gacctctgcg
     1441 accacgtgga cctcgggcgt ggcgtgggga gtcgggctgg ccctcctcac ggcgggcgtc
     1501 ccctcggccg acatttttcg gcggatgcgg gtgcgcacgg tggacgactt ccacatcgtg
     1561 gtgcggaccc agcgccttgg acctctgttg gtcgcccttt cttgcacggc tgcgggcctg
     1621 gcactcgcct tggccatggg cgcgccgcac ccattgcagg cgagtcttct agtcgccttg
     1681 gtcactggag tcgtgctcac gtcaatcacg ggagtctgga agatcagctt ccacgcggcc
     1741 acggctgtga gcgcccttgt catcctcacc tggtggctgg gttggggagc gctcgccctc
     1801 gcgcccctgg caccggctgt ggcttggtca cgcgtagccc tgggccgtca ttcgtccgcc
     1861 caagtcgtgg cgggcggagc cgtaggggcg acgctcgctc tcgcggtgct cgccctctat
     1921 tgacgaggtc gcggacccgg gggcgccccg gacacccgtc atgcggtgta caatcggcgg
     1981 caaggaggtc ccacaggcat gaccaaggcc gccggcaaca ccccactcga gtcacggcgc
     2041 aacacgccgg atttcacacg cgcgagatgc aagcagtgcg gcatctgcgt gcacttctgc
     2101 ccgaaaggcg cactgaagct ggacgccatg ggcaaccccg tgctcacgga tcccgcgctc
     2161 tgcaacggct gcaggctgtg cgagtatcta tgccctgact tcggggtcaa ggtcggcccc
     2221 ggggacggcg acgcggctgg cggggggtcc ggctaggaga aggccgggga gcgcagcgac
     2281 gaagcatcga cgctcgcgcc cgccgcagga tcgaacgtcc gggtctccct gatctcgtcc
     2341 gcgatgcgga atgcctcgag tagcgcgaat tggaagttga tgaacgtccg gaacttcgtg
     2401 gggtcgatgg agtccaggcg ggcgaagctg aagtacggat caggcaactc catcgagaga
     2461 cagatcgtct cgatgatctg gtcgaagacg cactcctcga ggtccttctg ggtgaccatc
     2521 ttctggtgca cgagaacccg gccgatcggc agttcccgac cgcgtcgctc cgccagccgc
     2581 tgcagtttca gagctcgatc gagttgctcc ggagttatcc gcttcttgct gagcagcttg
     2641 atcccaaggg gctcctgttc gggcgcagcc gccgagacca cgtacccacg gtccaggtac
     2701 acggtgaaag tgagtccatg ctcgtaaatc cgcagggcgc cggtcaacga cgcgttgcag
     2761 aggatctgca gcacgttgag caatccgagg ggtttgagtt ccccttggag gatgacgcgt
     2821 gccatcagct tcaaccaccc cgtgtaggcg cttccacggc ctcccgcttg cgcgagggcc
     2881 cggcccccgg cccgccggcg ccgtctgacg cccagcggtc ggcttcattc cactcgaata
     2941 cttgtatcgc ggttcgggcg gccggacttt aggttccgtt ccggacggag gctcgacgtc
     3001 gccggcgtgg gccgtcgagg agggtgcggg gacgtggctg tgcaaagcga gcgcaggcgc
     3061 gttgggggcc gtcgacggtg ggcgcgcgga tgtgaggcag tcgaggcccc ggaaagcttc
     3121 ctccctaccc ggcagcgacg gccgagcaga ctgggacgtc ctcatcgcag gtcgcggccg
     3181 tatccgcggc tgtatccgcg gccgcgatgt cgccgatgcg ttccggctcg agaagggcgt
     3241 cggagaccag tcccaactcc atcatcctga cctccgccaa ccgattcggc acagccttct
     3301 gctcaccgaa ctgggcgaga cgacgcatca gaacgtactg ctcgggatcc tcgccgcgga
     3361 cccgcgcctc ttctttgagg tccgcctcct ccttcagcgc ctcgtccacg gcctggtagt
     3421 gaaggaaccc gcccttgtgg aggatgctgt ccccgagctc cgggtcggtc aacatcgaac
     3481 ccacgcaggg gaagctgtgc caatacttag cgaccacccg ttcgttgagg tcggggccta
     3541 tctcctgtcc ggccaggttg taggccacgt agtacgtgtt gccgcacgga gcgctctgca
     3601 cgacccgcgc ctcgcggatc acgccatccc ggctctggat gactatcttg ggcttcccga
     3661 tcctgtatct atcctgaaac tcgcgcacgc gcacctggtc tgggcgcgcg tcgagcgagc
     3721 aggccggttt cgggaaagcc gactcgaggc ccaactcttc gcatgtgcgc tgcacctgtc
     3781 gccgcacccc ggcgctgagc caaccggtgc cgtcttgggg aacgatcagg gccccgctgc
     3841 ccgcccgctt cgcaagggca ggcagactca ggagcagatc ctcatggacg ttgatagcga
     3901 cggtgacgtc gtgcggttcg agctcctccg gtagatagtc ggcaggctcg tctatgtaca
     3961 tcggcagccg agcggggagc tgcacgagac ccacgatatc ctcggcgaac tggatggggt
     4021 actggtcgcg acaatgttcg cagaagctcg cgcacccggt gcacaggttg gcgtcgttgg
     4081 tcagatgcat gatgacgcgc tgcgcgaacg cgtcgtcgaa cgacttgagg aaggcaggcg
     4141 gaaggtcgga ctcctgggat tcgtcgccgg agaacacgaa gaacagcctc acgacaccct
     4201 cctcccgcac gcgttgccgt ttacactgcc gcggtagtat aaaccactgt tgctactgtg
     4261 tcaacggtgt ttaaaacacc ggctcctttc gctactatag tcacatggaa gctcagaatg
     4321 agagatctct cgcaaacgtc ctctccccgg aagagacgct cgacataacc ggggcgctcg
     4381 gcgatccgac ccgctatgcc atgtataggg cgatcgtcga tgccgccgga gagccactca
     4441 cggtgtcgga ggcggccgat cggttctcgt tgcaccccaa cgtggcgcgc atgcaccttc
     4501 agaagttggt agacgtaggc ctggtggtcg ccgacatccg ccgaacgaag ggcggcggca
     4561 ggccggcccg catctacacg ctgagcaagc gcgtggccaa cctgcagttc ccgccgcgcg
     4621 actaccaact gctggccggc ttggctctga atgtggtcga cacgctgacg gccgagacgc
     4681 cggaactcct cgacaaggtc gggtacgagt tggggcggga ggagggccgt cgtgccctca
     4741 agcgtgacgg cctcgacccg ggccatcacg aggtgaacgc cctcctcgac agtctcacgc
     4801 gcaccgccgt cgcactaggg ctctacccgc gcgtcgaccg gcatgcagac ggatcggtgg
     4861 atctcgaggt caggaactgc gtcttcagag aactgtcatc ccagcacccg gacctggtct
     4921 gccgcctgca cacggccatg ctccagggct tggctgagga gttcgtcgcg acattccagc
     4981 tggaaggcaa acccgcgata tctgcgggag ccgcctcgtg ccgcttcacc ctgcgtctct
     5041 cgggcgcagg gaccctcgat ccggagccgc ctgagtctac agattcctga cgaaccggtc
     5101 gatcacgaag cccaggagca gacccagcac
//
LOCUS       DASRMJ010000100         5369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_513322, whole genome shotgun sequence.
ACCESSION   DASRMJ010000100 DASRMJ010000000
VERSION     DASRMJ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513322"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VFD74_02955"
     CDS             complement(<1..1097)
                     /locus_tag="VFD74_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HZK48543.1"
                     /translation="MKRMPLIVGILLIVIVVLVGSGLAYTSSPSFCSSCHTIAPFAEA
                     WVGSSHEQVGVGCIDCHFEPGAVGYIKGKTYSVIKLAQWVIGETERKPEAARTIVAGA
                     CRHCHPDPQATFIPHAFHTEKANLECTECHSGVAHGSELVGVDRAQAAADPKFCNTCH
                     TGDFAPILFTAIEPAGREHPGAPKIDVNVWRNIHWRMADAPAVIDGKPYDKIEKDTCL
                     ACHQEPTVAKACKSCHFARVPEFRLSTSAEQASGLPVGIFAVLFALLMLTVFLKVDDR
                     SRAFSSRWMQGLLVLIGLSDVVVVYFIIRDTLLKETGSLEIGATTVWITYLILSIAIV
                     LLVLYESVIKPGRTRFILLPETDEEEIYVPDP"
     gene            1306..2478
                     /gene="nifS"
                     /locus_tag="VFD74_02960"
     CDS             1306..2478
                     /gene="nifS"
                     /locus_tag="VFD74_02960"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HZK48544.1"
                     /translation="MENIIYMDHAATTPVDPEVLEAMEPWLREQFGNPTTLYSLGLVA
                     HGAMEQARTSIAERLGAKPEEIHFTSGGTESDNWAVKGIADALQNKGKHLITSTIEHH
                     AILEPMEFLEKRGYEVTRVPVDSTGLVDPEEVRGAIRPDTILISIMHANNEVGTIEPI
                     SEIGAIAREAGVTFHTDAVQTVGKIAMDVEALNVDALSLSGHKLYGPKGVGALYIRNR
                     VRVTPLMHGGAQERRRRAGTHNVPGIIGLAKALELATDRMAEESVRERGLRDRLWAGL
                     QERIPDIALNGHPTLRLDNTLNFRVEGIEGEAMILCLDMNLIGVSSGSACTTGSLEPS
                     HVLLAMGIPAEYAHGSLRLTLGHSNSDLDIDYFLDTFPGVVSRLRDMSPVYNKPVA"
     gene            2553..2939
                     /gene="nifU"
                     /locus_tag="VFD74_02965"
     CDS             2553..2939
                     /gene="nifU"
                     /locus_tag="VFD74_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HZK48545.1"
                     /translation="MYSEAVMDHFRNPRNMGVIENADGVGEVGNPTCGDMMRITIKVK
                     EDRLDDVKFQTLGCGAAVATSSMVTEMAIGKTLEEAEDISNKSVAESLGGLPPQKMHC
                     SNLAADGLHEAIKDYRARHSGEPATT"
     gene            complement(2974..4629)
                     /locus_tag="VFD74_02970"
     CDS             complement(2974..4629)
                     /locus_tag="VFD74_02970"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZK48546.1"
                     /translation="MAVTVEQILKLGTLAQARVLSGARGLDHAVTSVTVGEVPDIAEW
                     LSGGEIVMSTMFALTNDTDRQRDFCRRIMAAGAAALFVKPKRFVGAFPLDILEIAEKR
                     GFPIVEVPQEVRWTRIMQEAMEVLIDRQASLLEKSQEIHRQLLEVVISGGGWSDVASA
                     AARLIEKPVLVLDVSLEALGVSADFPWPEAELHRLLDRGQVRSQFESLARSPSKLMHL
                     TEVGVPSLFAVPIVVSHSCLGYVCTITERPVLEEMEKVALEHAATVAAVEMARDQVRF
                     ETEVRLKGDFVDDLIGGRFSSEDSLLRRANFLGCDISHGATVLVADVDEFEKTMTDRR
                     LTEAEVQRLKARLFSRCTRSVSEMEPNSLVSLKSDRVIVFLTGESAVDLKALERLTGR
                     LQSRANEVAELSLSVGVSRFTPELSGLRRAFEEALAALKVGRKLQGAGSVMHFDGVGS
                     YKLLLSIWEHDPDELRGLYAETIDPVDRYDQQNDSQLLKTLTTYLANDENLSKTAEDL
                     YAHRHTIRYRLQKIGDLTGLSVFKSEEKERLSLGLKAKSLLSG"
     gene            complement(4839..>5369)
                     /locus_tag="VFD74_02975"
     CDS             complement(4839..>5369)
                     /locus_tag="VFD74_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZK48547.1"
                     /translation="ALGGLVAITATCAFVDFNAAIIIGAVGGALAVGAALTFDRLRLD
                     DPVGALAVHGIGGAWGTLAVGLFGYTGITSASPVPVGLLVGGGLRQLGVQALGLGATF
                     IWVFAASFALFALIKRTIGLRVSEQEEIEGLDVGEHGAGAYHGDMLGLGRAGGGRSPH
                     PAHEPASVSLALPAEA"
BASE COUNT         1045 a         1746 c         1716 g          862 t
ORIGIN      
        1 gggtcgggaa catagatctc ttcctcatct gtctccggca acaggatgaa ccgcgtgcgt
       61 cccggcttga tcaccgattc gtacagcacg agcaggacga tggcgatgct cagaatcaaa
      121 taggtgatcc agaccgtggt cgcgccgatc tcgagcgatc cggtctcctt cagcagggtg
      181 tcgcggatga tgaagtagac cacgaccacg tccgagagcc cgatcagaac cagcaacccc
      241 tgcatccacc gcgaggagaa cgcgcggctg cggtcgtcca ccttgaggaa cacggtcagc
      301 atgaggagag cgaacagcac ggcgaagatg cccacgggca ggccgctggc ttgttcggcc
      361 gaagtggaga gccggaactc gggcacgcgg gcgaagtgac aactcttgca cgccttggcc
      421 acggtgggct cctggtggca ggccaggcag gtgtccttct cgatcttgtc gtagggcttg
      481 ccgtcgatca cggccggagc gtcggccatg cgccagtgga tgttgcgcca cacgttgacg
      541 tcgatcttgg gggcacccgg atgctcccgc cccgcgggct cgatggccgt gaagaggatg
      601 ggcgcaaagt cgccggtgtg acaggtgttg cagaacttgg ggtcggcagc cgcttgggcg
      661 cggtcgacgc cgactagttc ggagccgtgg gccacgccgc tgtggcactc cgtgcactcg
      721 agattggcct tctcggtgtg gaaggcgtga ggtatgaacg tggcttgagg atcgggatgg
      781 cagtgccggc acgcgcccgc cacgatcgtg cgggccgctt caggcttacg ctcggtctca
      841 ccgatgaccc actgcgccaa cttgatcacg gagtacgtct tgccctttat gtagccaacg
      901 gccccgggtt cgaagtggca atcgatacag ccgacgccca cctgctcgtg gctggaaccg
      961 acccaggctt ccgcaaacgg cgcgatggtg tggcacgaag agcagaacga cgggcttgag
     1021 gtgtaggcaa ggcccgaccc cacaagcacg acgatcacaa tcagaaggat gccgacgatg
     1081 agcggcattc tcttcatccg ggcccccctg gccgtaccgc agcatcgcgg aagcggagtt
     1141 cgatgacggt cagcacggta ccacacctct acccgggtct tcaacagaac gttgggattg
     1201 caccgaggaa atgttggaaa ccctatcagt ggagatgcat ccgcgaaccg aaggcgtcgg
     1261 gtcaggtgct actattggag cggcaatcag cgaggaaggc agtcgatgga gaacatcata
     1321 tacatggacc acgccgccac gaccccggtg gacccggaag tcctggaggc catggagcct
     1381 tggctccgcg agcagttcgg caaccccacg acactctact ccctgggcct ggtcgcgcac
     1441 ggcgccatgg aacaggcacg cacatcgatc gcggagcggc taggagccaa gccggaggag
     1501 atccacttca ccagcggagg caccgagtcc gacaactggg ccgtcaaggg catcgccgac
     1561 gccctgcaga acaagggcaa gcacctcatc accagcacca ttgagcacca cgcgatcctg
     1621 gagccgatgg agttcctcga gaagcgcggt tacgaggtga cgagagtccc tgtcgactcc
     1681 acaggtctgg tcgatcccga agaggtgcgc ggggcgatcc ggcccgacac gatccttatc
     1741 tcgatcatgc acgccaacaa cgaagtgggc accatcgagc ccataagcga gatcggcgcc
     1801 atcgcccggg aagcgggcgt caccttccac accgatgcgg tgcagacggt gggcaagatc
     1861 gcgatggacg tcgaggccct caacgtcgac gcgctctcgc tctcaggaca caagctgtac
     1921 ggccccaagg gggtcggggc cctctacata cggaaccgcg tgcgggtgac ccccctcatg
     1981 cacggcggcg cccaggagcg ccgccggcgc gccggcacgc acaacgtgcc gggcatcatc
     2041 ggcttggcca aggctctgga gttggccacg gacaggatgg ccgaggagtc agtgcgggag
     2101 cgcggcctcc gcgaccgctt gtgggccggc ctgcaggagc ggatacctga tatcgcgctc
     2161 aacgggcacc ccacgcttcg tctcgacaac accctgaact tccgagtcga aggcatcgag
     2221 ggcgaggcca tgatcttgtg cctcgacatg aacctgatag gcgtctcctc ggggtcggcc
     2281 tgcaccaccg ggtcgctcga accgagccac gtgctgctgg cgatgggcat cccggcggag
     2341 tacgctcacg gctctctgcg cctcacgctg ggacattcga attccgatct cgatatcgac
     2401 tacttcctgg acacgttccc gggggtcgtc tcacggctgc gggacatgtc tcccgtgtac
     2461 aacaagcctg tggcgtagcg aacgctcggt ccgggaacaa tctccggcag gaacattgaa
     2521 atcacccgcc ttcgggcggt aaggaggaaa cagtgtactc agaagcggtg atggaccact
     2581 tccgcaaccc ccgcaacatg ggcgtgatag agaacgccga tggggtaggc gaagtcggca
     2641 atccgacgtg cggcgacatg atgcgcatca ccatcaaggt gaaggaagac cgtctggacg
     2701 acgtgaagtt ccagaccctc gggtgtggag ccgccgtagc cacctccagc atggtcaccg
     2761 agatggccat cggcaagacg cttgaggaag cagaagacat cagcaacaag agcgtggcgg
     2821 aatccttggg tgggctcccc ccccagaaga tgcactgctc caacctggcg gcagacggtc
     2881 tgcacgaagc gatcaaggac taccgggcgc gccactccgg ggaaccggcc acaacgtagc
     2941 cggggtacgg agcgccaccg cgcactcggc cggttagccg ctgagaaggc tctttgcctt
     3001 caatcccagg ctgagccgct ccttctcctc gctcttgaac accgagagcc cggtgagatc
     3061 cccgatcttc tggaggcgat accggatggt gtgtcgatga gcgtagaggt cttcggccgt
     3121 ctttgagaga ttctcgtcgt tcgccaggta ggtggtgagg gtcttgagga gttgcgagtc
     3181 gttctgctgg tcgtagcggt ccacgggatc gatggtctca gcgtagagcc cgcgaagctc
     3241 gtcggggtcg tgctcccaga tgctcagcaa gagcttgtac gaccccacgc cgtcgaaatg
     3301 catcacgctc cccgcaccct gcaacttgcg gcccaccttc agcgcggcca gcgcctcttc
     3361 gaaggctctg cgtagcccgg acagttcggg cgtgaaccgg ctcaccccca cggacaggga
     3421 gagctcggcc acctcgttcg cacggctctg aaggcgtccg gtcagccgct cgagggcctt
     3481 cagatcgacc gcggactcac ctgtgagaaa gacgatgacc cggtcggact tcaggctgac
     3541 cagcgagttg ggctccatct cgctcacaga gcgggtgcag cggctgaaca ggcgggcctt
     3601 gagccgctgg acctcggcct ccgtgagccg ccggtcggtc atcgtcttct cgaactcgtc
     3661 cacatcggcc acgaggacgg tggcaccgtg gctgatgtcg caccccagga agttggcgcg
     3721 gcgcagcagg gagtcctcgc tgctgaaacg tccaccgatg aggtcatcga cgaagtcgcc
     3781 cttgaggcgc acctcggtct cgaagcgcac ctggtcacga gccatctcga ccgccgctac
     3841 ggtggccgcg tgctcgagcg ccaccttctc catctcttcc agcaccggcc gctcggtgat
     3901 ggtgcagacg tagccgaggc agctatggct taccacgatg ggcacggcga agagcgacgg
     3961 cacgcccacc tccgtcagat gcatcagctt gctcggagac cgggccaacg actcgaactg
     4021 actccgcacc tgcccgcggt cgagcagcct gtggagttcg gcctccggcc acgggaagtc
     4081 ggcggacacg ccgagcgcct cgagggatac gtccaacacc aacacgggct tctcgatgag
     4141 acgagccgcc gcgctcgcga cgtcgctcca tcccccccca ctgatgacga cctcgaggag
     4201 ttgccggtgg atctcctgag acttctccag cagcgaggcc tggcgatcta tcaagacctc
     4261 catcgcctcc tgcatgatcc gcgtccagcg gacttcctgc ggcacctcga ctatcgggaa
     4321 gcctcgcttc tcggcgatct cgaggatatc caggggaaaa gctcccacga agcgcttcgg
     4381 cttcacgaac agagccgctg cacctgcggc catgatgcgg cggcagaaat cgcgctgccg
     4441 atcggtgtcg ttggtgagag cgaacatcgt ggacatgacg atctcgccac cggagagcca
     4501 ctccgcgata tccggcactt ctcccaccgt caccgacgtg accgcgtggt cgagtccccg
     4561 ggctcccgag agcacccgag cttgcgccaa ggtcccgagc ttcaggatct gctccacggt
     4621 cacggccatg tcggactccc cctcacgccg gattcgtcac accggatgcg tcacgccgca
     4681 ttataccggc agatatccgc gtgtacttgt ccaagtgtct aagcgggttg gcaccgactc
     4741 aggcccggga cgcagagagg atggccattc ccgtgtgcaa tgaggatcga acgcccgctc
     4801 cccttcgaga gggagcgggc gttcgatccg gcgcagggct acgcttcagc cgggagcgcg
     4861 agggagacag aggcaggttc gtgtgctgga tgagggctgc ggccacctcc ggcgcggccc
     4921 aggcccagca tgtcaccgtg gtacgctccc gcgccgtgct cgcccacgtc cagtccttcg
     4981 atctcctctt gttcggagac tcgaagcccg atggtgcgct tgatgagagc aaagagagcg
     5041 aaggaggcgg cgaacaccca gatgaaggtg gcgcccagac ccagcgcctg cacgccgagt
     5101 tgcctgagac cgccgcccac cagaaggcca acgggcacgg gggaggccga cgtgatacca
     5161 gtgtagccga acaagccgac ggccaaggtg ccccatgccc caccgatacc gtgcacggcc
     5221 aaggccccca ccggatcgtc aaggcggagc cgatcgaagg tgagcgcagc gcccacagcc
     5281 aaggctccgc ccacggcccc gatgatgatg gccgcgttga agtcgacgaa ggcgcaggtg
     5341 gccgtgatag ccacgagtcc ccccaaggc
//
LOCUS       DASRMJ010000101         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_120833, whole genome shotgun sequence.
ACCESSION   DASRMJ010000101 DASRMJ010000000
VERSION     DASRMJ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120833"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFD74_02980"
     CDS             <1..400
                     /locus_tag="VFD74_02980"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZK48548.1"
                     /translation="VGVRNVFQATVLEAGADGVVLDWDGLVVEAAAQHARVGERVPVY
                     IRPEDVKVMYPDRSVAEALSANRFEGVVVDHHPTGDSTVLRADLPNGHEIEVRYPGYT
                     YTSLTLETGRPVSICLRRDALVLLPARDPH"
     gene            608..2443
                     /locus_tag="VFD74_02985"
     CDS             608..2443
                     /locus_tag="VFD74_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZK48549.1"
                     /translation="MKKLLSGNEAIGYGAYAAGVGYAVGYPGTPSTEILQSYSTYPGV
                     HAEWAPNEKVALDVATGISYAGERTFCTMKHVGLNVAADSLFSIAFTGVRGGLVVAVA
                     DDPGMHSSQNEQDTRNYGKFARLPVVEPGDSAEGAEFMGAALELSEQFKMPVILRSVT
                     RLSHSRSPLEVLDELKPIHPGHETIKYERNPPQQIPLPMNARRMHREVEQKLIDVAAW
                     AETCGLNRIEPGSSKLGIVASGIAYQYAREVFPDATFLKLGLVHPLPRDLFFKLDGMV
                     ERIVVVEELDPIFEHHIRLLGIKASGKDIFPLCGEFSTELVRERAVAAGLIQAEEIAV
                     RSAAPVVINGLDAVAGDGSVPEPLDPAALPARPPMLCPGCAHRPIFYVLQRLRALVFG
                     DIGCYTLGAAPPLNATHTCGAMGAGIGVLHGVDMVGVKDRTVAVIGDSTFFHSGLSPL
                     VNIAYNKGVSTVIVLDNRITAMTGHQPNPGTGRTMEGVETPEVRIEDVARAFGFAKVD
                     TLDPYDLEEVRRVLKDHIDSPEPSVVVARYPCVLNTRERYAPPSVDLEACNMCGTCLN
                     IGCAPIINRGDHVEIDHLLCSGCDYCVSVCPRDAIVSARRDGGEE"
     gene            2443..3057
                     /locus_tag="VFD74_02990"
     CDS             2443..3057
                     /locus_tag="VFD74_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HZK48550.1"
                     /translation="MATGKVEGMTRDKIDFVLAGVGGQGTILASDILVEVGLEAGYDV
                     KKSEVHGMAQRGGGVESQVRWGSKIHSATVEKGRVDYLIGFEMLETARWIEYLKPGGD
                     IVVNRYRIPPPPVNMGVARYPEESEIQELLLTRAGRLEWVDATGIARELGNAALAGVV
                     LLGRLASHLDIEDAVWLGVIERLVPPRFVDLNREAFRRGTESAD"
     gene            3098..3778
                     /locus_tag="VFD74_02995"
     CDS             3098..3778
                     /locus_tag="VFD74_02995"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZK48551.1"
                     /translation="MRGTSNGARRSTEPTKREVIVESALRLILKKGYGSTTVDDVAQL
                     SGVSKGLVSYHYPKKENLFRAVLEKIIAKLEFDLKTTYESSDSARERLRLDFRNLFDS
                     EERTRHYYIVLIDFLAQAPREACVRNYTRVIYQTVLRYVEMTIADGVRTGEFRPVDVK
                     LSASTIVALTEGFVFQWLFNPDGINLEQAYQVSDTYVTQYLLADRTESDGTGPAAPTG
                     KIGIGGRA"
     gene            3775..>4860
                     /locus_tag="VFD74_03000"
     CDS             3775..>4860
                     /locus_tag="VFD74_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZK48552.1"
                     /translation="MTAGTTKSIFSDLTVLSLEQATVLPYLTYRLAQDGVRVIRLEHP
                     KFCDPNRMIGHPFVEGEDRMCSYFFAVNCGKEAVTLNLKDPRAQELLKRTIVELDVDV
                     FATNQLPKNYGQLGVDYATLSAVKPDLIWLGVTGFGPNSNEAAYDPILQARGGLMDLT
                     GEAGGDPQVVGIPLPDMGTSEHAYGQLMKALYKRALTGEGSRIDMAMIDSTTSWLTQP
                     VTMARTFGRPMTRRGNTHEFFGPVSVFPTTDGFVYIALGNDVQWQRLVALPGFEGQAD
                     PAYETNSGRIADMAALNKKLAAATAGFATEDLLATFREATIPIARVQGIDEVCEDPAI
                     APKLLKSVDSVTGFEVTLAPPPIMTEYL"
BASE COUNT          876 a         1587 c         1607 g          790 t
ORIGIN      
        1 ggtgggagtg cgcaacgtgt tccaagccac cgtcctggaa gcgggcgccg acggcgtcgt
       61 gctcgactgg gacgggctcg tggtggaggc ggcggcgcag cacgcccgcg tgggcgagcg
      121 ggtgcccgtg tacatccgtc ccgaggatgt gaaggtcatg tacccggatc gctcggtggc
      181 ggaggctctc agcgcgaacc ggttcgaagg agtggtcgtg gaccaccacc ccacaggaga
      241 ttccacggtg cttcgggccg acctgcccaa cggccacgag atcgaagtgc gctatcccgg
      301 gtacacctac acctccctca cccttgagac cggccggccg gtcagtattt gcctgcgacg
      361 cgacgccttg gtgctgctgc ctgcccggga tccgcactga cccgatcgag atcggggtgc
      421 gtcagcggcg catgtgttgc ccgacgtgag cgggcatgag gcaaccggac catgtcccct
      481 tccgccttga cttgaccgcc gggaatgcct agaatgaaat ccgactgtac gtacagtcag
      541 tcgtgcggac ggcgaccggc cgaccggtcc atccgcacac tgacaggcct ttggaaatgg
      601 ggggcatgtg aagaagctct tatccggcaa tgaagccata ggctacggcg cgtatgccgc
      661 cggcgtagga tatgcggtgg gctacccggg cacgccgagc accgagatac tccagtcgta
      721 ctccacctac ccaggcgtcc acgccgagtg ggcgccgaac gagaaggtgg ccttggacgt
      781 ggccaccggt atctcctacg cgggtgagcg cacgttctgc accatgaagc acgtcgggtt
      841 gaacgtggcg gcagactcgc tcttctcgat cgcgttcacg ggagtgaggg gcggtctggt
      901 cgtggccgtg gccgacgatc ccggcatgca cagctctcag aacgaacaag acacgcgcaa
      961 ctacggcaag tttgccagac tgcctgtggt ggagccgggc gacagtgccg agggcgccga
     1021 attcatgggc gccgcgctcg agctgagcga gcagttcaag atgccggtga tcctccgttc
     1081 ggtgacccgg ctctcgcact cgcgcagtcc cctcgaggtg ctggacgaac tgaagcccat
     1141 ccatcccggg cacgagacca tcaagtatga gcggaatccg cctcaacaga tcccattgcc
     1201 catgaacgcg cgccgcatgc accgcgaagt ggagcagaag ctgatcgatg tcgccgcctg
     1261 ggccgagacc tgcgggttga accgcatcga gcccgggagt tcgaagctgg gcatcgtcgc
     1321 gagcggcatc gcctatcagt acgcccgcga ggtgttcccc gacgccacct tcttgaagtt
     1381 gggcctggtc catccgctgc cccgtgacct cttcttcaag ctggacggca tggtcgagcg
     1441 gatcgtggtg gtggaggaac tcgacccgat cttcgaacac cacatcaggt tgctcgggat
     1501 caaggcgtcg ggcaaggaca tcttcccgct ctgcggggag ttcagcaccg agcttgtgcg
     1561 ggagcgcgcg gtggccgcgg gcttgatcca ggcggaggag atcgcagtcc gttccgccgc
     1621 gcctgtcgtg atcaacggcc tcgacgcggt cgccggcgac ggctcggtgc ccgagccgct
     1681 tgacccggcg gccctgcccg cgcgcccgcc gatgctctgt cccggttgcg cgcaccgccc
     1741 catcttctac gtgctgcagc gcctgcgcgc cctcgtcttc ggagacatcg ggtgttacac
     1801 gctgggggcg gctccgccgt tgaacgccac ccacacctgc ggcgccatgg gcgccggtat
     1861 aggcgtgctc cacggggtgg acatggtggg ggtgaaggat cgcacggtgg ccgtcatcgg
     1921 cgactccacc ttcttccaca gcggactctc tcctctggtg aacatcgcct acaacaaggg
     1981 cgtgagcacg gtgatcgtgc tcgacaatcg catcaccgcc atgaccgggc accagcccaa
     2041 cccgggaacg ggacgcacga tggaaggtgt cgagaccccc gaggtgcgca tcgaagacgt
     2101 ggcgcgcgcc ttcggtttcg ccaaggtgga cacgctcgac ccttacgacc tcgaagaggt
     2161 gcgccgggtg ctcaaggacc acatcgactc gcctgagccg tcggtggtcg tggcccggta
     2221 cccatgcgtg ctcaacaccc gggagaggta cgctcctccg agcgtcgatc tggaagcatg
     2281 caacatgtgc ggcacctgcc tgaacatcgg gtgcgcgccc atcatcaatc ggggcgacca
     2341 cgtcgagatc gaccatctgc tgtgttccgg ctgcgactac tgcgtgagcg tgtgtccgcg
     2401 ggatgccatc gtgtccgcac ggcgtgacgg aggtgaggag tgatggcgac gggcaaggtc
     2461 gagggcatga ccagagacaa gatcgacttc gtattggcgg gagtcggcgg gcagggcacc
     2521 atcctggcca gcgacatcct ggtcgaggtg gggctcgaag cgggctatga cgtcaagaag
     2581 tcggaggtgc acggcatggc tcagcgcgga ggcggcgtcg agagtcaggt gcgctggggc
     2641 agcaagatcc actccgctac cgtggagaag ggtcgcgtcg actacctcat cggtttcgag
     2701 atgctcgaga ccgcccgttg gatcgagtat ctgaagccgg gcggcgacat cgtcgtcaat
     2761 cgctaccgca tcccgccgcc ccccgtgaac atgggcgtgg cgcgctaccc ggaagagtcc
     2821 gagatccagg aactcctgct cacgagggcg gggcgcctgg agtgggtgga cgccaccggc
     2881 atcgcccgcg aactgggcaa cgccgccctc gcgggcgtcg tgctgctggg cagattggcc
     2941 tctcacctgg atatcgagga cgccgtgtgg cttggcgtga tcgagcgctt ggtgccgcca
     3001 cggttcgtgg atctgaaccg agaagcgttc cgcagaggca ccgagtcggc cgactgagcg
     3061 tcggcgggct tcggccatca cggggaaggg atcgaacatg agggggacat cgaacggggc
     3121 gcgtcgctcg acggagccca ccaagcgcga ggtgatcgtc gagtcggcgc tgcgcctgat
     3181 cctgaagaaa gggtacggga gcactaccgt ggacgatgtc gcccaactct ccggggtgag
     3241 caagggcctg gtttcctacc actaccccaa gaaggagaac ctgttcagag cggtgctcga
     3301 gaagatcatc gcgaagctcg agttcgatct gaagaccacc tacgagtcgt cggactcggc
     3361 gagggagcgg cttcgtctgg acttccgcaa cctcttcgac agcgaggagc gcacgcggca
     3421 ctactacata gtgctcatcg acttcctcgc tcaggcgccg cgcgaggcgt gcgtgcgcaa
     3481 ctacacgagg gtgatctacc aaacggtgtt gcgctacgtg gagatgacca tcgccgacgg
     3541 cgtgcgcacc ggcgagttcc ggccggtgga cgtgaagctc tcggcatcga cgatcgtggc
     3601 cctcaccgaa gggttcgtct tccagtggct cttcaatccc gacgggataa acctggagca
     3661 ggcatatcag gtgagcgaca cttacgtcac ccagtacctg ctcgctgacc gcacagaaag
     3721 cgacggcacg gggcccgcgg ctccgaccgg caagatcggc atcggaggac gcgcatgaca
     3781 gccggtacca ccaagagcat cttctccgac ctcaccgtgc tcagcctgga gcaggccaca
     3841 gtgctcccgt atctcaccta tcggctggcg caggacggag tgcgggtcat caggctcgaa
     3901 caccccaagt tctgcgaccc gaaccgcatg atcggccacc cgttcgtcga gggtgaagac
     3961 cgcatgtgca gctacttctt cgccgtgaat tgcggcaagg aagcggtgac cctcaatctg
     4021 aaggacccga gggcccaaga gttgctgaag cgcacgatcg tggaactcga cgtggacgtc
     4081 ttcgccacca accaactgcc caagaactac gggcagctgg gcgtcgacta cgccacgctg
     4141 agcgccgtga agcccgacct catctggctg ggggtcacgg gcttcgggcc caacagcaac
     4201 gaggccgcct acgaccccat cctccaggcg cggggcggtc tcatggatct caccggcgaa
     4261 gccggcggcg atccccaggt ggtcggcatc cccctgcccg acatgggcac cagcgagcac
     4321 gcctacggcc agttgatgaa ggccctctac aagcgggctc tgaccggtga ggggtcccgc
     4381 atcgacatgg ccatgatcga ctccaccacg tcctggctca cgcagccggt gacgatggcc
     4441 cgcaccttcg gccgacccat gacgcgccgg ggcaacaccc atgagttctt cgggccggtg
     4501 agcgtgttcc ccaccaccga tggcttcgtc tacatcgccc tgggtaacga cgtgcagtgg
     4561 cagcggctcg tggccctgcc cggcttcgag ggccaggccg acccggccta cgagaccaac
     4621 tcgggccgca tcgccgacat ggcggcgctc aacaagaagc tggccgcggc caccgccggc
     4681 ttcgccaccg aggacctgct cgccaccttc cgcgaggcca ccatcccgat agcgcgggtg
     4741 cagggcatcg acgaggtgtg cgaggacccc gccatcgcgc ccaagctgct gaagagcgtg
     4801 gactccgtca ccggcttcga ggtcaccctg gccccgccgc ccatcatgac cgagtatctc
//
LOCUS       DASRMJ010000102         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_151728, whole genome shotgun sequence.
ACCESSION   DASRMJ010000102 DASRMJ010000000
VERSION     DASRMJ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151728"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..>2427
                     /gene="leuS"
                     /locus_tag="VFD74_03005"
     CDS             130..>2427
                     /gene="leuS"
                     /locus_tag="VFD74_03005"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZK48553.1"
                     /translation="MPVAYDPESLETKWQEVWAREKTFAAQNPSAEDVASGKGPGSTY
                     VLEMFPYPSGSAHMGHVKNYTMGDVIARFRRHQGRLVLHPMGYDAFGLNSENVAIQTG
                     EHPAAFTERAISTINRQLRRLGVSIDWSRELATSRPEYYRWTQWLFIQFYNKGLAYKK
                     EAPVNWCPSCQTVLANEQVIEGQCERCDSLVEAKKLRQWFFRITDYAERLLDDFDKLE
                     SWPERVITMQRNWIGRSEGAEVVFVVASDQRRAAAAAAEDPVAGREITVFTTRPDTLF
                     GATFFILAPEHPLVEDLVRGTDREDEVKRYVARAMATSAIERAGTEKEKTGVFTGRHA
                     VNPVNGELIPIWVADYVLMEYGTGAIMAVPGHDERDYAFARTYGLPVVEVIGSPDEFK
                     DDHGQLTAAYAGDGLLVNSGAFDGLAKAEAVRKVTEWLKKRGQADFAVNYKLRDWLLS
                     RQRYWGCPIPIVYCDECGEMPVPEDQLPVLLPDVSDYAPKGKSPLAAAEHWINTTCPK
                     CGGPARRDPDTMDTFVDSSWYYLRYASPWRDDVPFDRAAVDYWLPVDQYIGGVEHAIL
                     HLMYSRFFTKVLYDLGLVGFEEPFNNLFTQGMIYYKGAKMSKSKGNVVNPDEHVAKYG
                     ADSLRSYIMFMGPGESDVEWNDQGIEGIYRFLNRVWRQVTDAIEAGWFAAAGPLPEPG
                     TLSPEARALLVKTNQVIDKVTVDIADRFHFNTALAAIMELNNEISAARGAGGGGGGAS
                     GAGGGGGLGDSAEGRRVLAEAVEMLV"
BASE COUNT          453 a          753 c          824 g          397 t
ORIGIN      
        1 caacggcccg gagccactag aatagggcct tgacccggac ggcgtcgtgc gggcgccatc
       61 ctcgcggcgc ccccgtttag gaatccgggg cggcggacga acccgagtgc gcgaaaggaa
      121 catgactcca tgccggtagc gtatgaccca gaatccctgg agacaaagtg gcaggaagtg
      181 tgggccaggg agaagacctt cgccgctcag aacccctccg ccgaagatgt cgcgtcggga
      241 aagggccccg ggtccaccta cgtgctcgag atgttcccgt atccctcggg cagcgctcac
      301 atggggcacg tcaagaacta cacgatgggc gacgtgatcg ctcggttccg gcgccaccag
      361 ggcaggctgg tcctgcatcc catggggtac gacgcgttcg ggctgaactc cgagaacgtg
      421 gcgatccaga ccggtgagca ccctgccgcg ttcaccgagc gggccatctc caccatcaac
      481 aggcagctgc gccgtctcgg cgtatccata gattggagcc gggagcttgc gacgtcccgt
      541 cccgagtact atcgctggac gcaatggctg ttcatccagt tctacaacaa aggcctggcc
      601 tacaagaagg aagcgcccgt caactggtgt ccttcgtgcc agaccgtgct ggccaacgag
      661 caggtgatcg agggtcagtg tgagcgatgc gactccctcg tggaggcgaa gaagctgcgg
      721 cagtggttct tccgtatcac cgactacgcg gagagactgc tcgatgactt cgacaaactc
      781 gagtcgtggc cggaacgcgt gatcacgatg cagaggaact ggatcgggcg ttccgagggc
      841 gccgaggtgg tgttcgtggt cgcctccgac cagcgccgcg ccgccgcggc tgcggccgag
      901 gatccggtcg ccggcaggga gatcaccgtg ttcacgacgc ggccggacac tctgttcggg
      961 gcgaccttct tcatcctggc cccggagcat ccgctggtgg aagacctagt gcgcggcacc
     1021 gatagggagg acgaggtcaa gcgctatgtc gcccgggcca tggcgacgtc cgccatcgag
     1081 cgcgcgggca ccgagaagga gaagacaggt gtcttcaccg gtcgccacgc cgtcaatccg
     1141 gtgaacgggg agctcattcc catctgggtg gccgactacg ttctcatgga gtacggcaca
     1201 ggtgcgatca tggccgtgcc cggacacgac gagagggact acgcttttgc ccgcacctac
     1261 gggctgcctg tggtcgaggt gatcggtagc ccagacgagt tcaaggacga ccacggacaa
     1321 ctgaccgcgg cgtacgcggg cgacggcctt ctggtgaaca gcggcgcgtt cgacggcctc
     1381 gcgaaagcgg aggcagtgcg caaggtgacc gagtggctca agaagagagg ccaggccgac
     1441 ttcgccgtca actacaagct gcgggactgg ctgctctcac ggcaacggta ctggggatgt
     1501 ccgatcccca tcgtgtattg cgacgagtgt ggcgagatgc ccgtgcctga ggatcagctg
     1561 ccggtcttgc tgccggacgt gagcgactac gcgcccaagg gcaagtcgcc gctcgccgcg
     1621 gcggagcatt ggatcaacac cacctgcccg aagtgcggag gccccgcgcg gcgcgatccc
     1681 gataccatgg acaccttcgt ggactcctcc tggtactacc tgcgctacgc ctcgccgtgg
     1741 cgggacgacg tgcctttcga tcgggcggcg gtggactact ggctccccgt ggaccagtac
     1801 atcgggggcg tggagcacgc catcctgcac ctgatgtact cgcgcttctt caccaaggtg
     1861 ctctacgacc tcggtctcgt cggtttcgag gagccgttca acaacctctt cacccaaggg
     1921 atgatctact acaagggtgc caagatgtcc aagagcaagg gcaacgtggt caaccccgac
     1981 gagcacgtgg ccaagtatgg ggccgactct ctccgcagct acatcatgtt catggggccg
     2041 ggggagagcg acgtggagtg gaacgaccag ggcattgaag gcatctatcg gttcctgaac
     2101 agggtgtggc gtcaagtcac cgacgcgatc gaggccggct ggttcgcggc cgcagggccg
     2161 ctccccgagc cggggaccct cagtcccgaa gctcgcgcgc tattggtcaa gacgaaccag
     2221 gtcatcgaca aggtgacggt ggatatcgcc gatcgcttcc acttcaacac cgcgctggcg
     2281 gcaattatgg agctcaacaa cgagatctcc gccgccagag gtgcgggcgg cggcggtggt
     2341 ggcgccagcg gcgccggtgg cggcggcgga ctgggcgact ccgcggaggg gcgccgggtc
     2401 ttggccgagg cggtcgagat gctggtg
//
LOCUS       DASRMJ010000103         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_446248, whole genome shotgun sequence.
ACCESSION   DASRMJ010000103 DASRMJ010000000
VERSION     DASRMJ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446248"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFD74_03010"
     CDS             complement(<1..708)
                     /locus_tag="VFD74_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZK48554.1"
                     /translation="MRYAETGFNLEIDLSRGSIERVATDPRLTALHLGGQGTAAKILW
                     DRVGPEVDPFSPDNLLIFSSGLMHSTPLPGANRTTVDTFSPQTDRYSHSVFGGFFGPE
                     LKHAGYDKIVVRGKAPDFVYLWIHNDKVELRDAAHLQGKGAQETAALLKAELKQPEAQ
                     VAAVGLAAENRVFMATIEHANSSASRGVGVIMGDKRLKAIVVRGTNDINIASPAELFE
                     KSSRMYQEIYDNPDCGDR"
     gene            948..1997
                     /locus_tag="VFD74_03015"
     CDS             948..1997
                     /locus_tag="VFD74_03015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZK48555.1"
                     /translation="MARRIEAYDLARTFAIIFVFVAHIVIEQTTSTTLRVAFAAVSPG
                     LTMSLLGFISAALLSARAEETGAFLAKRFTRIYIPVWLCLSAVLIMQSFLGTSRFNAD
                     TVVHFMGLSGVYALLSAPNYASVGAGLWFVTVILMMYLLLPLLRRLFGHRNGLVHLLI
                     VIGLSLAANQWLHAGGAWNVVIAFCLGTYLGVNGRLEALNRRSAGWSTVLAASLLVVS
                     ALATADVIPYWFRGLILPLYPLAFIPVFFFAARVLPRPVRAASTFFAALIYEFYILSF
                     YFINDGFRDLFGPSVRMRLQIPIGFVVTLVVAYVLFRVGSRVRRVAETYLLGERGHAA
                     DGRPTPDPGPEGTSS"
     gene            2318..>2502
                     /locus_tag="VFD74_03020"
     CDS             2318..>2502
                     /locus_tag="VFD74_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK48556.1"
                     /translation="MKISKRMLFILVPVLVLVLTGAVAFAAVGGPFPDVPATHWAYSN
                     IMKLAGQGVIVGYQDGT"
BASE COUNT          404 a          789 c          785 g          524 t
ORIGIN      
        1 gcggtcgccg caatccgggt tgtcgtatat ctcctgatac atgcggctcg acttctcgaa
       61 gagctcggcc gggctggcga tgttgatgtc gttcgtgccg cgcacgacga tggccttcaa
      121 cctcttgtcg cccatgatca cgcccacccc gcgcgacgcg ctggagttcg catgttcgat
      181 ggtggccatg aacaccctgt tctcggcggc cagtccgacg gcggcgacct gggcctccgg
      241 ctgcttcagc tccgccttga gcagcgccgc ggtctcttgg gcgcccttcc cctgcaggtg
      301 ggccgcgtcg cgcaactcga ccttgtcgtt gtggatccac aggtagacga agtcgggggc
      361 cttgccgcgg acgacaatct tgtcgtatcc cgcgtgcttc agttcgggtc cgaagaagcc
      421 cccgaacacc gaatgcgagt agcggtcggt ctggggcgag aaggtgtcca cagtggtgcg
      481 gttggcgccc ggaagagggg tggagtgcat cagaccactg ctgaagatca ggagattgtc
      541 gggagagaag ggatcgacct cggggccgac tctatcccag aggatcttgg cggcggtgcc
      601 ctgaccgccg agatggaggg ccgtcagtct gggatcggtg gctaccctct cgatgcttcc
      661 acgagagagg tcgatctcca gattgaaccc tgtctctgcg tacctcatct ggcttcccct
      721 gaatcgttaa ctgaccgacc aaccaactat cgcactatag caccactcgg gcggtgcagc
      781 tacccggatt cgcccgcttc tgtgacggcc gcgagagaga tcccgttagc gatgccggtt
      841 acggtgtcgt cttgtgagga gcagcgccag taaataagct tcgccgtcga acgacggacg
      901 ccgggggagg gacgacggcc gagcctcgcc caggacgcgg agaaccgatg gccagacgaa
      961 tcgaagccta cgacttggcg cgcaccttcg cgatcatctt cgtgttcgta gcgcacatcg
     1021 tcatcgaaca gaccaccagc accaccttga gggtggcttt cgccgccgtg tccccgggtc
     1081 tcaccatgtc gctactcggc ttcatcagcg cagccctgct ctcggccagg gcggaagaga
     1141 ccggcgcgtt cttggccaag cgattcaccc gcatctacat cccggtctgg ctgtgcctct
     1201 ccgcggttct catcatgcag tcgttcttgg gaacgagcag gttcaacgcc gatacagtcg
     1261 tgcacttcat gggactgtcg ggcgtctacg ccctgctctc cgcccccaac tacgccagcg
     1321 tcggggcggg gctctggttc gtcacggtga tcttgatgat gtatctcctg ctcccgctac
     1381 ttcgacggct cttcgggcac cgcaacggcc tggtccacct gttgatcgtc atcggattga
     1441 gcctcgcggc aaaccagtgg ctgcacgccg gcggagcgtg gaacgtagtc atcgccttct
     1501 gcttgggcac gtatctcggg gtcaacgggc gtctcgaggc gctcaaccga agatcggccg
     1561 gctggtccac agtccttgcc gcctccctgc tggtggtatc cgcgctggcc accgccgacg
     1621 tcattcccta ttggttccgc ggccttatcc ttcccctcta cccgctggct ttcatccctg
     1681 tgttcttctt cgcggcgcgc gtactcccgc gccccgtgag ggcggcttcc acgttcttcg
     1741 cggccctcat ttacgagttc tacatcttga gcttctactt catcaacgac ggctttcgcg
     1801 acttgttcgg accgtccgtg cgcatgcgcc tgcagatccc catcggcttc gtggtgaccc
     1861 tggtggtcgc ctatgtcctg ttccgcgtgg gctcgcgcgt gcgccgagtg gcggagacgt
     1921 acctgctggg cgagaggggc cacgccgccg acggccggcc cacgcccgat cctggacctg
     1981 agggcacgtc gtcgtagccg gaggattggg gcggccgggc gagaagaaca ccaggcaagc
     2041 ggtcatccgg ggcgccgcac gcaccccctt cggcccgtct gagggcttca tgtgtgctct
     2101 ttcactattg actcaggcgc caagggacgg taccgtggtg acgtactctt tgaaagtgcg
     2161 ccgtaggcgt gtgggagagt cgcggttggt cgcgccgtta tgtggtgcaa ttcacgtgcg
     2221 tgccgcggga atacggaatt gacggggtgg tgatgccata aggccagaga tcggacttac
     2281 ccagcagtgt tctcaaatag tgtagaggga gaactgaatg aaaatctcaa aaaggatgct
     2341 gttcatcctg gtgcccgtgc tggtgctggt gttgaccggc gccgtcgcgt ttgcggccgt
     2401 gggcggcccg ttcccggatg tcccagcaac tcactgggcg tacagcaaca tcatgaaact
     2461 cgcgggacaa ggtgttatcg tgggctacca ggacggcact tt
//
LOCUS       DASRMJ010000104        13254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_225852, whole genome shotgun sequence.
ACCESSION   DASRMJ010000104 DASRMJ010000000
VERSION     DASRMJ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225852"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFD74_03025"
     CDS             <1..940
                     /locus_tag="VFD74_03025"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HZK48557.1"
                     /translation="GPPGHGRRCDGDAGGDCGDDGDEDTAPPAQGRAPSETGETATQS
                     IHDRVRMKEPGLERMLFVDAYPRAAFMDHFYATDTGVAELVAGTAVELGDFAGSAYTV
                     ASLEEVPHAPAVTLARTGTVAGHSVSIRKTISLDRGHEDTDGVTVRYVIEVAESTAVA
                     DGRDFTDVLFAPEVNLTLLAGWSHDRYVLVDDVPPPACFLADSGTHPGATSVTLVDET
                     RGLRVRLSWGRAAGLESTHEAREVTVAEWEAAIHAVPAEAAATTLLRHGVVTVSLSED
                     GFERIYQSTALLPSTPMRLEAGRRLLATLRLDVSGS"
     gene            937..3363
                     /locus_tag="VFD74_03030"
     CDS             937..3363
                     /locus_tag="VFD74_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HZK48558.1"
                     /translation="MNEPLDVAVVWHMHQPYYGLSDTGAFEMPWVRTHAVKDYAEMVD
                     VLEGYPRLHQTFNLVPSLVEQLLEYATGRYVDTYQEHARRPAAELTPRERAFVLEVMC
                     ERSSHPRARRHPRYLELAQKRDRCTTGKRVRVEEFTDAELRDAQVWFDLAWFHPTHLE
                     EGPLADLVARGGDFTEEDKDAVFAAQAALLSQVLPTYRRAAAAGRIELTTSPYFHPIL
                     PLLANSDLARVSLPEVELPTRRFDHPEDADEHIARALRFHTEVFGKTPRGMWCSEQTV
                     GEDVIAPLSRHGIAWTISDEAVLGRSLGRALQRDAGGRLSDPDFLYSAYRLERERGAI
                     DIVFRDRTLSDLVGFTYRDWDPGDAAADLLRRLRQARHDLDSSDAPHLVTIALDGENA
                     WEYYRNEGRDFLERLYEGLQQDESLRCVTVSEHLDEAPPTRGIPWLHTGSWIGGDLGT
                     WVGDRAHRSAWDLLHDARDAVARVRARTTLPASAEPTDATTPLATATAKPTDAPSRPA
                     PVAAEPSDIDAAWREIMIAEGSDWFWWFSTHQESGLDEVWDAAFRRHLQQAYRFAGLR
                     PPPALFAPILECAPGGAGGSLTPTVDGHLTTPDEWEQGVRLARAGGGAMQRAGGSPVL
                     DLRFGWSNEGLFFLVVPRFPVQETPLDVVFYLGRSGGVAHGDTLRPDRPALLADAAIV
                     ATLGFVPGYRVALTLRGPGSDLSAAASFSAAPFLTAPFLTAPADGEPAPEPGAAPPGD
                     AAVAETVRDAVAETALGDVAVGTVVEVAVPWGLFGALGTDSLLVGAELRDGDKVVDVF
                     PSTGARAKSP"
     gene            3488..4834
                     /gene="trkA"
                     /locus_tag="VFD74_03035"
     CDS             3488..4834
                     /gene="trkA"
                     /locus_tag="VFD74_03035"
                     /inference="COORDINATES: protein motif:HMM:NF007032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HZK48559.1"
                     /translation="MYVVIIGAGEVGFHIASMLTQEGHEVALIDRDPEIIRRASEELD
                     VLTIAGNGASKRVLQQARVSQANIVVAATDSDEVNMIACMAAKHVGVPLTIARVRNPD
                     YLDENASVSTEFTGIDFVIQPEAALAEEIAKIAELPGALDAETFADGQVHMIEVQVDE
                     KSKAVGKPLLKIGIPRNVLLTAVLREDDMTIPTGQTVLRARDRVFLTGKRQGVLEAAA
                     MLSQSMRVSKRAMLLGCGSLGLQIATALETHGFRLTIFEKDYERSVHAAGVLRHSLVI
                     HNEGLDADILEREGVRDMGLFIAATGDERLNMLSSLLAKRLGARRTISIVERTAFSTI
                     LESIGVDLALSPRRITASRILKFMRSGDVLSASILDKSAGEVLEFVVAEDSIVAGRPL
                     RDTHFPEGAIVGALVQADGVHIAHGTSVPQAGDRAIVFALPRAVADVERVFSGDHR"
     gene            4831..6273
                     /locus_tag="VFD74_03040"
     CDS             4831..6273
                     /locus_tag="VFD74_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HZK48560.1"
                     /translation="MNFRFVGHMLALVILAIAVGILASAAVAAGYGEDDLTALLASAG
                     IAVLVGAPLYFSTRLRSRTSIGYREGFLAVGGGWLTAMVFGALPYLFYGMFSVVDALF
                     EAISGFTTTGASVLVDFNQPHGIMFWRSLTHWYGGMGIIVLFIAILPPLGGGAIRLFS
                     AEAPGPVPERLTPHIRDTARGLWYIYVGLSTIEALALLLAGMPLYESVTHTFGTMATG
                     GFSPMATSIAAYDSWPIEFIITVFMVLAGGNFALYFAFLQGNRLKLYRDPEFRLYLGI
                     VGVSVVALTISLVVSGSHFSGLHAFRESLFQVVSIQSTTGYVSADFNQWNSFAKTTLL
                     LLMFIGGSAGSTAGGFKVVRILVLAKNARHDLVRQVHPNAVLPVKVGGRTVPDGLRTA
                     VLGYFFIYMLVFAVGTLLVSASNVSIITAASAVAATLNNIGPGLELVGATQNYAVIEP
                     FAKVVMIGLMVMGRLELMPILLLFTRGFWR"
     gene            complement(6382..7029)
                     /locus_tag="VFD74_03045"
     CDS             complement(6382..7029)
                     /locus_tag="VFD74_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZK48561.1"
                     /translation="MSRQFVVIGLGRFGASVARALTQLGEEVLGIDVDEEKVQALSDE
                     LLHVVVADATDPAVLVSLGLRNFDVAVVSIGRRLQDSILITLALKEAGTPRVVARAVD
                     EMHGRVLRKVGADRVFFPERDMGIRAAHSLVGSDIIDYIEFGSDYSIMEIAAPAKFVG
                     RTLAELGIRAKYGITVLAIKRGSDVLTAPKAADTVEEGAILVMMGRNADLRKITD"
     gene            7269..8195
                     /locus_tag="VFD74_03050"
     CDS             7269..8195
                     /locus_tag="VFD74_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HZK48562.1"
                     /translation="MGAHAYITFLSDYGWAGGYVAACEATIASISPATRVLHICHEIA
                     LGDVRDGGAVLAHVAPLGPPAVHLAVVDPGVGSDRGAVAIHVSRGDYLVGPDNGLLLP
                     AIEALGGVEDAWLLDARTVRTQGRLTPVEPSHTFHGRDVFAPAAALLATGLPPAVLGR
                     AIASEALCPATKPLAEVGPGLARLEVIEIDRFGNVALALGLDRLSLRDSCELALTVEE
                     ETEQEWRSRLVRTFSDLKPGELGILQDSWGRASLALNGASAAELLGARLGGVVRISFE
                     TDCAAAGPGPDAPARAPADTPAHAPLGAERRS"
     gene            8192..9310
                     /gene="rlmN"
                     /locus_tag="VFD74_03055"
     CDS             8192..9310
                     /gene="rlmN"
                     /locus_tag="VFD74_03055"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZK48563.1"
                     /translation="MTAVADTAGDRLTAGDGLTARDRLALYRPDLTQVLSAADAPHYR
                     YAQVYEHLMRRPEAPLSAASNLPLALRTALEEAGSCSLTLEKRREDAEGTTKVLLTAR
                     DGAQIEAVAMRYNRRVTVCLSTQVGCSLGCTFCSTGAMGFARDLTVAEIVDQVRLFIP
                     LLREESREITNIVFMGMGEPLLNLDAVLAAIRLLKDPDGLGFAQRSLSVSTIGIPAGI
                     RRLAREEPQVNLAISLHAPNDELRTRLIPANRRFPLSEVLTAADDHFAVTHRKLFVEY
                     ILLEGVNDTRRHAEALARLLRGRVLTVNLIPWNPGCGDYEPSQPETANAFRDTLQTRG
                     LEATLRLGRGRAIAAGCGQLATKTAARGGAKRRTRRPT"
     gene            complement(9377..9928)
                     /locus_tag="VFD74_03060"
     CDS             complement(9377..9928)
                     /locus_tag="VFD74_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZK48564.1"
                     /translation="MLTLQGFYGQTVEIAGDRSYDAEEELWVQSLAEGVRIGVSKVGV
                     LLMGGIESVEFFVEPGDVVSAGDDLVFFETFKAVRYVGAPLSGTILAVNQKVVDEPEL
                     FDDDPYGVAWLVEMLPAPGQDVDALLVPPEAYLRGLERSEHCAEGSEVLLEAKKGSPT
                     CRSVYGGLRGNDPGEGSLSHRSR"
     gene            complement(9928..10152)
                     /locus_tag="VFD74_03065"
     CDS             complement(9928..10152)
                     /locus_tag="VFD74_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48565.1"
                     /translation="MAAEFGEGAVEVEVVTLKEAAGAERYRELRRLAGEHLPVPCVLV
                     ECRLVSAGIPEQDELRRVVAAALAASGQGD"
     gene            complement(10259..10879)
                     /locus_tag="VFD74_03070"
     CDS             complement(10259..10879)
                     /locus_tag="VFD74_03070"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HZK48566.1"
                     /translation="MAVEKVTVFPCGGIGDPVSSVTRLAGYILADDLLPGRVELVSIP
                     SVITGVPDSLELVQAYPVIALDGCGHRCGSNALNLVGIKPAARLFTPRVRSETKNAIG
                     RRRPFPEVSGQRLAKDLAERAAVIARHMLEDVEYTFERQAISPSPDGLHDESFDIEEA
                     LGYEEMEAGCFHCSGMCAFKLEKDPELRRRLEEEIKEYEELEGFEC"
     gene            complement(10869..11528)
                     /locus_tag="VFD74_03075"
     CDS             complement(10869..11528)
                     /locus_tag="VFD74_03075"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HZK48567.1"
                     /translation="MAVRRICMHACGGITKVGATVARLATYVVAEELVPEKTLISCAP
                     AFLRGVEEDVLMVVKSPSIALDGCEWSCGTNLLFLAGITPAVRFLLPEYAEAQGYDLS
                     GGSRRYPSGVTKDFSRRVAERVAGIAAGMMESGSPYRFERPELKKQPEKWFRSAPNME
                     GVMEFVRIEPGVYRPATMPPLPEESAGTAGDPAPAVQGSARVAGEVGDGGAGEEVTDG
                     G"
     gene            complement(11660..11911)
                     /locus_tag="VFD74_03080"
     CDS             complement(11660..11911)
                     /locus_tag="VFD74_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48568.1"
                     /translation="MPDDVKEFIEYKEWSIKNPEGIGMFMERKGRVLPTLCVNTECVY
                     ESIIPTLDELYETLIGAAYSDEQRDTLKKAYAKAQEEYE"
     gene            complement(12006..12146)
                     /locus_tag="VFD74_03085"
     CDS             complement(12006..12146)
                     /locus_tag="VFD74_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48569.1"
                     /translation="MPFDVPVENVDKVLEVKTWYETYYAHYPDVPYPALQPMRSPAKV
                     GG"
     gene            complement(12150..13082)
                     /locus_tag="VFD74_03090"
     CDS             complement(12150..13082)
                     /locus_tag="VFD74_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK48570.1"
                     /translation="MGKQAVIDAYLGKYTPVVPWVPYVGVHGAFLIGETATTVLQDSD
                     LLAKAVVNAAERYKADGIPLVFDLAVEAMSMGIEAKWFDDNPPTVIVHPLIDKTLAEA
                     NLKVPGPNDGRWPVIIEAGKKAKAQLGDVALIGLVCGPMTLASHLRGAKIFTDMVKKP
                     ELAMEIMEFAALVCAESARIYAEEIGCDIVAIVEPVGSQVRPETYDKFVIPTCQPALD
                     VIHKAGKVSALYICGDATKIVENMMYTGASCVCVDEQLNMNYVRDCALKYGRGFGGNL
                     KLTLALSLGIIDPREDAIVSLAAGGKVGYTFSPG"
BASE COUNT         2194 a         4634 c         4184 g         2242 t
ORIGIN      
        1 gggcccgccc ggccacggcc gccgctgcga cggcgatgca ggcggcgact gcggtgacga
       61 tggcgacgaa gacaccgccc cgcccgccca aggacgtgcg ccgagtgaga cgggcgaaac
      121 ggcaacgcag agcatccacg accgggtgcg gatgaaggag cccggactgg aacgcatgct
      181 gttcgtcgac gcatatcccc gggccgcctt catggaccac ttctatgcca ccgacaccgg
      241 agtggccgag ttggtcgccg ggaccgcggt cgagctcggc gacttcgcgg gttccgccta
      301 caccgtcgcc tcgctggagg aagtcccgca cgcgcccgcc gtgaccctgg cgaggaccgg
      361 tacggtggcc ggacacagcg tatcgatccg caagacgatc tcgctcgaca gaggtcatga
      421 agacaccgat ggcgtcacgg tccgctacgt gatcgaggtc gcggagagca cggccgtggc
      481 cgacggccgt gacttcaccg acgtgctctt cgcgcctgag gtcaacctga ccctgctcgc
      541 cggctggtcg cacgaccgct acgtgctggt ggacgacgtc cccccgccgg cctgcttcct
      601 ggccgacagc ggcactcacc cgggcgccac gtcggtgacc ctcgtcgacg agacccgggg
      661 tctgagagtc cgcttgtcgt ggggccgagc ggctgggctc gagagcacgc acgaggcgcg
      721 ggaggtcacg gtcgcggagt gggaggccgc gatacacgcg gtcccggccg aagccgccgc
      781 caccacattg ctgaggcacg gagtggtcac cgtgtcgctg agcgaggacg gcttcgagcg
      841 catctatcag agcaccgccc tgctgccctc cacgccgatg cgcctcgagg cggggcgcag
      901 actgctcgcg accctgcgac tggacgtgtc aggctcatga acgagccgct cgacgtagcc
      961 gtagtctggc acatgcacca gccctactac gggctctccg ataccggagc gttcgagatg
     1021 ccgtgggtcc gcacgcacgc ggtcaaagac tacgccgaga tggtcgacgt cctcgaggga
     1081 tacccgcgac tccaccagac tttcaacctg gtgccctcgc tggtcgagca gctccttgag
     1141 tacgcgaccg gtcgctatgt ggacacctac caggagcacg cccgcaggcc ggccgcagag
     1201 ctcaccccgc gcgagcgcgc cttcgttctc gaggtcatgt gcgagcgctc gtcgcaccct
     1261 cgcgcccgtc gccacccgcg ctatctcgag ctggcccaga aacgagaccg ctgcacgaca
     1321 ggcaagcgcg tgcgggtgga ggagttcacc gacgccgagc tgcgtgatgc gcaggtgtgg
     1381 ttcgacctcg cgtggttcca ccccacgcac cttgaggagg ggccgctggc ggatctggtc
     1441 gcgcgaggag gcgatttcac cgaagaggac aaggacgcgg tcttcgcggc gcaggccgcc
     1501 cttctatccc aggtgcttcc cacgtaccgt agggctgcgg ccgccgggcg gatcgagctc
     1561 acgacctctc cgtacttcca cccgatcctg cccctgctgg ccaactccga cctggcccga
     1621 gtctcgcttc cggaggtgga gttgcccacg cgtcggttcg accacccgga agacgcggac
     1681 gagcatatcg cgagggccct acggttccac accgaggtct tcggtaagac gccacggggc
     1741 atgtggtgct cggagcagac cgtgggggag gacgtgatcg ctcccctctc caggcacggc
     1801 atcgcctgga cgatctcgga cgaggcggtc ctcggtcgct ctctggggcg cgcgctgcag
     1861 cgcgacgcgg gagggcgcct ttcggacccc gacttcctgt actcggccta caggctggag
     1921 cgggaacgcg gcgcgatcga catcgtgttc agggaccgca ccctctctga cctcgtgggc
     1981 ttcacctata gggactggga ccccggagac gccgccgccg acctgctccg tcgccttcgg
     2041 caggcccgcc atgacctgga ctcgagcgac gcaccgcatc tggtgacgat cgcgctcgac
     2101 ggtgagaacg cctgggagta ctaccgcaac gagggacgcg atttcctcga gcgcctctat
     2161 gaagggctgc agcaggacga gtcactgagg tgcgtcaccg tctccgagca tctcgacgaa
     2221 gccccgccca cgcgcggcat cccctggctg cacacggggt cgtggatcgg aggcgacctg
     2281 ggcacctggg tcggggaccg cgcccacagg tcggcatggg acctgttgca cgacgccagg
     2341 gacgctgtgg ctagagtgcg ggcgcggacg acacttccgg catcggccga gccgacggac
     2401 gcaacaaccc cgctggccac tgcgacggcc aagccgacgg acgcgccgtc gcgaccggcc
     2461 cctgtggcgg ccgagccgtc agacatcgat gcggcctggc gagagataat gatcgccgag
     2521 ggcagcgact ggttctggtg gttcagcacg caccaggaga gcggcctcga cgaggtgtgg
     2581 gacgcggcct tccgccgaca cctgcaacag gcgtaccggt tcgccggcct cagaccgccg
     2641 cctgccctct tcgcccccat cctggagtgc gcaccggggg gtgcgggcgg ttcgctgacg
     2701 ccgacagtgg atggacacct caccaccccc gacgagtggg aacagggagt ccgtctcgct
     2761 cgggcgggcg gcggcgccat gcagcgggcg ggcggttccc cggtcctcga cctgcggttc
     2821 ggctggtcga acgaggggct cttcttcttg gtggtgccac gcttcccggt gcaggagaca
     2881 cccctggacg tggtcttcta cctgggtcgc tcgggcggcg tcgcgcatgg agacactctg
     2941 cgaccagaca gaccggcgct cctcgccgac gccgcaatcg tcgccacgct cggcttcgtg
     3001 cccggctacc gagtagcgct gacgctgcgc gggccgggca gcgatctctc agcagcggcc
     3061 tcgttctcag cggcgccatt cctgacggcg cccttcctga cggcgcccgc cgatggagag
     3121 ccggcgccgg agccgggtgc cgcgcctccc ggagacgccg ccgtcgccga gacggtgcgc
     3181 gacgccgtcg ccgagacagc ccttggagat gtcgccgtcg gaaccgtcgt cgaggtggcg
     3241 gtgccgtggg gcctcttcgg cgctctgggc accgactctc tcctcgtcgg ggcagagttg
     3301 cgcgacggcg acaaggtagt cgatgtgttc ccctccaccg gcgcccgcgc gaagtcaccc
     3361 tgatcagggc gccctcgaac gcgaggctcc tcccaccggg gcgagtatcc cctctgcgcg
     3421 gccgccaacg cggccgtccc cgggcgcaca cctccctcgt gggactgctt gttggtatcc
     3481 tcatgccatg tacgtggtaa tcatcggagc cggcgaagtg ggcttccaca tcgcttccat
     3541 gctcacccag gagggccatg aggtcgccct gatcgatcgc gaccctgaga tcatccggcg
     3601 cgcctccgag gagctcgacg tgctgacgat cgcgggaaac ggggccagca agcgggttct
     3661 gcagcaggca cgggtgtctc aggccaacat cgtcgtcgcc gctacggact ccgatgaggt
     3721 caacatgatc gcctgcatgg ccgccaagca cgtcggcgtg cccctgacca tcgcgcgggt
     3781 gcgcaacccc gactacttgg acgagaacgc ctcggtatcc accgagttca ccgggatcga
     3841 cttcgtcatc cagccggagg cggcactggc ggaggagatc gcgaagatcg ccgaacttcc
     3901 gggggcgctc gacgccgaga ccttcgcaga cggtcaggtc cacatgatcg aggtgcaggt
     3961 ggacgagaag agcaaggctg tcggcaaacc cctgctcaag atcggtatcc cgcggaacgt
     4021 gctgctcaca gcggtgttgc gcgaggacga catgaccatc cccacaggac agaccgtcct
     4081 gagggcacgc gaccgagtct tcctcacagg caagcgccag ggcgtgctcg aggccgcggc
     4141 catgctctcc cagagcatgc gggtatcgaa acgcgccatg ctcctgggtt gcgggtccct
     4201 cggcctacag atcgcgaccg ctctggagac gcacggcttc cgcctcacca tcttcgagaa
     4261 ggactacgag cggtcggtgc atgctgccgg tgtcctccgc cactccttgg tgatccacaa
     4321 cgaaggactc gatgccgaca tcttggagcg cgagggcgtc cgagacatgg gactgttcat
     4381 agcggcgacc ggcgacgaac gcctgaacat gctctcctcc ctgctggcca aacggctcgg
     4441 ggcgcgcagg accatctcta tcgtggaacg aaccgccttt tccacgatcc tcgagtcgat
     4501 cggagtagat ctcgcgctga gcccccgacg catcacggcc tcgcgcatcc tcaagttcat
     4561 gcgctccggc gatgtgctga gcgcctcgat cctcgacaag tccgcggggg aggtgctgga
     4621 gttcgtggtg gccgaggact cgatcgtcgc cggccggccg ctccgcgaca cgcactttcc
     4681 tgaaggagcc atcgtcggtg cgctcgtgca ggccgacgga gtgcacatcg ctcacggaac
     4741 ctcggtgccc caggcgggcg accgagcgat cgtgttcgca ctgccccgag cagtggccga
     4801 tgtcgaacgg gtgttctccg gcgatcacag gtgaacttcc gcttcgtagg ccacatgctg
     4861 gccctagtga tcctggccat cgccgtgggt atccttgcct cggccgccgt cgccgccggc
     4921 tacggggaag acgacctcac cgcgctcctg gcctctgcag ggatcgccgt cctggtaggg
     4981 gcgcccctct acttcagcac cagacttcgc agccgcacga gtatcgggta cagggagggc
     5041 ttcctcgcgg tgggaggagg gtggctcacc gccatggtct tcggggcgct cccctacctc
     5101 ttctacggca tgttctcggt ggtcgacgct ctgttcgagg cgatctccgg cttcaccacc
     5161 accggagcca gcgtactcgt ggatttcaac caaccgcacg gcatcatgtt ctggcgcagc
     5221 ctcacgcact ggtacggagg catgggcatc atcgtattgt tcatcgccat cctgcctcca
     5281 ctcggtggtg gagccatccg gctgttctcg gcggaagctc ccggaccggt gcccgaacgc
     5341 ctcactcccc acatcaggga cacggcccgc ggcctctggt acatctacgt cgggctgagc
     5401 acgatcgaag ccttggccct cctgctggcc ggcatgccgc tctatgagtc ggtcacgcat
     5461 accttcggca ccatggcgac cggaggcttc tcgcccatgg ccacctccat tgccgcatac
     5521 gactcctggc ccatcgagtt catcatcacc gtcttcatgg tgctggccgg cggcaacttc
     5581 gcactctact tcgcctttct ccaagggaac cgactcaagc tctaccgcga tcccgagttc
     5641 cgcctctacc tgggcatcgt cggggtgagc gtggtagccc tcacaatcag cctcgtggtc
     5701 tcgggctcac acttcagcgg ccttcacgcc ttccgggaat cgctcttcca ggtcgtgagc
     5761 atccagtcga ccaccggata cgtctccgcc gacttcaacc agtggaactc gttcgcgaag
     5821 accacccttc tcctgctgat gttcatcgga gggtccgcgg gctctaccgc cggcggattc
     5881 aaggtggtgc gcatcctggt cctggccaag aacgcccgcc acgacctcgt gcgccaggtg
     5941 catccgaacg ccgtgttgcc ggtgaaggtg ggcggtcgaa cggtgccgga cggacttcgc
     6001 acggccgtgt tgggctactt cttcatctac atgctcgtct tcgcggtcgg aacgctcctg
     6061 gtatcggcgt cgaacgtcag catcatcacg gcggcgtcgg cggtggcggc caccctcaac
     6121 aacatcgggc ctgggctcga actggtggga gccacccaga actacgcggt catcgagccc
     6181 tttgcgaagg tcgtgatgat cggcctcatg gtcatgggca ggctggaact catgcccatc
     6241 ctgctcctct tcacgagagg cttctggcga tagcgggtgg ccgaggcgag cgaccgggcc
     6301 aacgatcggc cgaacccggc gggcgagggt aagccgaacc cggcgggcga ggatcggctg
     6361 gagcggggcg gaacctccgg cctagtcggt gatcttcctt aggtcggcgt tccgacccat
     6421 catgacgagg atcgccccct cttccactgt gtcggccgcc ttgggtgcgg tcaggacgtc
     6481 actgccgcgc ttgattgcca gcaccgttat cccgtacttc gccctgatgc cgagttccgc
     6541 caacgtgcgg ccgacgaact tcgcgggcgc ggctatctcc atgatcgagt agtcggagcc
     6601 gaactcgatg tagtcgatga tgtcggaccc caccaggctg tgagcggccc ggatgcccat
     6661 gtcgcgctca gggaagaaga cgcgatcggc gcccactttg cgcagcaccc tgccgtgcat
     6721 ctcgtccacg gcccgcgcga cgacgcgggg cgtacccgct tccttcagcg ccaacgtgat
     6781 gaggatcgaa tcctgcagcc gccgaccgat gctcaccacc gcgacgtcga agttgcggag
     6841 gcccagactc acgagcacgg cggggtcggt ggcgtcggct acgaccacgt ggagcaactc
     6901 gtcgctcagg gcctgcacct tctcctcgtc gacgtcgatg ccgagcacct cttcccccag
     6961 ttgggtcaag gctcgagcca cgctggcgcc gaagcgcccc agaccgatga cgacgaactg
     7021 gcggctcatg ccgaccaccc cggctctgac gtggggggga ggctgcgcgc cgtgaaggtt
     7081 ccgaccgtct cgaccgcgtc gaagaccgat gtgcgtgcca tattcgaacc aacccttccg
     7141 cagccgtgga cagcggcgat ccgcaccatc cgcaaggagt ccctcgctcc acgagggatc
     7201 ggagacgact ccccggaatg gtagccctcc cggaagggaa acaccacccg tggtgttaga
     7261 atggctggat gggagcccac gcctacatca ccttcctcag cgactacggt tgggccggcg
     7321 gctacgtcgc cgcgtgcgag gcgaccatcg cctcgatctc gcccgcgacg agggtgctcc
     7381 atatctgcca cgagatagcg ctcggcgatg tgcgcgacgg tggggccgtg cttgcccacg
     7441 tcgctcctct gggcccgccg gccgtccacc tggcggtggt cgatccggga gtgggaagcg
     7501 atcgcggcgc cgtggcaatc cacgtctcgc gcggagacta cctcgtgggc cctgacaacg
     7561 gccttctgct cccagccatc gaagcgctcg gaggagttga ggacgcgtgg cttctcgatg
     7621 cgcgcacggt gcgcacccag ggccgcctca cgccggtcga accctcccac accttccacg
     7681 gtcgtgacgt cttcgcgccc gccgccgctc ttctggcaac gggactcccc ccggcggtgc
     7741 tcggccgcgc catcgcttcg gaggcactct gcccggcgac gaagcctctc gccgaggtcg
     7801 gaccgggctt ggctcgactg gaggtcatcg agatcgaccg cttcggcaac gtcgcactgg
     7861 ccctcggtct cgaccggctc tcgctgcgcg actcctgcga gttggcgttg accgtcgaag
     7921 aagagaccga acaggaatgg cgcagtcgtc tcgtgcgcac cttcagcgac ctcaagccgg
     7981 gcgaactcgg tatcctgcag gactcctggg gtcgcgccag cctcgccctc aacggcgcga
     8041 gcgcagcgga gttgctgggc gcccgcctgg gcggcgtggt ccgcatctcc ttcgagaccg
     8101 actgcgcagc cgcgggaccg ggacccgatg ctccggctcg ggctccagcc gacactccag
     8161 cccacgctcc ccttggcgcc gagcggcgct cgtgactgcg gtggcggaca cagcgggtga
     8221 cagactcacc gcgggagacg gactcaccgc ccgcgacaga ctggccctct atcgtcccga
     8281 tctgacccag gtattgagcg cggccgacgc gccccactac cgctacgccc aagtctacga
     8341 acacctcatg cggcgacccg aggcgcccct gtcggcggcc agcaacctgc cgctggcgtt
     8401 gcgaacggcc ttggaagagg ccggttcgtg ctcgctcacg ctcgagaagc ggcgggagga
     8461 cgcggagggc accactaaag tgctgctgac cgcccgcgac ggcgcccaga tcgaagcggt
     8521 ggcgatgcgc tacaacaggc gggtgacggt ctgcctctcc acgcaggtag ggtgctctct
     8581 cgggtgcacc ttctgctcga ccggagctat gggattcgcg cgcgacctga ccgtcgctga
     8641 gatcgttgat caggtgcgcc tcttcatccc cctgctgcgc gaggagagcc gcgagatcac
     8701 caacatcgtc ttcatgggta tgggcgagcc cctgctcaac ctggacgccg tactcgcggc
     8761 gatcaggctc ctcaaggacc cagacggcct cgggttcgcg cagcggtcgc tctcggtgtc
     8821 caccatcggc atcccggccg ggatccggcg gctggcccgc gaggagcccc aggtgaacct
     8881 cgccatctcg cttcacgccc ccaacgacga gttgcgcacc cgtctgatcc cggcgaaccg
     8941 ccgcttcccc ttgagcgagg tgctcaccgc ggcagacgac cacttcgccg tcacgcaccg
     9001 caaactcttc gtcgagtaca tcctgctgga gggagtgaat gacacccgcc gccacgctga
     9061 ggctctggcg cgactcctgc gcgggcgggt gttgacggtg aacctcatcc cttggaaccc
     9121 gggctgcggc gactacgagc cgtcacagcc ggaaaccgcc aacgcttttc gcgacaccct
     9181 gcagacgaga gggctggagg ctactctccg gcttggacgc ggccgagcca tcgccgcggg
     9241 gtgcgggcag ctggccacca agaccgctgc acggggcggc gcgaagcgcc gcacccggag
     9301 gcccacgtag ggcgaaggtc ggctctgact actcctggtg gtcggcgccg gcacctgtcg
     9361 agtcagaggc gtcgtcctac ctcgatctgt gactgagact gccttcgccg gggtcattcc
     9421 ctcgaagacc cccatacacg gagcggcacg tgggagaccc cttcttggcc tccaacaaca
     9481 cctccgagcc ctcggcgcag tgctccgatc tctcgagtcc tcgcagatag gcttctggag
     9541 gcacgagcag cgcgtccacg tcctggccgg gggccggcag catctccacc aaccatgcga
     9601 ctccgtaggg gtcgtcgtcg aagagctcag gttcgtcgac gaccttctgg ttcaccgcca
     9661 ggatcgtgcc cgacaagggt gccccaacgt agcggaccgc tttgaacgtc tcgaagaaca
     9721 ccagatcgtc gcccgcggac acgacgtcac ccggctccac gaagaactcg acggactcga
     9781 tcccccccat cagcagcacg cccaccttgc tcaccccgat acgcacgccc tcagcgaggg
     9841 actgcaccca cagctcctcc tccgcgtcat aggagcggtc gccagcgatc tcgaccgtct
     9901 gtccgtagaa gccttggaga gtcagcatca gtccccctgc ccgctcgcgg ccaaagcggc
     9961 cgcgaccacc cgtctcagct catcctgctc cggaatgccc gcggagacca gccggcactc
    10021 caccaggacg cagggcacgg gcaggtgctc tccggcgagc cttctcaact cccgatagcg
    10081 ctccgcccct gcggcttcct tgagggtgac gacctccact tccaccgctc cttcaccgaa
    10141 ctccgcggcc acctccctgg tggcctcgat ggcgaagagt cagggaacgc agtgcccacc
    10201 ttcggagacc acctgcactg ccagcatcag ctcgccacct cccgcaacac ccggtgtgct
    10261 agcactcgaa gccttcgagt tcctcgtact ctttgatctc ttcctccagg cgccgtcgca
    10321 gctccggatc cttctcaagc ttgaacgcgc acatccccga gcagtggaag cagcccgctt
    10381 ccatctcctc gtacccgaga gcctcctcga tatcgaacga ctcgtcgtgc aggccgtcgg
    10441 ggctggggct gatcgcctgt ctctcgaagg tgtactcgac gtcctcgagc atgtggcggg
    10501 ctatcactgc cgcccgctcg gcgagatcct tagccagtct ctgtccggag acctcgggga
    10561 aggggcgccg gcgaccgatc gcgttcttcg tctccgagcg aacacgcggg gtgaagagtc
    10621 gcgccgcggg cttgatgccc acgaggttca gggcattcga tccgcaccga tgcccgcatc
    10681 cgtcgagcgc gatcacggga taggcctgta cgagttccag cgaatcgggc acccctgtta
    10741 tcaccgaggg gatggagacc aactccactc gtccgggcag gaggtcgtcg gcgaggatgt
    10801 agcccgcgag tcgcgtcacc gacgacaccg gatcgccgat gccgccgcac gggaacaccg
    10861 tcaccttctc aaccgccatc ggtgacctcc tcccctgcgc cgccatcccc gacctccccc
    10921 gcgacgcggg cagacccctg aacggcaggg gcgggatcgc cggcagtgcc ggcggactcc
    10981 tcgggcagcg gaggcatggt ggccggtcgg tagacgcccg gctcgatccg gacgaactcc
    11041 atgacgccct ccatgttcgg cgcggagcgg aaccacttct ccggctgctt cttcaactcg
    11101 gggcgctcga aacggtaggg actgccgctc tccatcatgc cggcggcgat gccggccacc
    11161 cgctcggcca cgcgccggct gaaatccttg gtgacccccg acggatagcg ccggctcccg
    11221 cccgacaggt catagccctg cgcctcggcg tactcgggta ggaggaaccg caccgcgggg
    11281 gtgattccgg cgaggaagag cagattggtg ccgcagctcc actcgcagcc gtcgagagcg
    11341 atcgaagggc tcttgacgac catgagcacg tcttcctcga ctccgcgcag gaaggcggga
    11401 gcgcacgaga tgagggtctt ctcgggcacc agttcctcgg ccaccacgta ggtggccagc
    11461 cgagcgacgg tcgcgcccac cttggtgatg ccgccacacg catgcatgca gatacgtcgt
    11521 accgccatcg ggcctccgag tccagcgggt ttgactgatc gtcggcccga gcgggccgct
    11581 gctcagaaga tgacggggga acacgtatcg gggtggcggc ggcacgccgc cgccaccccg
    11641 aagcccgaag ggtacgatct tactcgtact cctcctgggc ttttgcgtac gccttcttca
    11701 aggtgtcacg ctgctcatcg gagtacgcgg cgccgatgag ggtctcgtac agctcatcca
    11761 acgtggggat gatgctctcg tagacacact cggtgttcac gcacagagtg ggaagcacgc
    11821 gtcctttgcg ctccatgaac atcccgatac cctcggggtt cttgatcgac cactccttgt
    11881 actcgatgaa ttccttgaca tcatccggca acgtgctgag tacatcgacc atgtacccgc
    11941 aggccggtca ctgaagtgag tcaaggctca ggacctcgac gaggatcttt tcagccatta
    12001 tctcttcacc cccccacttt ggccgggctg cgcatgggct gcagcgccgg gtaggggacg
    12061 tcgggatagt gagcatagta cgtctcgtac caggtcttga cttcaaggac cttatccacg
    12121 ttctcaactg gaacgtcgaa aggcatgtct cagccagggg agaaggtgta tcccaccttc
    12181 ccgccggccg ccaggctgac tatcgcgtct tcgcggggat cgatgatccc cagggagagc
    12241 gccagggtca gcttgaggtt accgccgaaa ccgcggccgt acttcagggc gcagtcgcga
    12301 acgtagttca tgttgagctg ctcgtctacg catacgcagg acgcacccgt gtacatcatg
    12361 ttctcgacga tcttggtggc gtctccacag atgtagagag cactgacctt gccggccttg
    12421 tggatcacgt caagagctgg ctgacaggtc gggatcacga acttgtcgta cgtttcgggc
    12481 cgcacctggc tgcctaccgg ctcgactatc gcgacgatgt cgcaaccgat ctcctcggcg
    12541 tagatacgcg cggactccgc gcagaccagg gccgcgaact ccatgatctc catggccaat
    12601 tcaggcttct tcaccatgtc ggtgaagatc ttggctccac gtaggtggga ggccaacgtc
    12661 attggaccac agaccagacc gatcagggcc acatcaccaa gttgcgcctt ggccttcttt
    12721 ccggcctcga tgatgaccgg ccaacgaccg tcgttgggac ccggcacttt gaggttggct
    12781 tccgccaggg tcttgtcgat aagtgggtgg acaataaccg ttggcgggtt atcgtcgaac
    12841 cacttggcct cgatacccat cgacatggct tccacggcca ggtcgaacac aagcgggata
    12901 ccgtcggcct tgtatcgttc ggccgcgtta accacggcct tggcaagcag atcggagtcc
    12961 tgaagaacgg tggttgccgt ctctccgatc aagaatgccc cgtgcacgcc tacataggga
    13021 acccacggta cgaccggggt gtactttccc aagtaggcat cgatgactgc ctgtttaccc
    13081 attgtcctcc ctacacttcc tcgcgagttt ggggacgaat gagactcccc ctgaccatgc
    13141 cccttcccga ccacctccct tcagtgggac tccggaaccc ggtcccgctc gtcactagaa
    13201 cccggccttc ccgaccgggc tcagtcttct accacaatcc cggcaagtct agcc
//
LOCUS       DASRMJ010000105         4251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_297402, whole genome shotgun sequence.
ACCESSION   DASRMJ010000105 DASRMJ010000000
VERSION     DASRMJ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297402"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFD74_03095"
     CDS             <1..861
                     /locus_tag="VFD74_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZK48571.1"
                     /translation="VMLRIGRATGRSNEHRGFHAPGTTGPFGAAVACALLLDLDTDRL
                     LNAIGIAGSLASGLRQFSVSKTGSMVKRLHFGRAAESGVLAASLAAEGIDGPHDILEG
                     NAGFLKVFCDHYDVSELTRGLEGDTFLVREIAMKRFATHGSSQIPLQALLDVRARHIF
                     SADEIESIHVAAAREAVEHHDNPAPTDLMQAQYSVPFCIALACVRDARVRYEVGEITD
                     KRTCEMTVTLRSGAVHKRTLPEPGIPWRPANRDETYEKYAILMRDCARSKVDELFERI
                     QTMDTQARVD"
     gene            complement(898..1971)
                     /locus_tag="VFD74_03100"
     CDS             complement(898..1971)
                     /locus_tag="VFD74_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK48572.1"
                     /translation="MERSDDDDNGAGMTSDPPADSGMTPDLSADAGMPAGRPDDAGMP
                     AAPRAGLVGPIGLVDVHCHWFVFDHPSPRIGSELQDLERAGYSGLVVMPLGGLGLPPE
                     KVPLLLPPAYRDATGLRGGRGWRLDDVDSLLEFQADWTAQEHSLEVIPFLDVRGWDGE
                     TDLTPLFTEGPNAAGTRAGGMRGIKNILVLEEDGPKSGNSALRHVHGLSRDDYLLLHK
                     RCFALAEQFDVPLVYHVDLTLHGDFVLDCLRAHPTVRVDIPHFGLSRRLMAGFFEEFP
                     LLFSDIASLETHIEAGPDSYGAFFRRFGDRIMLGSDSVVAFGLDRLPRYAQVVRDLML
                     PPTVEQAILSGNAGRFLGAAPAR"
     gene            2204..2668
                     /locus_tag="VFD74_03105"
     CDS             2204..2668
                     /locus_tag="VFD74_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HZK48573.1"
                     /translation="MPTPTITHLERRKIEAGVLIPMVQAFQRAIGVEHANEVAREVIL
                     RLARKDGEAWAEQFGRGLTGIEGVFGIAAAGGSLELERTEGSADELRFDVKQCGYADF
                     FNALGLPELGYMFCCSRDSAMAEAFGGDITLERAHTIMEGANSCDFRFVRRG"
     gene            complement(2755..3042)
                     /locus_tag="VFD74_03110"
     CDS             complement(2755..3042)
                     /locus_tag="VFD74_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HZK48574.1"
                     /translation="MIPENRIPTHPGVILGQEFLAPLGISQVAFAAHLGVPVQRINEL
                     INGKRGITPETAWLLAAALDTTPEFWINLQTAYDLARSRPSEPVQRLAAVS"
     gene            complement(3053..3346)
                     /locus_tag="VFD74_03115"
     CDS             complement(3053..3346)
                     /locus_tag="VFD74_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZK48575.1"
                     /translation="MIVSFGDRATEDLFHDRPTSRARRFSQDVVALALVKLDMLNSAA
                     AVLDLRSPPGNRLEALKGDLKGLHSIRVNDQWRLVFRWDGNNASDARLMDYHK"
     gene            3733..3870
                     /locus_tag="VFD74_03120"
     CDS             3733..3870
                     /locus_tag="VFD74_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48576.1"
                     /translation="MPGVAALAAGGFAYSRHGRNALDGEALIELGFTYEGVGVSRSFA
                     T"
     gene            complement(4031..4243)
                     /locus_tag="VFD74_03125"
     CDS             complement(4031..4243)
                     /locus_tag="VFD74_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZK48577.1"
                     /translation="MHRYETIIYWSNEDHLFIADVPELSGCMAHGDTPQDALAQAEEA
                     VALWIETAAEFGDPVPEPRGRRLLYA"
BASE COUNT          777 a         1403 c         1361 g          710 t
ORIGIN      
        1 gtcatgctcc gcatcgggcg ggccaccggg cgttcgaacg aacaccgtgg gttccatgct
       61 cctggaacca cggggccgtt cggggcggcg gttgcatgcg cgttgctgct cgacctcgac
      121 acagaccgtc tactcaatgc catcggcatc gccggatcgc tggcaagcgg cctgcggcaa
      181 ttctccgtct ccaagaccgg gtcgatggtg aagcggctgc atttcgggcg cgcggccgag
      241 agcggcgttc ttgcggcgag tctggcggca gaaggtatcg acgggccgca cgacatcctg
      301 gagggcaatg ccgggttcct gaaggtcttc tgcgatcact acgacgtgag cgaattgacg
      361 cgcggcctgg aaggcgacac ctttctcgtg cgcgagatcg ctatgaagcg cttcgccacc
      421 cacggatcgt cccagatccc ccttcaggcg ctgctcgatg tccgcgcacg tcacatcttc
      481 tccgccgacg agatcgagtc tattcacgtc gccgccgccc gcgaagccgt cgaacaccac
      541 gataatcccg cgcccacgga tctgatgcag gctcagtaca gcgtcccgtt ctgtatcgca
      601 ctggcctgcg tccgcgacgc gcgcgttcgc tacgaggtcg gggagatcac cgacaagcga
      661 acgtgcgaaa tgaccgtcac acttcgaagc ggcgccgtcc acaagcgaac gctccccgag
      721 ccggggatcc cctggcggcc ggcaaaccgc gacgagacat acgagaagta tgcgattctg
      781 atgcgcgatt gcgcccggtc caaagtggac gaactcttcg agcggattca gaccatggac
      841 acccaagccc gtgtcgactg actcaggctt tgaggtcagg tccggcggtt cgagagccta
      901 gcgggccgga gccgccccca agaagcgccc ggcgttgccg gacaggatcg cctgttcgac
      961 ggtcggcgga agcatgagat cgcgcaccac ctgcgcgtat cgcgggaggc ggtcgagccc
     1021 gaaagcgacc acgctgtcgc tccccagcat gatccggtcg ccgaaccgcc tgaagaacgc
     1081 gccgtaggag tcggggcccg cctcgatgtg cgtctccagg ctggcgatgt cggagaagag
     1141 cagcgggaac tcctcgaaga agcccgccat caaccggcgg ctcagtccga agtgcgggat
     1201 atcgacgcgc accgtcgggt gggcccggag gcagtcgagc acgaaatcac cgtgtagagt
     1261 gaggtcgacg tggtacacca ggggaacgtc gaactgttcc gccagagcga aacaccgttt
     1321 gtgaagaagg aggtagtcgt cccggctcag accatggacg tggcgaagag cggagttccc
     1381 cgatttgggc ccatcctcct ccagcaccag gatgttcttg atgcccctca tgcccccggc
     1441 cctggtgcct gccgcgttcg gaccctcggt gaacaaggga gtcaggtcag tctcgccgtc
     1501 ccagccgcgc acatcgagga aggggatcac ctcgagagaa tgttcctgcg cagtccaatc
     1561 cgcctggaat tcaagcaagg aatccacgtc gtccagccgc catccgcggc cgccccgaag
     1621 tccggtagcg tcccggtagg ccggcggcaa caacaggggc accttctcgg gcgggagacc
     1681 gagcccgccc aagggcatca ccacgaggcc tgagtacccc gccctctcca gatcttgcag
     1741 ttcgctcccg atgcgcggcg aggggtggtc gaagacgaac cagtgacagt ggacgtcgac
     1801 gaggccgatg gggccgacga ggcccgcccg aggggcggcg ggcatccccg cgtcatccgg
     1861 tcggccggcg ggcatcccgg cgtcggccga aaggtcggga gtcatcccgg agtcagccgg
     1921 tgggtcggaa gtcatcccgg ccccgttgtc gtcatcgtcg ctccgctcca cgccccgcta
     1981 ctccttgtcg ctacccgtgt ctcgccccgt tccgccgccg cgcacagctc ctgccggcgg
     2041 ccatgagcgg ccgcccttgc gaccgccggg gaatacagta gcaggtgccg gcggtcgtct
     2101 tcggcggcct gccggcggca ggccgcccgg tgtaccctat ccgcaggcgc ggcggatgca
     2161 gccgacgcac ctgccgacag cgcgacaaca cagggagaca ccgatgccga caccgaccat
     2221 cactcatctc gagcgccgca agatcgaggc gggcgtcttg atccccatgg tccaagcgtt
     2281 ccagcgggcg atcggagtgg agcatgccaa cgaggtcgcg cgagaggtca tcctgcgact
     2341 cgcgcgcaag gacggagagg catgggcgga acagttcggc cgaggcctga cggggataga
     2401 aggggtcttt ggaatcgccg ctgccggtgg cagcctcgag ttggagcgga ccgaaggatc
     2461 tgccgacgaa ctccgtttcg acgtgaagca gtgcggatac gccgacttct tcaacgctct
     2521 cggactcccc gaactcggat acatgttctg ctgcagccgg gattccgcca tggccgaggc
     2581 cttcggcggc gacatcacgt tggagcgcgc ccacaccatc atggagggcg ccaacagctg
     2641 cgatttccgc ttcgtgcgcc ggggttgacg caccaccccc gccccctgcc ttcgtgagcc
     2701 aacgccaacc cgcaccgttc ggcgtattcc accgcgcggc cttctgggtg acgatcaact
     2761 cacggcggca agcctttgga ccggttcaga cggtcgactc cgggccaagt cataggcagt
     2821 ctgcagattg atccagaact ccggcgtcgt atccagagcc gccgcaagca gccacgcggt
     2881 ctcaggggtg ataccacgct tgccattgat gagctcgttg atacgctgca ccgggacacc
     2941 aagatgcgcc gcgaaggcca cctgggagat cccgagaggg gcgaggaact cctggcccaa
     3001 gatgacgccg ggatgcgttg gtatgcggtt ctccggaatc atggccaacc tcctacttgt
     3061 gatagtccat cagtcgcgcg tcgctcgcgt tgttcccgtc ccagcggaac acgagccgcc
     3121 attgatcgtt gacccgaatc gagtgaagac ccttcagatc tcccttcagt gcctcgagcc
     3181 ggttgccagg cggggaacgc aagtcaagaa ccgctgcggc actgttcaac atgtccaact
     3241 tgaccaaggc cagagcgaca acatcctggg aaaaccgacg cgcccgactc gtgggccgat
     3301 cgtggaagag atcctcagtc gcgcggtcgc cgaacgagac gatcatcgaa ccccttttga
     3361 tcaatggtcg atcagcacta taccgctatc ccggtaaccg tgcaagtgtg tagcaactca
     3421 gcgctgaagg taacgtgtta ggtatcagct ggagttcgcc cctaccgact tccctgccga
     3481 gagcgctctt acgcggccgc tcggtctgac atggaatctc ggagcatcag ttcgatattt
     3541 catggtaatc cagtgcatgt gtccgatacc ggcattctgc accgactgct ttcgccgcac
     3601 cggcgcacaa aggggcgagg tgttcttctg gggcgtgcat accggcgccg aactccatct
     3661 gctgtgggtt ctgtgctgag gcgacggcga cccctggccg ttggcggagg gcatcaccgc
     3721 ggttccagct atgtgcctgg cgtcgccgca ttggcggccg gcgggttcgc ctactcccga
     3781 cacggcagga acgcgctcga cggcgaggcg ctcatcgagc tgggcttcac ctacgagggg
     3841 gtaggggtgt cgcgatcctt cgcgacgtag cggacccccg gaacacccac cgagcccccg
     3901 ggaggccaca acgtctccgt agcgcgtagc acagacggtg agcacctgtg cgcgttggca
     3961 cggccaaacg cggaggcaga ccatcttccg tggaaggagg ccggccgcaa tggaggtggt
     4021 atgccgcccc tcaggcgtac agcaggcggc gacctctcgg ctccggtact gggtcgccga
     4081 actcagcggc ggtttcgatc cagagggcca cggcttcctc cgcctgagcg agtgcatcct
     4141 gcggcgtgtc cccgtgcgcc atgcaacccg acagctcagg cacatcggcg atgaagaggt
     4201 ggtcctcgtt gctccagtag atgatggtct cgtatctgtg catcagtcat c
//
LOCUS       DASRMJ010000106         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_85514, whole genome shotgun sequence.
ACCESSION   DASRMJ010000106 DASRMJ010000000
VERSION     DASRMJ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85514"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..898
                     /locus_tag="VFD74_03130"
     CDS             287..898
                     /locus_tag="VFD74_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010900780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HZK48578.1"
                     /translation="MTDKQKPTAESPEPAGAASGSGTGSPRSAAEAPKPGGGSQPAKP
                     SKLAETQDGTVLYNLDPIILKDHWEVDYIHSYAQDSPFFAGLSQKKLLGSRCPACGYV
                     YATPRGHCAQCGVATEWHELPQEGTVHTFTTCYFGGEAFLAETPFTLVLVEFEGADTL
                     FLSRLVGVEDREVSIGMPVRARFLRNSKFKPTDVYFIRADPAS"
     gene            956..2143
                     /locus_tag="VFD74_03135"
     CDS             956..2143
                     /locus_tag="VFD74_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZK48579.1"
                     /translation="MDFDLSDDHRLIRATVREFAEKEIAPRAEEMDREAAFPYDIVAG
                     LADIGLLGVPYPEEYGGSGGDTLAYVLTLEELARVDSSVGITVAAHTSLGTNPIWLFG
                     TEEQKREWIPRLASGKELGAFALTEPDSGSDAGALRTKATLEPGATPGTGEWVLNGTK
                     AFITNSGTDITALAIVAAVSGERPDGGREISAFLVPQDTPGYTRAPAYRKMGWRASDT
                     HELHFEDARISEANLLGRRGRGFAQLLTVLDAGRVSVAALGLGLAQGAYEMALRYARE
                     RRQFGRPIGSFQAVAFKLADMATRLEAARGLVYRAAVAKDLGRPYELLASMAKLTTSE
                     LAVWAASEAVQIHGGYGYMDEYPVSRFYRDAKVLEIGEGTSEVQRLVIARMLGTAGSQ
                     EVM"
     gene            2144..>2442
                     /locus_tag="VFD74_03140"
     CDS             2144..>2442
                     /locus_tag="VFD74_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK48580.1"
                     /translation="MLGTPVVDRDRCKGCGLCVEFCPFGHLRIDAELNVAGYHPADVE
                     TTVDAGGAIPSGAARAAESFEHNFAYWCLRCRYCEVVCPDAAIQVSGEIIGIEGTR"
BASE COUNT          387 a          806 c          854 g          395 t
ORIGIN      
        1 gggcatcgac tacgggctcg acctttcgac ggcgaccgaa gtcccggtga acccgagcgg
       61 cggcctcctg gcctgcggac acccggtggg cgcctccggt ctcatgcagg gcgtcttcgc
      121 gctgtggtag ctccagggtt ccaccgagaa gcacttgggt gacgccaccc tgcaggtgaa
      181 gggcgcgcgc cggggagcca tccactccca cgcgggcacc ggcacctacg tcaccgtgac
      241 catcctcgag cggccctgag cgcgtccgga aaggagagga acgaccatga ccgataagca
      301 gaagccaacg gccgagtccc ccgaaccggc cggagccgcc tccgggtcgg gcaccggcag
      361 ccccaggtct gcggccgagg ccccgaagcc gggcggcggc tcgcagcccg ccaagcccag
      421 caagctcgct gagacccaag acggcaccgt tctctacaat ctggacccga tcatcctgaa
      481 ggatcattgg gaagtcgact acatccattc ttacgcccag gactctccct tcttcgccgg
      541 actctcgcag aagaagttgc tgggctcgcg ctgtccggcc tgtggatacg tctatgcgac
      601 ccctcgtggt cactgcgcgc agtgcggagt cgccaccgag tggcacgaac tgccccagga
      661 gggcaccgtc cacaccttca ccacctgcta cttcggcggg gaggccttcc tcgcggagac
      721 ccccttcacc ctagtgctgg tggagttcga gggggcggac acgctcttcc tgtcacgtct
      781 tgtgggcgtg gaggatcggg aggtctccat cgggatgccg gtgcgcgccc gcttcctgcg
      841 caactccaag ttcaagccca ctgatgtgta cttcatccgg gcggacccag cctcgtgacg
      901 gccgggagcg gggcgtcatg gagctcgaac tcgtgacggc cgggagcgag gtgccgtgga
      961 cttcgatctg agcgacgacc acaggctcat ccgggcgacc gtcagggagt tcgccgagaa
     1021 ggagatcgcc ccccgagctg aggagatgga ccgggaggcc gccttcccct acgacatcgt
     1081 ggccggactg gcggacatcg ggcttctggg cgtgccctat cccgaggagt acggcggatc
     1141 gggcggcgac accttggcct atgtgctgac tctggaggag ttggcgcggg tggactcgtc
     1201 ggtgggcatc acggtggcgg cgcacacgtc gctgggcacc aaccctatat ggctgttcgg
     1261 caccgaggag cagaagcggg agtggatccc gcgcctcgct tcgggcaagg agttgggcgc
     1321 gttcgcgctc accgagcccg actccggctc cgacgccggc gccctgcgca cgaaggcgac
     1381 gctcgaacca ggggctaccc cgggcacggg ggagtgggtt ctgaacggca ccaaggcctt
     1441 catcaccaac tcgggcaccg acatcaccgc gctggccatc gtggccgcgg tcagcggcga
     1501 gcgccccgat ggcggccgcg agatctccgc cttcctcgtg ccgcaggaca ccccgggata
     1561 cacgcgcgcg cccgcctacc ggaagatggg ctggcgcgcc tccgacacgc acgagttgca
     1621 cttcgaggat gcccgcatct ccgaggccaa tctcctcggc cggcgggggc gagggttcgc
     1681 gcaactcctc accgtgctgg atgcggggcg ggtgagtgtg gccgccctgg gcctgggctt
     1741 ggcccagggc gcctacgaga tggcgttgcg ctatgcgcgc gagcgccgcc agttcgggcg
     1801 gcccatcggc tcgtttcagg cggtggcctt caagctcgcc gacatggcga cccgcctgga
     1861 ggcggcccga gggctcgtgt accgggccgc ggtggcgaaa gacctcggcc gaccctacga
     1921 gttgctcgct tccatggcga agctcacgac ctccgagtta gcggtgtggg ccgcttcgga
     1981 ggcggtgcag atccacggcg gctatgggta tatggacgaa tatcccgtgt cccgcttcta
     2041 ccgcgacgcc aaggtgctcg agataggcga gggcaccagt gaggttcagc ggttggtcat
     2101 agcccgcatg ctcgggacgg ctgggtcgca ggaggtgatg taggtgctgg ggaccccggt
     2161 ggtcgatcgc gataggtgca aaggttgcgg tctgtgcgtc gagttctgcc cgttcggaca
     2221 cctgcgcatc gatgcggagc tgaacgtggc cggctatcat ccggcggatg tagagactac
     2281 cgtggacgcc ggcggggcga tcccctcagg tgcggcaagg gctgcggaga gcttcgagca
     2341 caacttcgcc tactggtgcc tgcgctgccg ctactgtgag gtcgtgtgtc ccgatgctgc
     2401 gatccaggtg tcgggggaga tcatcggcat cgagggcacc cg
//
LOCUS       DASRMJ010000107         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_480534, whole genome shotgun sequence.
ACCESSION   DASRMJ010000107 DASRMJ010000000
VERSION     DASRMJ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480534"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFD74_03145"
     CDS             complement(<1..540)
                     /locus_tag="VFD74_03145"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HZK48581.1"
                     /translation="MTDEQGQRASSVGLSVPTSVAPRTWRTVVRDRRLWPAFVLWPAS
                     ILLLYAVARGNYLLFHSVVETAAVCVGVLLFVTTRRLFPLTGNSFLTFLGVGFFWAAA
                     VDFIHVLAYKGMGVFPGGGANLPTQLWIVARALEALVLVVSPVFLVRRLRWTRMFVLV
                     GAIAGAGVFAVFAGWFPACF"
     gene            complement(551..2092)
                     /gene="purH"
                     /locus_tag="VFD74_03150"
     CDS             complement(551..2092)
                     /gene="purH"
                     /locus_tag="VFD74_03150"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZK48582.1"
                     /translation="MKVRRALVSVSNKWNLEPFARGLVDLGVQLISTGGTFKFLAEAG
                     IPVTQVSDVTGFPEIMDGRVKTLHPAIHGGILADRRNHEHMKAIQGLGIEPIDLVVVN
                     LYPFEDVAESRGVADEEVIENIDIGGPTMVRAAAKNYTGVAIVVDPHRYDDVLQELRE
                     NDGALSEGTRRDLAMEAFHHTAHYDFAIANWFSAQSGEFPPYVMWDFKKVQDLRYGEN
                     PHQRAAFYADVDGRKHLLSQIQQLHGAHLSFNNLLDLDSGRRLADEFSLPCCVIIKHN
                     NPCGVALAEDITLAYEKALSCDPVSAFGSVIVVNRAVDEALARSLSANFVEVLFAPAF
                     LEEALEILTKKPDIRLLVDEEHRRNPRGLYDLKRVTGGALIQERDSESERREDVNVVT
                     TRHPTEAEWGDLMFAWRVAKHVKSNAIVFAKDLMTVGVGAGQMSRVDSARIAVEKARH
                     PLAGSAVASDAFFPFTDALEVAVEAGAVSVIHPGGSKRDDDVTRYAEGQGMAMVVTGR
                     RHFRH"
     gene            complement(2106..2744)
                     /gene="purN"
                     /locus_tag="VFD74_03155"
     CDS             complement(2106..2744)
                     /gene="purN"
                     /locus_tag="VFD74_03155"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZK48583.1"
                     /translation="MFRIAVLASGSGSNLQVIIDRLHGRVAGIEVAVVVSNVPGALAL
                     ERAERADIPTASFPLEDYPDREARDLAMADALDAAAVDLVVLAGYMHMLSPRFLARFQ
                     DRVINLHPALLPAFPGVHGIEDALRYGAKVTGVTVHYVDAGMDTGPLIKQEPLPVHDD
                     DTPATLAARIHALEHRVLPRVIELFALGKISPPAPGSRLVKVDDDGISQIVW"
BASE COUNT          544 a         1033 c          988 g          480 t
ORIGIN      
        1 gaagcaggcg ggaaaccacc cggcgaacac cgcgaacact cccgcaccgg cgatcgcccc
       61 caccagcacg aacatccggg tccagcggag gcggcgcacg agaaagaccg gcgacaccac
      121 gagcacgaga gcctcgagcg cccgcgcgac gatccagagt tgagtgggga gattcgcgcc
      181 gcctccgggg aagacaccca tgcccttgta ggcgagaacg tggatgaagt ccacggcggc
      241 cgcccagaag aaacctacgc cgagaaacgt gaggaacgag ttgccggtga gtgggaagag
      301 acgccgcgtc gtgacgaaca gcaacacccc cacacagacc gcggccgtct cgacgacact
      361 gtggaacagg aggtagttgc cccgcgccac cgcatagagg aggagaatcg acgccggcca
      421 gagcacgaac gccggccaga gccgccgatc gcgaacgacc gtgcgccacg ttcgcggggc
      481 gaccgaagtg ggaaccgaca gccccactga gctggccctc tgtccctgct cgtccgtcac
      541 gtcctcccct ctaatgccgg aagtggcggc gcccggtgac gaccatcgcc atcccctggc
      601 cctccgcgta gcgggtcacg tcgtcgtcgc gtttggaccc gcccgggtgg atcacgctca
      661 cagctccggc ctccaccgcc acctcgaggg cgtcggtgaa ggggaaaaac gcgtctgacg
      721 ccactgcgct gcccgccagc gggtgcctgg ccttctcgac ggcgatacgc gccgagtcga
      781 cgcggctcat ctgaccggcc cccactccga cggtcatcag atccttggca aagacgatgg
      841 cgttcgactt gacgtgcttc gccacacgcc aggcgaacat cagatccccc cactccgcct
      901 cggtcggatg gcgggtagtg accacgttca cgtcttcccg acgctcgctc tcggagtcac
      961 gctcctggat cagcgctccg ccggtgaccc tcttcaggtc gtagagaccg cgcgggttgc
     1021 gccgatgctc ctcgtccacg agcagtcgga tatcgggctt cttggtgagg atctcaagag
     1081 cttcttctaa gaatgcgggg gcgaacagca cttcgacgaa gttggcgctg agtgagcggg
     1141 ccaaggcctc gtcaacggcc cggttgacca cgatcacgct gccgaacgcc gacacgggat
     1201 cgcacgagag tgccttttcg taggcgagag tgatgtcctc ggcgagtgcg accccacacg
     1261 ggttgttgtg cttgatgatg acgcaacacg gcagcgagaa ctcgtccgcc aggcggcggc
     1321 ccgagtccag gtcgagaagg ttgttgaacg agaggtgcgc accgtggagc tgctggatct
     1381 gcgacagcag gtgcttgcgt ccgtcgacgt cggcgtagaa ggcggcgcgc tggtgcgggt
     1441 tctctccgta gcgcaggtct tgcaccttct tgaagtccca catgacgtag ggtgggaact
     1501 ctcccgattg cgcgctgaac cagttggcga tcgcgaagtc gtagtgagcg gtgtggtgga
     1561 aggcttccat cgcaaggtcg cggcgggtgc cctcggagag tgccccatca ttctcgcgca
     1621 actcctgaag aacgtcgtcg tagcggtgag gatcgacgac gatggccacg cccgtgtagt
     1681 tcttggcggc ggcgcgcacc atggtgggtc cacctatgtc tatgttctcg atgacctctt
     1741 cgtcggcgac gccgcggctt tcggccacgt cctcgaacgg gtagaggttc accaccacca
     1801 ggtcgatggg ttcgatcccg agtccctgga tggccttcat gtgctcgtgg ttgcgccgat
     1861 cggcgagtat gccgccgtgg atggcgggat ggagcgtctt cacccggccg tccatgatct
     1921 cagggaaccc ggtgacatcc gacacctgcg tcaccgggat gccggcttcg gccaagaact
     1981 tgaacgtgcc gccggtcgag atgagctgga cacccagatc gaccaaccct cgggcgaacg
     2041 gctccaggtt ccatttgttc gaaacggaca cgagtgcgcg gcgtaccttc actgcttccc
     2101 ctctttcacc agactatctg cgagatccca tcgtcgtcca ccttcaccaa gcgcgaaccc
     2161 ggcgcgggcg gactgatctt tcccaacgcg aacaactcga tcacgcgcgg gagcacccgg
     2221 tgctccagcg cgtggatgcg cgccgccaga gtggccggcg tgtcgtcatc gtggaccggc
     2281 agtggctcct gcttgatgag cggacccgtg tccatgcccg cgtccacgta gtgcacggtg
     2341 actcccgtga ctttggcgcc gtagcggagc gcgtcttcga tgccgtgcac tcccggaaaa
     2401 gccggcagca acgccggatg caggttgatg acccgatcct ggaagcgggc caggaatctg
     2461 ggcgagagca tgtgcatgta gcccgccaac accaccaggt ccacagcagc ggcgtcgagg
     2521 gcgtcggcca tggccaggtc gcgggcctcc cggtcggggt agtcctccaa ggggaagctc
     2581 gccgtgggga tgtcggcccg ctcggcccgc tcgagcgcca gagcgccggg cacgttgctc
     2641 acgaccaccg ccacctcgat accggcgacg cgaccgtgaa gacgatcgat gatcacctgc
     2701 agattcgagc cgctccccga cgcgagcacg gcgatcctga acatccgctc cctccgctcc
     2761 ttctccgcac cgacctcccg gccgggcgcg gccatagtag cagagctgag cagccgccgg
     2821 aactctccgg gcccaccgct ccgggcagcg cgggccgcgg cgggcgccga acgcggccgg
     2881 gcgcgcgctg cagcgagcga cggcgatcag gcgactagag gaccccggtc agggacacgc
     2941 gcttgtcccc gcggacgatg ctgcccaggt acatgcactc ctcgcccgcc cgctggagca
     3001 gacgggcggc ccattccgcg ttcgcgaacg ggaccacgat cacat
//
LOCUS       DASRMJ010000108         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_188792, whole genome shotgun sequence.
ACCESSION   DASRMJ010000108 DASRMJ010000000
VERSION     DASRMJ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188792"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFD74_03160"
     CDS             <1..472
                     /locus_tag="VFD74_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HZK48584.1"
                     /translation="CRDFPYPILLKEVGSGLPSAALARIAGTDVAGVDVAGRGGTDWG
                     RVEAVLAGRLPDSPLEELGTATADSLVAAVEIMPPRAVILASGGIRSGVEVAKALALG
                     AHAAGMALPFLRWAVESTERIVEGVRTVHDDLRVAMWYAGARDVAAIRGRARRM"
     gene            complement(585..1520)
                     /locus_tag="VFD74_03165"
     CDS             complement(585..1520)
                     /locus_tag="VFD74_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZK48585.1"
                     /translation="MDISCPLACLCFSAGETVINFNQLRAFHEVARNQGFSAAARALC
                     VTQPAVTSHIRGLESSLGVRLFRRRGRRMVLTEVGALLFLQAREVFELERAMERTVAE
                     VRSLERGVLRVGTTKTYSHHLMPSLMTRFHAAHPGVRMVLDEGSSLDMCRSLLDLRNE
                     LAVVAKVEGVRGVTFIPFRNERVILVAAPTHPFAARESILFQELEGLPIITREEGSGV
                     QALTRACFDQRGMTPDVLVETGNVEFIKEMVEGGEALSFLVESAVAEDLAGGRLVAVD
                     IEDQELMLDVNIAYLEEDTLSPAAAAFLSLLLAEK"
     gene            1875..>2479
                     /locus_tag="VFD74_03170"
     CDS             1875..>2479
                     /locus_tag="VFD74_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48586.1"
                     /translation="MQVLLTLEWDVSPDREDEYSAFVARTFIPKCNEMGLLSVGGFYV
                     EVGRGPRIISVKRVESLDDLARIMSSPAYRALVLQLKELVVGYRSKILRPTGRTRDVP
                     YKIQKGVWKYNQYYDILPGKRAQYAEFITNEYLPMLDSVEYLEVTGGWNVLIGGVSEI
                     IGELTFKDPVDIGALLENGPYRELTHVLRRDYVTNYSSRIM"
BASE COUNT          443 a          770 c          834 g          432 t
ORIGIN      
        1 atgccgcgac ttcccttatc cgatcctact gaaggaggtg ggttccggtc tgccctccgc
       61 cgcgctggcg cggatcgccg ggacggacgt ggccggggtc gacgtggccg ggcggggcgg
      121 aaccgactgg ggccgcgtgg aggccgttct cgcgggccgg ttgcccgaca gcccgctcga
      181 ggaactcggc acggcgaccg ccgacagtct cgttgcggcg gtcgagatca tgccgccgcg
      241 cgccgtgata ctcgcgtcgg gcggcatacg ctccggcgtg gaggtggcga aggcccttgc
      301 tctcggcgcc cacgccgccg gcatggctct cccgttcctg cgctgggcgg tggagtctac
      361 cgaacggatc gtcgaggggg tgcggaccgt ccacgatgat ctacgggtgg cgatgtggta
      421 tgcgggtgca cgggatgtcg cggcgattcg ggggcgggca cgacgcatgt gacccctccg
      481 gggcgggctg gaagcgcgga acagagttgt gatcgcccga agccacccga tgtcggtgcg
      541 cccggcgccg ctacgcgtcg gttcgccccg cgtcgtcgtc cggactactt ctccgccaac
      601 aggagactca ggaaggccgc cgcggccggg gagagcgtgt cttcctccag gtaggcgatg
      661 ttcacgtcca gcatcagctc ttgatcttcg atgtccacgg ccaccagccg gcctccagcc
      721 aggtcctccg cgaccgccga ttccaccagg aaactcagcg cctctccgcc ttcgaccatc
      781 tccttgatga actccacgtt cccggtctcc accagcacat cgggcgtcat gccccgttgg
      841 tcgaagcagg cgcgggtgag ggcctgcacg cccgacccct cctcccgggt gatgatgggc
      901 agcccctcca gctcctgaaa gagaatggac tctcgtgcgg caaaggggtg tgttggggcg
      961 gccacgagga tcactcgctc gttgcggaaa gggatgaagg tcaccccccg cacgccttcc
     1021 accttggcta ccaccgccaa ctcgttgcgg agatcgagga ggcttcggca catgtcgaga
     1081 gagctgccct cgtcgagtac catgcgcacg ccggggtgcg ccgcgtgaaa gcgggtcatg
     1141 agggacggca tgaggtgatg tgagtaggtc ttggtggtgc ccacccgcag cacccctcgc
     1201 tccaggctgc ggacctcggc caccgtccgc tccatggccc gctccagctc gaagacctcg
     1261 cgtgcctgca ggaagagaag ggcgcccacc tccgtgagca ccatccgccg gcctcggcgc
     1321 ctgaacaggc gcacgcccag ggaggattcg agaccgcgaa tatgggaggt cacggcgggc
     1381 tgcgtcacgc agagagcccg agcagcggcc gagaaaccct gatttcgggc cacctcatga
     1441 aatgcacgga gctgattgaa gttgatcacg gtctcaccag cgctgaagca tagacatgct
     1501 aatggacatg agatatccat tgactattgt tggcctacga ccactgggta gtactctaca
     1561 ttatgagtca gcaaagaaac aagcactgga cacggacctt ggcccggcgt agagtgcggg
     1621 tgctgcggcc ggactaggcc gaacgtgtcg gccggggggt cggcacgaag gggaagtgtt
     1681 cggtgggcga gacgtcgagc cgaacagagg gggacacaca tgacggtatc tgcgcgtttt
     1741 cttggcgacc ccgcagggca gatgtgcctc gtcacgatgt cgcctcgggc ggtcggggga
     1801 acgtcaccta tccttccacg gtcccgggag gaccttctcc atacctgtcc gcttgacgga
     1861 aaggaggaag gcacatgcag gttctcttga ccctggagtg ggatgtatct ccagacaggg
     1921 aggatgagta ttcggccttc gtagcgcgaa cgttcatccc gaagtgcaac gagatggggc
     1981 ttctctccgt tgggggcttc tacgtcgagg tggggcgcgg cccgcgcatc atctctgtca
     2041 aacgggtcga gtcactggac gacttggccc gcatcatgtc ctccccagcc tatcgcgcgc
     2101 tcgtgcttca gttgaaggaa ctcgtcgtgg gctaccgcag caagatcctg cggccgacgg
     2161 ggcgcacgcg cgacgtgccc tacaagatcc agaagggcgt gtggaagtac aaccagtact
     2221 acgacatcct gccgggcaag agggcgcagt acgctgagtt catcacgaac gagtacctgc
     2281 ccatgctcga tagcgtcgag tatctggagg tcacgggggg ttggaacgtg ttgatcggcg
     2341 gggttagcga gatcatcggg gagctgactt tcaaggatcc ggtcgacatc ggggccctgc
     2401 tcgagaacgg accctaccgc gaattgaccc atgtcttgcg gagggactac gtcacgaact
     2461 actcaagcag gatcatgag
//
LOCUS       DASRMJ010000109         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_261069, whole genome shotgun sequence.
ACCESSION   DASRMJ010000109 DASRMJ010000000
VERSION     DASRMJ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261069"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VFD74_03175"
     CDS             complement(<1..912)
                     /locus_tag="VFD74_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZK48587.1"
                     /translation="MTRIRVGTCSWTDRTMAAAWYPPEVKSAEARLRYYAERFDTVEV
                     DSTYYGLPSERNSKLWVERTPPGFTFHVKAFGMVTRHGIKPEQLPVTLRNAYDLELDR
                     FGRIVHPPDDLRATVFSWFSSALTPLREAGKLGLILLQFPPYFTAEPRNRDYILQAAL
                     LLAPDPVAVEFRHASWVSDEELPHTLSFLSDHDLAYVCVDEPRLDSPTVLPPITAVTS
                     PTAYVRFHGRNAATWNVRGGSAADRFKYLYSDEELAEWTAPVRRLAEEADTTYVLFNN
                     CYSDYTPRNARQLSTLLDLHPASGWRSN"
     gene            complement(909..>2027)
                     /locus_tag="VFD74_03180"
     CDS             complement(909..>2027)
                     /locus_tag="VFD74_03180"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK48588.1"
                     /translation="IIVALSAPYMGAYADGAGAKRRLLLIYTAVAVVATALLGTVREG
                     MIIWGLLLFGIANIGFQGGQAFYNAFLPEVAREKDLGVASGLGSSLGYIGALISLLAA
                     LPFFWNGFEEANLGNVRLLFVAVAVFFAVFSIPTFAVLRDAPRPVMAKVARAPGTLRR
                     LREDLTHLRRNRAPFRFLVSYLLYMDAITTLMTFVGIYASDTLGLTLPRILSLFLISQ
                     FTAIPGSLALGRVSDRYGPKPTITGVLVLWVVILAIGVQAHGYATLVVMALLAGVATG
                     AIFTVSRSMMALLAPKDRQGEFFGFYAVAGRISAIIGPVLFGTISSLSGNQRWALGSL
                     IILMGAGLIVLRGVPMHPTPEAADDAALDTDAPGGEGP"
BASE COUNT          392 a          599 c          747 g          289 t
ORIGIN      
        1 gttggacctc caccccgagg cggggtggag gtccaacagg gtcgagagct gccgggcgtt
       61 ccgtggggtg tagtccgagt agcagttgtt gaagagcacg tacgtggtgt cggcctcctc
      121 ggccagcctg cgcacgggcg ccgtccattc ggcaagctcc tcgtcggagt agaggtactt
      181 gaagcgatcg gccgctgagc ctccacggac gttccaggtg gcggcgttac gtccgtggaa
      241 gcggacgtag gcggtcgggg acgtcacggc tgtgatcggg ggcagtacgg tgggcgagtc
      301 gagtcgcggc tcgtccacac aaacgtaggc taggtcgtgg tcgctcaaga aggagagggt
      361 gtgaggcaac tcctcgtcag agacccagga ggcgtgccgg aactcgacgg cgacaggatc
      421 aggagcgagg agcagcgccg cctgcaggat gtagtcgcgg ttgcggggct ccgccgtgaa
      481 gtacggcgga aactgcagca ggatcagccc gagcttgccg gcctcgcgca acggggtcag
      541 ggctgaagag aaccaagaga atacggtggc acgcaggtcg tcgggcgggt gcacgatacg
      601 accgaagcgg tcgagctcga gatcgtaggc gttgcgcagg gtgacaggta gctgctcggg
      661 cttgatccca tggcgggtga ccatcccgaa cgccttgaca tggaaggtga agccgggcgg
      721 cgtgcgctcc acccagagct tggagttgcg ctccgaaggg aggccgtagt aggtggagtc
      781 gacctcgacc gtgtcgaagc gttcggcgta atagcgcaga cgagcctcgg cagacttcac
      841 ctcgggcggg taccaggcgg ccgccatggt gcggtcggtc caggagcagg tgcccacccg
      901 gatgcgcgtc acggtccctc tcccccgggc gcatccgtat ccagggccgc gtcgtccgcc
      961 gcctcggggg tcggatgcat ggggactccc cgcaggacga tgaggccggc tcccatcagg
     1021 atgatgaggg aaccgagggc ccagcgttgg ttccccgaga gggacgagat cgtcccgaag
     1081 agcaccgggc cgatgatggc ggatatgcga cctgccacgg cgtagaagcc gaagaactcg
     1141 ccctgacggt ccttcggggc gagcagcgcc atcatgctgc ggctcaccgt gaagatcgcg
     1201 ccggtggcga ctccggcgag cagagccatc accaccaggg tcgcgtaccc gtgggcctgc
     1261 accccgatcg ccaggatgac cacccaaagc acgagcaccc cggtgatggt gggcttgggg
     1321 ccgtatcggt cggagacccg cccgagagcc agcgacccgg gaatggcggt gaactgcgag
     1381 atgaggaaga ggctcaggat gcgggggagc gtgagtccga gcgtgtcgga cgcatagatg
     1441 cctacaaacg tcatcagggt cgtgatggcg tccatgtaga gcaggtacga gacgagaaag
     1501 cggaacggcg cccgattgcg gcgcaggtgg gtgaggtcct cccgtaggcg gcggagggtg
     1561 cccggtgcgc gcgccacctt cgccatcacc ggccgcggcg cgtccctgag cacggcgaaa
     1621 gtggggatgc tgaacacggc gaagaagacg gccacggcga cgaacagaag gcgtacattc
     1681 cccaagttgg cttcctcgaa gccgttccag aagaacggca gtgccgccaa cagcgagatg
     1741 agagcaccga tatagcccag cgacgagccg agccctgaag cgacgcccag gtccttctca
     1801 cgggccacct cgggaaggaa ggcgttgtag aaggcctgcc caccctggaa gccgatgttg
     1861 gctatcccga acagcagcag accccagatg atcatcccct cgcgaacggt gccgagcagg
     1921 gcggtggcca cgacagcgac agccgtgtag atgagcagga ggcggcgttt ggcgcctgcg
     1981 ccatcggcgt aggcgcccat gtaaggcgcg gagagggcca cgatgat
//
LOCUS       DASRMJ010000110         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_44864, whole genome shotgun sequence.
ACCESSION   DASRMJ010000110 DASRMJ010000000
VERSION     DASRMJ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44864"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            787..1776
                     /locus_tag="VFD74_03185"
     CDS             787..1776
                     /locus_tag="VFD74_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007951708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZK48589.1"
                     /translation="MSHLLEPLTIRTLTLQNRLVLPPMASSKARPDGGMSDELLAYYD
                     EKSRGGALGLAITEHCFISEQGRNRVGQPSVADDAMIDGLAALAEVFHGNGVPAVVQV
                     SHAGAASAEPEKHLVGPSDVATPIGSGRAPRALTGEEVADIPCQFAAAARRVKEAGFD
                     GVELHSAHAYLLNQFLSPLTNRRTDRYGGSLENRIRLHIEAIRAVRDEVGPDFLVLLR
                     LGARDYLEGGTVIDDSIEAALRFEEAGVDVLDITGGLTGYTRPGIDEPGYFSELSAPI
                     KSAVSVPVILTGGVTEADQAESLLSRGAADLIGVGRAMLRDSAWAVNAVRTAP"
     gene            complement(1918..>2240)
                     /locus_tag="VFD74_03190"
     CDS             complement(1918..>2240)
                     /locus_tag="VFD74_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZK48590.1"
                     /translation="FDDVLASEGVRVIRTPIRAPNANAFAERWVETARADCLDWTLIL
                     GPRHLDRVLRTYVAHYNQKRPHRGLQLLAPETAAPVAELGVVPHIRRRDLLGGLIHEY
                     EVAA"
BASE COUNT          385 a          729 c          732 g          394 t
ORIGIN      
        1 agcccggcca ccgccttcgc cctcgcggcc gtggtgagcc gcacgtcgtg ccgcagcgtg
       61 gtcgccgggt cggaatgccc gagccactcc tgcgcctgct tcaccccgta ggtctcgtag
      121 acccaggtcc agtcctcaac ccggtagccg gctcccgcgg ccgggtcgag acccccagcc
      181 gcagacccgg gacagcgggg aggtaccaca actcacagta gtacgcgaga gtctcttcat
      241 gactggtcgg cgatccgtcg gttccgtcag ttctgtcaac tcggtcgtgc gctaccagct
      301 cgctggccac agtcgccatc cggcacgaag gcgcagcaaa cgcggcggcc atggtgccac
      361 gacgagcgca gccagaccct ttccgtgtca tcaaagactg agcatttggg ttgcagaatg
      421 gttgcagaac ggggtcgggg cggagggtct ggcgggagaa atccctgcaa atgagtggta
      481 gcgcatacgg gattcgaacc cgtgttaccg ccgtgagagg caccccttcc gaggctcaga
      541 taggctcccg aaaggggatt ggtctagcga gtcagcacga ttgccaccgt ctagtcccct
      601 cgccaaccgg ggacttgggt tgctcagtct tcccccggcg tcgacagggc gcaccaggat
      661 tcgaacctgg gccctcttcc ttctcaggca ggctgtcttg ccgaatggaa ccggccttct
      721 tgttctcgtg gcactgaggc tcgcgggtat actgaaaagg ccgcctccaa gagaccaagg
      781 agccccatgt ctcacctgct cgaaccgctg accattcgca ctctcaccct acagaatcgc
      841 ctcgtattgc cgccgatggc gtcatcgaag gcgcgtccgg acggcggcat gagtgacgag
      901 ctgctcgcgt actacgacga gaagtcgcgc ggcggagctc tcggcctcgc gataaccgag
      961 cactgcttca tcagcgagca agggcgcaat cgcgtcgggc agccgtcggt ggcggatgac
     1021 gcgatgatcg atggacttgc ggctctcgcc gaggtcttcc acggcaacgg cgttcccgca
     1081 gtcgtgcagg tcagccatgc gggtgccgcc tcggccgagc ccgagaagca tctcgtcggt
     1141 ccctcagacg tcgccactcc gatagggagc ggtcgggcgc ctcgggcgct cactggtgaa
     1201 gaagtcgcag acatcccctg ccagttcgcg gcggctgcca ggcgagtgaa ggaagccggc
     1261 ttcgacgggg tggagctcca ctccgcccac gcgtacctgc tgaaccagtt cctctcgccg
     1321 ctcaccaatc gccgtaccga ccggtacggt ggctcgttgg agaatcgcat ccggctccac
     1381 atcgaggcga tccgggcggt gcgagacgag gttgggccgg acttcctggt gttgcttcgg
     1441 ctgggcgctc gcgactatct cgaaggcgga accgtcatcg atgacagcat cgaagccgcc
     1501 ctccgtttcg aggaagctgg cgtcgacgtg ttggatatca ccggcggtct caccggttac
     1561 actcgtcccg gcatcgacga gcccggctac ttctcggagc tctcggctcc catcaagtcg
     1621 gcagtctcgg tgcccgtcat cctcacgggg ggcgtcaccg aagcggatca ggccgagagt
     1681 ctcctctcac gaggggcagc agacttgatc ggcgtgggcc gagcgatgct gcgggattcg
     1741 gcatgggcgg tcaatgcagt tcggacggcg ccgtagtctg agggggtttc cgtgccaact
     1801 acgctcaggc caagagagcc ggcagcgcat acgggagtcg gacccgtgtt accgccgtat
     1861 gcccgcggtc acgcaggcga tgcgttcacg atcaccgcgt ggggtgcaaa tactcggtca
     1921 cgcggcaacc tcgtactcgt ggatgagtcc accaagaaga tctcgtcgcc ggatgtgcgg
     1981 cacgacgccg agctccgcga ctggcgcagc cgtctcggga gctaagagct gcagccctcg
     2041 atgcggtcgt ttctggttgt agtgcgcaac gtaggtccgc agaacccggt cgaggtgccg
     2101 ggggccgagg atcagcgtcc agtccagaca gtcggcgcga gcggtctcca cccagcgctc
     2161 ggcgaaggcg ttcgcgttgg gggccctgat cggtgtgcgg atcacccgca cgccttcgct
     2221 tgcgagaaca tcatcgaagg
//
LOCUS       DASRMJ010000111         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_227089, whole genome shotgun sequence.
ACCESSION   DASRMJ010000111 DASRMJ010000000
VERSION     DASRMJ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227089"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFD74_03195"
     CDS             <1..297
                     /locus_tag="VFD74_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48591.1"
                     /translation="VLCALILVAERVGLWEPALLGETLIWFFLAGAVLFFRVMDAAKD
                     THFFRRRALETVGAGAFVEFFVNLKTMPLPWELVLQPAMVIFCPTPPRRPVLHS"
     gene            436..2346
                     /locus_tag="VFD74_03200"
     CDS             436..2346
                     /locus_tag="VFD74_03200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZK48592.1"
                     /translation="MQARGAWLGRVPVWFKLGTILGVSIFLLEMIIMEVKFLPTGDVG
                     AMIDAALLTAFVSPIAYLTHSRPLATQIAERDLARAELVQTHARPEDLSHGLERRVAE
                     QTEHLVAVNHTSTALSHCLTTDEVLQTGLGLVCEATAVDGGVLWLKRGSEVELAARRN
                     LDETIVGQLDRLTSVCPEMRHALALRAPSLLDSSTIPALDDVLGERSSPGARLLTVPL
                     MSRDSVLGALELVLPTGTQCDEAKLQLSEAIGAQLGVAIENAGQYDRARYLAERDSVT
                     DLYNHRSLHSRVEQEFNRAQRIGGIFSLVMMDLDGFKLLNDTYGHPTGDDVLRVVASL
                     LSATVRDSDIVGRYGGDEFMVVLPDTGAQGALDLAQRVRNALIERPYPAPGSEGIPLH
                     VSFGIASYPADARHVNELIGLADGNLYNSKQRGGDMITAAEESEEDRHGQGSGMFGVL
                     DALVTAVDNKDRYTRQHSDDVTRFALTIAQQLSLSDVSLRTLRIAGLLHDVGKIGVPD
                     RILRKPGPLTADEADVVKQHAMLSEIMVKGVPNLTEVMAAVGSHHERWDGAGYPRGLK
                     GAKIPFLGRILAVADAWSAMTTDRPYRTAMTPAAAREEILRGSGSQFDPTIVPAFLAG
                     LDADVELLPAVG"
BASE COUNT          492 a          878 c          914 g          527 t
ORIGIN      
        1 gtcttatgcg ccttgatctt ggtcgctgag cgtgtggggc tatgggagcc agcgcttctc
       61 ggtgagactc tgatttggtt ctttctcgcc ggtgcggtcc tcttctttcg cgtcatggac
      121 gccgcgaaag acacccactt cttccggagg cgtgcactgg agaccgtggg cgccggcgcg
      181 ttcgtcgagt tcttcgtcaa tctgaagacg atgcccttgc catgggagtt agtgcttcaa
      241 cctgcgatgg tgatcttttg ccccacccca ccccgaagac ctgtattgca ctcgtgagga
      301 gacagttcta ggagctaata aattgatgac cgttggccga taacgaatcg atccctttgt
      361 cgtcaagcgg aggcgcggtt ggctcacggc ttgcgtcggt agttcgcagg caaagggccg
      421 accttgggag gtctcgtgca agctagaggc gcatggctcg gcagggttcc ggtttggttc
      481 aagcttggca ccatcctggg cgtctccatt ttcctgctcg agatgatcat catggaggtc
      541 aagttcctgc cgacgggcga cgtgggtgcg atgatagatg cagcgctgct cacggcgttc
      601 gtgtccccca tcgcctatct gactcattca cgtcctctgg ccacccagat tgcagagcgc
      661 gacttggcgc gggcagaact tgttcaaacc catgcccgac ccgaggacct ctcacatgga
      721 ctcgagcggc gggtggccga gcagaccgag catctggtcg ccgtcaacca tacgtccacc
      781 gccctcagcc actgcctcac gaccgacgag gtgctgcaga cgggtctggg gctggtgtgc
      841 gaggcgaccg cggtcgatgg cggagtgctc tggctgaagc gaggcagtga ggtggaactg
      901 gctgccagac gaaacctcga tgagaccata gtcggtcagt tggacaggct cactagtgtg
      961 tgcccggaga tgcgacacgc actggccttg cgcgcgccca gcctgctcga cagttcgacc
     1021 atacccgccc tcgatgacgt tcttggcgag aggagcagcc ctggcgctcg gctcctcacc
     1081 gtgcccctga tgtcacgtga ctctgtgctg ggcgctctcg agctcgtcct gcccacggga
     1141 acgcagtgcg acgaagcgaa gctgcaactc tcagaggcaa tcggagccca gctgggcgtg
     1201 gccatcgaga acgcagggca gtatgaccgc gcgcgctacc tagccgagcg ggactcggtg
     1261 accgatctct acaatcaccg ctccctccac agccgtgtgg aacaggagtt caaccgagca
     1321 cagcggatcg gggggatctt ctcgctggtc atgatggacc tggacggctt caagctccta
     1381 aacgacacct atggacatcc aaccggcgac gatgtgctgc gcgtcgtagc ctccttgttg
     1441 tccgccaccg tgcgcgactc cgacatcgta gggcgctatg gtggcgacga gttcatggtc
     1501 gtcttgcccg atacgggcgc ccaaggcgcg ctcgacctgg cccaacgtgt ccgcaacgcg
     1561 ctcatcgagc gtccctatcc ggctccgggc agcgagggca tcccactgca tgtcagcttc
     1621 ggcatcgcga gctacccggc cgacgcgcgg cacgtgaatg agctcatcgg cctggccgac
     1681 ggaaacctct acaactcgaa gcagcgtggc ggcgacatga tcacagccgc tgaagagagc
     1741 gaggaagacc gacatgggca gggcagcggg atgttcggag tcctggacgc tctggtgact
     1801 gcagtggata acaaggaccg ctacacgcgt cagcattcag atgacgtgac gcggttcgcc
     1861 cttaccatcg cccagcaact gtctctgtcc gatgtgagcc tgcggaccct gcgcatcgcc
     1921 ggcctactgc atgatgtggg caagatcggc gtacccgacc gcatcctccg caagccgggc
     1981 ccactgaccg cggacgaggc tgacgtcgtg aagcaacatg ccatgctgag cgagatcatg
     2041 gtgaagggag tcccgaatct caccgaggtc atggccgcgg tcgggagcca ccacgagcgc
     2101 tgggacggcg ccggctaccc gcgcgggctc aaaggcgcga agatcccatt cctcgggcgg
     2161 atcctggcgg ttgcggacgc gtggtcggcc atgaccaccg atcggccgta tcgcacggcg
     2221 atgacgcctg cggcggctcg cgaggagatc ctgcgcgggt cgggatccca gtttgacccc
     2281 acgatcgttc ccgccttcct cgcgggcctc gacgccgacg tcgagttgct gccggccgtg
     2341 ggctgacgca caccctcgcg aggcttctgc cgccctccag accagggaag cgaggagcac
     2401 acgcaagagg cggatcgtgc gcccgcagtt tctcccgacg ccggccgcgg caagctcgcc
     2461 agcctgtctc gtagggcacg gggctgcttc agggggcagc acgatgagca ccgtcgtggc
     2521 cgtcccgcat ccacgggagc gtctggaact ctgtgggggt ctggtcgggg gcgaccatcg
     2581 ggatggcact gatagtggac gctctgacct agacgtggtt cactgagccc gatagggccg
     2641 cgcggcggat gcgccgtagg cggaatcatc ccgagaggac tggaggtcca tcatgctggc
     2701 cagtttctct gttgctccct ttggtgttgg agaaggactg ggcgagttcg tggcggggat
     2761 gatgccgctg atcgaagcct ccgggcttcc ctttaagctg ggagccatgc a
//
LOCUS       DASRMJ010000112         4286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_448101, whole genome shotgun sequence.
ACCESSION   DASRMJ010000112 DASRMJ010000000
VERSION     DASRMJ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448101"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFD74_03205"
     CDS             <1..937
                     /locus_tag="VFD74_03205"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZK48593.1"
                     /translation="TGRAGEGSRAASTFAIFEQVQRDLVGRTHEVREIFAAILSRKHI
                     LLEGPPGTSKSTIVRSIARSMNVPFYFIEGSIDLTPSKLLGFFNPGKVLKDTYLRDYF
                     EMGPLTQAMEEGGVLYLEEFNRMSSESANLLITPMEEGVINIPRYGQVTAKDGFTIIC
                     SQNPYDDVGTLQVSRAFLDRVVRVRMDYQSEDEEIAIVKLRAGIDDHQLAKTATRITR
                     LTREAHEIKLGASVRAAIDIVVIVKNLTLLTDGDLRMEDFAAACKMALSSKVWLSETV
                     NATPEEVIDRVIEKARAELGHEFYLGLGGDKRKAL"
     gene            1009..1815
                     /locus_tag="VFD74_03210"
     CDS             1009..1815
                     /locus_tag="VFD74_03210"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HZK48594.1"
                     /translation="MAESINLDPRMQQDFMADEEFLKYFAKLNHLLSSGLRGKAFELA
                     KALIVKLAAQLVGTGVRKGTLHLRRGAFTDEIDLDATLENLVADPLDHLEDNLAVWDR
                     RREQTGFVMMFDHSYSMRGPKIVLAALCTAAIAIHFKKNYGVVAFSTEVKTIKAVSDT
                     LHYEVLLDRVFDLPIEGLTNISGALQTGLRQLESFDKVFGLLLTDGGWTTGENPLPVA
                     ARFDRLNVIAFPPANPEKVRLIAEAGHGTFQFVGDEEGILGAIIRALQTH"
     gene            complement(2084..3490)
                     /gene="mtbB"
                     /locus_tag="VFD74_03215"
                     /pseudo
     CDS             complement(2084..3490)
                     /gene="mtbB"
                     /locus_tag="VFD74_03215"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            complement(3487..4281)
                     /locus_tag="VFD74_03220"
                     /pseudo
     CDS             complement(3487..4281)
                     /locus_tag="VFD74_03220"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
BASE COUNT          761 a         1469 c         1339 g          717 t
ORIGIN      
        1 taccggccgc gcgggcgaag gctcgcgggc agcctccacc tttgcgatct tcgagcaggt
       61 gcagcgcgat ctcgtgggcc gtacccacga ggtgcgtgag atcttcgcag ccatcctgag
      121 ccgcaagcac atcctgctgg agggaccgcc cggcaccagc aagtcgacca tcgtgcggag
      181 tatcgcgcgc tctatgaacg tcccgttcta cttcatcgaa ggttcgatcg acctcacgcc
      241 gagcaaactc ctcgggttct tcaatccggg caaagtcttg aaagacacct acctgcgcga
      301 ctacttcgag atgggtccgc tcacccaggc catggaagag ggcggcgtcc tctatctcga
      361 ggagttcaac cgcatgagct ccgaatccgc caatctgctc atcacgccca tggaagaggg
      421 cgtcatcaac atcccccgct acgggcaggt caccgccaag gacgggttca ccatcatctg
      481 ctcccagaac ccctacgatg acgtcggcac cctccaggtg agtcgcgcct ttcttgaccg
      541 cgtggtccgc gtgcgcatgg actaccagag cgaagatgag gagatcgcga tcgtcaagtt
      601 gcgggccggc atcgacgacc accaactagc caagaccgcc acccgcatca cccggctcac
      661 ccgtgaagcc cacgagatca agctgggggc ttcggtgcgg gcggccatcg acatcgtggt
      721 catcgtgaag aacctcaccc ttctcaccga tggcgatctg cgcatggagg atttcgccgc
      781 agcctgcaag atggcgttgt cgagcaaggt gtggctctcc gagacggtca acgcgacgcc
      841 cgaagaggtc atcgaccgcg tcatcgagaa agcccgcgcg gaactcggcc acgaattcta
      901 cctggggctc ggcggggata aaagaaaagc cttgtagagg tccggggcga gccgggcctg
      961 gcactcctca agcaaagact cgatgccggt gagatcaccg cgttcgacat ggccgagtcc
     1021 atcaacctcg atccccgcat gcagcaggac ttcatggcgg acgaggagtt cctcaagtat
     1081 ttcgccaagc tgaaccacct actgagctcc gggctacgcg gcaaggcgtt cgaactggcg
     1141 aaggccctca tcgtcaagct cgccgctcag ctggtcggaa caggagtgcg caagggcacc
     1201 ctgcacctgc gacgcggcgc cttcaccgac gagatagacc tggacgccac cctcgagaac
     1261 ctggtcgccg accccttgga tcacctcgag gacaacctgg cggtctggga ccgccggcgt
     1321 gaacagaccg gcttcgtgat gatgttcgac catagctact cgatgcgcgg acccaagata
     1381 gtgctggccg cactctgcac ggccgccatc gccatccact tcaagaagaa ctacggcgtg
     1441 gtcgccttca gcaccgaggt caagacgatc aaggcggtga gcgacacgct gcattacgag
     1501 gtgctgctcg accgggtctt cgacctcccc atcgagggcc tcaccaacat ctcgggagcc
     1561 ttgcagaccg gtctgcgcca actggaatcg ttcgacaagg tctttgggct gctccttaca
     1621 gacgggggct ggaccaccgg cgagaaccct ctgccggtgg cggcaagatt cgaccgactc
     1681 aacgtgatcg ccttcccccc ggccaatccc gagaaggtcc gcttgatcgc cgaggccggt
     1741 cacggcacgt tccagtttgt cggtgacgag gaaggcatct tgggcgcgat catccgggct
     1801 ctacaaacgc actgaagggt cggcggggcc gggttcgccg ctcgaccgcg gcggctcggc
     1861 agggtcgcgg ccgagcgact cggtagcgcc gcgcgccgcc ggctcggcag ggcgccggga
     1921 cagcaacccg gcggcagctc agcggttcga gacccgtgct cgcggccctc ggccggccag
     1981 cctcaccagc ccaagtgaca tccgagcgcg aacgccgcga ccaccaccag ggcgagcgaa
     2041 gacagcctgg gcacgactac ttcggccagt atcgacctct gcttcacagc tcggcctcga
     2101 gcttcgctct gagcatgcgc actgatgcga tctcgatgcc caggagtcgc tcgatgcggg
     2161 ccttggccgc gagacctcgc gccgctcccg gcatcgccgt caccacaccg atgtcgagat
     2221 cctcacgcag ttcgcgcata agcacaacgt cagagaggtc gtcgatgccg accctcaact
     2281 tctcggccac atacgccttc gcctgcggga gtcgcatctt gcggtggagt tgcatcctgg
     2341 cgaccagatc tccggccgtg cggatgcccc ccatcccgga ggcgagcacg tgtgccatgg
     2401 gcatccccac gggatcgccc acgcccacct ataacccgtc cacccgcccg atctcgacca
     2461 tcgcggcgct cgcacgagtg accgcgtcca cgggcggcgt ctcgcacatg ggcacgcccc
     2521 ccactcccat gcccacgttg gcgtggatgg ggatcgacgc cgcctcagaa caggccttga
     2581 cgaacgtcac cgcccgcgcc acgttccagg ggcccgactt gcgtgtgttg gtgttgacca
     2641 ccgggccgaa cacgctcgcg ccggccttct ccacgagctt cacctgttgg tgcggataca
     2701 ggcccgcaag gcgctctccg cgaaactcga cctccccgtg catgccgaga acgaactcgg
     2761 ccgccatccc cacctggatg gagaggtgcg tgctcgccgc caactcctct atcgcctgaa
     2821 gggtggcgag gaactcggcg tccccggcgg aggcggtggt gtcgaagttc aagccgtcgg
     2881 cgccggcggc ggccatcttc ttgcccacgt agatcatgtc gcgggtgcag gcctcggcgg
     2941 cttcgagttg tgccgcccgg gcgtcggcga tcctgcccag aggcatgaga tcggagggat
     3001 tgccgaacgg tccgtcgggg cgggcgtacg tggccaagtt gggcatggcc ccgtagtaca
     3061 ccgggatgat cgtggacagg agcacgctct ccatgcactg ctgctcgttg gcgaccacgg
     3121 gcttgaccgg cttgtacgag tagtcgggat ggccgagttc cgcggtgtcg aatccgaagg
     3181 cccgctcgaa gaggcggatg gcctgttccc gcgaaacggg aacgcccacg ccgctgtctc
     3241 cctggtcggt ccacagggaa cccgccacgc cgtcgtcggt gagcacgacc tcgtgcccgg
     3301 gttcgacgcc gacgatgcgt cctcggaacg cgaacaggtc gaacaggtag tctcgctcct
     3361 gttcggtgag cgcgggaata cgcccccggt cggcggcgtc ctccgtgccg gccaggatat
     3421 cggcgcgcaa ctcatcggag gatcgagcct ccacgcgtcc atcgcccatg cgtatgcggt
     3481 gagtagtcat cggtcgcctc tcggactggg ggctggtcgc cccagatgtc tcgctatcgc
     3541 cgcctccgcc tcgggcggca gtgacgccac cgtgtgctcc cccaggatgg cacgcgcctc
     3601 ggccgacgca cgcacgtgga tgtcgtgccc tccggccttc aaccacgcac ccctcatgcg
     3661 ccgatcgatc aaccgggccc gagattgctc tctggcggcc agttcgcgcg tctgcttctc
     3721 gcggaggaag ttcccgtcgg agccgacgcg ccggatcgaa tcgagcccca acgtggtcgg
     3781 gtcggttcgg atgccttcca gcacccgtct gttcatgcgc gctatctcgt tatcgatcac
     3841 catctgaccg aaatcgaagg tcagacccaa ctcgagcatc cccatcccat agatcaggtt
     3901 ggcccctgcc aacgctggta acagactggt gagggtcttc tcgtggcccg cctgcgcgtc
     3961 gggtagcttg ctgtccgcct aaccgccggc cacccagcag ggcaggtaat acagctcgct
     4021 catctcggcg acggcagcgg agacgagccc gagttcgggg gcgccgacgg gcgccgtggc
     4081 gtgccaaaga tcgaaggtgg tggtgctgct gccgtagatg acgggagccc cggccacggt
     4141 gagttgtgca agcgtcaacg ccgagagcac ctcagcgttg tgggtgacga tggtgccccc
     4201 gaggctgatg ggaccggttg ctccggccat caccatggag atcacgttcg aggcgatccc
     4261 gtggcgggcg cagcccatca tgatct
//
LOCUS       DASRMJ010000113         6648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_448393, whole genome shotgun sequence.
ACCESSION   DASRMJ010000113 DASRMJ010000000
VERSION     DASRMJ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448393"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VFD74_03225"
     CDS             <1..1054
                     /locus_tag="VFD74_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HZK48595.1"
                     /translation="QHRRSVERSRRRRRRLQRSAVLLVLVLLAAAAYLGVSRIRWGAG
                     GSAATSTTLAGSAVGDAVLLSITQDDQTRVAVLIAPSAQPTLLVALPGDVLIRASTGF
                     DPLRVFLDGPDEEATFQKAAEGLESMLGTRPVARADIQWSDLLKAVSSVGAGSTAPAD
                     LRSDDAKDAAVVADVLKALLAAVGTAPGDAALAALSVNGDRDAARAALRALGGAAAVS
                     GAVPGRLVEGLGFAYFEPDVAGLQELLGGRPPESAVSVEVQNGSGVVGAAQKVSEVVS
                     PLGYTVLPPKNAEGFPDVAITQLFAASDALAEADRLRGLLGRGTVVQQDTLPPGRVII
                     IVGKDLDTSALPVSGG"
     gene            1057..2091
                     /locus_tag="VFD74_03230"
     CDS             1057..2091
                     /locus_tag="VFD74_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZK48596.1"
                     /translation="MTSGEDPRFSRYQRQMLFAPFGAQGQEKLASARVGLVGCGALGS
                     VIANHLVRAGVGYVRLVDRDFLELNNLQRQMLFTESDVRERIPKAEAAAGHLREINSD
                     VTVDPVVADINPFSIVEFGDGLDLLVDGTDNFTTRFLINDYCVSRGIPWVYGGVIMAS
                     GMTMTLVPGEGHCLRCVFPELPPPGSAPTCDTVGVLNTAVAVVASLEANEVFKLILDP
                     GSRNKGLLTVDLWNLAFETVEVKRDPECPACGRGLFPFLGGDHEESAVSLCGRDAVQI
                     VPVSSRVLDLGALRSRLAGVGPVRANPFLVELDVEGKTITVFPDGRAIIKGTDDIAAA
                     RVLYARYVGQ"
     gene            2189..2623
                     /locus_tag="VFD74_03235"
     CDS             2189..2623
                     /locus_tag="VFD74_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZK48597.1"
                     /translation="MNITSKSKYAVRALVELARRGQDEPVPLMVLAEARGIPGQFLEQ
                     LFATLRRAGILQSFRGVHGGFSFNRPPDQVTVLDVVEALDGPIEPALCTTDESCERRP
                     VCSVGDVWVQAKLSVEEVLSGVTIQDLATRESSLTGTSMYYI"
     gene            2641..3567
                     /gene="larE"
                     /locus_tag="VFD74_03240"
     CDS             2641..3567
                     /gene="larE"
                     /locus_tag="VFD74_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HZK48598.1"
                     /translation="MSIADITTGTAESLRTDSPAGRAESLRTDSPAEAAWDAAPAETV
                     RSLVRSFGSAVVAFSGGVDSSVVLALAVEQLGADRVLAVTSRSETYLEEEFTEAAEVA
                     ARLGVSHHVIETKELGIPGFSSNPPDRCYHCKAELFGELAGIAAERGFETVVDGANLD
                     DMGDFRPGIKAADELGVRHPLMEAGLGKDAVRALARSMGLSNWAKPAMACLSSRFPYG
                     EAITREKLSMVARAEALLRAAGYAQLRVRHHGVTARIELPADEVAAFACDVPLRSRVV
                     AEFKDIGYRYVTLDLEGFRSGSMNEALEGERR"
     gene            3624..4403
                     /gene="cysE"
                     /locus_tag="VFD74_03245"
     CDS             3624..4403
                     /gene="cysE"
                     /locus_tag="VFD74_03245"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZK48599.1"
                     /translation="MTGLGGLMFLYLTKSGAEVRESVRRDIQVIRERDPAARSALEIV
                     TSYPGFHAVLIHRVAHALWVRQVPLVPRLISQLGRFLTQIEIHPGAQIGQGLFIDHGS
                     GVVIGETAEIGDDVTIYQGVTLGGTGKESGKRHPTVGDNVVIGTGAKILGSLTIGDNA
                     KIGAGSVVVQPVPANSTVVGNPGRPVITDGTRVGIPDIDYTHLPDPVAQAMQCIVRRM
                     VQMENELEEICPPHQKRKLEQREVLDEQLCNLTGYSQGSGI"
     gene            4490..>6648
                     /locus_tag="VFD74_03250"
     CDS             4490..>6648
                     /locus_tag="VFD74_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZK48600.1"
                     /translation="MTEPDRSPVSPEPSAAPTATRAPSAVSVVGPGAAGVARVGEGLN
                     GPQAEAVFHEGGPLLVLAGAGSGKTRVLTHRVAHLIQSGQAGPYEILAITFTNKAAAE
                     MRSRVVELVGPEAQNMWVSTFHSACARILRKEAQLLGYRSGYTIYDQDDQVRLMRRCL
                     EELGHDIKRFPPRAVHAQVSDAKNRLVDVDEFQRPGNPGADSWEAGVVGKSGVDYLEV
                     TAAAYRLYQQRLYELNAMDFDDLIMRTVDVFRLFEHRLAHYRNVFRHILVDEYQDTNH
                     AQYVLVKLLAEEHRRITVVGDDDQSVYSWRGADIRNIMEFEEDFGESTIIKLEQNYRS
                     TTTILDAANAVVAHNRGRKPKRLWSERGAGEKVLVLECRDEHEEARLVAGEIQTLLRK
                     GRRAGEVAVFYRVNAQSRVLEDILVRYNIPYQVLGGTRFYERAEIKDLLAYLRVVVNP
                     SDDISFQRMVNSPKRGLGAVAQGRLQLLAAEDETSLRGALPLAADSGDLATAARSAFG
                     ELDGLIRSWEQRLREGASVADQVRAVMEESGMLAALRDARTLEAEGRLENLQEFLGVT
                     QEFDRFNPGGGLIDFLEEISLYADVDSMRDQAELVTLMTLHNAKGLEYPFVFITGMEE
                     GMFPHSRALDEQNLEEERRLCYVGITRAMERVHLSHARNRSLYGTGGYRMPSRFLAEI
                     PRELVELRGAAPVVSRRPGAGYTPAHRYRAGGGAGRGA"
BASE COUNT         1100 a         2113 c         2297 g         1138 t
ORIGIN      
        1 gcagcatcgc aggagcgtcg agcggtccag acgccgacgc cggcgcctgc agcggagtgc
       61 ggtgctcctg gtcctcgttc ttctcgccgc cgcggcctat ctgggggtga gcaggatacg
      121 gtggggcgcc ggcggttccg ctgccacctc caccacccta gcggggagcg ccgtcggcga
      181 cgccgtgctg ctcagcatca cccaggacga ccagacccgg gtagccgtcc tcatcgcgcc
      241 gagcgcgcaa cccacacttc tcgtcgcgct acccggagac gtgctgatcc gtgcctcgac
      301 cggcttcgat ccgttgcggg tgttcctgga cggaccggat gaagaagcga cgttccagaa
      361 agccgctgag gggcttgagt ccatgttggg gacaaggccg gtggcacgcg ccgacatcca
      421 gtggtccgac ctgctcaagg ccgtctcttc tgtgggagcc ggttccacgg ctcccgcgga
      481 tctgcgatcg gacgacgcca aggacgccgc ggtggtggcg gatgtgttga aggcactgct
      541 ggccgccgtc ggcaccgctc cgggcgatgc ggcgttggcg gccctttccg tcaacggcga
      601 tcgagacgct gcccgcgcgg ccctgcgcgc actcgggggc gcggccgccg tgagcggagc
      661 ggtcccgggt cgcctggtgg agggactcgg cttcgcctac ttcgagcccg acgtcgccgg
      721 actgcaggag ctgctgggcg gcaggccgcc cgagtccgct gtgtcggtgg aggtgcagaa
      781 cggctccgga gtggtggggg ccgcccagaa ggtctccgag gtagtgtctc cgctaggcta
      841 caccgtgctg ccgccgaaga acgccgaagg cttccccgat gttgcgatca cgcaactgtt
      901 tgcggcctcc gacgcgctcg ccgaggccga ccgcctgcgc ggactcctcg ggcgcggcac
      961 tgtggtccaa caagatacgc ttccgcccgg tcgggtgatc atcatcgtag gcaaggacct
     1021 cgacacgagc gctttgccgg tgagcggggg atgagagtga cttccggcga agatccgcga
     1081 ttctcgcgct accaacgcca gatgctcttc gcgcccttcg gggcgcaagg ccaggagaag
     1141 ctcgcctccg cccgcgtggg gttggtaggc tgcggggctc tggggtcggt gatcgcgaac
     1201 cacctcgtgc gcgccggcgt gggctacgtg cgcctcgtcg atcgcgactt cctcgaactc
     1261 aacaaccttc aacgccagat gctcttcacc gagagtgacg ttcgcgagcg gataccgaag
     1321 gcggaggccg ccgccggtca cttgcgcgag atcaacagcg atgtgactgt cgaccccgtc
     1381 gtcgccgaca tcaatccgtt ctccatcgtg gagttcggcg atggactcga ccttctcgtc
     1441 gacggcacgg acaacttcac gactcggttc ctgatcaacg actactgcgt gagccgcggg
     1501 atcccgtggg tgtacggcgg ggtcatcatg gccagcggga tgaccatgac cctggttccg
     1561 ggcgagggac actgcctgcg ctgcgtcttt cccgagttgc ccccgccggg gtcggcaccc
     1621 acgtgcgaca cggtcggagt cctgaacacc gctgtggcgg tggtggccag tctggaggcc
     1681 aacgaggtct tcaagctcat cctcgacccc gggtcgcgca acaagggact cctgaccgtc
     1741 gatctgtgga acctggcatt cgagacggta gaggtgaagc gcgatcccga gtgcccggcg
     1801 tgcggccgag ggctgttccc gttcctcgga ggcgatcacg aggagtcagc cgtctctcta
     1861 tgcggacgtg acgctgtgca gatcgtgcct gtgtcgagcc gggttctcga cctcggagcg
     1921 ttgcggagcc gcctcgcagg tgtggggccg gtgcgggcta accccttcct cgtggagttg
     1981 gatgtggagg gcaagacgat caccgtgttc cccgacggga gggcgatcat caagggcacc
     2041 gacgacatcg ctgctgcgcg ggtgctctac gctcgctacg tgggacagta atccggaagg
     2101 tcgggggccg gtcgcccttg acgtaccccg cttcgctggt agaatcgctt aattcatact
     2161 caactactca ggattaggct gcacttctat gaacatcacc agcaagagca agtatgcagt
     2221 ccgcgcactg gtcgagctgg cccgccgggg tcaagacgag cccgtccccc tcatggtgct
     2281 cgcggaggca cggggcatcc cgggtcaatt cctggagcag ctcttcgcga ccctgcgtcg
     2341 ggcggggata ctgcagtcgt tccgtggggt gcacggaggc ttctcgttca atcgtccccc
     2401 cgatcaggtg accgtacttg atgtggtcga ggccctcgac gggcccatcg agccggcttt
     2461 gtgcaccacg gatgaatcgt gcgagcgtcg gccggtatgt tcggtcggcg acgtctgggt
     2521 gcaggcgaaa ctctcggtcg aggaggtcct ctccggagtc acgatccagg atctcgccac
     2581 gagagagtct tcgctcacag gcacgtccat gtactacatc tgacccgcgg agggaatcga
     2641 gtgtcgatcg cggatatcac caccgggact gccgagtcgt tgcgcaccga cagtcccgcc
     2701 gggcgcgccg agtcgttgcg caccgacagt cccgccgagg ctgcctggga cgcggcgccc
     2761 gcagagacgg tccgctcgct cgtgcgctcc ttcgggtccg ccgtcgtggc cttctcgggt
     2821 ggcgtcgact cgtcggtggt gctggcgctc gcggtcgagc agctcggggc cgacagggtg
     2881 ttggcggtca cctcgcggag tgagacctac ctggaggagg agttcaccga ggctgccgaa
     2941 gtggcagccc gcctcggagt ctcccaccac gtcatcgaga ccaaggaact ggggattcca
     3001 ggattctcat cgaacccgcc cgatcgttgc taccactgca aggccgagtt gttcggagaa
     3061 ctggcgggca tagccgcgga gcgcggtttc gagacggtgg tcgacggcgc caatctggac
     3121 gacatgggtg actttcgccc cgggatcaag gccgccgacg agctcggtgt gcggcacccg
     3181 ttgatggaag ccgggcttgg taaagatgcg gtgcgtgccc tggcacggtc gatgggtctg
     3241 tcgaactggg cgaagccggc gatggcctgc ctctcctcgc gattccccta cggggaggcg
     3301 atcacgcgcg agaagctgtc gatggtcgct cgggccgaag cgctcttgcg tgcggcgggg
     3361 tacgcacaac tgcgcgtgcg tcatcacggt gtgacggcgc ggatagagct tcccgcggac
     3421 gaggtcgctg cctttgcgtg cgatgtgccg ctgcggtcgc gggtggtcgc ggagttcaag
     3481 gacatcggat atcggtacgt cacgctggat ctcgaaggct tccgcagcgg gagcatgaac
     3541 gaagcacttg aaggggagcg gagatgagtc acaaggggag tctcgacgcg ggttcgcacc
     3601 ggaagggatc gttgctcacc acgatgaccg ggctgggcgg cctgatgttc ctctatctca
     3661 ccaagagtgg tgccgaggtg cgggaatccg tgcggcgtga catccaggtc atccgggaac
     3721 gggaccccgc cgcgcgcagt gctctcgaga tcgtgacttc ctatccgggc ttccatgcgg
     3781 tgctgatcca tcgcgttgcg catgccctct gggtgcgcca ggtgccgctg gtgccgcggt
     3841 tgatctctca actcggtagg ttcctcaccc agatcgagat acatcccggg gcccagatcg
     3901 ggcaggggct cttcatcgac cacggatcag gagtcgtcat cggggagacc gccgagatcg
     3961 gcgacgatgt caccatctac cagggggtca cccttggagg caccggcaag gagagcggca
     4021 agcggcaccc gacggtggga gacaacgttg tcatcgggac gggcgctaag atcctaggat
     4081 cgctgactat cggcgacaac gccaagatcg gcgcgggcag cgtcgtcgtg cagccggtgc
     4141 cggccaactc cacggtcgtg ggcaatccgg gtcgtccggt gatcacggac ggcaccaggg
     4201 tcggcatccc cgacatcgac tacacgcacc tgcccgaccc cgtggcgcag gcgatgcagt
     4261 gcatcgtgcg gcgtatggtg cagatggaga acgaactcga ggagatctgt cctccgcatc
     4321 agaagcgcaa actcgagcag cgcgaggttc tcgacgagca actctgcaac ctgaccgggt
     4381 actcccaagg atcgggcatc tgacctgcca ctccgggtcg cacctcgcgg atcgcgcatc
     4441 tggcgcgccg tttgacgtcg cggggcgctg cgtgcggtac cattgagagg tgactgaacc
     4501 cgaccgcagc cccgtctccc ccgagccttc cgccgccccc acggctacac gcgcgcccag
     4561 cgcggtctcc gtggtgggcc ccggcgccgc cggcgtcgct cgcgtcggcg aggggctgaa
     4621 cggaccgcag gccgaggcgg tgttccacga agggggcccc ctcctcgtcc ttgcgggggc
     4681 cggctcgggc aagaccaggg tgctcaccca tcgcgtggcg catctcatac agagcgggca
     4741 ggccggcccg tacgagatcc ttgccatcac cttcaccaac aaggctgccg cagagatgcg
     4801 gagccgcgtc gtggagttgg tggggccgga ggctcagaac atgtgggtgt ccacgttcca
     4861 ctccgcgtgt gctcggatct tgcgcaagga ggctcagcta ctcggctacc gttccggcta
     4921 cacgatctac gaccaggacg accaggtgcg actgatgcgt cgctgcctgg aggagttggg
     4981 ccacgacatc aagcgcttcc ccccgcgagc ggtgcacgcg caggtgtcgg acgccaagaa
     5041 ccgcctggtg gacgtggacg agttccagcg gccggggaat ccgggagcgg attcttggga
     5101 ggccggtgta gtcggaaagt cgggcgtcga ctacctcgag gtgaccgcgg cagcgtaccg
     5161 tctctatcag cagcgtctct atgagttgaa cgccatggac ttcgacgacc tcatcatgcg
     5221 gaccgtggac gtgttccgct tgttcgagca tcgtctggcc cactaccgaa acgtctttcg
     5281 tcatatcctg gtggacgagt accaggacac gaaccatgcg cagtatgtgt tggtgaaact
     5341 cctggccgag gagcaccgcc gcatcaccgt cgtgggcgac gacgaccagt cggtctactc
     5401 gtggcgcggc gccgacatac ggaacatcat ggagttcgag gaggatttcg gcgagagcac
     5461 catcatcaag ctcgagcaga actaccgatc gaccacgacg atcctcgacg cggccaacgc
     5521 ggtcgtggcc cacaatcggg gtcggaagcc caagcgcctg tggagtgagc ggggagcggg
     5581 ggagaaggtc ctggtgctgg agtgtcgcga cgaacacgag gaggcgcggc ttgtcgccgg
     5641 agagatccag acgctcctcc gcaaagggcg gcgggccgga gaggtggccg tcttctaccg
     5701 ggtgaacgcc cagtcgcgag tgctcgaaga catcctcgtg cgctacaaca tcccctacca
     5761 ggtcttgggc ggcacgcggt tctacgagcg tgcggagatc aaggacctac tcgcatacct
     5821 gcgggtcgtg gtgaacccct ccgacgacat ctcgttccag cgtatggtca attcgcccaa
     5881 acgcgggttg ggcgcggtag cccaaggcag actgcagctg ttggcggcgg aggacgagac
     5941 ctcgctccgc ggcgcgctgc cgcttgcggc tgacagcgga gacctcgcga ccgccgcccg
     6001 gtccgccttc ggcgagttgg acgggctcat ccgctcgtgg gagcagaggc tgcgcgaagg
     6061 ggcgtcggtg gccgatcagg tgcgggcggt gatggaggag agtgggatgc tcgccgccct
     6121 gcgcgacgcc cgtacgctcg aggccgaggg ccgcctcgag aacctgcagg agttcctggg
     6181 ggtgactcag gagttcgacc gattcaaccc aggcggcggc ctcatcgact tcctcgaaga
     6241 gatcagcctg tatgccgacg tcgactccat gcgtgaccaa gcggaactcg tcaccctcat
     6301 gacactgcac aacgccaaag ggttggagta tcccttcgtc ttcatcacag ggatggagga
     6361 ggggatgttc ccgcacagcc gggccctgga cgagcagaac ctggaggagg agcgcaggct
     6421 ctgctacgta gggatcacgc gcgcgatgga acgagtccac ctttcccatg cccggaaccg
     6481 ttctctgtac gggaccggtg gctaccggat gccgagccgc ttcctcgccg agatacccag
     6541 agagttggtg gaattgcggg gcgccgcccc ggtcgtctcg cgccgtccgg gcgcggggta
     6601 cactccggcc caccggtatc gagcaggagg cggcgccggc cgcggtgc
//
LOCUS       DASRMJ010000114         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_87048, whole genome shotgun sequence.
ACCESSION   DASRMJ010000114 DASRMJ010000000
VERSION     DASRMJ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87048"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2063
                     /locus_tag="VFD74_03255"
     CDS             <1..2063
                     /locus_tag="VFD74_03255"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK48601.1"
                     /translation="TRPSNATVTLANGATISPTTPTGYAGLGIQCEVCHGTGSTANSH
                     MGTGVGVVKFPRILTAEVCGQCHASGTTVETRLGGTSSFSNANGYTTDEVLADYITPY
                     TPANEPTRFYPDGHNKGMNHTYYNEWLLTGHAESRSVLKDEAGDPLPFASEECMRCHS
                     TEGYLKANGYQSSGLTGYTPSPATDETDIECALCHTVHDKTNPSGALGLRVEETEVCS
                     QCHNAEIEEGATIVAGEAVHHPQKEMLEGYGLIGVADMGPSMGSATCVDCHMPETRAG
                     RLSHVFTPMLPGNAEAWGVPELGDSCTPCHESTTRSTLQGNIDGWQAEVDALIANIDA
                     VIAAAPLAGSSIPEFNGAVIDAAVANAEYVTADASGGVHNIRYARAGLTKALYYARAA
                     GSTIDLTGPATADAGSLISLTGTLEFGDGGVGAAQTIKIMKKAAGSDTAVKVATVVTN
                     GSGVFTAQIHPGSSGEYTAVWTPLEGADLTSDVHALTTSVGAALFADVPSTHIFFNYI
                     QGLAKFGVVQGFSPGIFGPDAPVLRAQAAKMLVNALGFGSTTWTNWGHPTFVDVPQPA
                     AQTEANRFPFDFVEKAYVNDIVKGVTATTFVPYGNITRWQLALMIARAGGSHLPPMTG
                     SVMPFTDLAGVSNEVQSAILLCHDYGILDGTSATTFDPWSSATRGQAAKMIWNLAMLT
                     GDLQ"
     gene            complement(2131..>2968)
                     /locus_tag="VFD74_03260"
     CDS             complement(2131..>2968)
                     /locus_tag="VFD74_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HZK48602.1"
                     /translation="KQKLYLQDSYRSEFEARVDAAAPGRVALSATAFFPGGGGQPPDV
                     GELEIAGVWYRVTGICPDGEGRVWHVIEGAAGRGGAGRAAVPAAAGPGGPVAAAVPDA
                     PPEDAGLFGGVPSVGAVARGRIDWPRRHAFMRHHTLLHIVNSVVLADHGGLITGVQIA
                     EDKSRIDFKLDGLDSELVAALEARVNGVITRDLVVSAGLVTEAEFRLRPELVRTAGVE
                     PPVVDGWVRVVSIEGFDAQACGGTHVRRTGEIGACTVLRTENKGKNNKRLYLAVQDPC
                     TG"
BASE COUNT          545 a         1056 c          931 g          436 t
ORIGIN      
        1 tgacgcggcc ctcgaacgcg acagtcaccc tcgccaacgg cgcgacgatc tcccctacca
       61 cgccgaccgg ctacgccggc ctcggcatcc agtgtgaggt ctgccacggc accggctcta
      121 cggcgaacag ccacatgggc accggcgtgg gtgtggtcaa gttccctcgc atcctcacgg
      181 cggaagtgtg cggacagtgc cacgcctctg gaacgaccgt ggagacgcgc ctgggcggca
      241 cgagctcgtt cagcaacgcg aacggctaca ccaccgacga ggtgctggcc gattacatca
      301 ccccctacac gccggcgaac gagcccaccc gcttctaccc cgatggccac aacaagggca
      361 tgaaccacac ctactacaac gagtggctgc tcaccgggca cgccgaatcg cgttccgttc
      421 tcaaggacga agccggtgac cccctgcctt tcgccagtga agagtgcatg cgttgccact
      481 ccaccgaggg ctatctcaag gcgaacgggt accagagcag cgggctgacg ggctacacgc
      541 ccagcccggc gaccgacgag accgacatcg agtgtgcact ctgccacacc gtccacgaca
      601 agaccaaccc cagcggggct ctcggtctgc gcgtggaaga gaccgaagtg tgctctcagt
      661 gccataacgc agagatcgaa gagggcgcca ccatcgtggc cggcgaagcc gtgcaccatc
      721 cgcagaagga gatgctcgaa ggttacgggc tcatcggcgt ggccgacatg ggaccgtcca
      781 tgggcagcgc cacctgcgtc gactgccaca tgcctgagac gcgcgcggga cgcttgagtc
      841 acgtcttcac ccccatgctc ccgggcaacg ctgaagcctg gggcgtgccg gagttgggcg
      901 actcgtgcac accgtgccac gagtccacga ccaggtccac cctacagggg aacatcgacg
      961 gttggcaggc ggaagtagac gctctcatcg cgaacatcga cgcggtgatc gcggctgcgc
     1021 cgctggccgg gtcgtccatt cctgagttca acggagcggt gatcgatgcc gccgtcgcca
     1081 acgccgagta cgtgaccgca gacgccagtg gaggcgttca caacatccgc tatgcccgcg
     1141 ccggcctgac gaaggcactc tactatgcca gagcggcggg cagcaccatc gacctcaccg
     1201 gaccggcaac ggccgatgca ggtagcctga tctcccttac cggcaccctc gagttcgggg
     1261 atggcggcgt cggtgcagct cagaccatca agatcatgaa gaaggcggcc ggatccgaca
     1321 ccgccgtgaa agtggccacc gtcgtgacca acggcagtgg cgtgttcacc gcgcagatcc
     1381 acccgggatc gtccggcgag tacaccgcgg tctggacacc cctcgagggc gccgatctga
     1441 cgagtgacgt gcacgcgctc accacgagcg tcggcgccgc actcttcgcc gacgtcccct
     1501 cgacgcacat cttcttcaac tacatccagg gactggccaa gttcggcgta gtgcagggct
     1561 tctcgcccgg tatcttcggg ccggacgcac cagtcctgcg tgcccaagcg gccaagatgt
     1621 tggtgaacgc cctcggattc ggatccacga cgtggaccaa ctggggccac cccaccttcg
     1681 tcgacgtgcc gcagccggcc gcacagacag aagcgaaccg cttcccgttc gacttcgtgg
     1741 agaaggcata cgtcaacgac atcgtcaaag gagtgaccgc gacgaccttc gtgccgtacg
     1801 ggaacatcac gcgctggcag ctcgcgctca tgatcgcgcg ggcgggtggc agtcacctcc
     1861 cgccgatgac gggttcggtg atgccgttca ccgacctcgc cggcgtcagc aatgaggtgc
     1921 agagtgcgat cctactgtgc cacgactacg gcatcctcga cggcaccagc gccacgacct
     1981 tcgacccctg gtcctcagcc acacgcgggc aggcggcgaa gatgatctgg aacctggcga
     2041 tgctgacggg cgacctgcag tagccgcccg cccagtccgg ttctgagaga cacgacgaac
     2101 ctgaggcccc gggggtcgcc ccccggggcc tcagcccgtg caggggtcct gcaccgccag
     2161 gtacaggcgc ttgttgttct tgcctttgtt ctccgtccgc aggacggtgc acgctccaat
     2221 ctcgccggtg cggcgcacat gagtgccccc gcaggcttgg gcgtcgaagc cctcgatgga
     2281 gacgacccgc acccaaccgt ccacgacagg gggctcaacg ccggccgtgc gcaccaactc
     2341 ggggcgcaaa cggaactcgg cctcggtgac gaggcctgcg ctcaccacca gatcccgcgt
     2401 gataaccccg ttgacgcggg cttcgagtgc cgcgaccagt tcgctgtcga gaccgtcgag
     2461 cttgaagtcg atgcgcgact tgtcttccgc gatctgcacc ccggtgatga gtcctccgtg
     2521 gtcggcaagg accacggagt tcactatgtg cagcagggtg tggtgacgca tgaaggcatg
     2581 gcggcgcggc caatcgatgc ggccgcgcgc cacggcgccc acggatggca caccgccgaa
     2641 gaggccggcg tcttcgggcg gcgcgtcggg gacggcggcg gcgaccgggc cgccggggcc
     2701 cgcggccgcc ggaaccgcag cccgtccggc gccgccccgg ccggcggcac cctcaataac
     2761 gtgccacacg cgaccctcgc catcgggaca gatccccgtg acccgatacc acacaccggc
     2821 gatctccaac tcgcccacgt cgggcggctg ccctcccccg cccgggaaga aggctgtcgc
     2881 cgaaagcgcc actcgacccg gggccgccgc atccacgcgt gcctcgaact cgctgcgata
     2941 tgagtcttgc agatagagct tctgcttt
//
LOCUS       DASRMJ010000115         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_448714, whole genome shotgun sequence.
ACCESSION   DASRMJ010000115 DASRMJ010000000
VERSION     DASRMJ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448714"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFD74_03265"
     CDS             complement(<1..362)
                     /locus_tag="VFD74_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48603.1"
                     /translation="MLRNRLIIPAALGLVVLLVAVVLIQSGGGGRSSTTTTVSTAAVG
                     ASPYDLIEAPGDFEFTTLGEAKFASILLETPEGLTSYMVAADQAAFEAIARSVAAARA
                     VDEPAPDSTSTLTFVMPD"
     gene            665..>2585
                     /gene="ileS"
                     /locus_tag="VFD74_03270"
     CDS             665..>2585
                     /gene="ileS"
                     /locus_tag="VFD74_03270"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018890359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZK48604.1"
                     /translation="MSDRMGDEKTATSVASGLGTASARASSHAHGPGCTQDVCRHDAE
                     HVFRPVDTAQRFPALEEAVLTWWRENGIFEKSVELRRGQPEWVFYEGPPTANGKPGSH
                     HVLARIFKDIYPRFRTMRGNFVPRKAGWDCHGLPVELEIERRLGFNHKDEIEEYGIAE
                     FNQLCKESVTEYVDEWKRMTERIGFWIDLDDAYWTMNDSYIESEWWILTEFWKQGLLE
                     QDYKVVPYCPRCGTALSSHELAQGYKEVTDPSIIVRFPQLDAEGHQEEGTSLLVWTTT
                     PWTLISNVAAAVGGKIEYARARLGDERFILGADLVNAVLGPEAVVEETFPGSALAGRR
                     YRPPFDFVTPDKPAWRVVLGDFVSTDEGTGIVHIAPAFGADDMMVGRANDLPVVMPVD
                     TDVKFTEDVPPYAGMFVKDADKAIIRDLDDLGLLFSTRPYTHNYPHCWRCGTALIYYA
                     KASWYVRTTQRKDDLLAANEAVNWYPEHIKHGRFGDWLENNIDWSLSRERYWGTPLPI
                     WRCPKEHDHSVSSRAELSELSGRDLSELELHRPYVDDVTFPCPTCGAEAHRVPEVIDA
                     WFDSGSMPVAQWHYPFENQELWEQRFPADFICEAIDQTRGWFYSLIAISTLLYKTSSY
                     KNVVCLGHILDGEGRK"
BASE COUNT          478 a          839 c          840 g          428 t
ORIGIN      
        1 ctatcgggca taacgaaggt gagcgtggaa gtggagtcgg gcgcgggctc gtcgaccgct
       61 cgagcggccg ctacggaccg tgctatggcc tcgaaggcgg cttgatcggc cgcgaccatg
      121 taggaggtga gaccttcggg cgtctcgagc aggatgctag cgaacttggc ctccccaagt
      181 gtcgtgaact cgaagtcgcc cggggcttcg atgaggtcgt agggagaagc cccgacggcg
      241 gcagtcgaca ccgtggtggt tgtcgatgaa cggccgcccc cgccgctctg tatgaggacg
      301 acagcgacga gcaggaccac gagaccgagt gcggccggta ttatgagtcg attgcgtagc
      361 acacacactc cagtcggcgc gcggtcgcgc gccggtcgct cacacttgaa gtctcgctag
      421 gcccgcacta gactacgact tcgaaggcag tgacgggcgc ctcagcgccc gcctcgtagc
      481 ttactccagg cgcgagacgg aaaccaacgg cgactcggta gggtggtacc gcgggagagc
      541 accctgtccc aacagcggca ctccgctggg ggcggggttt cgtgctcttc ggacagatat
      601 cgaacggcgg gagccgcacc gtccgacgga ggacggcggc gggcgacacc cgcggaggag
      661 ccacatgagc gacagaatgg gcgacgagaa gaccgcgacc tcagtcgcct cgggactcgg
      721 caccgcgtcc gcccgcgctt cgagtcacgc gcacggtccg ggatgtacgc aggacgtgtg
      781 tcgtcacgat gccgagcacg tgttccgacc ggtggacacg gcccaacgtt tccccgccct
      841 cgaagaggcg gtcctgacgt ggtggcgcga gaacggcatc ttcgagaaga gcgtggagct
      901 gcgccgaggg cagccggagt gggtcttcta cgaaggtcct cccaccgcca atggcaagcc
      961 gggttcgcat cacgtgctcg cccgcatttt taaagacatc tacccgcggt tccgcaccat
     1021 gcgcggcaac ttcgtcccgc gcaaggcggg ctgggattgt cacggcctgc ccgtggaact
     1081 cgagatcgag cggcgtcttg gcttcaacca caaggacgag atcgaggagt acggcatcgc
     1141 cgaattcaac cagctctgca aggagtcggt gaccgagtac gtggacgagt ggaaacgtat
     1201 gaccgagcgc ataggtttct ggatcgacct cgacgacgcc tactggacca tgaacgacag
     1261 ctacatcgag tcggagtggt ggatcctcac cgagttctgg aagcagggcc tccttgagca
     1321 agactacaag gtggtgccct actgcccgcg gtgcggcacc gccctctctt cgcacgagct
     1381 cgcgcagggc tacaaggagg tcacggaccc ttcgatcatc gtgcgcttcc ctcagctcga
     1441 cgccgagggg caccaggagg agggcaccag cctgttggtg tggaccacca ctccgtggac
     1501 actcatctcc aacgtggcgg ccgccgtggg cgggaagata gaatacgccc gcgcccggct
     1561 cggcgacgag cgtttcatct tgggcgcaga cctggtgaac gcggttctcg gcccggaggc
     1621 cgtcgttgag gagaccttcc ccgggtccgc actggcgggg aggcgctacc gtcccccctt
     1681 cgacttcgtc acgcccgaca agccggcgtg gcgcgtggtg cttggtgatt tcgtgagcac
     1741 agacgagggc accggcatcg tgcacatcgc ccccgctttc ggcgccgacg acatgatggt
     1801 ggggcgcgcg aacgacctgc ctgtggtcat gcccgtggac acggatgtga agttcaccga
     1861 ggacgtgcct ccgtacgcgg gcatgttcgt caaggacgcc gacaaagcca tcatccgcga
     1921 tctggacgac ctgggtctac tgttctctac tcgcccctac acccacaact acccccactg
     1981 ctggcggtgc ggcacggcgc tcatctacta tgccaaagcc tcctggtacg tgcgcaccac
     2041 ccagcgcaaa gacgacttgc tggcagcgaa cgaagcggtg aactggtatc cggagcacat
     2101 caagcacggc cggttcgggg actggctgga gaacaacatc gactggtcgc tgtcacgcga
     2161 gcggtactgg ggcactccgc tgcccatctg gcgctgcccc aaggagcacg accactccgt
     2221 ctcctcgcgc gcggaactct ccgagctctc gggccgcgat ctctcggaac tggagctgca
     2281 ccggccctac gtcgacgacg tcaccttccc ctgcccgacc tgcggtgcgg aagcccaccg
     2341 cgtgcccgaa gtcatcgacg cgtggttcga ctccggctcc atgcccgtgg cacagtggca
     2401 ctatcccttc gagaaccagg agctctggga gcagcgcttc ccggccgact tcatttgcga
     2461 ggccatcgac cagacccgtg gctggttcta cagtcttatc gccatcagca cgctgctcta
     2521 caagacgtcg agctacaaga acgtggtgtg cttgggccac atcctggacg gcgagggtcg
     2581 caaga
//
LOCUS       DASRMJ010000116        21128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_227975, whole genome shotgun sequence.
ACCESSION   DASRMJ010000116 DASRMJ010000000
VERSION     DASRMJ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 21128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21128
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227975"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VFD74_03275"
     CDS             complement(<1..1514)
                     /locus_tag="VFD74_03275"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZK48605.1"
                     /translation="MAATSKAQIETVQIPVEGMTCASCVARVQKGLERVEGVAEASVN
                     FATEKATVSYDPAVVGVDRIVATVKDVGYGVQSEKLTLDIGGMTCASCVSRVEKALAN
                     TPGVLNASVNFGTEAATVEFLTGVVSLSDLRRAVEEAGYTIRELEAGAGEDGEGGPDH
                     VEVARARELRTLKLKFGFSLFVGALLMVLSFFPPSFLGRRELWLVMFVLATPIQFWAG
                     LQFYRGAWASAKHRTTDMNTLIAVGTSIAYLYSAAVTFFSGFFETLTGAGFETQVYYD
                     TAAVIIGLILLGRFLEARAKGQTAGAIKKLMGLQAKTARVVRDGAEVDIPIEQVHADD
                     VVIVRPGEKIPVDGVVLEGRSAVDESMLTGESMPVEKGPGDEVIGATLNKTGSFRFRT
                     TKVGKDTALAQIIRLVEEAQGSKAPIQRLADYIASIFVPVVFAIAAVTFVVWLVFGPA
                     PAFTLAMLAAVAVLIIACPCALGLATPTAIMVGTGKGAENGILIRSGEALERAY"
     gene            1772..1978
                     /locus_tag="VFD74_03280"
     CDS             1772..1978
                     /locus_tag="VFD74_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48606.1"
                     /translation="MTLGQFRLAAVIVTLVALAAFVAFAQAGLRRARFRGRQVQQTTW
                     ISLAATAFVVTCLAALTFVGLGFR"
     gene            complement(2130..3248)
                     /locus_tag="VFD74_03285"
     CDS             complement(2130..3248)
                     /locus_tag="VFD74_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK48607.1"
                     /translation="MTGADALEGWAINIEEVDNLVELGRTRGYVTPEDVAEVLVEVDL
                     STEQIEAIYTRFLELGIEVADEGEGDSDAESETADGPSTPVWKAPEPGHEADLSTTDP
                     VRMYLKEIGRVSLLTAEQEVWLAKRIERGDIQAKHELTEANLRLVVSIAKRYHTDGMS
                     FLDLIQEGNLGLIRAVEKFDYRKGHKFSTYATWWIRQAITRAIADKARTIRIPVHMVE
                     KINKLIRVQRQLLQDRGREPTPDEMAAEMSMSVVEVREILKISQTTVSLETPIGEEED
                     SELGDFIEDEEAIRSFDAVAAQLDKDTVNDALAALPFRERKVIELRFGLKGEHPRTLE
                     EVARRFGVTRERIRQIEAKALSKLQATERARRLKTQLD"
     gene            complement(3281..3700)
                     /locus_tag="VFD74_03290"
     CDS             complement(3281..3700)
                     /locus_tag="VFD74_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK48608.1"
                     /translation="MLKTADIISGKWTLLVLRDLAQGINRFSALERSLQGISPKTLSE
                     RLKALEKAGVITRKSYAEVPPRVEYSLTARGLDLIPLIDHMRDYGSKWLMEASNGSGG
                     PSADCCGNGSGGTSVDCCGDGTSGASAACCGNGSSGS"
     gene            complement(3948..5462)
                     /gene="gatB"
                     /locus_tag="VFD74_03295"
     CDS             complement(3948..5462)
                     /gene="gatB"
                     /locus_tag="VFD74_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZK48609.1"
                     /translation="MTADVSRSAGQGRAVSAPPDSQGWEIVIGLEIHVQLSTRTKMFC
                     GCPVTFGEPPNTQVCPICLGHPGTLPVTNERAVEYAMRIALALDCSITRETIFHRKNY
                     FYPDLPKGYQISQFDLPLGVVGHLDAELEDGSGFSVGITRVHMEEDAGKLVHSGGATG
                     RIQGADYSLADFNRGGTPLVEIVTEPDIRTPEQARAFLVELKSLIEHLDVSDVNMEEG
                     SLRCDANVSIRRPGEALGVKTELKNMNSFRFLQRGLEAEIERQVAALEDGETIHQATV
                     HFDPTTGRVSTLRSKEEAHDYRYFPEPDLVPIVLDEDRIERVRASLPELPAARRARVA
                     DAYGLSPYDAAVLCANRVLGDYFERVAALTGDGKLSANWVMGELSAYLNAGGLPVSES
                     AVTPERLAGLLALLNDGTLSGKLAKEVFQIMVETGADAGAVVAERGMGQISDAGELEA
                     LVGAIVAANPGPAAEFRQGHDRVLGFFVGQVMKQTKGQANPKLTNELLRKHLAG"
     gene            complement(5459..6919)
                     /gene="gatA"
                     /locus_tag="VFD74_03300"
     CDS             complement(5459..6919)
                     /gene="gatA"
                     /locus_tag="VFD74_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZK48610.1"
                     /translation="MDALPLTANGCRDLLAAGEVSATELATLYLDRIESVDDAVGSFL
                     LVDRKGALAQAAAVDAKPAGERLPWEGVPIALKDLLCVKGLETTCASRILKGYRPPYD
                     ATVVAKLRAAGLVFLGKLNMDEFAMGSSTENSGYHVTRNPWDLERVPGGSSGGSAACV
                     AAAEAPWTLGSDTGGSIRQPAALCGVVGLKPTYGAVSRYGLIAFASSLDQIGPLTRDV
                     RDSAALLSLIAGRDPMDSTSLGLPAPISLPDRTDLAGLRFGIIGEYLGESCEPGVREV
                     FDHAVAAIHALGGVCEEVSLPSVEKGLAAYYIIAPAEASSNLARFDGARYGRRAADAD
                     DLLEMYNRTRSEGFGEEVKRRIMIGTYVLSAGYYDAYYGQAQRTRTLIIDDFRRAFEN
                     VDFLISPTSPTVAFKIGERTEDPLSMYLSDLCTIPVNLAGLPGISIPAGHSGGLPVGL
                     QIIGPHFSEQGLLDASYALEQRLAVRTSPPPVEVTR"
     gene            complement(6929..7210)
                     /gene="gatC"
                     /locus_tag="VFD74_03305"
     CDS             complement(6929..7210)
                     /gene="gatC"
                     /locus_tag="VFD74_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZK48611.1"
                     /translation="MIRREEVEHIARLARLTFDEAELGRFQHDLSQIIDYVQTLAELD
                     LTGLAPTAHAVEVKNVLRADEPRPCLSQKEATANGPLVEEGQFVVPRIG"
     gene            complement(7549..7749)
                     /locus_tag="VFD74_03310"
     CDS             complement(7549..7749)
                     /locus_tag="VFD74_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZK48612.1"
                     /translation="MAEGAVKWFNAEKGYGFIARDDGEDVFVHFSEVKVEGFRTLAEG
                     QRVSFEVTQGAKGLQASNVVPL"
     gene            complement(7899..8957)
                     /gene="rpoD"
                     /locus_tag="VFD74_03315"
     CDS             complement(7899..8957)
                     /gene="rpoD"
                     /locus_tag="VFD74_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZK48613.1"
                     /translation="MRELVARGRRAGSLTPEEINEALHDLEVSPEEVEGLYSRLADAG
                     VDVIDDGNGHHSPLEDDYSEEEAFLTKLDLSIDHSAGDPVRLYLKEIGKVHLLTAAEE
                     ISLARRVQARDVDAKRRLTEANLRLVVSIAKRYVGRGLLFLDLIQEGNLGLIRAVEKF
                     DPERGFKFSTYATWWIRQAITRAIADQARTIRVPVHMVETINRLKAMQRMLLQRNGRE
                     PSDSELAAEMGVSAARVRDIMKAAQEPISLATPLGEEDDSELGDFVEDTEADEPLDAV
                     VRKMRCEELLRVLDTLSVRERKVIELRFGLRGEQPRTLEEVGDRFGVTRERIRQVEAK
                     TLNRLKNYREAQGLRELL"
     gene            complement(8996..10810)
                     /gene="dnaG"
                     /locus_tag="VFD74_03320"
     CDS             complement(8996..10810)
                     /gene="dnaG"
                     /locus_tag="VFD74_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZK48614.1"
                     /translation="MSPFIKDASVEAVLAAADIVDVVSGYTSLRKRGATHTGLCPFHQ
                     EKTPSFSVSADKGLYYCFGCGEGGNVFTFLQRLENLSFVEVVEQLAERFSVPIEFDDR
                     GGAERDHSGDKNLFTLLERTTSFYSRFLWESREGETARSYLEGRGLGRGICEEFRVGL
                     APMGWRGLYERVLKDGFTEGELEAAGLLVRQPGRVYDRFRGRLMFPLVDHRGRVVGFG
                     GRTLGEENPKYLNSPEGPVYRKGHLLYGLYQARRAVADADSVLIVEGYTDVLALAQAN
                     VRNAVASMGTALTDNQIGLLSRFTRNVDFMFDADRAGTEAVLRSGEMARARSLHAMVV
                     PPPPGQDPADVAREGGEPAVRRLLANRVSFLRFELTRALDAADTASAAGRVRAFEVVR
                     EILSRASSPKELEEEVRTVADRLRLSPENVDLLLRAEPESAAGDPTGGPRTGSRSGRV
                     VPAYRDRVRSPEVTVEREFLLAVLSNPEEAEPILEAVTPEYFGDPLHREAFVGLKEAL
                     LDREPGEAIRRLVRQDSDLGRLFVRLALESESGHYGAAFLNERHLRLEEQHVGRTLRD
                     LRGRLEAEGGGADIEARLVRLQHLQHEVRAMLANVDEG"
     gene            complement(10873..11988)
                     /locus_tag="VFD74_03325"
     CDS             complement(10873..11988)
                     /locus_tag="VFD74_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZK48615.1"
                     /translation="MNKREIPGRVRLDIEALEAGLSPLAQRASCSRGRALDEEPCTLR
                     TCFQRDRDRIVHCKAFRRLKHKTQVFISPEGDHYRTRLTHALEVSGIARAVARAIRLN
                     EDLTEAVALGHDLGHTPFGHAGEEALDSVLRELGQDGFRHNEQSLRVVDVLENEGRGL
                     NLTWEVRDGILCHTGSQEPATQEGRIVRICDRVAYINHDIDDAVRAGLITPPDLPRDA
                     VALLGASGSERIDTLVHDMVDESDRVGHVTQSAEVRESMEVLRSFLFEHVYPRSLEQS
                     GGVAIHDLLANLFKHYLDHPGLLPGASASVSAGDHLAAEAPIAAEDHLDAAADSIAAD
                     VDRDRLVRMAADHVAGMTDRFAQRAYFEVFVPRSWTL"
     gene            complement(12000..14642)
                     /gene="ppdK"
                     /locus_tag="VFD74_03330"
     CDS             complement(12000..14642)
                     /gene="ppdK"
                     /locus_tag="VFD74_03330"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZK48616.1"
                     /translation="MNVTKYVYDFSEGRADMKSLLGGKGANLAEMTGIGIPVPPGFIV
                     TTQACIEYEANGFYPAGLSEEIEQHLAALEEQTDKQFGDPSDPLLVSVRSGAVFSMPG
                     MMDTVLNLGLNDATAAGLAIATGNPRFAYDSYRRFIQMFGDVVLGVDPQLFEDALTEA
                     KASVRAAQDTDLNADDLVALTERFKAILDEHTGSGFPDDPRAQLHLAIQAVFRSWGNH
                     RARVYRKINGISDSLGTAVNVQSMVFGNKGDTSGTGVAFTRDPSTGENVFYGEFLMNA
                     QGEDVVAGVRHPHPLAMMRDVAPETLDQLYAIRQTLEARYKDMQDIEFTIEDGRLYIL
                     QTRSGKRTAAAALRTAVEMVGEGLITKEIALARIDPGALDQLMHPRLDPSASFNVLAQ
                     GLNASPGAAVGQVVFDADTAEERGERGDPVILVRWETNPDDIHGLAKAQGVITSHGGM
                     TSHAAVVARGMGKPCIAGASELKIDIAARRFTVGDTVVCEGDWITMDGGSGRIMLGTV
                     PLTPPQIDRNFEIILAWADEVRCLAVRTNADTPEDAVRAREFGAQGIGLCRTEHMFMQ
                     EERLPHVRAMIMADSLEEREVHLADLLPFQRDDFDGIFRAMVGLPVTIRLLDPPLHEF
                     LPDYTGLMVELERMRHTGATAAEIELKEAVAAKVKVLHEMNPMLGTRGCRLGIQFPEI
                     YAMQVRAIMEAACDVAADLGQAPIVEIMIPLVGFARELEVMRALTVETASTVLAERGV
                     SVEYKVGTMIELPRAAVTADEIAAHADFFSFGTNDLTQTTLGFSRDDAEGKFLTHYLQ
                     TDLLKDNPFEKLDQSGVGELVRMAAEKSRGVKPGIKLGICGEHGGEPSSVKFCHRVGL
                     DYVSCSPYRVPIARLAAAQAVLEE"
     gene            complement(14951..17038)
                     /gene="glyS"
                     /locus_tag="VFD74_03335"
     CDS             complement(14951..17038)
                     /gene="glyS"
                     /locus_tag="VFD74_03335"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZK48617.1"
                     /translation="MARDFLIEIGTEELPASACGAVLRLLPERVAGLFAADDIDLETG
                     SLRVMVSPRRIAVLITAVPEIQTPRENVQRGPAVEAAFGPDGAPTKAAEGFARAKGVA
                     VGDLQVREENGRGYVFAVSRSEGRATADLLPDICVKIVRDMYFPKNMRWGSRELRFSR
                     PMRSLAALFGDDIVPFEVAGVVSGRASCGHRWLGSAIGLSTPAAYVEALRSVRVMVDH
                     EEREAFLRSELDRLAGERGLTWADPMGKMNEVLYLVEWPTVLEGSFGDEHLRLPADVL
                     VTAMQSHQRYFPLIDPSGALACTFLYVSNGDPAFAPQITAGNERVLEGRIEDAEFSFD
                     KDLASGLEHMAGELDRVVFHVKIGTMADKCDRLVTLVAALAAAVGAPEDSKTHALEAA
                     RLAKADQVSVMVREFADLEGVMGETYALIEGFPGEVAQAIREQYLPDAAGGVAPRTVA
                     GALLATAEKVDNIVGAFACGEPPSGSKDPYGLRRAAMGMVTIAFLHGFEYDVRALVRT
                     AYAQLERFPQIIGADQVVPDAVDFIKERLAKSLTDSGVARDAVEAVLPTSDGFADLRS
                     RAVALESFRGSDGWDDLVVTFTRPSNLARKLPAEASGVAVDPDLFVEDAERGLHEAWV
                     DVDARCVELTAAGDHLSALGVLASLRAPLDRFFDVVLVMAEDEAVRLNRLRLLEGIAA
                     SVGRVARLDRLQG"
     gene            complement(17040..18047)
                     /locus_tag="VFD74_03340"
     CDS             complement(17040..18047)
                     /locus_tag="VFD74_03340"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZK48618.1"
                     /translation="MDKPTFQDVLLGLESFWSAHGCVIWQPYHTEVGAGTFNPATFLR
                     SLGPDPWNVAYVEPSIRPTDGRYGENPYRLGHYYQYQVLLKPAPDNIQDLYLASLEHL
                     GVKLAEHDVRFVEDDWESPTLGAWGLGWEVWIDGMEATQFTYFQQVGGIDLDPISVEL
                     TYGTERLAMYLQGVNSAFDLVWVPGVTYGDVHRVNEAQWSAYNFDVADVGILQRHFTD
                     YEQECESCLARGLVMPAYDFVLKCSHMFNLLDARGAISVTERTGYIGRVRNIARRVAE
                     LYVAQLEERRAAAAESAGASDAPTAGAAASTPAPSDAPAAAPAGSPTAADGVSSDGGE
                     A"
     gene            18305..19624
                     /locus_tag="VFD74_03345"
     CDS             18305..19624
                     /locus_tag="VFD74_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZK48619.1"
                     /translation="MDPVLLSRIQFAFTIGFHFLFVPLSLGLSLAIVAAERRYRRSGS
                     PSDRAASDFWIRLFTSTFAIGVATGITMEFSFGTNWAGYSRFVGDVFGAPLAAEALFS
                     FFLESTFLGVLLFGRGRVSGRFYYAATWLVALGAHLSALWIVIANSWQQTPAGYEVVG
                     GRAVLTDFFAAMMNPSTLPRFSHAVVATWVAGAFFVAGVCAYYLRRGRHREFALAALK
                     PALAFALVASLAMPVIGHWHAGQVWKTQPAKLAAFEGRYESGPNAGMSLFGWVDEDRK
                     RLRFALVAPDVLSFALGGSADTVVAGLDSFPAADRPPVQFTFQTYHLMVALGLYFIAV
                     AALGLYLALRGRLERQPFFLRVLAWSIPLPLLANEVGWAAAEVGRQPWIVYGELRTSD
                     AVSVVVPASHIALSLGLFAAVYALLFAAWLRVVVGIIRRGPAVVEEA"
     gene            19629..20660
                     /gene="cydB"
                     /locus_tag="VFD74_03350"
     CDS             19629..20660
                     /gene="cydB"
                     /locus_tag="VFD74_03350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZK48620.1"
                     /translation="MDLQLTWFVLLAVLVTGYVVLDGFDLGVGILYPFLPRGDEERTA
                     LRHSIGPFWDGNEVWLLTAAGALFAAFPPVYATVFSGFYLPLIAAVLGLIFRAVSIEY
                     RSRDPKWGRMWDLAFFGGSLVPPVVFGVAAGNLVRGLPLDATGDSTASLGFLFNPFAL
                     LVGGLTVSLFVTHGASWLVLRTQGTLRERARRTASAAHWVTAAAIVAATAGAAIAAPE
                     HVGHVLAHWPGWVAVGGVIAGLVAARVGMHRGRAGWSFAGSAVTLASLVAVWAAGSFP
                     VMVPGWAGSPSLSARDSSSSHLALSAMLVIAAIGVPVVLAYTALIYRTFTRSRVGGDG
                     ADGTEDSAY"
     gene            complement(20700..>21128)
                     /locus_tag="VFD74_03355"
     CDS             complement(20700..>21128)
                     /locus_tag="VFD74_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK48621.1"
                     /translation="GGEEPDEREQSDGVLTGHGTILLAEDNREVRALVRNVLRRAGYE
                     VLSAADGEEAFRLGTEYPGPLDLLLTDLVMPGLRGEELYRRLSERRPGLRVLLMSGYP
                     ADDVVKGEIESGAVPFLAKPFTRDQLLRKVREVIESPREP"
BASE COUNT         3365 a         6999 c         7097 g         3667 t
ORIGIN      
        1 ttgtaggcgc gctccagcgc ttcgccggag cggatgagga tgccgttctc ggcccccttg
       61 cccgtcccca ccatgatggc cgtaggggtg gccagcccaa gcgcgcacgg gcaggcgatg
      121 atcagcacgg cgaccgccgc caacatggcc agggtgaagg ccggcgcggg gccgaagacg
      181 agccagacca cgaacgtcac ggcggcgata gcgaagacca ccggcacgaa gatgctcgcg
      241 atgtaatcgg ccagccgctg gatgggagcc ttggaaccct gagcctcctc caccaaacgg
      301 atgatctgcg cgagagccgt gtccttcccc accttggtgg tgcgaaagcg aaacgagccg
      361 gtcttgttca gcgtcgcgcc gatgacctcg tcccccgggc ccttttccac cggcatggac
      421 tctccggtca acatggactc gtcgaccgcc gagcgacctt cgagcaccac tccgtccacc
      481 gggatcttct ctcccggccg cacgatgacc acgtcatcgg catgcacctg ctcgatggga
      541 atgtccactt cggcgccgtc acgcacgacg cgggccgtct tcgcctgcag gcccatcagc
      601 ttcttgatgg caccggcggt ctgccccttg gcccgcgcct caaggaagcg accaagcaag
      661 atcagtccaa tgatgacggc cgccgtatcg tagtagacct gcgtctcgaa cccggcgcct
      721 gtgagcgtct cgaagaaccc ggagaagaag gtgaccgcag ccgagtagag atacgcgatg
      781 ctggtgccca ccgcgatcaa cgtgttcatg tcggtggtgc gatgcttggc ggatgcccag
      841 gcgccgcggt agaactggag gccggcccag aactggatag gcgtcgcgag gacgaacatc
      901 accagccaga gttcacgcct gcccaagaat gaaggcggga agaaactcaa caccataagg
      961 agagcaccca cgaacagact gaagccgaac ttgagcttga gggtgcggag ttcacgggca
     1021 cgcgctacct cgacgtggtc cggccctcct tccccatcct ccccggctcc agcttcgagc
     1081 tcccgaatgg tgtacccggc ttcctccacc gcccgcctca gatcggagag agacacgacc
     1141 cccgtgagga actccacggt ggcggcttcc gtgccgaagt tgacggaggc gttcagcact
     1201 ccgggcgtat tggcaagggc cttctccacc cgtgacacgc aactggcgca ggtcatcccg
     1261 ccgatgtcga gggtgagctt ctccgactgg accccgtagc ccacgtcctt cacggtggcc
     1321 acgatgcgat ccacgccgac cacggcggga tcgtacgaga ccgtggcctt ttcggtggcg
     1381 aagttcaccg aggcctcggc gacgccctcc acacgctcca ggcccttctg cacccgagcc
     1441 acgcacgacg cgcaggtcat gccttccacc gggatctgga ccgtctcgat ctgagccttg
     1501 ctcgttgccg ccatacctac agcccgcctc ttctcgctcg ctcgcccgca agccacggat
     1561 ggatcccacg cgtgggcctc tctatcctac catacccccc tcccctatac cagcagacac
     1621 gatgccgacg cagatcgaag aagtccggct tgttggcaag gctcgatggt atcctgaaga
     1681 gtaagcatca gcgccgctcg cagagcgttg ccggctgcgc cggcattttg cgctggttag
     1741 cctgtccgtc ctcactggca aggaggagca catgactctc ggtcagttcc gtctggcggc
     1801 ggtgatcgtg accctcgttg ctctggcggc cttcgtggcg ttcgcgcagg ccggactacg
     1861 ccgtgcccgg tttcggggac gccaggtaca gcagaccacc tggatttcgt tggcggcaac
     1921 agcgttcgtg gtgacgtgtc tcgccgcctt gacgttcgtg ggcttggggt tcaggtaaga
     1981 ggcggcgtac cctgactcgg acgcccgccc tcctgggaga gcggggtgct aatcaccctc
     2041 ggcagcgggc gcggtgggcg gagactgcgg gtgatgcggg cggcgtcgag ccgcgatgcc
     2101 ggtgggtgat cgccggcggg cgggaccggt cagtccagct gggtcttcag cctgcgcgct
     2161 ctctcggtcg cttggagctt ggagagggcc ttggcctcga tctggcggat ccgctcgcgg
     2221 gtgactccga accgccgggc cacctcttcc agcgtgcgcg gatgctcgcc cttcaagccg
     2281 aacctcagtt cgatgacctt tcgctcccgg aaaggtagag cggccaaggc gtcgttcacg
     2341 gtatccttgt cgagttgcgc ggccacggcg tcgaaggagc ggatcgcctc ttcgtcctcg
     2401 atgaagtccc cgagctcgga gtcctcttcc tccccgatgg gggtctcgag cgagacggtg
     2461 gtctggctga tcttcaggat ctcgcggacc tcgaccaccg acatgctcat ctcggcagcc
     2521 atctcgtcgg gtgtgggctc gcgaccccgg tcttgaagca gctggcgctg cacgcggatc
     2581 agcttgttga tcttctccac catgtgcacg gggatgcgga tggtgcgggc cttgtcggcg
     2641 atagcccggg tgatcgcctg gcggatccac cacgtggcgt aggtggagaa cttgtgcccc
     2701 ttgcgataat cgaacttctc caccgctctg atcaggccca ggttcccctc ctggatcaga
     2761 tcgaggaagc tcatgccatc ggtgtggtag cgcttggcga tggagaccac caagcgcagg
     2821 ttcgcctcgg tgagctcgtg tttggcctgg atatcgccgc gttcgatgcg cttggcgagc
     2881 cagacctcct gctcggccgt gagcagggac acacgaccga tctccttgag atacatgcgc
     2941 acgggatcgg tcgtggacag atccgcctca tgcccgggtt ccggagcctt ccaaacaggg
     3001 gtgctgggtc cgtcggcggt ctcggattcc gcgtccgagt cgccctcgcc ttcgtcagct
     3061 acctcgatgc cgagctcgag gaaacgtgtg tatatggcct cgatctgctc cgtcgagagg
     3121 tcgacctcaa caaggacttc ggcgacatct tcaggcgtca catagccgcg cgtgcgccca
     3181 agctcgacga gattgtcgac ctcctcgatg ttgatagccc agccttccag ggcatccgct
     3241 ccagtcaagg tcgtcgcggc tcaggtcctc ggaggtccgg tcaactgccg ctggatccgt
     3301 tgccgcagca cgcggcggat gctccactgg ttccatcgcc gcagcagtcg acggacgtcc
     3361 cgccggatcc gttgccgcag cagtcggcgg acggcccgcc ggatccgttg ctggcttcca
     3421 tcagccactt cgatccgtag tcccgcatgt ggtcgatgag gggtatgaga tcgagacctc
     3481 tggcggtgag cgagtactcc acccgcggtg gcacctcggc gtagctcttg cgggtgatca
     3541 cacccgcctt ctcgagcgcc ttcaggcgct cagacagggt cttcgggctg atgccctgca
     3601 gcgagcgctc gagagcactg aaacgattga tgccttgcgc caggtcgcgc aatacaagca
     3661 gagtccactt gccggagatg atgtcggcgg tcttcagcac aggacaggta tccttgagga
     3721 tgacagggtg acgcacgatc gtggaccttc cttctgctca gatgtatgtc cgaccctcgg
     3781 atacgggccg caggcgacta ttcaaaagga cagtgcaaag tagcagaatt gggggtcctt
     3841 ttcaaccgcg cggtcggcga gcgggcaatc cgcagccggg tcgggacgag ggccggcgag
     3901 ggccggcgag ggtcgggacg agagccgagg cgccatgccg ggggctgcta cccagccagg
     3961 tgcttcctca ggagttcgtt ggtgagcttg ggattggcct gacccttcgt ctgcttcatc
     4021 acttgcccca cgaagaagcc gagcacgcgg tcgtgcccct gccggaactc cgcagccggg
     4081 cctggattcg ccgcgacgat ggccccgacg agtgcttcca actcgcctgc gtcggagatc
     4141 tggcccatgc cgcgctccgc gacgacggct cccgcgtccg cgcccgtctc caccatgatc
     4201 tggaacacct ctttggccag cttgcccgag agcgtgccgt cgttgagcag cgcgagaagg
     4261 cccgcgagcc tctcgggggt cacggcggac tccgagacgg gcagcccgcc tgcgttgagg
     4321 taggcggaga gttcgcccat cacccagttc gccgagagct tgccgtcgcc ggtgagggcg
     4381 gccacccgct cgaagtagtc gccgagcaca cgattggcgc agaggacggc ggcgtcgtag
     4441 ggggagagac cgtaggcatc ggccacgcgt gcccgccgcg cggcgggcag ctcgggcaac
     4501 gaggcccgca cccgctcgat ccgatcttcg tcgagcacga tgggaacgag gtcgggctcg
     4561 gggaagtagc ggtagtcgtg cgcctcttcc ttgctgcgca gggtggagac gcgaccggtc
     4621 gtcgggtcga aatgcacggt ggcctgatgg atggtctcgc cgtcttccag cgcagcgacc
     4681 tgacgctcga tctccgcctc gaggccgcgc tgcaggaatc ggaaggagtt catgttcttg
     4741 agttccgtct tgaccccgag tgcttcaccc ggccgacgta tggagacgtt ggcgtcgcac
     4801 ctcagcgagc cttcttccat gttgacgtcg gagacgtcga gatgctcgat cagactcttg
     4861 agttccacga gaaaggcccg cgcctgctcg ggggtgcgga tgtcgggctc ggtgacgatc
     4921 tccaccaagg gcgtcccgcc ccggttgaaa tcggcgaggg agtagtcggc tccctggatg
     4981 cgcccggtgg cccctcccga gtggaccagc tttccggcgt cctcctccat gtgcacgcgc
     5041 gtgatgccca cgctgaatcc cgagccgtct tccagctccg cgtctaggtg gccgaccacc
     5101 cccaggggca gatcgaactg ggagatctgg tagcccttgg gcaggtcggg atagaagtag
     5161 ttcttccggt ggaagatggt ctccctcgtg atgctgcaat cgagcgccag ggcgatgcgc
     5221 atggcgtact ccaccgcccg ctcgttcgtg acggggaggg tgccggggtg acccaggcag
     5281 atagggcaca cctgcgtgtt gggaggctct ccgaaggtca ccggacagcc gcagaacatc
     5341 ttggtgcggg tgctcagctg cacgtggatc tcgagaccta tgacgatctc ccatccctgg
     5401 gagtccggcg gcgccgagac ggcccgaccc tggcccgcgc tccgcgagac gtctgcggtc
     5461 atcgggtcac ctccacgggc ggggggcttg ttcgcactgc tagtcgctgc tccaaagcgt
     5521 acgacgcgtc gagcaggcct tgctcgctga agtggggtcc gatgatctgc agccccaccg
     5581 gcagtccgcc cgagtgcccc gccgggatac tgatgcccgg gagcccggcc aggttcaccg
     5641 ggatggtgca gaggtccgag aggtacatgg agagcgggtc ctcggtgcgc tccccgatct
     5701 tgaacgccac cgtgggagag gtgggcgaga tgaggaagtc cacgttctcg aaggcgcggc
     5761 ggaagtcgtc gatgatcagc gtgcgcgtgc gttgcgcctg accgtagtag gcgtcgtagt
     5821 acccggcgga gaggacgtag gtgccgatca tgatgcgccg ctttacctct tcgccgaaac
     5881 cctcgctgcg ggtgcggttg tacatctcga gcaggtcgtc ggcgtcggcc gccctgcgcc
     5941 cgtagcgggc gccgtcgaac cgggccaggt tggagctggc ctcggccggc gcgatgatgt
     6001 agtaggctgc gaggcccttc tcgacactgg ggagactgac ctcttcgcat accccaccca
     6061 gggcgtggat ggcagcaacg gcgtggtcga agacctcacg cacgccgggc tcgcacgact
     6121 cgcccagata ctctccgatg atgccgaacc ggagcccggc gagatcggtg cgatcgggca
     6181 gcgagatggg cgcgggaaga cccaagctcg tagagtccat ggggtcacgc cccgcgatca
     6241 ggctgaggag ggcggcagag tcgcgcacgt cgcgggtgag cggtccgatc tgatcgagtg
     6301 agctggcgaa ggcgatgagc ccgtagcggc tcacggctcc gtaggtgggt ttgagcccga
     6361 ccacgccgca gagcgccgca ggctggcgga tggagccgcc ggtgtcgctg cccagcgtcc
     6421 agggcgcttc ggcggcggcc acgcacgcgg cggagccgcc gctcgatccg ccggggacgc
     6481 gttcgagatc ccaggggttg cgggtgacgt ggtagcccga gttctcggtg gagctgccca
     6541 tggcgaactc gtccatgttg agcttcccca ggaataccag gccggcggcg cgcaacttcg
     6601 ccaccacggt ggcgtcgtag ggcggacgat agcccttcag gatgcgcgag gcgcacgtgg
     6661 tctcgagtcc cttgacgcag agcaggtcct tgagcgcgat cggcacaccc tcccacggaa
     6721 gccgctcacc cgcaggcttg gcatccaccg ccgcggcctg ggcgagagcg cccttgcgat
     6781 cgaccagcag aaaggacccg acggcgtcgt cgacactctc gatgcgatcg aggtagagcg
     6841 tcgccaactc cgtggccgac acttcacccg cggccagcag gtctcggcaa ccgttcgcgg
     6901 tgagagggag ggcatccaca gcggccacct agccgatccg tgggacgacg aactgcccct
     6961 cttcgaccag aggaccgttg gccgtggcct ccttctggga gaggcacggc ctcggctcgt
     7021 cggcgcggag cacgttcttg acctctacgg catgtgcggt gggagccaac ccggtgaggt
     7081 cgagctcggc cagcgtctgc acgtagtcga tgatctgaga aaggtcatgc tggaagcgcc
     7141 cgagttccgc ctcgtcgaac gtgaggcggg ccaagcgggc gatgtgttct acttcttctc
     7201 gacgtatcac gaggtggttc tcctttgatg ccgccgctcc acgcgcatcc aatccggctg
     7261 tgcggcggcg aggcccgacc gaccctgtct tgcggcccgc tcgattatat ctcccccaga
     7321 aaagggagag ggcgccttgg ggagggcgcc cttgaggaga aagggagagc tatggatggg
     7381 attactctct cttgccccta tatcggcagc agcgccgaag actttagccc ttttttcaga
     7441 ttcttttcga gggcggggtg aaccacgaaa aagccccgct gaacgcgggg ctttgccgac
     7501 ttccctctga accgtcacac ccccgaagag gtgcggcgaa agacctgctt acagaggaac
     7561 gacgttgctt gcctgcaggc ccttggcgcc ttgtgtgacc tcgaagctca ctcgctggcc
     7621 ttcggcgaga gtgcggaagc cttcgacttt gacctcggag aagtgtacga acacgtcctc
     7681 tccgtcgtca cgggcgatga agccatagcc cttttcggcg ttgaaccatt tgactgcgcc
     7741 ttctgccaac ctgtcctccg ttcggtcgcg accttcgatg tcgttgcatc ggggtcgcac
     7801 ttgtcatggg cgcccttccc gagcgcaccg ctcaagagta tagcaggcct caccgcgggt
     7861 caagccgcca cccgcgccgg acgcaagacg cgggtgcatc acagcagctc tcgcaggccc
     7921 tgcgcctccc tatagttctt gagtctgttc agggtcttgg cctccacctg ccggatgcgc
     7981 tcgcgtgtca ccccgaaccg atcgccgact tcctccaggg tgcgaggctg ttccccgcgg
     8041 agcccgaaac gaagctcaat gaccttccgc tctcgcacgg acagggtgtc gagtacgcgt
     8101 agaagctctt cgcatcgcat cttgcgcacg accgcgtcga gaggttcgtc ggcttcggtg
     8161 tcctcgacga agtcacccag ctccgagtcg tcctcctcgc ccaaaggcgt ggccagagat
     8221 atgggctctt gcgcggcctt catgatgtct cgcacgcggg cggccgacac gcccatctcg
     8281 gcggcaagtt ccgagtcgga cggctctcgc ccgttccgtt gcaggagcat gcgctgcatg
     8341 gccttgagcc tgttgatggt ctcgaccatg tgcaccggca cccggatggt gcgggcctgg
     8401 tcggctatcg ccctggtgat ggcctggcgg atccaccagg tggcgtaggt ggagaacttg
     8461 aagcctctct cggggtcgaa cttctcgacc gctcggatga ggccgaggtt gccctcctgg
     8521 atgaggtcga ggaagagcaa cccgcgcccc acgtagcgct tggcgatgga gaccaccaag
     8581 cgcaggttcg cctcggtgag ccgtcgcttg gcgtcaacat ctcgcgcctg cactcgcctg
     8641 gcgagcgaga tctcctcggc ggcggtgagc agatggacct tcccgatctc cttcaggtag
     8701 agccggaccg ggtcgccggc ggagtggtcg atcgacagat cgagcttggt gaggaacgcc
     8761 tcctcctcgg agtagtcgtc ctcgaggggg ctgtggtgcc cgttcccatc gtcgatcaca
     8821 tcgacgccgg cgtcggcgag tcgcgagtag aggccttcga cctcttccgg ggagacctcg
     8881 aggtcatgga gcgcttcgtt gatctcctca ggggtgagac tcccggcacg tcttccgcgg
     8941 gctaccagtt cacgcatcgc cctttcgctg agttccgcga cgcctgtcac gtctcctacc
     9001 cttcgtccac attcgccaac atggcgcgca cttcatgctg gagatgttgt agtcgcacaa
     9061 gccgagcctc gatgtccgcg cctccgcctt cagcctcgag ccgtcctcgg aggtcgcgca
     9121 gcgtccgacc cacgtgctgc tcttcgagtc gcaggtgtcg ctcgttgagg aaggccgctc
     9181 cgtagtgtcc cgactccgac tcgagagcca gccgcacgaa gagccggccg agatcggaat
     9241 cctggcgcac caagcgccgt atcgcctcgc cgggctcgcg gtcgaggagc gcctccttca
     9301 aaccgacgaa cgcctcccga tgcagtgggt ctccgaagta ctccggagtg acggcctcca
     9361 gtatcggctc cgcctcctcg gggttggaca gcaccgccag caggaactcc cgctccacgg
     9421 tgacctccgg cgatcggacc cggtcccgat aggcggggac cacgcggccc gaccggctgc
     9481 cggtgcgcgg gcccccggtg ggatcacccg ccgcgctctc cggctcggcg cgcagcagaa
     9541 ggtcgacgtt ctcgggcgag agacgaagac gatcggcgac cgtgcgcacc tcctcctcga
     9601 gctccttcgg tgacgaggcc cgggagagga tctcgcgcac cacttcgaac gcgcgcacgc
     9661 ggcctgccgc gctggcggtg tcggcggcgt caagagcgcg ggtcaactcg aatctcagga
     9721 acgagacccg gttcgccagc agacgcctga ccgccggctc ccccccttcg cgggcgacat
     9781 cggccggatc ctggcctggg ggcggcggca cgaccatcgc gtggagagag cgggcgcggg
     9841 ccatctcccc cgagcgcagc accgcctccg tgccggcccg gtcggcgtcg aacatgaagt
     9901 ccacgttgcg ggtgaagcgc gagagaaggc cgatctgatt gtcggtgagc gcggtgccca
     9961 tagaggcgac cgcgttcctc acgttcgcct gggccagcgc cagcacatcc gtgtagccct
    10021 ccacgatcag cacggagtcc gcgtcggcga cggcgcggcg cgcctggtag agaccataga
    10081 gcaggtgtcc cttgcgatag accggtccct ccggagagtt gaggtacttc gggttctctt
    10141 cacctagggt gcgcccgccg aaacccacca cccgcccgcg atgatccacg agcgggaaca
    10201 tgagcctgcc gcggaagcgg tcgtacacgc ggccgggctg gcgaacgagc agccccgccg
    10261 cctcgagttc gccctcggtg aaaccgtcct tgagcacccg ttcgtagaga ccgcgccatc
    10321 ccatgggagc gagccccacg cggaactcct cgcagatgcc cctgccgagt ccgcgcccct
    10381 cgagatacga gcgggcggtc tccccctcgc gactctccca gaggaagcgg gagtaaaacg
    10441 aggtggtgcg ttcgagcagg gtgaacaggt tcttgtcgcc ggagtggtcg cgctcggcgc
    10501 caccgcgatc gtcgaactcg atgggcacgg agaagcgctc ggccaactgc tccacgacct
    10561 cgacgaacga gaggttctcg agtctctgca ggaaggtgaa gacgttgccg ccctcgccgc
    10621 acccgaagca gtagtagagc cccttgtcgg cgctcaccga gaaggagggg gtcttctcct
    10681 ggtggaaggg gcacaatccg gtgtgggtgg cgccgcgctt gcgcaggctc gtgtagccgg
    10741 acaccacgtc gacgatgtcc gccgccgcaa gaaccgcttc aacgctggcg tccttgataa
    10801 aggggctcac cgggctcctc cgtggaacgg tcacgtcgca tttttgcggg agggaaaggg
    10861 agcgcgagta tatcagagag tccaagaacg cgggacgaag acctcgaagt acgcccgctg
    10921 cgcgaagcga tcggtcatgc ccgcgacatg gtcggcggcc atcctgacca ggcggtcccg
    10981 gtcgacgtcg gcggcgatcg agtcggcggc ggcgtcaagg tggtcttcgg cggcgatcgg
    11041 ggcttcggcc gcgaggtggt cgccggcgga cacgctggcc gatgctccgg gcaggagtcc
    11101 tggatggtcg aggtagtgct tgaagaggtt cgccagaagg tcgtggatcg ccactccccc
    11161 actctgctcc aacgagcgcg gatacacgtg ctcgaacagg aacgagcgca ggacttccat
    11221 tgactcgcgt acctcggcac tctgcgtgac gtggccgacg cgatcgctct catcgaccat
    11281 gtcgtgcacg agtgtgtcga tgcgttcgga gccggacgcc ccaagcaggg ccacggcatc
    11341 gcgggggagg tcgggggggg tgatgaggcc ggctcgaacc gcgtcgtcga tgtcgtggtt
    11401 gatgtaggcg actcgatcgc agatgcgtac gatgcgacct tcctgagtcg cgggctcctg
    11461 cgagcccgtg tggcacagga tcccgtcgcg cacctcccaa gtcaggttga gcccgcgacc
    11521 ttcgttctca agcacatcca cgacccgcag gctctgctcg ttgtgccgga aaccgtcctg
    11581 gccgagctcg cgcagaacgc tgtcgagcgc ctcctcaccg gcgtggccga acggagtgtg
    11641 ccccaggtcg tgtccgaggg ccacggcctc cgtgagatcc tcgttgagcc ggatcgcgcg
    11701 ggccaccgcc cgcgcgatcc cggagacctc gagcgcgtgc gtgagccgag tgcggtagtg
    11761 gtcgccctcg ggcgagatga acacctgggt cttgtgcttg agtcggcgga aggccttgca
    11821 gtggacgatg cggtcacggt cacgctggaa gcacgtgcgc agggtgcacg gctcttcgtc
    11881 gagggcccgc ccacgagaac acgaggcgcg ctgcgcgagc ggcgagagcc cggcctccag
    11941 tgcctcgatg tcgagccgca cacggccggg tatctcgcgt ttgttcatct tcgaggcagc
    12001 tactcctcga gcaccgcctg cgcggccgcc aggcgggcga tcggcacccg gtaggggctg
    12061 cagctgacgt agtctaggcc cacgcgatgg cagaacttca cgctcgaagg ctcaccgccg
    12121 tgctcaccgc agatgcccag cttgatgccg ggcttcaccc cacggctctt ctcggcggcc
    12181 atgcgcacca actcgcccac gccgctctgg tcgagcttct cgaacgggtt gtccttgaga
    12241 agatcggtct gcaggtagtg ggtgaggaac ttgccctcgg cgtcgtcgcg cgagaagccc
    12301 aaggtggtct gggtgaggtc gttggtgccg aagctgaaga agtcggcgtg ggcggcgatc
    12361 tcgtcggcgg tgacggcagc ccgcggcaac tcgatcatgg tgcccacctt gtactccacg
    12421 gagacgccgc gctcggccag cacggtcgac gccgtctcca cggtgagtgc gcgcatgacc
    12481 tcgagttccc tggcaaagcc caccaagggg atcatgatct ccactatcgg cgcctgaccc
    12541 agatcggcgg ccacgtcgca ggcggcttcc atgatcgccc gcacctgcat ggcgtagatc
    12601 tcggggaact ggatgccgag gcggcagccg cgcgtgccga gcatggggtt catctcgtgc
    12661 agcaccttga ctttggcggc gaccgcttcc ttcaactcta tctcggcggc ggtcgcaccc
    12721 gtgtgacgca tgcgttccag ctccaccatg agaccggtgt agtcgggaag gaactcgtgc
    12781 agaggcggat caagcaggcg gatcgtcacc ggcagtccga ccatggcccg gaagatgccg
    12841 tcgaagtcgt cacgctggaa gggcagaaga tcggccaggt gtacctcgcg ctcctccagg
    12901 ctatcggcca tgatcatggc ccggacgtgt ggtagccgct cttcctgcat gaacatgtgc
    12961 tcggtgcggc ataggccgat gccctgcgcg ccgaactctc gagcccgcac cgcgtcctca
    13021 ggcgtgtcgg cgttggtgcg caccgcgagg caccgcacct cgtcggccca ggccaagatg
    13081 atctcgaagt tgcggtcgat ctgaggcgga gtgagcggca ccgtgccgag catgatccgg
    13141 ccgctgccgc catccatggt gatccagtcg ccctcgcaga ccaccgtgtc gccaacggtg
    13201 aagcgccggg cggcgatgtc gatcttcagt tcggacgcac ccgcgatgca cggcttgccc
    13261 atgccccgcg ccaccaccgc agcgtgagac gtcatgccgc cgtggctcgt gatcactccc
    13321 tgggccttgg ccagcccgtg gatgtcgtcg gggttcgtct cccagcggac gaggatgacc
    13381 gggtcgcccc tctcaccccg ctcctcggca gtgtccgcgt cgaacaccac ttggcccacc
    13441 gcagcgcccg gcgaagcatt caggccttgg gccagcacgt tgaacgacgc cgacgggtcg
    13501 aggcgggggt gcatgagttg gtcgagcgct cccgggtcga tgcgcgcaag ggctatctcc
    13561 ttggtgatca agccctcgcc gaccatctcg acggctgtgc gcagagccgc ggctgcggtg
    13621 cgcttgccgc tacgggtctg aagtatgtag aggcgaccgt cctcgatggt gaactcgatg
    13681 tcctgcatgt ccttgtagcg ggcttcgagt gtctggcgga tggcgtagag ttgatcgagg
    13741 gtctctggcg ccacgtcgcg catcatcgcg agcggatgcg ggtgacgcac accggccacg
    13801 acgtcttctc cttgggcgtt catgagaaac tccccgtaga acacgttctc gccggtggag
    13861 ggatcgcggg tgaaggcgac gcctgtcccc gacgtgtcgc ccttgttgcc gaacaccatg
    13921 gactgcacgt tcacggccgt gccgagagag tcggagatgc cgttgatctt gcgatagacg
    13981 cgggcccggt gattgcccca gctgcggaag accgcctgga tggcgagatg aagctgcgca
    14041 cggggatcgt cggggaagcc ggaccccgta tgctcgtcca ggatggcctt gaagcgctcg
    14101 gtgagcgcca cgagatcgtc ggcgttcaga tcggtgtcct gcgccgctct cactgaggct
    14161 ttcgcctcgg tgagagcgtc ctcgaagagt tgcgggtcga cgccgagcac gacatcaccg
    14221 aacatctgga taaagcgccg ataggagtcg taggcgaagc gcgggttgcc ggtggcgatg
    14281 gcaagcccgg ccgcggtggc gtcgttgagt ccgaggttga gcacggtatc catcatgccc
    14341 ggcatggaga acaccgctcc cgaccgcacc gagaccaaca gcgggtcgga gggatcgccg
    14401 aactgtttgt cggtctgctc ctcgagcgcg gcgaggtgtt gctcgatctc ctctgagagg
    14461 ccggccgggt agaatccgtt ggcctcgtac tcgatacaag cttgggtggt gacgatgaaa
    14521 cccggaggca cgggaatgcc gatccccgtc atctcagcca ggttcgcccc tttgcccccc
    14581 aggagggact tcatgtcggc ccgcccctcg gaaaagtcat agacgtactt ggtgacgttc
    14641 atgcggttgg ttctccttca ggccgctcgg acgggcgtca caggatgaaa aggcccccgt
    14701 tggaggcccg cacattgtat atgaggcgcc ggatcttgtg aagccgaagg caaacaggcg
    14761 gagaccccga cgctgaccga cgtacagggg tgccgagaga gaagccgaga gagaggaggg
    14821 ccggatcttc ggcctgagcg tcggccggac gaccggacag agcgccggcc gaccgggtag
    14881 ccggcgcggc ccgtgccggc cgcaccgcgc agtcagcgcg gcaagcgccc gtcagaaccg
    14941 cgcgctcggc ctacccctgg agccggtcca gacgggctac ccggcccacc gaagctgcga
    15001 tcccctccag gagacgcagt cggttgagcc gcacggcctc gtcctccgcc atcaccaaca
    15061 caacgtcgaa gaagcggtcg agcggcgccc gcagcgacgc aagcacgccg agggcgctga
    15121 gatggtcgcc tgctgctgtc agctcgacgc accgcgcgtc gacgtccacc cacgcctcgt
    15181 gcagtccacg ttccgcgtct tccacgaaca gatccgggtc gaccgccacc cccgaggctt
    15241 cggccggtag cttgcgggca aggttcgagg ggcgggtgaa cgtcaccacc agatcgtccc
    15301 agccgtcgga accgcggaaa ctctcgagag ccaccgcccg gctacgcagg tcggcgaagc
    15361 cgtcggatgt ggggagcacg gcttccacgg cgtcacgagc gacaccgcta tctgtgaggg
    15421 acttggcgag gcgttccttg atgaagtcca ccgcgtcggg caccacctga tcggcgccga
    15481 tgatctgagg gaagcgttcg agctgcgcgt aggcggtgcg caccagcgcc cgcacgtcgt
    15541 actcgaagcc atgcaggaag gcgatggtga ccatacccat ggcggcgcgg cgcagtccgt
    15601 aggggtcttt ggatcccgag gggggctcac cgcaggcgaa ggcccccacg atgttgtcga
    15661 ccttctcggc ggtggccagc agggccccgg cgacggtgcg cggggcgact cccccggcgg
    15721 cgtccggcag gtactgctcg cggatggcct gcgccacctc accggggaag ccttcgatca
    15781 gcgcgtaggt ctcgcccatc actccttcga ggtcggcgaa ctcgcgcacc atgaccgaca
    15841 cctggtcggc tttcgccaat cgggcggcct cgagagcgtg ggtcttggag tcctcgggcg
    15901 ccccgaccgc tgcggccagc gcggcgacga gggtcaccag ccggtcgcac ttgtcggcca
    15961 tcgtgcctat cttgacgtgg aacaccacac ggtcgagttc gcccgccatg tgctccaacc
    16021 ccgaggcgag gtccttgtcg aacgagaact cggcatcttc tatcctgcct tcgagcacgc
    16081 gctcgttgcc cgcggtgatc tgcggagcga aggccggatc gccgttcgag acgtagagga
    16141 aggtgcaggc caacgccccc gacgggtcta tcaacgggaa gtaccgctgg tgcgactgca
    16201 tggcggtgac cagcacgtcg gccggcagac gcaggtgctc gtcgccgaag gagccctcga
    16261 gcactgtggg ccactccacc aagtagagca cctcgttcat cttgcccatc ggatcagccc
    16321 aggtgaggcc ccgctcgccc gccagccgat cgagctcgga gcgaaggaag gcctcgcgtt
    16381 cctcgtgatc gaccatgacg cgcacggagc gcagagcctc gacgtatgcc gcaggggtcg
    16441 agagtccgat ggcgctgccc agccagcgat ggccgcacga cgcgcgcccc gacacaacgc
    16501 ccgccacctc gaagggcacg atgtcgtctc cgaacagggc cgccaaggac cgcatcgggc
    16561 gcgagaagcg cagctctcgc gagccccagc gcatgttctt ggggaagtac atgtcgcgca
    16621 cgatcttcac gcagatatcc gggagaaggt cggccgtggc gcgaccttcg gagcgcgaca
    16681 cggcgaacac gtagccgcga ccgttctcct cgcgcacctg cagatcgccc accgccacgc
    16741 ccttcgcgcg ggcgaagccc tccgccgcct tggtgggcgc accgtcgggg ccgaacgccg
    16801 cttcgacggc aggcccccgc tggacgttct cgcggggcgt ctggatctcg ggcactgcgg
    16861 tgatgagcac ggctatgcgc cgcggagaca ccatcactcg gagcgagccg gtctctaggt
    16921 cgatgtcgtc ggcggcgaag aggccggcga cgcgctccgg gaggagccgc aacacggcgc
    16981 cgcacgcgga ggcgggcagc tcttcggtgc cgatctcgat gaggaagtct cttgccatgt
    17041 caggcctccc caccgtcgct cgagactccg tcggcggcgg taggcgagcc ggcaggggcg
    17101 gccgcgggtg catcggaggg ggcgggcgta gaggccgcag cgccggcggt gggtgcgtcg
    17161 gaggcgcccg ccgactcggc cgcggccgcc ctgcgctctt cgagttgggc gacgtagagc
    17221 tcggccacgc ggcgggcgat attgcgcacg cggcctatat agccggtgcg ctcggtcacg
    17281 ctgatggcgc cgcgggcatc caggaggttg aacatgtgcg agcacttgag gacgaagtcg
    17341 taggcaggca tgacgagacc ccgagccagg cagctctcgc actcctgctc gtagtcggtg
    17401 aagtggcgct ggaggatgcc gacgtcggcc acgtcgaaat tgtaggccga ccactgcgcc
    17461 tcgttgaccc ggtgcacgtc gccgtaggtg acgcccggca cccacaccag gtcgaaggcg
    17521 ctgttgacac cttgaaggta catggccagc cgttcggtgc cgtaggtgag ttcgacgctg
    17581 atgggatcga ggtcgatgcc gcccacctgc tggaagtagg tgaactgggt ggcttccatg
    17641 ccgtcgatcc agacctccca gcccaacccc cacgcgccca gggtcggcga ctcccagtcg
    17701 tcttcgacga agcgcacgtc gtgctcggcg agtttgacgc ccagatgctc gagcgaggcc
    17761 agatacaggt cctggatgtt gtcgggagcc ggcttgagca gcacctgata ctggtagtag
    17821 tgccctaggc gatacgggtt ctcgccatag cgtccgtcgg tgggtcggat ggagggctcc
    17881 acgtaggcca cgttccacgg gtcgggtccg agggagcgga ggaaggtggc ggggttgaac
    17941 gtgcccgcgc ccacctctgt gtggtagggc tgccagatca cgcatccgtg ggccgaccag
    18001 aagctctcca ggccgagaag tacatcttga aaggtgggtt tatccatgct cacaatgacc
    18061 tctggacgcg ttggcggtcc ctctgccggg gtggaggcga ttgtagcaga cgggtcccaa
    18121 cgggagtacg aacgggagaa ctgggtaggt gggcagccgg gggcgatgtc caggcgcatc
    18181 catgggacgg tcgcccgctc aggcacacca cacgccggac acgagccggg cgtggccaac
    18241 gggaggtatg ggaggcatga actccatgat gcgcgtgtcg cggaagggtc gtaggtgacc
    18301 tggcgtggac ccggtactcc tgtcacgcat ccagttcgcc ttcaccattg gctttcactt
    18361 cctgttcgtg ccgctcagcc tgggtctgtc cctggcgatc gtcgccgccg aacgccgcta
    18421 ccgccggagc ggaagcccct ccgatagagc tgcttccgat ttctggatac ggctcttcac
    18481 gtctacgttc gccatcggcg tggccaccgg gatcaccatg gagttctcgt tcggcaccaa
    18541 ctgggcgggc tactcgcgct tcgtgggcga cgtgttcgga gctccgctgg ccgccgaggc
    18601 gctcttctcc ttcttcctcg aatcgacatt cctgggcgtg ctcctcttcg gcaggggtcg
    18661 agtatcggga cgcttctact acgccgccac ctggctcgta gcgctggggg cacatctctc
    18721 cgccctgtgg atcgtcatcg ccaactcctg gcagcagact cccgccggct acgaggtcgt
    18781 gggcggcagg gcggtcctca ccgacttctt cgccgcgatg atgaacccgt ccactctccc
    18841 tcgcttcagc cacgccgtcg tggccacctg ggtcgccggc gccttcttcg tggccggcgt
    18901 gtgcgcctac taccttcgcc gcgggcgaca ccgcgagttc gcgctcgccg ctctgaagcc
    18961 cgcgctcgcg ttcgccctgg tggcctctct ggccatgccg gtgatcggcc actggcacgc
    19021 gggccaggtg tggaagaccc agccggccaa gctggccgcg ttcgagggtc gatacgaatc
    19081 cggcccgaac gccggcatgt cgctcttcgg atgggtggat gaagaccgga aacgactccg
    19141 gttcgcgctg gtggctcccg acgtcctcag cttcgcgctc ggcggctccg ccgacaccgt
    19201 ggtggcggga ctcgactcct tcccggcagc cgaccggccg ccggtgcagt tcaccttcca
    19261 gacataccac ctcatggtgg ccctcggact ctacttcatc gcggtcgcgg ccctcggact
    19321 ctacctggcc ctgcgcgggc gactcgaacg ccaaccgttc ttcctgcgcg tgctggcctg
    19381 gtcgatcccc ctgcccctgc tcgccaacga ggtgggctgg gcagccgcgg aggtgggcag
    19441 gcagccgtgg atcgtctacg gggagttgcg cacgagcgac gcggtgtcgg tggtggttcc
    19501 cgcctcccac atcgctctct ccctcggtct gttcgcggcg gtgtacgcgt tgctgttcgc
    19561 ggcctggctc cgggtcgtgg tggggattat ccgacgcgga ccggccgtgg tcgaggaggc
    19621 gtgatctgat ggaccttcaa ctcacctggt tcgtgctgct ggccgtgctg gtgaccgggt
    19681 acgtggtgct cgatgggttc gacctgggag taggcatcct ctacccgttc ctcccccgcg
    19741 gcgacgagga gcggaccgcg ctgcgccact ccatcgggcc cttctgggac ggcaacgagg
    19801 tgtggctgct caccgccgcg ggcgccctgt tcgccgcctt ccctcccgtg tacgctacgg
    19861 tgttcagcgg cttctacctg cccctgatcg cggccgtcct cggactcatc ttccgcgccg
    19921 tgtcgatcga ataccggtcg cgcgacccga agtggggtcg catgtgggat ctcgccttct
    19981 tcggcggcag cctggtgccg cccgtcgtgt tcggagtggc tgcggggaac ctcgtgagag
    20041 gattgccgct ggacgcgacg ggcgactcca cggcgtccct cgggttcttg ttcaatccct
    20101 tcgcgctgct ggtgggcggc ctcaccgtgt cgctgttcgt gacgcacggc gcctcgtggc
    20161 tggtgctgcg cacgcagggg accctgcgcg aacgggcgcg gcgcacggct tccgcggcgc
    20221 actgggtgac ggcggccgca atcgtggccg cgacggcggg ggccgcgatc gcggcccccg
    20281 aacacgtcgg gcacgtcctc gcgcactggc ccggatgggt ggctgtgggt ggagtgatcg
    20341 cagggttggt ggctgcccgc gtcgggatgc accgtggacg cgccgggtgg tcgttcgcgg
    20401 gcagcgcggt cacgttggcg tcactggtgg cggtgtgggc ggcgggctcg ttcccggtga
    20461 tggtcccggg gtgggccgga tcgccgtcgc tgagcgcgcg cgactcctca tcgtcgcacc
    20521 tggccctgag cgcgatgctc gtcatcgccg cgatcggagt gccggtggtc ctggcgtaca
    20581 cggcgctcat ttaccgcaca ttcacgcgga gcagggtcgg cggcgacggc gctgacggaa
    20641 cggaggattc cgcctactag gaggctgcta agaggcgaag ccttggcccc cggtgcccgt
    20701 cagggctccc gcggactctc gatcacttcg cgcaccttcc gtaacagctg atcgcgcgtg
    20761 aagggcttgg cgaggaatgg aacagcgccg ctctcgatct cgcccttcac cacgtcgtcg
    20821 gcgggatagc cggacatgag caacaccctg agaccggggc gccgctcgct caggcgtcgg
    20881 tagagttctt cgccgcgcag gcccggcatc accaggtcgg tgagcaggag gtccagcgga
    20941 cccgggtatt ccgtgccaag tcggaaggcc tcctcaccat cggcggcgct gagcacctcg
    21001 tagcccgcgc ggcggagcac gttccgtacc agagcacgca cttctcggtt atcctcggca
    21061 agtaggatgg tcccatggcc cgtaagaacg ccgtcgctct gctcacgctc gtccggctct
    21121 tccccacc
//
LOCUS       DASRMJ010000117         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_189987, whole genome shotgun sequence.
ACCESSION   DASRMJ010000117 DASRMJ010000000
VERSION     DASRMJ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189987"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VFD74_03360"
     CDS             complement(<1..779)
                     /locus_tag="VFD74_03360"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HZK48622.1"
                     /translation="MTRAEGKPPALEARGLCVRRSDRTLLEDVDLRVEAGAFLGILGP
                     NGSGKTTLLRALTRAMEPSSGEVLLEGRPVSTYDRRAQARKIGVVPQTFTLDFRFTVE
                     EVVALGRYPHRAAWGGSDDHDDAAIPAAMAALSITDLADRPVTELSGGERQRVLIAQT
                     LAQETPILLLDEPLNNLDLNHQLEVMQVLARLNAEGRTVVVVVHDINMAAQYCAELLL
                     LRGGRVAAHGTPDQLLTPETLLEVFSTRVAVHRQGGRPYVTP"
     gene            complement(846..1844)
                     /locus_tag="VFD74_03365"
     CDS             complement(846..1844)
                     /locus_tag="VFD74_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007978551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HZK48623.1"
                     /translation="MAGLAAALFAALLLATAVGAANLSPPQVVRALLSLVAPGSVDSA
                     GYPAWAPGLLLDLRLPRIIMALIAGVALSTAGATFQGVFRNPLAEPYLLGVSAGAAVG
                     ATIAILWKPLAVFGVFGLPALAFVGAITAAFLVYKLATFAGETSGASLLLSGVAVGST
                     FTAVLSLLMVATQYDLRTVILWLMGGLTTASWTKVKVAAPLVAVGVVAMLAHAPRLNL
                     MLMGESRAEELGVNAHATRRNLLVIASLTTAGAVAFTGLIGFVGLMVPHMVRLLLGPD
                     HRYLLPACGMFGALFLLLADTFARTALSPAEIPVGIITAFVGGPFFLYLLRIRKGV"
     gene            complement(1915..2913)
                     /locus_tag="VFD74_03370"
     CDS             complement(1915..2913)
                     /locus_tag="VFD74_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK48624.1"
                     /translation="MHDQTTLGSPMRRRALALFVALLSVTWAVLALSGCSSGATTTTS
                     EATPTTEATATTTPAEDQAAFPVTVTDDNGVEVTVTARPERIVSTAPANTEILFALGA
                     GDRVVGVTSLDDYPPEVADIEKIGDFSPNTEAIIALEPDLVVGYSGNEEALQPLVDAG
                     IPVLILNAASIDGIYTDMTIVGNAIGEPKAAEDLIATVRVELEGIANTAAETGESPKV
                     FYALDDTLWTVGPGSFVDELITLAGATNVAANGPSPYFQYTPEELVAADPDAVILPMT
                     VFSTIDAFAADPRFAALRAVKEGRVYLVDDITVTRPGPRVTEGLTVLAKALHPDAF"
     regulatory      complement(3052..3202)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3334..3510
                     /locus_tag="VFD74_03375"
     CDS             3334..3510
                     /locus_tag="VFD74_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48625.1"
                     /translation="MGIGWLSVIVFPLLAVFFGFALLAALERSWRLAALSFVSMTAAL
                     AAVLLTGHTLLPPR"
     gene            3760..>4774
                     /gene="purM"
                     /locus_tag="VFD74_03380"
     CDS             3760..>4774
                     /gene="purM"
                     /locus_tag="VFD74_03380"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZK48626.1"
                     /translation="MAHKRKEPEHKEPEQEPVSAAGDLCASESSVTAGLTYAAAGVDI
                     DAGDEAVRRIKGLAESTYIPGVLGSLGGFSGLYEMPPGMKQPVLVTATDGVGTKLLVA
                     QEMGKLNTVGIDLVAMSVNDVLTVGASPILFLDYIATGKLDPIEIESVVEGVAHGCRE
                     AGCALIGGETAEMPGLYAPKEFDLAGFCVGLAEKENLVDGSKIAEGDIVIGLASSGLH
                     SNGFSLVRRVLEANQCGLADKFPHFGETIAYMLLHPTRIYVNAVWSLLEKGVPIRGMA
                     HITGGGIGGNLSRVVPEGLCAALHFDNWKIPKVFRAIQHLGGIDEVEMFRTFNMGIGY
                     VIVV"
BASE COUNT          772 a         1516 c         1699 g          787 t
ORIGIN      
        1 ggcgtgacgt agggccgtcc gccctgtctg tgcacggcca ccctggtgga gaagacctcg
       61 agcagggtct cgggggtgag gagttggtcg ggagtgccgt gcgccgcgac ccgtcctccc
      121 cggaggagca ggagttcggc gcagtactgg gcggccatgt tgatgtcgtg cacgaccacg
      181 accacggtgc gcccttccgc gttgagccgg gccaacacct gcatgacctc cagctggtgg
      241 ttgaggtcga ggttgttgag gggttcgtcg aggagcagga tgggcgtctc ctgagccagg
      301 gtctgagcga tgagtacgcg ctggcgctcg ccgccggaga gctccgtgac cgggcggtcg
      361 gccaggtcgg tgatggagag ggccgccatc gcggctggga tcgcggcatc gtcgtggtcg
      421 tcgcttccgc cccaggcggc ccgatgcgga tagcgcccta gcgcgaccac ttcctccacc
      481 gtgaacctga agtcgagggt gaatgtctgc ggaaccacgc cgatcttccg ggcctgtgcg
      541 cggcgatcat acgtcgacac gggcctgccc tcgagcagca cctcgccgga cgatggttcc
      601 atggcccgcg tcaatgcccg aagcagcgtg gtcttgcccg acccgttcgg gcctaggatg
      661 ccgaggaagg ccccggcctc cacgcgcagg tccacgtctt cgagcagcgt ccgatcggag
      721 cggcgcacgc agaggccgcg ggcctcgagg gccgggggct ttccttcggc acgcgtcatg
      781 ggcatgggcg cctgacaggc gccgggctgc ggtggccgca gcggttggtg accacggggg
      841 ccacctcaga cccccttgcg gatccggaga aggtacaaga agaaaggtcc gcccacgaat
      901 gcggtgatga tccccaccgg tatctcagcc ggggacaggg ccgtgcgggc gaaggtgtcc
      961 gccagaagga ggaagagggc gccgaacatg ccgcacgccg gcaggaggta gcgatggtcg
     1021 ggacccagga gcaggcgcac catgtgcggc accatgaggc cgacgaatcc gatcaggccg
     1081 gtgaacgcca ctgctcccgc tgtggtgagc gacgcgatca cgagcaggtt gcggcgggtg
     1141 gcgtgggcgt tcacgccgag ctcttcggct cggctctctc ccatcaacat caggttcaga
     1201 cggggggcgt gggcgagcat cgccaccacc cccaccgcga cgaggggggc tgccaccttc
     1261 actttggtcc aggacgcggt ggtaaggccg cccatcagcc acaggatgac ggtgcgcagg
     1321 tcgtactggg tggccaccat cagcagtgag agcacggccg tgaaggtgga gccgacagcg
     1381 actccagaaa gcaacagtga cgcgcccgag gtctctccgg cgaaggtggc gagcttgtac
     1441 acgaggaagg ccgccgtgat ggctccgacg aacgccagcg ccggcaggcc gaacactccg
     1501 aacacggcca acggtttcca cagtatggcg atggtggcgc cgacggccgc gccggccgac
     1561 actccgagga ggtagggctc ggcgaggggg ttgcggaaga ccccttggaa cgtggctccc
     1621 gccgtggaga gagccacgcc cgcgatcagg gccatgatga tcctgggcaa ccgcagatcg
     1681 agaagcagac cgggcgccca cgccggatag ccggcggagt cgacgcttcc gggggccacc
     1741 aggctgagca gggctcgaac cacctggggc gggctgagat tcgcggctcc gacggcggtg
     1801 gccagcagca gcgctgcaaa cagcgctgct gccagaccgg ccatcaggag ccgataccgg
     1861 ctccgccgag aggcacgagc agtcaacggc ccgcctccga ggtcaacgcg ctggctagaa
     1921 cgcgtccgga tgcagtgctt tcgcgagcac cgtcagaccc tccgtcaccc gggggcccgg
     1981 tcgggtcacg gtgatgtcat cgacgagata cacgcgtcct tccttcacgg cgcgcaaggc
     2041 cgcaaaacgc gggtcggcgg cgaaggcgtc gatggtcgag aacaccgtca tcggcaggat
     2101 caccgcgtcg ggatccgcag ccaccagttc ttcaggggtg tactggaagt aggggctggg
     2161 gccattggcc gcaacgttgg tggcgccggc gagcgtgatg agttcatcca cgaacgagcc
     2221 cggtcccacg gtccagagtg tgtcgtccag cgcgtagaac accttggggc tctcgcccgt
     2281 ctcggccgcc gtgttggcga tcccttcgag ttccacccgc accgtggcga tgaggtcctc
     2341 ggccgccttg ggttctccga tggcgttgcc cacgatggtc atgtcggtgt agatgccgtc
     2401 gatcgaggcg gcgttcagga tcagcacggg gatacccgca tccaccagcg gttgcagggc
     2461 ttcctcgttc ccagagtagc ccaccacgag atcgggctcg agcgcgatga tcgcttcggt
     2521 gttggggctg aaatcgccga tcttctcgat gtcggccacc tcgggcggat aatcgtcgag
     2581 actggtgacg cccacgacgc ggtcgccggc gcccaacgcg aaaaggatct cggtgttggc
     2641 gggcgccgtc gagacgatgc gctcgggacg ggcggtcacc gtgacctcga cgccgttgtc
     2701 gtcggtgacg gtcaccggga aggcggcctg gtcttcggcg ggggtggtgg tggccgtagc
     2761 ctcggtcgtc ggagtcgcct ccgacgtggt ggtggtggct ccgctcgaac aaccactgag
     2821 ggccagaacg gcccaggtga cgctcagcaa cgccacgaag agggcgagtg cgcgtcggcg
     2881 catgggtgat ccaagggtgg tttggtcgtg cattggtcgt gctccttccg gttcgacttg
     2941 acctatcggg gcaagatggc ggagagcgtg acatgctccc cgactgggac gagcccagcc
     3001 gaaccgggac ggcgccgcgc gtgaagcgca acggagactg ccctctccac ggagagctgc
     3061 tcctccttcg gcaggtgttc tggctcccgg tgtgggtgtc tgcacgacac ttcacacacg
     3121 gtaacagtgg cgggaccgca ccggtttctc accggtttcc cctttggcct ttgcaggccg
     3181 cgccgaaggg ttccatctga ccttgggatc gactatacca ccgttgccgc gagaaggcgg
     3241 cgacgggggc cgggttcggc gggcgctcga tcgtctggta tcctgtccgg tcggattgac
     3301 cacggggctc agacacgagg aaggaggtgc gcgatgggga ttggatggct cagtgtcatc
     3361 gtcttccctc tcttggccgt cttcttcggg ttcgcgctcc tcgccgctct cgagcggagc
     3421 tggcgtctgg ctgctctctc gttcgtcagt atgaccgccg ccttggcggc agttctcttg
     3481 accgggcata cgctgctgcc tccgaggtag cggcggcgcg gtcgccggcg gcccgcacgt
     3541 gcgcggcccc acgcgccgac tggtgacgcg ccgggtgagc cggcaggccg cgctcccgcg
     3601 gccccacgcg caggcggcct gaggccgcgg tcgcggtgaa cggtctgcta cgatgatggg
     3661 gattctgatc aggtgggcca ccgcgtgcga tgcagcgccg gcggccattc gatacgacga
     3721 gaacgtcggc ggtgaggccg gcggctgcga ggaggacccg tggcgcacaa gcgcaaggaa
     3781 cccgagcaca aggaacccga gcaagaaccc gtctccgccg ccggcgacct ctgtgcttcg
     3841 gagtcgagcg tcactgccgg cctcacctac gcggccgcgg gggtggacat cgatgccggc
     3901 gacgaggccg ttcgccgcat caaggggctc gcggaatcca cctacatccc cggggtgctg
     3961 ggcagtctcg gcggcttctc gggtctgtat gagatgcccc cgggcatgaa gcagccggtg
     4021 ctggtcaccg ccaccgatgg cgtgggcacc aagctcttgg tggcgcagga gatgggcaag
     4081 ctcaacaccg tgggcatcga tctggtggcc atgtcggtga acgacgtgct caccgtgggc
     4141 gcgagcccca tcttgttcct cgactacatc gcaaccggca agctcgatcc gatcgagatc
     4201 gagagtgtgg tcgaaggcgt cgcccacggt tgccgtgaag cggggtgcgc tctcatcggg
     4261 ggggagaccg ccgagatgcc cggtctctac gcgcccaagg agttcgacct tgccggcttc
     4321 tgcgtgggct tggccgagaa ggagaacctg gtcgacggct ccaagatcgc ggaaggtgac
     4381 atcgtcatcg gccttgcttc cagcggattg cactccaacg gcttctcctt ggtccgtcgc
     4441 gtgctcgagg ccaaccagtg cggcctcgcc gacaagttcc cgcacttcgg tgagacgata
     4501 gcctacatgc ttcttcatcc cacgcgcatc tacgtgaacg cggtgtggtc gctgctggag
     4561 aagggcgtgc cgatccgcgg tatggcccac atcacgggcg gcggcatcgg gggcaacctg
     4621 tccagggtgg ttcccgaggg cctctgcgcc gccctgcatt tcgacaactg gaagatcccc
     4681 aaggtctttc gtgcgataca gcatctcggc ggcatcgacg aggtcgagat gttccgcact
     4741 ttcaacatgg gtatcgggta tgtgatcgtg gtcc
//
LOCUS       DASRMJ010000118         7433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_482125, whole genome shotgun sequence.
ACCESSION   DASRMJ010000118 DASRMJ010000000
VERSION     DASRMJ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482125"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFD74_03385"
     CDS             complement(<1..259)
                     /locus_tag="VFD74_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011323521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48627.1"
                     /translation="MDSSQRKEFGDSYARLAGLGLVVEGYETERAELDVSSGFLRVTT
                     TVVLRGGGHEGRGEDVTYAAEDHDPYPLALPLAGDWTLDGLS"
     gene            complement(297..401)
                     /locus_tag="VFD74_03390"
                     /pseudo
     CDS             complement(297..401)
                     /locus_tag="VFD74_03390"
                     /inference="COORDINATES: protein motif:HMM:NF023295.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
     gene            complement(426..2390)
                     /locus_tag="VFD74_03395"
     CDS             complement(426..2390)
                     /locus_tag="VFD74_03395"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZK48628.1"
                     /translation="MATLLWEPLAERVADANMTRFATFAGKRCGRTFESYAALHRWSV
                     DDIPGFWAAAWDFLGIQASTPYTAVIDDLARFPGANWFLGARLNFAENLLRYRDDRVA
                     FIFVGETAAPVRVTYAELYQTVARVAGALRAAGVKPGDRVAGYLPNLIETAVAMLAAT
                     AVGAVWSSCATDIGPEAAADRLSQIEPKVLFAVDGYRYKNRVFDCISNAAAVTRSIPS
                     IERVVVAHYAGDPAADLSSIRGALRWDEFIADQAGEELFFEQAPFDHPAVVMFSSGTT
                     GKPKCLVQSGGGILINQLKELVLHADLRRDDTIFYVTTASWMMWNWLMSALGTGATVV
                     LFDGNPAYPDLGALWRLIADLKVTVFGTSASYLSLLKAQGMVPKEDYELSALREICQT
                     GSALSPDGFAYVYDAIKADLHFNSLSGGTDINGCFAIGSPTLPVYAGQLQAPGLGMKI
                     ECYDEAGKPLRDQVGELVCEAPAPPMPLYFWNDPGNKRYHAAYFDVYPGVWWHGDYVM
                     FDATTGGITFLGRSDAVLKPSGVRIGTAEIYNQVEKLPEIADSLAIGQDWEGDQRIVL
                     FVKLVDGGELTDDLVKKIARTLRENASPRHVPAKVIAVPDIPYTLNMKKVETAVTNLV
                     HGRPVTNRDALANPDSLDFYERLVPGLKEA"
     gene            complement(2456..3238)
                     /locus_tag="VFD74_03400"
     CDS             complement(2456..3238)
                     /locus_tag="VFD74_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZK48629.1"
                     /translation="MEFQNLKVETADGIATVTIDRPQSLNALNRDVVRELEYALFELE
                     LEAAVKVVIITGAGEKSFVAGADIKEVESMDSVQGHAFARRGQRVMLFIQNMKKPVIA
                     AVNGYALGGGLELALACDFIYASETAKLGLTEVTLGIMPGFGGTQNLPRLIGRNKANE
                     LILTGKMIDAHKACAWGIVNEVFPPEDLLAKTKETAAAIAKNGLVAVSHAKDSIANGL
                     DMAKEDGLRYEASMFAVLFATEDQSEGMGAFVAKRKASFKGQ"
     gene            complement(3283..4128)
                     /locus_tag="VFD74_03405"
     CDS             complement(3283..4128)
                     /locus_tag="VFD74_03405"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HZK48630.1"
                     /translation="MIERIGVLGAGQMGNGIAHVFAQEGYEVVMYDIVPEQLEKALVT
                     IRQNLERQAKKGAIPETTVAETMGRLKVTQDMADLAQSDFAVEAVTEHEPLKLDIFRN
                     LDATVKEGAILASNTSSIPITKIAAVTGRPEKVIGMHFMNPVPVMKLVEVIRGHATSD
                     ETFEVTADMVVKLGKEMAVSQDYPGFIINRVLIPMLNEAVFALYEGIATAEDIDKGMK
                     LGLNQPMGPLALADFIGLDTVLAIANVLYDGFKDPKYRPCPLLVKTVNAGYLGRKTGR
                     GFFTY"
     gene            complement(4169..5344)
                     /locus_tag="VFD74_03410"
     CDS             complement(4169..5344)
                     /locus_tag="VFD74_03410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZK48631.1"
                     /translation="MKDVYVVDALRTPFGSFGGSLADVPAPQLAGAVMKALLEKVDLA
                     PEAVDEVIVGNVLAGGCGQAPARQAMRAAGIPDKAAALTVNKVCGSGLKAIQLGADAL
                     QLGHSEVVIAGGMESMSLAPYILKKARTGYRMGNGEIVDLMICDGLQDPYSGQHMGVI
                     ADDSATRNGFSREAQDEFALRSYRLAQAAVTEGVFRDEIVPFVKTGKKGDVTVDTDEG
                     PFKIDLAKLTQLRPAFKKDGTITAGNASPISDGAAVALLTDEAGLKKFGLTPKARLVA
                     AATESLHPDHFPEAPVGAIEKACAQAGLSIGDIDLFEINEAFAVVAMIAIKSLGLDID
                     KVNVNGGACALGHPIGASGGRLPATVVRELQRRQCRYGLAVLCIGGGEAFAAIFERV"
     gene            5887..7044
                     /locus_tag="VFD74_03415"
     CDS             5887..7044
                     /locus_tag="VFD74_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZK48632.1"
                     /translation="MFLELTAEQRLIQDTAREFAKAELDPIAATLDQEGDPPAFLANL
                     RKLAELGFMGLNVKDEYGGAEAGVVAFSVAITEIARACASTAVTLSVNNMVCEVIQAA
                     GSEAQKKAYIPKICSGEFSAGAFALTETGAGSDPSGVATQAVLDGDQWVLNGSKIFIT
                     SAPYAGVFMVWAVTDRSVPKGKGISCFLVEGGTPGLVIGKEEHKTGQHASATNELLFE
                     DCRIPKDALMGKLNDGFRIAVGELAGGRIGIGSLGLGVGLAAIDYATQYSTERTQFGQ
                     KISNFQAIQWMIADTYTELEAARLLLMNAAFKKEQGKPFAKEASMAKMYATEAANRAC
                     YKAVQMLGGYGYTVDFPVERYARDARITAIYEGTNEIQRTIISREILRSIV"
     gene            7054..7197
                     /locus_tag="VFD74_03420"
     CDS             7054..7197
                     /locus_tag="VFD74_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48633.1"
                     /translation="MPFRGGIGLTDQQAIDAWVAELNARPRHERDALMGASPFPDDSD
                     PAT"
     gene            7274..>7433
                     /locus_tag="VFD74_03425"
     CDS             7274..>7433
                     /locus_tag="VFD74_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZK48634.1"
                     /translation="MKVVVFGATGRTGRLLVEGALARGHDVTAFVRAPNKLGALRDRV
                     RVVQGAELV"
BASE COUNT         1268 a         2441 c         2390 g         1334 t
ORIGIN      
        1 ccgagagccc gtccagcgtc cagtctcccg cgagagggag cgccagtggg tacgggtcgt
       61 ggtcctcggc cgcgtaggtc acgtcttccc cgcgcccctc gtgcccgccg ccgcgcagca
      121 cgacggtcgt ggtcacgcgc aggaacccac tggacacgtc cagctcggcg cgttccgtct
      181 cgtagccttc gactacgagc ccgagccccg cgagcctcgc gtagctgtcg ccgaactcct
      241 tgcgttgtga actatccatg ggcagaccct acccagcgac cacggcgctc aagccgtcag
      301 gccgcccact cctcacacac gacttcagcc tgcgccagca cgaactgtga tgcgtgctcc
      361 tgcttgtcgg gagggtagcc gtacttgcgc agcaagcgct tgaccgcgac gcgcgtggcg
      421 tgcggctacg cctctttcaa gccgggcacc aacctctcgt agaagtccaa ggaatccgga
      481 ttcgccaggg cgtctcggtt ggtgaccggc cggccatgga ccaggttggt gaccgcggtc
      541 tcaaccttct tcatgttgag cgtgtacggg atgtcgggca ccgcgatgac ctttgccggc
      601 acgtgccgcg gcgaggcgtt ctcgcggagc gtccgagcga tcttcttgac gaggtcgtcg
      661 gtcaactccc cgccatcgac gagcttcacg aagaggacga tgcgctggtc gccctcccaa
      721 tcttgaccga tggccaggct gtcggcgatc tcggggagct tctccacctg gttgtagatc
      781 tcggccgtgc cgatgcggac ccccgagggc ttgagcaccg cgtccgagcg cccgaggaag
      841 gtgatgccac cggtggtggc gtcgaacatg acgtagtctc cgtgccacca cacgcccggg
      901 tagacgtcga agtaggcggc gtggtagcgc ttattgccgg gatcgttcca gaaatagagc
      961 ggcatcggcg gggcgggagc ctcacacacc agttcgccca cctggtcgcg cagcggcttg
     1021 cctgcctcgt cgtagcactc gatcttcatc cccaggcccg gagcctgcag ttggccggcg
     1081 tacaccggca gcgtgggact cccgatggca aagcacccgt tgatgtcggt gcccccagag
     1141 agtgagttga aatgcagatc ggccttgatg gcgtcgtaga cgtaggcgaa gccatccggc
     1201 gagagcgccg agccggtctg gcagatctca cgcagggccg aaagctcgta gtcctctttg
     1261 ggcaccatgc cttgagcctt caagaggctc aggtagctcg cgctggtgcc gaagactgtc
     1321 accttgagat ccgcgatcag gcgccaaagc gcgcccaggt cggggtaggc ggggttgccg
     1381 tcgaagagga ccacggtggc ccccgtcccc agggccgaca tcagccagtt ccacatcatc
     1441 cagctggccg tggtgacgta gaagatggtg tcgtcgcgcc tgagatcggc gtgcaagacc
     1501 agctccttca gctggttgat gaggatcccg ccgccactct gcacgaggca tttgggcttg
     1561 cccgtggtgc ccgaggagaa catgaccact gccgggtggt cgaagggcgc ctgctcgaag
     1621 aaaagctctt cgccggcctg atcggcgatg aactcgtccc agcggagagc gccccggatg
     1681 gaagacaggt cggcggccgg gtcgcccgcg tagtgagcca ccaccacccg ctcgatgctg
     1741 gggatgctgc gggtgaccgc ggcggcgtta gagatgcagt cgaacacccg gttcttgtag
     1801 cgatagccgt cgacggcgaa cagcaccttc ggctcgatct ggctcagccg atccgcggcc
     1861 gcctccgggc cgatgtcggt ggcgcaacta gaccacacgg cacccacggc cgtagcggcg
     1921 agcatcgcca ccgctgtctc tatgaggttc ggcaggtagc ccgccacgcg gtcgcccggc
     1981 ttgacgcccg ccgccctaag ggcgccggcc acccgagcca cggtctggta gagctcggcg
     2041 taggtcacgc gtaccggcgc agcggtctct cccacgaaga tgaaggccac ccgatcgtcg
     2101 cgatagcgca gaaggttctc ggcgaagttg agccgcgcgc cgaggaacca gttcgctccc
     2161 gggaaccggg ccaggtcgtc gatcaccgct gtgtagggcg tagaggcctg gataccgagg
     2221 aagtcccacg ccgccgccca gaaaccgggg atgtcgtcga ccgaccagcg gtggagcgcc
     2281 gcgtacgatt cgaacgtccg gccacacctc ttgccggcaa acgtggcgaa gcgcgtcata
     2341 ttcgcgtcgg cgacgcgttc cgccaacggt tcccacagca gtgtagccac agcgctctcc
     2401 tcctcgttcc cacggatgcg ccgccgggcg cggccgctcg tctatcgcac ccggactact
     2461 gtcccttgaa cgatgccttt cgcttggcca cgaacgcgcc catgccttcg ctctggtctt
     2521 cggtggcgaa gaggaccgcg aacatcgagg cctcgtagcg caggccgtcc tccttggcca
     2581 tgtcgaggcc attggcgatg gagtctttgg cgtgactgac cgcgacaagg ccgttcttgg
     2641 cgatggccgc cgccgtctcc ttggtcttcg ctagcagatc ctcgggtggg aacacttcgt
     2701 tgacgatccc ccaggcgcag gccttgtggg catcaatcat cttgccggtc aagatcaact
     2761 cgttagcctt gttgcggccg atgagccgcg gcaggttctg ggttccgcca aagccgggca
     2821 tgatgcccaa tgtgacttcc gtgagcccca gtttcgccgt ttcggaggcg tagatgaagt
     2881 cgcaggcgag cgccagttcc agcccgcccc cgagagcgta gccgttcacc gcggcgatca
     2941 ccggcttctt catgttctgg atgaagagca tgacccgctg ccctctgcgg gcgaaggcgt
     3001 gtccctggac ggagtccatc gactccacct ccttgatgtc ggccccggcc acgaaggact
     3061 tctcgccggc cccggtgatg atcaccacct tgacggccgc ctcgagctcc agctcgaaga
     3121 gggcgtactc caactccctc acgacgtcgc ggttgagcgc gttgaggctc tggggacggt
     3181 cgatggtcac ggtagcgatc ccgtctgccg tctcgacctt caggttctgg aattccactg
     3241 tgtgcctcca aggtcttgcc acagacatgc ttgtggcggg cttcaatacg tgaagaaacc
     3301 tctcccggtc ttcctcccca ggtaccccgc gttgaccgtc ttgacgagga gcggacaggg
     3361 gcggtatttc gggtccttga agccgtcata gaggacgttg gcgatggcca gcactgtgtc
     3421 gagaccgatg aagtcggcga gagccaaggg ccccatcggc tggttgaggc cgagcttcat
     3481 ccctttgtcg atgtcctctg ccgtggcgat gccctcatac agagcgaaga ccgcctcgtt
     3541 gagcatgggg atgagcaccc ggttgatgat gaagccgggg tagtcctggg agacggccat
     3601 ctccttgccc agcttgacca ccatgtcggc ggtgacctcg aaggtctcgt cgctggtagc
     3661 gtgtcccctg atcacctcga cgagcttcat gaccgggacc gggttcatga agtgcatgcc
     3721 gatcactttc tccggacgtc cggtgacggc cgcgatcttg gtgatcggaa tggaggaggt
     3781 gttggaggcg aggatcgctc cctctttgac cgtcgcgtcg agattgcgga agatgtccag
     3841 cttgaggggc tcatgctcgg tgaccgcctc aacggcgaag tccgattggg ccagatcggc
     3901 catgtcttgg gtcaccttga gccgccccat cgtctcggcg acggtagttt cggggatggc
     3961 tcccttcttc gcttgccgct ccaggttctg tcgtatggtg acgagcgcct tctcaagctg
     4021 ctccggcacg atgtcgtaca tcaccacttc gtagccttct tgggcgaaca cgtgggcgat
     4081 gccgttcccc atctgcccgg cgccgagcac gccgattctc tcgatcatcc taggttcctc
     4141 ctcgtacaca gtagccacga acggtgtctc acactcgttc gaagatggcc gcgaacgcct
     4201 cacccccgcc gatgcagagg acggccaggc cgtagcggca ctgccgtctc tgcagctccc
     4261 ggacgaccgt cgccggcagc ctcccgccgc tggcgccgat ggggtggccg agggcgcagg
     4321 cgccgccgtt gacattgacc ttgtcgatgt cgaggccgag ggacttgatc gcgatcatcg
     4381 ccaccacagc gaaggcctcg ttgatctcga agaggtcgat atcgccgatg gacaaccccg
     4441 cctgcgcgca ggccttctcg atggcgccga ccggcgcctc ggggaagtgg tcgggatgga
     4501 gactctccgt ggccgccgcg accaaccggg ccttgggggt caagccgaac ttcttgagcc
     4561 ccgcttcgtc agtgagaagc gccacggcgg ccccgtcgct gatgggggag gcgttgccgg
     4621 ccgtgatcgt gccgtctttc ttgaacgccg gacgcagctg cgtgagcttc gcgagatcga
     4681 tcttgaacgg cccctcgtcg gtgtcgaccg tcacatcgcc tttcttccct gtcttgacga
     4741 acgggacgat ctcatcccga aagacgccct ccgtcaccgc tgcctgagcc aggcggtagg
     4801 agcgaagggc gaattcgtcc tgcgcttcgc gcgagaagcc gtttcgcgtg gcgctatcgt
     4861 cggcgatgac ccccatgtgc tggccggaat agggatcctg taagccgtcg cagatcatga
     4921 ggtcgacgat ctcgccgttc cccatccgat agccggtgcg agccttcttc aggatgtagg
     4981 gggccagcga catgctctcc attcccccgg cgatgacgac ctccgaatgc cccagctgga
     5041 gagcgtcggc accgagctgg atcgccttca gaccactacc gcacaccttg ttgacggtga
     5101 gggcggcagc cttgtcgggg atacccgcag cccgcatcgc ctgccgcgcc ggcgcctgcc
     5161 cgcagccgcc cgccaacacg ttgccgacga tcacttcgtc gaccgcttct ggggcgaggt
     5221 cgaccttctc cagcagtgcc ttcatcaccg ctcccgcaag ctgcggcgcg ggaacatccg
     5281 ccagcgatcc tccgaacgaa ccgaacggag ttctcagggc gtccaccacg tacacgtctt
     5341 tcatgttctc ccctccatca gaggtgcgag ttttcccttg aatatagctt caggctgcga
     5401 gggaacacaa cctgtcgcat gtgtggacgg tggcgcatac ggaccccgac cgtatagtct
     5461 tcgtccaccg acccgcgacc gggacttggc gcgatgtgtc ggcgacgctc caagagcctc
     5521 cgggcgccgg aatccgtcgt cgacatcgtc aagcagctct ggagcagagc cgttgccccg
     5581 gaggcgagtc gcccgaaaac cgcggagtcg gcgccgacag gagacgagac gcccgcagac
     5641 ggtcgatgcc ggtcggggat cgaggaatca tgactgacgt caccaaggct ttgaagcagc
     5701 aggctcactc gctgggggcc gacctggtgg gggtggccgg gctcggttgg cagggcaaga
     5761 gcctcctcat cgtgacccgt cgcagaccac ggccggatgt ctccgtggtc gccatcaagc
     5821 gagttcacca aacgagggcg aggatgctct cgagagcaga actggccctg tcaccggagg
     5881 taaacgatgt ttctcgaact gaccgcagag cagcgactga tccaggacac agcccgcgag
     5941 ttcgccaagg cggagctgga cccgatcgcg gccacgctcg accaggaggg ggatcccccc
     6001 gccttcttgg ccaatctgcg gaagctggcc gagctcggtt tcatgggtct gaacgtcaag
     6061 gacgagtatg gcggcgctga ggcgggtgtg gtggccttca gcgtggccat caccgagatc
     6121 gctcgcgcct gcgcctcgac ggccgtgacc ctgtctgtga acaacatggt ctgcgaggtg
     6181 atccaggcgg ccggctcaga ggctcagaag aaggcgtaca tccccaagat ctgctccggt
     6241 gagttctctg cgggagcttt cgctctcacc gagaccggcg ccggctcgga tccgtccgga
     6301 gtggcgactc aggcggtcct cgacggtgat cagtgggtgc tgaacggttc caagatcttc
     6361 atcaccagcg ccccatatgc aggcgtcttc atggtctggg cagtgaccga ccggtcggtg
     6421 cccaagggga agggtatcag ctgcttcctc gtcgagggcg gcacgccggg actggtcatc
     6481 ggcaaggagg aacacaagac cgggcagcac gcgtcggcca ccaatgagct cctgttcgag
     6541 gactgccgta tcccgaaaga cgccctgatg gggaagctca acgacggctt ccgcatcgcg
     6601 gtgggggaac tggccggcgg tcgcatcggc atcggctctc tcggcctcgg cgtggggctg
     6661 gcggcgatcg actatgcgac ccagtattcg accgagcgta ctcagttcgg ccagaagatc
     6721 tccaacttcc aggcgatcca gtggatgatc gcggacacgt acaccgagct ggaggcggcg
     6781 cgtctcctgt tgatgaacgc cgccttcaag aaggagcagg gcaagccgtt cgccaaggaa
     6841 gcctccatgg cgaagatgta cgccaccgag gcggcgaatc gcgcctgcta caaggcggtg
     6901 cagatgctcg gcgggtatgg ctacacggtc gatttcccgg tcgagcgtta cgcacgcgat
     6961 gcgcggatca cagccatcta cgaagggacc aacgagatcc agcggacgat catctcccgc
     7021 gagatcttgc ggagcatcgt ctgatcaggg agcgtgccgt tcagaggcgg catcggcctc
     7081 accgatcagc aggccatcga cgcctgggtt gccgagctca acgcacggcc gcgacacgag
     7141 cgcgacgcgc tcatgggcgc atcccccttc cccgacgact ccgatcctgc aacctgagcg
     7201 gtaggtgtga ccgcatcccg cagcaggcct gcaccgctca caaggcccac ggacgagctg
     7261 gtaccatacc gccatgaagg tcgtcgtctt cggagccacc ggtcgtaccg ggagactgct
     7321 cgtggagggc gcgctggccc gcggtcacga cgtgaccgcg ttcgtgcgcg ccccaaacaa
     7381 gctcggcgcc ctgcgagacc gcgtccgggt cgtgcagggc gccgagcttg ttt
//
LOCUS       DASRMJ010000119         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_449282, whole genome shotgun sequence.
ACCESSION   DASRMJ010000119 DASRMJ010000000
VERSION     DASRMJ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449282"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..>2900
                     /locus_tag="VFD74_03430"
     CDS             336..>2900
                     /locus_tag="VFD74_03430"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK48635.1"
                     /translation="MMRHTFTELVDVTALQGLTDALHRATGIPSAVITTEGEVLTASG
                     WQRICTDFHRTHPDIEKECIQSDTAIRASLAAGDAFAVYTCPRSLTDASMPVIIEGEH
                     LANVFMGQLFTEAPDESTERFFREQSRRFGLDEGDYLEAFREIPVFSEERFRLALSFL
                     AELARSVASSGLSRLRELETTARLDAERSKYRIVADNTYDWEFWLSSEGRFSYVSPSC
                     ERVSGHTPQEFEEDPELMARIIHPDDRDLFLAHMRSALGGHTAQELTFRIIRADGGVR
                     FLEHICVPVSAEDGTFLGLRGSNRDITARKQAEEALHASEERYRTILDLSPIPWAMND
                     DDGNITFLNEAFVDTFGYTLEEIPTLADWWPRAYPDPEYRQWVSSTWKHRLDQANLEG
                     TPFDPMELRVNCKDGAVLTVLASAALFSESPGREHLVILYDITERKQAEEALRESEER
                     YRRLFELESDAIVLVDNGSGQVLEVNEAASVLYGYSREEWLSMNHTDVSAEPDSTRRA
                     AVEGERLIPIRWHRKRDGTVFPVEITAGHFEWKGRSVHVAAIRDITERTQAEEEHLRL
                     ERQLQHSQKLESLGVLAGGIAHDFNNILLSVLGNADLALDELSPSAPARENLLAITAA
                     SRRAADLCRQMLAYSGRGHFVIEPIDLDALIRDLLGLLESTISKKARLDLSLEQGLPR
                     MRGDASQLSQVVMNLVINASEAIGDNDGVITISTGVRECPREHLCAIYSDKDLPSGLY
                     LTLEVSDTGSGMDAETRDRLFEPFYTTKFTGRGLGLAAVQGIVRGHGGALELESTPGR
                     GTTFTVYLPIADTEVGTPVSASVETTADWQGRGIVLLVDDEETVRSVGRRMLERLGF"
BASE COUNT          539 a          941 c          914 g          506 t
ORIGIN      
        1 ccagcaggat ggtgcactcc cgcccgagtt ccggcatgtc ggatcgcgcg agcccaaaca
       61 ccgtttcgtc gtccacggcg gcgtagtcgt gcgcaagtac gttgcgaaag ccgacgatca
      121 cattgcgtgc gcgcacggta ctgagtgaaa ttactcaaca tgatgagtga atcgtacagc
      181 aggcgctggt tgatttcgat cgccctcgcc ctcgcgctcc actgccgagc gtacggcgca
      241 cgccacactc atcgtgctgt ccgagcggcg cagtttccac tactatggcc gcatcagggc
      301 atcttctcgc ggtcgacccg gggctggaag ggacgatgat gcgacacaca ttcacggagc
      361 tcgtcgatgt cacggcgctg caaggcctca ccgacgcact ccatagagcc acgggcattc
      421 cctccgcagt gatcaccacc gaaggcgaag tcctcaccgc gtcgggctgg cagaggatct
      481 gcaccgactt ccatcgcacg cacccggata tcgagaagga gtgcatccag agtgataccg
      541 ccatccgcgc gagtcttgcg gccggtgacg ccttcgccgt ctatacgtgc ccccgcagtc
      601 tgaccgacgc ctcgatgccc gtgatcatcg aaggcgagca cctggcgaac gtgttcatgg
      661 ggcagttgtt caccgaagcg cccgatgaga gcaccgaacg gttcttccgg gagcagtccc
      721 ggcgcttcgg cctcgacgag ggcgactacc tcgaagcctt ccgcgagata cccgtgtttt
      781 ccgaggagcg gttccgcctg gctctctcgt tcctcgccga gcttgcgcgc tcggttgctt
      841 cttcgggact cagtagactt cgagagttgg agacgaccgc cagactcgat gcggagcgga
      901 gcaagtaccg catcgtggcc gacaacacct acgactggga gttctggctt agctcggagg
      961 gtcgcttcag ctacgtgtca ccatcgtgcg aacgggtatc ggggcacacc ccgcaggagt
     1021 tcgaagagga ccccgagctc atggcgcgga tcatccaccc cgacgatcgg gacctgttcc
     1081 tcgcccacat gcgctctgcg cttggtgggc acactgcgca ggaactcact tttcgcatca
     1141 tccgagcgga cggaggggtg cgcttcttag agcacatctg cgttcctgtc tcagctgaag
     1201 acggcacctt tctcggcctc cgagggagca accgcgacat caccgcccgc aagcaggccg
     1261 aagaggcgct gcacgcgagc gaagagcggt atcggactat cctagacctg tcgccgatcc
     1321 catgggccat gaacgacgat gacggtaaca tcaccttcct gaacgaagcg ttcgtcgaca
     1381 ctttcgggta cacgctggag gagattccca ctctggcgga ctggtggccc agggcctatc
     1441 ccgacccgga gtatcgtcag tgggtgtcgt ccacgtggaa gcatcggctg gaccaggcga
     1501 acctcgaggg gactccgttc gatccgatgg agttgcgggt caactgcaag gacggcgcgg
     1561 tgctgaccgt gttggccagc gccgctctgt tctccgaatc gcccggacgg gagcacttgg
     1621 tcatcctcta cgacatcacc gagcgcaagc aggccgagga ggcgctgcgg gagagtgagg
     1681 agaggtatcg tcggctgttc gagctcgaat ccgacgccat cgtattggtg gacaacgggt
     1741 ccgggcaggt gctcgaggtc aacgaggcgg cgagcgtcct ctacggctac agtcgtgagg
     1801 agtggctctc catgaaccac accgacgtct cggcggaacc ggactcgacg aggcgggccg
     1861 ccgtcgaggg cgagagactg atcccgattc gctggcaccg gaagagggac ggcacggtct
     1921 tcccggtgga gatcacggcc ggccacttcg aatggaaggg gcggtcggtt cacgtggcgg
     1981 ccatccgcga catcaccgag cgcacgcagg ccgaggagga gcatctgcgg ctggagcgcc
     2041 aactccaaca cagccagaag ctagagagcc tgggggtgct cgcggggggt atcgcgcatg
     2101 atttcaacaa catcctgctg agcgttctcg ggaacgccga cctggctctc gatgaactct
     2161 ctccgtccgc gccggctcgg gagaacctgc tcgcgatcac agccgcctcc cgccgtgccg
     2221 ccgacctctg ccgccagatg ctcgcctact ccggtcgggg gcacttcgtc atcgaaccca
     2281 tcgacttgga cgcgctcatt cgcgacctgc tcggccttct cgagagcacc atatccaaga
     2341 aggcgcggct cgacctctcc ctggagcagg gcctgcctcg tatgcgcggg gacgcgagcc
     2401 aactctccca ggtggtcatg aatctggtga tcaacgcttc cgaagccatc ggggacaacg
     2461 acggcgtcat cacgatatcc accggggtgc gggagtgccc tcgcgagcac ctatgcgcga
     2521 tctactcgga caaggacctg ccttccgggc tgtatctcac cctcgaggta tccgacaccg
     2581 ggtcgggcat ggatgcggag accagggaca gactcttcga gcccttctac accaccaagt
     2641 tcaccggccg aggcctcggt cttgctgcgg tgcaaggcat agtgcgcggc cacgggggcg
     2701 cactcgaact cgagagcacc ccgggccgag gcaccacttt caccgtctac ctccctatcg
     2761 ccgacaccga agtgggcacg ccggtctcgg cgagcgtcga aacgacggcc gactggcagg
     2821 gccgaggcat cgtgctcttg gtcgatgacg aggagaccgt ccgctcggtg ggaagacgca
     2881 tgctcgagcg cttgggcttc
//
LOCUS       DASRMJ010000120         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_262451, whole genome shotgun sequence.
ACCESSION   DASRMJ010000120 DASRMJ010000000
VERSION     DASRMJ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262451"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..248)
                     /locus_tag="VFD74_03435"
     CDS             complement(102..248)
                     /locus_tag="VFD74_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48636.1"
                     /translation="MGLVVVLIVLALVLGGVGLFVEALRWVLIIALILLVVSIVTGFM
                     RRSR"
     gene            complement(341..1572)
                     /locus_tag="VFD74_03440"
                     /pseudo
     CDS             complement(341..1572)
                     /locus_tag="VFD74_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1945..>2287
                     /locus_tag="VFD74_03445"
     CDS             1945..>2287
                     /locus_tag="VFD74_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HZK48637.1"
                     /translation="MIGNYVPRRCGIATFTTDLTESLGAEAPESEVWAAAMNDTPDGY
                     SYPSRVHFEMSEPSRGDYLKAAEFLNISGVEVASLQHEYGIFGGPAGEFILDLIGQLR
                     MPVVTTLHTVLR"
BASE COUNT          439 a          733 c          731 g          384 t
ORIGIN      
        1 aatgggaccg gtcgcacggt cctcgttcac ccgactcggc gagagcgggg gacgaccgtc
       61 aacggtactc gatcatcggc gccccatgaa acggtactcg atcatcggct gcgccgcatg
      121 aaacccgtca cgatgctcac aacgagcaga atgagggcga tgatgagcac ccaccgcagt
      181 gcttccacga acagaccgac tccacccaag actaacgcca aaacaatcaa cacgaccacg
      241 agtcccatag cgatctcctt ttttctctag tatctccttg atggcgtccc aggagatggt
      301 gtaagaacca tcgtggttct cgaaggtctc cagcataggg tcagtggacc ggaatggcaa
      361 caggatgaag gagtgggcga gcgcttgcct gatcgtcgcc cgcgtagatt tgggcgaggg
      421 acgtctggct catgtaccgg cggctgacgg cccactcgtc gttttgttcc aggaggaggg
      481 caccgacgag gcgcagcaga ctgcgttagg gaagatcccc accaccttgg tgcgccgggc
      541 gatctcctcg ttcaggcgct cgagcgggtt ggtcgagccc agcttgcgcc agcgctcccg
      601 ggggaagtcc atgtgggcga ggatctcgtc ttcagccgca agcaggcacg cggcggcctt
      661 gccgaactgg ggctcgagcc gttccgtgag tgccctgagt tcggcccggg cggcctctcc
      721 gtccggttgc tcgaagatga cccgggtggc cgcgagcacc atgccctgag cctccttgcg
      781 cacgtgaccg agcaggttgc gctggaagtg cacccggcag cgttgccaag acgcacccac
      841 gaagacttcc gcggtcgccc gtttcaggcc ctcgtgggca tcggagatga caagcttcac
      901 cccagtgaga ccacgggcga cgagaccgcg caggaacccc ttccagcagg cgtagtcctc
      961 ggagaggaag acgtccagac cgaggacctc ccgctcgccc gtctcggcca ccccgtaggc
     1021 gacgacgagg gccatgctga cgacgcgact cttctcgtca cgcaccttct cgtagcgagc
     1081 gtccagaaag agataggggt agcgaccgac caaaggcctg cttcggaacg cctccacctg
     1141 ctcgtcgagc cgggcggcga tgcgggagac ctcgctcttc gagaccccgt ccaccccgag
     1201 ggcacgcacg agctcgtcca ccttgcgggt ggagacgccc tggacgtagg cttcgcaggt
     1261 cgtgttcagg agcgcctcct ccgagcgccg gcgcggttcg aggaaggcgg ggaagtacgt
     1321 gccctcgcgc aacttgggga tgggcaaagc gagactgccg gcgcgggtgt cgaagcggcg
     1381 ctcgcggtag ccgttccggt gtgtaacccg ctcggcggtg cgctcgccca gggaggcgcc
     1441 ggtgatccgg cccacctcga cctccatcac ctcccggagc acggtgagca ccgcgtcgcg
     1501 caccaggtcg acggttctcc cttccaggag cttgtcgagc agctgttcgg gtgccaggat
     1561 ggaatcggcc atcgtgtctc gttctcgtcg agcttcagca acccaacgag aacacacgat
     1621 ggcttcttct tgcaacgggg ccgacagagg cggctaccgg cttgcgctct tacaccacgt
     1681 cctgggacgc gatcggggaa tcgctcttca agagggggcg gcagcgtatg cgtcgtgggt
     1741 gtttgagaaa cagactggct gggaaagaga gtcagcactt actcgtcagt catgcccttg
     1801 tattcggaag ccactccaga atgaccggcg ttcgaacgac gcggtcgaga gccagcaaac
     1861 tttgcgcgcc aagcgctcgt gggtaggaac aagagggcaa ctgtgccgga ccagcaaccc
     1921 cattcacgcc ccactcggat tgcagtgatc ggcaactacg tcccgcggcg ctgtggaatc
     1981 gccacgttca caacggacct gacagaatcc ctgggagcgg aggcacccga gagcgaagtc
     2041 tgggctgccg ctatgaacga cacgcccgac ggctattcct accccagccg ggtgcacttc
     2101 gaaatgagcg agcccagccg tggggactac ctgaaggcgg cggagttcct caacattagc
     2161 ggagtagagg tcgccagtct gcagcacgaa tacggcatct tcggcgggcc ggcaggcgag
     2221 ttcatcctcg atctgatcgg ccagttgcgt atgccggtcg tgaccacgct ccacaccgta
     2281 ctcaggg
//
LOCUS       DASRMJ010000121         7793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_87864, whole genome shotgun sequence.
ACCESSION   DASRMJ010000121 DASRMJ010000000
VERSION     DASRMJ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87864"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFD74_03450"
     CDS             complement(<1..722)
                     /locus_tag="VFD74_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZK48638.1"
                     /translation="MNPSAKPSGNPSRTPSRRPVAAPAGNVVANAAAGLGEIIYGRNP
                     VREALRGRRRVRRAWVLPGRGGDDLAAEVASWAAAAGVASPPISPLPAQELVAAAGGP
                     DHQGLVAEVDPYAYASLGTVLDGFDLVVALDRIQDPHNLGAIIRTAEVAGAAVIIPRH
                     RTAAVTGAVVKASAGATEHAAVAQVRNLTDFLDQAKQAGFWTYGADAAAASAHSAQDY
                     RGKTVFVVGSEGEGLGRRVAQA"
     gene            complement(753..2216)
                     /gene="cysS"
                     /locus_tag="VFD74_03455"
     CDS             complement(753..2216)
                     /gene="cysS"
                     /locus_tag="VFD74_03455"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZK48639.1"
                     /translation="MPQLYDSLSQQKSDFVPRDADHVTMYFCGPTVYNYIHLGNARPY
                     VISMVAKRYFESLGYRVTLVENITDIDDRIIQKAAAEGRSATEVASEFAKAYIEDTNG
                     LGLGRPDIEPYATEHIPEIIDLVQKLVEAGHAYQAGTDVYYDVDSFAGYGRLSKQQIA
                     EMRHGARITPGEDKADPLDFALWKGAKPGEPAWESPWGPGRPGWHIECSAMSLKYLGS
                     GFDIHGGGRDLIFPHHENEIAQAEGALGGTFVRFWVHNGMLNTRDEKMSKSLGNIFQL
                     RQALAEYRPEVLITFFVGSHYRSPMDFSRELLEEAGHQAWRLRNLFRALDDFIRGEPA
                     DPSPAGADGAPGASFEPSSPASKKDAELVAQADSRRAAFDAAMADDLNTAGALGEVFA
                     LAREVNSALASRSVSAIAASALRKRIGAMVYILGLEAATRVDDAVPADVLEMAAQRLA
                     SRAARDFAEADRLRDAILARGFEVRDGADGYKVVPAE"
     gene            complement(2216..3682)
                     /gene="gltX"
                     /locus_tag="VFD74_03460"
     CDS             complement(2216..3682)
                     /gene="gltX"
                     /locus_tag="VFD74_03460"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZK48640.1"
                     /translation="MSDARVRFAPSPTGTLHIGSARTALYNYLFARHLGGTMVLRVED
                     TDLKRSRHEHELSIVRDLHWLGLQADEGPDVGGPYGPYRQSERGELYEAGIQKLLASG
                     ATYRCFCSQERLDELKAAQLAAGKMPKYDRSCLTLSADEVETRLDAGEEATVRFRVPK
                     GDITFDDLIHGPITFSSDVIGDFIVRRTDGGFAYNYAVVIDDAGMRITHVVRGEDHIT
                     NTARQLMLYQALGEQAPVYAHHSLIHGADGGKLSKRHGATSIGEFRDMGYLSASVVNY
                     LALLSWSSGDEREKFTLDELVAEFDMKRVSKSPAIFDLDKLNWLNGLYIRELSPEQLA
                     EAVRPYLREAELEFEEGQRDVVAGAIQSNLVVLGDAPTYAEIFADEPILTASSFLEAL
                     HEPGADAVLAMLAHELESRDREYFSPDQARTILKVVADECKERGIKGKSIYKPLRVAL
                     TARDQGPELFYLVAGLGRSRIMARLRTAMSYVAQRGSE"
     gene            complement(3754..4266)
                     /gene="ispF"
                     /locus_tag="VFD74_03465"
     CDS             complement(3754..4266)
                     /gene="ispF"
                     /locus_tag="VFD74_03465"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZK48641.1"
                     /translation="MSGAPRVRVGLGVDAHAFAPERPLVLGGVRLREHDGLAGHSDAD
                     VLVHALMDGLLGAAGLEDIGHYFPDSDPAFAGADSVELLRRVVTILGGGGWRVVNVDV
                     VVICEQPRIAPHRARMKAALAPVLGVDEGCVGIRGTTTEGMGFTGRGEGIAAQAVVLL
                     EGTALELGVA"
     gene            complement(4263..5051)
                     /gene="ispD"
                     /locus_tag="VFD74_03470"
     CDS             complement(4263..5051)
                     /gene="ispD"
                     /locus_tag="VFD74_03470"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZK48642.1"
                     /translation="MGREHASTSPAGGVAPAPAGETAPGGRHHRLGLIVAAGGYGHRL
                     AAGGPKQYVPLLGVPMVRRTIAALDACSAVHSLVVVVNAEDVDYCAEEIVGDRFAKVV
                     AVVGGGAERALSVRNGLAALARAGPFDLVGVHDGARPLITCDEVARVCARLVADPGLA
                     GAILAAPSVDTLKRVDGQGIVVETPERGRLWRALTPQVFRWAAFAAAYDQADETLVAA
                     TDDSSLVEAAGGRVAVVEGSPENLKVTNSADVWVAERLLSERVR"
     gene            complement(5053..6105)
                     /locus_tag="VFD74_03475"
     CDS             complement(5053..6105)
                     /locus_tag="VFD74_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZK48643.1"
                     /translation="MRGVVALVRLLLTIVGGVAGYEASNLGLSYASLSGTYSLLLLAL
                     GVVVGVGIGFILGGVLGRQVLRLGSWVDRVTVRVSAAALIVTSIGLVLGLGIAALASL
                     AVKDLPVVGVFLLPVIYAVCGYVFASLAYSRHSDIGRVVGFKNLGSPDSAGGHMPKIL
                     DTSVIIDARIGDIVPTYFLEGDLIIPGFVLEELQSIADSSDPLRRARGRRGLECVSYL
                     QTQHERVVIADQDFPGLAEVDMKLIKMAKEMGAAILTTDYNLNKIAQIQGVVVLNVNE
                     LANALKPVVLPGESLAVRVIREGKEEDQGVAYLDDGTMVVVEGGRKKVGQTIEVEVTS
                     VLQNPSGKMVFTKLAS"
     gene            complement(6142..6645)
                     /locus_tag="VFD74_03480"
     CDS             complement(6142..6645)
                     /locus_tag="VFD74_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HZK48644.1"
                     /translation="MFKVGDKVVYPHHGAGRILEVAERTVCGEPKEYMTIRILHNDMT
                     VMVPVENAERAGIRSVIDEILVHEVIGVLHDDPTKMPMNWNRRLKHNRDKIKSGDIFE
                     VADVVRNLAIRDAQKGLSTGEKQMFGRVKKILASELMYAMRVSEDEALTNLEGVLEEV
                     CVRATCE"
     gene            complement(6666..7748)
                     /gene="disA"
                     /locus_tag="VFD74_03485"
     CDS             complement(6666..7748)
                     /gene="disA"
                     /locus_tag="VFD74_03485"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HZK48645.1"
                     /translation="MGKKHVFTSERLVSALQLVSPGTVIREALDNIMRARTGALVVFA
                     DEEQVAPLVTGGIPIGVEVTPTVVYELAKMDGAILLDRHGLRITTANVHLMPDPSISS
                     VETGTRHRTAERVAKQIDALVVSISAARDVISLYIDGTRYVLQEIRVILDKANQGLQA
                     LEKYKSRLTQVSTSLSALEFEDAVTLYDVLSVLQRSEMVLRISREIERYIIELGAEGR
                     LIRMQLEELMVDVREDQRSVLLDYLPDFADHSPEEVTRRIGLLHSEELLLLGRVGELL
                     GYGPDVDALELHVSARGYRMLRKIPRLPKGVITNLVARFSSLEEIINASTEELAQVEG
                     VSQARAHDIKEGFLRLRELNLLERYG"
BASE COUNT         1328 a         2651 c         2485 g         1329 t
ORIGIN      
        1 caggcctgcg cgacacggcg acccaggccc tcaccctctg agcccacgac gaagaccgtc
       61 ttgccgcgat agtcctgggc gctgtgcgcg gaggcggcgg ccgcgtctgc tccataggtc
      121 cagaagccgg cctgtttggc ctggtcgagg aagtccgtga ggttgcgcac ctgggccacg
      181 gccgcgtgtt ccgtggcccc cgcggacgcc ttgaccacag cgcccgtgac cgcggccgta
      241 cggtgccgag ggatgatgac ggcagcgccg gcgacttccg cggtgcggat gatggcgccc
      301 agattgtgcg ggtcctggat acggtccagc gccaccacca gatcgaaccc gtcgagcacc
      361 gtccccagag atgcgtatgc ataggggtcg acctccgcga ccaggccctg atggtccgga
      421 ccacccgcgg ccgccaccaa ctcctgtgcc ggaagcggcg atatgggcgg gctcgcgaca
      481 ccggccgccg ccgcccacga cgccacctcg gcggccaggt catcgccgcc gcggccgggc
      541 agcacccacg cacggcgcac acgcctgcgg ccgcgcagcg cctcgcgcac ggggttgcga
      601 ccgtagatga tctcgccaag gccggcggcg gcattcgcaa cgacgttccc ggcgggtgcg
      661 gcgacgggcc tacgagaggg ggtccgagac gggttccccg agggtttcgc ggaagggttc
      721 atccgaatgt cctcgacatc agtcagccga cgctactcgg ccggcacgac cttgtacccg
      781 tcagcgccgt cgcgcacctc gaagccgcgg gccaggatgg catcacgcaa ccggtcggcc
      841 tcggcgaagt cacgagccgc gcgactggcc aggcgttgcg cagccatctc cagcacgtcg
      901 gcgggcacgg cgtcatcgac ccgcgtggcg gcttcgagcc ccaggatgta gaccatggcg
      961 ccgatccgct tgcgcagtgc tgaagccgcg atggcggata ccgatcgcga ggcgagagcg
     1021 gagttcacct cacgggccaa ggcgaacacc tcgccgagcg ctccggccgt gttcagatca
     1081 tcggccatgg ctgcatcgaa ggcggcacgg cggctgtcgg cctgggccac cagttcggcg
     1141 tccttcttgg aggccgggga cgaaggctcg aacgatgcgc ccggcgcacc gtcggcgcct
     1201 gcggggctgg ggtccgcagg ttcaccgcgg atgaagtcgt ccaaggcccg aaagaggttg
     1261 cgcagccgcc acgcctgatg gcccgcttcc tccaggagtt cccgactgaa atccatgggg
     1321 ctgcggtagt ggctccccac gaagaaggtg atgaggactt cgggacgata ctccgcgagc
     1381 gcctgccgaa gctggaagat gttgcccagg ctcttcgaca tcttctcgtc gcgcgtgttc
     1441 agcatgccgt tgtgcaccca gaaacgcacg aacgtgccgc cgagggcgcc ctccgcctgg
     1501 gcgatctcgt tctcgtggtg cggaaagatg agatcgcgtc caccgccgtg gatgtcgaaa
     1561 cccgaaccca gatacttgag gctcatggct gagcactcga tgtgccatcc gggacggccc
     1621 ggaccccaag gactctccca ggcgggctcg cccggtttgg cgcccttcca gagcgcgaag
     1681 tcgagcggat cggccttgtc ctcgccgggt gtgatgcggg ctccgtgtcg catctcggct
     1741 atctgctgct tcgagaggcg accgtatccg gcgaagctgt cgacatcgta gtagacatcg
     1801 gtgccggctt ggtaggcgtg cccggcctcc accagcttct gcaccaagtc gatgatctcg
     1861 gggatgtgct ccgtggcgta gggctcgatg tcgggccgac ccaggcccag tccattggta
     1921 tcctcgatgt aggccttggc gaactccgag gccacctcgg tggccgagcg cccctccgcg
     1981 gcggccttct ggatgatgcg gtcgtcgatg tcggtgatgt tctcaacgag cgtgacccga
     2041 tatcccagtg actcgaagta gcgcttcgcc accatcgaga tcacgtaggg ccgagcattc
     2101 cccaggtgga tgtagttgta gaccgtcggc ccgcagaagt acatggtcac gtggtccgcg
     2161 tcgcggggca cgaaatcact cttctgctga ctcagtgaat catagagttg cggcatcact
     2221 cactcccgcg ctgtgccacg tagctcatgg cagtccggag acgcgccatg atcctgctcc
     2281 gaccgagacc ggcgaccagg tagaagagtt ccggcccctg gtcacgggcc gtgagggcca
     2341 cccgcagcgg cttgtagatc gacttgccct tgatgccccg ctccttgcat tcgtcggcga
     2401 cgaccttcag aatcgtgcgc gcttgatcgg gcgagaagta ctcccgatca cggctctcga
     2461 gttcgtgcgc cagcatcgcc aacaccgcgt ccgcgccggg ctcgtgcagc gcctccagga
     2521 agctggaggc cgtcaggatg ggctcatccg cgaagatctc ggcgtaggtg ggagcgtccc
     2581 caagaaccac gaggttcgat tggatggcgc ccgcgacgac gtccctctgc ccctcctcga
     2641 actccagctc agcctcgcgg agatacggcc gcaccgcctc ggccagttgc tccggcgaca
     2701 gttcgcggat gtagagaccg ttgagccaat tgagcttgtc gaggtcgaag atcgcagggc
     2761 tcttggagac acgcttcatg tcgaactcgg ccacgagttc gtcgagggtg aacttctcgc
     2821 gctcgtcgcc cgaactccag gacagaaggg ccaggtagtt gaccaccgag gcggagaggt
     2881 agcccatgtc acggaactcg ccgatggagg tggcgccatg acgcttcgag agcttgccgc
     2941 cgtcggcgcc gtggatgagg gagtgatgcg cgtagaccgg cgcctgttcg ccgagagcct
     3001 ggtagagcat gagctggcgg gccgtgttgg tgatgtggtc ttcgccccgc accacgtgcg
     3061 tgatccgcat gcctgcgtcg tctatgacga ccgcgtagtt gtaggcgaag cccccgtcgg
     3121 tgcggcgcac gatgaagtcg ccgatcacgt ccgacgaaaa ggtgatcggc ccgtggatga
     3181 ggtcatcgaa ggtgatgtcc cctttgggca cacggaagcg cacggtggcc tcttcccccg
     3241 cgtcgagccg agtctcaacc tcgtcggcgc tcagtgtgag gcacgaccga tcgtacttcg
     3301 gcatcttccc cgccgccagc tgtgcggcct tgagctcgtc gagccgctcc tgcgaacaga
     3361 agcagcggta ggtcgccccc gaagccagca gcttctggat ccccgcttcg tacaattcgc
     3421 cgcgttcgct ctgacggtag gggccgtagg ggccgccgac atcggggcct tcatcggcct
     3481 gcaggcccaa ccagtgcagg tcgcgaacga tgctgagttc gtgctcgtgc ctcgagcgct
     3541 tgaggtcggt gtcctcgaca cgcagcacca tggtgccacc caggtgacgg gcgaacaggt
     3601 agttgtatag agccgtgcgg gcactcccga tgtggagggt gccggtgggg ctcggggcga
     3661 atctcactcg tgcgtcactc atggggctcc ctgcgatgtg tgcaagccgg ccgctgaccg
     3721 cgccggacac tcgttcaaga tggattcagg tccctatgct acaccaagtt caagcgcggt
     3781 cccttcgagc aacacgaccg cctgcgccgc gatgccctcg cctcgccccg tgaaccccat
     3841 cccctcggtc gtggtgcctc tgatgcccac gcacccttca tccacaccca gcacgggggc
     3901 cagcgcggcc ttcatacgcg cgcggtgcgg agcgatgcgc ggttgctcgc agatcacgac
     3961 gacatcgacg ttgaccaccc tccagccacc cccgcccagg atcgtcacca cccggcgcag
     4021 tagctcgacg ctgtcagcgc cggcgaaagc ggggtcggag tcggggaagt agtgcccgat
     4081 gtcttcgagc cccgcggctc caagtagacc gtccatgagg gcatgcacga gaacgtctgc
     4141 gtcgctgtgt ccagcgagac catcgtgttc gcgcaggcgg acccctccca gaacgagcgg
     4201 tctctcgggc gcgaacgcgt gcgcgtcgac tccgagcccc acccggacgc gtggggcgcc
     4261 gctcaccgga cacgctccga aagcaaccgc tcggccaccc atacgtccgc ggagttggtc
     4321 accttgaggt tctcgggcga cccttcgacc actgccactc gaccgcccgc cgcttccacc
     4381 agcgaagagt cgtcggtggc ggcgacgagc gtctcgtcgg cctggtcgta tgccgcggcg
     4441 aacgcggccc agcggaacac ctggggggtc agcgcccgcc agaggcggcc gcgctcgggc
     4501 gtctccacca cgatcccctg gccgtccaca cgcttgaggg tgtccacgct gggcgcggcc
     4561 aggatcgcac cggccaggcc cggatcggcg accagccgag cgcacacccg cgctacttca
     4621 tcgcaggtga tcagtggtcg ggctccgtca tgcacgccta ccagatcgaa ggggcccgca
     4681 cgggctaggg cggcgagacc gttgcgcacg gagagggcgc gctcggctcc accgcccacc
     4741 actgccacga ccttcgcgaa gcgatcgccc acgatctcct cggcgcaata gtccacgtcc
     4801 tcggcgttga ccacgacgac caggctgtgg accgccgagc aggcgtcaag ggccgcgatg
     4861 gtgcgacgga ccatgggcac gcccagaaga gggacgtact gcttgggtcc gccggccgcc
     4921 agccggtggc cgtaaccgcc cgcggcgacg atgagaccca aacggtggtg gcggccgccg
     4981 ggcgcggttt cccccgccgg cgcgggtgcg acgccgcccg ccggcgaggt ggatgcgtgc
     5041 tctcggccca cgtcaggacg ccagcttggt gaacaccatc ttccccgacg ggttctggag
     5101 caccgacgtg acctccacct cgatggtctg gcctaccttc ttccggccgc cctccacgac
     5161 caccatggtg ccgtcgtcga ggtaggcgac tccctggtct tcttccttgc cttcgcggat
     5221 cacgcgcacc gccaggctct ccccgggcag cacgactggc ttcagtgcat tggcgagttc
     5281 gttcacgttc agcacgacca ctccctggat ctgggcgatc ttgttcaggt tgtagtcggt
     5341 ggtgagtatc gcggcgccca tctccttggc catcttgatg agcttcatgt ccacctcggc
     5401 caaaccgggg aagtcctggt cggcgatgac cacgcgctcg tgctgcgtct gcaggtagct
     5461 gacgcactcg aggccgcggc ggccccgggc ccgcctgagt gggtcggagg agtcggcgat
     5521 cgactgcaac tcctcaagca cgaagccggg gatgatcaag tcgccctcga ggaagtaggt
     5581 gggcacgata tcgccgatgc gtgcgtcgat gatcaccgac gtgtctagga tcttgggcat
     5641 gtgaccgccg gcggagtccg ggctcccgag gttcttgaag cccacgactc gtccgatgtc
     5701 cgagtggcgc gagtaggcca aggaggcgaa gacgtacccg cagacggcgt atatgacggg
     5761 cagcaggaag actccgacca ccggcagatc cttgacggcc aggctcgcaa gtgccgcgat
     5821 cccaagaccg agcacgagcc cgatactcgt gacgatcagc gccgccgcgc tgactcgcac
     5881 cgtgactcta tcgacccaag atccgaggcg tagcacttga cgccccagca caccacccag
     5941 aatgaaacct atgcctacac cgacgacaac gcccagggcc aagagcaaca gcgaataggt
     6001 acctgataag gacgcatatg aaagccccag gttcgatgcc tcgtagcccg cgaccccccc
     6061 gacgatcgtc aggagcaggc ggaccaaagc cacgacacct cgcataccct ctcctctcct
     6121 cttgctgccc agcacctccg cctattcgca ggtggcccgc acgcagacct cttccagtac
     6181 tccttcaaga ttggtgagag cctcatcctc ggacaccctc atggcataca tcagctcgga
     6241 ggctaggatc ttcttgacgc gaccgaacat ctgcttctcg cccgtcgaca gccccttctg
     6301 agcgtccctg atggctagat tgcgcacgac atcggccacc tcgaagatgt cgcccgactt
     6361 gatcttgtcg cggttgtgct tgagccggcg gttccagttc ataggcatct tggtggggtc
     6421 gtcgtgcagc acgccgatca cctcgtgcac caggatctcg tcgatgaccg aacgaatacc
     6481 cgcccgttcg gcgttctcca ccgggaccat caccgtcatg tcgttatgga ggatgcggat
     6541 ggtcatgtat tccttaggct caccgcagac cgtcctctcc gcgacttcaa ggatccgacc
     6601 cgcaccatgg tgagggtaga ccaccttgtc ccccaccttg aacacacagc actcctccgt
     6661 acccatcatc cgtacctctc tagcagattg agctcccgta gcctcaagaa cccctctttt
     6721 atgtcgtggg cgcgagcttg gctcaccccc tccacctggg cgagttcctc cgtcgacgcg
     6781 ttgatgatct cttcgaggct ggagaacctg gccaccaagt tggtgatgac acccttgggc
     6841 aaccgcggga tcttgcgcaa catgcgatag ccccgggcag acacgtgcag ctcgagcgca
     6901 tcgacatccg gcccgtagcc gagcagttcg cccacgcgcc ccagcagaag cagctcctcg
     6961 gagtggagca gaccgatgcg cctcgtcact tcctcggggg agtggtcggc gaaatcgggc
     7021 agatagtcga gaaggacgga gcgttggtct tcgcggacgt cgaccatgag ctcttcgagc
     7081 tgcatacgga tcaagcgccc ctcggccccg agctcgatga tgtagcgctc tatctcccgc
     7141 gagatgcgca ggaccatctc ggagcgctgt agcacgctga ggacgtcgta cagggtcacc
     7201 gcgtcctcga actccagcgc gctcaacgaa gtggaaacct gggtgagccg cgacttgtac
     7261 ttctcgagag cctggagccc ctggttcgcc ttgtccagga tcacgcggat ctcttggaga
     7321 acgtagcgcg tgccgtcgat atagagcgag atgacgtcgc gcgccgccga gatcgagacc
     7381 acgagggcgt cgatctgctt ggcgacccgc tccgcggtgc gatggcgcgt gcccgtctcg
     7441 acggacgaga tgctgggatc gggcatcaag tggacgttgg cggtggtgat gcgcagcccg
     7501 tggcggtcga gcaggatggc gccatccatc ttggccagtt cgtacacgac cgtcggggtg
     7561 acctcgaccc cgatcgggat gcctcccgtg accagcggcg ctacctgctc ctcgtcggcg
     7621 aagacgacca gcgctccggt tctggcgcgc atgatgttgt cgagcgcctc gcggatcacg
     7681 gtgcccgggg acaccaactg cagcgcagag accaaccgtt cgctcgtgaa aacatgcttc
     7741 ttacccaatg agacccctca cgacgtcctg tatgttcgtg accggttcca gcg
//
LOCUS       DASRMJ010000122         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_9455, whole genome shotgun sequence.
ACCESSION   DASRMJ010000122 DASRMJ010000000
VERSION     DASRMJ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9455"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFD74_03490"
     CDS             complement(<1..986)
                     /locus_tag="VFD74_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZK48646.1"
                     /translation="MATAARPTARRALFVGALLVGVPALVVAALALVRYALLMRAPRA
                     EDVAPPDNLRPPALHPVLIINPKSGGGKVEELHLVDEAQKRGIEPVVLHPGDDLLQLA
                     RDAVTRGADVLGMAGGDGSQAAVAAIAAESDVAFVCVPVGTRMNFARDLGLDIEDPAG
                     SLDAFGASVEKRIDLGRVNDRVFVNNVSLGIYGTIVQSPDYRDAKLKTAAEMLPDLLR
                     EGALSKLTFKGPDGTEHSGAQLIQVSNNPYELESLAALAGRPRLDTGTLGLVAVTVNG
                     PQEALEFAALEMASRVCSFPGLLQWHDRSFEVRSDEAVDIAIDGEAATVPSP"
     gene            complement(992..1591)
                     /locus_tag="VFD74_03495"
     CDS             complement(992..1591)
                     /locus_tag="VFD74_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZK48647.1"
                     /translation="MSARDHTCDPAVVFRSLGGLLSELRAVDHAVYAAVAVTRTPALD
                     VPIRRLSTAADYSRLWFAIAGLLALVGGRTGRRAALSGAASIGVTSAAVNLGLKAVGR
                     RRPDREQHGVPTGRHVRMPTSTSFPSGHSAAGFAFANAVSAEVPSLALPLHLLAFFVA
                     YSRVHTGVHYPSDTIAGGIVGGAVGQTVASLTRHRQRRR"
     gene            complement(1627..>2214)
                     /locus_tag="VFD74_03500"
     CDS             complement(1627..>2214)
                     /locus_tag="VFD74_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HZK48648.1"
                     /translation="LAKTADFRSVERVALITPADSRNVVIFPEKIGGRYVRLDRPHSE
                     ISPWGIWITYSPDLIHWGDSRVVMQPVPYHWDEQKIGPGATPIKTPRGWLHIYHGVYP
                     TMAGAVYRLGVALHDLADPAQLLGVADEWILEPEDPWERVGYVPNVVFTCGAIPEDDG
                     TVKIYWGGADTVMCTGTALIAELVDLCLERPRSVL"
BASE COUNT          368 a          744 c          743 g          359 t
ORIGIN      
        1 atgggcgacg ggaccgtagc ggcttccccg tcgatcgcga tgtcgacggc ctcgtctgat
       61 cggacctcga agctccgatc gtgccactgc agcaggcctg ggaagctgca gaccctgctt
      121 gccatctcga gtgccgcaaa ctccagcgcc tcctgggggc cgttgaccgt gacggcgacc
      181 agacccaggg tgccggtgtc gagccgaggg cggccggcga gcgcggcgag actctcgagc
      241 tcgtacggat tgttggagac ctgaatgagc tgtgccccgc tgtgctctgt gccgtccggt
      301 cctttgaacg tgagcttgga gagcgccccc tcgcgcagca ggtccggtaa catctcggcg
      361 gccgtcttca gcttggcgtc cctgtagtcg ggcgactgga cgatggtgcc gtagattccc
      421 agggacacat tgttcacgaa gacccgatcg ttcacgcgtc ccaggtcgat acgcttctcc
      481 acgctcgccc cgaaggcgtc gagcgacccg gccgggtcct cgatatccaa gccaagatcg
      541 cgcgcgaagt tcatcctggt gccgaccgga acgcagacga aggccacgtc gctctcggcg
      601 gctatggcgg ccacagctgc ctgtgacccg tcccctccgg ccatccccag gacatcggct
      661 ccccgtgtga ccgcatcccg ggccagctgc agcaggtcgt ctcccggatg cagcacgacc
      721 ggctcgatgc ctcgtttctg agcttcgtcg accaggtgca gctcttctac cttcccgccc
      781 ccggacttcg gatttatgat cagaaccggg tgcagggccg gcggccgtag gttgtcgggt
      841 ggggccacgt cctccgcgcg cggtgcacgc atgagcaggg catagcgcac gagggccaga
      901 gcggcgacaa ccagagcggg caccccaacg agcaaggctc cgacgaaaag ggcccgacgg
      961 gcggtgggtc ttgcggcggt ggccatgtct gtcaccttcg gcgttgtctg tggcgggtga
     1021 gcgaggccac ggtctgtcct accgctcccc ccacgatccc gccggcgatg gtgtcacttg
     1081 ggtagtgcac tccggtgtgc acccgagagt aggccacgaa gaaggccaga agatgcagcg
     1141 ggagtgcgag actagggact tcggcgctca ccgcgttcgc gaaggcgaag cccgcggccg
     1201 aatggcccga ggggaacgag gtcgaggtcg gcatgcgcac atgtcggccc gtgggaacgc
     1261 cgtgctgctc gcggtcgggc cggcgtcgcc ccactgcctt gaggcccaga ttgactgcgg
     1321 ccgacgtcac gcctatggag gctgctcccg ataacgccgc ccgccgtccg gtccgccccc
     1381 cgacgagcgc gagcaggccg gcgatggcga accagagccg agaatagtcc gcagccgtgg
     1441 agaggcggcg aatggggacg tcgagggcgg gcgtgcgcgt gacggcgacg gctgcgtaga
     1501 ccgcgtgatc taccgcgcgc agttcgctaa gcaatccccc cagagagcgg aacaccaccg
     1561 cagggtcgca cgtgtgatcc cgggcagaca tggcacgcag tctaccatcg gccgtcgcgt
     1621 cacgctctag agaaccgatc gaggcctctc caaacacagg tccaccaact cggcgatgag
     1681 cgccgtgccg gtacacatca ccgtgtcggc tcccccccag tagatcttca cggtgccgtc
     1741 gtcctcgggt atggcaccgc aggtgaacac cacgttcggc acgtagccca cccgctccca
     1801 gggatcctcg ggttcgagga tccactcgtc ggccactcct agcaactgcg ccgggtcggc
     1861 gaggtcgtgc agagccacac ccagtcggta gacggcacct gccatggtcg ggtagacgcc
     1921 gtgatagatg tgcaaccagc cccgcggcgt cttgatgggt gtggcgcctg ggccgatctt
     1981 ttgttcgtcc cagtgatacg gaaccggctg catcaccacc ctcgagtcgc cccagtggat
     2041 caggtcggga gagtaggtga tccagatgcc ccagggcgag atctccgagt ggggccggtc
     2101 caggcggacg tagcggccgc cgatcttctc cgggaagatc accacgttgc ggctgtcggc
     2161 cggggttatg agcgccaccc gttccaccga gcggaagtcg gccgtcttgg ccag
//
LOCUS       DASRMJ010000123        12507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_371291, whole genome shotgun sequence.
ACCESSION   DASRMJ010000123 DASRMJ010000000
VERSION     DASRMJ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371291"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..510)
                     /locus_tag="VFD74_03505"
     CDS             complement(16..510)
                     /locus_tag="VFD74_03505"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HZK48649.1"
                     /translation="MRRPRVPTERGGMWKVIEALALTGTIATVVIAGAAWTSLPARIP
                     THFGGDGTPDGWGGRWALLVMPVVGVVMYAGLTILERYPHIYNYPVRITEENAGRQYR
                     LAVDLITLLKLEMVWMFATLTWGTVRVARGDADGLGTFSVLFYLAALFGTITVYMVRA
                     VRGR"
     gene            624..2090
                     /gene="guaB"
                     /locus_tag="VFD74_03510"
     CDS             624..2090
                     /gene="guaB"
                     /locus_tag="VFD74_03510"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZK48650.1"
                     /translation="MIDWVRKFEAEGLSFDDVLLIPAESRILPKGTDVRTRLTKTIEL
                     NIPLLSAAMDTVSETALAIALAREGGLTIIHKNQSIERQADMIRKVKRSEAGMIVDPI
                     TLPPTATYGDAEALMAEYKISGVPIVHPDGTLAGIVTNRDTRFEHDLSRPIRELMTSE
                     NLVTVPVGTTLDQALHQFKVHKIEKLLVVDDQFKLRGLITIKDIMKKIEFPSACKDEL
                     GRLRVGAAVGVAKDTDARMEALVEAQVDVACIDTAHGHSRGVLDTILRVRENYPDVQL
                     IAGNVGTAAGAQALIDRGIDGIKVGIGPGSICTTRVVTGCGVPQITAVSECARVADEA
                     GIPIIADGGVRYSGDLVKALAAGANSVMIGSLFAGTTESPGERILWEGRSYKVYRAMG
                     SVSAMSEGSGDRYFQEDQKKLVPEGIEGMVPHKGPLGDLVFQLVGGLRAGMGYTGSAT
                     IDELRTRARFMKISGAGLIESHPHDVQITKEAPNYERR"
     gene            complement(2203..2925)
                     /gene="recO"
                     /locus_tag="VFD74_03515"
     CDS             complement(2203..2925)
                     /gene="recO"
                     /locus_tag="VFD74_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZK48651.1"
                     /translation="MRLDRYLKTEALVMGSMRLREADRIVTLYTRERGRLSAVAKGVR
                     RTKSRIGGRLEPFSLVHVVLYSGSGSLYTITGVETVRSFQRVRDSLERMEEGGRLLEA
                     VRRLFPEEEGNPASFNLLVRGVARLAAAEDRPSAALAVLATRLKLLVALGYLPELDSC
                     VQCGSEEMLCGFRPSLGGMVCSDCYPQEHHDCFAISPDGLAALHYLLDNPLSKLDHSE
                     LDGFAAAEVERAVSQTLAHHGH"
     gene            complement(2971..3666)
                     /gene="deoC"
                     /locus_tag="VFD74_03520"
     CDS             complement(2971..3666)
                     /gene="deoC"
                     /locus_tag="VFD74_03520"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZK48652.1"
                     /translation="MRVEELAKTIDTTLLDPTSTQADIEKLCRDAAEHHFASVCVFPY
                     FVPYAKKVLRGQDVKVGTVISFPYGADTQRGKILAAENAVAAGADELDVVMNIPAMLS
                     GDFRYVRDELSSLVRSVRMKSVNAGRGLVLVKVIVEAYYLDEKLKRLVCKIVEDSGAD
                     FIKTSTGKAPGGATAKDVELFRDLLSENVGVKAAGGIRTAEDAEAMVNAGAARIGTSH
                     GVSIIESFGNEKE"
     gene            complement(3990..4994)
                     /gene="era"
                     /locus_tag="VFD74_03525"
     CDS             complement(3990..4994)
                     /gene="era"
                     /locus_tag="VFD74_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZK48653.1"
                     /translation="MLPDDPRSDVDEPATHPDVGRPATHPDVDQPAGSGFLDTPPGFR
                     SGFVALVGRPNVGKSTLVNKVLGRKVSITASKPQTTRHRVAGVHSAPGYQLILVDMPG
                     FQKPMDVLTRRMQELVDATISEVDAVLFVLDAGERVGAGDRFISRVLAEATTPVVVAL
                     NKIDLLDDGRKLTLAAEAESLVPGAVVVPVSALDGTGVGPLVERLAALLPEGPRYFPP
                     DAVTDQPEETLVAEFIREKLIAATEEEIPHSIAVQVLEMEPREESDLLYIRAAVYVER
                     DSQRPIVLGKGGAVIKQVGIEARRDIEWLLGERVYLDLVVKVRKHWRKSSRMLDDLGL
                     "
     gene            complement(4984..5475)
                     /locus_tag="VFD74_03530"
     CDS             complement(4984..5475)
                     /locus_tag="VFD74_03530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZK48654.1"
                     /translation="MGTLELVRLERPTTSELAELAVYDQEAFGVTGLRSYDLGVVARA
                     GGLYVGLVDGTVAASCQLMRMMDEPGMFWVLGFYVRPDFQRQGLGSELLCAVGAEVLR
                     RDGSGLMLTASPDSTVALNLYLGFGFKIVEEVADFYGPGEDRFVLRWDAGATSTERDC
                     HVA"
     gene            complement(5475..5903)
                     /locus_tag="VFD74_03535"
     CDS             complement(5475..5903)
                     /locus_tag="VFD74_03535"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZK48655.1"
                     /translation="MDLSPLAEPAPTEQYRDLIEAARGAAARAYAPYSHFRVGAAVLG
                     GSGRVYTGCNVENSSYSVTLCAERNAVGTAVAAGETTLVAVAVASPGPDPDDLTPCGM
                     CRQFLAEFGEGIVVVCRQGGAWRATELRRLLPGAFVLGAG"
     gene            complement(5914..6639)
                     /locus_tag="VFD74_03540"
     CDS             complement(5914..6639)
                     /locus_tag="VFD74_03540"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZK48656.1"
                     /translation="MSREGRRRSTALESFNYAFQGLIYVFRHQRNMRVHVIIAIVVLL
                     GSLFLDLTRLELVAVLFAITLVFMAEMLNTAIEAAVDLVTSSFDPKAKIAKDVAAGAV
                     LAAAINSLIVAYFVFADKATHPAATMLANVRRSPAHITFIAFLVTVLLVVITKAVFGR
                     GLPLSGGLPSGHAALAFSGWTAVTYLVSDHPYGILASAVAMIMAVLTAQTRVEAGIHS
                     TLEVVLGAILGILITTIAFQLWF"
     gene            complement(6645..7124)
                     /gene="ybeY"
                     /locus_tag="VFD74_03545"
     CDS             complement(6645..7124)
                     /gene="ybeY"
                     /locus_tag="VFD74_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZK48657.1"
                     /translation="MFLVDVVNETDLPIDEAAAVRAVLFVFESEGLSPGEVALAFIDE
                     DAMAELNERYRGGDGATDVLSFPGDDDSDWPDPEADDDESRPPSLGDVIVCPSVARRN
                     AAEDGITTAEELRRLVVHGVLHLLGYDHELDDGEMKAREAAILDDPAWGASALLPSE"
     gene            complement(7144..9528)
                     /locus_tag="VFD74_03550"
     CDS             complement(7144..9528)
                     /locus_tag="VFD74_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HZK48658.1"
                     /translation="MSVRHTVNERLAGVRDHIDSRDGRRFGMGLTVFLAVVLTLVISS
                     NYLPSRHHFQAGDVAGEEVVATRTVIFENRAATEELRKQVASLVQPAYSVNPTALSSV
                     VADVGGFFDQAREAKRSLAPSTTLPPPSTAAGSTGSTEGGGTTVSTEPLVNLGLARAR
                     LREVVPSTISEGTLALVVAAPNETLLRLRADVSSVLRFLYAGSVTQTALETARQKLRG
                     QAETVSGDEEYRVAVYEIAAAYLRPNQVYDEEATRARRDQAMQEVAPVTVSVLKGERI
                     VQPGDTITEQTMLALSALGLMGRPLGWETWLGSFIIVVLQLLVLSGLLSRTNRPVLAD
                     NTLMLALATLIILFTLVARIMVVPPLSPYLVPLAALAMITSIITKPRTALLLVSLQAL
                     NVGLMTGMDFGFVLVALITAIFSLYPVSHLAQRIELLAAGALVAAVAGATVFAVELLR
                     EAPILDALQASLWGLGNGALSMVLTVSILMIYEPLFNLTTPLRLLELANPSQPLLRRL
                     MQVAPGTYNHSILMGNLAEAAAEAIGADPLLARVGAYYHDIGKTLRPEYFIENQLHVS
                     NPHDRLNPNLSKLAITAHVKDGEDLARQYGLPKPVIDIIRQHHGTSVLSYFYHKAQET
                     TGGDVPEETYRYEEEKPASPEAAIIMLADSVEAAAKAMKAPTVKKMQGLIRTILKQKM
                     DDGQLDKSQLTFGDLEKIRLVFESGLRGLAGHRIEYPRERENGKQERRPPGGGADAAA
                     RAAAGPKRKPRQRPATGATASPAPEAPEAPAREPAPPESPAPESPVGAPESEAE"
     gene            complement(9579..10589)
                     /locus_tag="VFD74_03555"
     CDS             complement(9579..10589)
                     /locus_tag="VFD74_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZK48659.1"
                     /translation="MTKRILDLENDLAVLLVGEHDANLRRLEHLLDCDLSLRGNQLSI
                     EGSEAEVERVSELVLELLALLESGHTLDKATLELAASLGMRDNGARSRLTTIVGDVIL
                     EHRGHRIAPKTVNQKAYVDAIRMNTITFGIGPAGTGKTYLAMAMAAASLLSGDVARII
                     LTRPAVEAGEKLGFLPGGLMDKVDPYLRPLFDALYDMMDPDTLAGHLDRGSIEVAPLA
                     YMRGRTLNDAFIILDEAQNTTPQQMQMFLTRLGFGSRMVVTGDVTQIDLPSGQYSGLA
                     TVREILGDIRDIGFVTFDGQDVVRHVLVQDIVNAYKTYVERKRGDGSRSHRESTDRRH
                     TP"
     gene            complement(10708..11148)
                     /locus_tag="VFD74_03560"
     CDS             complement(10708..11148)
                     /locus_tag="VFD74_03560"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZK48660.1"
                     /translation="MLESLQSRVNQGMKAGRKQEVRVLRMLVSELKRSSKDLGRELDS
                     AEELKVLHKERKRRIESIEAFKAGGRDDLVADEEFALVLVGELLPRQLEGVELIALVD
                     RCIANVGAGSIKDMGAVMSRLKQEAGPQVDGAAASRIVKERLSG"
     gene            complement(11151..11492)
                     /locus_tag="VFD74_03565"
     CDS             complement(11151..11492)
                     /locus_tag="VFD74_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZK48661.1"
                     /translation="MGHCIFCKIIGGEIPGDVVLEDEEFFAIRDISPKAAQHVLVMPR
                     EHVESLNEVGVWTDGRGQRLLAFTARVAADLGIARSGYRVITNVGPDGGQEVDHLHFH
                     VLGGERLGDLV"
     gene            complement(11507..12283)
                     /locus_tag="VFD74_03570"
     CDS             complement(11507..12283)
                     /locus_tag="VFD74_03570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HZK48662.1"
                     /translation="MRPRFFVDPASVPPLGSLLELSSTDARHARTVLRVRPGEPCELV
                     LVGARAVAEAAFEEVGERVTARLTSWVAEASAPRVELLLVQGLPQPRKVDEIVEKGTE
                     VGIDSFLIVPAVGSPRVPAERVAARLARWRTVAREAAKQSRQLAIPEVRTAGSLQEAL
                     ALVGSEGWRSLLLTPGAGEYLGSVLAGDTRTGTHGIPPAGPPVRWALWVGPEGGWSSA
                     EVEVLTSAGCLPATLGSRVLRTETAGTVAGALARYALSDW"
BASE COUNT         2174 a         4299 c         4031 g         2003 t
ORIGIN      
        1 ctcttcgcgc tcggcctacc tgcccctgac ggcgcgcacc atgtacacgg tgatcgtccc
       61 gaagagggcg gcaaggtaga agagcaccga gaaggtaccc agcccatcgg catccccacg
      121 ggcgactctg acggtccccc acgtcaaggt cgcaaacatc cacaccatct ccagcttgag
      181 cagggtgatg agatcgaccg ccagacgata ctgacggccg gcgttttcct ccgtgatgcg
      241 caccgggtag ttgtagatgt gcggatagcg ctccaggatg gtcagccctg cgtacatgac
      301 cacccccacc acgggcatca ccagcagggc ccagcgaccg ccccatccgt cgggcgtccc
      361 gtcgcctccg aagtgcgtgg ggatgcgcgc ggggagtgac gtccacgccg cgcccgcgat
      421 caccaccgtc gcgatcgtgc ccgtcaacgc aagcgcctct atcaccttcc acatcccgcc
      481 gcgttcggta ggcacccgcg gccgtcgcat cagtcccacc cggtcacctc gctgtgagtc
      541 gtattccgtc cgaatctgat tctacacgtg ccgtcggcac cgctttccgc tagaatcgtt
      601 gaaacggaag gcgggaggtc ggcgtgatcg attgggtgcg gaagttcgag gcggagggcc
      661 tgtcttttga cgacgtgctc ctgatcccgg cggagtcgcg gatccttccc aagggcacgg
      721 atgtccgcac caggctcacc aagacaatcg agctgaacat cccgctgctc agtgccgcga
      781 tggacaccgt ctccgagacc gccctcgcga tcgccttggc ccgcgaaggc ggcctcacga
      841 tcatccacaa gaaccaatcc atcgaacgcc aagccgacat gatccgcaag gtgaaacgga
      901 gcgaggcggg gatgatcgtc gatcccatca ccctgcctcc taccgccacc tacggcgacg
      961 ccgaagcgct catggctgag tacaagatct ccggagtgcc catcgtgcac cccgacggca
     1021 cgctggccgg catcgtcacc aaccgtgaca cccggttcga gcacgacctc tcgcggccca
     1081 tcagggagct gatgacctcc gagaacctgg tgacggtccc tgtgggcacc accctcgacc
     1141 aagccctcca tcaattcaag gtccacaaga tcgagaagct cctggttgtg gacgaccagt
     1201 tcaaactccg cggactcatc accatcaaag acatcatgaa gaagatcgag ttccccagcg
     1261 cctgcaagga cgagttgggc cgtctgcgcg taggcgcagc ggtgggcgtg gccaaggaca
     1321 ccgacgcccg catggaagcg ctcgtcgagg cccaggtcga cgtcgcctgc atcgacacgg
     1381 cgcacggcca ctcgcgaggc gtgctcgaca ccatcctgcg ggtcagggag aactatcccg
     1441 acgtgcaact catcgccggc aacgtgggca ccgccgccgg cgcccaagcg ctcatcgatc
     1501 gcgggatcga cggcatcaag gtgggcatcg ggccgggctc catctgcacc acgcgggtgg
     1561 tcaccggctg cggcgtgccc cagatcacag ccgtatcgga gtgcgcccgc gtggccgacg
     1621 aggcggggat accgatcatc gccgacggcg gtgtccgcta ctccggcgac ctggtcaagg
     1681 cgctcgccgc gggcgccaac agcgtcatga tcggctccct gttcgccggc accaccgaga
     1741 gcccgggcga acgcatcctc tgggagggcc gcagctacaa ggtctaccgc gccatgggct
     1801 cggtgagcgc catgagcgaa ggcagcggcg atcggtactt ccaggaggac cagaagaagc
     1861 tggttcccga ggggatagaa gggatggttc ctcacaaggg gcctctgggc gacttggtgt
     1921 tccagctggt cggcggcctc cgcgccggta tgggctacac gggctcggct accatcgacg
     1981 agttgcgcac ccgcgcccgc ttcatgaaga tctccggagc aggcctcatc gagagccacc
     2041 cgcacgacgt gcagatcacc aaagaggcgc ccaactacga gcgacgctag ccgaagaggt
     2101 ccgggatccg ggatccggga tccgggatcc ggggtccggg gtccggggtc aggcgcccga
     2161 gggccaggtg cctacgccca gcccgaacgg ccgtggaccc ggtcagtgac cgtggtgggc
     2221 cagggtctgc gagacggccc gctccacctc ggcggccgcg aacccatcca actcggagtg
     2281 gtcgagcttc gacagcgggt tgtcgagcag gtagtgaagc gccgcgaggc cgtcgggact
     2341 gatggcgaag cagtcgtgat gctcctgggg gtagcagtcg ctgcacacca ttccgcccag
     2401 cgacggtcgg aagccgcaca gcatctcttc gctcccgcac tgcacgcagg agtcgagttc
     2461 ggggaggtac cccagcgcca cgaggagctt gagcctggtg gccaggaccg cgagtgcggc
     2521 tgagggtcga tcctcggcgg cggccaaccg agccaccccg cgcaccagca ggttgaacga
     2581 cgcgggattc ccctcctcct cagggaacag ccgtcgcacc gcctcgagca gacgcccgcc
     2641 ttcctccatg cgctcgagag agtcgcgcac gcgctggaag cttcggaccg tctcgacccc
     2701 cgtgatcgtg tagaggctgc cgctacctga gtagagcacg acgtgcacca ggctgaaggg
     2761 ctccaaccga ccgccgatgc gcgacttggt gcgacgcaca cccttcgcaa ccgcagagag
     2821 tcggccacgt tcgcgcgtgt agagagtgac gatccggtcg gcctcgcgca atcgcattga
     2881 gcccatgacc agggcctcgg tcttgaggta gcggtcgagc ctcacttcac gagtcccctg
     2941 acgatgcgcc ccgagcggga cgcgctgacg ctactccttc tcgttgccga acgattcgat
     3001 gatcgacacc ccgtgcgacg tgccgatgcg ggccgctccg gcgttgacca tggcctcggc
     3061 atcctccgcc gtgcggatgc cccccgccgc tttgaccccg acgttctccg acagcaggtc
     3121 gcggaacagc tctacgtcct tcgcagtcgc tccgcccggc gccttgcccg tggaagtctt
     3181 gatgaagtcc gcaccggagt cctccacgat cttacacacc agacgcttga gcttctcgtc
     3241 gaggtagtag gcttccacga tcaccttcac gagcacgagt ccccggccgg cgttgacgct
     3301 cttcatgcgc accgacctca ccaggctcga gagctcgtcg cgcacgtagc ggaaatctcc
     3361 ggacagcata gcggggatgt tcatgaccac atcgagttcg tcggcgccgg cggcgaccgc
     3421 gttctcggcc gccagtatct tgccccgctg agtgtcggct ccgtacggga aggagatcac
     3481 ggtgccgacc ttcacgtcct ggcctcgcaa gaccttcttg gcgtagggga cgaaataggg
     3541 gaacacacac acggaggcga aatgatgctc ggcggcgtcc cggcacagct tctcgatgtc
     3601 cgcctgcgtg gaggtgggat cgagcaacgt ggtgtcgatg gtctttgcga gttcctctac
     3661 tctcaagtct cctcctcatg ggccgccggt cgagtcgagg ggcccttcac ggtgaagttc
     3721 tatgatacac ctgggtacaa gggctccggc cgcacgggcg cccgagacgg ccgcggcggg
     3781 ccgacccgcc gcgccgcggg accgcgggcc tccggatgcc tcccccagta ggctgctgaa
     3841 aagcgaagcc ttggccccca gaacgcactg ggcggcgcga tacggcgtcc tcggtcgccc
     3901 acgtagcgcc gctacgagcg cttcctgcgt ccttgtctcg cactcgccca tcgcgctctg
     3961 aaggcgaagc gtcactcttc agcagccttc tagagaccca ggtcgtcgag catgcgcgag
     4021 ctcttgcgcc agtgcttcct caccttgacc accaggtcca ggtacaccct ctcgcccagc
     4081 agccactcga tgtcgcgacg ggcctcgatg cccacctgct tgatcacggc gccacccttg
     4141 ccgagtacga tggggcgttg gctgtcccgc tccacataca ccgcggcccg gatgtagagc
     4201 aggtcgctct cctcgcgtgg ctccatttcg agcacctgca ccgctatcga gtgcgggatc
     4261 tcctcctccg tcgcggcgat gagcttctcg cggatgaact ccgccacgag tgtctcctcg
     4321 ggctggtcgg tgacggcgtc cggcgggaag tagcgaggcc cctcgggcag gagcgccgcc
     4381 agtcgctcca cgagcgggcc gacgccggtg ccgtcgaggg cactcaccgg caccacgacg
     4441 gcgccgggga cgaggctttc ggcttcggca gccagggtca gcttccgccc atcgtcgagc
     4501 agatcgatct tgttcagcgc caccaccacc ggcgtggtgg cctcggccaa aacgcggctg
     4561 atgaagcggt ctcccgcacc cacccgctcg cccgcgtcca agacgaacaa cacggcgtcc
     4621 acctcggaga tggtcgcgtc cacgagttcc tgcatccgtc gagtgagcac atccatcggc
     4681 ttctggaagc cgggcatgtc caccaggatc agttggtaac cgggggccga gtgcacgccg
     4741 gccacccggt ggcgcgtggt ctgcggcttg ctggccgtga tgctcacctt gcggccgagc
     4801 accttgttga cgagtgtgga cttgcccacg ttggggcgcc caaccagggc cacgaacccc
     4861 gaccggaagc cgggcggggt gtccaggaag ccggaaccgg cgggttggtc tacgtccggg
     4921 tgcgtggcgg gtcggcctac atccgggtgc gtggcgggtt cgtctacgtc tgagcgcggg
     4981 tcgtcaggca acatggcagt cccgttcagt cgacgtggca ccggcatccc agcgaagcac
     5041 gaaccgatcc tctccggggc cgtagaagtc ggccacctcc tctacgatct tgaatccgaa
     5101 gcccaggtag aggttgagcg cgaccgtgct gtcggggctg gcggtcagca tcagtccgga
     5161 gccgtcacgc ctcaggacct cggcgcccac ggcgcacagg agttcgctgc caaggccctg
     5221 cctctggaag tcaggcctca cgtagaatcc cagcacccag aacatccccg gctcgtccat
     5281 catccgcatc agctgacacg aagcagccac cgtgccgtcg accagaccca catagaggcc
     5341 gcccgcccgg gcgaccacgc ccaggtcgta gctgcgcaac cccgtcaccc caaaggcttc
     5401 ctgatcgtag accgcgagtt cggcgagttc gctcgtcgtg ggacgctcca gcctgaccaa
     5461 ctcgagcgtt cccactagcc ggcccccagc acgaatgccc cgggaagcag ccgccgcagc
     5521 tccgtcgccc tccacgcgcc gccctgtctg cagacgacca cgatcccctc accgaactcg
     5581 gccaggaact gccggcacat gccgcaggga gtcaggtcat cggggtcggg tcccggcgag
     5641 gcgacggcca ccgcgacgag ggtcgtctcg ccggccgcca cggccgtacc caccgcgtta
     5701 cgctcggcgc agagggtcac cgaatagctg gagttctcca cgttgcaccc cgtgtacacc
     5761 cggccggagc cacccaagac agcggccccc acacggaagt gcgaataggg agcgtacgca
     5821 cgcgccgcgg cgccgcgggc ggcctcgatc aggtcgcggt actgctcggt aggcgcgggc
     5881 tcggcgagcg gggataggtc caagatgatc ctttcagaac cagagttgaa aagcgatggt
     5941 agtgatcagg atacctagta tcgcacccag gaccacctcg agggtgctgt ggatgccggc
     6001 ctcgacccgc gtctgggcgg tgagcacggc catgatcatg gccacggcgc tcgccaggat
     6061 cccgtagggg tggtccgaga ccaggtaggt gacagccgtc cagcccgaaa aagccagggc
     6121 ggcgtgaccg gaaggcagcc cgccggacag cggaaggccc cgcccgaaca cggccttcgt
     6181 gatcaccacc aggagcaccg tcacgaggaa cgctatgaac gtgatgtgcg ccggcgaacg
     6241 ccgcacattc gcaagcatgg tggcggccgg atgggtggcc ttgtcggcga agacaaagta
     6301 ggcgacgatc agcgagttga tggccgccgc caggaccgcg cccgccgcca cgtccttggc
     6361 tatcttggcc ttgggatcga aggagctcgt gacgaggtcg accgccgcct cgatggccgt
     6421 attcagcatc tctgccatga acaccagggt tatggcgaag agcacagcga caagctccag
     6481 gcgcgtcaga tccaagaaga ggctgcccaa caggacgacg atggcgataa tcacgtgcac
     6541 ccgcatgttg cgctggtgcc ggaacacgta gatcaaaccc tggaaagcgt agttgaagct
     6601 ctcgagcgcg gtggagcgac ggcgaccttc ccggctcact ggcactactc cgacggcaag
     6661 agtgcagagg ctccccacgc gggatcgtcc aggatcgccg cctcgcgcgc cttcatctca
     6721 ccgtcatcga gctcatggtc gtagccgagc agatggagca caccgtggac caccaagcgg
     6781 cgcaactcct cggccgtggt gatgccgtcc tcggcggcgt tgcgacgggc cacggaaggg
     6841 cacacgatca cgtcgcccag ggacggcgga cggctttcgt cgtcgtccgc ttcggggtcg
     6901 ggccaatcgg agtcgtcgtc gccgggaaag gagagcacgt ccgtggctcc gtcccctcct
     6961 cggtagcgct cgttgagctc ggccatggcg tcctcgtcga tgaaggccag cgcgacctct
     7021 ccagggctca acccttcgct ctcaaatacg aagagcacgg cgcgcacagc ggcggcttcg
     7081 tcgatgggca ggtccgtctc gttgacgacg tcgaccagga acatcaggcg acagctccca
     7141 cgactattcg gcctcggatt cgggtgcgcc cacaggcgac tcgggtgcag gcgactcggg
     7201 gggcgcgggc tcgcgcgcgg gcgcctccgg cgcctccggc gcaggcgacg cggtagcccc
     7261 ggtggccgga cgctgtcgag gtttccgctt cgggccggcg gccgctcgcg ccgcggcatc
     7321 cgccccgccg cccggagggc gacgctcctg cttgccgttc tcccgttccc gcggatactc
     7381 gatccggtgg ccggcgagtc cacgcaaacc gctctcgaag accaatctga tcttctcgag
     7441 atcgccaaag gtgagctgcg acttgtccag ctgtccgtcg tccatcttct gcttgaggat
     7501 cgtcctgatc agcccctgca tcttcttcac ggtcggggcc ttcatagcct tggccgcggc
     7561 ctccacgctg tcggccagca tgatgatggc ggcctcaggt gaggcgggtt tctcttcttc
     7621 gtagcggtag gtctcctcgg gcacgtcgcc gccggtggtc tcttgggcct tgtgatagaa
     7681 gtaggagagc accgaggtgc cgtggtgctg gcggatgatg tcgatgaccg gcttgggcag
     7741 accatactgc cgagccaaat cctccccgtc cttcacgtgg gccgtgatgg cgagtttcga
     7801 caggttcggg ttgagcctgt cgtgggggtt cgacacgtgc agctggttct cgatgaagta
     7861 ctccggacgg agcgtcttgc cgatgtcgtg gtagtaggcg ccgacgcgcg ccaggagggg
     7921 atcggcgccg atggcctcag cggcggcctc ggcgagattc cccatgagga tgctgtggtt
     7981 gtaggtgcct ggggccactt gcatcagccg ccgcagcaac ggttgcgagg ggttggccaa
     8041 ctcgagtagc cgcagcggcg tggtcaggtt gaacagtggc tcgtagatca tgaggatgct
     8101 gaccgtgagc accatgctga gcgccccgtt ccccaggccc cacaggctgg cctgcagggc
     8161 atcgaggatc ggcgcctcac gcagcagctc cacagcaaac acagtcgcgc ccgccacggc
     8221 ggcgaccaga gcccccgctg ccagcagttc gatgcgctgg gccaggtgcg acacggggta
     8281 gagcgagaag atggcggtga tgagcgccac caggacgaac ccgaagtcca tgccggtcat
     8341 gaggccgacg ttgagcgcct gcagcgacac gagcagcaag gcggtgcgcg gtttcgtgat
     8401 gatcgacgtg atcatggcta gcgccgctag gggtaccaga tagggggaca gcggcggcac
     8461 gaccatgatc cgggccacca gcgtgaacag aatgatcagg gtggctaggg ccagcatgag
     8521 agtgttgtcc gccagcacag gccggttcgt gcgagagagc agaccgctca gcaccaggag
     8581 ctgcagcacc acgatgatga aggaacccag ccaggtctcc cagcccagcg gcctccccat
     8641 gagtccgagc gcgctcaagg ccagcatggt ctgctcggtg atcgtgtccc cgggctgcac
     8701 gatccgctcg cccttcaaca ccgacaccgt gaccggggcg acttcctgca tggcctggtc
     8761 acgacgagcc ctggtggcct cctcgtcgta cacctggttc gggcgtaggt aggccgccgc
     8821 gatctcgtac acggcgaccc gatactcctc gtcgcccgac acggtctccg cctgaccacg
     8881 cagtttctgc cgagccgtct cgagggccgt ctgcgtgacg ctgcccgcat acaagaagcg
     8941 cagcacgctc gacacgtcgg cgcgcagacg aagcagcgtc tcgttgggtg ccgcgaccac
     9001 cagggccaac gtgccttccg agatggtgga cggcaccacc tcacgcaggc gggcgcgggc
     9061 caatccgaga ttgaccagag gctcggtgga tacggtcgtc ccgccgccct ccgtcgaccc
     9121 ggtgctgccg gcagcggtgg atggcggcgg cagcgtggtc gaaggcgcga gagatcgctt
     9181 cgcctcccgc gcctgatcga agaagccgcc tacgtcggcg acgaccgacg acagggctgt
     9241 gggattgacg ctgtaggcgg gctgcaccaa ggacgccacc tgcttgcgca actcctcagt
     9301 cgccgcccgg ttctcgaaga tgacggtgcg ggtggcgacc acttcctccc cggccacgtc
     9361 accggcctgg aagtgatggc gagagggcag gtagttcgac gagatgacga gcgtgagcac
     9421 cacggcgagg aacaccgtga ggcccatccc gaagcggcgg ccgtcgcggg aatcgatgtg
     9481 atcccgcacg ccggcgagac gttcgtttac cgtatgtcgc acgctcatgg atgactcaca
     9541 agtgaagggc cctcgtctgg atcggggaag ggaggtggct agggggtgtg acgccggtcg
     9601 gtggattcgc ggtgagatcg actcccgtca ccacgcttcc gctccacata tgttttgtac
     9661 gcgttgacga tgtcctgcac gagcacatgc cgcacgacgt cttgaccgtc gaaggtgacg
     9721 aagccgatat cgcggatatc gcccaggatc tcgcgcaccg tcgccaggcc cgagtactga
     9781 ccgctcggca ggtcgatctg cgtgacgtcc cccgtcacga ccatgcgcga tccgaatccc
     9841 agacgggtga ggaacatctg catctgctgg ggcgtggtgt tctgagcctc gtccaggatg
     9901 atgaaagcgt cgttgagtgt gcgccccctc atgtaggcca atggagccac ctcgatggac
     9961 ccacgatcca ggtggcccgc cagggtgtcc ggatccatca tgtcgtacag cgcgtcgaac
    10021 agcgggcgaa ggtaggggtc gaccttgtcc atgagaccac ccggcaggaa ccccagcttc
    10081 tcacccgcct ccaccgccgg ccgagtgagg atgatgcggg ccacgtcccc cgagagaagc
    10141 gaagccgccg ccatggccat ggcgaggtag gtcttgcccg tgccggcggg accgatgcca
    10201 aaggtgatgg tgttcatgcg gatagcatcg acgtaggcct tctggttgac cgtcttcggc
    10261 gcgatgcggt ggccgcggtg ctccaatatg acgtcgccca cgatggtggt gagcctgctc
    10321 ctggccccgt tgtcgcgcat cccgagcgaa gccgcgagtt ccagggtcgc cttgtcgagg
    10381 gtatgaccag attcgagcag ggccaacagc tcgagcacga gctccgacac ccgctccacc
    10441 tccgcctccg acccttcaat ggagagctgg ttgccgcgca gcgagagatc gcagtcgagc
    10501 aggtgttcga gccggcgaag gttcgcgtcg tgttcgccca cgagcaacac ggccagatcg
    10561 ttctcaagat cgaggatcct cttggtcacc gcggtgcgtc tccgatgttc ggtcggcacg
    10621 ccctccggcg gcgtgcgcag agatccatcc cgccacgatc tcgcaccgcc cacggcgcgg
    10681 gtctgcgaag tgctggagtg ccgcccgtca gccagacagc cgctccttga cgatgcgcga
    10741 tgccgccgca ccatccacct gcggcccggc ctcctgcttc aggcggctca tgaccgcacc
    10801 catgtccttg atcgacccgg cgcccacgtt ggcgatgcag cgatccacca gagcgatcag
    10861 ttccactccc tcgagctgcc ggggaagcaa ctcccctacg agaaccagag cgaactcctc
    10921 gtcggcaact aggtcgtcgc gacctccggc cttgaacgcc tcgatcgact cgatgcgccg
    10981 cttgcgctcc ttgtgcagca ccttgagctc ttcggcggag tcgagttccc tgcctaggtc
    11041 tttggacgag cgcttgagtt cggacacgag catgcgaagc acgcgcactt cctgcttgcg
    11101 accggccttc ataccttggt tcacgcgcga ttgcagggat tcaagcacgc tcaaaccaga
    11161 tcgccgagcc tctccccacc gagcacgtgg aagtgcaggt gatcgacctc ctggccgccg
    11221 tcgggaccga cgttggttat cactcggtat ccggatcggg cgatgcccag gtcggccgcg
    11281 acgcgggccg tgaaagccag cagccgctgg ccccgcccgt cggtccacac gcccacctcg
    11341 ttgagagact cgacgtgctc ccggggcatg accagaacgt gctgagccgc ctttggggat
    11401 atgtccctga tagcgaagaa ctcctcgtct tcgaggacca cgtcgccggg gatctcacca
    11461 ccgattatct tgcagaagat gcagtgtccc atgacccatg attatactac caatccgata
    11521 gggcataccg ggccagggct cccgccaccg tcccggccgt ctcggttcga agcacccgcg
    11581 agccgagggt cgcgggcagg cagcccgcac tcgtgagaac ttccacctcc gccgaggacc
    11641 agcccccttc gggccccacc cacaacgccc acctcaccgg cgggccggcc ggggggatgc
    11701 cgtgggtgcc cgtgcgcgtg tctcccgcca acaccgaccc gagatattct cccgccccgg
    11761 gggtcaagag cagcgagcgc cacccttccg agccgaccaa ggccagggcc tcctgcagcg
    11821 agccggctgt gcgcacctcg gggatcgcga gttgtcggct ctgcttggcc gcctccctcg
    11881 ccacggtgcg ccacctcgcc agccgtgccg ccacgcgttc cgccggcacc cgcggcgaac
    11941 cgaccgctgg gacgatgagg aacgagtcga tacccacctc ggtgcccttc tccacgatct
    12001 cgtccacctt ccgaggctgc gggaggccct gcaccaggag cagttccacc cggggggccg
    12061 aggcctccgc gacccaggac gtcagacggg cggtcactcg ctctcccacc tcctcgaacg
    12121 cggcctcggc cacagccctc gccccgacga gcaccagttc gcagggttct ccaggacgaa
    12181 cgcgcaggac agtccgcgcg tggcgggcgt cggtggagga gagttcgaga agcgagccga
    12241 gcggaggaac gctcgccggg tctacgaaga agcgcggtcg cacgatccac cgtcgcgggg
    12301 tccgcgaccg cacggtccgc ggccggaggg tccaccgtcg cggcctgcgc ggccggagaa
    12361 tgcgcagccg aaaggcgcac agccgcgcga tccgctgtca ctcacccgcg gtagcaagcg
    12421 agagggcctt ctgcagcgtc tcgtcgacgc cactcgtgga tggatcgtcg ggcatcacga
    12481 cgtcaggagc gatgcccttc ttgttga
//
LOCUS       DASRMJ010000124         6031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_125237, whole genome shotgun sequence.
ACCESSION   DASRMJ010000124 DASRMJ010000000
VERSION     DASRMJ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125237"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFD74_03575"
     CDS             complement(<1..230)
                     /locus_tag="VFD74_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZK48663.1"
                     /translation="MFRRVLVANRGEIAVRVVRACHELGIEAVAVYSTADESCAHVRA
                     ADQAVRLGPPDPRRSYLNIPSLIAAAKTTGCEA"
     gene            complement(240..2147)
                     /gene="accB"
                     /locus_tag="VFD74_03580"
     CDS             complement(240..2147)
                     /gene="accB"
                     /locus_tag="VFD74_03580"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HZK48664.1"
                     /translation="MRARRIGITDTSLRDAHQSLWATRMRVGHMVPVLRRMDQIGFHS
                     LEVWGGATFDACLRFLDEDPWERLRTIKHHCRKTPLQMLLRGQNLVGYRHYGDDVVRE
                     FVRLAADSGVDIFRVFDALNDTRNFHTAVDAIKESGKHFQGAVVFTISPVHTLDHYVE
                     VAQELADLGADSICIKDMAALLSPYFAERLVTRLKREIDLPLHLHCHYIGGLAPMTYL
                     KAIEAGVDVVDTATVPVAFGASQPATEMLVTALKGTPYDTELDLDALFDMAEYFEGVR
                     ESGGFERGVTSLTHMRVYSHQVPGGMISNLISQLKEQKAEDRLQEVLEEIPKVRAEVG
                     YPPLVTPMSQIVGTQAVLNILIGKRWQVVPDEMKAYLRGRYGTAPGPIDAKILKSVLG
                     DEQPISVRPADLIEETVEQYREEVGSLARSEEDVLSYALFPNAARNYFERRHHGAEQD
                     VFLTGEPEPASTEVQAPALAGTDRVRDLIKAVEASDVEEVTVEDAGMRITIRRGGRAL
                     SDSGFVQAPFQGAAAAPPAGVRQAGADAAVPAGGAADAPRATADDSLFRVRAPMVGTF
                     YAASSPTGPPFVDVGSRVQVGDVLCILEAMKLMNEVASEVSGVVKAVLVGDAQPVEYD
                     QPLFAVELDQE"
     gene            complement(2182..2739)
                     /gene="efp"
                     /locus_tag="VFD74_03585"
     CDS             complement(2182..2739)
                     /gene="efp"
                     /locus_tag="VFD74_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZK48665.1"
                     /translation="MISTNQFRTGMTIRFEDENYRIVDFQHVKPGKGGAFVRTKLKSL
                     ATGRVLDKTFRPEQKFDQVRVESRSMTYLYDEPDQVVFMDASTYEQLALPKSVLEGRF
                     DLMTVNMEVEVLFVDEQASDVELPTWVDLAVAETAPGVRGDTVSGGSKTAVLETGATL
                     QVPLFIETGDVIRVDTRTREYNTRV"
     gene            complement(2884..4008)
                     /locus_tag="VFD74_03590"
     CDS             complement(2884..4008)
                     /locus_tag="VFD74_03590"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HZK48666.1"
                     /translation="MSRKARGVVEVVDDAGDRPYDDRLETLRSSLGLDGLSGLLVFDP
                     VNIRYLTGFTGSAGVVLVRPDECLLVSDFRYRVQAREQAPVARFVELEGAFSPLAPEL
                     VEGFEGCLGVEKDHLKVEEWEALAPALEGLDVSLVRGHVRKMRRVKSAGELRAVREAS
                     LLAVQVMERLSLMQVVGRTERDVALELEVWARKHGSEGIPFPYILAWGPNSAKPHAEP
                     GDTVIGQGGLLVVDLGAVVDGYASDITRTFSTGKVGPEEARAHRVTLEAQEAARREAG
                     PGIACRHVDKVARDVIEGEGLGDFFKHGLGHGVGLEVHEEPGLSSRSEDVLEPGMVVT
                     IEPGIYLEGVGGVRIEDSVAVTETGVEVLTEMDRGLLTLS"
     gene            complement(3968..>6031)
                     /gene="aroB"
                     /locus_tag="VFD74_03595"
     CDS             complement(3968..>6031)
                     /gene="aroB"
                     /locus_tag="VFD74_03595"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZK48667.1"
                     /translation="ASIESIFAERGEEGFRRVEERALAEVLDSAAEVAGGIVVALGGG
                     TVTWAESAERLAGGFVLLLSAQTEILWERVRHTGRPLAQSKEEFAALADARRASYLRT
                     ADVVLDVSGLDTEEAAQLVVAAVRQREAEVSGVSSRPTSGSLWKVEVGASPPLSTIVG
                     GWDSLDGLPSRVAGASRAGSRLFVVSDQAVWVHVGAEVLRALSSGDCAPAVYLLTPGE
                     ASKSIEVAVGCWEWLVDQGARRDDIVVAVGGGVVGDLAGFVAATYLRGVRFWQIPTTL
                     LAQVDSSVGGKVGVNLAGGKNLVGAFYQPELVVSDQSVLRSLPELDFASGLGEVVKYA
                     LLSGEPFLRELESGAADILARDPSRVSGIVERCVRSKAEIVVEDERDHGRRAVLNLGH
                     TVAHALEHAMGYGALSHGAAVGLGLLVVLAVSESSCGLNKEVLPRVRALLARFGLPTT
                     IARHARRCDGRCGPRQESERRRARSGVPAGRGRPGVGCPRRGRTTCGSIGGDPGMTHA
                     ATSKGDRPDDQPGGGLLVLLHGVNLGLLGERPAAHYGTVTLRDLETLVSAEAEEHGWR
                     CICHQTDDEGGFVRLIHQYRARAGAMLVNPGAWTHYSYAIRDALELVQAPIAEVHLSD
                     VTTRESWRRVSVISDVVSLTVSGEGPEGYRRAARALIASVGCSDGRGEGPDESQSERS
                     GGSGR"
BASE COUNT         1080 a         2208 c         1718 g         1025 t
ORIGIN      
        1 gcctcgcacc cagtggtctt ggccgcggcg atgagactcg ggatgttcag gtaactccgt
       61 cttggatcgg gcgggccaag ccggaccgcc tgatctgcgg cccgcacgtg cgcgcacgac
      121 tcatcggcgg tggagtagac agcgacggct tcgatgccga gctcgtggca ggcccgcacc
      181 acccgcacag cgatctctcc tctgttggca acgagtacgc gcctgaacac cccgggcacc
      241 tactcctggt cgagttcgac ggcgaagaga ggctggtcgt attcgaccgg ctgtgcgtcg
      301 cctaccagca ccgccttcac gacgccggag acctcggagg ccacctcgtt cataagcttc
      361 atcgcttcca ggatacacaa gacgtctccc acctggaccc gcgatcccac gtcgacgaac
      421 ggcgggccgg tgggtgacga agccgcgtag aacgtgccca ccatcggggc ccgcaccctg
      481 aacagagagt catcggccgt cgccctcggt gcgtcggcgg cgccccccgc gggcacggca
      541 gcgtctgcac ccgcctgccg cactcccgcc ggcggcgccg cggcggcacc ctggaacggc
      601 gcctgcacga agccggaatc gctgagcgcc cttccgcctc gacggatggt gatgcgcatg
      661 ccggcgtcct cgaccgtgac ttcctcaacg tccgaggcct cgaccgcctt aatgagatcc
      721 cgcactcgat cggtgccggc cagcgccggc gcctgcacct ccgtcgaagc gggttcgggc
      781 tcccctgtca agaacacgtc ttgctccgcc ccatggtgtc gccgctcgaa gtagtttctg
      841 gcggcgttgg gaaagagcgc ataactcaac acgtcctctt cgctgcgggc gagcgacccg
      901 acttcctccc ggtactgctc caccgtctct tcgatcaggt cggccgggcg cacgctgatg
      961 ggctgctcgt ctccgagtac gctcttcagg atcttcgcat cgatcggccc gggagccgtg
     1021 ccgtagcggc cgcggagata ggccttcatc tcgtcgggaa ccacctgcca ccgcttgccg
     1081 atgaggatgt tcaacaccgc ctgcgtaccc acgatctggc tcatgggcgt gaccagcggc
     1141 gggtagccga cctcggctct gaccttcggg atctcctcca acacctcttg gagcctgtct
     1201 tcggccttct gctctttcag ctgggagatc aagttggaga tcatgccccc cggaacctga
     1261 tgcgagtaca ctctcatgtg ggtgagcgag gtcacgcccc gctcgaaccc tccggattca
     1321 cgcactcctt cgaagtactc ggccatgtcg aagagggcat cgagatcgag ctccgtgtcg
     1381 tatggagtac ctttcaatgc ggtgaccaac atctccgtgg ccggttggct ggcgccgaat
     1441 gccaccggta ccgtcgccgt atccaccaca tcaaccccgg cctcgatggc cttcagatag
     1501 gtcatgggcg cgagaccgcc gatgtagtgg caatggaggt gcagaggaag gtcgatctcc
     1561 cgcttcaacc gggtgacgag gcgctccgcg aagtacggag acaggagagc ggccatgtcc
     1621 ttgatgcaga tgctatccgc ccctagatct gccaactcct gcgccacctc gacgtagtgg
     1681 tcaagcgtgt ggacggggct gatcgtgaac acgaccgcac cctggaagtg tttgccgctc
     1741 tccttgatcg cgtctactgc ggtatggaag ttgcgggtgt cgttcagcgc gtcgaacacc
     1801 cggaagatgt ccacgcccga gtccgctgca agcctcacga actcgcgcac cacgtcgtca
     1861 ccgtagtgcc ggtagcccac gaggttctga cccctcagaa gcatctggag aggagtcttc
     1921 ctacaatggt gctttatcgt gcgcaggcgt tcccacggat cctcgtcgag aaagcgtagg
     1981 caggcgtcaa acgtcgcccc gccccatacc tccagcgagt ggaacccgat ctggtccatc
     2041 ctccgcaaca cagggaccat gtgccccacg cgcatccgcg tggcccacag cgactggtgc
     2101 gcatcccgca ggctggtgtc cgttatccct attcgtcgtg ctctcattgg ttctccccgg
     2161 tgcgaaccgg caggctccga ctcagacccg ggtgttgtat tcgcgcgtgc gcgtatcgac
     2221 cctgatcacg tccccggtct cgatgaacag gggcacctgc aacgtggctc ctgtctcgag
     2281 gaccgcggtc ttgctgcccc cgcttaccgt atccccgcgc acccccgggg cggtctcggc
     2341 cacggctaga tcgacccacg tgggcagttc gacgtcggag gcctgttcat ccacgaacag
     2401 cacctctacc tccatgttga cggtcatgag atcgaagcgc ccctcgagaa ccgactttgg
     2461 aagcgccagc tgctcatagg tggaggcatc catgaagacc acttggtcgg gctcgtcgta
     2521 caagtaggtc atgctgcgac tttccacccg cacctgatcg aacttctgct ctggccggaa
     2581 cgtcttgtcg agaacgcggc cggtagccag gctcttcagt ttcgtgcgca cgaacgcgcc
     2641 gcccttgccg ggcttcacgt gctggaagtc aacgatgcgg tagttctcgt cctcgaatcg
     2701 aatagtcatg cccgtacgaa attgattggt tgagatcacc gatcggcctc ccgaatgaaa
     2761 gaaaatgccg accccagccg cgcgctggat agccgacctg tggaccagca tcttacacga
     2821 agacggctgc cttttcagca gcccccgccg ccgcgacgcg cgacgcagcc tacctcagcg
     2881 gcctcaggag agcgtcagca gcccccgatc catctccgtc aggacctcca cgccggtctc
     2941 ggtcaccgcc accgaatctt cgatgcgcac gccgcccacc ccttccaggt atatccccgg
     3001 ctcgatggtc accaccatgc ccggctccaa gacgtcctcc gagcgcgaac tgaggcccgg
     3061 ctcctcgtgc acttcgagcc cgaccccatg ccctaggccg tgtttgaaga agtcgccgag
     3121 gccttcgcct tcaatgacat cgcgcgccac cttgtccacg tggcggcagg cgatgcccgg
     3181 cccggcttct cgacgtgccg cctcctgcgc ctcgagcgtg acccggtggg cacgcgcctc
     3241 ttcgggcccc accttgcccg tagagaaggt ccttgtgatg tcgctcgcgt acccatcgac
     3301 cacggcgcca aggtccacca ccaacagccc gccctgcccg atgacggtgt cgcccggttc
     3361 cgcgtgaggc tttgcactat tgggccccca ggcaaggata taggggaacg ggatgccctc
     3421 agacccatgc ttccgcgccc acacctccag ctccaaggcc acatcccgct cggtgcggcc
     3481 caccacctgc atcagactga gtcgctccat cacctgcacg gcaaggagcg acgcttcgcg
     3541 gacggcccgc agttccccgg cggacttcac gcgacgcatc ttgcgaacgt gaccacgcac
     3601 cagcgatacg tccaacccct cgagggcggg cgcaagtgct tcccactcct ccactttcag
     3661 atggtccttc tccacgccga gacagccctc aaaaccttcc acaagttccg gggccagagg
     3721 agagaaggcg ccctccagct cgacgaaacg agcaaccggc gcctgttcgc gagcctgcac
     3781 cctatagcgg aagtccgaca cgagcaggca ctcgtcgggg cggacgagca cgactccagc
     3841 gctcccagtg aacccggtga gataccggat gttgaccggg tcgaagacga gaagacctga
     3901 cagaccatca agccccaacg aagaccgtaa cgtttccagc ctgtcgtcgt aaggcctgtc
     3961 gccggcgtca tcgaccactt ccaccactcc tctcgctttg cgactcatcc ggtccctctc
     4021 cacgcccgtc agaacacccc acggaagcga tcagcgcgcg cgccgcccgt cgatacccct
     4081 cgggtccctc cccggagacg gtcagcgaaa ccacgtctga tatcaccgaa acgcgccgcc
     4141 atgattcccg tgtggtcacg tccgacaagt gcacttcagc tatcggcgct tgcaccagct
     4201 ccaacgcatc gcggatcgcg tagctgtagt gggtccaagc tcccggattc acgagcatgg
     4261 ctccggcccg ggcgcgatac tggtggatca gccgaacgaa gcctccctcg tcatccgtct
     4321 ggtggcagat gcagcgccag ccgtgttcct cggcctcggc gctcaccagc gtctccaaat
     4381 cgcgcagggt gacggttcca tagtgggcgg ccggtctctc tccaagcagg ccgaggttca
     4441 caccgtgcag gagcacgagc agcccgccgc ccggttggtc gtcgggcctg tctcctttgg
     4501 aggtcgctgc atgcgtcata ccaggatcac cgccaatcga gccgcaagta gtccttcctc
     4561 gacgtggaca ccccacaccg ggtctcccac ggcccgcagg cacaccagac cgagccctgc
     4621 ggcgctcact ttcttgtcgc ggcccgcagc gtccatcaca tcgccttgcg tgacgcgcga
     4681 tcgtggtggg caggccgaag cgggcaagaa gcgcgcggac acggggaagc acctccttgt
     4741 tcaagccgca gctggactca ctgacggcca gcaccaccag caagccgagc ccgaccgcgg
     4801 ccccgtgcga caacgcgccg tatcccatcg catgctccag agcatgcgcc accgtgtgac
     4861 ccaggttcaa caccgctcgt cgcccatggt ccctctcgtc ttctacgacg atctccgcct
     4921 tcgaccgcac gcaccgctcc acgatcccgc tcacccgtga cgggtcgcga gccaagatgt
     4981 ccgccgcgcc cgactccaac tcccgcagga aaggctcgcc cgagagaagc gcgtacttga
     5041 ccacctcgcc cagacccgag gcgaagtcca gctcgggcaa agaccgcagt acactctgat
     5101 cggagaccac caactccggc tgatagaacg cacccaccag gttcttgccg cccgcgaggt
     5161 tgacccccac cttaccgccc acgctggaat cgacctgagc cagaagggtg gtggggatct
     5221 gccagaagcg gacgccgcga aggtaggtgg ctgcgacgaa tccggccaag tcgcccacaa
     5281 ccccgcctcc gactgcgacc acgatgtcgt ccctccgggc gccttgatcg accagccact
     5341 cccagcaccc gaccgcgacc tcaatgctct tcgacgcttc tccaggcgtc aacaggtaga
     5401 ccgccggagc gcagtcgccg gagctgagcg cgcgcaggac ttccgccccc acgtggaccc
     5461 acaccgcctg gtcggagacg acgaagagtc gggagcccgc tcggctcgct ccagccacgc
     5521 gcgacggcaa cccatccagt gagtcccatc caccgactat cgtcgacaaa ggaggagatg
     5581 ccccgacttc caccttccag agcgatccgc tggtcggccg agaggacacc ccactcactt
     5641 cggcctcgcg ttgacgcacg gctgcaacaa ccagctgcgc cgcctcttca gtgtcaaggc
     5701 ccgacacatc aagcaccaca tccgcggtgc ggagatacga cgctcgacgc gcgtcggcaa
     5761 gagcggcaaa ctcttctttc gattgcgcga gcgggcgtcc tgtgtggcgc acccgttccc
     5821 aaaggatctc cgtctgcgcc gacagcagga gcacgaagcc ccccgccaaa cgctccgcgg
     5881 actcggccca ggtgactgtg cctcctccga gggcgaccac gatccctccc gccacctccg
     5941 ccgctgagtc gagtacctcc gcgagcgccc gctcctcgac tctcctgaat ccttcctctc
     6001 cccgctccgc gaagatgctc tcgatcgacg c
//
LOCUS       DASRMJ010000125         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_229394, whole genome shotgun sequence.
ACCESSION   DASRMJ010000125 DASRMJ010000000
VERSION     DASRMJ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229394"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..3171)
                     /locus_tag="VFD74_03600"
     CDS             complement(1..3171)
                     /locus_tag="VFD74_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZK48668.1"
                     /translation="MSWLTRISLRNRSVVALAVIAVVLVGGFAITSLRQELIPNIEFP
                     ALTVFTMEPGASPADVERGVTTPLEAGIKGLPGLQDLTSYSNEGMSIIVAMFEYGADM
                     EANQAAVQQVIDRAQFALPSGVQPRVQAISFGDMPVIQLAVSSPLPPEQLAGLVQTRV
                     LAKLQQVPGVADVSLSGVSSSQLQIEIQPMAMATLGVSPQAIAAALQAANVSMPAGTL
                     TTDGASVPVRVTSRSTSLEALAALPVKTSGAASAAAGITGGAVGGAPGGAAQPGATAG
                     AASAGSPTSPAAPVTLGQVAKLSIVPSAATSITRTNGEPSVGIAVTKSADGNVVDISN
                     AVADLLPQIEADLGEGAVVTTVVDQAPYITQSIRSMWEEGILGAFFAILVILLFLRSW
                     RSTIVAGISIPLSVIVALIILWGRNNSLNMLTLSGLTIAIGRVIDDAIVVLENAYRHL
                     QEGDDPRTAAYTATREVAAAITASTLTTVAVFLPLGFVGGISSEFFRPFALTVTFALL
                     ASLVVAFTVVPVAVTLLLSKKQVGHRESNQTTLLQRLYLPALRFAISHKLITVVAAFA
                     VFGGSLALTPLLQTNLMDQSGETGLTITQQLPAGTETAVTSDAAAKLEAMLAETPGVV
                     SYQVTIGSTGGLFGPGGGVNASGARAAFTVNTDGKLDKTAMIDDLRARVDATGGLGDV
                     SFSAGESMGGDTSVIEVRVYANDPTVLEEANSAVVTRLGSVSGLASVASNLSERMPQI
                     AVKVDPAKAAAAGLDATSIGGYVGLVLNGMPLGTVPTDQGPLAAFVRFPAMAGMTSET
                     FGNLPLASATGIVPLSSVADVTLVQEPVQVTHTDGERTVTVSVTSLGNNIGDTSTNIR
                     TALEGLQLPDGSRWELSGATAQMDDMFRSLGLAMLVAIIVVYLIMVGTFRSLLNPMIL
                     LVSIPFAAVGAIVLMLLTGTSLGMPSLIGLLMLIGIVVTNAIVLLDLVEQFRDQGMDA
                     HTAVIEGGRRRLRPILMTAVATILALSPMALGLGEGSFLSRPLAVVVIGGLISSTAMT
                     LIIVPVVYLVFDRLRRRPEKIEFAAD"
     gene            complement(3168..3668)
                     /locus_tag="VFD74_03605"
     CDS             complement(3168..3668)
                     /locus_tag="VFD74_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK48669.1"
                     /translation="MDQADRPAGQAQVIEDFGLAFEDHGMPRMAGRILGRLLMCDPPR
                     QSMDQLVEALSASKGSVSSMTRLLIERGVIERLSLPGDRHDYFRIRTGSMAGMFREHA
                     HRSIIPLLLLERAMEAADDRSVEGLARLRDSRDFLAFMQRELPALLQRWDTLHTQELS
                     TQKEDE"
     gene            4006..>4255
                     /locus_tag="VFD74_03610"
     CDS             4006..>4255
                     /locus_tag="VFD74_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZK48670.1"
                     /translation="MSSKLYVGNLAYDATDAALEELFSAYGTVTSVALISDRDTGRPK
                     GFGFVEMSKEEEALAAKAALDGTEFGGRSIKVDVAKERP"
BASE COUNT          725 a         1317 c         1498 g          715 t
ORIGIN      
        1 tcagtcggct gcgaactcga tcttctccgg ccggcggcgc agcctgtcga acaccaggta
       61 gaccaccggc acgatgatga gtgtcatggc cgtggacgag atcaggcctc cgatgaccac
      121 caccgccagc ggtcgcgaca gaaacgagcc ctcgcctagg ccgagggcca tgggggagag
      181 ggccaggatg gtggccacgg ccgtcatgag gatggggcgc agtcggcgcc gaccgccctc
      241 gatcacagcg gtgtgggcgt ccatcccttg gtcacggaac tgctccacca gatcgagcag
      301 aacgatggcg ttggtcacca cgataccgat caacatgagc aggcctatga ggctgggcat
      361 gcccaggctc gtaccggtga gcagcatgag gacgatggcg cccacagctg cgaacgggat
      421 gctcaccaag aggatcatcg ggttgaggag gctgcggaag gtgcccacca tgatcaagta
      481 gaccacgatg atcgccacca gcatcgccaa cccgaggctg cggaacatgt cgtccatctg
      541 agcggtggct cccgacagct cccagcgtga cccatcgggc agctggagcc cttcgagggc
      601 ggtgcggatg ttggtggagg tgtctcctat gttgttgccc agggaggtca ccgacacggt
      661 gaccgtgcgc tcgccgtcgg tgtgcgtcac ctggacgggc tcctgaacca gggtcacgtc
      721 cgccaccgag gacaggggca cgatgcccgt cgcgctcgcg agcggcaggt tgccgaacgt
      781 ctccgaagtc atccctgcca tggcggggaa ccgtacgaag gcggcgaggg ggccttggtc
      841 ggtgggcacg gtgcccagcg gcatgccgtt caggacgagt ccgacgtaac ccccgatgga
      901 ggtggcgtcg agaccggccg ccgcggcctt ggccgggtcc accttgactg cgatctgcgg
      961 catgcgctcc gagaggttgg aggcgacgga ggcgaggccg ctcacgctac cgagtcgcgt
     1021 gacgacggcg gagttggctt cttcgagcac ggtcgggtcg ttcgcgtaga cgcgcacttc
     1081 gattaccgag gtgtcgccac ccatggactc cccagcgctg aacgagacat cgccgagccc
     1141 gccggtcgca tcgacgcggg cgcggagatc gtcgatcatc gcggtcttgt cgagcttgcc
     1201 gtcggtgttc acggtgaagg ccgcgcgggc gcccgaggcg ttgacgcctc cgccgggacc
     1261 gaagagtccg ccggtgctgc cgatggtcac ctgataggac acgacgccgg gggtctcggc
     1321 cagcattgcc tcgagcttgg ccgctgcgtc cgaggtgacg gccgtctccg tgcccgccgg
     1381 gagctgctgg gtgatggtca gccctgtctc accggactgg tccatcaagt tggtctggag
     1441 caggggagta agggccaggg agccgccgaa gacggcgaac gcggccacca ctgtgatcag
     1501 cttgtgcgaa atggcgaagc ggagcgccgg aaggtagagg cgttggagaa gggtggtctg
     1561 gttgctctcg cggtgcccca cctgcttctt ggagagcagg agcgtgaccg caaccggcac
     1621 cacagtgaag gcgacgacca ggctggccag caacgcgaag gtcaccgtca gcgcgaaggg
     1681 gcggaagaac tcggaggaga taccgcccac gaagcccaaa ggcaggaaca ccgccaccgt
     1741 cgtgagggtg ctggccgtga tggccgcggc gacctcgcgg gtggccgtgt aggccgcggt
     1801 gcgggggtcg tcgccctcct ggaggtggcg ataggcgttc tcgagcacca cgatggcgtc
     1861 gtctatcacg cgtccgatgg cgatggtgag gccggagagc gtgagcatgt tcagagagtt
     1921 gttgcgtccc cacagaatga tcagcgccac gatgacgctg agggggatcg atatcccggc
     1981 aacgatggtg gagcgccaac tgcggaggaa gagcaagatc acaagtattg cgaagaatgc
     2041 tcctagtatc ccttcctccc acatcgaccg gatgctctgc gtgatgtagg gggcttggtc
     2101 gacgaccgtc gtgaccacgg caccctcgcc gaggtcggct tcgatctggg gcagaaggtc
     2161 ggcgaccgcg ttggagatgt ccacgacgtt gccgtctgcc gacttggtga cagcgatgcc
     2221 cacgctgggt tcgccgttgg tgcgggttat ggaggtcgcg gccgagggga cgatgctgag
     2281 cttggccacc tggccgagcg tgaccggcgc agcgggcgag gtcggggagc cggcggatgc
     2341 cgctccggcc gtcgcgcccg gctgcgcggc tccgcctgga gcgccgccca ctgcgccgcc
     2401 ggttatcccg gcggcggcgg aagccgcgcc ggaggtcttg accggaaggg cggccagggc
     2461 ctcaagactg gttgatcggc tggtgacgcg caccgggacg ctggcaccgt cggtggtcag
     2521 ggtgcctgcc ggcatcgaga cgttggccgc ctgcagcgcg gccgcgatcg cctggggcga
     2581 gacgccgaga gtggccatcg ccatgggctg gatctctatc tgcagctggg agctcgacac
     2641 ccccgacaag gatacgtccg cgacgcccgg cacctgctgg agcttggcga gcacgcgtgt
     2701 ctggacgagt ccggccagtt gctccggcgg cagcggcgaa ctcaccgcca gttggatcac
     2761 aggcatatca ccgaagctga tggcctggac ccgcggctgg accccgcttg gaagcgcgaa
     2821 ctgggcgcgg tcgattacct gctgcacggc cgcttggttg gcctccatat ccgctccgta
     2881 ctcgaacatg gccacgatga tgctcatgcc ctcgttcgag taagacgtga ggtcctgaag
     2941 accggggagc cccttgatgc ccgcttcgag cggggtggtg acgcctcgct ccacgtcggc
     3001 cggagaggcg cccggttcca tggtgaacac cgtcaaggcc gggaactcga tgttcggtat
     3061 cagttcctgg cgcagcgagg ttatggcgaa gccgccgacc agcaccacgg cgatgacggc
     3121 gagcgccacc accgagcggt ttcgaaggga gattcgggtg agccagctca ttcgtcctct
     3181 ttctgagtcg agagttcttg ggtgtggagc gtgtcccaac gctgcagcag cgccggcagt
     3241 tcccgctgca tgaaggccag gaagtcgcgg gagtcacgta gtcgggccaa tccctccacc
     3301 gagcggtcgt cggccgcctc catcgctcgt tcgagcagca ggagagggat gatcgaacgg
     3361 tgggcgtgtt ctcggaacat gcctgccatg gacccggtgc ggatgcggaa gtagtcgtgg
     3421 cggtcccccg gcagggacag tcgctcgatc acgccacgtt cgatgagcag ccgagtcatc
     3481 gaactcacgg agcccttgct cgcgcttagg gcctccacga gttgatccat cgactggcgc
     3541 ggtggatcgc acatcagcag gcgcccgagg atgcgtccgg ccatgcgcgg catgccgtgg
     3601 tcttcgaagg ccagtccgaa gtcttcgatc acctgcgctt gcccggcggg gcgatccgct
     3661 tggtccatcc aggtgccttt cgtgaagctt caagtcgtgt gttcatcgtg tggccgggag
     3721 ccctgcgccc ccatgcggta cgatgcgggt ctccgcgagt ccgtccagca ggggcgtcgt
     3781 cggcgacgcg gcatactacc attcagtccg ttcagtcaca actgaacacc agtcgtcgca
     3841 aggcgctctt gagaccggtt ttggaccgcg cgacgatgtg atacgttgtg ttgtgcatcc
     3901 gataggaccc cactgcaagc gctgcgccga gtgtctcggg tttccgcctc ctttgcagga
     3961 tattcctctc gtttgcggca ggatacatct cgcactggag gatcgatgag ttcgaagctg
     4021 tacgtgggca acctggccta cgacgcgacc gatgccgctc tggaggagct cttctccgca
     4081 tacggcacgg tcacctcggt cgcgctgatc agcgaccgcg acacgggccg ccccaagggg
     4141 ttcggcttcg tggagatgtc caaggaagag gaagcgctgg ccgccaaagc cgctctcgac
     4201 ggcactgagt tcggcggtcg gtccatcaag gtggacgtgg cgaaagagcg tcccc
//
LOCUS       DASRMJ010000126         8343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_229397, whole genome shotgun sequence.
ACCESSION   DASRMJ010000126 DASRMJ010000000
VERSION     DASRMJ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229397"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1659
                     /gene="hemA"
                     /locus_tag="VFD74_03615"
     CDS             229..1659
                     /gene="hemA"
                     /locus_tag="VFD74_03615"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZK48671.1"
                     /translation="MHIAAIGVNHKTACLEVRERVALTDAAAQGLLARLLARAELGEA
                     VAVSTCNRTEIYVTGESLPLREAVLEELCVATGVCREDVVESVYYHEDDRAVDHLFAV
                     AASLDSMVLGEAQILHQLKSAYQTAEEAKTTGAVLNKLFRRSFEVGKRVRSETRIGEN
                     SLSIASVSVDLARNVFGDLSSRSVLVVGAGEMAELVLTHLKGHGVSHVRVANRTFERA
                     AELAERFDGDAVHYERLLEHLMAVDIVITSTAATQPIIGRKDMERVMKGRKNRPIFLV
                     DIAVPRDVEATVNGVYNAYLYDIDDLQEVVASNLGERKQEAATAREIVSEEVEAFRAW
                     LRSLQVVPTMVDLRRWATGVKDEELERYLARMPDLTEDQREKVAALAHSLVNKLLHPP
                     TVRVKEAASEGSGYRYSESLRELFGLDEQPRAADPPPPADAPPVAVASAGTAAAGTER
                     AASGTAGAPPGAAGAPAEHIEERSTS"
     gene            1656..2588
                     /gene="hemC"
                     /locus_tag="VFD74_03620"
     CDS             1656..2588
                     /gene="hemC"
                     /locus_tag="VFD74_03620"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017219407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZK48672.1"
                     /translation="MNTQRVVIASRRSRLAMTQSRYVQALLEGLEPGLEVAILEIVTS
                     GDKILDSPLSQIGDRGLFVKEIEEALLSGDADLAVHSAKDVPTALPEGLDLLAFPERE
                     DPSDVFVGSLESSAGLTSGRRIGTSSLRRRSQLLARFPGLEIVDIRGNVETRIRKIQE
                     DGLDGTILAAAGLKRLGRFDEAAFSFSDEDMLSAVGQGSLAIEARRGDQRVAALLARL
                     DHAPTRAEVLAERALMRALEGGCQVPIGARARLTGDVLHLVAYVGSIDGSRSVRMGRA
                     GSAEAPAELGEELAAEMLVAGADEILAQVRGATG"
     gene            2708..3460
                     /locus_tag="VFD74_03625"
     CDS             2708..3460
                     /locus_tag="VFD74_03625"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZK48673.1"
                     /translation="MTRPRAQAAGQIEALRALGAEVIVFPTIRVEPVLASQEIELMTS
                     AVGSYDLVVFTSVNAVDCFFERLREVGKDACALGRASVVAVGPKTAAACSAHGAAPDT
                     VPDTFVAEGVLQALAGRDLSSTRILMPRAREARETLPESFAAAGAVVDVVALYDTLPE
                     RYTAEEIELALGADYVTFTSGSAARSFAALLRFGGFGAELARVAATSIGPVTSRVVRE
                     EGMRVLVEAESYTIDGLVEAVVAHTVYSPGSG"
     gene            3563..4567
                     /gene="hemB"
                     /locus_tag="VFD74_03630"
     CDS             3563..4567
                     /gene="hemB"
                     /locus_tag="VFD74_03630"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZK48674.1"
                     /translation="MYFPTYRPRRMRQTDSLRSMIRETHLHPHMFIYPLFATFGEDVR
                     RPISSMPGVFQFSINNLVEEAKEVAALGIRSILLFGLPEHKDEVGSSAYDSEGITQMA
                     IRAIKEHVPQLTVVADVCLCEYTSHGHCGVVRADGCVDNDLSLELLAKTAVSYAEAGV
                     DVVAPSDMMDGRIGAIRSALDQDGHSDVPIFSYSTKYASSFYGPFREAADCALQFGDR
                     KSYQMDPANSDEGVREAFLDIEEGADMIMVKPALPYLDLIYRLKTETRFPVAAYNVSG
                     EYAMLKAAAERGWLDEKAVVMETLLSIRRAGADVIITYHAKDAARWLGERMPIVPRKP
                     "
     gene            4564..5790
                     /gene="ahbD"
                     /locus_tag="VFD74_03635"
     CDS             4564..5790
                     /gene="ahbD"
                     /locus_tag="VFD74_03635"
                     /EC_number="1.3.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HZK48675.1"
                     /translation="MSAHPHGGPHGDPGVPRKAGHPPDVAASTPAGAAAEPGRVAYSP
                     KLIAWEITRSCNLSCVHCRASAEKGPYPGELDLEQAKAVIDDIVSFSNPVLILTGGDP
                     LMREDIWEVVEYAIGSGLRTVMSPNGTLVTPEAARKMKELGVPRISISIDFPTAELHD
                     KFRGVPGAFDGAVRGVKTAIEAGVQVQINSTITRLNVEYLDDLLALAEDLGAVALHPF
                     LLVPTGRGKELGPEELPPEDYERTLNWVYDRQKTSKIFFKPTDAPHYHRIMRQRAKAD
                     GTDLSELKMHPHSHAAMAGGVGKAGHPGDLDSHSRGCLAGTGFCFISHVGHVQPCGYF
                     DVVAGDVKQQPFSEIWRDAPLFQDLRDVDKLKGKCGICEYRRVCGGCRARAYEVSGDY
                     LAEEPYCVYEPVRRGC"
     gene            5895..6968
                     /locus_tag="VFD74_03640"
     CDS             5895..6968
                     /locus_tag="VFD74_03640"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZK48676.1"
                     /translation="MSTASRTYVLDTNALLNDPDVIYSFSGAEVVIPAVVIEELDKIK
                     RRRADRRIRFHGRKATRLLFELSQHGRLVDGIRLVNGSLVRIDEMHDMGDQPPELDLK
                     RPDDQILALTRSLNGGPGVHATLVSNDLNLLLRAELLGLGTYRFEGKLEHMVDSRPSP
                     TEWFREHGFTLALGVLAALLLFSTAYLYYSRPAPNLLADLNVADDAGVLRDLGVSVQV
                     LEGYYRDRLAGDSTDVSAMVNLGNLLFDQERYLEAVEFYRSALAIDPEKPSVRTDLGI
                     ALLNLGHYKEAVQAFEQATRDAPEHALAHYNLGIALAQGGDQARALEELETAINLAAD
                     TGAVPLDTARSLIADLRAKLGGG"
     gene            7089..>8343
                     /locus_tag="VFD74_03645"
     CDS             7089..>8343
                     /locus_tag="VFD74_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZK48677.1"
                     /translation="MRKRIAWIALSGLVLLAFVTVLGFRETSKPSFCSSCHMIEPYKA
                     SWDTSTHAAVGVGCVDCHFEPGAVGYTKGKLYSFIKLTQFAAGSTEKKPEAGKLVLGA
                     ACIQCHDYVRNPDDPRYPTGIIVEGITFPHQFHVDEANLLCSDCHSGIVHGSALVGAE
                     KPQAAADPSFCNTCHTGDFAPILFTAIESAGREHPGAPKIDVNVWRNIHWRVAGAPAV
                     VDGVQYDKIEKDTCLACHQEPTVAKACKSCHAARVPEFSASPQAARASMIPVVLFAFL
                     FALFMVSVFLRRHEKERFFSSVVLRIVAVAVVLSDAFVVYLIVNDVLREQTGHHEVGP
                     TTVWVSYLLLSVALVAFLLFEAGLLPSPLHKVGLPREDEDEFLVPKPIRRMKARTSGS
                     PSSSEGSPSEGPSGGDASSADRAAAH"
BASE COUNT         1376 a         2831 c         2707 g         1429 t
ORIGIN      
        1 gtcagttggg tcttctacgg cggctacttg gtcctgcggg agacggccag ctggagagga
       61 aggaatgccg ctctgatcgc gatcgcaggc atgttcctca tcgtgttcac gttcttgggc
      121 ataaacctgt tcgtgccggg gcgccatgat ttcaactgag ttctcgacgt cggtccgact
      181 cgcacactca gcagtcgtcg acgcgtcgac ctaagaggct acggccgcat gcatatcgcc
      241 gccatcggag tgaatcacaa gaccgcctgt ctcgaggtgc gcgagcgcgt ggctctgacc
      301 gacgccgccg cgcagggact tctcgcacgc cttctcgcgc gcgccgaact gggcgaagcc
      361 gttgccgtga gcacctgcaa ccgcacggag atctacgtca ccggggagtc tctccccctc
      421 agagaagccg tgctggagga gttgtgtgtc gccaccggcg tctgtcgcga agacgtcgtc
      481 gagagcgtct actaccacga agacgatcga gcggtcgacc acctgttcgc cgtcgccgcc
      541 tccctcgact ccatggtcct cggtgaggcg cagatactgc accagctgaa gtcggcgtac
      601 cagacagccg aagaggcgaa gacgaccggg gctgtgctca acaagctgtt ccgtcgctcc
      661 ttcgaggtgg gcaagcgggt gcgcagcgag acccgcatcg gcgagaactc gctctcgatc
      721 gcatcggtct ccgtcgacct ggcccgcaac gtcttcggcg atctgtcgtc gcgctcggtg
      781 ttggtggtcg gggcggggga gatggccgag ctggtgctca cccacttgaa gggtcacggc
      841 gtcagccacg tgcgggtggc caaccgcacc ttcgagaggg cagccgaact cgccgagcgg
      901 ttcgacggcg acgccgtaca ctacgaacgg ctgctggagc acctgatggc ggtggacatc
      961 gtcatcacct cgacggcggc cacgcagccg atcatcggcc gcaaggacat ggagcgggtg
     1021 atgaagggcc gcaagaaccg ccccatcttc ttggtggata tcgccgttcc gcgcgacgtc
     1081 gaggcgacgg tcaacggcgt ctacaatgcc tacctctatg acatcgatga cctccaagag
     1141 gtcgtggcct ccaatctcgg cgagcgcaaa caggaagccg ccaccgcccg cgagatcgtc
     1201 tccgaggagg tcgaagcatt ccgcgcgtgg ctccgctctc tgcaggtcgt gcccaccatg
     1261 gtcgaccttc gccgctgggc caccggcgtc aaggacgagg agttggagag gtacctggct
     1321 cggatgcccg acctcaccga ggatcagcgc gagaaggtcg ccgccctggc ccactctctc
     1381 gtgaacaaac tgctccatcc tcccaccgtg cgcgtcaagg aagccgccag cgaggggagc
     1441 gggtaccgct actccgagtc gctgcgagag ttgttcggtc tcgacgaaca gccgcgggcc
     1501 gcggacccac caccgcccgc cgacgcgccg cctgtcgcgg tcgcgtccgc aggcaccgcg
     1561 gctgccggca ccgagcgggc ggcctcaggc acagcgggag cgcccccggg cgccgcggga
     1621 gcgcccgccg aacacatcga agagaggagc acgtcataaa cacgcagcgt gtggtcatcg
     1681 catcgcgtcg gagccggctg gccatgacgc agagccgcta cgtacaggcg cttctggaag
     1741 gactcgagcc cgggctcgag gtggctatcc tggagatcgt caccagcggg gacaagatcc
     1801 tcgactctcc tctctcccag atcggggacc gaggcctttt cgtgaaggag atcgaggagg
     1861 cgctcctctc gggtgacgca gacctggctg tgcacagcgc caaggacgtg cccactgcgc
     1921 tgcccgaagg tctcgatctc ctcgcgttcc cggagcgtga ggatccatcc gatgtgttcg
     1981 tgggttctct tgagtcgtcc gcgggcctga cgtcgggacg gcgcatcggc acgagcagtc
     2041 tgcgtcgccg ttcccaactc ctcgcccgct tcccgggtct cgagatagtg gacatccgcg
     2101 ggaacgtcga gacccgcatc cgcaagatac aagaggatgg gctcgacggc acgatccttg
     2161 cggccgccgg cctgaagcgc ctgggtcgtt tcgacgaggc cgcgttctca ttctccgacg
     2221 aggacatgct gagtgcggtg gggcagggct ccctcgccat cgaggctcgg cgcggcgacc
     2281 agcgcgtggc cgcgctgctc gcccgcttag atcacgcccc cacgagggcc gaggtgctgg
     2341 ccgaacgggc attgatgcgc gctctggagg gcgggtgcca ggtccccatc ggcgcccggg
     2401 cgcgcctgac aggagacgtc ttgcacctcg tcgcctacgt cggctcgatc gacggctcgc
     2461 gcagcgtacg catggggcgc gcgggatcgg ccgaggctcc tgcggagctc ggcgaggagc
     2521 tggcggccga gatgctcgtt gccggcgccg acgagatcct ggcccaggtg cgaggggcga
     2581 ccgggtgagt cacgaaagga cggcgccgcc cgcgggcgag ggagcgtctt cgggcgatcc
     2641 cggtcccgcg cggcatcgcg ccggtcctgt gccaggggaa ctccctctgg cgggtcggcg
     2701 tgtcgtcgtg acgcggccgc gggcgcaggc cgccggccag atcgaggctc tgcgcgcact
     2761 gggagcggag gtcatcgtct tcccgacgat acgcgtcgaa cccgtgttgg cgtcacagga
     2821 gatcgagctc atgacctcgg ccgtgggatc gtacgaccta gtcgtgttca ccagcgtcaa
     2881 cgcggtcgac tgcttcttcg aacgactccg cgaggtcggc aaggatgcct gcgccttggg
     2941 gcgagcctcg gttgtcgctg tcggccccaa gaccgccgcg gcgtgctcgg cacacggtgc
     3001 ggcccccgac acggtcccgg acacgttcgt ggccgagggg gtgctgcagg ctctcgccgg
     3061 tcgcgacctg tcaagcaccc gcatcctgat gccccgggcg cgcgaggcga gggagaccct
     3121 gccggagtcg tttgccgcgg cgggagccgt ggtggacgtg gtcgccctct acgacaccct
     3181 ccccgaacgc tataccgccg aggagatcga gctcgctctc ggcgccgatt acgtcacctt
     3241 cacctccggc tcggcggcgc gcagtttcgc ggcgttgttg cgtttcggag gtttcggcgc
     3301 cgaactcgcc cgcgtcgcgg cgacttccat cggtccggtg acgtctcggg tcgtgcgcga
     3361 agaaggcatg cgtgttctcg tggaagccga gagctacacg atcgacggcc tcgtggaggc
     3421 cgttgtcgcc catacggtat actcgcccgg ttcaggttag cccgcccgcg ctcccgaagt
     3481 cgcgtacgtc gccgccggat cagcccggcg acaggggagc gttcggcccc atctttcggt
     3541 cagtcggagg tcttggcaga caatgtactt ccccacctat cgtccgcggc gcatgcggca
     3601 gaccgacagt ctccgctcca tgatccgcga gacccaccta cacccgcaca tgttcatcta
     3661 ccctctgttc gccaccttcg gagaagatgt ccgtcggccg atatcgtcca tgccgggcgt
     3721 gttccaattc tccatcaaca acctcgtgga ggaagcgaaa gaggtcgccg cgctgggcat
     3781 ccggagcatc ctgctcttcg ggctgcccga gcacaaggac gaggtgggtt cctccgcata
     3841 cgattccgag gggatcaccc agatggcgat ccgcgccatc aaggagcacg taccgcaact
     3901 caccgtggtg gccgacgtgt gcctctgcga gtacacgagc cacgggcact gcggcgtcgt
     3961 gcgcgccgac ggctgcgtcg acaacgactt gtcgttggag ttgctggcca agacggccgt
     4021 cagctacgcg gaggccggcg tggacgtcgt ggcgccgtcg gacatgatgg acgggcgcat
     4081 aggcgccatc cgctcggcgc tcgaccaaga cggccacagc gatgtgccga tcttctccta
     4141 ctccaccaaa tacgcgtcga gcttctatgg acccttccgc gaggcggccg actgcgctct
     4201 ccagttcgga gaccgcaagt cgtaccagat ggacccggcc aactccgacg aaggcgtgcg
     4261 cgaagccttc ctggatatcg aggagggcgc cgacatgatc atggtcaagc cggcgcttcc
     4321 ttacctggac ttgatctacc ggctcaagac cgagacgcgc ttccccgtgg ccgcctacaa
     4381 cgtgagcggc gagtacgcga tgctcaaggc cgccgccgag cgcgggtggc tggacgagaa
     4441 ggccgtcgtc atggagaccc tgctctccat ccgtcgcgcc ggcgccgacg tgatcatcac
     4501 ctaccacgcc aaggacgcgg cacgctggct cggcgagcgt atgcccatcg tcccgcgaaa
     4561 gccatgagcg cccacccgca cggcggcccg cacggcgatc cgggagtccc tcggaaggcc
     4621 ggccatccgc cggacgtcgc ggcctcgacg cccgcaggcg cggccgcgga gcccggccgg
     4681 gtcgcctact cccccaaact tatcgcctgg gagatcaccc gttcgtgcaa cctctcctgc
     4741 gtgcactgtc gtgcatcggc ggagaagggt ccctacccgg gcgaactcga cctggagcag
     4801 gccaaggccg tcatcgacga catagtatct ttcagcaacc cggtcctcat cttgacgggt
     4861 ggcgaccccc tcatgcgcga ggacatctgg gaggttgttg agtacgcgat cggctccggc
     4921 ctgcgcacgg tgatgtcgcc caacggcaca ctggtcacgc ccgaggcggc ccgcaagatg
     4981 aaggagctcg gcgtcccgcg catctccatc agcatcgact tccccacggc cgagctgcac
     5041 gacaagttcc gcggcgttcc cggggccttc gacggtgccg tgcgcggcgt caagaccgcg
     5101 atcgaggccg gggtccaggt gcagatcaac tccaccatca cccgcctgaa cgtcgagtac
     5161 ctcgacgatc tgctcgccct ggccgaagac ctaggagcgg tcgcgctgca tcccttcctg
     5221 ctggtgccca ccggccgcgg caaggagctc ggacctgaag agctgcctcc cgaggactac
     5281 gagcgcaccc tgaactgggt ctacgatcgc cagaagactt caaagatctt cttcaagccc
     5341 accgacgcac cgcactatca ccgcatcatg cggcagcggg cgaaggcaga cggcaccgat
     5401 ctctcggaac tcaagatgca tccgcactcc cacgccgcca tggcgggcgg ggtagggaag
     5461 gccggccatc ccggcgacct ggattcccac tcgcgaggct gtcttgccgg cacggggttc
     5521 tgcttcatca gtcacgtggg tcacgtacaa ccgtgcggct acttcgacgt ggtcgccggc
     5581 gacgtgaagc agcagccgtt ctcggagatc tggcgtgacg caccgctctt ccaggacctg
     5641 cgcgacgtcg acaagctcaa gggcaagtgc ggcatctgcg aatatcggcg cgtctgcgga
     5701 ggttgtcgag ctcgcgccta cgaggtgagc ggcgactatc tggccgaaga gccctactgc
     5761 gtgtacgagc ccgtgcgaag gggttgctga ggccgagtcc ccccgggctt cgtccgtggc
     5821 tcgggccgcg gacgggccgc tcgggccgcg gacgggccgc tcgggccgcg gaactcctgt
     5881 gctaccatcg gactatgtcg accgcttcac gaacctacgt tcttgacacc aatgcgctgc
     5941 tgaacgaccc ggatgtgatc tactccttct cgggggccga ggtggttatc ccggcggtcg
     6001 tcatcgagga gttggacaag atcaagagga ggcgggccga tcggcggatc cgcttccacg
     6061 gccgaaaggc cacccggttg ctcttcgaac tctctcagca cggcaggttg gtcgatggca
     6121 tccgcttggt caacggctcc ctcgtgcgta tcgacgagat gcacgacatg ggcgatcaac
     6181 cgcccgaact cgacctgaaa cggccggacg atcagatcct cgcgttgacc cgctcgctga
     6241 acggcggccc cggtgtgcac gccacgttgg tctcgaacga cctcaacctg ctgctgcggg
     6301 ccgaactcct gggcttgggc acctaccgct tcgagggcaa gcttgagcac atggtcgaca
     6361 gccggcccag ccccaccgag tggttcagag agcacgggtt caccctggcg ctcggggtgc
     6421 tggcagcgtt gctgctcttc tccacggcct acttgtacta ctcgcgcccg gcgccgaacc
     6481 tcctcgccga tctgaacgtg gccgacgacg cgggagtgct gcgcgacctc ggtgtgagcg
     6541 tccaggtgct cgaggggtac taccgcgaca ggctggccgg ggactccacc gatgtctcgg
     6601 cgatggtcaa tctggggaac cttctcttcg accaggagag gtatctcgag gcggtggagt
     6661 tctatcgaag tgctctcgcc atcgatccgg agaagcccag cgtccgcacc gacctcggta
     6721 tcgcgcttct caatctcggg cactacaaag aggcggtcca ggcattcgag caagcgaccc
     6781 gcgacgctcc cgaacacgcg ctggctcact acaatctggg catcgctctt gcgcagggcg
     6841 gcgaccaggc ccgggcgctc gaagaactgg agaccgcgat caatctcgcg gccgacaccg
     6901 gcgcggtccc cttggatacg gcccggtccc tgatcgcgga tctgcgagcg aagctgggcg
     6961 ggggctgatt cggagggatc attctcgctg cggatcattg gcatcccggt tcctcgggta
     7021 ttccctcgac gaaccgtgtc cgacagtggt agcatacggg cgttccttgt cagctccagg
     7081 gggaagtctt gcgcaagcgc atcgcttgga tagcattatc cggtcttgtc ctgctcgctt
     7141 tcgtgacggt gctcggattc cgcgagacct cgaaaccctc cttctgttcc agttgccaca
     7201 tgatcgagcc atacaaggcc tcgtgggaca cctccaccca tgcggccgta ggcgtcgggt
     7261 gcgtcgattg ccacttcgaa cccggggccg ttggctacac caagggcaag ctctactcgt
     7321 tcatcaagct cacccagttc gccgccgggt ccaccgagaa gaagccggaa gccgggaagt
     7381 tggtcctggg tgccgcctgt atccagtgcc acgactacgt gcgcaatccc gacgaccctc
     7441 gctatcccac aggcatcatt gtggagggga tcaccttccc gcaccagttc cacgtggacg
     7501 aagcgaacct gctctgcagt gactgtcaca gcggcatcgt ccacggctcc gccctcgtcg
     7561 gagcagagaa gccccaggct gccgccgacc ccagcttctg caacacctgt cacaccggcg
     7621 acttcgcgcc catcctcttc acggccatcg agtccgcggg gcgggagcat ccgggtgccc
     7681 ccaagatcga cgtcaacgtg tggcgcaaca tccactggcg cgtcgcgggc gcccccgccg
     7741 tcgtcgacgg tgtccagtac gacaagatcg agaaggacac ctgcctggcc tgccaccagg
     7801 agcccaccgt ggccaaggcg tgcaagagtt gtcacgccgc ccgtgtgccg gagttctccg
     7861 cgtcgcctca ggcggcccgg gccagcatga tcccggtcgt gctcttcgcc ttcctgtttg
     7921 ccctcttcat ggtgtcggtc ttcctgcgaa ggcacgagaa ggaacgcttc ttctcgtcgg
     7981 tcgtgctgcg aatcgtcgcc gtcgcggtcg tcttgtccga cgctttcgtt gtctacttga
     8041 tcgtgaacga cgtgctgcgt gagcagaccg gccaccacga ggttgggccg accaccgtgt
     8101 gggtcagcta tctcctgctc agcgtggccc tggttgcgtt cctgctgttc gaggccgggt
     8161 tactgcccag ccccctgcac aaggtcggtc ttccacgcga ggatgaggat gagttcctcg
     8221 tgccgaagcc catccgcagg atgaaggcga ggacgtcggg gagcccgtcc tcgagcgagg
     8281 gttccccgag cgagggcccg tccggtggtg atgcctccag tgcggatcgg gccgccgccc
     8341 acc
//
LOCUS       DASRMJ010000127         4908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_371422, whole genome shotgun sequence.
ACCESSION   DASRMJ010000127 DASRMJ010000000
VERSION     DASRMJ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371422"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VFD74_03650"
     CDS             <1..1587
                     /locus_tag="VFD74_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZK48678.1"
                     /translation="VFAPGLLAGTTAPNSGRLSVGTKSPLTGGIKEANSGGQAAQRLG
                     RLRVAAVIVQGSPGDKRYVLHLSKEGARLDDGAPYWGQTNYECAAALRAAYGEKVATI
                     QVGPAGEMGYRNSTVAVTDPEGNPARHAGRGGVGAVMGARGLKAIVVDDAGTERPSIV
                     DKDRFDVARKTLVDGLKSHPVTGQGLPNYGTAILINIINEAGGLPTRNFSLGRFDRAE
                     AISGETVHQNCEDRGGRREHACMTGCVVHCSNVYNDKNGAYVSSGVEYESIWALGANC
                     DNDDIDAIARMDRLCDELGLDTIEMGDTFAVAMEGGAIPWGDAGRMIELFEEVRQGTA
                     LGRQLGNGVVHAGREFGVSRVPAVKGQGIPAYDPRAVKGIGVTYATSTMGADHTSGYA
                     VATNILKVGGDVDPLVAEGQCALSNGLQQATVLFDSAGFCVFLAFAALDQPDSLAAMP
                     EMISAMYGVDFTVNDIGPYGETLLALERDYNRAAGFTAEDDRLPKFFSEERLPPHDVI
                     WDVKDEDLNQVCGWSPVMEF"
     gene            1699..1974
                     /locus_tag="VFD74_03655"
     CDS             1699..1974
                     /locus_tag="VFD74_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZK48679.1"
                     /translation="MVVTVKLGSTLREKAPHFVGGEGTVEIPSGAEGRVIDVMAALGI
                     SEDEVNIIYRNHRLVAPSAHVADGDRVALFPPNFIHFSQFYLKRDTE"
     gene            2110..2340
                     /locus_tag="VFD74_03660"
     CDS             2110..2340
                     /locus_tag="VFD74_03660"
                     /inference="COORDINATES: protein motif:HMM:NF025807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZK48680.1"
                     /translation="MIDVRLFATLPMHSATGRKQFQLEPRAQLTVGEVVDAEGLRRAD
                     IHIIMINGKHASMDSELVDGDRLGLFPPVGGG"
     gene            2351..3532
                     /locus_tag="VFD74_03665"
     CDS             2351..3532
                     /locus_tag="VFD74_03665"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HZK48681.1"
                     /translation="MSALSEGSEARGSAEAADDRFDAAPADLPTKVLVYFTVLIMAVL
                     IAGGLLQLVLAAGNGDAGGIARGAGFGLLGLAVVLLLSARVPTAYRLEEDAAFGTLLV
                     IERRRFAPVRLALSRYETAEGARRVVFPWVPLLSGSRFLGLRGAKVEESLAGFWSFGR
                     DGSRSVIFSGSGRPRTLVSPLDPAAFLHSVGARPDVAHLKGGTLESLGVAGCELTLMR
                     GDITLEVVDAIVNAANSSLLGGGGVDGAIHEAGGPEILAGCQAYRAENGPLPTGKAMA
                     TSAGRLHARRVIHTVGPVWSGGTAGEVALLAEAYTSSLTVAAGEGLRTVSFPSISTGA
                     YGYPLAAAADVALRAVTGFLRAHPGAFDEVRFVLFGDAALAAYRTALARIADDSGQMD
                     R"
     gene            3529..3798
                     /locus_tag="VFD74_03670"
     CDS             3529..3798
                     /locus_tag="VFD74_03670"
                     /inference="COORDINATES: protein motif:HMM:NF025807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine S protein"
                     /protein_id="HZK48682.1"
                     /translation="MTAADPLEIRAFFHLATELGGGGRRSLAHEPGATVASVAAEIGI
                     DAAAVGLVMVNGRKAELDDRLSPGDRVAYFPVYVPYHRVYGMCVL"
     gene            4004..>4908
                     /locus_tag="VFD74_03675"
     CDS             4004..>4908
                     /locus_tag="VFD74_03675"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZK48683.1"
                     /translation="MPVGKKIEGLMERSSWIRKMFEEGGRLRAEHGAENVFDFSLGNP
                     NLEPPAEFHKVLNELIAEPYPGRHGYMSNAGYLETRQAIADYLTEEQGVPVHADNIVM
                     TSGAGGALNVIFKAILDAGDEVLVPRPYFVEYGSYVDNHGGVLRLVDTNPDFSLDLDG
                     ISAAITPKTRAVILNSPNNPTGRIYPEGTVRELGRLLERKGAETGRIIFLIADEPYRK
                     IVYGDTAVPSLMAAYKNTILATSYSKDLSLSGERIGFVAAGSGIRDIQKLMGGLVLAN
                     RILGFVNAPALMQRAVARLQGVHVDL"
BASE COUNT          770 a         1561 c         1718 g          859 t
ORIGIN      
        1 gtcttcgcgc ccggattgct cgccgggacg acggcgccca actcgggtcg gctgtcggtg
       61 gggacgaaga gcccgcttac cggagggatc aaggaagcca attcgggcgg tcaggcggcc
      121 cagcggctgg gccgcctgcg cgtggcggcc gtcatcgtgc aggggagccc aggagacaag
      181 cgttacgtgc tccacctgtc gaaggaaggt gctcgcctcg acgacggtgc tccctactgg
      241 gggcagacca actacgagtg cgccgccgcc ttgcgcgcgg cctacggcga gaaggtggcg
      301 accattcagg tgggccccgc cggcgagatg gggtatcgca attccaccgt cgcagtgact
      361 gaccccgaag gcaatcccgc ccgccatgcc ggcagggggg gcgtgggtgc ggttatgggc
      421 gcgcgtggct tgaaggctat cgtggttgac gatgccggca cggagcggcc ttcgatcgtc
      481 gacaaggacc gcttcgacgt cgctcgcaag accctggtgg atgggctgaa gagccatccg
      541 gtcaccggcc aaggacttcc caactacggc accgcgatcc tgatcaacat catcaacgag
      601 gcgggaggtc tgcccacccg caacttcagc ctgggccgct tcgaccgagc cgaagccatc
      661 tctggcgaga ccgtgcacca gaattgcgaa gaccgcggcg gcaggcgcga acacgcctgc
      721 atgaccggct gcgtcgtgca ctgttccaac gtctacaacg acaagaacgg tgcgtacgtc
      781 tcgagcggcg tcgagtacga gtctatctgg gcgctcggcg ccaactgcga caacgacgat
      841 atcgatgcca tcgcccgcat ggacaggctc tgcgatgagc tgggacttga caccatcgag
      901 atgggcgaca cgttcgcggt cgcgatggag ggcggggcca tcccgtgggg tgacgccggc
      961 cgcatgatcg agttgttcga ggaggtgcgt caggggaccg ctcttggacg ccaactgggc
     1021 aacggcgtgg tgcacgccgg gcgcgagttc ggcgtgtcgc gcgtccccgc ggtgaagggt
     1081 cagggcatcc ccgcgtacga tccgcgtgcg gtcaagggga tcggcgtgac gtacgccacc
     1141 agcacgatgg gtgccgatca cacctccgga tacgcggtgg ccaccaacat cctgaaggta
     1201 ggcggagacg tggatccgct cgtcgccgag ggccagtgcg cgttgtccaa cggtctccag
     1261 caggcgaccg tgctgttcga ctcggccggt ttctgcgtgt tcctggcgtt cgcggcgctc
     1321 gatcagccgg actcgttggc ggcgatgccc gagatgatca gcgccatgta cggcgtcgac
     1381 ttcacggtga acgacatcgg gccctacggc gagacgctct tggcgttgga gcgcgactat
     1441 aaccgggcgg cgggcttcac cgccgaggac gaccgactgc cgaagttctt cagcgaggag
     1501 aggttgccgc cgcacgatgt catctgggac gtcaaggacg aggatctcaa ccaggtgtgc
     1561 gggtggagcc cggtgatgga gttctgacgt gaccctgtgt tcggggaacg tgaagggggg
     1621 cggggcgcga tcgcgccccg cccccgctct ttccgccccg gcaccggggc tgtcctgcgg
     1681 tggtcgcggg gggtcacgat ggtggtgacg gtgaaactcg gatcgacgtt gcgtgagaag
     1741 gcaccccatt tcgtgggcgg cgagggaacg gtggagatcc cgagcggggc cgagggcagg
     1801 gtgatcgatg tgatggcggc tcttgggatc tccgaggacg aggtcaacat catctatcgg
     1861 aaccatcgcc tcgtggcgcc gagcgcgcac gtcgccgatg gcgaccgcgt ggcgctcttc
     1921 cccccgaact tcatccactt ctcgcagttc tatctcaagc gcgacacgga gtgacgtgcg
     1981 ggccgcgtcg cctggctctg ttcgaggcgc ggcggccgcg gccttggcgc gtctgccgcg
     2041 cgtctgcggt ggtagactct tgtcctgcca atatagaggg tcgaggccct cggatcgggg
     2101 atctcttcca tgattgacgt gcgactcttt gcgacgctgc ccatgcactc cgccaccgga
     2161 cgcaagcagt tccagctgga accccgggca cagctcacgg tgggcgaggt ggtcgacgcc
     2221 gagggactgc gccgcgccga tatccacatc atcatgatca acggcaagca cgcgtcgatg
     2281 gactcggaac tcgtcgacgg tgaccgtctt ggtctctttc ctcccgtcgg cggcgggtag
     2341 ctccgtaacg atgtcggcgc tgagcgaagg ctctgaggcg cgtgggagcg ccgaagcggc
     2401 cgacgacagg ttcgatgccg cgcccgccga tctgccgacc aaggtgctcg tgtatttcac
     2461 cgtgctgatc atggcggtgc tgatcgcggg cgggctgctt cagctggtcc tggccgcggg
     2521 gaacggagac gccgggggca tcgcccgcgg cgcggggttc ggtcttctcg ggctggccgt
     2581 ggtcctgctg ctgtctgcgc gtgtccccac ggcctaccgg ctagaggagg acgctgcttt
     2641 cggcacgttg ctcgtgatcg agcgccgtcg tttcgccccc gtgcgcctcg ctctctcccg
     2701 gtacgagacc gctgagggcg cacggcgggt tgtcttcccg tgggtgccac tgctgtccgg
     2761 ttcgcgtttc ttggggcttc gcggggcgaa ggtcgaggag tcgctcgctg gattctggtc
     2821 gttcggtcgc gatgggagtc ggagtgtcat cttctccggt agcggtcggc ctcgcaccct
     2881 tgtctctcct ctcgacccgg ccgccttcct ccattcggtc ggcgcccgtc ccgatgtcgc
     2941 tcacctgaag gggggaacgt tggagtcgct tggcgtggcc gggtgcgaac tcacactgat
     3001 gcgaggtgac atcactctcg aagtggtcga tgccatcgtc aacgccgcga actcctcgtt
     3061 gctcggtggg ggaggagtgg atggcgccat acacgaggcg ggcggcccgg agatcctggc
     3121 gggatgccaa gcctaccggg cggagaacgg tccgctcccg acggggaagg ccatggcgac
     3181 gtcggccggg cgcctgcacg cccggcgggt gatccacacg gttggcccgg tgtggagcgg
     3241 aggcaccgcc ggggaagtcg ccctgctcgc ggaggcatat acgtcaagcc tcaccgtggc
     3301 ggccggcgaa ggtctccgca ccgtctcttt cccctcgata tccaccggcg cctatgggta
     3361 cccgcttgcg gctgccgccg atgtggccct ccgagcggtc accggattcc tgcgggccca
     3421 ccccggggcg ttcgacgagg tgcgcttcgt gttgttcggc gatgcagccc tcgccgccta
     3481 ccggacggct cttgcgagga ttgcggatga tagtgggcag atggatcgat gaccgccgcc
     3541 gaccccctcg agatacgcgc tttcttccat ctggcgactg agctgggtgg cggcggccgc
     3601 aggtcgctcg cgcacgaacc gggcgcgacg gtcgcctcgg tcgcggcgga gatcggcatc
     3661 gacgcggccg ctgtgggact cgtgatggtt aacggccgca aggccgaact ggacgatcgg
     3721 ctgtcgcctg gcgaccgagt ggcctacttc cccgtgtacg ttccgtatca ccgggtctac
     3781 gggatgtgcg tcctgtagtg gtcggcccgc gcggccccgt gcggcgcccg gcggcgagac
     3841 tgtcgggcgc cgcttctccg ggcggaggcc gcactgaacg tcgtccggtg ccaccccgcg
     3901 ttctctgtga ctcgaggacg ggacctctgg tagattggtg tccggcgccc gtcgggcgcc
     3961 ggacactcgt gcgagcgact catgaacgac ttgggaggct atgatgccgg tcggtaagaa
     4021 gatagaaggt ctgatggagc ggtcctcgtg gatccgcaag atgttcgagg aggggggccg
     4081 gctcagggcc gagcacggag ccgagaacgt cttcgacttc agcctgggaa accccaacct
     4141 cgagccgccc gcggagttcc acaaggtgct gaacgagctg atcgcagagc cgtatccggg
     4201 ccgccacggc tacatgtcga acgcgggcta cctcgagacc cggcaggcca tcgccgacta
     4261 tctcaccgaa gagcagggcg tgcctgtcca cgccgacaac atcgtgatga cgtccggcgc
     4321 gggcggcgcg ctgaacgtga tcttcaaagc catcctcgac gcgggtgacg aggtgctcgt
     4381 gcccaggccc tacttcgtgg aatacggttc ctacgtcgac aatcacggtg gcgtcctccg
     4441 cctggtggac accaaccccg acttcagtct cgacctcgac ggcatctcag cggccatcac
     4501 gccgaagacc cgggccgtca ttctgaactc gcccaacaac cccaccggac gcatctaccc
     4561 ggaggggacc gttcgtgagt tgggcaggct cctcgaacgc aaaggggccg agacgggacg
     4621 catcatcttc ctgatcgcgg atgagccgta ccgcaagatc gtgtacgggg acacagcagt
     4681 gccttctctg atggcagcct acaagaacac gattcttgcg accagctact ccaaagacct
     4741 ctccttgtcg ggggagcgca tcggtttcgt ggctgcgggc tcgggcatcc gcgacatcca
     4801 gaagctcatg ggtgggctgg ttctcgcgaa ccgcatcctg ggcttcgtga acgcacccgc
     4861 tctcatgcag cgggcggtgg cgaggctaca gggagtgcac gtggatct
//
LOCUS       DASRMJ010000128         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_154557, whole genome shotgun sequence.
ACCESSION   DASRMJ010000128 DASRMJ010000000
VERSION     DASRMJ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154557"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..659
                     /locus_tag="VFD74_03680"
     CDS             195..659
                     /locus_tag="VFD74_03680"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48684.1"
                     /translation="MIWDELEKAGVPGVQGVWCHEAGGGRMFNIVSIKQAYPGHSRQA
                     ALVASQCHAGAYLGRWVIVVDEDIDPSNTFDVLWAIASRVEPSESIDIIRKCWGGPLD
                     PRSPVGQKNYNSRAIVDACRPFDWKDDFPPVAESSPELREKTLAKWGEVLFG"
     gene            731..1435
                     /locus_tag="VFD74_03685"
     CDS             731..1435
                     /locus_tag="VFD74_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZK48685.1"
                     /translation="MTRHELSEKLVLACHILAAEGHGDMILGHVSVRIPGEERVLMKG
                     AGLGLEEVTEIDLVEIDLDGRVVAGAARRHSEYPIHTEIFRARDDVGAVVHTHPPHAV
                     ALGATGATIEAIGHEGVLFAGAPVFAETTALIRTREEGAAVAACLGRDRAVLLRNHGI
                     VVAGGNIEEAVVYAILLEKAARVQLLAGDAGHRRATASSELTRKVEQIYHERNIQDFF
                     DYYSRRVRGTAPFPHE"
     gene            1582..>2447
                     /locus_tag="VFD74_03690"
     CDS             1582..>2447
                     /locus_tag="VFD74_03690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK48686.1"
                     /translation="MLLIYWVVFFLAVAETGVQLLLPPYLTSTGVAVASVGFLMALLA
                     AGRLGSRLAGAHLYGRESRRRVLLAVLAAVSLSALPFAFSLPAALSGLTLFVHGLSYG
                     LATTMTLALCMERIERPSDAAAIMGWYTGFTSGGHFVGAVVAGYLADRWGVGPSFVAI
                     ALTTGISLPLMAGVKWPDLVKKGRVVVGRGHRVTEVSGSGILGGAEVAEGQRDSAAAT
                     AAPAEGDMESSAGGGRAPRLISLLRTLPAPVYLAVLLAFYINFLNQITNAFYPIFALQ
                     MGLSLTIVGSLKG"
BASE COUNT          375 a          774 c          854 g          444 t
ORIGIN      
        1 cagcgagatc aacccctcac tggtcatgct cagcagctgc aaccaggggc agacgggccc
       61 gcacgcgaag caccccggtt tcggttcgca gctcacgtag ctgtcggggt tcacgcacgt
      121 caccgggttc cccgacggag acccgtgcct gctctacggg ccctacgttc agcaagggcg
      181 tcgtcaagtc gggcatgatc tgggacgagt tggagaaggc cggcgtcccg ggcgtccagg
      241 gagtctggtg ccacgaggcc ggtggcggcc gcatgttcaa catcgtgtcg atcaagcagg
      301 cctacccggg gcactcccgc caggccgcct tggtggcgtc gcagtgtcat gcgggcgcct
      361 acctcggaag atgggtcatc gtcgtcgacg aggacatcga tccgtcgaac accttcgacg
      421 tgttgtgggc catcgcatcc cgcgtggagc cgtcggagtc gatcgacatc atccgcaagt
      481 gctggggcgg gccgctggat ccgcgttcac ccgtggggca gaagaactac aactcccgag
      541 ccatcgtcga cgcgtgccgc cccttcgatt ggaaagacga cttccctcca gtggcggagt
      601 ccagtccgga gctgcgtgag aagacgctcg ccaagtgggg ggaagtcctg ttcggctgat
      661 tcgttagccg ttcgaggcgg cgcttggcga agatgcagtg gacacacggc gaccggtcgg
      721 gggaggccgc atgacccgtc atgagttgag tgagaagctg gttctcgcct gtcacattct
      781 ggcggccgag ggtcacggag acatgatcct cggccacgtg agcgtgcgca tccccggcga
      841 ggagcgcgtc ctgatgaagg gcgccgggct cggtctggaa gaggtgacag agatcgatct
      901 cgtcgagatc gatctcgacg ggcgggttgt ggcaggagcg gcgcgacgcc actccgagta
      961 tccgatacac acggagatct ttcgcgcgcg cgacgacgtg ggcgccgtgg tgcacacgca
     1021 cccgcctcac gcggtggccc taggcgctac cggagctacc atcgaagcca tcggccatga
     1081 aggagtcctc ttcgcgggcg cgccggtgtt cgctgagacg accgcgctca tccgcacgcg
     1141 ggaggaaggc gcggcggtag ctgcctgcct ggggagagat agagcggtcc tgcttcgcaa
     1201 ccatgggatc gtcgtggccg gcggcaacat cgaggaggct gtcgtctacg ccatccttct
     1261 ggagaaggcg gcccgagtgc agctcctcgc cggcgatgcc gggcatcgcc gcgcgacggc
     1321 gtcgtcggaa ctcacccgca aggtggagca gatctaccac gagcgcaaca tccaggactt
     1381 ctttgactac tacagtaggc gggtgcgtgg aacggccccc ttcccgcatg agtgaacccc
     1441 cgcgacgccg acccggatgt ctgggggcgt tgggcgctcc cgcgccgccc atgagggctg
     1501 cagcgcggtc cctgagggct cttgcgccgc ccatgagggc tcccgggccg cccagccgag
     1561 gcgtgtcgag gggctgagcc gatgcttctg atctactggg tcgtgttctt cctggctgtg
     1621 gctgagaccg gggtgcagct cctgctgcct ccgtacctaa cctctaccgg cgtggctgtg
     1681 gccagcgtcg gctttctcat ggcgctgctg gccgccgggc ggctgggttc acgcctggca
     1741 ggggcccatc tctacggtcg cgagagtcgg cgtcgcgtgc tgctggcggt gttggcggcg
     1801 gtctcgctca gcgcgctgcc tttcgccttc tccctgcccg ctgctctttc gggattgacg
     1861 ctcttcgtcc acgggctcag ctacggattg gccaccacga tgacgctggc cttgtgcatg
     1921 gagaggatcg agcggcccag cgacgcggcc gcgatcatgg ggtggtacac gggattcacc
     1981 tcaggcgggc acttcgtggg tgcggtcgtg gccgggtatc tggccgacag gtggggggtg
     2041 ggcccttcgt tcgtggccat cgcgctcacc accgggatct cattgccgtt gatggccgga
     2101 gtcaagtggc cggatctggt gaagaagggg cgcgttgtcg tgggacgcgg ccaccgcgtc
     2161 acggaagtca gtgggagcgg tatcctcggt ggggcggagg tcgctgaagg tcaacgcgac
     2221 tccgcggctg ccacggccgc gccggcggaa ggtgacatgg agtcatcggc gggcggcggt
     2281 cgggctccgc ggctgatcag cctattgcgg actctgcctg cgcccgtcta tctggccgtt
     2341 ctgctggctt tctacatcaa cttcctcaac cagatcacca acgccttcta ccccatattc
     2401 gccctacaga tgggtctctc actgacaatc gtgggctcac tcaaggg
//
LOCUS       DASRMJ010000129         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_46387, whole genome shotgun sequence.
ACCESSION   DASRMJ010000129 DASRMJ010000000
VERSION     DASRMJ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46387"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFD74_03695"
     CDS             <1..539
                     /locus_tag="VFD74_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48687.1"
                     /translation="GRPYVTPLSAAPGGPARGAAGLRVHVVTGGGAGVDLTQELVRLG
                     FSPSLGVVSVFDSDYQAAQELGLMVISAPPFQPLSAESIREHQELIHDADCVVLAPQV
                     YGEGNLADLDVARTALEAGAAVIVVTDPAVETRDLTGGAATALQARLLEAGATAVPDA
                     HAAVMKVSALASLSTKGG"
     gene            546..986
                     /locus_tag="VFD74_03700"
     CDS             546..986
                     /locus_tag="VFD74_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48688.1"
                     /translation="MWVIVHAMSGMALGAVLGSAGVGFPLWGILLASLAAHALLDIVP
                     HWDYTRRRRAWAWALADTLAAALALGLAWLILDLPGYVVWAGLISAAPDLDVLDAVLP
                     FKKHRRLFPSHWKPYPHGKTGSLPGTVIQGLVVTGSVVVLLLAL"
     gene            complement(1037..2266)
                     /locus_tag="VFD74_03705"
     CDS             complement(1037..2266)
                     /locus_tag="VFD74_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48689.1"
                     /translation="MTVGARGDAVSDLIVEEIPLGDPRLEEFVRYPWRLHRGDPFWTP
                     PLRGDLLGNRLLGLTGLMTRAHEYHDEAEVTHFLARRGGEVVGRVSAAINHRFNAHHA
                     CRIGFFGFFDCIDDPDVAGALLDRAKEWVSARGMSAIRGPGEYSNATHERQGVLIDGF
                     DTPPTVEQTHNPPYYATLLEGYGFRKVMDYHAYLVQVNEPAPARLETVAARVEQRRDI
                     TVRDISLKTLEADVRLVVDLYNEAWSHNWGFLPITDGEAASLVDTLRPILDEGLVRFA
                     YVDGEPAAVFGAFPDPYYALRPRWRWYGDSDLVRVARLMLTRRHIPRVRLMFFGVRPA
                     FRRLGIDALLYRDVKRYGETRGYETCDISLLLETNDLILRASEFMGARRYKTWRIYEM
                     PLTPGAPQAESVEQPSG"
     gene            complement(2263..>3289)
                     /gene="purF"
                     /locus_tag="VFD74_03710"
     CDS             complement(2263..>3289)
                     /gene="purF"
                     /locus_tag="VFD74_03710"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZK48690.1"
                     /translation="SSTSDTEIIAALLAEHPAHDVKDALRDVIPQLRGAFSAVVLTER
                     EVIGFRDPYGVRPLVLGRLDDRYVLASETAALDILGATMEREIEPGEMCWIDGDGYHV
                     ERVAAGKGEALCVFEFIYFARPDSVMRGKTLYSARSRMGKELALESPVEADLVIPVPD
                     TGNSAAIGYAAAAGIPFGEGLVKNRYVGRTFINPDDDLRRLGIRMKLNPLSSTIRGRR
                     LVVVDDSIVRGNTTRRLVQMLFDSGAAEVHLRISSPPIVFPCFYGIDMASQGEFIAFE
                     RTVEEVGRELGATSLAYLSLDGLMKATGLGAEGFCAACFSGDYPCPVPEKLRMSKFRY
                     EGSRGRS"
BASE COUNT          517 a         1114 c         1128 g          530 t
ORIGIN      
        1 gtggacgccc ctacgtcacg cctctgtcgg cggctccggg gggaccggcc cgaggtgccg
       61 ccggactgcg cgtgcacgtg gtcacgggcg gcggtgcggg tgtcgatctc acccaggagc
      121 tggtccggct cggcttcagc ccctcactgg gggtggtgag cgtcttcgac tccgactacc
      181 aggcggctca ggagctgggc ctcatggtga tctcagcacc tcccttccag ccgctgagtg
      241 ccgagagcat ccgcgagcat caggagttga tccacgacgc cgactgcgtc gttctggctc
      301 ctcaggtgta cggcgaaggc aacctcgccg acctcgacgt ggcccgcacc gctctcgagg
      361 caggcgccgc cgtcatcgtg gtgacggatc cagccgttga aacccgtgac ctgaccggcg
      421 gggcggccac cgcgctgcag gcccgattgc tcgaagccgg cgcgacggcc gtgcccgacg
      481 cccatgcggc ggtcatgaag gtgagcgcgc tggcctcact atccacgaaa ggcgggtgaa
      541 gccgcatgtg ggtgatcgtg cacgcgatgt cgggcatggc cctcggcgcg gtcttggggt
      601 cggccggagt ggggttcccg ctgtggggca tcctgctcgc ctcgctggcg gcgcacgcgc
      661 tgctcgacat cgtgccccac tgggactaca cgcggcgtcg gcgtgcgtgg gcgtgggctc
      721 tcgccgacac gctggccgcc gcgctggcgc tcggactcgc gtggctgatc ctcgatctac
      781 ccggctacgt cgtctgggcc ggactcatct cggcggcgcc cgacctcgat gtgctcgatg
      841 cggtgcttcc cttcaagaag caccggcgtc tcttccccag tcactggaag ccgtacccgc
      901 acggcaagac gggatctctg ccgggcacgg tgatccaagg gctggtcgtg accgggtcgg
      961 tcgtggtgct gctgctcgcg ctctgaccga cagacggcta cggggccgat ccgccccgac
     1021 gcctcgccgc tgacggctac ccggacggct gttccaccga ctccgcctgc ggcgctccgg
     1081 gggtcagcgg catctcgtag atgcgccagg tcttgtagcg tcgggccccc atgaactcgg
     1141 acgcccgtag gatcaagtcg ttggtctcga gcaacagaga gatgtcgcag gtctcgtagc
     1201 cccgcgtctc cccataccgc ttcacgtcgc ggtagagcag cgcgtcgatg cccagccggc
     1261 ggaacgcggg acgcaccccg aagaacatga gacgcactcg cgggatgtgc cggcgggtga
     1321 gcatgagtcg tgccaccctc acgaggtctg agtcgccgta ccatcgccag cgagggcgca
     1381 gcgcgtagta ggggtcgggg aaggcgccga acaccgccgc gggctcaccg tcgacgtagg
     1441 cgaagcgcac caatccctcg tccagtatgg gacgcagcgt gtccaccaac gaagccgcct
     1501 cgccgtcggt gatgggcagg aacccccagt tgtgggacca cgcctcgttg tagagatcca
     1561 cgaccaggcg cacgtcggct tcgagcgtct tcagcgagat gtcacgcaca gtgatgtctc
     1621 ggcgctgctc cacccgggcg gcaacggtct cgagacgggc cggggccggt tcgttcacct
     1681 gcaccaggta ggcgtggtag tccatcacct tgcggaagcc gtagccctcg agcagggtgg
     1741 cgtagtaggg cggattgtgg gtctgctcga cggtgggagg cgtgtcgaac ccgtcgatga
     1801 gcacgccttg gcgctcgtgg gtggcgttcg aatactcgcc cggcccccgg atggcgctca
     1861 tgcctcgtgc ggacacccac tccttggctc ggtcgagcag ggcgccggcc acgtcagggt
     1921 cgtcgatgca gtcgaagaag ccgaagaagc ctatccggca cgcgtggtgg gcgttgaacc
     1981 ggtggttgat ggcggcggac accctgccca ccacctcccc gccccggcgc gccaggaagt
     2041 gcgtgacctc cgcctcgtcg tggtattcgt gggcgcgggt catgagtccg gtgaggccca
     2101 ggagacggtt gcccaggagg tcgccgcgca gcggaggtgt ccagaaggga tcgccccgat
     2161 ggagccgcca ggggtagcgc acgaactcct ccagccgcgg atcacccagc gggatctcct
     2221 ccacgatcag atcagagacc gcgtcgcctc tggccccgac ggtcacgacc gtccccgcga
     2281 gccttcatac cggaacttgg acatgcgcag cttctccggg accgggcacg ggtagtctcc
     2341 gctgaagcac gccgcgcaga acccttcggc cccgagaccc gtggccttca tcaatccatc
     2401 gagggagaga tacgccagcg acgtcgcgcc caattcgcgc cctacttcct ccacggtgcg
     2461 ctcgaacgct atgaactccc cttgactcgc catgtcgatg ccgtagaaac acggaaacac
     2521 gatgggcggt gacgagatgc gcaggtgcac ctcggccgcg ccggaatcga atagcatctg
     2581 caccaaccga cgcgtcgtgt tgcctcggac gatggagtca tcgaccacta ccaggcgcct
     2641 cccgcggatg gtggagctga gagggttgag cttcatgcgg atgcccaggc gacgcaggtc
     2701 gtcgtcgggg ttgatgaacg tgcgccccac ataacggttc ttcaccagcc cttcgccgaa
     2761 ggggatgccg gcggcggcgg cgtagccgat ggcggccgag ttgcccgtgt cgggcaccgg
     2821 gatgacaagg tcggcctcca cgggagactc gagggcgagt tccttcccca tcctgctgcg
     2881 ggccgagtag agggtcttgc cccgcatcac ggagtcgggg cgggcgaagt agatgaactc
     2941 gaagacgcag agcgcttcgc ccttgccggc cgcaacgcgc tccacgtgat agccgtcgcc
     3001 gtcgatccag cacatctcgc ccggctcgat ctcccgctcc atcgtcgcac ccaggatgtc
     3061 gagagccgcc gtctcactcg ccagtacgta gcgatcgtcc aggcggccca ggacgagagg
     3121 ccgcaccccg taggggtcgc gaaaaccgat gacctcgcgc tcggtgagga cgacggcgct
     3181 gaaggcgccc ctcagctggg ggatgacgtc ccgcagggcg tccttgacgt cgtgcgccgg
     3241 gtgctcggcc aacagcgccg cgatgatctc ggtgtcggag gtggaggac
//
LOCUS       DASRMJ010000130         6236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_299651, whole genome shotgun sequence.
ACCESSION   DASRMJ010000130 DASRMJ010000000
VERSION     DASRMJ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299651"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VFD74_03715"
     CDS             complement(<1..971)
                     /locus_tag="VFD74_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZK48691.1"
                     /translation="MALMEIIECVDQLGTEMVRRVPESGSGEFKIGSQLIVRENQWAV
                     FFRDGKALDTFGPGRHTLSTQNIPFITALITTPLFGDTPFRAEVFFVNRAVFIDQKWG
                     TAEPVLFRDSEFKMVRLRANGIYSLQVVDPKLLVLKLVGTRSIYTTGQIEDFLRGIIV
                     GRLSDLLGESLDSVLDLPRYYDELGAGLKARVKDDFYQYGIALVDFVVNAITPPEDVL
                     KRIDERSGMEAIGGMDRYFQFKAAQAIGDIAKGGGGGGMGGEGGGGSGIGDAAATGVG
                     LGAGAGLGFMIPGMMQQAMQSGAQPKMRCPGCSKDIPFGSKFCPECGA"
     gene            complement(978..1688)
                     /locus_tag="VFD74_03720"
     CDS             complement(978..1688)
                     /locus_tag="VFD74_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48692.1"
                     /translation="MDEKANESVSSAEAAAESAARTAGESAAERAAEAAVQPAGTGPD
                     GVGAQTAGEAAAGSASEAVAAPDGVGARIEESKNWFERAAEHIPGYKGYKEKELRREA
                     DKLLREYVAERIEAARARLESVALAVTDRGELQLLAGVDRTGRKLRTIRDKYLFADYG
                     YSGIFDTVKVGDEILDRLYRFDTQSQEDALRLEQLAATLSADSPSLRSDLGLLDQRVT
                     ALDQSFNEREHVITGVGR"
     gene            complement(1761..2411)
                     /locus_tag="VFD74_03725"
     CDS             complement(1761..2411)
                     /locus_tag="VFD74_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48693.1"
                     /translation="MYPCRACGKAFQVATDGLDEIRPLTADLTTELAVPPDVRYLAVW
                     RFLAAVDVRETRSGSGAEPSQVWQGIRRLAAPEPPFLYVPAFVFSRAVVQQLGLALVQ
                     AQPRLDLHEGLPREAPPGLRTANENGAVSEEDWAPEAGFGTLSPVLLTRDDAEVVAHF
                     VYLALESRSIPDLRSIDYDLALTGGDLLFLPAVYDVRQVRDSNWRLLLREFDGLVA"
     gene            complement(2473..3330)
                     /locus_tag="VFD74_03730"
     CDS             complement(2473..3330)
                     /locus_tag="VFD74_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48694.1"
                     /translation="MSRGGFERRFFPAKIEKLSAVGKAALWLREHQDTPDDIAESFFT
                     EARLLYVPIWEVRAYVVGWEFGKKLRTKAELVQVGREEHVQTRLVDEGVEEGYFSERR
                     LYREAADLSALGMGRPHITGREFTLPYLVGELEQEATVLEADRELESVREQARESFLR
                     PPTGTLSRQTQLFLLRESACLIYYPLWSLCYRYRGRAYEMTVDGRNGLVHSARAPADN
                     RARLAALLASYAGLAVGLALSVSAWESWGELREPAAYAALAVTVAASGVYWRFRLVRE
                     VEHYESFSA"
     gene            complement(3473..4273)
                     /locus_tag="VFD74_03735"
     CDS             complement(3473..4273)
                     /locus_tag="VFD74_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48695.1"
                     /translation="MIETAETVCPQCGALLTAPHQVDTFSCSYCGSSLRMGDGLRLYR
                     LVERPLVSREAAAGFLRAWFAGNEGPADLETAARYELGGLRYFPFLRTRRNGADRVAP
                     LAPLPSPEVMSLANVPAQLVAAGRAPSDVSREISGEAAGQSAADVPPEWAPLDEGLLR
                     DGVRRASADPEAAELLIEQRAYYPVHYSYQGEHYSALVDAGAGRVLVARRPARREVLG
                     ERKVALGALALLFGEAVLVPGLAARIVVVVATAAGLYPLLRWALAHHG"
     gene            complement(4405..5535)
                     /locus_tag="VFD74_03740"
     CDS             complement(4405..5535)
                     /locus_tag="VFD74_03740"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48696.1"
                     /translation="MIDRDTIRSLASSRSDDGVFVSAFFTTSRLDDWRQTAPTFLNSG
                     FNGLLRERPLGKEPKKRLQEDVERIQEVLRYEIGPRTEGLAVFADAESGRFESIELPF
                     RFSNKLIVEPTAYLRPLIHALALLEPFVLARVSRDDGMIYSIDEWRLTRTEGHSGPYL
                     QSTDRDTGDVPVKEYYAAARQESLVDHHFKELAAALDRTVDEIGASHVVLSGQHDIVS
                     NFRKALSARAAAMVVAEIPLDHAASTAQLLVGARAALADARHVATGALAERIAEGMGA
                     GGRGVGGFDDTLAALRRGQVQTLLIDRAYRPAGWACGSCGHVLLAPTPACPVCDGGTV
                     PIVDALGEAVRSAILQGAYVEVGEDLPVLDSLEGIAGLLRYG"
     gene            complement(5605..5907)
                     /locus_tag="VFD74_03745"
     CDS             complement(5605..5907)
                     /locus_tag="VFD74_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48697.1"
                     /translation="MADKKVDRNVRSSDQERPSTPTQKMTGSLSDSGTVNIAQADRDK
                     GKSALFDRFRDRDARYRQEQSFADLAAQAVRTMPAAVASSTGDAPEDEASEDEPPA"
     gene            complement(6050..>6236)
                     /locus_tag="VFD74_03750"
     CDS             complement(6050..>6236)
                     /locus_tag="VFD74_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48698.1"
                     /translation="CWGVGCGGVIAPGSISSGGVCAFSFIVLLAFLPSPSADDTSPDR
                     AVFHHGRAKVVLPAGLR"
BASE COUNT         1085 a         2192 c         2029 g          930 t
ORIGIN      
        1 gcgccgcact ccgggcagaa cttgctgccg aaggggatat ccttcgagca gccggggcag
       61 cgcatcttcg gctgggcgcc cgactgcata gcctgctgca tcatgcccgg gatcatgaag
      121 ccgaggccgg cgccggctcc gaggcccaca ccggtggccg ccgcatcccc gatgcccgag
      181 ccgccaccgc cttcgccgcc catgccaccg ccgccaccgc ccttcgcgat gtcgccgatg
      241 gcctgcgccg ccttgaactg gaagtagcgg tccatcccgc cgatcgcttc cataccggaa
      301 cgctcgtcga tgcgcttgag aacgtcttcg ggcggcgtga tcgcgttgac cacgaagtcg
      361 accagggcga tgccgtactg gtagaagtcg tccttgaccc gggccttgag accggctccc
      421 agttcatcgt agtagcgagg aaggtcgagc acggagtcaa gactctcgcc caggaggtcg
      481 gagagtcggc cgacgatgat gccgcggagg aagtcctcga tctggccggt ggtgtagatg
      541 gagcgcgtgc ccaccagctt caacacgaga agcttgggat cgaccacctg aagagaatag
      601 atcccgttgg cgcgcagccg caccatcttg aactctgaat cccggaagag taccggctcc
      661 gccgtccccc acttctgatc gatgaagacg gccctgttga cgaagaagac ctcggcccgg
      721 aagggagtgt cgccgaacaa gggggtcgtg atcagagcgg tgatgaaggg gatgttctgg
      781 gttgagagcg tgtgacgacc cggaccgaac gtgtcgagcg ccttgccgtc gcggaagaac
      841 accgcccact ggttctcgcg tacgatcagc tgcgacccga tcttgaactc ccccgaaccc
      901 gactccggca cccggcgtac catctcagtg ccgagttgat ccacacactc gatgatctcc
      961 atcaaagcca tcttcggtta cctccccacg ccggtgatga cgtgctcgcg ttcattgaag
     1021 ctctggtcga gcgccgtcac cctctgatcg agaaggccga ggtcgctcct gagagaggga
     1081 ctgtccgccg acagggtcgc cgccaactgc tcgaggcgca gcgcgtcctc ctggctctgg
     1141 gtatcgaacc ggtagaggcg atccaggatt tcgtcgccca ccttgaccgt atcgaagatg
     1201 cccgagtacc catagtccgc gaacaggtac ttgtccctga tggtgcggag cttgcggccg
     1261 gtgcggtcca cgccggcgag gagctgtagt tcaccgcggt ccgtcaccgc cagcgcgacc
     1321 gactccagcc tggcgcgcgc ggcctcgatc cgctcggcca cgtactcgcg tagcagcttg
     1381 tccgcctcgc ggcggagctc cttctccttg tagcccttgt acccggggat gtgctcggcg
     1441 gcgcgctcaa accagttctt gctctcctcg atgcgggcgc cgacgccgtc aggagccgcc
     1501 actgcttccg aggcgctccc cgcggcggcc tcgccggcag tctgggcgcc gacgccgtcg
     1561 gggcccgtgc cggcgggctg gacggccgcc tccgccgctc tctctgcggc ggactcacca
     1621 gccgtccggg cggcactctc tgcggccgcc tccgcggaac tcacgctctc attggccttc
     1681 tcgtccacga ccactccttc ccgggaactc ccccgattgc atgtgacacg acaaacgtcg
     1741 gcgatcaggc agatcgccga tcaggccacc aggccgtcga actcccgcag cagcaaccgc
     1801 cagttggagt cgcgcacctg ccgaacatca tagacggccg gcaggaacaa cagatccccc
     1861 ccggtgagcg ccaaatcata gtctatggat ctgagatcgg ggatggagcg cgattccagc
     1921 gcgaggtaga cgaagtgcgc cacgacttcc gcgtcgtctc gagtgaggag caccggcgag
     1981 agcgtgccga agccggcctc gggagcccag tcttcctcgg agacggcgcc gttctcgttc
     2041 gccgtcctga gacccggcgg cgcctctcgg ggcagcccct cgtggaggtc gagacgcggt
     2101 tgcgcttgga ccaaggcgag ccccagctgc tgcaccaccg cccggctgaa cacgaaggcg
     2161 ggaacgtaca ggaacggcgg ttcgggggcg gccagtcgcc ggatgccctg ccacacctgc
     2221 gagggttcgg cgccgcttcc cgaccgcgtc tcccgcacgt cgacagcggc caagaaccgc
     2281 cacacagcca agtagcgcac atccgggggt acggccaact cggtcgtcag gtcggctgtg
     2341 agtggccgga tctcgtccaa tccgtcggta gcgacctgga aggccttgcc gcaggcacgg
     2401 catggataca ccgcagcgtc gcgctgcgcg tccagcggac caccgcaatt cgaacagaca
     2461 agggccagca gatcaggcgg agaaggactc gtaatgctcg acctccctga ctagccggaa
     2521 gcgccagtaa acgccggaag ccgcgaccgt gacggccagc gccgcatacg ccgcaggctc
     2581 gcgcagctct ccccaagact cccatgccga caccgagagg gcaagaccca ccgccagacc
     2641 ggcgtatgaa gccagcaacg ccgcgaggcg cgcgcggttg tcggccggcg cgcgggccga
     2701 gtgcaccagg ccgttccggc cgtccacggt catctcgtaa gcgcgccccc gataccgata
     2761 gcacagcgac cacagcggat agtagatgag acaggcggac tcccggagaa ggaagagctg
     2821 cgtctgacgg gagagcgtgc cggtcggcgg ccgaaggaac gattctcgcg cctgctcgcg
     2881 caccgattcc agttcacgat ccgcctcgag aacggttgct tcctgctcca actcgcccac
     2941 gaggtagggc agggtgaact ccctgccggt gatgtgcggc cggcccatgc cgagcgccga
     3001 caggtcggcc gcttctcgat agagccggcg ctcgctgaag tagccctcct ccaccccctc
     3061 gtccacgagc cgggtctgaa catgctcctc ccgacccacc tggacgagct cggccttggt
     3121 ccggagcttc ttgccgaact cccagcccac gacgtacgcc cgtacttccc agatggggac
     3181 atagagcaga cgggcctcgg tgaagaacga ctcggcgatg tcatcgggcg tgtcctggtg
     3241 ctccctaagc cacagcgccg ccttgcccac cgcgctcagc ttctcgatct tggccgggaa
     3301 gaagcggcgt tcgaagcctc cgcgcgacat ggcccggaac ggtgtcccgc agcgaccgca
     3361 cgaaatcagg cggtcaccgt cgcgcgccga gaggggcccg gcgcagcgcg gacacgagat
     3421 ggagcggaga gtcgcgcggc gggtgggcgg ctccaccggt gcacccacgg gctcacccat
     3481 gatgagccag cgcccaacga aggagcgggt agagccccgc cgccgtggcc accaccacga
     3541 cgatccgggc cgcgagccca ggcacgagga cggcctcccc gaagagcagc gccagggccc
     3601 ccaaggcgac cttgcgctcg cccaacacct cgcgccgggc gggccgacgc gccaccaaca
     3661 cgcgaccggc ccccgcatcg acgagcgccg agtagtgctc accttggtag ctatagtgca
     3721 ccggatagta ggcgcgctgc tcgatgagca gctccgccgc ctcggggtcg gcggaggcgc
     3781 gcctcacccc gtcgcggagc aacccttcgt ccagaggggc ccattccggt ggaacgtccg
     3841 cggcggactg cccggcggcc tccccggaga tctcccggga gacatcggac ggcgcgcgcc
     3901 ccgccgcgac caactgcgcc ggcacattcg ccagagacat cacctcgggg ctcggcagcg
     3961 gcgccaacgg agccacccgg tcggcgccgt tgcgcctcgt ccggaggaac gggaagtagc
     4021 gcagcccccc gagctcgtat cgggccgcgg tctccaggtc cgccggaccc tcgttcccgg
     4081 cgaaccaggc gcgaaggaag cctgctgcgg cctcccggct caccagcggc cgctccacca
     4141 gccggtacag gcgcaacccg tcacccatgc gtaggctgga cccgcagtag gagcaggaga
     4201 aggtgtccac ctggtgaggc gcggtgagga gagcgccgca ttgggggcag acggtctcgg
     4261 ccgtctcgat catgatcgtg caccgttcat ggtgccattg tatcggcgga gacgcccccg
     4321 agcccagaga agccggtggc tccgcgggca gcgcgacaga tcactcggcg cagacaggcc
     4381 cacgcaacat acgcgtggct cggatcaccc gtagcgaagc aggccggcga taccctcgag
     4441 cgaatccagg acgggaaggt cctctccgac ttcgacgtag gcaccctgaa ggatcgccga
     4501 gcgaaccgcc tctcccagcg cgtcgactat tggcacggtc ccaccgtcgc acaccgggca
     4561 ggcgggcgtc ggcgccagca gcacgtgacc gcacgacccg caggcccagc cggcgggtcg
     4621 ataggcgcgg tctatcagaa gagtctgcac ctggcccctg cgcaaggcag cgagcgtatc
     4681 gtcgaagcca ccgactcccc tacctcccgc gcccatccct tcggcgatgc gctcggccag
     4741 cgccccggtg gccacgtgcc gggcgtcggc gagggccgcg cgcgcaccca ccaggagctg
     4801 tgccgtagac gcagcgtggt ccagaggtat ctccgccacg accatggccg ccgcgcgcgc
     4861 gctcagggcc ttcctgaagt tgctcacgat gtcatgttgg ccggagagca cgacgtgcga
     4921 tgccccgatc tcgtccacgg tccgatccag cgcggcggcc aactccttga agtggtggtc
     4981 caccagggac tcctgacgcg cggccgcgta gtactccttc acgggcacgt ccccggtatc
     5041 ccggtcggtg gactgcaggt agggcccgct gtggccctcc gtccgcgtaa gcctccactc
     5101 gtcgatgctg tagatcatgc cgtcgtcgcg gctcacccgc gccaacacga aaggctcgag
     5161 cagcgcgagg gcgtggatca gcggacgaag gtatgcggtc ggctccacga tcagcttgtt
     5221 cgagaaccgg aacggcagct cgatcgactc gaagcgcccg ctctctgcgt ccgcgaacac
     5281 ggcgagaccc tcggtacgcg gccctatctc gtaacgcagg acctcctgta tgcgctcgac
     5341 gtcctcctgc aggcgcttct tcggctcctt gcccaggggg cgttccctga gcaggccgtt
     5401 gaacccgctg ttcaggaagg tgggtgcggt ctgacgccaa tcgtcgagcc tgctggtggt
     5461 gaagaaagcc gacacgaaga cgccgtcgtc actgcggctc gatgcgagac tgcggatggt
     5521 atccctatcg atcatgacta ccccctcggg ctcgggccgg gcgcggggaa cccgcgccgc
     5581 gcggaaagga ctgagcagct cggactacgc tgggggttcg tcctctgatg cctcgtcctc
     5641 cggggcatcg cccgtactgg aagcgaccgc cgcgggcatc gtccgaacgg cctgcgccgc
     5701 caggtccgcg aaggactgct cctgccggta gcgcgcgtcg cgatcgcgga atcgatcgaa
     5761 gagtgctgat ttgcctttgt cccgatccgc ctgagcgatg ttcacggtgc cggagtcgga
     5821 gagagagccc gtcatcttct gagtgggtgt ggagggacgc tcctggtccg acgaacggac
     5881 gttccgatcg accttcttgt ccgccatgtt tcctccttgc ctggccaatc ccggggcgat
     5941 tacagcggaa ccagcgttgc ggttcgacgt tcggtggaat aagttggtac ccgagtgtct
     6001 acagggacaa acggcgggag cgacctccga ggatgcgcgg ctcgggtcct caccggaggc
     6061 ccgccggaag aacgaccttg gcgcggccgt ggtgaaacac cgcacggtcg ggcgacgtat
     6121 cgtcggcaga cggagatgga aggaaagcga ggaggacgat gaacgagaac gcgcacacac
     6181 cacccgagct tatggatccg ggcgcgatca cgccgccgca gccaacaccc caacac
//
LOCUS       DASRMJ010000131         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_413006, whole genome shotgun sequence.
ACCESSION   DASRMJ010000131 DASRMJ010000000
VERSION     DASRMJ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413006"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VFD74_03755"
     CDS             complement(<1..857)
                     /locus_tag="VFD74_03755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZK48699.1"
                     /translation="MVRELSDVVFVSAVRTPMGRFGGTLKDTKVYDLASFAIREALRR
                     AGVAGEDVDDAIVASCRQAGNYVNPGRTAALKGGCGATTPGYTINKACPAGMKAVSLA
                     TQLIQVGQADIALCGGMESMSTIPHLVRGYRWQGFRMGNVQLEDGWSDSHDPVADMAM
                     GDTAENLVDKYGLTRQELDEFAVASHLKASAAQSNGWFDEEITPVIIPAKGKQPEVVF
                     DKDESIRHDADLAAMGKLKPAFRPDGQVTAGNSCGLTDGAAFMMAMSRDEAHRRGLEP
                     LFSVVDYTQT"
     gene            complement(1098..1610)
                     /locus_tag="VFD74_03760"
     CDS             complement(1098..1610)
                     /locus_tag="VFD74_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HZK48700.1"
                     /translation="MLHQRMQEAINRQVNAELYSAYLYLSMSARFAALGLPGGANWMR
                     VQVQEELFHASRFYDSMVERGGAVQLSGIEQPPGAWLSALDMFEEALGHEQKVTALIY
                     GLADLALELKDHASHNMLQWFIAEQVEEEAAADQMIHKLRLTQDAPGGLFQLDNEMGL
                     RVYLPPVVAP"
     gene            1825..2808
                     /locus_tag="VFD74_03765"
     CDS             1825..2808
                     /locus_tag="VFD74_03765"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZK48701.1"
                     /translation="MRIAIVGPGSLGSVYAALLSRAGHDVTLMGRAERVAILRSRGVV
                     LRGLEELHASVPATDDPASVGPVDLLLLCTKAIDTKTTLAALADMKVGAAASLQNGIV
                     KESLLADVFGRDRVLGAATMVGAERRSDGTVDFTARGTTYLGEFDGRISDRLGTIVAA
                     LDDAGLPAQALTDIESVIWSKACLSAGAFAVSVFSRLPVTSILAQAELAGATADLITE
                     AAGIAAAAGHKVHDYPGMRVASWARDPRHVVLEDMAQRAAEMTVASRVVRVSMLQDLL
                     AGRPMEVDEVYGGLLAQAELTATPAPTLALVYRLLKGIDSASRAAGIAARE"
     gene            2918..4198
                     /locus_tag="VFD74_03770"
     CDS             2918..4198
                     /locus_tag="VFD74_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK48702.1"
                     /translation="MEATEDTTDTPPPHPRRFVGERLQLGVICAATFVVWAGFGAILP
                     YLPVFLQEEAAASIRYIGVIAAAYYVGTFAFSALLGGLSDRIGRKPVLVAGVWVYGLA
                     TLLFLTTTHPVWFILFRFLEGAGAAAVYPAGQAFVADITPAHRRSQAYGWMNTAQFGG
                     LVAGPALAWPLYQLGGGHGLWSFYSIFLFGALLALTTGAALLLLLREPERRLSAEEPA
                     TARPPYRRLLTRPVIAFMVVASAVHLAIGSWEVIWSIWLRELGASMSFVGLTWMLFSL
                     PMLFSFVGGYFADRHSRFLLMFSGYIISAATWIVYGTTHTLTVLLVFSVVEGVAMAWA
                     NPAKQAFLVQVSPARWVGSVQGLEVTAAHLAALVGTLAAPFLFEIMSGRVIALGGVIA
                     LVALFAVIPVLGPEWKRLSAPNLARGEVEASSGA"
     gene            complement(4252..5298)
                     /locus_tag="VFD74_03775"
     CDS             complement(4252..5298)
                     /locus_tag="VFD74_03775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HZK48703.1"
                     /translation="MMLMSERGSFRALVLREGGEEEPEVAVEPVEFSDLPEGDVVVEV
                     AFSSLNYKDALAVTGAGRVVRSYPMVPGIDLAGTVVRSAAADYRIGQQVILTGWGIGE
                     SHWGGFAEFARVRSEGLVPLPEGLSPMHAMALGTAGLTAMLAVIALEDAGIVATEEEW
                     TDRPGVLVTGASGGVGSIAILLLAGAGHRVVASSGRHESAPYLHLLGAEEIIERADLE
                     APARSLESQRWKAAVDTVGGATLARVLAETSYGGAVAACGLVGGAELHTTVMPFILRA
                     VRLLGIDSVSAPSAVRTEAWERLAREVRPEAIESLTRVIGLEEVLGHARQMVGGRGRG
                     RTVVDPRTAATTAE"
     gene            complement(5298..>5732)
                     /locus_tag="VFD74_03780"
     CDS             complement(5298..>5732)
                     /locus_tag="VFD74_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48704.1"
                     /translation="HLPELTASLAGLGTWEHRQFVEAASRVRGGRYRVALEDLDLPAL
                     WESLLEKRPLLVRILENPTLQEHETFTDLLRATFHLTEELAVHADLSGISQKDRAHLS
                     GDASRVYGLLVPSWLAYVGHLRDEYPYLFSLAARTNPFDGEV"
BASE COUNT          899 a         2029 c         1828 g          976 t
ORIGIN      
        1 gtctgcgtgt agtcgaccac cgagaagagg ggctccagtc cgcgcctgtg agcctcgtcg
       61 cggctcatcg ccatcatgaa ggcggcgccg tcggtgagcc cgcaggagtt gcctgcggtc
      121 acctggccgt cgggccggaa cgcgggcttc agtttcccca tcgcagcgag gtccgcgtcg
      181 tggcggatgg attcgtcctt gtcgaagacg acctcgggct gcttgccctt ggcggggatg
      241 atcaccgggg tgatctcctc gtcgaaccac ccgttgctct gcgcagccga ggccttgaga
      301 tgactcgcga ccgcgaactc gtcgagctct tgacgggtga ggccatactt gtccaccagg
      361 ttctccgcgg tgtcccccat ggccatgtcg gccactggat cgtgagagtc ggaccagccg
      421 tcctccaact ggacgttccc catgcggaag ccctgccagc ggtagccacg caccagatgc
      481 gggatcgtgg acatgctctc catcccgccg cagagcgcga tgtcggcctg gcccacctgg
      541 atgagttggg tggcgaggct tacggccttc atgccggcgg ggcaggcctt gttgatggtg
      601 tatccggggg tggtggcgcc gcagccgccc ttcagcgcgg cggtgcgccc ggggttcacg
      661 tagttgcccg cttggcggca cgaggcgacg atcgcgtcgt cgacgtcttc gcccgcgacg
      721 cccgcgcgac gcagcgcctc cctgatcgcg aaagaagcta gatcgtaaac cttcgtgtcc
      781 ttgagcgtgc ctccgaagcg ccccatcggc gtgcggaccg cgctcacaaa gaccacgtca
      841 ctcaactctc gcaccacgcg cgtactccct ccgttcggcg cccgcactca gacggcgggc
      901 gttgtctttc gcggtagtcg cgccgagggc cgcatgatcg gccaccctcg actccaggac
      961 gagccattgg ccgggccggc cgactgactg gccgggcgac caagaaactg ccgggctgat
     1021 gatagcagga atcacaggcc cgaggtagca gcggggcggg cccgctgaca ggcgcgccca
     1081 cgaccgagta tttcagttca aggcgcgacg accggcggta ggtagacccg caagcccatc
     1141 tcgttgtcaa gctggaagag ccctccggga gcatcctgcg tgagtcgcaa cttgtggatc
     1201 atctgatcgg ccgccgcctc ctcttccacc tgctctgcga tgaaccactg cagcatgttg
     1261 tgggaggcgt gatccttcag ttcgagagcc aggtcggcca ggccatatat gagcgccgtg
     1321 accttctgct cgtggccgag ggcctcttcg aacatgtcca gagccgacaa ccaggcaccg
     1381 ggtggctgct cgatcccgga gagttgcacc gcgcctcccc gctcgaccat ggagtcgtag
     1441 aaccgagagg cgtggaacaa ctcttcctgg acctggaccc gcatccagtt cgcgcctccc
     1501 ggcaaaccga gggccgcgaa ccgagccgac atagaaagat acaggtacgc cgaatacagc
     1561 tcggcgttga cctggcgatt gatcgcttcc tgcatgcgct ggtgaagcat cacattcact
     1621 ccttcacttc gaacttccgc tccgaacggg cgacgcagac gggtccgcac ggagcccttc
     1681 tgctccggca tgctgcccgc cgatgccgtc tgcgaaacgc gggacaacga ggtatgctgg
     1741 tccgaggccg accaagacca ctcgccaacc tgagcttgcg ttggaatcgt gcgcgagacc
     1801 ccggcggact gaagggacag aggcatgcgc atcgccatcg tcggaccagg atcgctcgga
     1861 tcggtgtacg cagctctgct ctcgcgcgca ggacacgacg ttacgctcat ggggcgtgcc
     1921 gagcgcgtcg ccatcctccg cagccgagga gtggtgctcc gcggcctcga ggagctgcac
     1981 gcgagcgtgc ccgcgaccga cgaccccgcg agcgtcggcc ccgtggacct actcctgctt
     2041 tgcaccaaag ccatcgacac caagaccact ttggccgccc tcgcggacat gaaggtgggc
     2101 gcggccgcga gtctgcagaa cgggatcgtc aaggagtcgc tgctcgcgga cgtcttcgga
     2161 cgcgaccgtg tcctgggagc cgcgaccatg gtgggagccg agcgccgctc tgacggcacc
     2221 gtggatttca ccgcccgggg caccacctat ctcggcgagt tcgacggccg catatccgac
     2281 cgtctcggga cgatcgtggc cgcactcgac gacgcgggac ttccggcgca ggccctcaca
     2341 gacatcgaat ccgtcatctg gtccaaggcg tgcctctcgg ccggggcttt tgccgtgtcg
     2401 gtgttctcgc gccttccggt cacctcgatc ctggcccaag ccgagctggc cggcgccacg
     2461 gccgacctca tcaccgaggc cgccggcatc gccgcggcgg ccgggcacaa ggtgcacgac
     2521 taccccggga tgcgggtggc ctcctgggcg cgcgaccccc gtcacgtcgt cctcgaggac
     2581 atggctcagc gcgccgcgga gatgacggtc gcatctcgag tggtgagagt gtcgatgctc
     2641 caggatcttc tcgccggcag gcccatggaa gtggacgagg tgtacggagg ccttctcgcg
     2701 caggcggaac tgaccgcgac cccggcgccg acgcttgctc tcgtctaccg gctgctcaag
     2761 ggtatcgaca gcgcgagcag ggccgccggc atcgccgccc gcgagtagcg cctgccgcgc
     2821 tcgccttcga gcctccgccc gcgaccggcg actgggcccg agcgccccca agcaggagca
     2881 gcggccgccg tacatgtgcg gtatcctact cagccgcatg gaagccaccg aagacacaac
     2941 cgatactccg ccgccgcacc cccgacgctt cgtcggcgag cgactgcagc ttggggtcat
     3001 ctgcgccgcc accttcgtgg tctgggcggg cttcggagcc atcctcccct accttccggt
     3061 attcctgcag gaggaggccg ccgcctccat ccgctacatc ggcgtcatag cggcggccta
     3121 ctacgtaggc acgttcgcct tctcggcgct cctcgggggt ctgagcgacc ggatcggccg
     3181 caagccggtg cttgtcgcgg gagtgtgggt ctacggtctc gccactctgc tcttcctcac
     3241 gaccacgcac ccggtgtggt tcatcctctt ccgcttcctc gagggcgccg gcgcggccgc
     3301 ggtgtatccg gcgggccaag ccttcgtggc cgacatcact cccgcccatc gccgcagtca
     3361 ggcctacggc tggatgaaca ccgcgcagtt cggcggactc gtggccggcc ccgccttggc
     3421 gtggcccctc tatcagctgg gtggaggtca cggtctctgg tccttctact cgatcttcct
     3481 cttcggagcg ctcctcgccc tcacgacggg cgctgctctg ctcctcctcc tgcgggaacc
     3541 ggagcgccga ctctcggcgg aggagcccgc taccgctcgc cccccctacc gccgcctcct
     3601 cacgcggccg gtcattgcct tcatggtcgt cgcctcggcc gttcacctcg ccatcggctc
     3661 ctgggaagtg atctggagca tctggttgcg cgagctcggc gcctccatgt cgttcgtggg
     3721 tctcacctgg atgctcttca gcctcccgat gctgttctca ttcgtgggag gctacttcgc
     3781 cgaccgccac agccgtttct tgctcatgtt cagcggctac atcatttccg cagccacgtg
     3841 gatcgtctac gggaccacac acaccctcac ggtgctactc gtcttctcag tggtggaggg
     3901 ggtggcgatg gcttgggcca atcctgccaa gcaggcgttc ttggtccagg tgagtcccgc
     3961 gcgctgggtg gggagcgtac aagggctgga ggtgaccgcg gcccatctcg cggcgctggt
     4021 gggcacactt gccgcaccct ttctgttcga gatcatgtcg ggacgggtga tcgctctcgg
     4081 aggagtgata gcactcgtcg cgctcttcgc cgtcatcccg gttctcggac ccgagtggaa
     4141 gaggctctca gcgcccaacc tggcgcgtgg cgaggtcgag gcctcgagcg gagcgtagcc
     4201 gtcgggactc caacggtgtc gcctgctcct ccccaccgct tccgtcccgg gctactccgc
     4261 ggtcgtcgcc gccgtcctcg gatcgaccac ggtccggccg cgaccacggc ctcccaccat
     4321 ctggcgggca tgcccgagca cttcctcgag gccgatgacc ctggtgagag actcgatggc
     4381 ctcgggcctg acctcacgcg ccaggcgctc ccaagcctcg gtgcgcacag ctgagggggc
     4441 tgagaccgaa tcgatgccga gcagccggac ggccctcagg atgaatggca tcacggtagt
     4501 gtgcagctcg gccccaccca ccagtccaca cgccgcgacc gcgcccccgt aactcgtctc
     4561 ggcgagcacg cgggccaggg tggcgccccc cacggtgtcg acggccgcct tccatctctg
     4621 ggactcgagc gaccgagcgg gtgcctccag gtcggcgcgc tcgatgatct cctctgcacc
     4681 gagaagatgc aggtagggag cgctctcatg ccgtccactt gaggccacga cccggtgccc
     4741 ggcaccggcc agcagcaaga tcgcgatgct cccgacgcct ccgctggccc ccgtcactag
     4801 gactccggga cggtcagtcc actcttcctc ggtagccacg attcccgcgt cctccagcgc
     4861 tatgacggcc aacatcgcgg tcaatcccgc cgtgcccagc gccatcgcgt gcatcggaga
     4921 gaggccctcg ggaagcggta ccagcccctc ggaacggacg cgtgcaaact cggcgaagcc
     4981 gccccaatgg gattccccga tgccccagcc ggtgaggatg acttgctgtc cgatgcggta
     5041 gtcggcagcc gcagaccgca ccaccgtgcc cgccaagtcg atcccgggga ccatcgggta
     5101 ggaacggacg acgcgaccgg cgccggtaac ggccagggca tccttgtagt tcagggacga
     5161 gaaggccacc tcgacgacca cgtccccctc cggcaggtcg gagaactcga ccggctccac
     5221 cgctacctcg ggctcttcct ctcctccctc ccgcagcaca agtgctcgga aggagccgcg
     5281 ctcgctcatg agcatcatca cacctctccg tcgaaggggt tcgtccttgc ggcgagcgag
     5341 aagagatacg gatactcgtc tctcaggtgg ccgacgtacg ccaaccagga aggcaccagc
     5401 aggccgtaga cgcggctcgc gtcaccactc aaatgagctc gatccttctg cgagatgccc
     5461 gagagatcgg catgcaccgc caactcctcc gtgagatgga aggtggcgcg gagcaggtcg
     5521 gtgaaggtct cgtgctcctg gagggtagga ttctcgagga tgcggaccag caggggcctc
     5581 ttctccagga gagactccca gagagcggga aggtcgaggt cttcgagcgc gacccggtag
     5641 cgcccgcccc gcactcggct cgccgcctcg acgaactgtc ggtgctccca cgtgcccaag
     5701 cccgccagcg acgcggtgag ttcgggcagg tg
//
LOCUS       DASRMJ010000132         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_299768, whole genome shotgun sequence.
ACCESSION   DASRMJ010000132 DASRMJ010000000
VERSION     DASRMJ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299768"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1025
                     /locus_tag="VFD74_03785"
     CDS             66..1025
                     /locus_tag="VFD74_03785"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF002142.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HZK48705.1"
                     /translation="MFVFEKEQKVFDIGGVRVGGQPGENSPLIIPSLFQKGDPCVESR
                     RERTFNKQMAGDQIKRLEELSELTGIPAIVAMVANSGDEMKAYIDFFVSKTSMPFAID
                     MWNLEPRLAATAYVAELGLMDRLLYNSVTVWSPDVAAEVEQIKALGVKHVVTVVMDMV
                     DQLPSGRMSALGDMMAKLEAGTFESIIVDTSVMNLPSTGICALSNYKVKETYGIPAGA
                     ASANGTYMWQKSTWKDNKDAWGETAFMGLDAGTHAATIALWGDFIFSGPMWGNDHVFP
                     AVAAAHAMVSTMRYAETGRLTENESTPVYKLFPDFIKTLKGQA"
     gene            1076..2107
                     /locus_tag="VFD74_03790"
     CDS             1076..2107
                     /locus_tag="VFD74_03790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004889.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtaA/CmuA family methyltransferase"
                     /protein_id="HZK48706.1"
                     /translation="MAMTSKERVLNLITGKPVDQVPASSGFGNVIVAGLEKYNLRFAH
                     VHLDAQEMADAAAATVELVGIESPIVPFDMGVLAEALGARLNTYPHSEDLLYPTLRDK
                     FVQTADDIVVPADMSSAGRVPLVCEAIQILKKRFGDDYPVGAWVLGPFTLAGQVWDLN
                     DLLKSSAKDPAKVEAILSKLTDALMLEAKAYADAGADFITLREMGATSDVLSPRMFKK
                     TVKSWSEKILASISLPKVYHICGGTDMIVTDMAEVGADAISVDQKNDVAATREKLGPD
                     ATIFGNVHPYDVFTAGTPEMVREAVQKAIDAGVDAVWPGCDIWPDTGTENLKIWVDMV
                     HGMKPRRKA"
     gene            2237..2899
                     /locus_tag="VFD74_03795"
     CDS             2237..2899
                     /locus_tag="VFD74_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821221.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK48707.1"
                     /translation="MSEERLAELLKELHVAVVEYEEDRVAVAAQAVVDEGFDAYVAIM
                     DGLAAGMEEVGVLFDTQEYFVPEVLMCADALYAGLDILRPHVDMTGRESVRGEVVIGV
                     VEGDVHDIGKNLVKMMFEVAGFTVHDLGADVPLDQFIEKQISTNSEIVALSAMMTTTM
                     MGMKKAIVKLKEKNPNVMIMVGGAPVTGEIADMFGADGYSENASNAVSEAIKMVGALR
                     TM"
     gene            3060..>3516
                     /locus_tag="VFD74_03800"
     CDS             3060..>3516
                     /locus_tag="VFD74_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK48708.1"
                     /translation="MTFAPRRGEEQSMEHYHISEQPYYRPAGDEVELFERAHRLKLPV
                     MLKGPTGSGKTRFLQHMAFRLGLPLVTVACHEDLTASDLVGRYLFVGNETQWVDGPLT
                     LAVKYGAICYLDELVEARKDTTVVIHPLGDDRRILPIEKKGSVVEAHEDF"
BASE COUNT          698 a         1078 c         1143 g          597 t
ORIGIN      
        1 gccttaagga agtcctgacc gaagaaggcc tcggcgacct gatcagcagc taggaggtgg
       61 cgcgcatgtt cgtgttcgag aaggaacaga aggtcttcga cataggtgga gtgagagtcg
      121 gcgggcaacc gggcgagaac tccccgctca tcatcccttc gctcttccag aagggcgatc
      181 cctgcgtgga gagccgtcgg gagcgtacgt tcaacaagca gatggccggc gatcagatca
      241 agcggctcga ggagctgtcc gaactcaccg ggatccccgc catcgtggcg atggtggcca
      301 actccggcga cgagatgaag gcgtatatcg acttcttcgt ctccaagacc tcgatgcctt
      361 tcgccatcga catgtggaac ctggagccgc gtcttgccgc taccgcctac gtcgccgagt
      421 tgggtctcat ggatcggctc ctctacaact cggtcacggt gtggagtccc gacgtcgcgg
      481 cggaggtcga gcagatcaag gcgctgggcg tcaagcatgt cgtgaccgtg gtcatggaca
      541 tggtcgacca actgcccagc ggccgcatgt ccgcgctggg cgacatgatg gccaagttgg
      601 aggccggcac gttcgagagc atcatcgtgg acacctcggt gatgaacctc ccctccacag
      661 gcatatgtgc cctctcgaac tacaaggtca aagagacgta tgggatcccg gccggcgccg
      721 catctgcgaa cggcacctac atgtggcaga aatccacctg gaaggacaac aaggacgcgt
      781 ggggagagac cgccttcatg gggctcgacg cgggaaccca cgccgccacg attgcgctct
      841 ggggagactt catcttcagc ggaccgatgt ggggcaacga ccacgtgttt cccgcggttg
      901 cagccgccca tgcgatggtc agcaccatgc gctacgctga gacgggacga ctcaccgaga
      961 acgagagcac gccggtatac aaactgttcc ccgatttcat caagaccctg aagggtcagg
     1021 cctgacaggc tccacaccgg agccatacca agactatgag gaaggagcga ccagcatggc
     1081 gatgacctcc aaggagcgag tgctgaatct gatcacgggg aagccggtgg atcaggtgcc
     1141 ggcatcctcg ggattcggca acgtgatcgt tgccggtctc gagaagtaca acctgcgttt
     1201 cgcgcatgtt cacctggatg cgcaggagat ggccgacgcg gccgccgcca ccgtcgagct
     1261 cgtggggatc gagtccccca tcgtgccgtt cgacatgggc gttctcgctg aggcgctcgg
     1321 tgctcggctg aacacctacc cccactcaga ggatctgctc taccccaccc tgcgcgacaa
     1381 gttcgtgcaa acggctgacg acatcgtggt gcccgcggac atgtccagcg ctggccgcgt
     1441 cccgttggtg tgtgaggcca tccagatcct caagaagcgg ttcggcgacg actatccggt
     1501 gggcgcatgg gtgctcggcc ccttcaccct ggccggccag gtgtgggatc tgaacgatct
     1561 gctgaagtcg agcgccaagg atccggccaa agtggaggcc attctctcca aactgaccga
     1621 cgccctgatg ctcgaggcga aggcgtatgc ggacgctggc gccgacttca tcacgctgcg
     1681 cgagatgggg gccacctctg acgtgctctc tccccgtatg ttcaagaaga cggtgaagtc
     1741 gtggtctgag aagattctgg ccagcatcag tctgccgaag gtgtaccaca tctgcggtgg
     1801 caccgacatg atcgtcaccg acatggccga agtgggtgcc gacgcgatct ccgttgacca
     1861 gaagaacgac gtggctgcga cgcgcgagaa gctcggcccc gatgccacca tcttcggcaa
     1921 cgtgcaccca tacgatgtct tcaccgccgg cacgccggag atggtgaggg aggccgtgca
     1981 gaaggccatc gacgcaggcg tggacgcggt gtggcccgga tgcgacatct ggcccgacac
     2041 cggcaccgag aatctgaaga tctgggtgga catggtccat ggtatgaagc cccgccggaa
     2101 ggcgtaggag tcacaggact cattgggcgg ccgccggcgc gagccggcaa ccgcccgccc
     2161 ccggccggag gggccgggtg tcgttgggag ggtcggcggg acctgcgggg accgcccaca
     2221 gtcgaaggag ggacacatgt ccgaagaacg tttggcagag cttctcaaag agctgcacgt
     2281 ggctgtagtc gagtacgaag aggaccgcgt cgccgtcgcc gcgcaggccg tggtcgacga
     2341 aggctttgac gcctacgtgg caatcatgga cggcctggct gccggcatgg aagaagtggg
     2401 tgtgctcttc gacacccagg agtacttcgt tcccgaggtg ctcatgtgcg ccgacgcgct
     2461 gtacgccggt ctcgatatct tgaggcccca cgttgacatg acaggccgcg aatctgtcag
     2521 gggcgaagtg gtcatcggcg tggtcgaggg tgacgtgcac gacatcggca agaacctcgt
     2581 gaagatgatg ttcgaggtgg ccggtttcac cgtccacgac ctgggcgccg acgtgccgct
     2641 tgatcagttc atcgagaagc agatctccac caattctgag atcgtggccc tctccgccat
     2701 gatgaccacg accatgatgg gaatgaagaa ggcgatcgtg aagctgaagg agaagaaccc
     2761 gaacgtgatg atcatggtag gcggagcccc cgtcaccggc gagatcgcag acatgttcgg
     2821 cgccgacggc tactccgaga acgcctcgaa cgcggtgtcc gaagccatca agatggtcgg
     2881 cgctctccgc accatgtagt aggcgaccgg caacatccgc tcctcacttc cgcggtccgc
     2941 gcctctccgg ccgggccgcg cggaggaggg gcggcgcctt ctcgggcccc gggaactcga
     3001 tcttcggacg acttcggcgc agggcccgtc atacatcaga ccgacccggg gggggcggta
     3061 tgacgttcgc gcctcgtcgc ggcgaagagc aatcgatgga gcactaccac atctccgagc
     3121 agccgtacta tcgaccggct ggggacgaag tggagctgtt cgagcgcgca cacaggctca
     3181 agctaccggt catgctgaag gggcccaccg gcagcggcaa gacacgcttc ctccagcaca
     3241 tggctttccg cttggggcta ccgcttgtga cagtggcctg tcacgaagac ctgaccgcga
     3301 gcgacctggt gggccgctac ctcttcgtgg gcaacgagac ccagtgggtc gacggaccgc
     3361 tcaccctcgc ggtcaagtat ggcgccatct gttatctgga cgaactggtg gaggcccgca
     3421 aggacaccac ggtggtcatt catcccctcg gcgacgaccg ccgcatcctc cccatcgaga
     3481 agaagggcag cgtcgtcgag gcacacgagg acttca
//
LOCUS       DASRMJ010000133         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_451329, whole genome shotgun sequence.
ACCESSION   DASRMJ010000133 DASRMJ010000000
VERSION     DASRMJ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451329"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VFD74_03805"
     CDS             complement(<1..919)
                     /locus_tag="VFD74_03805"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZK48709.1"
                     /translation="MSTAAGEGPFVAGQHSFVVTETEEGQRLDVYLGSMPSIGSRSQA
                     SLLFEQGSVEVNGRVRPKSFRLTSGDSVRVVFSDVPSPPAQASSRSVPVVYEDEWLLV
                     ADKPADMVVHPAKGHAADTLVDALRGHGLAGGEEFRPGIVHRLDKDTSGLLVVAKDVA
                     VHRALQQSIRQRLVDRRYLALVHGDISTPTGTIEAPIGRDRQRRKNMAVGGAAARDAV
                     THFTVVERFGLFTLVEARLETGRTHQIRVHFLAIGHPVVGDPTYARRDALGLGRQFLH
                     SHRLRLRHPVGGAELDLSSPLPADLAGVLD"
     gene            complement(916..1401)
                     /locus_tag="VFD74_03810"
     CDS             complement(916..1401)
                     /locus_tag="VFD74_03810"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZK48710.1"
                     /translation="MPVVGRLRWEVVAGLAAAVLVLDLGTKRAIRGTLALGESRDLLP
                     FLELRHHANDGAAFGLLSGRQSFILVAVGLAMAAVVAYVFLDRRLVAAVAGGLLVGGS
                     LGNLVERVAYGRVTDFLQLPHYPTFNVADIFIVAGVGLMAITLVRDYLRGPSEGGSAD
                     A"
     gene            complement(1445..1804)
                     /locus_tag="VFD74_03815"
     CDS             complement(1445..1804)
                     /locus_tag="VFD74_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZK48711.1"
                     /translation="MDREQVREVLTEERERLVREIESLEGDPMESPTEVPEEIHPRLH
                     MAENAAYTLDRQMELTLGENARHVLARIERALAKLESGSYGLCDSCGASIEAARLEAV
                     PYAGFCIDCQRREERGA"
     gene            complement(1818..2135)
                     /locus_tag="VFD74_03820"
     CDS             complement(1818..2135)
                     /locus_tag="VFD74_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZK48712.1"
                     /translation="MEPIELRCTDGEVLVGFRVSPSARRTRVQGRYGDRIKVQVSAPP
                     EDDRANSELVGAVAGWLGLPTGCVSIRSGHKSRDKLLSFKDIDEPSLRTLLEELLGRA
                     STG"
     gene            complement(2193..>2616)
                     /locus_tag="VFD74_03825"
     CDS             complement(2193..>2616)
                     /locus_tag="VFD74_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48713.1"
                     /translation="VEAPATEAPAAEAAAEPSAADAAVALPSGVGSAPAEAPVSGEAM
                     SGVSQWPSDDLGSAAVIETPAGADVATDSEQVAPEDAEEPETVADELEDAGGRAEDED
                     STDARKDSSVRRFLFGRKDKQSGEGDFFDGPEDRDFQW"
BASE COUNT          453 a          952 c          789 g          422 t
ORIGIN      
        1 agtcgagcac tccggccaga tccgcgggca gcggagaact caggtccagt tcggcaccgc
       61 ccaccggatg gcgtagccgc agcctgtggc tgtgcaggaa ctgcctgccc aagcccaacg
      121 cgtcgcgtcg cgcataggtg gggtcgccca cgaccgggtg tccgatagcg aggaagtgca
      181 ccctgatctg gtgggtccgc cccgtctcca accgggcctc cacaagtgtg aacagcccaa
      241 acctctcgac caccgtgaag tgggtcacgg cgtcgcgagc ggcggcacca cccaccgcca
      301 tgttcttccg ccgctgacga tcgcggccga tgggcgcctc gatcgtaccg gtgggcgtgg
      361 agatatcgcc gtgcaccagg gcaaggtagc gccgatcgac gagccgttgc ctgatcgact
      421 gctgcaacgc acgatggaca gccacgtctt tagccactac cagaagaccg ctcgtgtcct
      481 tgtcgagtcg gtgcacaata cccggccgga actcctcacc tcccgccaga ccgtgccctc
      541 ggagcgcatc gaccaacgta tcggcagcat gccctttggc cgggtggacg accatatcgg
      601 ccggcttatc cgcgacaaga agccactcat cttcgtacac gaccggtacg gaccgggagg
      661 acgcctgcgc gggcggcgaa ggcacgtcgg agaagaccac gcggacggaa tccccgctgg
      721 tcaatcgaaa gctcttcggg cggacgcgcc cgttcacctc tacggatccc tgctcgaaca
      781 gcagcgacgc ttgtgagcga cttccgatgg acggcatgga cccgaggtag acgtcgagcc
      841 gctgcccctc ctccgtttcg gtgacgacga aggagtgctg tccggcgacg aaagggcctt
      901 ctccagccgc ggtgctcatg cgtccgccga gccgccctca gacggacctc gaaggtagtc
      961 ccgcacgagg gttatcgcca tcaagccgac cccggccacg atgaagatgt cggcgacgtt
     1021 gaacgtaggg tagtgcggca gttggaggaa atcggtcacc cgcccgtagg ccaccctttc
     1081 gaccaggttg cccaagctcc cccccacgag cagccctccc gccacggcgg ccaccaggcg
     1141 ccgatcgagg aacacgtacg ccacgaccgc cgccatggcc aggcccacgg ccacgaggat
     1201 gaacgactgt cttcccgaca agagtccgaa ggcagcccca tcgttggcgt ggtggcgcaa
     1261 ctccaggaag ggcagaagat cgcggctctc tccgagcgcg agcgtccctc ggatagcccg
     1321 cttcgtgccc aagtcgagaa ccaacaccgc ggcggcgagc ccggcgacca cttcccagcg
     1381 caaccttccg actacaggca cttttatgcc ttccccaggg gtaaccgcat acaaagaccc
     1441 gtcactacgc cccccgctct tccctgcgct ggcaatcgat gcagaacccc gcgtacggaa
     1501 ctgcctccaa cctcgcggcc tcgatgctcg ctccgcacga atcgcagagt ccgtacgaac
     1561 cgctctccag cttcgccagt gcgcgctcga tgcgcgcgag cacgtgccgc gcattctccc
     1621 ccagggtcaa ctccatctgc cgatcgaggg tgtacgcggc gttctccgcc atgtgcaatc
     1681 gggggtggat ctcctcgggc acttccgtcg gtgactccat cggatctccc tcgagggact
     1741 cgatctcacg cacgaggcgc tcgcgctcct cggtgagtac ttcgcgcact tgttcgcggt
     1801 ccatggttgc cacactccta tcccgtagac gcccggccga gcaactcttc gagaagcgtg
     1861 cgtagactgg gttcgtcgat gtccttgaat gagagcagct tgtcccgact cttgtgcccg
     1921 gagcggatac tgacgcaacc ggtcggtaga ccgagccatc cggccacggc tcccaccagc
     1981 tcggagtttg cccgatcgtc ctcgggaggc gcgctcacct gcaccttgat ccgatcgcca
     2041 tatcgaccct ggacccgggt ccgccgagcg gagggactca cccggaaccc caccagcacc
     2101 tcgccgtccg tacaccgcag ctctatgggt tccacaccga ccgcctcccg cgcccgccca
     2161 tgcgggctcc tacgggcgcg attcccggaa tctcaccact ggaagtcgcg atcctccgga
     2221 ccgtcgaaga aatcgccctc tccagactgc ttgtctttgc gcccgaacag gaatcgccgc
     2281 accgatgaat ccttgcgcgc gtcggtggag tcctcgtcct ccgcccgccc gccagcgtcc
     2341 tcgagttcgt ccgcgacggt ctcgggctcc tccgcgtcct ccggtgccac ctgctcggaa
     2401 tccgtcgcga cgtcggcgcc ggcgggggtc tcgatgaccg cagctgaacc gaggtcatca
     2461 ctcggccatt gggagacgcc cgacatggct tcgcccgaga cgggcgcttc ggcaggcgcc
     2521 gatcccacgc ctgacggcaa ggcgacggcg gcatcagcgg ctgacggctc ggcggcggct
     2581 tcagcggcgg gcgcttcggt ggcgggcgct tcgacg
//
LOCUS       DASRMJ010000134         5615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_451382, whole genome shotgun sequence.
ACCESSION   DASRMJ010000134 DASRMJ010000000
VERSION     DASRMJ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451382"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFD74_03830"
     CDS             <1..753
                     /locus_tag="VFD74_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZK48714.1"
                     /translation="EDTTSYLRGLVTGEAWTYGDNWYRRDFIGPVLPRFVRSLRYLMT
                     SAVLATLLGLLAGAWLTAARRDRLKDVVTFAGAVPDFVLALLLQLAVVAVYRATGFRV
                     ARVASLTNRDAAVLLPLISLTLVPAVYLARSLSARAYVIASEDFVLVSKAMGQGRPRI
                     WLAQILPNLTAFLRADLAKVCGMMLANFFIVDYLYNLKGVTTMLFMFGFTHPYQYNLT
                     MDTFIVMIVLYVIIYVGLRGLVRVIDGVAVRL"
     gene            888..1781
                     /locus_tag="VFD74_03835"
     CDS             888..1781
                     /locus_tag="VFD74_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZK48715.1"
                     /translation="MIGLVMVAVLLVAAVAGPALAPYPRDYSEKIYVQQTTEGTVLLY
                     APQPPSLRHLFGTDRWGFDILSLLLFGAKYTVFGALGVALARVAFGTGLGLFSSIGRG
                     GTTRPTRVAGILGSIPAFVIVYFVMVGINVNSPLGPVTLTLIQGTVMTLIGLPSVAAV
                     VREKSDRLKRSDHVTAAVALGAGRRRVAARHILPLLREDIMILSVHEVVFVLTLLGQL
                     AIFDMFLGGTVRWEDPPTYVPLTHEWAGLIGYARPAVQVYQWILGAPLAAFLFAVFAF
                     QILTTGMERRMRVEYLKTPHA"
     gene            1915..3333
                     /locus_tag="VFD74_03840"
     CDS             1915..3333
                     /locus_tag="VFD74_03840"
                     /inference="COORDINATES: protein motif:HMM:NF018498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HZK48716.1"
                     /translation="MAEKTVLLVTNLDTRGAEFAQVRDLVLAQGLDALIMDVSMETAP
                     PFPGDITCEHVAEAGGLTIEAVREAYHAERKVSTDCMIRGGGELARCLYDEGRIHGVL
                     GAGGATATLIATSIMKRLPFGLPKVMASSVGSHPGYVGRYVGTKDIMMLNTVVDVMGT
                     NHLLWQQLRNAAGAVCGAVKVYERPPGEGAAAPRAGATEPAVAGPAVVDPTAAVVTTT
                     LAAGSAARPIVGITSFGFAERCVERLIEVLRAAGFEPVPFHAQGQGDRAMDELIRDGM
                     LAGVIDVVTRGVVEELLGGNCAAGLDRVLAAAEMGVPQVIAPSGLDMLSVGGQADWRT
                     RFSGRPYAVIDELRVEVRTSAEECRAAARIVAERLNRATAPFRFLVPTLGWSSLDREG
                     APLWDPAADAAFVAELRSCLDDPTLVVEVAANLYSTTFADACAEAFLEVWGGAGATEN
                     TGSRRRCAIGRRAGPRRAPNRA"
     gene            complement(3320..4234)
                     /locus_tag="VFD74_03845"
     CDS             complement(3320..4234)
                     /locus_tag="VFD74_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZK48717.1"
                     /translation="MFLNLNQLRVFYTAAREGSYRAAAQVLFITQPAVSQNIKALEVY
                     YGVGLFQRVGRSMQLTQAGRILFEHAERIFAAADEAEHAIRELGKLERGEIRVGTTKI
                     YARYLMPSIVNAFREQHPAITVALDEGSSEQMVNSVENLKNHLVVVGRVPYPPRLRTQ
                     HFRLVELTLVAGPGHPLSGLAPAHWKDLDGQPLIVRESGSGTRRAVTERLAQERVSPR
                     VVMESSSVDFIKRYVAEGRAVAFLFEPDMREELTAGRLVRVPLAEGELTLETDVVVLA
                     DAYKSPAVRAFMRVLEDSGTAEWSVMPD"
     gene            4413..>5615
                     /locus_tag="VFD74_03850"
     CDS             4413..>5615
                     /locus_tag="VFD74_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK48718.1"
                     /translation="MTTYDGIILGGGPNGLTLAAYMARAGLKVLVLERRMEIGGGLAT
                     EKVTIPGWIHNTHAVYHMMVDYAPVFQDLELFSRHKLEFVKPELSMTMPLSDGRAVCL
                     YDDPKKTAENFAHFSKADASAYLEMNAHFTNLVDNFIAPATYAEAHPAMEQLENLQKT
                     EIGREIQAIQSKSPLEIVESWFENEHIKGLMLYAACFWGLEHDLEGVGFLVPLMIERA
                     AKYRLLKGGTHQLSNRLSKDIYAHGGMILGSQLVKRIIIDDGRATGVELEDGTVHLAT
                     KFVASSLNPHQTFLDYVGEQHLDADFVTRIKDWQWDTWSLNGVHLALAEPPDFISAKF
                     NPDVNKAFVHILGYDSHEDMIDHWDAIHDGELLPEAGFNACFPSVHDPYQAPAGKASG
                     LLSEHAPYD"
BASE COUNT          919 a         1908 c         1820 g          968 t
ORIGIN      
        1 gaagacacga cctcctacct gcgcggcctt gtgaccggcg aggcctggac ctacggcgac
       61 aactggtacc gccgcgactt catcgggccg gtgctgccgc ggttcgtccg ctcgctcagg
      121 tacctgatga cctcggccgt cctggccaca ctgctcggcc tgctcgcagg cgcgtggctg
      181 accgccgccc ggcgagacag actcaaagac gtggtgacct tcgcgggggc ggttccagac
      241 ttcgtgctgg ccctgctgct tcagttggcg gtcgtggccg tgtatcgagc caccgggttc
      301 cgcgtggccc gcgtcgccag cctcacgaat cgcgacgccg ccgtccttct gccgctcatc
      361 tcgctcactt tggtgcccgc cgtctacctg gcccgctcgc tctcagcccg agcgtatgtg
      421 atcgcctccg aggacttcgt cttggtgtcc aaggccatgg gacaaggcag accgcgcatc
      481 tggctcgcgc agatccttcc gaacctcacc gcgttcctgc gtgccgacct ggcgaaggtc
      541 tgcgggatga tgctcgccaa cttcttcatc gtggactacc tctacaacct gaagggtgtc
      601 accacgatgc tcttcatgtt cggcttcacg cacccctacc agtacaacct caccatggac
      661 accttcatcg tgatgatcgt gctgtacgtg atcatctacg tcggcctgcg cggacttgtg
      721 cgcgtgatcg acggcgtggc ggtgcgtcta tgagagccgg agaaggcgcc tcgccccgac
      781 ggtccgatcc gcggtccgat cggcggcccg atccgcggcc cggcgacccc cgggccacgc
      841 ggcagacgtc gcggcggagg cgctccggac gcggcacacc ggagatcgtg atcgggctgg
      901 tcatggtcgc cgtgttgctg gtggcggcgg tcgcggggcc ggctctggct ccctatccgc
      961 gcgactactc cgagaagatc tacgtgcagc agaccacgga gggcaccgtg ttgctctacg
     1021 ctccgcagcc tccctctctg cgccatctgt tcggcaccga ccgctggggc ttcgacatct
     1081 tgtcgctgct cctgttcgga gcgaagtaca cggtcttcgg agcgctcggg gtggctctgg
     1141 cccgagtggc gttcgggacc ggattggggc tgttctcgag catcggacgg gggggaacca
     1201 cgcggccgac cagggtggct gggatcctgg gcagcatccc ggctttcgtg atcgtctact
     1261 tcgtcatggt gggcatcaac gtgaactcgc ccctgggccc cgtcacgctg accctcatcc
     1321 aagggacggt gatgaccctc atcgggcttc cgtcggtggc ggcggtggtg cgggagaagt
     1381 ccgaccgcct gaagcgcagc gaccacgtca ccgcggccgt cgctctggga gcggggaggc
     1441 gccgcgtcgc cgcgaggcac atactccccc ttcttcgcga ggacatcatg atcctgtccg
     1501 tgcacgaggt ggtgttcgtc ctcacccttc tcgggcaact ggccatcttc gacatgtttc
     1561 tcgggggaac ggtgcggtgg gaggatccgc ccacctacgt gcccctgacc cacgagtggg
     1621 ccggcctcat cggctacgcg cgcccggccg tgcaggtgta ccagtggata ctgggagcgc
     1681 cgctggcggc cttcctgttc gcggtcttcg cgttccagat cctgacgacc gggatggagc
     1741 ggcgcatgcg cgtggagtac ctaaagacgc ctcacgcgta ggcggagtgc caccggcggc
     1801 ctcgcgggct tgagcgcgta ggcagcccca cccccggttg cgcggccggc cagccgatgc
     1861 gatagtggca gccccgcagc acacggggat tcacgccgac atgagggagc gggtgtggcc
     1921 gagaagaccg tcttgctggt gaccaactta gacaccaggg gagccgagtt cgcacaggtg
     1981 cgcgacctgg tgttggcgca ggggttggac gccttgatca tggacgtctc gatggagacc
     2041 gcacccccct tccccggcga catcacctgc gaacacgtgg ctgaggcagg cggcctcacg
     2101 atcgaggcgg tccgtgaggc gtaccacgcc gagcgcaagg tctcgaccga ctgcatgatc
     2161 cggggcggcg gcgaattggc ccggtgcttg tacgacgagg gccgcatcca cggcgtgctc
     2221 ggcgcgggcg gcgccaccgc caccctgatc gccaccagca tcatgaaacg gctgccgttc
     2281 gggctgccca aggtcatggc ctcctccgtg ggcagccatc ccggctacgt ggggcggtac
     2341 gtcggcacca aggacatcat gatgctgaac accgtggtcg acgtcatggg caccaaccac
     2401 cttctctggc agcagttgcg caacgccgcc ggcgccgtct gcggcgcggt gaaggtgtac
     2461 gagcggccgc ctggtgaggg cgccgccgcc cctcgcgcgg gggcgaccga gccagcggtg
     2521 gccggcccgg cggtggtcga cccaacggcg gcagtcgtca ccacgacact cgccgccggc
     2581 agcgccgcac gccccatcgt cggcatcacc tctttcggct tcgccgagcg gtgcgtggag
     2641 cggctcatcg aggtgctgcg ggccgccggc ttcgagcccg tccctttcca cgcccaaggg
     2701 cagggcgacc gcgcgatgga cgagttgatc cgcgacggca tgctggccgg tgtcatcgac
     2761 gtggtcacgc gtggcgtcgt ggaggagctt ctgggcggca actgcgcggc cggcctcgac
     2821 cgtgtcctgg ccgccgccga gatgggcgtg ccgcaggtga tcgcgccgag cggcctcgac
     2881 atgctctcag tgggaggaca ggctgactgg cgcacccggt tctccggacg gccgtacgcc
     2941 gtgatcgacg aactccgcgt ggaagtgcgc acatcggcgg aggagtgccg ggcggccgcc
     3001 cggatcgtgg ccgagcgtct gaaccgcgct acggcgcctt tccgcttctt ggtgccgacg
     3061 ctcggctggt cgtccctaga ccgtgaggga gcgcccttgt gggacccagc ggccgacgcc
     3121 gcgttcgtcg ccgagctccg ctcgtgcctg gacgacccga cgctggtggt cgaagtcgcc
     3181 gccaacctct attcgaccac gttcgccgat gcctgcgctg aagcgttcct ggaggtgtgg
     3241 ggcggggcgg gcgcgactga gaacaccggc tcgcgccgcc gatgcgccat cggccggcga
     3301 gctgggccgc gccgcgcccc taatcgggca tgacgctcca ctcggccgtg ccggagtctt
     3361 ccaacacccg catgaaggcc cgcaccgccg gcgacttgta ggcgtcggcc aagaccacga
     3421 cgtcggtctc gagcgtaagc tccccctcgg ccagaggcac acggaccaag cggcccgcgg
     3481 tgagttcctc ccgcatgtcc ggctcgaata ggaaggccac cgcccggcct tccgccacgt
     3541 accgcttgat gaagtccacc gagctcgact ccatgaccac gcgcggcgac actctctctt
     3601 gcgccaaccg ctccgtgacg gcccgccgag tgccggagcc actctcgcgg actatcagag
     3661 gttggccatc gaggtccttc cagtgggcag gcgccaggcc gctgagcgga tgcccgggcc
     3721 ccgccaccaa cgttagctcc accaggcgga agtgctgggt gcgaagccga ggcgggtagg
     3781 gcacccgtcc aacaacaact agatgattct tgagattctc tacgctgttc accatctgct
     3841 cggagctgcc ctcgtccaac gctaccgtga tggccgggtg ctgctcgcgg aaggcgttca
     3901 cgatggaggg catgaggtag cgggcgtaga tcttcgtggt cccgactcgg atctcaccgc
     3961 gctccagctt gcccagctcg cgtatcgcgt gctcggcttc atccgcagct gcgaagatgc
     4021 gctcggcgtg ttcgaagagg atacgtccgg cctgcgtgag ttgcatcgag cggccgaccc
     4081 tctggaagag gcccactccg tagtacacct cgagcgcctt gatgttctgg ctcaccgcgg
     4141 gctgggtgat aaagaggacc tgcgcagccg cgcggtagct gccctcccgg gcggcggtgt
     4201 agaagacgcg aagctggttg aggttcaaga acacggtctc tccagcgagg cattcattcc
     4261 agatccggca tacataagca tagctgattg ttccatcagc gcaactacct tgtgccgttc
     4321 gtcatcaggc gtagtatcgg actcgctcga ggcataccgc acaatcgggg cctccgagct
     4381 gcgggatcga tcaggacgtc tacgggggga ccatgacgac ctacgatgga atcatccttg
     4441 gcggcgggcc gaacggcctc actctggccg cctacatggc tcgagcggga ctcaaggtcc
     4501 tggttctcga gcgacgcatg gagatcggag gcggcctggc caccgagaag gtgaccattc
     4561 ccgggtggat ccacaacacc cacgccgtct accacatgat ggtcgactac gctcctgtct
     4621 tccaagatct ggaactcttc agcagacaca agctcgagtt cgtcaagccc gaactcagca
     4681 tgaccatgcc actctccgac gggcgtgcgg tctgtctcta cgacgacccc aagaagacgg
     4741 ccgagaactt cgcccatttc tcgaaggccg acgccagcgc ctacctcgag atgaacgcgc
     4801 acttcaccaa cttggtcgac aacttcatag cacccgccac ctatgcggaa gcccacccgg
     4861 ccatggagca actcgagaat ctgcagaaga ccgagatcgg gcgcgagatc caggcgatcc
     4921 aatccaagag tccgctggag atcgtcgaga gttggttcga gaacgaacac atcaagggcc
     4981 tcatgctcta cgccgcttgc ttctggggac tcgagcatga cctcgaagga gtgggcttcc
     5041 tcgtcccgct gatgatagag cgcgcggcca agtaccgcct gctcaaaggg ggcacacacc
     5101 agctgtccaa ccgcctctcg aaagacatct acgcccacgg gggcatgatc cttggcagcc
     5161 agctcgtcaa gcgcatcatc atcgacgacg gacgggccac gggtgtggag ctggaagacg
     5221 gcaccgtcca cttggccacc aagttcgtgg cttcgagcct gaatccgcac cagaccttct
     5281 tggattatgt cggcgagcag cacctggacg ccgacttcgt cacccgcatc aaagactggc
     5341 agtgggacac ctggagcctg aacggcgtgc acctggcact ggccgagccg ccggacttca
     5401 tctcagccaa gttcaatccc gacgtgaaca aggctttcgt ccatatcctg ggctacgact
     5461 cccacgagga catgatcgac cactgggacg ctatccacga cggggagttg ctgcccgagg
     5521 ccggcttcaa cgcctgcttc ccgtcggtgc acgatcccta tcaggcgccg gcgggcaagg
     5581 ccagcggcct gctgtccgag cacgcgccgt atgat
//
LOCUS       DASRMJ010000135         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_191394, whole genome shotgun sequence.
ACCESSION   DASRMJ010000135 DASRMJ010000000
VERSION     DASRMJ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191394"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="VFD74_03855"
     CDS             complement(<1..1717)
                     /locus_tag="VFD74_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZK48719.1"
                     /translation="MSLGAADSVGAADSVGAAHSAGTPDVAGARPGRDIVEAALAAGH
                     AALDESSGKALFVAYGIPVTPGQVVGTEEVAVQIAMELGYPVVMKGMSREILHKTDAG
                     LVILDVDDEEAVRSGFHVLQRRGEGRLDGVLVEKMLPRGREFVAGLTRDPQFGPVIMF
                     GVGGILTEALDDVAFGVAPLDEREANELLGAIRASRLLGEFRGSPAVDRPALVHIICA
                     LGRMAFDHPEIQEIDVNPVLVDGSRPVAVDALVTLGPPVAPPTKEFTAALGNLDAVFT
                     PKAVAVVGASNDPTKWGGTIMNNIISGGYAGGLYPVNPRSDQIFGLKAYTSLSELPEV
                     PDLVLIAVPAHLVPGVVEEAGSLGVRALVVITSGFSEVSAEGAALERQVAETAARHGM
                     AMIGPNCMGVISSWSHFYATGAIIIHPETGPASFLSQSGNMGVQLMAAAEGRKGGIGK
                     FVGIGNEALFDATDLFEYFRTDPQTGVILAYLEGFNDGRRFMEEARKATAQKPVIVLR
                     AAVSDYGLKAALSHTGTMAGSARVYEAVVRQSGIISTNDPDEFLDLAFSLSYMPLPKG
                     RRVAVV"
     gene            complement(1861..>2712)
                     /locus_tag="VFD74_03860"
     CDS             complement(1861..>2712)
                     /locus_tag="VFD74_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZK48720.1"
                     /translation="VLYALVSLVCAGLNDVVFKRYAVKDRSRGAYIFGIGIVWAVLQV
                     ALAGVRGVGLEFGETTLLWGLAAGALLTASNILLLESLAHIDVSLGSTIYRLNTVGVV
                     VLSLVFLGEQMGWHRGLGVALGLTAVLLLYRRGGHGEATGRRFALFFTLAVAASSFRA
                     AYGIVSKAGLADGAALPSVLVIGAVCWIVGGAVYAFLRENRLRITPKKAAYSLVSGVL
                     VFLIVNSLLLAVEHGEASVVIPIANMSFVVALLLSIGLRMERLTWRTAVAVVVSVCSV
                     ILLSLVA"
BASE COUNT          521 a          957 c          842 g          392 t
ORIGIN      
        1 tgaccaccgc cacccgccga cctttgggca gtggcatgta cgagagagag aaagcgagat
       61 cgaggaactc atccgggtcg ttggtggaga tgatgccgct ctggcgcacc accgcctcgt
      121 agacccgggc cgagcccgcc atcgtgcccg tgtgcgacag ggcggctttc agaccgtagt
      181 cgctgacggc cgcacgcaac acgatcacgg gtttctgggc cgtagccttg cgggcttctt
      241 ccatgaagcg gcgaccgtcg ttgaaaccct ccaggtaggc caggatgacc ccggtctgag
      301 gatccgtgcg aaagtactcg aacaggtcgg tggcgtcgaa caaggcctcg ttcccgatac
      361 cgacgaactt gccgatgccg cccttgcggc cctcggccgc cgccatcagc tgcacgccca
      421 tgttccccga ctgcgacagg aagctcgcgg gaccggtctc cgggtggatg atgatggctc
      481 cggtggcata gaagtggctc cacgacgaga tcacgcccat gcagttgggg ccgatcatgg
      541 ccataccgtg acgcgccgcc gtctccgcca cttggcgctc gagcgccgcg ccctcggcgc
      601 tcacttcgga gaatccggag gtgatcacca ccagcgcacg cacaccgagg ctgcccgcct
      661 cctccaccac gcccgggacc agatgagccg gaaccgcgat cagcaccagg tcggggactt
      721 ccggaagttc gctgagcgac gtgtaggcct tgagcccgaa gatctggtcg gagcgggggt
      781 tcaccggata caggccaccc gcgtaccccc cggagatgat gttgttcatg atggtgccgc
      841 cccacttggt ggggtcgttc gacgcgccca cgaccgccac cgctttcggg gtgaatacgg
      901 cgtccaggtt gcccagcgcc gccgtgaact ccttcgtagg cggcgccacc ggcggcccaa
      961 gggtgaccaa cgcatcgaca gccacagggc ggctgccgtc caccagcacc gggttgacgt
     1021 cgatctcctg gatctccggg tggtcgaacg ccatgcggcc gagggcgcag atgatgtgca
     1081 cgagagccgg gcgatccacg gcgggagacc cgcggaactc gccgaggagt cgcgaggccc
     1141 ggatggcgcc gagcagttcg ttggcttcac gttcatcaag cggagccacg ccgaaggcca
     1201 cgtcgtcgag ggcctcggtc aggatgccgc caactccgaa catgatcacg gggccgaact
     1261 gcggatcgcg ggtgagaccc gccacgaact cacggccccg gggcagcatc ttctccacca
     1321 acaccccgtc aagacgaccc tcgccgcgcc gctggagcac gtggaaaccg gaccggaccg
     1381 cttcttcgtc gtccacatcg aggatcacga ggcccgcgtc cgtcttgtgc aggatctcgc
     1441 gcgacatgcc cttcatcacc accggatagc cgagttccat ggcgatctgg acggcgacct
     1501 cctcggtacc cacgacctgg cccggcgtga ccgggatccc gtacgcgacg aacagcgctt
     1561 tgccggagga ttcgtcgagt gcagcgtgtc ccgcggccag cgccgcctcc acgatgtcac
     1621 gaccgggccg cgcccccgcc acgtcaggcg tgccggccga atgcgctgct ccgaccgaat
     1681 ccgctgctcc gaccgaatcc gctgctccga gcgacatcct gcctccttga tctctccaat
     1741 ggttccagat gaacggtgcc cgtcataccg ggcgccgaga cgctccgact gggtgtcttc
     1801 ggacaatagc acagggccgc gcgacgttta cccgcacggc ggcggttccg cgccgccgac
     1861 tcaggcaacc agcgacaaca ggatcactga gcacaccgaa accacgaccg ccaccgcggt
     1921 gcgccacgtc aatctctcca tgcggagtcc tatcgacagc agcaaggcca cgacgaagct
     1981 catgttcgcg atgggtatga cgacgctcgc ctccccgtgc tctaccgcca gcagtaggga
     2041 attgacgatc aagaacacca ggacccccga aacgagcgag tatgccgcct tcttgggggt
     2101 gatccgcagg cggttctcgc gaaggaaggc gtagaccgcg ccgcccacga tccagcagac
     2161 cgccccgatg accagcacgg acggcagagc cgccccgtcc gcgaggcccg ccttggacac
     2221 gatcccatac gccgcgcgga acgaagaagc ggccacggcc agggtgaaga acagggcgaa
     2281 acgccgaccg gtcgcctctc cgtggccgcc acgccggtag agcagaagca ccgctgtgag
     2341 gcccaaagca acgcccaggc ccctgtgcca ccccatctgc tcccccagga acacgagcga
     2401 aagcacgacc acgccgaccg tgttgagcct gtagatggtc gagccaagac tcacatcgat
     2461 atgagcgaga ctctcgagaa gaaggatgtt ggaagccgtc agcagcgcgc cggcggccaa
     2521 cccccagagt agcgtcgtct ccccgaactc gaggcccacg ccccgcacgc cggcaagggc
     2581 gacctgaagc acggcccaga cgatcccgat gccgaagatg taagcgccgc gagatctatc
     2641 cttgaccgcg tagcgtttga acaccacgtc gttcaatccg gcgcacacga ggctgaccag
     2701 cgcgtagagt ac
//
LOCUS       DASRMJ010000136         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_126338, whole genome shotgun sequence.
ACCESSION   DASRMJ010000136 DASRMJ010000000
VERSION     DASRMJ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126338"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VFD74_03865"
     CDS             complement(<1..1261)
                     /locus_tag="VFD74_03865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013335398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZK48721.1"
                     /translation="MGHGNDGLFLDLAGLGEDELVLREQAHRIAVEVLRPAAWALDRM
                     TPTERIAPGSPFFTTMATLKSLGYHRLFLPAEAGGPEEPLSPLAQSVVLEELGWGSLG
                     LATAFLVDMLPVITMSRFGSPELQAELMVPWVEDETGAFHGCWAITEPDHGSDFIGQR
                     DGDARTYGRGQLVAEPGDGGWVLRGQKSAWVSSGPIATHAAVHAQAGRDGDLFHSLFA
                     VVPLDDPGVRRGAPADMLGVRDDPQGELFFDEVFVPERNVLVPPGPFYPVFGDQLLCL
                     TSSVISNVAVGVARAAFEEALHHARQRIQGGGPIARHKNIQLTLYAMFERVETARAYS
                     RAALAHTQANVPGSSPGALGASPRHARAAQVYAKRAAYEVAHDALQVFGAQGLSRDSL
                     IEKLFRDARSLLIEDGTLEVLALDAARD"
     gene            complement(1643..>2186)
                     /locus_tag="VFD74_03870"
     CDS             complement(1643..>2186)
                     /locus_tag="VFD74_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZK48722.1"
                     /translation="SFDMMNTVHGLTQAVWDIRRLVSWIRAQGATSIGLYGVSLGGYV
                     VSLLAGIEEGFDAVVAGVPVVDFPALFHEHSPVHIRARSIEHRIMGGVAEDVYRVVSP
                     MSFESKVPRDHRYVFAGYGDRLAMPEQARRLWEHWDKPRISWYSGNHLGYLWSRQAAA
                     FLGESLAASGLSCTRAADEG"
BASE COUNT          373 a          760 c          730 g          323 t
ORIGIN      
        1 ggtcgcgggc ggcgtcgagg gccagcacct cgagcgtccc gtcctcgatc agcaggctcc
       61 gggcgtcgcg gaagagcttc tcgatcagcg agtcgcggct caacccctgg gcgccgaata
      121 cctgcaaagc atcatgggcg acctcgtagg ccgcccgctt ggcgtacacc tgcgcggcgc
      181 gggcgtggcg tggcgacgcc ccgagggccc caggggacga cccggggaca ttcgcctgcg
      241 tgtgggccag tgcagcgcgg gagtaggcgc gtgccgtctc cacgcgctcg aacatcgcgt
      301 acagggtgag ctggatgttc ttgtggcgag cgatggggcc gccgccctgg atgcgctggc
      361 gggcatggtg gagcgcctcc tcaaaggcgg cacgggccac gccgacggcg acgttggaga
      421 tgaccgagct cgtcaggcac agcagctggt ccccgaagac cgggtagaag ggcccaggcg
      481 ggacgagcac attgcgttcc ggcacgaaga cctcgtcgaa gaacagctca ccctggggat
      541 cgtcccgcac gccgagcatg tcggcgggag cgcctcgccg caccccggga tcgtcgaggg
      601 ggacgacggc gaacaggctg tggaacaggt cgccgtcgcg gccggcctgg gcgtggacgg
      661 ccgcatgggt ggcaatcgga cccgagctca cccacgccga cttctggccc cgcagcaccc
      721 agccgccgtc acccggctcg gccaccagct ggccccggcc gtaggtccgg gcgtccccgt
      781 cgcgctgccc gatgaagtcg ctcccgtgat ccggctcggt gatggcccag cacccgtgga
      841 aggcacccgt ctcgtcctcg acccacggca ccatgagctc ggcctgcagc tccgggctcc
      901 cgaaccggga catggtgatc accgggagca tgtcgaccaa gaaggcagtc gccagcccga
      961 ggcttcccca gcccagctcc tcgaggacca cggactgggc gagcggagag agcggttcct
     1021 ccgggccgcc cgcctccgcg ggcaggaaca atcggtgata accgagggac ttcaatgtcg
     1081 ccatcgtcgt gaagaagggg gacccgggag cgatgcgctc cgtcggggtc atccggtcga
     1141 gggcccacgc cgccggccgg agtacctcca cggcgatccg gtgggcctgc tctcgaagta
     1201 ccagttcgtc ctcccccagc cccgccaggt cgaggaacag tccgtcgttc ccgtgtccca
     1261 tggggagaac ctacgccggc ggaactcgat ctcctagggt ccaaagtccc gaacctacgg
     1321 gccacggtgt agccgaagga cgaggccagc caccacaggc agttcgctgc gaagctctgc
     1381 agcggggcgt ggcacatccc gaagtcctct ttcgtctggc ggatcgcttc ttcgggagcg
     1441 ccgcctcgga ggccatggtg atgcttgtca gcccgtagtg tccgaaaact cgattccgca
     1501 cctcagacgc gcgtagggct tgctcagcgg ccggacgaat gctccaggct agatctctcc
     1561 gctgtcaaac gaagaggggt acgcatggga gaccaagtcc gagaccacgt cgtcgtggat
     1621 tctttcccat cggctccaac gttcagccct cgtcggcagc gcgagtgcag ctgagcccgg
     1681 acgccgccaa cgactccccg aggaacgccg ccgcctggcg tgaccacaga tagcccaggt
     1741 ggtttcccga gtaccagctg atgcgcggct tgtcccagtg ctcccacagc ctgcgcgcct
     1801 gctccggcat ggccaggcgg tcgccatagc ctgcgaagac gtaccgatga tcgcgcggca
     1861 ccttcgactc gaagctcatc ggcgacacca ctcggtagac gtcttccgct acgccaccca
     1921 tgattcggtg ctcgatcgat cgagctcgga tgtggaccgg gctgtgctcg tggaagagcg
     1981 ccgggaagtc caccaccggg acgccggcca ccacggcgtc gaacccttcc tcgatgccag
     2041 cgaggagcga gacgacgtag cccccgagcg agactccgta caacccgatg gacgtggctc
     2101 cctgggccct gatccacgat acgaggcgcc ggatgtccca cactgcctgc gtcaacccgt
     2161 gaacggtatt catcatgtcg aaggag
//
LOCUS       DASRMJ010000137         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_483554, whole genome shotgun sequence.
ACCESSION   DASRMJ010000137 DASRMJ010000000
VERSION     DASRMJ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483554"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..1647)
                     /gene="gabT"
                     /locus_tag="VFD74_03875"
     CDS             complement(334..1647)
                     /gene="gabT"
                     /locus_tag="VFD74_03875"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZK48723.1"
                     /translation="MTNQELKQARDKNVPRGPFNVTGLFAAKALNATITDVEGRDYID
                     FAGGIGVMAVGHCHPKVVAATQDQSALFSHTCFHVVMYEAYVELARKMNEAAPGDFDK
                     KTILLNSGAEAVENAVKIARYATGRDAVIAFEDAFHGRTLMTMSLTSKTMPYKKGFGP
                     FAPEIYRLPYAYCYRCPLALEYPGCGVACADLLTDAFKNYVGADEVAAVIAEPVLGEG
                     GFVVPPKEYFPKVKKICEDNGIVFIADEVQSGFGRTGNIFSIDHWGVAPDLITSAKSL
                     AAGYPLSAVTGRAELMDAPHVGGLGGTYGGNPVSCRAALAVFDIIEHDGLLARADVIG
                     ARIRASFDSLAGELEVVGDVRGLGAMVALELVTDRGTKAPAADLTKALVTRALEKGVI
                     MISAGTYGNVIRPLMPLTIDDATLDRGLAIVAESLREVAAEAGLA"
     gene            complement(1644..2633)
                     /gene="speB"
                     /locus_tag="VFD74_03880"
     CDS             complement(1644..2633)
                     /gene="speB"
                     /locus_tag="VFD74_03880"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HZK48724.1"
                     /translation="MKDPSSNTGPTVLPGAAGSMPYVGIASFMKSPIVTAAMLSNFDF
                     VIVGIPFDEGTSNRPGARFGPRGVRAASALYSYEGGAELFDIELGRTILEGARLGDFG
                     DVRIEPLDGDANRAAMTAAVAAVLAAGAIPVCVGGDHSVTAPILRAHAAARGDSLPYL
                     VHLDTHMDFDSYGSRHYHGTPVRRSVEDRSVCGVTQVGIRGFNSGKTDWEEARALGVQ
                     VITCSEFKSAGVQRVLETVPAGAPLYLSIDIDAFDPSIAPGTGTPEPGGLTYLEGREL
                     LRALARHGDVVGIDLVEVSPPYDHAECTSILASRLLLDLMGAVWDNGEEKGSR"
BASE COUNT          415 a          862 c          930 g          474 t
ORIGIN      
        1 ggatcagagc gtcgggtacc tcttcgcgct ccgcggtgcc cgtgttcagg tccactcgaa
       61 ggatctgata cttctccatg ctgcttcctc ccgatctagt ttcgcgccgc gtgtcgccgg
      121 cggtaggtcg tagggccgcg cccgctgcgc gaccctagtg aacgtgtcgc aggatatggc
      181 cccgctcgcg cttcgtcgcg ggtgcgtgtt ccagggtcag catatacgcg gtcgtgtcgc
      241 tctgtacacc cgagttcagc gcatgcgatc ggctcaccgg tcggctcacc cttcacgtgt
      301 gcgcccccgc cgagcgtcgc gcccgcccag tcttcaggcc aatccggctt cggcggcgac
      361 ctcgcgcagc gattccgcga cgatggccaa tccgcggtcg agggtggcgt cgtcgatggt
      421 cagaggcatc agcgggcgga tgacgttgcc gtaggtgccg gcggagatca tgatcacgcc
      481 cttctcgagc gccctcgtca ccagcgcctt cgtcagatcg gcggccgggg ctttggtccc
      541 gcgatcggtc accaactcga gcgccaccat ggcgcccagg ccgcgcacgt cgcccacgac
      601 ttcgagttcc cccgccagtg agtcgaacga ggctcgtatg cgtgccccga tgacgtcggc
      661 gcgagcgagc aggccgtcgt gctcgatgat gtcgaacacc gcgagcgccg cccgacagga
      721 gacggggttg ccgccgtagg tgccgccgag accaccgacg tggggtgcat ccatgagttc
      781 cgcccgtccg gtgacggccg agagcgggta gcccgccgcc aatgacttgg cgctggtgat
      841 gaggtccgga gccacgcccc agtggtcgat ggagaagata ttgccggtgc ggccgaagcc
      901 cgactgcacc tcgtcggcga tgaagacgat gccgttgtcc tcgcagatct tcttgacctt
      961 ggggaagtac tctttcggcg ggaccacgaa gccgccctcg ccgagaacgg gctccgctat
     1021 caccgcggcg acctcgtcag cccccacgta gttcttaaag gcgtcggtga ggaggtcggc
     1081 gcaggccacc ccgcaacccg ggtactcgag agccagcgga cagcggtaac agtaggcgta
     1141 gggcaggcgg tagatctcgg gggcgaaggg gccgaagccc ttcttgtagg gcatggtctt
     1201 cgaggtgagg gacatggtca tgagcgtgcg tccgtggaac gcgtcctcga aggcgatgac
     1261 ggcgtcgcgt ccggtagcgt agcgggcgat cttgacggcg ttctcgaccg cctcggctcc
     1321 cgagttcaag aggatcgtct tcttatcgaa gtcaccggga gcggcctcgt tcatcttgcg
     1381 cgcgagttcc acgtaggcct cgtacatgac cacgtggaag cacgtgtgcg agaagagggc
     1441 ggactggtcc tgggtggcgg ccacgacttt cgggtggcag tgtccgacgg ccatcacgcc
     1501 gatgccgccc gcgaagtcga tgtagtcgcg accttccaca tcggtgatgg tggcgttgag
     1561 ggccttggcg gcgaagagcc cggtgacgtt gaagggtccc cgggggacgt tcttgtcgcg
     1621 tgcctgcttc agttcctggt tggtcatcgt gatcctttct cttctccgtt gtcccagacg
     1681 gctcccatca ggtcgaggag caggcgggaa gcgaggatgg acgtgcattc ggcgtgatcg
     1741 tagggcggac tcacctctac caggtctatg cccaccacgt cgccgtgacg ggcaagcgcc
     1801 cgcagcagtt ctctgccctc caggtaggtg agacctcctg gctcgggagt gcccgtgccg
     1861 ggggcgatgg acgggtcgaa ggcgtcgatg tcgatggata agtagagagg cgcccccgcc
     1921 gggaccgtct ccaacactcg ctggactccg gcgctcttga actcggagca ggtgatgacc
     1981 tgcaccccca gggcgcgggc ttcctcccag tcggtcttgc cgctgttgaa cccgcggatg
     2041 cccacctggg tgacgccgca caccgaacgg tcttcgaccg aacgtcttac cggggtgccg
     2101 tggtagtggc gagatccgta ggagtcgaag tccatgtgcg tatccaggtg gacgaggtag
     2161 gggagggagt ctcctcgggc ggccgcgtgg gcccgcagga tgggcgcggt gaccgagtgg
     2221 tctcctccca cgcacaccgg gatcgctccg gccgccagca ccgccgcgac cgccgcggtc
     2281 atcgcggccc ggttggcgtc accgtcaagc ggctcgatgc ggacgtcgcc gaagtcgccc
     2341 agccgggcgc cctccaggat ggtgcgcccg agttcgatgt cgaagagctc cgcgcctccc
     2401 tcgtaggagt agagggccga ggcggcgcgc acgccgcgcg gcccgaatcg cgctcccggg
     2461 cggttggagg tgccctcgtc gaacgggatc cccacgatta cgaagtcgaa gttggagagc
     2521 atggccgcgg tgacgatggg cgacttcatg aacgaggcga tgccgacgta cggcatcgat
     2581 ccggccgccc cgggaaggac ggtgggacct gtattggatg acggatcttt catcgagagc
     2641 ctccccgtgt ggtcccctcg cagtatagcg ccgcgcaggg c
//
LOCUS       DASRMJ010000138         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_155081, whole genome shotgun sequence.
ACCESSION   DASRMJ010000138 DASRMJ010000000
VERSION     DASRMJ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155081"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFD74_03885"
     CDS             complement(<1..927)
                     /locus_tag="VFD74_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZK48725.1"
                     /translation="MYDFLLNDEQKKLREEVRDFVRWVPRAMIVKMDRDEIRFPKEFL
                     QEAGRRNLLGCRHPKEWGGRHLDWVSACMVMEEIGTLGYIFACTFGVGAELVCDAIIQ
                     HGTEAQKEKYVRPLLRGDLFAAEGLTEPRGGSDFFGTAATAADQGDHFVLNGQKRFIV
                     GAEGADFFLVYARTNPDAKPSEGITAFIVDRGPGVTVEYLYGLMGSRGGGTGRVVFHD
                     VRVPKENAVGKIDGGVEVFNTMMIPERLGTAAMTIGPARAALEVAGGYTTRRKAFGRT
                     INRFEGVSFQVAEASMLLDAARALVYTTARAVD"
     gene            complement(939..2462)
                     /locus_tag="VFD74_03890"
     CDS             complement(939..2462)
                     /locus_tag="VFD74_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZK48726.1"
                     /translation="MNVVEMVDRNARKFAGKDALRYLGRGLTFAELGESARRAAAVLQ
                     SWDVSKGDRVAIMSYNTPSFPIAYYGALVAGAAVVPVNHKLVAPEVDYILAHSGAKVF
                     LFDGALAPLAAKLTSNARQASLDTPAEGHENLDALLDGAPNPSPVKIDDDDMAEILYT
                     SGTTGKPKGCVHSHRNIIMAGITGALAVKYDQDDRVLIAMPIWHSSPLNNWFMAAQYM
                     GATTVLLREYEPLQFLQTIQEERCTVYFGAPISFLAPLQMVPSFGEFDLSSMRAWIYG
                     GGPIDAETARVLMHKYQSDRFYQVYGMTEAGPTGTTLFPSEQVSKAGSIGRTGLPGAD
                     LKVMKSPTEEALAGEVGEIWLTADSMMVGYYDDPQATEQAFVDGWYRTGDAARVDEDG
                     YLFIVDRIKDMIVSGGENVYSKEVEDAIASHPEVAEVAVVGKPHPVWGETVAALVVPK
                     KNGGLTVDDLKSFLAERLAKYKIPRVFRFVDALPYTATGKVMKYQLRDQLRNEPETD"
BASE COUNT          491 a         1032 c         1043 g          535 t
ORIGIN      
        1 gtccaccgcc cgggcggtgg tgtagaccag cgcccgggcg gcatccagga gcatggaggc
       61 ctcggccacc tggaagctca cgccctcgaa gcggttgatc gtccggccga aggccttgcg
      121 gcgggtggtg tagccgccgg cgacctccag ggcggcccgg gcgggcccga tggtcatggc
      181 ggccgtcccg aggcgctcgg gaatcatcat ggtgttgaac acctccaccc cgccgtcgat
      241 cttgccgacc gcattctcct tcgggaccct cacgtcgtgg aacaccaccc ggccggtgcc
      301 gccgcccctg gagcccatca ggccgtagag gtactcgacg gtgacgcccg ggccccggtc
      361 gacgatgaac gccgtgatcc cctcggaggg cttcgcgtcg ggattggtcc ttgcgtagac
      421 caggaaaaag tccgccccct cggcgcccac gatgaagcgc ttctggccgt tgaggacgaa
      481 gtggtcgccc tggtcggcgg cggtggcggc ggtgccgaag aagtcagacc cgccgcgtgg
      541 ctcggtgaga ccctcggcgg cgaagaggtc gccgcgcagg agcggccgaa cgtacttctc
      601 cttctgggcc tcggtgccgt gctggatgat ggcgtcgcag accagctcgg cgcccacccc
      661 gaaggtacag gcgaagatgt agccgagggt cccgatctcc tccatcacca tgcaggcgct
      721 gacccagtcc aagtgccggc cgccccattc cttggggtgg cggcagccca gaagattgcg
      781 gcgccccgcc tcctggagga actccttggg gaagcggatc tcgtccctgt ccatcttcac
      841 gatcatggcc cgggggaccc agcggacgaa atcgcgaacc tcctcgcgga gcttcttctg
      901 ttcgtcgttc agcagaaagt cgtacatgcc tcgccccttc agtcggtctc gggctcgttc
      961 ctgagctggt cgcgaagctg gtacttcatc accttccccg tggcggtgta cggcagcgcg
     1021 tcgacgaagc ggaagacgcg ggggatcttg tacttggcga gcctctcggc gaggaagctc
     1081 ttgaggtcgt ccaccgtcag gccgccgttc ttcttcggca ctaccagggc ggccacggtc
     1141 tcgccccaga cggggtgcgg cttgcccacc accgccacct ccgccacctc cggatgggag
     1201 gcgatggcgt cctcgacctc cttcgagtac acgttctcgc cgccgctgac gatcatgtcc
     1261 ttgatccggt caacgatgaa caggtacccg tcctcgtcga cccgggcggc gtcgcccgtg
     1321 cggtaccagc cgtccacgaa ggcttgctcg gtggcctggg ggtcgtcgta atagccgacc
     1381 atcatgctgt cggccgtgag ccagatctcg cccacctcgc cggccagagc ctcctcggtc
     1441 ggactcttca tcaccttgag gtcggcgccg ggcagcccgg tgcggccgat ggagcccgcc
     1501 ttactcacct gctccgaggg gaagagggtg gtgccggtgg gcccggcctc ggtcatcccg
     1561 tagacctggt agaagcggtc gctctggtac ttgtgcatca gcacccgggc ggtctccgcg
     1621 tcgatggggc cgccaccgta gatccaggcg cgcatcgacg agaggtcgaa ctcgccgaag
     1681 ctcggcacca tctgcagcgg cgcgaggaag ctgatgggcg caccgaagta gacggtgcac
     1741 cgctcttcct ggatcgtctg gaggaactgc aggggttcgt actcgcgcag gagcacggtg
     1801 gtggcgccca tgtactgcgc cgccatgaac cagttgttca ggggcgagga gtgccagatg
     1861 ggcatggcga tgagcacccg atcatcctgg tcgtacttca ccgccagggc gccggtgatg
     1921 cccgccatga tgatattgcg gtgcgaatgc acgcagccct tgggcttccc ggtggtgccg
     1981 gaagtgtaga ggatctcggc catgtcgtcg tcgtcgatct tcaccggcga cggattgggc
     2041 gccccgtcga ggagggcgtc gagattctcg tggccttcgg ccggcgtgtc gagggaggcc
     2101 tgcctggcgt tcgaggtgag cttggccgcg agaggggcga gggcgccgtc gaagaggaaa
     2161 accttcgcgc cgctgtgggc caggatgtag tccacctcgg gggcgacgag cttgtggttc
     2221 accggcacca ccgcggcgcc ggccaccaat gcgccgtagt aggcgatggg gaaggacggc
     2281 gtgttgtagc tcatgattgc cacccggtcg cccttggaga cgtcccagga ctggagcacc
     2341 gcggccgcgc ggcgcgcact ttcaccgagc tcggcgaagg tcagcccccg gccgaggtag
     2401 cggagggcgt ccttcccggc gaacttcctg gcgttgcgat cgaccatctc gacgacgttc
     2461 atgggcaatc ctttccgcgc cggccacagc cacgggcgtg gtgtcggaga accaccgcgc
     2521 catcctctaa tcgcgcactc taaccgtact tcccagtgaa ccgtctctgg cgacagttca
     2581 ttctccgatc aggcaacaag ccgggcctcc tcgcagccgt gcttctggat gagcggccgg
     2641 aagcccggta aacattggtt cggtcaagag ttccggggac gcggaggcaa agcggcacgg
     2701 gctgagcacg cccgcgtcgg gtaccaatgc cctgctctcc acgcgggaga cggccccaag
     2761 ggtgatgcgt gcggcgtctg cggcggggga ctgttctccg gcacaatgca cgcggctggc
     2821 cacatgtccg aaggcgtccg gatccggccg tcagcgacgc ggtcgaaacg aggtgtcgaa
     2881 gtccgagacg cggccgggct cacaaggatc agccgtgggc tcatggatga gcagattcag
     2941 cggctccgcg ttccccgtcc atcaataagt aaccatgtcg cccctccggc gagatcctcc
     3001 cccgccggtt cgatccggct gacgggcatc caggctctat tggcgacatg ggaacgtcgc
     3061 aatacgctct gcgtcaacag tttcggtaac ccagtgcact c
//
LOCUS       DASRMJ010000139         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_517811, whole genome shotgun sequence.
ACCESSION   DASRMJ010000139 DASRMJ010000000
VERSION     DASRMJ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517811"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /gene="mobB"
                     /locus_tag="VFD74_03895"
     CDS             <1..647
                     /gene="mobB"
                     /locus_tag="VFD74_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HZK48727.1"
                     /translation="GPNAAEAGPASPAGAAQADAAAATHLAPRVRSFMERVTVPTVSF
                     VGKKKSGKTTVLTGVIAELVRRGRRVAVLKHDLHGFDVDIPGTDSYRLREAGAMVAGI
                     SSPTMYALVHKPRTEPSLEGLIAHIGESVDVVVTEGFKREAAPKIEVSRRERSTARVC
                     GEEELIGVASDQRFADVSVPQLDLDDFEGLADLVEARVINGWPGCRRGDGTEGP"
     gene            644..1600
                     /gene="moaA"
                     /locus_tag="VFD74_03900"
     CDS             644..1600
                     /gene="moaA"
                     /locus_tag="VFD74_03900"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZK48728.1"
                     /translation="MSGRKVDYLRLSVTDRCNFRCSYCMPAEGVDFRPHEEIMTYEDI
                     VFFVRIAARVGISKIRLTGGEPLVRKGLPSLVSQLSALEGVDDISLTTNGVLLPKFAA
                     DLKAAGLRRVNISIDSLDPDRFRLLTRGGSLNLALAGVEAAFRHRLEPVKINVVMLPE
                     VVDEVEAFAGLTRERPMHVRFIEWMPVGGCGPQGAGDSITKDQLTAAFAHVGELVPVA
                     SPGGSGPARYFRLPGHAGTIGFISSMSDHFCGDCNRLRLTADGRLKSCLFSDDEVDVM
                     AAVRARDELGTLAAIHASLGGKTFDKNRALRPNVRGMSQIGG"
     gene            1602..2099
                     /gene="moaC"
                     /locus_tag="VFD74_03905"
                     /pseudo
     CDS             1602..2099
                     /gene="moaC"
                     /locus_tag="VFD74_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
     gene            2238..2702
                     /locus_tag="VFD74_03910"
     CDS             2238..2702
                     /locus_tag="VFD74_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZK48729.1"
                     /translation="MEGRVVSVNTSPGKGERKKAAESVTFVIDYGIEGDGHAGPWHRQ
                     VSLLAQESIETMVAKGLDVGAGDFAENITTEGIDLVALPIGTRFELGSALVEVTQIGK
                     VCHDRCAIYYQAGDCVMPREGIFVKVIQGGTAAKGDRVSVVGSIFDGEDGSA"
BASE COUNT          466 a          855 c          982 g          446 t
ORIGIN      
        1 ccggccccaa cgccgccgaa gccggccccg ccagccccgc cggcgccgcg caagccgacg
       61 cggccgctgc cacccatctt gccccgcgcg tcaggtcgtt catggagcgg gtgaccgtgc
      121 ccacggtgtc gttcgtgggc aagaagaagt cgggcaagac caccgtgctc accggagtga
      181 tcgcggaact ggtccggcgg ggtcgccggg tcgcggtgct gaagcacgat ctccacgggt
      241 tcgacgtaga catcccggga accgactcct accggctccg cgaagccggg gccatggtgg
      301 cggggatctc gtcaccgacg atgtacgccc ttgtgcacaa gccgcgcacg gaaccgagtc
      361 tggaaggcct gatcgctcac atcggggaat ccgtcgacgt ggtcgtcacc gaaggcttca
      421 agagagaggc cgctcccaag atcgaggtct ccaggcgcga acggagcacc gcgcgcgtgt
      481 gtggcgagga ggagttgatc ggggtggcga gcgaccagcg tttcgccgat gtctcggtgc
      541 cccagctgga cctggacgac ttcgaggggt tggccgacct cgtcgaggcg cgcgtcatca
      601 acgggtggcc gggatgccgg cgcggcgacg ggacggaggg gccgtgagcg gacggaaggt
      661 cgactatctg cgcctttcgg tgaccgaccg gtgcaacttc cggtgttcct actgcatgcc
      721 ggccgaaggg gtcgacttcc ggcctcacga ggagatcatg acctatgagg acattgtctt
      781 cttcgtgcgc atcgcggccc gagtggggat atcgaagata cggctcaccg ggggcgaacc
      841 gctcgtcagg aagggcctgc cgtcactcgt ctcgcagttg tcggccctcg agggcgtcga
      901 cgacatctcc ctcaccacca acggggtgct cctgccgaag ttcgccgccg acttgaaggc
      961 ggccggcctc cgtcgcgtga acatcagcat cgattctctg gacccggacc gctttcggtt
     1021 gctcaccagg ggtgggtcgc tgaacctggc cctggccggg gtcgaggcgg ctttccggca
     1081 ccggttggaa ccggtcaaga ttaacgtggt catgttgccc gaggtggtgg atgaggtcga
     1141 ggccttcgcc ggactcactc gcgagcggcc catgcacgtg cgcttcatcg aatggatgcc
     1201 ggtgggaggt tgtgggccgc agggcgcggg tgactccatc accaaggacc agctgacggc
     1261 tgcgttcgcc catgtcgggg aactcgtgcc cgtggcctct cccgggggat cagggcctgc
     1321 ccgctacttc cggcttccgg ggcacgccgg caccatcggc ttcattagtt ccatgtccga
     1381 ccacttctgc ggcgactgca accgcctgcg tcttaccgcc gacggcaggc tcaagagctg
     1441 cctgttctcc gacgacgagg tggatgtgat ggccgcggtc cgagcccgcg acgagctggg
     1501 cactctggcc gctatccacg cctccctggg aggcaagacc ttcgacaaga acagggccct
     1561 tcggccgaac gtccggggca tgtcgcagat tggagggtga gatggaccag ctacccaccg
     1621 ccgaaacggg agagccgcgt ctcacacacg tggacgagag cggcgcggcc cgcatggtcg
     1681 atgtcggaga caaggcctcg acggtgcgct tcgcgcgcgc cggcgcacgg gtgtggatgg
     1741 cgcctcggac catggcccta ctcaagcggc aggctctccc taagggcgac gtcctcgccg
     1801 tggcgcgcgt cgccggcatc atggcctgta agcgcacggc ggagctggtc ccgctctgcc
     1861 atcccttggt cctcgaccat tcggacatcg aattcgaggt gagggaacag gagtgctgcg
     1921 tggagatcga atgttccacg cgcacggagg gccgcacagg agtggagatg gaggcgatca
     1981 cgggtgtgag tgtggccgca atcaccattt acgatatgtg caaagcggta gaccggggga
     2041 tggtcatcgg agatatccgc ctgctggaaa agacaggcgg caaggaggac tacgtgcgta
     2101 gcgagcggga agagacagag gccggttgtg ctgagacggt cgctgggccg gggccggccg
     2161 cggccggccc cggcccagcg gctgccgggc ctcttgcggc cgcggccgcc gaccttcgcg
     2221 acgaggccgc cagcccggtg gaaggcaggg tcgtctcggt gaacacgagt ccgggcaagg
     2281 gagaacgcaa gaaggcggcc gagtcggtga ccttcgtcat cgactacggc atcgaaggcg
     2341 acggccacgc aggtccctgg caccgccagg tgagtctgtt ggcgcaggag tccatcgaga
     2401 ccatggtggc gaaaggtctc gacgtcggtg cgggcgattt cgccgagaac atcaccaccg
     2461 agggcatcga tctcgtggcc ctgcctatcg gcacgcgttt cgagctcgga tcggctctgg
     2521 tggaggtgac ccagataggc aaggtgtgcc acgacaggtg cgccatctac taccaggcgg
     2581 gtgactgcgt gatgcccagg gaaggcatct tcgtgaaggt catccaaggc ggaacggctg
     2641 ccaagggcga cagggtgtcg gtcgtcggca gcatcttcga cggggaggac ggctcggcat
     2701 gaaccttccg ggtgtgggca ttcgagcggc agtggtcact gtgagcgac
//
LOCUS       DASRMJ010000140         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_89213, whole genome shotgun sequence.
ACCESSION   DASRMJ010000140 DASRMJ010000000
VERSION     DASRMJ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89213"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFD74_03915"
     CDS             <1..413
                     /locus_tag="VFD74_03915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK48730.1"
                     /translation="TSRAAGDHTGGQALGTETFPPQPESLAPIRAVVDRWAREVGLEE
                     AQTEGLTLAVSEACANAIDHPREKGDISLGARRHPDRFTIDVSHPGEFRVRGSQDRQH
                     RGLGLPLMIALADEVSFSCLPKGGTRVSLSVLLR"
     gene            653..>2102
                     /locus_tag="VFD74_03920"
     CDS             653..>2102
                     /locus_tag="VFD74_03920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006278932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HZK48731.1"
                     /translation="MNHNEAVSFIWSVADLLRDSFKRGKYQDVILPLTVLRRIDSVLA
                     PTKESVLETNAKYGGKLDNLDPLLRRASGYAFYNTSRYDFDRLVADPPNLAANLRNYI
                     AGFSPNMREVIERFDFDNTISKLVESGLLYLVMERFKNVDLGPASLDNHAMGTVFEEL
                     IRKFNEALNENPGEHFTPRDVVSLMVDLMIAGDEVKLSEPGRLVKVYDPCCGSGGLLT
                     ITKEHIHASIGPEVGVVLFGQEVNPETFAIAKSDMYMNDPAGRDADNIAFGTVLGDDR
                     HAGSHFDYVIANPPYGKDWKRDEVAVKAEYDRGFAGRFGPGLPSISDGQMLFLEHMIA
                     HMQKPADGGSRMAIIMNGSPLFTGDAGSGPSEIRRWILENDLLEAIVALPEQLFYNTG
                     IATYVWVVTNRKTPERSGKVLLVDATSFWTPMRKSLGNKRREIPAERHAEILGLFAAY
                     EEGKHCRIYPTAHFGYRKITVERPLRLNFQASP"
BASE COUNT          389 a          754 c          611 g          348 t
ORIGIN      
        1 tgaccagccg ggctgcaggt gatcataccg gcggccaggc gctcggcaca gagaccttcc
       61 caccccagcc cgagtcgctc gctcccatcc gggccgtggt ggataggtgg gctcgggagg
      121 tggggctaga agaggcacaa accgagggcc tcaccctggc ggtatccgaa gcctgcgcca
      181 acgcgatcga ccacccccgg gagaagggcg atatcagcct gggggcccgg cgccacccgg
      241 atcgcttcac cattgatgtc tcgcatccag gtgagtttcg agtgaggggc agccaggata
      301 gacagcatcg cgggctgggc ctgcccctga tgatcgcctt ggcggatgag gtcagcttct
      361 cctgccttcc aaagggcggc acccgcgtct ccctctcggt gctgctgagg tagagggtgg
      421 ggtggcctcc aggccgctgg tgaattcctg ggacgcatcg gacgtccgtc gctcccgcca
      481 tccacgacca ccatggcctg atggtgccca tgccggccca tgcagccctg ggcggaccgc
      541 ggcccagttc tccccctcac gcgcccggca tccagcgact ggattcgcgc cgacaccctc
      601 tcctgcaata gaataggaat tcgtctgccc gacaaccgct gagggagagc tcttgaatca
      661 caacgaggcc gtcagcttca tctggtccgt ggccgacctt ctgcgggact ccttcaagcg
      721 tggcaagtac caagacgtca tcctgcccct gacggtcctc cggcgcatcg acagcgtcct
      781 cgctcccacc aaggaatcgg tgctcgagac caacgccaag tacggcggga agctcgacaa
      841 cctcgacccg ctcctccgca gagcctccgg ttacgccttc tacaacacca gtcgctacga
      901 cttcgaccgc ctggttgccg acccgcccaa cctcgccgcc aacctgcgca actacatcgc
      961 cggcttcagc cccaacatgc gcgaggtgat cgagcggttc gacttcgaca acaccatcag
     1021 caagctggtc gagtccggcc tcctctacct ggtcatggag cgcttcaaga atgtcgacct
     1081 gggtccggcc tcgctcgaca accacgccat gggcaccgtg ttcgaggagc tcatccgcaa
     1141 gttcaacgaa gcgctcaacg agaacccggg cgagcacttc accccacggg acgtggtctc
     1201 gctgatggtc gacctcatga tcgcgggcga tgaggtcaag ctctccgagc ccggtcgcct
     1261 ggtgaaggtc tacgacccct gctgtgggtc cggcggcctg ctcaccatca ccaaggaaca
     1321 tatccacgct tccatcggcc ccgaggtggg cgtggtgctc ttcggccagg aagtgaaccc
     1381 cgagaccttc gccatcgcca agagcgacat gtacatgaac gaccccgccg gccgcgacgc
     1441 cgacaacatc gccttcggca ccgttctcgg cgacgaccgt cacgccggct cccacttcga
     1501 ctacgtcatc gccaatcccc cctacggcaa ggactggaag cgagacgagg tcgctgtgaa
     1561 ggcagagtat gaccgtggct tcgccggtcg cttcggtccc ggcctgccct ccatctccga
     1621 cggacagatg ctcttcctcg agcacatgat cgcccacatg cagaagcccg ccgatggcgg
     1681 ctcgcgcatg gccatcataa tgaacggctc gcccctcttc accggggatg cgggctccgg
     1741 cccctcggag atccggcgct ggatcctcga gaacgatctg ctcgaagcca tcgtcgctct
     1801 gcccgagcag ctcttctaca acaccggcat cgccacctac gtctgggtgg tcaccaaccg
     1861 aaagacccca gagcggagcg gcaaagtcct cctcgtggat gccacctcct tctggacccc
     1921 catgcgcaag agtctcggga acaagcgccg ggagattccg gccgagagac acgcggagat
     1981 cctcgggctg ttcgcggcct acgaggaggg caagcactgc cgcatctacc cgaccgccca
     2041 cttcggctac cggaagatca cggtcgagcg ccctctgagg ctgaacttcc aggcgtcacc
     2101 cg
//
LOCUS       DASRMJ010000141         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_47011, whole genome shotgun sequence.
ACCESSION   DASRMJ010000141 DASRMJ010000000
VERSION     DASRMJ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47011"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..470
                     /locus_tag="VFD74_03925"
     CDS             93..470
                     /locus_tag="VFD74_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZK48732.1"
                     /translation="MTIRLKRAYEAPSPVDGVRILVERLWPRGLSKEKADVDVWLKEV
                     APSTELRRWFGHDAAKWEEFRRRYRDELRERPEGLERLRSLVAAGPVTFAYGSRDQEH
                     NAATVLKELLERESAEEQAGKAA"
     gene            796..957
                     /locus_tag="VFD74_03930"
     CDS             796..957
                     /locus_tag="VFD74_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZK48733.1"
                     /translation="MQKWECKACPYVYDPELGDPDNGIPPGTPFEDLPDDWLCPECVL
                     GKEFFEPFE"
     gene            1012..1863
                     /locus_tag="VFD74_03935"
     CDS             1012..1863
                     /locus_tag="VFD74_03935"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZK48734.1"
                     /translation="MSGAPGNVGTELVKLLSARGHQVRVLIRRPESAERLPFSGVETA
                     FGDLTDRESLERSLAGVDRVFVNSAVGPAILAQKNLIDAAEQAGVRHIVKLSWIGASA
                     HLVAVPFSRWHAEVESHVKASGVACTILRASAFMQNYLNYITMPHANTVYGAAGEGKA
                     SLVDARDVAAVAAGVLAEGGHEGKLYEVTGPEALSNAAVAAIISKVTGRHVRSVNVSL
                     DQLTDNYLRLGWPDAWAQELVAIQELRAGGHLATGTDVVERVAGKKATTFEEFVRECV
                     VSSPLET"
BASE COUNT          359 a          649 c          774 g          335 t
ORIGIN      
        1 ggggctgggg cgggccgcct gagaccgcgt gcccatgggt ctgcctccgt gggtgacgta
       61 ggcgtgctcc gaggacagaa agaaggaagg ctgtgaccat ccgtttgaag cgggcctatg
      121 aagctccgtc acctgtcgac ggcgtgcgga tcctggtcga gcgcttgtgg ccgcgtgggc
      181 tcagtaaaga gaaggccgac gtggacgtct ggctcaaaga ggtggctccg agcaccgagc
      241 tgcggcgctg gttcggccac gacgccgcca agtgggagga gttccggcgc cgctaccgtg
      301 atgagctgcg cgagcggccc gaggggctgg aacgactgag gagcctcgtc gcagcgggtc
      361 ccgtgacgtt cgcgtacggg tctcgcgacc aggagcacaa cgccgccacg gtgctcaaag
      421 agctcctcga gcgcgagagc gcagaggagc aggccgggaa ggccgcatga gcggccgctt
      481 ggccccggac ccgacgagat ggcgcagagc gtaatgaggt tcgcgcttca ccccgaccac
      541 cgctccggct cgacaccacc gtcctcgtgc ctcggcgaag cgcggtgaac gcggtcgtgc
      601 gctgggacgc gcccgtgtat ctgcgcgtcc tcgtcgtgga cgggtcggcg atggacgacg
      661 tggggggagc cagctgcata gcggcgccac gatggcaagg ccgcggtcgg ccgtggatag
      721 ctcggtgcga cgtgcgggaa caggaacaag aaagcacgac ggctggcaga gaccagccct
      781 acgaaggtga tcagcatgca gaagtgggag tgcaaggcat gcccgtatgt gtacgacccg
      841 gagctcggcg acccggacaa tgggatccct cccgggacgc ccttcgagga cctgcctgat
      901 gattggcttt gcccggaatg cgtcctcgga aaagagttct tcgaaccttt cgaatagcgc
      961 gcggcggtgc gtgccgagac gcgatggtga agggccggag ggatgatcct cgtgagtggc
     1021 gcgccgggga atgtgggcac ggagctggtc aagctcctct cggcgcgcgg ccatcaggtg
     1081 cgcgttctga tccgcaggcc ggaatcggcc gagagactcc cgttctcggg tgtggagacg
     1141 gcgttcggtg acctcacgga tcgcgagtcg ctggagcgtt ccctggccgg ggtggaccgc
     1201 gtcttcgtca actcagcggt cgggccggcc atcctcgcgc agaagaacct catcgatgcc
     1261 gccgagcagg ccggcgtgcg ccatatcgtc aagctctcgt ggatcggcgc ctcggcgcat
     1321 ctcgtcgctg tgcccttcag tcgctggcac gccgaggtgg agagccatgt gaaggcttcc
     1381 ggggtcgcgt gcaccatcct gcgggccagt gcgttcatgc agaattatct caactacatc
     1441 accatgccgc acgcaaacac cgtctacggt gcggctgggg aggggaaagc cagcctcgtc
     1501 gatgctcgcg acgtcgcggc cgtggccgcc ggcgtcctag cggagggcgg ccacgaagga
     1561 aagctctacg aggtcacggg gcccgaggcg ctctccaacg ccgcggtggc tgcgatcatc
     1621 tcgaaggtca ccggccggca cgtccgctcg gtgaacgtga gcctggatca gctgaccgac
     1681 aactaccttc gcttgggctg gccggacgct tgggctcagg agctcgtcgc catccaggag
     1741 ctgagggcgg gaggccatct cgcgacgggg accgacgtcg tcgagcgggt ggccgggaag
     1801 aaggcgacga ccttcgagga gttcgtgcgc gagtgtgtgg tgtcaagccc tttggagact
     1861 tagccgcgaa ataggtgtgg atctccgcgg tgcatcacga acggaatccg ttgccccagc
     1921 gactggtgcg gcctaacctc gttgaacgtc gtgaggaagt cctcgatctc cgccgcaaca
     1981 gtggccgccg gcgcggtttc gctctggacg cggtcgcgta gctgacgaag acctgcgccc
     2041 gagggcgcgg cggcacggtg tcggcggccg gcgaagatgg cctgtacagg gcgcagttcc
     2101 cccgacgcca cacgtca
//
LOCUS       DASRMJ010000142         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_300272, whole genome shotgun sequence.
ACCESSION   DASRMJ010000142 DASRMJ010000000
VERSION     DASRMJ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300272"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFD74_03940"
     CDS             <1..512
                     /locus_tag="VFD74_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZK48735.1"
                     /translation="YLEELAVSTGTCAFVAVIRGAEVFVVASREAPEGIGVSIRVGYR
                     FPLTFGSIGKAIGAFLPPDELEDFLARQTLHFHGQPSEATPVDLDEVRADLAEARAKG
                     YGVDLGRVQAGVHAVSAPLLASREPHRPQGVVTLIGTFPAEEAEAHGERVAAKAREIS
                     ALFGPLLGG"
     gene            complement(542..1260)
                     /gene="rhuM"
                     /locus_tag="VFD74_03945"
                     /pseudo
     CDS             complement(542..1260)
                     /gene="rhuM"
                     /locus_tag="VFD74_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(1383..>3604)
                     /locus_tag="VFD74_03950"
     CDS             complement(1383..>3604)
                     /locus_tag="VFD74_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK48736.1"
                     /translation="GSSQAVAYAGANRIVRNHALVLLLLAPYLVLLTKLGSDMLIRRP
                     VRGLVAAATAVRDGDLSVRTGLTGAGGEIGQLAQVFDSMAETIEHRTAAAEQSETRYR
                     ALVESTPNGIIVHQDGRIIFANEAAARIGGAPAPADLMGRSVMEFVHPDSLEIARHNI
                     EEAYRTGEPSEMTEERFVRLDGTEIDVEVVAIPLIFEGRPAISTIVTDVTDRRQALDE
                     LLQLRKAVDASGEVIFLTDRDGLITFINPQFTQTYGFTAAEVVGKETARVLKSGAMKD
                     EDYAYFWQTLLDKRIVKGELVNKTKDGRVLTVEGSANPIVNERDEVVGFLAIQRDITD
                     LRLAEEALRESQERAHGAQRMEAVGQLAGGVAHDFNNLLTAIIGFAEIGQARSKGDPA
                     LDEAFREIRASADRAAALTRQLLAFSRKQPLQPRVVDLNVIAESISGLLRRLIREDVE
                     LALRCQSGLRPVAVDPGQIEQLITNLAVNARDAMPGGEKLTIETANVELGEDFTTTHP
                     GAAPGPHVMLAVSDTGTGIDPKVLPHIFEPFFTTKEHGKGTGLGLSTVYGVVKQSGGS
                     IYVYSEPDQGTIFKVYFPVVDRPVDWAPEGRPAQKDRTVGGHETILVVEDEPAVRALA
                     VRVLEGAGYTVLHEGDPTEALKLYDAHGGTIHLLLTDVVLPGMSGKVLADTISAKQGV
                     RPRVLFMSGYTHDAIVHGGRLDASVNFLEKPFTRLGLLDKVRGVLDQPIDGQISLLI"
BASE COUNT          586 a         1163 c         1162 g          693 t
ORIGIN      
        1 cctacctgga ggagctggcc gtgtccacgg gcacgtgcgc tttcgtggcc gtcatccgag
       61 gggccgaggt gttcgtggtg gccagcaggg aagcacccga gggcatcggc gtctccatca
      121 gggtgggcta ccgcttcccg ctcaccttcg gctccatcgg caaagccata ggcgcgttcc
      181 tccccccaga tgagctcgag gacttcctcg cgcggcagac cctccacttc cacggccaac
      241 catccgaggc cacgccggtg gacctggatg aggttcgtgc cgacctcgcc gaggcgcgcg
      301 ccaagggcta cggtgtcgac ctcggccgag tgcaggccgg cgtgcacgcc gtcagcgcgc
      361 cgctgctggc cagccgcgaa ccgcatcggc cgcagggtgt ggtgacgctc atcggcacct
      421 tccccgcgga ggaggcggaa gcccacgggg agcgcgtagc ggccaaggcg cgcgagataa
      481 gcgcgctgtt cgggcctctg ctcggggggt agttcccagc tggtggcggc gacctgaagc
      541 ctcactcgtc ggggctctca tgtttgtcga cgatccgctt cacatcgcgc tcgaaatcgc
      601 tctcgtagtc tcgatcctgg atcactcgga atctggcgta ctcctcctcc gccagccctc
      661 gggctacctc gtgtgaaacg cggccggcat cggtcaggat ctcgtactcg ttgaactgca
      721 ggaacgcgtc gagcttcttg gcccaatcgg ccatgcgcat aggtatctgg cgcgcggcct
      781 ggttctcggc gtggtcgaga aacaccgaca cgatccgctc cagggccttg atgccgaagc
      841 gccgtccctg tttgagtcgc tcgtagtcga gcacgaaccc cttgatgatg aactcccgca
      901 gtgtcctggt ggcccatatg cggaattggg tcgcctgacg actgttcacg cggtagccca
      961 cggcgatgat ggcatcgaga ttgtagcggt caactcccgg gctacggtcc gcccgccctc
     1021 ttgctgaact gttcgaattc ttcgaatagt tgccccttca acaagttcgg cggaccgata
     1081 tatctccttc aggtggtagt tgacggtgtt tacctcgacc ccgaaaagct cggccatgcg
     1141 gcgctggctg atccagaagg tctcatcctc gaagaacact tcgacccgag ccaccccgtc
     1201 gggcgactcg aagagaatga tctcgccctc gtgaggttgg aggttgtcgc cttcgatgat
     1261 caggttctcg gtggcgtcga agttgatgct ctcctcgggg caggggcgca aagtggccag
     1321 gctgggcgtg tacatgatgt cccccccgcg attctcctga catgtcacct gcggggcacc
     1381 actcagatca gaagcgagat ctgcccgtcg atcggctggt cgagcacccc gcgcaccttg
     1441 tccagaagcc cgaggcgcgt gaacggcttc tccaggaagt tcacgctcgc gtctagacgg
     1501 ccgccgtgca cgatggcgtc gtgcgtgtag ccggacatga agagaacgcg cgggcggacg
     1561 ccctgctttg ccgagatggt atctgcgagg accttgccac tcatgccggg gagcacgacg
     1621 tcggtcagta gcaggtggat cgtgcccccg tgcgcgtcgt acagcttgag cgcctcggtg
     1681 ggatcgccct cgtgaaggac cgtgtaaccc gcgccttcta ggacccgcac ggcgagggcc
     1741 cgcaccgcgg gctcgtcctc gaccaccagg atggtctcgt ggccgcctac ggtccgatcc
     1801 ttctgagccg gcctaccctc cggggcccaa tcgaccggcc tgtccaccac gggaaagtag
     1861 accttgaaga tggtgccttg gtccggctcg ctgtagacgt agatgctgcc gccgctctgc
     1921 ttgacgaccc cgtagacggt agagaggccc aggcccgtgc cctttccgtg ttccttggtg
     1981 gtgaagaaag gctcgaagat gtggggaagg accttggggt cgatgccggt gccggtgtca
     2041 gacacggcca acatgacgtg aggtccaggt gccgccccgg gatgcgtggt cgtgaagtcc
     2101 tccccgagtt ccacgttggc agtctcgatg gtcagcttct cgccgccggg catggcgtcg
     2161 cgggcgttga ccgcgaggtt cgtgatgagc tgctcgatct ggccgggatc taccgcgacc
     2221 ggccgtaacc cactctggca ccgaagggcc aattccacgt cttcccgtat caaccgccgg
     2281 aggagtccag agatcgactc ggcgatgacg ttcaggtcca cgactcgcgg ctggagaggc
     2341 tgcttgcgtg agaaggccag taactgccga gtgagagcgg ctgcgcggtc ggctgatgct
     2401 cggatctcgc ggaaggcctc gtcaagggcg ggatctcctt tggagcgtgc ctgcccgatc
     2461 tccgcgaagc cgatgatggc ggtgagcagg ttgttgaagt cgtgagcgac gccgcccgcg
     2521 agttggccaa cggcttccat ccgctgcgcg ccgtgtgccc gctcctggct ttcgcgcaga
     2581 gcctcttcag ccagcctgag gtcggtgatg tcgcgctgta tggccaagaa cccgaccacc
     2641 tcgtcccgct cgttcacgat ggggttggct gagccctcga cggtcagcac ccggccgtct
     2701 ttcgtcttgt tgactagttc gcctttcact atccgcttgt caagcagagt ctgccagaag
     2761 tacgcgtagt cctcgtcctt catcgctcca ctcttcaaga cgcgcgccgt ctccttgccc
     2821 acgacttcgg cggcggtgaa accgtatgtc tgcgtgaact gcgggttgat gaaggtgatc
     2881 aggccgtccc gatcggtcaa gaagatcacc tcgccggagg cgtccacggc ctttcgcagt
     2941 tgcagcaact cgtcaagcgc ttgcctgcga tcggtgacat ctgtgacgat ggtgctgatc
     3001 gcgggtctgc cttcgaagat caaggggatg gccacaacct ccacgtcgat ctcggtgccg
     3061 tccagccgga cgaaccgctc ctccgtcatc tccgagggct cgcccgtgcg ataggcctct
     3121 tcaatattgt ggcgggcgat ctccagagaa tcgggatgta cgaactccat gacgctcctg
     3181 cccatgagat cggccggcgc tggtgcgccg ccgatccgag cggcggcctc gttggcgaag
     3241 atgatccggc cgtcttggtg cacgatgatg ccgttcggtg tcgactctac caacgctcgg
     3301 tagcgggtct ccgactgctc ggccgccgcc gtccggtgct cgatggtctc agccatggaa
     3361 tcgaacacct gggccagctg gccgatctct ccaccggcgc cggtcaaccc ggtgcgaacg
     3421 gacaggtcgc catcccggac ggcggtcgcg gcggcgacca ggccgcgaac cgggcgacgg
     3481 atcaacatgt ctgagccgag tttggtgagg aggaccaggt agggggccaa cagaaggagc
     3541 acgagcgcgt ggttcctcac gatccggttc gctccggcat acgcgacggc ctgagagcta
     3601 cctg
//
LOCUS       DASRMJ010000143         6308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_263900, whole genome shotgun sequence.
ACCESSION   DASRMJ010000143 DASRMJ010000000
VERSION     DASRMJ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263900"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="VFD74_03955"
     CDS             complement(<1..1633)
                     /locus_tag="VFD74_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48737.1"
                     /translation="MVQHGAPLDSDALRANIADTAHTAEIPRRYLPLLDAVDDLRGVR
                     EPLRDLLEEYFHPLRNVDSVLEGLQTLLLRNWSYFERAPQRAERFALAAELVLRLAED
                     SLDEEQLSRLLRLSLTWAAAVAEGPHSEEQAKELADLGSSFATLLPDRPEPFLERDRL
                     VRRVLDRLHKRGGLEAASEPWLDLYLSSLRAGYARIARRLDVAAWIASGEPALRQRRE
                     AEDLFLPVGLSRMLELSSSLPATADVLDYPAHSDVLDEAVGAIHRVSDLEDRFSICLF
                     FLKDDTLGHRQHDVMAELLAVVKTMMEPERRTDFDRVLRNLTSFFHRREGLYPDMRYQ
                     CYQAIGEAIGRAGATRAADHLIEDLLSWRFQYADVRGATEEWQTLANPYHLPNIRCWL
                     HIIESNPAVYDRLAAALDVQLRVGGVFVADTDLFQRDVTRLLNADIKPVYFVVKQLLR
                     TFPVYFNEVGAEGELRSVSTEIDEIMGRQDTLMHFLRKQVHAESSNRLVEFSAAVLEY
                     WHSLDASCLHPFLAPATLAAVRDQRRLAAGPHAVVS"
     gene            complement(1775..2977)
                     /locus_tag="VFD74_03960"
     CDS             complement(1775..2977)
                     /locus_tag="VFD74_03960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZK48738.1"
                     /translation="MPMTLADRANRISHSPTLSIAALAKRMRAAGADVLDFSVGQPDF
                     PTPSCAKEAGKAAIDANRTGYTANEGIPELREAIADKLRLENGLEYAAADIVVSPGAK
                     ATLYFLAMAAFQAGDEVLIPAPFWVSYPEQVALADATPVFVESREENAFQLTPTELEA
                     AITPRTKAVILNYPCNPTGACYQREELEALARVCVEHDLLVISDEIYERLLYDGRVFT
                     SIAALGEEIKSRTVVVNGMSKSFSMTGWRLGYAAGPSPIIDAMSKIQSHSTSNATSIA
                     QWAGLEALRSAEDDVRRMVSAFQERRDIMLAGLLAIPGVTCPRPAGAFYAFPNVSAYF
                     GRRHAGGVIGSALELSAYLLDRARVAVVPGEAFGAPDNLRLSYATSREKIEEGVARIA
                     TALEALGS"
     gene            complement(2979..4325)
                     /locus_tag="VFD74_03965"
     CDS             complement(2979..4325)
                     /locus_tag="VFD74_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZK48739.1"
                     /translation="MNDILGILLLLLLVLLNAFFVAAEFAIVKVRGTRVTELAAEGGR
                     RARVAEHLVSHLDAYLSATQLGITMASLGLGWVGEPALAHLIEPPLDRLGLASPTTLH
                     AASAVLAFAVITFLHIVIGELAPKSVAIRKAEATSLWVAYPLRWFYLVFRPLIWLFNS
                     AANLVLRAIHLTPANEAELAHSEEELRMILTASQAGGHIDEIEQALMRRALTFGERAV
                     GEIMVPRTEMAGLPIDMSIANALEEVAVSNHTRYPVYEEDLDETIGYVHVKELYRADR
                     TKPVRSALRPIGFISETASIEIALKRFQSTRTPLAIVVDEHGGTAGLVTIQDVVEELI
                     GEVQDEFDHDNPLVEPGESDGTFSVDGGARIDYLEEVLGLVVLEAGFPTLGGRVFEQL
                     QRRPRVGDEVEVGNFCARVLEVDGLRITRVLMESCDASEHADGNESHEDGSRRDEE"
     gene            complement(4517..5863)
                     /locus_tag="VFD74_03970"
     CDS             complement(4517..5863)
                     /locus_tag="VFD74_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZK48740.1"
                     /translation="MMRDAAHESMERGALKRLQGERLVSLVERLYANVPLYKGRLDEA
                     GVKPSDVKGIDDLARLPFTYKRDLRDTYPFGMFAVPLKEIVRIHASSGTTGKPTTVGY
                     TRNDIDMWAEMMARTMAAAGVTSDDIVQNAYGYGLFTGGLGAHYGAERLGASVIPISG
                     GNTQKQLMLMQDYGSTILCSTPSFALYIWDVAGEMGIDMASLPLRVGVFGAEPWSNAM
                     RREIEQKLNIKAIDIYGLSEIMGPGVSFECAQVQNGLHVNEDHFLAEIIDPKTGEPLG
                     DGETGELVFTTLTKEGIPLLRYRTGDITRLNVDVCDCGRTFARMDRVSGRSDDMLIIR
                     GVNVFPSQIESVLLMSPEVAPHYQLVVERQGSMDIMDVQVELTPEVFEDAKGAVLTGD
                     GRLALDEWEVLNSLKAKIKNNIKDIVGVTVSVAFKEPGTIERSEGKAKRVIDKRAK"
     gene            complement(5860..>6308)
                     /locus_tag="VFD74_03975"
     CDS             complement(5860..>6308)
                     /locus_tag="VFD74_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZK48741.1"
                     /translation="FNPVPVMKLVEVIPALQTDPSATELATAVTKTLGKTAVPCKDTP
                     GFVVNRILIPMMIDAARVFESGVASAEDIDAAMKLGAGLPMGPLELMDFTGVDIAYHV
                     GNVLYEYTKEQRFSPPTIMRKMVKAGHLGRKTGKGFYDYQQQGGHE"
BASE COUNT         1026 a         1989 c         2180 g         1113 t
ORIGIN      
        1 cagagacaac ggcgtgcggc cccgcggcca ggcgacgctg atcgcggacc gcggccagcg
       61 tagcgggagc gaggaacggg tggaggcacg atgcgtcgag gctgtgccag tactccagca
      121 ccgcggcgct gaactccacc aaccggtttg acgattccgc gtgtacctgc ttgcgcagga
      181 agtgcatgag cgtgtcttga cgccccatga tctcgtcgat ctcagtagag acggaacgga
      241 gctcaccctc ggctcccacc tcgttgaagt agacggggaa ggtccgcagc agctgcttca
      301 ccacgaagta cacgggcttg atatccgcgt tgagcagccg ggtgacgtcg cgctggaaga
      361 ggtcggtgtc ggccacgaag accccgccca cgcgcagctg cacatcgaga gcggctgcca
      421 ggcggtcgta taccgccggg ttcgattcga tgatgtgcag ccagcagcgg atgttcggta
      481 agtggtaggg attcgcgagc gtctgccact cctcggtcgc cccgcgaaca tcggcgtact
      541 ggaagcgcca ggagagcagg tcttcgatga ggtggtcggc tgcgcgcgtc gcccccgccc
      601 ggccgatggc ctctccgatt gcttggtagc actggtagcg catgtccgga tacagacctt
      661 cacgtcggtg gaagaatgac gtgaggttgc gcagcaccct gtcgaagtcg gtgcgccgct
      721 ccggctccat catggtcttc accacggcca ggagttccgc catgacgtcg tgctgccggt
      781 gacccaacgt gtcgtctttg aggaagaaga ggcagatgct gaaccggtcc tcgaggtcgg
      841 acacgcggtg gatcgcaccg accgcctcgt ccagcacgtc cgagtgggcc gggtagtcca
      901 ggacgtccgc cgtcgcgggc aaggacgacg acaactcgag catccggctc aggccaacgg
      961 ggaggaagag gtcttcggcc tcgcgacgct gtctcagtgc aggctcgccc gaggcgatcc
     1021 aagcggccac gtccagtctt cgagctatgc gtgcgtaccc ggcgcggaga ctcgagaggt
     1081 agaggtcgag ccacggttcg gacgcggcct cgagccctcc gcgcttgtgc agtcggtcga
     1141 gcacgcggcg gaccaggcga tcacgctcga ggaagggttc gggccgatcg ggcaacagcg
     1201 tggcgaacga actgcccaag tcggccagtt cctttgcctg ctcctccgag tgagggcctt
     1261 cggccacggc cgcagcccag gtgagcgaga ggcgcagcaa ccgtgacaac tgctcctcat
     1321 cgagggagtc ctcggccaag cgcagcacca actcggccgc gagcgcgaag cgctccgcgc
     1381 gctggggcgc gcgttcgaag tacgaccagt tccgcagcag aagtgtctgg agcccctcga
     1441 gcacggagtc cacgttccgc agcgggtgga agtactcctc gagcaggtcg cggaggggtt
     1501 cacgcacgcc gcgcaggtcg tcgaccgcgt cgagcagggg tagatagcgc cgcgggatct
     1561 cagccgtgtg ggctgtgtcc gcgatgttgg cgcggagcgc gtcggaatcg aggggagcgc
     1621 catgctggac catgtcggct cagcacctac ccacgaaggg tcatggccca acccgatggg
     1681 ccgttcgcac gctcagccgc ttgcgcgcga ccggcccggt tgccgccggc gccgctgaca
     1741 cccgcgcgcc gagccgaccg gggggggtga cttgtcagct gccgagcgcc tccagcgccg
     1801 tggcgatgcg cgcgacgccc tcttcgatct tctcgcgcga ggtcgcgtac gagaggcgca
     1861 gattgtcggg agcaccgaag gcctcaccgg gcaccacggc cacccgggcc cggtcgagca
     1921 ggtaggctga gagttccagg gcggagccga tcacgccgcc ggcgtgtcgg cggccgaagt
     1981 aggcggacac gttggggaag gcgtagaagg ctcccgccgg ccgggggcac gtcactcccg
     2041 ggatggctag gaggccggcg agcatgatgt cgcgccgttc ttggaaggcg ctcaccatgc
     2101 gtctcacatc gtcttcggcg gagcggagcg cctcgagtcc ggcccactgt gcgatcgagg
     2161 tggcgttgct ggtggagtgg ctctgtatct tgctcatggc gtcgatgatg ggcgaggggc
     2221 ccgcagcgta gccgagacgc cagccggtca ttgagaacga cttgctcata ccgttcacca
     2281 cgacggtgcg ggacttgatc tcctctccga gggcggcgat gctcgtgaag acgcggccgt
     2341 cgtagaggag ccgctcgtag atctcgtccg agatcaccag gaggtcgtgc tctacgcata
     2401 cgcgggccag cgcttcgagt tcctcccgct ggtagcacgc cccggtgggg ttgcacgggt
     2461 agttgaggat cacggccttg gtgcgggggg tgatcgccgc ctcaagctcc gtcggcgtca
     2521 gttggaaggc gttctcttcc cgagattcga cgaataccgg ggtggcgtcc gccagggcca
     2581 cctgctcagg gtagctcacc cagaaggggg ccgggatcag gacttcgtca cctgcctgga
     2641 acgcggccat ggcgaggaag tacagcgtgg ccttcgcccc gggcgagacg acgatgtcgg
     2701 ccgcggcgta ttccagcccg ttctcgagtc gcaacttgtc ggcgatggcc tcacgcagct
     2761 cggggatgcc ctcgttcgct gtgtagccgg tgcggttcgc gtcgatggcc gccttcccgg
     2821 cctccttggc gcaggagggc gtggggaagt ccggctgacc cacgctgaag tcgagtacgt
     2881 ccgctccggc cgcgcgcatc ctcttggcga gcgccgcgat gctcagggtg ggagagtgcg
     2941 agatccgatt cgctcgatcg gccagggtca tcggcatcct actcctcgtc cctgcgagag
     3001 ccgtcctcgt gggactcgtt gccgtcggcg tgttcggagg cgtcgcacga ctccatcagc
     3061 acacgtgtga ttcggagtcc gtctacctcc aggacccggg cgcagaagtt gcccacctct
     3121 acctcgtcac cgacgcgggg tcggcgctgc agctgttcga agacgcggcc gcccagggtg
     3181 gggaagcccg cctcaagcac cacgaggccg agcacctcct ccagatagtc gatgcgggcg
     3241 ccgccgtcca ccgagaacgt gccgtcgctc tcgccgggct ccaccagggg attgtcgtgg
     3301 tcgaactcgt cctggacttc gcctatgagt tcttccacga cgtcttggat ggtgaccagt
     3361 cccgcggtgc cgccgtgctc gtccaccacg atcgccagcg gggtgcgcgt gctctggaag
     3421 cgcttgaggg cgatctcgat ggaggccgtc tccgagatga agccgatggg gcggagcgcg
     3481 gagcgcaccg gtttggttcg gtccgcgcgg tacagctcct tgacgtgcac gtagccgatg
     3541 gtctcgtcga ggtcttcctc atagacgggg tagcgcgtgt ggttgctcac cgccacttct
     3601 tccagcgcgt tcgcgatgga catgtcgatg ggcaggccgg ccatctcggt gcgcggcacc
     3661 atgatctccc ccacggctct ctcgccgaaa gtgagcgcgc ggcgcatcag ggcctgctcg
     3721 atctcatcga tatggcctcc ggcctggctc gcggtgagga tcatccggag ctcctcttcg
     3781 gagtgggcca actcggcttc gtttgccgga gtgaggtgga tcgcgcgcag aaccaggttg
     3841 gcggcactgt tgaagagcca gataagcgga cggaagacca ggtagaacca gcggagtggg
     3901 taggcgaccc acagggaggt ggcttccgcc ttcctgatag ccaccgattt cggagccaat
     3961 tcgccgatga cgatgtggag gaaggtgatc accgcgaagg ccaggacggc ggaggctgcg
     4021 tgcagagtcg taggagaggc caggccgaga cggtcgagag ggggctcgat gaggtgggcg
     4081 agcgccggct ccccaaccca gccgagcccc agactggcca tggtgatgcc gagctgggtg
     4141 gccgaaagat acgcgtcgag gtgagagacc aggtgctcgg ccacgcgcgc cctgcggcct
     4201 ccctcggccg ccagttcggt gacgcgggtg ccccgcacct tgacgatggc gaactcggcc
     4261 gcgacgaaga aggcattgag gagaacgagg aggagaagga gaagtatgcc gaggatgtca
     4321 ttcatggcgc tgctccccta gggggggcga ggccctatgt cggccggtac ttcgagggtc
     4381 gtcggaggat ctcatcgtgg tgcgcatagc atagcaaatc ggcgtaagca tgagcattgc
     4441 cgccccggag gccgcgtagc ggcctccggg gcggcgcagt agctgccctg atcgtcgtct
     4501 cgggcggggg gtccggctac ttggctcgtt tgtcgatcac ccgcttggcc ttgccttcac
     4561 tccgctcgat ggtgccgggc tccttgaagg ccacgctcac cgtcaccccg acgatgtcct
     4621 tgatgttgtt cttgatcttg gccttgaggg agttcagcac ttcccactcg tcgagggcga
     4681 ggcgtccgtc accggtcaag acggctccct tggcgtcttc gaacacctcg ggggtcagct
     4741 ccacctgcac atccatgatg tccatggaac cttgacgctc gaccacgagc tggtagtgcg
     4801 gcgccacttc gggcgacatc agcagcaccg actcgatctg gctggggaag acgttgaccc
     4861 ccctaataat gagcatgtcg tcggagcgtc cggagacccg gtccatccgc gcgaacgtgc
     4921 gcccgcagtc gcagacgtcg acgttgaggc gggtgatgtc gcccgtacgg tagcgcagga
     4981 gcgggatgcc ctccttggtg agcgtggtga agaccagttc gccggtctcg ccgtcgccga
     5041 gcggctcgcc ggtcttgggg tcgatgatct ccgccaggaa gtggtcctcg ttcacgtgta
     5101 ggccgttctg cacctgggcg cactcgaagg agactccagg gcccatgatc tcggacagcc
     5161 cgtagatgtc gatcgccttg atgttgagtt tctgctcgat ctcgcgccgc atggcgttgg
     5221 accagggctc cgctccgaag acgcctaccc tcaacgggag ggaggccatg tcgatgccca
     5281 tctccccggc cacatcccag atgtacaggg cgaagctcgg ggtggagcag aggatggtgg
     5341 agccgtagtc ctgcatcaac atcagctgct tctgggtgtt gccgccggag atcgggatga
     5401 ccgacgcccc cagacgctcc gcgccgtagt gtgcgccgag cccgcccgtg aagagtccgt
     5461 agccgtaggc gttctggacg atgtcgtcac ttgtgacgcc ggccgccgcc atggtgcggg
     5521 ccatcatctc agcccacatg tcgatgtcgt tgcgggtgta gcccacagtg gtgggcttcc
     5581 cggtggtgcc ggacgatgcg tggatgcgca cgatctcctt caacggcacc gcgaacatgc
     5641 cgaaggggta ggtgtcgcgg aggtcccgtt tgtaggtgaa gggcagacgc gcgaggtcgt
     5701 cgataccctt tacgtcggaa ggtttcacac ccgcctcgtc cagtcgtccc ttgtacaggg
     5761 gcacgttggc gtacaggcgc tccacgaggg agacgagccg ctccccctgc agacgcttga
     5821 gggcgcctcg ttccatcgac tcgtgtgcgg cgtcacgcat cattcgtgtc ccccttgctg
     5881 ctggtagtcg tagaagccct tcccggtctt tcggcccaga tgtccagcct tcaccatctt
     5941 acgcatgatc gtgggcggac tgaagcgttg ctctttcgtg tattcgtaga ggacgttgcc
     6001 gacgtggtac gcgatgtcca cgcccgtgaa gtccatcagc tcgagcgggc ccatcggcag
     6061 tccggcgccg agcttcatgg ccgcgtcgat gtcctcggcg ctggcgacgc ccgactcgaa
     6121 cacccgcgcc gcgtcgatca tcatggggat aaggatacgg ttgaccacga accccggcgt
     6181 gtccttgcag ggcacagccg tcttgccgag cgtcttggtg acggcggtag ccagctcggt
     6241 ggcggagggg tcggtctgca gagccgggat cacctccacc agcttcatga ccgggaccgg
     6301 gttgaaga
//
LOCUS       DASRMJ010000144         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_452132, whole genome shotgun sequence.
ACCESSION   DASRMJ010000144 DASRMJ010000000
VERSION     DASRMJ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452132"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFD74_03980"
     CDS             <1..304
                     /locus_tag="VFD74_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48742.1"
                     /translation="QILTNFGFTLTRLKSQKVGISALETHRTFFRKRQQQKTKMVIRW
                     PGDGKTVPIGRVKEIRLTLKLCEEDGVTRDTFYAKGVRVDRFINEYRIVLRKLASR"
     gene            complement(333..755)
                     /locus_tag="VFD74_03985"
     CDS             complement(333..755)
                     /locus_tag="VFD74_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZK48743.1"
                     /translation="MPKPEAPASILDASALLALLKDEPGAAAVVDAIEAGAAISTVNF
                     SEVLAKLAERGEHVRSTVAAIQQAVDRADGGLRIESFTEEDGVEAADLRPRSKSQGLS
                     FGDRACLALAARFEVPAITADGDWADLPEVGVAVKLIR"
     gene            complement(742..999)
                     /locus_tag="VFD74_03990"
     CDS             complement(742..999)
                     /locus_tag="VFD74_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48744.1"
                     /translation="MLPQLLPQEPNGVHFVLGRYHEGMAHATKADEAVAKRRRAAAEQ
                     MRGMFAHVAPGVSLADELIADRRVEVRSEELAEADRRRAKA"
     gene            complement(1190..1795)
                     /locus_tag="VFD74_03995"
                     /pseudo
     CDS             complement(1190..1795)
                     /locus_tag="VFD74_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611176.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(1903..3324)
                     /gene="ltrA"
                     /locus_tag="VFD74_04000"
     CDS             complement(1903..3324)
                     /gene="ltrA"
                     /locus_tag="VFD74_04000"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZK48745.1"
                     /translation="MNIGAPWPSLQEAEQRVHAMQTKLHQWAVSEPDRRFDDVHNLVY
                     DRAFLVVAWNVVKTNKGARSAGVDGIAPRSIVAEGDFLAKLRDDLKARRYTPTRVREK
                     AIPKASGKLRRLGIPTAADRVVQSALKLVLEPIFEADFKPCSYGFRPKRRAQDAIAEI
                     HYLGSPSRNYEWVFEADIAACFDEISHSALCERMRARIKDKRIMALVKAFLGAGVLSE
                     DGLNRNTITGTPQGGILSPLLANIALSVLDEHFSRKWEELGPDWTRAKRRRQGVPAYR
                     LVRYADDFVVMVGGTREDAEALWDEVGAVLAPIGLRLSEEKTRVCHIDEGFDFLGWRI
                     QRRRWQGRTDKRAVYTYPSKKALASIMDKVRKTTRRERHRTLAELLRQLNRVLRGWCS
                     YFSHGVSSRTFSYLDHFAWWRVVGWLRKRHHGLNWGTLSRRYLPGWEIRDGRTEMFRP
                     RAVAVTRYRYRGARIPTPWAGAA"
     gene            complement(3923..4276)
                     /locus_tag="VFD74_04005"
                     /pseudo
     CDS             complement(3923..4276)
                     /locus_tag="VFD74_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          697 a         1473 c         1447 g          778 t
ORIGIN      
        1 acagattctt acgaatttcg gctttacact aacccggctc aagagtcaaa aggtaggcat
       61 ttctgccctg gagacgcatc gaacgttctt ccgcaagaga cagcagcaaa aaaccaagat
      121 ggtgatccga tggcctggtg acggaaagac cgttccgatc ggacgagtca aggagataag
      181 gctaacgctc aagctttgcg aggaggacgg cgtaacgcga gacacgtttt atgccaaggg
      241 cgtccgcgta gacaggttca tcaacgaata ccgcatagtc ttgcgaaaac ttgcctcccg
      301 ttgaggggtg atgctcgcga gtcttcgctg gtctaacgga tcagctttac ggcgacgccg
      361 acttccggca gatcagccca gtccccatcc gctgtgattg ccggcacctc gaagcgggcg
      421 gccagcgcga ggcatgcccg gtcgccaaag gacagacctt ggcttttcga gcgtggccgg
      481 aggtcagcgg cctccacgcc gtcctcttcc gtgaaggact cgatacgaag cccgccgtcg
      541 gcgcgatcaa cagcctgctg gatggctgcc accgtcgacc gcacgtgctc tccccgctcg
      601 gccagcttgg ccagcacctc ggagaagttg acggtgctga tggctgcgcc cgcttcgatc
      661 gcatcgacca cagcggcggc gcccggctcg tccttcagca gggccaacag cgccgaggcg
      721 tcgagaatcg acgccggggc ttcaggcttt ggcacgtcgc cggtcggcct ctgccagctc
      781 ttctgagcgg acctcgactc gacggtcggc gatcagctcg tctgccaggc tcaccccggg
      841 agcgacatgg gcaaacatgc cgcgcatctg ctcggccgcc gcgcgacggc gcttggcgac
      901 ggcttcatct gccttggtgg cgtgggccat gccctcatgg tagcggccaa ggacgaagtg
      961 aactccattc ggctcctggg gcaataattg gggcaacatg ccccacagat tccagccgat
     1021 cccagcggga ctcgatccgt cgaaaaccgc ttgaaaccgg gctccacgta gtccagcggg
     1081 tccaacggct cggtctacga atctggaccg tccaagcggg aaaccgaggg tcagctctgc
     1141 cgctgagccc ggtacctgtc aacgacaatg agacaccttc cgttcgaatt tagtgagcaa
     1201 cctcctcggt gtcgggcttg ttgatccacg ctgccgtcgg cagcgtgggc ggcttcggct
     1261 cgccccgcac gaaacgctcg gggttgaccg cgtaggcagc ggcgagcacc gcctggcggg
     1321 cggcgcgggc cttcttggcc cggccgtggt ggacggtcgc gggcgccatc aatccgatgc
     1381 cgcagtggcg gtgctcgtgg ttgtaccagc gaaagaagaa gcggcagaac tcccgcgcct
     1441 gctcaatcga gtcgaagcgg gcggggaagg ccggccgata cttcaaggtg cggaactggg
     1501 cctccgagta tgggttgtcg ttcgaggtgt aggggcggct gtgggtcttg gtgacgccga
     1561 ggtcggcgag caggaaggcg acgggcttgg atcgcatcga ggagccgcgg tcggcatgca
     1621 gggtgagccg cccgcggccg atcgcctgct gctcggccga ttgcgcgatc agcgcttcgg
     1681 cgagggcggc ggactcccgc tcatgcacgg tccaaccgac gacgtagcgg ctgaagacgt
     1741 cgaggatcac gtagaggtgg tagctggtcc acttcgccgg gcccttcagc ttcgagtagg
     1801 ggtcggaccg aggcgcggtg ccgggctctc acccggtccg tctccccggc ccgccctccg
     1861 aaccggacgt gcagctctca ccgcatccgg ctctccacgg gttcatgccg cgcccgccca
     1921 tggcgtggga atccgagcgc cccggtagcg gtagcgggtg accgccaccg ctcgtggacg
     1981 gaacatctcg gtgcggccgt cgcgaatctc ccaaccgggg aggtagcggc ggctgagcgt
     2041 tccccaattc agcccgtggt ggcgcttgcg cagccagccg acgaccctcc accacgcgaa
     2101 gtggtcgagg tagctgaacg tgcgcgagga caccccgtgg ctgaagtagc tgcaccagcc
     2161 gcgcaaaacg cggttgagtt ggcgcagcaa ctcggcgagc gttcgatgtc gctcccggcg
     2221 ggtggtcttg cgtaccttgt ccatgatcga cgcaagcgcc ttcttggacg ggtaggtgta
     2281 gaccgctcgc ttgtcggtcc gaccttgcca gcggcggcgc tggatgcgcc accccaggaa
     2341 gtcgaacccc tcgtcgatgt ggcagaccct cgtcttctct tccgagaggc gcaggccaat
     2401 cggggcgagc accgcgccga cctcatccca gagcgcttcg gcgtcctcac gggtgccgcc
     2461 gaccatgacc acgaagtcgt ccgcgtagcg gacgaggcgg tatgccggaa caccttggcg
     2521 acggcgcttg gcgcgcgtcc agtccgggcc gagttcctcc cacttgcgcg agaagtgctc
     2581 gtcgaggaca gacagggcga tattggccag aagcggcgag aggatgccgc cctgtggggt
     2641 gccggtgatc gtgttcctgt tgagcccgtc ctcggagagg acaccggccc ccaggaacgc
     2701 cttcaccaac gccatgatgc gcttgtcttt gatccgagcc cgcatccgct cacaaagggc
     2761 ggagtgtgag atctcgtcaa aacacgccgc gatgtcagcc tcgaagaccc actcgtagtt
     2821 ccgagagggc gatccgaggt agtgaatctc ggcgatcgca tcctgagcgc ggcgcttcgg
     2881 gcggaagccg taggagcacg gcttgaagtc cgcctcgaag atcggctcga gcaccagctt
     2941 caacgcagac tgcacgacgc ggtccgcggc ggtcgggatg ccgaggcgac ggagctttcc
     3001 cgacgccttc ggtatcgcct tctcccgcac cctcgtgggg gtgtaccggc gggccttgag
     3061 atcatctcgc agcttcgcca ggaagtcccc ctcggccacg atggatcgcg gtgcgatgcc
     3121 atcgactccg gccgagcgtg cacccttgtt ggtcttgacc acgttccagg ccacgaccag
     3181 gaacgcccgg tcgtagacga ggttgtggac gtcatcgaag cgacggtcag gctcgctgac
     3241 cgcccattgg tgcagcttcg tctgcatcgc gtgtacccgc tgctcggcct cttggaggct
     3301 gggccacggt gcgccgatat tcaccggcga cctccgcgct ttgcacctct gctgcgaacc
     3361 cgctggaccc cttcgccatg tgaccggctc tcccggcctc cgactactac gggtcctccg
     3421 ccccatcccg gcgacatcgg ccggcgacgg gccttcccac cggtcagcaa cctgctggcc
     3481 ggggaggggg tcgccgggat ggttcccacg ttcactcgcg aaccgctcga cgggttaggc
     3541 gtccagcttt gcccctgcaa catcgccacg actacgccgc aggcattcac cgtggcctcc
     3601 tggtcggcga catcgaccaa ccggggagtt ctccgcacca tgtggtgtgg atgcgcgctg
     3661 ctgcccggcc ctgatctgcc agattcgagc cggtggatct cttgaggggc tttctgccgc
     3721 tggtttctca cgtacgcctt cccgtcttgc ttgccggacc cggaccgtct ggcagttccg
     3781 atccgtccag tcgttgtcgg ggctgcttcc cgcccgaccc gcccgtcccg gcgggtcaga
     3841 ctgcccccag cttcattcgg cccgctgcga cgggccaatg gcggtgtcct ttcatcaccg
     3901 cacggttcgt gagcgcttcg tggcgctcga tgtcccagct ccaaagttcg ttcgggcggg
     3961 tggccagcaa ctcgggtttc tcataggcgg ggtgggtcag ctggtcgcgg cgctcgcgca
     4021 cctccctgct ctcggccagc agccggtaca tggtccgctc cgagcacagg taggcgccct
     4081 cgtcgagcag cgtcgcccag accgcggcgg gcgaggcgtc gcagaagcgc tcgtcatgca
     4141 gcgcctccag cgcggcatcg cgctcggcct cgctgagcgc acgggccggg cgaggccgcg
     4201 gcctcttcgg ctttggctct ggtgggcgcc ggtgacggta gatcgtcgcc ggcgccgctc
     4261 ccagcgcccg gcaggcgggg cgggtgccga tgaccggcgc cagttcctcg acggtggcct
     4321 cgatcatcgc tcggtgctct cttcggcgcc ctcggtcccg agtaggtcct ccaagagcgc
     4381 tgagacgttt ccctg
//
LOCUS       DASRMJ010000145         5116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_89375, whole genome shotgun sequence.
ACCESSION   DASRMJ010000145 DASRMJ010000000
VERSION     DASRMJ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89375"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VFD74_04010"
     CDS             complement(<1..1212)
                     /locus_tag="VFD74_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48746.1"
                     /translation="MLAVHRSYRAIRFYPAKHPVVVQGFDQLDTILARALSEIGQIRL
                     DVRDNTLLFDGVAVYGGDDPRDNLAELLLVEGLQSIVFHPGIERAETVAFVEALSRAQ
                     ETDRSDQDLAALLWELDLQHLTFTVVDPLLEDHSWEGKGQAIQHLREELVATVSETQD
                     LEIGPPVATTLTPVALDDGALLAGYLATAEELATIEQSMREAPDILEQFVEVLTEVLS
                     AASSDHEIESAAHAISDVLSSYLEWSEFRALARAVERLQALTGGAQGRARVVDGILAA
                     LTTPESLRKAVYALDGARSDSREDLEELLYLLRDLVYPTLIELLTEAGGKVSRKCLLN
                     VCARGAGVPIPLVASRLADPRWYVVRNMVYLLGSLGGPEVMRHLEVGLVHPDERVRRE
                     TVRAMGATGGDQ"
     gene            1504..1755
                     /gene="tatA"
                     /locus_tag="VFD74_04015"
     CDS             1504..1755
                     /gene="tatA"
                     /locus_tag="VFD74_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010314490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZK48747.1"
                     /translation="MPSWIGPWEIGILVIVLLVVFGPKRLPELGSSLGKAITGFKKGL
                     KESEQEFRKAVTEESQVTETPKISQVAETAKSSDEEKTG"
     gene            1794..2801
                     /gene="meaB"
                     /locus_tag="VFD74_04020"
     CDS             1794..2801
                     /gene="meaB"
                     /locus_tag="VFD74_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017600330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZK48748.1"
                     /translation="MRDPRPTPPLSDEQLESARDLADRALSGERRAIARAISLIEDAD
                     PRAREIVRLLYPHTGRARLVGLTGPPGVGKSTLLSGLVHVCRQRGLTVGVVSVDPSSP
                     FTQGAVLGDRIRLTDHFLDPDVFIRSMGTRGHLGGLSETALQAALVLDAAGKDVVFLE
                     TVGVGQSEVEIATIAETVVLALMPGSGDSIQALKAGVMEIPDIIVVNKKDHPSADVTL
                     NEVRSILALSDRDRDWDPPLLATNATTGEGVAELWERITEHREHLQVHGLLDQRRGQS
                     LEKEVLALTRHEVERILAGRSGSDPALAEILRQVRERTLDPLSAAGSILRDVLGIPPE
                     R"
     gene            2880..3143
                     /locus_tag="VFD74_04025"
     CDS             2880..3143
                     /locus_tag="VFD74_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48749.1"
                     /translation="MTIGVTSSRKILHECPLCGAAVELDQDDPLVAAMLSGDSGERGG
                     GRTDRILSALCGCYVLRMDDSALGRLLASVLSMNGGPDKPSSD"
     gene            3243..4424
                     /locus_tag="VFD74_04030"
     CDS             3243..4424
                     /locus_tag="VFD74_04030"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZK48750.1"
                     /translation="MSVSSVILGGARTPFGRSGGALASLTAPELGAVVIRVALRTSRV
                     ADGDVDHAVMGLVLGAGVGQVPSRQATLLAGLPVGLTSETVNKVCASGLRAVSMADQM
                     IRCGDVDVVVAGGMESMSNAPYLLERARFGYRMGDGELVDAMVRDGLIDPIVHVHMGV
                     HNADVSEELHITREEQDAWALRSHRRAVAAIDAGRLVEEIEPVSVRVRKGETIVVATD
                     EAPRRDTSMEALAALRPAFREGGTTTAGNAPGVNDGAGALVVARSGWARERGLAPWAA
                     IRGWATVADEPRYLAKVPAQAALKALAAAGLDAVDVDLWEINEAFASVAVNSVRTLGI
                     DSERVNVNGGAVAFGHPIGASGARILLTLVLELRRRGGGIGVAAICSGIAQGDAVVVE
                     V"
     gene            4488..>5116
                     /locus_tag="VFD74_04035"
     CDS             4488..>5116
                     /locus_tag="VFD74_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZK48751.1"
                     /translation="MDFFLSDEQRDIQRMARTFADERVAPRAGQWDRDHAFPASVLHE
                     LGELGLLGVCVPEEYGGAGADYLSFILVLEEISRGDAGLGVTLAVNTSLGTLPILEHG
                     TESQKREWVPKLATGAVIGAYALTEPASGSDAASISLSARRDGDGYLLNGAKQFVTNA
                     GFAGLFVVMARTGPPGGRAEGITAFLVPAGTEGLVLGREEEKLGLNSSST"
BASE COUNT          786 a         1649 c         1843 g          838 t
ORIGIN      
        1 ctggtcgccg cctgtcgcgc ccatggcacg gacggtctcg cggcgcaccc gttcgtccgg
       61 atggacgagg ccgacctcaa gatggcgcat cacctccgga ccgcccaggg agccgagcag
      121 gtagaccatg ttcctgacca cgtaccatcg cgggtcggcg agacgcgagg ccacgagggg
      181 gattgggact cctgcccccc tggcgcagac gttcaggagg cacttgcgag agaccttgcc
      241 ccccgcttcg gtgagcaact cgatgagggt cggatacacg aggtcgcgca gcaggtacag
      301 cagttcctcc aggtcctctc tgctgtccga ccgggcgcca tccagtgcgt acaccgcttt
      361 gcgcagagat tcgggcgtcg tcagggcggc taggatgccg tccacgaccc gtgccctgcc
      421 ttgcgctccc ccggtgagcg cctggagacg ttcgacggcg cgggcgagtg ctcggaactc
      481 gctccactcc agataggagg agagcacgtc ggagatggcg tgcgccgccg actcgatctc
      541 gtggtcgctc gaagccgcgg agagcacctc cgtgaggacc tccacgaact gctcgaggat
      601 gtcgggagcc tctcgcatcg attgctcgat ggtcgcgagt tcctcagcgg tcgccaagta
      661 gccggcgagc agggccccgt cgtcgagggc gacaggggtc agcgtcgtgg ccacgggcgg
      721 cccgatctca agatcctggg tttccgagac ggtggcgacg agttcctcgc gcagatgttg
      781 gatcgcctgc cccttcccct cccacgaatg gtcctcgagc agtgggtcga cgacggtgaa
      841 agtgaggtgc tgaagatcca gttcccacag gagcgccgcg agatcctggt cggatctgtc
      901 ggtctcctga gcgcgtgaaa gcgcctcgac gaaggcgacg gtctcggccc gctcgatccc
      961 ggggtggaac acgatcgact ggaggccttc caccaagagc agttcagcaa ggttgtcgcg
     1021 cgggtcgtcc ccgccgtaca cggctacccc gtcgaagagg agcgtgttgt cccggacgtc
     1081 gagtcggatc tggcctatct ccgagagggc tcgcgccagg atcgtgtcca gttggtcgaa
     1141 tccctgcacc accaccgggt gcttcgccgg atagaagcgg atggcccggt atgagcgatg
     1201 cacggccaac atgacgtccg cggcgaggtc gcgcggcttc cgcccgtgcg gccggggctg
     1261 tccggatgtg ggtctgccct gctctctcaa tgcgttcgtc ccagccgtgc tccggatggt
     1321 ggggtgcgat actgtgaaga ccgcgactcg acggtcccca gaagggcgcc cgcggccccc
     1381 gcgcccggcg tgcggtggtt gccgcttgct gggaggacta tactatagag ggtcgtctcg
     1441 tcagacgccg tgatctatgc cggccgcccg cgggccggct caccgagtcg gaaggagtca
     1501 accatgccga gttggatcgg gccttgggag atcgggatcc ttgttattgt cctgctggtg
     1561 gtattcggac ccaagagact ccccgagttg gggtcgtcgt tggggaaggc catcaccggc
     1621 ttcaagaagg gtctcaagga gagtgagcag gagttccgca aagcggtcac cgaggagtct
     1681 caggtgaccg agacccccaa gatctctcag gtggctgaga ccgccaagag ctccgacgag
     1741 gagaagacgg gctagataga gtgtcggccg ggcgatccga cgtccggggt cacttgcgcg
     1801 atcctcgtcc cacacctccg ctcagcgacg agcaactcga gagcgcccgc gatctggccg
     1861 accgggcgct ttccggtgag cgcagggcca tcgcgcgcgc catctccctc atcgaggatg
     1921 ccgatcctcg ggcgagggag atcgttagac tcctttaccc ccacacaggg agggctcggt
     1981 tggtcggcct cacggggccg ccgggggtgg gcaagtccac cctcctctcc ggactcgtgc
     2041 acgtgtgccg gcagcgcggg ctgacagtgg gtgtggtctc ggtcgaccca tcgagcccgt
     2101 tcacgcaggg cgcggttctg ggcgaccgta tccgcctgac cgaccacttc ctcgatcccg
     2161 acgtcttcat ccgctctatg ggcacgcgtg gccaccttgg gggactcagc gagacggccc
     2221 tccaggccgc gctggtgctg gacgccgccg gcaaggacgt ggtcttcttg gagacggtcg
     2281 gggtcgggca gagcgaggtg gagatcgcga ccatcgcgga gacggtggtg ctcgctctca
     2341 tgccgggttc cggtgactcg atacaggcgc tcaaggccgg ggtcatggag atacccgaca
     2401 tcatcgtcgt caacaagaag gaccaccctt ccgccgacgt cacgctgaat gaagtgcgca
     2461 gcatcctggc tctcagcgat cgcgatcgtg actgggaccc gccccttctc gccaccaacg
     2521 ccaccaccgg cgagggtgtc gcggagttgt gggagcgcat cacggagcac cgggagcatc
     2581 tccaggtgca tggtcttctc gaccagcggc gcggtcagag cctcgaaaag gaggttctcg
     2641 cgctcactcg gcacgaggta gagcggattc tggccggtcg ttcgggctcc gatcccgcgc
     2701 tcgccgagat actgaggcag gtgagggagc gcacgctcga ccccttgtcg gctgcgggat
     2761 cgatcctccg cgacgttctg gggatcccgc cggagcgcta atagcccgag gctcccggcg
     2821 ctcatgtcgt gggtgcttcc cgccgatact gagggggctg acccccatag gcggaaggtg
     2881 tgacgatagg cgtgacgagc agtcggaaga tcctgcatga gtgcccgctg tgtggcgcgg
     2941 ccgtggagtt ggatcaggat gatccgctcg tggcggcgat gctctcgggt gattccgggg
     3001 aacgtggcgg cgggcgcacg gacaggatcc tgtcggcgct ctgcggctgc tacgtgctca
     3061 ggatggatga cagcgcgctc ggacgtctcc tggcctccgt cctgtcgatg aacggcgggc
     3121 cggacaagcc gtcctccgac tgagtcgccc ccacggcggg ccggacaggt cgtcctccga
     3181 ctgaagagga gaaggagccc cagagtttgc ggcacgcgcc acaagcggta tggtggggcg
     3241 gtatgagcgt gtcttccgtg atactggggg gagcccgcac tccgttcggc cggtcgggcg
     3301 gcgccctggc ttctttgaca gcccctgagt tgggggccgt cgtcatccgc gtcgccctcc
     3361 gcacctcgcg cgtcgccgac ggcgacgtcg atcacgctgt catgggactc gttctgggtg
     3421 cgggcgtggg acaggtgcct tcgcgtcaag ccacgctgct ggcgggcctg cccgtcggcc
     3481 tcacctcgga gaccgtcaac aaggtgtgtg cctcgggtct gcgcgccgtc tccatggccg
     3541 accagatgat ccgctgcgga gacgtggacg tggtcgtggc cgggggcatg gagagcatgt
     3601 cgaacgcgcc ctacctgctc gaacgggcgc ggttcggcta ccgcatgggc gatggagagc
     3661 tcgtcgatgc catggtccgg gatgggctga tcgatcccat cgtgcacgtg cacatgggcg
     3721 tccacaacgc cgacgtctcc gaggagctcc acatcacccg cgaggagcag gacgcctggg
     3781 cgctgcgcag ccaccggcgt gccgtggccg ccatcgacgc cggccgcctc gtcgaggaga
     3841 tagagccggt gtcggtgcgg gtccggaagg gtgagaccat cgtcgtggca accgacgaag
     3901 ctccccggcg cgacaccagc atggaggcgc tggccgctct tcgacctgcc tttcgcgagg
     3961 gggggaccac cactgccggc aacgcccccg gcgtgaacga cggagcgggc gcactggtgg
     4021 tggctcgatc gggttgggcg cgcgagcggg ggctggctcc gtgggcggcc atccgcgggt
     4081 gggccacggt ggcggatgag ccgcgctacc tggcgaaggt gcccgcgcag gccgcactca
     4141 aggccttggc ggccgcgggg ctcgacgcgg tggacgtcga cctgtgggag atcaacgaag
     4201 ccttcgcgtc ggtggcggtg aacagcgtgc gcaccctcgg catcgactcc gagcgcgtca
     4261 acgtgaacgg aggggccgtc gcgttcggcc atccgatcgg cgcgagcggc gcccgcatcc
     4321 tgttgaccct ggtgctcgag ctccggcggc gtggcggggg catcggagtc gcagccatct
     4381 gcagcggcat cgcgcagggc gacgcggtgg tggtggaagt ctagtgggaa gtggagacgg
     4441 cggcgccggc cggttaggcc ggcgccgccg caggaaggca gggggacgtg gatttctttc
     4501 tcagcgacga gcaacgggat atccagcgca tggccaggac cttcgcggac gagcgggtgg
     4561 ccccgcgggc cggtcagtgg gaccgcgacc acgcctttcc cgcgtccgtg ctgcacgaac
     4621 tcggtgagct cgggcttctg ggcgtgtgcg tgcccgagga gtatggcggc gccggtgccg
     4681 actacctcag cttcatcctg gtcctcgaag agatcagccg gggcgacgcc ggcctcggcg
     4741 tcacgctggc ggtcaacacg tcgctgggca cgctgcccat tctggagcac ggcaccgaga
     4801 gccagaagcg tgagtgggtg ccgaagctgg ccaccggcgc ggtcatcggc gcctacgccc
     4861 tgacggagcc cgcctccggc tcggacgcgg cctcgatcag cctgtcggcg cggcgcgacg
     4921 gcgacggata cctgttgaac ggggccaagc agttcgtcac gaacgcgggg ttcgccggac
     4981 tcttcgtggt gatggcgcgc accgggccgc ccggcgggcg cgcggagggg atcaccgcct
     5041 tcctcgtgcc ggcaggcacc gaggggctcg tgctcggccg tgaggaggag aagctcggtc
     5101 tgaacagctc cagcac
//
LOCUS       DASRMJ010000146         4653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_372575, whole genome shotgun sequence.
ACCESSION   DASRMJ010000146 DASRMJ010000000
VERSION     DASRMJ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372575"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFD74_04040"
     CDS             complement(<1..332)
                     /locus_tag="VFD74_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48752.1"
                     /translation="MSRAVDASPEAPPLGAASRPVDRVALDPERVDRAPVARGPVSGR
                     GVVFLGSALDADEREESLREKRAAWQTSGTECDPYSAVLKRLASRCGAPPAWEAGIRE
                     LGCVNVPP"
     gene            complement(402..1493)
                     /locus_tag="VFD74_04045"
     CDS             complement(402..1493)
                     /locus_tag="VFD74_04045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtaA/CmuA family methyltransferase"
                     /protein_id="HZK48753.1"
                     /translation="MTEAERRRDALGVPAVKLMKGAWHMNAKQRVMDVLNGKEVDITP
                     VGCTTTYGVVDLMEACGAERPLADTDPKAMAELAIAGVKHAGFEWIKAMGWDITPMSE
                     AFGCLLGEPKIDLQYSVKGHPYAESLDGLDYPADFLSRGRFPAYAEQFKILKQWAGDD
                     YVVFGESEGAFTCAANLVGTEQFMKWTLKEPEKVHQVLAITKQATIDVANWAFAQGAD
                     YFCMADPTSGPGLMSPKTWEKFVKPVVADIVAHVNGPLVLHICANTDKIIPMMCDTGL
                     AGISIEEKADMKAAVETAKQKGVAVFGNVATATTMFMGTPEEVYQESLAALENGCQFL
                     TPGCGIAPRSPLENVKQLRKARDDYFGIQ"
     gene            complement(1490..2812)
                     /locus_tag="VFD74_04050"
     CDS             complement(1490..2812)
                     /locus_tag="VFD74_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZK48754.1"
                     /translation="MNWSIPTLEDLEHSVWRCTACGTCKVAYEYGPPPKHGEICPAGV
                     EFGFEGYLASKGKIAFARGILSGDLEWDGPLAEAVYRCTLCGGCQSQCQLDHKPYLIE
                     IFESMRRKAVEAGVGPLPSQRVVAQSLATYENPYQAPRRVRTDWTRQFKKAGKPVRTF
                     ADTDAEILFFVGCTGAYNQGARVIPTATASIFQKLGLDFAILGEEERCCGSTAMRIGL
                     TDDFTRIAEANMETFSRLRDEKGMKTIVTSCAGCFRAVKKDYSLADGYAEAMDGIRVV
                     HTVDFLNELYREGRLPLAAGAERVVTYHDPCHTGRHLNKFYVDEDGSEQYVGAYLGLS
                     EDECVYDAPRELLQAIPGVEFREMPRNRANSFCCGGGGGVMTGFPDWATRNASKRIAE
                     GEATGAGEMVSICPFCFFNLGEGARQGESALRVRDLTELIDEALPDSI"
     gene            complement(2841..4277)
                     /locus_tag="VFD74_04055"
     CDS             complement(2841..4277)
                     /locus_tag="VFD74_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZK48755.1"
                     /translation="MGAVALETTRVLDELRGIVGEQNATADKHIRYAYSYDMSFVKPK
                     LPDYVVMASTVEEVQEVLRLANREKIPVVPYTSGTNIGGLCIPERGGILLDLKRMNRI
                     LEIDTEAHYAVIEPGVSHAQLASALFPHDLRFGWPVGPPSASVTACCVSHGIGGLNAR
                     YGLQSQEITSMEVVLPTGELVRVGSCAVQKDAWHSLLPLPQLDGLFKGWLGTTGVVTK
                     LGIGVHPIPPLQKTFTVSATNVEHMYSYMLNLSNYEICDDLTAVSWWLAQVPIPYPFK
                     PKPDDAAEWFCFANTNSFTEKERDARAEIWEKVLREEQAKGSSLKVTEYPEEALKART
                     QLPSQIVGSTKNYCKQGGAGISWPGTFTPAKKWAPVYTEWKKILIDHNLSPSVRMSMY
                     RGVHYGMLRAMIPFSKETPEAIENARHAIVECLKVDLDHGGIPYKPPVDFAIEINKRV
                     DPGYLELLTRVKRFLDPNDIMNPGKLGI"
     gene            complement(4430..>4653)
                     /locus_tag="VFD74_04060"
     CDS             complement(4430..>4653)
                     /locus_tag="VFD74_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48756.1"
                     /translation="EMLETNLQRGVAGIIGAVLAIQGAAWALGIGIGLWLLLERPWIK
                     DADTPGPAAAAAPPGGGGGAGAGPKAAGS"
BASE COUNT          754 a         1557 c         1476 g          866 t
ORIGIN      
        1 cacgggggga cgttgacgca tcccaactct cgtatcccgg cctcccaggc ggggggggct
       61 ccgcagcgtg acgcaaggcg cttgagcacg gccgagtacg gatcgcactc agtcccgctg
      121 gtctgccagg cggcacgctt ctctcgaagg gactcttctc gctcatcggc gtcgagcgcg
      181 gacccgagga agaccacccc gcggcccgac acgggcccgc gggcaacagg cgcccggtcg
      241 acgcgctccg ggtccaaggc cacccggtcc acgggccgcg acgcagcacc gagcggcggc
      301 gcctccgggg aagcgtcgac ggcccgcgac acatgccgcc tctccacact gtctcggggc
      361 ccatggacct ttctcgcctt gcctcgtctc gccactacgt cctactggat accgaagtag
      421 tcgtcgcgcg ccttgcgcag ttgcttgacg ttctccaaag gcgagcgggg ggcgatgccg
      481 cagccggggg tgaggaattg acacccgttc tcgagcgccg ccagcgattc ctggtagacc
      541 tcctcaggag tgcccatgaa catggtagta gccgtggcga cgttaccgaa gacggcgacg
      601 cccttctgct tggcggtctc gacggccgcc ttcatgtccg ctttctcctc gatcgatatg
      661 ccggccagac ccgtgtcgca catcatgggg atgatcttgt cggtgttcgc gcagatgtgc
      721 agcaccagag gaccgttcac gtgggccacg atgtccgcta ccaccggctt cacgaacttc
      781 tcccacgtct tcgggctcat gagaccgggg ccggaggtgg ggtcggccat gcagaagtag
      841 tcggcccctt gggcgaacgc ccagttcgcc acgtcgatgg tggcctgctt ggtgatcgcc
      901 aacacctgat gcaccttctc gggctccttg agcgtccact tcatgaactg ctcggtcccc
      961 accaggttgg cggcgcaggt gaaggccccc tcggactcgc cgaagaccac gtagtcgtcg
     1021 ccggcccact gcttcagaat cttgaactgt tcggcgtagg cggggaaacg cccgcgactg
     1081 aggaagtcgg cggggtagtc gagcccgtcg agactctcgg cgtagggatg acccttgacc
     1141 gagtactgca gatcgatctt cggctcgccc aacaagcaac cgaaggcttc gctcatcggg
     1201 gtgatgtccc accccatcgc cttgatccac tcgaaaccgg cgtgcttgac gccggcgatg
     1261 gcgagttcag ccatggcctt cggatcggtg tcggccagag gcctctcagc gccgcatgcc
     1321 tccatgaggt cgaccacgcc gtacgtggtg gtgcagccca ccggggtgat gtcgacttcc
     1381 ttgccgttca gaacgtccat tactcgttgc tttgcgttca tgtgccacgc ccccttcatc
     1441 agcttgaccg ccgggacgcc caaggcgtcc cggcggcgtt cggcctcggt caaatggaat
     1501 ccggcagtgc ctcgtcgatg agttcggtca gatctcgcac cctgagggcg ctctcgcctt
     1561 ggcgggcgcc ctcgcccagg ttgaagaagc agaagggaca gatggagacc atctcgccgg
     1621 ccccggtcgc ctcgccctcc gcgatccgtt tggaggcgtt cctggtcgcc cagtcgggga
     1681 agcccgtcat gactccgccg ccgccaccac agcagaacga gttggcgcga ttgcgcggca
     1741 tctcgcggaa ctcgacgccc gggatcgcct gcaggagttc gcgcggggcg tcgtacacgc
     1801 actcgtcctc actcagaccg aggtacgcgc ccacatactg ctcgctgccg tcctcgtcca
     1861 cgtagaactt gttcaggtgg cggcccgtat ggcacgggtc gtggtaggtg accacgcgtt
     1921 cggctccggc ggcgagcggg agccgcccct cgcggtagag ttcgttcagg aagtccaccg
     1981 tgtgcaccac acggatgccg tccatcgcct cggcgtaccc atccgctagg ctgtagtcct
     2041 tcttgacagc gcggaagcag cccgcgcacg acgtcacgat cgtcttcatg cccttctcgt
     2101 cgcgcaggcg ggagaacgtc tccatgttcg cctccgcgat gcgggtgaaa tcatctgtca
     2161 agccgatcct catcgccgtc gagccgcagc agcgctcctc ttcgcccaag atggcgaaat
     2221 cgaggcccag cttctggaag atggaagccg tcgcggtggg gatgacgcgc gcaccctggt
     2281 tgtaggcgcc ggtgcaaccg acgaagaaca ggatctcggc gtcggtgtcg gcaaacgtgc
     2341 gaacgggctt ccctgccttc ttgaactgcc tggtccagtc ggtccgcact cgccgaggag
     2401 cctggtaggg gttctcgtag gtggccagcg attgtgccac cacccgttgc gacggcagag
     2461 ggcccacacc agcctccacc gctttccggc gcatcgactc gaagatctcg atgaggtaag
     2521 gcttatggtc gagctgacat tggctctgac atccgccgca gagcgtgcag cggtacacgg
     2581 cctccgccag gggcccgtcc cactccaggt ctccggacag gatgccgcgg gcgaaggcga
     2641 tcttgccttt cgacgccagg taaccctcga agccgaactc cacgcccgcc gggcagatct
     2701 ccccgtgctt gggaggtggg ccatattcgt acgccacctt gcaggtgccg cacgcagtgc
     2761 agcgccacac cgagtgctca agatcttcga gcgtcgggat actccaattc atcgcgtcct
     2821 ccctgccttc accgtacgcc ctagatgccg agcttgccgg ggttcatgat gtcgttcgga
     2881 tcgaggaacc gcttcacgcg ggtcagcagc tccaagtatc cagggtcgac ccgtttgttg
     2941 atctcgatcg cgaagtcgac cggcggcttg taggggatac cgccgtggtc gaggtcgacc
     3001 ttgagacact cgacgatcgc gtggcgcgca ttctcgatgg cttcgggtgt ctccttgctg
     3061 aacgggatca tggcgcggag catcccgtag tgcaccccgc ggtacatgct catgcgcaca
     3121 gagggggaga ggttgtgatc gatgaggatc ttcttccact ctgtgtagac gggggcccac
     3181 ttcttcgcgg gtgtgaaggt gcccggccag gagatcccgg cgccgccctg cttgcagtag
     3241 ttcttggtgg agcccacgat ctggctcggg agctgggtcc tggccttgag cgcttcttcg
     3301 gggtactccg tgaccttcag cgacgaaccc ttggcctgct cctcgcgcag caccttctcc
     3361 cagatctcgg cgcgggcgtc gcgctccttc tcggtgaacg agttggtgtt cgcgaagcag
     3421 aaccactcgg ccgcatcgtc gggcttgggc ttgaacggat aggggatcgg cacctgggcc
     3481 agccaccacg agaccgccgt gaggtcgtcg cagatctcat agttgctcag gttgagcatg
     3541 tacgagtaca tgtgctccac gttcgtcgcc gagacggtga acgtcttctg gagcggcggg
     3601 atgggatgga cgccgatgcc gagcttggtg acgacgcccg tcgtgccgag ccaccccttg
     3661 aagagaccgt cgagttgcgg cagcggcaga aggctgtgcc atgcgtcctt ctgcacggcg
     3721 caggaaccga cgcgcactag ctcaccagtg gggagcacca cctccatgct ggtgatctcc
     3781 tggctctgca ggccataccg ggcgttcaag ccgccgatgc cgtgcgagac gcaacaggcc
     3841 gtcaccgagg ccgacggcgg acccaccggc cagccgaagc gcaggtcgtg cgggaacagg
     3901 gcggaggcga gttgcgcgtg gctcacgccc ggctcgatca ccgcgtagtg ggcttccgtg
     3961 tcgatctcga ggatgcggtt catccgcttg agatccagga gtatcccacc ccgttcgggt
     4021 atgcagagcc ccccgatgtt cgtgcccgag gtatacggca ccaccgggat cttttcccga
     4081 ttggcgaggc gaagtacctc ctgcacctct tcgaccgtac tggccatgac gacgtagtcc
     4141 ggcagcttcg gtttcacgaa ggacatgtcg tacgagtagg cataccggat gtgcttgtcg
     4201 gcagtggcgt tctgctcgcc gacgatgccg cgcagctcgt ccagcacccg ggtggtttcc
     4261 agcgcgacag cgcccatgct caagcctcct atgcctcgac gaggcgatct gtagagccgc
     4321 cacgttcttt cggctccgtc aggtgagtca ccctcacggt ctctatttcc aggatccgtc
     4381 gtggcccgcg ggctaagccc gcgggccacg agcggtcatt cgtgcggggc tacgaccccg
     4441 ctgctttcgg tccggctcca gctccgcctc cgccccctgg gggagctgcc gcggctgcgg
     4501 gaccgggtgt atccgcgtcc ttgatccacg gacgctcaag gagcagccag agcccgatcc
     4561 caatgccgag agcccaagcg gcgccttgga tggcgagcac cgcaccgatg atgcccgcga
     4621 cgccacgctg cagattcgtc tcaagcatct cca
//
LOCUS       DASRMJ010000147         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_264060, whole genome shotgun sequence.
ACCESSION   DASRMJ010000147 DASRMJ010000000
VERSION     DASRMJ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264060"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..898
                     /locus_tag="VFD74_04065"
     CDS             275..898
                     /locus_tag="VFD74_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZK48757.1"
                     /translation="MFGRDEGGRSLKWLSLGDEYTRECPTLLCSRSITAQDVIDELIR
                     LMKTRGMPAHIRSDNEPEFMAKAIHTWLQKAGVGTLFVESGAPWENGYAESFHARLRD
                     EFLKAELFSNLQEAKALGEQWRWEYNHERPHSSLGSLTPSEFAAGRLPSSVVLGAALS
                     FRTIKGGQTPSRSGTGWWRRSPRSSFSRPRRTMWLRSLTLSATKDSC"
     gene            802..1476
                     /locus_tag="VFD74_04070"
     CDS             802..1476
                     /locus_tag="VFD74_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK48758.1"
                     /translation="MVAKVPAVVVQPTSADDVAAVVDFVRNEGLLLSVKCGGHNIAGT
                     SIAQSGVTLDMSRMRSVSVDPQARVAQVDPGCLLGDVDQATQEHGLATVLGFVSQTGV
                     AGLTLGGGWGYLTRRFGWTGDNLEEVEIVTADGKIRTANREEHPDLFWAVRGGGGNYG
                     VVTRFEFRLHEVGPMITGGLIAWSAERAEEVLEAYRDLTSTAPRELTAATVIRLAPPA
                     PFVPEE"
     gene            1498..1818
                     /locus_tag="VFD74_04075"
     CDS             1498..1818
                     /locus_tag="VFD74_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48759.1"
                     /translation="MLVCYSGDAPDSALAPIYGIGDPIANVVTSKPYVAQQSMLDANE
                     PKGPHRYWKTEYIPELPDGFLSAFREGGLAVTSPLFQGVIFHLGGALNERSADDGAAD
                     NRDA"
     gene            1933..2076
                     /locus_tag="VFD74_04080"
     CDS             1933..2076
                     /locus_tag="VFD74_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HZK48760.1"
                     /translation="MNFQLADDDQNRLQAGYRDNFQRLQDIKAEYDPHNLFRVNRNIS
                     PAA"
     gene            2313..2687
                     /locus_tag="VFD74_04085"
     CDS             2313..2687
                     /locus_tag="VFD74_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48761.1"
                     /translation="MRELWEFFSSNREAVLDLTVEHIWLVLAAVSIAVVLGIVLGLLS
                     SYWVSSPVRWSNLFWSYALSRAPASLVASFLYVPSALTILIAWVWLREVPTALTVVGG
                     SVAVLRVFIVNTWGRRRLPQAS"
     gene            complement(2837..>3096)
                     /locus_tag="VFD74_04090"
     CDS             complement(2837..>3096)
                     /locus_tag="VFD74_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZK48762.1"
                     /translation="YEGEIPLTDLFIPYDEAAGHVDQLPDDKDAKIVVYCQSDHMSRI
                     AAAVWAEEGYTNLYNLDGGFVACEKVGYEFIKDGPGAEGSE"
BASE COUNT          558 a          943 c          991 g          604 t
ORIGIN      
        1 ggagcggccc ttcgtcccgg tcgaagatga agtcaagcgc ccagacgtgg ttcctccgct
       61 ccgggcggtg gcgatgacag gcgttctcgc ttgcacccag gcgccgtttc ttgtgctgaa
      121 cgcgcgccaa ccccaagcgc atctaccgcc tctggaagcg cgaaggctac cgggtggcgc
      181 gcgttcagca caagaaacgc cgcctgggct caagcgagaa cgcctgccac cgtcgccgcc
      241 ccgagcataa gaaccacatc tgggcgctga cttcatcttc ggccgggacg aaggcggccg
      301 ctccttgaag tggctctctt tgggcgatga gtacacccgg gagtgcccca ccctgctgtg
      361 cagccggagc atcaccgcgc aggacgtgat cgacgaactc atccgcctca tgaagacccg
      421 cgggatgccc gcccacattc gctctgacaa cgagcccgag ttcatggcga aggccatcca
      481 cacctggctc caaaaagccg gagtcggcac cctcttcgtg gagtccgggg ctccctggga
      541 gaacggctac gccgagtcct tccacgcccg tctgcgcgat gagttcctga aagccgaact
      601 cttcagcaac ctacaagaag ccaaggctct gggagagcag tggcgctggg agtacaatca
      661 cgagcggccg cacagctccc tggggtccct gaccccgagt gagttcgccg ccggccggct
      721 gccctcgagc gtcgtcttgg gggcggcgtt atccttccgg acgatcaagg gtggtcagac
      781 gccgtcgcga tctggaacgg gatggtggcg aaggtccccg cggtcgtcgt tcagccgacc
      841 tcggcggacg atgtggctgc ggtcgttgac tttgtccgca acgaaggact cctgttgagc
      901 gtgaagtgcg ggggtcacaa catagcgggg acctcgatag ctcagtccgg ggtaaccctc
      961 gatatgtcaa ggatgcggtc ggtctccgtc gatcctcagg cccgtgttgc ccaggtcgat
     1021 cccgggtgcc ttctcggaga cgtggaccaa gccacccagg agcacggact cgccacggtg
     1081 ctcggctttg tctctcagac cggagtggcc ggtcttacgc tgggcggcgg ttggggctat
     1141 ctgacccgtc gtttcggctg gaccggcgac aacctcgagg aagtcgagat cgtgaccgcc
     1201 gacgggaaga tccgtacagc caaccgggag gaacatcccg atctgttttg ggccgtgcgc
     1261 gggggaggcg gcaattacgg agtagtgacc cgcttcgagt ttcgtctcca tgaagtggga
     1321 ccgatgatca ctggtggtct tattgcctgg agcgccgaga gggcggagga ggttctcgaa
     1381 gcctaccgag acctgacctc caccgcgccg agagagctga cggcagccac ggtgatccgg
     1441 ctcgcgcctc cagcaccgtt cgttccggaa gaatagcacg gcaagccagt cggcggcatg
     1501 ttggtctgct acagcgggga cgcacccgac tcagcgctcg ctcccatcta cggcatcggc
     1561 gatccgattg cgaacgtagt cacctccaag ccgtacgttg cacagcagtc gatgctggat
     1621 gcaaacgagc cgaagggacc gcaccgctac tggaagaccg agtatattcc ggagctgccg
     1681 gacgggtttc tgagcgcctt tcgtgagggt gggctcgcag tcacctctcc gctgttccaa
     1741 ggggtcatat ttcacctcgg gggagcacta aacgaacgca gcgcggacga cggggccgcc
     1801 gacaacagag acgcctaatt cattagcggc ttctcgggta cgtggccgcc cgacgcacct
     1861 gcggaaccgc atgtcaaatg ggtccgggat tcctgggaga gtatccgcgg ctactcgacc
     1921 ggcggcaact acgtgaactt ccagctcgcc gacgacgacc agaatcgact gcaggccggt
     1981 tatcgggata acttccagcg gctccaagac atcaaagccg aatacgaccc ccacaacctg
     2041 ttccgggtca atcgcaacat aagcccggcc gcgtagagcg ggccggctac ccggtccgct
     2101 ctttctgctt ggatgagcag attggggccg ggagccggtg atgcctgcga agagggcggt
     2161 gaatcgagga ccgttccggt tcacactctt gaggttcaca cttttaaagc tgcataattt
     2221 acatgtagag tggggctata cgctcttgcg ctgcagcgcg tggtcttggc tctgccggta
     2281 gcctggttcg aggggaggtg ggtgcgggcg agatgagaga attgtgggaa ttcttctctt
     2341 caaaccggga agcagtgctg gatttgacgg tggagcatat atggctcgtc ctggcggccg
     2401 tctcgattgc agttgtgctg gggatcgtct tgggtctact gtcctcctac tgggtgtctt
     2461 ccccggtgcg ctggtctaac ctcttctgga gctacgcact gtcgcgcgct cccgcttccc
     2521 tggtggcgag ctttctctac gtgccatccg ccttgaccat cttgatcgcc tgggtgtggt
     2581 tgcgtgaggt gcccacggcg ctcacggtgg tcggggggtc ggtcgcggtg ctgagggtgt
     2641 tcatcgtgaa cacttggggg cggcggaggc ttccgcaggc atcctagacc tccgggctgc
     2701 agcccgcgcc gacttgagtg attcagtacg gcgtttcctg ggaaaaagag aatacagact
     2761 agaacaatgc gtgaggtccc gccaggtgtg cggggaagag catcagctgt tatgtaatct
     2821 gaatccccac cgtgccctac tcgctgccct ccgcgcccgg accgtctttg atgaactcgt
     2881 agcccacttt ctcacaggcg acgaagccac cgtcgaggtt gtagaggttg gtgtagccct
     2941 cctctgccca cacggcggcg gcgattcggc tcatgtggtc gctctgacaa tagacgacga
     3001 ttttcgcgtc cttgtcatcg ggcagctggt ccacgtgccc ggcggcctcg tcatagggga
     3061 tgaagagatc cgttagcgga atctccccct catagg
//
LOCUS       DASRMJ010000148         4453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_372722, whole genome shotgun sequence.
ACCESSION   DASRMJ010000148 DASRMJ010000000
VERSION     DASRMJ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372722"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFD74_04095"
     CDS             complement(<1..198)
                     /locus_tag="VFD74_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48763.1"
                     /translation="MRSAAVAYPLEDLVGWERSLIAFLVEKERRSGSRRTVEGYSRML
                     QDFFGRLSKTPDEVTAQDVFVW"
     gene            complement(371..1060)
                     /locus_tag="VFD74_04100"
     CDS             complement(371..1060)
                     /locus_tag="VFD74_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48764.1"
                     /translation="MDTDLPPAAPADHLQHALATLASFASPEELTLRISLLESSLASL
                     TREQAADRLVEENVDSVVLDAALTIKQLAGRINDIVHAVGILTALPYVLEPGEVILGL
                     SLASGNTGKAYDLETDRQIAEFKFVTWQGGPESIRQNGLFIDLFRLASADTDKRRLLY
                     VVGERQPLRFLNGRRSLKSALSRNASAALSFQKLHGSRFSTVGEYYATVRDRVKIVDL
                     ADIVPELRLPE"
     gene            complement(1134..1319)
                     /locus_tag="VFD74_04105"
     CDS             complement(1134..1319)
                     /locus_tag="VFD74_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZK48765.1"
                     /translation="MDFVGIVGAVLGGFLMNQFGQSGVTGWNWPSFGVAVLGAVVLLV
                     VVRMVRGGGGGFRGRGR"
     gene            complement(1488..2813)
                     /locus_tag="VFD74_04110"
     CDS             complement(1488..2813)
                     /locus_tag="VFD74_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HZK48766.1"
                     /translation="MKFLVLKYWGNLRSSFWFLPSAMAAGAAALAFGAIAFDRAVLTE
                     RFGNVGWAYAGGAEGASAVLQTIAGSMITIAGVVFSLTLIALSLASSQLGPRVLRNFM
                     RDTTNQLVLGTFVATFLYCLLVLRSIVRTGDVFVPHLSVTIGVGFALASLGVLIFFIH
                     HVSVSIQADRMVARIGGELLRAVDRLYPDALSPDDAPPGKGRPDAAVPDEFDQEARVI
                     GSPDDGYVLLIDTDALTRLAEEEEAILRLERRVGEYVVSGAPLVSIWPGSRLTGDVED
                     RAGSAFVVGSQRAPGQDFEFTVIQLVEIAVRALSPGVNDPFTAIAAVDRLGSALCRLA
                     QRGSPPAAQFDDEHHLRVVGRPLTFPEVIDTAFNQIREYGRSSTAVTMRLLETLATIA
                     GFARRPEDRAAIRRHADMIARGARDGLPEVEDHRAVGIQRDTVMRILEQ"
     gene            3231..3551
                     /locus_tag="VFD74_04115"
                     /pseudo
     CDS             3231..3551
                     /locus_tag="VFD74_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            4101..>4453
                     /locus_tag="VFD74_04120"
     CDS             4101..>4453
                     /locus_tag="VFD74_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007544775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48767.1"
                     /translation="MLDGVASSVECAYKPVRWALRLRYLVRPAALVVGSALVYKRWLR
                     HWSFSWGASPEEEFESMPGDDFVTDPQWITTRAVSIDAPPSRVWPWLVQMGQGRGGLY
                     SYDRLENLVGSRVSS"
BASE COUNT          854 a         1366 c         1489 g          744 t
ORIGIN      
        1 ccatacgaag acgtcctgag cggtgacctc atccggggtc ttgctcaacc ttccgaagaa
       61 gtcctggagc atgcgggagt agccctccac ggtgcgacga gaaccggagc gacgctcctt
      121 ctcgaccagg aaggcaatga gggagcgttc ccagccgacg agatcttcga ggggataggc
      181 gacggcggcg ctgcgcatgg ggtcctcctt ggtcagattg acaccatgtt tgcgttcgtt
      241 cggagacaca gcaagtcgtc gagggagcgg cagtggagcg gccgggaggc aagggtccgt
      301 gcctgcattg ggcggagaag cgcactctct ggatgggtcg cgcgtggggc gtcaggcatc
      361 gatgctcgga ctactccggt agacgaagct ccgggacgat gtcggcgagg tcgacaatct
      421 tgacgcggtc ccggacggtg gcgtagtact cacccacagt cgagaagcgt gagccgtgaa
      481 gcttctggaa actgagtgcc gcagatgcat tgcgactgag ggcgctcttc agagaccggc
      541 gtccgttgag aaaccggagt ggctgccgct cacctacgac gtagaggagg cgacgtttgt
      601 ccgtgtcggc gctcgccagc ctgaagaggt cgatgaagag cccgttttgg cggatggatt
      661 ccgggccgcc ctgccaggtg acgaacttga actccgcaat ctgtcggtcg gtttcgaggt
      721 cgtaggcctt gcccgtgttg ccggaggcaa gcgagaggcc caggatgacc tcaccgggct
      781 cgagaacgta gggcagggcg gtgaggatgc caacagcgtg cacgatgtca ttgatacgac
      841 ctgcgagttg cttgatggtg agggcagcat ccaagacgac ggagtcgacg ttctcttcga
      901 caagccgatc agctgcctgt tcgcgggtca gggaggctaa actggactcg agtagtgaga
      961 tgcggagcgt gagctcctcc ggagacgcga acgaggccag cgtggccagg gcgtgttgca
     1021 gatgatcggc gggagccgct ggtggcaggt cggtgtccat cagagagagt ctagcgtaga
     1081 cgccgcccct ctgggccgcc cgcagccggg tggccgccga gatcgaggag cgctcagcgc
     1141 ccccgccccc ggaatcctcc tccgcctcct cgtaccatcc gcacgacgac caggagcact
     1201 accgctccca ggacggccac cccaaaactc ggccagttcc atccggtcac gccagattgc
     1261 ccgaattggt tcatcaggaa tccgcccaac acggctccca cgatgcccac aaaatccacg
     1321 ccaggatact catggtcgaa ccccctattg agatcgtgtt gccggtcgag tctctgcgct
     1381 tgatggtgtc ccgtcaagtg agcgcatatg ttgtcaaggt ctcccgagcc gacacgcgca
     1441 gctccttgtg tcactcggca aaatggcgct tgaggcaccg tccatcgtca ctgttcgagg
     1501 atccgcatca ccgtgtcgcg ctggatcccc accgcccgat gatcctcgac ctctggcagc
     1561 ccatcacggg ctccacgggc gatcatgtcc gcgtggcgac gtatcgccgc ccggtcttcc
     1621 ggtcggcggg caaaccccgc gatcgtggcc agggtctcca acagccgcat tgtgacagcc
     1681 gtgcttgagc ggccatactc cctgatctgg ttgaaggccg tgtctatgac ttccgggaag
     1741 gtgagggggc ggccgaccac gcgtagatgg tgctcatcat cgaactgcgc cgcgggcggg
     1801 gatcccctct gtgcgagccg acagagagcg gatcccaatc ggtcaacggc cgctatcgcc
     1861 gtgaaggggt cgttcactcc cggggagagc gcccgcaccg cgatctcgac gagctggatc
     1921 accgtgaact cgaagtcttg cccgggggcc cgctggctgc caacgacgaa cgcagaccca
     1981 gccctgtcct ccacgtcccc ggtcaggcgg ctgcccggcc agatgctcac cagtggcgca
     2041 ccggagacga cgtactcgcc gacccgccgt tccaggcgta ggatggcctc ctcctcttcg
     2101 gcgagcctcg tcagcgcatc ggtgtcgatc aggaggacat agccgtcatc cggactgccg
     2161 atcacgcggg cttcctgatc gaactcgtcg ggcacggcgg catcgggacg ccctttgccg
     2221 ggcggagcgt cgtccgggga cagggcgtcc ggatacagac ggtcgacggc ccgcagcaac
     2281 tcgccgccga tccgcgccac catccggtcg gcctggattg aaaccgagac gtggtggatg
     2341 aagaagatca acacccccaa gcttgcgagc gcaaaaccta cgccgatcgt gaccgaaagg
     2401 tgcggtacga agacgtcacc ggttcggacg atggatcgca ggacgaggag gcagtagagg
     2461 aaggttgcga cgaaagtgcc caggacgagc tggttggtgg tgtcccgcat gaaattgcgc
     2521 aggacccgag gaccgagctg agatgaggcg agcgacaatg cgatcaaggt caaagagaat
     2581 acgacgcccg cgatcgtgat catcgagccg gctatggtct ggagtaccgc gctggcccct
     2641 tcggcaccgc cggcgtaggc ccagcccacg ttcccgaacc gctcggtgag caccgccctg
     2701 tcgaaggcta tcgctccgaa cgccagagcg gctgcacccg cggccatggc tgacggcagg
     2761 aaccagaagc tggaccggag gttaccccag tacttgagaa cgaggaactt catgccctag
     2821 gacctcccgg actccgattc cccgaccttc cgagacctgc ggcgctcctc ttcccagcgc
     2881 gatcgcggtt cacgcctcga taggggctcg gatcgtaacc cccagcaggg gaggtcttcg
     2941 tctcaggcct caatccgggg tcgcggcggc tccgtcgtcc ccacgcgcgt gggggtggac
     3001 cgggtatcgt gacacggggc tgcggatcct gatgaggaaa gaaggactgt ttgtcctacc
     3061 ggatccacgt atccaccgag ccgactccag ggctggttcg acgctaaagc cctctggccc
     3121 cgcaagcact actccccgga agaggaggta ttcgccgagg ttctcctcct ggttcagcga
     3181 ctgcgagagc aggtatccag tctcaatctc caacggcagt tctggcggag cggtacctct
     3241 ccatccgcta caccgagcgc ctcacggaag ccggcgccgt cacctcggtg ggatcacgcg
     3301 gcgacagcta cgataacgct ctggccgagt cgatcatcag gctctacaag acggagctca
     3361 tccgcaagcg tgggccctgg cgaacgctcg aagacctgga attcgctacc ctcgagtggg
     3421 tcgactggtt caaccaccgt cggctgttgg agcccatagg cagcatcccc gcggatgagt
     3481 tcgaggagat gtgcgcccat cagctagccc actccgagga agagggactc aaacatacga
     3541 gtctccggtg agcccgcggt tcagtatcgg agggacgcca atcagactcg atgtatcggc
     3601 ccggtgctcg tcgggtagtg ataggaaaga tgggagggac actcgtgcaa cgtgatggga
     3661 ggcgggcgat gagaggccga aaacgcagcg gctttcgggg cgcggcccaa aagctctccg
     3721 agcgagcggt agctgggctt tcgaagctcg atgcggaaga taggctcgat gtcctgagcc
     3781 aagcggctga agacttggtt gacagggcgg aagaagcgat ccccagagtg cggaagaagg
     3841 ccaagcgggc gaccggtcgc gccgcacaat tggcggaccg tggggtgagg gaagcggccc
     3901 gctatgcacc ggaagcgcag aaaaaggtgg gggtgctggc atccgaggcg cagaagaagg
     3961 cggcaaccct cgcctctgat gccggagccc gagcagaggg gtacgccgct gagggagcgc
     4021 ggcttgcgca gcactaccgt ggcaaggcgg cgcgtgaggc tcaggagaag tctgccggcg
     4081 tcgcagcaag gacggggtca atgttagacg gggtggcctc gtcagtggag tgcgcctaca
     4141 aacccgtgcg ctgggcgttg cgccttcgat acctggtccg tccggcagcc cttgtggtcg
     4201 ggtcggcact ggtgtacaag cgctggctga gacattggtc gttctcttgg ggtgcttccc
     4261 cggaggagga gttcgaatcc atgcccggtg acgacttcgt gaccgatccg caatggatca
     4321 cgacgcgggc agtgagtatt gacgcaccgc cctcgcgggt ctggccctgg cttgtgcaga
     4381 tgggccaagg ccgcggcgga ctatacagct acgaccgcct cgagaatctc gtcggaagcc
     4441 gagtgagcag tga
//
LOCUS       DASRMJ010000149         8912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_484262, whole genome shotgun sequence.
ACCESSION   DASRMJ010000149 DASRMJ010000000
VERSION     DASRMJ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8912
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484262"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFD74_04125"
     CDS             <1..696
                     /locus_tag="VFD74_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZK48768.1"
                     /translation="RSRLDDPSLSLESRRAVELMVGDVGRLRRLIEDLLEISRMDSGA
                     EELRVEEFDVAAFLQQVSQARGWPRQVELRIDPAESGIPVATDRRRLERIVVNLVENG
                     LRHGAPPIWVDARVVHDPGGGESQFALDVTDHGAGIPPEHLSEVFQRFYKADPSRTSS
                     GGAGSGLGLAIAWENARLLGGLLRVKSNVGEGTRFSLRIPVTQPLRQRETPVIPGAQN
                     DSTGFLQEEADNE"
     gene            689..1672
                     /locus_tag="VFD74_04130"
     CDS             689..1672
                     /locus_tag="VFD74_04130"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZK48769.1"
                     /translation="MSDDAYTQEKRHVQGGGRRSAPLPSLLTTSTAILVVMSAALLAL
                     AGCGYSSTVKDGGPVTTVAGTGTTESTGPTTDTTTPEQTTTTVPGETVDVLAFFMKGE
                     YLAPVRRHVPATKEVAKAAMMELVAGPTAEEQAAGFVTAIPDKTLFLGVEMQGSVATV
                     DLSKEYESGGGSMSMFARLGQVVYTLTQFPSVSSVSFKLDGQPIEVFSGEGLLLEHPM
                     TRADNEYVTNPIHVESPVFGDTVTSPLRIYGTSNVFEATSQVRILDSAGGTLNEVYVT
                     ATSGTGTRGTYDVTVPFTAAPGSDITLMSFEYSAKDGSMINISEIPLHVAP"
     gene            complement(1847..4624)
                     /locus_tag="VFD74_04135"
     CDS             complement(1847..4624)
                     /locus_tag="VFD74_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZK48770.1"
                     /translation="MVPGSSSPKLSVPQQGQVLPRHRLRTAARLLRGGGVITLAAGPG
                     YGKTAAMADMLSLAAPDSAYVALDEDDRDPLRLLTFLIEALDAPCPGLGREALAVLHQ
                     DSTQTGAVSEVEAILAEELRAYMGPNVVVGLDDVHKVASSSAAVNVIGWLARFLPNGW
                     VLVLSSRGPITLDLDPLRDAGRCIDIGTRRLRLTPTEVKEWTREFWGVDLSLADARAV
                     WRMSEGWPVALVLMGRHLSRDGRLPTRERVLALARHGRHLNQYLAQGVLSTLDPDTAT
                     FLMEAFPLERVVFPRDAAVFSVSCARAERLCEEMADAGFLMMRTGHRAYTLHRLIRDF
                     AETQARRAGVEKAESTMCGAAAHLEGIGEHRAAIDLYLRAGRPEGAITSVRHILAGNL
                     NASQAIARHEWLELLPDASIGHEPWLALLKARILQDRGGWREAEPLYAVAHRSFAQLH
                     DQDGVFQAALGQGFCLYVLGRWDDCLSALMRAEKAAARPAEKTEVGVNISAVLLALCR
                     WDDAVERLELVLAACPPVARKALEIRVAGHRARLFMLRGRYLTALDWARRAVRMSHSH
                     TVLHTATALNTAATTLYLTGRYEEARIQVDAARALIASRGLAFLEVPSRLTVAAVCMG
                     EGDFRAAVQHLREAVELGTETGDMEALVWAEDLLGQICRCNRGAERALEHHRTALDLV
                     EQHQLSMSDRVKILCGVGMDLAVSGAEDEAGAVLAQVIQLSRRWGFEAPLSHALFYSG
                     WFSAQRGDEAGAGRALGEAMRLAAANGFVHFYHLESRVATPILALCSRIGSGAFVEDH
                     VVPTLPERHQRYYRRLAHGDTYPTDVRIGRLPSRLSAAGRDSLPVTGEERELARRFDN
                     LTARESEILGLIAEGLPNKLVATRLYITEKTIKTHTNNIYRKLGVQNRLQAVLAHQEF
                     ERLAARGGSHG"
     gene            complement(4795..5082)
                     /locus_tag="VFD74_04140"
     CDS             complement(4795..5082)
                     /locus_tag="VFD74_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZK48771.1"
                     /translation="MNSVQLVGRLATEIDLKEVTGGSKVSSFILAVDRNAEEADFFRV
                     KVWNSQAEAAADNLAKGRRVGVEGSLRQDTYEKDGEERKAVSVVARRLEYL"
     gene            5376..5522
                     /locus_tag="VFD74_04145"
     CDS             5376..5522
                     /locus_tag="VFD74_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48772.1"
                     /translation="MDANLPTGLVALTLSALLAEVLREQSADRDFPALLVANMALLPF
                     VRPK"
     gene            5845..6990
                     /locus_tag="VFD74_04150"
     CDS             5845..6990
                     /locus_tag="VFD74_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012580122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HZK48773.1"
                     /translation="MTKVTAKKTSYVSRLSQVVNVPAEERAALEVVAARYAFRANTYY
                     LGLIDWDDPEDPIRRIIIPSAEELGDWGRLDTSNEHCYTKVPGCEHKYEFTGLLLVND
                     LCGGYCRFCFRKRLFMHGNTEVQRDVGPGIEYIRQHPEINNVLLTGGDSLLLSTKHID
                     HILSELRAIEHVQIIRFGSKMPAFNPFRITDDPSLLEVLARHSLDDKKIYVMTHFNHP
                     RELTPEAVKAVNLLQKAGVVTANQTPLLRGVNDDPEVLGELFNRLSYAGVPPYYVFVG
                     RPTRGNRHFAVPVEEAFEIFERARMKCSGLAMRARLVMSHATGKIEIVGRTETETYFR
                     YHRAADPTDKARFLVFPGNPEAYWFDDYVGALDDYAPTNPFACYELN"
     gene            complement(7030..>8912)
                     /locus_tag="VFD74_04155"
     CDS             complement(7030..>8912)
                     /locus_tag="VFD74_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZK48774.1"
                     /translation="FIWAIEVFHRLFFEQGGSAFGFLGGGYVILLPIIGGLIVGPLVH
                     FLAPEAKGHGVPEVMTAMAVRGGRIRPVVILVKALGSAVTIGSGGSVGREGPIVQIGA
                     AIGSVLGQRLKLSNTRILALVGSGAAAGVAATFNAPIAGVMFALEVLLGEYTVPAVST
                     MVFSAVSASVISRLFLGDHPSLAVPAYSLVSSWELLMYLGLGVVAAFGAGFFVRTLYF
                     AEDLFDDLRFPPYLKPVLGGALLGLLGYFLPQVFGTGFGVVEQTLNGSLALWLLVLLI
                     FAKVLATSLTLGSGFSGGVFAPALFVGAVLGGAYGKVMHGLFPAITATSGAYATVGMA
                     AVFAAAARAPITSIVILFELTLDYRIMLPLMFATVVATVVAARLEPESIYTLKLVRRG
                     IDFRARAGLGVTSGVDVGEVMTPMTTMHSAQPTMSLDDLAVLFHETGHHGVPVVDTDG
                     RFVGVVTLGDLERGLRSVGVEGTVADIMTVDLEVAYPDESLESVLRRSGAFDVGRIPV
                     VDRDDPSKIMGVLRRGDIIRAYSEALYKREDWFHRLDRRRVEHATGSHVVEFDLTADD
                     AAVGHALRDLNLPPECLIISIHRKGARIVPRGDTRLEAGDHLVAVAPYDGGRRLGRCL
                     HG"
BASE COUNT         1668 a         3050 c         2788 g         1406 t
ORIGIN      
        1 cggtcccgtc tggacgatcc gagcctctcg ctcgaatcgc ggcgcgccgt ggagctcatg
       61 gtcggggacg tgggtcgcct aaggcgtttg atcgaagacc tgctcgagat cagccgtatg
      121 gactcggggg ccgaggaact cagggtggag gagttcgacg tcgccgcgtt cctccagcag
      181 gtgtcccagg cgagagggtg gccgcgccag gtggaacttc ggatcgatcc cgccgaatcc
      241 ggcatcccgg tcgccaccga ccgcaggcgc ttggagcgga tcgtggtcaa cctcgtcgag
      301 aacgggctac ggcacggcgc tcctccgata tgggtcgacg cccgcgttgt tcacgacccc
      361 ggtgggggag agagccagtt cgcgctcgac gtcaccgatc atggagccgg catcccccca
      421 gaacacctaa gcgaggtatt ccaacgcttc tacaaggccg accccagccg cacctcgagt
      481 ggcggcgcag gaagcggcct cggactggcg atcgcctggg agaacgcccg actcctgggc
      541 ggactgctgc gggtcaagag caacgtgggc gagggcactc gcttctctct gcgcatccct
      601 gttacgcaac cgttacgcca aagagaaaca cctgtgattc caggggcgca gaatgacagc
      661 actgggttcc tgcaagagga ggcggacaat gagtgatgac gcgtacaccc aagagaagcg
      721 gcatgtacaa ggaggcggtc ggcggtcggc gccactgcct tcgctcctga cgacgtcgac
      781 ggcgatcctg gtggtgatgt cggccgccct gctggcgctc gcaggatgcg ggtactcatc
      841 gaccgtcaag gacggcggac cggtgaccac cgttgccggc accggcacga ccgagtcgac
      901 tgggcccacc accgatacca ccacccccga gcagaccacc acgaccgtac cgggcgagac
      961 ggtcgatgtg ctcgcgttct tcatgaaggg cgagtatctg gcgccggtcc gccgtcacgt
     1021 tccggcgacc aaagaggtgg ccaaggcggc catgatggaa ctggttgccg gacccaccgc
     1081 cgaagagcag gccgccggct tcgtcacggc catccctgat aagacgctgt tccttggggt
     1141 cgagatgcag ggctcggtcg ctacggtcga cctctccaag gagtacgaat cgggcggcgg
     1201 cagcatgtcc atgttcgccc gcctcggaca ggtcgtgtat acgctgacac agttcccgtc
     1261 cgtcagcagt gtgagcttca agttggacgg acagccgatc gaagtgttca gcggcgaggg
     1321 cctgctcctc gaacatccta tgacccgagc tgacaacgag tatgtgacga accccatcca
     1381 cgtcgagtca ccggtgttcg gagacaccgt gacgagtccg ctccgcatct acggcacgag
     1441 caacgtgttt gaagccacgt cgcaggtgcg catcctggat tccgccggcg gcaccctgaa
     1501 cgaagtgtac gtcaccgcca cctccggcac cgggacccgc ggcacctacg acgtcaccgt
     1561 gcccttcacg gccgccccgg gaagtgacat cacgctgatg agcttcgagt actcggccaa
     1621 ggacggctcg atgatcaaca tcagcgagat tcccctgcac gttgccccat gaatcggacg
     1681 ggcgcctgag ctggatgggc gcctgagccg gacgggcgac cggccggcgc cccgaacgcc
     1741 cagaggccgg agagctgatc tccggcctct tcttcttccc cgcgccgggc acgcagcacg
     1801 cagtctccgc gggcccatcg acggcgccgg gttccctcga ccgccgtcac ccatgcgatc
     1861 cgcctctggc ggccaatctc tcgaattcct gatgcgcgag cactgcctgc agtcggttct
     1921 gcactcccag cttccggtag atgttgttgg tgtgggtctt gatggtcttt tcagtgatgt
     1981 agaggcgcgt ggccaccaac ttgttcggaa gcccctcagc gatcagcccc aagatctccg
     2041 actcccgagc ggtgagattg tcgaagcgac gcgcgagttc tctctcctcg ccggtgacgg
     2101 ggaggctgtc gcgccccgcc gccgacagcc gggacggaag ccgcccgatc cgcacgtcgg
     2161 tcgggtacgt gtctccgtgg gccagccgtc ggtagtagcg ctgatggcgt tcgggcaggg
     2221 tgggaacgac gtggtcctcg acgaaggccc ccgatccgat gcgagagcag agtgccaaga
     2281 tgggcgtggc aacccgtgac tccagatggt agaagtgcac gaagccgtta gcggcggcca
     2341 ggcgcatcgc ttcgcccagt gcgcgcccgg cgccggcctc gtccccgcgt tgggcggaga
     2401 accaccccga gtagaacagg gcgtgggaga ggggcgcctc gaacccccag cgccgtgaca
     2461 gctggatcac ctgggcaaga acagcgccgg cttcgtcctc cgcgcccgaa accgccaagt
     2521 ccatgcccac gccgcacagg atcttcactc ggtctgacat cgagagttgg tgttgctcaa
     2581 cgagatcaag agctgtccga tggtgctcca aggcgcgttc ggcgcctcga ttgcaccgac
     2641 agatctgccc gagaaggtcc tctgcccaca ccagggcttc catgtcgccc gtctcggtgc
     2701 ccaactccac cgcctcgcgc aggtgctgca cggccgcccg gaaatcgccc tcccccatac
     2761 agacggcggc cacggtgagc cgcgaaggaa cctcaaggaa ggccaagcct cgggatgcga
     2821 tcagcgcgcg ggccgcgtct acctggatgc gtgcttcctc gtacctaccc gtgaggtaca
     2881 gggtggtggc tgccgtgttc agcgcagtgg ctgtgtggag cacagtgtgc gagtgggaca
     2941 tccgtacggc tcgcctggcc caatctaggg ctgtgaggta gcgcccgcgc agcatgaaga
     3001 gccgagctcg atggcccgcg accctgatct ctagcgcctt ccgcgcgaca ggcggacagg
     3061 cggccagaac caactccaga cgctcgaccg cgtcgtccca ccggcacaag gccaggagga
     3121 cggcactgat gttcacccct acctcggtct tctccgcagg cctggcggca gccttctccg
     3181 cccgcatgag agcgctcaga caatcgtccc aacgccccaa gacatagagg cagaatccct
     3241 ggccgagagc ggcttggaag acgccgtctt ggtcgtggag ttgcgcgaag ctacgatggg
     3301 ccacggcata aagcggctcc gcttcacgcc atccaccgcg gtcttggagg atccgcgctt
     3361 tcagaagagc cagccacggc tcatggccta tgctcgcatc cggcagaagc tccagccact
     3421 cgtggcgggc gatggcctgg gaagcgttca gattcccagc caggatgtga cgcacggagg
     3481 tgatggcccc ctcgggccgt ccggcccgca gatacagatc gatggcagct cgatgctctc
     3541 cgatcccctc caggtgcgcc gccgccccgc acatggtgct ctcagccttc tcgacgcccg
     3601 ctcttcgggc ttgggtctcg gcgaaatctc ggatgagacg atgcaacgtg tacgcccggt
     3661 gacccgtcct catcatgagg aacccggcgt cggccatctc ttcgcacaac cgctcggctc
     3721 tcgcacacga cacgctgaag acggcggcgt cccgggggaa gaccacacgc tccaggggga
     3781 aggcctccat caggaaggtc gcagtgtccg gatccaaggt ggagagcacc ccctgcgcga
     3841 ggtactggtt gaggtgtcgt ccgtgccgag cgagcgcgag cacgcgctcc cgggtgggca
     3901 gcctcccgtc tcggctcagg tgccgcccca tgagcacgag cgccacgggc cacccctcgg
     3961 acatccgcca caccgccctg gcatccgcga gggacagatc gacaccccag aactcgcggg
     4021 tccactcttt gacttcggtg ggggtcaggc gcaaccgccg ggtccctatg tcgatgcagc
     4081 ggccggcgtc ccgcagaggg tcgagatcca acgtgatcgg gccccgggac gacaagacca
     4141 atacccaccc gttcggcaga aaccgggcca gccacccgat gacgttgaca gccgcagatg
     4201 aggacgccac cttgtggacg tcgtccagcc cgacgacgac gttcgggccc atgtaggccc
     4261 gcagctcctc ggccagtatc gcctcgacct ccgagaccgc cccggtctgc gtggagtctt
     4321 gatgcagcac cgcgagagcc tcgcggccga gacccgggca gggtgcgtcg agtgcttcga
     4381 tcaggaacgt gaggaggcgg agcgggtcgc gatcatcctc gtcgagggcg acgtacgcgg
     4441 agtccggagc cgcgagagac agcatgtcgg ccatcgccgc cgtcttgccg tatccggggc
     4501 ccgcggccaa agtgatgacg ccgcccccgc gcagcagtcg cgccgcggtc cgcagtcgat
     4561 ggcgcggaag gacctgcccc tgctggggca cactcagctt cgggctcgac gacccaggca
     4621 ccatccacac ttccccatcg gaagaggtga caggtccgag cgcaccgaca ctcccgcgcg
     4681 gtatcgctgc cgcctgtcct gctatctgtg caaaggtaac catcttcact cgctctggct
     4741 agagggaagt gcgggccggc gcgccggccc gcaccagatc acgtgtgcga tcgatcagag
     4801 atactcgagg cgtcgggcga ccaccgacac ggccttgcgc tcctcgccgt ccttctcgta
     4861 ggtgtcctgc cgaagactac cttccacgcc cacgcgcctc cctttggcca ggttgtcggc
     4921 ggcggcctcc gcctgcgagt tccacacctt gacgcgaaag aagtccgcct cctcagcatt
     4981 ccggtccacc gccaagatga agctggacac cttcgacccg ccggtcacct ccttcagatc
     5041 gatctctgta gccaaccgac ccacgagttg cacgctgttc ataatcccct ccttgtagac
     5101 gcaacctgcc tcaccaccgc cgccggatcc gacccgcctc gcgggtgggc cgcccggccg
     5161 caccccttgg tagatcgagg gcgaccgtga agacaaggta gcgggcgcgc gtctgcttcc
     5221 gaatactcca gtggttggaa ccagagtgac gagagggccc gaggccattt gcgcctgtgg
     5281 aaaaccacgg tccccgtagc catgccgcag tcccgtaaga gggggtgtga aggggaatta
     5341 agtcttagcg tggatttgac gataagtaga acacgatgga cgccaacctc cccacaggac
     5401 tcgtagcgct caccctgagc gccctgctcg ccgaagtgtt gcgagagcag tccgccgacc
     5461 gggacttccc agcgctcctc gtggccaaca tggccctgct cccgttcgtg agaccgaagt
     5521 agcctctgtc cctcgcgctg cggcccgcca cgacccggcg ccacctgact ctctgcggca
     5581 taccgacacc cggcccggat ccgcgaccgg cttcattcac cgtattcccc tgtagttcgt
     5641 gacattcgcc accaaagctc ttgagccacc agggtttctt gagttatact gctgatccga
     5701 ttacgtgatc ggacttgcgt ccttgacgca gcaacgaccg ccccgatggc ggcaacggtg
     5761 ctcacgcacc cttggcccga cggccccccg accagcgacc aacgcccttt cgccaccccg
     5821 cgaaggcgcg caccggaggt gcacatgacc aaggtcaccg cgaagaagac ctcgtacgtg
     5881 tcccggttgt cccaagtagt caacgtgccc gcggaggagc gcgccgcgct cgaggtcgta
     5941 gccgcgcgct acgcgttccg ggcgaacacc tactatctcg gtctgatcga ttgggacgat
     6001 ccagaggacc ctatcagacg catcatcatc ccaagcgccg aagagttggg ggattggggt
     6061 cggctcgaca cctccaacga gcactgctac accaaggtcc cggggtgcga gcacaagtac
     6121 gagttcaccg gcctccttct ggtgaacgac ctgtgcgggg gctactgtcg gttctgcttc
     6181 cgcaaacgcc tgttcatgca cggcaacacc gaggtgcagc gcgacgtggg acccggtatc
     6241 gagtacatcc ggcagcatcc ggagatcaac aacgtgctgc tcacgggcgg tgactcgctt
     6301 ctgctctcga ccaagcacat cgaccacatc ctatcggaat tgcgggcgat cgagcacgtg
     6361 cagatcatcc gattcggcag caagatgccg gcattcaacc ccttccgcat caccgacgac
     6421 ccctcgctcc tcgaggtgct cgcgcgtcac agcctggatg acaagaagat ctacgtcatg
     6481 acccacttca accacccgcg cgaactcacg cccgaagcgg tcaaggcggt gaacctcttg
     6541 cagaaggcgg gcgtggtcac tgccaaccag actccgctcc tgcgaggcgt caacgatgat
     6601 cctgaggttc tgggcgaact cttcaaccga ctgtcatacg ccggcgtgcc gccttattac
     6661 gtgttcgtcg ggcgcccgac gagaggtaac cgtcacttcg cagtccccgt cgaagaagcc
     6721 tttgagatct tcgaacgggc acgcatgaag tgctccggac tggcaatgag ggcgcgtctc
     6781 gtgatgtcgc acgccacggg caagatcgag atcgtcgggc gcacagagac ggagacctac
     6841 ttccggtatc accgagcggc ggaccccacc gacaaggcac gcttcttggt ctttcccggc
     6901 aaccccgagg cctactggtt cgacgactac gtcggggcgc tcgacgacta cgcgcccacc
     6961 aacccgttcg cctgctacga gctgaactga gagaattcag gcccgaccgc gattgcgcgc
     7021 cctgaaggcc tagccgtgta gacagcgccc aagtcggcgg ccgccgtcgt acggagcgac
     7081 cgcgacgaga tggtcaccgg cctccaaacg ggtgtcgcca cgcgggacga tgcgtgctcc
     7141 cttgcggtgg atcgagatga tcaggcactc cggcggcaga ttgagatcgc gcagagcgtg
     7201 accgaccgcc gcatcatctg cggtgagatc gaactcgacc acgtgggaac ctgtggcatg
     7261 ctcgacccgg cggcggtcga gccggtggaa ccaatcctca cgcttgtaca gcgcttcgct
     7321 gtacgcacgg atgatgtcgc cgcgccggag aacccccatg atcttgctgg ggtcgtctcg
     7381 atccaccacc gggatacgtc ccacgtcgaa cgctcccgag cgacgcaaga ccgactcaag
     7441 ggattcgtcc gggtaggcga cttccaggtc gaccgtcatg atgtcggcga ccgtgccctc
     7501 gactccgacg ctccgcaaac cgcgttccag gtcaccgaga gtcactacgc ccacgaaccg
     7561 tccgtccgtg tccacgaccg ggaccccgtg gtggccggtc tcgtggaaca ggacggcaag
     7621 atcgtcgagc gacatcgtcg gttgggccga gtgcatggtt gtcatgggtg tcatgacctc
     7681 gcccacgtcc accccggagg tgaccccgag gcccgctctc gcccgaaagt ctatccctcg
     7741 cctcaccagc ttgagcgtgt agatgctctc cggctccagc ctggccgcta ctacggtggc
     7801 caccaccgtg gcgaacatga gcgggagcat gatgcggtag tccagggtga gctcgaagag
     7861 gatgacgatc gacgtgatgg gagcgcgggc ggcggccgcg aagaccgccg ccatgcccac
     7921 ggtggcgtag gcgccgctgg tggccgtgat ggcggggaag agaccgtgca tgaccttccc
     7981 gtatgcgccg cccaatacgg cgcccacgaa cagtgccggc gcgaagacac cgccggagaa
     8041 cccggagccg agcgtcagcg aggtggcaag gactttggcg aagatgagga gcaccaggag
     8101 ccaaagggcg agcgacccgt tcagggtctg ctcgaccact ccgaaacccg tcccgaagac
     8161 ctgcggcaag aagtagccga gcagtcccag caacgcacca ccgagcacgg gcttcaggta
     8221 ggggggaaag cgcagatcgt cgaacaggtc ttcggcgaag tacaacgtgc gcacgaagaa
     8281 cccggcgccg aaagcggcca ccacgcccag ccccaagtac atcagcaact cccaagagct
     8341 caccagcgag tatgccggca ccgcgagcga cgggtggtcc cccaggaaca ggcgactgat
     8401 gacgcttgcc gacactgcgg agaagaccat ggtgctcacc gccggcaccg tgtactcgcc
     8461 gagcaacacc tcaagcgcga acatcacccc ggcgatgggg gcgttgaacg tagcggccac
     8521 gccggccgcg gcaccggagc cgacgagggc gaggatccga gtgttgctga gcttcagccg
     8581 ctgacccaag accgaaccga tcgccgctcc gatctggacg atcggaccct cgcggccgac
     8641 ggacccccct gatccgatcg tgacggccga ccccaaagcc ttcaccaaga tcaccaccgg
     8701 gcggatgcgg ccgccgcgca cggccatggc ggtcatgact tcggggaccc cgtgaccctt
     8761 ggcctcgggc gcgaggaagt gcaccagcgg gcccacgatc agcccaccga tgataggtag
     8821 cagaatcacg tatccgccac ccaggaaccc gaatgccgag cccccctgct cgaagaacag
     8881 acggtggaag acctcgatgg cccagatgaa ga
//
LOCUS       DASRMJ010000150        17800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_128318, whole genome shotgun sequence.
ACCESSION   DASRMJ010000150 DASRMJ010000000
VERSION     DASRMJ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 17800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17800
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128318"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..745
                     /locus_tag="VFD74_04160"
     CDS             218..745
                     /locus_tag="VFD74_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48775.1"
                     /translation="MAKRVRKIYEDTADVAKEKVADATGAVKDRAIEASEVAKGKAVE
                     AIETIKDKAVDVLDTVVEKTRDLSEEARSVASDQLSRGSRQVASRVKATGESVHSVED
                     ALRRHGQEHAADVASHAADRLESVGSRLQDVGHRSRWSRFRAFLTKPWLLVAGALLVA
                     IVVYRFLKTPGERDT"
     gene            1236..1832
                     /locus_tag="VFD74_04165"
     CDS             1236..1832
                     /locus_tag="VFD74_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48776.1"
                     /translation="MVVHRSSLVILVIAIILLLGAVGLIGCGSTRSASTTLSPGTGAT
                     VPLPSGATTSLETTVSVGTTVLGGQTPALTFLGPVNLDTWRALREIVMNAGDDGQKVL
                     DEAAGLATGPPVEVGIAERDAVTGIGYAAGATTSQPVGVAIDVDGKQVVVFAFSVIGA
                     PDTTTIVGFERQTNLVGLVEGPLPISDSTGNMTTIPSP"
     gene            complement(2320..3177)
                     /locus_tag="VFD74_04170"
     CDS             complement(2320..3177)
                     /locus_tag="VFD74_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZK48777.1"
                     /translation="MRTGSWTSRLVRVVTRGPAAIFLLFVGVAWLIPAVGLLVSSFRT
                     GPANSSSGWWTALANPSQFTIENYRSLLQGSGMLHALWNTVLITVPVCTLVVVIAAMA
                     AYAFAWMRFPGRDALFLLVVALLVVPLQVALIPVADLYGRTGLFGTIPGVILFHTAFG
                     LPFAIFLLRNFFIGIPRDLLEAARMDGAGEFLVFTRVVLPLGTPAIASLLIFQFVWVW
                     NDLLVALVFARTSSAPLTVFVRDQLRQFGSNIDVIAPGAFLQMVIPLIVFFAFQRYFV
                     QGLLAGSVK"
     gene            complement(3180..4628)
                     /locus_tag="VFD74_04175"
     CDS             complement(3180..4628)
                     /locus_tag="VFD74_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZK48778.1"
                     /translation="MSDRQHPDGGSVAVPATDPPSDLSGALGASDLSGAVITSETRGP
                     RGPGRGRLASWFKGGQGSWKVAIFLLPGIFILGVLVVYPILFTLVRSFYSAHGDQFVG
                     ISNYQAMFTSADTLTSIKNNVIWVVVAPAIVAALGLVFAVLTERVPWATAFKVAVFVP
                     MAISFLSSGVIWRLVYQDDPRLGLANAALVGVSKVFQPPGPYPTARPSRPDVLVSDAQ
                     AYVTAADYRAGSTAQLGLVAVPPRLIPSDAVQAVAASTEAGSIGGTVWVDFAVGGTGT
                     SGVIDPGELGLPGVKVEVLSDAGDVIGSTKTGPDGTFAITGIEAGSYKVGLADSNFRP
                     AWGGILWLGPALITPSIIISYIWIWAGFAMVLIGAGLAAIPRDVLEAARVDGAGEWQV
                     FRRITGPLLAPLLLVVLVTLLINVLKIFDLILVIPPGSVLADANVIALEMWQVSFGGG
                     QDQGLGSALAILLFILVLPAMAFNLRRFRAEQ"
     gene            complement(4674..6035)
                     /locus_tag="VFD74_04180"
     CDS             complement(4674..6035)
                     /locus_tag="VFD74_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZK48779.1"
                     /translation="MRIGRTTIVLLLAALLLVVVGCSGGTTTTTTAAGGETTASAAPE
                     TSTTSAPDLSGQTMEVAAVWSGAEQDNFQKVIDAFQEQTGVKVTYTSTGDQIATILGT
                     RLQGGSPPDVAVLPQPGLMYSLADQGALQPLTDVIGMEMDANFAPVWKDLGTHEGTQY
                     GLVFKAANKSTFWYNMGVFSDAGVTPPTTWDELLAAADTIQRFGVPPFSVAGADGWTL
                     TDWFENVYLRTAGGEKYDQLTTLEIPWTDPSVTTALEQLAKIWGSDAMLSGGATGALQ
                     TDFPTSVVNAFTTPPKAAMVYEGDFVAGVIGSETQATLGTGADFFDFPSINGSPASVV
                     GGGDMAVLMKDSDAGKAFMKFLATPEAATIWAALGGFSSPNKNVDPSVYPDDITRRAA
                     TALATAEVVRFDMSDLEPPAFGGTPGKGEWKLLQDFLSNSSDISGIQKQLQDAAAAAA
                     SGS"
     gene            complement(6073..7281)
                     /gene="ugpC"
                     /locus_tag="VFD74_04185"
     CDS             complement(6073..7281)
                     /gene="ugpC"
                     /locus_tag="VFD74_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017582244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HZK48780.1"
                     /translation="MSEIYLDKLTKVYADGTLAVEDMDLEVQDGEFMVFVGPSGCGKT
                     TALRMVAGLEDITRGTVSIGGRVVNDMAPKDRDVAMVFQNYALYPHLSVYDNMAFSLK
                     MRKVDKAEIDKRVHEAARILGLDQLLGRKPRNLSGGQRQRVAMGRAIVREPQAFLMDE
                     PLSNLDAKLRVETRAQIARIQDDLNVTTIYVTHDQVEAMTLGDRVAVMKKGRLQQVDS
                     SQTLYDHPVNLFVAGFIGSPAMNMVEATVIREDGGLFVRFGGMRLRLDEKIESTRPDL
                     KKFEGRTIVLGIRPEDMHDAALQEAPAERRMRVIPDLIESLGSEALIHFTIDAPIVLT
                     EDVRELAVDVGAEKLERLEREREQGKSKFVAAVSPQTGAAVGQPMELAVDTARVHAFD
                     PETGLGVYGS"
     gene            7470..7613
                     /locus_tag="VFD74_04190"
     CDS             7470..7613
                     /locus_tag="VFD74_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48781.1"
                     /translation="MDEHIDRLTDAMEEQLGPFVQAVGLAGTIPGGDTSVVRRHGRRP
                     PRV"
     gene            7726..8904
                     /locus_tag="VFD74_04195"
     CDS             7726..8904
                     /locus_tag="VFD74_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZK48782.1"
                     /translation="MMNVLQAVGHALSIAGLMTWQILWALILGFALSAIIQALVRRET
                     VVGLLGDDRPRTLVVAAGVGAASSSCSYASVALARTLFMKGANFTAAMTFEIASTNLV
                     VELGIILALLLSWQFTLAEFVGGPLMIVMLAVLFRLLLRKRLLDGALAQAKKGLAGSM
                     EGHAAMDMSVRETGSIWKRLRSPSGFTSVSHVFVMEWAAVLRDIVGGLLIAGAIAAWV
                     PASFWRIFFLTEDPLLSKIWGPIVGPLVSVLSFVCSIGNVPLAAVLWTGGISFGGVIS
                     FIFADLIILPILLIYRKYYGTKMMLFLAGTFYVTMVAAGFLVEVIFGVLGLVPTERSS
                     QVVESHISLNYTSVLNVVFLALAGVLLWRFFRTGGREMLAMMSGAPGGMAGHEESTHE
                     "
     gene            8897..12127
                     /locus_tag="VFD74_04200"
     CDS             8897..12127
                     /locus_tag="VFD74_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HZK48783.1"
                     /translation="MSDGPAGGADAPEAKEAACLVVDSDRFDAVLFDMDGVLTTTARM
                     HAAAWKRTFDGFLDDWDAEHDSHTPRFSVAEDYPTSVDGKPRQEGVRDFLASRDISLP
                     AGTPDSPPTEWSVYGVGSRKQEFVEEALRAEGVEVFPGSVAWLRELREMGIGTAVVTS
                     SRNCDVILSAAGIADLFDAQVDGNTAIELGLTGKPAPDIFLEAARRLGAAPARAVVVE
                     DSQAGVEAGRAGGFGLVIGVDRADQADELRQAGADIVVADLAELVPCEPDVRHPAGPR
                     RHRLRASAIRLVAAEGDFPVDEHRLVERRFNPQYVPQLESIFALANGYLGLRGTQDEG
                     RPVYDPAPMLNGFYETWPLVYPERAHGFPDTGQTILGVPDGAILRLYVDDEAFSLEHA
                     DVLEYERVLDMGAGTLERRVVWRATDGSRFSVRSRRLVSFENRHLACIQYEVISLDRA
                     ADLTISSDLMIHPENGSLDSKDPRRNQALGPGVLQKTGEHVNDQRVVLAYQARASGLQ
                     MACGMDHRLTSGTAAAPPQAVVEGDWARVVYRIAAGQGEPVRLVKYLAYHYDGQEPAA
                     ELSFRVGQTLERAAAEGIDSVFAAQHAYVQDFWTRSDVITEGAPLLQQAVRFNLFQLL
                     QASARLEGHGMPAKGLTGRGYEGHYFWDAEIYVMPFLTYTAPHIARNLLLNRYAMLDK
                     ARRRALEVSQQGALFPWRTINGEEASAYYAAGTAQYHIDADIAYALTQYVRTTEDTEF
                     LARQGAEMLVETARMWVSLGFFSDRKDGRFVINGVTGPDEYSALVDNNTFTNLMAQEN
                     LRQAVRVVEWLGVSNPEAHAGLVERTQLDDAELELWQRAADLMYVPYDQELGLHLQDD
                     GFLDLEPWDFENTPLEDYPLLLHYHPLVLYRHQVIKQADVVLATFLLGDLFTAEDKRK
                     ILDYYDPLTTSDSSLSECIQCIMAAEVGDDLRAAEEYFIDAVGIDLGDVAGNVRDGVH
                     VASAGGTWMALVYGFGGMRDRNGELSFRPRLPGRMTRLSFKVHPRGATLEVDITAAGA
                     TYRLESEDELTIVHCGESLRLRSDIPVTRPLVTRVQEDDPTSVSRP"
     gene            complement(12157..13500)
                     /locus_tag="VFD74_04205"
     CDS             complement(12157..13500)
                     /locus_tag="VFD74_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HZK48784.1"
                     /translation="MDVLESQIAEWRAYVGRSRTIGGPDVDELEAHLRDQMADLGAGG
                     LSADEAFLVAVKRMGDVDGLSREFALEHSERLWKQLVLPARTESHRTSNGLIEALVLA
                     VAAAVAIQIALRGGGWSGEPPPWFLRNVSLFVLPFLAWYFVQRRKLPLLQTLLTAAPF
                     VLGALAVNLYPFRPDSSTEVLVALHLPVALWFAVGYAYVGGEWRSRERRMDYVRFSGE
                     WFIYYVLVALGGGVLMGLTLSVLEPVRAGLGESVIGWVLPSGAAGAVIVAAWLVEAKQ
                     SVVENMAPVLTMVFTPLFALMLTGSALVYAVTGLGGDFDREQLVVFDALLIVVLGLVL
                     YGLSARESTQRAGLMDRIQLVAVAAALVLDVMVLASMLARIGDLGFTPNRVAALGLNL
                     VLFLNLTGAAWLSIRLFSGRATFHRLAHWQTTYLPIFGLWAAIVVVLLPPLFSFA"
     gene            complement(13504..13860)
                     /locus_tag="VFD74_04210"
     CDS             complement(13504..13860)
                     /locus_tag="VFD74_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZK48785.1"
                     /translation="MRIDKDLVAASATPLVLAILSEGESYGYAILKRVRELSGGEFEW
                     TDGMLYPLLHRLHRLGYVTTDWRTPPEGRRRNYYAITDGGRAALAEQQRQWAAVTRAL
                     NDAWTQFNLRPATGGA"
     gene            14124..15281
                     /locus_tag="VFD74_04215"
     CDS             14124..15281
                     /locus_tag="VFD74_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011221458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(5)-(carboxyethyl)ornithine synthase"
                     /protein_id="HZK48786.1"
                     /translation="MNGLTLGIIGTSRKRDEQRVPIHPEHLPRLPERLRRRLIFEEGY
                     GAPFGISDSDIAAQTGGVATRHELLADLGTVVLAKPTLEDIRELREGGILWGWVHCVQ
                     QRALTQAAIDRKLTLIAFEDMYVWGPDGQIGRHTFYKNNELAGYCAVLHALQLKGIDG
                     HYGNQRKTIIFSFGAVSRGAIYALKARGFRDITICIQRPDHEVREEILDCHYVRIRPG
                     DEGQARWLVVEHDGTEFPLTELIGESDIIVNGAFQETDDPILFVTQAEESCLKPGCLI
                     IDVSCDEGMGFFFAKPTTFESPMFKVGKVDYYAVDHTPSYLWESASRSISGALLIHAP
                     AFMEGREAWRRDETIGRAVNIASGVIQKPAILSFQRRRPEYPHEPIDTGTD"
     gene            15618..15872
                     /locus_tag="VFD74_04220"
     CDS             15618..15872
                     /locus_tag="VFD74_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZK48787.1"
                     /translation="MSILAWIVFGGLAGLVAGILTGGGGGIIYDVIVGIVGAILGGFL
                     MSLIGHSGVTGWNWSSFGVAVLGAVVLIVVLRWVRKRGRH"
     gene            complement(15903..17195)
                     /locus_tag="VFD74_04225"
     CDS             complement(15903..17195)
                     /locus_tag="VFD74_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZK48788.1"
                     /translation="MSKPSDGKLGLVSVVAIGVGGMVGGGIFAVLGLAVQLAHGGTDV
                     AFAVAGVVALLTAYSYAKLSVAYPSRGGTVTFLDRAFGAGMFTGSLNILLWLSYVVML
                     SLYAFAFGSYGATFFPEAWQAIGRHALISLIVILITGLNLLSAELIGKAEGWIVGLKV
                     TILLLFVGIGIVHVDASRIAPSSWSSPLQLAAGGMIIFLAYEGFELIANTAHDVRDAA
                     RTLPRAYYIAVGFVIALYVLVALVTVGNLAVDKIVAAQDYALAEAARPFLGQAGFTLI
                     AIAAMLSTASAINATLYGTARVSYSIARDGELPAMLERKVWSQPVAGLFITAGLTLAV
                     ANAFDLTSISTLGSAGFLLIFAAVNAANVRHRRHTNSRGWVSATGAVACLVAFGALVW
                     QTARIGAGRLWVLAAMVALAVLIEGVFRLAKREIRLHE"
     gene            complement(17519..>17800)
                     /locus_tag="VFD74_04230"
     CDS             complement(17519..>17800)
                     /locus_tag="VFD74_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZK48789.1"
                     /translation="ERYDLIFDAVGKRKSASALRRCRQVLAPGGACVSVDDGTPQLRR
                     EDLALLGELATKGEIRPVIDRTYALDDIVDAHKYVDMGHKRGNVIINVA"
BASE COUNT         3408 a         5732 c         5660 g         3000 t
ORIGIN      
        1 tttcatgggg cgccgatgat cgagtaccgt tgacggtcgt cccccgctct cgccgagtcg
       61 ggtgaacgag gaccgtgcga ccggtcccat taccccagac gcggccaacc ggcttccccg
      121 ggaccattgg ccaacccgag tgctacgcgc atcaagaggg ccgcctgcca gggaaacact
      181 gcttggcggg cggctattct caaggagagg attagccatg gcaaagcgtg taagaaagat
      241 atacgaggac actgcggatg tggccaagga gaaggtcgcc gacgccacag gagcggtcaa
      301 agatagggcc atcgaggcgt cggaggtcgc gaagggcaag gcggttgagg ccatagagac
      361 catcaaagac aaggcggtgg atgtgctcga caccgtcgta gaaaagacac gggatctcag
      421 cgaggaagcc cgctccgtgg ccagcgacca actcagtcga ggttcgcgcc aggtcgcctc
      481 ccgggtcaaa gcaacagggg agagcgttca ttccgtcgaa gatgcactgc gacggcacgg
      541 ccaggaacac gccgccgacg tcgcttcgca cgcggcggat cgacttgaga gtgttggctc
      601 acgcctccaa gacgtaggac acagatccag gtggtcgcga ttcagggcct ttcttacgaa
      661 accctggttg ctggtggccg gcgccctgct tgtggccatc gtcgtgtacc gcttcctgaa
      721 gaccccgggc gaacgcgaca cctaaggaga ctgagcgtga ggagatccgg gacgccgggg
      781 gagaacgcgt ggggcctgaa atctggcgaa agagcccatt gcaacaggtg aaacagccca
      841 gaatcctctt gcccaaccga aatgacgtca aaggtatccg caggtgcgtc cccaggcgca
      901 cgagcacttg cagcactgga tcgatcaggc ccgaaagatt cccagcccag agctgcagga
      961 gcaggccctg gcgagttaga gtccacacgt gtggcgcacg aacttctcgg tggtcgcctc
     1021 ttgaaaatga ggtgaccact gctccatcct cgaggggggc ctacttgcca tcggcatgag
     1081 ggcgcagatg gagacgtagg caccgaatga cagacccgct ttcttcaatg ccgggcaact
     1141 caagaagcga aagcgtagaa tgaaggcaga cccaggtgcc cggggcctcc tgcgagcgga
     1201 gttgtgcgtc agttcggagt caagagggag actcaatggt ggttcatagg tcgtcgttgg
     1261 tcattctggt catcgcaatc atcctattgc tgggggcggt cggtctcatc ggctgcgggt
     1321 ctacgcggtc ggcttccacc acgctctccc caggcacagg ggcgacagtc ccactacctt
     1381 ctggcgcgac cacctccctg gagacgacag tttcagtggg cactaccgtc cttggaggcc
     1441 agacccccgc gctcaccttc ctcggaccgg tgaacttgga tacgtggcgt gccctgcgcg
     1501 agattgtcat gaatgctgga gatgacggcc agaaggtgct cgacgaagca gccggactcg
     1561 ctacgggtcc accagtagag gtgggcatcg ccgaacgcga tgcggtgacg ggtatcgggt
     1621 acgcggcggg agccaccacc agtcagcccg ttggcgtcgc catcgatgtt gacgggaagc
     1681 aggttgtagt cttcgctttc agcgtcatag gcgcgccgga cacgacgacc atcgtcggct
     1741 ttgagaggca gacgaacctc gtgggtctgg tcgaggggcc gctaccgatc tccgattcca
     1801 cgggcaacat gaccaccata ccctcaccct gaactggtcg catccccgaa ccgccgcctt
     1861 gcctcgcaag aggtaccggg ggttcgaatc ccccctctcc gccttctagt tcattttcca
     1921 ggtaagcgtg aggaagagtc cggtcgccat ggccgggcgt tcgcattctt gggggcgttg
     1981 ccgagggcct cgccgaagac gagggactca agaggccagg tctccggtga acccgggacg
     2041 ttgtctgatg gtcgttgacc agcaggcggg ctgtgcggta ggcgcttcgg cacccagcat
     2101 tccccccgaa cacgcggcag cgatactttc taagcaagct catgtaggtg gcggcccctt
     2161 cgagccggct cgaaggggcc gccaccacgc gaccggtcgc caaggacatg ctctcaagcg
     2221 tgattctcaa caggaggacg ctggactccg ctcgcggctg acccgcggcc gggagctggt
     2281 ggaaaagggc tatctgcgcc aacggcgcga cgggcgcgct tacttgaccg agccggccag
     2341 aagtccctgt acgaagtagc gttggaaggc gaagaacact atcagcggga tcaccatctg
     2401 aaggaaggcg cccggagcga taacgtcgat gttggagccg aactgacgga gctgatcgcg
     2461 cacgaacacc gtcaggggcg ccgacgatgt gcgggcgaac accaatgcca ccagcaggtc
     2521 gttccacacc cacacgaact gaaagatcag gagcgacgcg atggcgggtg tgcccagggg
     2581 caggaccacg cgggtgaaca caaggaactc cccggctccg tccatgcggg ccgcctccag
     2641 aaggtcacgc ggtatcccga tgaagaagtt gcgcaacagg aagatggcga atggcagacc
     2701 gaaggccgtg tggaacagga ttactcccgg gatggtgccg aacaatccgg tgcgtccata
     2761 caggtcggcc acagggatca atgctacctg caagggaacc accaggagcg caaccaccag
     2821 caggaagagc gcgtcgcgac caggaaaccg catccacgcg aacgcgtagg cggccatggc
     2881 cgcgatgacc accaccaggg tgcacacggg caccgtgatc agcaccgtgt tccatagagc
     2941 atgcagcatg ccggagccct gaaggagcga acggtagttc tcaatcgtga actggctggg
     3001 attggccagg gcggtccacc acccactact cgagttcgcg ggacccgtgc ggaacgaaga
     3061 caccaggagg cccacagcgg gaatcagcca cgccaccccc acgaagagaa gaaagatcgc
     3121 agccgggcct ctagtgacga cccgcaccaa ccggcttgtc cagctccctg ttctcatgat
     3181 cactgctccg cccggaatcg ccgcaggttg aaagccatgg ccggcaggac cagaatgaac
     3241 agcaggatgg cgagagcgct gcccagaccc tgatcctgcc cgccgccaaa ggagacctgc
     3301 cacatctcca aggcgatcac gttcgcgtcg gccaacacgg accccggcgg gatgaccaag
     3361 atcaggtcga agatcttcag cacgttgatg agaagagtga ccaatacgac cagaagcagg
     3421 ggcgcaagca gtggccccgt gatccggcgg aacacctgcc actcccccgc cccgtcgacc
     3481 cgagccgcct ccaatacatc acgagggata gcggccagac cggcgccgat gagcaccatg
     3541 gcgaacccgg cccagatcca gatgtacgag atgatgatgg aaggcgtgat gagtgcgggt
     3601 ccgagccaga ggatgccgcc ccaggcgggc cggaaattgg agtcggcgag ccccaccttg
     3661 taggagcccg cctcgatacc ggtgatcgcg aacgtgccgt caggaccggt cttggtcgag
     3721 ccgatgacat cgccggcatc ggagagcacc tccaccttca ctcccggcag gcccaactcg
     3781 ccggggtcga tcacgcccga tgttcccgta cctccaacgg caaagtccac ccacacggtg
     3841 ccgccgatcg aaccagcctc cgtggacgcc gccaccgcct gcacagcgtc cgaggggatc
     3901 aggcgaggcg gcaccgccac caaaccgagt tgtgcagtcg aaccggctct gtagtccgcc
     3961 gccgtcacat acgcctgagc gtcagacacc agcacgtccg gccgcgacgg cctcgccgtc
     4021 ggatacggtc ccggcggttg aaagaccttc gagaccccga cgagcgccgc gttagccaag
     4081 ccaaggcgcg gatcgtcctg atagacgaga cgccagatta ctcctgacga caggaacgag
     4141 atggccatgg ggacgaagac cgctactttg aacgccgtgg cccaggggac ccgctcggtg
     4201 aggacggcga aaaccagccc cagagcggcc acgatagccg gtgccacaac cacccagatg
     4261 acgttgttct ttatggaggt cagcgtatcc gcggacgtaa acatcgcctg gtagttggag
     4321 atgccgacga actgatcgcc gtgcgcgctg tagaaactgc gcaccagcgt gaagaggatc
     4381 gggtagacga ctaggacacc cagaatgaag atgccgggaa gcaggaagat cgcgaccttc
     4441 cacgacccct gccctccttt gaaccatgaa gcaagacgcc cgcgccccgg cccgcgcgga
     4501 ccacgggtct cggaagtgat gacggctccc gagagatcgg aggcgccgag ggctcccgag
     4561 agatcggacg ggggatcggt ggcgggaacc gccaccgatc ccccgtccgg gtgttgcctg
     4621 tcgctcatac cggttcagtc gtcacggttt gatcggtcgc cactattcgg ctgtcaactg
     4681 cccgaggcgg ccgcggcggc ggcatcctgc agttgcttct gaataccgct gatgtcactc
     4741 gagttcgaga ggaagtcctg cagcagcttc cactcgccct tgcccggggt tccaccgaat
     4801 gccggcggtt ccaggtcgga catgtcgaag cgcaccactt cggcagtggc cagcgcagtc
     4861 gcggccctac gcgtgatgtc gtcgggatat acggacggat cgacgttctt gttcggagag
     4921 ctgaagcccc cgagagccgc ccagatggtg gctgcttccg gagtcgcaag gaacttcatg
     4981 aaggccttgc cggcatcaga gtccttcatc agcaccgcca tgtcaccgcc gccaaccact
     5041 gaggcgggcg agccgttgat agacgggaag tcgaagaagt cggcaccggt cccaagagtc
     5101 gcctgcgtct cggagccgat gactccggcg acgaagtcac cttcatacac catcgcggct
     5161 ttaggcggcg tggtaaacgc gttcaccacg gaagtcggga agtcggtctg cagggcaccg
     5221 gtagctccac cgctcagcat ggcgtcgctg ccccagatct tggccagctg ttcgagcgcg
     5281 gtggtcacac tcggatccgt ccacgggatc tcaagagtgg tcagctgatc atacttttcg
     5341 ccgcccgcgg tccgcaggta gacgttctcg aaccagtcgg tcagcgtcca accgtcggct
     5401 cccgccacag agaacggagg tacaccgaag cgctgtatgg tgtcggccgc cgcaagcaac
     5461 tcgtcccatg tagtgggcgg agtcacgccg gcgtcgctga acacacccat gttgtaccag
     5521 aaggtggatt tgttggccgc cttgaagacg aggccgtact gggtgccctc atgagtgccg
     5581 agatccttcc agaccggcgc gaagttggcg tccatctcca taccgatcac atccgtcaga
     5641 ggctggagcg caccttgatc cgcgagggag tacatgaggc ccggctgtgg caagacggcc
     5701 acgtcgggcg ggctgccacc ctgcaggcgc gtgccaagga tcgtggcgat ctggtcgcct
     5761 gtggacgtgt atgtcacctt caccccggtc tgctcctgga acgcgtcgat gaccttctgg
     5821 aagttgtcct gctccgcgcc gctccacacg gcggcgacct ccatggtctg cccgctgagg
     5881 tcgggggccg aggtcgtgct ggtctcggga gccgcgctcg cggtcgtttc tccgccggct
     5941 gctgtagtcg tggtagttgt gccgccggag cacccgacga cgaccaggag tagagctgcc
     6001 agcagtagca cgatggttgt tcgtccgatc cgcatgatga tgaatcgcct ccttctgcga
     6061 gcgtccgtcc tgctacgacc cgtagacgcc tagacccgtt tccggatcga aggcgtgcac
     6121 tctggccgtg tcgacggcaa gctccatcgg ttgaccgacc gccgcccccg tttggggact
     6181 gacggcggcc acgaacttgg acttcccctg ttccctttcc cgctccagtc tctcgagctt
     6241 ctcggctccc acgtccaccg ccagttcacg cacgtcctcg gtaaggacga tgggcgcgtc
     6301 gatggtgaag tgaatgagcg cttccgaacc gagcgactcg atgaggtcgg ggatcaccct
     6361 catccgcctc tcggccgggg cctcttggag tgctgcgtcg tgcatgtcct ctggtctgat
     6421 gccaaggacg atggtgcgac cctcgaactt cttcagatcc ggccgggtgc tttcgatctt
     6481 ctcgtccaaa cgcagacgca tgccgccgaa acggacgaag agcccaccat cttcgcggat
     6541 gaccgtcgct tccaccatgt tcatggcggg ggagccgatg aatccggcca cgaagagatt
     6601 gacgggatga tcgtacagag tctgtgagga atcgacctgc tgcagccgac ccttcttcat
     6661 gacggccacc ctgtcgccca gggtcatggc ctcgacctgg tcgtgcgtca cgtagatcgt
     6721 agtgacgttc agatcatcct ggatacgggc tatctgcgct ctcgtctcga cccgcagttt
     6781 cgcgtccagg ttcgatagcg gctcgtccat gaggaaggcc tggggttccc gcacgatggc
     6841 ccgtcccatg gccaccctct gccgctggcc accggacagg tttctcggct tgcgacccag
     6901 cagttggtca aggcccagga tgcgcgcggc ttcgtgcacc ctcttgtcga tctccgcctt
     6961 gtcgaccttg cgcatcttca gtgagaaggc catgttgtcg tagacggaga ggtgggggta
     7021 gagagcgtag ttctggaaca ccatggccac gtctcgatcc ttgggggcca tgtcgttgac
     7081 gacgcgacct ccgatgctga cggtgccccg agtgatgtct tccaggccgg ccaccatgcg
     7141 aagggcggtc gtcttgccgc atccggacgg gcctacgaac accatgaact cgccgtcttg
     7201 gacctcgagg tccatatcct ccacggccag ggttccgtcc gcgtacacct tggtgagctt
     7261 gtctaagtag atctctgaca tccgctcccc gcctagtctc gccggtgcat ctcccaacga
     7321 ctctcagcga ctttcagcga cgcccgggtt gcgattcttt tcccaagcag catcgcctgc
     7381 aaacccgtgt ccccctgccg acgtccaagc aaaccattcg ttcccgagag tcagcccagc
     7441 tcgaggatgc cggaacgaag ctcgactgag tggacgagca catcgatcgg ctcaccgatg
     7501 ccatggagga gcaactcggc cctttcgtcc aggccgtcgg gctcgcgggc acgatccccg
     7561 gcggcgatac gagcgtggtc cgccggcacg gtcggcggcc gcccagggtt taggcgactc
     7621 ccgcgagggt gagtatcgtt gtcatggtcc ttttgtcatg gtcctttgtc atggtcgtac
     7681 ttctcaggtt gtcgtgatcc tgcatctgag acccggtggg gggatatcat gaacgtgttg
     7741 caggccgtgg ggcatgccct gtcgatcgcg ggactcatga cgtggcagat cctgtgggcg
     7801 ctgatactgg ggttcgcgct ttcggcgatc attcaggcgc tggtgcgccg cgagaccgtc
     7861 gtcgggctgc tgggtgacga tcggccccgc acgctcgtcg tcgccgccgg ggtcggcgcc
     7921 gcctcgtcgt cgtgctcgta tgcgtcggtg gcgctcgcgc gcacgctctt catgaagggt
     7981 gcgaacttca ccgctgcgat gacattcgag atcgcttcca ccaacctagt ggtcgagctc
     8041 ggcatcatct tggcgctctt gctctcctgg cagttcaccc tggccgagtt cgtcgggggg
     8101 ccgctgatga tcgtcatgtt ggcggtactc ttccgactcc tattgcgcaa gcgactgctc
     8161 gacggcgccc tcgcccaggc caagaaggga ctcgccgggt ccatggaggg tcacgccgcc
     8221 atggacatga gcgtgcgcga gactggaagc atctggaaga ggctgcggtc gccgagtggg
     8281 ttcacatcgg tctcgcacgt gttcgtcatg gagtgggcgg cggtgctgcg cgacatcgtg
     8341 ggggggctgc tcatcgcggg agcgatcgcc gcctgggtac ccgcgtcctt ctggcgaatc
     8401 ttcttcctga cggaggaccc tttgctcagc aagatctggg gtccgatcgt cggccccttg
     8461 gtctcggttc tgagcttcgt ctgctccatc gggaacgtgc cgctcgcggc agtgctctgg
     8521 accggtggca tcagcttcgg aggggtgatc agcttcatct tcgccgacct catcatcctg
     8581 cccatcctcc tcatctatcg gaagtactac ggcacgaaga tgatgctgtt cctggcgggc
     8641 accttctacg tcaccatggt ggcggccggt ttcctggtag aggtgatctt cggcgtcctg
     8701 ggcctcgtcc ccacggagcg cagttcgcag gtcgtggagt cccacatcag cctcaactac
     8761 acgagcgtgc tcaacgtcgt cttcctggcg ctcgccggtg tgctcctgtg gcggttcttc
     8821 cgcaccggcg ggcgcgagat gctcgccatg atgagcggcg cacccggggg aatggcgggc
     8881 cacgaggagt cgactcatga gtgacggccc cgcgggcgga gcggatgccc ccgaagcgaa
     8941 agaagcggcg tgtctggtcg tggactccga ccgcttcgac gccgtgctgt tcgacatgga
     9001 cggcgtgctc accacgaccg cgcgcatgca cgccgccgcc tggaagcgta ccttcgacgg
     9061 gttcctcgac gactgggacg cggaacacga ttctcacact ccccggttct ccgtagccga
     9121 agactacccg acctccgtcg acgggaagcc gcgtcaggag ggtgtgcgcg acttcctcgc
     9181 ctcgcgggac atctctctgc ccgcgggcac gccggactct cctccgaccg agtggtcggt
     9241 ctacggtgtg gggagccgca agcaggagtt cgtcgaggag gccctgcggg cggaaggagt
     9301 cgaggtcttc ccggggtccg tcgcgtggct gagggagctg agggagatgg gcatcggaac
     9361 ggccgtggtc acctccagca ggaactgcga cgtcatactg agcgcagccg gcatcgccga
     9421 cctgttcgac gcccaagtgg acggcaacac ggcgatcgag ctcgggctga cgggcaagcc
     9481 cgccccggac atcttcctgg aagccgcgcg ccgcctcggg gccgcgccgg cgcgggcggt
     9541 ggtggtcgaa gactcccaag ccggcgtcga agcaggccgc gcggggggct tcggcttggt
     9601 gatcggggtc gatcgcgccg atcaggccga cgagttgcgg caggcggggg cggatatcgt
     9661 cgtcgcggac ctggccgagc tcgtcccctg cgagcccgac gtgcgccatc cggccgggcc
     9721 gaggcggcac cgtctgcgcg cgagcgccat ccgcctggtc gccgcggagg gtgactttcc
     9781 cgtcgacgaa caccgcttgg tagagcgtcg cttcaacccc cagtacgttc cccagctgga
     9841 gagcatcttc gccctcgcca acggctacct gggcctgcgg ggcacccaag acgaaggtcg
     9901 tccggtctac gatcccgctc ccatgctgaa cggcttctac gagacctggc ccctggtgta
     9961 cccggagcgc gcccacggat tcccggacac cggacagacc atattgggag tgccagacgg
    10021 cgcaatactg cgcctgtacg tcgacgacga ggccttctcc ctggaacacg cagacgtcct
    10081 cgagtacgag cgggtcctcg acatgggcgc aggcaccctc gagcgacggg tggtgtggcg
    10141 cgcgaccgac gggagtcggt tctccgtcag gtcccggcgc ctcgtctcgt tcgagaaccg
    10201 gcacttggcc tgcatacagt acgaggtgat atctctcgac cgagccgccg acctcaccat
    10261 ctcttccgac ctcatgatcc accctgagaa cggttctctc gactccaagg atcctcgtcg
    10321 caaccaagcc ctgggaccag gagtgctcca aaagaccggg gagcacgtca acgatcagcg
    10381 ggtggtcctg gcctaccagg ctcgggcgag cggattgcag atggcctgcg gcatggacca
    10441 ccggctgacg agcggcacgg cggcggcacc accccaggca gtcgtcgaag gagattgggc
    10501 gcgggtcgtc taccggatcg cagccgggca gggcgagccc gtgcgcctcg tcaagtacct
    10561 ggcctaccac tacgacggcc aggagccggc cgcggagttg tctttccgcg tgggccagac
    10621 cctcgaacgc gccgcggccg agggcatcga cagcgtcttc gccgcccagc acgcctatgt
    10681 gcaggacttc tggacccgca gcgacgtcat aaccgagggt gcaccgctgt tgcagcaggc
    10741 ggtccgcttc aatctattcc agctgctcca ggcttccgcc cgcctagagg gacacggaat
    10801 gccggccaag ggcctgaccg gtcgaggcta tgagggccac tacttctggg atgccgagat
    10861 ctacgtcatg ccattcctca cctacacggc tcctcatatc gccaggaacc tgttgctcaa
    10921 ccggtacgcg atgctcgata aagcccggcg aagagccctc gaagtgagtc agcagggagc
    10981 gctgtttccc tggcggacga tcaacggcga ggaagcctcc gcctactatg cggccgggac
    11041 cgcccagtac cacatcgatg cggacatagc ctacgctctc acgcagtacg tccgcaccac
    11101 ggaagacacc gagttcctcg cccgccaggg cgccgagatg ctggtggaga cggcccgtat
    11161 gtgggtcagc ctgggcttct tctccgaccg caaggacggc cgcttcgtca tcaacggggt
    11221 caccggaccg gacgagtaca gcgctctcgt cgacaacaac accttcacca atctgatggc
    11281 gcaagagaac ctgcgccagg cggtgcgcgt cgtggagtgg ttgggcgttt cgaacccgga
    11341 agcccacgca ggcctggtcg agcgcaccca actcgacgac gcggagctcg agctctggca
    11401 acgggcggcc gacctcatgt acgtccccta cgatcaagaa ctgggtctgc acctgcagga
    11461 cgacggcttc ctcgatctcg aaccctggga cttcgagaac acccctctgg aggactatcc
    11521 gctccttctc cattaccatc ccctggtgct ctaccgccac caggtgatca agcaggcgga
    11581 tgtggtcctc gccaccttcc tcctcggcga cctcttcacc gccgaagaca agcggaagat
    11641 cctcgactac tacgacccgc tcaccaccag tgactcctcc ctgtcggagt gtatccaatg
    11701 catcatggcc gccgaggtcg gtgacgatct gcgcgccgcg gaggagtact tcatcgacgc
    11761 agtggggatc gacctaggcg atgtggcggg caacgtgcgt gacggcgtgc acgtcgcctc
    11821 ggccggaggc acgtggatgg ccttggtcta cggcttcggc gggatgcgag accggaatgg
    11881 agaactctcc ttccggcccc gcctgcccgg gcgcatgact cgcctgtcgt tcaaagtgca
    11941 cccgaggggc gcgactctgg aagtcgacat caccgcagcg ggagcgacgt atcgcctgga
    12001 atccgaggac gaactgacca tcgtccactg cggggagagt ctgcgtcttc gatccgacat
    12061 ccccgtcacc cgacccctgg tcacgcgcgt ccaagaggac gacccaacga gcgtgagccg
    12121 gccgtgagac gtccactgtg cccggacagg ggccgactat gcgaaggaga agaggggcgg
    12181 aaggaggacg acgacgatgg ccgcccacag gccgaagatc ggaaggtacg tcgtctgcca
    12241 gtgagcgagc cggtggaagg tggcccggcc ggagaacaag cggatcgaga gccaggccgc
    12301 cccggtcagg ttcaggaaca gcacgaggtt gagaccgagc gcggcaacgc ggttaggggt
    12361 gaacccgagg tcgccgatcc gggcaagcat cgaggcgagc accatcacgt cgagaaccag
    12421 cgcggcggcg accgcaacaa gttgaatgcg gtccataagg ccggctctct gcgtcgattc
    12481 tcgtgccgaa agcccgtaaa gcacgagtcc gagcaccacg atcagcaagg cgtcgaacac
    12541 gacgagttgt tcccggtcga agtcaccccc cagcccggtc accgcgtaga cgagggcgga
    12601 gccggtcagc atcagcgcga acagcggggt gaagaccatg gtgagcaccg gcgccatgtt
    12661 ctcgacgacg ctctgcttcg cttcgaccag ccacgccgcc acgatcacag ccccagcggc
    12721 tcccgatggg agcacccacc cgatcacact ctcgcccaga ccggcccgca ccggttcgag
    12781 gacggacagg gtgaggccca tcaacacccc tccaccgagg gcgacgagga cgtagtagat
    12841 gaaccactcg ccgctgaacc gcacgtagtc catgcgccgc tcgcgagatc gccactcgcc
    12901 gcccacatac gcgtagccga cggcgaacca cagcgcgacg ggcagatgaa gcgccacgag
    12961 cacttcggtg gaggagtccg ggcggaaggg atagaggttc acggcgaggg cgcccaacac
    13021 gaacggcgcc gccgtcagca gggtctgcag aaggggcagc tttcgccgtt ggacgaagta
    13081 ccaggcgagg aacggcagca cgaacaggct gacgttacgg aggaaccacg gcggcggctc
    13141 accggaccat ccacctcccc tgagtgcgat ctgaatcgcg acggcggcgg cgaccgcaag
    13201 gacgagtgct tcgatcagac cgttcgaagt gcggtgcgac tcggttctgg ccggcaggac
    13261 gagctgcttc cagagccgtt cgctgtgctc cagcgcgaac tcccgggaca gcccgtcgac
    13321 gtcgcccatc cgtttgacgg cgaccaggaa ggcctcgtcg gcgctgagac cacccgctcc
    13381 cagatcggcc atctggtcgc gcaggtgggc ctccagctcg tcgacgtcgg ggccaccgat
    13441 ggtccgggag cgcccgacgt aggcccgcca ctcggcgatc tgcgactcga gaacgtccat
    13501 gtctcaggct cccccggtcg cgggcctgag gttgaactga gtccaagcgt cgttcagtgc
    13561 ccgcgtgacc gccgcccact gacgttgctg ctcggcgagg gccgcccggc ccccgtcggt
    13621 gatcgcgtag tagttgcgcc gacggccttc gggcggggtg cgccaatcag tggtcacgta
    13681 gccgaggcga tggagacggt ggaggagcgg gtagagcatc ccatccgtcc actcgaactc
    13741 cccccccgac agctcccgca cgcgcttcag gatggcatat ccatagctct caccctcact
    13801 gaggatggcg agcacgagtg gcgtcgccga ggcagccacc agatctttgt cgatacgaat
    13861 cggatcaccc atgtcacccg gtactctagc actactatac atagacgtgc aaggcgtaag
    13921 acgactggtc ggaggccctc tattcctgcc cggtgcgatt cgttccatgg aagcccttga
    13981 accggctgcg ccgcacacaa ccccgcgctc cggtgaaccc ggcgtgattc gatgcggtag
    14041 agtggggtga gctttacata cccgttgacg caccaagcgt agaggccact ctccgatcgg
    14101 agagtgacac ataaggaagc aagatgaacg gactgacact ggggatcatc ggcacttcaa
    14161 ggaaacgcga tgagcagcgc gttccgatcc atccggagca tctgccgcgt cttcccgagc
    14221 gcctccgccg ccggctgata ttcgaggagg gctacggagc gccgtttgga atctccgact
    14281 ccgacatcgc cgctcagacc ggcggcgtcg ccacgcgcca cgaactgctg gccgatctgg
    14341 gcaccgtggt gctggcgaaa cccaccttag aagatatacg ggagcttcgc gagggtggga
    14401 tcctgtgggg gtgggtgcat tgcgtgcagc agcgcgccct gacacaggct gcgatcgatc
    14461 ggaagctgac gctcatcgcc ttcgaggata tgtatgtttg gggacccgat gggcagatcg
    14521 gccgccacac cttctacaag aacaacgagc tggccgggta ctgcgcggtc ctgcacgcgc
    14581 tgcagctgaa gggcatcgac ggccactacg gaaaccaacg caagacgatc atcttcagtt
    14641 tcggtgcggt cagccgaggg gccatatacg ccctcaaggc gcgtggattt cgagatatca
    14701 cgatatgcat ccagcgcccc gaccatgagg tgcgcgagga gatactcgat tgtcactacg
    14761 ttcgcatccg gccgggcgac gagggccagg cgcgctggct ggtcgtggaa cacgacggca
    14821 ccgagttccc gcttacggag ttgatcggcg aatcggacat catcgtgaac ggagcctttc
    14881 aagagaccga tgatcccatc ctcttcgtga cccaggcgga ggagtcatgt ttgaagccag
    14941 ggtgcctgat catcgatgtg agttgcgacg agggtatggg gttcttcttt gccaagccaa
    15001 ccacgttcga gagcccgatg ttcaaggttg gaaaggtcga ctactacgcc gtggaccaca
    15061 caccgagcta cctctgggag agtgcctcga gatcgatctc cggcgccctg ctgattcatg
    15121 cgccggcttt catggagggc cgtgaagcct ggcggcgcga cgaaaccatt ggacgggcag
    15181 tgaatatcgc ttccggggtg atccagaaac ccgcgatcct ctcgtttcag aggcgccggc
    15241 cggagtatcc tcacgaacct atcgataccg ggacggactg acggatcgca aacgcgtatg
    15301 gtgcccaacg cattgacctt ccctgggcca agacatgagt cccggttcgc ccgaaggcgt
    15361 acgaccgcgc gcgcaacagc ccggcaccgt ctgagtgctt tgtcaatacc cgggcgcctg
    15421 cctcgccgag gactccgcaa cctcaccgat gccacaaccg ccgacgcggc cgcggcacac
    15481 cgactcggcg ccctcgcgat tcgcgggccg gtttcggcca ttaaggtgcg ggatactccg
    15541 tggggcaagc cgcgtgagta cgcggcgacg ggggcaggtc gccccggcaa ctcaactcca
    15601 taccgagagg ttacgtcgtg agcattctgg cgtggatcgt ctttggtggc ctcgccggct
    15661 tggtggccgg gatcctcaca ggcggaggcg gaggcatcat ctacgacgtc atcgtcggca
    15721 tcgtgggggc gatactgggt gggttcctca tgagcctgat agggcactca ggcgtgaccg
    15781 gttggaactg gtcgagtttc ggggtcgccg tgttgggagc ggtggtgctg atagtcgtcc
    15841 tgcgctgggt ccgaaagcga ggaagacact agcacccgag gagatgccgg cgccggaagc
    15901 agtcattcgt gcaatcgaat ctcccgtttc gccagccgga agacgccctc gatcaacacc
    15961 gccaaagcaa ccatggccgc gaggacccac agccggccgg ccccgatccg agccgtctgc
    16021 cagaccaatg cgccaaacgc aaccagacac gccacagctc ccgtcgcaga aacccagcct
    16081 cgactgttcg tgtgtcgccg atgtcgcaca ttggcggcgt tcacggcggc gaagatcagc
    16141 agaaacccgg cgctgcccag cgtcgagatg ctggtgaggt cgaaggcgtt ggccacagcg
    16201 agcgtcagcc cggcggtgat gaatagtcct gccacaggct gactccacac tttccgctcc
    16261 agcatcgccg gcaattcgcc gtcgcgcgcg atgctataac tcacccgagc cgtcccgtag
    16321 agagtggcgt tgatcgccga agccgtagat agcatggcag cgatcgcaat cagcgtgaag
    16381 ccggcctgac cgagaaaggg tcgagccgcc tctgccaacg cgtagtcctg agccgccacg
    16441 atcttgtcca cggcgagatt gcccacggtc acgagcgcga ccagcacgta caacgcgatc
    16501 acgaagccca cagctatgta ataggcacgt ggaagcgtcc gggccgcatc ccgcacatca
    16561 tgtgcagtgt tggcgatcaa ctcaaagcct tcgtaagcca ggaagatgat cattccgccc
    16621 gccgccaact ggagaggcga ggaccacgaa ctaggggcga ttcgggaggc gtcgacgtgg
    16681 acgatgccga taccgacgaa caacaagagg atcgtcactt tcaatcccac gatccaaccc
    16741 tccgccttgc cgatcaactc agcgctcagc aggttcaggc cagtgatcag gatcacgatg
    16801 agactaatca gtgcatgcct gccgatcgcc tgccacgcct cggggaagaa cgttgcgcca
    16861 tagctaccaa aggcgaacgc gtacagcgac agcatcacga cgtagctcag ccacaacaga
    16921 atgttgaggc tgccggtgaa catgccggcg ccgaacgcgc ggtcgaggaa agtgaccgtt
    16981 ccaccacgac tcggatacgc gacggaaagc ttcgcatacg aatacgcggt cagcagtgcc
    17041 accacgcccg ccacagcgaa ggccacatcc gtgcccccgt gcgccaactg caccgccaac
    17101 cccagcaccg caaagatccc gccgcctacc attccaccga cgccgatggc aactacgctc
    17161 accaacccaa gcttgccgtc acttggtttg ctcatgtggt ggatatctcc actttgcctg
    17221 aagggcagct gcaggtgatg ggtctcgccc tgggtggctt tctctttgta tgagtataca
    17281 aggtgggcca catcggccaa ccttgcatcg gacggaagct ttgcaggttg cccgccgacg
    17341 aaaagccgga tggtacggct ggccgcgcaa caggccggcc ccgtgtcaaa cctgcgcctc
    17401 agacagatgg catacgcttt gagggacgag gaggtggccg ggttcgccgc cgaagcgcca
    17461 gacgctacgc gacaaggagc ggaggtgggt gaacggcaat agggagaaag tgagcggact
    17521 aggccacgtt gatgatcacg ttgccgcgct tgtgacccat gtccacgtac ttgtgcgcgt
    17581 cgacgatgtc gtcgagggca taggtgcgat cgatcaccgg cctgatctca ccctttgtgg
    17641 ccagctcgcc gagcagcgca agatcctcgc gccggagttg cggtgtgccg tcgtctaccg
    17701 agacgcaggc tccgccgggg gcaagcactt gccgacaccg ccgaagagcg gaagcgctct
    17761 tcctcttgcc cacggcgtcg aagatcaggt cgtagcgctc
//
LOCUS       DASRMJ010000151         7611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_128560, whole genome shotgun sequence.
ACCESSION   DASRMJ010000151 DASRMJ010000000
VERSION     DASRMJ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128560"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFD74_04235"
     CDS             complement(<1..355)
                     /locus_tag="VFD74_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZK48790.1"
                     /translation="MIDATVPTISVEEAVGGDYVIVDVRSPSEFAHSHALGAVNLPLL
                     GDRERAQVGTVYAEGGATAARMTALDLISADLPSYMRSLKAVSARGRRPAVMCWRGGE
                     RSRNVVMLLSLVGVHA"
     gene            complement(358..1332)
                     /locus_tag="VFD74_04240"
     CDS             complement(358..1332)
                     /locus_tag="VFD74_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK48791.1"
                     /translation="MDIGHALGVVLDILSAPSTYQAMLRISTPLALAAIGGTFCERTG
                     VVNIALEGIMLVGAFAGAVFSYYAQASWGFSEAMAPWVGLIGAVTTGMLFSLLHGLGS
                     IHLRADQVVVGAALNIFALGATGFLTQAIFGVAGASPRVASFDPIAIPGLARIPFLGP
                     VLFNHTPIVYMAAVIAVAGQFILFRTPLGLRLRAAGEHPKAADTVGVSVYRMRYIGVL
                     ISGALGGLAGANLSLESVSQFSENMTRGRGFIALAANIFGKWTPLGSWLSSLLFGFAE
                     SLKLRTQVIDLPPEFILMMPYVLTLVVLAGAMGRAVPPDAIGKPYSKQ"
     gene            complement(1332..2507)
                     /locus_tag="VFD74_04245"
     CDS             complement(1332..2507)
                     /locus_tag="VFD74_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK48792.1"
                     /translation="MSDRSGVVPGAAGGARRRRIFVAAKRVGLSLMQPVASVIIATVI
                     GGVVMAAFGFDPFSGYAGLFRYAFLSEFNFTVTVLNSIPLIFTGLAVAFAFRCGLFNI
                     GAEGQLMVGSLFAGWLGTLISLPMVITVPLLLIVGAVTGALWALLPGILKATTGAHEV
                     ITTMMMSWIGLYLTSYLVKNVLMDPRTTLPASNLISEQARLLTLNRVLPFLFRSPTRL
                     HLGVVLAVLAAVFIWYLLTRTSLGFEVRAVGSNPFAAEAGGVSVRRNVIVALLISGAL
                     AGLGGTVEVMGLHYRVYDHFSAGYGFTGITVALLAKNHPLGVIPAALLFGALTNGGAG
                     MQLKAGIPIDIVDVLSGVIIFLVAAEELFNALKRRQEKRSLLSRSMGGAEIDGRSEA"
     gene            complement(2500..4056)
                     /locus_tag="VFD74_04250"
     CDS             complement(2500..4056)
                     /locus_tag="VFD74_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48793.1"
                     /translation="MEEHIQTSRGPESADTVVRLTGISKFFPGVVANDRIDLDLRRGE
                     IHALLGENGAGKTTVMNVLYGLYEPDAGAIEIEGRQVRIPSPRTALGLGIGMVHQHFM
                     LIQKFTIAENVVLGHEPHTRARTLDLREARRRLRELSDTFGLEVDPDRKVMEASVGMQ
                     QRAEILKALYAGAKILILDEPTAVLAPQEVDDLFGTVRSLTKQGLSIFFISHKLEEVT
                     EISDRVTVIRRGRVVGTLDTPSTTKRELAQLMVGRDVVLQVEKEEHAAGKEVLRVEDL
                     VVRDQRGLTALKGVSFAVREGEILGVAGVDGNGQIELEEGIIGLRKSASGRILFGEED
                     ITSHGVRGRIDRGLGLVPQDRQKRGLVLDFDLAENILLGNQWKRPYARLLGLDYDYIR
                     AQARSAVEEFDVRTPGIEVSARTLSGGNQQKLILAREIRRGPRVLVAAQPTRGLDVGS
                     IEFVHSRLLELRAKGVGIILISYELEEILSLADRIIVLYEGEVVYQVAGAGAARETIG
                     LAMTGGLAGE"
     gene            complement(4212..5279)
                     /locus_tag="VFD74_04255"
     CDS             complement(4212..5279)
                     /locus_tag="VFD74_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HZK48794.1"
                     /translation="MSRRKLVVFLVVVLALGTLTVVGCGGDSGSKDGAYKAAMVTDVG
                     KLGDKSFNDGVWAGMEQAKADLGAETSYLVSEQQTDYVPNLTRLTEDGNPLVVAVGVL
                     MTDALREVATAHPDTLYAGIDIDLVDKDYNPIDLPNVREVLYAEQQVGYLAGLVAGAM
                     TLEAGASDRLNADKVVGLVAGMKVPSVDRYIAGYIDAVKSIDPDIKILLTYTDTFIDP
                     TAGKEAALPMYENGADIVFQVAGKTGLGVFEAAKDTGHFVIGVDVDQSPELPEVILVS
                     AMKNLQMGAFVAVKDASEGNFDTGNFVYDLKNDGVGLSSFHDFDSIIPQTLKDLIEKS
                     KADMVSGALAPATIVDESLVN"
     gene            complement(5474..>7611)
                     /locus_tag="VFD74_04260"
     CDS             complement(5474..>7611)
                     /locus_tag="VFD74_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZK48795.1"
                     /translation="AIVGWLLMIGGLSMVTGVTIRRFVLGTGRAAKAAQVGAQERTRR
                     LRERAVTTDDRGPLDDPYLAGFGSARPARQGGAPGTAATEPGVVSGGGEWPSHPSDPD
                     TTVTGRSWDTPLERVADTRSGPDLIDGAQAFPDIYPLGSSSSSFGGSSGLPESSLLTA
                     GTGDAPGRGRLQAVDDDGGEGEIVATTAERAQATGVPVQGILPGLGQPEARRASVAAA
                     PYYALPDSEILKKSAAPAERSDRADIEIADQLVLTLSHFGVEARVIGTVIGPRVTRFE
                     LQLAPGTKVSKVSSLKDDLAYALASTEIRILAPIPGKQAVGVEVPNQRPTFVTLGDIY
                     KDFPSTAGPLMVWLGKDISGKSVHADLARLPHLLIAGTTGSGKSGCVNCIISSILLRA
                     SPDKVKLILIDPKKVELSHFEYVPHLLVPVVTNMKNAAGVLHNIVKEMENRYELMELE
                     KARNLEEANKARARRGERPLPFVVVVIDELADLMMVSPGEVEEYIIRLAQKARAVGIH
                     LVVATQRPSVDVITGMIKANIPSRIAFAVSSQTDSRVILDSNGAEALLGMGDMLFKPL
                     GSNRLQRVQGAFITEEEIHRLVTHCRRQAEPVYQEELLLPPQPTSKRDDDDFDPDSDD
                     LLLDAAEMVLETGSASVSMLQRRLRVGYTRAGRLIDMLERRGIVGPYEGSKARQILVG
                     PGESERVLETLKAVGGATEAPDEAEDFDG"
BASE COUNT         1403 a         2583 c         2312 g         1313 t
ORIGIN      
        1 ccgcgtgcac ccccaccagc gagagcaaca tgaccacgtt gcggctccgc tctcctcccc
       61 gccaacacat cacggccggg cggcgccccc gggcggatac tgccttcagg ctcctcatgt
      121 aggaggggag atccgcgctg atgagatcga gggcagtcat cctggcggcg gtggcaccac
      181 cctcggcgta gacggtgccc acctgcgcgc gctcgcggtc gcccaggagc gggaggttca
      241 cggcacccag cgcatggctg tgcgcgaact ctgagggaga gcggacgtcg actatcacgt
      301 agtctccgcc tacggcctcc tccacggaga tcgtgggcac ggtggcgtcg atcacggtca
      361 ctgcttcgag tagggcttgc cgatggcgtc cggcggcaca gcccgaccca tggcgcccgc
      421 caagacgacg agggtgagca cgtagggcat catgaggatg aactctggag gaagatcgat
      481 cacctgcgtg cgcaacttca gagactcggc gaacccgaac aggagtgagg agagccacga
      541 ccccaacggg gtccacttgc cgaaaatgtt ggcggcgagg gcgatgaaac cgcggcccct
      601 ggtcatgttc tccgagaatt gagacaccga ctccagcgag aggttcgcgc cggcgagtcc
      661 accgagtgcg ccggagatca gaacgccgat gtagcgcatc cggtagaccg agactcccac
      721 cgtgtcggcc gccttgggat gctcaccggc ggcgcgcagc cgaaggccca aaggtgtgcg
      781 gaacaggatg aactgcccgg cgaccgcgat caccgcggcc atgtagacga tcggggtgtg
      841 attgaacagc acaggcccca ggaaggggat gcgcgccaat ccggggatag cgatgggatc
      901 gaaggaggcc accctcggag acgccccggc gacgccgaag atcgcctggg tgagaaaccc
      961 ggtggctccg agggcgaaga tgttgagagc cgcccccacc accacctgat cggctcgcag
     1021 atggatgctg cccaggccgt ggagcaggga gaagagcatg ccggtggtca ccgctccgat
     1081 cagccccacc cacggcgcca tggcttcaga gaacccccac gatgcctgtg cgtagtaaga
     1141 gaagaccgcc cccgcgaagg cccccaccag catgatgccc tcgagcgcga tattgacgac
     1201 tcccgtgcgc tcgcagaacg tccccccgat cgccgccagc gcgagcggcg tcgagatgcg
     1261 gagcatcgcc tgataagtgg aaggtgcgct caggatatcc agcaccactc cgagggcgtg
     1321 gccgatgtcc atcaggcttc gctcctgccg tcgatctcgg cgccgcccat cgaacgcgag
     1381 agcagactgc gcttctcttg cctccgtttc agcgcgttga acaactcctc agccgcgacc
     1441 aggaagatga tgaccccaga caggacgtcc acgatatcga tggggatgcc ggccttcagc
     1501 tgcatccctg ccccgccgtt tgtcagggcc ccgaagagca aggccgccgg aatgacccca
     1561 aggggatggt tcttcgccag gagggctacc gtgatgccgg tgaagccgta gcctgcggag
     1621 aagtggtcgt acacgcgata gtgcaggccc atgacctcca ctgttccgcc caaccccgcc
     1681 aacgcgccgg agatgagaag cgccacgatc acattgcgcc gcaccgagac gccgcctgcc
     1741 tccgcggcga acgggttgga ccccacagct cgcacctcga acccaaggct ggttcgcgtg
     1801 agcagatacc agatgaagac agcggcgaga accgccagaa ccacgccaag atggaggcgc
     1861 gtaggacttc ggaacagaaa cggcagcacc ctgttcaatg tgagtagtcg cgcttgctcg
     1921 ctgatcaggt tcgacgccgg aagtgtcgtt cgcgggtcca tcagcacgtt cttcaccaag
     1981 tatgacgtga ggtacagacc gatccacgac atcatcatgg tcgtgatgac ttcgtgcgcg
     2041 cccgtcgtcg ccttcaggat gccggggagc aatgcccaca gggcgcctgt caccgcacca
     2101 acgatcagca ggagggggac cgtgatcacc atcggcagcg agatcagagt gcccagccag
     2161 ccggcgaaga gactcccgac catcagttgg ccctctgcgc cgatgttaaa aaggccgcaa
     2221 cggaacgcga aggccaccgc aaggcccgtg aagatgagcg gtatggaatt caacaccgtg
     2281 acggtgaagt tgaactctga gagaaaggcg tagcggaaca gcccagcgta gccggagaac
     2341 ggatcgaagc cgaacgcggc catgaccaca ccgccgatga cggtggcgat gatgacggac
     2401 gccaccggct gcatcaggga gaggccgaca cgcttcgcgg ccacgaagat ccgtcgcctt
     2461 ctcgccccac cggccgcccc aggcacgact ccgctcctgt cactcaccgg cgagcccccc
     2521 cgtcatcgcc aagcctatcg tctcgcgcgc ggccccagcg ccggcgacct gatagacgac
     2581 ttcgccttcg tacaggacga ttatccgatc ggccagcgac aggatctcct ccagttcgta
     2641 ggagatgagg atgatgccca cccccttggc gcgcaactcg agaagtcgcg agtgaacgaa
     2701 ctcgatcgac ccgacatcga ggccccgcgt gggttgcgcg gcgaccagca cgcgcgggcc
     2761 ccttctgatt tcgcgggcca ggatcaactt ctgttggttg ccccctgaga gcgtgcgggc
     2821 ggatacctcg atccccggag tgcggacgtc gaactcctcg actgcgctcc gcgcctgagc
     2881 ccggatgtag tcgtagtcaa gaccgaggag tctggcatac gggcgcttcc actgattccc
     2941 caggagtatg ttctcggcaa ggtcgaagtc gagcacgagg ccccgcttct ggcgatcctg
     3001 tgggaccaac ccgagccctc gatctatgcg cccgcggact ccgtggctcg taatgtcttc
     3061 ttcaccgaaa aggatgcgcc ccgacgccga ctttcgcaga ccgatgatgc cttcttccag
     3121 ttcgatctgc ccgttgccgt ccactcccgc cacgccgagg atctccccct cgcggaccgc
     3181 gaacgagaca cccttcagcg cggtgagtcc cctctgatca cgcacgacga ggtcctctac
     3241 ccgcaggacc tccttccccg ctgcgtgctc ctccttctcc acctggagca cgacatcacg
     3301 gccgaccatc agttgcgcca actcccgttt ggtcgtggac ggggtgtcga gagtgccgac
     3361 cacgcgccca cgtcggatca cggtcacccg gtcgcttatc tctgtgacct cttcaagctt
     3421 gtgcgagatg aagaagattg aaaggccctg cttggtcagc gagcggaccg tgccgaacag
     3481 atcgtccact tcttgcggtg cgaggacggc agtcggctcg tcgagtatca gtatcttggc
     3541 cccggcgtac agtgccttca ggatctcggc tcgctgctgc atccccaccg aggcttccat
     3601 gaccttcctg tcgggatcga cctccaaacc gaacgtatcg gacagctccc tgaggcgtct
     3661 gcgcgcctcg cgcagatcca aggtccgggc tctcgtgtgg ggctcgtgcc caaggacgac
     3721 gttctccgcg atcgtaaact tctggatcaa catgaaatgc tggtgcacca tgccgatgcc
     3781 caaacccagc gccgtccgcg gtgacgggat gcgcacctgc cgcccctcga tctcgatggc
     3841 acccgcatca ggttcataca gcccgtagag gacgttcatc acggtggtct tacccgcgcc
     3901 gttctctccg agcagagcgt ggatctcccc tcgtcgaaga tcgaggtcga tcctgtcgtt
     3961 ggccacgacc ccaggaaaga acttagagat gcccgtcagc cggacgaccg tgtcggccga
     4021 ctcaggcccc ctagacgttt ggatgtgttc ttccacctgc ccccggccgt gggttcgcgg
     4081 gttctggtcg cgcctcagct gttcgccgcc attgtagtaa acaagcggtg gccgggtcgc
     4141 acggcgaccc ggccacctgc cggtcgggcg ctccggagga gcgccaccgg ccgaaactcg
     4201 acgtcagctc actagttgac caatgactcg tccacgatcg tggcgggcgc gagtgcgcct
     4261 gagaccatat cggccttgga cttctctatc aggtccttca acgtttgcgg aatgatggag
     4321 tcgaagtcat ggaaggagga caggcccact ccatcgttct tcaggtcgta cacgaagtta
     4381 cctgtgtcga agttgccttc cgaagcgtcc ttgacggcca cgaacgcccc catttggagg
     4441 ttcttcatgg ccgacacgag gatgacttcc gggagttccg gcgactgatc gacgtccacg
     4501 ccgattacga agtgaccggt gtccttggca gcctcgaata cacccagacc cgtctttccg
     4561 gcaacttgga agacgatatc ggccccgttc tcatacatgg ggagggctgc ttcttttcca
     4621 gcggtcggat cgatgaaggt atccgtgtag gtcaacaaga tcttgatatc aggatcgatc
     4681 gacttgacgg cgtctatgta gcccgcgatg tagcgatcta cggacgggac cttcatcccg
     4741 gccaccaagc cgacgacctt gtcagcgttg aggcgatcgg aagcacccgc ctccagagtc
     4801 atcgctccgg cgaccaggcc ggcaagatag cccacctgct gctccgcgta caggacctca
     4861 cgcacgttcg gaaggtctat cggattgtag tccttatcca cgagatcgat gtcgatcccc
     4921 gcgtacagcg tatcggggtg agctgtcgcc acctcgcgca gggcatcggt catgagcacg
     4981 cccacagcga caaccaacgg gttgccatcc tcagtcagtc gggtgaggtt cggcacgtag
     5041 tcggtctgct gttcagacac caagtacgac gtttcggcac caaggtcggc cttggcctgt
     5101 tccatgcccg cccacacgcc gtcgttgaac gacttgtcgc ccagcttgcc gacgtcggtg
     5161 accatcgccg ccttgtatgc cccgtccttg gaaccgctgt cacctccgca acctactacg
     5221 gtcaacgttc cgagcgccag gacgaccacc aggaacacaa ccaacttacg tctgctcatc
     5281 ccttcctcct cgattcgaga ctcgaccatg tccttcaggc gtcaagaaac acgacgggga
     5341 tgccgtcagc gccatcaaac gctccagcat cccccttctc tcagccgccc cgccctacac
     5401 cggcacctcc gaccgtaggg ccccgccggg ccgttcccga cgcccccatt cttacacgtt
     5461 cagagggcga tggctaccca tcgaaatcct ccgcttcgtc cggcgcctcc gtcgcgccgc
     5521 cgacagcttt caatgtctcc agcacccgct cggactcacc gggacccacc aggatctgac
     5581 gcgccttgct gccttcgtac ggccccacga tgccgcggcg ttccagcatg tcgatgagcc
     5641 tcccagccct cgtgtaaccg accctgaggc ggcgttgaag catcgacacc gacgcgctcc
     5701 cggtctccaa caccatctcc gccgcatcca gcagcagatc gtcgctgtcg ggatcgaagt
     5761 cgtcgtcgtc ccgcttcgag gtgggttgcg gcggcaggag aagctcctcc tggtacacgg
     5821 gttccgcttg gcggcggcag tgcgtgacca ggcgatggat ctcctcctcg gtgatgaagg
     5881 ctccctggac gcgctgcagg cggttcgaac cgagcggctt gaagagcatg tcgcccatac
     5941 ccagtagggc ctcggccccg ttgctgtcga gaatgacccg agagtccgtc tgactcgaca
     6001 ccgcgaaggc gatgcgcgag gggatgttgg ccttgatcat gcccgtgatg acgtccaccg
     6061 acggtctctg ggtcgcgacc accaggtgga tgcccacggc tcgggccttc tgcgccagcc
     6121 tgatgatgta ctcctccact tcgcccgggg agaccatcat gagatccgcc aactcgtcta
     6181 tcaccacgac gacgaacggc aagggccgct ccccgcgtct cgccctcgcc ttgttggctt
     6241 cctctaagtt gcgggccttc tcgagctcca taagctcgta ccggttctcc atctccttga
     6301 cgatgttgtg gagcacgccc gcggcgttct tcatgttggt taccacgggc accaggaggt
     6361 gggggacgta ctcgaagtgc gaaagctcca ccttcttggg gtcgataagg atcagcttca
     6421 ccttgtcggg cgaggcccgc agcaggatgg acgagatgat gcagttcacg cacccgctct
     6481 tccccgatcc agtggtcccc gcgatgagta ggtgaggcag ccgggcgaga tcggcgtgaa
     6541 cggacttccc ggagatgtcc ttgcccagcc acaccatcaa gggcccggcc gtgctcggga
     6601 agtccttgta gatgtctcca agtgtcacga aggtgggacg ctggttcggc acttccacgc
     6661 ccaccgcctg ctttccgggg atcggggcaa ggatgcggat ctccgtggat gccaaggcgt
     6721 aggcgagatc gtccttcaag cttgagacct tgctcacctt ggtgcctggc gccagctgca
     6781 gctcgaatct cgtcaccctc gggccgatga ccgtcccgat cacacgcgct tccactccga
     6841 agtgcgagag ggtcaggacg agttggtcgg cgatctcgat atcggctctg tcgctccgct
     6901 ccgccggagc ggcgctcttc ttcaggatct cgctgtcggg cagagcgtag tagggcgccg
     6961 ccgccactga agcccgccgc gcctccggct gaccgaggcc cggcaagatg ccctggacgg
     7021 gcacacctgt cgcctgcgcg cgttcggcgg tcgtggcgac gatctcgccc tccccgccgt
     7081 cgtcatcgac tgcctgcaga cgcccccggc ccggtgcatc gccggttccg gccgtgagca
     7141 gagacgactc cggaaggccg gacgaccccc cgaacgagct cgaagacgac cccagggggt
     7201 agatgtccgg gaaagcctgt gcgccgtcta tgaggtccgg acccgagcgg gtgtcggcga
     7261 cgcgctccag gggggtatcc caggaccgtc cggtcacggt ggtgtcggga tcggagggat
     7321 gtgaaggcca ctcgcctcca cccgaaacca caccgggctc ggtggcggca gtgcccggcg
     7381 cgccgccctg ccgcgccggt cgcgcggagc cgaacccagc aagataaggg tcgtcgagtg
     7441 ggccgcggtc gtcggtggtg acggcccgct cgcgcaggcg tcgcgtgcgc tcctgcgcgc
     7501 ccacctgtgc cgccttggcc gcgcgacccg tcccgagcac gaagcgcctg atcgtgacgc
     7561 cggtcaccat gctcaatcca ccgatcatca agagccagcc cacgatggcg a
//
LOCUS       DASRMJ010000152         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_11861, whole genome shotgun sequence.
ACCESSION   DASRMJ010000152 DASRMJ010000000
VERSION     DASRMJ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11861"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFD74_04265"
     CDS             complement(<1..252)
                     /locus_tag="VFD74_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZK48796.1"
                     /translation="MTIDPVCGKWIVERKAKAAAFHAGRIFYFCSEKHRDVFCADPEA
                     YAGKIKGGALRLGVMGAASGTFGPEVVKAAFELGAAAARR"
     gene            complement(249..1667)
                     /locus_tag="VFD74_04270"
     CDS             complement(249..1667)
                     /locus_tag="VFD74_04270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK48797.1"
                     /translation="MHLQVCGAAREVTGSCYLVEAGRTQFLVDCGLHQGGAKEEAENS
                     RPFPFDPREIDFMLLTHAHIDHSGLIPRLVKEGFGGPIYATSSTGDLAGIMLLDSAHI
                     QEMEAEWQDRKGRRAGRSTKAPLYTQKDAEIALRQFRPVEYEKDIHPSAEVTVRFRDA
                     GHILGSAALEIRLTTAAGSSKVVFSGDVGQRDRPILRDPEVITEADYLIMESTYGDRL
                     HEGNHGDPVEELRAIVEEAHRTGGNVIIPAFAVGRTQELVYYLREICVREICATECHG
                     VQIFVDSPMATKATAVFRAHRAEFDKEATDLISENGSIFNFPELHYTLSADESKALND
                     VKGAIIISASGMADAGRIKHHLKHNLWKPEAHVVFVGYQAQGTLGRRLLEGEKRVRLF
                     GEEIVVRAKIHDISGLSAHADREQLIAWASSFKPAPFTILTHGEPKAALSLAAALKER
                     LGFEVVVPELFETIELEERVQA"
     gene            2029..>2391
                     /locus_tag="VFD74_04275"
     CDS             2029..>2391
                     /locus_tag="VFD74_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48798.1"
                     /translation="MSNKLLVKGAALIAAGALGLVGLGALGPVFGPGTASADGNAPSW
                     MRPSAVSPVQSAGTSGANVEDRVTEMLRDHMGITGDQAAEWAGTMADMMRSVHGDQTE
                     AMLDYCDENGGPQGMMGSG"
BASE COUNT          410 a          806 c          746 g          429 t
ORIGIN      
        1 gcgccgcgcc gctgccgctc ccagctcgaa ggcggccttc accacctccg gtccgaaggt
       61 gcccgaagcg gcgcccatca cgccgagtcg cagcgctccg cccttgatct tgccggcgta
      121 ggcctcggga tcggcgcaga acacatcccg atgcttctcg gagcagaagt agaatatgcg
      181 cccggcgtgg aaggcggcgg ccttcgcctt cctctccacg atccacttgc cgcacacggg
      241 gtcgatcgtc acgcctgcac cctctcctcg agctctatgg tctcgaacag ttcgggcacg
      301 accacctcga accccagccg ctctttcagg gccgccgcaa gtgacaaggc cgccttcggt
      361 tcgccgtgag tgagtatggt gaacggcgcc ggtttgaagc tcgaagccca ggcgatcaac
      421 tgctcacggt cggcgtgagc ggacaggccg ctgatgtcgt ggatcttggc ccgcacgacg
      481 atctcctcgc cgaacagcct caccctcttc tcgccttcga gcagccgacg gcccagagtg
      541 ccttgggctt ggtatcccac gaagacgaca tgcgcctccg gcttccacag gttgtgtttg
      601 agatggtgct tgatccggcc ggcatccgcc atgccgctcg cggagatgat gatcgctccc
      661 ttgacatcgt tgagcgcctt ggattcgtcg gccgagaggg tgtagtgcaa ttcggggaag
      721 ttgaagatcg acccgttctc gctgatgaga tcggtggctt ccttgtcgaa ctccgcccgg
      781 tgggcgcgaa agaccgccgt cgccttcgtc gccatgggac tgtcgacgaa tatctgcacg
      841 ccgtgacact ccgtggcgca gatctctctc acgcagatct ctctcagata gtagacgagt
      901 tcttgcgtcc tccctacggc aaaggcgggg atgatgacgt tgccgccggt gcggtgggcc
      961 tcctcgacta tggcccgcaa ctcctcgacc gggtcgccgt ggttgccttc gtgcagtcga
     1021 tccccgtagg tggattccat aatgaggtag tcggcttcgg tgatcacctc cggatcgcgc
     1081 aggatcggac ggtcccgttg tcccacgtcc ccgctgaaca cgaccttcga ggagcccgcc
     1141 gccgtggtca gccggatctc cagagcagcc gacccgagga tgtgacccgc gtcgcggaat
     1201 cgcactgtga cctcggccga gggatggata tccttctcat attccacggg ccggaactgg
     1261 cggagggcga tctcagcatc cttctgggtg tagaggggtg ccttcgtgct ccgcccggca
     1321 cggcggccct tgcgatcctg ccactccgct tccatctcct ggatgtgggc cgagtcgagc
     1381 agcatgatgc cggctagatc gccggtggac gacgtcgcgt agatcggtcc gccgaagccc
     1441 tccttgacca gtctggggat gagacccgag tggtctatgt gcgcgtgggt gagcaacatg
     1501 aaatcgatct cacgggggtc gaacgggaag gggcgactgt tctcggcctc ctccttcgct
     1561 cccccttggt ggagtccgca gtccaccagg aactgggtgc gccccgcttc caccaggtag
     1621 caggagccgg tgacctcgcg cgccgcgcca cacacttgca gatgcataca agacctccgc
     1681 aactcgccgt cgccgccgcc gcccgcccga cgcctggacc ctcaccggga acggtgcctg
     1741 accggttcgc atcaggatac atcgcggacg accgatacgt cgccccgcgg gcccgcatga
     1801 tggtccccgg gcgcgcccga accaacggcg cctgccggag cctacaacgc ccgccgaaac
     1861 cgacgaagcc tgcccgagcc acgacgccct cggataccct ccggcgattc tccccccgca
     1921 acctttacgt gagctttgca ttctcttgcg acactctcaa cacagatgca cgacactagg
     1981 aacgtagcaa acacatccga ctcgatcgga tctgccaaag gagatgcgat gtcgaacaag
     2041 ttgttggtca aaggagcggc actgatcgcc gcgggggccc tgggtctcgt cggcctcggg
     2101 gccctcggtc cggtattcgg ccccggaacc gcctccgccg acggaaacgc cccgagctgg
     2161 atgaggccct cggcggtaag ccccgtgcaa agcgcgggca cttccggtgc gaacgtcgag
     2221 gatcgcgtga ccgagatgtt gcgcgatcac atgggcatca ccggcgacca ggccgcggaa
     2281 tgggcgggca cgatggcgga catgatgcgt tcggtgcacg gcgaccagac cgaagcgatg
     2341 ctcgactact gcgatgagaa cggtgggccg caagggatga tgggtagtgg c
//
LOCUS       DASRMJ010000153         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_48178, whole genome shotgun sequence.
ACCESSION   DASRMJ010000153 DASRMJ010000000
VERSION     DASRMJ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48178"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..531
                     /locus_tag="VFD74_04280"
     CDS             31..531
                     /locus_tag="VFD74_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZK48799.1"
                     /translation="MTTSTERLDSMVRDATFNIDRALGALRERGRRSTKLKRAVLRSF
                     MGGDCALTAEEIADRIGVGTELSPLYRCLSSLEEAGVLTHLYLGDGGRRYDLADEFGG
                     HHHHLVCEKCSRIARIDGCILGGDAAAEAVAEAAAQGYVMSDHEIVLRGVCAGCRDVG
                     DAADGA"
     gene            521..1588
                     /locus_tag="VFD74_04285"
     CDS             521..1588
                     /locus_tag="VFD74_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HZK48800.1"
                     /translation="MAHDSLGPPRPARNGVHTRRRALVGAVRAAAVVLVLAASLAPAA
                     GCGGADGAGGTGGAAGAAGSGGGGGGGEGAARLLVVTSVPVIYSLTANVAGDAVRLEN
                     LLPPGSSPHQVTFTPSQARLISDADVLIINGANLEPWVDDLVASAGNSDLQVVVASDG
                     VEFLRPDEALPLPSSGSGEQTSPGDVDPHVWLDVSNARLMVGNIAEALCRVDPAGAAD
                     YRRRAATYTATLDALDSEVRAALAPLSNRRFVSFHSAFQYYARAYGLEQVAVIEEFPG
                     KEPSPQYLAGLVRLIGELKVPAVFSEPQFSARPAEALARETGVSIYEVDPEGAAISPT
                     MYEDLIRKDTEVFVTALGGAQ"
     gene            1615..2496
                     /locus_tag="VFD74_04290"
     CDS             1615..2496
                     /locus_tag="VFD74_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HZK48801.1"
                     /translation="MSGEKERRAPDDMRTSDLYPDAAVLVEDLTLVIGDTPIFERVSF
                     VVNRGETAMIIGPNGAGKTMLVRTMLDLLPPQTGTVTLLGKDSRCLGSTRDRIGYMPQ
                     RLDFDRTFPITVFEIMLLRLRKSGFWLNRKAKRKTVLDALTRVHAERLVDRRIGRLSG
                     GELQRVLLAYALLLDPDLLILDEPAAGVDVAGEDTFYELIHHIRTERHLTVVMVSHDL
                     EVVFKYADQVLCLNRELLCQGVPGAVLRPEILEKTYGSLAASYRHHHEHPDEHDLEHD
                     REHDREHDPGAGGTERR"
     gene            2496..3305
                     /locus_tag="VFD74_04295"
     CDS             2496..3305
                     /locus_tag="VFD74_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZK48802.1"
                     /translation="MTDLLSFLQYPFMQRALLAVVTVGFLCAVVGVYVTLRSMSFFSD
                     AISHSALAGIALGVLLGIDPVVAAVVFCVVVAAGITFLTVRTDLTSDTVIGVFFSGSM
                     AVGVLLIGLQKGYQTDLLSYLFGDVLAVNNLDVALSLGLAAAVVASLFRRSTLLIKIA
                     FNRDLAAVEGANVVAWDYAFMMLLALTVAVSVKIVGIVLVSALIIVPAAAARNIASDF
                     RRLMGIAIVIGLTGATVGLVASYYLDTASGPTMVMVVILIFIVTLVAGRRR"
     gene            complement(3411..3839)
                     /locus_tag="VFD74_04300"
     CDS             complement(3411..3839)
                     /locus_tag="VFD74_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HZK48803.1"
                     /translation="MDESAARAPRVDPLEYQSVVRLRLGGHDAHYGGGLVDGARIVGL
                     FGDVATELLIAFDGDEGLFVAYDSIEFTAPVFAGDYVEARGRITRVGRTSRTMEFEAV
                     KVIAARPGVSASAADLLAEPQVVCRATGTCVTPAECQRLS"
     gene            complement(3832..>4053)
                     /locus_tag="VFD74_04305"
     CDS             complement(3832..>4053)
                     /locus_tag="VFD74_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48804.1"
                     /translation="GRGGGVAYSAAVLRAVVGATMGVAATVAAMAAATVAAEPGPPRG
                     LSTLALRDTLSGARSSPGRDHSSGRTWHG"
BASE COUNT          616 a         1413 c         1349 g          675 t
ORIGIN      
        1 gcgtagtatt gttgctctta tcgtcatcga atgaccacaa gcacggagcg actcgatagc
       61 atggtccgcg acgccacctt caacatcgat cgggcactcg gcgccctgcg cgagcgcggc
      121 cgccgttcga cgaagctcaa gcgcgcggtg ctgcgcagct tcatgggcgg cgattgcgct
      181 ctcacggccg aggagatcgc ggatcggata ggcgtgggaa cggaactctc tcccttgtac
      241 cggtgcctgt catcgctgga ggaggccggg gtcctcaccc acctctatct gggtgacggg
      301 gggcgtcggt acgatctcgc cgacgagttc ggcggccacc accaccacct tgtctgcgag
      361 aagtgctcgc gcatcgcacg catcgacggg tgcatcctcg gcggggacgc ggcggcggag
      421 gctgtggccg aggctgcggc gcagggctac gtgatgagcg accacgagat cgtgctgagg
      481 ggagtctgcg ccgggtgtcg agacgtcgga gatgccgccg atggcgcatg actcgctcgg
      541 cccgccgagg ccggcgcgca acggggtcca cacgcgacgc cgagccctgg tgggcgccgt
      601 ccgagccgcg gcggtggtgc tcgtgctcgc tgcttccctt gcccccgcag ccggctgcgg
      661 gggcgctgac ggcgcgggcg ggacaggtgg tgcggcgggc gccgccggct ccggcggagg
      721 aggtggcggc ggcgaaggcg ccgcgcgcct tctggtggtc acctctgtcc ccgtgatcta
      781 ctcgttgacg gccaacgtgg ccggcgacgc cgtgcggctg gagaacctgc tgcctccggg
      841 atcctctccc caccaggtga ccttcacgcc ctcccaggct cgtctcataa gcgacgccga
      901 cgtcctgatt atcaacggcg cgaacctgga gccctgggtg gacgatctcg tggcttcggc
      961 gggcaactcc gatctccagg tggtcgtggc ttccgacggt gtcgagttcc tgcgcccgga
     1021 cgaggccctg ccgctgccgt cttccgggtc gggcgagcag acctcccccg gcgacgtgga
     1081 ccctcacgtg tggctcgacg tctcgaacgc ccgcctcatg gtcgggaaca tcgccgaagc
     1141 gctctgccgc gtggatccgg ccggcgccgc cgactaccgg agacgcgcgg cgacctacac
     1201 cgcgactctt gacgcgctcg actccgaggt gcgggccgcc ctcgcgcccc tctccaaccg
     1261 ccgcttcgtg agcttccaca gtgcctttca gtactacgcc cgcgcttatg gtctggagca
     1321 ggtggcggtg atcgaggagt tccccggcaa ggagccctct ccgcagtatc tggcgggctt
     1381 ggtgcgcctg ataggcgagt tgaaggtgcc ggccgtcttc tccgaaccgc agttctcggc
     1441 gcgacccgcc gaggcgctcg cccgcgagac aggcgtgagc atctacgagg tcgatccgga
     1501 aggcgccgcc atctccccga ccatgtatga agaccttatc cggaaggaca cggaggtgtt
     1561 cgtcaccgct ctcggaggcg cgcagtgacc gcaggagcag aggagccgcc cgcggtgagc
     1621 ggcgaaaagg agcgccgagc gccggacgac atgcgcacga gcgacctcta ccccgacgcg
     1681 gcggtgcttg tggaggacct cacgctcgtc attggagaca ctcccatctt cgagcgagtg
     1741 tccttcgtgg tgaaccgagg cgagaccgcc atgatcatcg gcccgaacgg cgccggcaag
     1801 accatgctcg tgcgcaccat gttggacctc ctgccgccac agacgggcac cgtcacgctc
     1861 ttgggcaagg acagtcgctg tctcggaagc acccgcgatc gcatcgggta catgccacag
     1921 cgcctggatt tcgaccgcac cttccccatc accgtcttcg agatcatgct gttgcgactg
     1981 aggaagagcg gattctggct caaccgcaag gcgaagcgga agacggtgct cgatgctctc
     2041 acccgtgttc atgcggagcg cctcgtcgat cgacgcatcg ggcgtctctc cggtggagaa
     2101 ctgcagcggg tcctgttggc ctatgccttg ctcctcgatc cagacctcct gatcctggac
     2161 gagcccgccg cgggcgtcga cgtggcggga gaggacacgt tctacgagtt gatccaccac
     2221 atccgcaccg agcgccacct gacggtggtc atggtgtcgc acgacctaga ggtggtcttc
     2281 aagtacgccg accaggtgct atgcctaaac cgagaactgc tctgccaggg cgtgccgggc
     2341 gccgtcctgc gacccgagat cctggagaag acctacggtt cgctcgccgc ctcctatcgt
     2401 caccaccacg agcaccccga cgagcacgac cttgagcacg accgcgagca cgaccgcgag
     2461 cacgacccag gcgcgggcgg gacggaacgg cgctgatgac cgacctgctc tcgttcctcc
     2521 agtatccctt catgcagcgc gcgctgctcg ctgtagtgac cgtgggattc ctctgcgcgg
     2581 tggtgggcgt ctacgtgacc ttgaggtcga tgtctttctt ctcggacgcc atctcgcatt
     2641 cggccctggc gggcatcgct ctcggagtgc tcctggggat cgacccggtc gttgcggccg
     2701 tcgtcttctg cgtcgtggtc gcggcgggca tcacgttcct caccgtgcgt accgatttga
     2761 cctcggacac cgtcatcgga gtgttcttct cggggtcgat ggccgtgggc gtgctcctca
     2821 tcgggctgca gaagggctac cagaccgacc tgctctcgta tctgttcggc gacgtcttgg
     2881 ccgtgaacaa cctcgacgtg gccttgagcc ttggtctggc ggccgccgtt gtggcttcct
     2941 tgttcaggcg ctccacgctg ctgatcaaga tcgcgttcaa ccgggacctc gcggcggtcg
     3001 aaggcgcgaa cgtggtggcg tgggattacg ccttcatgat gctgctcgcc ctcaccgtcg
     3061 cggtgagcgt caagatcgtg gggatcgtgc tggtgagcgc cttgatcatc gtcccggcgg
     3121 cggcggcccg caacatcgcc tcagacttca ggcgactgat gggcatcgcc atcgtcatcg
     3181 gactcaccgg cgccaccgtc ggactcgtcg ccagctacta cctggacacg gcctccggac
     3241 ccacgatggt gatggtggtc atactcatct tcatcgtgac cctggtggcc gggcggcgga
     3301 ggtaggaagc ccacgtcggg cacgcccggt gccgcgcccg cgcgccgcgg ggccggcggc
     3361 accagccggc ggccgccacg actgccacgt cttcctccta ccgcgagcgc ctacgacaga
     3421 cgttggcact ccgccggggt gacgcaggtg ccggtggccc ggcacacgac ctggggctcg
     3481 gcgagcaggt ccgcggccga agccgacacg ccgggccgag ccgcgatcac cttcaccgcc
     3541 tcgaactcca tggtccgcga cgtcctcccc acccgcgtga tgcgaccccg agcctcgacg
     3601 tagtcgcctg cgaacacggg cgccgtaaac tcgatggagt cgtacgcgac gaagaggcct
     3661 tcgtctccgt cgaaggcgat gagcaactcg gttgccacgt cgccgaacag ccccacgatg
     3721 cgggctccgt ccaccagtcc gccgccgtag tgcgcatcgt gcccccccag tcgcagccgg
     3781 accaccgatt ggtactccaa cggatccacg cgcggagccc gcgccgccga ctcatccatg
     3841 ccaagtcctc ccagaagagt ggtcgcgccc gggcgaagac cgggcgccgc tgagagtatc
     3901 ccggagggcc agcgtcgaca agccgcgggg cgggcccggc tccgcggcga ccgtggcggc
     3961 ggccatggca gcgaccgtgg cggcgacgcc catggtggct cccacgacgg cgcgcagcac
     4021 cgccgccgag tacgcaacac cgccgccacg tcc
//
LOCUS       DASRMJ010000154         6372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_156325, whole genome shotgun sequence.
ACCESSION   DASRMJ010000154 DASRMJ010000000
VERSION     DASRMJ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156325"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="VFD74_04310"
     CDS             <1..154
                     /locus_tag="VFD74_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZK48805.1"
                     /translation="DRLQRETTGRVVPDSFTHGTSEQRARWFRLGLETGDVSRGDTFS
                     VPENRL"
     gene            complement(151..1158)
                     /locus_tag="VFD74_04315"
     CDS             complement(151..1158)
                     /locus_tag="VFD74_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK48806.1"
                     /translation="MSPDPEAASGAAGLSRAAGVSRASGLPEAAALPEAAALPEAAAL
                     PDAADPARDDTLPVERWLAAAGARISRRSYTDEPVSGPAADALDRLCRSFAPFEGART
                     VLVRHAPPQMFTGWLLHVYGKVSSPSALVFVGDQRVPGAAARAGYTGEAAVLEATALG
                     FATCWIGGGVRRSVVEEFLKLEPHEHVYSISALGYTSGEPSLGERATAGLVKARKRKA
                     LETIAPGIQAWPPQVRVGVEAARLAPSATNRQPWRFTFAPAAQDPGSSNGPVGPGDKV
                     RLSFERADTPVISKSLDCGIAMLHFELGVRAAGVAGSWALLDGNDVATWTFARERGAS
                     S"
     gene            complement(1155..3092)
                     /gene="typA"
                     /locus_tag="VFD74_04320"
     CDS             complement(1155..3092)
                     /gene="typA"
                     /locus_tag="VFD74_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZK48807.1"
                     /translation="MPISAAQIRNLAIIAHIDHGKTTLIDSIFRAAHTFRDNARIEER
                     VMDNNDLERERGITIRAKHCAVEWSGYRINLVDTPGHADFSGEVERVLSMVDSVLLLV
                     DANEGPMPQTRYVLARALSLGYKPIVIVNKVDRPNANARKALEQTFDLFIELGATDAQ
                     ADFPVLYGSGLEGWLVRDLDAGPREGMDDLFETIVSYVPAPLVDVDAPFRMQVSTLAW
                     SDYIGRIGGGRVLSGTHRRGEELVRSRVGAAEVEPAVGSSGATVVGRSTAISENSRSS
                     HLFGTTGLERVEMDEVTAGDIVWIAGPEELNIGDTLSSPEFVDPAMPPLDVEEPTVSM
                     FFLVNTGPFAGEQGRAATLRQIRDRLEREARVNVALKVEDLGRADGLKVSGRGELHLA
                     ILIEEMRREGIELCVSRPEVITRRDDDGVLLEPYEDLVVDVPGEYQGAVIEKLARRKG
                     ELKGMHTEGTGMVRLEFAIPTRGLIGYRTEFLTDTRGYGVMAARVSDYRPWAGEVSSR
                     NRGSLVSMEIGTATGYSLENLQTRGTLFLSPGERVYAGMIVGEHSRPEDMACNPTKMK
                     KLGNYRDNKDIDVGLKVPRRLGLDQSLEWIAADELVEVTPGFIRMRKAVLNAEQRKRD
                     EKRSATARAEAASDSAGSAAA"
     gene            3314..5467
                     /locus_tag="VFD74_04325"
     CDS             3314..5467
                     /locus_tag="VFD74_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HZK48808.1"
                     /translation="MDALLTPLTNIVAAELAVRSGQVEAVADLLAGGATVPFIARYRK
                     EATGSLDEVQIATVRDRLARLEELGKRRESILSSLRERELLTGELEAAVLGAATMAEL
                     EDHYLRFRPKRRTRAAAARERGLEPLAALLLAAAAAGDAHFDPSAAASAYVDPDKDVP
                     GVDEALAGARDIIAEAASEDADVRKRMRALYVREAALRSRVAKGKEEAGAKFRDYFDW
                     EESAERAPSHRVLALLRGEAEGFLRVGVEVPEERAAAIVDGRFVRGSGAAADEVRAAL
                     RDSLKRLLSPSMESEMRAELKARADEEAIRIFASNLAELLLAPPLGGRTVLAIDPGFR
                     TGCKVTVLDPQGKLLHNETVQPHQSKGARAQAGETIRTLAAQFGVEAVAVGNGTAGRE
                     TETFVRELDLGRDALVVMVNESGASIYSASEVAREELPDHDLTVRGSVSIGRRLQDPL
                     AELVKIDPKSIGVGQYQHDVDQAALRRSLDDVVASAVNRVGVEVNTASRSLLSHVSGL
                     GPQLAANIVAYRDQEGPFRSRVQLKKVPRLGPKAFEQAAGFLRIRGGADPLDASAVHP
                     ESYGVVKAMAADLGVKVGDLLRDAELRARVELSRYATEKVGLPTLRDIMAELEKPGRD
                     PRQAFEVFRFAEGVESLDDLEEGMRLPGIVTNVTAFGAFVDIGVHQDGLVHVSQLADR
                     FVRDPADVVAVQQRVMVTVLGVDLQRRRISLSMRQ"
     gene            5472..6176
                     /locus_tag="VFD74_04330"
     CDS             5472..6176
                     /locus_tag="VFD74_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HZK48809.1"
                     /translation="MRYEWLFLDADGTLFDYDSAESNALRAAFLESGLAYGDEALATY
                     RAFNRAVWVEFENGTMSAATLRVRRFEQLFAALGLEADAAAFSPRYLEHLSRGTELLE
                     GAERLVRTLRGRVGMALITNGLKDVQRPRLRGSALHGLFDHVIVSDEIGVAKPSAGFF
                     DIAFDAAGAPAKEKVLVIGDSLTSDIQGGIDYGVDTCWFNPRGASADPRYPATYEIRS
                     LPELYPLVGLAPDPAG"
BASE COUNT         1010 a         2113 c         2223 g         1026 t
ORIGIN      
        1 cgaccgcctg cagcgggaaa ccacgggccg ggttgttcct gactccttca cgcacggtac
       61 gtccgagcag agggcgcggt ggttccgtct agggctcgag accggcgacg tatcccgggg
      121 agacaccttc tcggttccgg agaacaggct ctagctggag gcgcctcgtt cgcgggcgaa
      181 ggtccaggtg gccacgtcgt tgccgtcgag gagcgcccac gaaccggcga cccccgcggc
      241 gcggacgccc agctcgaagt gcagcatggc gatgccgcag tccagactct tcgagatgac
      301 cggcgtgtcg gcgcgctcga agctgaggcg caccttgtcc cccggcccca cggggccgtt
      361 cgacgaaccg gggtcttgag cagcgggcgc gaaggtgaag cgccacggct ggcggttcgt
      421 cgctgaggga gccaggcgcg cggcttcgac cccgacacgg acctgaggcg gccacgcctg
      481 tatccccgga gcgatagtct cgagcgcctt cctcttccgc gctttgacca ggcctgcggt
      541 cgcccgttcg ccgagcgaag gttctcccga agtgtagcca agagcggaga tcgagtacac
      601 gtgttcgtgg ggttcgagct tcaagaactc ctcgacgacg ctgcggcgga ccccgccgcc
      661 gatccagcaa gtggcgaagc cgagggcggt ggcctccaag acggcggcct ccccggtgta
      721 cccggcccgc gcggcggcgc cgggaaccct ctgatcgccg acgaagacca gggccgacgg
      781 agaagacacc ttcccgtaga cgtgcagcag ccatcccgtg aacatctgcg ggggagcgtg
      841 acgcaccagc acggtgcggg cgccctcaaa gggggcgaag gagcggcaga gcctgtccag
      901 agcgtcggcg gcgggtccgg acacgggctc gtcggtgtag ctgcgtcgcg agatccgggc
      961 gcccgccgcg gcgagccaac gttctaccgg caacgtgtcg tcgcgggcag gatcggcggc
     1021 gtcaggaaga gccgcggcct caggaagagc cgcggcctca ggaagagccg cggcctcagg
     1081 aagtcccgag gcgcgcgaga ctccggcggc gcgactgagt ccggcggcgc cgctcgcggc
     1141 ttccggatca ggactcacgc cgccgccgac cccgcagagt cggacgcggc ttctgcccgc
     1201 gctgtggctg aacgcttctc gtcgcgcttg cgttgttcgg cgttgaggac ggccttccgc
     1261 atgcggatga agccgggcgt gacctctacc agttcgtcgg ccgcgatcca ctccaggctc
     1321 tggtcgaggc cgagtcttcg aggcaccttc aaaccgacgt cgatgtcctt gttgtctcgg
     1381 tagttgccga gcttcttcat cttggtgggg ttgcaggcca tgtcctccgg ccgcgaatgt
     1441 tcgcccacga tcatgcccgc gtaaacccgc tctcccggag agaggaagag ggtgccccga
     1501 gtctgaaggt tctccaggct gtagccggtg gcggtgccga tctccatgct gacgagggag
     1561 cctcggttgc gcgaagagac ctcgcccgcc caaggacggt aatcgctcac tcgggctgcc
     1621 attacgccgt atccacgggt gtcggtgagg aactcagtcc ggtagccgat gagaccgcgc
     1681 gtggggatgg cgaactccag cctcaccatg ccggtgccct ccgtatgcat ccccttgagc
     1741 tcgcccttgc gtcgggccag cttctcgatg actgcgccct gatactcgcc agggacgtct
     1801 acgaccaggt cttcgtacgg ctccaagagg acgccgtcgt catctctcct ggtgatcact
     1861 tccggcctcg atacgcagag ttcgattccc tcgcgtcgca tctcctctat gaggatggcg
     1921 aggtgcaatt cgccgcgccc cgacaccttg aggccgtcgg cccggccgag atcctctacc
     1981 ttgagggcca cgttcacccg cgcttcacgt tcgaggcggt cgcggatctg gcgcagtgtg
     2041 gcggcgcgtc cctgctctcc cgcgaaaggc cccgtgttga ccaggaagaa catcgagacc
     2101 gtaggctcct cgacgtcgag cgggggcatg gcggggtcca caaactcggg ggaactgagt
     2161 gtgtcgccga tattcagctc ctccggcccc gcgatccaga cgatgtctcc ggcggtgacc
     2221 tcatccatct ccacccgctc caggccggtg gtgccgaaca ggtggctcga gcgcgagttc
     2281 tcggagatgg cggtgctgcg tccgacgacg gtcgctccgg aggagccgac cgcaggctcc
     2341 acctcagcgg cgcccaccct actgcggacc aactcctccc cccgccggtg cgtgccagag
     2401 aggacccgtc cgccgccgat acgcccgatg tagtcgctcc aggcgagcgt ggacacctgc
     2461 atgcggaacg gcgcatcgac gtctaccagc ggagctggga cgtacgagac gatggtctcg
     2521 aagaggtcgt ccatgccttc ccgcggtccg gcgtcaaggt cgcgcaccag ccagccctcg
     2581 agtccggagc cgtagagcac ggggaagtcg gcctgtgcgt cagtggcgcc caactcgatg
     2641 aagagatcga aggtctgctc gagagccttg cgggcgttcg cgttcgggcg gtccaccttg
     2701 ttcacgatca cgatcggctt gtagccaagg ctcagggcac gggcgagcac gtagcgggtc
     2761 tgcggcatgg gaccctcgtt ggcgtccacc aacaggagga cggagtcgac catggacaac
     2821 acgcgctcga cttccccgga gaagtcggcg tgacccgggg tatccacgag gttgatgcga
     2881 tagccgctcc actcgactgc gcagtgcttg gcacggatgg tgatgcctcg ttcgcgctca
     2941 aggtcgttgt tgtccatgac ccgttcttcg atgcgagcgt tgtcgcggaa ggtgtgggcg
     3001 gcgcggaata tggagtcgat cagcgtggtc ttaccgtgat cgatgtgggc gatgatcgcg
     3061 agattacgga tctgagcagc agaaatgggc atggtgattc ttccgaggcc tctctcttca
     3121 aggggggtgg accggcgtag tccgggtgga gattgtacac taccgcggtg gttagcgact
     3181 ttcgacccgg tgacggcggc ccgcgcggcc gggaaatgcc ggccggccgg cggcacgcgc
     3241 agcccggaaa tgccggccgg ccggcggagt cgccttgcag ccgcaccgca catctgcgaa
     3301 cctacctcgg cctatggacg cgctcctgac accactgacc aatatcgtgg ccgccgaact
     3361 cgccgtgcgg tcggggcagg tggaggccgt ggcggatctg ctggccggcg gcgccaccgt
     3421 gcccttcatc gcgcgctacc gcaaggaggc caccgggtcg ctcgacgagg tgcagatcgc
     3481 gaccgtgcga gaccgcctcg cccgtctcga ggaactcggg aagcgacggg agagcatcct
     3541 gtcctccctg cgcgaacggg agctgctcac gggtgaactc gaggccgccg tgctcggggc
     3601 ggccaccatg gccgaactcg aagaccacta tctgcggttc cgcccgaagc gccgcacccg
     3661 ggcggccgca gctcgggaac gcgggctcga acccttggcc gcgctgctcc tggcggcggc
     3721 cgccgccggt gacgcccact tcgatccttc ggcggccgcc tccgcctacg tggaccccga
     3781 caaggacgtg cctggtgtcg acgaagctct ggccggcgcg cgcgacatca tcgcggaggc
     3841 cgcaagcgag gacgccgacg tgcggaagcg catgcgcgcc ctctacgtga gggaagccgc
     3901 cctgcggagc cgggtggcga aggggaagga ggaggccggc gccaagttcc gcgactactt
     3961 cgactgggag gagtcggcgg agcgcgctcc gtcgcaccgg gtgttggccc tcctgcgcgg
     4021 cgaagcggag gggttccttc gggtgggcgt ggaagtgccc gaggagcgcg ccgccgccat
     4081 cgtggacggc cgcttcgtgc gcgggagcgg agccgccgcc gacgaggtgc gcgcggcgct
     4141 ccgcgacagc ctcaagcgac tcctgagccc gagcatggag agcgagatga gggcggagtt
     4201 gaaggcccgc gcggacgagg aggccatccg catcttcgcg tcgaacctgg ccgagttgct
     4261 gttggccccg ccgctgggcg gccgcaccgt gctggcgatc gacccgggct tccgcaccgg
     4321 ctgcaaggtc acagtgctcg atccccaggg gaagcttctg cacaacgaga cggtgcagcc
     4381 tcaccagtcg aagggggcga gagcgcaggc cggggagacg atccgaacct tggcggcgca
     4441 attcggagtg gaggcggtgg ccgtgggcaa cggcaccgcc gggcgcgaga ccgagacctt
     4501 cgtgcgggag ctggatctgg gccgcgacgc tctggtggtc atggtgaacg agagcggcgc
     4561 ctccatctac tcggcatcgg aagtggcgcg cgaggagctg cccgaccacg acctgaccgt
     4621 gcgcggttcg gtctctatcg gtcgtcgcct gcaagacccg ctcgccgaac tggtgaagat
     4681 agaccccaag tccatcggtg tgggccagta ccagcacgac gtcgaccagg ccgccttgcg
     4741 acggagcctc gacgacgtgg tggcgagtgc cgtgaaccgc gtgggggtgg aggtgaacac
     4801 ggcgagccgt tcgctgctca gccacgtgtc cggtctgggg ccgcagttgg ccgccaacat
     4861 cgtggcgtac cgtgaccaag aaggtccttt ccgctcgcgg gtgcagctca agaaggtgcc
     4921 gcgcctgggc cccaaggcgt tcgagcaggc cgccgggttc ctccgtatcc ggggcggcgc
     4981 cgacccgctc gatgcgagtg cggtgcaccc cgagagctat ggcgtggtca aggccatggc
     5041 cgccgacctc ggcgtgaagg tgggcgacct gctccgtgac gcggagcttc gcgcccgcgt
     5101 cgaactctcg cgctacgcca ccgagaaggt gggtctgccc accctgcgcg acatcatggc
     5161 cgaactggag aagcccggcc gcgatccccg gcaggcgttc gaggtgttcc gtttcgccga
     5221 gggtgtcgag tcgctcgacg accttgaaga ggggatgcgt ctgcccggga tcgtcaccaa
     5281 cgtgacggct ttcggggctt tcgtcgacat cggcgtgcac caggacggcc tcgtgcacgt
     5341 gagtcaattg gccgaccgct tcgtgcggga cccggccgac gtggtggcgg ttcaacagcg
     5401 ggtcatggtg acggtgctcg gggtcgacct ccagcggcgc cgcatctcgc tcagcatgcg
     5461 acaataggcg catgagatac gagtggcttt tcctcgacgc cgacggcacc ctgttcgact
     5521 acgattcggc cgagtcgaac gctctccgcg ccgcgttcct cgagtccgga ctcgcgtacg
     5581 gtgacgaggc tttggccacc taccgcgcct tcaaccgcgc tgtctgggta gagttcgaga
     5641 acggcacgat gagcgccgcg accctgcggg tgcgtcgctt cgagcaacta ttcgcggccc
     5701 tgggtcttga ggccgacgcc gcggccttca gcccccgcta cctcgagcac ctgtctcgag
     5761 ggaccgaact cctcgaagga gcggaacgcc tcgtgcgcac cctccgtggg cgcgtcggca
     5821 tggccctcat cacgaacggt ctgaaggacg tgcagagacc ccgtctccgt ggttcggcgc
     5881 tgcacgggct gttcgatcac gtgatcgtct ccgacgagat cggggtggcc aaaccctcgg
     5941 ccggcttctt cgacatcgct ttcgacgcgg cgggcgcacc ggcgaaggag aaggtgttgg
     6001 tgatcggcga cagtctcacc tccgacatcc aagggggcat cgactacggg gtggacacgt
     6061 gttggttcaa tcctcgcgga gcgtcggccg atccgcgcta cccggcgacc tacgagatcc
     6121 gtagtctgcc cgagctctat ccgctggtgg gtctcgcacc cgacccggcg gggtgagcgg
     6181 gtacgtgcca aggcgatgcc gagctcgatg aagactcccc atccgcaacc ccacgacgtc
     6241 cttgatcagt cttcgacgcc tctggccgac gccatcgagg agtaccggaa gcgggggatg
     6301 gccgcctacc acacgcccgg catccctctg ctcggaccgg gggaggaggt ctcggaggtg
     6361 atagtcgagt at
//
LOCUS       DASRMJ010000155         5740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_454451, whole genome shotgun sequence.
ACCESSION   DASRMJ010000155 DASRMJ010000000
VERSION     DASRMJ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454451"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFD74_04335"
     CDS             complement(<1..571)
                     /locus_tag="VFD74_04335"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HZK48810.1"
                     /translation="MEGLWVVLALALGAIGIAAGYLLRKFVGEAKITSAEQEASKILE
                     TAQRQAEAEKREALVGAKDELHKLRTDTETELRDRRNELSQLEKRVLQRDESLDEKLK
                     EVGRREQAVADREIHYRERLEEVEALKVEEQRVLETVSGLTQVDARDLILKRTEDDVR
                     HDMAKMVRSVEEEARREGDRRARNILSLCI"
     gene            complement(901..1476)
                     /locus_tag="VFD74_04340"
     CDS             complement(901..1476)
                     /locus_tag="VFD74_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HZK48811.1"
                     /translation="MGLTEGTLLEGTSLKRVLDEGERGAARARALRYLETRERSRVEV
                     ERRLARYGYGPELVSEVVKWLAHLGYVDDRRFAQWLVRSRSQGSWGPRRITQDLLSRG
                     VERSIVKEAVGAARLAGADQAQVVDELAIVMQRRFAHDLGADPARAERRISAYLERRG
                     HGWDIIRLVVRRLSTSEMAAAEDDPGNAGVS"
     gene            complement(1613..2659)
                     /gene="recA"
                     /locus_tag="VFD74_04345"
     CDS             complement(1613..2659)
                     /gene="recA"
                     /locus_tag="VFD74_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZK48812.1"
                     /translation="MEKDKALEMAVGQIERQYGKGAIMRLGDEGAAVRIPAISTGSLG
                     LDLALGVGGVPRGRVIEIFGPESSGKTTLAFHIIAQAQKAGGKAAFIDAEHAMDPMYA
                     RRIGVQVDDLLLAQPDHGEQALEIAELLIRSGALDVLVVDSVAALTPRAEIEGEMGDS
                     HVGLQARLMSQALRKLAGTIHKTQTSAIFINQLREKIGVMFGNPEVTPGGRALKFYSS
                     VRLDIRRIETLKEGDKAVGNRVRVRVVKNKVAPPFRQAEFDVMYGTGVSVEGEILDMA
                     AENKVVQKSGAWYSYGDLRLGQGRENVKSFLREDPELVAKLRSEILDVIKPQFAYGDW
                     TAPTDEGEAPAPAE"
     gene            complement(2690..3250)
                     /gene="thpR"
                     /locus_tag="VFD74_04350"
     CDS             complement(2690..3250)
                     /gene="thpR"
                     /locus_tag="VFD74_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZK48813.1"
                     /translation="MTGERSRVFVGVPLPEEVLPFVEETQAALPDHADMRVTRAEQLH
                     LTLCFLGEVEATKVERAVRVVSAVPCTMGGVVLLGGYVCLPSEARARVVALDVDDPKG
                     VLTALFERVASGMEREGVFQREGRPFRPHLTIARLGRPGRLRPTSDVRRIPLGIRSVC
                     LYRSILSREGARYEALQSAALIDRSG"
     gene            complement(3240..3866)
                     /gene="pgsA"
                     /locus_tag="VFD74_04355"
     CDS             complement(3240..3866)
                     /gene="pgsA"
                     /locus_tag="VFD74_04355"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZK48814.1"
                     /translation="MRLNLPNSITIFRIFLIPVFMAFLLGAFPFGSDRARVDELSALF
                     SAGIFLVAAASDSLDGYFARRRRQVTVLGAFLDPLADKLLVSAALLALIELNRLSAWV
                     AMVIIAREFAVSGLRMVAAAEGVIIPASRWGKAKTASQMAAILAVILEPAYTIWGRTI
                     SWYLVALAVALTIWSGADYFLKARERLHLPEPSGSRLSADDSAVSDDG"
     gene            complement(3857..5200)
                     /gene="rimO"
                     /locus_tag="VFD74_04360"
     CDS             complement(3857..5200)
                     /gene="rimO"
                     /locus_tag="VFD74_04360"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZK48815.1"
                     /translation="MKALEGAYIHTLGCPKNEADSQTLAADLAASGVNIVESPETASH
                     ILVNTCGFIEDARAESIQAILEALQEFGPAQVLVMGCLVERYREELAAELPEVDGWYG
                     LGDVHALRDRLTAEAGSGALRAGSAASASAPQTASRSYAYLKISDGCDHLCSFCAIPQ
                     IKGPYHAIPPAEIVANARRSLTQGARELVLVGQDTAIWRDGDVDLPGLVDILAADQRV
                     ARVRLMYLQPEHVTADLLEFMATHDKLCRYLDIPLQHASTSVLRRMGRAGGAESYLAL
                     LDKARDLMPDVSVRSTFIVGFPGETDDDVETLIEFIGEAGLEHAGVFAYSAEEGTRAA
                     TLPGRVPARVVRDRLTLVAGAISDAAERAVAERVGKMVDVMMDRLGDDDGPEGAFAAG
                     RTCGQAPEVDGLTFLVGEGRRPSAVGEVVRVLLTESIGYDLVGTPIDAAEGSSCD"
     gene            complement(5214..>5740)
                     /locus_tag="VFD74_04365"
     CDS             complement(5214..>5740)
                     /locus_tag="VFD74_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HZK48816.1"
                     /translation="RGRRSQPNYVVVAVVALIVLAVLAIAFGNRGEKPAVIDPESVTS
                     STSTPAGGIGDAVGAGAATDESVAGVVVAMSAGAAEVPAADSNPDKLDLVIETVQNRC
                     WIVVREESAAGRTLYSGTLAEGERLTYSADGRIWLRIGDPSAVRLYVDGGLVEVPQPY
                     GDFVLSDAGLQRVE"
BASE COUNT         1003 a         2031 c         1708 g          998 t
ORIGIN      
        1 ggatacacag cgacaaaatg ttcctggccc gccggtctcc ctccctgcgc gcttcttcct
       61 ccacgcttcg caccatcttc gccatgtcgt ggcgtacgtc gtcttcggtg cgtttcagga
      121 tgagatcgcg agcgtcgacc tgggtgagtc cagagaccgt ttcgagcacc ctctgctctt
      181 cgaccttgag ggcttccacc tcctcgaggc gctcgcggta gtggatctca cgatcggcca
      241 ccgcctgttc gcggcgcccg acctccttga gtttctcgtc tagactctca tctcgctgga
      301 gcacccgctt ctccagctgc gaaagttcgt tccgacgatc gcgcagttcg gtctcggtgt
      361 ccgtgcgcaa cttgtgcagc tcgtccttcg ctccaacgag tgcttccctc ttctcggcct
      421 ccgcttggcg ctgggcggtc tccaggatct ttgaggcttc ttgctccgcg gaggtgatct
      481 ttgcctcccc gacgaacttg cgcagcagat agcccgcagc gatcccgatc gcccccaggg
      541 ccagggccaa cacgacccat agtccttcca tcggttctcc agtccattcc tattcggctg
      601 gtaacgctca cgtgtgatgc gacgaaccga tcgacaacat gaaaaagccg agcatagccc
      661 ggctgtacgg ctggttccgc gcccctctcg gattcctgag gctcagcgat tcacggtgtg
      721 accacggcgt tccgccggat cctccgcaac tgtgatggtc ccgatcttgt tgttgagtct
      781 tcgacctctg tcacacaacc gctgttgtcc aaaaaaatct atcgctacga gtggaacgtg
      841 ttcccgtcgt caaccgttcg tcacaagcag catcagttta tcggttctgt gaggaaagtg
      901 tcaagaaacc ccggcatttc ccggatcatc ctccgccgcg gccatttcgg aggtactcag
      961 gcggcgcacg acgagacgga tgatgtccca gccgtggcct ctccgctcca ggtaggcgga
     1021 tatgcgcctc tcggcgcgcg ccgggtccgc gcccaagtcg tgcgcgaaac gacgctgcat
     1081 gacgattgcc aactcgtcca cgacctgcgc ctggtcggcg ccggcaagcc gggccgcccc
     1141 cacagcctct ttgacgatgc tccgctcgac gccccggctc aggaggtctt gggtgatcct
     1201 gcgcgggccc caggatccct gcgagcgaga acgcaccagc cactgcgcga aacggcggtc
     1261 gtcgacgtag cccaggtgcg caagccactt caccacttcg ctcaccaact cgggtccgta
     1321 gccgtagcgg gccaggcggc gctccacttc tacccgcgac cgctcgcgcg tctccaggta
     1381 gcgcaaggcg cgggcgcgcg ccgcgccgcg ctcaccctcg tcgagtacgc gcttgagcga
     1441 ggttccctct aggagcgtcc cttcggtcag ccccatctcg gacaccagct ggaggtcgat
     1501 cgcaagacgc cgccccccct ccagctcgac gcgcgcccga gtgggatcct tcgggtcggg
     1561 ggccaggcgc tctatgcgag cgccggcgcc cgggtcgtca ctgtcactgt gctcattcgg
     1621 cgggtgcagg ggcctctccc tcgtccgtgg gtgcggtcca gtcgccgtat gcgaactgcg
     1681 gcttgatgac atctaggatc tcactgcgca gcttcgcaac caattcgggg tcttcgcgca
     1741 ggaagctctt cacgttctcc cggccttgcc ccaagcggag gtcgccgtag ctgtaccagg
     1801 cgccgctctt ctgcacgacc ttgttctcgg cggccatgtc gaggatctcg ccttctacgg
     1861 acacgccggt gccatacatg acgtcgaact cggcctgccg gaacggaggt gcgaccttgt
     1921 tcttcaccac ccggacccgg acccggttgc ccaccgcctt gtcgccctcc ttgagggtct
     1981 cgatgcggcg gatatcgagc ctcaccgagg agtaaaactt gagagcccgc ccaccgggag
     2041 tcacttccgg gttgccaaac atcacgccga tcttctcgcg aagctggttg atgaagatag
     2101 ccgaagtctg cgtcttgtgg atggtgccgg cgagctttct gagcgcctga ctcatgagcc
     2161 gggcctgaag ccccacgtga gagtcgccca tctcgccctc gatctcggct cgaggagtca
     2221 aggccgccac ggaatccacc accagcacgt cgagcgcccc gctgcggatg agcaactccg
     2281 cgatctcgag ggcctgctca ccgtgatcgg gctgggccag caggaggtcg tcgacctgga
     2341 ccccgatcct gcgggcatac atggggtcca tcgcgtgctc cgcgtctatg aacgcggcct
     2401 tcccccccgc cttctgcgcc tgagcgatga tgtggaaggc gagcgtggtc ttgccgctcg
     2461 attcgggacc gaagatctct atcacccggc cacgaggcac gccacccacc cccagagcca
     2521 agtcgagacc cagcgacccg gtggagatcg cgggaatgcg caccgcggcg ccctcgtcgc
     2581 cgagtcgcat gatcgcgccc ttgccatact gccgttcgat ctgccccaca gccatctcaa
     2641 gggccttatc cttctccacg tctcctccta gaggttgaac tccatccgat catccacttc
     2701 gatcgatcaa tgccgccgac tgcagagcct cgtatcgcgc tccttcacgg ctcaagatgc
     2761 tcctgtataa acacaccgag cgtatcccga gagggatgcg ccgcacatcg gacgttggtc
     2821 tcagtctgcc cggccgcccc agacgggcga tcgtgagatg cggcctgaac ggccgtcctt
     2881 ccctctggaa aacgccctcc cgctccatac cggaggccac ccgttcgaag agcgccgtca
     2941 agacgccctt cgggtcatcg acatccaggg ccacaactct cgcgcgcgcc tcggacggta
     3001 gacacacgta accgcccagc aacaccaccc cacccatggt acacggtacc gcggacacta
     3061 cccgaaccgc acgttccacc ttcgtcgctt cgacctcacc caggaagcac aaggtgaggt
     3121 gcagctgctc ggctcgggtg acacgcatat ccgcgtggtc cggtagcgcc gcctgcgtct
     3181 cttctacgaa ggggagcacc tcttcgggaa gagggacgcc cacgaagacc cgcgagcgct
     3241 cacccgtcat cgctgacggc ggagtcgtcc gccgagagcc gcgaacccga aggctccggc
     3301 agatgtagac gttcgcgggc cttcaaaaag tagtcagcac cactccagat ggtgagggcc
     3361 acagccaagg ccacaaggta ccaggagatc gtgcgccccc agatcgtata ggcaggctcg
     3421 aggatgaccg ccaggatagc cgccatctga ctggccgtct tggccttccc ccaccggctg
     3481 gccggtatga tcaccccttc ggcggcggcc accatgcgca ggcccgaaac cgcgaactcc
     3541 cgggcgatga tgaccatcgc cacccaggcc gagagacggt tgagttcgat caaggccagc
     3601 agcgccgcgg ataccagcag cttgtcggcc agcggatcga ggaaggcgcc caagaccgtg
     3661 acctggcggc ggcggcgagc gaagtatccg tcgagagagt cggaagcggc cgccaccagg
     3721 aagatgccgg cggagaagag cgcgctcaac tcgtccaccc gggctcgatc gcttccgaac
     3781 gggaaagccc ccaacagaaa cgccatgaag accgggatca ggaagatcct gaagatagtg
     3841 atcgagttgg gcagattcag tcgcatgagc tgccctccgc ggcgtcgata ggtgtgccca
     3901 ccagatcgta cccgatactc tccgtgagaa gaacgcgcac gacctcgccc actgcacttg
     3961 gccttcggcc ctcccccacc aagaaggtca ggccgtccac ctccggcgcc tgcccgcacg
     4021 tgcgcccagc ggcgaaggcg ccctcgggtc catcgtcgtc gccgagtcgg tccatcatca
     4081 cgtccaccat cttgcccacc cgctcagcta cggcgcgctc ggcggcgtcg gagatcgctc
     4141 cggctaccag cgtcagacga tcccgcacga ccctggcagg gacgcgcccc ggcagcgtcg
     4201 ccgctcgggt gccctcctcg gcactgtagg cgaacacccc ggcgtgctcg aggcctgcct
     4261 caccgatgaa ctcgatcaga gtctcgacgt cgtcgtccgt ctcccccgga aagcccacga
     4321 tgaaggtgct gcgcacggag acgtcaggca tgaggtcccg cgctttgtcc agaagggcga
     4381 gatacgactc cgccccgccg gctcggccca tgcgccgaag aacggacgtg gacgcgtgct
     4441 gcagggggat atccagatac cggcacagct tgtcgtgcgt cgccatgaac tcgaggaggt
     4501 cggcggtgac gtgctccggt tggaggtaca tcagacgcac ccgcgcgacg cgctggtctg
     4561 cggccagaat atccaccaag ccgggaaggt cgacgtcgcc atcgcgccag atggctgtgt
     4621 cttgacccac aaggaccagc tcgcgcgcac cctgggtgag gctgcgccgg gcgttcgcga
     4681 cgatctccgc gggtgggatg gcgtggtagg ggcccttgat ctgcgggatg gcgcagaagc
     4741 tgcacagatg atcgcagccg tcggagatct tgaggtaggc gtagctgcga gacgccgtct
     4801 gaggcgccga cgcggaggcc gccgacccgg cgcggagagc gccgctaccg gcctcagcgg
     4861 taaggcggtc gcgcagagcg tgcacatccc ccagaccata ccacccgtcc acctcgggaa
     4921 gctccgcagc gagctcctcc ctatagcgct cgaccaggca gcccatgacc agtacctggg
     4981 cggggccgaa ctcctgcaat gcctccagga tggcttggat cgactccgcc cgcgcatcct
     5041 cgatgaaacc gcatgtgttc acgaggatgt gtgaagccgt ctcgggactc tcgacgatgt
     5101 tgacaccgga agctgccaga tcggccgcga gcgtctgcga atccgcctcg ttcttggggc
     5161 aaccgagggt gtggatgtat gcgccttcga gggccttcaa cggcctctgc cactcactcg
     5221 acgcgctgga ggcccgcgtc gctcaggacg aaatcaccat acggttgcgg cacttccaca
     5281 agaccaccgt cgacatacaa tctcaccgcc gacggatccc cgatacgcaa ccagatgcgt
     5341 ccgtccgcgg agtaggtgag acgttctccc tccgccaacg tgcccgagta aagggtcctc
     5401 ccggcggcgg actcttccct caccacgatc caacaacggt tctggacggt ctcgatcacc
     5461 agatcgagtt tgtccgggtt cgagtcggcg gcagggactt ccgccgcgcc cgccgacatc
     5521 gcgaccacca cgccggctac gctctcgtcg gtcgccgccc ctgcgcccac cgcatcgccg
     5581 atgccacccg ccggagtcga cgtggaagag gtgacgctct ccgggtcgat caccgccggc
     5641 ttctctcccc ggttgccgaa ggcgatggcc agcaccgcga gaacgatcag agccacgacc
     5701 gcgactacca cgtagttggg ttgcgaccgc cgtcctctgg
//
LOCUS       DASRMJ010000156         9826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_485738, whole genome shotgun sequence.
ACCESSION   DASRMJ010000156 DASRMJ010000000
VERSION     DASRMJ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485738"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFD74_04370"
     CDS             complement(<1..849)
                     /locus_tag="VFD74_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48817.1"
                     /translation="MMLPSVPPGDSTYGWGDALVAARVVRTRSLGSLREVTLSVPGWG
                     GACAGQFAMLRLACSTRFLPRAFSVHTERPAEVGGDGARVTFLISCVGPGTAELATAP
                     VGSVVHVIGPLGRGFDLDALALAGAAAPPVLPASPVPPPPFPRLLIAGGGVGTAPLLL
                     VVEQLAERVGGGDACLGEVLVLLGFRDASQAAVLEVFEPAVAALAATGVRARLEAICE
                     DGALGREGLVTELLSYELRAGDVVVACGAHAMCRAVWELGVATGDVRAWFSLEAGMAC
                     GVGSCQG"
     gene            complement(860..4450)
                     /gene="carB"
                     /locus_tag="VFD74_04375"
     CDS             complement(860..4450)
                     /gene="carB"
                     /locus_tag="VFD74_04375"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZK48818.1"
                     /translation="MPRRDDIRKVMIIGSGPIVIGQACEFDYSGTQACKVLLEEGYEV
                     VLVNSNPATIMTDPAFSHRTYIEPLVPEVVARIIEKERPDALLPTLGGQTALNLAVAL
                     TEDGTLERFGVELLGADYAAIQRAEDRDQFRSTMASIGLRVPESAVVRDLRGAHQALE
                     DGLRLPLIIRPSFTMGGHGGGVAETRGDFDRAVQAGLDASPVGQVLLEESVIGWKEFE
                     LEVMRDRKDNVVIVCSIENMDPMGIHTGDSVTVAPQQTLTDVQYQRLRDASLDIIRAI
                     GVETGGSNVQFAYSPETDELMVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLD
                     EISNDITKKTPACFEPTIDYVVVKTPRWAFEKFPRADARLTTHMKSVGENMAIGRTFK
                     EAFQKSMRSRELDVKPDIPPSNDALVAMIAIPSAERYDLILEAYRRGVTLEQIFRATM
                     IDEWFLEELREIVEAEERLKKFASLPALAGHPPATPSPGHESQMGAAARACGPPLAPV
                     APAPPAAGEVAPESAATAGPPPPAGPQGRARASVATEAIAPAIDTLPREELRQAKRLG
                     FSDGRIAKLLGCTEDEVRARRLAEGIVPTYKAVDTCAAEFEAYTPYFYSTYEDENEAL
                     PNLERERVIILGSGPNRIGQGIEFDYCCVHAVMEMREQGYEAIMVNCNPETVSTDYDT
                     SDRLYFEPLTVEDVLNIVDNERPIGVIVQFGGQTPLRIAERLHRAGVPIMGTSQEAID
                     LAEDRERFGGVLRRLGLQAPPYGTARSAAEAIAIAEDIGYPVLVRPSYVLGGRAMEIV
                     YDRAALERYLEVSVKTPARHPILIDRFLERSMEVDVDAVCDGTDVYIGAVMQHVEEAG
                     VHSGDSACVIPPVTLGGEAVHAIEKQTEALALELGVIGLMNVQYALQSNDSGTTIYVL
                     EVNPRGSRTVPFVSKATGVPLAKIATQVIAGHKLAQLRPPGWDESLATGRVPSRRGLT
                     HVAVKEAVLPFQRFPGADSTLGPEMKSTGEVMGVGRSFPVAFGKASLAAGDSLPQGGT
                     VFLSVCDADKAAAVGVAATLARLGFRLLATAGTQGILARNGIDAELVLKYSEGLCDPA
                     GIGKAVTIVDLIERGEVDLVINTPRGGGARADGYEIRGAAVRRKVPATTTIAAAQAAV
                     QAIEAARTGEVPVACLQELHGPSLGSVMSGPFRRGPASTEPPTAHRPDRI"
     gene            4685..5533
                     /locus_tag="VFD74_04380"
     CDS             4685..5533
                     /locus_tag="VFD74_04380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZK48819.1"
                     /translation="MRRQLHLCAALLLAALVPGCAWPAGSTSTSAEPGGPAATTTIPW
                     ASTTVPPGELPRETLPPETPTTRTTQSPTTVTSLPAIPEGGVEYGRLPGDSKRVALTF
                     DASYDPTPLSSILATLESEGVPATFFFTGQFVEGFPEAVAAVVASGAPIGNHSYSHAD
                     FTKASPDEIRSQLQRTADLLTLAGADDPRPLFRFPYGARNKATLAILGSEGYVSVYWT
                     IDTLDWKPERTTQEIHDVVMAKLGPGAIVLMHVGGRETASVLPSLIRDIRAKGYEFVD
                     LRTALR"
     gene            5643..6806
                     /locus_tag="VFD74_04385"
     CDS             5643..6806
                     /locus_tag="VFD74_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK48820.1"
                     /translation="MLALRELLGSRLKFGLIAAAIGLVVALTLLMSAMSEGLITGMTG
                     AEGSLAGDALVFQRDTQLSLERSILTRDDLNILAVAPGVSNLYAVGHAMISVDAGAGV
                     FDARLLGLSAGFEQLPLVEGRPPQSPGEALVDITAKANGVALGDPVRLSPVDGTLTVV
                     GFTRDRRYVMSPVFYVDMATWRSLYVAALVGPAPGGSSAGGGAQGPGTAGVEERLSGE
                     ASIAAVRLDDGVTAADLAAALGDRYEVATPAAAARAGNGMPVMVLAVNGIQVVSLLIG
                     ALVIGIFFYITTLHKTGQIAAIKALGASNGYLYRQLLLQIVVLVTLAVVVGMALALGA
                     GASMPSTMAFDPGLGRWGVSLIAVYAMALLGSLFSLRNILSIDPAIALDRAEH"
     gene            6870..7502
                     /locus_tag="VFD74_04390"
     CDS             6870..7502
                     /locus_tag="VFD74_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48821.1"
                     /translation="MVTALSDVSLALDRGRLLAVLGPSGSGKTTLLSIIAGLLQPFAG
                     EILIEGQAIHLRSPAEAAAFRRRHVGLVFQDHHLVPYLTALENLLLVPHLAGRVREEH
                     RRRAERLLAEFGLLERLRHRPSALSGGERQRVAIARALMNEPTIMLVDEPTASLDTER
                     GQKVLELLRDQVHSRDMTCVMVTHDPRMAEQADQTVCLVDGRVAPGAGGE"
     gene            7600..8175
                     /locus_tag="VFD74_04395"
                     /pseudo
     CDS             7600..8175
                     /locus_tag="VFD74_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            complement(8291..9424)
                     /gene="oah"
                     /locus_tag="VFD74_04400"
     CDS             complement(8291..9424)
                     /gene="oah"
                     /locus_tag="VFD74_04400"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018807 -
                     6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HZK48822.1"
                     /translation="MSLDWIPRTNELKDHSLHTSEYWGTEAPCTMYVKSPLTGPDGEV
                     VPGLYVARITLNNPKQFNSYTTEMVKGVIAGFTNASLDRSVVAVVFTAVGDKAFCTGG
                     NTVEYAEYYSKRPQEYGEYMDLFNAMVDSILNCKKPVIARVNGMRVGGGQEIGMACDL
                     AVSSDMAIYGQAGPKHGSAPVGGSTDFLPWFLSMEDAMWNCISCELWSAYKMKAKNLI
                     SKVVPVLRTAEGDLIRNPLVHTQSYVEDGEIVYGEMKTGEAAAAAKEIVKSCTVDFTE
                     LDKSVDAIVWKFANLFPESLQMSIDSVRMKKRFFWDMAKTPSRHWLATNMMVEAYLGF
                     NAFSTRKLTGADTVDFLKFRQLLADGALMDDDAFEQVMGTPKE"
     gene            complement(9462..>9826)
                     /locus_tag="VFD74_04405"
     CDS             complement(9462..>9826)
                     /locus_tag="VFD74_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZK48823.1"
                     /translation="SVVDYTQTAVDGRFMGEGPGVAIPKALKRAGMSLSNMDLIEVNE
                     AFAAQVLANERVLGWDRDKLNIHGGAIALGHPTGCSGARILVTLYHALKRTGGELGVA
                     AICGGGGATMATIIRRES"
BASE COUNT         1631 a         3416 c         3155 g         1624 t
ORIGIN      
        1 cccttggcac gatccgaccc cgcacgccat ccccgcctcc aagctgaacc aggcccgcac
       61 gtcgcccgtg gccacaccta gctcccagac cgcccgacac atcgcgtgcg ccccgcacgc
      121 caccaccacg tcgccggcgc gaagctcgta agacagcagc tccgtgacaa ggccctcccg
      181 gccgagggca ccgtcctcgc agatggcctc gagccgcgct cggaccccgg tcgcggccag
      241 ggcggccact gcgggctcga acacctcgag cacagcggcc tgagaggcat cgcggaatcc
      301 taggagcacc aggacttccc cgaggcacgc gtctcctccc cccacacgct ccgcaagctg
      361 ctccaccacc aacaagagcg gcgcggtgcc gacgccgccg ccggcgatga gaaggcgtgg
      421 gaagggtgga ggcggcacgg gtgacgcggg taacacgggt ggcgcggcag ctcccgccaa
      481 ggccaacgca tccaggtcga aaccgcgccc caggggaccg atgacgtgca ccacgctccc
      541 caccggagcg gtggcgagtt ccgcggtgcc gggaccgacg caagagatca ggaaggtgac
      601 acgagctccg tccccgccca cctcagcggg cctctcggtg tgcacgctga acgcgcgagg
      661 caggaaccgg gtggagcagg cgagccgcag catcgcgaac tgccccgcac atgcgcctcc
      721 ccatccgggc actgagagag tcacctccct caaggatccg agactgcggg tgcggaccac
      781 ccgggccgcg acgagggcat ccccccagcc gtaggtggag tccccgggag gaaccgaggg
      841 cagcatcacg agcccatcct caaatccggt cgggccggtg ggcggtcggc ggctcagtgg
      901 aggcggggcc gcgacggaac ggaccgctca tcaccgaccc cagtgacggc ccgtgcaact
      961 cctgcaaaca tgccaccggc acctcgccgg tgcgcgccgc ttcgatcgcc tgcaccgccg
     1021 cctgcgcagc cgctatcgtc gtggtggcgg gcaccttgcg tctgacggcg gctccgcgga
     1081 tctcgtaccc gtcggcacgc gccccaccac cacggggggt gttgatcacc aggtcgacct
     1141 ctcctcgctc gataagatcg acgatcgtga ccgccttgcc gattcccgct gggtcgcata
     1201 gaccctccga gtacttaagc accaactccg cgtcgatgcc gttgcgagcg aggatgccct
     1261 gggtgccggc cgtagcaagc agccgaaagc ccagccgggc cagggtggcc gccaccccca
     1321 ctgcagccgc cttgtccgca tcgcacaccg acaggaatac cgtgcccccc tgcggcagac
     1381 tgtcgccggc tgccagggag gctttcccga aggctacggg gaagctgcgc ccgacgccca
     1441 tcacctcgcc ggtcgacttc atctccgggc ccagtgtgct gtccgcgccc gggaagcgct
     1501 ggaacgggag caccgcctcc ttgacggcca cgtgcgtcag gccacgccga ctcggcacgc
     1561 gccccgtagc caaggactcg tcccagccgg gcggtcgaag ctgggccaac ttgtggccgg
     1621 cgatcacctg agtggcgatc ttggccaagg gcacgcccgt ggccttgctc acgaacggca
     1681 cggtgcgact cccgcgaggg ttcacctcga gcacgtagat ggtggtgccg gagtcgttcg
     1741 attgcagcgc gtactggacg ttcatgaggc cgatgacccc gagttccagc gccaaggctt
     1801 ccgtctgctt ctcgatggcg tggaccgctt cccccccgag cgtcaccggt gggatcacac
     1861 acgcggagtc tccggagtgc acacccgcct cttccacgtg ttgcatcacg gcgccgatgt
     1921 agacgtctgt gccgtcgcac acggcatcca cgtccacctc catgctgcgc tccagaaagc
     1981 ggtcgatgag gatcgggtgc cgcgcaggcg tcttcaccga cacctcgagg tagcgttcaa
     2041 gcgccgccct gtcgtatacg atctccatcg cccgaccgcc gagcacgtag ctggggcgca
     2101 cgaggaccgg gtagcctatg tcctccgcga tggcgatagc ctcagccgcc gaacgggcag
     2161 tgccatacgg gggtgcctgc aggcccagcc tgcgcaggac gccgccgaaa cgttcgcggt
     2221 cctccgccag gtcgatcgcc tcctggctcg tgcccatgat gggcactccc gcccggtgca
     2281 gacgctcggc tatgcgcaag ggagtctgcc ctccgaactg gacgatgacg cctatgggcc
     2341 tctcgttgtc gacgatgttg agcacgtcct ccacggtgag cggctcgaaa tacaggcggt
     2401 cggaggtgtc gtagtcggtg gagaccgtct caggattgca gttcaccatg atcgcttcgt
     2461 agccctgctc acgcatctcc atcaccgcgt gaacgcagca gtaatcgaac tcgatgccct
     2521 ggccgatacg gttcgggccg ctcccaagga tgatcacccg ctcgcgctcg aggttgggga
     2581 gtgcctcgtt ctcgtcctcg taggtggagt agaagtaggg cgtgtaggct tcgaactcgg
     2641 cggcgcaggt gtccaccgcc ttgtacgttg gcacgatgcc ctccgcgagg cggcgtgcgc
     2701 gtacttcgtc ctcggtgcag ccgaggagtt tggcgatgcg gccgtcgctg aatccaagac
     2761 gcttggcctg gcgcagctct tccctcggca aagtatcgat ggccggagcg atggcctccg
     2821 tagcgacgga ggcgcgagcg cggccctggg gtccggccgg cggtgggggg cccgcggtcg
     2881 ccgcgctctc gggcgcgacc tctcccgccg ccggcggcgc gggcgcgacg ggcgcgagcg
     2941 gcggcccgca cgccctcgca gccgccccca tctgtgactc gtgccccgga cttggggtcg
     3001 ccggcgggtg gccggcgagg gcagggagcg acgcgaactt cttcaaccgc tcctccgcct
     3061 ccacgatctc gcgcaactcc tcgagaaacc actcgtcgat catagtggca cggaagatct
     3121 gctccagcgt gaccccccgc ctatatgcct ccaggatgag gtcgtagcgc tccgccgatg
     3181 ggatggcgat catggccacc aaggcgtcgt tgctcggggg gatgtcgggt ttcacgtcga
     3241 gttcccgaga acgcatcgac ttctggaacg cctccttgaa cgtgcggccg atggccatat
     3301 tctcccccac gctcttcatg tgggtggtga ggcgggcgtc ggcccggggg aacttctcga
     3361 aggcccagcg cggcgtcttc accaccacgt agtcgatggt gggctcgaag cacgccggtg
     3421 tcttcttggt gatgtcgttg gagatctcgt cgagcgtgta gcccaccgcc agcttcgcgg
     3481 cgatcttggc tatggggaag cccgtggcct tcgaagcgag agccgagctc cgcgagacgc
     3541 gcgggttcat ctcgatgacc atcagttcgt cggtctcggg agaataggcg aactggacgt
     3601 tcgaaccgcc ggtctccact ccgatggccc ggatgatgtc gagagaagcg tcgcgcaggc
     3661 gctggtactg gacatcggtg agggtctgtt gaggagccac ggtgaccgag tcgccggtgt
     3721 ggatgcccat ggggtccatg ttctcgatcg agcagacgat gaccacgttg tccttgcggt
     3781 cgcgcatgac ctcgagctcg aactccttcc agccgatgac cgactcctcg agcagcacct
     3841 gacctaccgg cgacgcgtcg agccctgcct gcaccgccct gtcgaaatcg cccctggtct
     3901 cggcgacccc gccgccgtgg ccacccatcg tgaagctcgg gcggatgatc aagggcagac
     3961 gcagcccgtc ctcgagcgct tggtgcgcgc cccgaaggtc ccgcaccacc gccgactcgg
     4021 gaacccggag gccgatggat gccatggtgg agcggaactg gtcccgatcc tcggcacgct
     4081 ggatagcggc atagtcggca ccgaggagct ccaccccgaa gcgctccagg gtgccatcct
     4141 ccgtcagggc gacagccagg ttaagggcgg tctggcctcc aagtgtgggc aagagggcgt
     4201 cggggcgctc cttctcgatg atgcgggcga ccacctcggg cacgagcggc tcgatgtagg
     4261 tgcggtgaga gaaggctggg tccgtcatga tggtggccgg gttggagttc acgaggacaa
     4321 cttcgtagcc ctcctccaac agcaccttgc acgcctgggt ccccgagtag tcgaactcgc
     4381 acgcctgccc gatgacgatg gggcccgacc cgatgatcat gaccttgcgg atatcgtctc
     4441 ggcgtggcat cagaatgttc ccccaggtca ctcggcggat gtacagttcg ggatggggcc
     4501 cgccaggttc acgcaagggt cgcggacggg tagggcgatg ataccaggac ggcgcccgcg
     4561 ggagagcgtc gggcggactc tcggaggcgg cgcgccggac atcaatcggc ccggcggcac
     4621 ctgcaccacc gggactgcgg cagtcgaagc gaaaggggag ggcatgaacc atcggtcaag
     4681 gtgcatgagg cgacagctcc acctctgcgc cgcccttctg ctggcggcct tggtgccagg
     4741 ctgcgcctgg ccggcagggt cgacctccac gagcgcggaa cccggcggcc ccgcggcgac
     4801 caccaccatc ccgtgggcca gcaccaccgt gcctccgggc gaactccctc gtgagactct
     4861 ccctcctgag acgcccacca cccgcaccac tcaatcgccc accaccgtca ccagcctgcc
     4921 ggcaatcccc gaaggtggtg tcgagtacgg tcgcctgccc ggggactcca agcgagtagc
     4981 tctcaccttc gacgcctcct atgatccgac cccgctctcg tccatcctgg ctacgctcga
     5041 gagcgaggga gtccccgcca ccttcttctt caccgggcag ttcgtcgagg gtttcccgga
     5101 ggccgtggcc gctgtcgtgg cctccggcgc ccccatcggg aatcactcct actcgcatgc
     5161 agacttcacc aaggcctccc ctgacgagat ccgttcacaa ctgcaacgca ccgccgacct
     5221 gctcaccctc gccggtgctg acgatccgcg gccgctgttc cgcttcccct acggtgcacg
     5281 aaacaaagcg acgctggcca tcctcggcag cgagggctac gtcagcgtct actggacgat
     5341 cgacaccctt gactggaagc cggaacgcac gactcaggag atccacgacg tcgtcatggc
     5401 caagctcggt ccgggcgcga tcgtgctgat gcacgtgggc ggtcgggaga cggcttcggt
     5461 cttgccctcg ctcatccgag acatccgcgc caaaggatac gagttcgtgg acctgcggac
     5521 ggccctccga tgatcagccg accatacgct cacccagcct ggtggccgaa ccttccagct
     5581 cccgtgcgct atcatcgacc ggtcccgctg cgctcgaccc accaagcacc gagaggacca
     5641 tcgtgctggc actcagagaa ctactcggca gcagactcaa gttcggcctg atcgcggccg
     5701 cgatcggcct ggtcgtggct ctcaccctac tcatgtcggc catgtcggag ggcctgatca
     5761 ccgggatgac cggagccgag ggctctctcg ctggcgacgc actcgtgttc caacgcgaca
     5821 cccagctgtc cctcgaacgc agcatcctca cgcgtgacga cctgaacatt ctcgcggtcg
     5881 cgcccggcgt gtccaacctg tacgcggtcg gccatgccat gatctcggtg gacgcgggtg
     5941 ccggagtgtt cgatgcccgc ctcctcggac tgagcgcagg cttcgagcaa ctaccactcg
     6001 tggaagggag acccccacag tccccggggg aagccctcgt ggacatcacg gccaaagcca
     6061 acggcgtcgc actgggcgat cccgtgcgcc tctcccccgt cgatggcacc ctcaccgtcg
     6121 tgggcttcac ccgcgatcgc cgctacgtca tgtccccggt cttctacgtc gacatggcca
     6181 cctggcgaag cctctacgtg gcggccctcg tggggcctgc gcccggaggc tcaagtgcag
     6241 gaggcggcgc gcaaggcccg ggcacagcgg gggtggaaga acgactctcc ggcgaggcct
     6301 ccatcgccgc cgtccgcctg gatgacggag tcacggctgc cgacctcgcg gcggcgctgg
     6361 gagaccggta cgaggtggcc acacccgccg ccgccgcccg cgccggcaac ggcatgccgg
     6421 tcatggtcct ggcggtgaac ggcatacagg tggtgtccct cctgataggc gctctggtca
     6481 tcgggatctt cttctacatc accaccctcc acaagaccgg ccagatcgca gcgatcaaag
     6541 cgctgggggc ttcgaacggc tacctctacc gccaactgct gttgcagatc gtggtcttgg
     6601 tgacgttggc cgtggtcgtg ggcatggcgc tggcgttggg agccggcgcg tcgatgccgt
     6661 cgacgatggc gttcgacccc gggctgggac gatggggcgt atcgctcata gccgtgtacg
     6721 cgatggcact cctcggaagc ctcttctcac tccggaacat cctcagcatc gaccctgcca
     6781 tcgccctcga ccgcgcggaa cactaggaga acgacatcaa tctgctagag gtcaagaagg
     6841 tctcgaaaca ctacggcagc ggccacacca tggtgaccgc gctcagcgac gtctcgctcg
     6901 ccctggatcg gggacggctc ctcgcggtgc tcgggccttc cggctctggg aagaccaccc
     6961 tactctccat catcgccggg ctgctgcaac cttttgcggg ggagatcctc atcgaaggcc
     7021 aagcgatcca ccttcgcagc ccggcggaag cggcggcctt ccgaaggcgc catgtgggcc
     7081 ttgtgttcca ggaccatcac ctggtcccgt atctgaccgc cctcgagaac ctcctcctcg
     7141 ttccccatct ggccggaagg gtgcgggagg aacatcggcg gcgcgcagag agactcctcg
     7201 ccgaattcgg actcctcgag cgcctcaggc acagaccctc agcactgtcc ggtggcgagc
     7261 ggcagcgagt cgcgatcgcc cgggccctca tgaacgaacc gacgatcatg ctggtggacg
     7321 agcccacggc gagcctggac accgaacgcg gtcagaaggt actcgaacta cttcgcgatc
     7381 aggtccactc gcgggacatg acgtgcgtca tggtgactca cgaccctcgc atggccgaac
     7441 aagccgacca gaccgtctgc ctggtcgatg ggcgggtcgc tccaggcgcg ggcggcgagt
     7501 gacgggcaag cggccgcaac acctccgaac cagaaggagc caacaatcat gacgacccgc
     7561 atcaccatct tctcggacta catctgaccc ttctgctatg tcggcgcggg catcgtcgac
     7621 caactgaagc aggagttcga catcgaggag gagtggatca gctacgagat ccacccggag
     7681 acgccggccg aaggccggtc cttggccgag tacttcgggg cggccgacct gcgcccgatg
     7741 aacgacatgc tgcgggcgcg gacggccgag ttcggcctgt cgttcgcgcc gccgctcgtc
     7801 ctatccaact cgcgttcagc catcttggcc gcggaatacg cacgcagcac gggcagccac
     7861 gcggagttca gtcgccgcgt cttcgccgcc tacttcgctg aaggacgaga catcggcgat
     7921 gtggccactc tccgggccgt agccgccgac gcgggcctgg atgcggaggg tttgcgactc
     7981 tccctgctcg ccggagagta cgtggacctc ctgttggcgg ccgaggccga agcccgcggc
     8041 ctcggcatca ccggcgtgcc cacgttcttc gttgggggag tgccctcatc ttcggatgcg
     8101 tcgcgggctc cgagtcgaag gatcgtgggc gcgcagccgc tggaggtgtt ccggacggct
     8161 ctcaggggga tgtagacctt cccagccgct ctctcagccg ctgctatctc tccgccgcta
     8221 ccccgcccta cagcgacgcc ccgaagtcgc gggactccag ggcgtcgaag atgcgcaatc
     8281 tgactcggag ctactccttc ggcgtgccca tcacctgctc gaacgcgtcg tcgtccatga
     8341 gggcgccgtc ggccagcagt tgccggaact tcaggaagtc gacggtgtcg gcgccggtga
     8401 gcttgcgggt ggagaacgcg ttgaaaccga ggtaagcctc caccatcatg ttcgtcgcaa
     8461 gccagtgccg actcggcgtc ttcgccatat cccagaagaa ccgcttcttc atgcgcacgg
     8521 agtcgatgga catctgcagc gactcgggga agaggttggc gaacttccac acgatggcgt
     8581 cgacagactt gtcgagttcc gtgaagtcga cggtgcaact cttgactatc tccttggccg
     8641 cggcggcggc ctcgcccgtc ttcatctcgc cgtagacgat ctccccgtct tcgacgtagc
     8701 tctgagtatg aacgagcggg ttgcggatca ggtcaccctc ggccgtgcga agcacgggca
     8761 ccaccttcga aatgaggttc ttggccttca tcttgtacgc ggaccacagc tcgcacgaga
     8821 tgcagttcca catggcgtct tccatggaga ggaaccaggg caggaagtcg gtggatccgc
     8881 ccaccggcgc cgagccgtgc ttcgggccgg cctggccgta gatggccatg tcggaggaga
     8941 ccgccagatc gcacgccatg ccgatctcct ggccgccgcc cacgcgcatc ccgttgaccc
     9001 gcgcgatcac cggcttcttg cagttcaaga tcgagtcgac catggcattg aacaggtcca
     9061 tgtactcgcc gtactcctgg gggcgcttgg agtagtactc cgcgtactcc accgtgttgc
     9121 caccggtgca gaaggccttg tcgccgaccg cggtgaagac gacggctacg acgctgcggt
     9181 cgagagaggc gttggtgaag ccggctatca cgcccttgac catctccgtg gtgtagctgt
     9241 tgaactgctt ggggttgttc agggtgatgc gcgccacata caggccggga acgacctcgc
     9301 cgtcagggcc ggtcaggggc gacttgacgt acatcgtgca cggggcttcc gtgccccagt
     9361 actccgacgt gtggaggctg tggtccttga gttcgttggt cctcgggatc caatccaggc
     9421 tcatctctgc tcctctcgat ctcgccgcgg cgcgggcggg gtcaggattc tctccgtatg
     9481 atggtggcca tggtcgcccc gccgcctccg cagatggcgg cgacaccaag ctcaccaccg
     9541 gtgcgcttga gggcgtgata cagcgtcacg aggatgcggg cgccggaaca gccggtggga
     9601 tgcccgaggg cgatcgcgcc gccgtggatg ttgagcttgt cacggtccca gccgaggacg
     9661 cgctcgttcg ccagcacctg cgcagcaaag gcctcgttca cctcgatgag gtccatgttg
     9721 gagagcgaca tccccgcccg cttcagggcc ttagggatgg ccaccccggg gccctcgccc
     9781 atgaagcggc catcgaccgc cgtctgcgtg tagtcgacca ctgaaa
//
LOCUS       DASRMJ010000157         6834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_485827, whole genome shotgun sequence.
ACCESSION   DASRMJ010000157 DASRMJ010000000
VERSION     DASRMJ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485827"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFD74_04410"
     CDS             <1..281
                     /locus_tag="VFD74_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48824.1"
                     /translation="ISNLVEDRDRASWRIEEAAEVAARATLMVCARIDEVLVAFRAAG
                     DEDDAAGDDHDAAREEDDAAGDGSHAGDAGSDTSGFDAADSSAGGCFS"
     gene            278..1234
                     /locus_tag="VFD74_04415"
     CDS             278..1234
                     /locus_tag="VFD74_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HZK48825.1"
                     /translation="MSALSLAYSPCPNDTYIFHAWVHGLVPGAPRVREVLADIDELNA
                     MALRAEPDVVKVSFHALAHLRERYVLLHSGGALGRGCGPLVLVTPESPLRLANGGGPS
                     RVLAGRRVAIPGELTTAALLLRLYAPEVGEMVPMPFHRIMGSVRRGEVDAGVIIHEGR
                     FTYVQQGLRSLLDLGDWWEGVTGRPIPLGGIVVRRDLGPDVARRVDDAVRRSLEVARA
                     DPEASTAYIRRHAQEMDPKVCREHIALYVTDFSIDYGDEGEGAIRHLLAQAEVLGAVP
                     DSGDRPLFWDDPAAMGFRADGPVGPAGADGPDGADGPGGADR"
     gene            complement(1231..2256)
                     /gene="add"
                     /locus_tag="VFD74_04420"
     CDS             complement(1231..2256)
                     /gene="add"
                     /locus_tag="VFD74_04420"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZK48826.1"
                     /translation="MIDPSIPLIDLHRHLDGNVRLETILDLASQHGVALPAQDVEGLR
                     EAVQVTRPLDDLLQFLAKFSWLTAVMVDEAACRRIAYENVEDALREGIDYVELRFSPW
                     FMAETHGLDPQAVVEAVVDGATAGARDTGVMVKLIGILSRTYGPANATRELEALLAHR
                     DALVALDLAGDEVRVPGALFVDHFRRARDAGLEVTVHAGEAAGAESVWQALDSLGARR
                     IGHGVRAIDDPALVERLAAERIGIESCLTSNVQTGTVPSFAEHPLRRFLAAGVPATIN
                     TDDPAISGIDLANEYEVAAPAAGLSPKWTRQAQLNALEVAFLTDDERRQLLQKKRGAA
                     EPGPAEG"
     gene            2381..4348
                     /locus_tag="VFD74_04425"
     CDS             2381..4348
                     /locus_tag="VFD74_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZK48827.1"
                     /translation="MMHINDISKSFGTQVILDQASLHIRPGMRIGLVGPNGAGKTTLF
                     RIIAGEMSLDEGDINARKGLRIGFLPQEIEEIAEFAVLDEVLASYADILDMEHRLKGL
                     GERISAAYEGDSSEDADALMNELGSLQTAFEHAEGYELESRAQSILRGMGFNDDDFDR
                     PIKELSGGWRMRVALARLLMETPDLLLLDEPTNHLDLESLLWLEEFLLEWTGSLVLVS
                     HDRYFLNRMVTHIVEMDRGGLDLYTGNYDHFEIEKQQRYEALSNAAKNQQREIESAES
                     FVRRFRAKNTKAKQVQQKVRQLEKMERIDAPNLERKKIRFRFPQPGRTGRVVVEMQRV
                     RKAYGDTVVYKGLDLVVERGEKIALVGPNGAGKSTLLKLLAGAITPDGGSLKLGHNVR
                     REYFAQHQLEVFDPDRSVLKTMEDVAAPVDKMTMVRGYLGTFLFTGDDVEKKVGVLSG
                     GEKARLALARMLLDPAGLLLLDEPTNHLDMASREVLSEALRQFEGSVIFISHDRHLIN
                     AIATEVIEVKAGRLTHYIGDWEYYQWKSGRAGAVPPTARLGTRSEAAGIEDAGSSAVG
                     LPAADTPGVGSPSYRERKDMQRRHRQLEKRIMALEDRQRELGGLLADPAHAADYELLM
                     SASAEATEIKDELAGLYPEWEVVTERVAALE"
     gene            complement(4452..5531)
                     /locus_tag="VFD74_04430"
     CDS             complement(4452..5531)
                     /locus_tag="VFD74_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZK48828.1"
                     /translation="MAVAPDPALAATTLAQTESELAAAKTELRARQSELDKLAQRYAT
                     AQDELATTEDRVTEVQAQADRAAADLEIMRARLNDRLRGMYMDGDAGGLAILGVFFDG
                     DSLAGILNRLAMLGRVLEGDDDVLSQVELQVAKLDSLEAELSDKRLLQTEQLAEIDKA
                     NEAAMKALEDSKSEYNTLREKVARLQEEARKKREEAARLAAAAAAQSSGSSGSSGSTT
                     GRAVSGSGWAFPVQGPNSFINDWGFARSGGRSHRGTDIMTARNTHVVAVVAGVIKRTS
                     PTERSLGGITIWLDGGDGNSYYYAHLTSIASSITPGTRVSAGQVIGYAGNTGNARGGE
                     VHLHFEIHPGGGSAINPYPTLVANR"
     gene            5971..6717
                     /locus_tag="VFD74_04435"
     CDS             5971..6717
                     /locus_tag="VFD74_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48829.1"
                     /translation="MPLLSAIDWSVARGEHWAVLGPNGSGKTTLLRLVAGYVQPTQGA
                     AEVLGGVFGRTDLRVLRRRIGWVSPAMADRLHPGDPALDVVLGGIFATVGLFHESPTH
                     DDRAKAGDLLEALGCGSLGPRRFGVLSQGERQRVLLARALISDPSLLVLDEPTAGLDV
                     AAREDLLESLTVLASASDGPTILLVTHHLEEIVPGFSHALLLSGGRVLAAGPRQRVLT
                     APLVSSALGVDVEVVERDGRLWSIVRRNGG"
BASE COUNT         1053 a         2191 c         2420 g         1170 t
ORIGIN      
        1 ggataagcaa cctcgtggag gatcgggatc gggcctcgtg gaggatcgag gaggcagcgg
       61 aagtcgcagc gcgtgccacg ctcatggtgt gtgctcggat cgacgaggtc ttggtggcct
      121 ttcgggccgc cggcgacgag gacgacgccg ctggagacga tcacgacgca gccagagaag
      181 aggacgacgc ggccggagac ggctcccatg cgggtgacgc cggctccgac accagtggct
      241 tcgatgcggc cgattccagc gcgggcggtt gtttcagttg agcgcgttga gcctggccta
      301 ctcgccgtgt cccaacgaca cgtacatctt ccacgcctgg gtgcacggcc tggtgccggg
      361 agcgccgcgt gtgcgggaag tgctcgcgga catcgacgaa ctgaacgcga tggctctgcg
      421 ggctgaaccc gatgtcgtca aggtgtcgtt ccacgctctg gctcaccttc gcgagcgcta
      481 cgtcctcctg cactccggtg gggcgctggg ccgtgggtgt ggcccgctcg tcctcgtgac
      541 acccgaatcg ccgctgcgtc tcgcgaacgg gggcgggcct tctcgggtcc tggccggccg
      601 gagggtggcg atacccggag agttgaccac agcagccctg ctcctgcgtc tctacgctcc
      661 ggaggtcggc gagatggtgc cgatgccgtt ccaccggatc atgggttcgg tgcgaagggg
      721 ggaggtcgac gcgggggtga tcatccacga aggccgattc acgtacgtgc agcaaggact
      781 ccggagcctg ctcgatctgg gcgactggtg ggagggcgtg acgggccgcc cgataccgct
      841 gggtgggatc gtcgtgcgcc gggacctcgg gcctgacgtg gcccgccgcg tggacgacgc
      901 ggtgcggcgg agcttggagg tcgcgcgcgc cgacccggag gcgtccaccg cgtacatccg
      961 gcgccacgct caggagatgg acccgaaggt ctgtcgcgag cacatagcgc tgtacgtcac
     1021 cgacttctcg atcgactacg gtgatgaggg ggaaggggcc atccgccacc ttctggcaca
     1081 ggccgaagtc ttgggtgcgg tgcccgattc cggcgaccga ccgctgttct gggacgatcc
     1141 tgcggcgatg ggatttcgcg cggacggccc ggtgggccca gctggcgctg atggcccgga
     1201 cggcgccgat ggcccgggtg gcgccgatcg ctagccttcc gcggggcccg gctcggcggc
     1261 cccgcgcttc ttctggagga gctgccgcct ttcgtcgtcg gtgaggaagg cgacctccag
     1321 cgcgttcagc tgtgcctgcc tggtccactt cggggagagg ccggcggcgg gcgcggccac
     1381 ttcgtactcg ttggccaggt cgatgccact gatggcgggg tcgtcggtgt tgatggtggc
     1441 gggcacacct gcagcgagga agcggcgcag cgggtgttcg gcgaacgagg ggactgtgcc
     1501 cgtctgcacg ttgctcgtga ggcacgattc gatgccgatc cgttcagcgg ccaatcgctc
     1561 caccagggcc gggtcgtcga tggcccgaac cccgtgcccg atgcgccggg caccgaggga
     1621 gtccaaggcc tgccacacgc tctccgctcc ggctgcctcg cccgcgtgca ccgtgacctc
     1681 caggcccgcg tcccgagctc ggcggaagtg gtccacgaag agcgccccgg ggacacggac
     1741 ctcgtctccc gcaagatcga gcgccacgag cgcgtcacgg tgggcgagga gtgcctccaa
     1801 ctcccgggtg gcgttggcgg ggccgtacgt ccggctgagg atccctatga gcttgaccat
     1861 cacgcccgtg tcgcgcgcgc ccgcggtcgc tccgtctacc acggcttcga ccacggcctg
     1921 cggatcgagg ccatgggtct cagccatgaa ccacgggctg aagcggagtt ccacgtagtc
     1981 gatgccttcg cgaagcgcat cctcgacgtt ctcgtaggcg atccgcctac aggcggcttc
     2041 gtccaccatc acggcggtga gccaggagaa cttcgcgagg aactgcagga ggtcatcgag
     2101 cgggcgggtc acctgcaccg cctcacgtag accctcgacg tcctgcgcgg gtagggcgac
     2161 gccgtgttga gacgccaggt cgaggatggt ctcgaggcgc acgttcccgt cgaggtgccg
     2221 atgaaggtcg attaggggga tagaagggtc gatcacgcgg tggtctcctg acgccgaggg
     2281 acgatggctc gctcagcgcc gcctatcccc actccggtcc ccgttgaatc gacggcccgc
     2341 gcgggtcggg agagcggcat cctgctatcc ttcctcggtc atgatgcaca tcaacgacat
     2401 ctccaagtcc ttcggcaccc aggtcatcct cgaccaggcg agcctccaca tccgccctgg
     2461 gatgcgcatc ggcctggtcg gcccgaacgg cgccggcaag accaccctct tccgcatcat
     2521 cgcgggcgag atgtcgctgg acgaagggga catcaacgcg cgcaaaggcc tgcgtattgg
     2581 cttcctccct caagagatcg aggagatcgc cgagttcgcg gtgctcgacg aggtgctcgc
     2641 ctcctacgcc gacatcctgg acatggaaca ccggctgaag ggattgggag agcgtatctc
     2701 cgccgcctac gagggcgact ccagtgagga cgccgacgct ctcatgaacg agttgggctc
     2761 cttgcagacc gcgtttgaac acgccgaagg atacgaactc gaatctcgcg cgcagtcgat
     2821 cctgcgagga atgggattca acgacgacga cttcgatcgg cccatcaagg agctgtccgg
     2881 tggctggcgc atgcgggtgg ccttggcccg tctgctgatg gagacgcccg acctcctgct
     2941 cttggacgag cccaccaacc atctggatct cgagagtctg ctctggcttg aggagttcct
     3001 gctcgagtgg actggatcgt tggtcctcgt gagccacgac aggtatttcc tgaaccgcat
     3061 ggtcacccat atcgtcgaga tggatcgggg cggcctcgat ctctatacgg gcaactacga
     3121 tcatttcgag atcgagaagc aacagcgcta cgaggcactc tcgaatgccg ccaagaacca
     3181 gcagcgggag atcgagtcgg cggagagctt cgtgcgccgg ttccgcgcca agaacacgaa
     3241 ggccaagcag gtgcaacaga aggtgcgcca gctcgagaag atggagcgta tcgacgcgcc
     3301 caatctcgag cgcaagaaga tcaggttccg cttcccgcag ccgggacgca cggggcgggt
     3361 cgtggtcgag atgcagcggg tgcggaaggc ctacggagac accgtggtgt acaagggcct
     3421 cgacctcgtc gtggaacggg gcgagaagat cgctctggtg ggccccaacg gagcgggcaa
     3481 gtcgacgctg ctgaaactgc tggctggagc catcacgccg gacgggggct ccctgaagtt
     3541 gggtcacaac gtgcgccgcg agtatttcgc gcagcaccag ctggaggtgt tcgaccccga
     3601 ccgcagcgtt ctcaagacga tggaggacgt ggcggctccc gtcgacaaga tgaccatggt
     3661 cagaggctat ctgggcacct tcctcttcac cggcgacgac gtcgagaaga aagtgggggt
     3721 gctctcggga ggagagaagg cgcgtcttgc gttggcccgc atgctgctcg atccggcggg
     3781 acttctcctg ctcgacgagc ccaccaacca cctggatatg gcgagccgcg aggttctgtc
     3841 cgaggccctg cggcaattcg agggcagcgt gatcttcatc agtcacgatc ggcacctcat
     3901 caacgccatc gccaccgagg tgatcgaagt gaaagccgga cgcctcaccc actacatcgg
     3961 tgactgggag tactaccagt ggaagtcggg tcgcgccggc gccgtcccgc ctaccgcgag
     4021 actcggcacc cggagcgagg cggccgggat cgaggacgcc gggtcttctg cagtgggact
     4081 cccggctgcg gacacgccgg gcgtgggctc gccttcatac cgggagcgca aggacatgca
     4141 gcgccggcac cggcagctcg agaaacggat catggctctg gaggatcgac agagggagct
     4201 gggcgggctg ctcgccgatc cggcccacgc ggccgactac gaactgctga tgagtgccag
     4261 cgccgaggcc actgagatca aggacgaact tgcgggcctc tatcccgaat gggaggtcgt
     4321 caccgaacgg gtggccgccc tggagtgagt cgtcggcgtg ggccttgccg cgtcgccgcg
     4381 ggtcttgcgc tgctggcggc ggcggttgtg acgcggtcag tggttgggac gcgtcagcgg
     4441 ttggcggcgc gtcagcggtt ggcgaccagc gtgggatagg ggttgattgc gcttccgcca
     4501 ccgggatgga tctcgaagtg caggtggact tcgccgcccc gcgcgttccc ggtgttccca
     4561 gcgtagccga tcacctggcc ggcagatacg cgtgtgcctg gtgtgatgct cgaggctatg
     4621 gacgtgaggt gcgcgtagta gtaggagttg ccgtcgccgc cgtcgagcca tatcgtgatg
     4681 ccgcccagac tccgttcggt ggggctggtc ctcttgatca ctccggcgac cacggccacc
     4741 acgtgagtgt tgcgggccgt catgatgtcg gtgccgcggt ggctgcggcc tccggaccgc
     4801 gcgaagcccc aatcgttgat gaagctgttc ggaccttgga cggggaaggc ccagcctgac
     4861 ccgctcacgg cgcggccggt ggtcgagccg gacgacccgg aggatcctga cgactgggcc
     4921 gccgccgccg cggccaggcg cgcggcctcc tcccgcttct tgcgtgcttc ttcttggagt
     4981 ctcgccacct tctcgcgaag cgtgttgtat tcgctcttgc tatcctcgag cgccttcatc
     5041 gcggcttcgt tggccttgtc gatctccgcg agctgctccg tttgcagtag tcgcttgtcg
     5101 gacaactccg cctcgaggga gtcgagtttg gcgacttgga gttccacctg cgacagtaca
     5161 tcgtcatcgc cctcgagcac ccgccccaac atggcgaggc ggttcaggat ccccgcgagg
     5221 gagtccccgt cgaagaagac cccaaggatg gcgagcccgc ccgcgtcgcc atccatgtac
     5281 atgccccgca ggcggtcgtt gaggcgcgca cgcatgatct cgaggtcggc cgcggcgcgg
     5341 tcggcttggg cttggacctc tgtcacgcgg tcctcggtcg tggcgagctc atcctgcgct
     5401 gtggcatacc gctgcgcgag cttgtcgagt tcggattggc gcgcgcgcag ctcggttttg
     5461 gctgcggcga gttcggactc ggtctgggcg agggtggtgg cggccagcgc cggatcaggt
     5521 gccaccgcca tgatggagac cgcgccgaaa gcaacgagca ctgcggctgc gagtgtggtg
     5581 acgattgaga cgcggaaccc gtggaggggg ccggctgtat gtgtgtgtcg cgcgttcccg
     5641 gaaagacggc acaaggtccg ccgggacgcc gcggagagat tccgaagcac catgacgacg
     5701 gagtgtagca gcttcgaggc ggtgcggcca agccggcgct gcatcgctgc cggggaacga
     5761 ggtggagcct ctgctatcct ggaggtcacc cgtgccgtcc gttcgcaagg ttcctgaagg
     5821 aggttctcca ttcccgctca gtcgaccgac cacagcatcg tcacgtctcg gccccccgtt
     5881 gccgtgaccg tgtctgcccg ccgaggagag ggcgcgcccg gcgttgacga ggtcatccgg
     5941 ctccgtgggg tctcactcgt tcgggacggt gtgcccctgt tgtccgccat cgactggtcg
     6001 gtcgcccgtg gggaacactg ggctgtgctt gggccgaacg gttcgggcaa gaccacgctg
     6061 ctgcgtctcg tggcgggcta cgtccaaccg acacaagggg cggcggaggt cctgggaggc
     6121 gtcttcggtc ggaccgacct tcgggtattg cgtcgccgca taggctgggt gagcccggcc
     6181 atggcggacc gccttcatcc gggagatccg gcattggacg tggtgctggg agggatcttc
     6241 gcgaccgtcg gcttgttcca cgagagcccc acccacgatg accgtgcgaa ggcgggggat
     6301 ctgctcgaag cgctcggatg cgggagtctg gggccacgca ggttcggcgt actctcgcag
     6361 ggtgagcggc agcgggtgct cctggctcgc gcgttgatca gcgatccttc acttctggtc
     6421 ctcgacgagc cgaccgcggg gctcgatgtg gccgcgcgcg aggacctcct ggagtcgctg
     6481 acggtgctcg cctccgcatc cgacggaccc acgatcctgt tggtcacgca ccacctcgaa
     6541 gagatcgtcc ccggcttctc gcacgccctg cttctgtcgg gcggccgcgt cctggcggcc
     6601 gggccgcggc agcgagtgct caccgcccct ctcgtgtcga gcgccttggg agtggatgtc
     6661 gaggtcgtcg agcgcgacgg gcggctttgg agcatcgtgc ggcgcaacgg ggggtgaccc
     6721 cgcctgggcc gcccggcgag gccttcggcc ggccgccgac gtcgtcgccg cgccccgccc
     6781 gcgggcgctc ggcggcatgc gtcacagtgg ccgcccgccg acgcccgggc ccgc
//
LOCUS       DASRMJ010000158         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_485842, whole genome shotgun sequence.
ACCESSION   DASRMJ010000158 DASRMJ010000000
VERSION     DASRMJ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485842"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2780
                     /locus_tag="VFD74_04440"
     CDS             <1..2780
                     /locus_tag="VFD74_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HZK48830.1"
                     /translation="DFWLGMGVDGLRLDAVPYLYEREGTTGENLPETHAYLKRLRRHV
                     DQNFKNRVLLAEANQWPEDAAEYFGNGDECNMAFHFPVMPRLFMAIRQEDRFPVVDIL
                     HQTPDIPENAQWALFLRNHDELTLEMVTDEDRDYMYRMYAGEPQARINLGIRHRLGPL
                     LHNDRRQIELMHGLLFSLPGTPVLYYGDEIGMGDNIYLGDRNGVRTPMQWSSDRNAGF
                     SRSNPQRLYLPVITDPEYHYEAVNVEAQQNNPHSLLWWVKRLIALRKQHQAFGRGSIE
                     FLFPENRKVLAFIRSWQDERILVVANLSRFTQYVALDLSRLAGTIPVELFGRAVFPEV
                     QGEGSYDFTLAPHAFHWLAMETPRVESEADVFDDETGPPALAVREAWSAVFEGKARVD
                     LENRLAKALKGRRWFQGKARSIVRVVIQETIPVPLAGKDEVHLVLAEVEYSEGEPETY
                     VLPLAYTADSEHARKDLGPSLIARLTVRRPGEADGAGLLFDAFGDASLSNELLDLVAR
                     RRRLKGSGGSLVGVPTKAFQNLRGADELPAPTLLRGEQTNTSVVYGNRLILKLFRHIE
                     EGVNPDLELGRFLTERTGFTSIAPVAGALEYRRSRQEPVTVAVLQGFVPNEGDAWLYT
                     LDSLGRFFERVLTMEGRRSAAPAGTRPLLELVQEEIPQDAADLVADYLESARLLGRRT
                     AELHAALASDTDDPAFTPEPFTPHYQRSLYQSVRGMVRQSFEILRRRRHLLAEDDRAL
                     ATQVLNHEGEVMARLYEALTKPLGALRTRTHGDYHLGQVLNSGRDFVIIDFEGEPARP
                     MSERRMKRSPLIDVAGMIRSFNYAATTALNMGTVRPEDVADLFPWAHYWYRLVSAAFL
                     GSYLEEAQNGLWLPRRPEALERLLETFLFEKAAYELRYELNNRPAWVGVPLRGLLELI
                     EERRSHSREEE"
     gene            2782..>3303
                     /locus_tag="VFD74_04445"
     CDS             2782..>3303
                     /locus_tag="VFD74_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HZK48831.1"
                     /translation="MTDPAPERHTLLTKDDLYLFNEGSHLRLYEKLGARPLTVEGAEG
                     THFAVWAPNAERVSVVGDFNGWNPETHLLQAREASGIWEAFLPGVGKGALYKYHIVSR
                     GSGYRVDKADPFGRMHEEPPHTASVVWDLEYEWGDQAWMVTRGGHNSLSAPIAIYEVH
                     LGSWMRDPADPSRL"
BASE COUNT          577 a         1151 c         1027 g          548 t
ORIGIN      
        1 tcgacttctg gctgggcatg ggcgtcgatg gtctacgcct ggacgccgtg ccctacctgt
       61 acgagcgcga gggcaccacc ggtgagaacc tgcccgagac gcacgcctac ctcaaacgcc
      121 tgcgccgcca cgtcgaccag aacttcaaga accgggtgct gctggccgag gccaaccagt
      181 ggcccgagga cgcggccgaa tacttcggca acggcgacga gtgcaacatg gccttccact
      241 ttccggtgat gcccaggctc ttcatggcca tccgccagga ggaccgcttc cccgtcgtcg
      301 atatcctgca ccagacgccg gacatccccg agaacgccca gtgggccctc ttcctgcgca
      361 accacgacga gctcaccttg gagatggtca ccgacgagga ccgcgactac atgtatcgca
      421 tgtacgccgg cgagcctcag gcccgcatca atctgggcat acgccaccgg ctggggccgc
      481 tcctgcacaa cgaccgccgc cagatcgagc tcatgcacgg cctgctcttc agcctgccgg
      541 gcacccccgt gctctactac ggcgacgaga tcgggatggg cgacaacatc tacctgggtg
      601 accgcaacgg cgtgcgcacc cccatgcagt ggagctccga ccgcaacgcc ggcttctcac
      661 gatccaaccc ccaacgcctc tatctgccgg ttatcaccga cccggagtac cactacgagg
      721 cggtcaacgt cgaagcccaa cagaacaacc cgcattcgct gctctggtgg gtgaagcgcc
      781 tcatcgcgtt gcgcaagcag catcaggcct tcgggcgcgg cagtatagag ttcttgttcc
      841 ccgagaaccg gaaggtgttg gctttcatcc ggtcttggca ggacgagcgg atcctggtgg
      901 tggcgaacct ctcccgcttc acccagtacg tcgcgctcga cctctcccgt cttgccggta
      961 cgatcccggt ggaactcttt ggtcgcgcgg tcttccccga ggtgcagggc gaaggctcat
     1021 acgatttcac cttggcaccg cacgccttcc actggctggc catggagacc ccccgcgtcg
     1081 agtccgaggc ggacgtgttc gacgacgaga ccgggccgcc cgctctggcc gtgcgcgaag
     1141 cctggtcggc ggtcttcgag gggaaggcgc gggtcgacct ggagaatcga ctggccaagg
     1201 cgctcaaggg acgtcgctgg ttccaaggaa aggcccgctc catcgtgcgc gtggtcatcc
     1261 aggagaccat ccccgtgccc ttggcgggga aggacgaagt gcatctggtg ctcgccgaag
     1321 tggagtactc ggaaggcgag cctgagacct acgtcctacc cctggcctac accgccgact
     1381 ccgagcacgc ccgcaaggac ctggggccca gcctgatcgc ccggctcacc gtgcgccgac
     1441 cgggagaggc cgacggtgcg ggcctgctct tcgacgcctt cggcgacgcc tctctatcga
     1501 acgagctgct ggatctggtc gcgcggcgcc ggcgactgaa gggatccggc ggatctctgg
     1561 tcggagtacc cacgaaggcc ttccagaacc tacgcggcgc cgacgagctg cccgccccta
     1621 cccttctacg gggcgagcag acgaacacct cggtggtcta cggcaaccga ctgatcttga
     1681 agctcttccg ccacatagag gagggcgtga accccgatct ggagctgggc cgctttctca
     1741 ccgaacgcac ggggttcact tccatcgccc cggttgccgg cgccttggag taccgccgga
     1801 gtcggcagga gcccgtgacc gtggccgttc tccagggctt cgtgccgaac gagggcgacg
     1861 cgtggctcta caccctggac tcgctgggcc gcttcttcga acgggtgctg accatggaag
     1921 gccggcggtc cgccgcgccg gccggcacgc gacccctcct cgaactggtt caggaggaga
     1981 tcccccaaga cgccgcggac ctggtggccg actatctgga gtccgcgcgc ctgctgggca
     2041 gacggaccgc cgagcttcac gccgctctgg cctcggacac ggatgatccg gcctttaccc
     2101 ccgagccctt caccccccac taccagcgtt cgctctacca gtcggtgcgg ggaatggtcc
     2161 gccagagctt cgagatcctg cgccgccgcc gtcatctgct cgccgaagac gatcgcgccc
     2221 ttgccaccca ggtgctcaac cacgagggcg aagtgatggc ccgcctgtac gaggccctca
     2281 ccaagcccct tggagccctg cgcacgcgca cccacggcga ctatcatctt ggtcaggtac
     2341 tgaactcggg gcgcgatttc gtcatcatcg atttcgaggg agaacccgca cgccccatga
     2401 gcgagcgacg tatgaagcgc tcgcccttga tcgacgtggc gggcatgatc cgttcgttca
     2461 actacgcagc cacgaccgca ctgaacatgg gaaccgtgcg gcccgaggac gtggccgatc
     2521 tcttcccgtg ggcgcactac tggtatcgcc tggtctcggc cgccttcctc ggttcgtacc
     2581 tcgaggaagc ccagaacgga ctgtggcttc ctcgccgccc cgaagccctc gagcgcctgc
     2641 tcgagacgtt cctgttcgaa aaggccgcct acgagcttcg gtacgagttg aacaaccggc
     2701 ccgcctgggt gggcgtcccc ctgcgagggc ttctggaact gatcgaagaa cggcggagcc
     2761 acagccgcga ggaagagtga catgaccgat cctgcgcccg aacgacacac cctgctcacc
     2821 aaagacgacc tctacctctt caacgagggg agccacctgc gactctacga gaagctgggc
     2881 gcccggcctt tgacggtcga gggcgcggaa ggcacccact tcgccgtctg ggctccgaat
     2941 gcggagaggg tctcggtcgt gggcgacttc aacggatgga atccggagac ccacctcctt
     3001 caagcccgtg aagcctcggg catctgggag gcgttcctgc ccggcgtagg caagggtgcg
     3061 ctatacaagt accacatcgt ctcccgcggc agcggctacc gggtggacaa ggccgatccc
     3121 ttcggtcgca tgcacgagga gccgccgcac accgcgtcgg tggtatggga tctcgagtac
     3181 gagtggggcg accaagcgtg gatggtgacg cggggtggcc acaactccct gagcgcgccg
     3241 atcgccatct acgaggtgca tctaggttcg tggatgcggg atccagccga tccttcccgc
     3301 ctg
//
LOCUS       DASRMJ010000159         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_12436, whole genome shotgun sequence.
ACCESSION   DASRMJ010000159 DASRMJ010000000
VERSION     DASRMJ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12436"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFD74_04450"
     CDS             complement(<1..201)
                     /locus_tag="VFD74_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HZK48832.1"
                     /translation="MSGSAPLLGAIADDVTGAADLCSTFVREGMRTVLTIGVVRDLDL
                     PAVDALVVALKSRTAPVAEAVGD"
     gene            complement(202..1653)
                     /locus_tag="VFD74_04455"
     CDS             complement(202..1653)
                     /locus_tag="VFD74_04455"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HZK48833.1"
                     /translation="MDGNKPISVPLSLRTIQTELLLPPKTVVLGTRQPTALASVPAAL
                     ATALGAPLGRPPLCEMGRQVLDRNQSASAVVVISDNTRPVPYKGPDGILWPIVESLLQ
                     SGFRPERIRILAASGTHRVMTVSEISSLIDDRVIAAGVGVTCHNAHDPSVLVAVGCSA
                     QGTTILMNREYVAADLKILTGLVEPHFMAGVSGGRKSVCPGLLGVQSVEEFHGAAMME
                     HPGTCNMNITDNLCHEYAIQVARMAPPDFVVNVTIRDDGKPVDVFAGDMDEVLETAFE
                     QLKSFVLLPIPHRFDIVVTHAGRGAINHYQAAKAATTGAQAVTQDGFLVVVADTTDPD
                     PIGNGTYREMLELMKKVGPTAFRQLIGSSDWTLVPDQWEVQMWAKVLDRVPEDHLFYY
                     SPQTRLSDYAILPCADPPAEGATTDVSEIVAAFIEQARQQATRALGRRPSIAYLSDGP
                     FGIPVEAVDAQAFLHESATVDLPAAEHLLGGEA"
     gene            complement(1975..2289)
                     /locus_tag="VFD74_04460"
     CDS             complement(1975..2289)
                     /locus_tag="VFD74_04460"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZK48834.1"
                     /translation="MRTGVRCAKASGLRNRPFRSYVMNDAWLELVLCGIDLLSWTRRL
                     LLTGTDLATCEPKRLRYRLLHVAGRIALHAGGVRLRLPRSWPWAQTLAAAFARLQALP
                     SG"
BASE COUNT          495 a          970 c         1063 g          507 t
ORIGIN      
        1 atcgccaacg gcctcggcga ccggtgccgt ccgtgacttc agtgcgacga cgagagcatc
       61 gaccgcgggc aggtcgaggt cgcggacgac gccgatggtc aggacggtgc gcattccttc
      121 tctcacgaac gtgctgcaga ggtcggcagc gcccgtcacg tcgtcggcga tcgcgccgag
      181 tagtggtgcg ctccctgaca ctcaggcctc gccacccagg aggtgctcgg cggccggcag
      241 atcgaccgtc gctgactcgt ggaggaatgc ttgggcgtcg accgcctcca ctgggatgcc
      301 gaaggggccg tcggacaggt aggcgatgct cggtcttctt cccagggcac gggtcgcttg
      361 ctgacgggct tgctcgatga aggcggcgac tatctccgac acgtcggtgg tagcgccctc
      421 cgcgggagga tcggcgcagg gtaagatggc gtagtccgac agcctcgtct gggggctata
      481 gtagaacagg tggtcttctg gcacgcggtc gaggaccttt gcccacatct ggacttccca
      541 ctggtcgggg accaaagtcc agtcgctgga tccgatcaat tgcctgaaag ccgtgggtcc
      601 caccttcttc atgagctcca gcatctctct gtaggttccg ttgccaatgg ggtccggatc
      661 tgtcgtatct gctacaacga cgaggaagcc atcctgcgtg accgcctgtg cccctgtggt
      721 tgccgctttg gccgcttggt agtggttgat cgccccccgc cctgcgtggg tgacaacgat
      781 gtcgaaccga tggggaatgg gcaggagtac aaagctcttg agctgttcga aagcagtctc
      841 gaggacctca tccatgtctc cagcgaagac gtccaccggt ttgccgtcgt ctcggatggt
      901 aacgttcacc acgaagtccg gaggggccat ccgggctacc tggatggcgt actcatggca
      961 gaggttgtcg gtaatgttca tgttgcacgt acccggatgc tccatcatgg ccgccccatg
     1021 gaactcttcc acactctgga ccccaagaag ccccgggcag acgcttttgc gacctccgga
     1081 gactccggcc atgaagtggg gctccaccag cccggtgagg atcttgaggt cggcggccac
     1141 gtattcccgg ttcatgagta tggtcgtgcc ctgggcggag cagccgacgg cgacgagcac
     1201 ggaaggatcg tgcgcattat ggcaggtgac gccgactccg gccgctatca cccggtcatc
     1261 aatgaggctg ctgatctcgg aaacggtcat cacgcggtgc gtgccggagg ccgccaagat
     1321 gcggatgcgt tcggggcgaa agcccgattg cagcagggat tcgacgatgg gccacaggat
     1381 gccgtcgggg cccttgtagg gcacggggcg ggtgttgtcg gagatcacca ccacggctga
     1441 agccgattga tttcggtcca ggacctgccg ccccatctcg cagagcggag ggcgaccgag
     1501 aggggcgccg agagcggtcg ccagagcggc gggcacgctg gcaagagccg tgggctggcg
     1561 ggtgccgagg accacggtct tcggcggcaa gagaagctca gtctggatcg tcctcaacga
     1621 aagcggcacc gatatcggtt tgttaccatc catgcgttga acctgctttc tgcttgccct
     1681 acgggacgga aggctgcggg acggaaggct actcttttct aattatgcac cggcgttcgt
     1741 ggcgtctggg cgtggtcttc cctggcctcg atccttcacg aacggtctcg cggcctcttc
     1801 tgaggacccc gtccggcccc tcccacgata gcgcatccgc gtgggcgcag gctaggaccg
     1861 cactggagcg gccgcggcgc cccgaggcgt cgtcgggagt gatccccgtc cctttcaagg
     1921 cctgctgaag ccggccccgt aagcgcctgc gtctgcggag cggcggtccg ctcgtcagcc
     1981 tgagggtaag gcttgcaggc gcgcgaaggc ggcggcgagg gtctgcgccc aaggccagga
     2041 gcgcggcagg cgcagcctca cgccgccggc gtggagcgcg atccggccgg cgacgtgcag
     2101 caggcggtag cgcaggcgct tgggctcgca ggtggccaga tcggtgccgg tgagcagcag
     2161 cctccttgtc caggacagca ggtcgatgcc gcagagcacg agctcgagcc aggcgtcgtt
     2221 catgacgtag gagcggaagg gccggttgcg cagccctgag gccttggcgc agcggacccc
     2281 agtcctcaat ggcggcgcgc cggcgatgca cctgttcgag gcagacgatg cgcctcccct
     2341 tctggttggt gagcatcacc tggaagcggt ggccttcggc gtcgctgaag gagagctgcg
     2401 cgccggggtg cgggcgctca cgacggcaga tcgcccgcgt gccctcgggc caggccgaga
     2461 gctccacatc gagctcggcc acccaggcgc cctcgcgcgg gccaccctca ctgtcgagcg
     2521 ccgggagcca ggcgtcctgc ggcacggcca ggcacgccgc gcacacgcgt tcggacagct
     2581 cgaaccccac cgtctgggcg atgaaacgag aaacgccccg ggaggtgtgg gccgggccgc
     2641 cccgccggcg ggcacgcagg acctcgcctg tgcctgcggc cacggcgagc aggggtggta
     2701 agccccgtac gtgggtgtag cagaagctgc tccccccggc tttcttcagg ccgtaggttt
     2761 cgcagatggt ggaatccagg tcgatggtga ggggttgagc gcccggtccg gcgccggcgc
     2821 cccacgcgcg ggcgagcaga tgcagcgaga cggcgtcgag ctgcagggcg tggccccagg
     2881 caaaggaccg caggaaggtg cccacggtgg aggggcccgc acctcgcagc cgagcacggc
     2941 ctcggccgaa cccgcccgca gcatgttcac atcgtcgctc gagtcccccc ggcaagcgcg
     3001 gagaagatga gggtcattcc cttcacgccc acgtt
//
LOCUS       DASRMJ010000160         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_454870, whole genome shotgun sequence.
ACCESSION   DASRMJ010000160 DASRMJ010000000
VERSION     DASRMJ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454870"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..863)
                     /locus_tag="VFD74_04465"
     CDS             complement(81..863)
                     /locus_tag="VFD74_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZK48835.1"
                     /translation="MSSNTVLSERKGSVCSITINRPDNFNALDLETARALSEAVASCH
                     DEEVRAVVITGAGRAFCSGGDLAHLGAGPDLGAALGDVLLHLNRAVIDIRLLAKPVVA
                     AVNGVAAGGGMGLALACDLRIVSEKAKFKQGFTSAALVPDTGWSLFAPLLSGLSLASE
                     MLLLDPLLTAEQARKYGIANKVVPADEFTERVESVVQSLAAGATLAFAEAKRHLNRAV
                     LPNLETHLEQERQAMIRAGRTADAREGLTAFGEKRAPVFQGR"
     gene            complement(959..>2802)
                     /locus_tag="VFD74_04470"
     CDS             complement(959..>2802)
                     /locus_tag="VFD74_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZK48836.1"
                     /translation="RSHWVEPLPPELTRRFLGGAGINAALALELLEPRADAFAPENCM
                     IFGLGPLVGTMVPGAGKGNVTARSPYGRFLGISGHGRFGALKYAGYDHLVITGRAERP
                     TVVSVADDRVTFLDATDLWGRDVFETTDRIWSQLGARTDVTCIGPAGENRVYDAALIT
                     NKYAAFARAGMGAVLGSKNCKAIAVSGTGGIRVADRKRFLKASRKLFDLIRSDRNLLE
                     WRRLGTLISLETFAEMGLYTKKNFQSAYDGGLLEHFDPDTFAGQIKEGDVACLGCPVG
                     CKHHVRVPDGNGGEVRMAVSCMNAVMQSFGTFCALEGWSEVIRCADQAARMGLDFMST
                     GSLISFAMELQQRGLLTPEDTGGLRLEWGDGEAMREMIHLIGTRQGFGERLAEGVVRA
                     AEQLGPETAPYAMHSKGLGMLYDPRVRLGSTEVFSQFTNVRGHISNVSVAMVERTPEQ
                     IRRYCEKIGLGREAVDRIVSPDGYNVGRLNKWTEDVCTAMEILGVCMFAPFQRLPLDL
                     WAEACSAATGIETDADDLVRASENLWHARRAYNLREGAGAADDTCPERFFSEPVAAGS
                     KSFAPLSESSFWDLVRAYYSERGWDEGKGEPPAAVLDELGLLEAFRR"
BASE COUNT          448 a          948 c          926 g          480 t
ORIGIN      
        1 ctctcaccca aagtctcgta ggcaatgccc ggcatcgcct cctgggagat ctcccgacac
       61 gcccaggcag tccggcgacc ctatcgtcct tggaacaccg gggcacgctt ctccccgaag
      121 gctgtgaggc cctcccgtgc gtccgcggtg cgacccgccc ggatcatggc ctgtcgctcc
      181 tgctccagat gggtctccag gttcggcagc actgcacggt tcaggtgccg cttggcctcg
      241 gcaaaggcca gcgtggcccc cgccgcgagg ctctgaacca cggactcgac tctctcggtg
      301 aactcgtcgg cggggactac cttgttggcg atgccgtact tgcgggcctg ttcggcagtg
      361 agcagcggat ccagtagcaa catctcgctg gccagcgaaa gtccgctgag gagaggggcg
      421 aacagactcc agccggtgtc cggcaccaga gcggcgctgg tgaagccctg cttgaacttg
      481 gccttttccg agacgatgcg caggtcgcac gccagcgcca gccccatgcc gccgcccgct
      541 gccacgccgt tgacggccgc caccaccggc ttggccagca ggcggatgtc gatgaccgcg
      601 cgattgaggt ggaggaggac atcgccgagg gctgccccga ggtctggacc ggcccccagg
      661 tgggccaggt cgccgccgga gcagaaagcc cggcccgcac cggtgatgac cacggcccgc
      721 acctcttcgt cgtgacaact ggccaccgcc tcgctcagtg cccgggcagt ctccaggtcc
      781 agggcgttga agttgtcggg tcggttgatg gtgatggagc agacgctgcc ttttcgttcg
      841 gagagtacgg tgttcgagct cattttgttt ctcctttccc cctcaagtgt gcaccgcggg
      901 tcagcagaga tcgattgagt ggtgacggtc cgggtcggtc gcggcggatc cgcggtcact
      961 atctgcggaa cgcctccaac agccccagtt cgtccagcac tgcggcgggc ggctccccct
     1021 tcccttcgtc ccatccgcgc tcggagtagt aggctctcac caagtcccag aaggaggact
     1081 cggagagcgg ggcaaagctc ttgcttccgg ccgcgaccgg ctcggagaag aatcgctcgg
     1141 gacaggtgtc gtccgcggct ccagcccctt cgcggagatt gtatgcccgg cgcgcgtgcc
     1201 agaggttctc ggaggctcgc accaggtcgt cggcgtcggt ctcgatgccg gttgccgccg
     1261 aacacgcttc ggcccacaag tccaggggaa ggcgctggaa gggggcgaac atgcacaccc
     1321 ccaggatctc catggcggtg cacacgtctt ccgtccactt gttgagccgc cccacgttgt
     1381 atccgtccgg ggagacgatg cggtccacgg cctcccgtcc caggccgatc ttctcgcagt
     1441 agcggcggat ctgttcgggg gtgcgctcca ccatcgcgac gctgacgttg gaaatgtggc
     1501 cgcgcacgtt ggtgaactgg gagaagacct cggtggagcc gaggcgcacg cggggatcgt
     1561 agagcatgcc cagacccttg ctatgcatgg cgtacggggc ggtctcaggt cccagctgtt
     1621 ccgcggcgcg cactaccccc tcggccaggc gttcaccgaa gccctgccgg gtgccgatga
     1681 ggtggatcat ttcgcgcatc gcttcgccgt cgccccactc cagccgcagg ccgcccgtat
     1741 cctccggggt gagcagtccg cgctgctgca gttccatggc gaaggagatc agggagccgg
     1801 tgctcatgaa gtccagaccc atgcgcgccg cctggtcggc gcagcggatc acctcggacc
     1861 agccctccag ggcgcagaag gtgccgaagc tctgcatcac ggcgttcatg caggagaccg
     1921 ccatgcgcac ctccccgccg ttcccgtccg gcacccgaac gtggtgcttg cagcccaccg
     1981 ggcagcccag gcaggccacg tccccctcct tgatctgccc ggcgaatgta tccgggtcga
     2041 agtgttccag gagcccccca tcgtacgcgg attggaagtt cttcttggtg tataacccca
     2101 tctccgcaaa ggtctccagg ctgatcaggg tgcccagccg gcgccattcg aggaggttgc
     2161 ggtcggaacg gatgaggtcg aacagcttgc ggctggcctt tagaaaacgt ttccgatcgg
     2221 ccactctgat gccgccggta ccggacacgg caatggcttt gcagttcttc gagcccagta
     2281 ccgcccccat gccggcccgg gcgaaggcgg cgtatttgtt ggtgatgagc gcagcgtcgt
     2341 agaccctatt ctctccggcc ggcccgatgc aggtaacgtc ggtgcgcgcg ccgagctggg
     2401 accagatccg gtcggtggtc tcgaagacgt cccgccccca caggtcggtg gcgtccagga
     2461 aggtgacccg gtcgtcggcc accgacacta ccgtagggcg ttccgcccgc ccggtaatca
     2521 ccagatggtc gtagccagcg tacttcaggg ccccgaagcg gccgtggccg gagattccca
     2581 ggaatcgccc gtagggggag cgggcggtga cgttgccctt gcccgccccg ggcaccatag
     2641 ttccaacgag ggggcccagg ccgaagatca tgcagttctc gggagcgaag gcatcggctc
     2701 ggggttcgag caactccaga gccaaggcgg cgttgatgcc ggcccccccg aggaaacggc
     2761 gggtcagctc cgggggcagg ggctcgaccc agtgggaccg ag
//
LOCUS       DASRMJ010000161         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_302087, whole genome shotgun sequence.
ACCESSION   DASRMJ010000161 DASRMJ010000000
VERSION     DASRMJ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302087"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1612
                     /locus_tag="VFD74_04475"
     CDS             119..1612
                     /locus_tag="VFD74_04475"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HZK48837.1"
                     /translation="MPPAHTSRRPPRLSKSRFQTGLQCYKKLWLKCFEPSLADPIDEV
                     QQAIFDQGHRVGDLARERYPGGVLVAEDYRHTAAALAKTAELIASGASCLYEAAFEYD
                     GVLVRADVIVRRLDGSWELVEVKSTSKAKLEHHTDVAIQLYVLEGAGLSVAAAGVLHL
                     DTSYVYAGGAYDLIELYSLADVTERARAILPNIPGLLADMKAMLSSDCPDVRVGKHCT
                     QPYDCDFRGYCHSHLPEFPVTGLPRIGDALLDALLADGILSMRDVPPDYPGLTDAQAA
                     ACELVRDGRPHFAAGLVEQLGALGQPLNFLDFETVASALPLYAGTRPYQALPVQWSCH
                     TLHADGALEHREFLHEERSDPRRSFALSLPAGLPPQGRVVVYSSYENTVLTSLSGDLP
                     DLSARIADIQDRLFDLLPLVRRHVRHPDILGHASLKYVLPALVDDLSYDDLAVQNGAV
                     AGLRYAAALADGFPEAERRQLFADLRAYCATDTLALVRLFQTLKAQD"
     gene            complement(1713..2018)
                     /locus_tag="VFD74_04480"
     CDS             complement(1713..2018)
                     /locus_tag="VFD74_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HZK48838.1"
                     /translation="MKETMLDPIHPGVILLEEFLEPLGISQYRLAKSISVPPRRINEI
                     VHGKRSITADTALRLAKFFGDTERFWLNLQVRYDLEVEKGRLSGVLERDVSVYRAAV"
     gene            complement(2031..2312)
                     /locus_tag="VFD74_04485"
     CDS             complement(2031..2312)
                     /locus_tag="VFD74_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZK48839.1"
                     /translation="MIRSFRDADTERVFDRRGSTRLPPDVCRTALKKLLMIDAAESLL
                     DLRVPPGNRLERLSGDRRERYSIRINDQWRICFRWKDGDALDVEIVDYH"
     gene            complement(2617..2688)
                     /locus_tag="VFD74_04490"
     tRNA            complement(2617..2688)
                     /locus_tag="VFD74_04490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2654..2656),aa:Thr,seq:tgt)
BASE COUNT          537 a         1019 c          874 g          494 t
ORIGIN      
        1 tgcgtaggga gagcgcaact caaagacccg gagtgggcac ggaaagcgct ggcaagcttg
       61 taggtgcccc cgaggatctc gacggcattc catccgcccg acctggtagc ctatgttcat
      121 gcctcccgcg catacaagtc gccggccccc acgcctgtcg aagtcgcgct tccagaccgg
      181 tcttcagtgt tacaagaagc tgtggctcaa gtgcttcgaa ccgagcctcg ccgatccgat
      241 cgacgaagtg cagcaggcca tcttcgacca gggccaccgc gtgggcgatc tcgctcgaga
      301 gcgttacccc ggcggcgtcc tggtagccga ggactacagg cacacggctg cggctttggc
      361 caagaccgcc gagctgatcg ccagcggagc ctcctgcctg tacgaggccg ccttcgagta
      421 cgacggggtc ctggtgcggg ccgacgtgat cgtcagacgg cttgacggca gctgggaact
      481 tgtggaggtc aagtccacga gcaaggccaa gctcgaacac cacaccgatg tcgccatcca
      541 actgtacgtg ctcgagggcg cgggcctgtc cgtggcggcc gcgggcgtgt tgcacctcga
      601 caccagctac gtctacgccg gcggggcata cgatctgatt gagctctact ccctggcgga
      661 cgtcaccgag agggctcggg ccatccttcc caacatcccc ggcctactcg ccgatatgaa
      721 ggccatgctt tcgtccgact gccccgacgt ccgcgtgggg aagcactgca cgcagccgta
      781 cgactgcgac ttccgcggat actgccacag ccacttgccg gagttcccgg tcaccgggtt
      841 gccccgcatc ggcgacgcgc tgctcgatgc gctgctcgcc gacgggatac tctccatgcg
      901 cgacgtgccg cccgactacc cgggactcac cgacgcccaa gcggccgcct gtgagctcgt
      961 ccgcgacggc cggccccact tcgccgccgg cttggtggag cagctcgggg cgctcggaca
     1021 acccctgaac ttcctcgact tcgaaacggt ggcgtccgcc cttccgctgt acgccggcac
     1081 gcgcccctac caggccctgc cggtgcagtg gtcctgccac acgctccacg ccgacggcgc
     1141 actggagcac cgcgagttcc ttcacgagga gcgcagcgat ccccgccgca gcttcgctct
     1201 gagtctgccg gccgggctgc cgccgcaggg gcgtgtcgtg gtctactcca gctacgagaa
     1261 cacggtgctc acgtccctct ccggggacct ccccgacctg tcggcccgca tcgcggacat
     1321 ccaggaccgg ctcttcgatc tgctccctct cgtgcgccgc cacgtgcgac acccggacat
     1381 tcttgggcac gcctcgctca agtacgtgct tccggcgctg gtggacgacc tctcctacga
     1441 cgacctggct gtgcagaacg gcgccgtcgc cggtctgcgc tatgctgcgg ccctggccga
     1501 cggattccca gaggccgagc gccgacaact cttcgccgac cttcgggcct actgcgccac
     1561 cgacacgctg gcgttggtgc gcctgttcca gactttgaaa gcccaagact gagtcacgca
     1621 gtcggcggga agtcgggccg gaatgaccga cacagacgcc gaaacggggc aacggagcgc
     1681 tgatagccgc caccctccac ggcatcgccg cactacacgg cagccctgta cacggaaacg
     1741 tcgcgctcca gcacgcccga gagacgccct ttctcgactt cgaggtcata cctgacctgc
     1801 aggttcaacc agaatctctc cgtatccccg aagaacttcg ccaggcgaag cgccgtgtcg
     1861 gcagtgatcg accgtttgcc gtgcacgatc tcattgatcc gccgcggggg cacactgatg
     1921 ctcttggcca gccggtactg gctgataccc aagggttcaa ggaactcttc cagaaggatc
     1981 actccagggt ggatggggtc caacatcgtc tccttcatcg catacctccc tcagtgatag
     2041 tcgacgatct cgacatcaag cgcgtcgccg tccttccagc ggaagcagat cctccactga
     2101 tcgttgatcc ggatactgta ccgttccctt cgatctccgg agagtctctc gagtctgttg
     2161 cccggcggca ctcgcagatc gagcaaggac tcagccgcgt cgatcatcaa gagcttcttc
     2221 aaggcggtgc gacatacgtc tggcggaagc ctcgtcgatc ctcggcgatc gaagacccgc
     2281 tcggtgtcag catctcggaa gctccggatc atgacgccat agtaacgctt ggcgttatta
     2341 gtatcaagga tagaggcggc tgcggttcca caggctctcc acaatgacga gggacggccc
     2401 ggcgaccgcc aacataccgg ccagatgctc cccgcaatga gcgagcgcgg tagcgagtga
     2461 cggtgacggt gacggtgacg gtgacggtga cgaagatatc gccattcccg gtgtcggagc
     2521 atgggacttt gagaggatcc tggaggcgac ggcgccccgt ctgggttccg ccccgtcgcc
     2581 tcgacaaccg gcgcaagtga tcagcaaaga ccagaaagcc ggcgaaggga atcgaacccc
     2641 caacctgctg attacaaatc agctgctcta ccattgagcc acgccggcga gcgttgccgg
     2701 tagattgtac gggtctcggg ccgaagtgac aaaggtcgaa gcaacggtgc cgaggcccct
     2761 accggtcgtc aggcggcgac cccccgtcgc caccgccaag tcccaagccc gaggatcacc
     2821 gcgccgaggc acagatacgc cgcctgcagc cagtgatagt cgagcatcac catctccacc
     2881 actatccagc cgacgagcac gacgcccacg accgtgtgac ctac
//
LOCUS       DASRMJ010000162         4892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_193704, whole genome shotgun sequence.
ACCESSION   DASRMJ010000162 DASRMJ010000000
VERSION     DASRMJ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193704"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFD74_04495"
     CDS             complement(<1..461)
                     /locus_tag="VFD74_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48840.1"
                     /translation="MNAVLEALATLRLLRVPGLPTVPRLLGLASLRHPRLVPRALRWK
                     AASRGLGLVVARLVAWEGDPSMAETSSGVGAGLQSIGEEQGRAVRAQLGYGRDPRECA
                     RAVALANRLFDIDASVVATGPDEARVVTPGCRWSREPWWGPAPCGAFSRYEL"
     gene            complement(499..1587)
                     /locus_tag="VFD74_04500"
     CDS             complement(499..1587)
                     /locus_tag="VFD74_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZK48841.1"
                     /translation="MKHMDELLRLMVEAGASDLHVKAGSPPMMRVDGELRPFELPALD
                     PEDTKDYAASLMSDKQIRRFSETNELDFAYSAPQIGRFRVNVYRQRGSISVAMRQVAA
                     VIPTFEELLLPAAVEKLSLEPRGMLLVTGTTGSGKTTTLAAMVDHINTNLRRHIVTIE
                     DPIEILHRDKRSIINQREVGLDTESYSSALKYVLRQDPDSILIGEMRDLETVRTALTA
                     AQTGHFVMSTLHTIDATETINRIIDFFPPYQQKQVRIMLAGSLRGIISQRLLPHVNAV
                     GRVPAVEVLVMTGRVRDFILDPAQTHNIETVIKEGEYYGMQSFDQSLLSLYERGLTTL
                     HDAVAAATSPHDFKLLVQSRGHDISLAD"
     gene            complement(1651..2676)
                     /locus_tag="VFD74_04505"
     CDS             complement(1651..2676)
                     /locus_tag="VFD74_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZK48842.1"
                     /translation="MATADVYFHPIFLEHDTGDHPECAERLVVARRRLLQSGMGIEWI
                     EPTPAEVEAVARVHDSAYIRAVHRMADAGGGSLDPDTVVSPLSYSAAMYAAGAGIQAV
                     ERALHGGRRSFLLVRPPGHHARREWAMGFCLFNNVAIAAAHALEELGVERVLIVDWDV
                     HHGNGTQEAFYDDPRVLYCSFHLGRHFPGTGSMDEIGVGRGLGYTANLPLMHGAGDGA
                     ASAFFSSVIGPLAEAFDPQLVLVSAGYDSGEGDPLGGLRLTREAFRWMSGTLGGMCAR
                     SGAAGPVCFLEGGYDTDVLASGIEATIEGLIEGPGEMSAQPTGAEAAAVETLTRRLSA
                     HWAGVLT"
     gene            complement(2693..3865)
                     /locus_tag="VFD74_04510"
     CDS             complement(2693..3865)
                     /locus_tag="VFD74_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HZK48843.1"
                     /translation="MFKPLDGCKCERSWRPPISGHTHLAGRRFLFILLLVILASLMFV
                     PSVSADPDAVAKAKIEAEKLRAQVQDLDDRLESAREDYQYAKIQLDEIDGAIVANQER
                     LDRSERDLEVAYERLSTRVVSIYQNGRVGLLDALFGAQSLNDFVNRLHLLTLVGAEDS
                     EVLKQVEAYRDDVAARKEQLAADQARQKELVAEAAAAGAQIEQRLAERKQLLAGKEQQ
                     VAQLEREEEERQRKLAEEVAEAVRQVAEAARLAAIAANATTHSTTPVTVPSHDVPASS
                     YGNPLVGVAMRYIGVPYVWGGISPSGFDCSGLVAYSFAQLGVSVPHSSRALFNMGVPV
                     AREDLQPGDLVFFGSPIHHVGIYVGGGNMVHAPYTGANVRVNTIDRSNYTGARRGL"
     gene            complement(3906..>4892)
                     /locus_tag="VFD74_04515"
     CDS             complement(3906..>4892)
                     /locus_tag="VFD74_04515"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZK48844.1"
                     /translation="GREGADGHFGPNTERAVREFQQRRLLLSDGVVGENTWTELVEAG
                     HQIGERLLYLRAPHMRGDDVRGLQRRLNELGFDSGPEDGIFGPLTDAGFADFQRNAGI
                     SMDGIVGESTLAHLRRIHMAAVGKGDRKIPDRMNGYVGRADMSGLTVSIDPAHGGSDA
                     GAVGVGSLAESAVNLALARDLATLLEEEGAEVYLVRSGNRTLGLYERTTLADSYGADL
                     QITLHHGYHAHPAARGAAAFYFANGSYFSEAGKRLAGYMVGALVADLGRVDLHPHGCN
                     YACLREPQGLAVMVEPGVITHPEEGAELATVEGVRSEAGCIVRAIRAYLERL"
BASE COUNT          832 a         1673 c         1564 g          823 t
ORIGIN      
        1 agctcatacc gggaaaaggc cccgcatggg gcggggcccc accagggctc tcgcgaccac
       61 cgacagcccg gcgtcaccac ccgagcctcg tcgggcccgg tcgccacgac cgatgcgtcg
      121 atgtcgaaca ggcggttggc caaagccaca gcgcgcgcgc actcacgcgg gtctcgaccg
      181 taaccgagtt gggcccggac ggccctaccc tgctcctcgc cgatggattg gagcccggcc
      241 cccaccccgg agctcgtctc ggccatcgag gggtcgccct cccaagccac aaggcgggcc
      301 acgaccagac cgagaccgcg agaggccgcc ttccaacgca gcgcgcgcgg caccaacctg
      361 ggatggcgca gcgaggccag ccccaagaga cgaggcacgg tcgggaggcc gggcacgcga
      421 agcaggcgaa gcgttgccaa tgcctcaagg acggcgttca agttgcccgc gcgccgcggc
      481 gtccgcactt cgactgcctc agtcggcgag cgagatgtcg tggccgcgtg actgcaccag
      541 cagcttaaaa tcatgcggac tggtggccgc cgcgacggca tcgtgcaggg tcgtcaggcc
      601 ccgctcgtac agggagagca ggctctgatc gaacgactgc atgccgtagt attcgccctc
      661 cttgatcacc gtctcgatgt tgtgagtctg ggcgggatcc aggatgaagt cgcgcactcg
      721 acccgtcatg acgagcactt ccaccgcagg cacccggccc acggcgttca cgtgaggcag
      781 aagccgctgc gagatgatgc cgcggagtga acccgcgagc atgatgcgca cctgcttctg
      841 ctgatacgga gggaagaagt cgatgatgcg gttgatggtc tcggtggcgt cgatggtgtg
      901 gagcgtgctc atgacgaagt gcccggtctg ggcggcggtg agtgcggtgc gaaccgtctc
      961 cagatcgcgc atctcaccga tcaggatgct gtcgggatct tggcgcagca cgtacttcag
     1021 agccgaggag tacgactcgg tgtccaggcc gacctcgcgc tggttgataa tcgaccgctt
     1081 gtcgcggtga aggatctcga tagggtcttc aatggtcact atgtgacggc gaaggttcgt
     1141 gttgatgtgg tcgaccatcg ctgcgagcgt cgtggtcttc cccgagccgg tggtgcccgt
     1201 gacgagcagc atgccgcgag gctccaagga cagcttctcc accgctgccg gaaggagcag
     1261 ttcttcgaac gtgggtatca ccgcggccac ctgccgcatg gcgacggaga tgctcccccg
     1321 ttgtcggtag acgttcacgc ggaagcgtcc gatctgagga gcgctgtagg caaagtccag
     1381 ctcgttggtc tcgctgaagc gtcggatctg cttgtcgctc atgagcgatg cggcgtagtc
     1441 cttcgtgtcc tccgggtcga gcgcaggcag ctcgaagggt cggagttcac cgtccacacg
     1501 catcatcggc gggctcccgg ccttcacgtg caggtcggaa gcgcccgctt ccaccatgag
     1561 tcgcagcagc tcgtccatat gcttcatgtc agtcctccgc ctcagtgaac gcatcagacg
     1621 cgggccggtc gcgccacaac tggatcgact tcacgtcagc acccccgccc aatgtgccga
     1681 caaccgcctc gtcagagtct ccaccgccgc cgcctccgcg cctgttggtt gcgccgacat
     1741 ctccccgggc ccctcgatca acccctcgat ggtcgcctcg atccccgagg ccagcacgtc
     1801 ggtgtcatac cctccctcga ggaagcacac cggacccgcc gctcctgacc gagcgcacat
     1861 accgccaagc gtgccggaca tccagcggaa ggcctctcgg gtgagtcgaa ggccgccaag
     1921 cggatcaccc tcccccgaat cataccccgc cgacaccagg acgagttgcg gatcgaacgc
     1981 ctcggccagc ggtccgatga cactcgagaa gaatgcggag gccgccccat cgcccgctcc
     2041 gtgcatcagc ggaaggttcg cggtgtaccc gagccctcgt ccgaccccga tctcgtccat
     2101 cgagcccgtt cccgggaagt gccttcccag gtggaagctg cagtacagca cccgcgggtc
     2161 gtcgtagaag gcctcctggg tcccgttccc gtggtgcacg tcccaatcga ctatcaacac
     2221 cctctccacg ccgagttcct caagagcgtg ggcggcggcg atggcgacgt tgttgaacag
     2281 acagaagccc atggcccact cgcgccgggc gtggtgaccg ggtggcctta ccaagaggaa
     2341 cgagcggcgg ccgccatgaa gagcccgctc caccgcctgg atcccggcgc cggcggcgta
     2401 catggcggcc gaataggaga gcggcgatac tacggtgtca gggtcgaggc tcccgccacc
     2461 tgcgtcggcc atccgatgca cggcgcggat gtacgcgctg tcgtgcactc gggcgaccgc
     2521 ctcgacttcc gccggggtcg gttcgatcca ctcgatgccc atacccgact gcagcagcct
     2581 cctgcgggcg acgaccaaac gctcggcgca ctcagggtga tccccggtat cgtgttctag
     2641 gaagatcggg tgaaagtaga cgtccgccgt ggccacgaca gacaatgctc cgctaaaggc
     2701 cccggcgcgc gccggtgtag ttgctgcgat cgatggtgtt gaccctcacg ttcgcccccg
     2761 tgtagggagc gtgcaccatg ttgcccccgc ccacgtatat gcccacgtga tggataggcg
     2821 acccgaagaa gacgaggtcc ccgggctgca ggtcttcgcg ggccacggga acgcccatgt
     2881 tgaagagcgc tcgactcgaa tgaggcaccg acacgcccaa ctgcgcgaag gagtaggcca
     2941 cgagacccga gcagtcgaag ccgctcgggg atatcccgcc ccagacgtat gggactccga
     3001 tgtatcgcat ggccactccg accagcggat tgccatagga ggacgccggc acatcatgtg
     3061 atgggacggt caccggcgtc gtggagtgcg tggtggcatt cgcagcgatg gccgccagcc
     3121 gcgccgcttc ggcaacctgt cgaaccgctt cggcaacctc ttccgcgagc ttccgctggc
     3181 gctcctcttc ttcccgttcc agttgggcga cctgctgctc cttgccggcc aggagctgct
     3241 tgcgttccgc cagcctctgc tcgatctggg cgcccgccgc ggcggcttca gccaccagtt
     3301 ccttctgtcg tgcttggtcg gccgccagct gctccttgcg cgccgccacg tcgtcgcgat
     3361 acgcctcgac ctgcttcaag acctcgctgt cctcggcgcc caccagcgtg agcaggtgga
     3421 ggcgattcac gaagtcgttg agcgattggg ctccgaacaa ggcgtcgagc agacccactc
     3481 gcccgttctg gtagatgctc acgacccggg tgctcagtcg ctcgtaggcg acctcgaggt
     3541 cacgctccga gcggtcgagg cgttcctggt tcgcgacgat ggcgccgtcg atctcgtcga
     3601 gttggatctt ggcgtattgg tagtcctcgc gggcggactc gaggcgatcg tcgaggtcct
     3661 gcacctgggc tcggagcttc tcggcttcga tcttcgcttt ggcgacggcg tcaggatcgg
     3721 cggagaccga ggggacgaac ataagcgatg cgaggatgac cagcaggagg atgaaaagaa
     3781 accgtctgcc ggcaaggtgg gtgtgaccgc taatgggagg cctccaggat cgttcacact
     3841 tgcagccgtc gagcggcttg aacacgaaag gactctacca cggcgccgcc cgaacggcca
     3901 aacgtctaga gacgttcgag gtaggcgcgt atcgctctca cgatgcatcc ggcttcgctc
     3961 cgcacaccct ccacggtggc aagctccgca ccttcctcgg ggtgcgtgat cactccgggc
     4021 tccaccatca ccgcaagccc ctgcggctcc cggagacagg cgtagttgca gccgtgagga
     4081 tgcagatcga ctcgtccgag atctgccacc agagccccga ccatgtagcc ggcgagacgc
     4141 ttcccggctt cgctgaagta ggaaccgttc gcgaagtaga aggccgcggc gccccgcgcc
     4201 gccggatggg cgtggtatcc atggtgaagc gtaatctgca ggtccgcgcc gtacgaatcg
     4261 gccagagtcg tgcgttcgta caggccgagg gtccggttcc cgctcctcac gaggtacacc
     4321 tccgctccct cctcctcaag caaggtggcg agatctcgtg ccagcgcgag gttgaccgcg
     4381 ctctccgcga gcgaccccac ccccacggca ccggcatcac tgccgccgtg agccgggtcg
     4441 atgctcacgg tgagccccga catgtcggcc cgacccacgt agccgttcat gcgatcgggg
     4501 atcttcctgt cgcccttccc caccgccgcc atgtggatgc gtcgcagatg ggccagcgtg
     4561 gactcgccca cgatgccgtc catggagatg cccgcgttcc gctggaaatc ggcgaaaccg
     4621 gcgtcggtca gcggcccgaa gatgccgtct tccggacccg agtcgaagcc gagttcgttc
     4681 aggcggcgtt ggagaccgcg gacatcatcc ccgcgcatgt ggggggcccg caggtagagc
     4741 agccgctcgc cgatctggtg accggcctcc accagttccg tccacgtgtt ctcgcccacg
     4801 actccgtcgg agagcaacag tctgcgctgc tggaactcgc gcaccgcgcg ctcggtgttg
     4861 ggcccgaagt gcccgtcggc gccctctcgg cc
//
LOCUS       DASRMJ010000163         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_302133, whole genome shotgun sequence.
ACCESSION   DASRMJ010000163 DASRMJ010000000
VERSION     DASRMJ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302133"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFD74_04520"
     CDS             complement(<1..257)
                     /locus_tag="VFD74_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyryl-CoA:acetate CoA-transferase"
                     /protein_id="HZK48845.1"
                     /translation="MNGHVTEYREKCVSADEAMKAVRSGDWIEYSWAASQACLLDEAL
                     AKRTEELHDVNFRGAVLLKPMACFERDPKGEHFTWNSWHAGT"
     gene            881..2020
                     /locus_tag="VFD74_04525"
     CDS             881..2020
                     /locus_tag="VFD74_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZK48846.1"
                     /translation="MDFALSQDHNVLRDTVRDFVQKEVKPLAISIDQEHKIPDELIAK
                     MSEMGLLGCFLPEEYGGAGLDILSYVLVVEEVSKACASSGVLISAHSSLGCAPIYQFG
                     TEEQKKKYLPDMATGRKIGCILLTEPDVGSDVAGMTTMYRREGDEFVITGGKTFITNG
                     ACLGTGIVFATHDKSLKHKGISVFIIDLKSPGVTVLKNEDKLGIRGSYTTAFALDDVH
                     VPVANLLAEEGQGFKVAMETLNGGRIGIASQALGIAEGAYGLALAYAKERKQFGAPIS
                     KLQAIQFKLADMYLRIEASRLLVYKAAWMKDNKMNYAMDSAMCKLFAAETAMYVTKEA
                     VQIHGGYGYIVEYEVERMYRDAKITEIYEGTSEVQRVVIAKALLS"
     gene            2049..>3530
                     /locus_tag="VFD74_04530"
     CDS             2049..>3530
                     /locus_tag="VFD74_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZK48847.1"
                     /translation="MEISREIYWNVGHVVVLPMYLFAFAAIAVFIWGFYRRTSTYRLG
                     KGLNRFDHLPQRHWLVARNALVQLRVLKVLLPGSLHSLFFWGFGVLFIGTLLVMAQSD
                     FFDLLFHIKFLQGGFYEFFSLALDLAGLVALLMLGGLLVRRFIVRPKGLETIRDDYLV
                     HALLFAILLTGFVIEGVRMAATEIGSNPDLARFSPVGYLFAEAFRGLSASQLESLHKV
                     LWWVHFFLAVGFIAAIPFTKLRHIFTATANYFFSDLSPKGTLATIDLEDESAEQFGAA
                     TVSDLTWKDIFDADACTSCKRCQDRCPAWSTDKPLSPMKVVQQIGELAFSAPEANLCE
                     TVTSDVLWSCTTCRACQEICPVSIEHVNKIIEMRRNLSLMEGAFAGDEVRTAINNFEV
                     NGNPFGLAYASRGEWAEGLDVQLMEEGADVDILYFAGCYASFDQRNQVVARSFVKICN
                     SAGVKVGILGKEEKCCGEPARKLGNEYLYQTMAQENIETIKVYG"
BASE COUNT          637 a         1178 c         1059 g          656 t
ORIGIN      
        1 gtaccagcgt gccagctgtt ccaggtgaaa tgctcgccct tcgggtcacg ctcgaagcag
       61 gccatcggct tcagcaggac ggcgccgcgg aaattcacgt cgtgcagttc ttccgtgcgc
      121 tttgccaagg cttcatccag cagacatgcc tgactcgccg cccagctgta ctcgatccag
      181 tcgccactcc gtaccgcttt catagcttca tcagcagaga cgcacttctc gcgatactca
      241 gtaacatggc cattcatgtc gatgaaaccc ccgccttccc tttccgccca agatgcgttc
      301 ccggcctttc agccgttgcg tccgcccata gtcccgttcc ctggctccgg gttcgctggg
      361 ccgtaggacc gcaatcgaag cttcggagtc aagtgtcatg tggggactgc cgggtgacac
      421 atgttagcgc aaggacgact ccaagccaat cacgaatcaa gcgggtggcc cggccttcag
      481 cagccctccg ggaatccgtc gttcaggtgt ccgtcgcggc gctcggacct tgacgcgcga
      541 gcgacgcgat tttccggggt cgagctgcat ctgtcgtggt gcttctgagc ccccaggttg
      601 tgccgcagaa cacgattctg atggttgtgc cgcagagcac ggttctgata tatctcgcga
      661 ggccattggt ggtaccgtcc acaccatcag cagccgctgg atttgtgatg gtcatttgct
      721 tgcagtcact tacctgattc gcggtcgccc ttacggccag gcggcggcga gtgaccgcct
      781 ggggccgaag acggcgggac gacctgcgag gggtgtatga gcggtcggaa agaggggtct
      841 ccgtgaggga gaagtgccga gacagagtgg ggaggtcaac atggatttcg cgctgagtca
      901 agaccacaac gtgctgaggg acacggtccg cgacttcgta cagaaggagg tcaagccgct
      961 tgccatcagc atcgaccagg agcacaagat cccagacgag ctgatcgcga agatgagcga
     1021 gatggggctc ctggggtgct tcctccccga ggagtacggc ggcgccggcc tggacatcct
     1081 ctcgtatgtc ctcgtggtcg aggaggtctc caaggcctgc gcctcgagtg gtgtgctgat
     1141 ctccgcgcac agttccctgg gctgcgctcc gatctaccag ttcggcactg aggagcagaa
     1201 gaagaagtac ctcccggaca tggcgactgg gcgcaagatc ggctgtattc tactcacgga
     1261 accagacgtg ggcagcgacg tggccggcat gaccaccatg taccggcgcg aaggcgacga
     1321 gttcgtcatc accggcggca agaccttcat caccaacggt gcctgccttg gcaccggcat
     1381 cgtctttgcg acccacgaca agagcctgaa gcacaagggc atctccgtct tcatcatcga
     1441 cctgaagtcc ccgggcgtga cggtgctcaa gaacgaggac aagttgggta tccgcggcag
     1501 ctacacgacc gccttcgccc tggacgacgt gcacgtgccc gtcgccaacc tcctcgctga
     1561 ggagggccag gggttcaagg tcgcgatgga gaccctgaac ggtggccgca tcggtatcgc
     1621 gtcgcaggcc ttgggcatcg ccgagggcgc ctacgggctt gctctcgcct acgcgaagga
     1681 gcgcaagcag ttcggcgccc ccatctccaa gctgcaggcg atccagttca agctggccga
     1741 catgtacctc cgcattgagg ccagccgcct gctcgtttac aaggccgcct ggatgaaaga
     1801 caataagatg aactacgcca tggactcggc gatgtgcaag ctcttcgccg ccgagacggc
     1861 tatgtacgtc accaaagagg ctgtgcagat ccacggcggc tacggctaca tcgtcgagta
     1921 cgaagtcgag cgcatgtacc gggacgccaa gatcaccgag atctacgagg gcaccagcga
     1981 ggtgcagcgc gtggtgatcg ccaaggccct cctttcgtag cgacgggcag agaccggaag
     2041 caacgcctgt ggaaataagc agagagatct actggaacgt cggtcatgtg gtcgtcctgc
     2101 ccatgtacct gttcgcgttc gcggccatcg ccgtcttcat ctggggcttc taccgacgca
     2161 cctccaccta ccgtctcggc aagggcctca accgcttcga ccacctgccg cagcgccact
     2221 ggctcgttgc gaggaacgcc ctcgtgcagc tgcgcgtcct caaggtgctc ctcccgggga
     2281 gcctgcacag cctcttcttc tggggcttcg gggtgctctt catcggcacc ctcctggtca
     2341 tggcccagtc cgacttcttc gacctgttgt tccacatcaa gttcctccag gggggcttct
     2401 acgagttctt ctccctggcc ctcgacctgg ccgggctggt cgccctgctc atgctcggcg
     2461 gcctcctcgt gcggcgcttc atcgtgcgcc ccaagggcct tgagacgatc cgcgacgact
     2521 acctggtcca cgccctgctg ttcgccatcc tgctcaccgg cttcgtcatc gagggcgtgc
     2581 gcatggccgc cacggagatc ggctccaacc ccgacctggc gcgcttctcg cccgtcggct
     2641 accttttcgc cgaggccttc cgcggcctct cggcgtcgca gctcgagtcg ctgcacaagg
     2701 tcctctggtg ggtgcacttc ttcctcgccg tgggcttcat cgccgccatc cccttcacca
     2761 agctgcgcca catcttcacg gccaccgcca actacttctt cagcgacctc tcccccaagg
     2821 gcaccctggc caccatcgac ctcgaggacg agagcgccga gcagttcggc gccgccacgg
     2881 tcagcgacct cacctggaag gacatcttcg acgccgacgc ctgcacgagc tgcaagcgct
     2941 gtcaggaccg ctgcccggcc tggagcaccg acaagccgct ctcccccatg aaggtcgtgc
     3001 agcagatcgg cgagctcgcc ttctccgctc ccgaggcgaa cctctgcgag acggtgacca
     3061 gcgacgtgct ctggtcctgc accacctgcc gcgcctgcca ggagatctgc ccggtgagca
     3121 tcgagcacgt caacaagatc atcgagatgc ggcgcaacct ctctctcatg gagggcgcct
     3181 tcgccggtga cgaagtgcgc accgcgatca acaacttcga ggtcaacggc aaccccttcg
     3241 gcctcgccta cgcctcccgc ggcgagtggg ccgagggcct cgacgtgcag ctcatggaag
     3301 agggtgctga cgtcgacatc ctctacttcg ccggctgcta cgcctccttc gaccagcgca
     3361 accaggtggt ggcccggagc ttcgtcaaga tctgcaacag cgccggcgtg aaggtcggca
     3421 tcctgggcaa ggaggagaag tgctgcggcg agccggcgcg caagctgggc aacgagtacc
     3481 tctaccagac gatggctcag gagaacatcg agaccatcaa ggtctacggc
//
LOCUS       DASRMJ010000164         4454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_13005, whole genome shotgun sequence.
ACCESSION   DASRMJ010000164 DASRMJ010000000
VERSION     DASRMJ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13005"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFD74_04535"
     CDS             <1..180
                     /locus_tag="VFD74_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48848.1"
                     /translation="AEVELPLPPGRWRTAFDSADDLWAGPGSALAPVLESDGTVRLSL
                     GALSAALLVPLEERV"
     gene            181..2655
                     /locus_tag="VFD74_04540"
     CDS             181..2655
                     /locus_tag="VFD74_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HZK48849.1"
                     /translation="MKRYVCIHGHFYQPPRENAWLETVEMQDSAYPDHDWNERVTAEC
                     YAPNGASRILDDHGHIRAIVNNYASMSFNFGPTLLAWLEEQAPQTYLAVLDADRQSRE
                     RFGRGSALAQTYSHMIMPLASSRDKQTQVAWGIRDFEHRFGRTPEGMWLPETAVDLET
                     LDVMAEQGLTFTVLAPRQAARIRPLDGDEDSWEDVSGSRVDPSRAYLQMLPSGRSIAL
                     FFYDGPISQAIAFEGLLRRGENFRDRLLGGFDDRREWDQLVHIATDGETYGHHHRHGE
                     MALSFALEQIDQDPDVELIDYAAYLERHPPEMEVDIVENTSWSCVHGVERWRSDCGCD
                     SGGHPDWNQAWRGPLREALDRLRGRLDPLYESAAAELLNDPWRARDDYIGIILDRSDS
                     SVAEFFSRHARRPLSPADEQQALRLLELQRHAMLMYTSCGWFFDDLSGIETVQVIQYA
                     GRALQLAGEALTNPDLERGDLQEEFERGDLEEEFLGLLAQAHSNLPQHGDGRAIFEKW
                     VRPAALDLLKVGAHYAISSLFGEYPELAPLFAYRVERRDHRLIQVGDARLAVGRIEVA
                     SRVTHTLENLSFSVLHFGGTSISCGVRLFQGEEEYQALVDDTSEAFRQMDLPQALRLL
                     DRHFGELTYSLRSLFRDEQRRILSLLMESAQNEAASLYRQIHERHAPMMLLFSALSLP
                     VPTAFRAASDFVLNVDLKTALEQPVPDPAAVRALLDEAEHDQADLDEAGLGFTLGGTL
                     ERMSADLTARPEDPDALETLLRTARLAGEMPFEVRLWSAQNHFFRVLQETYPAVAQQA
                     AAGDEEALSRVERFRALGKALWVAVD"
     gene            2727..>4454
                     /locus_tag="VFD74_04545"
     CDS             2727..>4454
                     /locus_tag="VFD74_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZK48850.1"
                     /translation="MDATYRLQLHAGFTLVDALRLLPYLENLGISHLYLSPIFAARLG
                     SVHGYDVVDPTRVNPELGGADAFEALADGLQARRMGLILDLVPNHMAADHHNPWWMDV
                     LTHGPASAHARFFDIDWASPQAPTGRVLLPFLGAPLHEVLSRGEIRPVLERPPAPEPP
                     WGEPPIGHRGSAGGPGQAGGPAHADSIREPPPELAFYARYHDRLFPLEPGTWPDVLRA
                     ADGPLPPSLETLVRDLEAIPGFRTTDERAAVLRRRTAAGARRTLAHLAATDPAAGEAL
                     RRALAALIVRPGAAPGAADAFAGILARQPWLLLHHGEARRRLNYRRFFDITDLIGVRV
                     EDEAVFAITHSLVLDMAERGRIAGVRVDHVDGLLDPTGYLTRLRDQLAERGGRHVPIW
                     VEKILSPGEQLPAEWPVAGTTGYDFLNALGHVFIDAEGLTRLGRFYDDFTGRDEPFSE
                     VRHRCKRLVLTELLGAEMRGLGERLARLSRNDPAARDEGAARGDPAGELLSPAALTEA
                     LLQVTASLTVYRTYIRDAAVCERDRVLIDEAVDDAQRRDPGRLTAALEYLRRLLSLRP
                     SLDGPSLDEA"
BASE COUNT          706 a         1631 c         1403 g          714 t
ORIGIN      
        1 gccgaagtgg agctccccct gcctccgggc cgatggcgga cggccttcga ctcggccgac
       61 gatctctggg ccgggccggg aagcgccctg gccccggtct tggagtccga cgggaccgtg
      121 cgcctctcgt tgggcgccct ctcggccgca ctgctcgtcc ccctggaaga gagggtgtag
      181 atgaagcgct acgtgtgcat ccacggccat ttctaccaac cgccgcgcga gaacgcctgg
      241 ctggagacgg tcgagatgca agactcggcc taccccgacc acgactggaa cgagcgcgtc
      301 acagccgagt gttacgctcc gaacggcgcc tcgcgcatcc tcgacgacca cggccacatc
      361 cgcgccatag tcaacaacta cgcctcgatg agcttcaact tcggcccgac ccttctagcg
      421 tggcttgaag agcaggctcc gcagacctac ctcgccgtgc tggacgccga ccgccagagc
      481 cgggagcgat tcggccgcgg ctcggccctc gcgcagacct acagccacat gatcatgcct
      541 ctggcctctt cccgcgacaa gcaaacacag gtggcgtggg gcatccgcga cttcgaacac
      601 cgcttcggcc ggacccccga ggggatgtgg ctgccggaga ccgccgtcga cctcgagacc
      661 ctcgacgtca tggccgagca ggggctcacg ttcaccgtcc tggctcccag acaggccgca
      721 cggatacgac ctctcgatgg agatgaggat tcctgggagg atgtgagcgg ttcccgggtc
      781 gaccccagtc gagcctacct tcagatgttg ccctccggcc ggagcatcgc cctcttcttc
      841 tacgacggcc ccatctcgca ggcgatcgcg ttcgagggac tcctccgccg aggggagaac
      901 ttcagggacc gcctgctggg cggctttgac gaccggcggg agtgggatca gttggtccat
      961 atcgccaccg acggcgagac gtacggccac caccacaggc acggtgagat ggcgctctcc
     1021 ttcgccctcg agcagatcga ccaggatccc gacgtcgagc tgatcgacta cgcggcctac
     1081 ctggaacgcc acccgcccga gatggaagtg gacatcgtcg agaacacctc ctggagctgc
     1141 gtccacggtg tggagcgctg gcgatcggac tgtggctgcg actccggcgg tcatccggac
     1201 tggaaccagg cttggcgcgg tcccctgcgc gaagccctcg accggctgcg cggccggctg
     1261 gacccgctct acgagtctgc ggcggcggag cttctgaacg atccttggcg tgcgcgcgac
     1321 gattacatcg gcatcatact cgaccgctcc gactcctcgg tcgcggagtt cttctcccgc
     1381 cacgcgcgcc gaccgctctc ccccgcggac gagcagcagg cccttcggct cctcgagctg
     1441 cagcgccacg ccatgctcat gtacacgagt tgcggctggt tcttcgacga tctctcaggc
     1501 atcgagaccg tgcaggtgat ccaatacgcg ggccgcgccc ttcagctggc gggcgaggcg
     1561 ctcaccaacc ccgatctcga gaggggtgac ctccaggaag agttcgagag gggtgacctc
     1621 gaggaagagt tcctgggtct cttggcccag gcccacagca acctgccgca gcacggggac
     1681 ggcagggcca tctttgagaa atgggtgcgc cccgccgccc tcgatctctt gaaggtgggc
     1741 gcgcactacg ccatcagctc cctcttcggg gagtaccccg agcttgcccc gctcttcgcc
     1801 tacagggtgg agcgccggga ccaccggctg atccaggtcg gcgacgcccg gctggcggtg
     1861 ggccgcatag aggtggcgtc ccgggtgacc cacacgctcg agaatctaag cttctcggtg
     1921 ctccacttcg gcggcaccag catcagctgc ggggtgcgtc tcttccaggg agaagaagag
     1981 taccaggctc tggtagacga cacctccgag gcgttccgcc agatggacct cccccaggcg
     2041 ctgcgtctcc tggaccggca cttcggcgaa ctcacctact ccctgcgttc gctcttcaga
     2101 gacgagcaac gccgcatcct ctccctcctc atggagtccg cccagaacga ggccgcctcc
     2161 ctctaccgcc agatccacga acgccatgcg cccatgatgc tcctgttctc cgccctctct
     2221 ctgccggtgc ccacagcatt ccgcgcggcc tccgacttcg tgctcaacgt cgatctgaag
     2281 acggccttgg agcagcccgt ccccgacccg gccgcagtgc gcgccctgct cgacgaagcc
     2341 gagcacgacc aagccgacct cgacgaagcc gggttgggct tcacccttgg cgggaccctg
     2401 gagcgcatgt ctgcggacct gaccgcccgg ccggaggatc ccgacgccct tgagacgctg
     2461 cttcggacgg cccggctggc aggggagatg cccttcgagg tgcggctctg gagcgcccag
     2521 aaccacttct tccgggtgct acaggagacc taccccgcag tggcccaaca ggctgcagcg
     2581 ggtgatgagg aggcccttag ccgggtcgaa cgtttccgtg cgctgggaaa agccctatgg
     2641 gtggccgtgg attgaagccg cctgctcggc cccggtctcc cttggggccg ggcccgctcc
     2701 tcacccgacc tgcggcggct ccgcgcgtgg acgccaccta ccgcctgcaa ctccacgccg
     2761 gcttcacgct cgtcgacgcc ctgcggctgc tcccatactt ggagaacctt ggcatcagtc
     2821 acctgtacct atcgcccatc ttcgcggccc gcctcggaag cgtccacgga tacgatgtcg
     2881 tcgaccctac gcgagtgaac cccgagctcg gcggcgcaga cgccttcgag gccctggccg
     2941 acggactgca ggcccgaaga atgggcctga tcctggactt ggtgcccaat cacatggccg
     3001 ccgaccacca caacccgtgg tggatggacg tgctcaccca cggtccggct tctgcccacg
     3061 cacgcttctt cgacatcgac tgggcttcac ctcaagctcc caccgggcgt gtgctcctgc
     3121 ccttcctggg cgctcccctc catgaggtcc tgtcgcgcgg agagatacga cccgtgctgg
     3181 aacgaccgcc ggccccagaa cctccatggg gagaaccacc catcgggcac agaggcagcg
     3241 cgggcggccc aggccaggcg ggcggcccag cccacgccga ctccatccgc gagccgccgc
     3301 ccgagttggc cttctacgcg cgctaccacg accggctctt ccccctggag ccgggcacct
     3361 ggcccgacgt cttgcgggca gccgacgggc cgttgcctcc cagcctggag acgctggtcc
     3421 gcgaccttga ggcgatcccc ggttttcgaa cgaccgacga gagggccgcg gtgctccggc
     3481 ggcggaccgc cgccggagcc cgtcgcaccc tggcccacct ggcggccacc gaccccgccg
     3541 ccggcgaagc cctgcggcgg gccctggccg cgctgatcgt ccgcccgggg gcggccccag
     3601 gtgcagccga cgcgttcgcc gggatcctgg cgcgtcagcc ctggctcctg ctccaccatg
     3661 gagaagcccg caggcgcctg aactaccgcc gcttcttcga catcaccgac ttgataggcg
     3721 tgcgtgtgga agacgaggcc gtcttcgcca tcacgcactc cctggtgctg gacatggccg
     3781 aacgggggcg catcgcgggc gtgcgcgtgg accacgtcga tggcttgctc gaccccacgg
     3841 gatacctcac caggctgcgc gatcaactcg ctgagagggg cggccgccac gtgccgatct
     3901 gggtggagaa gatcctctct cccggcgaac aactcccggc cgagtggccg gtggcgggca
     3961 ccacgggcta cgacttcttg aacgcgctcg gccacgtctt catcgatgcc gaaggtctga
     4021 cgcgattggg acgcttctac gacgacttca ccggcaggga cgaacccttc tccgaagtgc
     4081 gtcaccgatg caagcgcctt gtgttgaccg aactgctggg agccgagatg cggggtctgg
     4141 gtgagcggct cgcccggttg tcccggaacg atcccgcggc ccgagacgaa ggcgcagctc
     4201 ggggcgatcc cgcgggcgaa ctcctttcgc ccgccgccct gaccgaggcc ctgctccagg
     4261 tgacggcctc actgaccgtc tatcgcacct acatcagaga tgccgccgtc tgcgagcgcg
     4321 accgcgttct catagacgaa gccgtggacg acgcgcagag gcgcgacccc gggcgcctga
     4381 cggcggccct cgagtatctg cgccgtctac tcagccttcg cccgtcgctt gatggaccgt
     4441 ctcttgacga agcc
//
LOCUS       DASRMJ010000165         5092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_130445, whole genome shotgun sequence.
ACCESSION   DASRMJ010000165 DASRMJ010000000
VERSION     DASRMJ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130445"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..1056)
                     /locus_tag="VFD74_04550"
     CDS             complement(289..1056)
                     /locus_tag="VFD74_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZK48851.1"
                     /translation="MRLARVLFEGRPETVRVFEGSYEVVSGERKGLAFASQGLRILPP
                     AVPSKVIGVGWNFPAHIDEMVARLPKDAPSSVPEEPIIFLKPPSSLIGHGDAIVYPRE
                     ATKVEHEGELCLVIGRALRRATADEARDAVLGWTCGNDVTERDMQRRDRQWWRAKGFD
                     TFAAVGPYLDTEPPDPDARIRTLVNGTVRQEGRVCDMIRDPFTIVSFVSQAMTLLPGD
                     LLMLGTPPGVGELHPGDLVVVDIDGLEPLRNPVVAEG"
     gene            complement(1405..3240)
                     /gene="feoB"
                     /locus_tag="VFD74_04555"
     CDS             complement(1405..3240)
                     /gene="feoB"
                     /locus_tag="VFD74_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZK48852.1"
                     /translation="MVQAPEGARKIVLAGNPNVGKSLFFNAFTGLYVDVSNFPGTTVA
                     VSSGVMGDDVLLDTPGIYGVSSFNDEETVARDIITEADVVLNVVDAVHLERDLFLTVQ
                     LIDMGLPVVVALNMMDEAESRGMSIDAAALAAWLGVEVVPTIAVSRKGFDRVEQALAH
                     ACPGTSDPVLQDKLLGLLDRVGTKAEALLVMEGDPFVAERHGIEPMGLRDEVYLDRRR
                     RVNDIVAKVVQQRDRSLDLRTRLGHALMRPLTGLPVLAVVLWLMYEVIGVLVAQTIVG
                     FTEGTIMRGQVEPALRSVIGSVAGSGVIYQLLAGEYGVVTMTITYIFGLLLPLVIGFY
                     LLLSVLEDSGYLPRIAALTDRMMTAIGLNGRAIIPIILGFGCITMATITTRILGNERE
                     RKIATALMAFAIPCSAQLGVIVALLAAAGGVGITLAYAAIMFVVFGLIGLGLAHYLPG
                     SSTDLLIDLPPLRLPRPLNVIQKTYHKTVMFLREVSLYFALGALLIGVLDLTGALVAA
                     QGVMAPLTENWLHLPREAATAFIMGFVRRDFGAAGLYSLGLGGGATLVALVTITLFVP
                     CIASVLVIMKERGRTFTAFTWVGSVVLAFAVGGVIAQTLGWLGVA"
     gene            complement(3282..3599)
                     /locus_tag="VFD74_04560"
     CDS             complement(3282..3599)
                     /locus_tag="VFD74_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HZK48853.1"
                     /translation="MTLLQAKKGRSVRIIEIPDDRARAQCVRFGIGEGTIVDSTETLM
                     AGPVLVRHRNQEICVGRKLASSILVEIDPEGASGRGRSEDEASGVARGHRLRWRAHHS
                     RSV"
     gene            complement(3829..>5092)
                     /gene="cimA"
                     /locus_tag="VFD74_04565"
     CDS             complement(3829..>5092)
                     /gene="cimA"
                     /locus_tag="VFD74_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HZK48854.1"
                     /translation="TWALHIEKVLKVDRDENLRMIDESIRFLRGEGKEVVYDAEHFFD
                     AYAEHPEYALECLRAAAAAGAGTVVLCDTNGATLPAVLGEVVEAVNASIGDLADLGIH
                     THNDTSCAVANSLVAVEKGVVHVQGTINGYGERCGNADLCAIIPSLKLKMGRDCITDA
                     NLARLTDTAHFVAELCNVSPDPQQAYVGRNAFAHKGGLHVSGIAADPRTFEHVPPAAV
                     GNVPHILVSELSGKGTVRRRLEDLGIQADSGDEVVERLLQRLKQKEHDGYHYEAADAS
                     FELLLLAELGRGVEFFRLESFRIIVEKRADGAVMTEATIKVHVDGERVIQTAEGNGPV
                     NALDKALRLAIQRKFPQLGDIHLVNFKVRILDEAKGSGAVTRVLIDSSDGQDSWGSVG
                     VSENLVEASWQALVDSVNYGLRRPRAVD"
BASE COUNT          902 a         1737 c         1656 g          797 t
ORIGIN      
        1 tgccagggca gagaatgcga cgcggccgga tcgtgccgag cctccgacgc gccccacgat
       61 tctacccgaa acccccccgc ggcctcaagc accaggttgc ctaccagcgg tatttcgtcc
      121 gctcacagca acacgccggt acgggcggat aggcggtccg tgggcccctg agcgggagcg
      181 gtagagggcg ggcacgaggt ccttgcggac ccgtgaacgg tcgaaagcgg cgggtggccg
      241 aaacctcggg cgagggcgcc gcagtggccg gcgcagttgg cggccgccct acccctccgc
      301 gacgaccggg ttccgcaggg gctccagccc gtcgatatcc acgaccacca agtcgccggg
      361 gtggagctcg cccactccgg gcggcgtccc cagcatgagg agatcacccg gaagaagcgt
      421 catggcctgg ctgacgaagg agacgatcgt gaagggatcg cggatcatgt cgcacaccct
      481 cccctcttgg cgaacggtgc cgttgaccaa cgtgcggatc ctcgcgtccg gatcaggcgg
      541 ttccgtgtcc aagtaggggc ccacggcggc gaacgtgtcg aaccctttcg ccctccacca
      601 ctggcggtct cttctctgca tgtcccgttc ggtcacgtcg ttgccgcagg tccaccccaa
      661 cacggcgtcc cgggcctcgt cggctgtagc ccgacgcagc gcccgcccga tcaccaggca
      721 cagttctccc tcatgctcca ccttagtcgc ctcgcgcggg tacacgatgg cgtcgccatg
      781 gccgatgagg ctcgacggag gcttcaggaa gatgatgggc tcctcgggaa cgctgctggg
      841 cgcgtccttg ggaagccgcg ccaccatctc gtcgatgtgc gcggggaagt tccaccccac
      901 gccgatcacc ttactcggga cggcgggcgg aaggatcctc aggccctgcg acgcgaacgc
      961 caaacccttg cgttcgcccg acacgacctc atacgacccc tcgaacacac ggaccgtctc
     1021 cggacggccc tcgaacagga cacgagcaag tctcacgcca ccctcatttc actctcagcc
     1081 cgaccggctt ggatcagcgc gcacctgcgc tccgtcacat ttgaccagtg tcgcggagcc
     1141 gtgtcaaacg tggcaggcgt agggcgggcg gccctgcggg ttcgggctcg ttcggtcggc
     1201 gcctttcgag tggcacgacg agcagtcccc gtcgcagcca agtgagagcg gctctcgaca
     1261 gcgcgggcac atcagcgcga ccgctcccat ccggagccca caccgcgggc acgtgacgaa
     1321 cacttggcgc gagtcgtcac tcatctcgac gcgccgcccc cgggcggcac cgtctggagc
     1381 agccgcctgt ccaggtccgc agtctcacgc cacccccagc caaccgaggg tctgtgcgat
     1441 gacgccgccg acggcaaagg ccagcaccac actgcccacc caggtgaacg cggtgaaggt
     1501 ccggccccgc tccttcatga tcaccaggac cgaggctata cacggcacga agagcgtgat
     1561 ggtgacgagg gcgacgaggg tagctcctcc accgaggccc agggagtaga gaccggccgc
     1621 gccgaagtcc ctgcgcacga accccatgat gaacgccgtg gcggcctcgc ggggaaggtg
     1681 cagccagttc tcggtgagcg gcgccatcac gccttgcgcg gccacgagcg ccccggtgag
     1741 gtcgaggacc ccgatgagga gcgctccgag cgcgaagtag agcgatacct cacgcaagaa
     1801 catcaccgtc ttgtgatagg tcttctggat gacgttcagc ggccgtggca ggcgcagcgg
     1861 cggcaggtcg atgagaaggt ccgtcgagct gccgggcaga tagtgagcga gcccaaggcc
     1921 gatcaacccg aacacgacga acatgatggc cgcgtaggca agcgtgatac cgaccccgcc
     1981 ggctgcagcc agcagagcga cgatcacacc gagttgggcc gagcagggga tggcgaaggc
     2041 catgagagcg gtcgcgatct tgcgctcccg ttcgttgccc aggatgcggg tggtgatggt
     2101 ggccatggtt atgcagccga agccgaggat gatcgggatg atcgcgcgtc cgttgaggcc
     2161 gatggcggtc atcatccgat cggtgagcgc ggcgatccgc ggcaggtagc cggagtcctc
     2221 gagcaccgag aggaggaggt agaaccctat gacaagcgga aggaggagac cgaatatgta
     2281 ggtgatggtc atggtgacca ccccgtactc gcccgcgagc aactgataga tcacgccgct
     2341 ccccgcgacg ctcccgatga cggaccgcag ggcgggctcg acctgacccc gcatgatcgt
     2401 gccttcggtg aacccgacta tggtctgcgc gaccagcact ccgatgacct cgtacatcag
     2461 ccagagcacc accgccagca ccggcagacc ggtgagcggc cgcatcaggg cgtggcccag
     2521 gcgggtgcgc aggtcgagac tgcggtcgcg ctgctgcacg accttggcaa cgatgtcgtt
     2581 cacccggcgg cgtcggtcga ggtagacctc gtcacgcagg cccatgggct cgataccgtg
     2641 ccgttcggcc acgaaggggt cgccctccat caccagcagg gcctccgcct tcgtgcccac
     2701 gcggtcgagg agaccgagca gcttgtcttg cagcaccggg tcggacgtgc ccgggcacgc
     2761 gtgggccaga gcctgctcca ccctgtcgaa ccccttccgc gacacggcga tcgtaggcac
     2821 gacctcgacg cccaaccaag cggccaaggc ggcggcgtcg atactcattc cccgactctc
     2881 cgcctcgtcc atcatgttga gagcgaccac gacaggaagg cccatgtcga tgagttgaac
     2941 ggtaaggaac aggtcgcgct cgaggtgcac cgcgtccacc acgttgagca ccacgtcggc
     3001 ctcggtgatg atgtcgcgcg ctaccgtctc ctcgtcgttg aaggaagaga cgccgtagat
     3061 gccgggagtg tcgagcagta cgtcatcgcc catcaccccg ctggacaccg ccaccgtcgt
     3121 gccggggaag ttggacacgt ccacgtaaag cccggtgaag gcgttgaaga agagcgactt
     3181 gcccacattg gggttgccgg ccaggacgat cttgcgggca ccctcggggg cctgcaccac
     3241 cggtgctccg tggcaggcgt ggcgcgaagc cgagtccacg gtcagaccga gcggctgtga
     3301 tgcgcgcgcc accgaaggcg gtggccgcga gcgactccgg acgcctcgtc ctcgctccgc
     3361 ccgcgccccg aggctccttc agggtcgatc tccaccagga tgctcgaagc cagcttgcgg
     3421 cccacacaga tctcctgatt gcgatgccgc acgaggaccg gaccggccat caaggtctcc
     3481 gtagaatcaa cgatcgtccc ctcgccgatg ccgaaacgca cgcactgagc gcgggcgcga
     3541 tcgtcgggga tctcgatgat ccgcaccgaa cggcctttct tggcctgaag aagcgtcatg
     3601 tcccgtgcct ccgatcgttg agaggagggt tggctgcact ttcatattag gtatccctca
     3661 ctcgctgagc aagtgtactc ttccccggcc gcgccgtcaa gcgaccgggt ggagggcaag
     3721 aggagtctct acacgtgtga ggtgggcagc cggtgtcggc cgccggtgcc ggctggagcg
     3781 tgacgccgac gcgccgcggg aaggcctacc ggcggcgaga gcgaagagtc agtcgaccgc
     3841 ccgagggcgg cgcagaccgt agttcacgga atcgaccaga gcctgccagg aagcctccac
     3901 caagttctcg ctcaccccga cgctgcccca cgaatcctgt ccgtcggatg agtcgatgag
     3961 cacgcgggtc acggcgcccg aacccttcgc ctcgtccaag atccgcacct tgaagttgac
     4021 gaggtggata tcgccgagtt gagggaactt gcgctgtatc gccagtcgca gcgccttgtc
     4081 gagcgcgttc accgggccgt tcccttcggc ggtctggatc acgcgctcgc cgtcgacgtg
     4141 caccttgatg gtcgcttccg tcatgacggc gccgtcggcg cgcttctcca cgatgatgcg
     4201 gaagctctcc agacgaaaga attctacgcc gcgccccagt tcggccagca gcagcagctc
     4261 gaacgacgcg tcggccgctt cgtagtggta gccgtcgtgt tccttctgct taaggcgctg
     4321 cagcaggcgt tccaccacct cgtcgcccga atcagcctgg atgccgaggt cctccagacg
     4381 acgacgcacc gtgcccttgc ccgacaactc cgagaccagg atgtggggca cgttgcccac
     4441 cgccgcaggc ggcacatgct caaaggtgcg tgggtcggcc gcgatgcccg agacgtgcag
     4501 gccgcccttg tgcgcgaacg cgttccggcc cacataggcc tgctgcggat cgggcgacac
     4561 gttgcagagt tccgcgacga agtgggcggt gtcggtgagt ctggcaaggt tggcgtcggt
     4621 gatgcagtcg cgccccatct tgagcttgag cgaggggatg atggcacaga gatcggcgtt
     4681 gccgcaccgc tcgccgtagc cgttgatcgt cccctggacg tggaccacgc ccttctccac
     4741 cgccaccagc gagttcgcga cggcgcacga ggtgtcgttg tgcgtgtgga tgcccagatc
     4801 ggcgagatcc ccaatagacg cgttgacggc ctcgacgacc tcacccaaga ccgccggcag
     4861 cgtggctcca ttcgtgtcac acaggaccac cgtcccggct ccggccgcgg ccgccgcgcg
     4921 caggcactcg agggcgtact cggggtgctc cgcataggcg tcgaagaagt gctctgcgtc
     4981 gtagaccact tccttgcctt cgcccctcaa gaagcggatg gactcgtcga tcatccgcag
     5041 attctcgtcg cgatcgacct tcagcacctt ctcgatatgg agagcccagg tc
//
LOCUS       DASRMJ010000166         7920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_48892, whole genome shotgun sequence.
ACCESSION   DASRMJ010000166 DASRMJ010000000
VERSION     DASRMJ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48892"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFD74_04570"
     CDS             complement(<1..436)
                     /locus_tag="VFD74_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48855.1"
                     /translation="MRSSRTRWALAGALVLILPFVAGCKIEMALDTSIDAGGSGTIGV
                     RLAADKEIQDLIASQGGAQGDLFGEFEGSVPEGWASDSGTDADGTSWVTASRAFSDPS
                     EITAFLEEGQDGPAASIGAQAFSLTQDKGLLSVRTVFSGTWDM"
     gene            complement(433..729)
                     /locus_tag="VFD74_04575"
     CDS             complement(433..729)
                     /locus_tag="VFD74_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48856.1"
                     /translation="MLHLCLHPPQRCAHDDGFDDGVVVMSVWAMQVGAVVLGLCALAL
                     FAFAGAYYLRNRRIALAARERHPEAAVGVKGAGVLRPRTERPEKEGRQEREGSR"
     gene            1095..2270
                     /locus_tag="VFD74_04580"
     CDS             1095..2270
                     /locus_tag="VFD74_04580"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZK48857.1"
                     /translation="MSGFLVLDVSRVLAGPYATMVLADLGAEVIKIERPGAGDDSRSF
                     GPFLHGSSGYFASVNRGKKSVTLDLASEGGRDTFIALARQADILVENFRPEVMDRLRL
                     GWDDLREINPRLIYASCSGFGQTGDYARRPAYDIVVQAMGGLLGITGEEGGGPVRVGV
                     SLGDLSAALFLVVGILAAALQRESTGRGQTVDVAMLDSVVALLENAIVRNDLESKTPG
                     PLGTRHPSIAPFQAFAAQDGYMVVGAGNDRLWQRLCAVLQEDGLAADPRFATNAGRND
                     HHAELEERLSRVFITRPKTEWLMMLEQAGIPCGPVNGVDDVVVDPQVRSRDMLVSVTD
                     SEGNTFRVASSPVRLSEQGIRPRGWVAGLGEHTVEVLTQRLGLDGETVEALRRQAAV"
     gene            2290..3672
                     /gene="hydA"
                     /locus_tag="VFD74_04585"
     CDS             2290..3672
                     /gene="hydA"
                     /locus_tag="VFD74_04585"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HZK48858.1"
                     /translation="MRSIVTNGIVVNAGGEYRADVLIENGTIVAVGTGLPADGATVID
                     AAGAYVLPGGIDVHTHLDMPFGGTTSADDFETGTKAAAVGGTTAIVDFAIQAKGQSLG
                     DALATWKAKAEGKAVIDYGFHVAVTDLNDATLAEIPGLVEAGVTSLKLFMAYKGALMV
                     DDAALLRVLKKGKEAGALISVHAENGDVIDVMVKEHLAAGLTAPKYHAATRPPEAEGE
                     ATGRAIFLAGLADAPIYIVHLSCKEALDKVRAARDAGLPVYAETCPQYLALSVDNYEE
                     ADFEGAKYVCSPPLRDKSNWNELWAGITAGDLQVIATDHCPFNFKGQKDLGRDDFSKI
                     PNGAPGIEHRLVLLNTLGVQTGRINMAKLVELTSAAPARFFGMFPEKGAIAPGSDADL
                     VIFDSAAVSVITAASQTQNLDYTPYEGMAVKGAVRAVMQRGTMVVEDGTFVGAAGAGK
                     YLKRKPFSLI"
     gene            3696..4877
                     /gene="preA"
                     /locus_tag="VFD74_04590"
     CDS             3696..4877
                     /gene="preA"
                     /locus_tag="VFD74_04590"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HZK48859.1"
                     /translation="MTDLLRTDFTGLSFPNPFILASAPPTRTGDMIKRAFEAGWGGAA
                     TKTIGLQGAVNVRPRFGFLKERSRIVGMTNFELISEKGLDHWVDEIADIKAAFPDRPL
                     IASIMGSVEPTDWEILAKAVQAAGADAIELNVSCPHGMPEQHMGAFMGQDPALIKMAT
                     AAVAGASSVPVWVKLTPNVTDIAAMSLAAKEAGADAVAAINTVAGLVGIDIETFRPVP
                     SVAGRSAYGGYSGQGVKPVALRAVSSIAATSKLPVSGCGGITTWEDAAEFMLAGAGTL
                     QVCTAVMLNGYGIIDVFVRGLSEYLERKGFDSYASAVGRSLPMIGEFSALDPHVGAHA
                     VVDPEACNGCGNCVPACGDGGFQAIVMCGKVAVVDSELCDGCSLCESVCRVSAVSMVR
                     G"
     gene            4979..6448
                     /locus_tag="VFD74_04595"
     CDS             4979..6448
                     /locus_tag="VFD74_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HZK48860.1"
                     /translation="MSENSLDLVKTKTGLYELTADVSSSSLYNKDIGPTKVAQRTWST
                     YNIAALWIGMSVCIPTYMLAAGLVAGGMSAWQAVFTVMLGNVIVLIPMVLNAVPGTKF
                     GIPFPVVVRASFGTLGSNIPALLRAGVACGWFGIQTWIGGSALNAMMLALWSGWPEAG
                     AGKWIAFLVFWAINVWIIVMGMNFLKKFESWAAPILIAMGILMLIWAMSKAGGIGPIM
                     SEPSKFATFGEFWKFFIPGLTGMVGFWATLSLNIPDFSRFARDQKAQIYGQVLGLPTT
                     MTLYAFLGVAITSATVIIFGEALWDPVAVLQKFDNPLIIVVGMFWLAVATLSTNIAAN
                     VVSPANDFQNLWPRKINFVIGGLITAVIGIAIRPWWLLSDYGNYIFNWLGTYSGLLGP
                     VAGIMIMDYFIIRKGQLNVRDLYLRGGEYEYTGGFNFKAIGAFVIAAGATLIGKWVPS
                     VHWLADYSWFVGFALGFVIYYALMFNTKVTSLEGVPEAK"
     gene            complement(6594..7817)
                     /locus_tag="VFD74_04600"
     CDS             complement(6594..7817)
                     /locus_tag="VFD74_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK48861.1"
                     /translation="MRAGRFMNRRTAVFGLFTAAYFLSYFFRSANAVIAPDLSRDLHL
                     GARELGLMTSLFYAAFAAVQVPLGAALDRFGPRVVTPGFMLVTAVGAVVFATSHSFAQ
                     VALGRALIGMGMAGVFMGSLKVFSQWFSLRRFARVSGYLVGLGAMGALAAGTPLALVV
                     QAMGWRSVFALGAALVVLSSLSIFLWTRNAPVGVEWRSNDETRGSVLQVFRDARFWRI
                     AALDFFLVGTLLSMQGLWGGPFLYDVFGLSQVRTGNLLILLSLGAIAGYVSCGSFAER
                     FGTPRTLTWTSSAFVAAQVVMISAALLGHLWMVWIAYPVFGFAGAFNVLLMAHSRSVF
                     PTSMTGRAVTALNLFGIGGSAVIQWGMGMVIGSFGRDSADHYPPMAYAVALGITAVGG
                     ALSLLWYRSLAHRSG"
BASE COUNT         1451 a         2627 c         2541 g         1301 t
ORIGIN      
        1 gcatgtccca cgtgccggag aacacagtcc tcaccgagag gagtccctta tcctgcgtga
       61 gcgagaacgc ttgggccccg atactcgcgg cgggaccatc ttgaccttct tccaggaagg
      121 cagtgatctc ggagggatcg ctgaacgccc gcgaagcagt gacccaactg gtgccgtcgg
      181 cgtcggtgcc agagtctgaa gcccagcctt ccggcacgct gccttcgaac tctccgaaga
      241 gatcaccctg cgctccccct tgggaggcga tcaggtcctg gatctccttg tcggcggcga
      301 ggcggacgcc gatcgttccg gatccgccgg catcgatgct cgtatccagt gccatctcga
      361 tcttgcagcc cgccacgaac ggcaggatca gaactagcgc cccagccagg gcccaccttg
      421 tccgcgaact cctcatcggg atccctccct ctcttgccgc ccttctttct cgggccgttc
      481 ggtccgtggc cgcaggacgc ccgcgccttt cactccgacg gcggcctccg ggtgacgttc
      541 gcgcgccgcg agcgcaatcc tgcgattgcg gaggtagtag gcgccggcga acgcgaagag
      601 cgcaagggca cacagcccca ggacgacggc gcccacctgc atggcccaga cggacatcac
      661 gaccacgccg tcgtcgaagc cgtcatcatg tgcacagcgc tgcggcggat gcagacataa
      721 gtgcagcata ccactggact ccaaggagaa gccgccgcta atgtggactg attcgaggcg
      781 cgggtagatc aaagcgtcgg cggatctccc ggggggatgt caacgagcag gaccgcatcg
      841 tcgcgcgcag ggtcttcacg tgcgttgact tgacatccgg tcaaacagta ttctgaccct
      901 catcgaagag agcaccgcag agcctgcgga gatgccctgt tctatatcga tccgatcgcc
      961 gatcgcagag tcacgggggc ggcgccattc ccgagtttga agacacccac cgggcggatg
     1021 tggccggagc agacgactcc tcgacagccg cgaccccctc gcggcacgcc gacgccaccc
     1081 agccgccggg ccccttgtca ggcttcctcg tcctcgacgt gtcacgggtg ctcgccggac
     1141 cctacgcgac gatggtgctc gccgacctgg gcgccgaggt catcaagatc gagaggccgg
     1201 gggccgggga cgactcgcgc tcgttcggcc ccttcctgca cggttcgagc ggctacttcg
     1261 cgagcgtcaa ccggggcaag aagagcgtca cactcgacct ggcatccgag ggcggccgcg
     1321 acaccttcat cgcgctcgcg cggcaagccg acatcctcgt ggagaacttc cgccccgaag
     1381 tcatggacag gctccgcctc ggttgggacg acctcaggga gatcaaccct cgcctcatct
     1441 acgcttcgtg ttcgggtttc ggtcagacag gcgactacgc gcggcgcccc gcctacgata
     1501 tcgtcgtaca ggccatgggc ggccttctcg gcatcaccgg tgaggagggc ggagggccgg
     1561 tcagagtggg cgtgtctttg ggggatctgt cggcggctct cttccttgtg gtgggcatcc
     1621 ttgccgcggc gctccagcgg gagagcaccg gcagaggaca gacagtcgac gtggcgatgc
     1681 tggactctgt ggtggcgctc ctcgagaatg cgatcgtccg gaacgacctg gaatcgaaga
     1741 ctcccggccc gctcgggacg cggcaccctt cgatcgcccc gttccaggcc ttcgcggccc
     1801 aagacggcta catggtggtg ggcgccggga acgaccggct atggcagcgg ctgtgcgctg
     1861 tgctccagga ggacggcctc gcggctgacc cacgcttcgc cacgaacgcg gggcgcaacg
     1921 accaccacgc cgaactcgag gagagactct cccgcgtctt catcacgcgg ccgaagacgg
     1981 agtggctcat gatgttggag caggccggga tcccgtgcgg gcccgtcaac ggagtcgatg
     2041 acgtcgtggt cgacccgcag gtgcgctcgc gcgatatgct tgtctccgtg accgacagcg
     2101 aggggaacac gttccgcgtc gcgtcaagcc ccgtgcgttt gtccgagcag gggattcggc
     2161 ctcgcggatg ggtggccggt ctcggcgagc acacggtcga ggtgctgaca cagcggctcg
     2221 gcctggacgg agaaacggtg gaagcgctgc gccgacaagc agccgtctag cgaagaaagg
     2281 gagtgtgacg tgagaagcat cgtcaccaac ggcatcgtgg tgaacgccgg cggagagtac
     2341 cgggcggacg tactgataga gaacggcacg atcgtggctg tgggaaccgg acttcccgca
     2401 gacggcgcga cggtgataga cgcggccggc gcctacgtgc tccccggcgg gatcgatgtg
     2461 catacacacc tcgacatgcc gttcggaggc accacctcag ccgacgattt cgagaccggc
     2521 acgaaggccg ccgccgtggg cgggaccacc gctatcgtcg acttcgccat ccaggccaaa
     2581 ggccagtctc tcggcgacgc tctcgccacc tggaaggcaa aggccgaggg caaggccgtc
     2641 atcgactacg gcttccacgt ggccgtgacc gacctgaacg atgccaccct cgccgagatc
     2701 ccggggctcg tggaggccgg cgtcaccagc ctcaagctct tcatggccta caagggcgcc
     2761 ctcatggttg acgacgcggc gctcctgcgg gtgctcaaaa aggggaaaga ggccggcgcc
     2821 ctcatcagcg tccacgccga gaacggcgac gtcatcgacg tgatggtgaa ggagcacttg
     2881 gcggccggac tcaccgctcc gaagtaccac gcggccaccc ggccgccgga agcggagggt
     2941 gaagccaccg ggcgcgccat cttcctggca gggctggcgg acgctcccat ctacatcgtc
     3001 cacctgagct gcaaggaagc tctcgacaag gtcagagccg cacgcgacgc cggtctgccg
     3061 gtgtatgccg agacgtgccc tcagtacctc gcgctgtcgg tggacaacta cgaggaagcg
     3121 gacttcgagg gcgccaagta cgtctgctct cccccgcttc gcgacaagag caactggaac
     3181 gaactgtggg cggggatcac cgccggcgac ctacaggtga tcgccaccga ccactgcccc
     3241 ttcaacttca agggtcagaa ggacctgggc cgcgacgact tcagcaagat cccgaacgga
     3301 gcccctggca tcgagcaccg gctggtgctg ctcaacacac tgggcgtgca gaccgggcgc
     3361 atcaatatgg cgaagctcgt tgagctcacg tcggcggcgc cggcacgctt ctttggcatg
     3421 ttcccggaga agggtgcgat cgcccccggc agcgatgccg acctcgtgat cttcgactcc
     3481 gcggccgtca gtgtcatcac cgccgcctcc cagacgcaga acttggacta cacgccctac
     3541 gaaggcatgg ccgtcaaggg cgccgtgcgg gccgtcatgc aacgcggcac catggtcgtc
     3601 gaggacggca cgttcgtcgg agccgcgggc gccggcaagt atctgaagcg caaaccgttc
     3661 tcgctcatct gagccgggaa ccgagggaga gatacgtgac cgacctacta cgcaccgatt
     3721 tcacggggct gtcgttcccc aaccccttca tcctggcgtc ggcgccgccg acgcggaccg
     3781 gcgacatgat caagcgggcc ttcgaagccg gctggggcgg cgccgccacc aagaccatcg
     3841 gcctgcaagg cgctgtgaac gtcaggccac gcttcggatt cttgaaggag cgcagccgga
     3901 tcgtgggcat gaccaacttc gagttgatct cggagaaggg tctcgaccac tgggtggacg
     3961 agatcgccga catcaaggcc gcgttccccg atcgcccctt gatcgccagc atcatgggtt
     4021 cggtggagcc gacggattgg gagatcctgg cgaaagcagt ccaagcggcc ggagccgacg
     4081 ccatcgagct caacgtgtcc tgcccgcacg gcatgcccga gcagcacatg ggagccttca
     4141 tgggccaaga ccccgcgcta atcaaaatgg ccacggcggc cgtcgcgggg gcctcgtccg
     4201 tcccggtgtg ggtgaagctc acgccgaacg tcaccgatat cgccgcgatg tccctggccg
     4261 cgaaggaggc tggagccgac gccgtcgccg ccatcaacac agtggccgga ctggtgggca
     4321 tcgacatcga gactttcaga ccggttcctt cggtggccgg gcgcagcgcc tacggcggat
     4381 actcgggcca gggcgtgaag cccgtcgcgc tgcgcgccgt ctcgtccatc gcggccacat
     4441 cgaagctgcc cgtcagcggg tgcggcggca tcaccacctg ggaggacgcc gccgagttca
     4501 tgctggccgg cgcgggcaca ctgcaggtgt gcaccgccgt catgctcaac ggctacggca
     4561 tcatcgacgt cttcgtccgc ggtctctccg agtacctcga acgtaagggt ttcgacagct
     4621 acgcgagcgc cgtagggcgc tcgctgccca tgatcggcga gttctcggcg ctcgaccccc
     4681 acgtaggcgc ccacgccgtg gtcgatcctg aagcatgcaa cggctgcggc aactgcgtcc
     4741 cggcttgcgg cgatggtggg tttcaagcta tcgtgatgtg tgggaaggtc gccgtggtcg
     4801 attctgagct gtgcgacggt tgttctctgt gtgaatccgt gtgccgagtg tccgccgtca
     4861 gcatggtgcg agggtgagtc gtgccctcga attcagctcc ctgtgtaacc gtccgcccgc
     4921 tgctggggcc accggacgtc gacatagtca gatccaacca ctcgcctagg gggatacttt
     4981 gagcgagaac agcctcgatc ttgtcaagac aaagacagga ctctacgagc tgacggccga
     5041 tgtctcgagc agttcgctgt acaacaagga cattggcccc acgaaggtgg cacaacgtac
     5101 gtggagcacg tacaacatcg ccgccctctg gatcgggatg agcgtctgca tcccgacgta
     5161 catgttggca gccggcctgg tggccggtgg tatgagcgct tggcaggccg tgttcacggt
     5221 catgctcgga aacgtcatcg tcctgattcc catggttctg aacgccgttc ccggcaccaa
     5281 gttcgggatc cctttcccgg tcgtggtccg tgcgagcttc ggtactctgg gctctaacat
     5341 ccccgccctt ctgcgggccg gcgtcgcctg cggttggttc ggtatccaaa cctggatcgg
     5401 cggttccgct ctcaacgcca tgatgctggc gctctggagc ggatggccgg aagccggcgc
     5461 cggcaagtgg atcgccttcc tcgtgttctg ggcgatcaac gtgtggatca tcgtcatggg
     5521 catgaacttc ctcaagaagt tcgagtcctg ggccgccccg atcctcatcg cgatgggtat
     5581 cctcatgctc atctgggcca tgagcaaggc cggcggtatc ggcccgatca tgtccgaacc
     5641 gagcaagttc gccaccttcg gcgagttctg gaagttcttc atcccgggtc tgaccggcat
     5701 ggtgggtttc tgggccactc tgtcgctcaa catccccgac ttcagccggt tcgcgcgcga
     5761 ccagaaggcg cagatctacg gccaagtgct gggcctgccc accacgatga ccctctacgc
     5821 cttcctgggc gtggccatca cctcagctac cgtcatcatc ttcggcgaag cgctgtggga
     5881 tccggttgcc gtgctccaga agttcgacaa cccgctgatc atcgtggtcg gcatgttctg
     5941 gctcgcagtg gcgaccctgt ccacgaacat cgcggccaac gtggtatcac ccgcgaacga
     6001 cttccagaac ctgtggccta ggaagatcaa cttcgtcatc ggcggtctca tcaccgccgt
     6061 catcggcatc gccatccgtc cgtggtggct gctgagcgac tacggcaact acatcttcaa
     6121 ctggctcggc acctacagcg gcctcctcgg ccctgtcgcc ggcatcatga tcatggacta
     6181 cttcatcatc cgcaaaggtc agctgaacgt gcgcgacctg tatctgcgtg gtggtgaata
     6241 cgagtacacc ggcggcttca acttcaaggc catcggcgcc ttcgtcatcg ccgccggcgc
     6301 aaccctgatc ggcaagtggg tgccttcagt gcactggctc gccgactact cgtggttcgt
     6361 cggattcgcg ctcggtttcg tgatctacta cgcgctgatg ttcaacacca aggtcacaag
     6421 cctcgaaggt gttccggaag ccaagtagcc aatcacaacg cttcgagatc gaccaaaagg
     6481 acgggcggcc cgttgggccg cccgtccttc ttcttgcccc cgcgggggac cgttcgacgg
     6541 acgaggaccg gagtggaccg tctgaagcag agcggtctcg ggcgggggtc ggcctagcct
     6601 gaccggtgcg ccagggagcg gtaccacagg agactgaggg cccctcctac cgcggtgatg
     6661 cccagcgcca cggcataggc catcggggga tagtgatccg ctgagtcgcg gccgaacgaa
     6721 ccgatcacca tccccatgcc ccattggatg accgcgctcc cgccgatgcc gaacagattg
     6781 agggcggtca cggcccgccc cgtcatcgac gtgggaaaga cggaacggga atgtgccatc
     6841 aggagaacgt tgaaggcccc ggcgaagccg aagacggggt acgcgatcca caccatccac
     6901 agatgcccga ggagcgccgc cgagatcatg acgacctgag ccgccacgaa cgcactggat
     6961 gtccaggtga gcgtgcgtgg tgtgccgaag cgctcagcga acgatccgca actgacgtag
     7021 cccgcaatcg ctcctaggga gagcaggatg agcaggttgc cggtgcgcac ctgcgaaagg
     7081 ccgaagacgt cgtagaggaa gggccctccc cacaggccct gcatggagag cagagtcccg
     7141 acgaggaaga agtccagtgc ggcgatgcgc cagaaacgcg catcgcggaa cacctggagc
     7201 acgctccctc gggtctcgtc gttcgatcgc cactcgacgc ccaccggtgc gttccgggtc
     7261 cataggaaga tgctgaggga actcagcacc acgagtgcgg cacccaacgc gaagaccgac
     7321 cgccacccca tcgcctgtac caccagcgcg agcggagtcc ccgccgccaa cgcgcccatg
     7381 gccccgagcc ccactaggta tcccgacacc cgggcgaacc ttcgcaggga gaaccactga
     7441 ctgaacacct tgagggaacc catgaacacg ccggccatgc ccatcccgat caaggcgcga
     7501 ccgagcgcca cctgtgcgaa ggagtgcgag gtcgcgaaga ccaccgcgcc taccgcggtc
     7561 accagcatga accctggcgt cacgacacgc ggcccgaaac ggtcgagggc ggctccgagc
     7621 ggcacctgca ccgctgcgaa agccgcgtag aagaggctgg tcatcaaccc cagctcccga
     7681 gcgcccaagt ggaggtcccg cgacaggtcg ggggctatga cggcgtttgc agatcggaag
     7741 aaataggaga ggaagtaggc ggcggtgaag agcccgaaga ccgctgttcg ccgattcatg
     7801 aacctgcccg cgcgcactcg ccccccgttc ctacagacgg accggcacgc cagcggtggc
     7861 catccgttcc gctggcgtgc cggtccgtct gtaggaacgg ggggcgagtg cgcgcgggca
//
LOCUS       DASRMJ010000167         4807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_130559, whole genome shotgun sequence.
ACCESSION   DASRMJ010000167 DASRMJ010000000
VERSION     DASRMJ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130559"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /gene="nusA"
                     /locus_tag="VFD74_04605"
     CDS             complement(<1..818)
                     /gene="nusA"
                     /locus_tag="VFD74_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZK48862.1"
                     /translation="MNRETIDALRQIEKEKSIPFGILIEALEEALRSAYKKTANAVPF
                     SDVEIDQDTGEIHVFQLTFPPGLEPDLDVEEGEEVEIDYSIADREEIIPDDFGRIAAQ
                     TAKQVIYQRIREAEQDLFFKEYSTRVGEIVTGIVQQSDNRYTLVDLGRVEGLLPKSEQ
                     VPTERYEHGMRLKAVIKDVEQNPKGPPIILSRRSDELIRRLFELEVPEIADGLVEIVG
                     VAREPGYRSKISVVSHSDGVDPVGACVGPRGSRVRMVVSELRGEKIDIIPFNEEP"
     gene            complement(832..1281)
                     /gene="rimP"
                     /locus_tag="VFD74_04610"
     CDS             complement(832..1281)
                     /gene="rimP"
                     /locus_tag="VFD74_04610"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZK48863.1"
                     /translation="MKLTEDWVQDAIGDSIDGAEVVALEEAGSRRSRVVRLYVDHPDG
                     VTHELCARVSAVVGEALDQAGWGDSGYTLEVSSPGLDRPLRKPDHFRAQVGKRVYVKT
                     FAPVAGRKVWTGSLEAVLDGAVVVREGEEEATIAFDRITRAHVIFEF"
     gene            complement(1471..3183)
                     /locus_tag="VFD74_04615"
     CDS             complement(1471..3183)
                     /locus_tag="VFD74_04615"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZK48864.1"
                     /translation="MRASQLFMPTLKEDPADAEAVSHKLLVRGGFVRQFASGIYMFLP
                     LGWRVMGRVNRVIREEMDAIGALEFSMPTIQPADVWQQSGRYYDIGGEMFRLKDRGGR
                     DMVLAMTHEEAFTWLASRELRSYRELPQIWYQIQLKFRDEPRPKGGILRVREFMMKDS
                     YSFDVDVAGLEKSYAKHIVAYDNIFKRTGLDVHRVESDPGMMGGAQAHEYMAASAAGE
                     DRVVLCESCGYAANVELARSVAAAPQGVVPDFLEVVSTPGRRTIKEVSEFLRVPPSSL
                     IKSLLMVAGEEHVLVLVRGDQELHERKLERYLGKEVRPAHPEEVLETTGAEVGFVGPV
                     GVDVRTVADESLAPDGPGGARAYFAGANQADTHLTGVVLGRDVRPEWADLREAAPGEH
                     CVECGAALRVEQVIEVGNIFKLGTKYSVPLGATVLDEEGRETPLVMGSYGIGPARIAA
                     AAVEQCHDDRGIVWPKAIAPFDVHMVQVQAADEAQTEVARGLYDSLRAARWEVLWDDR
                     DERPGVKFKDAELVGCPVRITVGRRASEGFVEIEPRIGESSRREIAVTDVPAVVAELW
                     ECAP"
     gene            complement(3262..4344)
                     /gene="ispG"
                     /locus_tag="VFD74_04620"
                     /gene_synonym="gcpE"
     CDS             complement(3262..4344)
                     /gene="ispG"
                     /locus_tag="VFD74_04620"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014128721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZK48865.1"
                     /translation="MFSSQQVSVGGVLVGGAAPVVVQSMTNTPSTDVDATLAQIRALV
                     TAGAGLVRVAVPDEPAVAAFACLAAESPVPLIADVHFDHRLAIGALRAGAAAVRINPG
                     NLGGPARARAVAEVAAERGAVIRVGVNSGSLQRDLRSLEETDPAGALVESALRYCALF
                     EEVGFTELKVSVKSSSPTVTIEANRRLAQRIPYPLHLGVTEAGTPWAGSIRSAVALGA
                     LLAGGVGDTVRVSLTGDPVQEVLVAREILTSLGLIRGGPRVISCPTCGRTRIPLEQLA
                     LEVERRLSDLTQRLGLLDTPLEVAVMGCIVNGPGEARRADFGIAGGCGEGVVFAGGRP
                     IRKVPEDRLVDELFAAIEERLLRERP"
     gene            complement(4419..>4807)
                     /locus_tag="VFD74_04625"
     CDS             complement(4419..>4807)
                     /locus_tag="VFD74_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZK48866.1"
                     /translation="FTQTGGLFAMTFQGLAMMFTGEAPVTGSEGLAGPVGIVSVSSAA
                     FRGGYFMSFLAFISVQLAILNMLPLLPLDGGHVLFSLIEKLMGRAVSLRTFERVSAVG
                     ITLFLLLMLVATGNDITRVFGGGLGF"
BASE COUNT          873 a         1721 c         1396 g          817 t
ORIGIN      
        1 ggctcctcgt tgaacgggat gatgtcgatc ttctccccgc gaagctccga cacgaccatc
       61 cgcacccgag acccacgcgg accgacacat gcccccacag ggtcgacgcc atcgctgtga
      121 gacacgactg agatcttcga gcggtacccg ggctcccggg caacgccgac tatctctacg
      181 agtccatcgg cgatctccgg cacttcgagc tcgaagagac ggcggatcaa ctcatcgctt
      241 cggcgggaga ggatgatggg tggcccctta ggattctgct ctacatcctt gatcacggcc
      301 ttcaggcgca tgccgtgctc ataacgctcg gtgggcactt gttcgctctt tggaagaagg
      361 ccttcgaccc gtcccaggtc gaccagcgta tagcggttgt cggactgctg cacgatcccc
      421 gtgacgatct ccccaacccg cgtggagtac tccttgaaga acaggtcctg ctcggcctca
      481 cggatgcgct ggtaaatcac ctgtttggca gtctgtgcgg caatccgacc gaagtcatcg
      541 gggatgatct cttcgcggtc ggcgatggag tagtctatct ccacctcttc gccctcttcg
      601 acatcgagat ccggttccag gccgggcggg aaggtcagct ggaacacgtg tatctccccg
      661 gtgtcctggt cgatctcaac gtcggagaag ggcaccgcgt tcgccgtctt cttgtaggcg
      721 gagcggaggg cttcctccag cgcctcgatc aggataccga aggggatact cttctccttc
      781 tcgatctggc ggagagcatc gatcgtctct ctgttcattc cctctctcct actagaactc
      841 gaatatcaca tgcgcccgag tgatccgatc gaacgctatc gtcgcttcct cttcgccctc
      901 acgcaccacc accgcgccgt ccaagaccgc ttcgagcgat ccggtccaga ccttgcggcc
      961 cgcgaccggc gcgaaagtct tgacatagac tctcttgcct acctgggcgc gaaaatggtc
     1021 cggctttcgc agcgggcggt caagaccggg cgaactcact tccagcgtgt agccggaatc
     1081 gccccagccc gcttggtcca gagcctctcc caccacggcc gacactcgtg cgcacagctc
     1141 gtgggtcacg ccgtcgggat ggtccacata caaccgaacc acccgcgaac gcctgctgcc
     1201 tgcctcttcc agtgcaacga cctcggcccc gtcgatactg tcgccgatcg cgtcctgcac
     1261 ccaatcttcc gtcagtttca tcaaacctac cccatacaca acaaaagtgg ggcaccggct
     1321 cccacttctg cacactcctt cgatatcaga tgtatccgcg cacggattat agcacaggcc
     1381 ttaccaaccc gccatcgcct agcttccctc cggagagtat gacttgcgcc cgcttccgcg
     1441 cggcaccaac agatgcgacc ggacgtcctc ttagggtgca cactcccaga gttctgcgac
     1501 caccgccggc acatcggtca cggcgatctc ccgacggctc gactcgccaa tgcggggctc
     1561 gatctccacg aaaccctcag aagcccgccg gcctaccgtg atcctgaccg gacatcccac
     1621 gagttctgcg tccttgaact tgacgcccgg acgctcgtca cggtcatccc agaggacctc
     1681 ccaccttgct gcacggagcg aatcatagag tccccgagcc acctcggtct gcgcctcgtc
     1741 ggccgcctgg acctgcacca tgtgcacatc gaagggagct atggccttgg gccagacgat
     1801 gccgcgatcg tcgtggcact gctccacagc ggccgcggcg atgcgcgccg ggccgatgcc
     1861 gtaggacccc atcacgaggg gagtctccct gccctcttca tcgaggaccg tggccccaag
     1921 gggcacggag tacttggtgc caagcttgaa gatgttcccg acctcgatca cctgttccac
     1981 ccgcaacgcc gcaccgcact ccacgcagtg ttcgccgggg gcggcctcgc gcagatcggc
     2041 ccactcgggt cgcacgtcgc ggcctaggac cacgcccgtc aagtgtgtat ccgcctggtt
     2101 ggctcccgcg aagtacgccc gcgccccgcc ggggccgtcg ggcgccagac tctcatcggc
     2161 gaccgtgcgc acgtcgacgc ccaccggccc cacgaacccg acctcggcgc cggtggtttc
     2221 gagcacttcc tcggggtgag ccggacgcac ctccttgccc aggtagcgct ccagcttgcg
     2281 ctcgtgcagc tcttggtcgc cgcgcaccag cacgagtacg tgttcctcgc ccgcaaccat
     2341 cagaagagac ttgatcaagg aggaaggcgg cacccgcagg aactctgaca cctccttgat
     2401 ggtgcggcgg ccaggcgtgg aaacgacttc caagaagtcg ggcacgacac cctgaggcgc
     2461 ggccgccacc gatcgcgcga gttccacgtt ggcggcgtag ccgcacgatt cacacagcac
     2521 gacacggtct tcgccggcgg ccgaggcggc catatactcg tgggcctgcg cgccgcccat
     2581 catgcccgga tcgctctcca cccggtgcac atcgagtccg gtgcgcttga atatgttgtc
     2641 gtaggcgacg atgtgtttgg cgtagctctt ctccagaccc gccacatcga cgtcgaagct
     2701 atacgagtcc ttcatcatga actcacggac gcggaggatc cctccctttg gccggggttc
     2761 gtcgcggaac ttgagctgga tctgatacca gatctgaggg agctcacgat atgagcgtaa
     2821 ctcccgcgac gccaaccagg tgaacgcctc ctcgtgcgtc atcgcgagca ccatatcccg
     2881 gccgcctcgg tccttgaggc ggaacatctc gccgccgatg tcgtagtagc gaccgctctg
     2941 ctgccagaca tcggcaggct ggatggtggg catactgaac tcgagcgccc ctatggcgtc
     3001 catctcctcg cggatgacgc ggttgacccg gcccatcacg cgccagccca gcggaagaaa
     3061 catgtagatg cccgacgcga actgccggac gaacccgccc cgcaccagca gcttgtggct
     3121 caccgcctca gcgtcggccg ggtcttcctt gagcgtaggc atgaagagtt gagacgccct
     3181 catcgatccc tccctgttgg acacgaggtg cacatgttgc cgcactcgaa ccgctcacac
     3241 aagggcgtgt gcaccgccgg gtcaaggacg ctcgcggagc aaccgctcct cgatggccgc
     3301 gaacagctcg tccaccagcc gatcctcggg caccttcctg atcggcctgc cgcccgcgaa
     3361 gacgacgcct tcgccgcatc cgccggctat gccgaagtcg gccctgcggg cctcgccggg
     3421 accgttgacg atgcagccca tgacagccac ttccaacgga gtgtcgagaa gccccagtcg
     3481 ttgtgtgagg tctgagagcc tgcgctccac ctcgagcgcc aactgctcca ggggtatccg
     3541 cgtccggcca caggtgggac atgagatgac gcgaggcccg ccccgtatca gtccgagact
     3601 cgtcaggatc tcgcgtgcca ccagcacctc ctgcaccggg tccccggtca gcgacacgcg
     3661 taccgtatcg cccacgccgc ccgcaagaag ggctcccagc gccacggcgg aacgaatgga
     3721 gccggcccag ggagtcccgg cctccgtcac tccgaggtgg agtgggtagg gaatgcgttg
     3781 cgccaatcgc cggttcgctt cgatggtaac cgtcggcgac gaggacttca ccgacacctt
     3841 caactccgtg aacccaacct cctcgaagag tgcgcagtag cgcagcgccg actcgaccaa
     3901 cgctcccgcc ggatcggtct cctcgaggct ccggaggtca cgctgaagag aaccgctgtt
     3961 cactcccacc cggatgaccg ctccgcgttc cgccgccacc tcggcgacgg cccgcgcacg
     4021 cgccgggccc ccgaggttcc ccgggttgat gcgcacggcg gcggcgccgg ccctcagcgc
     4081 tccgatggcc agccgatggt cgaagtgcac atcggcgatg agaggcaccg gcgactccgc
     4141 cgccaggcaa gcgaaggccg ccacggcggg ctcatcgggc accgccacgc gcaccaggcc
     4201 cgctccggca gtgactagtg cacgtatctg ggcgagtgtc gcgtccacgt ccgtggatgg
     4261 cgtattggtc atcgactgca ccaccacggg ggcggcgccg cccacgagca ctccccccac
     4321 ggagacctgt tggctactga acacggcaca ccccgatgcg ggttccgcat gacgtctgcg
     4381 tcgcagcggg gatcagtgca tccagcccca tcatgtcgct agaacccgag tcccccgccg
     4441 aacacgcggg tgatgtcgtt gccggtagcc acgagcatga gcagcaggaa cagcgtgatg
     4501 cccaccgccg agacccgctc gaaggtgcgc aaggagacgg ccctccccat gagcttctcg
     4561 atcaagctga acaacacgtg cccgccatcc agggggagca agggcagcat gttgagaatg
     4621 gcgagttgca cactgatgaa tgccaggaag ctcatgaagt agccgccgcg gaaggccgcc
     4681 gatgacacgg acacgatccc cacaggcccc gccagaccct cgctccctgt gaccggagct
     4741 tcaccggtga acatcatcgc gagaccctgg aacgtcatcg cgaacaaccc gccggtctgg
     4801 gtgaacc
//
LOCUS       DASRMJ010000168         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_233033, whole genome shotgun sequence.
ACCESSION   DASRMJ010000168 DASRMJ010000000
VERSION     DASRMJ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233033"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            562..1050
                     /locus_tag="VFD74_04630"
     CDS             562..1050
                     /locus_tag="VFD74_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZK48867.1"
                     /translation="MDTHSDDAVLQVAIQLEELGRDFYESLASVSTDADMTRICRGLA
                     LAESHHRDVFSHLRQELAAQGRSILVADADLAEARRVLKEVVVPDRSQMLQALLEGRP
                     ADLLDRAAEMERNSITYYRGLAEHMSEKGVVLTVLEEELSHLRTLERLGFRLRAERAS
                     TS"
     gene            1135..2151
                     /locus_tag="VFD74_04635"
     CDS             1135..2151
                     /locus_tag="VFD74_04635"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009181923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZK48868.1"
                     /translation="MTTHDLTEGLSSNTTATGINHPSKLPDETVRGDMRYRRLGSTGE
                     EVSLIGLGGHHIGRQADENESVRIIRAAVDAGVTFMDNCWDYHDGLSEIRMGKALRGG
                     YREKVFLMTKIDGRTREAAADQVDESLRRLQTDVIDLLQFHEVIRLEDPDRIFAEGGA
                     MEAVRAAQKAGKVRFIGFTGHKDPLVHLRMLEVAKAHAFRFDAVQMPLNVMDAHFRSF
                     ERHVVPALIEEGIGVLGMKPMGDGRILDSNTVTPAECLRYAMSLPTSAVITGIDSMEV
                     LEQDLRVVKTFTALTPQERADLLRRTAEAAREGAFEGFKTTNGFDGTALNPSWLGAAD
                     SGPS"
     gene            2600..3655
                     /locus_tag="VFD74_04640"
     CDS             2600..3655
                     /locus_tag="VFD74_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZK48869.1"
                     /translation="MIIGVDPHKGSHTATAVSPTTSTAASSIRIDATLVGYRQLLLWG
                     KAFPERQWAIENAHGLGCHLAQWLIGRGETVIDVRTTATARVRELSRGGRRKNDVLDA
                     SAAAYVAAAQGDASSVQAEDETTVFALLEERRTNLAANRVRLVNQLHALLRDLIPGGA
                     RPALTAEAAAALLRGLRPVSTAERTRKELARELIGEIRAVDARLAQIATRMAEALHAR
                     GTRLLEVQGVGPVLAVRLLGRTGRPSRFPSAAAFASYTGVAPIEVASGDRTRHRLSRS
                     GDRQLNSALHLVAVTQVRMPNSVGRSYYDRKVAEGKSHNEAMRCLKRRIADHVWRLML
                     QDERLHARMTGSPAIAT"
     gene            complement(3678..>3986)
                     /locus_tag="VFD74_04645"
     CDS             complement(3678..>3986)
                     /locus_tag="VFD74_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HZK48870.1"
                     /translation="IRYTARLEDAGAVTSVGSRGDAYDNALAETINGLYKTELIRKRG
                     PWKTLEDVEFATLEWVDWFNNRRLLEYTGYIPPAEFEEMYYREHSLVEDEGLKQMSLQ
                     "
BASE COUNT          745 a         1255 c         1288 g          698 t
ORIGIN      
        1 gccctccgat tcggcccacc tgagagaacc gggtcggggg gctcttcagc gcccacggca
       61 ggtcggacga gcgatcgtcg cgaggctcct ctacctgtcc gacaaagggc gtttcttgca
      121 gcccacgggc tcaggtgaag cttttcttct ggcaccagca ccctggcggc ctctcgacgg
      181 gtccgacgcg gtgcgagtaa ggcactctga aacgagcacc atgacatcat ggtggtcgtt
      241 cccctgcggt aaccaacggg acagagtgac cacgccattc gcgcctggtg cggctccctg
      301 ccggcccaga tcctctttgg tgtttgggtt cgatcagttg gagcaacaga tcgactgcga
      361 gtccccaaga gacgaagcgc aactcccccg gggttttctg ggaaagtgaa tgggtactaa
      421 gggggagtag aaccgcttcg gacctgttct tgcgtggcgc gcaaagatcc caacgtcgcc
      481 tcgcactaag ctcggcgatt cccttgtccg attatgagag agccagcacg ctatgcctgc
      541 tagataagga aggaagggca gatggatact cacagtgatg acgctgttct tcaagttgcc
      601 atccagctgg aggaactcgg tcgggatttc tacgagtctc tggccagcgt tagcactgac
      661 gctgatatga cccgtatctg ccggggcctc gctctagcgg agtctcatca ccgggatgtc
      721 ttcagtcact tgcgtcagga gctggccgct caaggtcgca gcattctcgt tgccgacgcc
      781 gatctggctg aagcgcgccg agtcctcaag gaagtggtgg ttccggatcg cagccaaatg
      841 ctgcaggcgc tgttggaagg ccggccggct gacctgttgg accgtgccgc cgagatggaa
      901 aggaactcga tcacctacta ccgcggcctg gccgagcaca tgagcgaaaa gggagtggta
      961 ctgactgtat tggaggagga gctaagccat ctgcgcacct tggagcggct aggctttcga
     1021 ctgcgcgctg agcgggcctc gacctcctga tggttcgaaa gtaaagagtc acttttcgcg
     1081 cgcatgttgg ccggcgccct acacctgcct acccctgccg aaagggagtc ccgcatgacg
     1141 acgcacgatt tgaccgaggg cctaagcagt aacaccacag cgacgggcat caaccatccc
     1201 tcgaagcttc cggacgagac agtccgcggc gacatgcgct atcgccggct gggtagtacg
     1261 ggcgaggagg tgtcgctcat cggattgggc ggtcaccaca ttggccgaca agcagatgaa
     1321 aacgagagcg tcaggatcat tcgcgccgct gtcgacgccg gcgtcacttt catggacaac
     1381 tgttgggact accacgatgg cctgagcgag atacgcatgg gcaaggcctt gcggggcggc
     1441 tatcgcgaga aggtattcct gatgaccaag atcgacggtc gcactcggga ggcggccgca
     1501 gaccaagttg acgaaagcct tcgccggctt cagacagacg ttatcgacct tctgcagttc
     1561 catgaggtca tccgtctgga ggatccagat aggatctttg ccgagggcgg ggcgatggag
     1621 gctgtccgcg ccgcacagaa ggcgggcaag gtgagattca tcggcttcac cgggcacaag
     1681 gacccgcttg tccacctccg gatgctcgag gtggccaagg cgcacgcgtt tcggttcgat
     1741 gcggttcaga tgccgctcaa cgtaatggac gcccacttcc gtagcttcga acggcacgtc
     1801 gtcccggcgc tcatcgaaga gggcatcggc gtcctgggca tgaagccgat gggcgacggg
     1861 cggattctgg acagcaatac ggtgaccccg gccgaatgcc tccgctacgc catgagcctg
     1921 ccgacctctg ccgtgatcac cgggatcgac agtatggaag tcctggaaca ggacctgcga
     1981 gtggtcaaaa cgttcacggc gctcacccca caagagcgcg cggacctgct caggcgaaca
     2041 gccgaggcgg cacgcgaggg cgcattcgag gggttcaaga ccacgaatgg gttcgatgga
     2101 acagccctga acccgagctg gctcggtgcc gccgacagcg gcccgagctg agcagggcgc
     2161 gcgagcgaga cggcggcttc gttgccggcc cggctcctac cggatggctt caaacaccgc
     2221 cgagatgtcc tcatcgccgt gcccggtgac acgtgcgcgt ttgaaggtct cattcactgc
     2281 ggctgtacag tggagcggct gcccagacct ttctgacagc tccacggcaa ctgacagctc
     2341 cacggcaagg cggagtgaac cgccgactta ttgtggaagc agcggtgcgc aacgcgcaag
     2401 agcctgttag cgttccgtag gtcatcggcc ggggtgactc gtgaaccgcc tgccgcccgg
     2461 taagggctgg ctctcccggg gtgtgtcatc cggtcggtga ccggctgcgg tctgactcaa
     2521 cagaggcgtg atccactcgg gctcccgaag tcggcatgtc gaccgtggtc tccttgtcac
     2581 agaggaagga ggaacggtcg tgatcattgg agtcgacccg cacaagggct cccataccgc
     2641 tacggcggtc agcccgacaa caagcaccgc ggcgtcatcg attcgcatcg acgccacctt
     2701 ggtagggtac cggcagctgc tcctttgggg caaagccttc cccgaacgcc agtgggccat
     2761 cgagaacgcc cacggcctcg gttgccacct ggcgcagtgg ctcatcggcc gcggtgagac
     2821 cgtgatcgac gtccgcacca ctgcgacggc acgagtacgt gagctctccc gcggcggccg
     2881 ccgcaagaac gacgtcctgg acgcatccgc ggcggcgtat gtcgccgccg cgcagggcga
     2941 cgcctcgtcg gtccaagccg aggacgagac caccgtgttc gccctgctcg aggaacgccg
     3001 gaccaacctg gccgctaacc gggtcaggct cgtcaaccag ctccacgccc tgttgcggga
     3061 tctgattccc ggaggggcca ggccggcgct cacagccgaa gctgcggcgg cactcttgcg
     3121 gggccttcga cccgtctcga ctgccgagcg cacacgtaaa gagctcgccc gtgagctcat
     3181 tggggagatc cgcgccgtcg acgctcgact ggcccagatc gctacgcgga tggccgaggc
     3241 gctccacgcc cgcggcaccc ggctccttga ggtgcaggga gtcggcccgg tgctcgccgt
     3301 tcggctgctt ggccggacgg gacgcccgtc gcggttcccc agtgccgcgg cgtttgcgag
     3361 ctacacgggg gtcgctccca tcgaggtggc cagcggcgat cggacccgcc accggctctc
     3421 tcggagcggc gaccgacaac tcaactccgc cctgcatctg gtcgcggtca cccaggtgcg
     3481 gatgccgaac agcgtcgggc gctcctacta cgaccgcaag gtggcggagg gcaagagcca
     3541 caacgaggcc atgcgctgcc tgaagcgacg gatcgcggat cacgtctggc gcctcatgct
     3601 gcaggatgaa cgactccacg cgaggatgac cgggagccct gcgatcgcca cttgacaaac
     3661 acagaggcac cccgggttca ctggagactc atttgcttga gtccctcgtc ttcaacgagg
     3721 gagtgctcac gatagtacat ctcctcgaac tccgcaggtg ggatgtagcc ggtgtactcg
     3781 agaagccggc ggttgttgaa ccagtccacc cactcaagtg tggcgaactc cacgtcttcg
     3841 agcgtcttcc aggggccgcg ctttctgatg agctcggtct tgtagaggcc gttgatggtc
     3901 tcggccagtg cattatcgta ggcatcaccg cgggagccca ccgaggtgac cgccccagcg
     3961 tcctccaggc gggcggtgta gcggat
//
LOCUS       DASRMJ010000169         8055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_13238, whole genome shotgun sequence.
ACCESSION   DASRMJ010000169 DASRMJ010000000
VERSION     DASRMJ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13238"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..2057
                     /gene="mnmG"
                     /locus_tag="VFD74_04650"
     CDS             126..2057
                     /gene="mnmG"
                     /locus_tag="VFD74_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZK48871.1"
                     /translation="MSAAMDYDVVVVGAGHAGCEAALAAARMGCRTALITLNIDKIAL
                     MPCNPAIGGVGKGQLTREVDALGGEQGRNTDRAYVQIKMLNTSKGPAVRALRAQADKR
                     LYEDWMKVALFGTEGLDVLQGEVADLLVSRETVSGVTLVDGRRLSARAVVLATGTFLR
                     GKVVVGQRTYPAGRVGELPAAFLSGGLVKLGIEIGRFQSATPPRIDGRSIDRTKMLVQ
                     AGDEDVSGFSHWESPRPRKQMPCYLTHTTEAVHDVVAEYLDLSPIKTGSVSGRGPRYC
                     PSIDRKVINFPDRDRHPVFVEPEGARTHEMYLQGLTTSLPVFVQEKIIQATPGLEHAR
                     LVRPGYAVEYDYIVPQGLTLALETKAVSGLFSAGQINGTSGYEEAAAQGMIAGINAAL
                     GVKGEPPLILGRDEAYIGVLVDDLVTKGVDEPYRMFTSRAEYRLLLRHDNAVERLSHI
                     GHALGLISGEQLARVNERIELVERAAETLETMRIRRSERSDALLEGLGTTGITESQTA
                     TQMLRRPQVTFDVLLSLMEGPETEWLSGLAPEIVEQIEIRAQYDGYIQRQMDEIARRK
                     STEGTAIPVDLAIADLVGITVEAKDKLARLKPMTLGQASRIAGVSPADISVLLMHIHR
                     HRSTRGRDDDVSARSTSSD"
     gene            2097..2804
                     /gene="rsmG"
                     /locus_tag="VFD74_04655"
     CDS             2097..2804
                     /gene="rsmG"
                     /locus_tag="VFD74_04655"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZK48872.1"
                     /translation="MELRERAAYADWGLSEPDVEKLGIFGDLLLAARSNVTAIRDPHE
                     IELSHFLDSLSLLDVAEIRGASQIVDVGSGGGLPAVVLALALPASRVTAIDSVAKKCA
                     FIEEARTRLGLENLRVVCGRAEDVGRTEAREAFDVATARAVASLAVLAELTVPLIRTG
                     GVFVASKAALSDQERMEGEAALAILGCDRYDLRRARSFAQAENRWLFLAYKDRSTPAQ
                     YPRRTGVPAKRPLRAQR"
     gene            2812..3606
                     /locus_tag="VFD74_04660"
     CDS             2812..3606
                     /locus_tag="VFD74_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK48873.1"
                     /translation="MDTLEEGRGDRLIYAVANQKGGVGKTTTAINLAATLAQAGERVL
                     LIDLDPQANATTGLGLRVDDGRPSMFEVLLGEETLGKVLLPTPVSGLWVAPSSRDMAG
                     ASVVLPGLERREYLLRDALAALGPEDAFSFVFIDCPPSLGLLTVNALVAADEVVVPVQ
                     AEYYALEGLAQLLDTVEALRGRLNPGLQVAGLVLTMADVRTRLAREVENELRSHFPGL
                     TFKTVVPRNVRLAEAPSFGLPVSLLDPRCPGSDAYFDLAKEVIARD"
     gene            3599..4603
                     /locus_tag="VFD74_04665"
     CDS             3599..4603
                     /locus_tag="VFD74_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZK48874.1"
                     /translation="MTSRKRGGLGRGLAELLGEGASAEAANRVRRIPGDAGGPAVPDG
                     SAPDSRLKQAASEAPTETIAAGTAARAGVLELPTASIRPNPKQPRRDIEAGPLEELAR
                     SIESVGLIQPVIVRRAGDGYELIAGERRWRAAQLAGFTVIPAILREASDVESLQLALV
                     ENVVRQDLNVMDEAFALQVLLEDLGVTQEALAAQVGKSRSAIANKIRLLELPGPVQEM
                     LVEGGLAEGHGRALLGLRSRGDQLRLARKAVQQGLSVRVVENEVRRANESASGEGRPS
                     KASTISISSETLDAVRDGVYGTLAVMPRIRLGRTGGVVELPFEDEAALRGLLERLGRV
                     "
     gene            4829..5611
                     /locus_tag="VFD74_04670"
     CDS             4829..5611
                     /locus_tag="VFD74_04670"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZK48875.1"
                     /translation="MQHYIGRKYGLPAWILAILLVAAVGVVAVGCADDAEPAVTGAGG
                     SVQPIDSITVSGEGLVKAGPDEATLTIIVETDAPDAPQALDANSTQMTQVLERLKAEG
                     LAEESLQTANVAVYPNRRWDPQSGEETIEGYRAQNSIRVTLTDLTKVGDVFTAATEAG
                     ANNISGPEWRLADDSAAVTEALDKAFASARSKAETLAKAAGLKLGAVLTMQESSVAIP
                     PIMYGTRTGGDAASMESLKSTPVNPMDLEVQASVTLTFRLEK"
     gene            5833..6126
                     /locus_tag="VFD74_04675"
     CDS             5833..6126
                     /locus_tag="VFD74_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48876.1"
                     /translation="MELRIEKTPSSSGWQVHVVVALDRQETSELFLAGDKLISWPTDG
                     MRSSGSGPMNRSNMFLSEMVARPDGLILTYDDAELAERTGKILEHQIRSALGG"
     gene            6133..6465
                     /locus_tag="VFD74_04680"
     CDS             6133..6465
                     /locus_tag="VFD74_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZK48877.1"
                     /translation="MSSEDPIYELVRSYLDIDGVLAAVLVSDQGLVVNGAMNDAVDVD
                     TISALIAETVASAQRVGVESAVGRMETMILEFEKLSLLLAPFDHELMLALVGRPGTFA
                     RSAGLVGS"
     gene            complement(6581..7024)
                     /locus_tag="VFD74_04685"
     CDS             complement(6581..7024)
                     /locus_tag="VFD74_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48878.1"
                     /translation="MNRRWAVVVIVVVALLLLLPMPVAERYTSPTQDGQYLMDPIHAY
                     RFAFAAATVSPSSVLNTSGEALAKAKSLFSGSEYRPNKVELLFLGGEGSYEYVAKDGT
                     NLFVPSPGQFIWEVWGQTPGESPGARADVIALLDYKTGELLDSVK"
     gene            complement(7110..>8055)
                     /locus_tag="VFD74_04690"
     CDS             complement(7110..>8055)
                     /locus_tag="VFD74_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48879.1"
                     /translation="VSPAGQPTGRPITLGATRTSDHFVVRSASASTAEQTLTLAEHAW
                     DLLAPHFEFLPSEPVTIVVVEDTAEYERIQPAAMTRGFATFGGDHIYLRGADLDQEVV
                     THELVHILLGKNIRRGLAVPDWFNEGFAQFMSGADSHRREVFYLAASGRLLPLPELDR
                     LDALRGPDRELPTIQGLAVVGFFVDEYGRDRLWDLVSRLSHARTFSQALLDTYGRSDL
                     ELSHEWQAYADTEYGLLSVVGLETLGTAMLGLLSLLAAVVWFATRIRLRARPASPLDL
                     SPWEIESAERAAAMLDTGGGAGDAQGARGEDSGDRRTT"
BASE COUNT         1437 a         2467 c         2765 g         1386 t
ORIGIN      
        1 gtgttgcccg gagtgtgggt ggccggccaa gccgccggtg cttcgggtta cctggggagc
       61 ctgtcgagtg ggtggcgcat cgggcaagcg atggcggatg ctcttctggc cggcaaggcg
      121 ggaatatgag cgcggcgatg gactacgacg tcgtcgtcgt tggagcaggg cacgctggat
      181 gcgaagccgc tctggccgcc gcacggatgg ggtgccgcac tgccctgatc accctgaaca
      241 tcgacaagat cgcgctcatg ccctgcaatc ctgccatcgg cggcgtgggc aaggggcaac
      301 tgactcgcga ggtcgacgca ctcggtggtg agcaggggcg caatacggac cgggcgtacg
      361 ttcagatcaa gatgctgaac acgtccaaag ggccggcggt gcgggcgctg cgggcgcagg
      421 cagacaagcg tctctatgaa gactggatga aggtggctct gttcggaacc gaagggcttg
      481 atgtcctgca gggcgaagtc gccgatctcc ttgtttcacg tgaaacagtg agcggcgtca
      541 ccctggtcga tgggcgaaga ctgtccgcgc gggcggtggt tctcgctacc ggaacgtttc
      601 tgcgcggcaa ggttgtcgtc ggacagcgca cctaccctgc cggtcgggtc ggagagttgc
      661 cggccgcgtt cctatcgggt ggtctggtga agctgggcat cgagatcggt cggtttcagt
      721 cggccactcc tccccgcatt gatgggcgtt cgatcgatcg aacgaagatg ctggtccaag
      781 ccggggatga agacgtgtct gggttctcgc actgggagtc ccctcgtccg aggaagcaga
      841 tgccctgcta cctcacgcac accacggaag ccgtccacga tgtcgtggcg gagtacctgg
      901 atctgagccc catcaagacg ggttccgtca gtggccgggg ccctcggtac tgtccctcga
      961 tcgaccgcaa ggtcatcaac ttccccgatc gcgaccggca cccggtcttc gtcgagcctg
     1021 agggcgcgag aacccacgag atgtacctgc aggggctcac aaccagcctc ccggtgttcg
     1081 tgcaggagaa gatcatccag gccacgccgg ggctggaaca cgcgcggctt gtgagaccgg
     1141 gctacgcagt cgagtacgac tacatcgtgc cgcagggact tacgctcgcg cttgagacca
     1201 aggcagtctc gggcctgttc tccgcggggc agatcaacgg cacctcggga tatgaggaag
     1261 ccgctgcgca ggggatgatc gccgggatca acgccgccct cggggtgaag ggggagccgc
     1321 ccctgatcct cggccgcgac gaggcctaca tcggtgtgct tgtggatgac ctcgttacca
     1381 aaggggtgga cgaaccgtat cgtatgttca cctcgcgtgc ggaatacagg ctgctactgc
     1441 ggcacgacaa cgccgtcgag cgtctcagcc acatcgggca cgcgcttggc ctcataagcg
     1501 gcgagcagtt ggctcgagtc aacgaacgca tcgagcttgt ggagcgcgcc gccgagacac
     1561 tcgaaaccat gaggatccga cggagcgagc gaagcgacgc cctcctggag ggcctaggga
     1621 ccactggcat cacggaatct cagacggcga ctcagatgct acgccgtccg caggtgacgt
     1681 tcgacgttct tctctctctc atggagggcc cggaaacaga gtggctctca ggccttgctc
     1741 ccgagatcgt ggagcagatc gagatcaggg cccagtacga cggatacatc cagcggcaga
     1801 tggacgagat cgcccggcgc aagtccaccg aaggcacagc cataccggtc gatctggcca
     1861 tcgcggacct tgtggggatc acggtcgagg ccaaggacaa gcttgcacgc ttgaagccga
     1921 tgaccctcgg gcaggcgtca cgcatagcgg gtgtgtcccc ggcagacatc tccgtcctcc
     1981 tgatgcacat acatcggcac cgatccacgc gcggccgcga cgacgacgtc tcggcacgtt
     2041 cgacctcctc agattgaacc ggctgtggtg accagcgagc ttcggagcca ggcagcatgg
     2101 aactaagaga acgagcagcc tacgcggact gggggctgag cgagcccgat gttgagaagt
     2161 tgggcatctt cggcgatctg ctgttggcgg cacggtccaa tgtcaccgcc atccgcgacc
     2221 cgcacgagat cgagctgagt cacttcctgg attctctctc actactggat gttgccgaga
     2281 ttagaggggc ttcgcagatc gtggacgtgg gctcaggcgg cggtcttccc gcggtggtcc
     2341 tcgcccttgc cctacctgcc tcgcgtgtta cggccatcga ctcggtggcc aagaagtgcg
     2401 cattcatcga ggaagcgcgc actcgcctcg ggttggagaa tctgagagtg gtgtgtgggc
     2461 gcgctgagga tgtcgggcgg accgaagcca gggaggcgtt cgacgtggcc acggcaagag
     2521 ccgtggcttc gttggccgtg cttgccgaac ttactgtgcc gctgatcagg accgggggcg
     2581 tcttcgtggc ctcgaaggcc gcactctccg accaagagcg tatggagggt gaggccgcgc
     2641 tcgctatact gggatgcgat cggtacgacc tgaggcgagc gcgctccttc gcccaggctg
     2701 agaaccgatg gctcttccta gcgtacaagg atcggtcaac gccggctcag tacccgcgtc
     2761 gaaccggcgt gcctgcaaag cgaccgctcc gggcgcaaag atgagtgcca ggtggacacg
     2821 ctcgaagagg ggcgggggga ccgcttgatc tatgcggtag cgaatcagaa aggtggagtc
     2881 ggcaagacga cgactgccat caacctcgca gcgacgttgg cccaagcggg cgagcgggtg
     2941 ctgctcatcg atctcgatcc gcaggcgaac gcgaccaccg ggctgggtct gcgcgtggac
     3001 gacggccgac cgtcgatgtt cgaggtcctg ttgggggaag aaacgctcgg aaaggtgctg
     3061 ctgcccaccc cggtcagtgg tctgtgggtg gcgccgtctt ctcgggacat ggcgggtgca
     3121 tcggtagttc taccgggact cgagcgcaga gagtacctgc tgcgcgacgc actcgccgcc
     3181 ctcgggcccg aagacgcgtt ctcgttcgtg tttatcgact gtccgccttc tctgggactc
     3241 ctgacggtga acgcactcgt cgcggccgac gaggtggtcg tgccggtcca agccgagtac
     3301 tacgcactcg agggactggc gcagcttctt gataccgtcg aagcgctgcg agggcgtttg
     3361 aaccccggcc tgcaggtggc gggtctggtg ctcaccatgg ccgacgtgcg cacgcggttg
     3421 gcgagggaag tcgagaacga gctgcgctcc cacttccccg gtctcacatt caaaacggtg
     3481 gttccgcgga acgtccgtct ggccgaggcc ccaagtttcg ggttgcctgt ctccttgctc
     3541 gatccccgct gtccgggatc cgacgcgtac ttcgacttgg cgaaagaggt gatcgcgcgt
     3601 gactagccgg aagaggggcg gcctcggtcg ggggcttgct gaactgctgg gagagggtgc
     3661 gtcggcggaa gcagcgaacc gcgtccggag gatccccggc gacgccggcg ggccggcggt
     3721 gcccgacggt tcggcgccgg actctcgatt gaagcaggcg gcgagcgagg caccaaccga
     3781 aacgatcgcc gccgggacag ccgcgagagc gggagtgctt gaactaccca cagcgtcgat
     3841 acgaccaaat ccgaagcagc cgcgccgcga tatcgaagcc ggtccacttg aggaattggc
     3901 gcgctccatc gagagcgtcg ggttgatcca gccggtgatc gtacgccggg ccggtgacgg
     3961 ctacgaactc atcgccggtg agcgccgctg gagggcggct caattggccg ggttcacggt
     4021 catcccggcc atcctgcgtg aggcgtcgga cgtcgagagc cttcagttgg cgctggtgga
     4081 gaacgtagtc aggcaagatc tgaacgtcat ggatgaggct ttcgccctcc aggtcttgct
     4141 ggaagacctc ggcgtgaccc aggaagcgct cgcggcgcag gtggggaaga gtcgctccgc
     4201 gatcgcgaac aagatcaggt tgctcgaact gccggggccc gtgcaggaga tgctggtcga
     4261 gggaggtctc gccgaaggcc atggtcgtgc tcttctcggg cttcgttcgc gcggcgacca
     4321 gctgagactt gcccggaagg ccgtgcaaca gggattatct gtgagggtgg tcgagaacga
     4381 ggtgcggcgt gccaacgaat ccgcttcggg agagggaaga ccctcaaaag cgtccacaat
     4441 ctccatctcg agtgagactc tcgacgccgt gcgggacggc gtgtacggta cgctcgcggt
     4501 gatgcccagg atccgattgg gcaggaccgg aggcgtggtg gagctcccat tcgaggacga
     4561 ggccgcgctg cgcggactgc ttgagcggct ggggcgggtc tagataccac cggcactgcg
     4621 tgcccggctc tcagctcagg tcgtgggccg ggctggaatc gtccctgcgg ccggaaccac
     4681 cttgtggcgc tccgcgtctc agtccccgga acgagcgcga acgggtcgtg ccgcgatccg
     4741 gggcctcttg gccccaaggc gttcgtagcc ccgattgtgg accgctctcc gaacccactg
     4801 accgtcgatg aagaggaggc ctgtagagat gcaacactat ataggacgga agtatgggct
     4861 gccggcctgg attctagcca ttctcctggt cgccgccgtg ggcgtcgtcg ctgtcggctg
     4921 cgccgacgac gcggaacccg ccgtgaccgg agccggtggc tcggtgcagc ccatcgattc
     4981 gatcacggtc tccggagaag gtctggtcaa ggccggaccg gacgaagcga cgttgaccat
     5041 catcgtcgag accgatgcgc cagacgcccc ccaggcgctg gatgcgaact ccacgcagat
     5101 gacgcaagtg ctggaaaggt tgaaggccga aggactggcc gaggagtcgc tgcagaccgc
     5161 caacgtagcc gtgtacccca accgcagatg ggatccgcag tccggtgagg agaccatcga
     5221 gggctaccgc gcgcagaact ccatccgagt tacgctcacc gacctgacaa aggtgggcga
     5281 tgtcttcacg gcggcgacgg aagcgggggc caacaacatc agcggtccgg agtggcggct
     5341 cgccgacgac agcgcagcag tcactgaggc gctcgacaag gcgttcgcga gcgctcgaag
     5401 caaggccgag acgctggcca aagcggctgg tctgaagctg ggggccgtgc tcaccatgca
     5461 ggagagctcc gtcgcgatcc cacccatcat gtatggcacc aggactggag gcgacgccgc
     5521 atccatggag tctctgaagt ccaccccggt gaacccgatg gatctcgagg tccaggcttc
     5581 ggtgacgctc accttccggc tcgagaaata ggccgactgg ggcgcggcgc atgccttcgg
     5641 tgacagcgcg ccgcaccgga gtccgacccc agcccgaatg aagtccgagg agccccgctt
     5701 cggcggggct cctcgcgtcg ttcctgattt ccgtagatga gtggcgcaga tgccttcctc
     5761 ggcggcgccg gtggtgcctc cgcaagcgcc ttcgcaaccg cccccgggat gtccgcctgc
     5821 tatgatgcgg gcatggagtt gcgtatcgag aagaccccct ccagctcggg ctggcaggtc
     5881 catgtggtgg tcgcgttgga tcgtcaagag accagcgaac tgttcctcgc gggagacaag
     5941 ctgatctcgt ggcccacaga tggcatgcgc tcctcgggct ctggccccat gaacaggagc
     6001 aacatgttcc tgtctgagat ggtcgcccgt cctgacgggc tgatactgac ctatgacgac
     6061 gcggagctcg ccgagcggac cggcaagatc ctcgagcacc agatccggtc cgccttggga
     6121 gggtagtcgc gcgtgagtag tgaagacccc atctacgagc ttgttcgcag ctacctggat
     6181 atcgacgggg tgctcgctgc ggtgctggtc tcggaccagg gactcgtcgt caacggtgcg
     6241 atgaacgatg ctgtcgacgt cgataccatc tctgccctga tcgcggagac ggtggcgtcg
     6301 gctcagcgag tcggggttga gtccgcggtc ggcaggatgg agaccatgat cctggagttc
     6361 gagaagctct cgctcctgct ggcaccgttc gaccacgaac tcatgctggc gttggtgggg
     6421 aggcccggca ccttcgcgcg ctcggcgggt ctggtcgggt cgtaggttcc ggtgcgtggg
     6481 ccacgggccc acatcgtgcc ggatacgcga ctgccggcgc ccgactatcg gcgcccgact
     6541 atcggccccc gactatcggc gcccgactat cggcgcccga ctacttcaca gagtcgagga
     6601 gctctccggt cttgtagtcc agcagcgcga tgacgtcggc gcgcgccccg ggcgactcac
     6661 ccggggtctg cccccacacc tcccagatga actgacccgg tgagggaacg aacaagttcg
     6721 tcccgtcctt ggccacgtat tcgtaggacc cctcgccccc caagaagaga agctccacct
     6781 tgttaggtct gtattcgctt cccgagaaga ggcttttggc cttggccagg gcttcgcctg
     6841 acgtgttcag gaccgacgat gggctgacgg tggccgcggc gaaggcgaag cgataggcgt
     6901 gtatcgggtc catcagatac tggccgtctt gcgtcgggga cgtgtaccgt tcggccaccg
     6961 gcatgggcag caagagaagc agagccacga ccacgatgac cacaactgcc caacgacggt
     7021 tcattctttt cgcccgatct cgttcgggtt gcctggcttc ctttgcagcg gagcggcgat
     7081 ccagcgccgg agcatcaggg tatcagagat caagtggtgc gacgatcacc cgagtcttcc
     7141 cctcgggcgc cctgggcatc cccggcccca ccgcccgtgt caagcatcgc ggcggctctc
     7201 tctgcggact cgatttccca aggagagagg tcgagcggtg acgcggggcg tgcgcggaga
     7261 cggatcctgg tggcgaacca gactacagcc gccaacagag atagcagacc caacatggcg
     7321 gttccgaggg tctccagacc gaccaccgag agcagtccgt actcggtgtc ggcgtacgct
     7381 tgccactcgt ggctcaactc caaatcagag cggccgtacg tatcgaggag cgcttggctg
     7441 aacgtgcggg catgtgagag gcggctcacc agatcccaga gcctgtcccg gccgtactcg
     7501 tcgacgaaga atcccacaac ggcgagcccc tggatcgtgg ggagttcgcg gtctgggccg
     7561 cgaagcgcgt cgaggcggtc gagctccgga agcggtagga gtcgtccgga agccgccaag
     7621 tagaagacct cccggcgatg gctgtcggcg ccggacatga attgagcgaa cccttcgttg
     7681 aaccagtctg ggacggcgag tccacggcgg atgttcttgc cgaggaggat gtggaccaac
     7741 tcgtgggtga cgacctcctg gtccaggtcg gcgccgcgca gatagatgtg gtctcccccg
     7801 aaggtcgcga agccacgagt catggcggcg ggttggatgc gttcgtactc cgccgtgtcc
     7861 tccaccacga cgatggtcac cggttcggag gggaggaact cgaagtgcgg tgcgaggagg
     7921 tcccaagcgt gctcggccaa agtcagggtc tgctccgccg tcgaggctga ggcggatcgc
     7981 accacgaagt gatcggaggt gcgcgtcgcc ccgagggtga tgggccggcc cgtcggctga
     8041 cccgccggcg aaacg
//
LOCUS       DASRMJ010000170         7463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_49126, whole genome shotgun sequence.
ACCESSION   DASRMJ010000170 DASRMJ010000000
VERSION     DASRMJ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49126"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /gene="ligA"
                     /locus_tag="VFD74_04695"
     CDS             complement(<1..1250)
                     /gene="ligA"
                     /locus_tag="VFD74_04695"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZK48880.1"
                     /translation="MTAGPGPAYDQTAARVLELRALIRRHDHLYYGLDSPIIADAAYD
                     DLLRELRGLETQFPDLLTGDSPTQRVGGEPLEAFGRIRHLEPMLSLANAKDEAELEAW
                     YRRVVRLAEETGFAPESLRFTLEPKIDGLAVSLRYEHGVLVSGATRGDGSVGEDVTQN
                     LRTISAIPLVMLSAQGLSSTEVPVPDLVEVRGEVYLPLEAFERLNESRRANDESTFAN
                     PRNAAAGSLRQLDPRVTASRPVSMWAYGVVLAGTSLLDALGEGAPETWSGGQWQVLEW
                     LRARGFRVSPDVRRVAGLDEILAVCRDWERRRASLGYDIDGVVVKIDDRTVQLALGAA
                     GRDPRWAVAYKFAPSTAQTRLLDIRINVGRTGMLNPYAVLEPVEVGGVTVGQATLHNE
                     DDIRRKDLRVGDTVIVQRAGDVIP"
     gene            complement(1247..2974)
                     /gene="surE"
                     /locus_tag="VFD74_04700"
     CDS             complement(1247..2974)
                     /gene="surE"
                     /locus_tag="VFD74_04700"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZK48881.1"
                     /translation="MMSTEHPLVHLPRSGLTILLTNDDGIDSPGLFAVKRALDPLGDV
                     HVIAPDRNRTGAARSITMRAPLWVEERILPDGSMGHSTDGTPVDCVRLAALGFLERRP
                     DLIVSGINLSGNLGDDITYSGTVAAAFEGIMLGIPSLAVSADGYHDGYDLSVPARVSR
                     LVVESILEKGFPAEALLNINCPDLDWEALSGIRLTTLGKRIYGDRVQLQESRGSRRRY
                     FVYGDDLDYHNEVGTDFEAVGGGFVSVTPIHFHLTAHDVLRRMEDWRLDLADAVTEPA
                     AGAWAFPGEGRERAEPGDAGIPGSHAESGGDAEPGDAAPAVGDAAMGRAAETATGNET
                     ADETAYETAASSSSPSLSPLEPAPRVVMFDLDGTVLDSVELIVDSFEHAILTVLGYRS
                     TREDAVHNVGRPLLEQMESFDEVRAQELVDAYRDYNHREHDRRVRLYPGMADLLLRLR
                     ARGVRLALVTSKSRHTTEMAFRVTGVGELFEAVVCAGETTYHKPHAEPLLRALDSLGA
                     APESAVYVGDSPYDLQAATAAGLRSVGVAWGVFPEGDLAAESPDRMVRTMAQLEVVLS
                     GSDTLTGDS"
     gene            complement(3056..4237)
                     /locus_tag="VFD74_04705"
     CDS             complement(3056..4237)
                     /locus_tag="VFD74_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZK48882.1"
                     /translation="MGVLFVRFEHWGHTSRLYGWREIQSVYERASGAAMGMLGHGLRQ
                     LDLPADLGLRGEGFAILLSGPRESADLGLEIVDLVADRVADLVRELLSDGVAAELMER
                     TTIEVGAGLVNRPEAAGTLEDALVAGLVEAERSAREKHAARLVEMGDHLRSALDEGRL
                     TTLFQPIAAIESGKTVAFDAVVQGPLYSNLQHGDVVFDVAERTGQELRVLDLYHHATL
                     SAVDDALDGAEQLVLRATAEELLESAVRLMSLLYHREPARITPANVLFLVGSDRLADH
                     FPAGLSAWRSVGDMGFRLCVDLSPNRPLPLDYLKELSPDVVRVSGRLVRDVHRHQDEF
                     EYLLMLSRFTTRHDMRILAADCIDRREFTTLRRAGVSYVQGEFVAPCVPTLSRPELIL
                     P"
     gene            complement(4505..4987)
                     /locus_tag="VFD74_04710"
     CDS             complement(4505..4987)
                     /locus_tag="VFD74_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HZK48883.1"
                     /translation="MRSASGHGPSRAKNPSHSGILPLGYRLEEVDELPTYEYRCHECE
                     HQFEVFQKMSDDPVSECEECGGAVRRMLFPVAIHFKGSGFYTTDYARKKTLSVSSGNG
                     SSDSGGSGGEGASDSDRPNGNGSSATSGAAGSDGAAAKKNADASAKKESVVSSKTGDS
                     "
     gene            complement(5107..5826)
                     /locus_tag="VFD74_04715"
     CDS             complement(5107..5826)
                     /locus_tag="VFD74_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK48884.1"
                     /translation="MGGDKLYRWDVIVPAQRYVFAVEDGTVVEFDRPGDLPHDRFTRY
                     QEIKSEHPVHGMIAWPNTVLLRGPLTVIVDPGMIMQGPPVLLGLERLGVDPGDIDLVV
                     NTHHHVDHTHGNAYFPSITCAIHKWEYNRYLAEYRLGFEPPNVRLLEGEEGELGPDLF
                     FVRTPGHTAASICVVAETAEGTVVVAGDTVGPLPAYFTSMELPAGFPGRDDLLRSWRR
                     IKGLDPAVVIPGHNPPIHLGE"
     gene            complement(5908..6084)
                     /locus_tag="VFD74_04720"
     CDS             complement(5908..6084)
                     /locus_tag="VFD74_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48885.1"
                     /translation="MDWGRIRSFLWGGFFGGLLGVVLAPRGSRKALNPAFPRPEESHL
                     FSGAPCYREDDQFT"
     gene            complement(6102..6416)
                     /locus_tag="VFD74_04725"
     CDS             complement(6102..6416)
                     /locus_tag="VFD74_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48886.1"
                     /translation="MVGFVYYFLLSTVGFFFLAFLAGAGIGYVMGELVLRAGRFYRGV
                     EPAAVAVAGTVWAFVFPPVLSAIVRFGLSPHVVLFGFSRGAVVNWLVMAVAGYFAWQR
                     VR"
     gene            complement(6690..7109)
                     /locus_tag="VFD74_04730"
     CDS             complement(6690..7109)
                     /locus_tag="VFD74_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZK48887.1"
                     /translation="MVSDDKTRPLFMISVAAELADMHPQTLRMYERRGLIRPNRSSKR
                     TRLYSMEDVERLRRIQGLTTMVGLNLVGVEKVLELEGQLEELLRRVEQLESELDAASQ
                     EAQSELEAAHRSYRKELIRVPSREVAVHAAFRRSGSC"
     gene            complement(7131..>7463)
                     /locus_tag="VFD74_04735"
     CDS             complement(7131..>7463)
                     /locus_tag="VFD74_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZK48888.1"
                     /translation="VEESGVFHRRGSDFVIDVPITLAEAALGAMVPVPTPDGKVALKV
                     PAGVRDGTMLRIIGKGSPKLGEGGSGDLLARIAVVTPTDLTEEERGLLEQFATLRGED
                     PRTGLPGW"
BASE COUNT         1319 a         2553 c         2418 g         1173 t
ORIGIN      
        1 tgagggatga cgtcgccggc ccgctgcacg atcacggtat cgcctacgcg caggtccttg
       61 cggcggatgt cgtcttcgtt gtgcagggtc gcttggccaa cagtcacccc gcccacctcg
      121 accggttcca gcaccgcata cgggttgagc atcccggtgc ggccgacgtt gatgcgtatg
      181 tcgaggagcc gggtctgcgc cgtgctcggt gcgaacttgt aggcgaccgc ccatcgcgga
      241 tcccggccgg cggcccccag agccagctgc acggtgcggt cgtcgatctt gaccacgaca
      301 ccgtcgatgt cgtatccgag agaggcccgc cttctctccc agtcacggca gacggccagg
      361 atctcatcga gtccggcgac ccgccgaacg tcggggctca cgcgaaaacc acgggcacga
      421 agccactcga gcacctgcca ctgtccgccc gaccacgtct ccggggcacc ttcgcccagg
      481 gcatccagca gactcgtccc ggcgagaacg acaccgtagg cccacatcga gacaggacgg
      541 gaggctgtga ctcgagggtc cagctgccgc agcgaccccg cggccgcgtt gcgggggttg
      601 gcgaacgtgg actcgtcgtt ggccctgcga ctctcattca gccgctcgaa cgcctcgagc
      661 ggcaggtaga cctcgccgcg cacctccacc aggtccggca ccggcacctc cgtggacgaa
      721 agcccctgcg ccgacagcat caccagaggg atagccgaga tcgtgcggag gttctgggtg
      781 acgtcttcgc ccaccgaccc gtctccccgg gtcgctccgc tcacgaggac accgtgctcg
      841 taccgcagtg aaacggcgag accgtctatc ttgggctcca gggtgaacct gagcgactcc
      901 ggagcgaagc ccgtctcctc ggccaggcgc accacccgcc gataccacgc ctcgagttcc
      961 gcctcgtcct tggcgttcgc gagggacagc atgggctcca ggtggcggat ccgaccgaaa
     1021 gcctccagcg gctccccgcc cacacgctgg gtcggcgaat cgcctgtgag gaggtccggg
     1081 aactgcgtct ccagcccgcg cagttcgcga aggaggtcat cgtaggccgc gtcggcgatg
     1141 atcgggctgt cgagcccata gtagagatgg tcgtggcggc ggatgagcgc gcgcagttcg
     1201 agcacgcgcg ccgcggtctg atcgtacgca ggccccggtc ccgccgtcat gagtctccgg
     1261 tcagcgtgtc ggatccggag agcaccactt caagttgcgc catcgtccgc accatccggt
     1321 ccggcgactc ggcggccagg tcgccctcag ggaagacgcc ccaggcgaca cctaccgagc
     1381 gcagacccgc ggccgtcgcg gcctggagat cgtacggact gtcgcccacg tatacggcgc
     1441 tctcgggagc cgccccgagt gaatcgagcg cgcgcagcag aggttcggcg tggggcttgt
     1501 ggtaggtggt ctcccccgcg cacacgaccg cctcgaacaa ctctcccacg ccggtgacgc
     1561 gaaaggccat ctcggtagtg tgtcgagact tggacgtcac taacgccagg cggaccccgc
     1621 gcgcacgaag gcggagcaac aggtcggcca tgcccgggta gagccgcact cggcggtcgt
     1681 gttcgcgatg gttgtagtcg cggtacgcat cgacgagttc ctgcgcccgc acctcgtcga
     1741 acgactccat ctgctccagg agaggccgcc cgacgttgtg cacggcgtct tctctcgtcg
     1801 accggtagcc gaggacggtc aagatcgcgt gctcgaacga gtcgacgatc agttccaccg
     1861 agtcgagcac ggtcccgtcg agatcgaaca tgaccacccg cggcgcgggc tcgagcggcg
     1921 agaggctcgg gctcgaagag gacgctgcgg tctcgtacgc ggtctcgtca gcggtctcgt
     1981 tcccggtcgc ggtctcggcc gcacgaccca tcgcagcgtc gccgaccgct ggtgcggcat
     2041 cgcccggctc ggcgtcgccg cccgactcgg cgtggcttcc gggaatcccg gcatcgcccg
     2101 gctcggcccg ttcccggcct tcgccgggga acgcccacgc acccgccgcg ggctccgtga
     2161 cggcgtccgc aaggtccagt ctccagtcct ccattcggcg cagcacgtcg tgcgcggtca
     2221 gatggaagtg gatgggggtc acgctgacga aaccgccgcc gaccgcttcg aagtcggtgc
     2281 caacctcatt gtggtagtcg aggtcatcgc cgtagacgaa gtagcggcga cgcgaccccc
     2341 gactctcctg cagttgcacg cggtcgccgt agatgcgctt tccaagcgtg gtcaaccgga
     2401 tccccgacaa cgcttcccag tcgagatcgg ggcagttgat gttcagcagc gcctcggccg
     2461 ggaacccctt ctcgaggatc gactccacca ccagacggga gacccgagcg ggcacactga
     2521 gatcgtagcc gtcgtgatag ccgtcggccg ataccgccag agaggggatg cccagcatga
     2581 tgccctcgaa cgcagcggcg acggttcccg agtaggtgat gtcgtcgccc aggtttccgc
     2641 tcagattgat cccggacacg atgaggtcgg ggcggcgctc caagaagccg agcgccgcca
     2701 aacggacgca gtccaccggc gtgccgtcgg tggagtgtcc catcgatcca tcgggcagga
     2761 tccgctcctc cacccacaga ggggccctca tcgtgatgct gcgcgccgcc ccggtgcggt
     2821 tgcgatctgg ggctatcacg tgcacgtcac cgagggggtc gagagcgcgc ttgacggcga
     2881 agagaccagg cgaatcgatc ccgtcgtcgt tggtgagcag gatcgtcagg cccgagcggg
     2941 gcaggtggac gagagggtgt tcggtgctca tcatggtccg ccaaggatag gtcacggcgc
     3001 gggaacggtc cagtagacgc cggccctgcc gcccacccga agatcgagca gcaggctacg
     3061 gaaggatcag ctccggtctg ctcagtgtgg gaacacaggg agcgacgaac tctccctgca
     3121 catacgagac gcccgcccga cgaagggtgg tgaactcccg cctgtcgatg caatcagcgg
     3181 cgaggatgcg catgtcgtga cgagtggtga accgactgag catgagcagg tactcgaact
     3241 catcctgatg acgatgcacg tcccgcacca gcctgccgct cacccggaca acatcggggc
     3301 tcaactcctt gaggtagtcg agcggcaacg gcctgttggg agagaggtcc acacagagcc
     3361 ggaagcccat gtcccccact gagcgccagg cggacagtcc ggcggggaag tgatcggcca
     3421 acctgtccga acccaccaga aagagcacgt tggccggcgt gatgcgcgca ggttcacggt
     3481 ggtagagcag ggacatcaac cggacggcgc tctcgagaag ctcctcggcc gtagccctca
     3541 gcacgagttg ctcggcgccg tccagcgcat cgtccacggc gctgagcgtc gcatgatggt
     3601 acagatccag gaccctcaac tcctgtcccg tccgttccgc cacgtcgaag acgacgtcgc
     3661 cgtgctgcag gttcgagtag agcggacctt ggaccaccgc gtcgaacgcg acggtcttgc
     3721 cggactcgat ggctgcgatc ggttggaaga gggtggtcag ccggccttcg tcgagcgccg
     3781 accgcaagtg gtcccccatt tccacgagac gcgcggcgtg cttctcccgc gcgctccgct
     3841 ccgcttctac cagcccggct acgagcgcgt cctcgagcgt cccggcggcc tccggacgat
     3901 tcaccaaccc ggcgcccacc tcgatcgtgg tgcgctccat gagctcggcg gcgacgccat
     3961 cggacaggag ttcacgcacg aggtccgcca ctcgatcggc gaccaggtcg acgatctcca
     4021 accccaggtc ggccgactcc cgcgggccgg agagaaggat agcgaagccc tctcccctca
     4081 gacctagatc ggccgggagg tcgagctggc gcaggccatg acccaacata cccatggccg
     4141 cgcccgaggc gcgttcgtag acgctctgga tctcgcgcca gccgtagagt cgagaggtgt
     4201 ggccccaatg ctcgaaccgg acgaacagca ctcccattgt gctcgtcctc tcgagttcgt
     4261 gacgtgcagc ctcgatgacg ctgccaaggg aggggagcag tgacgacggg tcgtcggtca
     4321 ggccgcggag ccaccgaccc agcggcgagg cccacgcaga agtggggtcg ccggcatgtc
     4381 tgcccgggtt cggacttgaa agacttggga cgcgtgcggc agatccgacg gtccgctcac
     4441 cgccgggaga ctcggaagga gaaggcggcc ggtcgttcat cgcgaacccc ccgcccggga
     4501 ctctctagga gtcgccggtc tttgaagaga ctacgctttc cttcttcgcc gacgcatcgg
     4561 cgttcttctt cgcagcagca ccatccgagc ccgccgcacc ggaagtcgcg gaagaaccgt
     4621 tcccattcgg cctgtcgctg tcggaggcgc cctcaccgcc ggatccaccg ctgtcggagg
     4681 agccgttccc gctggaaacg gacagggtct tcttccgggc atagtcggta gtgtagaatc
     4741 cgctcccctt gaagtgtatc gctacgggga agagcatgcg acgaacagcg ccgccgcact
     4801 cctcgcactc ggatacaggg tcgtcggaca tcttctggaa gacttcgaac tggtgctcac
     4861 actcgtgaca ccggtattca tatgtaggca actcgtctac ctcctcaagg cggtaaccga
     4921 ggggaagtat acccgagtgc gacgggtttt tcgcgcggga gggaccgtgt ccgctggcac
     4981 tcctcactgg ggagtgccaa ttgtgtccac cggccggccg atccgcggct catccacacg
     5041 ctctccgctc gtccgtgcgt cccgctctcg cagtcgccgc agccccgcct cgccgccact
     5101 ccccgctcac tcacccaggt ggatgggcgg attgtgaccc gggatgacca cggctgggtc
     5161 aaggcccttg atgcgccgcc agctgcgcag taagtcatcg cgaccaggga agcccgcagg
     5221 gagttccatg cttgtgaagt aggcggggag gggtcccacg gtgtctccgg cgacgaccac
     5281 ggtgccttcc gctgtctccg ccaccacgca gatcgaagca gcggtgtggc ccggagtgcg
     5341 cacgaagaag agatcggggc cgagttcgcc ttcctcgcct tcaagcaggc ggacgttggg
     5401 gggctcgaaa ccgagtcgat actcggcgag atagcggttg tactcccact tgtgtatggc
     5461 gcacgtgatg ctcgggaaat acgcgttgcc atgcgtgtga tcgacgtggt ggtgggtgtt
     5521 aaccaccagg tcgatgtccc ccgggtcgac gcccagtcgc tccaggccga gaaggacggg
     5581 gggtccctgc atgatcatcc cggggtcgac gatcaccgtg agcggcccgc gcaagagcac
     5641 ggtgttgggc caggcgatca tcccgtgcac cggatgctcc gacttgatct cctggtagcg
     5701 ggtgaagcgg tcgtgcggaa ggtcaccggg gcggtcgaac tcgaccaccg tcccgtcctc
     5761 caccgcgaag acgtagcgct gcgccggcac gatgacgtcc cagcggtaca acttatcgcc
     5821 ccccattccc actcacctcc gtagggcgcg ccgtcaggcc gcgcctagag ccatccggga
     5881 cggtcgtgcc cggaccgatt tccgacctca ggtgaactgg tcgtcctcac gatagcaggg
     5941 cgccccggag aacaggtgtg actcttccgg ccgagggaac gcggggttca gggctttgcg
     6001 acttccccga ggcgctagca ccacgccgag tagtccgccg aagaaccccc cccacaagaa
     6061 actcctgatc ctgccccaat ccatctgcgg ctcacctcac cctagcggac gcgctgccat
     6121 gcgaagtacc cggcgactgc catgaccaac caattgacca ccgctccccg ggagaacccg
     6181 aagagcacga cgtgcggact cagaccgaag cgcacgatgg cggagagcac gggtgggaac
     6241 acgaaggccc acacggttcc ggcgacggcg acggccgcgg gctccactcc ccgatagaac
     6301 cgacctgcgc gcagcactag ttcacccatc acatacccga tgcccgcgcc tgcaaggaac
     6361 gccaggaaga agaagccgac ggtcgagaga aggaagtagt agacgaaccc gaccacggcg
     6421 ccgcccgcca gggcggccgc gatggaccgt gcccatcgac cagggccaaa ggtgagacga
     6481 gcggaccgag ggaggcgcgc acactccctg cacttgatcc cgacggccga gtagaccatg
     6541 cagtcggtgc agatgggctt gctgcaggag ctgcagcgaa cgtacgtctc tcgcttgggg
     6601 tgattaacgc acgtaagagg cgctatcgta cccggggtcg cagcgtcccg gtcgggatca
     6661 gcggggggca tgacacagac tccgagggat caacacgagc cggaacggcg gaaggcggcg
     6721 tgcaccgcga cctctcttga cggcacgcgg atgagttcct tccggtagga gcggtgcgcg
     6781 gcctcgagct cgctctgggc ctcctgggac gccgcgtcga gctcggactc gagctgttcc
     6841 acccgccgaa gaagctcctc gagctgacct tcgagttcca gcaccttctc gactcccacg
     6901 aggttgagcc cgaccatggt ggtgagcccc tggatgcgcc gaagacgctc gacgtcctcc
     6961 atcgagtaga gccgtgtgcg tttcgacgag cggttcggcc tgatgagccc gcgccgttca
     7021 tacatgcgaa gagtctgagg atgcatgtcg gccagctcgg cggcgaccga gatcatgaac
     7081 agcgggcgcg tcttgtcatc ggaaaccatg gctcaccccc cgggactcca tcaccaaccg
     7141 ggcaacccgg ttcgcggatc ttcaccccgc aacgtcgcga actgctcgag aagcccacgc
     7201 tcctcttcgg tgagatcggt cggcgtgacc accgcgatcc gtgccaggag gtcgccgctc
     7261 ccaccttccc caagcttcgg cgaacccttg ccgatgatgc gcagcatcgt tccgtcacgg
     7321 acgcctgcgg gcaccttgag agccacttta ccatctgggg taggcaccgg caccatcgct
     7381 ccgagagcgg cctcggcgag cgtgatcggc acgtcgatga cgaagtcgga gccccgccga
     7441 tggaacacgc cgctctcctc gac
//
LOCUS       DASRMJ010000171         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_415623, whole genome shotgun sequence.
ACCESSION   DASRMJ010000171 DASRMJ010000000
VERSION     DASRMJ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415623"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1059
                     /locus_tag="VFD74_04740"
     CDS             169..1059
                     /locus_tag="VFD74_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48889.1"
                     /translation="MEAVITTSGLTKTYGNDRGILDLDLEIEAGEVFGFLGPNGAGKT
                     TTIRTLLNFLRPTSGSATIFGLDSRTESVAIRAAVGNLPGEFTLEEKLTGEQLLRYYG
                     RLRGVHDLSYGHELARLIDADLKRPIGRLSRGNKQKIGLVQAMFHRPPLLVLDEPTGG
                     LDPLVQEAFLDVIRETAAEGRTVFFSSHVLSEVERVCDRVGIIKEGRLVALETTETLL
                     HKRMRRLTATFEVQVDGALFAAIPGVEDVRVDGKSITLAVRDDLDAVVKLVARYPLAA
                     MELEYPNLEEIFLAYYGGGS"
     gene            1056..1928
                     /locus_tag="VFD74_04745"
     CDS             1056..1928
                     /locus_tag="VFD74_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZK48890.1"
                     /translation="MNGGIVSDEAAIGGAAARGGVAIPEWPVRLGALIGHQLRSRMRS
                     TIVWSLSLGLLGTLYVALFPTMKGQLDQYMSALPEEYLAFLGLGTSSMSTLAGFLSIE
                     MHSIIAPLALAFLPIIMGARAIAGAEETKSLDVLLSNPLPRWMIVACAFLTMVVELGI
                     IVLALALLILVPTLMVGESLSVANLFAGSLNLIPFCLFFGGLALLFSAAFRRPGLAIA
                     IPGAVLVASYVLDGLAAAIDAIAGLRPLSIFYHYGSAIETGMPWGSAAVITLFAVAFA
                     GSAMALFYRRDIYT"
BASE COUNT          328 a          703 c          717 g          392 t
ORIGIN      
        1 ccacaaccac atcgcccccc acgaccacca cgactaccac gactacgggg tagggcgtct
       61 cggctacggg gtagggcggg cggatcgaag gttcggtcga gggtcggggc ggcgtctcag
      121 ctgcagcccc gggcgtcgac gcgtcggacg gcgcggaagg gagacagcgt ggaagcggtt
      181 atcacgacca gcggcctcac gaagacctac ggcaacgacc gcggcatcct cgacctcgac
      241 ctcgagatcg aggcgggtga ggtcttcgga ttcctcggtc cgaacggcgc cggcaagacc
      301 acgaccatac gcacactgct gaacttcctg cggccgacct ccggttcggc cacgattttc
      361 ggcctggact cgcgcacgga gtcggtggcc atccgcgccg ccgtgggcaa ccttccgggt
      421 gagttcactc tggaggagaa gctcaccggg gagcagctgc ttcgctacta cggccgcctg
      481 cgaggcgtcc acgatctgag ctacggacac gagttggcgc gcctcatcga cgccgacctc
      541 aagcggccca tcggccgcct gtcccgtggc aacaagcaga agatcggact cgtgcaggcc
      601 atgttccacc gcccgccgtt gctcgtactc gacgagccga cggggggcct cgatccgctg
      661 gtgcaggagg ccttcctcga cgtgatccgg gagaccgcgg cggaggggcg cacggtgttc
      721 ttctcctcgc acgtcctcag cgaggtggag cgcgtgtgcg accgcgtggg catcatcaag
      781 gagggtcgcc tcgtcgcgtt ggagaccacg gagactcttc tgcacaagcg gatgcgccgg
      841 ctcaccgcga ccttcgaggt gcaggtcgac ggcgccctct tcgcggccat ccccggcgtc
      901 gaagatgtga gggtggacgg gaagtcgatc acgctcgccg ttcgcgacga cctggacgcc
      961 gtcgtcaagc tggtggcccg ctaccccttg gccgccatgg agctcgagta ccccaatctg
     1021 gaagagatat tcctcgcgta ctacggaggt gggtcgtgaa cggcgggatc gtgtcggacg
     1081 aagcggccat tggaggcgcc gcggccaggg ggggcgtcgc gatccccgag tggcccgtcc
     1141 gtctcggcgc gctcatcggc caccaactgc gttcgaggat gcggagcacg atcgtgtgga
     1201 gtctgtcgct cggactactg ggaacgctct acgtagccct cttcccaacg atgaagggtc
     1261 agctcgacca gtacatgtcg gctctgcccg aggagtactt ggcctttctg ggcctgggga
     1321 catcgtccat gtcgacgttg gccgggttcc tgtcgatcga gatgcatagc atcatcgctc
     1381 cacttgccct cgcctttctt ccgatcatca tgggtgcgcg cgcgatcgcg ggtgccgagg
     1441 agacgaagtc gctggacgtg cttctgtcga atccgctccc ccggtggatg atcgtggctt
     1501 gcgcctttct gacgatggtg gtggaactgg gcataatcgt cttggcgctg gcgttgttga
     1561 tccttgtgcc gacgctgatg gtgggcgagt ccctgagcgt ggcgaatctg ttcgccggtt
     1621 cgctcaacct catccccttc tgcctcttct tcggcgggtt ggcactcctc ttctcggcgg
     1681 ccttcagacg tccgggcctt gcgatcgcga tcccgggagc ggtgctcgta gcgagctacg
     1741 tgctcgatgg gctggcggcg gccatcgacg ccatcgcggg ccttcggccg ctctccatct
     1801 tctatcacta cggctcggcc atagagacgg gcatgccttg gggcagcgcg gcggtgatca
     1861 cgctgtttgc ggtggccttc gccggctccg ccatggccct cttctaccgc agggacatct
     1921 acacgtagcg ggtgaccccg cgacgcgcct cgccatcatc gggtcccgac ggcgtccggg
     1981 tacggtgagc ggagcgatcc gcgacgactc agtaggcgga atcctccgtc gcgccgccga
     2041 ccctgctccg cgtgaatgtg cggtaaatga gcgccgtgta cgccaggacc accggcactc
     2101 cgatcgcggc gatgacgagc atcgcgctca gggccaggtg
//
LOCUS       DASRMJ010000172         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_455811, whole genome shotgun sequence.
ACCESSION   DASRMJ010000172 DASRMJ010000000
VERSION     DASRMJ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455811"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFD74_04750"
     CDS             <1..555
                     /locus_tag="VFD74_04750"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HZK48891.1"
                     /translation="PDLLVVDEPFAGLDPVNTRLIKEIIEEQASAGCAVIMSTHQMYQ
                     VEALCARIALIDKGRTVLYGDVRDIKRDFAGNALAVRGHGDFENIPGVLEVRRFDGSW
                     RLSLESDADPQQVLRRLVERDVIIESFEVAEPSLDDIFVAVVQGGAGGARAADRTTEN
                     SDGNTSAEHRTVGASTREDGTVGE"
     gene            557..1741
                     /locus_tag="VFD74_04755"
     CDS             557..1741
                     /locus_tag="VFD74_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK48892.1"
                     /translation="MWLVARYEYLRTTRRRSFLLGMLAIPAVLGVIILAGVLVSGTVD
                     PLDSAGYVDGSGILEPPAGRAGESLPRRFADEAAARRALEQGEIQTYYLIPPDYLATR
                     QVSIKFLGDPPSDKLQNDFDGFIRDSITSGLPDEVRTRLRDGFDVTLRTASGRTMNSS
                     GFANLILPGLVGFLFMFAVLTTGGYLMQAVADERENRTVEVLATSLTPEQLIVGKAIG
                     LVAAALTQLAVWSITVTAALVVAARYVEFLARLTPPVSLMVVAMVFFLPSFALVSGVM
                     TLVGSVVGDSRQGQQLSAIINMFFVLPLFFLTIMMESPDGILAVALTLFPTTSMVTVA
                     MRWGVTDVPIWQLGLAFALVSAAAGAAVWAAPRVFRTGMLRYGQRLAPRHLFGGLRSG
                     GR"
     gene            1738..3015
                     /locus_tag="VFD74_04760"
     CDS             1738..3015
                     /locus_tag="VFD74_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK48893.1"
                     /translation="MMAAPRQTTLRTPRSLLVAKYELVSTLRRRSFWVMALLFPAVVI
                     VFSVMSQFVAGRSADRGTGAVGPQSAAVGYVDEASLIKHLPAGVDPSWVTPLSDESAA
                     RRALAAGEIARYYVIPPGAVAGGDLVRVERQFQPLSALDADPLIRYIIVANLFDDQER
                     ALVYQDPTPDPELVSLAGAATGAEAEKQGQPNPLVAYAVAFVMFFVITTTGGFMLQSV
                     SREKESRTAEILLLSISPRQIMSGKLLGLTAVALVQIGFWAGAITLALDQARATLDLV
                     IPRGIPAATYVAGLLFFLLGYLLYSSALGALGALAPTSREAGQFTMVILLPLSAPLFL
                     NAVIMMAPNSAVAVAMSIFPLTAPVAMAARMVIVTVPTWQVVVGLAGLAAATYFFVAL
                     SARFFRADTLLSGASMSLKRVREGFGGRRVGSS"
     gene            complement(3047..>3496)
                     /locus_tag="VFD74_04765"
     CDS             complement(3047..>3496)
                     /locus_tag="VFD74_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HZK48894.1"
                     /translation="ARKVVYFLDNCGEIVFDRLLLEVAGDYGPFAAPPGSRPPEVMVV
                     ARSLPVLNDATVLEALGVGLGEVARVVGNGIEAALPGTVLDQLSPEVRASVAEADLVV
                     SKGGANFELLEEEAALRGKVTFLLLGKCLPLCRTLGVPRDGLIVRNA"
BASE COUNT          518 a         1216 c         1162 g          600 t
ORIGIN      
        1 cccgatctct tggtggtcga cgagcctttc gccgggctgg accccgtcaa cacgcgcctc
       61 atcaaggaga tcatcgagga gcaggcctcg gccggctgcg cggtcatcat gagcacccac
      121 cagatgtacc aggtggaggc cctgtgcgcc cgcatcgccc tcatcgacaa ggggcgtacc
      181 gtgctctacg gcgacgtgcg cgacatcaag cgagacttcg ccggcaacgc gctggcggtg
      241 cgcggccacg gcgactttga gaacataccc ggggtgctgg aggtccggcg gttcgacggc
      301 tcctggcgac tgagcctgga gtcagacgcc gatccgcagc aggtgctgcg ccgactggtg
      361 gagcgtgacg tcatcatcga atcgttcgag gtggccgagc cttcgctcga cgacatcttc
      421 gtggccgtgg tgcagggagg agcgggagga gcccgtgcgg ccgaccggac caccgagaac
      481 tcggacggca acacgagcgc ggagcaccgg actgtgggcg catccacgcg ggaggacggc
      541 acagtcggtg aataggatgt ggctggtcgc ccgctacgag tacctgcgca ccacccgccg
      601 gcgctccttc ctgctgggca tgttggcgat tcccgccgtc ttgggcgtga tcatcctcgc
      661 gggcgtcctg gtgtccggca cggtggaccc actcgattcg gcgggctacg ttgacggcag
      721 tggcatcctc gagccgcccg cgggccgggc cggcgagtcg ctccctcgac gcttcgcgga
      781 cgaagccgcg gcgcggaggg ccctcgagca aggtgagatc caaacctact acctgatacc
      841 ccccgactac ctcgccaccc ggcaggtctc catcaagttc ttaggcgacc ccccgagcga
      901 caaactccag aacgacttcg acggcttcat ccgcgactcg atcacctccg gccttcccga
      961 cgaggtgcga acgcggcttc gcgacggctt cgatgtgacc cttcgcaccg caagcggcag
     1021 gacaatgaac tcctccggct tcgcgaacct catcctcccg gggctggtgg gattcctgtt
     1081 catgttcgcc gtgctcacca ccgggggcta cctgatgcag gcggtcgccg acgaacgcga
     1141 gaaccgcacg gtcgaggtgc tggcgacctc gttgaccccg gagcagctca tcgtgggcaa
     1201 ggccatcgga ctggtcgcgg cggcgctcac gcagttggcg gtgtggagca tcactgtgac
     1261 cgcggccctg gtggtggccg cccgatacgt ggagttcctc gctcgactga cgcctcccgt
     1321 cagcctgatg gtcgtggcta tggtgttctt cttgccgtcg ttcgccctgg tgtctggagt
     1381 tatgacgctg gtgggctcag tcgtgggcga cagccggcag ggtcagcaac tctcggcgat
     1441 catcaacatg ttcttcgtgc tcccgctctt cttcctgact atcatgatgg aatcgccgga
     1501 cggcatcctc gcggtcgcgt tgaccctctt ccccacgacc tcgatggtca cggtggcgat
     1561 gcgctggggc gtcaccgacg tccccatctg gcagctaggc ctcgcgttcg cgctggtgag
     1621 cgccgccgcc ggcgccgcgg tgtgggccgc cccccgggtg ttccgcacgg gcatgctccg
     1681 ctacggccag cgcctcgcgc cgcgccacct gttcggtggg ctgcgctcgg gagggcgatg
     1741 atggcggccc cacgacagac cacactccgc acgccccgca gcctcctcgt ggccaagtat
     1801 gagttggtga gcacgctgag gcgacgctcc ttctgggtga tggccctgct gttccccgcg
     1861 gtggtcatcg tcttcagcgt gatgagtcag ttcgtagcgg gtcggtcggc cgacaggggc
     1921 acgggtgccg tgggtcctca gagcgccgca gtgggctacg tggatgaggc gagcctcatc
     1981 aagcatctac ccgcaggtgt ggacccgtcg tgggtgacgc ctctctccga cgagtcggcc
     2041 gcgaggcgag ccctggcagc cggcgagatc gcccgctact acgtgatccc gcctggtgcc
     2101 gtcgccggcg gcgacctggt gcgtgtggag cggcagttcc agccgctcag cgcactcgac
     2161 gccgaccccc tcataaggta catcatcgtc gccaacctct tcgacgatca ggagcgggcg
     2221 ctcgtctacc aagaccccac gccggacccg gaattggtgt cgttggccgg agccgccacc
     2281 ggcgcggagg ccgagaagca ggggcaaccc aatccgctgg tagcctacgc ggtcgccttc
     2341 gtgatgttct tcgtcatcac cacgaccggc ggcttcatgc tgcagagtgt ctcccgagag
     2401 aaggagagcc gcactgccga gatcctatta ctgagcatct cgccgcgcca gatcatgtcc
     2461 gggaagctcc tgggcctgac ggcggtggcc ctcgtccaga taggcttctg ggcgggcgcg
     2521 atcaccctgg cgctcgatca agctcgggcg acccttgacc tggtgatacc ccgagggatc
     2581 ccggccgcga cctacgtcgc gggcctgctc ttcttcctgc tcggatacct gttgtactcc
     2641 tccgccctgg gggcgctggg agcgctggca cccacgtcgc gggaggcggg gcagttcacc
     2701 atggtgatcc tgcttccgct ctctgccccc ctgttcctga acgccgtgat catgatggca
     2761 ccgaacagcg ccgtggccgt ggccatgagc atcttcccgc tcaccgcccc ggtggcgatg
     2821 gcggccagga tggtcatcgt caccgttccc acgtggcagg tcgtggtggg actcgccggg
     2881 ctggcggcgg ccacctactt cttcgtggcc ctgtccgcgc ggttcttccg agccgatacg
     2941 ctgctctccg gcgcgtcgat gagcctgaaa agagtgcgcg aaggcttcgg ggggcggcgg
     3001 gtcggctcgt cgtagccggc gccgcggcct cggccagcgc ccgacttcag gcgttgcgga
     3061 cgatcagccc gtcgcgcggg acaccgaggg tgcggcagag cgggaggcac ttgcccagca
     3121 gcaggaaggt gaccttcccc cgcagcgcgg cctcctcctc gagcagctcg aagttggcgc
     3181 cgcccttcga taccaccaga tccgcctcgg ccaccgacgc cctcacctcg ggcgacagct
     3241 ggtcgaggac ggtgcccggc agcgccgcct cgatgccgtt gcccacgacc cgtgccacct
     3301 cgccaagtcc cacgcccagc gcctccaaga cagtggcgtc gttcagcacg ggcagcgacc
     3361 gcgcgacgac catcacctcg ggcggccgtg agccgggcgg cgcggcgaac ggtccatagt
     3421 ccccggcgac ctccagcagt agcctgtcga agacgatctc gccgcagttg tcgaggaagt
     3481 agacgacctt ccgggc
//
LOCUS       DASRMJ010000173         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_486829, whole genome shotgun sequence.
ACCESSION   DASRMJ010000173 DASRMJ010000000
VERSION     DASRMJ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486829"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            513..1781
                     /gene="kynU"
                     /locus_tag="VFD74_04770"
     CDS             513..1781
                     /gene="kynU"
                     /locus_tag="VFD74_04770"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HZK48895.1"
                     /translation="MNREERALALDAQDELADFREHFYLPPDTIYMDGNSLGLPSREA
                     EAALLQVLDEWKRLAIGGFLQGETPWISLGERLGELQAPLVGAHPDEVVATGSTTVNL
                     HQLVATFYQPEGRRRKVLADELNFPSDLYALQSQVRLHGLEPAQDLVLVRSRDGRTLQ
                     EDDIVAAMTDEVATVVLPSVLYRSGQLLDLERLTAEAHARGCLIGFDLCHSAGAVPHA
                     LSAWGVDFAYWCTYKYLNSGPGGIAGLYVNRRHHGRLPGLCGWWGYRSDARFAMRPEF
                     EAAPTAAAWQIGTPTMLSAAPLLGSLALFAEAGMERLRARSLALTEYLMELVDTLPED
                     LGYRIGTPREPSRRGGHVAVEHPDAWRVCQALLARGVVPDFRPPDVVRLAPVALYTTY
                     HEVWRAVRHLAAVVQEGDLEHFSAKRGLVS"
     gene            1922..2152
                     /locus_tag="VFD74_04775"
     CDS             1922..2152
                     /locus_tag="VFD74_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HZK48896.1"
                     /translation="MVRTQPNDPLHGVTLETIVRRLVDEYGWGGLAAQIDINCFKSDP
                     SVSSTLKFLRKTPWARKQVEDLFVSSKLWTRD"
     gene            complement(2347..2550)
                     /locus_tag="VFD74_04780"
     CDS             complement(2347..2550)
                     /locus_tag="VFD74_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48897.1"
                     /translation="MRPRLGYQGVSPRVLFMSGYIRNAIVPDGRLDPGVDFLEKPFTP
                     AALLRAVREVLDRPREGQLGLPI"
BASE COUNT          433 a          936 c          884 g          395 t
ORIGIN      
        1 cccctcgacc gtggccgtgg tatcgggtgt ctcggtcgag gtcgtcacgc cgagcgagcc
       61 cagctcctcc tccgatggcc cgcaaccgca cccgggcagc aacacgacga gagacaacac
      121 cgcagccaga aggacgaggg ccacggccgg ggtcccgatc gcgtcaacga tcctccgctt
      181 ctgttccccg acccgccgag ctcttctcca gccaagaccg ctgccgcttg ccaccaagtg
      241 ccccctccgt cgaccccgcc caagaagccg caaggttgcc ccatgcgtgc ggcaaggtca
      301 agcaagcgac ctgtgcctcc ctgacggccc cctcaacggc gatccccgcc gagctgagct
      361 gggcgttgaa cagccgcgtc acatgctcat tgaccgcagg cacccgtgcg cctaccattg
      421 cccgggcgga tgtgagccga cacggatgag ccacgacggg aggtccgact gatggtgcgg
      481 ctggagacga tcgagtacgc ggcagccccg cgatgaaccg cgaggagcgc gcgctcgcac
      541 tggacgcgca ggacgagctc gcggacttca gggagcactt ctacctcccc ccggacacca
      601 tttacatgga cggcaactcc ctcggtctcc cctcccgcga ggccgaagca gcgctgctgc
      661 aggtgctgga cgagtggaag cgtctggcca tcgggggatt cctccagggg gagacgccct
      721 ggatctccct gggagagcgc ttgggcgagc tgcaggctcc gctcgtgggc gcccatcccg
      781 acgaggtggt tgccaccgga tcaacgaccg tcaacctgca ccagctcgtg gccaccttct
      841 accagcccga aggacggcgc cgcaaggtac tggccgacga actcaacttc ccctccgacc
      901 tctacgcctt gcagagccag gtgcggctcc acggcctgga accggcccaa gacctggtgc
      961 tggtgaggag tcgggacgga cgcacgttgc aggaggatga catcgtggcc gccatgaccg
     1021 acgaggtcgc aacggtggtg ctgccctccg tcctctaccg cagcggccag ctgctcgacc
     1081 tggaacgcct caccgcggag gcccacgccc gagggtgcct catcggcttc gacctctgcc
     1141 actcggcagg agcggtgccg cacgcgctct cggcgtgggg tgtggacttc gcgtactggt
     1201 gcacgtacaa gtacctgaac agtgggccgg gcgggatcgc cggcctgtac gtgaaccgcc
     1261 ggcaccacgg acgactcccg gggctgtgcg gatggtgggg ctaccgcagc gatgcgcgct
     1321 tcgccatgcg accggagttc gaagccgcgc cgacggcggc ggcctggcag atcggcaccc
     1381 ccaccatgct gagcgccgct cccctgttgg ggtcgctggc gctgttcgcc gaggcaggca
     1441 tggagcgtct gcgcgcccgc tcgctcgcgc tcaccgagta cctcatggag ttggtagaca
     1501 cacttcccga ggacctgggc taccgcatcg gcacgccgcg agagccgtcg cggcggggtg
     1561 gccacgtggc cgtggagcat ccggacgcct ggcgcgtgtg tcaagccctg ctcgcgcgag
     1621 gggtggtgcc cgacttccgg ccgcccgacg tggtccggct cgcgccggtg gccctgtata
     1681 ccacgtacca cgaggtgtgg cgcgcggttc gccacctggc ggccgtggtg caggaagggg
     1741 atctggagca cttcagcgcg aagcgcgggc tggtgtcctg agggcgtgcg tccccggggc
     1801 tctggcgggt gttccgaggg cgtgcgtccc cggggcgcgg cctatccctg ccgggatggg
     1861 gagtacaatc ggcggcgtag cgcccacgaa tgccaacctt ggcgcaattg agcgagaggg
     1921 gctggtccgc acgcaaccca acgacccact tcacggcgtg acccttgaga cgatcgttcg
     1981 caggctggtc gatgagtacg gctggggcgg gctcgccgcg cagatcgaca tcaactgctt
     2041 caagagcgat ccgagcgtca gttcgacgct gaagttcctg cgcaagacac cctgggctcg
     2101 caagcaggtc gaggacttgt tcgtgagctc caagctctgg acacgcgact agctgaaagc
     2161 ccagcctcgt cgaggccgcc gttgactctg gcgacaaccc tggcgtcaac cctggcggcg
     2221 gcgacgccga ccggggcgct cagctccagc tcggctcgcc cgcccgccga ggcgtccgaa
     2281 gcgggcaagc tcccacggat acgggatccg acggcaacct ccgctcccca cccactcccg
     2341 ccttcctcag atcggaagac cgagctgccc ctcgcgcggc ctgtccagca cctcgcgcac
     2401 cgcgcgcaga agcgccgccg gggtgaaggg cttctcgaga aagtcgacgc ctgggtccag
     2461 acgaccgtcg ggcacaatgg cattccggat gtagcccgac atgaagagca cgcggggcga
     2521 gacgccctga tagccgaggc gtggtctcac ttccaccggg cgaggaggaa cggggtagtc
     2581 gccagttcag cccctacctc ggattcatcg cgatcatggt cggcttcctc atcgagtggc
     2641 ccacacct
//
LOCUS       DASRMJ010000174         5962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_302557, whole genome shotgun sequence.
ACCESSION   DASRMJ010000174 DASRMJ010000000
VERSION     DASRMJ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302557"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFD74_04785"
     CDS             complement(<1..836)
                     /locus_tag="VFD74_04785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZK48898.1"
                     /translation="MDFRYSTEQELFRQGVREWATKNLEPRARQIDKDENGIPDDIVQ
                     GMVDLGIFGVTVPEELGGCAVPGEELIYAMIAIHEIARADLSMSTPVYTLLTIGWSYI
                     VNKFGTQQLREEVLPAAAAGKAFVGINTTEPGGGSDLARIKTRADKKDGAYVLNGEKA
                     YVSGPKECMKLGAPSGHCSLVVTDPTTGHKGLTFIYIPAHLEGISWTVYEDMGRMGLS
                     TGGWLYKDAEIPAYCLLGEEGKGFYTVMEGFNAARVLVAAACLGGAEKALEISAEYTQ
                     QR"
     gene            complement(1041..2228)
                     /locus_tag="VFD74_04790"
     CDS             complement(1041..2228)
                     /locus_tag="VFD74_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZK48899.1"
                     /translation="MAGALEGLKVLDLSRVVAGPMCAMMLADLGADVIKVERPIAGDD
                     TRTWGPPYVGTESAYYLCVNRNKRSITVDIKSEAGRDLVRRLARGSDVLVENYKTGNL
                     DRLGLGYESLREENPGLIYCSITGYGHTGPDAYQPGYDFVIQGRGGIMSITGEADGPP
                     MKVGLAIVDVTAAMNATIAVLAAVEARRRTGLGQMIDIALLDSHVAWLINHASNYLVA
                     GEVSKRRGNSAPNITPYGSYKASDGWFNIAVGNDSQFRRLCELIGAPELSTDPRFATN
                     PGRVENDAQLVAILGEHFAQRSTAEWLDLFYKGNIPAGPVKSIPEVMEDPQVLAREMI
                     VELPHPTAGSVRLVGSPLKMSETPVTYRSPPPLLGEHTDQILVEFGYSEEQIAALRAE
                     GVV"
     gene            complement(2476..2898)
                     /locus_tag="VFD74_04795"
     CDS             complement(2476..2898)
                     /locus_tag="VFD74_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HZK48900.1"
                     /translation="MVIPVAFKRNGDRLTCVDEHGQSDMPFTYLFSGYARLPQNVSHQ
                     ALYERVSVVLEVDQRGIIVECSSTLVTALAGDFLNRLLKGRSVLDGRAATEDLIRARY
                     RGHSQGALIYALRKALEAVDASPLVPDVQAAELPASEH"
     gene            3010..4518
                     /locus_tag="VFD74_04800"
     CDS             3010..4518
                     /locus_tag="VFD74_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HZK48901.1"
                     /translation="MAATHEDDPYDLIAPRRVTDEVVRQLRALISQGELGAGARLPSE
                     RRLAELLGVGRPAVREALRELRVQGLVQTGRGRQGTSVAAPALDDGLTTAQQVAQLLE
                     LRMAVETQAAALAVRRADAADLDALQASLPEGDNLDHDDDRRFHDTLAAVSHNPLLQR
                     ALDDLVKRVYDRAASDFPSLYEQPHRGAIRSQHVAIVAAIRGGDEDAARRSVALHLGY
                     VGRILNQLLGAKRDIRMIVCDLDGTLLAAPRLVTERTRAAIASARDEQIAVFLASARP
                     PRSMRAYHALLGLSTPVIAGNGALLWDLESSIPLARESIDHGLAAEIIELGRSLGAIV
                     NAESDDEWFAEGTNERILRNLRTYGLEPPHRVGPLDHLLGGDEPIDKVFLDIRDLAPD
                     VREAARASVRAAFGSRTNLTETAEGLLDLVSLRASKAIMAQRVARSRRIRADQVAAIG
                     DHENDAALLRWAGTGIAMGNATAGAKAAADLVTASNLRDGVAEAIETWILAG"
     gene            complement(4576..4803)
                     /locus_tag="VFD74_04805"
     CDS             complement(4576..4803)
                     /locus_tag="VFD74_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48902.1"
                     /translation="MESVSEGVENLSGDEVRSRIAELEEELEDLAMERSMTLGGTGVH
                     ISAGEAERLRNEFERDEARVSARLAELRSLS"
     gene            complement(4983..>5962)
                     /locus_tag="VFD74_04810"
     CDS             complement(4983..>5962)
                     /locus_tag="VFD74_04810"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZK48903.1"
                     /translation="APNSQRAAQASCRTPPLTPSAHSASAAAFYMTLGLLAASWVRRW
                     ETRVYVLLAAVFVVAMVGFSRLYLGVHYLSDVLAGYALGAFWATLAITAGMVLDRSLP
                     SRSSTPEAHGMTDHSHPNDGEVAPTPSGDSATGHPHSGGKVFSPKRAGVLDDERRLLH
                     LSEDDLRRLLALRGDEDVADLGSGTGFYANRVAGWTSGSVYAVELQAEMQDLHRRKGA
                     PANVELVLADADQLPLPVGSIDRALSVNTFHEAHGAEGLRRLAAALRPGGLFVIVDWR
                     RAGDALEQGPPLEHRLSTDEVRSLLEPWFEVVSEDVVSTPFFAVVARKR"
BASE COUNT         1029 a         2000 c         1947 g          986 t
ORIGIN      
        1 tggcgctgct gggtgtattc ggcactgatc tccagggcct tctcggcacc gccgaggcag
       61 gcggcagcca ccaatacccg agcggcgttg aagccctcca tgacggtata gaagcccttg
      121 ccttcttcgc cgagcaggca atacgcgggg atctccgcgt ctttgtaaag ccagccaccg
      181 gtggacagac ccatccggcc catgtcctcg tagacggtcc agctgatgcc ttcgaggtgg
      241 gcggggatgt agatgaaggt gaggcccttg tggccggtgg tggggtcggt cacgacgagc
      301 gagcagtgac cgctgggagc gccgagcttc atgcactctt tcggacccga cacgtaggcc
      361 ttctcgccgt tcaagacgta ggcgccgtcc ttcttgtccg cgcgggtctt gatgcgggcc
      421 aggtcggagc cgccacctgg ctcagtggtg ttgatgccca caaaggcctt gccggcggcc
      481 gcggcgggga gcacttcctc gcgcagttgc tgggtgccga acttgttgac gatgtacgac
      541 cagccgatgg tgagcagcgt gtacaccggg gtggacatgg agagatcggc gcgggcgatc
      601 tcgtggatcg cgatcatcgc gtagatcagc tcttcgccgg ggacggcgca gccgccgagt
      661 tcctcgggga cggtcacgcc gaagatcccc aggtcgacca tgccctgcac gatatcgtcc
      721 gggatgccgt tctcgtcctt gtcgatctgc cgcgcccgcg gctcgaggtt cttggtggcc
      781 cactcgcgca caccctgcct gaacagttcc tgctccgtgc tatacctgaa atccatgcga
      841 ctcctccttg acgaccactc tcttccgagc cgatccgcat cgccgaacga cggtgggccg
      901 acccagtcta cacaccgagc gccccaacgg cgccactccc cttcttcctt tcgcctgcct
      961 ccttggtcga accgtcgccg ccaacgaact atctccgccg aacccgggag cggtacagag
     1021 cacccgggag gcgatgatga ctagaccaca ccctccgcac gcagggccgc gatctgctct
     1081 tcgctgtacc caaactcaac aagaatctga tcggtgtgct ccccgagaag cggcggaggc
     1141 gaacggtagg tgaccggcgt ctcgctcatc ttcagcgggc tccccacgag gcggacggaa
     1201 cccgcggtgg gatgcggcag ctcaacgatc atctcccggg ccagcacctg cggatcctcc
     1261 atgacctcag ggatcgactt gacgggaccg gccggtatgt tccctttgta gaacaagtca
     1321 agccactcgg ccgtggaccg ctgtgcgaaa tgttcgccta ggatcgccac cagctgtgcg
     1381 tcgttctcca cgcggcccgg gttggtagcg aaccgtgggt cggtggacaa ctcgggcgcg
     1441 ccgatcaact cgcagagacg ccggaattgc gagtcgttgc ccaccgcgat gttgaaccac
     1501 ccgtcggaag ccttgtacga cccataggga gtgatgttcg gggctgagtt cccgcgacgt
     1561 ttcgacactt cgcctgccac caggtagttg ctggcatggt tgatgagcca cgccacgtgg
     1621 gagtcgagga gcgcgatatc gatcatctga ccgaggccgg tgcgcctcct ggcctccacg
     1681 gccgcgagca ccgctatggt cgcattcatg gccgccgtca cgtcgacgat ggcgaggccc
     1741 accttcatgg gagggccgtc cgcctcgccg gtgatgctca tgatgccccc gcggccctgg
     1801 atgacgaaat cgtatccggg ctggtaggcg tcaggtccgg tgtggccgta cccggtgatt
     1861 gagcagtaga tcagccccgg gttctcctcg cgaagcgact cgtagccgag ccccaggcgg
     1921 tcgaggttgc cggtcttgta gttctccacc aggacgtcgg atccgcgcgc aagccgccgc
     1981 accagatcgc gccccgcctc gctcttgata tcgacggtga tgctgcgctt gttgcggttc
     2041 acgcacaggt agtaggcgga ttcggtgccg acgtagggag gaccccacgt ccgagtatcg
     2101 tcacccgcga tcggtcgctc caccttgatg acgtcggcgc ccagatcggc cagcatcatg
     2161 gcacacatgg ggccggccac gacccgcgag agatccagaa ccttgagccc ttccagtgct
     2221 cccgccactt tccgtcctcc agcgcgagtg atcgacgttg acggcggctt cccgtccgtg
     2281 acgcacagac cggacgcgac gacgccgcac gcggctgtgt ccggcgtgag accgtgcgca
     2341 gccgcatcca ttgcgctggg agctcatggc cgttagtagg gggcagagac ggtgccgacc
     2401 ctgggccatg cgagcagcgg taccagttca tcatcccact ttgtgatgcg gcgttcaagc
     2461 gtccgagagg atgggctagt gctcagatgc ggggagttcc gccgcctgaa cgtcgggcac
     2521 gagcggcgaa gcgtcgacgg cctccagggc cttgcgcaga gcgtagatca gcgccccctg
     2581 cgaatggccg cggtaccggg cgcggatgag atcttccgtg gcggctcggc cgtcgaggac
     2641 cgaacggccc tttagcaggc ggttgagaaa gtcacctgcg agtgcggtga caagggtgga
     2701 agaacactca acgatgatcc cccgctggtc cacctccagc acgacgctga cgcgctcata
     2761 cagggcctgg tgagacacgt tctgcgggag tcgcgcgtac cccgagaaga ggtacgtgaa
     2821 gggcatgtcg ctctggccgt gttcgtccac gcaggtcagt ctatcaccgt tgcgtttgaa
     2881 cgctactggt attaccatac tacctaaccg gcctcggagg cgcgcctcgt tgtcagtccg
     2941 tgcacgacga cccggcgacc acaggcggag gtgtcggggc ccggagatac cgacagtgag
     3001 gtgatctgct tggcagccac acacgaagac gacccatacg acctcatcgc tcctcggcgc
     3061 gtgaccgacg aggtggtgcg acaactgcgc gcactgatct cccaggggga actcggcgcg
     3121 ggggcgcgac taccgtccga gcggcgcctg gccgaactac tgggggtggg tcggcccgcc
     3181 gtgagggagg cgctgagaga actgagggtc cagggactcg tgcagaccgg ccgaggccgg
     3241 cagggcacaa gcgtggcggc gccggccctc gacgacggcc tcaccactgc ccagcaggtg
     3301 gcccagctcc tcgaacttcg gatggccgtc gagacgcagg cggccgcgct cgcggtgaga
     3361 cgtgccgatg ccgccgacct ggatgcactt caggcctcac ttcctgaagg cgacaacctc
     3421 gatcacgacg acgaccgtcg gttccacgac accctcgcgg ccgtgtccca caacccgctg
     3481 ctgcagcgcg cgctcgatga tctcgtgaaa cgcgtgtacg atcgagcggc ctccgacttc
     3541 ccatctctct acgagcaacc gcaccggggc gcgatacgga gccagcatgt agcgatcgtg
     3601 gcggcgatac gcggcgggga tgaagacgcg gcccggcgat ccgtggcgct gcacctcggc
     3661 tatgtgggcc gcatcctcaa tcagctcctg ggggcgaaga gagatatccg catgatcgtg
     3721 tgcgatctgg acggcaccct gctcgcggcg ccgcgcctcg tcacggagcg cacccgcgcc
     3781 gccatcgcct cggcacggga cgagcagata gctgtattcc tcgcctcagc ccgacctccc
     3841 cgctcgatga gggcctacca cgctctgctc gggctttcca cacccgtcat agcgggcaac
     3901 ggcgcgctgc tgtgggatct cgaatcgtcg atcccgctgg cccgcgaatc catagaccac
     3961 gggttggcgg cggagatcat agagctgggc cggtccctcg gcgcgatcgt caacgctgag
     4021 agtgacgacg agtggttcgc cgagggcacc aacgagcgca tcctgcggaa tctgagaacg
     4081 tacgggctgg aaccgccaca ccgcgtcgga ccgctcgacc acctcctggg aggggacgag
     4141 ccgatcgaca aggtcttcct cgacatccgt gacctcgccc ctgacgtccg cgaagcggcg
     4201 cgcgcgtcgg tccgcgcagc tttcggcagc cgcacaaacc tgaccgagac agcggagggg
     4261 ttgctggact tggtctcgct cagagcctcc aaggccatca tggcccaacg cgtggcgcgc
     4321 tcgcggcgaa tacgggccga ccaggtggcg gccatcggcg accacgagaa cgacgcggcg
     4381 ctgctgcgct gggcgggaac cgggatcgcg atgggcaacg ccaccgccgg cgccaaggcg
     4441 gcagcggacc tcgtgaccgc ctccaacctg cgcgatgggg tggcggaagc catcgagacg
     4501 tggatactgg ccgggtaacg agtcggccgc tcggatcaat ccggaagccg tcaggaagtc
     4561 gtccggaaag gggagctaag agagggaccg caactcggcg aggcgggcgg agacgcgcgc
     4621 ctcgtcacgc tcgaactcat tgcgtaggcg ctcagcctct ccggccgaga tatggacccc
     4681 cgtgccgccc agcgtcatgc tgcgttccat ggcaaggtcc tcgagttcct cctcgagttc
     4741 tgcaatcctg gaccgcactt cgtcaccgga taggttctcg accccttcgc tcaccgactc
     4801 catcgaacat cctcctgtcg tgccgccctc gcaagcgcgg ccttgcgtcg ctcctatcgg
     4861 tcagcttata ccccccatgg gtataaaggc aaggaccgag gggcgccgta tcgtccggaa
     4921 ttcgcccggg cctcaggcgc gggcggcgcg ggcggcgcga gcgaggcggg cgggaggcgg
     4981 cctcacctct tgcgcgccac cacggcaaag aagggcgtgc tcacgacgtc ctcggagacc
     5041 acctcgaacc acggctcgag gagcgacctg acctcatcgg tggagagacg atgttcaagc
     5101 ggcgggccct gttcgagggc atcacccgct cggcgccagt cgactatcac gaacaacccc
     5161 cccggtcgca gcgccgccgc aagacgccgc aggccctccg caccgtgagc ctcatggaag
     5221 gtgttgacgc tcagcgctcg gtctatcgaa ccaaccggta ggggcagttg gtcggcatcg
     5281 gcgagcacta gctccacgtt ggcgggagca cccttgcgcc tgtgcaggtc ctgcatctcg
     5341 gcctgtagtt ccaccgcgta gacgcttccg ctggtccacc cggctaccct gttcgcgtag
     5401 aagccggtgc cactgccaag gtccgccacg tcctcgtccc cacggagggc caggaggcgg
     5461 cgcagatcgt cctcactcag gtggagcaac cgtcgttcat cgtcgagcac cccggctcgt
     5521 ttcggcgaga acaccttccc gccggagtgg gggtggcccg tcgcgctgtc acccgaagga
     5581 gtaggagcta cttcaccgtc gttcggatga gaatggtcgg tcatgccgtg ggcctccggg
     5641 gtcgatgagc gggatgggag tgaacgatca agcaccatgc ccgccgtgat cgccagggtc
     5701 gcccagaagg cgccgagcgc gtagccggcg agaacatcgc tcaggtagtg tacgcccagg
     5761 tagagacggc tgaatcccac catcgccacg acgaagacag ccgcgagaag cacgtacacc
     5821 cgcgtctccc agcggcgcac ccaactcgca gcgagcagcc caagcgtcat gtagaacgcc
     5881 gccgctgacg ccgagtgggc actcggcgtc agcggcggcg ttctacatga cgcttgggct
     5941 gctcgctgcg agttgggtgc gc
//
LOCUS       DASRMJ010000175         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_455841, whole genome shotgun sequence.
ACCESSION   DASRMJ010000175 DASRMJ010000000
VERSION     DASRMJ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455841"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFD74_04815"
     CDS             <1..396
                     /locus_tag="VFD74_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HZK48904.1"
                     /translation="ADLFVRELTLVAESLAVPALVISLLLTPVATELPEKFNSVVWMR
                     QGKDTLALGNLTGAMVFQSAIPVAFGIVLTPWRLETEGLLAAGLALVSGLLLWSRVRL
                     TGGLGVGSLLFGGVFYAAFVVVTLGGFLR"
     gene            554..1333
                     /locus_tag="VFD74_04820"
     CDS             554..1333
                     /locus_tag="VFD74_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZK48905.1"
                     /translation="MQIVVCVKQVVDTEAEKKLLPPAWSLDRVSADNVLNPYDEYAVE
                     EAVRLKEAHGGEVTVLCVGPEAAQDAVRKALAMGADQAVMVTDPALAGSDTQGTARVL
                     AAALSDTTYDLVIMGIMSTDAQTGQVPSALGDALDIPVFTALNKIEVDGATIKVHSQT
                     DQGYVEYASPLPAVCSVVKGINEPRYPSLKGIMGAKKKPLDIKDVAALGIEPARVGAA
                     GALTRVVAASSPPPRKAGAKFEDEGTGAARIVDFLVAEKLI"
     gene            1356..2354
                     /locus_tag="VFD74_04825"
     CDS             1356..2354
                     /locus_tag="VFD74_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZK48906.1"
                     /translation="MSNDMRAGLLVVAEQRDGKLASVTLESLTAARGLLADFGGPLAV
                     AVVSGSHADELVAEAARYGAEKAFVAESPELEVFRSGPYTDAAMAAIVAAAPKAVLFA
                     NTAGGRDIASRCARRLGVGLLGDVTRVYVDGGALRVSHPCFGGTVVTEKEAVAEPALM
                     TVRTNSFDKAEAPVQVETHRLTLDFTPSGLLARVIQVVVEDAGMASLEEAQIIVSGGR
                     GLGGPENFAMIHELADALGAAVGASRAAVDAGWKPHQYQVGQTGKIVSPKVYIACGIS
                     GAIQHKAGMQSSDYIVAINKDPDAPIFGFADLGVVGDVFEVVPALTQEIRKRKSAA"
     gene            2401..>3192
                     /locus_tag="VFD74_04830"
     CDS             2401..>3192
                     /locus_tag="VFD74_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HZK48907.1"
                     /translation="MQTRELLFNVPLWAEVAMYLGAAVAGLMLVTDLARRVGMYRRGA
                     AAGARADHPLRRVGDLLVNGILQRDLLRDRYAGLMHLGIFFGFLVLLIGTTILIFQID
                     LGLDFFHGSFYLVFSFVMEIAGLALLGGVLLAAFRRYVRRPARLSRSWDDHYALIVLF
                     VLGVTGFLLEGARIYADGFPSFERGASFVGYGVGKVFSVFAGRDTFVTLHEWGWVVHM
                     ALAVGFVGLLSYTKFLHSLTSPINILLRERRARGSMTFVPDVEERG"
BASE COUNT          495 a         1025 c         1102 g          570 t
ORIGIN      
        1 gccgatctct tcgtccggga gctcaccctg gtcgcggagt ctctcgcagt gccggcgctc
       61 gtaatctcgc tgctgctgac ccccgtggcg acggaactgc cggagaagtt caacagtgtc
      121 gtctggatgc gacaaggcaa ggacaccctg gccttgggca acctcaccgg ggccatggtc
      181 ttccagagcg ctatccccgt ggctttcggg atcgtcctca ccccctggcg actggagacc
      241 gaggggttgc tggcggcagg ccttgcactg gtgtccggcc tgctgctctg gtcgcgggtg
      301 cgcctgacgg gcggtctggg tgtcggttcc ctgttgttcg gcggcgtctt ctacgccgcc
      361 ttcgtcgtgg tgactctggg aggattcctg aggtgagccc acggtggaga ggccggggag
      421 gtcggcgggg gcttcgggcc ccagcggtac cgccgccctc ttcagtagtc ttggcgagag
      481 agtgtttgta gaatagggaa gtcggaggcg ccaccggtgc ctttcgtgag gttcgagcga
      541 aggaggtccg atcttgcaga tcgtggtttg cgtcaaacag gtggtcgata ccgaggcaga
      601 gaagaagctg ctgccacctg cgtggtccct cgaccgggtg agcgccgaca acgtgctcaa
      661 cccctatgac gagtacgccg tcgaggaagc cgtgcgcctc aaagaggccc acggcggaga
      721 ggtgacggtc ctctgcgtgg gccccgaggc cgcccaggac gcggtgcgca aagcactcgc
      781 catgggcgcc gaccaggccg tcatggtcac ggacccggct ctcgccggct ccgacaccca
      841 aggcaccgcg cgcgtgctcg cggcggctct atccgacacg acgtacgacc tggtgatcat
      901 gggcatcatg tccaccgacg cccagaccgg tcaagtaccc tcagccctgg gcgacgccct
      961 cgacatcccc gtcttcacgg cgctcaacaa gatcgaggtt gatggcgcca ccatcaaggt
     1021 gcacagtcag accgaccagg ggtatgtgga gtacgcctcc ccgctgcccg cggtgtgctc
     1081 ggtggtcaag ggcatcaacg aaccgaggta cccgtcgctc aagggcatca tgggagcgaa
     1141 gaagaaaccc ttggatatca aggacgtcgc cgcactgggc atcgaaccgg ctcgtgtggg
     1201 cgccgccggg gcgctcacac gtgtggtcgc ggcttcgtcg ccgcccccgc gcaaggccgg
     1261 cgccaagttc gaagacgagg gcaccggtgc cgcgcgcatc gtggacttcc tcgtcgccga
     1321 gaagttgatc tgacaccacg aaaggaggga acgagttgag taacgacatg cgcgccggcc
     1381 tcctggtggt ggccgagcag agagatggga agttggcttc cgtgaccctg gagagcctga
     1441 ccgctgcacg cggtctgctg gctgacttcg ggggtccatt ggcggtggcc gtcgtgagcg
     1501 gatcacacgc cgacgaactg gtcgctgagg ccgcccgcta cggagccgag aaggcctttg
     1561 tggccgagag tcccgagctg gaggtgttcc ggtccggtcc gtacaccgac gcagccatgg
     1621 cggccatcgt cgctgcggcg cccaaggccg tcctgttcgc gaacaccgcc ggcggccggg
     1681 acatagcatc acggtgcgcg cggcgcctag gcgtgggcct gcttggcgac gtgacccgag
     1741 tgtacgtgga tggcggcgca cttcgcgtga gccacccctg tttcggcggc acggtcgtca
     1801 ctgagaagga ggccgtcgcc gagcccgcgt tgatgaccgt gcgtacgaac tccttcgaca
     1861 aggccgaagc gcccgtgcag gtggagacgc acaggctgac actcgacttc acgccgtccg
     1921 gcctgctcgc gcgggtgatc caggtcgtcg tggaggacgc cggtatggcg agcctcgaag
     1981 aggcccagat catcgtgtcg ggcgggcgcg gcctcggcgg tcccgagaac ttcgcgatga
     2041 tccacgagct cgccgacgca ttgggcgcgg ccgtgggtgc gagccgcgcc gccgtagacg
     2101 ccgggtggaa gcctcatcag taccaggtgg gccagaccgg caagatcgtg tcgcccaagg
     2161 tctacatcgc ctgcggcatc tccggagcca tccagcacaa ggcgggcatg cagtcgagtg
     2221 actacatcgt ggcgatcaac aaggaccccg atgctcccat cttcggcttc gccgatcttg
     2281 gagtcgtcgg tgacgtcttc gaggtggtgc cggcgttgac gcaggagatc cgcaagcgca
     2341 agtccgcggc atgacgcgag cgcccggcgc ccggcgcaag gaccaacgga ggtagggtcg
     2401 atgcagacgc gcgagttgct gttcaacgtt ccgctgtggg cggaggtcgc catgtacctg
     2461 ggggcggctg tcgccggcct catgctcgtg accgacctgg cgcgtcgcgt gggcatgtac
     2521 cgccggggtg ccgcggccgg agcgcgcgcg gatcatccgc tgcggcgggt cggcgacctt
     2581 ctcgtgaacg gcatcctgca gcgcgacctc ctgcgtgacc gttacgcagg cctcatgcac
     2641 ctgggtatct tcttcggatt cctggtgctg ctcatcggga ccaccatcct gatcttccag
     2701 atcgatctcg gcttggactt cttccatggg agcttctacc tggtcttcag cttcgtcatg
     2761 gagatcgcgg gcctggccct gctcgggggc gtgctgctgg ccgccttccg ccggtacgtg
     2821 cgcaggcccg cgcgcctcag ccgctcgtgg gatgatcact atgcgctcat cgtgctcttc
     2881 gtactcggcg tgaccggctt cctgctcgag ggggcccgca tctacgccga cggcttcccc
     2941 tcgttcgagc gaggcgccag cttcgtggga tacggggtgg gcaaggtctt ctcggtcttc
     3001 gccggcaggg acacgttcgt caccttgcac gagtggggct gggtcgtgca catggcgctg
     3061 gccgtcggtt tcgtgggact actgagctac acgaagttcc tgcactcgct caccagcccg
     3121 atcaacatcc ttcttcgcga acgacgcgca cggggttcca tgaccttcgt gcctgacgtc
     3181 gaggagaggg gg
//
LOCUS       DASRMJ010000176         9706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_13947, whole genome shotgun sequence.
ACCESSION   DASRMJ010000176 DASRMJ010000000
VERSION     DASRMJ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13947"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFD74_04835"
     CDS             complement(<1..437)
                     /locus_tag="VFD74_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HZK48908.1"
                     /translation="MEPADPADSADMDFDDSPHDECGVFGIYAPERDVARLTFFALFA
                     LQHRGQESAGIAVSDQTQIMVYKEMGLVSQVFKEQTLRSLSGQMAIGHVRYSTTGSSE
                     WRNAQPIHRARNGSAIALAHNGNLVNTDDLRQQLVAAGVELSST"
     gene            complement(496..2877)
                     /gene="purL"
                     /locus_tag="VFD74_04840"
     CDS             complement(496..2877)
                     /gene="purL"
                     /locus_tag="VFD74_04840"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZK48909.1"
                     /translation="MTELYADLGLTSAEYTRIKEIQGREPTHVELAVYSLMWSEHCGY
                     KHSRPYLGRFPSTGARVLQGPGENAGIVDIGGGLAVAMKIESHNHPSAIEPYQGAATG
                     VGGILRDIFAMGARPICSLDSLRFGEIAAAPRADADEGARGAAAGAGHDRQRYLFEHV
                     VAGIGGYGNCMGIPTVAGEVYFEDAYSGNCLVNAMTVGLVRPENIVRSAAAGVGNHVV
                     LFGSKTGRDGIGGASVLASQEFDETLEEKRPSVQVGDPFTEKKLLEVCLELLSDGRFV
                     ALQDLGAAGITSSASEMAAKGGVGIDIHTDRVPLRERDMAPWEIMISESQERMLAVVT
                     PEDWAYVKAACDRWDLDCTVVGDVTGSTRLRVFHGGEVQGDMPAEELADAPTCTVTVE
                     RPAHEVDEPVDPASYAPAPAPLGEILLDLLGAPNIASRRWIWEQYDHQVQLNTVVLPG
                     GDAALLRVKETGAGLAVCTDGNGRHTYLDPYRGAKAAVAEAARNVSCVGAEPIAVTNC
                     LNFGNPEKGAISYQLSRAIEGMAEACEAFGVPVISGNVSLYNESFGQAIYPTPVVGML
                     GLSDDFSVHCTSGFAAEGDVVVLLGDAAPALDGSEYQKRWYGVVEGRIPDVDLEAEVL
                     LQAVLRRAIGDGMVRSAHDCADGGLAVALAESCVAGGVGARLDLGGVATPAAAAPAPG
                     APVALDAAHATAGAASSAASSAVASAPTSPAARHDVALFGEAPTRVVVSVAPEAAAAL
                     LNLCAGAGVPVTTLGTVGGDTLRVRVGDDGLDIPVPALRDAHEGGIPRALGVG"
     gene            complement(3122..3844)
                     /gene="purQ"
                     /locus_tag="VFD74_04845"
     CDS             complement(3122..3844)
                     /gene="purQ"
                     /locus_tag="VFD74_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZK48910.1"
                     /translation="MTGIAPRKVRFGIVVFPGSNCEYDAKHALSTFPHAEPYYLWHKD
                     DDLQSPDVVILPGGFSYGDYLRTGAIARFSPLMGAVSAFAESGGLVLGICNGFQILTE
                     AHMLHGAMLRNRGLKFVCRSQHLRVERTDTPFTRGLTVGDVIQVPVNHNEGAWTAGSE
                     DLRQIEDQRLVVFRYCDEGGDVTDAVNPNGAVDNVAGIVNECGNVLGMMPHPERVCES
                     AVGGIGGRGLFDSLINHVLEGM"
     gene            complement(3841..4101)
                     /gene="purS"
                     /locus_tag="VFD74_04850"
     CDS             complement(3841..4101)
                     /gene="purS"
                     /locus_tag="VFD74_04850"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HZK48911.1"
                     /translation="MFRASVYVTPKAGILDPQGATIERALPALGFEGVQNIRVGRYVT
                     LDVQGADPEVVRRDVDDMCRRLLANPIIEDYRFELTALEERE"
     gene            complement(4125..4847)
                     /gene="purC"
                     /locus_tag="VFD74_04855"
     CDS             complement(4125..4847)
                     /gene="purC"
                     /locus_tag="VFD74_04855"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZK48912.1"
                     /translation="MTRGERLYEGKAKIVYATDDPNVYVQDFKDSATAFDGKKKGTIG
                     EKGRTNCAISSRLFELLEESGVPTHFLGREGDTGMRIRRLDMFQVEFVVRNVAAGSLS
                     KRIGYPEGTVLKNPPIVEYYYKDDALGDPLLCMAHLDELQVISPDDLAAATVLAKRVN
                     SILTTFFLERDLTLVDFKLEFGKDAAGAVRLGDEISPDTCRLWDATTNEKLDKDRFRR
                     DLGGVEEAYAEVLRRISGVGGV"
     gene            5066..5473
                     /locus_tag="VFD74_04860"
     CDS             5066..5473
                     /locus_tag="VFD74_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48913.1"
                     /translation="MRRPRGVIIIALLLLTQAAVWTATGTLYFSELPVFHLPAMETSA
                     FALALAGVNLIVAVGMVLLKPWAWVGAMTVQGLSLGAGLWSYSHGNHDYPLMVISVMA
                     VLYLNLREVRRPFGLEDLKLRWRDEPEEPHDLL"
     gene            5460..7292
                     /locus_tag="VFD74_04865"
     CDS             5460..7292
                     /locus_tag="VFD74_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48914.1"
                     /translation="MTFSEPRSAQPADDLSLLRRFEPQLRLNRGELFRPMDIETFLMH
                     TDLVVTRRNEPPLILETRPNVTQEILAGCGQVATPGKVHLRLVDRTLTALQLMSFHAR
                     SELREFRKGTGRHARVGLVARLLDVAFRVSLVARGRVPGGTAAAAAVAFRHMRDASPA
                     PTYYGRVLRTQFYTVLQYWFFYAYNDYRSHYHGANDHEGDWELVSIFLDEGGPEEATV
                     SWVAYANHDFEGADLRRRWDDPEVERIGEHPVVNVGAGSHSSYFSPGEYVIRIELPLL
                     AKASAAIATIRSVWQTVFRQGGVQSQRTGGDLNIAFVDYARGDGLRIGPEGDVGWDAR
                     LLDEQPALVHYRGLWGRYINDPIRGEDAPAGPRFNRDGTVRTVWSDPAGWAELTTVPP
                     ASREIPAAKAESKRLEAELAGLEREIAEAEAEAVRLGLRMDTLAAASPSGRQLDECVS
                     RRSEARDAVSRLREKASETEIMSWAMRLRLERLRAGDRGDPRAHLHHPAMPLGPGRLR
                     LHRIAETWSAVSAGVLLLLMSTSVIVYHAALLPLLLLSGAFLFVESLFHRRVAALVHG
                     IAMGLTLTTLGILLYEFWWQILAALVLGLGATITWANLRELTRR"
     gene            complement(7431..8732)
                     /gene="purB"
                     /locus_tag="VFD74_04870"
     CDS             complement(7431..8732)
                     /gene="purB"
                     /locus_tag="VFD74_04870"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZK48915.1"
                     /translation="MIERYSTPEMAALWTDDARMATWLEVEIAVCEAWAELGEIPAEA
                     VDEIKSRAAFDVARVQEIEETTQHDVIAFLTNVSEHVGEASKYIHFGMTSSDMLDTAL
                     ALQLKRAGALLAEQTRELGRVLQRRALEHRDTVMIGRSHGVHAEPITFGMVLGLWAFE
                     VARGLERLERATEQVAVGKISGAVGTYANIDPRVELSVTSRLGLGAAPISTQIVQRDR
                     HAEFMCALALLATTIEKIAVQVRHYQRTEVLEAEEYFAAGQKGSSAMPHKRNPIVSER
                     LAGQARVVRGNMLAAFENVPLWHERDISHSSVERVILPDSTILLHYMLKKATSLIDRL
                     LVYPEAMAANLQRSFGLIHSQHVLLELVRGGMMREDAYALVQRNAMRAWEEKRSFKEL
                     LLADAEVAEHLDSAAVEACFDPAHELRNVGVIFERVSALEW"
     gene            complement(8732..9235)
                     /gene="purE"
                     /locus_tag="VFD74_04875"
     CDS             complement(8732..9235)
                     /gene="purE"
                     /locus_tag="VFD74_04875"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZK48916.1"
                     /translation="MKSHAEPDVEAVLQAMEMDAPLVGVLMGSQSDAEVMQAASDELT
                     ARGISHEVNVLSAHRDPDGVREYALTAEARGIKVIIAGAGKAAALPGVVASFTELPVI
                     GVPIRTSDLGGMDSLLSIVQMPTGVPVACVAINGARNAAILAAKMLRLCWIAPGGGPS
                     DPPTVEF"
     gene            complement(9276..>9706)
                     /locus_tag="VFD74_04880"
     CDS             complement(9276..>9706)
                     /locus_tag="VFD74_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HZK48917.1"
                     /translation="FGDPETQALIPRLRSDLLEILLAAATGGDLPEAAEWTPGACVGV
                     VMASRGYPASSSHGDVIAGLDAAATLAGVEVFHSGTGTEGGDVVTAGGRVLTVSAVSD
                     SFVSARALAYAGVDRISFDGAQHRTDIARRAEDWERAAGA"
BASE COUNT         1514 a         3400 c         3192 g         1600 t
ORIGIN      
        1 gtggaggaca gttccacgcc ggcggcgacc agctgttggc gcaggtcgtc ggtgtttacc
       61 aggttcccgt tgtgggccag cgcgatggcc gagccgttgc gcgctcggtg gatcggctgg
      121 gcgttgcgcc actcgctgga gccggtggtg gagtagcgca cgtggccgat ggccatctgg
      181 ccggagaggc tccggagcgt ctgctctttg aacacctggg agaccagccc catctccttg
      241 tacaccatga tctgcgtctg gtcgctgacg gcgatgccgg ccgactcctg gccccggtgt
      301 tggagcgcga agagggcgaa gaaggtgagc cgggctacgt cgcgttcggg ggcgtagatg
      361 ccgaagaccc cgcactcatc gtgcggcgag tcgtcgaagt ccatgtcggc ggaatccgcc
      421 gggtcagcgg gctccatcag gtcagcgggc tccatcaggt cggccgactc catcgaatcg
      481 gcggaatcag ccaactcagc ccactcccag tgcgcgcggg atgccgccct cgtgcgcgtc
      541 gcgcagggcc ggcaccggga tgtcgagccc atcgtcgccc acccgcacac gcagcgtgtc
      601 tcctcccacc gtcccgaggg tcgtgacggg cacgcccgcg cccgcgcaga gattcaggag
      661 agccgcggct gcctcgggtg caacgctcac gaccacccgc gtgggcgcct cgccgaacag
      721 tgccacgtcg tggcgcgcgg ctggagacgt cggcgcggac gcgaccgcgg acgaggccgc
      781 agacgaggcc gcgccggcgg tggcatgcgc ggcatcgagg gcgactggcg cccccggtgc
      841 aggcgccgcg gccgcaggtg tcgccacgcc gccgaggtcg agtcgtgcgc ccactccgcc
      901 ggccacacag ctctcggcca gcgcgacggc gagtccgccg tcggcgcagt cgtgggccga
      961 tcgcaccatg ccatcgccga tcgcccgccg cagaacggcc tgcaacagga cttcggcctc
     1021 gaggtccacg tcggggatgc ggccttccac cacgccatac cagcgcttct ggtactcgga
     1081 gccgtcgagc gcgggcgcgg cgtcgcccag taggaccacc acgtcaccct cggccgcgaa
     1141 cccgctcgtg cagtggacgc tgaagtcgtc gctcagacca agcatcccca cgaccggcgt
     1201 cgggtagatc gcctggccga aactctcgtt gtagagagag acgttgcccg atatcaccgg
     1261 gacaccgaac gcctcgcagg cctccgccat gccctcgatc gcccggctca gctggtagga
     1321 gatggcgccc ttctcaggat tcccgaagtt gaggcagttg gtcacggcga taggctccgc
     1381 acccacgcag gagacgttcc gcgccgcctc ggccaccgcc gccttcgctc cccggtatgg
     1441 gtcgaggtag gtgtgccgtc cgttgccgtc ggtgcacacg gccaaccccg ctccggtctc
     1501 cttcacccgc agcagggcgg cgtcaccgcc gggcagcact accgtgttca gctgcacctg
     1561 atgatcgtac tgttcccata tccatcggcg ggaggcgatg ttgggcgccc ccagcagatc
     1621 gaggagtatc tcgccgagcg gcgcgggcgc gggcgcgtag gaggccggat cgacgggctc
     1681 atcaacctcg tgcgccggcc tctccaccgt caccgtgcag gtgggggcgt cggccaactc
     1741 ctccgccggc atatcgccct gcacctcgcc gccgtggaac acccgaagcc gagtggagcc
     1801 ggtgacgtcc ccgacgacgg tgcagtcgag gtcccagcgg tcacacgccg ccttgacgta
     1861 ggcccaatcc tccggggtca cgaccgccag catccgctcc tgactctcgc tgatcatgat
     1921 ctcccacggc gccatgtcgc gctcgcgcag aggcacccgg tcggtgtgga tgtcgatgcc
     1981 gacgccgccc ttggccgcca tctcgctcgc cgaggaggtg atgccggccg cgccgaggtc
     2041 ttgcagggcc acgaagcgcc cgtcgctcag cagctccaga cacacttcca acagcttctt
     2101 ctcggtgaac gggtcgccca cctggaccga gggccgcttt tcctcgaggg tctcgtcgaa
     2161 ctcctggctg gcaaggacgc tcgcaccccc gatgccgtca cgcccggtct tggagccgaa
     2221 gagcacgacg tggttgccca ctcccgcggc tgcgctgcgc acgatgttct cggggcgcac
     2281 cagccccacc gtcatggcgt tcaccaggca gttgcccgag taggcgtctt cgaaatacac
     2341 ttcacccgcc acggtgggga tgcccatgca gttgccgtag cctccgatcc ctgccacgac
     2401 gtgctcgaag agatagcgct gccggtcgtg gccggcgccg gcggccgctc cccgcgcccc
     2461 ctcatcggcg tccgcccgcg gcgctgcggc tatctccccg aaacgcaagg agtcgaggct
     2521 gcagatgggc cgggcgccca tggcgaagat gtctctgagg atgcccccca cgcccgtagc
     2581 ggcgccctgg tagggctcga tcgccgaggg atggttgtgg ctttcgatct tcatggcgac
     2641 ggccaggccg ccgccgatgt caacgatacc ggcgttctca cccggcccct ggaggacgcg
     2701 agccccggtg gaagggaagc ggcccaggta gggccgcgag tgcttgtatc cgcagtgttc
     2761 gctccacatg agcgagtaga cggcgagctc cacgtgcgtg ggctcgcgac cctggatctc
     2821 tttgatgcgc gtgtattcag ccgaggtgag accgaggtca gcgtacagct ccgtcatgcg
     2881 ttctcccgcc cgccctcgcc gggagtgggc gaagcccccg cgggcgcgga ggcggtggaa
     2941 gcatcgcccg gccgcgcttc cggcgcagcc ggggaggcgg cgggccggca gcccggcccc
     3001 attcctggta tctcgcgcca gtcgagctgg ctctggcaca tccggcagac gcaggtgtcg
     3061 ggcaagaagc cttcgatgaa ggtgtcggcg tggccgcacc gggggcagac gacaggtgac
     3121 atcacattcc ctccaacacg tggttgatga gagagtcgaa aagaccgcga ccgccgatcc
     3181 cgcccactgc gctctcgcac acgcgctcgg gatggggcat catcccgagt acattgccgc
     3241 actcgttgac gatgccggcc acgttgtcca cagccccgtt ggggttgacc gcgtcggtga
     3301 cgtcgccgcc ttcgtcgcag tatcggaaca ccacgagccg ttggtcctcg atctgccgaa
     3361 gatcctcgct gcccgccgtc cacgcgccct cgttgtggtt caccggcacc tggatcacgt
     3421 cgcccacggt caggccgcgc gtgaacgggg tgtcggtgcg ctcaacacgc aggtgttggc
     3481 tgcggcagac gaacttcaag ccgcgattcc ggagcatggc accgtgcaac atgtgcgcct
     3541 cggtcaggat ctggaaaccg ttgcatattc ccaacaccag cccaccgctc tccgcaaagg
     3601 cggagaccgc gcccatgaga gggctgaagc gggcgatcgc gccggtgcgc aggtagtcgc
     3661 cgtaggagaa tccgcccggc aggatcacaa cgtcgggcga ctgaaggtcg tcgtccttgt
     3721 gccacaggta gtagggttct gcgtggggga acgtgctcag ggcgtgcttg gcgtcgtact
     3781 cgcagttcga cccagggaac acgacgatgc cgaaccggac cttcctcgga gcgatcccgg
     3841 tcactcccgc tcctcgagcg cggtcaactc gaagcggtag tcctcgatga tggggttggc
     3901 cagaaggcgc ctgcacatgt cgtcaacgtc tcggcgaacg acttccggat cggcgccctg
     3961 gacatcgaga gtcacgtatc gacccacccg tatgttctgg actccctcga aacccagcgc
     4021 gggcagcgcc ctctcgatgg tcgcaccctg gggatccagg atcccggcct tgggcgtcac
     4081 gtagactgat gctctgaaca tgcctcgtct cctccgattc agcgtcagac tcccccgact
     4141 ccggagatgc ggcgaagcac ctcggcgtac gcttcttcga ccccgcccag gtcgcggcgg
     4201 aaacgatcct tgtcgagctt ctcgttcgtg gtcgcgtccc agaggcggca ggtgtccggc
     4261 gagatctcgt cacccaagcg gacggccccc gcggcgtcct ttccgaactc cagcttgaaa
     4321 tcgaccaagg taagatctct ctccaagaag aaggttgtaa gaatgctgtt cacccgcttc
     4381 gccaacaccg tcgcggcagc caggtcgtcg ggtgagatca cctggagttc atcgaggtgg
     4441 gccatgcaca gcagagggtc accgagtgca tcgtccttgt agtagtactc gacgatcggc
     4501 gggttcttga gcaccgtgcc ctccgggtag ccgatgcgct tggagagcga cccggccgcc
     4561 acgttgcgca ccacgaactc cacctggaac atgtcgagtc tgcgtatgcg catgcccgtg
     4621 tccccttcgc ggcccaggaa gtgggtgggg accccggact cctcgagaag ctcgaacagc
     4681 cgggacgaga tggcgcagtt cgtgcggccc ttctcgccga tggtgccttt cttcttgccg
     4741 tcgaaagcag tggccgaatc cttgaaatcc tggacgtata cgttcggatc atccgtggcg
     4801 tacacgatct tggccttgcc ctcgtagagt ctctcgcctc gagtcatctg cgctcctgcg
     4861 ggtcgtgcgt ggtccccatg cggctgagcc gacagggtag attctaccag gtcacgatgc
     4921 ggcggccggg ccgcccgagg ggccgggcac gaagcaaacc ccgccgggtc gcccgagggg
     4981 ccggacgcga cggaaaccat ggcgcaccgc ccgcgatagc ccaaacgttg tatcctcacc
     5041 tcatatggct tggacacgcc cccgagtgag acgcccgcgc ggcgtcatca taatcgccct
     5101 gctccttctg acgcaggccg ctgtgtggac cgccacggga acgctgtatt tctccgagtt
     5161 gccggtcttc catctgccgg ccatggagac ctcggccttc gcactggcgc tcgcgggggt
     5221 gaacctcatc gtggccgtgg gtatggtcct gctcaaaccc tgggcttggg tcggagccat
     5281 gaccgttcag ggactctcgc tcggcgcggg cttgtggtca tactcgcacg gcaaccacga
     5341 ctacccactc atggtgatct cggtcatggc cgtgctctac ctgaaccttc gcgaggtgcg
     5401 acgtccgttc gggctcgagg acctgaagct gcgttggcgc gacgagccgg aggagccgca
     5461 tgaccttctc tgagccgcgg tctgcccagc cggcggatga cctctcgctt ctgcgccggt
     5521 tcgagccgca gttgcgcttg aaccggggcg agctcttccg ccccatggat atcgagacgt
     5581 tcctgatgca caccgatctg gtggtgaccc gccggaacga gccgccgctg atcttggaga
     5641 cgcgcccgaa cgtaacccag gagatcctcg ccgggtgcgg tcaagtcgcc actccgggca
     5701 aggtccacct gcgcttggtc gaccgcactc tgaccgccct ccagctgatg agcttccacg
     5761 ctcgctcgga gttgcgcgag ttcaggaagg gcaccggccg tcacgcccgc gtgggactgg
     5821 tcgcccgtct gcttgacgtg gccttccgcg tctcgctcgt ggcccgaggc cgagtccccg
     5881 gcggcacggc cgcggccgcg gccgtcgcct tccggcacat gagggatgcg tcgcccgccc
     5941 ccacgtacta cgggcgggtg ctcaggaccc agttctacac ggtgctgcag tactggttct
     6001 tctatgccta caacgactac cgcagccact accacggagc caacgaccac gaaggcgact
     6061 gggaactcgt gtcgatcttc ctcgacgagg gcggccccga agaagcgacg gtgtcgtggg
     6121 tcgcgtacgc caaccacgat ttcgaggggg ccgacctacg ccgccgctgg gacgacccgg
     6181 aggtggagcg catcggcgag catccggtgg tcaatgtggg ggcaggttcc cactcctcct
     6241 acttctcccc cggcgagtat gtcatcagga tcgagctccc cctgctggcc aaggccagcg
     6301 ccgccatcgc caccatccgc tcagtgtggc agacggtctt ccgccaaggt ggagtgcaaa
     6361 gccagcgcac cggcggtgac ctcaacatcg ccttcgtcga ctacgcacgc ggggacggac
     6421 tcaggatcgg acccgagggc gacgtgggct gggacgcgag gctccttgac gagcagcccg
     6481 ctctcgtcca ctaccgcggc ctctgggggc gctacatcaa cgacccgatc cgtggcgagg
     6541 atgcacccgc agggccgcgc ttcaaccggg acggcacggt gcgcacggtc tggtctgatc
     6601 ccgcgggctg ggcggaactc accaccgtcc cccccgcttc gagggagata cccgcggcca
     6661 aagcggagtc gaagagactc gaggcggaac tcgccggtct ggagcgcgag atcgccgagg
     6721 ccgaagcgga ggccgttcgg ctggggttgc gcatggatac gctggccgcc gcgtccccat
     6781 cgggacggca gttggacgag tgcgtatcgc ggcgatccga agcccgcgat gcggtgagcc
     6841 gtctacgcga gaaggcgagc gagaccgaga tcatgagctg ggcaatgcga cttcgtctcg
     6901 aacggctgcg ggcgggcgac cgtggagacc cacgggccca cctccatcac ccagcgatgc
     6961 ccctgggacc cggccgcctg cgcctgcacc ggatagctga gacctggagt gccgtcagcg
     7021 ccggggtgct cctcctgctg atgtccacga gcgtgatcgt ctaccatgcg gcgctgctgc
     7081 cgctgttgct gctgtcgggc gcctttctct tcgtggagtc cctgttccac cggcgagtgg
     7141 ccgccctcgt gcacgggata gcgatgggtc tgacgctgac gacgctcggc attctgctct
     7201 acgagttctg gtggcagatc ctggcggccc tggtcttggg cttgggagcc accatcacct
     7261 gggcgaacct gcgcgaactc acgcggcgct gaccgggccg gcgcccgtcg ccgcgcccga
     7321 tgcgcggtag taccgcccgc gcccgtcggc ggccccgcgg tagtcccgcc cccgcgcgta
     7381 gggcggcacg agatgcggcg cgccgcgcgc cgcacgccgc acgccgccgg tcaccactcg
     7441 agcgcagaga ccctctcgaa gatcacgcca acgttgcgga gttcatgtgc ggggtcgaag
     7501 cacgcctcga ccgcagctga gtcaagatgc tccgcgacct ccgcgtcggc caggagcagc
     7561 tccttgaacg agcgcttctc ctcccaggct ctcatggcgt tgcgctgcac gagggcgtag
     7621 gcgtcctccc gcatcatccc gccccgcacg agttcgagca gcacgtgctg gctgtggatc
     7681 agtccgaagc tgcgctgcag gttcgcggcc atcgcttccg gatacacgag cagacggtcg
     7741 atcaggctgg tggccttctt gagcatgtag tgaagaagga tcgtgctgtc ggggagtatg
     7801 accctttcga cgctcgagtg gctgatgtcc cgctcgtgcc acagcggcac gttctcgaac
     7861 gcggccagca tgttccctct gacgacgcgg gcctgcccgg ccagacgctc cgagacgatc
     7921 gggttgcgct tgtgcggcat ggccgacgaa cccttctgtc ccgccgcgaa gtactcctcg
     7981 gcctccagca cctcggtccg ctggtagtga cgcacctgca cggcgatctt ctcgatcgtg
     8041 gtcgccagca gggcaagcgc gcacatgaac tcggcgtggc ggtcacgctg cacgatctgc
     8101 gtggatatgg gtgcggcgcc gaggcccagg cgggaggtca ccgatagttc cacccgcggg
     8161 tcgatgttgg cgtaggtgcc cacggcgccc gagatcttgc ccacggccac ctgttcagtg
     8221 gcccgctcca gccgttcgag accgcgcgcc acctcgaagg cccagagtcc caagaccatc
     8281 ccaaaggtga tgggctccgc gtgcaccccg tggctgcgac cgatcatgac agtgtcgcgg
     8341 tgctccaacg cgcggcgttg gagcacccgg ccgagctcgc gcgtctgctc ggcgagcagg
     8401 gcgccggcgc gcttgagctg cagggccagt gccgtgtcca gcatgtcgga agaggtcatg
     8461 ccgaagtgga tgtacttgct cgcttcgccc acgtgttcgc tgacgttggt gaggaaggcg
     8521 atgacgtcgt gttgggtggt ctcctcgatc tcctgcacgc gggccacgtc gaaggcggcg
     8581 cgagacttga tctcgtccac ggcttcggcg gggatctccc ccagctcggc ccacgcctca
     8641 cacacggcga tctcgacctc caaccaggtg gccatgcggg cgtcgtcggt ccacagggcc
     8701 gccatctcgg gagttgagta gcgctctatc atcagaactc caccgtgggg gggtcggagg
     8761 gtccgccacc cggcgctatc cagcagagcc ggagcatctt ggcggcgagg atggccgcgt
     8821 tgcgggcgcc gttgatcgcc acgcaggcca ccgggacgcc tgtcggcatc tgcacgatcg
     8881 acagcagcga gtccatgccg cccaggtcag aagtccgaat aggaacgccg atcaccggaa
     8941 gctcggtgaa cgacgccacc acacccggaa gcgccgccgc cttgccggca cccgcgatga
     9001 tcaccttgat gccgcgggct tccgcggtga gcgcgtactc gcgcacgccg tcggggtccc
     9061 ggtgggcgga gagcacgttc acctcgtgcg agatgccgcg ggcggtcagc tcgtccgacg
     9121 ccgcctgcat cacctcggcg tccgactgcg agcccatcag gacgcccacc agcggcgcgt
     9181 ccatctccat ggcttggagg acggcctcta cgtcaggttc agcgtgagat ttcatcgatc
     9241 tccccctcgt atgacggtgc tcgggcgccg gcgcctcatg cgcccgcggc cctctcccaa
     9301 tcctcagccc gccgtgcgat atccgtccgg tgctgcgccc cgtcgaacga tatacgatcg
     9361 acgccggcgt acgccaacgc gcgcgcggat acgaacgagt cgctcacggc gctcacggtg
     9421 aggacgcgcc ctccggccgt caccacgtcg ccgccctcgg tcccggtgcc cgagtggaac
     9481 acctccactc cggcgagagt cgcggcggcg tccagcccgg cgatgacgtc cccatgcgag
     9541 ctcgaggccg ggtacccacg cgaagccatc accactccca cacacgcacc cggagtccat
     9601 tcggccgcct ccggcaggtc cccacccgtg gccgcggcca agagtatctc gagcaggtcg
     9661 gagcgcagcc gtgggatcag cgcctgcgtc tctgggtcgc cgaacc
//
LOCUS       DASRMJ010000177         6520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_233829, whole genome shotgun sequence.
ACCESSION   DASRMJ010000177 DASRMJ010000000
VERSION     DASRMJ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233829"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2139
                     /locus_tag="VFD74_04885"
     CDS             <1..2139
                     /locus_tag="VFD74_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48918.1"
                     /translation="LVSVGLVFGLVYGLLLAFYRPDLMLSNTITSGGDMGAHHYAAKY
                     MIDVLVPSFHLTGWTMGWYAGMPLFTFYLPLPFLMIAIFNFVLPYGVSFKLATVLGVF
                     LLPLAAYLLGRLFRFREPFPLLAAVMSLAFLFSESFSIYGGNILSTLAGEFGYSLAFA
                     LIFIFLGTLYRGMERGRFDWLFAVNALILMGVVLSHIVATIVLIIIVPSFLLVHRTKK
                     AFVYLAGVFFVGFLLTAFWGLPFVDKLSWTAHMSWGQLTLFKDLLPPELRPVAALGVV
                     GMAFALAKRDVKTLPLLWIVVFVVALFFTLPDGRLWNGRLVPFMYFTICLWAAYGLAW
                     LLRAFSVMLEDLLGAGPRVSRLVYSPVIAATMVAIIFVMSGTAAGWIKWNYSGYEAKE
                     PWPRLEEILKTIDELPPGRVMWEHSPSLDKFGTPRVFELIPFWTSHPTMEGTLMESAF
                     TAPYHFVNQGELSMKPSHAIIGVDYPPRNTAAGLTHLAFMNIPYLLTVSPEVTLEVEA
                     DPRADLIAKIDDVSIFRVSGSEGYVQVARNLPLKVKTDDWRGTIVPWYKNLNALPMPI
                     IWDRGEKGLDQFESIDRADVANPPATPIEGDGAVLSEQIENDRISFETTAIGKPHLIK
                     VSYFPNWKVKGADGPYVVSPSFMMVIPRQREVTLYYGRTASNVVGQAFTIVGWLIVVA
                     VLAAEVLRRRRRNGGHGPSDRRLGAPPSEE"
     gene            complement(2231..2512)
                     /locus_tag="VFD74_04890"
     CDS             complement(2231..2512)
                     /locus_tag="VFD74_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48919.1"
                     /translation="MRFTDLFSGKSWPASEAEVRLLVEFYSEAENLNDDFGTTPPARI
                     DIVVKSGENLVVWGGGQTYQTVCKGDREYNIESSGLHQMLQEIVARGQR"
     gene            complement(2894..3859)
                     /locus_tag="VFD74_04895"
     CDS             complement(2894..3859)
                     /locus_tag="VFD74_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK48920.1"
                     /translation="MEFRLRPLAKKVLPQAVVDALRSQALNLPHAAERIRAQRAFSAA
                     TNAPPYLGVDVLETLQNKYSPPPEYGWDERAVEVRGMQRAARILRLPGARLADSFLEL
                     GCWDGMVCSGLCLEGKSATAIDSRDDGFDARASRAGARLLRMDAAQLDFADECFDFVY
                     SYDAFEHFASPEAVLREAIRVVKAGGFIHLDFGPLYYSAMGEHAYNSITVPYCQHLFP
                     RQMLNDFATHRGLKPIRPGRVNGWALESYRQLWDKYDYILKKVRYRERLDLSHVDLIR
                     MFPSCFKSKSQHFDGFVVASISALFRKSSNELGEGETPSSGQLPG"
     gene            complement(4010..4082)
                     /locus_tag="VFD74_04900"
     tRNA            complement(4010..4082)
                     /locus_tag="VFD74_04900"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4047..4049),aa:Ala,seq:cgc)
     gene            complement(4216..4449)
                     /locus_tag="VFD74_04905"
     CDS             complement(4216..4449)
                     /locus_tag="VFD74_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HZK48921.1"
                     /translation="MCLAIPARIVSIDGIMGIIDLEGVRREVSLILCPEAVVGEYALV
                     HAGFAISVIDEEQARLGLEAFDEFRRLLEEGAA"
     gene            complement(4468..5292)
                     /locus_tag="VFD74_04910"
     CDS             complement(4468..5292)
                     /locus_tag="VFD74_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZK48922.1"
                     /translation="MQFFTSAIAIIVVLVALISSAVKVVPEYQRGVIFRLGRLIGAKG
                     PGLFLLIPIVDRMVKIDLRTITMDVPPQEAITKDNVPVKVNAVVYFRAIDPNRAVVEV
                     ENFIVATSQIAQTTLRSVLGQSDLDHLLTEREKINQRLQEIIDEQTDPWGIKVSIVEI
                     KDVEVPASMQRAMARQAEAERERRAKVIAAEGEFQASEKLAQAAEILDRTPTALQLRY
                     LQTLVEIGSEKNTTTVFPIPLDLFKPFLDRALTPPRPPAAASVIAPSGAAAPVVES"
     gene            complement(5317..>6520)
                     /locus_tag="VFD74_04915"
     CDS             complement(5317..>6520)
                     /locus_tag="VFD74_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HZK48923.1"
                     /translation="SAYLVRTMERARAAGAEVLVIEIDTPGGLDSSMREIIQAEIDSA
                     MPVVIYVYPQGARSASAGVYIMMGADVAAMAPQTNLGSAHPVSLTGEMDEEMKKKVTN
                     DAAAYIRGLAATHGRNTGWVEQAVRESVSLTAEEAKAQNVIEFVVADLDDLLAQLDGY
                     TTTPKGVTLHTAGAPVVEVSMSWREKLLHRLVDPNIIFIFMLLGVYGLIFEFQNPGLG
                     APGIAGAISLLLAAYGLQILPVNYVGLLLLVVAMVLYVAEVKVQSSGVLAFGGTVALI
                     LGGLLLFNAPGSVIRVDWTVIAIMALASLAFFGFVVSAVAAAHRRKPATGSEAMMGEQ
                     GTARSRLDPSGQVAVHGEIWQADAEGDPIEPGTPVTVTGIRGLRLQVTAAKGAGDKAR
                     ETGSTDHD"
BASE COUNT         1108 a         2084 c         2031 g         1297 t
ORIGIN      
        1 ctggtgtcgg tgggcctggt gttcggactc gtctacgggc tgctcctcgc cttctaccgc
       61 cccgacctca tgctctccaa cacgatcacc tccggcggcg atatgggagc ccaccactac
      121 gccgccaagt acatgatcga cgtgttggtg ccgagcttcc acctcaccgg ctggacgatg
      181 gggtggtatg cggggatgcc gctcttcacc ttctacctgc cgttgccgtt cctgatgatc
      241 gccatcttca acttcgtcct gccgtacggg gtctcgttca agctcgccac cgtcctgggc
      301 gtgttcctgc tgccgttggc cgcgtacctg ctgggtaggc tcttccggtt tcgagagccc
      361 ttccctctgc tggcggcggt catgtcgctg gcgtttctct tctcggagag cttctccatc
      421 tacgggggca acatccttag cacgctcgcc ggggagttcg ggtattcgct tgccttcgcc
      481 ctgatcttca tcttcctcgg caccctgtat cgggggatgg agcgggggcg gttcgattgg
      541 ctgttcgcgg tgaacgcgct catcctgatg ggcgtcgtcc tttcgcacat cgtggcgacc
      601 atcgtcctga tcatcatcgt ccccagcttc ctgctggtcc accggaccaa gaaggcgttc
      661 gtctacctgg cgggcgtctt cttcgtgggc ttcttgctca ccgcgttctg ggggcttccg
      721 ttcgtggaca agctgtcgtg gaccgcccac atgtcgtggg gccagttgac cctcttcaag
      781 gatctgctcc cgccggaatt gcgtccggtc gcggcgctcg gcgtggtcgg gatggccttt
      841 gcgctcgcca agcgcgacgt taagaccctg ccgctgcttt ggatcgtggt gttcgtcgtg
      901 gcgctgttct tcacgttgcc cgacggccgt ctgtggaacg gacgcctcgt ccccttcatg
      961 tacttcacca tctgcctgtg ggccgcctac gggctcgcgt ggctgctcag ggccttctcg
     1021 gtcatgctgg aagatcttct gggggcaggg cctcgggtgt cgcgattggt ctactcaccg
     1081 gtgatcgctg cgacgatggt ggccatcatc ttcgtcatga gcggcaccgc cgccgggtgg
     1141 atcaagtgga actactcggg ttacgaggcc aaagagccgt ggccgcgcct cgaggagata
     1201 ctcaagacca tcgacgagct gcccccgggg cgggtgatgt gggagcactc tccttcgctc
     1261 gacaagttcg gcacgccgcg cgtgttcgag ctcatcccct tctggacctc gcatcccacc
     1321 atggagggca cgctgatgga gtcggccttc acggccccct accacttcgt caaccagggc
     1381 gaactctcga tgaagccgag ccacgccatc atcggggtcg actacccgcc gcgcaacacg
     1441 gccgccggac tgacgcacct tgcattcatg aacatcccct acctgctcac ggtctcgccg
     1501 gaggtcacgc tggaggtgga ggccgatccg cgtgccgacc tcatcgccaa gatcgacgat
     1561 gtgagcatct tcagggtgtc cggcagcgaa ggctacgtgc aggttgcgcg caacctgccg
     1621 ctcaaggtga agaccgacga ttggcggggc accatcgtcc cctggtacaa gaacctgaac
     1681 gccctaccta tgccgatcat ctgggaccgt ggtgagaagg gcctcgatca gttcgagtcg
     1741 atagaccgcg ccgacgtcgc caacccgccc gcgaccccga tcgagggcga cggcgcggtg
     1801 ctaagcgaac agatcgagaa cgaccggatc agcttcgaga ccaccgccat cgggaagccg
     1861 cacctgatca aggtgtccta cttccccaac tggaaggtca agggcgccga cggcccctac
     1921 gtggtctccc cgtcgttcat gatggtgata ccgcggcagc gggaggtcac gctgtactac
     1981 ggccgcaccg cgagcaacgt ggtggggcag gcgttcacga tagtggggtg gctgatcgtc
     2041 gtggcggtcc tggcggccga ggtgctgcga cggcgccggc ggaacggagg acacgggcct
     2101 tccgatcgcc gcttgggcgc tcccccgagc gaggagtgat cgtgaaagtg ggccgtactg
     2161 gacagtctgc aaacactgca caaccctatt gacggaagag ctgggttacg tgggcggcga
     2221 ctctggtagt ttaacgctgc ccacgagcca cgatctcttg cagcatttgg tgtaatccgg
     2281 agctctcgat gttgtattcc ctgtcgcctt tgcagacggt ctggtacgtc tgccctccac
     2341 cccacaccac cagattctcg ccggacttga ccactatgtc gatgcgggcg ggtggggtgg
     2401 ttccaaagtc atcgttcagg ttctcagcct ccgagtagaa ctccacgagc agtcgaactt
     2461 cggcctcgga ggcgggccaa gacttgcctg agaagaggtc tgtgaatctc acttcgcgga
     2521 tttcgtcgag ggatagactc ggctgacgtc cgcaagcagt gagagcgaac gcgaccaaaa
     2581 acagcatcag gcagagtgcc gttaccgaca cgacctcatg cttgcagttg ccccgacttg
     2641 cgctcatggg aacagcataa cccggccgcg aactcgctcg ggttctaaga cccccaaaga
     2701 actgtgcggc cgctcgtgat tgcactcccg gcgccgctgc tctcccagag ctttggcttc
     2761 tcgtaggttg gcgaagccgt tctcccaagg gggcgccggg ctccactgtg acgaactcct
     2821 ccgctgccca ttcttctcgc gggtgtactg tgaggcaaac gtatagttga ccagataggc
     2881 cttcttgtgt tcgtcaaccc gggagttgac cacttgacgg cgtttcgccc tcacccaatt
     2941 cgttgctcga cttccgaaac agtgcggaga tgctcgccac aacgaagccg tcaaaatgtt
     3001 gcgacttgct cttgaaacaa gagggaaaca tgcgaatcag gtcaacgtgg gacaagtcca
     3061 gtctttcgcg ataccgaacc ttctttagta tgtagtcata cttgtcccat agctgtctgt
     3121 agctctcaag ggcccaacca ttgacgcgac ccgggcggat gggcttcaag ccacggtgcg
     3181 tcgcgaaatc gttgagcatc tgtctaggga acaggtgttg acaatacggc acggtgatcg
     3241 agttgtatgc atgttcgccc atcgccgagt agtacagagg gccgaaatcc aggtgtatga
     3301 atcccccggc cttcaccact cggatcgctt cgcggagtac agcttccggt gaagcgaaat
     3361 gctcaaaggc gtcgtaggag tatacgaagt caaagcattc gtcggcgaag tccagctgcg
     3421 ccgcgtccat ccgcaggagt cgcgcgcccg cacgcgaggc gcgagcgtcg aatccgtcat
     3481 ctctggagtc gattgccgtc gcgctctttc cctcaaggca gaggccgctg cagaccatac
     3541 catcccaaca cccgagttca agaaaggagt ccgccagcct tgctcctggt agacgaagta
     3601 ttcgcgctgc tctttgcata ccccggactt ccacggcccg ttcatcccag ccgtattcgg
     3661 gtggagggga gtacttgttc tggagcgttt ccagcacgtc aacgccgaga tagggcggtg
     3721 cattcgtcgc tgccgaaaag gcgcgctgtg ctctgatcct ctccgccgcg tgcggcagat
     3781 tcaaagcttg agatcgcagg gcgtcgacga cggcctgcgg aagcaccttc tttgccagtg
     3841 gcctgagccg gaactccatg cagagcggcc tcccgcgggg catcgcgcgt gcaatctatg
     3901 ttcggggcgt tgggcgtcgc gaaggcagcg tgcgactctt tcgggtgcgc ggtcgccggg
     3961 caactcggca cccgctcggt gtccgtggaa acgcccggga ctaccaaggt ggaggtaagg
     4021 ggattcgaac ccctggcctt cgcattgcga acgcgacgct ctcccagctg agctataccc
     4081 ccacgtgaag tatagggcgg tatatatagc ctatcggccg ttcgcctgca aggcccaccg
     4141 gcgacccaga tcccgggcga agatcccggg cgaacacgaa cgacgtcgcg ttggcggcgg
     4201 cggtgcgctc gggactcagg cggccccttc ttcgagcagg cgccggaact cgtcgaacgc
     4261 ctcgagtccc agtcgcgcct gctcttcgtc gatgacgctg atggcgaacc cggcgtgcac
     4321 caaggcgtac tcgcccacga cggcttcggg gcagagtatg agcgacacct cgcggcgcac
     4381 gccctccaag tctatgatgc ccatgatgcc gtcgatactg acgatgcggg ccgggatcgc
     4441 aaggcacatg tgttcggacc tgcctggcta gctctcaacc accggggcgg ctgcgccgga
     4501 cggggcgatg acgctcgccg cggccggtgg acggggcggc gtgagggcgc gatccaggaa
     4561 cggcttgaac aggtcgagcg ggatggggaa gacggtggtg gtgttcttct cggagccgat
     4621 ctctacgagg gtctgcaggt aacgaagttg cagcgccgtg ggagtcctgt cgagtatctc
     4681 ggcggcttgg gcgagcttct ctgaagcttg gaactcaccc tcggcggcga tgaccttggc
     4741 ccgccgctcc cgttcggcct ccgcctgccg ggccatggcc cgttgcatgc tggcgggcac
     4801 ctcgacgtcc ttgatctcga cgatggagac tttgatgccc cacgggtcgg tctgctcgtc
     4861 gatgatctcc tggaggcgct ggttgatctt ctcgcgctcg gtgagcagat ggtcgaggtc
     4921 ggactggccg aggacgctcc tcagcgtggt ctgggcgatc tgggaagtgg cgacgatgaa
     4981 gttctccacc tcgacgaccg cgcggttggg atcgatcgcg cggaagtaga ccacggcgtt
     5041 caccttcacc ggcacgttgt ccttggtgat ggcctcctgg gggggcacgt ccatggtgat
     5101 ggtccgcagg tcgatcttga ccatgcggtc gacgatgggg atgagcagga agaggccggg
     5161 acctttggcc ccgatcagcc ggccgagcct gaagatgacg ccccgctggt attccggtac
     5221 cactttgaca gccgagctga tcaacgccac cagtactacg atgatcgcta ttgccgatgt
     5281 gaagaactgc atgatgctcc tctcgcgtcc gttgtgtcag tcgtgatcgg tcgaacccgt
     5341 ctcgcgggcc ttgtctccgg cgcctttcgc cgccgtcacc tgcagtctca aaccgcggat
     5401 gcccgtcacc gtgaccggcg ttccgggctc tatcgggtct ccctccgcgt cggcctgcca
     5461 gatctcacca tgcacggcca cctggcctga cgggtcgagg cggctgcgcg cggtgccctg
     5521 ctcccccatc atggcttcgc tacctgtggc gggcttccgt ctgtgtgccg cggcgaccgc
     5581 gctcaccacg aagccgaaga aggccagcga cgcgagcgcc atgatggcta tgaccgtcca
     5641 atccacgcga atcaccgatc ccggagcgtt gaacaacaac aatccaccga ggatcagagc
     5701 cacggtgcct ccgaaggcga ggacaccgct gctctgcacc ttgacttccg ctacgtagag
     5761 taccattgcc accaccaaca gaagcaggcc cacgtagttc accggcagta tctggagccc
     5821 gtaggcggcc agcaagagcg agatcgcgcc tgcgatgccc ggggcgccga gacccggatt
     5881 ctggaactcg aagatgagtc cgtacactcc cagcagcatg aagatgaaga tgatgttggg
     5941 gtcgaccagt cggtgcagta acttctcccg ccagctcatc gagacctcga ccaccggtgc
     6001 tcccgcggtg tgcagcgtca cgcctttggg tgtggtggtg tagccgtcca gctgtgccaa
     6061 gaggtcgtcg aggtcggcga cgacgaactc gatcacgttc tgcgccttgg cctcctcggc
     6121 cgtcagagaa accgactccc gcaccgcctg ctccacccac ccggtgttgc ggccgtgcgt
     6181 ggccgccaac ccgcggatgt aggcggccgc gtcgttggtg accttcttct tcatctcctc
     6241 gtccatctcc ccggtgaggg acaccgggtg ggccgagccc agattggtct ggggcgccat
     6301 ggccgcgaca tcggcgccca tcatgatgta gacgccggcc gaggcggacc tcgccccctg
     6361 cgggtagacg tagatgacca ccggcatggc ggaatcgatc tcggcttgga tgatctcgcg
     6421 catcgaactg tctaggccgc cgggggtgtc gatctcgatc acgagaacct cggctcccgc
     6481 cgcgcgtgcc cgctccatcg tgcgcacgag atacgcggac
//
LOCUS       DASRMJ010000178         4068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_520714, whole genome shotgun sequence.
ACCESSION   DASRMJ010000178 DASRMJ010000000
VERSION     DASRMJ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520714"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFD74_04920"
     CDS             complement(<1..488)
                     /locus_tag="VFD74_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HZK48924.1"
                     /translation="MERSKDRATLYGLETAAREGIDTIWDRYEAQLPQCGFGSLGVCC
                     RMCLQGPCRIDPFGEGATKGICGATADTIVARNLARFIAGGTAAHSGHAKHLAHTMLK
                     MADGKAPDYSIRDEAKLKAVAARVGVAIDGRPITDIARDVARAGLLEFSEREEPLAWL
                     ATT"
     gene            complement(657..1466)
                     /locus_tag="VFD74_04925"
     CDS             complement(657..1466)
                     /locus_tag="VFD74_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZK48925.1"
                     /translation="MSHLRREIVKLLFQRRTYVGWLGLLAIPVIMTIALRLSSEQPPE
                     GHGPPFFSNIAGNGLFVPLAALAFLSFFLLPLVSAMTGGFVIAGEAETGTLKTLLTRP
                     VTRGRVLLAKWFVAVLYVGVGLALVGVGGAVAGGLIFGFHPLTTLSGGQLGMAQSLGT
                     IALAYLIVLAGMTCIVSLAVALSTLTDSSLTAAVGTLVIVLILEALGQFSYFDFLKPY
                     LFTSHNEDWFNLLRQPVSWRPIWEALLTYAAYIVVLVGVAVTVFRRKDILS"
     gene            complement(1463..2497)
                     /locus_tag="VFD74_04930"
     CDS             complement(1463..2497)
                     /locus_tag="VFD74_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48926.1"
                     /translation="MTMTDREPLPAPEACAGRRVVLSTHQLTKDFKSLRAVDHLDLSV
                     CEGDIFGFLGPNGAGKTTTIRMILGLIYPTEGHVQILEHRVPDQRLDALRHVGGFVDV
                     PAFYKNMSARRNLRLLGRMSGEVSEARIDEVLDMVGLSQRGDSKVGSYSHGMGQRLGI
                     AQALIHRPALIILDEPTSGLDPQGMKDVRELVRELGRQGTTVFLSSHLLHEVEQVCNR
                     AVIMDKGRIVVQGPVSELRPARMSVKVLTNDRPRTAEVLRRLLGPDAVLNGGDEVHAG
                     EEFLRFMCGDDLVPEIVRRLVAEGIDVRAVVPAVEQGLEDFFLELTGASSKADRGHPD
                     SSRESTGAEQ"
     gene            complement(2502..3923)
                     /locus_tag="VFD74_04935"
     CDS             complement(2502..3923)
                     /locus_tag="VFD74_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48927.1"
                     /translation="MHRKMFFITGVVVALVAALTVGLTVARSQEGTALSALTPVELLA
                     KVAQEAPKTTAVSGDIAWTNDLLGAAALQMPAGDAGLASLMQGGSGRFWYQEGKLRFE
                     SQGSGGDTVAVLNGTTVWVYSSGSGTATEYTLPARPTGGTDSEASNSTDTTEDAGVAA
                     TGDVTAALDLPRKIQDLVDSLAPRATLSVATAEVAGRASYVLTMTPTAPNTIVGSAQV
                     AFDGKTFLPLRVQVFAKGDAQPVLEAGFTKVSYAKVSADTFEFTPPADAKVEHSTLAM
                     PAGMLGVGGDGSSDGGGAASPGESAKHAELTLDEAAVRAGFALAVPSDVALPFQGAAV
                     IDTSTLGGFMMSAFSGSAMGGTAVEAPAPAGGAGISGVTAPAGVTVPDAPLVILRYGA
                     GFGTVVVIETQIDDALWAQMTTALTQVPMLGAPSAFGGHQAYELGTRLGSLVAWRQGD
                     VVVLMGGSVSQADLEAFASSIHE"
BASE COUNT          718 a         1382 c         1333 g          635 t
ORIGIN      
        1 gtggtcgcga gccaggccag cggttcttcc cgctcggaga actcgaggag accggcgcgg
       61 gctacgtcgc gggcgatatc ggtgatcggc cgcccgtcga tagcgacacc tacgcgcgcg
      121 gccacggctt tgagcttggc ctcgtcgcgg atggagtagt cgggcgcctt gccatcggcc
      181 atcttgagca tggtgtgggc taggtgtttg gcgtgaccgg agtgggcggc ggtgccgccg
      241 gcgatgaaac gggccaggtt gcgggcaacg atagtgtctg cggtagcgcc gcatatgccc
      301 ttggtggcgc cttcaccgaa agggtcgatg cggcacggac cctgcagaca catccggcag
      361 cagactccca acgaaccgaa gccgcactgg ggtagctggg cctcgtaccg gtcccagata
      421 gtgtcgatgc cctctcgggc cgccgtctcc aggccgtaga gggtggctcg atccttcgat
      481 cgctccatgt gcggtctcct cccgtttcgc tcgggtgcag ccgcggcgac acccgcttgc
      541 ctgactatcc gaccgctgcc cattcttgca ccgcgaggca aagggcgcaa cgtggtgcga
      601 cgaccccagg aagcgggatc accgggcgcg gcgaatccga acccggcgaa gcgcgctcag
      661 gacaagatgt ccttgcgtcg gaagacggtc accgccaccc ccaccaacac cacgatgtag
      721 gcggcgtagg tgagcagcgc ctcccagatg ggccgccacg agacgggctg gcgcagcaga
      781 ttgaaccagt cctcgttatg ggacgtgaag aggtagggct tgagaaagtc gaagtagctg
      841 aactggccca gggcctctag gatcagcacg atcaccaacg tgcccaccgc ggccgtcaga
      901 ctggagtcgg tgagggtgga gagagccacc gcgagagaga cgatacaggt catgcccgcc
      961 agcacgatca ggtaggccag ggcgatggtc ccgagcgact gagccatgcc gagctgcccc
     1021 cccgagagcg tggtgagcgg gtggaagccg aagatcagcc caccggccac ggcccctccc
     1081 acacccacga gcgccaggcc cacgccgacg tagagcacag ccacgaacca cttggcgagc
     1141 agcacgcgcc cgcgggtgac cggacgggtg agaagtgtct tcaacgtgcc ggtctcggcc
     1201 tcgccggcga ttacgaaacc gccggtcatc gcggagacca gaggcagcag gaagaacgac
     1261 aagaaggcca gggcggccag aggcacgaag agcccgttgc ccgcgatgtt cgagaagaag
     1321 ggcggtccgt gaccctccgg gggttgctcg gaggatagcc gaagggcgat ggtcataatt
     1381 acggggatgg ccagcaggcc cagccaaccc acatacgtgc gcctctggaa gaggagcttg
     1441 acgatctcgc ggcgtaggtg gctcattgtt ccgctcccgt ggactcgcga ctcgagtccg
     1501 ggtggccccg atccgccttg gacgacgccc cggtcaactc gaggaagaag tcctccagac
     1561 cctgctccac ggccggcacc accgcgcgca cgtcgatgcc ctcggcgacc agtcgacgca
     1621 ctatctcggg caccaggtcg tccccgcaca tgaagcgcag gaactcctcg ccggcgtgga
     1681 cctcgtctcc cccattgaga acggcatcgg ggccaaggag gcggcgaagc acctccgccg
     1741 tccgcggtcg atcgttggtg agcaccttca ccgacatgcg cgccggccgc aactccgaga
     1801 ccggaccctg cacgacgatg cgccccttgt ccatgatgac ggcccggttg cacacctgtt
     1861 cgacttcgtg cagcaggtgc gacgagagga acaccgtcgt gccctgccga cccagttcgc
     1921 gcaccagctc gcgcacgtcc ttcatccctt gagggtcgag accgctggtg ggctcgtcca
     1981 ggatgatgag ggcaggccgg tggatcagcg cctgggcgat accaaggcgc tggcccatcc
     2041 cgtgagagta gctccccacc ttggagtcgc ctcgctgcga aagccccacc atgtcgagca
     2101 cctcgtcgat gcgcgcctcg ctcacttcgc cactcatgcg gcccagcagg cgcagattcc
     2161 gtcgcgcgct catgttcttg tagaaggcgg ggacgtccac gaagccgccc acgtgccgca
     2221 gggcgtccag ccgctgatcg ggcacgcggt gctccaagat ctggacgtga ccctcggtgg
     2281 gatagatcaa tcccaggatc atgcggatgg tggtggtctt ccccgcgccg ttggggccga
     2341 ggaagccgaa gatgtcgccc tcgcagacgc tcaggtcgag gtgatccacc gcacgtaggc
     2401 tcttgaaatc tttggtgagc tggtgggtgg agagaactac gcgccggccg gcgcaggctt
     2461 ccggcgccgg caggggttca cgatccgtca tggtcatgac ctcactcatg gatggaggag
     2521 gcgaacgcct ccagatcagc ctgcgagacg ctccctccca tcaacaccac cacgtcgccc
     2581 tgtcgccaag ccaccagcga accgagcctt gtgcccagct cgtatgcctg atgaccgccg
     2641 aacgcgctcg gcgcgccaag catcggcacc tgagtcaggg ccgtggtcat ctgagcccag
     2701 agggcgtcat caatctgggt ctcgatgact acgacggtgc cgaagcctgc gccgtatctg
     2761 agtatcacca gaggcgcgtc ggggacggtg accccggcag gcgcggtgac cccggagatc
     2821 ccggcgcccc cagcaggtgc gggagcctcg accgccgtac cgcccatcgc gctccccgag
     2881 aaggcggaca tcatgaaccc gcccagcgtc gaagtatcga tcacagctgc gccttggaag
     2941 ggcagggcga catcggacgg cacggccaga gcgaagccgg cacgaaccgc agcttcgtcg
     3001 agggtgagct cggcgtgctt ggcggactcg cccgggctcg ccgcgcctcc gccgtcgctg
     3061 ctaccgtcac cgccgacgcc cagcatcccg gcgggcatgg ccagagtcga gtgttcaacc
     3121 ttggcgtcgg ccggaggcgt gaattcgaac gtgtccgccg acaccttggc gtacgagacc
     3181 ttggtgaatc ccgcttcgag cacgggctgc gcgtcgccct tcgcgaacac ctgcacccgc
     3241 agaggcagga aggtcttgcc gtcgaaagcc acctgcgccg acccgacaat ggtgttcgga
     3301 gcggtgggcg tcatagtcag aacgtagctc gcgcggcccg ccacctcggc ggtggcgacg
     3361 ctcagcgtgg cgcgcggcgc cagcgagtcg accaggtcct ggatcttccg aggtagatcg
     3421 agcgcggcgg tcacgtcacc ggtcgcggcg acccctgcgt cttcggtcgt gtcggtggag
     3481 ttcgacgcct cggagtccgt gccccccgtc ggcctagcgg gcagcgtgta ctccgtggca
     3541 gttcccgacc ccgagctgta gacccacacc gtggtgccgt tcaggacggc caccgtgtcg
     3601 ccacccgagc cttgggactc gaaccgcaac tttccttctt gataccagaa tcttcccgac
     3661 ccaccttgca tgagcgaggc cagcccggcg tcgcccgcgg gcatttgcag cgccgcggcg
     3721 cccaacagat cgttggtcca ggcgatgtcg ccactcacgg ccgtcgtctt gggcgcctct
     3781 tgggccacct tggccagcag ctcgaccggc gtgagcgccg agagtgccgt gccctcctgg
     3841 ctgcgggcca ccgtcaagcc caccgttaga gccgcgacca gcgccacgac cactccggtg
     3901 atgaagaaca tcttgcgatg catgaccgac cccttccacg atgcttccga ggactcgagg
     3961 cgctcggagc gtccccttat gcccgctcca gatgcttcag atgccaaccc tatccagctt
     4021 ccgacgctgt aggaagtcta ctccggagtt gtgagcgatg tgtaaggt
//
LOCUS       DASRMJ010000179         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_339515, whole genome shotgun sequence.
ACCESSION   DASRMJ010000179 DASRMJ010000000
VERSION     DASRMJ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339515"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFD74_04940"
     CDS             <1..858
                     /locus_tag="VFD74_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48928.1"
                     /translation="LVTLCAQCAGDEIADQKQVASSRSADEQDATSASRARSLIMKMI
                     DRATQEHSRLHELDALVGDLASRLAAAQLTALEQEERARGLAAELDRTRERLRRAEEL
                     LALANDRPAPAAGAEGGSPHGPAAAPQVHPGLSELTPADVNAIQRAFNDSTFIEKMRS
                     VRRGLGKPIVNACSLAGPDRRALLTVAWDIVWYQFLFDLSDKPEAPGAALFAEGMELS
                     EIADHFKQSNAMIDDQGRLDASELALSLERDHPANEEALDEAEAQALDDATEEIWDST
                     SMPEFRWED"
     gene            1171..1875
                     /locus_tag="VFD74_04945"
     CDS             1171..1875
                     /locus_tag="VFD74_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK48929.1"
                     /translation="MYEVRPDILEVAKALGEETRFNIFRRIADAAEPLSVKDLVAELG
                     MHHSAIRIHLGKLEDAGLIYSKKRHIPGTVGRPQLAFLPSARALSITLPPRNYEFLAR
                     LAMDWAAAEGDTEPTEDFGLRWGRDYVKGRGRLVDGSLPLDRALGVLMDELRRLGASA
                     RSGVMNGGYQVLETNCVFAELAADYEPLVCELHQAVMRGMLSAISADEFEWQQTTRIC
                     DGDERCTMRIAARPPA"
     gene            complement(1898..3964)
                     /locus_tag="VFD74_04950"
     CDS             complement(1898..3964)
                     /locus_tag="VFD74_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK48930.1"
                     /translation="MDSENVPGDGREILALLSDRFSGPDAEAVKARFSRPDMLDVAAV
                     VSWRGAVLTPGVCPVSMALVYLDLVQEESCGRCSPCRIGTHIMREILRRLERGEGRPH
                     DLDTLRLLAEEVDEASWCGIANTVQDPILSLLDLAAEHFEAHLGGQTCDRPKTVGWVT
                     APCRSTCPSTVDCPSYIFQAMEEHPHLATDIVKRDNPLPAVIGRTCHHPCESNCTLAG
                     TGEPVAINFVKRWCADRAEGLATHVPAPGVCGGEASSTSAEPGARFSCCDSPHESTEE
                     STAQSSQRPISAHAGSRGDRVAVIGAGPSGLSAAYYLARRGYRPVIFEALPVPGGMLY
                     VGIPEYRLPKAALRREVALIEQEGVEIRYNAALGRDLAFSDLDELGFSATFIGIGAHS
                     GKKLRIPGEDLPGSMDAIDFLREVALGERTSVGEKVLVIGGGNSAMDAARTALRLGAK
                     EVSVVYRRAREQMPANPWEIDEAMEEGVGFHFLSAPVRCDGDTCVQVLICQEMELGPP
                     DESGRRSPVPSAKAPYGLEADMIIAAVGQQSDFSPFVGDAAIGVNKWGYLEMDPLTFA
                     TTKPGVFVGGDAISGGASVIEAIHAGKTAAKYIDLHLRGLPVVEDIEDKIRRIAVQMG
                     AQSSRYALAPGRDYGRREHMPMLDPEVRNSNFAHCETGYGDAGAKREADRCLRCHRPL
                     VVAVAG"
     gene            complement(4023..4616)
                     /locus_tag="VFD74_04955"
     CDS             complement(4023..4616)
                     /locus_tag="VFD74_04955"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZK48931.1"
                     /translation="MDPSPEVILQAEAAWGIPVEVSVDQPLLAWELDLIERVCGDRRH
                     HDVTIFVRKGDSLAVVRKPSDPPDVWWVPAGGVDPGESLGGAAVREVWEEAGVRSRVE
                     RYLIRMTAVFHCGDRRRCWTSHVIGAAWESADPHPVDTHEVEAARWVSGVEFAEQIVP
                     RMIASGWGRLEYRVRMAALASGLLGLPGSIGLPPHLQ"
     gene            complement(4617..>5254)
                     /locus_tag="VFD74_04960"
     CDS             complement(4617..>5254)
                     /locus_tag="VFD74_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48932.1"
                     /translation="PVGVTLPRGALRRLPFVVMVVGVAAVVAGVALVPPDKVLGAGVR
                     MVFFHGAVTWTGILMTILAGFSGLGLLVFSRPGASVVWRAQSLGAFFWTASLIVSFPV
                     MIRTWGGVMWDEPKLLMSAEIVTGLLVVWALGLIMGTPRVIAGLAVAAATVMGILLSV
                     TPGAFHPDNPILRSGDPLFIGSFFLVAAGLVVTAVGGLSIGRSRERGRERT"
BASE COUNT          913 a         1859 c         1693 g          789 t
ORIGIN      
        1 ctcgtgaccc tctgcgcaca gtgcgccggg gacgagatcg cggaccagaa gcaggtggct
       61 tcgtctcggt cggctgacga acaggatgcc acctcggcga gtcgcgcacg ttccctgatc
      121 atgaagatga tcgatcgggc cacgcaggaa cactcgcgcc tccatgagct cgacgccctc
      181 gtcggcgacc tcgcatcgcg actcgcggcc gcgcaactca cggcactcga gcaggaggag
      241 cgcgcccgcg gccttgctgc cgaactcgac aggacccgcg agagactgcg ccgtgccgag
      301 gagcttctcg cgctggcgaa cgaccgtccc gcgcctgccg cgggcgccga aggcggaagt
      361 ccacacggcc cggcagccgc gccccaggtg caccccggcc tctccgagct gacgccggcc
      421 gatgtgaacg ccatccagcg cgcgttcaac gactcgacgt tcattgagaa gatgcgcagc
      481 gtccggcgcg ggctgggcaa gccgatcgtc aacgcgtgca gccttgcggg gcccgaccgg
      541 cgtgctctcc tcacggtggc gtgggacatc gtctggtacc agttcttgtt cgacctctcc
      601 gacaaaccgg aggccccagg tgccgccttg ttcgccgagg gcatggaact ctccgagatc
      661 gccgatcact tcaagcagtc caacgccatg atcgacgacc agggccgcct cgacgcgtcg
      721 gagctcgctc tttcccttga gcgcgaccac ccggccaacg aggaggcact ggacgaggcc
      781 gaggcgcagg ccctcgacga cgccaccgag gagatctggg acagcacctc gatgcccgag
      841 ttccgctggg aagactgagg ccctcgcccg cctcgcggtg ctcccgcact gaggcggcgg
      901 gcacccgcgc gcctcgcggc ctccgcccta tgcgcgcctc gtgccgggac tcaggccgcc
      961 ctcgcggcgc ccctgtgccg gctccagccc gccctcgcgg cgcctctgtg tcgcctccgg
     1021 gccaccgtcg ccgcctccgg caaggtcttt gctgcgggcc ggtgagagca ccggctgatg
     1081 aggtttggcg gtgggacggc gctgtggtac ggttcggagc gaggtccgtc aagtcactct
     1141 ggggccgaca aactcgccga acgcggagtc atgtacgagg tccgaccaga tatcctcgaa
     1201 gtagcgaaag cgctcggaga agagactcgg ttcaacatct tccgccgcat cgcggacgcc
     1261 gccgagcctc tatccgtcaa ggaccttgtc gctgaactcg ggatgcacca cagcgcgatc
     1321 cgcatccacc tgggcaagtt ggaggacgcg gggctcatct actcgaagaa gcgtcacatc
     1381 ccggggaccg tgggccgccc tcagttggcg ttcctgccca gcgcgcgggc cctctcgatc
     1441 actctgccgc cccgcaacta cgagttcctg gcccgcctcg ccatggactg ggccgcggcg
     1501 gagggggaca cggagccgac cgaggatttc ggtttgcgct gggggcgcga ctacgtgaag
     1561 ggccgcgggc gcctggtcga cggttccctg ccgctcgatc gtgccctcgg cgtgctcatg
     1621 gacgaactcc gccgcctcgg agcatcggcc cgctcgggcg tcatgaacgg cggctaccag
     1681 gtgctcgaga ccaactgcgt gttcgcggaa ctggccgccg actacgagcc gttggtctgc
     1741 gaactccacc aggcggtgat gaggggcatg ttgtccgcga tctccgccga cgagttcgag
     1801 tggcagcaga ccaccaggat ctgcgacggg gacgaacgct gcaccatgcg gatcgcggcc
     1861 cgtcccccgg cctgaccccg ctctcaacgg acgtgaccta ccccgcgacg gccaccacaa
     1921 ggggccgatg acaccgtagg caccggtctg cttcgcgttt ggccccggca tcgccgtagc
     1981 ccgtttcaca gtgcgcgaag ttgctgttgc gcacctcggg atccagcatg ggcatgtgct
     2041 cacgtcggcc gtagtcgcgg cccggcgcga gtgcgtaccg actcgactgc gcccccatct
     2101 gcaccgcgat gcgcctgatc ttgtcctcga tgtcctcgac gacgggcagt cctcgcaggt
     2161 gaaggtcgat gtacttggca gccgtcttgc cggcgtggat ggcctcgatg accgaagcgc
     2221 ccccgctgat cgcgtcccca cccacgaaca cgcccggctt ggtggtcgcg aacgtcagtg
     2281 gatccatctc caagtagccc cacttgttga cgccgatggc cgcgtccccg acgaacgggc
     2341 tgaagtcgga ttgctggccg acggctgcga tgatcatgtc ggcctcgagt ccgtagggag
     2401 cctttgcgct gggcaccggc gagcgtctgc cgctctcgtc cggcgggccg agttccatct
     2461 cctggcatat caggacctgc acacacgtgt ccccgtcgca gcgcaccggg gccgagagga
     2521 agtggaagcc taccccctct tccatggcct cgtcgatctc ccaggggttc gcgggcatct
     2581 gctcgcgcgc gcgccggtag acgacgctca cctccttggc tcccagacgc agcgccgtgc
     2641 gcgccgcatc catcgctgag ttgccgcctc cgatcaccaa caccttctct cccaccgacg
     2701 tgcgctcgcc aagagcgact tcgcggagga agtcgatcgc gtccatcgac ccgggcaggt
     2761 cctcgccggg gatgcgcagt ttcttgcccg agtgagcgcc gatacctatg aacgtggcgg
     2821 agaatcccaa ctcgtcgagg tcggagaagg caaggtcacg gcccagagcg gcgttgtagc
     2881 ggatctcgac gccttcctgc tcgatcaggg cgacctcgcg ccgcagtgcc gccttgggca
     2941 gacggtattc ggggatgccc acatagagca tcccgccggg caccggcaga gcctcgaaga
     3001 tgaccggcct gtagcctcgc cgtgccaggt agtaggcggc ggagagaccg gatgggcccg
     3061 ccccgatcac ggccacacgg tcgccacggg agcctgcgtg ggccgagatg ggtcgctggc
     3121 tggactgagc ggtcgactcc tcggtcgact cgtgcggtga atcgcaacag gagaagcgcg
     3181 cgccgggctc ggcggaggtc gaagaagcct ccccgccaca gaccccggga gccggcacgt
     3241 gcgtcgccaa gccctccgcc cgatccgcgc accatcgctt cacgaagttg atggccaccg
     3301 gttcaccagt gccggccaag gtgcagttcg attcgcaggg atggtgacac gtgcggccga
     3361 tgaccgcggg cagaggattg tcacgcttga cgatgtcggt ggccagatga ggatgctctt
     3421 ccatcgcttg gaagatgtag ctcggacagt cgacggtcga aggacaggta gaccggcagg
     3481 gcgcggtgac ccaaccgacc gtcttcggcc tgtcgcacgt ctgtcccccc aggtgggcct
     3541 caaagtgctc ggcggcaaga tcgagcagcg agagaatcgg gtcctgcacg gtgttcgcga
     3601 tgccgcacca cgacgcctcg tccacttcct ccgccaggag acgcagggtg tcgaggtcgt
     3661 gcggccgacc ttcgccgcgc tcgagtcggc ggaggatctc gcgcatgatg tgggtgccga
     3721 tgcggcatgg actgcagcgc ccgcacgact cttcttggac gaggtccagg tagaccagag
     3781 ccatcgatac gggacacacg cccggggtca gcacggctcc ccgccaggac accacggctg
     3841 ccacgtcaag catgtcgggc ctggagaagc gggccttgac cgcttcggcg tcgggccccg
     3901 agaagcgatc actcaacaga gccaggatct cccgaccgtc accaggcacg ttctcgctat
     3961 ccatttctcc tcctcacgca accgcgacga gagatcccag ggaggaccgt tcaccgtcct
     4021 attcattgta gatgaggagg gagcccgatg ctaccgggca ggccgagcag gcccgaagcg
     4081 agcgccgcca tcctcacgcg atactccaaa cgcccccaac ccgacgcaat catgcgcggc
     4141 acgatctgtt cggcgaactc gacgcccgac acccaacggg ccgcttccac ctcgtgcgtg
     4201 tcgaccgggt gcgggtcggc actctcccag gcggctccga tgacgtgcga cgtccagcac
     4261 cggcggcgat ctccgcagtg gaacactgcc gtcatgcgga tcagatagcg ctccacccga
     4321 gagcggacgc cggcctcctc ccacacctcc cgcacggccg caccgccgag actctcaccg
     4381 gggtccactc cgccggccgg gacccaccac acgtcgggag ggtcggatgg cttccgcacc
     4441 acagcgagcg agtcgccctt gcgcacaaag attgtgacat cgtggtgccg acggtcgccg
     4501 cacacgcgtt cgatgagatc gagttcccac gccagcagag gttgatccac cgaaacctcc
     4561 accggtatcc cccacgcggc ctcagcctgc aggatcacct caggggaggg gtccacctag
     4621 gtgcgctcgc gcccgcgctc gcggcttctg ccgatcgaaa gccctccgac ggcggtaaca
     4681 accaggcccg cggcaacaag aaagaagctg ccgatgaaca acgggtcgcc ggacctcagg
     4741 atgggattgt cgggatggaa agccccgggc gtcactgaga gcaagatccc catgaccgtg
     4801 gcggccgcga cagccaggcc ggcgatcacc cgtggggtgc ccatgatgag gcccaaggcc
     4861 cagacgacga gcagtcccgt cacgatctcc gcggacatca gcaacttggg ttcgtcccac
     4921 atgaccccgc cccacgtcct gatcatgacg ggaaagctga caatcagaga agcggtccag
     4981 aagaaggccc cgagggattg cgccctccac acgacggagg cgcccggtcg agagaagacg
     5041 aggaggccca gccccgagaa gccggccaga atggtcatca agatgccggt ccacgtcacg
     5101 gctccatgga agaagaccat gcgcacgccg gcgccaagca ccttgtccgg agggacgagc
     5161 gccacgcccg ccaccacggc ggccacgccg acgaccatca ccacgaacgg cagacgtctt
     5221 agagcccctc tggggagcgt cacccccact ggag
//
LOCUS       DASRMJ010000180         4293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_93897, whole genome shotgun sequence.
ACCESSION   DASRMJ010000180 DASRMJ010000000
VERSION     DASRMJ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93897"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3923
                     /locus_tag="VFD74_04965"
     CDS             <1..3923
                     /locus_tag="VFD74_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HZK48933.1"
                     /translation="LSTEPNLGVWKLRATAVVGVSGDGAAASAGDASTELDVRVEEYV
                     LPKYEVSVETAKDWFLVSEPITGHVTAAYSFGRPVRGELRITASRYVGEWQEFATFTT
                     DIDGEADFRLDPAGYVAGVPEAGGQGNVTLEVAVVEPATGYEEKTTQLLTVAAAPVTM
                     KLIGESPSFKPGLPFDLLLVTETPGGEPVEAAVDVQVYYYSENFETVGQDTRRVETKR
                     GTALLDLTPPKGAVRMELNAGSGDAYTYMQSTAAYSPSGNFIHLTQEDAGTLSVGDTA
                     RFRVASTSEARTCYYEVVARDRVVFSSSEGASGGGSNEIAFKVTPAMAPGAKLLVYQI
                     LPTSEVAADSLPFEVTGVYPHTVSATFDVQEARPGDAVEVAVQTEGPAKVGLVAVDHS
                     VFILAENRLNLQQVFAELERLYMQPQAELHEADPMMGLPLLSPGAKDTFQDAGLIVLS
                     DKDVPEGKEMEDPRGFVGLGGGFMENAADGVLPLRAQSPPTTAASAVGDKTQTSATGG
                     LAEVQRVRQFFPETWIWDEVVTDRNGKATLKVDAPDSITTWDLRAVALSPDKGLGVAE
                     SSLRVFQPFFLTADLPYSAIRGEELPLKVSLYNYLDTPQEFQVQIEDSDWFDMLDDAT
                     TTVTVAANDIGGAEFTIRPKTLGTQLVKVTARGPQAADAVIKSMLVEPEGVARELVEN
                     IVLQTGAEHTIDLRPLVALPLEGGGSAGTGGSSTSTTGSGGSSTGTDVRPGMPFPAWP
                     EVVPDSSRAYVAISGSLLAQTIDGLDQLLQMPFGCGEQNMILFAPDAFILRYLKETRQ
                     LKPEIQAKAEMLLVTGYQRELTYQRADGSFSAFGDQDPEGSLFLTAFVLKTFAQAQDL
                     TYIDPQVLSDASAWIESHQKADGSFEPVGFVAHQEMVGGIQGADALTAYATVALLEAD
                     RAAPAGSAAAGRSAADKALGYLEGRLHAIDDPYTLALTTYALELGASAKADAAYDKLM
                     KAAIVDDEGLHWSGGGVEPLPGGTGGGIGGGGGDQPVAQPAAQPAGGDQAAMPPIDIY
                     DPSFRPPLDIEATVYATLALIEHGDRLSAGKAAKWLVARRNSAGGFGSTQDTVVALQA
                     LTEYAVFGAVDTDLTVTLRAGDVDKQVRITPDNYDVTQVIEVPAGVPVQLRAAGKGEA
                     VVQGVLRYNIREPEPVQSVFDISVDYDADQVAVNDLVDVDVSLAFNPPVLLDTGTEAY
                     PARPVKAGMVVLDVSVPTGFAPEAATLESLLQRPKIKRYDVAGRKVIIYIEDMTAGEK
                     ISFSFQVRALYPVRAKGGASQAYSYYTPQWKGETMGEAIVVRGG"
     gene            complement(4071..>4293)
                     /locus_tag="VFD74_04970"
     CDS             complement(4071..>4293)
                     /locus_tag="VFD74_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48934.1"
                     /translation="YENGEEHSFMSVMKKHGLTSLQMQQLSDELRDIYSAHKDQPRYT
                     YPLQRQELVVTPDESLARDVERVIGEIGS"
BASE COUNT          720 a         1517 c         1390 g          666 t
ORIGIN      
        1 ccctctccac cgagcccaac ctgggggtgt ggaagctccg tgccaccgcg gtcgtcggcg
       61 tctccgggga cggcgccgcc gcatcggcgg gcgacgcctc caccgagctc gacgtacgcg
      121 tggaagagta cgtcctgcca aagtacgagg tctccgtcga aacggccaag gactggttcc
      181 tggttagcga gcccatcacc gggcacgtca ccgccgccta cagcttcggg cgtccggtgc
      241 gcggggagct gcgcatcacc gcctcccgct acgtgggcga gtggcaggag ttcgccacct
      301 tcaccaccga catcgacggc gaggccgact tccgactcga tcccgcgggc tacgtggccg
      361 gcgtgcccga ggccggaggc caaggcaacg tgacgctgga ggtggccgtg gtggagccgg
      421 ccaccggcta cgaggagaag accacccagc tcctgacggt ggcggcggcc cccgtcacca
      481 tgaagctcat cggcgagagc ccctccttca agccgggcct gcccttcgac ctccttctgg
      541 tgaccgagac ccccggcggc gaaccggtgg aggccgcggt cgacgtgcag gtgtactact
      601 acagtgagaa cttcgagacg gtgggtcagg atacccgccg cgtcgagacg aagagaggca
      661 ctgccctgct cgatctcacg ccgccgaagg gcgccgtgcg catggagttg aacgccggct
      721 ccggtgacgc ctacacctac atgcagtcga ccgccgccta ctcaccctcg ggcaacttca
      781 tccacctcac ccaggaagac gcgggaaccc ttagcgtggg cgacacggcc cgcttccgcg
      841 tggcctccac ctctgaggcc cgcacctgct actacgaagt cgtggcccgt gatcgcgtgg
      901 tcttcagcag cagcgagggt gcctccggcg gcggttccaa cgagatcgcc ttcaaggtga
      961 ccccggccat ggcgccgggg gccaagctgc tcgtgtacca gatcctgccc accagcgagg
     1021 tggcggccga ctccctgccc ttcgaggtga cgggtgtcta tccgcacacg gtgagcgcca
     1081 ccttcgacgt gcaggaagcc aggcccggag acgcggtgga agtagctgtg cagaccgaag
     1141 gtccggccaa agtggggctc gtggcggtgg atcactccgt gttcatcctg gccgagaacc
     1201 gactcaacct gcagcaggtc ttcgccgaac tcgagcgcct ctacatgcaa ccgcaggccg
     1261 aactccacga ggccgacccc atgatgggcc tgccgctcct ctctcccggg gccaaggaca
     1321 ccttccagga cgccggcctc atcgtgctct ccgacaagga cgttcccgag ggcaaggaga
     1381 tggaggaccc ccgaggcttt gtcggtttgg gcggcggctt catggagaac gccgccgatg
     1441 gcgttctccc gcttcgcgcc cagagccccc ccaccacggc tgcttctgcc gtcggcgaca
     1501 aaacccagac ctcggcgacc ggcggattgg ccgaagtgca gcgtgtgcgt cagttcttcc
     1561 ccgagacgtg gatatgggac gaggtggtca ccgaccgcaa cggcaaagcc accctgaagg
     1621 tggacgctcc cgactccatc accacctggg acctgcgtgc cgtagcgctc tcgcccgaca
     1681 aggggctggg agtggccgag tcgtcactga gggtattcca gccgttcttc ctcacggccg
     1741 acctgcccta ctccgcgatc cgaggggagg agctgccact gaaggtcagc ctctacaact
     1801 acctcgacac gccgcaggag ttccaggtgc agatcgagga ttcggattgg ttcgacatgc
     1861 tcgatgacgc caccactacg gtcaccgttg ccgccaacga catcggcggc gccgagttca
     1921 cgatccgacc caagaccttg ggcacgcaac tggtcaaggt gaccgcccgc ggtccgcagg
     1981 ccgccgacgc cgtcatcaag agcatgctcg tcgagcccga gggcgtggcc cgcgaactcg
     2041 tggagaacat tgtgctgcaa accggcgccg agcacaccat cgatctccgg ccgctggtgg
     2101 cgctgccgct cgaaggcggc ggttcggcgg gcaccggcgg cagctccacc agcaccaccg
     2161 gctcgggcgg cagctccacc ggcaccgacg tcaggccggg catgccgttc ccagcgtggc
     2221 ccgaagtggt ccccgactcc agccgcgcct acgtggccat cagcggcagt ctcctggccc
     2281 agaccatcga cggactcgac cagctcctgc agatgccctt cggctgcggc gagcagaaca
     2341 tgatcctctt cgcgcccgat gccttcatcc tgcgctacct gaaggagaca cggcagctca
     2401 agcccgagat ccaggccaag gccgagatgt tgctggtgac cggctaccaa cgcgaactca
     2461 cctaccagcg cgccgacggc agcttctccg ccttcggcga ccaagatccc gagggcagcc
     2521 tcttcctcac cgccttcgtg ctcaagacgt tcgcccaggc gcaggacctc acctacatcg
     2581 acccgcaggt gttgtccgat gcgtccgcgt ggatcgagag ccaccagaag gccgacggtt
     2641 cgttcgagcc ggtgggcttc gtggcccacc aggagatggt gggcggcatc cagggagctg
     2701 acgccctcac cgcctacgcg acggtcgccc tgctcgaagc ggaccgcgcc gcgccggccg
     2761 gatccgccgc ggccgggcgg tccgccgctg acaaggccct cggttacctc gaagggcgtc
     2821 tccacgccat cgacgacccc tacactctgg ccctgaccac ctatgccctg gagctgggcg
     2881 cgagcgccaa agccgacgcc gcctacgata agctgatgaa ggctgccatc gtcgacgacg
     2941 agggcctcca ctggagcggc ggcggcgtcg aacccctgcc gggcggcacc ggcggcggaa
     3001 tcggcggcgg tggcggcgac cagcccgtcg cgcagcctgc cgcgcagcct gccggcggag
     3061 accaggcagc catgcctccc atagacatct acgatcccag cttccgcccc ccactcgaca
     3121 tcgaggccac cgtctacgcc accctcgccc tgatcgagca cggtgaccgt ctcagcgccg
     3181 gcaaagcggc caagtggttg gtggcgcggc gcaacagcgc tggcggcttc ggctctacgc
     3241 aggacacggt ggtggctctg caggccctca ccgagtacgc ggtcttcgga gccgtcgaca
     3301 ccgatctcac cgtgaccctg cgtgccggcg acgtcgacaa gcaggtccgc atcacccccg
     3361 acaactacga tgtcacccag gtgatagagg tgcctgcggg cgtgccggtg cagctgcggg
     3421 ccgcgggcaa gggtgaggcg gtggtccagg gcgtgctgcg gtacaacatc cgcgaaccgg
     3481 agccggtgca gagcgtcttc gacatcagcg tggactacga cgccgatcag gtggcggtga
     3541 acgacctggt cgacgtcgac gtgagccttg ccttcaatcc tccggtgttg ttggacacgg
     3601 gcactgaggc ctatcctgcc cgccctgtca aagcgggcat ggtggtgctc gacgtgtctg
     3661 tgcccaccgg cttcgccccc gaggctgcga cactcgagtc cctactccag aggcccaaga
     3721 tcaagcgcta cgacgtggcc gggcgcaagg tcatcatcta catcgaggac atgaccgcgg
     3781 gcgagaagat ctcgttctcg ttccaggtgc gggcgctcta tccggtgcgc gccaagggcg
     3841 gcgcctccca agcctactcc tactatacgc cgcagtggaa gggcgagacc atgggggagg
     3901 ccatagtggt gcggggcggg tgaccgttcg gcccgggtga ccgttcaagg ccttcggccg
     3961 agccgactcg ccgcccgccg ggctgagctg gggtccgcag atcggccgca gcccggtctc
     4021 gtgagcggtg gtcccgcccg cagcgcggcg cgggaccacc gctccttcag ctaggagcct
     4081 atctctccga tcacgcgctc gacgtcacgt gcgagtgatt cgtcgggcgt caccaccagc
     4141 tcctgacgct gcaacgggta tgtgtagcgc ggctggtcct tgtgggccga gtagatgtcg
     4201 cggagttcgt cggagagctg ctgcatctgc agactggtga ggccgtgctt cttcatgacc
     4261 gacatgaagc tgtgctcttc gccgttctcg tac
//
LOCUS       DASRMJ010000181         7142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_49895, whole genome shotgun sequence.
ACCESSION   DASRMJ010000181 DASRMJ010000000
VERSION     DASRMJ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49895"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFD74_04975"
     CDS             complement(<1..437)
                     /locus_tag="VFD74_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48935.1"
                     /translation="MDPEFLSRFEAGLDLRSPAASVIPARVLGHGEITTVLDISRPTA
                     GATTAVACKRMPMFRSDAEAAAYDTLYHEYEQILREEVGVDVVESRLVWLGWDPDAGA
                     GRVDAAPGRPLADAPAPSARSSAADPAPSPLGRAGAVRTIVLY"
     gene            complement(449..1711)
                     /locus_tag="VFD74_04980"
     CDS             complement(449..1711)
                     /locus_tag="VFD74_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK48936.1"
                     /translation="MTDRPAVDRRGIFDLFARHVSPGKVAFFERAGIDFALGRREGPY
                     LWDADGGHRLIDCHCNGGVFNLGHRNPDVLNALSRSLEELDIGNHHLISPRRAELAAK
                     LAASAPGDLPFAVFGVSGGEAVDLAIKVARAYTGRAGIVSAFGGYHGHTGLALAAGDA
                     KYRTPFGPPTPGFSQVEFGDGQALEVALNADTAAVILETVPATLGMPIAAPDYFPRVR
                     ELCDAAGAVLIIDEVQAGLGRTGRLWGIEHYGVEPDIMVIGKGLSGGVYPMTATLLRR
                     ELEQVFHADPFIHISTFGGAEVGCPVALRVLELSSELAFLGHVGDLAEAFAAGFEQLR
                     RRHPGVLVGLRQLGLMMGIELADAALGPALSKTAFDAGVLAVYAYNDPRVVQLLPPLN
                     IDLTLAQEVLSRLDEALSALERIAGARG"
     gene            2179..3540
                     /gene="gdhA"
                     /locus_tag="VFD74_04985"
     CDS             2179..3540
                     /gene="gdhA"
                     /locus_tag="VFD74_04985"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HZK48937.1"
                     /translation="MNDYGASVMAQVKAKNPAEPEFHQAVFEVLESLDVVLERHPEYR
                     AAKIMERLVEPERAIIFRVPWVDDQGQVQVNRGFRVQMNSAIGPYKGGLRFHPSVNLG
                     IMKFLAFEQVFKNSLTTLPMGGSKGGSDFDPKGKSDNEVMRFCQSLMTELFRHIGPDT
                     DVPAGDIGVGGREIGYLFGQYKRLRNEFTGVLTGKGINWGGSLIRPEATGYGTIYFAA
                     EMAAGRGDTLEGKTCLVSGSGNVAQYAAEKLIQLGAKPVTLSDSNGFIYDAAGIDAGK
                     LSFVMDLKNKRRGRIKEYVETYPTAVYTELAAGSDQNPLWNTPADCAFPCATQNEISG
                     IDAGNLLRNGVKLVAEGANMPTHLDGVEQFLDAGILFAPGKASNAGGVATSGLEMSQN
                     SMRLSWSREEVDERLHRIMRDIHQACVTASDKYGLAGNYVAGANIAGFLKVANSMLDQ
                     GVV"
     gene            3707..6691
                     /locus_tag="VFD74_04990"
     CDS             3707..6691
                     /locus_tag="VFD74_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZK48938.1"
                     /translation="MPSVHELLAGLSNRSRFQDFEVLMSSRVENIILVSSLYDKFILQ
                     EDGQLSEVILGEFLDLHLHHTTGLTHVSSGAEAIELACSNPTFNLIITAINVGDMNAV
                     ELANRAREAGLIIPVIVLAYDGGELSDFVVRHDVTALDRLFLWQGDVRILLAIIKYIE
                     DKLNAEHDTQVAGVQVILVVEDSIRSYSSFLPMIFTELIRHSQRLIPGGMNIAHKILR
                     MRARPKILLCTTYEEAWENFTRYQQDVLGIISDIEFPRDGELDPEAGVEFATHVRELW
                     PDVPVVLQSGRPENEALARRIGASFLLKGSATLLHDLRRVMIEEFSFGDFVFRLPDGS
                     EIDRAGDLRTLAAKLHTIPIESMSYHAERNDFSRWLKARTEFALAHRLRPRKIGQFDS
                     AEAMKADLIEAVEEYRREQGRTIIADFDHRSFDTTSSFSRIGGGSLGGKARALAFVRH
                     LLGAFDMFARFPGVQIAVPPSVVLGTDVFDQFLDRNDLRDLAIHSDDDDEIERRFQAA
                     ALPHEVTRDLASFLELIRYPLAVRSSSLLEDSQYQPLAGVYETFMLPNNHPDAGVRLR
                     QLEATIKRVYASTFSSHAKSYLEATLYRLEEEKMAVIIQKVVGATHEERFYPDISGVA
                     RSYNFYPTSPMTGEDGVAIVALGLGRTVVKGERAHSFCPKYPRHQLMAGSLEDTLRNS
                     QREFWAVELGPFANDPDAGEPAPDLWEKKYPLEVAEEDGTLAQLGSTYSPENDALYDG
                     VSRPGVRVVNFAPLLKFDLFPLADIVDLLLEIGAQGMGTPVEIEFAANLTANEGAPKE
                     FAFLQVRPMGFSREMEDLTLEESGSERVLCHSTSILGNGRLNGIKDVVVVDISRFDRS
                     RSLEVAREVARLNARLTREGARYILIGVGRWGSADPWLGIPVNWDEIAGARVIVESEL
                     KDIPVTPSQGSHFFQNLTSFRVGYFTVNPEAGAGRVDWEWLAAQPAASEGEFVRHLRF
                     DHPLVVSMNGTTREGIILKPKE"
     gene            6869..7123
                     /locus_tag="VFD74_04995"
     CDS             6869..7123
                     /locus_tag="VFD74_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZK48939.1"
                     /translation="MNQSHVKLYALSTCGHCRNVKNFLNENGIEYSSVEVDLTTGEER
                     QRIIDEVRALNPNTSFPTIVIGETVIVGFKEAAMREALGL"
BASE COUNT         1303 a         2424 c         2270 g         1145 t
ORIGIN      
        1 atgtagagca cgatagtacg cacggcgccg gcgcgaccga gcggtgaagg cgccgggtcg
       61 gcggccgagg atcgggccga cggcgccggc gcgtcggcca gagggcgccc cggggctgcg
      121 tcgaccctgc cggcgccggc gtccggatcc caccccagcc acacgagccg gctctcgacc
      181 acgtcgacgc ccacctcctc acgaaggatc tgctcatatt cgtggtagag cgtgtcgtac
      241 gccgctgcct cggcgtcgct ccggaacatg ggcatgcgct tacaggccac cgcggtggtg
      301 gcccccgccg tcggacgtga gatgtcgagc acagttgtga tctccccatg ccccaagacc
      361 cgcgcaggaa tcacgctcgc tgccggcgat ctgaggtcga gcccggcctc gaacctgctc
      421 aggaactcag ggtccacgga cactccgctc atccgcgcgc ccccgcgatc cgttccaggg
      481 ccgagagggc ttcgtcgagc cgagagagga cttcctgcgc cagcgtcaag tcgatgttga
      541 ggggcgggag gagctgcaca acccgcgggt cgttgtaggc gtacacggcc aggacaccgg
      601 cgtcgaacgc cgtcttggag agcgcgggac cgagagccgc atcggccagc tcgattccca
      661 tcatcaaccc cagctgtctg aggcccacca acacgccggg gtggcgccgc cgcagctgct
      721 cgaagcctgc cgcgaaggcc tcggccagat caccgacgtg cccaaggaag gccaactcgg
      781 aactcagctc aagcacgcgc agagccacgg gacacccgac ctcggccccg ccgaaggtgg
      841 agatgtggat gaaggggtca gcgtggaaca cctgctcaag ctcgcggcgc agcagggtgg
      901 ccgtcatggg atacacgccg ccggaaaggc cttttccgat caccatgatg tccggctcga
      961 caccgtagtg ttcgatgccc cacaggcggc ccgtgcgacc caaccccgcc tgaacctcgt
     1021 cgatgatgag cacggcgccg gcggcgtcgc agagttcacg aactcgcgga aagtagtccg
     1081 gggcggctat cggcataccc agtgtcgctg gaaccgtctc gagtatgaca gcggcagtgt
     1141 cggcgttgag agccacctcc aaagcctgcc cgtcgccgaa ctcgacctgg ctgaagcccg
     1201 gagtcggggg cccgaagggt gtccgatact tcgcgtcgcc ggcggcgagt gccaaccctg
     1261 tgtgaccgtg atatccgccg aacgccgaca cgatgccggc gcggccggtg taggcccgcg
     1321 ccaccttgat ggccaggtcg acggcttccc cgccggagac gccgaagacg gcgaagggta
     1381 ggtctccggg agccgaggcg gccagcttgg cggcgagctc cgcccgccgc gggctgatca
     1441 ggtgatggtt gccgatgtcc agctcctcga gggagcgcga aagggcgttc agcacatccg
     1501 ggttccggtg cccgaggttg aacaccccgc cgttgcagtg acagtcgatc agacgatgtc
     1561 caccgtcggc gtcccacaag tacgggccct cacgacgccc cagggcgaag tcgatgccgg
     1621 cccgctcgaa gaaggccacc ttcccgggag acacgtgacg ggcgaagaga tcgaagatcc
     1681 cccgtctgtc gaccgcgggc ctgtcagtca tggatgcgaa ccgcgtccgt catggcacct
     1741 ccgtctcctc gatcggggcg atcctgatcg tatgactgta cctgccaagc cgtggccgaa
     1801 gcaagcttct cgcaccgttc ggcgcagcgg gtgggcgatg cgcccgaagt gcacagtgct
     1861 gcacatggag caccttcgtg tgcacatgca tcattgccgc gccgcccggg cgggcggagt
     1921 atcgtctcgc gatcgagacg gacacaccgc gacggggccc cacgccccct cggcgacggg
     1981 cgcctgtcgc gagatgttgg atcttcctac agtggcggac tcccatcccg tgatatacca
     2041 acagtgacgc gccgcgtggg actgcctata gtacctagtg tggtgattgt cgcccgagga
     2101 cgggtgaagt ctcaaccagg cgccgacgag acgactgggt cagcggcgga gaggcacaag
     2161 agtgaacgga ggcgtggtat gaacgactat ggtgcttcgg tgatggcgca ggtgaaggcc
     2221 aagaacccgg ctgagccgga gttccatcag gcggtcttcg aggttctaga gtcgctcgat
     2281 gtggtcctcg aacgtcaccc cgagtaccgc gcggcaaaga tcatggagcg cctcgtggaa
     2341 cccgagcggg ccatcatctt ccgcgtcccc tgggtggacg accagggcca ggtccaggtg
     2401 aaccgaggct tccgcgttca gatgaacagc gccatcggtc cgtacaaggg cggcctccgc
     2461 ttccacccgt cggtcaactt gggcatcatg aagttcctcg ccttcgagca ggtattcaag
     2521 aactccctca ccactctacc catgggcggc agcaagggcg gttctgactt cgacccgaaa
     2581 ggcaagagcg acaacgaggt gatgcgcttc tgccagagcc tcatgaccga actcttccgc
     2641 cacatcggcc cggacaccga cgttcccgcc ggggatatcg gcgtcggtgg ccgcgagatc
     2701 ggctacctct tcggtcagta caagcgcctg cgcaacgaat tcaccggcgt gctcaccggc
     2761 aaggggatca actggggagg ctcgctgatc cgcccggaag ccaccggcta cggcacgatc
     2821 tacttcgccg cggagatggc tgcgggcaga ggcgacactc ttgagggcaa gacctgcctg
     2881 gtctcgggtt cgggcaacgt ggcccagtac gctgcggaga agctgatcca actgggcgcg
     2941 aagcccgtca cgctctccga ctcgaacggc ttcatctacg acgctgccgg catcgacgcg
     3001 gggaagctgt ccttcgtcat ggacctcaag aacaagcggc gcggccgcat caaggagtac
     3061 gtggagacct atcccacggc ggtgtacacg gaactcgccg ccggttccga tcagaacccg
     3121 ctctggaaca ccccggccga ttgcgccttc ccctgcgcca cgcagaacga gatcagcggc
     3181 atcgacgccg gcaacctgtt gcgcaacggt gtcaagttgg tggccgaagg cgccaacatg
     3241 cccacacatc tggacggcgt ggagcagttc ctcgatgcag gcatcctctt tgctcctggc
     3301 aaggcgtcca acgccggcgg agtagcgacg tccggcctcg agatgtcgca gaacagcatg
     3361 cgcctctcgt ggtcgcgcga agaggtggac gagcgcctgc accgcatcat gcgcgacatc
     3421 caccaggcat gcgtcaccgc ctccgacaag tacggcctgg ccggcaacta cgtggccggc
     3481 gcgaacatag cgggcttcct gaaggtggcc aactccatgc ttgaccaggg cgtcgtgtag
     3541 gcggtcccgc ctcacgcacc gcaggatctt cgagaggcgg gcggccggca ggccgcccgc
     3601 ctcttccgct tcagggccga ccgaagggtc tcctgctatc ctgttctcca acgtaccggg
     3661 cgcgcgcccc gcgccgcctc tcgcagccgg agtgatgggg gaaccgatgc cgtctgtcca
     3721 tgagttgctc gccggcctca gcaaccgcag caggttccag gatttcgagg tgctgatgtc
     3781 ctcccgcgtg gagaacatca tcttggtctc gagcctctac gacaagttca tcctgcagga
     3841 ggatgggcag ctcagcgaag tgatcctcgg cgagttcctc gatctgcacc tacaccacac
     3901 cacggggctt acacacgtct catccggcgc cgaggccatc gagttggcat gctccaaccc
     3961 caccttcaac ctcatcatca ccgccatcaa cgtgggcgac atgaacgccg tcgagctcgc
     4021 caacagagcg cgcgaggccg gcctcatcat ccccgtgatc gtgctggcct acgacggtgg
     4081 cgaactctcc gatttcgtgg tgcgtcacga cgtcaccgcg ctcgatcgtc tcttcctctg
     4141 gcaaggcgac gtgaggatcc tcctcgccat catcaagtac atcgaggaca agctcaacgc
     4201 cgagcacgac acgcaggtag ccggggtgca ggtgatcctg gtggtcgagg acagcatcag
     4261 gtcgtactcc tcgttcctcc ccatgatctt cactgagctc atccgccact cgcagcggct
     4321 catccccggt ggcatgaaca tcgcgcacaa gatcctgcgc atgcgggcgc gccccaagat
     4381 cctgctctgc accacctacg aggaagcctg ggagaacttc acccggtatc agcaggacgt
     4441 actcggcatc atctccgaca tcgagttccc gcgggacggc gagttggacc cggaggccgg
     4501 cgtcgaattc gcgacccacg tgcgcgagct ctggcccgac gtgcccgtcg tgctccagtc
     4561 gggccgcccg gagaacgagg cactggcgcg gaggatcgga gcttcgttcc tgctcaaagg
     4621 gtccgccacc ctgctgcacg acctgcgccg cgtcatgatc gaggagttct ctttcggcga
     4681 cttcgtgttc cgactgcccg acggctcgga gatcgaccgg gccggcgacc tgcggactct
     4741 cgccgcgaag ttgcacacga tccctatcga gagcatgtcc tatcacgcgg agcgcaacga
     4801 cttctcccgg tggctcaagg cgcgcaccga attcgcgctg gcccaccgtc tccgcccgcg
     4861 caagatcggg cagttcgaca gcgccgaagc catgaaagcc gacctcatcg aggcggtgga
     4921 ggagtaccgg cgcgagcagg gtcgcacgat catcgcggat ttcgaccacc gctccttcga
     4981 caccaccagc agcttcagcc gtatcggcgg gggctcgctg ggcggcaagg cgcgggcgct
     5041 ggccttcgta cgccacctcc tcggcgcgtt cgacatgttc gcacgcttcc cgggagtaca
     5101 gatcgcggtg ccaccatcgg tggtgctcgg cactgacgtc ttcgaccagt tcctcgaccg
     5161 caacgaccta cgcgacctgg ccatccactc cgacgacgac gacgagatag agcgccggtt
     5221 ccaagccgcc gccctccccc acgaagtcac ccgtgacctg gcctcgttcc tagagctgat
     5281 ccgctatccg ctggcggtgc gctcgtcgag tctgctggaa gactcgcagt accagccgct
     5341 ggccggcgtc tacgagacgt tcatgctgcc gaacaaccac cccgacgccg gggtcagact
     5401 ccgccaactc gaagcgacca tcaagcgggt ctacgcctcg accttctcct cccacgccaa
     5461 gagctacctg gaagccaccc tctaccgctt agaagaagag aagatggcgg tgatcatcca
     5521 gaaggtggtg ggagcgactc acgaggagcg gttctatccg gacatctccg gcgtggcccg
     5581 ttcgtacaac ttctacccca cctcacccat gaccggcgag gatggggtgg cgatcgtcgc
     5641 gctggggttg gggcgcacgg tggtcaaggg agagcgcgcg cacagcttct gtccgaagta
     5701 tccgcgccac cagctcatgg cgggtagcct cgaagacacg ctgcgcaact cccagcgtga
     5761 gttctgggcg gtggagctcg gaccgttcgc caacgatccg gatgccggcg aaccggcccc
     5821 ggacctctgg gagaagaaat acccgctcga ggtcgctgag gaagacggca ctctcgccca
     5881 gctcggctcc acctattcgc cggagaacga cgcgctctac gatggcgtct cgcgcccggg
     5941 ggtccgcgta gtcaacttcg cccccttgct caagttcgac ctcttcccgc tggccgacat
     6001 cgtggatctc ctgctcgaga tcggcgccca aggcatgggg actccggtgg agatcgagtt
     6061 cgccgcgaac ctgaccgcta acgagggagc ccccaaggag ttcgccttcc tccaggtacg
     6121 gccgatgggc ttctcgagag agatggagga tctcaccctc gaggagtccg ggtcggaacg
     6181 agtgttgtgc cacagcacca gcatcctcgg aaacggccgg ttgaacggga tcaaggacgt
     6241 cgtcgtggtc gacatcagcc gcttcgaccg ctcgcgcagt ctggaggtcg cccgagaggt
     6301 ggcccgcttg aacgcacggc tcacccgcga aggcgctcgc tacatcctca tcggtgtcgg
     6361 ccgctggggt tcggccgacc cgtggctcgg gatcccggtg aattgggacg agatcgccgg
     6421 cgcgcgggtg atcgtcgaat ccgaactcaa ggacatcccc gtgacgccct cgcaagggag
     6481 ccacttcttc cagaacctga cctcgttccg cgtgggctac ttcactgtca atcccgaggc
     6541 gggcgccgga cgggtcgact gggagtggtt ggccgcccag cccgccgcaa gcgagggtga
     6601 gttcgtgcga cacctgcgct tcgaccaccc cttggtggtc agcatgaacg gcacgacgcg
     6661 cgaagggatc atcctcaagc cgaaggagta ggccggccgg ccggaccgtc ctcgcgccga
     6721 acgagcagac ggtccggcgg gccgccgagc cgcaggagta ggccgaccgc cgggcaatcg
     6781 ggcggccgac cgacagccgc tccgtgttta gccgggcgcc cctcccgggc attcccgtga
     6841 cgtgccaacg cgactaggag accacaccat gaaccagtcc cacgtcaagc tctacgcgct
     6901 cagcacgtgc ggtcattgcc ggaacgtcaa gaactttctc aacgagaacg gcatcgagta
     6961 ctcctccgtc gaggtggatc tcacgacagg cgaggagcgc cagaggatca tcgacgaggt
     7021 gcgtgccctc aaccccaaca cctcctttcc gaccatcgtc atcggggaga cggtcatcgt
     7081 ggggttcaag gaggcggcca tgagggaggc gcttggactg tgagagacgc ggtgcgccaa
     7141 ct
//
LOCUS       DASRMJ010000182         5113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_234331, whole genome shotgun sequence.
ACCESSION   DASRMJ010000182 DASRMJ010000000
VERSION     DASRMJ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234331"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFD74_05000"
     CDS             complement(<1..725)
                     /locus_tag="VFD74_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HZK48940.1"
                     /translation="MRYRYPKLNTEMPDRTDVLRLTPSAALPPLAEHMASLDLPSYLD
                     RSDAQQRLCSWGLDGTCCRRCLWGPCRVTISKPGICGAGPAQVVMGNHLRMVAAAAAA
                     HGSHARARLETILAITEGREEGPLRGMDRVRWLAERLGVPQASAGADRPDLDVARDVA
                     LVLYDDLSKARPGPMRLLHACAPPERVETWRELGVLPTSTMAEVFESLHRTTLGTDSD
                     WRNTALQELRLAVAFFWGAVAPA"
     gene            complement(735..1619)
                     /locus_tag="VFD74_05005"
     CDS             complement(735..1619)
                     /locus_tag="VFD74_05005"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK48941.1"
                     /translation="MERVNGAPERDGSDPRTSGPAPGGTGQAGVRRAGLVDEIIVVDG
                     AGRTFGRGNTAVEALAATTLEVYEGEFFCLVGPSGCGKSTLLNLVAGFDRPDRGRVLV
                     QGRPVTGPDPRRLLVFQDYGLLPWRSAIGNVELGLEIMGIPREERRSRARALLERVGL
                     SVFEDKKPHELSGGMRQRVNIARSLAVDPDILLMDEPFGAVDALTRMGLQADLLELHR
                     AGGKTVVLVTHDIEEAIYLGDRVAVMSERPGAVRATVNVDLTWPRDRTSAEFTHLREV
                     ILEHFRLVPERRAPEYSI"
     gene            complement(1607..2419)
                     /locus_tag="VFD74_05010"
     CDS             complement(1607..2419)
                     /locus_tag="VFD74_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK48942.1"
                     /translation="MSTAPVAARRIAGRQWGSWKPRLGDVGLRLLGVLAFFSVWQAAT
                     SAGLLGEAFGADFGPVRGLRALGRLTASGVLVEQSSASLGRLGLGLGIAVLIGAPLGV
                     LVGYSRPLDAATSVVFQFLRMTSPLALVPVALVAFGVGSRPVVFLVAFAAVWPVLLST
                     AHGVGRVSPIWKRVVRGFGGGDRAVVRKVILPATVPDLLQGVRLALGVGWVVIVPAEM
                     LGVDSGLGYQILDARDRFAYDELVATILAIGLLGYLTDLALRWLKERYSWSA"
     gene            complement(2416..3687)
                     /locus_tag="VFD74_05015"
     CDS             complement(2416..3687)
                     /locus_tag="VFD74_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK48943.1"
                     /translation="MSAQAITRRQLLKLAGVSGLGLSGLGWATGCASDATPSTAVTGA
                     TGVSPTQTAADAGSETQLPPVKIGYIPITDATPLLIAYANGYFQEEGLEVEQPTLIRG
                     WSALAEAFQAGTFNLTHLLIPIPIYLRYARDFPVKVVAWNHMNNSALTVRDQGSIQST
                     ADLGGKQIAVPYWYSMHNVILQMVLRSHGLRPVIQNQKAPLAADQTNLLVMAPPDMPT
                     ALSAGAIDGYIVAEPFNAAGEVLAGGRVIRFTGDVWQNHPCCVAVLHEGDVRERREWS
                     QKAVNALVRAERWSRENKEEAARILSKEGRGYLPFEEKIILRSMTKYDLETYGVSGGT
                     GAIRHPEWEASRIAFEPYQFRSATKRIVQELKQTVLDGDTTFLQDLSPEHVADDLMDY
                     ELVKKALAASGGLVAFDGAYPDFERDEVIEV"
     gene            complement(3822..4037)
                     /locus_tag="VFD74_05020"
     CDS             complement(3822..4037)
                     /locus_tag="VFD74_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK48944.1"
                     /translation="MFKAEDLPHIFKRFYRVDKSRSTAGGGTGIGLAVARSLLRQMDG
                     DISATSTPGRGTASFTLPAARPAESTS"
     gene            complement(4513..>5113)
                     /locus_tag="VFD74_05025"
     CDS             complement(4513..>5113)
                     /locus_tag="VFD74_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK48945.1"
                     /translation="RDLGLHEAGVELDDTGFVRVDEYLRTSNPRVYAAGDVAGSAGGS
                     QPATPVGAHDGGIAARNALADAQQKVDHRVIPRAIFVDPLVASVGLTDLEANRAGHRC
                     SCRTVPLTLVPRAAAVHSARGFVKMVLDRDSREVLGVTMVGQGAPEVIHEGAMGMRFG
                     AKEHDFVDMIHVYPTMAEALKIVALSFTKDVSKLSCCAE"
BASE COUNT          851 a         1735 c         1704 g          823 t
ORIGIN      
        1 ctcgcggggg ccacggcccc ccagaagaag gccacagcga gccgcagttc ctggagggct
       61 gtgttccgcc agtcggagtc ggtgccgagc gtggtccgat gcaggctctc gaacacctcg
      121 gccatcgtgg aggtggggag cacgccgagc tcccgccagg tctccacgcg ttccggcgga
      181 gcgcaggcgt gcagcaggcg catgggtccc ggtcgggcct tcgagagatc gtcgtagagg
      241 acgagggcga cgtcccgcgc cacgtcgaga tcgggccggt cggcaccagc ggaggcctgc
      301 ggcacgccca gccgttctgc gagccagcgc acccgatcca tcccacgcaa gggtccctcc
      361 tcgcgaccct ccgtgatggc gaggatggtc tcaagtcgtg cccgcgcgtg cgaaccgtgc
      421 gcggcggccg cggcggccac catgcggaga tggttgccca tcaccacctg ggccgggccc
      481 gccccgcaga tgccgggttt ggagatggtc acccggcagg gtccccacag gcaccggcgg
      541 cagcaggtgc cgtcgaggcc ccaggagcag agtcgctgct gggcgtccga gcgatccagg
      601 tagctgggga ggtcgagcga ggccatgtgc tccgccaggg gcggaagggc cgccgaaggg
      661 gtcagacgca ggacgtccgt tcggtcgggc atctcggtgt tcagcttggg gtagcgatag
      721 cgcacggcct ctcctcagat cgaatactcg ggtgcacggc gttccggcac caatcggaag
      781 tgctcgagta ttacttcacg caggtgggtg aactcggcgg aggtgcggtc ccgcggccac
      841 gtgaggtcca cgttgacggt ggcccggacg gcgcccggac gttcgctcat caccgcgacc
      901 ctatccccca gatagatggc ttcctctatg tcgtgcgtca cgaggaccac ggtcttgccc
      961 ccggctcgat gcagttcaag gagatcggcc tgcaatccca tccgggtgag cgcgtccacg
     1021 gcgccgaatg gctcgtccat gagcaggatg tcggggtcca cggccaggga gcgggcgatg
     1081 ttcacgcgct gacgcattcc gcccgagagc tcgtgtggct tcttgtcctc gaacacactc
     1141 aggcccacgc gctcgagcag ggcgcgggct cgggatcttc tctcctcgcg cgggatcccc
     1201 attatctcca ggcccagctc cacgttcccg atggcagagc gccacggcag gagcccgtag
     1261 tcctgaaaca cgagcagccg gcgcgggtcg ggccctgtga ccggtcgacc ctgcaccagc
     1321 acccgccctc ggtcgggccg gtcgaacccc gcgaccaggt tgaggagcgt gctcttccca
     1381 cagccggatg gaccgaccag gcagaagaac tcaccctcgt acacctcgag ggtggtggcg
     1441 gccagcgcct ccacggccgt gttccctcgt ccgaacgtgc ggccggcgcc gtcgacgaca
     1501 atgatctcgt ccacgaggcc ggcccgacgc acgccggcct gtcccgttcc gcccggtgca
     1561 gggcccgagg tgcgcgggtc cgagccgtcg cgctccgggg ccccgtttac gcgctccacg
     1621 agtacctctc cttgagccac cgcagagcca ggtctgtgag atacccgagc agcccgatgg
     1681 cgaggatggt ggccaccagt tcgtcgtagg cgaagcgatc ccgggcgtcg agtatctggt
     1741 accccagacc ggaatcgact ccaagcatct cggccggcac gatcaccacc caacccacgc
     1801 ccagggccag acgcacaccc tgcaggagat cgggcacggt ggccgggagt atcaccttcc
     1861 gcacgaccgc ccggtcacct ccgccgaaac cgcgcactac ccgtttccag atggggctca
     1921 cacggcccac gccgtgcgcc gtcgagagca ggacgggcca cacggcggcg aacgccacca
     1981 ggaagacgac cggccgggac cccacgccga acgccaccag ggccacgggc accaacgcca
     2041 gcggagaggt catgcgcaag aactggaaga ccacgctggt ggctgcgtcg aggggtcgcg
     2101 agtagcccac gagcaccccc aagggcgctc cgatgagcac ggcgatcccc aggcccaacc
     2161 ccaggcggcc gagggaagcc gacgactgct cgacgagcac gccggaggcc gtcaaccggc
     2221 cgagtgcgcg caggccccgc accggtccga agtccgcccc gaaggcttca cccagcagtc
     2281 ccgcgctcgt tgccgcctgc cagacggaga agaaggccag gacgccgagc agtctcagac
     2341 cgacgtcgcc gagacgaggt ttccacgatc cccactgtcg ccctgcgatc cgccgcgcgg
     2401 cgacgggagc ggtgctcata cctcgatcac ctcgtcccgt tcgaagtccg gataggcgcc
     2461 gtcgaacgcg accagacccc cgctggcggc caacgccttc ttcaccagct cgtagtccat
     2521 gagatcgtcg gccacgtgct cgggggacag atcttggagg aaggtcgtgt ccccgtcgag
     2581 tacggtctgc ttcagctcct gaacgatgcg cttcgtggcc gagcggaact ggtagggctc
     2641 gaaggcgatc cgcgacgcct cccactcggg atgccggatg gctcccgtgc ctccggagac
     2701 gccataggtc tccaggtcgt acttggtcat ggagcggagg atgatcttct cttcgaacgg
     2761 taggtagccg cgaccctcct tcgagagtat tcgcgccgcc tcctccttgt tctctcggga
     2821 ccagcgctcg gcgcggacta gagcgttgac ggctttctga ctccactccc gccgctcgcg
     2881 cacgtcgcct tcgtggagca ccgcgacgca gcacgggtgg ttctgccaga cgtctcccgt
     2941 gaaacggatc acccggcccc ccgcgagcac ctcgcccgcc gcgttgaacg gttcggccac
     3001 gatgtagcca tcgatcgcgc ccgcgctcaa ggccgtgggc atatcgggcg gagccatcac
     3061 cagcaggttg gtctgatcgg cggccagggg agccttctga ttctggatga ccgggcggag
     3121 cccatgagag cgcagcacca tctgcaggat gacgttgtgc atggagtacc agtacggtac
     3181 ggcgatctgt ttgccgccca agtcggccgt gctctggatg gatccttgat cgcgcacggt
     3241 cagggcggag ttgttcatgt ggttccaggc gaccaccttc acggggaagt cgcgcgcgta
     3301 ccggaggtag atggggatcg ggatcagcag gtgggtgagg ttgaacgtgc ccgcctggaa
     3361 ggcctccgcc agtgcggacc acccccggat gagggtgggc tgctccacct cgaggccctc
     3421 ttcttggaag tacccgttcg cgtacgcgat caggagtggg gtggcgtcgg tgatagggat
     3481 gtagccgatc tttaccgggg ggagttgggt ctcgcttccc gcgtccgccg ccgtctgcgt
     3541 gggcgacacg ccggtcgctc cggtcaccgc ggtgctaggc gtggcgtcgg aagcacatcc
     3601 tgtggcccag cccaggccgc tgaggcccag gccggagaca ccggccagct tgagtagttg
     3661 ccgtcgtgtg atcgcttgcg cgctcattcc ctcggccctt cgaatccgta cgacaatata
     3721 aagttgagta agtgtatcag aatactcaac tttatgcgga gcatgccatc ggccgggtgg
     3781 aagtccaatg ctccttcttc gcgagaagat cctgagggct gctagcttgt actctctgcc
     3841 ggccgcgccg cgggcagtgt gaacgacgct gttcctcggc cgggagtgct ggtggccgag
     3901 atgtctccgt ccatctgtcg caacagagac cgggcgaccg cgagtccgat ccccgttccc
     3961 cctccggctg tggagcgcga cttgtccacg cggtagaagc gtttgaagat gtgaggcagg
     4021 tcttccgcct tgaacactat ggtagccccc gcgctccggt tgcgccgcta cgagccgacg
     4081 gacgttctta ccaactctgc aagggctgcc tcaagcgctc gtgggtagct cccgattcgg
     4141 acccaacggg agctccgtca cctcggcagg gtccggatcg gcaggaacgt aggcggcgag
     4201 gcagcgaggg cagcagaagt acacggttct gaagccattg tgcgccgaat gaggtgcagt
     4261 gtgcggatcg acgaacattc cgcagatggg atcgggaacc aggccccggg gggccgacac
     4321 catgacatca tggtgtgggg gtcgtgcttc gcaccgtcgg tgattgttcg aatccaacaa
     4381 cagcccttga ggagcacccg ggtctggcca gcagttggag taaacgcctc cgccgaggac
     4441 catgaaccgc atggagagcc gagtgactcg catcgctcgg cattcactct ctcgtcgcaa
     4501 cgcggtgcct tctcactccg cgcagcaact cagcttgctc acatccttcg tgaagctgag
     4561 ggcaacgatc ttcagggcct ccgccatggt gggatagaca tggatcatat ccacgaagtc
     4621 gtgctctttg gctccgaaac gcatacccat ggccccctcg tgaatcacct cgggggctcc
     4681 ctgacccacc atggtcaccc cgaggacctc ccgggagtcg cggtcgagca ccatcttgac
     4741 gaagccgcgg gcggaatgga cggccgcggc ccggggaacc agggtgaggg gcactgtgcg
     4801 gcaggagcag cggtgacccg cccggttggc ttcaagatcg gtgagaccca cgctggccac
     4861 cagcgggtcg acgaagatgg cccgggggat cacccggtga tccactttct gctgcgcgtc
     4921 ggcgagcgcg ttccgggcgg cgatgccgcc gtcgtgggct ccgaccgggg tggccggttg
     4981 actccccccc gcgctccccg ccacgtcgcc cgcggcatag acgcgggggt tgctggtccg
     5041 caggtactcg tccacccgta cgaagcccgt gtcgtccaac tccacgccgg cttcgtgcag
     5101 ccccaggtcg cgg
//
LOCUS       DASRMJ010000183         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_50164, whole genome shotgun sequence.
ACCESSION   DASRMJ010000183 DASRMJ010000000
VERSION     DASRMJ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50164"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VFD74_05030"
     CDS             <1..738
                     /locus_tag="VFD74_05030"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48946.1"
                     /translation="EILELPPGVIQDRWHLNDGEGAAHLFMGKVTKGMMGGGFLYTNK
                     ESISLGIVVGMQTMKDRREKVDSYTLLDEFKEMPQIKPLVEGGTLVEYSAHAVPEGGI
                     AKVPKLFGDGYLMVGDAAGLALNALITVRGMDFAIASGFYAARAAIDAKARGDFSAAG
                     LAQYERSLRSSFVLKDLQTARDIPHTIENPRLFTHYPQAVSSLLEKLFTIGPQPSRKL
                     SSTVISAVRKDFLNPTTLRELWAMRKI"
     gene            750..1025
                     /locus_tag="VFD74_05035"
     CDS             750..1025
                     /locus_tag="VFD74_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZK48947.1"
                     /translation="MNIEGKLGLDVFKIDKERHITIDVEVCNAVCTNKACLYVCPAHL
                     YEIDDQGNNTVNYEGCLECGTCLICCDHRALTWHYPRGEYGVQYRMG"
     gene            1130..1918
                     /locus_tag="VFD74_05040"
     CDS             1130..1918
                     /locus_tag="VFD74_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZK48948.1"
                     /translation="MRLDIVVCVKTNPDLQMIRIKNREAVLEAIPFKAGDLEKNAVEA
                     ALQLRDAAGDGKVVVLVVAEGNRKVRETVKEVLAMGADEAVLVVDPALETADQAGVAA
                     ALAAALQKIGSYDLVLFGEGSTDNYTGQVGPRVAEILGLPQIGYARKITAGGTGVLAE
                     RSMDDSIETLEAPLPAVVTVVSEINEPRIPSLMNIMKAGKKPVEEWSLADMGLDPAAV
                     APVRTILSNLAEEQDRKHIMFAGGAAEQAEAFVNALIKEGVLGR"
     gene            1921..>2751
                     /locus_tag="VFD74_05045"
     CDS             1921..>2751
                     /locus_tag="VFD74_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZK48949.1"
                     /translation="MADILVYSEKQEAVRELTFKAKEFAGALGMGVSAAVLGSGSDAA
                     GDLGEFGADRVYVSDDPALEGLAVDVTAEALAQVAREAGATVVLVASTRRGKELAPRL
                     AQKLGAGCVSDVSSLGLEGSTLVATRYAFGGNTVAAETLDTEVKVFAVMPKTFEIGAP
                     TPGAGQIVRPALTLQPSAVKLVERRAKEGDAVNLEDAERIVGIGRGLSKREDVELGRE
                     LAAALGAELSCTKGLADFGWLGEDRIIGLSGAKTKPELYLSVGISGQIQHTVGISSSK
                     L"
BASE COUNT          523 a          842 c          923 g          463 t
ORIGIN      
        1 gagattctgg agcttccccc cggtgtgatc caagaccgct ggcacctgaa cgacggtgaa
       61 ggcgccgctc acctcttcat gggcaaggtg accaagggca tgatgggcgg cggttttctc
      121 tacacgaaca aagaatcaat ctccctgggg atcgtggtgg gcatgcagac gatgaaggac
      181 cgtcgggaga aggtggactc gtatacgttg ctcgatgagt tcaaggagat gccccagatc
      241 aagcccctgg tagagggcgg gacgctggtt gagtattccg cgcacgccgt ccccgagggg
      301 ggcatcgcga aggtgcccaa actcttcggc gacggctacc tgatggtggg agacgccgcg
      361 ggtcttgccc tgaacgcact cattacggtg cgcggcatgg acttcgctat agcttccggg
      421 ttctacgccg cccgagccgc gatcgatgcc aaggcacgcg gagacttctc ggcggccggg
      481 ttggcccagt acgagcgatc gctgcggagc agtttcgtac tgaaagatct gcagacggcg
      541 cgtgatatcc cgcatacgat cgagaacccc cgtctcttca cgcattaccc gcaggccgtc
      601 tcgagcctgc tcgagaagtt gttcaccatc ggtccccagc cgtcccggaa actctccagc
      661 accgtcataa gcgcggtccg caaggacttc ctgaacccca ccacgctcag ggaactctgg
      721 gccatgcgga agatctaggg aggggcgaga tgaacatcga aggcaaactg gggctcgacg
      781 tcttcaagat cgacaaagag cgtcacatca cgatcgacgt ggaagtgtgc aacgcagtct
      841 gcaccaacaa agcctgtctc tatgtgtgcc cggcgcacct ctacgagata gacgaccagg
      901 gcaacaacac cgtcaactac gagggctgct tggagtgcgg cacctgcctc atttgctgtg
      961 accaccgtgc actcacctgg cactacccga ggggcgagta cggagtgcag taccgcatgg
     1021 ggtaggtccg cacctgccct gaccgtcgcg cggctcaacg tgactgtcgc accggttgct
     1081 tccaacgctc gggctctgtc gcgagcgcga tccacacgag agaagggtga tgaggttgga
     1141 cattgtcgta tgcgtcaaga ccaatccgga tctgcagatg atccgaatca agaaccgaga
     1201 ggcggtcttg gaggccatcc ccttcaaggc gggcgatctg gagaagaacg cggtcgaggc
     1261 cgccctgcaa ctgcgtgacg ccgccggcga cggcaaggtc gtcgtgctcg tggtggccga
     1321 gggtaaccgc aaggtgcgcg agaccgtgaa ggaagtctta gccatgggag ccgacgaggc
     1381 cgtcctggtc gtcgacccgg ctctggagac cgccgatcag gcaggcgtgg ccgccgccct
     1441 agccgccgcg ctgcagaaga ttggatcgta cgacctcgtg ctcttcggtg aaggctccac
     1501 cgacaactac acgggtcaag tgggcccgcg ggtggccgag atcctcgggc tcccccagat
     1561 cgggtatgcc cgcaagatca ctgccggggg caccggcgtg ctggccgagc gcagtatgga
     1621 tgactcaatc gagaccctgg aagctccttt gcccgcggta gtgactgtgg tcagcgagat
     1681 caatgaaccc cgcatcccct cgctcatgaa catcatgaag gccggcaaga agcctgtgga
     1741 ggagtggagc ctggccgaca tgggcctgga cccggcggcc gtggcgccgg tgcgcaccat
     1801 cctctccaac ctggcggagg agcaggaccg taagcacatc atgttcgcgg gcggcgcagc
     1861 cgagcaggcc gaggcgttcg tcaacgcgct catcaaagaa ggggttctgg ggagatgacc
     1921 atggccgata tcctcgtata ctcagagaag caggaagcgg tccgtgagct caccttcaaa
     1981 gccaaggagt tcgccggtgc tttgggcatg ggtgtctccg ccgcggttct cggatccggc
     2041 tccgacgccg caggcgacct gggcgagttc ggcgccgatc gggtctacgt cagcgacgac
     2101 ccggcgctgg aaggtctggc cgtggacgtg acggctgagg ccttggcgca ggtggcccgc
     2161 gaggcgggtg ccacggtggt cctggtggcc tccacccggc gcggtaagga gttggcgccg
     2221 cgcctcgcgc agaagctcgg cgcgggctgc gtctccgacg tgagttcgct gggcctggag
     2281 ggctccaccc tggtggccac tcggtatgcc ttcggcggca acaccgtggc agcggagacc
     2341 ctcgacactg aggtcaaggt gttcgcggtc atgcccaaga cgttcgagat cggggcgccc
     2401 acgcccggcg ccggccagat cgtaaggccg gcactcacac tgcagccctc ggcggtcaag
     2461 ctcgtggagc gtcgcgcgaa agaaggcgac gctgtcaacc tcgaagacgc cgagcgcatc
     2521 gtggggatag gtcgcggcct gagcaagcgt gaggacgtgg aactcggccg ggaactggcc
     2581 gccgcgttag gcgccgagtt gtcctgtacg aagggactgg cggatttcgg ctggctcggt
     2641 gaggaccgca tcatcggtct gtccggcgcc aagacgaagc ctgagctgta cctctcggtg
     2701 ggcatctccg gccagatcca gcacacggtg ggcatcagct cgtctaagct c
//
LOCUS       DASRMJ010000184         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_303257, whole genome shotgun sequence.
ACCESSION   DASRMJ010000184 DASRMJ010000000
VERSION     DASRMJ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303257"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VFD74_05050"
     CDS             <1..1105
                     /locus_tag="VFD74_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK48950.1"
                     /translation="TPPTKKPLGYLGSPRGFFVGGVSERFPLGAPGPTAFARDVLGRE
                     FLAADAEETPLTEDDLVSLLERTRCVVAGGDSAGRIGAAAPVEQDASAVAAPAARLRG
                     VSLAYNRGTFLERKALTGVDVAITSGVVTAVVGATASGKSTLLQLVAGLLRPDSGSAE
                     YLGAARPAPGRVGMVFQRPETQLFKSTVWDDVAVAPRLHGLVGAELERRVESALWIVG
                     LDPEEFGGRAPHGLSLGEQRRVALAGVISLAPSLLVLDEPGAGLDPLARDRLMSRLAD
                     WVSGAASETRAGGGTGATAGIAPRSLVFSSHDIDEVARRADRVVVLDQGAVLAEGPVT
                     DILSDIRLLAVARLRPPLAARVAARLGAPPKCG"
     gene            1191..2006
                     /locus_tag="VFD74_05055"
     CDS             1191..2006
                     /locus_tag="VFD74_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZK48951.1"
                     /translation="MSRAGLTIPIGQYFPAVTAVHGLDPRTKLLAALGFAVTLFIVTG
                     WLGIGVLGAGLAVVLAAARVPPTYLWRSLRPLLFLLALTFLFQMFSFPGEPVVSLGPL
                     SVTREGLSRGGFLTVRLGLLLISSAALTLTTPPVLLTDGIEWLLRPLRRVRVPSHEIA
                     LMMTIALRFIPTLLRELEDLLKAQRARGVDLSGRDPRRALSALLPLVIPLFVISFRRA
                     DDLAVAMTSRCYRGDVGRTRYRELKAGWRDAAAAVVSIALLGLAVTAGRVWGV"
     gene            1994..2881
                     /gene="truA"
                     /locus_tag="VFD74_05060"
     CDS             1994..2881
                     /gene="truA"
                     /locus_tag="VFD74_05060"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZK48952.1"
                     /translation="MGGLSMGDVSMGKLSLGGSHTYRLDLQYDGTHFRGWARQPGQVT
                     VEESLEKALAVALRHTVRLSVAGRTDSGVHAMRQVAGFVLPTADSLPEAQGGVPEATW
                     AALADEEGRGRLLLSVNALTPAGLVVRALSEAAPSFDARRDATARSYRYFIWRGLTPS
                     PFMERYSWHVRHDLDVPAMEEAARLCLGRHDFTAFTPTESHHTMFHRDIQRCVWRSRG
                     PLLWLEVRAPHFLRHMVRALVGTMVEIGRGRMPLEHLEMLLDGAERLEAGRTAPPNGL
                     FLWRVFYGRGKGKEEWASP"
     gene            2933..3367
                     /gene="rplM"
                     /locus_tag="VFD74_05065"
     CDS             2933..3367
                     /gene="rplM"
                     /locus_tag="VFD74_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZK48953.1"
                     /translation="MKTYSAKPGEVDRKWWVVDAEGKNLGRLATRLAIVLRGKDKPQY
                     TPHTDTGDFVIVVNAGKVSVTGNKLESKRYYRHSGRPGGLKSRTLGEMLHQKPTEVVR
                     HAVKGMMPKNRLSAQQLGKLKIYATAAHPHKAQEPEELKWEQ"
     gene            3355..3747
                     /gene="rpsI"
                     /locus_tag="VFD74_05070"
     CDS             3355..3747
                     /gene="rpsI"
                     /locus_tag="VFD74_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZK48954.1"
                     /translation="MGAVVYSATGKRKTSVARVRITPGTGVITANGKTLNEYFNRATL
                     VTAARSPLVESGTEGRYDVVAQLHGGGKSGQAGALKHGIARALVEADPGLRPEIKKAG
                     HLTRDARMVERKKAGLKKARKRPQFSKR"
     gene            3827..>4710
                     /locus_tag="VFD74_05075"
     CDS             3827..>4710
                     /locus_tag="VFD74_05075"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZK48955.1"
                     /translation="MAELRVDLDGIRHNTAAVAALLARVDMRLVAVTKGCVGDVRVAR
                     AMLDGGAQALADTRDAHLRRLREAFPGVELHRIYLPSFSSAFEPGDISYVSSVEAAIR
                     VAEQGCASGRGSVMVQVETGDLREGVPPDGLIPLLEAVAADPRLEPAGVSTNYACFDG
                     DAAGIRESVRSLAAAVQAARAAGFEVRRVSGGNSSLLALLVDGVELPSEITELRCGEA
                     LLLGQDALVYRPLPGCRRDAVVLRAEVLESYTKASREGTCRRLVLGLGHQDIGCGKVE
                     FRFPELREVGRSSDYMVVV"
BASE COUNT          690 a         1512 c         1685 g          823 t
ORIGIN      
        1 cactcctccc acgaagaagc ctctgggcta cctcgggtcg cccagaggct tcttcgtggg
       61 aggcgtgtcc gaaagattcc ccttgggcgc tccaggaccg accgcgttcg cgcgcgatgt
      121 gctgggccgc gagtttctcg cggccgatgc agaggagact ccactcaccg aagacgacct
      181 ggtctcgctg ttggagcgta ctcggtgcgt cgtcgcaggc ggcgactcgg ccggaaggat
      241 cggtgcagcc gcgccggtcg agcaggacgc gtccgctgtg gcggctcccg cggcgcggct
      301 gcggggagtg tcgctcgcgt ataaccgcgg cacgttcctc gagcggaagg ccttgacggg
      361 cgtcgacgtc gcgatcacgt ccggagtggt cacggcggtc gtgggtgcca cggcatcggg
      421 gaagagcacg ctcctccaac ttgtcgcggg cctgctgcgt cccgattccg ggtcggcgga
      481 gtatcttggc gcggcccgcc ccgctcccgg tcgtgtgggc atggtgttcc agcggcccga
      541 gacccagctc ttcaagagta cggtgtggga cgacgtggcg gtggctccgc gattgcatgg
      601 gctggtcgga gcggagctgg agcggagagt ggagtcggcc ctgtggatcg tggggctcga
      661 tcccgaggag ttcggcgggc gggcgccgca tggcctgtcg ctgggggagc agcggcgcgt
      721 ggcgctcgcc ggggtgatct cgttggcccc gagcctcctg gtcttggacg agcccggcgc
      781 cgggctcgac ccgctcgctc gggacaggtt gatgtctcgg ttggcggatt gggtgtccgg
      841 tgcggcgagt gagacccgtg cgggcggcgg aaccggcgcg accgccggga ttgcgccgcg
      901 ctcgctcgtg ttcagttcgc acgacatcga cgaggtcgcc cgccgagccg accgcgtagt
      961 cgtgctcgac cagggcgctg tgctggctga aggtccggtg accgacatct tgagcgatat
     1021 ccggctgctg gcggtggccc gacttcggcc tcctctggcg gcgcgcgtgg cggcgcgcct
     1081 aggcgcaccg ccgaagtgcg ggtgatcgac gccgcggggt tcacctcatg gttcaggtcc
     1141 gcgtcgccgg tgggaccggt ctccgtcagg accgcaggag agggcgggac gtgagcagag
     1201 cggggctgac catccccatc ggccagtact tcccggccgt gaccgctgtg cacggcctcg
     1261 atccgcggac gaaactgctc gccgccttgg gcttcgccgt gaccctgttc attgtgacag
     1321 gttggctggg gatcggggtc ctcggcgccg gactcgccgt ggtgctggcg gccgccaggg
     1381 tacctcccac ttacctatgg cggagcctgc gtcccctcct cttcctgctg gcgctgacct
     1441 tcctgttcca gatgttcagc ttccccgggg agcccgtcgt gagtctggga ccgctgtccg
     1501 tcacccggga gggtctctcc cgcggcggtt tcctcaccgt ccgcctcgga ctgctcttga
     1561 tctcgagcgc cgccttgaca ctgaccacac cgccggtgct cctcacggac ggcatcgaat
     1621 ggctgctgcg accgctgcgc cgcgtgcggg tgccgagtca cgagatcgcg ctgatgatga
     1681 ccatcgccct gcgcttcatc cctacgctgc tccgcgagtt ggaggacctc ctcaaagccc
     1741 agcgggctcg gggggtcgat ctctcgggtc gcgatccgag acgcgcgctg tccgcactcc
     1801 tgccgctggt gatcccgctg ttcgtcatca gcttccgtcg ggcggacgat ctggcggtgg
     1861 ccatgaccag ccggtgctac cgaggcgacg tgggtcgcac gaggtatagg gaactgaagg
     1921 ccggttggcg tgacgcggcc gcggctgtcg tgtcgatcgc cctgctcggc cttgcggtga
     1981 cggcgggccg ggtatggggg gtctgagcat gggggacgtg agcatgggga agctgagcct
     2041 agggggtagt catacctatc gtctcgacct tcaatatgac ggcacgcact tccgaggttg
     2101 ggcccggcaa ccggggcagg tgacggtgga ggagagcctg gagaaggcgc tggccgtcgc
     2161 tctcagacac acggtccgac tctccgtcgc cggtcgcacc gactcggggg tgcacgccat
     2221 gcggcaggtg gccggcttcg tgcttccgac ggcagactcg ctgccggaag ctcagggcgg
     2281 cgtgcccgaa gccacctggg ccgcactcgc ggacgaagag ggccggggtc ggctccttct
     2341 ctccgtgaac gcgctcacac ccgccggcct ggtcgtgcgc gcgctgtcgg aagccgcccc
     2401 gagcttcgac gcgcgtcgtg acgcgaccgc gcgcagctac cgctacttca tctggcgagg
     2461 cctcacgcca agtcccttca tggaacggta ctcgtggcat gtgcggcacg atctcgacgt
     2521 gcccgcgatg gaagaggcgg cccgtctctg tctggggcgg cacgacttca cggcgttcac
     2581 tcccaccgag tcccaccaca cgatgttcca ccgcgacatc cagcgctgcg tgtggcgctc
     2641 ccggggacct cttctgtggc ttgaagtccg cgcccctcac ttccttcggc acatggtgag
     2701 ggccttggtg ggcacgatgg tcgagatagg acgcggccgc atgcccctgg agcacctgga
     2761 gatgctgctc gacggggcgg agcgattgga ggcgggtcga accgctcccc ctaacgggct
     2821 gttcctgtgg cgcgtgttct acggtcgagg gaaagggaag gaagagtggg cgtcaccttg
     2881 acaccctcat caggctcctc tattatcacc gcttgcgttt gaaggaggaa gtgtgaagac
     2941 atattcggca aaacccggtg aggtcgatcg caagtggtgg gtcgttgatg ccgagggtaa
     3001 gaacctcggg cggctcgcta ctcggctcgc catcgtcctg agaggcaaag acaaaccgca
     3061 gtacacgccc cacaccgaca ccggtgactt cgtcatcgtc gtcaatgccg gcaaggtgag
     3121 cgtgacgggc aacaagctgg agagcaagcg gtactaccgt cactccgggc gaccgggtgg
     3181 cctcaagtcc cgcacgcttg gcgagatgct gcaccagaag cccacggaag tcgtgcgtca
     3241 tgctgtcaag ggcatgatgc cgaagaacag attgagcgca cagcagctgg gtaagctcaa
     3301 gatctacgcg actgccgctc acccgcacaa ggcgcaggag cctgaggagt tgaaatggga
     3361 gcagtagtct acagcgctac cggcaagcgc aagacctcgg tggcacgggt gcgcatcact
     3421 cccggcaccg gtgtcatcac cgccaacggc aagactctca acgagtactt caaccgtgcg
     3481 accttggtca ccgcggcccg ctcccccttg gtagagagtg gcacggaggg tcgttacgac
     3541 gtggtggctc aactccacgg tggcggcaag tccggccagg ccggcgccct caagcacggc
     3601 atcgcccgtg ccttggtgga agccgatccg ggtctgcgtc ctgagatcaa gaaggccggc
     3661 cacctcacgc gcgacgcccg catggtcgag cgcaagaagg ccggtctcaa gaaggcccgc
     3721 aagcgcccgc agttctccaa gcgctagtcc gagcgaggct accgatgttc ccggggcggg
     3781 ggaggagact cccccgcccc gtgttgtttc tgcggaggtt gttctgatgg cggagcttcg
     3841 cgtggatctc gacgggatcc gacacaacac cgcggcggtg gccgccttac tggcgcgcgt
     3901 cgacatgcgc ctcgtggccg tgaccaaggg gtgcgttgga gatgtccgtg tggcccgggc
     3961 catgctcgac ggtggtgcac aggccctggc cgacacacgc gatgcgcatc tgcgacgtct
     4021 gcgcgaggcg tttcccgggg tggaactcca ccgtatctac ctgccctcct tctcgagtgc
     4081 gttcgagccg ggcgatatca gctacgtgtc gtcggtcgag gccgccatcc gcgtcgccga
     4141 acagggttgt gcgtcgggcc gggggtccgt gatggtgcag gtggagacgg gagacctgcg
     4201 tgagggagtc ccgcccgacg gtctgatccc actgctcgag gccgtggcgg ccgaccccag
     4261 gttggagccg gccggcgtct cgacgaacta tgcgtgtttc gatggcgacg cggcgggcat
     4321 ccgcgagtcg gtgagatcgc tcgccgccgc ggtgcaggcc gcccgggccg cgggcttcga
     4381 ggtgcgacgg gtgtcggggg gcaactccag cctgctggct ctgctcgtcg acggcgtgga
     4441 gctgccctca gagatcaccg aactgcggtg cggagaagcg ctcctcctgg ggcaggacgc
     4501 actcgtctat cgtcccctgc cggggtgccg gcgcgacgcc gtggtgctcc gcgcggaggt
     4561 gctcgagagc tatactaagg cgtcccgaga gggaacgtgc cgccggctcg tcctggggct
     4621 cgggcatcag gatatcggct gcgggaaggt cgagttccga tttccggagt tgcgggaggt
     4681 cgggcgctcg tcggactaca tggtggtagt
//
LOCUS       DASRMJ010000185         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_339974, whole genome shotgun sequence.
ACCESSION   DASRMJ010000185 DASRMJ010000000
VERSION     DASRMJ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339974"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFD74_05080"
     CDS             complement(<1..239)
                     /locus_tag="VFD74_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48956.1"
                     /translation="MWAQKPSTLGGGYRVDGRHRRIVREEQGRRALSELEGHVVGEHA
                     QEHMGADPGFRVMVDGTDQKLGGLHLAKGVLAHFE"
     gene            complement(1138..1302)
                     /locus_tag="VFD74_05085"
     CDS             complement(1138..1302)
                     /locus_tag="VFD74_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48957.1"
                     /translation="MESLHPMSARQIVQLVRSREVSPVEVVTATLERIEGANPVLNAS
                     VAVDAEGALS"
     gene            complement(1372..2121)
                     /locus_tag="VFD74_05090"
     CDS             complement(1372..2121)
                     /locus_tag="VFD74_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZK48958.1"
                     /translation="MAETDPVRQERFILRSEGLDLRVFAAVPANEEVASPCVQIHHGG
                     GGFDPLYEQMAQQLARHGFLGVALIHRGYPGSEGCQQYGKGEVRDIGNLSEELRRRFD
                     VDPERMAIIGYSRGAHSALLALERYRDFCAGVLWSPPVDMARHVHVHPWIQDIIGGSP
                     DQVPEEYACRSPLGAVEQISCPVLILHGEQDDVVPVEHSRLLAAALEAEGKTFELEVM
                     PGEGHVWSQGGLLTVWRWTGAFLERYLMAET"
     gene            2220..>2812
                     /locus_tag="VFD74_05095"
     CDS             2220..>2812
                     /locus_tag="VFD74_05095"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZK48959.1"
                     /translation="MTVQEIKVDCGDDTIVGALHLPTGSGLPCVIISHGLYGSKDSPK
                     LTAVAEAFAAQGAAALRYDHRGCGGSGGQIAHTTVASRLEDLRTMLEVATGHPLLGGS
                     IGLLGSSMGGFISLFQALRDPRVRALSLWSTPAFIRHPERDAPPGTSLERRAFLADAP
                     HYDVRRVTRSAARCLILHGGADDVVPPFHARILHQSAT"
BASE COUNT          530 a          950 c          850 g          482 t
ORIGIN      
        1 aactcaaagt gggcgagcac gcctttcgcc agatgaagtc ctccgagctt ctgatccgtc
       61 ccatctacca tcacccggaa accagggtcc gcgcccatgt gttcctgtgc atgttcgcct
      121 actacgtggc cttcgagctc agagagcgcc cggcgcccct gctcttcacg gacgatacgc
      181 cgatggcgcc cgtcgacccg gtagcccccg ccgagcgtgg agggcttctg cgcccacacc
      241 ctctccgacc tcctggccga actcggcacc ctctaccgca atcaggtgcg catcgagccg
      301 ggggcacaac ttcaatcacg cgaccaaggc gaccgcactc cagacgcggg ccttcgagct
      361 gctcgggatc cgcccgtagc caaagctgtg ccggttccac gtgccgcaaa cccagtgatg
      421 ggacgaagat cggacgtggg cgaaaacttc cggctagatg ctgaaaccga gagcgctgct
      481 gtccgtgaac tgatggccgt taactgaatt tcttggggac gagaggagtt tacagaggat
      541 tttggacttg accagagcga atcgcccaga gcatccaggc aggagtctct cggagcagga
      601 agggatctgg ggtcgctcgg gtgataaccg gagacacaat ccagatggaa ggcggcggcc
      661 tctaccgagc ggcaaatggg ccgggggcag aaatggggtt catggtcaac aggtcacggg
      721 gacccggaaa cccgccgcag gggatatacc ccaggggcgg tctatagccg aagcatccgc
      781 aatgggcgac tggaagccgg accgaacccg cccgcatcgg ttcccgtggc cagaggcacc
      841 aggccgggat gcgctccaag ccacagggat tgcgagtgac gccgaacatg cggttcttgg
      901 tgacgagggt atgaccgaat tccggcgtgt tggtcttgcc cactatgatg gcaccgaccc
      961 gcttgaggcg gaccacctgg atcgagtcag cgacgaccac gttgtctccc gatacagggg
     1021 gaaccgaaga tggtcgccag accgaccacg tcttcgaggt ccttgactcc ccaccggaac
     1081 acgggccaga gagccgaccg gtctaccggc ggggatcgcc tcctccaact tccgagccta
     1141 cgaaagagcc ccttcggcat cgaccgcgac agaagcattc aggactgggt ttgccccttc
     1201 aatccgctcc agggtcgcgg tgaccacctc caccggactc acctcgcggc tgcgaacgag
     1261 ttggacgatc tgccgcgccg acatcgggtg gagcgattcc atggtacata ctccttgcgt
     1321 gtgcaacgct tccggatttc ccgcgggcga gtcgtgctct ggatccctct ctcaggtttc
     1381 tgccatcaga taacgctcca gaaaggcgcc ggtccacctc cacacggtga gcagcccgcc
     1441 ctgggaccag acgtgtccct ccccgggcat gacctccagt tcgaaggtct tgccctcggc
     1501 ttccagggct gcggccagca gccgtgagtg ttccaccggc acgacgtcat cctgctctcc
     1561 gtgtaggatc aggacggggc aagaaatctg ctccacggct ccgaggggag agcggcacgc
     1621 gtactcttcc ggcacctgat cggggctgcc accgatgata tcctggatcc agggatggac
     1681 atgcacatgg cgcgccatgt cgactggagg gctccataac acgccggcgc agaaatcacg
     1741 gtatcgttcc aaggccagaa gagcgctgtg ggcgccgcgg gagtacccta tgatggccat
     1801 gcgttcggga tcgacatcga atctccgacg cagttcctcg gagaggtttc cgatgtcgcg
     1861 cacctcgccc tttccatact gttggcagcc ctcggaaccg gggtatcctc tgtgaatgag
     1921 cgcgacgccg aggaagccat gccgggcaag ctgctgcgcc atctgctcgt acagggggtc
     1981 gaacccgccg ccgccgtggt ggatctgcac acagggcgat gccacctcct cgttcgctgg
     2041 caccgcagca aacacccgga ggtcgagccc ctcactgcgc agaatgaagc gctcctgccg
     2101 cactggatct gtctccgcca tgccacctcc tcgtcgaatc acgacgtgtt ccgtggttct
     2161 taccccagta tatttcacgc cagccaccgc gccgcccagg cagaaggggc caagacccga
     2221 tgaccgttca ggaaatcaaa gtcgattgcg gcgatgatac catcgtcggt gcgctccacc
     2281 tgccgaccgg ctcgggtctt ccctgcgtca tcatctccca cggcttatac ggcagcaaag
     2341 acagccccaa actgaccgca gtggctgaag cgttcgctgc ccagggcgcg gccgcacttc
     2401 gctacgacca ccgaggctgt ggaggcagtg gagggcagat cgcccacacc accgtcgcat
     2461 cgcgtctcga ggacctgcgc accatgctcg aagtcgcaac cggccatccc ctgctgggtg
     2521 gcagcatcgg gctactcggg agcagcatgg gagggttcat ctccctgttc caggcgctac
     2581 gtgatcctcg cgtccgcgct ctctccctgt ggtcgacacc ggccttcatc cgccacccgg
     2641 agcgcgacgc cccacccggt acctcgctgg agcggcgggc ctttctggcg gacgcaccgc
     2701 actacgacgt caggagggtg acccggagcg cggcgcgttg cctgatccta cacggcggtg
     2761 ccgacgacgt ggtgccgccg ttccacgccc ggattctcca ccaaagcgcc ac
//
LOCUS       DASRMJ010000186        10831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_303276, whole genome shotgun sequence.
ACCESSION   DASRMJ010000186 DASRMJ010000000
VERSION     DASRMJ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10831
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303276"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..348
                     /locus_tag="VFD74_05100"
     CDS             28..348
                     /locus_tag="VFD74_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HZK48960.1"
                     /translation="MPGPASSPPRGFVGIISAELRFGEAHSLKDKRAHIRRVRDRLTG
                     QHGASFAEIGYQDLWQRSAVVVAFAASDLAVLERRMAAALRFLDSQEWELVSVYEEVV
                     EIDE"
     gene            341..808
                     /gene="rbfA"
                     /locus_tag="VFD74_05105"
     CDS             341..808
                     /gene="rbfA"
                     /locus_tag="VFD74_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZK48961.1"
                     /translation="MSEKRYRMQRVNEALREVLSIAIGRGLKDPRVGFVTVTGVEATN
                     DLRQARVYVSILGDAAERELSLVGLRSGEGFLQGVINDELQLKRTPALEFVYDESVDR
                     GFRISKLLIAETALLGEPIDEPQGVDAEPGGETPERPHPVGPNEDLPPQTEES"
     gene            805..1785
                     /locus_tag="VFD74_05110"
     CDS             805..1785
                     /locus_tag="VFD74_05110"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZK48962.1"
                     /translation="MKSITPREVARRLSSEKEILIVSHESPDGDALGCVTALGLMARN
                     LRIPFRMYIPGEGSFPPEYSFLPLLSDIERGGFPEVTRETTAYLLDCATAERIDPTGL
                     ACAGDCINIDHHQDNRGYGTLNLLDFGATSTTQILFDVFVAGGLPVDSEIATSLYVGL
                     VTDSGRFQYSNTTPAAHRMAADLLLAGVDANAVSRGLFETMPLAKILLQARALGRLEM
                     RLGGRLVISRVDADDFADSGASEGLTEGIIDGLRRIEGVAVAAFLRERTVAGVVSQKV
                     SLRSTDGRIDVAEVAHLRGGGGHVRAAGFTVEGEARGVMDWIEREVGARL"
     gene            1782..2687
                     /gene="truB"
                     /locus_tag="VFD74_05115"
     CDS             1782..2687
                     /gene="truB"
                     /locus_tag="VFD74_05115"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZK48963.1"
                     /translation="MSVGTPRARAEGVSRVVLVDKPAGWTSYDVVRRAKGAFKGKIGH
                     AGTLDPFATGLLVVLFGQATRLSSVFMDLPKEYAVTVRFGVVSSTGDPTGELVATGGR
                     TDLPAIVSALDAFRGEIVQRVPMTSAVKVEGERLYQKAHRGETVETPERSVRVYDLAV
                     SGFDEVAQDLELLALTGKGTYVRCLAEDLGEALGVGAHAARLRRRRVGAFDVADAVAP
                     GDLQPDMLSQPDGRSVMTLSAALSFLPAFEVGEEDARLVGNGGRLCDAPSGLFRVSSG
                     GRLLALYEGSGGSAQSRVVFPTPED"
     gene            2750..3700
                     /locus_tag="VFD74_05120"
     CDS             2750..3700
                     /locus_tag="VFD74_05120"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZK48964.1"
                     /translation="MRVYRDIDEVADLGGRPRAVAIGTFDGLHRGHQEIVKRAVRAAA
                     VMDGAATVLTFEPHPSSVLAPGSDPALLSTFEYKVDLLDRAGVDEVVALPFDMAFAAL
                     SPEQFCRRILSERLNARQVMVGENFRFGHQASGSAEDLIAHGRRSGFSVTAIGLIIVR
                     GKPVSSTRIRRLVAAGRVEEAAELLGRPHVIEGFVGSGAGRGRSLGTPTANLEAVAGV
                     AVPAAGVYVTRTVVADGDVRPSVTSIGTNPTFESDGRVRIETFVLDFTGNLYGRRIRV
                     EFLQWIRGQETFTDPAALQVKIAEDVNAARSLFGEVSGRA"
     gene            3842..4108
                     /gene="rpsO"
                     /locus_tag="VFD74_05125"
     CDS             3842..4108
                     /gene="rpsO"
                     /locus_tag="VFD74_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZK48965.1"
                     /translation="MLSKERKTDIIDRFKKHEGDTGSPEVQIAILTEQINVLTEHLKI
                     HKKDHHSRRGLLKMVGRRRRLLNYLQGQEMERYRTVVKELGLRK"
     gene            4244..6379
                     /gene="pnp"
                     /locus_tag="VFD74_05130"
     CDS             4244..6379
                     /gene="pnp"
                     /locus_tag="VFD74_05130"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZK48966.1"
                     /translation="MTEQAAANQASTTTIQVGDKSILLETGRLSKQADGAVLVRSGDT
                     MVLVTAVVSKNERNVDFFPLTVDIEEGMYAAGKIPGGFIKKEGRASEQAILTARLTDR
                     PIRPLWPKGFNKETHIVATVLSVDKTNPYDVLAILGASAALSISGAPFQGPIGVVRVG
                     QVDGAWVINPTYADLESSPINLIVAGTADAISMVEAGCAEVSEADVLAALDVAHAAIK
                     IQVAGIADWAGQVGKPAMEFVERLPDRTLVERIKAGHASEILSAVSVLEKHERSAAVA
                     AVKQQVLAGLQPADGSVDAATHAAAVSKAFDAVEKEIVRKRIAIDKVRPDGRAKDEIR
                     QISSEVGVAPRTHGSGLFTRGQTQVMTLLTLGASRDEQRIDGISIDESKQFMHHYKFP
                     PFSVGEAGFMRGPGRREIGHGALAERALVRLVPEHEDFPYTIRLVSETLESNGSSSMA
                     SVCASTLAMMDAGVPIKRPVAGIAMGLVKEMDEYIILTDIAGVEDHLGDMDFKVAGTR
                     DGITALQMDIKIKGVTSAIMRDALEQALKARLFILDKMAEVIATPRTELKEFAPRIIT
                     VKVDNDKIGAVIGKGGETIRGLEAEFDVTIEIDDEGLAKVFSTDGRMGEACAERIRSI
                     VKNIEAGEIIIGRVVSTTNFGAFVNLKPGTDGLVHISKLGSGRRVETVEEVVQRGDVV
                     KVEVLDVAIQGGKEKISLRLIEKIGDDAQ"
     gene            6389..7669
                     /locus_tag="VFD74_05135"
     CDS             6389..7669
                     /locus_tag="VFD74_05135"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZK48967.1"
                     /translation="MAIDDHRLESLPNGQRLITERLDHLRSICLGFWIPVGSRDETGS
                     LTGTTHFIEHLLFKGSSRYSAERIAQVFDTLGGELNAATSKEYVVVYGKFVDEQLPAA
                     MDVLVDMLLTPTFCDLDNEREVVLEEIAMYEDAPQDLIHDQLMETVFGGHSLGHPVLG
                     TRKSMSEVTADQVKAYHSHHFRFSDMVVAAAGNVDHEGIRALLLDGHPKEDGNNLVRE
                     QVHPELTPRRSFLQKETEQMHVCLGGRGLPRGDPRRFVLSVLDSLLGGSLSSRLFQEV
                     REKRGLVYSIYSFTSMYCETGLVGVYFGCRPDRLAQVTETVDAELGRVAREPVPADEL
                     ERAKQHLKGRMLLGLESTNSRMTRLGKAVLMGTEILSLDEMTRRVEAVTAEQVLELAS
                     ELFDPAGLSVAGIGTEERFFEEAFASGTLGSSTR"
     gene            7776..8546
                     /gene="dapB"
                     /locus_tag="VFD74_05140"
     CDS             7776..8546
                     /gene="dapB"
                     /locus_tag="VFD74_05140"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZK48968.1"
                     /translation="MELIQVVVSGALGRMGSTVCNAVLAEPDLLLAGGADPAAYGAEG
                     AAVSVADLPFCAPSLEEALDLAEGGPVPEGVRGRVVVDFSRPDSVTANVLVCLRRGWP
                     AVVGTTGLSAEAIARLAIESDRTGTPALIAPNFAIGAVLMMEFARQAARHLDACEIVE
                     LHHEAKLDAPSGTARRTGDLIEAEWTDRGLEKSVPMHSVRLPGLVAHQEVIFGGLGQT
                     LTIRHDSLARESFMPGVILAVRRVAGLQGLVVGLENIL"
     gene            8598..10265
                     /locus_tag="VFD74_05145"
     CDS             8598..10265
                     /locus_tag="VFD74_05145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZK48969.1"
                     /translation="METDTRLKVIPLGGLGAIGKNMTVFEYGDDLVLIDAGLMFPDNE
                     MLGIDLVLPDFSYIVERRERLRAIILTHGHEDHVGALPFVLREMPEVPVYGTRLTLGL
                     VNSKLGEHNLQGKTTVHEINVGTSLTLGAFEVEFLEVCHSIPDGVGVALHTPVGVVVH
                     SGDFKLDQTPVDGRVTALQRFGELGQKGVLLFLSDSTNAETAGYTRAERSVGHNFDAI
                     FAQSEGRIIVASFASHIHRLQQAFDAAARVGRSVAVVGRSMIKNVNIAANLGYLTIPE
                     GTLIRPQDIPDIPLDSLVVLSTGSQGEPMSALARMAAHDHHLINIIPGDTVIISAKPV
                     PGNERSVHRTINRLFAAGAKVIYGAEAGVHVSGHAAAEELKILLNLVKPRYFVPVHGE
                     YRHLYAHAELARATGVEAENVFIMEAGDVLELGSDGARIAGRVQAGMVFVDGLAMGDF
                     QDVILRDRHHLATDGVAIVVITLSSQDGSIAAQPEVALRGFVHTGDMEDMVEQVKERV
                     VAALSSAKMQDIGDVSILKAHVHDEVQRFFKKEVKRRPLVLPILVEV"
     gene            10491..>10831
                     /locus_tag="VFD74_05150"
     CDS             10491..>10831
                     /locus_tag="VFD74_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48970.1"
                     /translation="MALFLSYVLVAGQTGGYFGEIGGAALTGVLGRLASLLPLGLVAV
                     AARLILQFKVEWRSVWVWGVVALVLGLTVLSAAGFPPLGPGHAVQTFERAAYETRAGW
                     TGEVTYAGLHRL"
BASE COUNT         1899 a         3284 c         3758 g         1890 t
ORIGIN      
        1 tcatcgaggc gtacgagatc aaggaagttg cccggaccag ctagcagccc gccgcgcggc
       61 ttcgtgggga tcatctccgc ggagctgcgc tttggggaag cacactcgct caaggacaag
      121 cgtgcgcaca tacgccgcgt ccgagaccgg ttgaccggtc agcacggagc cagtttcgct
      181 gagatcggat accaggatct gtggcagagg tcggccgtgg tcgtggcctt cgcggcgtcg
      241 gacctggcgg tcttggagcg gcggatggcg gcggcactcc ggttcctcga ttcgcaggag
      301 tgggagctgg tgtccgtgta tgaagaggtg gtggagatag atgagtgaga aacgctatcg
      361 gatgcagcgg gtcaacgaag cgctgcgcga ggtcttgagc atcgccatcg ggcgagggtt
      421 gaaggatccc cgtgtggggt tcgtcaccgt cacgggggtg gaagccacca acgacctgcg
      481 gcaggcccgt gtgtacgtct cgatactcgg agatgccgcc gagagggagc tgtcgttggt
      541 gggcttgcgc tcaggcgagg gcttccttca gggcgtcatc aacgacgagc tccagttgaa
      601 gcggacgccg gcgctcgagt tcgtctacga cgagagcgtc gatcggggtt tccgcatcag
      661 caagctcctg atcgccgaga ccgcactcct gggcgagccg atcgacgagc cgcagggtgt
      721 ggatgccgag cccggtgggg agacacccga gcggcctcat ccggtcggcc ccaacgagga
      781 cttgccgcct cagaccgaag agtcgtgaag agcatcacgc ctcgcgaggt cgctcgtcgg
      841 ctctcttccg agaaagagat cctgatcgtg agtcacgaga gcccggacgg agacgcactg
      901 ggctgcgtca cggcgctcgg cctgatggcc cgcaatcttc gcatcccgtt ccgcatgtac
      961 atccccggag agggatcctt tccgcccgaa tactccttct tgcctctgct ctccgatatc
     1021 gaacggggcg gcttccccga ggtcacgcga gagacaacgg cctatctgct ggattgcgcc
     1081 acggctgagc gcatcgatcc gacgggactc gcgtgtgcgg gcgactgcat caacatcgac
     1141 caccatcagg acaaccgcgg gtatgggacg ctgaacctgc tcgacttcgg cgcgacctcg
     1201 accacgcaga tcttgttcga cgtcttcgtg gcgggcggcc tgcctgtcga ttcggagatc
     1261 gccacctcgc tctacgtggg tttggtcacc gacagcggtc gcttccagta ttcgaacacg
     1321 acgccggccg cacaccggat ggcggccgac cttctgctgg ccggagtgga cgctaacgcg
     1381 gtcagtcgtg gactcttcga gacgatgcct ttggccaaga tactgttgca ggcgcgggca
     1441 ctggggcgcc tcgagatgcg cctgggtggc cgactggtca tttcgcgggt cgatgccgac
     1501 gacttcgcgg actccggcgc gagcgaaggg ctcaccgagg ggatcatcga cggtctgagg
     1561 cgcatcgagg gggtggccgt ggccgcgttc ctgcgcgagc gcacggtcgc cggcgtggtc
     1621 tcgcagaagg ttagccttcg ttccaccgac ggccgcatcg acgtggccga ggtggctcat
     1681 ctgcgcggcg gcggagggca cgtgcgggcc gcgggattca cggtggaggg tgaggcccgt
     1741 ggcgtcatgg actggatcga gcgcgaggtg ggtgcgcggt tgtgagcgtc ggcaccccgc
     1801 gcgccagggc cgagggcgtg tccagggtcg tcctcgtgga caagcccgcg gggtggacga
     1861 gttacgacgt cgtgaggcgc gccaaggggg cgttcaaggg caagatcggg cacgcgggca
     1921 cacttgaccc ttttgctacc gggctcctgg tggtgctctt cggtcaggcg acgcggcttt
     1981 cgtcggtttt catggatctg ccgaaggagt acgccgtcac cgtgcggttc ggggtcgtgt
     2041 ccagcacggg agacccgacg ggggagcttg tagccaccgg cggccgcacg gacctccctg
     2101 cgatcgtgtc cgctctcgac gcattccggg gcgagatcgt acaaagagtc cccatgacgt
     2161 ccgcggtcaa ggtcgagggc gagaggctct accagaaggc gcatcgagga gagacggtgg
     2221 agacgcccga gcgctctgtg cgcgtctatg acctcgcggt gagtgggttc gacgaggtcg
     2281 cgcaagacct cgagttgttg gccctgaccg gcaagggcac ctacgtgagg tgtctcgcgg
     2341 aagatctggg tgaggcgctt ggggtgggag cgcacgcggc ccgtctgcgg aggcggcgtg
     2401 tgggtgcatt cgacgtcgcc gacgccgtcg cgccgggcga tctccaaccg gacatgctgt
     2461 cgcagcccga cgggcgctcg gtgatgacgc tgtctgcagc gctctccttt cttccagcct
     2521 ttgaggtggg tgaggaagac gcgcgcctgg tgggaaacgg gggcaggctg tgcgatgctc
     2581 cgagcgggct gttccgcgtg tcgagcggcg ggaggctact ggcactctac gaaggatcgg
     2641 gagggagcgc gcagtccaga gtcgtgttcc cgacaccgga agactgaggc ggtgtccttg
     2701 tcgggtagtc tagatggggt ctgagtaggt gccccaaagg cagatgtaga tgcgtgtata
     2761 tcgtgacatt gatgaggtgg cggatcttgg tgggcggcct cgcgcggtgg cgatcggtac
     2821 cttcgatggt ctgcatcggg gccaccaaga gatcgtgaag agagctgtgc gggcggcggc
     2881 cgtcatggac ggggccgcga cggtgctcac cttcgagccg cacccgagtt ccgtcctggc
     2941 tccaggaagc gacccggcgt tgctgagcac gttcgagtac aaggtggatc tgctcgatcg
     3001 cgccggcgtg gatgaggtcg tggccctgcc gttcgatatg gctttcgcgg cactctcgcc
     3061 cgagcagttc tgtcgcagga tcctgtcgga gcgtttgaat gcccggcagg tgatggtggg
     3121 ggagaacttt cgattcggtc accaagcatc cggctccgcc gaagacctga tagcccacgg
     3181 tcggcggagt ggcttctcgg tcacggccat cggtctgatc atcgtgcgcg gaaagcccgt
     3241 ctccagcacc cgcatccgca gactcgtggc cgccggacgg gtggaagaag cggcggagct
     3301 gctcggtcgt ccccatgtca tcgaaggctt cgtcgggagc ggcgccgggc ggggccgctc
     3361 tctgggcact cccacggcga atctggaagc tgtcgccggg gtggcggttc cggccgcggg
     3421 ggtgtatgtc acccgcaccg tcgtcgccga cggagacgtg cgcccgtcgg tcacgagcat
     3481 cggcacgaat ccgaccttcg aatcagacgg gcgcgtgcgc atcgagacct tcgtcctcga
     3541 cttcaccggg aacctgtacg gcaggcgcat ccgggtcgag ttcctccagt ggatcagggg
     3601 acaagagacc ttcaccgatc ccgccgctct tcaagtgaag atagccgaag acgtgaacgc
     3661 agcgcgatcg ctgttcgggg aagtctccgg ccgcgcttga tcccagcctt ggttccgtga
     3721 ctgtagtctc ctttatggta accttccccg ggcgcctact tcggcgaccc gcatttacac
     3781 ctgcttctcg gccggatcga ttgaatcctc gctgggggca cggagcccaa ggaggctatg
     3841 catgctgagc aaggaacgga agacggacat catcgatcgc ttcaagaagc acgaaggcga
     3901 caccgggtcg cccgaagtgc agatcgcgat cctcaccgag cagatcaacg tgctcaccga
     3961 acacctcaag atccacaaga aggatcacca ctccaggcgg ggcctactca agatggtggg
     4021 tcgccgtcgc cgtctcctta actaccttca ggggcaggag atggagcggt accgcaccgt
     4081 ggtcaaggag ttgggactca ggaagtaagg gactttgagc gggcggcgcc acggggcgcc
     4141 gcccctcgtg catccggccc gcggggccgg atgccggtcc ttgtatcgag cgtcagccct
     4201 gggagggacc caggcgcgct agagcgaaag gcgaagatag agaatgacag agcaggcagc
     4261 ggcgaaccaa gcgagcacca cgacgatcca ggtgggcgac aagtccatcc tcctggagac
     4321 cggtcgtctg tccaaacagg ccgacggcgc ggtcctcgtg cgctccggag acacgatggt
     4381 gctggtcacc gcagtggttt ctaagaacga gcggaacgtt gatttcttcc ccctcacggt
     4441 cgacatcgaa gaggggatgt acgcggccgg gaagatcccg ggcggattca tcaagaaaga
     4501 gggacgggcg agcgagcagg ccattctgac tgcgcgtctc accgatcggc cgatcaggcc
     4561 gctgtggccg aagggcttca acaaggagac gcatatcgtc gccaccgttc tgtcggtgga
     4621 caagacgaat ccctacgacg tccttgccat cctaggcgcg tcggcggcgc tgagcatctc
     4681 gggcgcaccc ttccagggcc ccatcggggt cgtgcgggtg ggtcaggtgg acggtgcctg
     4741 ggtgatcaac ccgacgtacg ccgatcttga gtcgagcccg atcaacctga tcgtggccgg
     4801 cacggccgat gccatcagca tggtggaagc cggctgcgcc gaagtgagcg aagccgatgt
     4861 gctggctgct ctcgatgtgg ctcacgcggc catcaagatc caggtggccg ggatcgcgga
     4921 ctgggccggg caggttggca agccggccat ggaattcgtc gagcgtctgc ctgatcgtac
     4981 cctggtggag aggatcaagg ccggacacgc tagcgagatc ctgagtgccg tgtcggtcct
     5041 cgagaagcat gagaggtcgg cggcggtcgc ggctgtgaag caacaggttc tcgccgggct
     5101 ccagccggcc gatggctccg ttgacgcggc gactcacgcg gcggctgtgt cgaaggcctt
     5161 cgacgccgtc gagaaggaga tcgtgcgcaa gcgcatcgcc atcgacaagg tgcggcccga
     5221 cggcagggcc aaggacgaga tccgtcagat atcgtccgag gtgggcgtag ccccgcgcac
     5281 ccacggttcc gggctcttta cgcgcggtca gactcaggtc atgactctgc tcaccttggg
     5341 tgccagtcgc gacgagcagc gcatcgacgg catctccatc gacgaatcca agcagttcat
     5401 gcaccactac aagttccccc cgttctcggt gggagaagcc ggcttcatgc gcggccccgg
     5461 caggcgcgag atcggtcacg gcgccctcgc cgagcgtgct ctcgtaaggc tcgtccccga
     5521 gcatgaggac ttcccctaca cgatacgtct cgtctcggag acgctcgaat ccaacggatc
     5581 gagctccatg gccagcgtgt gcgcatcgac gctcgcgatg atggacgcgg gtgtgccgat
     5641 caagcgcccc gtggccggga tcgccatggg cctggtcaag gagatggatg agtacatcat
     5701 cctcaccgac atcgccggcg tggaagacca ccttggcgac atggatttca aggtggccgg
     5761 cacccgtgac ggcatcaccg cgctgcagat ggacatcaag atcaagggtg tgacgtccgc
     5821 gatcatgcgc gacgcactgg agcaggcctt gaaggcccgc ctcttcatcc tcgacaagat
     5881 ggccgaggtc atcgcgacgc cgcgcacgga gctcaaggag ttcgctcctc gcatcatcac
     5941 ggtgaaggtt gataacgaca agatcggcgc cgtcatcggc aagggcggcg agaccattcg
     6001 cggtctggaa gccgagttcg atgtgaccat cgagatcgac gatgagggtc tggcgaaggt
     6061 cttctccacc gacggtcgca tgggcgaagc ctgtgccgag cgcatccgga gcatcgtaaa
     6121 gaacatcgaa gccggcgaga tcatcatcgg cagggtggtg tccacgacga acttcggcgc
     6181 tttcgtgaac ctcaagccgg gcaccgacgg cctcgtccac atctcgaagt tgggctccgg
     6241 ccgtcgcgtc gagactgtcg aggaggtcgt ccagcgcggc gatgtggtga aggtggaagt
     6301 gctcgacgta gctatccagg gcggcaagga gaagatcagc cttcggctca tcgagaaaat
     6361 cggcgacgac gcccaatagg gcaagcgctt ggccatcgac gaccataggc ttgagagtct
     6421 gccgaacggc cagaggctca tcaccgagcg actcgaccac ctcaggtcga tctgcttggg
     6481 attctggata ccagtgggct cccgggacga gaccgggagc ctcaccggta ccacacactt
     6541 catcgagcac cttctcttca agggctcgag tcgatactcc gcggagcgga tagcgcaggt
     6601 attcgacact ctcggcggcg agctgaacgc ggctacgtcg aaggagtacg tcgtcgtata
     6661 cggcaagttc gtcgacgagc agctgccggc cgccatggac gttctggtgg acatgctgct
     6721 cactcccacc ttctgcgatc tcgacaacga acgtgaggtc gtgctcgaag agatcgcgat
     6781 gtacgaagac gcgccgcagg atctcatcca cgatcaactg atggagaccg tcttcggcgg
     6841 gcactcgctc ggtcatccgg tgctcggcac ccgcaagagc atgtccgagg tgaccgccga
     6901 ccaggtcaag gcttaccaca gccaccactt ccggttcagt gacatggtgg tcgcggccgc
     6961 cggcaacgtg gaccacgagg gcatccgtgc gctgctcttg gacggccacc ccaaggagga
     7021 cggcaacaac ctggtgcgtg agcaggtcca tcctgaattg acgccgcgcc gctccttcct
     7081 gcaaaaagag accgagcaga tgcacgtgtg cttgggaggc cggggtctgc cgaggggaga
     7141 cccgcgccgg ttcgtcctgt ccgttctcga cagcctgctg ggcggctccc tcagctcgcg
     7201 tctcttccaa gaggtgcgtg agaagcgggg cttggtctac agcatctact cgttcacgag
     7261 catgtactgc gagaccggcc tggtgggagt gtacttcggg tgtcgacccg accggctggc
     7321 gcaggtcacg gagacggtgg atgccgaact cgggcgtgtg gcgcgtgagc ctgtgcccgc
     7381 ggacgaactg gagagagcca agcagcacct gaagggtcgc atgcttctgg gtctcgagag
     7441 cacgaacagc cgcatgacgc gtctcggcaa ggcggtcctc atgggcaccg agatcctgag
     7501 tctggacgag atgacccgaa gggtggaggc ggtgacggcg gagcaggtgc tggaactggc
     7561 ctccgaactc ttcgatccgg ccggcctctc ggtcgccggc atcggcacag aagagagatt
     7621 cttcgaagaa gccttcgcga gcgggaccct gggaagttcc actcgctgaa tccggcgtgg
     7681 cggcgcgggc tctgctgtcc tctcgaggat gtactatcga gaggacttgg ctccgtgtgt
     7741 agcggtcgtc agttcgagaa ccaggggtct ggaccatgga acttatccaa gtagtcgtga
     7801 gcggcgctct cgggcggatg gggagcaccg tgtgcaatgc cgtgctggct gagcccgacc
     7861 tgctccttgc gggtggagcc gacccggccg cctatggggc cgagggcgcg gccgtcagcg
     7921 tcgcagacct tccgttctgc gccccgagtc tggaagaagc cttggatctg gccgagggcg
     7981 gcccggtgcc cgaaggcgtg cggggacggg tagtggtgga cttctcgcgg ccggacagcg
     8041 tgactgccaa cgtgctcgtg tgcctgagga gaggctggcc tgcagtggtc ggcaccaccg
     8101 ggctcagcgc ggaggcgatc gcacggttgg cgatcgagtc agaccgcacc ggcacgcccg
     8161 cgctgatcgc gccgaacttc gccataggcg cagtactgat gatggagttc gcccgccagg
     8221 cggctcggca tctcgacgca tgcgagatag tggaattgca tcacgaggcc aagctggacg
     8281 ccccctcggg cacagctcga cgcaccggcg acctcatcga agcagagtgg accgaccgcg
     8341 gtctcgagaa aagcgtcccg atgcacagcg tgcgccttcc ggggctcgtc gcgcatcaag
     8401 aggtcatatt cggcggtctg gggcagactc tcacgatcag acatgactct ctcgcccggg
     8461 agtcgttcat gccgggcgtc atcctggcgg tccgccgggt ggcgggtctg cagggactgg
     8521 tggtaggact ggagaacatc ttgtagatgc cggctccccg tcggagtgga gtcgcgcaca
     8581 ctggagggcc atacacgttg gagactgata cgagactcaa agtgatcccg ctcggtgggc
     8641 tcggagccat cggcaagaac atgaccgtct tcgaatacgg agacgatctg gtgttgatcg
     8701 acgcggggct catgttcccc gacaacgaga tgttgggcat cgatctcgtc ttgcccgact
     8761 tctcctacat agtcgagcgc cgcgaacgat tgcgcgccat catcctcact cacggccacg
     8821 aggaccacgt cggggcactt cccttcgtcc tgcgcgagat gcccgaggtg cccgtttacg
     8881 gcacgcgact tacccttggg ctcgtcaaca gcaagctggg ggagcacaac ctgcagggca
     8941 agaccacggt ccacgagatc aacgtcggca cgtcgctcac tttgggcgcc ttcgaggtgg
     9001 agttcttgga ggtctgccac agcatccccg acggcgtggg cgtggcgctg cacacgccgg
     9061 tgggtgtcgt ggtgcacagc ggcgacttca agctcgacca gactccggtc gatgggcggg
     9121 tcaccgcgct gcaacgcttc ggcgagttgg gccagaaggg cgtgctgctg ttcctgtccg
     9181 actcgacgaa cgcggagact gcgggataca cgcgggccga gcgctcggtg ggacacaact
     9241 tcgacgccat cttcgctcag tccgagggtc gcatcatcgt cgcatcgttc gcgagtcaca
     9301 tccatcgctt acaacaggct ttcgacgctg ccgcgcgcgt tggtcggtcg gtcgccgtgg
     9361 tggggcggag catgatcaag aacgtcaaca tcgccgccaa cctcggctac ctcaccatcc
     9421 ccgaaggcac gctcatccgt cctcaggaca tcccagatat cccgttggac agtctggtcg
     9481 tcctctccac cggaagtcag ggcgagccga tgtcggcatt ggcccgcatg gcggcgcacg
     9541 accaccatct gatcaacatc atccctgggg acaccgtgat catctcggct aagccggtcc
     9601 ccgggaacga gcgcagcgtg caccgcacga tcaaccgtct gttcgcggcg ggcgccaaag
     9661 tgatctacgg ggcggaggcc ggagtgcatg tctccggtca cgccgccgcc gaggagttga
     9721 agatcctgct gaacctggtg aagccgcgct acttcgtccc cgtgcacggc gagtaccggc
     9781 acctctacgc ccacgctgag ttggcgcggg ccacgggtgt ggaggcggag aacgtcttca
     9841 tcatggaagc cggcgacgtc ctcgagctcg ggtccgacgg tgcccgtatc gcaggtcgcg
     9901 tgcaggcggg catggtgttc gtcgacgggc ttgccatggg ggatttccag gatgtgatcc
     9961 tgagagaccg gcatcacctg gctacggacg gcgtggctat cgtggttatc accttgagtt
    10021 cgcaggacgg gtcgatcgcg gcgcaaccgg aagtcgccct gagagggttc gtccacaccg
    10081 gcgacatgga agacatggtg gagcaggtga aggagagggt ggtggccgct ctgtccagcg
    10141 cgaagatgca ggatatcggg gacgtctcca tcctcaaagc gcatgtgcac gacgaggtgc
    10201 agcggttctt caagaaggaa gtcaaacggc gcccgctcgt cctcccgatc ctggtggagg
    10261 tctgatcgag tgtgctgctg gacggggggc tgatggcggg tacgcgaggc ggcgatcggg
    10321 agccgacggc cgtgaggccg cggccaagat cgggcgccgc ctctaagaga ggcgaccgcg
    10381 gggggcgcag agaggcgccc gactccccga aggcacggcg gtcggagcct aagccaagca
    10441 ccacctgggg ccttcatcgg gcggagttga tcgccctggg gtgtctcggg gtggctctct
    10501 ttctgagcta cgtgctcgtc gccgggcaga ccgggggata cttcggtgag atcggcggag
    10561 cggcgctcac gggggtgctc ggccgcctcg cgtccctgtt gccgctgggg ctcgtggctg
    10621 tcgccgcgcg tctgatcctg cagttcaagg tggagtggcg atcggtatgg gtgtggggcg
    10681 tggtggcgct cgtcctgggc ctgacggtgc tctccgcagc cggattcccc ccgctcggcc
    10741 ccgggcacgc ggtgcagacc ttcgagaggg ccgcatacga gacgcgcgcg ggctggaccg
    10801 gggaagtgac gtacgccggc ctccatcggc t
//
LOCUS       DASRMJ010000187        12574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_195298, whole genome shotgun sequence.
ACCESSION   DASRMJ010000187 DASRMJ010000000
VERSION     DASRMJ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195298"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFD74_05155"
     CDS             <1..541
                     /locus_tag="VFD74_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HZK48971.1"
                     /translation="SYTLLDEFKELPQIKPLVEGGTLVEYSAHAIPEGGIAKLPKLFG
                     DGYLMVGDAAGLALNALLTVRGMDFAIASGFYAGLAVIAAKKQGDFSAAGLAQYEQSL
                     RSSFVLKDLETARHIPHTIENPRLFTHYPQAVSGLLEKLFTIGPQPAQKLSSTVVGGV
                     RKDFLNATTVKELWAMRKI"
     gene            554..829
                     /locus_tag="VFD74_05160"
     CDS             554..829
                     /locus_tag="VFD74_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZK48972.1"
                     /translation="MNIEAKLGLDVFKIDKERHITIDVEVCKAACTDKVCLYVCPAHL
                     YEIDDQGNNTVNYEGCLECGTCLISCDRGALSWNYPRGEYGVQYRMG"
     gene            930..1718
                     /locus_tag="VFD74_05165"
     CDS             930..1718
                     /locus_tag="VFD74_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZK48973.1"
                     /translation="MKLDIVVCVKTNPDLQMIRIKNREPVLEAIPFRVGDLDKNAVEA
                     AIQLRDAAGDGKVVVLAVAESNRKVRETVKEVLAMGADEAVLVVDPALEATDQAGVAR
                     ALAAAIQKIGSCDLVLFGEGSTDNYTGQVGSRVAELLALPQISYARKITAGGAGVLVE
                     RSMDESIETLEAPLPAAVTVVSEINEPRIPSLMNIMKAGKKPVEEWGLADLGLEAGTV
                     APVRTILSNLAEEQDRKHIMFEGSAADQASAFVNALIKEGVLGR"
     gene            1721..2677
                     /locus_tag="VFD74_05170"
     CDS             1721..2677
                     /locus_tag="VFD74_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZK48974.1"
                     /translation="MADILVYSEKMDAALELASKAKEFAGALGLGLSAAILGVDGTGA
                     ADAGRLGEVGADRVFTVFDPALDGLAVDVTAEALAQVAREAGATVVLVASTRRGKELA
                     PRLAQKLGAGCVTDVNSLVLDGATLVAGRYAFGGNTVAMETVDTGVKVFAVMPKTFEM
                     GPAVAGAGLVVSPALALQPSTVKLVERRQKDGDAVNLEDAERIVGIGRGLGKREDVDL
                     GRQLAAALGAELACTKGLSDFGWLAEDRVVGLSGAKTKPDLYLSVGISGQIQHTVGIS
                     SSKLIAAINTDKDAPIFQLADYGVVGDLYEVVPALIEKLNSI"
     gene            complement(2803..3363)
                     /locus_tag="VFD74_05175"
     CDS             complement(2803..3363)
                     /locus_tag="VFD74_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HZK48975.1"
                     /translation="MTSIVVLGVGNLLLTDEGVGPITIDYLGERWEFPGHVQLVDGAT
                     AGLELINVFQSADSIVVIDTVLGDAEPGALYRFHPDDVPRDVRYRTSIHQVSFMDAWN
                     MVRLLGPAPEIVIIGVQPEDMSTPHVGLTSTIEARLPDIEELVLGELERLGVPAPRRR
                     KPGAGAERAPDPYRLTPRYAPKAHEE"
     gene            complement(3360..4106)
                     /gene="tatC"
                     /locus_tag="VFD74_05180"
     CDS             complement(3360..4106)
                     /gene="tatC"
                     /locus_tag="VFD74_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZK48976.1"
                     /translation="MATIGEQKRMTVIDHLTELRRRIVVSVVAVALGMVVAFVEKDWV
                     FGIIKRPLLRMKGVNTDLVTFSPTEPFMTVLKVSIYAGMLLALPIVLWQIWAFIMPAL
                     YERERKSVLPYVFFTTALFVGGVAFAYYAVLPVGLGFLVGYGGDIFLQQLRASEYIGF
                     VSMFLLAFGVVFEMPAMMLLLTSAGIVDHHMLKRVRKYAVVGIAVAAMVLTPSQDPVS
                     MLLMMGPLLALYEIGILLSRASGRKRTARP"
     gene            4568..4936
                     /locus_tag="VFD74_05185"
     CDS             4568..4936
                     /locus_tag="VFD74_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZK48977.1"
                     /translation="MINPTVDLVIQFALMLFIGAFLGVALVTLSHFLAPTSKNPNKGL
                     PYECGVLPESDARAPYNVHFYLVAVLFVLFDLEAVFMYPWAVALHKLGTLGLVEMFLF
                     IFMLLVGYFYAWKKGVLEWE"
     gene            4927..5568
                     /gene="nuoB"
                     /locus_tag="VFD74_05190"
     CDS             4927..5568
                     /gene="nuoB"
                     /locus_tag="VFD74_05190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HZK48978.1"
                     /translation="MGMTDPTSIRQAAMLSPSGTAGLLSAADLAALRDASDAQVRQAL
                     DEDYIPGVAIPASLEKLLGFGRSFSWWPLLFGLACCAIEMMATAGPRYDLSRFGMEAM
                     RASPRQADGMIVAGWCSTKMAPRVVRLYEQIPDPKWVVAMGSCAISGNIWDTYNVVQG
                     IDALIPVDVYVPGCPPRPEALWDGLEMLRDRVKQSRHAYDGLRVIPARAAIGE"
     gene            5620..6150
                     /locus_tag="VFD74_05195"
     CDS             5620..6150
                     /locus_tag="VFD74_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZK48979.1"
                     /translation="METRQESVEIPEASKVVFAKLEEEFGGKLLGSSFFRGELTFTVS
                     VDDYVAVATFCRDSPDLAFERLDCLLGNHYPERLEAPFEVVLHLTSVSRNSRLRLKVS
                     LAEGQKIPTVSGLWRSADWDERETWEMYGIVFQGHPNLIRLLLTDDFEGYPLRKDFPV
                     KGTVGGRIRTDLKGKI"
     gene            6283..7425
                     /gene="nuoD"
                     /locus_tag="VFD74_05200"
     CDS             6283..7425
                     /gene="nuoD"
                     /locus_tag="VFD74_05200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HZK48980.1"
                     /translation="MGPQHPSMHGVYQAVVDLDGEIIVRTRPGIGNLHRGVEKLAENL
                     TYHQVIPLTDRLDYVSSFAMNHGYCEAVEKLMGVEVPVRARYIRTIAHELIRLSNHIL
                     WLGVHIMDFGAQTFFLICFRDREYILDLFEMACGARLTHSYARIGGVSKDLPDGWVGK
                     CRAVADFLPKRLAEYERLITVNRIFLKRARNVGIFTAEDCNQWRITGPALRAAGVARD
                     LRKDEPYAAYDEVDFEVAVEYDADVYARFLVRIKEMYESCKIIRQCLDNLPDGPFLAA
                     DAAHVLPLKKNVMRDSAAMVQDFVHVFRGPQVPKGEVYRSIEVPKGEMGVYIVSDGGP
                     KPQRLRLSTPSFYHGQAIPAMFEGEMLSDSVGIFGSVDIVLGDCDR"
     gene            7422..8426
                     /gene="nuoH"
                     /locus_tag="VFD74_05205"
     CDS             7422..8426
                     /gene="nuoH"
                     /locus_tag="VFD74_05205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZK48981.1"
                     /translation="MTDLIFLVVKGVLWVVVAVGMVPFIIYFERKFAGFFQSRVGPTY
                     AGPFGALQSFGDMAKLLSKEDLIPDWADKPVFRIAPYILFVPAFVTMALIPFGPMGVS
                     IFGHKVDLVIANFDAGAIMFLALGSIAVYGIVFAGWASNNHWSLLGSLRSGAQMVSYE
                     ISMGLALVGLFIMAGSVSFVGIVDAQAGNFWNWNFIPQIVGFAIFFVASIAELNRAPF
                     DLAEAEQELVAGHMTEYTGMRWGIFMFGEYVNMVVVSAVIATLFFGGWRPPFEALEFI
                     PGFIWLVGKVLFFVFMYMWIRWTLPRYRYNQLMDIGWKVFLPLALANLVVTALIVSVV
                     "
     gene            8428..8889
                     /gene="nuoI"
                     /locus_tag="VFD74_05210"
     CDS             8428..8889
                     /gene="nuoI"
                     /locus_tag="VFD74_05210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HZK48982.1"
                     /translation="MGLKSVIKKATFFEIFQGMSVTGGYFLKDKVTVEYPKEKIIPQP
                     RFRGMHALLTDPESGRLNCCGCRLCETICPSNCITIETSEQSNHKSIDVFDIDLARCI
                     FCGFCEEVCPCAAIVMTSVYELATYDKSDFYLTKERLVANQANAHKELYRR"
     gene            8900..9487
                     /locus_tag="VFD74_05215"
     CDS             8900..9487
                     /locus_tag="VFD74_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZK48983.1"
                     /translation="MADKFVFIMFAALAVIGALGVITRSNIVHGLILLVFTFLNVAGI
                     FLLTQAYFLAVIQVLVYAGAILVLFAFVVMFLNLREFQQLEQLHPKQKWVVLVLAPLM
                     LAEFIFVVTGATYASVGGGLSPEVIAERGGNVQVLGEVLFGEMLLPFEIASLILLVGM
                     VGAILLAHKEGGSENRLREWGEGEEPTSAFVEQEV"
     gene            9436..9795
                     /gene="nuoK"
                     /locus_tag="VFD74_05220"
     CDS             9436..9795
                     /gene="nuoK"
                     /locus_tag="VFD74_05220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZK48984.1"
                     /translation="MGRGRGADLSLRRAGGIAVQIPVEWYLVLSAFLFVIGATGVLVR
                     RNLFIVLFSIELMMNAVNLNLVAFSRLHENLIGQNFVLFTMTVAAAEAAVGLAILTSL
                     FRNRKTLMVDTFDLMKG"
     gene            9806..11716
                     /gene="nuoL"
                     /locus_tag="VFD74_05225"
     CDS             9806..11716
                     /gene="nuoL"
                     /locus_tag="VFD74_05225"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZK48985.1"
                     /translation="MENMNYVLPLIPALPLVAFVVTLLLGRWYIKDSAHWIPIFAMAG
                     SFVLSVWAFVAIRGAEHAFVVDLWRWIPVGDFQVNIGLQLDQLSSVMLLVVTGVGTLI
                     FIYSKGYMHGDTGYYRFFAFLSLFAFSMLMLVLGSNYLLLYFGWEAVGLCSYYLIGFY
                     YHKPAAASAGKKAFLTNRVGDFGFGLGVMLIFVTFGSLDYTKVFEAVELQQASQGVML
                     AIGLLLFMGAMGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVTAGVYMVARSSVIFA
                     SAPTAMLVVGGIGAFTAIFAATIGIAQNDIKRVMAYSTISQLGYMFMALGVGAWVAAI
                     FHLVTHAFFKALLFLGSGSVIHSLSGEQDMRKMGGLAKKIPFTYWTLMAGALALSGVF
                     PFAGFWSKDEILGMNWKGGGYVIWAIGLIAAFITAFYTFRLIFMTFWGKSRVDPEVEH
                     HVHESPWSMVVPLMVLGGLSVVGGFLGFPPEKGVFHKFLEPVFEPANKILGVHHEAFG
                     ATDLVLMVVSVAVALSGILVAYVFYVRRPDDLPQKAGAVAPPLYRAILNKWYIDEIYN
                     ATIIRLVVDGSRWLWRVVDAGVIDGAVNGVGRLWDIAGKAVSPLQTGRVQNYAVVMFL
                     GLFVIVSAVIIF"
     gene            11741..>12574
                     /locus_tag="VFD74_05230"
     CDS             11741..>12574
                     /locus_tag="VFD74_05230"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZK48986.1"
                     /translation="MDFGFADQIGFRALTLLTFLPALGALVMLLFMRGRPSAYKATAL
                     VTSLAAFALSAYMLTAFHTETAAFQFEENYVWIQNFGINYHVGVDGISMLMILLTTFL
                     SVIAIIGSWNYVKDRELAFFIGLLVLETGMIGVFTAMDLFLFYVFWEVQLIPMYFIIG
                     IWGGARRIYAAIKFFLFTFTGSVLMLVAILGIVFWFGRETGAMTFDILTLQKAGFPSN
                     IQFWGFLAFFVAFAIKVPMFPVHTWLPDAHVEAPTAGSVILAGVLLKMGVYGMIRFCI
                     GF"
BASE COUNT         2146 a         3916 c         4077 g         2435 t
ORIGIN      
        1 ctcctacacg ctcctcgacg agttcaaaga gctgccgcag atcaagcctt tggtcgaagg
       61 cggcacgctc gtcgaatact cggctcacgc catccccgag ggtggtatcg ccaagctgcc
      121 gaagctcttc ggcgacggct acctcatggt cggagacgcg gccggcctcg cgctgaacgc
      181 gctgttgacc gtgcgcggga tggacttcgc catcgcgtcc ggcttctacg ccggcctggc
      241 ggtgatcgcg gccaagaagc agggcgactt ctcggcggcc ggtctggcgc agtacgagca
      301 atcgcttcgc agcagcttcg tcctcaagga cttggagacc gcgcggcaca tcccgcacac
      361 catcgagaac ccgcgtctct tcacgcatta cccgcaggcc gtgtcgggtc tgctcgagaa
      421 gctgttcacc atcggccctc agcctgccca gaagctgtcg agcaccgtcg tgggcggggt
      481 ccgcaaggat ttcctgaacg cgaccacggt caaagagctc tgggcgatgc gaaagatcta
      541 gggaggggcc acgatgaata tcgaagccaa actggggctc gacgtcttca agatagacaa
      601 ggagcggcac atcacgatcg acgtcgaggt gtgtaaggcg gcgtgcaccg acaaggtctg
      661 cctatacgtg tgcccggccc acctgtacga gatcgacgac cagggcaaca acaccgtgaa
      721 ctacgagggc tgcctcgagt gcggcacctg tctgatctcc tgcgaccgcg gtgcgctgag
      781 ctggaactac cctcgaggtg agtacggggt gcagtaccgc atggggtagc ccctggggcg
      841 ggcgggcgcg acttccgtcg gcgcgcccgc aacgacgccc tcgagctgca caagctgctg
      901 taacactcgg gttctacgag agaagggtga tgaagttgga catcgtcgtg tgtgtgaaga
      961 cgaacccgga tctgcagatg atccggatca agaaccgaga gccggtcctg gaagcgatcc
     1021 ctttcagagt gggcgatctc gacaagaacg cggtcgaggc tgcgatccaa ctgcgcgacg
     1081 ccgccggcga cggcaaggtc gtggtgctcg ccgtggccga gtcgaaccgc aaggtgcgcg
     1141 agacggtcaa ggaagtcctg gccatgggtg ccgacgaggc ggtcctggtg gtggacccgg
     1201 cgttggaggc caccgatcag gcgggcgtgg ctcgggctct cgccgccgcg atccagaaga
     1261 tcggctcgtg cgatctggtg ctcttcggtg aaggatccac cgacaactac accggtcagg
     1321 tgggctctcg agtagctgaa ctcttggcac ttcctcagat cagctacgcc cgcaagatca
     1381 cggcgggcgg cgcaggcgtg ctcgtggagc gcagtatgga cgagtccatc gagactctgg
     1441 aggctccttt gcccgcggcc gtcaccgtgg ttagcgagat caacgaaccc cgcataccgt
     1501 cgctgatgaa catcatgaag gccggcaaga agccggtgga ggagtggggc ctcgccgatc
     1561 tgggcctgga ggcgggaacg gtcgcgccgg tgcgcaccat cctctccaac ttggcggaag
     1621 aacaggaccg caagcacatc atgttcgaag gcagcgccgc cgaccaggct tcggcgttcg
     1681 tgaacgcgct catcaaagaa ggggttctgg ggagatgacc atggccgata tcctcgtata
     1741 ttcagagaag atggacgcgg ccctggagct cgcttccaag gccaaggagt ttgcgggcgc
     1801 gttgggactt gggctctccg cggcgatcct cggagtcgat gggaccggcg cggccgacgc
     1861 gggcagactg ggtgaggtcg gtgccgatcg cgtcttcact gttttcgacc cggccctgga
     1921 cggcctcgcc gtggacgtga cggccgaggc cctggcccag gtggcccgcg aggccggtgc
     1981 gactgtggtg ttggtggcct cgacccggcg gggcaaggag ttggcgccgc gtctcgcgca
     2041 gaagctcggc gcgggctgtg tcacggacgt gaactcccta gtgctggacg gtgccaccct
     2101 ggtggccggt cggtacgcat tcggcggcaa cacagtggcg atggagaccg tcgacaccgg
     2161 ggtcaaggtg ttcgcggtca tgcccaagac gttcgagatg ggccccgcgg tcgccggcgc
     2221 gggcctggtc gtgtcgccgg ctctcgccct gcagccgtcc accgtgaagc tggtggagcg
     2281 tcgccagaag gacggcgacg ccgtcaacct cgaagacgcg gagcgcatcg tgggcatcgg
     2341 tcgtggtctg ggcaagcgtg aggatgtgga tctgggcagg cagctggctg ccgcactggg
     2401 tgcggaactc gcctgcacca agggtctgtc cgacttcggg tggttggccg aagaccgcgt
     2461 cgtcggcctc tccggggcca agaccaagcc cgacctctac ctgtctgtgg gcatctccgg
     2521 ccagatccag cacacggtgg gcatcagctc gtcgaagctc atcgccgcca tcaacaccga
     2581 caaggacgcc cccatcttcc agctggccga ctacggtgtc gtgggggacc tctacgaggt
     2641 cgtcccggcc ctgatcgaga agctgaacag catctgacga acggtctgtt ccgtccgggc
     2701 ggcctccggc cgcccggacg gcagtagctc cctcggcggc gagagacggc gcctccgagg
     2761 gcttcggccg gggtcgcgca cgcgcgaccc cggcccacca actcactcct cgtgcgcctt
     2821 cggcgcgtac cgcggcgtga ggcggtaagg gtcgggcgcc cgctcggcgc cggcacccgg
     2881 cttgcggcgc ctcggcgccg gcacgcccag ccgctcgagc tcccccaaca ccagctcttc
     2941 gatgtcgggt agcctcgcct cgatggtgga ggtgagcccc acgtggggag tggacatgtc
     3001 ctcgggctgc acaccgatga tgactatctc gggggccggg cccaacagcc ggaccatgtt
     3061 ccaggcatcc atgaacgaca cctgatggat cgaggtgcga taacgcacgt cccgggggac
     3121 gtcatcgggg tggaagcggt agagggcgcc cggttcggcg tccccgagga ccgtgtcgat
     3181 cacgacgata ctgtcggccg actggaacac gttgatcagt tccaggccgg cggtcgcacc
     3241 gtccaccaat tgcacgtgac cggggaactc ccagcgctcg cccaggtagt cgatggtgat
     3301 gggacccacg ccctcatctg tgagcagcag gttgcccaca ccgaggacga cgatggacgt
     3361 cacgggcgcg cggtccgttt tcggccggag gcgcgcgaca gtaggatgcc gatctcgtag
     3421 agtgccagga gcggacccat catgaggagc atgctgaccg ggtcctggct gggggtgagc
     3481 accatcgccg cgaccgcgat gcccacgacc gcgtacttgc gtacgcgctt cagcatgtgg
     3541 tggtcgacga tcccagcgga cgtgagcaac agcatcatgg ccggcatctc gaacacgact
     3601 ccgaacgcca ggaggaacat cgagacgaac ccgatgtact cggatgcccg cagttgttgt
     3661 aggaagatgt cgcctccgta ccccaccagg aagccgagac ccaccggcag cacggcgtag
     3721 taggcgaagg cgaccccgcc gacgaagaga gcggtggtga agaatacgta ggggagcacg
     3781 ctcttgcgct cgcgctcgta gagcgcgggc atgatgaacg cccagatctg ccagaggacg
     3841 ataggtagcg ccagcaacat gcccgcgtag atggatacct tgagcacggt catgaaaggt
     3901 tcggtggggc tgaaggtaac caggtcggtg ttgacaccct tcatgcgcaa caggggccgc
     3961 ttgatgatgc cgaacaccca gtccttctcg acgaacgcga cgaccatacc cagggcgaca
     4021 gccaccaccg acaccacgat gcgccgacgc aactcggtga gatggtcgat cacggtcatg
     4081 cgcttctgtt cgccaatcgt agccaacgcg tgctcctcgg gggccgatcg gggtgccgaa
     4141 cgcccggagt cttctgcctt cgtcggcggt gcggcgaaca caagcgggcg tgccggctag
     4201 tatagcaacg gtgtgtgccc gacggcggct acggtgtgcg cgcgatggcg cagatcgccc
     4261 gcgtcgtcga tctgccggga tgccgcatgc gggcggttcg catggctgcg cgacggtggg
     4321 cgaggtaagc ggcgatcggc ctcatccgac gctccccggc aaatggccgg gaacgggaca
     4381 tctgctcacg ggaacggccg caatcggggg ttgagcggcc cactccacct gtgaaatccg
     4441 ttgcaaccac cgcgccgatt tgctattgtg acccccgttg tgtgagtcct cacacacttg
     4501 tgcgcgagga ctcaggtact cgagctacgg aacgtcgcct aggcaaccga accgcagagg
     4561 tgcgcctgtg atcaatccga ctgtcgacct cgtcatacag ttcgccctga tgctcttcat
     4621 cggagccttc ctcggggtgg ccctcgtgac gctcagtcac ttcctggctc cgacgagcaa
     4681 gaacccgaac aaaggccttc cctacgagtg cggggtgctg cccgaaagcg atgcccgcgc
     4741 tccgtacaac gtgcacttct atctcgtcgc cgtcctcttc gtgctgttcg acctagaggc
     4801 ggtgttcatg tatccctggg ctgtcgcgct gcacaagctc ggcaccttgg gactggtcga
     4861 gatgttcctg ttcatcttca tgctcctggt gggctacttc tacgcgtgga agaagggagt
     4921 gctcgagtgg gaatgaccga tccgacgagt attcggcagg cagccatgct ctcgccctcc
     4981 gggaccgccg ggttgctgtc agcggccgac ctagccgccc ttcgcgatgc ttccgacgcc
     5041 caagtcagac aggctcttga cgaggactac atccccggag tcgccatccc cgcaagcctc
     5101 gagaagctcc ttggtttcgg ccgtagcttc tcctggtggc ctctcctgtt cgggctggcc
     5161 tgctgcgcta tcgagatgat ggcgaccgcc ggcccccgat acgacctctc gcgctttggg
     5221 atggaagcga tgcgtgcgtc tccccggcag gccgacggca tgatcgtggc cgggtggtgc
     5281 agcaccaaga tggcccctcg cgtcgtgcgt ctctacgagc agatccccga ccccaagtgg
     5341 gtggtggcca tggggtcgtg cgccatctcg ggcaacatct gggacacgta caatgtggtg
     5401 cagggcatag acgcactgat ccccgtggac gtgtacgttc ccggatgccc tccgcggccg
     5461 gaggcgctgt gggacggtct cgagatgctc cgcgaccggg tcaagcagag tcgccatgcc
     5521 tacgatggtc ttcgggtcat cccggcgcgc gcggcgatcg gcgaatagcg gacgggatac
     5581 ggtcaagagc ccggcggccc gatccggaga aggggaagga tggaaacgag acaagagagt
     5641 gtcgagatcc cggaggcgtc gaaggtcgtc ttcgccaagc tcgaggagga gttcggaggc
     5701 aagttgctcg gctcctcttt tttccgcgga gagctgacgt tcaccgtatc cgtggacgac
     5761 tacgtcgcgg tggcgacgtt ctgcagggac tctcctgacc tggccttcga acgcctcgac
     5821 tgcctactgg gcaatcacta ccctgagcgg ctggaagctc ccttcgaggt ggtcctgcac
     5881 ctgacctccg tgagccgcaa ctcgcgtctg cgcctgaagg tgtcgttggc cgaagggcag
     5941 aagatcccca cggtgtccgg cctgtggcgt tccgccgact gggacgagcg ggagacctgg
     6001 gagatgtacg gcatcgtgtt ccagggccac ccgaacctca tccgtctgct cctcaccgac
     6061 gacttcgagg ggtatccgct gcgcaaggac ttcccggtca agggcaccgt gggcggccgg
     6121 atcaggaccg atctgaaagg aaagatctag atgagcgggc acggctcgga tctgcacgac
     6181 gcgcccgtgg ctccgcagca cccgacactc gaggagctca cagagcgcaa ggcccgcacg
     6241 cgccagcgcg tggcggccaa caacgagacg atcaccgtca gcatgggccc gcagcacccc
     6301 tcgatgcacg gggtgtacca agcagtggtg gacctcgacg gtgagatcat cgtccgcacc
     6361 cgcccgggta tcggcaacct gcaccgcggc gtggagaagc tagccgagaa cctgacctat
     6421 caccaggtca taccgctcac cgaccgtttg gactacgtct ccagtttcgc gatgaaccac
     6481 ggctactgcg aggcggtcga gaagttgatg ggggtcgagg tccccgtcag ggcgcgctac
     6541 atcaggacca tagcccacga actgatccgc ctctccaacc acatcctgtg gctgggcgtt
     6601 cacatcatgg acttcggggc gcagaccttc ttcctcatct gcttccgcga ccgcgagtac
     6661 atcctcgacc tgttcgagat ggcgtgcgga gcgcgactca cgcactccta cgcgcgtatc
     6721 ggcggcgtct ccaaagacct gcccgacggt tgggtaggga agtgtcgggc ggtagccgac
     6781 ttcctgccga agcgtctagc cgagtacgaa cggctcatca ccgtcaaccg catcttcttg
     6841 aaacgcgcgc ggaacgtggg catcttcacg gcggaggact gcaaccagtg gcgcatcacg
     6901 ggacctgcac tgcgcgccgc cggcgtcgcc cgcgacctac gcaaggacga gccctacgcg
     6961 gcctacgacg aggtcgattt cgaggtggcg gtggagtacg acgccgacgt gtacgcccgc
     7021 ttcctggtgc gcatcaaaga gatgtacgag tcgtgcaaga tcatccggca gtgtctggac
     7081 aatctgcccg acggcccgtt cctggccgcg gacgccgccc acgtgctgcc gctcaagaag
     7141 aacgtgatgc gcgattcggc ggccatggtg caggacttcg tgcacgtgtt ccgcggaccg
     7201 caggtgccga aaggtgaggt ctaccggtcc atcgaggtgc ccaagggcga gatgggcgtg
     7261 tacatcgtct ccgacggcgg gccgaaaccc caacgtctgc gcctgtccac tcccagcttc
     7321 tatcacgggc aggccatccc cgccatgttc gagggcgaga tgctctcaga cagcgtgggg
     7381 atcttcggta gcgtcgacat cgtactgggg gattgcgacc gatgaccgat ctcatcttcc
     7441 tcgttgtcaa gggcgtcctc tgggtcgtgg tggccgtcgg gatggttccg ttcatcatct
     7501 acttcgagcg caagttcgcg ggcttcttcc agagccgcgt aggccccacg tatgccggtc
     7561 ccttcggggc gctgcagtcg ttcggtgaca tggccaagct gctcagcaag gaagacctga
     7621 tccccgactg ggccgacaag cctgtgttcc ggatcgcccc ctacatcctg ttcgtgcccg
     7681 cgttcgtcac catggcgctg atcccgttcg gccctatggg cgtgagcatc ttcggacaca
     7741 aggtcgacct ggtgatcgcg aacttcgatg cgggcgccat catgttcctg gcgctgggct
     7801 ccatagctgt gtacggcatc gtgttcgccg gttgggcctc gaacaaccat tggtcgctgc
     7861 ttggcagcct gcgctccggc gctcagatgg tctcctacga gatcagcatg ggcctggcgc
     7921 ttgtgggtct gttcatcatg gccgggtcgg tgagcttcgt cggcatcgtc gacgcgcagg
     7981 ccggcaactt ctggaactgg aacttcatcc cgcagatcgt gggttttgcc atattcttcg
     8041 tggccagtat cgctgaactc aaccgggcgc cgttcgactt ggccgaggcc gagcaggagt
     8101 tggtggccgg ccacatgacg gagtacaccg gtatgcgctg gggcatcttc atgttcggcg
     8161 agtacgtgaa catggtggtg gtctcagccg tcatcgccac gctcttcttc ggcggttggc
     8221 gaccgccctt cgaggcgctc gagttcatcc ccggatttat ctggctggtg ggcaaggtcc
     8281 tgttcttcgt gttcatgtat atgtggatcc gctggacact gccccgctat cgctacaacc
     8341 aactcatgga tatcgggtgg aaggtcttcc ttccactggc cctcgccaat ctggtcgtca
     8401 cggccctgat agtgagcgtg gtgtagcatg ggtctgaaga gcgtcatcaa gaaggccacc
     8461 ttcttcgaga tcttccaagg gatgtccgtc accgggggtt acttcctcaa ggacaaggta
     8521 acggtggagt atcccaagga gaagatcatc ccgcagcccc ggttccgggg catgcacgcc
     8581 ctgctcaccg atccggaatc cggcagactt aactgctgcg ggtgtcggct gtgcgagacc
     8641 atctgcccct cgaactgcat caccatcgag acctcggagc agagcaacca caagagtatc
     8701 gacgtcttcg acatcgacct cgcccgctgc atcttctgcg gcttctgcga ggaggtctgc
     8761 ccgtgcgcgg ccatcgtgat gacgagcgtg tacgagctgg ccacctacga caagagtgac
     8821 ttctacctca ccaaggagag actggtggcc aatcaggcaa acgctcataa agagctctac
     8881 cggcgctagg ggagggagca tggccgacaa gttcgtcttc atcatgttcg ctgccctggc
     8941 ggtgatagga gccctgggcg tcatcacgcg ctccaacatc gtccacgggc tcatcctgct
     9001 cgtgttcacg ttcctgaacg tggccggcat cttcctgttg acccaggcct acttcctggc
     9061 cgtgatacag gtgctggtgt atgccggagc catcctggtg ctgttcgcgt tcgtggtcat
     9121 gttcctgaac ctgcgggagt tccagcagct cgagcagctg caccccaagc agaagtgggt
     9181 ggtgctcgtg cttgcgcccc tcatgctggc cgagttcatc ttcgtggtga ctggggccac
     9241 ctacgcctcc gtgggagggg gactgtcgcc cgaggtgatc gccgaacgcg gtggcaacgt
     9301 gcaggtcttg ggcgaagtgc tgttcggcga gatgttgctg cccttcgaga ttgcgtcgct
     9361 gatactcctc gtgggcatgg tgggagcgat actgctggcc cacaaagagg gcggaagtga
     9421 gaaccggttg cgggaatggg gcgagggcga ggagccgacc tcagccttcg tagagcagga
     9481 ggtatagcgg tgcagatacc ggtcgaatgg taccttgtgc tctccgcctt cctcttcgtc
     9541 atcggagcca caggggtgct cgtgcgtagg aacctgttca tcgtgctctt ctccatcgag
     9601 ttgatgatga acgcggtcaa cctcaacctt gtggccttca gccggctgca cgagaacctc
     9661 atcgggcaga acttcgtgtt gttcaccatg acggtggccg ccgcggaggc ggccgtgggc
     9721 ctggcgatcc tgacatccct gttccgcaac cggaaaacgc tgatggtcga caccttcgac
     9781 ctcatgaagg gctaggtgta ggcgcatgga gaacatgaac tacgtcttgc cactgatccc
     9841 cgctctgccg ctggtggcct tcgtggtgac cctgctgctg ggccgttggt acatcaagga
     9901 ctccgcgcac tggatcccta tcttcgcgat ggccggttct tttgtgctgt cggtgtgggc
     9961 gttcgtagct attcggggcg cggagcacgc cttcgtggta gatctgtggc gctggatccc
    10021 ggtgggcgac ttccaggtga acatcgggtt gcagctggat cagctgtcgt ctgtgatgct
    10081 cttggtggtc accggggtgg gcacgctcat cttcatctac agcaaaggct acatgcacgg
    10141 cgacacgggc tactatcgct tcttcgcctt cctcagcctc ttcgccttca gcatgctcat
    10201 gctggtgctg ggaagcaact acttgctgct ctacttcggc tgggaggcgg tggggctctg
    10261 ctcctactat ctcatcggct tctactacca caagccggcg gcggcctctg ccggcaagaa
    10321 ggcgtttctc accaacaggg tgggcgactt cggcttcggc ctcggggtca tgctgatctt
    10381 cgtgaccttc gggtcgctcg actacaccaa ggtcttcgag gcggtggaac tccagcaggc
    10441 gagtcagggc gtgatgctgg ccatcggcct cctgctcttc atgggagcca tgggcaagtc
    10501 ggcccagttc ccgctgcacg tgtggcttcc cgatgccatg gaaggcccca cgccggtgtc
    10561 cgccctcatc catgccgcca ccatggtcac cgccggtgtc tatatggtgg cccgcagttc
    10621 tgtgatcttc gcgagtgcgc ccacagccat gctggtggtg gggggcatcg gcgccttcac
    10681 ggccattttc gccgccacca taggtatcgc gcagaacgac atcaagcgcg tcatggcgta
    10741 ctcaacgatc tcccagttgg gttacatgtt catggccctg ggcgtgggcg cgtgggtggc
    10801 ggccatcttc cacctggtga cgcacgcctt cttcaaggcc ctgctcttcc tcggctccgg
    10861 ctcggtgatc cactcgctct cgggcgagca ggacatgcgg aagatgggcg gtttggccaa
    10921 gaagataccc ttcacctact ggaccttgat ggccggcgct cttgccttgt ccggggtgtt
    10981 ccccttcgcc ggcttctggt cgaaggacga gattctgggc atgaactgga agggcggcgg
    11041 ctatgtgatc tgggccatcg gcctgatcgc ggccttcatc accgccttct acaccttccg
    11101 cctcatcttc atgaccttct ggggcaagtc gcgcgtcgat cccgaagtgg agcaccacgt
    11161 gcacgagagc ccgtggtcga tggtggtgcc gctgatggtg ctgggagggc tgtcggtcgt
    11221 gggcggattc ctcggcttcc ctcccgagaa gggtgtgttc cacaagttcc tcgagcccgt
    11281 gttcgagcct gcgaacaaga tcctcggtgt gcaccacgag gcgttcggag cgacggacct
    11341 ggttctgatg gtcgtgtcgg tggccgtggc cctgtcgggg atactcgtcg cctacgtgtt
    11401 ctatgtgagg cgtcctgacg atctgccgca gaaggcgggc gccgtcgcgc cgccgctgta
    11461 cagggccatt ctgaacaagt ggtatatcga cgagatctac aacgcgacca tcatccgctt
    11521 ggtcgtggac ggttcccggt ggctctggcg ggtggtcgac gccggcgtca tcgacggggc
    11581 ggtcaacggg gtcggacggc tctgggatat cgccgggaag gcggtcagcc ccctgcagac
    11641 cggacgcgtg cagaactacg cggtcgtcat gttcctgggg ctgttcgtca tcgtcagtgc
    11701 ggtcataatc ttctagagcg ccaacagagg gagtgagaca gtggatttcg gtttcgctga
    11761 ccagataggc ttcagggcgc tcacgctgct gacgtttctg cctgcgttgg gcgccttggt
    11821 catgctgctg ttcatgcggg gtcggccgag cgcgtacaag gccacggccc tggtgacgag
    11881 tctggccgcc ttcgcgctga gcgcctacat gctgaccgcc ttccacacgg agacggcggc
    11941 cttccagttc gaagagaact acgtgtggat ccagaacttc ggcatcaact accacgtggg
    12001 tgtggacggc atctcgatgc tgatgatcct gctcacgacg ttcttgtcgg tgatcgccat
    12061 catcggctcg tggaactacg tgaaggacag ggaactcgcc ttcttcatcg gcctgcttgt
    12121 gttggagaca ggcatgatcg gcgtcttcac ggccatggac ctcttcctgt tctacgtgtt
    12181 ctgggaagtc cagctgatcc cgatgtactt catcatcggc atctggggag gcgcgcggcg
    12241 catctatgcc gccatcaagt tcttcctctt cacgttcacg ggctcggtgc tcatgctggt
    12301 ggctatcctg ggcatcgtct tctggttcgg acgcgagacc ggcgccatga ccttcgacat
    12361 cctgacgctg cagaaggcgg gcttcccctc gaacatccag ttctggggct tcctggcctt
    12421 cttcgtggcc tttgccatca aagtgcccat gtttcccgtg cacacgtggc tccccgatgc
    12481 ccacgtcgaa gctcccaccg ccggctcggt gatcctggcc ggcgtgctcc tgaagatggg
    12541 cgtctacggc atgatccgct tctgcatcgg gttc
//
LOCUS       DASRMJ010000188         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_134194, whole genome shotgun sequence.
ACCESSION   DASRMJ010000188 DASRMJ010000000
VERSION     DASRMJ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134194"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..309
                     /locus_tag="VFD74_05235"
                     /pseudo
     CDS             111..309
                     /locus_tag="VFD74_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165908.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            431..>2875
                     /locus_tag="VFD74_05240"
     CDS             431..>2875
                     /locus_tag="VFD74_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK48987.1"
                     /translation="MISEDSGRLHPDRRPSRPTQGEGAGATRPEGDDRCRALVEQCAD
                     LIAATDDSGIITFASSASQSLFGCPPQDMCGRPFTELLDDPDVPTALASFRAWSEGGE
                     PARNLRLRMKRTDGSLFFGELSGSQLGGERNGAMVIIRDVTEREEIENALRRAAEDLR
                     AAQRLAKVGSWNWTLEPDTVVWSKELCRINGHDPGLPAPSFSEMSSFYTPEGWTRLNE
                     AVAKAFDHGEPYELDLDLVREGGSMRSTIARGAADYDADGNLTGLHGTVQDITERKEM
                     ENALRESEERVRHLIESAHDWVWEIDEHGRYVFASPRLVDVLGYEPAEVLGRTPFDLM
                     PPDEAERVASLFTAIAAKRESFRDLPNTNLHKDGHLVHLETSGVPIFDSAGAYRGYRG
                     TERDITARRQAEASLRFTQFSVDHALDAIYWTDPDGRVVRVSDSACRLFGYSEQELLA
                     MSVLDLDPSLSAEQRAANSERLRKANVLTFETIGRTKSGDIFPLEVTVNRVDYQGTEY
                     NCVFAHDITTRRQVERERLELERRLQHSQKLESLGVLAGGIAHDFNNILTSVLGNAEL
                     ALSELSPSAPARENLLEITQASHRAAALCRQMLAYSGRGKFVSEPIDLSVFIEDLLPL
                     LKSSISKKALLNLRLEKNLPLLEGDPSQLGQVVMNLVLNASEALGEEDGAITIRTGAQ
                     ECSRDYLRESFADRDLPSGLYLTLEVSDTGCGMDAETQSHLFEPFYTTKFTGRGLGLT
                     AVLGIVRGHKGALRLESEPGRGTTFEILLPASQAPREMLPGPTDDTTSHWRGEGTVLL
                     VDDEETIRAMGKRMLLR"
BASE COUNT          556 a          977 c          894 g          448 t
ORIGIN      
        1 ttcctgtact cggaggacct acagcacgga cgccggttcg gcggcctccg catcgagaat
       61 ccgttcttgg ccgaacagcc gatcgacgct gcggagggtg cgcccgcccc ctgaccaaga
      121 ccgccctgat taccggcatc accggccggg acggcgccta cctggccgaa ctcccgccgg
      181 gcaagggctt acactgtgta cagcatcaag cgccggacct caagcttcaa cacacaaagg
      241 gtcgaccacc tctaccacga accccacgag caggacgtgc gcttcttcat gcactacggc
      301 gacctcaccg gcggacccgc gcaggtggtc cgcagcccga cacgacgcac ccacgacggg
      361 ttgcgcgaga ggcgcacggg gtagagaatg cggcgtagaa tgcagagtca ccttccgcac
      421 aggaggctac gtgatcagcg aggactccgg ccggctccac ccggatagac ggccttcgag
      481 acccacgcag ggagaagggg cgggagcgac gcggcccgag ggcgacgaca ggtgccgcgc
      541 gctggtggag caatgcgccg acctcattgc cgctaccgac gacagcggca tcatcacgtt
      601 cgcctcgtcc gcttcccagt ctctcttcgg atgcccaccc caagatatgt gcggccgccc
      661 tttcaccgag ttgctcgacg accccgacgt gcccacggcg cttgcctcgt tccgcgcctg
      721 gagcgagggg ggcgaaccgg ccaggaacct ccgattgagg atgaagcgga ccgacggctc
      781 tctcttcttc ggagaactca gcggttccca gctcggcggc gagcggaacg gtgccatggt
      841 catcatccgc gacgtcaccg agcgcgagga gatagagaac gccctgcgcc gggccgcgga
      901 ggaccttcga gcggcgcagc ggctggccaa ggtcggcagc tggaattgga cgctggaacc
      961 cgacaccgtt gtctggtcca aggaactctg caggatcaac ggccacgatc ccggactccc
     1021 cgcgcccagc ttctccgaga tgtcgtcgtt ctacacgccc gaggggtgga cccgactgaa
     1081 cgaggcggta gccaaggcgt tcgaccacgg agagccctac gaactcgatc tggatctcgt
     1141 gcgcgagggc ggcagcatga ggagcacgat cgccaggggt gccgcagact atgacgccga
     1201 cggcaacctg accggcctgc acggcacagt gcaggacatc acggagcgca aggagatgga
     1261 gaacgccctg cgcgagagcg aggagcgggt ccgccacttg atcgaatcgg ctcacgactg
     1321 ggtctgggag atagatgaac acggccgcta tgtgttcgcc agcccaagac tagtcgatgt
     1381 gctgggctac gagcccgcag aggtgctcgg aaggaccccc ttcgacctca tgcctcccga
     1441 tgaagctgag cgtgtcgcct ctctcttcac agcgatcgca gccaagaggg aatccttccg
     1501 cgacctgccg aacaccaatc tgcataaaga cggacatctt gtccacctag agaccagcgg
     1561 ggtgcccatt ttcgattcgg ccggcgccta ccgcggctac cgcggcacgg aacgcgacat
     1621 caccgcacgc aggcaggcgg aggcgtcgct tcgattcacg cagttctcag tggaccacgc
     1681 cctcgatgcg atctactgga cggaccccga cggccgcgtc gttcgcgtga gtgactcggc
     1741 ctgtcgactc ttcggctact ccgagcaaga gctcttggcc atgagcgttc tcgatttgga
     1801 cccgtctctc tccgccgaac agcgggccgc caactcggag aggctacgga aagcgaacgt
     1861 tctcaccttc gagactatag ggcgcacgaa gagtggcgac atcttcccgc tcgaggtcac
     1921 cgtcaatcgc gtcgactacc agggcacgga gtacaactgc gtgttcgccc acgacatcac
     1981 cactcgcagg caggttgagc gcgaacgcct ggagttggag cgccgactcc aacactccca
     2041 gaagctggag agcctgggag tcctcgccgg cggtatcgcc cacgacttca acaacatcct
     2101 caccagtgtg ctcggtaacg ccgagttggc cctctcagaa ctctctccct cggctcccgc
     2161 ccgagagaac ctcttggaga tcacccaagc ctcccaccgg gcggcggcgc tctgccggca
     2221 gatgctggcc tactccggtc ggggcaagtt cgtcagcgag cccatagacc tgagcgtgtt
     2281 catcgaggac ctgctgccct tgctgaagag cagcatctcc aagaaggctc tgctcaacct
     2341 gcggttggag aagaacctgc cccttctcga aggcgacccc agccagctcg gccaggtggt
     2401 catgaacctg gtgctgaacg cctccgaggc gctgggggaa gaggacgggg ccatcaccat
     2461 ccgcaccggg gcgcaggagt gctcccgcga ttatctgcgt gagtccttcg cggatcggga
     2521 tctaccctcg ggcctctacc tcaccctgga ggtgtccgac accggctgcg gaatggacgc
     2581 ggagacgcag agccatctct tcgagccctt ctacaccacc aagttcaccg gacggggctt
     2641 ggggttgacg gccgtccttg gaatcgtgcg cggccacaag ggagccctgc ggttggagag
     2701 cgagcccggc cgaggcacca ccttcgagat ccttctccca gcgtcgcaag cccccaggga
     2761 gatgctcccg ggcccgacgg acgacaccac ctcacactgg cggggagagg gtaccgtcct
     2821 tctggtggac gacgaggaga ccatccgggc catgggcaaa cgcatgctcc tccgc
//
LOCUS       DASRMJ010000189         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_134767, whole genome shotgun sequence.
ACCESSION   DASRMJ010000189 DASRMJ010000000
VERSION     DASRMJ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134767"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFD74_05245"
     CDS             <1..251
                     /locus_tag="VFD74_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZK48988.1"
                     /translation="AVAGEVVKAYVVLHRNVSPTDDLRLELIGFARKRLGAAIAPRDV
                     AFAEHLPHTQSGKIVRRVLKQRALGLPEGDTSTLEAAT"
     gene            248..1243
                     /gene="pdhA"
                     /locus_tag="VFD74_05250"
     CDS             248..1243
                     /gene="pdhA"
                     /locus_tag="VFD74_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HZK48989.1"
                     /translation="MSVPGPAGDLDVEGRRHLLVDMLRIRRFEERCVELYSASEIRGF
                     LHLSIGEEACAVGVMRALGPHDAIVSTYREHGHALARGLSMRSIMAELEGKVDGCSRG
                     RGGSMHLFDVDARFYGGNAIVAGGLPLAVGLALADHLTARQQVTACFFGDGAVAEGEF
                     HESMNLAALWHLPVLFCCENNLYAMGTSLSLTESETDLSLKAAAYRMPSWEVDGMDVL
                     AVADAAREAAASVRAGGGPHFLELRTYRFRAHSMYDPDRYRDKSEIEAWRHRDPITLL
                     GDALRASGELDDSALERIERDIAGEIDDAVAFAAASGIEPVEDLERFVTTIGAER"
     gene            1240..>2136
                     /locus_tag="VFD74_05255"
     CDS             1240..>2136
                     /locus_tag="VFD74_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HZK48990.1"
                     /translation="MSACTYREAVREAIRQAMLADERVFCMGEDIGHYGGCYAVTMGL
                     LEEFGSERIRDTPLSESAFVGAGIGAALGGQRPIVEVMTVNFSLLALDQILNNAATLR
                     YMSGGQLSVPLVIRMATGAGRQVAAQHSHSLEGWYAHIPGLRVLVPATIEDARGMLQP
                     ALDDPDPVIIFEHAGLYNEKGDLPDDPKTVAIEGAVARREGQDVTLVAYGGTVRVALG
                     AAEELAAAGVSAEVVDLRCLRPLDSATVAASVTKTHRVVVLDEGWRTGSFSAEVASRV
                     SEECFYELDAPPARVCTAEVPMP"
BASE COUNT          358 a          672 c          715 g          391 t
ORIGIN      
        1 atgcggtcgc gggggaggtt gtgaaggcct acgtagtgct tcaccggaac gtgagcccga
       61 ccgatgacct ccgcctcgag ttgatcgggt tcgccagaaa gcgtctcggt gcagccattg
      121 ctccccggga cgttgcgttc gccgaacacc tgccgcacac tcagagcggg aagatcgttc
      181 gaagggtgct caaacagcgc gccctgggcc ttcccgaagg tgatacgtcg acactcgagg
      241 ccgccacatg agcgtgccag ggccggccgg cgatctcgac gtggagggtc ggcgccatct
      301 tctagtcgac atgctccgaa ttcgccggtt cgaggaacga tgcgtcgagc tttacagcgc
      361 ctcggagatc cgaggattcc tccacctgag tatcggggaa gaggcttgcg ccgttggcgt
      421 catgcgggcg ctcggacccc acgacgccat tgtttccacc taccgtgagc acgggcatgc
      481 cctcgcccgg ggactgtcca tgcgctcgat catggccgaa ctcgaaggca aggtcgacgg
      541 ctgcagccgg ggccgtggtg gttcgatgca cctgttcgac gtggatgctc gcttctatgg
      601 tggcaacgcc atcgtggcgg gtggcctacc gctcgccgtc ggtcttgctc ttgccgatca
      661 cctcaccgca cgccagcagg tgaccgcctg cttcttcggg gacggcgcgg tggccgaggg
      721 ggagtttcac gagtcgatga acctcgctgc actctggcac ctacctgtct tgttctgctg
      781 cgagaacaat ctgtacgcca tgggaacctc cctctcgctc accgagtcgg agaccgatct
      841 ctcgctcaag gctgctgcat accgcatgcc gtcttgggaa gtcgatggca tggacgtgtt
      901 ggcggtggca gatgctgcac gagaagccgc ggcgagcgtg cgcgccgggg gagggccgca
      961 cttcttggag ctgcggacct accgtttccg tgcgcactcc atgtatgacc cagaccgata
     1021 tcgcgacaag tcggagatcg aagcatggcg gcaccgtgac ccgatcacgc tccttgggga
     1081 cgcacttcgg gcttctggtg agctcgatga cagtgcgctc gagaggatcg agcgggacat
     1141 cgccggcgag atcgatgacg cggtcgcctt tgccgcggcc tctggcatcg aacctgtgga
     1201 ggacctcgag cgcttcgtaa cgacgatcgg ggctgagcga tgagcgcgtg cacgtatcgt
     1261 gaggccgtcc gcgaggcgat ccggcaagcc atgctcgccg atgagcgggt cttttgcatg
     1321 ggggaagaca tcggtcacta cggggggtgc tacgccgtca ccatggggct gctcgaggag
     1381 ttcgggtccg agcgcatccg tgacacgcca ctgtcggagt ccgcgttcgt tggcgcagga
     1441 atcggggcag cgctcggggg acaacggccg atcgtcgaag tgatgacggt caatttcagt
     1501 ttgcttgccc tcgatcagat cctgaacaac gcggcaacac tgcgctacat gtccggaggt
     1561 cagttgagcg tgccactcgt gatccgcatg gcgactggcg cgggacgcca ggtggccgcc
     1621 caacactcgc acagcctcga gggatggtac gcgcacatcc cgggccttcg agtacttgtc
     1681 cccgcgacca tcgaggacgc ccgcggcatg ctccaacccg cgctcgacga ccctgatccg
     1741 gtcatcattt tcgaacacgc cggcctgtac aacgagaagg gggaccttcc cgacgacccc
     1801 aagacagtag ccatagaggg tgcagtggct cgccgggagg gtcaggacgt cacgctcgtt
     1861 gcctacgggg gaaccgttcg tgtcgccctc ggtgctgcag aggagctggc agcggcgggc
     1921 gtctcggccg aagtcgtgga cctccgctgc ctgcggcctc tcgactctgc gactgtggct
     1981 gcgtcggtga cgaaaaccca ccgcgttgtc gtgctcgacg aaggctggcg cacgggaagc
     2041 ttttccgccg aggtggcgag ccgcgtcagc gaggaatgct tctacgagct cgatgcgccg
     2101 cccgctcgcg tgtgcacggc cgaggtcccc atgccc
//
LOCUS       DASRMJ010000190         5054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_16379, whole genome shotgun sequence.
ACCESSION   DASRMJ010000190 DASRMJ010000000
VERSION     DASRMJ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16379"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFD74_05260"
     CDS             complement(<1..735)
                     /locus_tag="VFD74_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZK48991.1"
                     /translation="MAPSTQPLSSYAGGLDHLLAVVNVAVMAIDGRGRPTLLNDEAAR
                     LLRVQPPPPSEIAHALRAVSWLAHEVKNPLATIQLYPELIGRTLPPDDRQLVTTIKEQ
                     VNLAQTRISELLRSLSPRVEDSHAPLFTDLGLAAADFVARKAPALPHVRLSTEIAAGP
                     HNVPLRPDDALSLLQHLVTNASQAVEGPGTTAVRVESGLGLTRLTVEDSGPGFPAEDP
                     ERLLQPFFTTKRRSPGLGLWAVKRICD"
     gene            904..2085
                     /locus_tag="VFD74_05265"
     CDS             904..2085
                     /locus_tag="VFD74_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZK48992.1"
                     /translation="MMVPQEHAAAGFGVDFGAERVLIVDDEPGIRRYLGRVISAAGRE
                     VDEFENPDEALSQLRSGDYAVALVDIKMPGHDGLWLLGQVAGSDLDVCIVMVTGDTDV
                     HTAVSCLTRGADDYITKPVDKAQLTQTVAKAVQTRRLRIENKAYREDLERLVGERTAQ
                     LEGALQDVRKGGAALAQAYRDSIYRLASAAEYRDSETGGHIRRIGLYSHVIAQHLGCD
                     DDFLELVRLASPMHDVGKIGIRDSILLKPGRLTPEEFEEMKAHTLIGGRLLGGSDYPL
                     MCLAETIAVSHHERFDGSGYPHGLAGENIPLAGRIVAIADVFDALITQRVYKPAFTWE
                     RALEILHEESDGHFDPRVLSAFDKGLSLITKVIVADTDPAISGARRYRTATSLSIAKP
                     D"
     gene            2211..3242
                     /gene="hemE"
                     /locus_tag="VFD74_05270"
     CDS             2211..3242
                     /gene="hemE"
                     /locus_tag="VFD74_05270"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZK48993.1"
                     /translation="MTSEYRFLKACRREDVDATPVWMMRQAGRYLEEYRALRAQHSFL
                     EMCRTPELAAEVTIQPLRRFDIDAAIIFADILLPLPGIGVDLEFAKGEGPVIGNPVRS
                     AADVDALTPLVPEQHVPFLAEAIKMVRREIEGRVPLIGFSGAPFTLASYVIEGGSSRN
                     YVNTKQMMYGAPAVWHRFMDKMAGVVSTYLDYQIDAGAQVVQLFDSWVGVLSPADYRE
                     YVLPYSQKVIESIGGVVPVIHFGTDTATLLPLMKEAGGDVIGVDWRMDLADAWRVLGD
                     DVGVQGNLDPVVLYAPPAVIEEKVAEVLRSAAGRPGHIFNLGHGILPTTPIEHARVMI
                     DAVHRLSAR"
     gene            3275..4375
                     /locus_tag="VFD74_05275"
     CDS             3275..4375
                     /locus_tag="VFD74_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZK48994.1"
                     /translation="MLAYGGPDSLDEVEPFLRRIMAPREPSEETIARATERYRAIGGR
                     SPLVGNTLLQARLLQERLDAAGVALGLGGRGVSFHVVPGMRHTAPSIHDAVRRAVTGS
                     DGTIVALIMASHQSERATGAYLREVSASFEGLSAEEGSGAAAPLWVDTWHTSALFVEA
                     VAERVREALARLDSGPKAVTAAGDANPAGVGADDADRADAASVIDDANHAAAADDDTA
                     VLFTAHSLPLVNGEGDPEYERALLATAEGVMSLVGDRPWRLAYQSASAARGGRWLGPR
                     VEDSLRELATVGLRKVVVAPLGFVSEHLETLYDLDIDLAGEALEAGVVMTRAATVHDS
                     PKFIEALAEAVVACVRGRGVQRGKGRPVKVRA"
     gene            4372..>5054
                     /gene="hemG"
                     /locus_tag="VFD74_05280"
     CDS             4372..>5054
                     /gene="hemG"
                     /locus_tag="VFD74_05280"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZK48995.1"
                     /translation="MRTVPARIVVVGGGVGGMAAAWEARREAERLGAPVSITVVESRA
                     RVGGSVVTERVGDCLVEGGPDCFVSEKAGGLQLVRDLGLEAHLSPTNDNERKTFIMWK
                     RRLHPLPDGLILLVPTRILPFVTATLFSWPGKLRMAMDLVIPRRREETDETLGDFIRR
                     RLGTEVLAKLGEPLVAGIHSGDPETMSVRATFPRFLDMEREDRSLIVAMLKRMKQARA
                     ARAAAQKARA"
BASE COUNT          838 a         1561 c         1799 g          856 t
ORIGIN      
        1 atcgcagatc ctcttgaccg cccataggcc gaggcccggg ctgcgtctct tggtagtgaa
       61 gaagggttgg agaaggcgct cggggtcttc tgcggggaag cccgggccgg aatcttcgac
      121 ggtgagtctg gtgaggccga gtccggactc gacccggacg gcagtcgtcc cggggccctc
      181 cacggcctgc gaggcgttcg tgacgagatg ctgcaagagt gaaagggcat catccgggcg
      241 cagaggcacg ttgtgggggc cggccgcgat ctccgtcgat agccgcacgt gcgggagcgc
      301 cggtgccttg cgcgccacga agtccgcggc cgccaggccg aggtcggtga agagaggggc
      361 gtgcgaatcc tcgactcgag gcgagaggga gcgcaggagc tcggagatcc gggtctgcgc
      421 caggttcacc tgctccttga tcgtggtgac cagttgtcgg tcgtcggggg ggagagtgcg
      481 gccgatgagt tcggggtaga gctggatagt cgccaggggg ttcttcacct cgtgggcgag
      541 ccacgagacg gctcgaagag cgtgggcgat ttcgctcggg ggcggcggct gcaccctgag
      601 cagacgcgcg gcctcgtcgt tgagcagggt agggcggccc cggccgtcga tagccatgac
      661 ggcgacgttc accacggcga gcaggtgatc gagaccgccc gcgtaacttg aaaggggctg
      721 agtgctgggg gccatggatg gccatcgata ctaacacacc gcacatgcgg gacatcgccc
      781 gccccgctac cggtgctggg gaagatatgc agacctgtgc ttcgggaaca cgccgtgcga
      841 gcctgtaccg ccgacccgca ggatggtatc ttcacacggc gcgacactgg gtagaccggg
      901 aggatgatgg tgccgcaaga acatgcagcg gccgggttcg gagtagactt cggggccgag
      961 cgcgtcttga tcgtcgatga tgagccgggt atcagaaggt atctgggccg cgtgatctcg
     1021 gctgcgggtc gcgaggtcga cgagttcgag aatccggatg aggccctttc gcagctgcgg
     1081 agcggcgact acgccgtcgc cttggtggac atcaagatgc ctggacatga cggtctgtgg
     1141 ctgctcggcc aagtggccgg ttccgatttg gatgtgtgca tcgtcatggt gacgggggac
     1201 acggatgtcc acaccgccgt ctcctgcttg acccggggcg cggacgacta catcaccaag
     1261 ccggtcgaca aggcccaact cacccaaaca gtcgccaaag ccgtccaaac caggcgtctg
     1321 cgcatcgaaa acaaggccta ccgcgaagat ctggagcgcc tggtgggcga gcgaaccgcc
     1381 caactggaag gtgccctgca ggacgtgcgc aagggcggcg cggctcttgc gcaggcgtat
     1441 cgagacagca tctaccgtct tgcgtccgcg gctgagtatc gggattccga gacgggcggg
     1501 cacatccggc gcataggtct ctacagccac gtgatcgccc agcacctggg gtgcgacgat
     1561 gacttcctgg agctcgtgcg cctagccagc cccatgcacg acgtgggcaa gatcggcata
     1621 cgtgactcca tcctgctcaa gccgggccgt ctgacccccg aggagttcga ggagatgaag
     1681 gcgcataccc tgatcggcgg ccgcctgttg ggcgggtccg attaccctct catgtgtctt
     1741 gcggagacca tcgcggtgag tcatcacgaa cgcttcgacg gcagcggcta cccgcatggg
     1801 ttggcgggag agaatatccc gcttgccggt cggatcgtgg cgatcgcgga tgtgttcgac
     1861 gcactgatca cgcaacgcgt gtacaaaccg gccttcacct gggagagggc gctggagatc
     1921 ctgcacgaag agtccgacgg acacttcgat cctcgggtgc tcagcgcttt tgataagggt
     1981 ctgagcttga tcacgaaggt catcgtagcg gacacagatc cggccatatc cggtgcgcgt
     2041 cgctaccgga ccgcgacctc cctctcgatc gcgaagcctg actgagccga agagtgccgc
     2101 tcgcccctgg gtgtcacccg tcggcattcg gtgcccttcc tggtatattc tacggcccgc
     2161 gtacgaccca attgggcccg cctcgccggg cgcgagacga aaggaatccc atgacatccg
     2221 agtaccggtt cttgaaagcc tgtcgccggg aagacgtcga cgcaaccccg gtgtggatga
     2281 tgcggcaggc cggccgctac ttggaagagt accgggctct tcgggctcag cactcgttcc
     2341 tcgagatgtg ccgcacccct gagttggccg ctgaggtcac catccagccg ctgcgccgtt
     2401 tcgacatcga tgcggccatc atcttcgccg acatcctctt gccgctcccc gggataggcg
     2461 tggatctgga gttcgccaaa ggggaaggtc cggtcatcgg gaatcccgtc aggtccgccg
     2521 ccgacgtcga tgcgctcact ccgctcgtgc cggaacagca cgtacccttc cttgccgaag
     2581 ccatcaagat ggtccggagg gagatcgagg ggcgagtccc cctcatcggg ttctcggggg
     2641 cgcccttcac tctcgccagc tacgtcatcg aaggcgggag ttcgcgcaac tacgtgaaca
     2701 ccaagcagat gatgtacgga gctcccgccg tgtggcaccg tttcatggac aagatggccg
     2761 gcgtcgtcag cacctacctc gattatcaga tcgacgccgg cgcccaggtg gtgcagctct
     2821 tcgatagctg ggtgggcgtg ctcagcccgg cggactaccg cgagtatgtg ctgccctaca
     2881 gccagaaggt gatcgagtcc atcgggggcg tggtgccggt gatccacttc ggtacggaca
     2941 ccgcgacgct ccttccgctg atgaaggagg ccggcggcga cgtcatcggc gtcgactggc
     3001 gcatggacct ggcggatgcg tggcgtgttc ttggcgatga cgtgggcgtg cagggcaacc
     3061 tggacccggt cgttctctac gcgccgcccg ctgtcatcga agagaaggtg gctgaggttc
     3121 tgcggtcggc ggcggggcgc cccggtcaca tctttaatct gggccacggc atcctcccga
     3181 cgacgccgat cgaacacgcc cgagtgatga tcgacgctgt gcatcggctg agcgcgcgct
     3241 agtgggggga acgcctgaga aggtcgcggt gctgatgctg gcgtacggcg ggcccgactc
     3301 tctcgacgag gtggagccgt tcttgcggcg catcatggcg ccgcgggagc cttcggaaga
     3361 gaccatcgcg cgcgccactg agcgttatag ggcgatcggc ggtcgctccc ctttggtcgg
     3421 caacacactc ttgcaggccc ggctgctgca ggagcggctc gacgcggctg gtgtcgcact
     3481 cggactcggc ggcaggggcg tgagcttcca tgtggtgccc gggatgcggc acacggcccc
     3541 gtccatccac gacgcagtgc gacgggccgt cacggggtcg gacggcacca tcgtggctct
     3601 gatcatggcc tcgcaccagt cggagcgggc cacgggcgcc tacctgcgcg aggtatccgc
     3661 atcgttcgag gggctctcgg ctgaagaggg ctccggagcg gccgcgcctc tgtgggtgga
     3721 cacctggcat acgtcggcgt tgtttgtcga agcggtggct gagcgtgttc gcgaggccct
     3781 ggcgcggctg gattccggcc ctaaggccgt gaccgccgcc ggcgacgcca accccgccgg
     3841 cgtgggtgcc gacgacgccg accgcgcgga tgcggcctcg gttatcgacg acgccaacca
     3901 tgcagccgcg gccgacgacg acacggccgt cctgttcacg gcccacagcc ttcccctggt
     3961 gaacggcgaa ggcgaccccg agtacgagcg cgcgctgctg gctacggccg agggcgtcat
     4021 gtcactggtg ggcgaccggc cctggaggct cgcgtaccag agcgcgagtg cggcccgcgg
     4081 aggacgttgg ttggggccgc gcgtcgaaga ctctctgcga gaactcgcga ccgtgggcct
     4141 gcggaaggtg gtcgtagcgc ctctcgggtt cgtgtccgag cacttggaga ccctctacga
     4201 cctggatatc gatcttgccg gcgaggctct cgaggcgggg gtggtgatga cacgcgcggc
     4261 tacagtgcac gactctccga agttcatcga ggccctcgcg gaggcggtag tggcctgcgt
     4321 gcgagggcgt ggcgtgcagc ggggtaaggg tcgccctgtg aaagtgcgag catgagaacg
     4381 gtgccggcga ggatcgtcgt cgtcggaggt ggcgtcggag gcatggcggc cgcgtgggaa
     4441 gcgcgtcggg aggccgagcg cctgggggcc cccgtctcca tcaccgtggt tgagagccgc
     4501 gctcgcgtgg gaggcagtgt ggtcaccgag agagtcgggg actgcctggt ggaagggggg
     4561 cccgactgtt tcgtgagcga gaaggccgga ggactccaac tggtgcgtga tctcggcctc
     4621 gaggcgcacc tgtcgcccac caacgacaac gagcgcaaga ccttcatcat gtggaagcgc
     4681 cggctgcatc cgctgcccga cgggctcatc ctgctggttc ctacgcgcat cctgccgttt
     4741 gtgacggcca ctctgttctc ctggccgggg aagctgcgca tggcgatgga cctggtcatc
     4801 ccccggcgcc gagaagagac ggacgagacg ctcggggatt tcatccgccg ccggttgggc
     4861 acggaggtgc tcgcgaagct gggagagccg ctggtggcgg gcatacacag cggcgatccc
     4921 gagaccatga gcgtgcgggc cacattcccc cgtttcctcg acatggaacg ggaagaccgg
     4981 agcctcatcg tcgccatgct gaagaggatg aagcaggccc gcgccgcgcg ggccgcggcg
     5041 cagaaggctc gcgc
//
LOCUS       DASRMJ010000191         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_196569, whole genome shotgun sequence.
ACCESSION   DASRMJ010000191 DASRMJ010000000
VERSION     DASRMJ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196569"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFD74_05285"
     CDS             complement(<1..293)
                     /locus_tag="VFD74_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48996.1"
                     /translation="MEFPRPRQPREWQEISFEYWPDPLAGPWLVKIGWRQISGRWEPI
                     GIKAEATEPPGPVPLESHILTAKLVREIPVGELVKSGRRKQQATCEMVAGWEE"
     gene            388..648
                     /locus_tag="VFD74_05290"
     CDS             388..648
                     /locus_tag="VFD74_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK48997.1"
                     /translation="MNADLKQEVPTNRELLEVSRMRDMVRGGLVKEIRLNAALSLREV
                     AEVVGVTPSAVFYWEQGTNIPRGKPAVRYARLLLELESLARR"
     gene            645..863
                     /locus_tag="VFD74_05295"
     CDS             645..863
                     /locus_tag="VFD74_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZK48998.1"
                     /translation="MTPQELQALRQSTVTTIELASHALSIGRTLGYQLARERGELAEG
                     VRVLRVGRKLVVPTRPLLAVLGYGEADE"
     gene            860..1084
                     /locus_tag="VFD74_05300"
     CDS             860..1084
                     /locus_tag="VFD74_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK48999.1"
                     /translation="MSAAATDLKQVIGVLKATDKGFHDGTITLVVSKRLGSVVGYRIQ
                     TQEPDELTRAQREIDRLMRERPQAERDVYL"
     gene            1081..2406
                     /locus_tag="VFD74_05305"
     CDS             1081..2406
                     /locus_tag="VFD74_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HZK49000.1"
                     /translation="MSAQLHFDEAAELAVLGCLLTPSANGARDPGGLRPEDFYKPTHG
                     LIFRACRDLAAAGEPVDALTVSNRLQKAGRLEEVGGRAFVHSLADALYPVTNLPTYAG
                     IVRDHARRREEVRIGQALEAGELTAEEAGRALEALAPVLIDTPELSDAFIDWSVFWER
                     DRTQADWIYEDLLARGRGHALYAVHKGGKSLLMLSVSVQLATGPEPIVVIYLDYEMGE
                     ADIYERLEDMGYGPGSDLSRLRYALLPSLPPLDTAAGARALTALVDAVQGEWPEHHLV
                     VILDTMGRAVAGEENSSDTFRAFYTHTGIELKRRGITYARLDHGGKDIAKGQRGSSGK
                     GDDVDVVWRLTKTEGGIRLDLDVARMNWVPKKVTFGIHEDPLRYVQVDSAYPVGTTDT
                     AELLDNLEVPLEASVRTAQEALKEAGEGRRQQVIRAALKWRREHQECAS"
BASE COUNT          483 a          797 c          773 g          405 t
ORIGIN      
        1 ttctcttccc acccggcaac catctcgcac gtcgcctgct gcttgcgtcg cccgctcttc
       61 acgagttcgc ccacaggaat ctcgcgcacc agcttggcag tcaggatgtg cgattccagg
      121 gggacaggcc cgggtggctc ggttgcctcc gccttgatac cgattggctc ccagcgaccg
      181 cttatttgtc gccagccgat tttcactagc cacggccccg ccagcggatc gggccagtat
      241 tcgaagctta tctcttgcca ttcgcggggc tgtcgcggtc ttggaaactc catcgcgctc
      301 ctctcataca ccccccagac ctatcaagaa cacatacaaa gataatgggt gcttgtgggg
      361 gggttatcag tgtgtcatta tcatcgcatg aacgcggact taaagcaaga ggtgcccacg
      421 aatcgagaac tccttgaggt ctcgaggatg cgcgacatgg tgcgcggtgg cctggtgaag
      481 gaaatccgac tcaacgcggc cctcagcctt cgcgaggttg ccgaagtggt aggcgtgacg
      541 ccgagcgcgg tgttctactg ggaacaggga acaaacatcc ccaggggcaa gcccgccgtg
      601 cggtatgcgc gactcctcct cgaactcgaa agcctcgcgc gccgatgacc ccgcaagaac
      661 tccaagcgct gcggcagagc accgtgacta cgatcgagct tgcgtcgcac gcgctctcga
      721 ttgggcgcac gctcgggtat caactcgcac gtgaacgcgg ggagctggcc gaaggcgtgc
      781 gggtcctccg cgtggggcgc aagctggtag tgccgacccg gcccctgctg gccgtgctcg
      841 gctacgggga agcggacgaa tgagcgccgc ggccaccgat ctgaagcagg tcattggcgt
      901 gctcaaggcg accgataaag gcttccacga cgggacaatc acgttggtcg ttagcaagcg
      961 tctcgggtcc gtcgttggct accggattca gacgcaagaa cccgacgaac tcacgcgggc
     1021 acaacgcgag atcgatcgcc tcatgcgtga gcgcccccag gcagagaggg acgtgtattt
     1081 gtgagtgccc aactccactt cgacgaagcc gcggaactgg ccgtactagg ttgcctgctc
     1141 acaccttccg ccaacggtgc ccgagacccg ggcggcctcc gacctgagga cttctacaag
     1201 ccgacgcacg gattgatctt ccgagcctgc cgggaccttg ccgcggccgg ggagccggtg
     1261 gacgcactca cggtatcgaa ccgcctacag aaggcaggca gactcgagga agtgggcggg
     1321 cgggccttcg tgcacagtct ggccgacgcg ctctacccgg tgacaaacct gccgacctac
     1381 gcgggcatcg tccgcgacca cgcccgacgc cgcgaagagg tgcgcatagg ccaggcgctc
     1441 gaggcgggag aactgaccgc agaggaggca gggcgcgcgc tcgaggctct cgcacctgta
     1501 ttgatcgata cacctgagct gagcgacgct ttcatagact ggtcggtctt ctgggaacgg
     1561 gaccgcactc aggcggactg gatctacgaa gacctactag cccggggccg gggccacgca
     1621 ctctacgccg tccacaaggg cggcaagagc cttctgatgc tctccgtatc ggtccagctc
     1681 gccacggggc ccgagcccat cgtggtgatc tatctcgact atgagatggg cgaggccgac
     1741 atctacgagc gcctcgagga tatgggctac gggcccggct cggatctctc ccgcctacgc
     1801 tacgcgctgc taccgtccct accgccgctc gacaccgccg ccggcgcccg agcgctcacg
     1861 gcactcgtgg acgctgtaca aggcgagtgg cccgagcatc acctggtggt gatccttgac
     1921 accatggggc gggctgtcgc tggtgaggag aacagctccg acaccttccg cgccttctac
     1981 acccacacag ggatcgaact caagcgccgc ggcatcacct acgctcggct cgaccacggc
     2041 ggcaaggaca tagccaaggg ccagcgcggc agctccggca agggcgacga cgtagacgtg
     2101 gtgtggaggc tcaccaagac cgagggcggc atccgcctcg atctcgacgt tgctcgaatg
     2161 aactgggtgc ccaagaaggt gaccttcggg attcacgaag acccgctccg atacgtgcag
     2221 gtggactcag cctacccggt gggcacgacc gacaccgccg aactcttgga caatctcgag
     2281 gtgcccttgg aagcgtcagt gagaacagct caggaagcac tcaaagaggc cggcgaaggg
     2341 cgccggcaac aggtcattcg agcagcgctc aagtggcgcc gcgagcatca ggaatgtgcg
     2401 tcgtgaagct cgggatacac ctcgggatac acctagcagg aatccgccgg gatacacc
//
LOCUS       DASRMJ010000192         5529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_417715, whole genome shotgun sequence.
ACCESSION   DASRMJ010000192 DASRMJ010000000
VERSION     DASRMJ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417715"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFD74_05310"
     CDS             <1..238
                     /locus_tag="VFD74_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZK49001.1"
                     /translation="QVDGENTEDMMFTIENVACLGACGMAPVMMINDRTYGKLTADKA
                     VNALSDFIATQEGGDGGDPVTSGGSNASDEEEEG"
     gene            239..2125
                     /locus_tag="VFD74_05315"
     CDS             239..2125
                     /locus_tag="VFD74_05315"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZK49002.1"
                     /translation="MSVVASLKKPAPQHERRGQPTVEVGICLGTGGIAAGGVDVFAAF
                     EDGLAATGTYAVVKPREDSCEGSCSSVTGTGCRGMCAMDVLVDVVTHRDGVESKVTYG
                     TVTPKMVQKIIEDHIVGGEPVHKWIVLSEDEPTSHNEFYQHQDRLVLRNCGAIDPEAI
                     DDYLERGGYQGLYQVLTGMTPTQVHEQILASGLRGRGGAGFPTGLKWKFAAEAPTPDG
                     QKYFICNGDEGDPGAFMDRSVLEGDPHSVFEGMAIGGYAIGASEGYVYVRAEYPLAIK
                     RLKIALAQAEERGFIGDNIMGSDFSFRLKIKQGAGAFVCGEETALMQSIEGKRGMPRL
                     RPPFPAQSGLWARPTNINNVETLACVPWIIANGAAAFSQRGYEKSKGTKVFALTGKVK
                     RSGLAEVPMGLTLRDVIYSIGGGILNDKPFKAVQMGGPSGGCLPEALLDTKVDYEALN
                     QTGAIVGSGGMVVLDADNCIVDTARFFLNFTQAESCGKCVPCRVGTKRMLETLTRICE
                     GKGVPADIPLLERLAEDIKAGSLCALGGTAPNPVLTSLKYFREEFEEHINEGKCRAGA
                     CAALTTYSIDPETCTGCRACVRACPVDAIAGEKKEVHIIDSAVCIRCGACYDKCRFDA
                     VRRQ"
     gene            2126..4879
                     /gene="fdhF"
                     /locus_tag="VFD74_05320"
     CDS             2126..4879
                     /gene="fdhF"
                     /locus_tag="VFD74_05320"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3209..3211,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZK49003.1"
                     /translation="MAIVREAGEKTLTLTMNGQEVAAAPGQTILEVARSAGIDIPTLC
                     HDDRLEPYGACRMCLVEVEGARGPVPACATKVGDGMVVQTHTDKIRRLRKFVIELLLS
                     NHPLDCPVCEAAGDCRLQDYAYEYEVSMSPWGWHPLEFETRDDHPNVARNPNRCILCG
                     RCVRICREVMGIGCWGFTNRGYDTIIDTPYNLPLEEVGCVSCGQCVSTCPVGALSHKR
                     SRYAARHWQTEKTRTICPYCGVGCEIVVHTNKQNLVRITADVGSGVNNGNLCVKGRFG
                     MDFQNSPDRLTQPLVRDENGDLVAATWDVALERVTQNFERIHAESGGKAFAVLASAKC
                     TNEENYVLQKFARGVLGTNNLDHCARLUHSSTVAGLATAFGSGAMTNSIDDLHEADVI
                     FIIGSNTSEAHPVLSLQVIRALREGKTLIVADPRRIDLARKAHLHLPLKPGTNVALLK
                     GMMRHIVDMGWEDAAFVAQRTENFDQFRESLQSMTVEQAAEICGVPADDIRKAAEMYA
                     KAGAGTILYAMGITQHHTGTNNVLSVANLAMLTGNLGRPGTGVNPLRGQSNVQGACDM
                     GGLPDVFSGYQRVDNPDLVAKMEAAWGVTLADYEKGKTVVEMMNSVGADGIRAMYVMG
                     ENPFLSDPDQHHVEEALKALEFLVVQDIFLTETAQFADVVLPAASNFEKDGTFTNTER
                     RIQRVRKALEPKGESRSDWEIVQSIANRLGAEWNYTSAESIMQEVTEVTPQYGGVTYA
                     RLEGLVPDVEYAQTDVCGLQWPCPTEDHPGTPILHSQQFARGKGLFSAIEYIEPDEQV
                     DAEFPFVLTTGRELVHYHTGTMTRQSAGLNALVPEGRLELNPADAARLGIEDGAKVRV
                     SSRRGSIEPVAWVTDRVSPGLVFMPFHYVEAPANRLTNPVLDPIAKIPEFKVASVKVE
                     RVV"
     gene            complement(5016..>5529)
                     /locus_tag="VFD74_05325"
     CDS             complement(5016..>5529)
                     /locus_tag="VFD74_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK49004.1"
                     /translation="TVDVVIMDITMPDMDGIETTAFITRHHPEVKVLVLSAHEDRESL
                     FAAIEAGAAAYLLKDTEPAELSRAIAVVAQGGSIVSPAVTPHLLQGIREMGYDPARAE
                     RERFNLSDREIEILTHLATPRSPAQIGKALFISTKTVQNHIANIYRKLGVNSRMEAVT
                     KAAGLGVITR"
BASE COUNT         1030 a         1704 c         1862 g          933 t
ORIGIN      
        1 acaggtcgat ggggagaaca ccgaggatat gatgttcacc atcgagaacg ttgcctgcct
       61 gggcgcatgc ggcatggccc cggtcatgat gatcaacgat cgcacctacg gaaagctcac
      121 ggccgacaag gccgtcaacg cactctcaga cttcatcgcc acgcaggaag gcggcgatgg
      181 gggagacccg gtcacatcag gcggttctaa cgcgtccgac gaggaggagg agggctagat
      241 gtcagtcgta gcttcgctga agaagcccgc tcctcagcat gagcgccgcg ggcagcctac
      301 cgtcgaggtg ggcatatgcc tgggcaccgg cggcatcgcg gccggcggcg tggacgtctt
      361 cgccgcgttc gaagacgggt tggctgcgac aggtacctat gcggtggtga agccccggga
      421 ggattcgtgc gaggggtcct gctcgtcggt caccggcacc ggctgcaggg gcatgtgcgc
      481 catggacgtg ctggtcgatg tggtgacgca ccgcgatggc gtggagagca aggtcacata
      541 cggcaccgtc acgccgaaga tggtccagaa gatcatcgaa gatcacatcg tgggcgggga
      601 accggtgcac aagtggatcg tcctctcgga ggacgaaccc acgtcgcaca acgagttcta
      661 ccaacaccaa gaccggctcg tcttgcgcaa ctgcggcgcc atcgacccag aagctataga
      721 cgactacctc gagagaggtg gctaccaggg gttgtatcag gtgctcaccg ggatgacacc
      781 cacccaggtg cacgagcaga tactggcttc ggggctcagg gggaggggcg gcgccggctt
      841 ccccaccggc ctgaaatgga aattcgccgc ggaggcgccc actccggacg gtcagaagta
      901 cttcatctgc aacggcgacg agggcgaccc gggcgcgttc atggaccgtt ccgtcctgga
      961 aggcgatccg cactccgtct tcgagggcat ggccatcggc ggctacgcca tcggcgcgtc
     1021 tgagggctac gtgtatgttc gggccgaata ccccctggcc atcaagcgcc tcaagatcgc
     1081 tctggctcaa gcggaagagc ggggcttcat cggcgacaac atcatgggca gcgacttctc
     1141 ctttcgtctg aagatcaagc agggcgccgg ggctttcgtc tgcggcgaag agacggctct
     1201 catgcagtcg atcgagggca agaggggcat gccacggctg cggccgccct tcccggccca
     1261 gtccggtctg tgggcccggc ccacgaacat caacaacgtg gagacgttgg cctgcgttcc
     1321 gtggatcatc gccaacggag cggcggcctt ctcgcagcgt ggttacgaga agtccaaagg
     1381 caccaaggtc ttcgcgctca ccggaaaggt caagcgctcg ggtctcgccg aagtgcccat
     1441 ggggctcacc ttgcgcgatg tgatctactc catcggcggg ggcatcctga acgacaaacc
     1501 cttcaaggcg gttcagatgg gtggtccgtc cggcggctgc ctgccggagg cgctgctcga
     1561 tacgaaggtg gactacgaag ccctcaacca gaccggggcc atcgtgggct ctgggggcat
     1621 ggtggtgctc gatgccgaca actgcatcgt cgacaccgct cggttcttct tgaacttcac
     1681 gcaggctgag agttgcggca agtgcgttcc ctgccgtgtg ggcaccaagc gcatgctcga
     1741 gacgctcacc cgtatctgtg aagggaaggg cgtccccgcc gacatcccgc tgctcgagag
     1801 gttggccgaa gacatcaagg cgggatcgtt gtgtgctctt ggcggcaccg cgccgaaccc
     1861 cgtgctgacc tcgctcaagt actttcgcga ggagttcgag gaacacatca acgagggcaa
     1921 gtgccgggcg ggagcctgcg ccgcgctcac gacctactcg attgacccgg agacgtgcac
     1981 cggctgtcgt gcatgcgtgc gggcgtgccc cgtcgacgcc atcgcgggcg agaagaagga
     2041 ggtccacatc atcgactctg ccgtctgcat ccggtgcggg gcctgttacg ataaatgtag
     2101 gttcgacgcg gtgaggaggc agtagatggc tatcgtacgc gaagccgggg agaagaccct
     2161 cacgctgacc atgaatggcc aggaagtggc ggcggctccc ggccagacga ttctggaagt
     2221 tgcgcgctcg gctgggatcg atataccgac gctctgccac gacgaccggc tcgagcccta
     2281 cggagcctgc cgcatgtgct tggtcgaggt cgaaggggca cgcgggcccg tgccggcctg
     2341 cgccaccaag gtgggcgacg gcatggtcgt gcagacccac acagacaaga tccgtcggct
     2401 gcgcaagttc gtgatcgaac tcctgctcag caaccacccg ctcgattgcc ccgtgtgcga
     2461 agcggccggg gattgtcgcc tgcaggacta cgcttacgag tacgaggtga gcatgagtcc
     2521 gtggggctgg catcccctgg agttcgagac tcgtgatgac caccccaacg tggcccgcaa
     2581 ccccaaccgc tgcatcttgt gcggccgctg cgtgcgcatc tgccgcgagg tcatgggcat
     2641 cgggtgctgg ggcttcacca accgaggcta cgacaccatc atcgatacgc cctataacct
     2701 tcccctggaa gaggtgggat gtgtctcctg cggccagtgc gtcagcacgt gcccggtggg
     2761 ggcgctcagc cacaagcgga gccggtacgc ggcgcgccat tggcagaccg agaagacccg
     2821 caccatctgc ccctactgcg gggtcggctg cgagatcgtg gtccacacca acaagcagaa
     2881 cctggtgcgg atcaccgcgg acgtggggag cggcgtcaac aacggcaacc tctgcgtcaa
     2941 aggccggttc gggatggact tccagaactc ccccgaccgc ctcacgcaac cgctggtgcg
     3001 cgacgagaac ggcgatctgg tcgccgccac ctgggacgtg gccctggagc gcgtgactca
     3061 gaacttcgag cgcatccatg cggagagcgg cggcaaggca ttcgcggtgc tcgcgtcggc
     3121 caagtgcacg aacgaggaga actacgttct tcagaagttc gcgcggggtg tcctcgggac
     3181 gaacaacctg gaccactgcg cccgcttatg acacagctcc acggtcgccg gtctggcgac
     3241 ggcttttgga agtggagcta tgaccaactc catcgacgat ctgcacgaag cggatgtgat
     3301 cttcattatc ggttcgaaca cctccgaggc tcacccggtg ttgtcgttgc aggtgatcag
     3361 ggccctccgc gaagggaaga ccttgatcgt ggccgatcct cggcgcatcg atctggcccg
     3421 caaggcccac ctgcatctac cgttgaaacc gggcacgaac gtggccctgc tcaaaggcat
     3481 gatgcgccac atcgtggaca tgggatggga agacgcagcc ttcgtcgcgc agcgcaccga
     3541 gaacttcgac cagttccggg aatcgctcca gagcatgacg gtggagcagg ctgcggagat
     3601 ctgcggagtg cctgcggatg acatccgcaa agcggccgag atgtacgcga aggccggggc
     3661 cggcaccatc ctgtacgcga tggggatcac gcagcaccac accggcacca acaacgtgct
     3721 ctccgtagcc aatctggcga tgctgacggg caacctcggt aggcccggca cgggagtcaa
     3781 ccccctgcgc ggccagtcga acgtgcaggg cgcctgcgat atgggcggtt tgcccgacgt
     3841 cttctctggc taccagaggg tggacaatcc agatcttgtc gcgaagatgg aggcggcctg
     3901 gggcgtcacg ctcgccgact acgaaaaggg caagacggtg gtggagatga tgaattccgt
     3961 cggtgccgac ggcatccgcg ccatgtacgt gatgggtgag aacccgttcc tctccgatcc
     4021 cgaccagcac catgtcgagg aagcgctcaa ggcactggag ttccttgtgg tgcaggacat
     4081 cttcctcacg gagaccgcgc aattcgccga cgtcgtcctg cccgcggcca gcaacttcga
     4141 gaaggacggc acgttcacga acaccgagcg ccgcatccag agggtgcgca aggcgttgga
     4201 gcccaaaggc gagagccgtt cggactggga gatcgtccag tcgattgcca atcggctggg
     4261 ggctgagtgg aactacacgt cggccgagag catcatgcaa gaggtcacgg aggtcactcc
     4321 acagtacgga ggcgtcacct acgcccgtct tgagggtctg gtccccgacg tcgagtacgc
     4381 gcagacagac gtgtgcggcc ttcagtggcc gtgcccgaca gaagatcatc cggggacgcc
     4441 gatcctgcat tcgcagcagt tcgcccgcgg gaaagggttg ttctcggcga tcgagtacat
     4501 cgagcccgac gagcaggtgg acgcggagtt ccccttcgtg ctcaccaccg gccgcgagct
     4561 cgtgcactac cacaccggca ccatgacccg ccagtccgcg gggttgaacg cgctggtccc
     4621 ggaggggcgc ttggaactga accccgcgga cgccgctcgt ctcggtatcg aagatggggc
     4681 caaggtgcgt gtgtcgagca ggcgcggctc gatcgagccg gtcgcatggg tcaccgacag
     4741 ggtctcgccg gggctggtgt tcatgccgtt ccactacgtg gaggcgccgg cgaaccggct
     4801 caccaaccct gtgttggatc cgatcgcgaa gataccggag ttcaaggtgg ccagcgtgaa
     4861 ggtagaacgc gtggtctgag agtgcgagtg gcgggctgtg tcggcgtgga cgccgcgccc
     4921 cggctaggcc cgaagaagga acgcgatggc cgcggccccc ccaggggccg cggccatcgc
     4981 gcgtacacag ggggccaagc ccgccgccgc cgaggttacc ttgtgatcac tccgagtccg
     5041 gcggccttcg tgaccgcctc catgcggctg ttcaccccga gcttgcggta gatgttcgcg
     5101 atgtggttct gcaccgtctt cgtggagatg aagagggcct tgccgatctg cgcgggactc
     5161 ctcggagtgg cgaggtgagt gaggatctcg atctcgcggt cggagaggtt gaatcgctct
     5221 cgctcggcgc gggcggggtc gtatcccatc tcgcgtatgc cctgcaggag gtgaggggtc
     5281 accgcagggc ttacgatcga cccaccctgc gcgaccaccg cgatggctcg ggagagctca
     5341 gcgggttccg tgtccttgag gaggtaggcg gcggcgcctg cctctatggc ggcgaagagg
     5401 gattcccggt cttcgtgcgc gctcaacacc aacactttga cctcagggtg gtgccgggtg
     5461 atgaaggcgg tggtctctat gccgtccatg tcgggcatgg ttatgtccat gatgaccacg
     5521 tccacggtg
//
LOCUS       DASRMJ010000193         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_135570, whole genome shotgun sequence.
ACCESSION   DASRMJ010000193 DASRMJ010000000
VERSION     DASRMJ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135570"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VFD74_05330"
     CDS             <1..1563
                     /locus_tag="VFD74_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49005.1"
                     /translation="ERDRVLIDEAVDDAQRRDPGRLTAALEYLRRLLSLRPSLDGPSL
                     DEALELVLAWQQVSGPAMAKGVEDTAFYRYNRLISLQEVGGEVGGDPDAIAEFHRHNL
                     NTLALRPDSMSATSTHDTKRSEDVRARIDVLAEAADAFTAAVTRWHHLNAHAADELDP
                     RSDSAGPRGPSAGPRADLLVPDPNEEHLLYQTLLGAWPLRREELPAFRERVAAFLVKS
                     LREAKEDTSWLQPNLAYEDALQAWVGAILTPDGPSGAGNVPPEPGGGPPRPTSVVSDP
                     TSVVFGRGGAFLEDFLPLQRQVAWYGMLVSLAQVVAKVAAPGIPDFYQGSYLWDLSLV
                     DPDNRRPVDFGERHRILLDLDEAADPTPAPRTAELLRNWTDGRIKLFVTRRALHARRA
                     ASALFARGAYLPLQPAGHHAERVLAFARHHEESWAVVVVPRLTVGLAAAQAAPLGPSV
                     WGNTRVPLPPGAPAVWTEVCSGTLVHAVDEAGNAARRKPADRGAFNQGSASVFVGDAL
                     RYLPVALLRADE"
     gene            complement(1593..1910)
                     /locus_tag="VFD74_05335"
     CDS             complement(1593..1910)
                     /locus_tag="VFD74_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK49006.1"
                     /translation="MRKAKKERLESAGWRVGDAAEFLELTSEEAAFVELKLALAQYVR
                     ELRGAHGWTQAQVARRLGSSQSRVAKMEAADPSVSLDLLVKALLALGASRRDVGWVIA
                     RAA"
     gene            complement(1916..2254)
                     /locus_tag="VFD74_05340"
     CDS             complement(1916..2254)
                     /locus_tag="VFD74_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZK49007.1"
                     /translation="MGKPLVWLRGEVRTPPFSASARVEAGVLLRGLQRGDAVGPPHSR
                     PMPSIGDRCHELRITDGDVAWRLVYRVDTDAVVIVDVFKKKSRATPRRVIDDCRRRLR
                     AYDVIAAARE"
     gene            complement(2493..>2759)
                     /locus_tag="VFD74_05345"
     CDS             complement(2493..>2759)
                     /locus_tag="VFD74_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZK49008.1"
                     /translation="GKLLNPNFRDYKMLTALDSVDVEPILIEDPAAAGPYGAKGIGEP
                     GLVPTAPAIANAIYDAVGIRLHKLPMKPEMVLAALMEKDRRAAG"
BASE COUNT          451 a         1003 c          868 g          437 t
ORIGIN      
        1 gagcgcgacc gcgttctcat agacgaagcc gtggacgacg cgcagaggcg cgaccccggg
       61 cgcctgacgg cggccctcga gtatctgcgc cgtctactca gccttcgccc gtcgcttgat
      121 ggaccgtctc ttgacgaagc cttggagctg gtactcgcct ggcagcaggt gagcgggccg
      181 gctatggcca aaggtgttga agacacagca ttctatcgat acaaccgcct gatctccctg
      241 caggaagtgg gtggagaggt gggcggcgac ccggatgcca tagccgagtt ccaccggcac
      301 aacctgaaca cactggcgct ccggcccgac agcatgagcg ccacgagcac ccacgacacc
      361 aagcgcagtg aagacgtgcg ggcgcgcatc gacgtcctgg ccgaggcggc cgacgccttc
      421 accgcggccg tcacccgctg gcaccatctg aacgcccacg ccgccgacga actcgacccc
      481 cgctcagaca gcgcgggccc ccgcggccca tccgccgggc cccgcgctga cctcctcgtc
      541 cccgatccga acgaggagca ccttctctac cagaccctgc tgggcgcctg gccgctgcga
      601 cgagaggaac tcccagcctt ccgcgagcgc gtggccgcat tcctggtcaa gtccctgcgg
      661 gaggcgaagg aagacaccag ctggctgcag cccaaccttg cctacgagga tgccctgcag
      721 gcgtgggtgg gggccatcct cacgcccgac ggtccgagcg gcgccggcaa tgtcccgccc
      781 gagcccggcg gcggtccgcc ccgcccaacc tccgtcgtgt ccgacccaac ctccgtcgtg
      841 ttcggccgag gcggcgcctt ccttgaggac ttcctcccgc ttcagagaca ggtggcctgg
      901 tatggcatgc tggtctccct cgcccaggtg gtagccaagg tggcggctcc cgggataccg
      961 gacttctatc aggggtccta tctatgggat ctgagcctgg tcgaccccga caatcgccgg
     1021 ccggtggact tcggcgagcg acaccggata ctcctcgatc ttgacgaagc ggccgatccg
     1081 acgcccgccc cccgcacagc ggaacttctg aggaactgga ccgacggacg catcaaactg
     1141 ttcgtcaccc gccgcgccct acatgccagg cgtgcggcct cggcgctctt cgcccgcggg
     1201 gcctacctac ccctccaacc tgcggggcac cacgcggaac gggtcctcgc cttcgcccgc
     1261 caccatgagg agagctgggc cgtggtcgtc gtgccacgac tgacggtggg cctggccgcc
     1321 gcgcaggcgg cgcccctggg gccgtcggtg tggggcaaca cacgcgtgcc tctgcctccg
     1381 ggggcccccg ccgtgtggac cgaggtctgc tccggtacgt tggtccacgc ggtcgacgaa
     1441 gccgggaacg ccgcccggag aaagcccgcc gaccgaggcg ctttcaacca ggggagcgcc
     1501 tcggtgttcg tgggcgatgc cctccgatat ctgcccgtcg ccctgcttcg agccgacgag
     1561 tgaacgtcgc gatcatcggc cgatgagacg actcaagccg cccgagcgat cacccaaccg
     1621 acatcccgac gcgaagcgcc cagagccagc agagccttga cgagcaggtc gagcgagacc
     1681 gaggggtcgg ctgcctccat cttcgcgacc cgagactggc tcgatcccag ccgacgcgct
     1741 acctgcgcct gggtccagcc gtgcgcgccg cggagttccc gcacgtattg cgccaacgca
     1801 agcttgagtt caacaaaggc cgcttcctcc gaggtgagct cgaggaactc agcggcgtcg
     1861 ccgacccgcc atcccgcgga ctcgagacgc tccttcttgg ccttcctcac ggcattcact
     1921 ccctcgcggc ggctatgacg tcgtatgcac ggagccgccg cctgcagtcg tcgatcaccc
     1981 gcctcggagt ggcccgcgat ttcttcttga acacgtcgac aatgacgacg gcatcggtgt
     2041 ctacccgata gaccagtcgc cacgcgacgt ctccgtcggt gatcctcagt tcatgacacc
     2101 gatcgccgat ggacggcatc gggcgcgaat ggggcggacc cactgcatcg ccgcgctgca
     2161 acccccggag caagaccccg gcttccaccc gcgccgacgc agagaacggt ggagttcgca
     2221 cctcgccccg caaccacacg agtggtttgc ccacgcccct ctcctgctgc agtatatgtc
     2281 acttctgaca tggctttcaa gccctcggac agcctccggt cgatagtgac acgcagcctt
     2341 tcttcgagga atacgtagaa ggacgcaggg ataccatgac ggcgcgcacc tttcgctcgc
     2401 tgcgaccgtc atggctgagc gctactcgcg gatcgcagaa gctgcttgtc cgggagagtg
     2461 gccgggaccc cgaggtggta cacgcacgtg ccctaccccg ccgcgcgcct atccttctcc
     2521 atcagcgccg ccagcaccat ctcaggtttc atgggcagct tgtgcaggcg gatgcccacc
     2581 gcgtcgtaga tggcgttggc gatggccgga gccgtcggca ccaggccggg ctcgccgatg
     2641 cccttggctc cgtaggggcc ggcggcggcc gggtcctcga tcaggatggg ctccacgtcc
     2701 acgctgtcca gggcggtcag catcttgtag tcgcggaagt tggggttgag gagtttgcc
//
LOCUS       DASRMJ010000194         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_159648, whole genome shotgun sequence.
ACCESSION   DASRMJ010000194 DASRMJ010000000
VERSION     DASRMJ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159648"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFD74_05350"
     CDS             complement(<1..386)
                     /locus_tag="VFD74_05350"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HZK49009.1"
                     /translation="MIEKRIWVEIGAGDRVSAVVNRPERCVRGETPGLLLSHGAKNDL
                     DHPLLAAVAHGLAALGAATVLRFNFAYAEHGADTPDRLDRLEHVYRRAHDALVDDELC
                     PPGPLFLGGKSLGARVAAGLGARHHEG"
     gene            complement(465..863)
                     /locus_tag="VFD74_05355"
     CDS             complement(465..863)
                     /locus_tag="VFD74_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49010.1"
                     /translation="MKEQRPPAPPISPRFRMVLWLAIGIVPVIIYPILAYMVSNTGYT
                     GQTEYSDSIIGAFVALGIIVLFFNLGLMRRTQAETNDRSYILCLAMAEAIGIEGFVLY
                     LIIGWNWGFWAMLVMSLLSALPLRPRGARG"
     gene            1067..1297
                     /locus_tag="VFD74_05360"
     CDS             1067..1297
                     /locus_tag="VFD74_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49011.1"
                     /translation="MERIIGNLEKVKLKLDEAFFYLDEIEELLQEDLMDAEAGARVGQ
                     AGERLASELLALSGKVVELQDILQALDEREAL"
     gene            1337..1915
                     /locus_tag="VFD74_05365"
     CDS             1337..1915
                     /locus_tag="VFD74_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HZK49012.1"
                     /translation="MSAEWISDGVVHRFDGRPDGCTAGRTAEPTADAERRGERPRLLV
                     SACLVGLCTRYDGGHNRHAAVSGLGGRFTLVPVCPEQLGGLPTPRPPAEIQGEPTEGG
                     AAEVVLACDGRDVSSQLHRGAAETLAVARLVGARAAVLKARSPSCGVHETYDGTFTGT
                     LRPGSGVTAGLLRRAGLDLFTEEDIVAGRMPG"
     gene            1955..2974
                     /locus_tag="VFD74_05370"
     CDS             1955..2974
                     /locus_tag="VFD74_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49013.1"
                     /translation="MARGKAVALLSGGLDSRVAIKMMLDQDIEIEAINFVTPFCTCTA
                     QGCRSEAREASRQFGVPLKVVNNSEALLEAIKHPRHGFGRGLNPCLDCRIISFRKAAE
                     YMETIGATFLVSGEVVGQRPMSQRRKAMDIIEAESGLVGKILRPLSARHFPPTEAEEA
                     GIVDREKLLSISGRGRKEQIALAEEMGINDYPCPAGGCLLTDPGFASRVRELLCEFPD
                     PSMPQVTLLKAGRHFRSSAGEWVIVPREDLENRRLFNLRHVLTAVLEGVDVGGPVAGV
                     LSATASEQTLVEAAALVARYGQARERETVTVRAYAPDESWERFFEASTADGARLAENL
                     DRVGE"
     gene            complement(3110..3892)
                     /locus_tag="VFD74_05375"
     CDS             complement(3110..3892)
                     /locus_tag="VFD74_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZK49014.1"
                     /translation="MSLVRVLVAKEARELLKTLRVIILPAVFVFFGIAGPGIIRLLPI
                     LLKSSEAQGLDMALPDFGPADGFGQFLELARQMGLLAVLIVYMGIVAGERRDGMLATL
                     FVKPVPRSLYLATRWLVNGAYVVVSFLLGAAVALVYTLLLLGRVDVGAAISATLLYAG
                     YMLLAFSWTTFFSAWMKSPPAAAGLSVLPLFALPALGVLWEPLGTYGPYGAVAAGTAA
                     LGMLGLPAVAIPASAVISAVLNLGWSVLLVVGAHAVLRHAEL"
     gene            complement(3889..>4096)
                     /locus_tag="VFD74_05380"
     CDS             complement(3889..>4096)
                     /locus_tag="VFD74_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49015.1"
                     /translation="PWLVECVEEGAALRLLVDDHIAAQVRVPTILSDLSAHLAAFKMD
                     SPSLEDVFIRLVTDGAHAAQENAR"
BASE COUNT          715 a         1309 c         1454 g          618 t
ORIGIN      
        1 ccctcatggt gacgcgcgcc caggccggca gccacccgcg ctccaagcga cttgccgccg
       61 aggaagagcg ggccgggtgg gcacagctca tcgtcgacca gagcgtcgtg agcccgtcga
      121 tagacatgct cgagtctgtc caggcggtcg ggtgtgtccg ctccgtgttc cgcgtaggcg
      181 aagttgaatc ggagtacggt agccgcccca agggccgcca aaccgtgggc taccgctgcc
      241 aggagagggt ggtcgaggtc gttcttcgcc ccgtgggaga gcaacaggcc gggagtctcc
      301 ccgcgcacgc accgttcggg ccggttcacg actgcggaga ccctgtctcc ggccccgatc
      361 tcgacccaga tcctcttctc gatcatgccg tcttctcccg ggtcgaggca cggccgggct
      421 gcgcttccgg cagccggcgg cgcgcgacct tcatcgagtg agccctaccc gcgggctccc
      481 ctcggcctca acggtagggc tgaaagcagc gacatgacga gcatggccca gaatccccag
      541 ttccatccga tgatcagata gaggacgaag ccctcgatgc cgatcgcctc ggccatggcg
      601 aggcaaagga tgtacgagcg gtcgttcgtc tcggcctgcg tgcgtcgcat cagtcctagg
      661 ttgaagaaga ggacgattat cccgagtgcc acgaacgcgc cgatgatgga gtcggagtac
      721 tcggtctggc ccgtgtatcc agtgttggag accatgtagg cgaggatcgg atagatgatc
      781 acgggcacaa tgccgatagc cagccagagc accatgcgaa aacgtggcga tatgggcggt
      841 gctgggggtc tctgctcctt caaggtgttc ctccggtcgg tcgggggctt cggctcggct
      901 ccgtgcggcc acgcggcgag cgggcggacg attcttctcc ctcgtgggat ggtaacctct
      961 tgaggtggaa aagagaaccg cggcgatcag gcgagggcgc gtcggggccg cctcgcagtg
     1021 ttcggcttcg gaacccggcg gcctgtcatc cggaagaagg gaaacgatgg aacggatcat
     1081 cggcaacctg gagaaggtca agctcaagct cgacgaagcg ttcttctacc tcgacgagat
     1141 cgaggaactt ctgcaggagg acctcatgga cgccgaggcg ggagcgcggg tgggacaggc
     1201 cggtgagcgg ctggcctccg agcttctcgc tctgagtggc aaggtggtcg agttgcagga
     1261 catccttcag gcgctcgacg agcgggaagc actgtaggac ggagggctct cgcgagccct
     1321 tgtcggcctg cacgtcgtga gcgcagagtg gatcagcgat ggggtggtgc accggttcga
     1381 cgggcgaccc gacgggtgta ccgccggtcg gactgccgag ccgacggctg acgccgagcg
     1441 ccggggagaa cggccgcgcc tcttggtgag cgcctgcttg gtcggcctct gcaccaggta
     1501 cgacggcggt cacaaccgcc acgcggcggt gtcgggtctt ggcggccggt ttacgctcgt
     1561 tccggtatgc cctgagcagc tcggtggctt gcccacaccg cgacccccgg ccgagatcca
     1621 aggggaaccg actgagggcg gcgccgcgga agtggtgctc gcgtgcgacg gtcgcgacgt
     1681 gtcctcgcag ctccacaggg gcgcggcgga gacgctggca gtcgcccggt tggtgggggc
     1741 gcgggctgct gtgctgaagg cgcggagccc gtcctgcggc gtgcacgaga cctacgacgg
     1801 gacgttcacg ggtacactgc gccccggctc gggcgtgacg gccggcctgc tgcggcgcgc
     1861 gggactcgat ctgttcacgg aagaagacat agtcgccggg cggatgccgg gttgacccga
     1921 cccggtcgtc gagcgatcga ggagagacac gagcatggca cgaggaaaag cggtagccct
     1981 cctttccgga ggcttggaca gcagggtggc catcaagatg atgctcgatc aggacatcga
     2041 aatcgaggcc atcaacttcg tcaccccgtt ctgcacctgc accgcgcagg gttgtcggtc
     2101 cgaggccagg gaggcgtcgc gccagttcgg cgtgcccctg aaggtcgtca acaactccga
     2161 ggctctgctg gaggcgatca agcatccgcg ccacggattc ggccgggggc tcaacccgtg
     2221 tttggactgt cgcatcatca gcttccgcaa ggcggccgag tacatggaga cgataggggc
     2281 caccttcctg gtcagtggtg aggtggtggg gcagcggccg atgtcccaaa gacgcaaggc
     2341 gatggacatc atcgaggccg aatcgggtct ggtagggaag atcctgcggc cgctctcggc
     2401 acgacacttc ccgcccaccg aggcggaaga agcgggcatc gtcgacaggg agaagttgct
     2461 ctcgatcagt ggtcgcggcc gcaaggaaca gatagcgttg gccgaggaga tgggcatcaa
     2521 cgactacccg tgtccggcgg gcggctgcct cctcaccgac ccaggcttcg cgtctcgcgt
     2581 gcgcgagctg ctttgcgaat tccccgaccc gtccatgcct caggtgacgc tgctcaaagc
     2641 ggggcggcac ttccgttcca gcgcaggcga atgggtgatc gttccccggg aggaccttga
     2701 gaatcgccgt ctcttcaatc tgcggcacgt gttgactgcc gttctcgaag gcgtggatgt
     2761 gggtgggccg gtggccggcg tcctctcggc tacggcgagc gagcagacgc tggtcgaggc
     2821 cgccgctctc gtcgcccgct acggacaggc gcgtgagcgg gagacggtga cggtccgcgc
     2881 ctacgcgccc gacgaatcgt gggagcgctt cttcgaggcg tccaccgccg acggcgcgcg
     2941 cctggccgag aacctcgaca gggtagggga gtgaccgtcg caggcgactg actgccgcgg
     3001 cggccgacgg cgccgcgtcc gcgggacggc cgcctgcacg tctgcacaca cgtcagcgcc
     3061 cgcggaccgg ccggggtcgc ttgagcgggg cccggccgag ttacacccgt cagagttcgg
     3121 cgtgcctcag gacggcgtgg gcgcccacga ccaggagcac gctccaccca agattgagca
     3181 ctgcgctgat cacggccgac gcggggatcg ccaccgccgg gaggccgagc atcccgagtg
     3241 cggcagtgcc ggccgccacg gctccgtacg ggccgtacgt tccgagcggc tcccagagga
     3301 cgcccagagc cggaagcgcg aacaggggaa gcacgctgag cccggccgcc gccggcgggc
     3361 tcttcatcca ggctgagaag aacgtggtcc aggagaacgc cagcagcatg tagcccgcgt
     3421 agagaagggt ggcgctgatc gccgcaccca cgtcgacccg cccgaggagc agcaaggtgt
     3481 acaccagcgc cacggccgca cccagcaaga acgagacgac cacgtaggcg ccgttgacga
     3541 gccacctggt cgcgaggtag agggagcgcg gcaccggctt cacgaacagc gtggcgagca
     3601 tgccgtcgcg cctctctccg gcgacgatcc ccatgtacac gatgagcacc gcaagcaggc
     3661 ccatctggcg cgccagctcg aggaattgcc cgaacccgtc ggcaggtccg aagtcgggca
     3721 gggccatgtc gagtccctgc gcctcggaac tcttcaggag gatggggagg agtcggatga
     3781 tcccgggccc ggcgatgccg aagaacacga acaccgccgg caagatgatc acacggaggg
     3841 tcttgagcag ctcccgcgcc tccttagcga cgaggacgcg caccaggctc atcgcgcgtt
     3901 ctcctgggca gcatgggcgc catcggtcac gagccgtatg aagacatcct cgagcgacgg
     3961 ggagtccatc ttgaacgcag ccaggtgcgc cgagaggtcg gaaaggatgg taggcacccg
     4021 gacctgcgcc gctatgtggt cgtccacgag cagccggagg gccgcaccct cctccacgca
     4081 ctcgacgagc cagggc
//
LOCUS       DASRMJ010000195         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_159670, whole genome shotgun sequence.
ACCESSION   DASRMJ010000195 DASRMJ010000000
VERSION     DASRMJ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159670"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFD74_05385"
     CDS             <1..501
                     /locus_tag="VFD74_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZK49016.1"
                     /translation="RAEVFRLTRKDLAFENERWAYTYRGKGGKNGRRELPQPAVEALR
                     AALRARGLDLEVMGLSESLWGITSATFYGQLQRYFREAGLPPAGVHVFRHAAAKLRRE
                     AGESVEEVSLFLDHSSLAVTTTYLRRLEGQEDRGWGRVAEAIGIAAGANSVWPPPESA
                     ARRREP"
     gene            703..>2297
                     /locus_tag="VFD74_05390"
     CDS             703..>2297
                     /locus_tag="VFD74_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZK49017.1"
                     /translation="MKQELSQRQVELEFKSLLLDEATDSIVAHDLVGRVVYANEEAVT
                     RMGYPREEVLGRRLPALLGAPEDAFATRVEQILEKGQAIFEAEDLFADGSFRPVEVHS
                     RVLDSGGQVLICSISRDISDRKHAASAIERLAYYDPLTGLANRRLFNDRLRMAFAHSH
                     RTQDPLSVLFLDLDQLKVVNDSLGHGSGDEVLQTMVKRFQGVVREADTLARLGGDEFV
                     LLLLDAGDQEAVSVADRVLEMVRAPLLIDGQRVDITVSVGIATSDMGAETADILLQQA
                     DMAMYAAKSSGGNGYRLYGHDMTTAALARFRLRNDFRDAFEHKDFRLHYQPLVRLSDG
                     AVSGAEALLRWRHPTLGEVSPAIFIPQAEEIGLIIPLGAWVLSQACRQAQVWREAGLP
                     LERMAVNVSGRQLLHGDLVADVRAALTESDLPPSCLELEITESVALEGSPEVLEVLIA
                     LRSLGVSLAIDDFGAGHSSLSRLRDLPVQTLKIDRKFIAGLSPGTVQDHAIVRTIVEL
                     SRRMGMSTVAEGVETEQEAQLLK"
BASE COUNT          403 a          751 c          755 g          388 t
ORIGIN      
        1 cgggcggagg tgtttcgcct gacccgcaag gatctcgcct tcgagaatga gcgctgggcc
       61 tacacctaca gaggcaaggg cgggaagaac ggacgccggg agctgccgca gcccgccgtg
      121 gaggcgctcc gagcggcgct gcgggcccgg ggcttggacc ttgaggtcat gggtctctcc
      181 gagtctctgt ggggcatcac cagtgccacc ttctacgggc agctgcaacg gtacttccgg
      241 gaggccgggc ttccgccggc cggggtccat gtctttcgac acgcggcggc gaagttgaga
      301 cgagaggccg gggagtcggt ggaggaggtg agcctgtttc tggaccacag ctcgctggcg
      361 gtgaccacta cctacctgcg gcgcctcgag ggccaggagg atcgcgggtg gggacgggtg
      421 gcggaggcga tcgggatagc ggccggtgca aactcggtct ggccgccgcc cgaatctgct
      481 gcccgccgcc gagagccgta aagggcagct ctccccgggc cgatatgacc ctgtgaggca
      541 aaccgtcagg aggaatacgt gcacgcagag aagaccacga gtgcaaagct gccagaatct
      601 cgtccccggc caagccctgc caacccgcat gccaacacca gaaccacggc ctcctcatcg
      661 ccgtcctgct ctcccgactc cccggacagt gaactcgatc aggtgaaaca agagctatcc
      721 cagcgacagg tagaactcga gttcaagtcc ctgctcctcg acgaagccac cgactccatc
      781 gtggcccacg atctcgtcgg ccgcgtcgtc tacgcgaatg aggaggcggt cacccgtatg
      841 ggctaccctc gggaggaggt tctcggccgc cgtctgcccg ctctcctggg cgcccccgag
      901 gacgcctttg ccacccgggt agagcagatc ctagagaagg ggcaggcgat cttcgaggcg
      961 gaggatctgt tcgctgatgg tagcttccgt ccggtcgagg tacacagccg ggtgctggac
     1021 agcggcgggc aggtgctcat ctgcagcatc tcacgggaca tcagcgaccg aaagcatgcc
     1081 gcgagcgcca tcgagcgcct tgcctactac gatccgctca ccgggctcgc aaaccggcgg
     1141 ctgttcaatg accgcctgcg catggccttc gcccattccc accgtactca ggaccccctg
     1201 tcggtcctct ttctcgactt ggaccagttg aaggtggtaa acgattcgct gggacatggg
     1261 agcggtgacg aggtcctcca gaccatggtc aagcggtttc aaggggtggt gcgcgaggcc
     1321 gacacgctgg ctcggctcgg tggcgacgag ttcgtgctgc tcctgctaga cgcgggcgac
     1381 caggaggcgg tatccgtggc cgaccgggtg ctcgaaatgg tgcgggcccc gctcctcatt
     1441 gacggccagc gggtcgacat caccgtttcg gtcggcatcg ccacctctga catgggagcg
     1501 gaaacggccg acattctcct ccaacaagcc gacatggcca tgtacgccgc caagagcagc
     1561 gggggcaatg gctaccgact gtacgggcac gacatgacca cggccgcgct ggcccgtttc
     1621 cgcctgcgga acgatttcag agacgccttc gaacacaagg atttccgtct ccactatcag
     1681 ccgctggtgc gcctgagcga cggcgcagta tcgggagcgg aggctttgct tcgctggcgg
     1741 cacccaacac tgggcgaagt ctcccctgcg atcttcatcc cccaagccga ggaaatcggt
     1801 cttatcattc ccctgggcgc ctgggtactt tcgcaggcgt gccggcaggc tcaggtttgg
     1861 cgggaggcgg gcctcccgct cgagcgtatg gcggtgaacg tctccggacg gcagctcctt
     1921 cacggggacc tcgtcgcgga tgtgcgggcg gccctgaccg aaagcgacct tcccccgagc
     1981 tgcctggaac tcgagatcac cgagagcgtg gctttggagg gttcccctga ggtcctggag
     2041 gtcctcatcg ccttgcgcag cctcggcgtg agcctcgcta tcgacgattt cggtgccggc
     2101 cactcctccc tcagccggct acgggacctt cccgtacaga cgctcaaaat cgaccgcaag
     2161 ttcatcgccg gcctcagccc ggggacggtc caagaccatg cgatcgtgcg caccatcgtc
     2221 gagttgagcc gccgtatggg catgtcaacc gtcgccgaag gggtggagac cgagcaggag
     2281 gcgcagctcc tcaagga
//
LOCUS       DASRMJ010000196         4246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_377075, whole genome shotgun sequence.
ACCESSION   DASRMJ010000196 DASRMJ010000000
VERSION     DASRMJ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377075"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VFD74_05395"
     CDS             <1..1545
                     /locus_tag="VFD74_05395"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZK49018.1"
                     /translation="GGAGSTTEVVPPGSLANLLFRDGGVHIVTVAGGGRGPYAKVEVR
                     PGTGGRVKVQFRISSGDAIRVLDAREYWGKIQVEPSTTSGNLIAYNFVPIEKYLRSIA
                     EVIPNWAQSTSSAYAPEAVKAQAVAARTYAVANKDPYLNDNQWDQVYRGYTYEALYPG
                     IGEAAEQTLDSQGRGLILRYNGKVVVSFFSSSSGGYTSPWYPAATYPYLPAKPDPYSL
                     AAPTSNPGYAWTFSISPADLSAAVDGMSDTGGNAVHIGMLDRVEVASRDTSDPGSHVA
                     TFRLIGVNGSAVVSASSLRGRLGYGQMRSTLVVRITNPRVFSDVPSGHFFLTEIERIA
                     LAGIVQGFPDGKFSPDASVARWQFAKMVVGLHNVLLADDQIAVVDVGTSPFADVPAQP
                     GVLGDESDWVAAAKNAGLVKGFTETVFMPYASVQRDQMATMLVRAMRWEDEAAALPSD
                     TPGFADVAPGSTHWADTRYLKSLGVLRGYEEPPGSGTFVLRWADPTLRMHAAAVLCRV
                     LDLPAG"
     gene            1756..2880
                     /locus_tag="VFD74_05400"
     CDS             1756..2880
                     /locus_tag="VFD74_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK49019.1"
                     /translation="MMGRAGATESAKNRIEGRHLNLAIVAMMLCVLLALLLPVAAQAG
                     TYTAEEMQFVKILNEYRAANGLGTLLVSDMVSVAATKHDMDMAKYSFFDHVTQGSDYY
                     AVGASPWARMAAAGYNYNTTKGENLAAGFSTAAGVFEGWRNSPGHNANMLNGGFKVVG
                     ISMDLVPGSQYLYYWTTDFGGYVDSTSHSVDSLSTQRFIDVPPTHSYASQIEALADLG
                     VISGFGDGYFQPNASVVREQFAKMIVLTLGLPVSESDVPPFADVQVTPGSLYPAAYIA
                     VAARAGITRGVTADRFAPWNKITRAEVITMVVRAANLPEPPADYQPPFGDFSPTFYPY
                     ARKAAYAGILDGLVGMGSSYNFWTPATRGEVCALLSSLMQ"
     gene            complement(2991..3953)
                     /locus_tag="VFD74_05405"
     CDS             complement(2991..3953)
                     /locus_tag="VFD74_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49020.1"
                     /translation="MNAHLVTCGSLLTLTLSLFGLPAPAEAAAPPMSRPEIIARAESA
                     IGTKYTWGRESWSPNTSGPGPDCSGFVLKSWEVPRTMLYQEEDGVNASITPRYTTYSF
                     LNNLGPWTSLSSRADLLPGDALVYNDGTGGHIVLYADGDAWNYPIVYEAPYTGARVRR
                     ASRYVGSAYQPRRRDGLTDSSILIDNPTAASVGGTDVGGNWKRSTSNPGFYGPDYQVQ
                     AATSGTAWARWTPRLPVSGYYNVYMRWTDDWNRASNARVVVATPSSTSTRYLDQRTGG
                     GAWVKIGTYYLGAGYHPTTGSVTIHAGGANGYVVADSVLFALAW"
     gene            complement(3950..>4246)
                     /locus_tag="VFD74_05410"
     CDS             complement(3950..>4246)
                     /locus_tag="VFD74_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49021.1"
                     /translation="TGAAAGSGVGSGAEVAAPGGVGSDAEVADGSEPSLLLVARIGEP
                     VQTISLVDDSAVYLGVPYVVGLDSAVYQPVATDEGYRLVRHTFPETPGFSEGER"
BASE COUNT          703 a         1446 c         1356 g          741 t
ORIGIN      
        1 ggcggagccg gctccacgac cgaagtggtg ccccccggca gtctcgccaa cctgctcttc
       61 cgggacggag gggtccatat cgtgaccgtt gccggcgggg gccggggtcc gtatgcgaag
      121 gtcgaagtcc gcccgggcac cggcgggagg gtcaaggtgc agttcaggat ctcctcgggg
      181 gatgcgatca gggtcctgga cgcccgcgag tactggggca agatacaagt cgagccctcc
      241 accacgtccg gcaatctcat cgcctacaac ttcgtgccca tcgagaagta cctgcgcagc
      301 atagccgagg tcataccgaa ctgggcgcag tccacgtcga gcgcctatgc cccggaggcg
      361 gtcaaagccc aagcggtggc cgcgcgcacg tacgcggtcg ccaacaagga cccatacctc
      421 aacgacaacc agtgggacca ggtctatcgg ggctacacct atgaggccct gtatccgggc
      481 atcggcgagg ctgccgagca gacgcttgac tcccaaggcc gcggactgat acttcggtac
      541 aacggcaagg tcgtcgtcag cttcttctct tcgagttccg gcgggtatac gtccccctgg
      601 tatccggcgg ccacctaccc ctacctgccg gccaagccgg atccctacag cctcgcggcg
      661 cccaccagca atccgggata cgcgtggacg ttctccatct cgcccgccga cctctctgcg
      721 gccgtggacg gaatgagcga cacgggggga aatgccgtac atatagggat gctcgaccga
      781 gtcgaggtcg cttcacgtga caccagcgac cccggcagcc atgtggccac cttcaggctc
      841 atcggcgtca acgggtccgc ggtggtgagc gcctcgtcct tgcgggggcg tctcggatac
      901 ggtcagatgc gctccaccct cgttgtccgg atcacgaacc cgcgcgtctt cagcgacgtg
      961 ccctccggtc acttcttctt gaccgagatc gagcgcatcg ccctggccgg catcgtgcag
     1021 ggcttcccgg acggcaagtt ctcccccgac gcttccgtgg cccgttggca gttcgccaag
     1081 atggtggtcg gtctgcacaa cgtcctcttg gccgatgacc agatcgccgt ggtcgacgtg
     1141 ggaacctccc cgttcgcgga cgtgccggcg caaccgggcg tactaggcga cgagtccgac
     1201 tgggtggcgg ccgccaagaa cgcggggctg gtgaagggtt tcaccgaaac tgtcttcatg
     1261 ccgtacgcct cagtacagcg cgaccagatg gctacgatgc ttgttcgggc gatgcgctgg
     1321 gaggacgagg cggccgccct accctcggac acccccggtt tcgccgacgt cgcgccgggc
     1381 agcacgcatt gggccgatac ccgctacctg aaatcgctcg gcgtcctccg gggctacgag
     1441 gagccgccgg gttcaggcac cttcgtgctt cgctgggctg atcccacctt gcgcatgcat
     1501 gccgccgccg tactctgccg cgtgttggat ctgcccgcag gctgaagctg ccccccgtca
     1561 tctctttccg cagcgccccg ctgccaccgt ctccttgccc gccgtccgcc gcttccctcc
     1621 cccgcgtcag cggccccatc cagatgcgcc cctgcgggtt ctccgcacac tcatgatccg
     1681 ctaaagattc cgcgtctgta tgccgatatt gccgcgtagg tagaaatgga tgggcatatc
     1741 ttgcggaggg cggtcatgat gggacgagcg ggagcgaccg aaagcgcgaa gaatcggatc
     1801 gagggccgcc acctcaacct ggcgatcgtg gccatgatgc tctgtgtgct gctggccctt
     1861 cttctcccgg tggcggcaca ggccggcacc tacaccgccg aagagatgca gttcgtgaag
     1921 atcctcaacg agtatcgggc tgcgaacggc cttgggacct tgctcgtctc cgacatggtc
     1981 tctgtcgccg ccacgaagca cgacatggac atggcgaagt acagcttctt cgaccatgtc
     2041 acgcaaggct ccgactacta cgcagtggga gcgagcccgt gggcccgcat ggcggccgcc
     2101 ggctacaact acaacaccac caagggtgag aacctggcgg cgggtttctc gactgccgct
     2161 ggagtgttcg aaggctggcg caactccccg ggccacaacg cgaacatgct gaacggaggc
     2221 ttcaaggtcg tcgggatctc gatggatctc gtacccggct cgcagtacct ctactattgg
     2281 accaccgatt tcggcgggta cgtcgactcc acctcgcaca gcgtggatag cctgtccacc
     2341 cagcgcttca tcgatgtgcc gccgacccac tcgtatgcct cgcagatcga ggccctcgcc
     2401 gacctgggcg tcatctccgg attcggcgac ggctacttcc agcctaacgc ctcggtcgtg
     2461 agggagcagt tcgcgaagat gatcgtgttg accctggggc tacccgtaag cgagagtgac
     2521 gtgccgccgt tcgccgatgt gcaggtcaca ccgggcagtc tctacccggc cgcctacatc
     2581 gcggtggcag ccagagcggg gatcacgaga ggcgtcactg ccgaccgctt cgctccgtgg
     2641 aacaagatca cgcgggcgga agtgatcacc atggtagtgc gggcagccaa tctccccgag
     2701 cccccggccg actatcagcc cccgttcggc gacttctctc ccaccttcta cccctacgcg
     2761 cggaaagccg cgtacgccgg catcctcgac ggactggttg gaatgggatc gagctacaat
     2821 ttctggacgc ccgccacacg cggtgaggtg tgcgccctcc tgagcagcct gatgcagtag
     2881 caggaggcag ccgcgagaag aggttctccc ctcgaccggg tgggccggca agagccggcc
     2941 caccctccac cctccggtcc agggtcactc gattccctcg gccacccggt ctaccacgcg
     3001 agcgcgaaca agaccgagtc ggccaccacg tagccgttcg ccccaccggc gtgtatggta
     3061 acgctccccg tggtgggatg gtatccggcg ccaaggtagt atgtgcctat cttgacccag
     3121 gccccgcccc ccgttcgttg gtccagatac cgggtggagg tgctggaggg ggtcgcaacc
     3181 accactctgg cgttcgacgc gcggttccag tcgtcggtcc aacgcatgta gacgttgtag
     3241 tagccggaga ccggcaggcg gggggtccag cgcgcccacg cggtccccga ggtcgctgcc
     3301 tgcacctggt agtccggacc gtagaacccc gggttgctgg tggagcgttt ccagttgccg
     3361 cccacgtctg tgccgccgac gctggctgcg gtggggttgt cgatcagtat gctggagtcg
     3421 gtgagaccgt cgcgccgtcg cggctggtac gcggacccca cgtagcggga ggcccggcgc
     3481 acccgggcac ccgtatacgg tgcttcgtac acaatcgggt agttccatgc gtcgccatcg
     3541 gcgtagagca cgatgtgccc ccccgtgccg tcgttgtaga ccagcgcgtc gccgggaagg
     3601 aggtccgccc ggctgctcag tgaggtccag ggtcccagat tgttcagaaa gctgtaggtg
     3661 gtgtagcgcg gagtgatgct cgcgttcact ccgtcttcct cttggtacag catcgtgcgc
     3721 ggcacttccc agctcttgag cacgaatccg gaacagtcgg ggccggggcc ggaagtgttc
     3781 ggagaccaac tctctcgccc ccaggtgtac ttcgtgccga tggccgactc cgcgcgggcg
     3841 atgatctccg gtcggctcat cggaggtgcc gccgcctcgg cgggcgccgg cagtccgaag
     3901 agggagaggg tcagcgtcag caacgaaccg catgtgacca gatgggcgtt catctctctc
     3961 cctccgagaa tccgggggtt tcagggaagg tgtgtctgac gagccggtag ccctcatcgg
     4021 tggccaccgg ctggtagacg gctgaatcca gccccacgac gtacgggacg ccgaggtaga
     4081 cggcgctgtc gtcgacgagc gagatggtct ggaccggttc gccgatgcgt gccaccagca
     4141 gcaacgaagg ctcggaccca tcggccactt ccgcgtccga gcccacgccc cccggggcgg
     4201 ccacttccgc gcccgagccc acgccggatc ccgcggcggc ccccgt
//
LOCUS       DASRMJ010000197         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_522362, whole genome shotgun sequence.
ACCESSION   DASRMJ010000197 DASRMJ010000000
VERSION     DASRMJ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522362"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VFD74_05415"
     CDS             complement(<1..1104)
                     /locus_tag="VFD74_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49022.1"
                     /translation="MTPDQQSLLEAIVDCVPAWYIADTGPVQHAVRLGEDSFYFYSFS
                     PNSPRHDLLLQAINAEVGEWARDMPSPPTRSYLAAVREKVALHWLRLHEPGVVWNRFL
                     SHLDELAYRTHENRRVTRNLIVRHGQEGFVPVDDPDLQSVLDPLAGSPQTYLAIGYQG
                     RYLEYGEVLYGGIEDTEHYDLHPEFLQPLKSQLGPRDYSLHLTERGDSIILGAEGMLA
                     AHRRGKWYIYDAERLQNDLFSTLGEYLVSAHLFGVVLDLSYGGLGALLVYDPNGTVLE
                     QVINADEAVIGGPEPRGDKARRLLAGDIGPIRMSDPRLAKRKKRLFLEIASLDGAVIF
                     DDRGVLAFGAMIRSNPGVGGHFGARTTAMHSAYL"
     gene            complement(1141..1647)
                     /locus_tag="VFD74_05420"
     CDS             complement(1141..1647)
                     /locus_tag="VFD74_05420"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZK49023.1"
                     /translation="MASDQIEALVTRLGDKDGGVRQRARETLAAIGEPALPALLELLA
                     SPQVRMRWEAAKALTESPDPVAIPGLVALLADDESDLRWLAAIGLVNIGNRSLPYVLQ
                     ALTERAESKGLREASHHVFRDLSDRNGVIRGILEPVLEVLGDTDAIGVIYARAEVALR
                     KLRTLRGD"
BASE COUNT          425 a          812 c          844 g          432 t
ORIGIN      
        1 caggtaggcg gagtgcatcg ccgtggtgcg cgccccgaag tgcccaccca cgccgggatt
       61 ggacctgatc atagcgccga acgccagcac gcctcggtca tcgaagatca ccgccccatc
      121 gagggacgcg atctcgagga acagccgctt cttgcgcttc gcgaggcgcg ggtcggacat
      181 ccggatgggg ccgatgtccc cggcgaggag ccgcctcgcc ttgtcgcccc ggggttcggg
      241 cccgccaatg acggcctcgt cggcgttgat cacctgctcg aggaccgtgc cgttgggatc
      301 gtagaccagc agcgccccca ggccaccgta gctcaggtcc agcaccactc cgaacagatg
      361 ggcactcacg aggtactcgc cgagggtgct gaagaggtcg ttctgcaggc gctcagcatc
      421 gtagatgtac cacttccccc ggcgatgcgc cgcaagcatg ccctcggccc ccaagatgat
      481 ggaatctccc cgctcggtga ggtgcagcga gtagtcccgc gggccgagct gcgatttcag
      541 cggctgcagg aactcggggt gcaggtcgta gtgctcggtg tcttcgatgc cgccatagag
      601 cacctcgccg tactccaggt agcgcccctg atagccgatg gccaggtagg tttggggcga
      661 gcctgccagc ggatccagta ccgactgcag gtcgggatcg tcgacgggca cgaatccctc
      721 ttgtccgtgt cgcacgatca ggtttcgcgt cacgcgacga ttctcgtgcg tacggtaagc
      781 cagctcgtcc aggtgggaga ggaaccggtt ccacacgacg ccgggctcgt gcagccgcag
      841 ccagtgcagg gcgaccttct cccggacggc ggccaagtag ctcctcgtcg gcggggaggg
      901 catgtcacgc gcccactctc cgacctccgc attgattgcc tggagcaaca gatcgtgccg
      961 cggcgagttc gggctgaagc tgtagaagta gaagctgtcc tcgccgagcc tgacggcgtg
     1021 ctgcacgggg ccggtgtcgg cgatgtacca cgcgggcacg caatcgacaa tagcctccag
     1081 gaggctctgc tggtcagggg tcatcgggca cgctccgcgg cgcccgcata acgcgccacg
     1141 tcagtccccg cggagcgtgc ggagtttgcg cagggccacc tcggcccgcg cgtagatgac
     1201 gccgatcgcg tcagtgtcgc cgaggacctc gagcacgggc tcaaggatgc cccgaatgac
     1261 accattgcga tccgacaggt cgcggaagac gtggtgggag gcctcccgca gacccttgga
     1321 ctccgcccgc tcggtcaggg cctgcagcac gtacgggagg gagcggttgc ctatattgac
     1381 gagcccgatg gccgccaacc agcggaggtc ggattcgtca tcggcaagca gggcgaccaa
     1441 gccggggatt gccacgggat cggggctctc cgtcagcgcc ttggcggcct cccagcgcat
     1501 gcgaacctgc gggctggcga gcaactctag gagtgcaggt agcgccggtt caccgatggc
     1561 cgccaacgtc tcccgggccc gttggcgcac accgccgtct ttgtcgccta gtcgcgttac
     1621 cagtgcttcg atctgatcgg atgccatctg cacctccgtg tgcacgtgag cgccgggttc
     1681 acgacatgcc ttgcggagcg cggcgttcga accctccgca cgccttcttc ccgcgaacgg
     1741 ccattctcag acaggcgaga tcgggtcggg ggctgtcgtg gccatcggcg tcggtggaat
     1801 ggtcgggggc gggatcttcg ccgtgttggg ccttgcggtg cagttcgcac gcggcggcac
     1861 accagtggcc tttgcattgg cgggcgggct tacgacgccc ccgtacactc atgaccgaag
     1921 ggtcagacac tcgagaaagt agtctccggt cgagattcgc gctggccgag agacaagacc
     1981 aaggatgtgg gtatgaacat actgatccgc aggcgcccgc gctgctccaa caccatgacc
     2041 cacttcacct gtccgcccgg ctcaagcgcc tgggtcttcg cgccccccgg gagggttcag
     2101 gggacgatct ggtggcgaac tcggcagcag gcaagggaac gagatggacg ataggctctg
     2161 gacaccatgg tcgtccgacc cctgcgtcga cccgcgtagc gccagctcga tgcttcgaag
     2221 agccgcccta cccacccttg gtcgcaaggg ttaacggtca gcccggcagc gtgttcgtgc
     2281 cacgcacgca atggctcgca ttgcggaaat ccagcctcgg ctgtgttaga tagtcacgga
     2341 ttctgagcac tacgcgcaga gactcgacag gcgacacgat cttaggtagg gggttcgacc
     2401 atgagcattc tggcgtggat cgtcttcggt ggcctagcgg gcttggtagc cagcttcctc
     2461 atgggcggcg gcgggggcat gatcttcgac atcatcgtgg gcatagtggg ggc
//
LOCUS       DASRMJ010000198         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_159897, whole genome shotgun sequence.
ACCESSION   DASRMJ010000198 DASRMJ010000000
VERSION     DASRMJ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159897"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VFD74_05425"
     CDS             <1..1052
                     /locus_tag="VFD74_05425"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HZK49024.1"
                     /translation="SAAAQEVVEKRVDLSRFPISRVGLDEGGRYMTAPVVIAKDPDTG
                     VRNACIVRWMVTGRDRLTFLLDMGRHLRDYYERAEARGEPLQVTINVGVSPAVHFAAV
                     VPPSAAPIDTDELGIAGHLLGRPLELVPGRTVDVEGVASAQFILEAELLPHVREAEGP
                     AAEVTGYYAERADRWVARVTGITRREQPLWHTIIPGQEVYNAVGLVAEASIFRLVSRQ
                     IPGLLGVRLSYGGCGFYHAVVQLQKNVEGIQRNAILATFAAFPSLKQVTVVDEDVNIH
                     DPQDVEWALATRFRPDRDIILIPEARGHELNPITDGGIGCKVGFDATAPTPRLRQFER
                     LAFKTVDLADYDIAE"
     gene            1275..1688
                     /locus_tag="VFD74_05430"
     CDS             1275..1688
                     /locus_tag="VFD74_05430"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HZK49025.1"
                     /translation="MKWKVTPVLMMLAVATAAMGLAACGSDSKPLTLTKDAGGGSAEI
                     KVGQLVEVSLDGNPTTGYSWEVKQSGEPVLRPQGEPAYVQAETDGTVVGGGGTYTFTF
                     VGSEAGTATLELIYHRSWETDVAPLETFTLEVTVK"
     gene            complement(2142..>2695)
                     /locus_tag="VFD74_05435"
     CDS             complement(2142..>2695)
                     /locus_tag="VFD74_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK49026.1"
                     /translation="VAYAALPLTDISGHWAENAIVSMFNAGVVQGRVDGTFHPDDSIT
                     RAEVATMFDRVATNDAAPIDARRGCTDCHVGNYSLKNEAVSRGGAVHDVLSDDIGVDA
                     CLFCHGPAADGKGANAPLSLRDIVHPAHMGSKIFVDHYMGNCFTCHNVDYEGTFQVLG
                     VAVDTDAGGIPDTVPIPGALDPK"
BASE COUNT          468 a          898 c          898 g          431 t
ORIGIN      
        1 cctccgctgc ggcccaggag gtggtggaga agagagtgga cctgagccgc ttccccatca
       61 gccgagtggg cctcgacgag ggcggccggt acatgacggc ccccgtcgtc atagcgaagg
      121 accccgacac cggggtgcgc aacgcgtgca tcgtacgctg gatggtgacc gggcgcgatc
      181 ggctcacctt ccttctggac atgggccgac atctccgcga ctactacgag cgggccgaag
      241 cccgcggtga gcctctgcag gtgaccatca acgtcggcgt cagccccgcc gtgcacttcg
      301 ccgccgtcgt gccgccgagt gccgccccca tcgacaccga cgaacttggg atcgccggcc
      361 atctgctcgg ccggccgctc gagttggtgc cgggccgcac cgtcgatgtg gaaggcgtgg
      421 cgtcggctca gttcatcctg gaggcggaac tcctccccca cgtgcgcgag gcggaagggc
      481 ccgcggcgga ggtgaccgga tactacgctg agcgcgccga tcgctgggtg gcgcgcgtca
      541 cgggcatcac gcgccgcgag cagccgctgt ggcacaccat cattcctgga caagaggtct
      601 acaacgccgt gggtcttgtg gcggaggcca gcatcttccg gctggtgtct cgccagatcc
      661 caggcctgct cggcgttcgc ctctcctacg gcgggtgcgg cttctaccac gccgtcgtgc
      721 aactccagaa gaacgtcgag ggcatccaac ggaacgccat tctcgccacg ttcgctgcct
      781 tcccctcgct caagcaggtg accgtcgtcg acgaagacgt gaacatccac gacccgcagg
      841 atgtcgagtg ggcgctggcc acgcgcttcc ggcccgaccg cgacatcatc ctcatccccg
      901 aggcgcgggg tcacgaactc aaccccataa ccgacggcgg catcggctgc aaggtcggct
      961 tcgacgcgac cgcgcccacg ccgcggctgc ggcagttcga gagactggcc ttcaagaccg
     1021 tcgacctcgc ggactacgac atcgcggagt agggttaggg ggtccgctcc catcgaccct
     1081 gacgacccca aattggctct accccgcggt ctatggggtg cctctgaccg cgctcgctct
     1141 gctcttggcg cgccggtcgt ccttccggcc gtcgcagcat gcgaacgaga ggccggcgcc
     1201 ggcgacttag gaaccgggca cacgtcttcc gcgtcccagg ccgtggcagc gatctggaaa
     1261 gacaggagga ttccgtgaag tggaaggtaa cacccgtgtt gatgatgctg gcggtcgcga
     1321 cggcggccat gggattggcc gcgtgtgggt ctgactcgaa accgctgacc ctcaccaagg
     1381 atgccggtgg aggatctgcg gagatcaagg taggccaact cgtcgaggtg tctcttgatg
     1441 ggaatcccac cacgggctac tcctgggaag tgaagcagtc gggcgaacct gtactacgac
     1501 cgcagggaga gccggcgtac gtccaggccg agaccgatgg cacggtggtg ggcgggggcg
     1561 gcacctacac gttcacgttc gtgggatccg aggcgggcac cgcgaccctc gagttgatct
     1621 atcaccgctc gtgggagacg gacgtcgccc cgctcgagac gttcacgctc gaggtgacgg
     1681 tcaagtagac catcgagtcc cgccgacccg gaagcccggg tcggcgggtt cgtacgggtg
     1741 ggcggcgtac ccgtgcagca tccgcgccag ttccagctga ttgctcggag atgatgtagc
     1801 tgccggacat ccgtccccgc ggatcgcgac ccggttcacc cacatggatc ctcctatcgg
     1861 gcggggatgc gcaaatcaga ctacccggcg gcagccagcg ggacaagcga aggtcccggc
     1921 cggggtcaga ggcgccgcct ttcgcagggg ctgagccgga gacgccaacc caccttgcgc
     1981 tagatcgacc ggccgcagcc ggtcgatcag aacgaggcgc ggtccgaacg gccccccccg
     2041 gaagagcaac gagtacagca gcgaggggcg cccccgacgg gggccgcacc ggcggcctcc
     2101 cgtcgggggc gctatcgact acgcgtcaag ccgattcgat cctatttggg gtctagggcc
     2161 ccggggatcg gaacggtgtc cggaatgccg cccgcgtcgg tgtctaccgc cacgccgaga
     2221 acctggaagg tcccctcgta gtcaacattg tggcaggtga agcagttgcc catgtagtga
     2281 tccacgaaga tcttgctacc catgtgggcc gggtgcacga tgtcacgcaa cgagagcgga
     2341 gcattggcac ctttgccgtc cgcggccggc ccgtgacaga agaggcaggc gtcgacgccg
     2401 atgtcatccg acaagacgtc atggaccgcc ccacctcggc taaccgcctc attcttgagt
     2461 gagtagttgc ccacgtggca gtcggtacag ccgcgccgcg catcaatcgg ggcggcgtcg
     2521 ttcgttgcga ccctgtcgaa catggtcgcg acttcagccc gcgtgatcga atcgtccggg
     2581 tggaacgtcc cgtcgacgcg accctgaaca acacccgcat tgaacatgct gacgatcgca
     2641 ttctctgccc agtgcccgct gatatcggtt aagggaaggg cggcgtaggc tacag
//
LOCUS       DASRMJ010000199         6552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_341814, whole genome shotgun sequence.
ACCESSION   DASRMJ010000199 DASRMJ010000000
VERSION     DASRMJ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341814"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VFD74_05440"
     CDS             complement(<1..814)
                     /locus_tag="VFD74_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZK49027.1"
                     /translation="MAPSQRDFYEILGVKKTATHDEIRKAYRKLARKNHPDANPNDPA
                     AEDRFKEISTAYEVLSDTEKRKQYDQGPTNVFGRQGFDPRGFQGGQGFGDFADLFGNL
                     FGGGGAGRQRQPQSQKGRDLSVAVNLSFEDALKGVTTKISVPKSVQCHMCGGSGAAPG
                     TRPITCPECKGRGVTSQNQGFFALSQPCHRCGGDGTVVEKPCPTCAGRGVTEALKKYT
                     VPLPAGVKDGTKIRLKGKGEPGVRGGPPGDLYVITRVEESGGFHRRGSDFVID"
     gene            complement(819..1499)
                     /locus_tag="VFD74_05445"
     CDS             complement(819..1499)
                     /locus_tag="VFD74_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZK49028.1"
                     /translation="MTHEKRKIPINHRDPNNHEPEGGADPAVETTVGEGAARISAAEA
                     GQADVTSDEVTSPLVALAAERDGYLDALMRLQAEFENFRRRSQRELGEARNRARTGVL
                     GEFLPVFDNLSRALDAAEHHEEGKVLEGVRLTHSLFADLLRREGVCEIPSLGVPFDPH
                     LHEAVVSLPSDVEEGVVIQVVAKGYTVGESVLRPARVAVSAGVPGGEASASGASTSSA
                     PTPGAEKE"
     gene            complement(1596..2081)
                     /locus_tag="VFD74_05450"
     CDS             complement(1596..2081)
                     /locus_tag="VFD74_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZK49029.1"
                     /translation="MAIVRWDPFRELTSLQSEVNRLFSRVSGGDVAERQSWIPSVDVV
                     ESKDAITLKADLAGMDPNDIRLEVEDNVLTVSGERRFQEEVNEEKYYRIERRYGSFSR
                     SIALPQGADPDRIEAEYENGVLAVTVPKVEAAKPKRISVGVRGSGPTTVEGTGSEKAT
                     D"
     gene            complement(2260..4164)
                     /gene="dnaK"
                     /locus_tag="VFD74_05455"
     CDS             complement(2260..4164)
                     /gene="dnaK"
                     /locus_tag="VFD74_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZK49030.1"
                     /translation="MGKIIGIDLGTTNSAMAVLEGGEPTIIPNAEGGRTTPSVVAFAK
                     SGERLVGTVAKRQAVTNPENTIFSIKRFMGRKFRDVSEEMKIVPYAVVEGPNSDVRVN
                     VKGDQYSPPEISAMILQKLKRDAEAFLGTEVTEAVITVPAYFDDAQRQATKDAGKIAG
                     LNVRRIINEPTAASLAYGLDKEQDHTILVFDLGGGTFDVSVLELGEGVFEVKSTNGDT
                     HLGGDNFDKAIVDWIVAEFKREKGVDLSKDRSALQRLYEAAEKAKVELSSMVETTINQ
                     PFITMDESGPLHLELAMTRAKLEELTSDLLDRVVAPTKQAMKDAGMTMGQIHQVVMVG
                     GMTRMPAVMQKVRELTGKEPHKGINPDEVVALGAAIQGGVLAGDVKDVLLLDVTPLSL
                     GIETKGGVFTRLIERNTTIPTRKSEIFSTADDSQTSVEIHVLQGERDLAMYNKTLGKF
                     QLMGIPPAPRGIPQIEVAFDIDANGIVHVSAKDMATGNEQKIEIKSSSGLTDEEIQKM
                     VKDGEAHADEDRAKKQAVETKNQAENLVYSTRKSLKEIGDKVDEATRKTIEDAASDLE
                     EALKGDDVNAIQTKMNTLQEAAYKLSEAAYQAAQGQAGGSGEPGGNGAAGSTDDEVVE
                     DAEYEVIDED"
     gene            complement(4251..4454)
                     /locus_tag="VFD74_05460"
     CDS             complement(4251..4454)
                     /locus_tag="VFD74_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49031.1"
                     /translation="MMVRRPGSPCPSLSAGVDDQIELLQRRLARERAARKQAEQIADE
                     KSRELYINGQDLERTALSSTPLR"
     gene            complement(4602..4853)
                     /locus_tag="VFD74_05465"
     CDS             complement(4602..4853)
                     /locus_tag="VFD74_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49032.1"
                     /translation="MSPLDAASGRGEEGESVSWCDLSEKDARFMVELLQEADRGLDEL
                     RTYQLSRLMSLDPRVQIVDDELKVIQEKVVAALSKLTRR"
     gene            4984..>6552
                     /locus_tag="VFD74_05470"
     CDS             4984..>6552
                     /locus_tag="VFD74_05470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZK49033.1"
                     /translation="MSNGSTNRGSNIQRRSRDNRRRRRAQGRGVGQALTVALVLTPFL
                     LLVLGLGTFTLGLRTAAAVQKDVPRLEDQQQVVLAETSRIYAADGELLAYLHGVENRT
                     LIGGERIPELVKDAVVAIEDERFYEHNGVDLQGVARASLVNLEAGQIQEGFSTITMQL
                     VGNLYLDRRDVSLTRKFREMTLAWQLETIMSKDEILDQYLNTIFFGANAYGIQAAART
                     YFDKDPKDLTLAEAALLAGLPQAPTAYSPRIHSREALDRRNVVLARMYSNGFITYDQY
                     LAAVAQKLVLAPVSPYTAVQEPYVVAFVRKQLIDMFGEDMVFKGGLTVETTIVPRYQQ
                     LAQEAIATTLNLEGDPSAALVAIETRTGFIRAMVGGSDYDVSKFNLAAQGRRQPGSAF
                     KTFALVGAVEMGIDPYNTYYVSQPLELAIPGSTEPWKVKTFGNSYYGSTSVAQATLRS
                     DNTVYAQLALDVTAERIVDVAYRMGITSTLNANPAIALGGLTHGVSPLEMASAYATLA
                     NAGRHRQPTAILKVT"
BASE COUNT         1027 a         2274 c         2024 g         1227 t
ORIGIN      
        1 cgtcgatgac gaagtcggag ccccgccgat ggaacccgcc gctctcctcg acgcgtgtga
       61 tcacgtagag atcgcccgga ggcccgccgc gcactcccgg ctcccccttg cccttgagcc
      121 ggatcttcgt gccgtccttc acccccgccg gcagcggcac cgtgtacttc ttgagcgcct
      181 cggtgacgcc gcgccccgcg caggtggggc atggcttctc gaccacggtg ccgtcaccgc
      241 cgcagcgatg gcacggttgc gagagggcga agaagccttg gttctggctg gtgacgcccc
      301 ggcccttgca ctcgggacag gtgatcggac gagtgcccgg agccgcaccg gaccccccgc
      361 acatgtgaca ctgcaccgat ttcggcacgc tgatcttcgt cgtgacaccc ttgagggcgt
      421 cttcgaacga caggttcacg gccacgctca ggtcgcgacc cttctgagac tgcggctgcc
      481 gctgtctgcc tgcgccccca cctccaaaga gattgccgaa caggtcggcg aagtccccga
      541 agccctggcc gccctggaag ccacgcggat cgaacccctg ccgcccgaag acgttcgtgg
      601 gcccctgatc gtactgcttg cgcttttcag tatcggagag aacctcgtag gcggtggaga
      661 tctccttgaa acgatcctcc gcggccgggt cgttggggtt ggcgtcgggg tggttcttgc
      721 gggcgagctt gcggtaggcc ttgcggatct cgtcgtgagt ggcggtcttc ttgacgccaa
      781 ggatctcgta gaagtctcgt tgagagggag ccacgaccct actccttctc cgcacccggg
      841 gtgggcgcac tcgaagtaga cgccccagag gcggacgcct ccccaccggg gacccccgcg
      901 gatacggcca cacgtgcggg tctgagaacg ctctcgccca ccgtgtagcc cttggcgacc
      961 acctggatca ccacgccctc ctcaacgtcg gagggcaatg acacgacggc ctcatgcaga
     1021 tgcgggtcga acgggacccc cagggatggg atctcgcata cgccttcccg gcgcaggagg
     1081 tcggcgaaca gcgagtgagt cagccgcacg ccttcaagga ccttgccttc ctcgtggtgc
     1141 tcggctgcgt cgagcgcccg cgacaagttg tcgaacaccg gcaggaactc accgagtacg
     1201 cccgtgcgcg cccggttgcg ggcttcgccc aattcgcgct gggagcgccg acggaagttc
     1261 tcgaactcgg cctggagccg catcagcgcg tcgagatagc cgtccctctc ggcagccagc
     1321 gccaccagcg gtgacgtcac ctcgtccgag gtcacatcgg cctgtccggc ttccgccgcc
     1381 gaaatccgcg ccgcgccctc ccccacggtc gtctcgaccg caggatccgc gccgccctcg
     1441 ggctcgtggt tgttggggtc gcggtggttg atggggatct tcctcttctc gtgggtcatc
     1501 tgatcgctat cccttcgcgg ccgtcgtgcc cggaccatcg cggctgccgc caccgaaggc
     1561 ggcagccgcg tccagcccag gatctgcccg gggtgtcagt ccgtggcctt ctcggagcct
     1621 gtgccctcca cggtcgtggg gcccgagccc ctgacgccca cggagatccg cttcggcttg
     1681 gccgcctcga ccttaggcac ggtcacggcc aacacgccgt tctcgtactc ggcctcaatc
     1741 ctatcgggat cggcgccttg cggcagcgcg atactccggc tgaacgagcc gtaccgccgc
     1801 tcgatgcggt agtacttctc ctcgttcacc tcttcctgga agcgacgctc gccactcact
     1861 gtgaggacgt tgtcttcgac ctccaggcgg atgtcgttcg ggtccatgcc ggccaggtcg
     1921 gccttgagcg tgatggcatc cttgctctcg accacatcga ccgacgggat ccaggactga
     1981 cgctctgcca catccccgcc gctgacgcgg gagaacagcc tgttgacctc gctctgcaga
     2041 gacgtcaact cgcggaacgg gtcccatctc acgatcgcca tcgtagtgtc ctccttcctt
     2101 gccactgttt ccttcggtcc gcccgcagcg ccgcggcgat gccgcgacct gcgcgcgaaa
     2161 cgctcgggtg gcggggcggg cgaccccgcc ccgccaccaa accgtccgcg gccggcgccg
     2221 ccggggcgca gccccgcgcg gcccggcccg ccgggacggc tagtcctcgt cgatcacctc
     2281 gtactcggcg tcctcgacca cttcgtcatc ggtcgagccc gcggcaccgt tgccgccggg
     2341 ctcaccggag ccgcccgcct gaccctgagc tgcttggtag gccgcctcgg acagcttgta
     2401 cgcggcctcc tgcagggtgt tcatcttggt ctgtatcgcg ttgacgtcgt cacccttgag
     2461 ggcttcctcg agatcggagg cggcgtcttc gatggtcttg cgcgtggcct catcgacctt
     2521 gtcgccgatc tctttgagcg acttgcgcgt ggagtacacc aggttctcgg cctggttctt
     2581 ggtctccaca gcctgcttct tcgcgcggtc ctcgtcggcg tgggcttcgc cgtccttcac
     2641 catcttctgt atctcttcgt cggtgagtcc actcgagctc ttgatctcga tcttctgctc
     2701 gttgccggtg gccatgtcct tggcggacac gtgcacgatg ccgttcgcgt cgatatcgaa
     2761 cgccacctcg atctgcggga tgccgcgtgg tgccggcggg atacccatga gctggaactt
     2821 gcccaaggtc ttgttgtaca tcgccaggtc acgctcgccc tgcagcacgt ggatctccac
     2881 cgacgtctga ctgtcgtcgg cagtggagaa gatctcactc ttgcgagtgg ggatggtggt
     2941 gttgcgctcg atcagccgcg tgaacacgcc acccttggtc tcgatgccca gagacagcgg
     3001 ggtcacatcc aagaggagca cgtctttcac gtcgcccgcc agtacaccgc cctggatggc
     3061 tgcacccaac gccaccacct cgtcggggtt gatgcctttg tgcggttcct tgccggtgag
     3121 ttcgcgcacc ttctgcatga cggcgggcat acgcgtcatc ccacccacca tgaccacctg
     3181 atggatctgg cccatcgtca tgccggcgtc cttcatggcc tgcttggtgg gagccaccac
     3241 acggtcgagc aggtcgctcg tgagttcttc cagcttcgcc ctcgtcatgg cgagttcgag
     3301 gtgcagcggg ccgctctcgt ccatggtgat gaaaggctgg ttgatggtgg tctcgaccat
     3361 cgaggagagt tccaccttgg ccttctcggc cgcttcgtac aggcgctgga gagcggaacg
     3421 gtccttggac agatccacgc ccttctcgcg tttgaactcg gccacgatcc agtcgacgat
     3481 ggccttgtcg aagttgtcgc cgccgaggtg ggtgtcgccg ttggtgctct tcacctcgaa
     3541 gacaccttca ccgagctcga ggacagagac gtcgaacgtc ccgccgccga ggtcgaagac
     3601 gaggatggtg tggtcctgct ccttgtcgag gccgtaggcc agcgaagccg cggtgggctc
     3661 gttgatgatg cggcgtacgt tgagaccggc gatcttgccg gcgtccttgg tggcctggcg
     3721 ctgggcgtcg tcgaagtagg ccgggacggt gatgaccgct tcggtgacct cggtcccgag
     3781 gaacgcctcg gcgtcgcgct tcagcttctg caggatcatg gccgagatct cgggcgggct
     3841 gtactggtcg cccttcacgt tcacgcgcac gtcgctgttc ggaccttcga ccacggcgta
     3901 ggggacgatc ttcatctctt cggatacatc cctgaacttg cgccccatga agcgctttat
     3961 cgagaatatg gtgttctcgg ggttcgtgac cgcctgccgt ttggcaacgg tgcccaccaa
     4021 ccgctcgccg ctcttggcga acgcgaccac cgaaggcgtc gtgcgacccc cttcggcgtt
     4081 cgggatgatt gtgggttccc cgccttccaa cacggccatg gcggagttgg tcgtgccaag
     4141 gtcgatgccg attatcttgc ccatcgaaat cctccgaaat cgtcggtgcg cgtcgaatcg
     4201 cgcggataga gtatgagtgc agtataaaat ctgagtgcgc taatgtcaag tcaccgcagc
     4261 ggggtggaac tcagcgccgt gcgctcgagg tcctggccgt tgatatagag ttcgcggctc
     4321 ttctcgtcgg cgatctgctc cgcctgcttg cgggcggcac gctccctcgc cagccggcgc
     4381 tgcagcaact cgatctgatc gtccacgccg gcgctcagcg aggggcatgg tgaaccgggt
     4441 ctccgcacca tcacccatga cggtctccct gctcacggcg agggcctccc cgtagtggcc
     4501 gatgcacgcg aggatcaacc cttcggccag gtcggccagg ttgcggctca gtgggagcgc
     4561 gggcggtccg gggcgcgcgg gcggtcgaac gggcggcggc gctacctccg agtgagcttg
     4621 gacaaggcgg ccaccacctt ctcctggatc accttgagtt cgtcatccac gatctggacc
     4681 ctcgggtcga gtgacatgag gcgagagagt tggtaggtgc gaagctcgtc aagcccacgg
     4741 tcggcctcct gaagcagctc caccatgaac ctggcatcct tctccgatag gtcacaccac
     4801 gacacggatt cgccctcctc acccctgccc gacgccgcgt cgagaggact catcgaccat
     4861 cctggaatca tactggaatg caggcttctc cgggcatcag cggtccgatg ttcaccgccg
     4921 caccgaagcg accggcggcg gcggagaccc gagaccgctc gcttcggcta caatacggac
     4981 accatgtcca acggcagcac caaccggggc tccaacatcc agcggcgctc gcgcgacaat
     5041 aggcgcaggc ggcgcgctca aggccgcggt gtggggcagg ctctgaccgt ggccttggtg
     5101 ctcactccgt tcctccttct cgtgctcggc ctcggcacgt tcaccctggg actgcgcacg
     5161 gccgcagcag tccagaaaga cgtgccccgc cttgaggatc agcaacaggt ggtgctcgcc
     5221 gagacatccc gcatctacgc cgccgacggc gagttgctgg catacctgca cggcgtcgag
     5281 aaccgcaccc tcatcggcgg ggagcggatc cccgagctcg tcaaggacgc cgtggtggcc
     5341 atcgaggacg agcgcttcta cgagcacaac ggggttgacc ttcagggtgt cgcccgtgcc
     5401 tcactggtca atctggaggc cggacagatc caggaaggct tctccacaat cacgatgcag
     5461 ctcgtgggca acctctacct cgaccggcgc gacgtctccc tcacccgtaa gttccgcgag
     5521 atgaccttgg cctggcagct cgagaccatc atgtccaagg acgagatcct cgaccagtat
     5581 ctcaacacca tcttcttcgg ggccaacgcg tacggcatcc aagccgccgc gcgcacctac
     5641 ttcgacaagg atccaaagga cttgacgctc gcggaggcgg ccctcttggc cggcctgccg
     5701 caggcgccca ccgcctactc gccgcgcata cactcgcgcg aggcgctcga tcggcggaac
     5761 gtcgtgttgg cgcgcatgta cagcaacggg ttcatcacct acgaccagta cttagcggcg
     5821 gtcgcgcaga agctcgtgtt ggcgcccgtc tccccgtata cggccgttca ggagccctat
     5881 gtcgtggcgt tcgtccgcaa gcagctcatc gacatgttcg gcgaggatat ggtgttcaag
     5941 ggcggcctga ccgtggagac caccatcgtt ccccgctacc agcagttggc tcaggaggcc
     6001 atcgcgacga ccctcaacct cgagggcgac ccctctgccg ccctggtggc catcgagacc
     6061 cgcaccggct tcatccgagc gatggtgggc ggtagcgact acgacgtgtc caagttcaat
     6121 ctcgccgccc aaggccggcg tcagcccggt tcggcgttca agaccttcgc cctcgtaggc
     6181 gcggtggaga tggggatcga cccgtacaac acctactacg tgtcccagcc tctggagctc
     6241 gccatccccg gcagcaccga accctggaag gtcaagacgt tcggcaacag ctactacggc
     6301 agcaccagcg tggcccaggc gaccctgcgc agcgataaca ctgtgtacgc gcagctggcg
     6361 ctcgacgtca cggcggagcg catcgtcgac gtcgcctacc gcatgggaat caccagcaca
     6421 ctgaacgcga accctgccat cgcgctcggc ggcctcaccc acggcgtctc cccgttggag
     6481 atggcttccg cgtacgcgac cctggcgaac gcgggccggc accgccaacc caccgccatc
     6541 ctcaaagtca cc
//
LOCUS       DASRMJ010000200         4569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_377300, whole genome shotgun sequence.
ACCESSION   DASRMJ010000200 DASRMJ010000000
VERSION     DASRMJ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377300"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFD74_05475"
     CDS             <1..183
                     /locus_tag="VFD74_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49034.1"
                     /translation="GLVAGLNPLVRLRYECKRSRGDARCVGVYTWKVPPLRPSPTLQP
                     GPAAASELAAQAHSSL"
     gene            complement(246..617)
                     /locus_tag="VFD74_05480"
     CDS             complement(246..617)
                     /locus_tag="VFD74_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZK49035.1"
                     /translation="MRLLIRWGINTIAIMAAAYLLPQVHLTGWKAALVAGVLLGFLNT
                     FVRPVFRLLALPLTILSLGLFVLVVNGFVLWILDWLMEGLKIDGFIWDIAAALIISVV
                     TTIVNVVTGAGKAGKKRRGRG"
     gene            complement(689..1663)
                     /locus_tag="VFD74_05485"
     CDS             complement(689..1663)
                     /locus_tag="VFD74_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZK49036.1"
                     /translation="MPDAPIHPERTARALSLLSYLLAEERGDSVPLASVMRDLGVTRK
                     QVAEDLSLLNLVNHGGGTYVIYGELTDDTIEVTREPAGDAMASPARLSPLMARALLLA
                     VDLVGDKLPVDGRRTLTSIRDKVKKSIEGTDLPGLVEIETASAEVTDIIEVLNHGLRE
                     RMVVDLEYYTPAREELGRRQVEPYLLFDSNDAWYLEAYCRRVEAQRTFRIDLIRSAAL
                     TGEHFEPRASVDLSARRARALSVVPQHPRWAVVRFPVTRARSLEEQGLEVTRMEDGTV
                     EARIPYLDERWLVREILRYAGEAVIVSPENLRRQVVEETRVIRSSYES"
     gene            complement(1656..2672)
                     /locus_tag="VFD74_05490"
     CDS             complement(1656..2672)
                     /locus_tag="VFD74_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZK49037.1"
                     /translation="MPKDDDKLIRQLSLVSFLLAQRHPVTPRHIHERVEGYAGMSDST
                     FARRFYDDRSDLEQVGIAIEGADPEDEGEAYFLPEENYRLSSLDLTAEELRALAVALT
                     LLEGRFAYARPLRLALVNLTHGHPDPESAELERIAVSLAPDEEARSLGPALAQLDNAI
                     ARGKTVRFLYPTGPDLEPQERVVDPYGLSRIGGHWYAGGRDHERDALRTFRLSRFEGP
                     VRFATKRARDFSPPMGIDPADFRARPPWQLGETVGTATVRVEDDLAWRVARAYPGVAA
                     ESWPGPLDLTDSPATLFTTPYADADALITWVLSLGRKAELLGPPELRSLVREKLLALE
                     RAHA"
     gene            2784..>4569
                     /locus_tag="VFD74_05495"
     CDS             2784..>4569
                     /locus_tag="VFD74_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49038.1"
                     /translation="MISAPPTATYAPGLHLVLGPPNAGKLGLALRWWVGLREARPLLV
                     VPSLADAADVESELLERAGVVFDDRPVCTIDRLVSLLVPEGPSLLGDLRRSLIIAEIL
                     ARGLPGPLGALIGHPGTLGAVCGLIDEFGESGHTSDAAAAALRRASAAGGGDAMDDLA
                     AVLRDYDTAAALRGRVDRHQALAAATSSCEGWVRPVGFHGFLSFTAAQRRLVLALAEV
                     VPVLVTLTVSPDPGVAGYAAEEAAFLQEAALDRAAAGIVLRMPRQEFSFASPAIAHLE
                     RSFLAPDPGPLPAAEADLRIQERGRQHEGVRFLLSAGRRSEVESVAAEIVALLREGTK
                     PDDIAVLVREIGPWQRTVREVFGRFGVPHRVDGLTSLGATGLGQAVLMALRGVASHDL
                     DSLLAYLRTPYHPATLSAVDSTEVLLRKKAVVSGLRVTEEFDRLLPGALDLARAAVIL
                     RGEKAVGVSEPALGALSATMVATAARQRSLGSAALDEDARALAALIGALRETAQEAAE
                     GGVSQLSAGSRAVDGAVEDGPVTDAAVVDAASGRLPVVGGPSETTVSGTPVPTRSFDL
                     ALSLLSSFPVPLGVGDEVGVVQVTSVRRA"
BASE COUNT          691 a         1509 c         1582 g          787 t
ORIGIN      
        1 ggtctggtcg ccgggctcaa cccattggtg cggctccgct acgagtgcaa gcggagccgc
       61 ggcgacgccc ggtgcgtcgg tgtgtacacc tggaaggtcc cgcccttacg gccgtccccg
      121 acgctgcaac ctgggcccgc ggcagcctcc gaactcgcag cccaggccca cagcagcctc
      181 tgaggtcgca gcctagcgct gaggcagccg ccgattcgtc ggggtctttg gccgggcccg
      241 cttcgctacc cgcgcccgcg gcgcttcttc ccggcctttc cggcgccggt gacgacgttg
      301 acgatcgtgg tgactaccga gatgatcagc gcggcggcta tgtcccatat gaacccgtcg
      361 atcttgagcc cttccatcag ccaatcgagg atccagagga cgaacccgtt gacgacaagc
      421 acaaagaggc cgagcgacag gatggtgagg ggtagcgcga gcagacggaa caccggtctc
      481 acgaaggtgt tgaggaagcc cagaagcact ccggctacga gcgcggcctt ccagccggtg
      541 aggtggacct ggggcagcag gtaggctgcc gccatgatgg ctatggtgtt gataccccaa
      601 cgaatcagca ggcgcatgag cttctccttg cccgggtgtg atgttccatc agactcaggg
      661 gcgccaggcg gacgccgccc tatctgcgct acgactcata cgacgagcgg atgacgcggg
      721 tttcctctac gacctgtcgc cgcaggttct ccggtgagac gatcacggct tcgccggcat
      781 agcgaagtat ctcgcgcacc agccagcgct catccagata ggggattcgc gcttcgacgg
      841 tgccgtcctc cattcgcgtc acctccagac cctgctcctc cagggatcga gcccgggtga
      901 ccgggaagcg gacgacagcc cagcgagggt gttgagggac gaccgagagg gcgcgcgcgc
      961 gtcgcgcgct gagatcaacg gaagcgcgtg gctcgaagtg ctccccggtg agcgctgcgg
     1021 agcggatcag gtcgatccgg aatgtcctct gggcttcgac gcgtctgcag taggcttcca
     1081 ggtaccacgc gtcgttcgaa tcaaagagaa ggtagggttc gacctgacgt cgcccaagct
     1141 cctctcgggc cggcgtatag tactcaaggt ccaccaccat acgttcgcgc aggccgtggt
     1201 tgagcacctc gatgatgtcg gtcacctcgg ccgatgcggt ctcgatctcg accaggcccg
     1261 gtagatccgt cccttcgatg ctcttcttga ccttgtcccg gatcgaggtg agcgtccggc
     1321 gtccgtccac cgggagcttg tcgcccacga ggtccacagc caagagcagg gcgcgcgcca
     1381 tgagcggaga gaggcgggcg ggggaagcca tagcgtcccc cgccggctcc cgcgtcacct
     1441 cgatggtgtc gtcggtcaac tccccataga tgacgtacgt ccccccgccg tgattgacga
     1501 ggttgagcaa agagaggtcc tcggccacct gcttgcgtgt gacgccgagg tcccgcatca
     1561 ccgaagcgag cggcacggaa tcgccccgct cctcggcaag gaggtagctc agcagcgaca
     1621 gggcccgagc cgtgcgttcc ggatggatcg gagcgtcagg catgtgccct ctccagcgcg
     1681 aggagctttt cgcgcaccag gcttcggagt tcgggcgggc ccagcaactc cgccttgcga
     1741 cccaggctca ggacccaggt gatcagggcg tcggcgtcgg cgtagggagt ggtgaacagg
     1801 gtggccgggg agtcggtgag gtcgagcggt cctggccacg actcggccgc cactccgggg
     1861 taggcgcgcg ccacgcgcca ggccaggtca tcctccaccc gcacggtggc ggtgcccaca
     1921 gtctctccca gctgccacgg cggtcgcgcc ctgaagtcgg ccggatcgat gcccatggga
     1981 ggtgagaagt ccctcgcgcg cttcgtggcg aacctcaccg gcccctcgaa gcggctcagg
     2041 cggaaggtgc gaagggcatc ccgctcgtga tcacgtccgc cggcgtacca atgccccccg
     2101 atccgagaga gaccgtacgg gtcgacgacc cgctcctgcg gctccagatc aggcccggtg
     2161 ggatagagga atcgcaccgt cttcccgcgc gcgatggcgt tatcgagttg cgcgagcgcc
     2221 ggccccaggg agcgcgcctc ttcgtcggga gcgaggctca cggcgatgcg ttcgagttcg
     2281 gcgctctcag ggtcagggtg gccgtgggtg aggttcacga gggccaatcg cagcggccgg
     2341 gcgtaggcga agcggccctc caacagggtc agtgccacag ccagtgcgcg caactcctct
     2401 gcggtgaggt caaggctgga gagtcgataa ttctcttcgg gaaggaagta ggcctccccc
     2461 tcgtcttcgg ggtcggcgcc ttcgatggct atgcccacct gctcgaggtc ggagcgatcg
     2521 tcgtagaagc ggcgggcgaa cgtggagtcg gacatgccgg cgtatccctc gacccgctca
     2581 tggatgtggc ggggcgtcac cgggtgtctc tgcgccaaaa ggaacgagac gagggagagt
     2641 tggcgtatga gcttgtcgtc gtccttaggc atcagcttag agtatcgcac ccacccgacc
     2701 tatcgccacc gatactgagt gcggcgtcgg ctcgacgtag cactcgtgac ggttccgtcc
     2761 ggagcgtgag ctacaatcgg ccgatgatct ctgcgcctcc gaccgcgacg tacgcccccg
     2821 gactccacct cgtcctgggc cctcccaatg ccgggaagct cgggcttgcg ctgagatggt
     2881 gggtcggatt gcgcgaggcg cgtccgctgc tcgtcgttcc ctcgctggcc gatgccgccg
     2941 acgtcgagtc cgagctgctc gagcgtgcgg gtgtggtctt cgacgatcgt ccggtatgca
     3001 ccatcgatcg gctggtctcg ctgctggtgc cggagggtcc gtctctcctg ggggatctcc
     3061 ggcgctcgct gatcatcgcc gagattcttg cgagagggct gccggggccg ctgggagcct
     3121 tgatcggtca cccgggcacg ttgggcgccg tgtgtggcct catagatgag ttcggcgaga
     3181 gtgggcacac gtccgatgcc gccgccgccg ctttgcgccg tgcgtcggcg gccgggggcg
     3241 gcgatgccat ggacgatctc gccgcagtac ttcgtgacta cgacacggcg gcggcgctcc
     3301 gcggccgggt cgatcgtcat caggcgctgg cggcggcgac ctcctcgtgc gaaggctggg
     3361 tgaggccggt cgggttccac ggcttcctct ccttcaccgc ggctcagcgc aggcttgtcc
     3421 tcgcattggc cgaagtcgtc cccgttctgg tcaccctgac ggtgagcccg gatccgggag
     3481 tcgcggggta tgccgccgag gaggccgcgt ttctccagga ggccgccctg gatcgcgccg
     3541 cggcgggcat cgtgctgcgc atgccgcggc aggagttctc gttcgcctcg ccggcgatcg
     3601 cccacctcga gcgctccttc ctggccccgg accctgggcc tctgccggcg gccgaggccg
     3661 accttcggat ccaagagcgg ggcagacagc atgaaggggt gcgattcctt ctttcagccg
     3721 ggagaaggag cgaagtggag tcggtggcgg cggagatagt cgccctcctg cgtgagggga
     3781 cgaagcccga cgacatcgcc gtcctggtgc gtgagatcgg cccttggcag agaacagtgc
     3841 gtgaggtctt cggtcgattc ggcgtgcctc atcgcgtgga cgggctgacg tcactcggtg
     3901 ctacgggact cggccaagcc gtgctgatgg cgctccgggg tgtcgcgagc cacgacctag
     3961 actccctcct ggcgtatctg cgaacgccct atcatccggc gaccctgtcc gcggtcgatt
     4021 ctaccgaggt cttgttgcgg aagaaggcag tcgtctctgg cctccgggtc accgaggaat
     4081 tcgaccgcct gctgcccggt gcgctggacc ttgcgagggc cgccgtgatc ctgagaggag
     4141 agaaggctgt gggcgtctcc gagccggcgc tcggcgctct ctccgcgacg atggtcgcca
     4201 cggccgcccg acagcgatcc ctcggctctg cggccctcga cgaagacgct cgagccctcg
     4261 ccgccctgat cggggctctt cgggagaccg cccaggaggc ggccgaaggg ggagtctcgc
     4321 agctgtcggc cggtagcagg gccgtggacg gtgcagtcga ggacggtcca gtcaccgacg
     4381 ccgcagtcgt cgacgccgcg tcgggccgcc tcccggtcgt cggcggtccc tccgaaacta
     4441 cggtctccgg gacgcccgtc cccacccgat cgttcgatct tgcgctttcg ctcttgagca
     4501 gctttcccgt gcctctcggg gtcggggacg aggtcggggt cgtccaagtg accagcgtca
     4561 ggcgtgcgc
//
LOCUS       DASRMJ010000201        19910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_304775, whole genome shotgun sequence.
ACCESSION   DASRMJ010000201 DASRMJ010000000
VERSION     DASRMJ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 19910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19910
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304775"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..428)
                     /locus_tag="VFD74_05500"
     CDS             complement(51..428)
                     /locus_tag="VFD74_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK49039.1"
                     /translation="MKPRRLADYIGMTIRRLRMEREYTLQALADESGISKSYLGDIEK
                     GRKNPTTDVVEAVASALGVPARRLLYHAALDEEDPFFEPEQLILEADTEEQTEVRELS
                     HLAHRLRPVDRRILVELARRLAR"
     gene            664..1935
                     /gene="larC"
                     /locus_tag="VFD74_05505"
     CDS             664..1935
                     /gene="larC"
                     /locus_tag="VFD74_05505"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HZK49040.1"
                     /translation="MEQGRAGRILYLDPWSGVSGDMLLGALLDLDRDGRLEWALREAV
                     GALGLPEAGVEVGRGTSGGIACTRVDVAVSGTPPLRSLSDLQNLLEESGLAEAVKTRA
                     FRALARLASVEAGIHGVTVEQVHLHELGAVDTVVDVVGCIALVDALHVDEVVHGPVPL
                     GTGSVVTAHGRLRVPAPATLALLQGVPIFAGPEECEVTTPTGALLLRELAGRCGAMPA
                     MIVEAVGYGGGARTLEHGANVLRAVIGRAASAEPSASAATASSGEPARGRRASEAGLP
                     EFVVELEAVIDDASPEILAYLHQALLSAGALDAWWTPAFMKKGRPGFELTVLCRPVDE
                     VALVETIFRESTTFGVRRSERSRHVLDREWVQVDVDGETVRVKVGRLNGRAVTVAPEF
                     EDAARAAAGLGTPLADVMATAAASARAILGG"
     gene            2260..3921
                     /locus_tag="VFD74_05510"
     CDS             2260..3921
                     /locus_tag="VFD74_05510"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZK49041.1"
                     /translation="MKSDIQIAQEATLKPIIEVARDLGLGQDDIDLYGKYKAKIHLDV
                     LDRLADKPNGKLILCTAITPTPAGEGKTTTNVGLSQGLAKIGKSVVTTLREPSLGPSF
                     GIKGGAAGGGYAQVVPMEDINLHFTGDLHAITTAHNLCAAVLDNHLFQGNDLDIDIEN
                     ITWPRVVDLNDRALRNVTVGQEGEGVERKSRFDITVASEVMAILCLATDLEDLKARLD
                     RVIIAYTKSGTPVTAKDIKVSGSMAVLLKDALMPNLVQTLENTPAIIHGGPFANIAHG
                     CNSVQATKLGLKLADYCVTEAGFAADLGAEKFFDIKCRYADLTPDAVVLVATVRALKM
                     HGGVAFADLKTENLAALEKGLENLQKHVENMQLYGVPVVVAINRFPDDSDGELKLLDE
                     KVKAMGSKVVLSEVWAKGGDGGVELAEEIVRMCEDEKPDFKFLYSLDLSIKEKIEAIA
                     TKIYGADGVDYAPEVEEALELITRNGYSGLPICMAKTQASLSDDKTVVGRPRGWRLTV
                     REARLSAGAGFIVPVCGKMMTMPGLPKRPAAESIDLVDGKIVGLF"
     gene            3961..5919
                     /locus_tag="VFD74_05515"
     CDS             3961..5919
                     /locus_tag="VFD74_05515"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZK49042.1"
                     /translation="MTERYTLTFQPGDVVVADVPRGESVLRAAMQADLHINASCGGSG
                     SCGKCRIQIIAGEVESERSSKLDDDEWAEGIRLACRTRVLSDVEIFIPETSRMGNAAV
                     LERELRGERKGRLLNPAELESLVTGWTLDPVVKKLCMQMTPPSEGDNLNDLARFKKAL
                     KDNFGLAKVSVDSVWIRNMPHVLREHDWQVTATVVDTSYGYKVIELESGSYCDRDFSV
                     VMDIGTTTVWGQLLDLNQGTTLAQASRYNKQISYGDDVISRIVFSQKPNGRETLQQAI
                     VDTINEIVDELVQKSGVEREQITHMVAAGNTTMTCMLVGIDPKYLRMAPYVPPADFIP
                     PVRAVNLGAGIKVGRHVHVHTFPSVASYIGGDIVSGIVGSGIFDSEQITLYIDIGTNG
                     EVVIGNREWLIATSCSAGPAFEGGGIKHGMRATDAAIETVRIDGVTYEPTLLTIGDTK
                     PIGICGSGIIDAVAELLRAGVLSQNGKFDMSLPSPRLRETEGANEFVLVEAEFSGTGD
                     DIVITEADVDNLVRAKAAIFAGIMTLLESVALDWADLEQIYVAGGFGKHLRIEQAQAI
                     GLFPEVDMDKFTFVGNGSLLGARLVSFSKDMLKAAEQVARMMTNVDMSDNQMFMDRYV
                     AAMFLPHTDMTYFPEMEATLAARAGRGR"
     gene            5919..6677
                     /locus_tag="VFD74_05520"
     CDS             5919..6677
                     /locus_tag="VFD74_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK49043.1"
                     /translation="MAATKTVAVAGKGGTGKTTLAGLMIREMVARGISPVLAVDADPN
                     ANLGEILGTKAAVTVGGLREQAFVGAREIPTGWSKQSWIEYKMHEAIEEAHGYDLLVM
                     GRPEGPGCYCYANNLLREYIKTLANEYAWVIMDNEGGLEHLSRHTTRDVDVMFIATDA
                     SVRGARTVERISELADEMGLVIAQRYVVISRAPEGLPVERLMEHTSVPLGGVIPDDPA
                     LFENDLAGGSVFDLPDDAVSLGAVRGLMDRYLDI"
     gene            6757..7392
                     /locus_tag="VFD74_05525"
     CDS             6757..7392
                     /locus_tag="VFD74_05525"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HZK49044.1"
                     /translation="MAKGYIDGSIREFLDKLGTSSPEPGGGSVAALAAALGAGLVSMV
                     ASLTLGKEKYSGVQDDIKVLLGRSEAVREALQDLVQQDTVVYGAVSTAMKLPRDTEEQ
                     RAARDRLMQAALVEAAKVPLAIAEQALAVAQLSLTAGEIGNVNAVSDAGVAVLLADAA
                     AQSAALNVKINIGWIQDRAFNEGSWSRVQEILSETAVLRASVMGLTYEKLG"
     gene            7451..8416
                     /locus_tag="VFD74_05530"
     CDS             7451..8416
                     /locus_tag="VFD74_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006637499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HZK49045.1"
                     /translation="MGAKVIDGKAIAAEIREELVRELEELKSQGIEPGIATLLVGDNF
                     GAKMYRGAVEKFCAEMGFAYREETHSADISEEEVIRIVESLNADPSVSGILPLRPFPE
                     HISDSAVINSISVNKDIDCFHPFNMGKLAVGEPTFPPATPSACIELLERYMKSEGIDP
                     KDGFEGAEICVVGHSNIVGKPVGFLALNRNASTSVVHVFTSMKGNLEKHTTAADYLIV
                     AAGFPNLINAAQVKEGAVVIDVGINRVIVCPVCGAYNRSKKDACKKCAADLAEAETRT
                     VGDVNFDEVVEKAKAITPVPGGVGSVTNMMLAKNTLRAARLTAGA"
     gene            8466..9401
                     /locus_tag="VFD74_05535"
     CDS             8466..9401
                     /locus_tag="VFD74_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HZK49046.1"
                     /translation="MAFQAPNESYKGAIREADIAGVTVGGETAFPFHAFEGEMPNTPK
                     LAFEVWDIAPDDWSSTLNDVYGDVFTDPVAWAKKAVDEFGADMIYLRLRSTDPNGAAR
                     DAAEAAAAAKAVVEGVSVPVIVVGSGDAERDPEVLKTVAAALADHKVVIGNAEEDNYR
                     SVGAAAMGYQHAVVAFSPMDVNLAKQLNVLLAQLGVDDGAILMDPTTGALGYGLEYAY
                     TVFERDRLAALAQNDPKMQMPIIATVGQEAWKAKETKVGENELPGTGDMVTRGLMWES
                     LTSVALLLAGANIVVLRHPESAALIRKAVASLKGE"
     gene            9424..11379
                     /gene="cooS"
                     /locus_tag="VFD74_05540"
     CDS             9424..11379
                     /gene="cooS"
                     /locus_tag="VFD74_05540"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HZK49047.1"
                     /translation="MTDEKKKRGGDYAKSIDPAALEMLQIADEKGISTMFSRVDEVSA
                     CPIGAEGACCKICFMGPCRFVGKDKEEKHGVCGASLATVAARNYIRSCAGGASAHADH
                     GRTIAETLLAVGKGEAADYRVKDPIKLRTVAGYFDIDVEGKDDNQLAVEVATAALNDW
                     SKHNEYQTYVKRAPKKRQELWAKLGVTPRGFDREVVDSMHRTHAGGDQDAPHIMDQAI
                     RMCLASGWGGSMLATDLSDILWGTPKPVTSEANLGVLNIEDVNIIIHGHEPVLSEMIV
                     DASQDPEMIEYAKSKGAAGITLAGICCTANEIWMRRGVPPAGNSLHQELALLTGAVEL
                     MVTDIQCVFQGIVGVAKNFHTHVVTTSQKAHIEGAMRVEFHEDRALEIAKDIVRMAID
                     NFQNRDAAKVHIPNIKSPVVAGFSHEYIRYMLGGKFRGSFQPLNDNIINGKIQGAVAI
                     VGCNNPRSTQDAGIINLVKNFIENDVLVLVTGCAAHAAGKHGYLTPEMFANAGPGLSE
                     VCETTGMPPVLHVGSCVDNSRILTILADIVETGGLGDDIDQIPAVGISPEYYCEKALE
                     IATYCVGSGAYVIFGGVESPVRASGVVTDIHTSGWEKQFGGKLEFISDWEGIFEAAMD
                     HIRKKREALGIHEEHERVLFDMADRRG"
     gene            11385..13601
                     /gene="acsB"
                     /locus_tag="VFD74_05545"
     CDS             11385..13601
                     /gene="acsB"
                     /locus_tag="VFD74_05545"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF007078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HZK49048.1"
                     /translation="MTTMIQKRKPEGIREIVAGGVARGSRAYFDLAKSRFEEAAAGMD
                     TGQKREFFPNTGYFLPVIYAITGHKVECLDDMRWVLDEAERLLPPVPSNNLWLPYLGE
                     TLDGGMASLWLAEIIEALKYVGAGPAPVDGIWLGAADDVIMRARGVEFVDGSAPGFAA
                     VTGAAVDVETAVALAREMQQKSLYVFMAGESRGTTFAEQLAEGGLDMAWETRLVPFGP
                     EIYSHIYSVGFATRAAMAFGGVKPGDFKNILKYNKDRVFAFVMALGEVDDLKYAAAAG
                     AINYGFPTIAETDIPEILPTGVTTYEHVVSNIPTSEIVAKSIEVRGLKIVITEVPVPV
                     AYGPAFEGERIRKEDLYVELAGPKAPGAEFTVIADNVEDGKVTLVGPDVDQIEVGTTI
                     PYAVLIEVSGRKMQKDFEPILERQLHHFLNEAEGFLHVGQRDIIWERISKKAVAAGFK
                     FEHVGKIIHARYHADFGGIMDKVQITIYTQEPEVSELVARAQSAYHERDERIGALVDE
                     SVETFYSCSLCQSFAPDHICVITPERPGLCGAYNWLDGKAAFEISPTGPNQPIAKGVA
                     VDEVKGQWVGVNEFVYQASHNAVERFNAYSMMEDPMTSCGCFEAISVLLPMCNGVMAV
                     DRDHTGMTPCGMKFSTLAGSVGGGAQTPGFVGHSKFYMGSSKYISGDGGVARVVWMPK
                     KLKEELKDAITASADKAGYPGLYEKIATEENGTEEEEVMTYLAEVGHPALEMDPMF"
     gene            13699..15033
                     /gene="acsC"
                     /locus_tag="VFD74_05550"
     CDS             13699..15033
                     /gene="acsC"
                     /locus_tag="VFD74_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HZK49049.1"
                     /translation="MALSGLQIFKLLPKTNCKDCGFPTCMAFAMQLASGKAELSQCPH
                     VSAEAAAELGEAAAPPVRKVTIGTGDFAVVVGEETVLYRHEKRFEHPPGIAILVDDSV
                     DDAEFQVRLDTFKGQVFERVGQLLRAKLFAIESADAGRLAARVKKASDETGAGIVVMS
                     DDPAALKAAAEPIKDRKPLLYAATTATFDAVSAAAKELGVPFAIKAATLDELAEMGEK
                     CVAADLKEVVLDPMSANLQDALRDQTYIRRAAIKKKFRALGFPTIAFPCRMTDEPLLK
                     TVYAGVFVAKYAGIIVIDTVDPAQIMPLLILAQNIYTDPQRPMQMDQGIYPINDPSPD
                     SPLLITTNFSLTYFTLAGEVEASKVPTWLLVMDTEGQSVLTSWAAGKFVADAIAVFVK
                     KSGIAEKVNHRKIVIPGYVAQISGELEEELGDWEVVVGVREAADVPRYLRGL"
     gene            15137..15808
                     /locus_tag="VFD74_05555"
     CDS             15137..15808
                     /locus_tag="VFD74_05555"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZK49050.1"
                     /translation="MIISTQKPFEEILESLKGENSVYIVGCGECATMTQSGGEPEVAD
                     MKARLEEAGKKVVATDVAHAACHELDMKRIFRQNKEAATAADAFLVLACGAGVQSVRE
                     GTTKHVTPGANSLFVGNVKRQMAFEEKCSLCGECVLDEYGAICPVTRCAKGILNGPCG
                     GTNNGKCEVDSNKDCAWVLIYNQMKSEDKLASFMTIHGPKRWNAIERPGRLDARAAGE
                     EKGAA"
     gene            15811..16710
                     /locus_tag="VFD74_05560"
     CDS             15811..16710
                     /locus_tag="VFD74_05560"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZK49051.1"
                     /translation="MSKYREACESGRFVVSGEIGPPKGTSVDTMLHHIELLKDLVDGL
                     NVTDNQSAVMRLGSMAVSHEIVSRGGDAIYQMTCRDRNRLALQNDLLSAHFLGLRNVL
                     ALTGDHVSVGDHKDAKEVYDFESVQLIQCIKRMNEGFDWAGNELEGGTDFYIGAVVTP
                     ESDPIEPQKLKFEKKIEAGAQFFQTQAVYDMDNFRRFMEYARGVTEGKDVKMMAGLVV
                     LTSVGMAKYMNKFVPGVFVPDDLLEEMASVPKEEAVKKGMEICARHIRFCIDNDVCDG
                     VHVMAIGKEEIVPEILQMAGLLK"
     gene            16780..18174
                     /gene="lpdA"
                     /locus_tag="VFD74_05565"
     CDS             16780..18174
                     /gene="lpdA"
                     /locus_tag="VFD74_05565"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZK49052.1"
                     /translation="MAEATRIVIIGGGPGGYVAAIRAAQRGADVTVVEKDLLGGTCLN
                     RGCIPTKALLGSIEALHKARSGGEFGFGVTGEIVPDFTRMMERKSEISKTLRDGIHGL
                     FKKSKVKLVQGEGRIVGPGKVEAATAEGVVTLEADKIIIATGSEPARLPFFDFDQPTV
                     LTSTGALELTKIPKTLLIIGAGVIGCEFATVFSELGTKITIVEMMPQMLPLEDKRLAK
                     QFQGIFKKRGIEVLLKTKVDGIAEYADDHITANIEGGEQITAEKMLVSIGRQPNSAGL
                     GLETVGVETDARGYIVVNDKLETTAPDVYAIGDVNGGILLAHVASYEGLVAVENCLGG
                     NQERDLKVVPSCIYTMPEIASVGLTEDQARDAGFEPISGTFRLGAAGKALAIGEAQGY
                     IQIIADQATDKVLGANMMGPHVTDLIHEIAVAVRNGLTVRDIGDTIHAHPTIGEALME
                     AAHDVHGESVHVAS"
     gene            18218..19144
                     /gene="lipA"
                     /locus_tag="VFD74_05570"
     CDS             18218..19144
                     /gene="lipA"
                     /locus_tag="VFD74_05570"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZK49053.1"
                     /translation="MENDRQVTTGVAEHLGRKPEWLKKPLPKAAAVHGVEGLLRRSSL
                     HTVCESAKCPNKGECFERGTATFLIMGNACTRDCRFCSVPSGRPELLDASEPQHVADA
                     AETMGLSHVVITSVTRDDLADGGAAHFAATIEAVRGRLPGATIEVLVPDFQEDAASLD
                     VVLGARPDVFNHNVETVPRLYARVRPQADYRRSLRVLAYAAAKGTGVVKTGIMVGLGE
                     SVEEVGTFLQDAVAAGVSVVTIGQYLRPSRDHLPVTEYVAPAVFDGYRLAGEALGLVV
                     EAAPFVRSSYKAEEGLRRVSGERVRESREDTC"
     gene            19138..>19910
                     /locus_tag="VFD74_05575"
     CDS             19138..>19910
                     /locus_tag="VFD74_05575"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZK49054.1"
                     /translation="MLVIAENINVMVTRIGNAMKEGDKAPIQDLARRVVEAGADVIDI
                     NLGPARKNGDKMMEFVVETVAEVTRLPFCLDTTNHVAMEAGIVKCLELGLPHPIVNSF
                     SAQPDKMANILPLAAKYDCEIIGLTMGAAIPIDANERVALAYELVMAANEQGISNERI
                     FIDPIILPVGVDVGQQHAVAVQEVLKMLPEMFDPMVKTTCGLSNISNGAPDTMRPAIN
                     NVFVAMLAAVGMTSAIVDVLDKEMMRSIRLIKALTNQSL"
BASE COUNT         3785 a         6046 c         6702 g         3377 t
ORIGIN      
        1 gggtgacggg cggtcctgcg tcagccggtg gggtgacggg cggtcccgcg tcagcgcgcg
       61 aggcgacggg cgagctcgac aaggatcctg cgatccaccg gtcgcagtcg gtgcgccagg
      121 tgggagagtt cccgcacttc ggtctgttct tcagtgtccg cttcgaggat gagttgctcg
      181 ggttcgaaga atgggtcttc ctcgtcgagg gcggcgtggt agaggagccg gcgggccggg
      241 acccccagcg cagacgcgac tgcttccacc acgtcggtgg tggggttctt gcggcccttc
      301 tcgatgtctc cgaggtagct cttggagatg ccggactcgt cggcgagggc ttggagcgtg
      361 tactcgcgct ccatcctcag acggcggata gtcatgccta tgtagtctgc gagtcgtctc
      421 ggcttcatgt gctccctcgg tgccgcaccc gcgacgcagg cgcggtcgct gcgctgcagt
      481 ctatgaggaa gcacggcaga atgcaacggg ggagtgggtt gtgacgcgcg gcccgcacgt
      541 ctatcatggg gcgcacctgc cggcggtacc ccgcgcggct cgcacgaccg agaacctcga
      601 ctctggaatg cctcatctac atatgccccg aggccgcagg gtcgtctgac cgcggagccg
      661 acgatggagc aaggcagagc cggacggata ctctatctcg acccctggtc cggggtgtcg
      721 ggggacatgc ttctcggggc gcttctcgac ctcgataggg acgggcgtct cgaatgggct
      781 ctgcgcgagg cggtcggagc gctcggactg cctgaggccg gcgtggaggt cggcagaggc
      841 acgtcgggcg ggatcgcgtg cacgagggtg gacgtcgcgg tgtcgggcac ccctccgctg
      901 cgcagtctct ccgatctcca gaaccttctg gaggagtccg gcctcgcgga ggccgtcaag
      961 acccgcgcct ttcgagcact cgcccgactg gcgtcggtgg aggccggcat ccatggcgtg
     1021 accgtcgaac aggttcatct ccacgagctc ggtgcggtgg acacggtggt cgacgtggtg
     1081 ggctgtatcg cgcttgtgga cgctttgcac gtcgatgagg tggtgcacgg gcccgtcccg
     1141 ttggggacag gcagcgtcgt cacggcccac ggccgcctgc gcgttcccgc gccggcgacc
     1201 ctggctctgc tccaaggggt gccgatcttc gccggcccgg aggagtgcga ggtgaccacg
     1261 cccacggggg ccctgcttct gcgggaactg gccggacggt gtggcgcgat gcctgcgatg
     1321 atcgtggagg ccgtggggta cggcggaggg gcccgcacac tggaacatgg agccaacgtg
     1381 ctgagggctg tgatcgggcg ggcggcgtcg gccgagccgt ccgcctcggc ggcgacggca
     1441 tcctcgggcg agccggcgcg cgggcgccgg gcgtcagagg caggcttgcc ggagttcgtc
     1501 gtggaactgg aggcggtcat cgacgacgcc tcgccggaga tactggccta cctgcaccag
     1561 gcgctgctgt ctgcgggggc gctggacgcg tggtggacgc ccgcgttcat gaagaagggc
     1621 cgcccgggct tcgagctgac cgtcctgtgc cggcccgtgg acgaggttgc gcttgtggag
     1681 acgatcttcc gcgagtcgac gacgttcggc gtgaggcgaa gcgagcgctc ccggcacgtg
     1741 ctcgatcgcg aatgggtcca ggtggacgtg gatggcgaga cggtgcgggt gaaggtggga
     1801 cgcctgaacg gacgtgcggt gactgtggct cccgagttcg aggacgcggc gcgcgcggcg
     1861 gccggactcg gcactcccct ggcggacgtg atggcgacgg ccgcggcgtc cgcgcgcgcg
     1921 attctcgggg gctgaccccg cccgtcgcag ctacccgggc atcgctacag ctacccaagc
     1981 catcactgcg gctgtgacca tcgccgcggc gtcgggcggc tcacgccggg cccaccgtcg
     2041 cctcgaccga cgcctgcgcg aggggtgtcg gctctacggt gacgccgcgc tcgatgttcg
     2101 acgttcacat agttccgatg cgggccgaac ggtacgaatg ggccggtgaa cggcgcgctg
     2161 gagggttgcg cggcactctc gctattgatt tagagccagt cgttactata atggtccctc
     2221 gtacggttga ctcccccaac cacaggagga tcaggaggcg tgaagagcga catccagatc
     2281 gcacaggaag ctaccctcaa gcccatcatt gaggtagcgc gagacctcgg tcttggccag
     2341 gacgacatcg atctgtacgg caagtacaag gccaagatcc atctcgacgt cttggaccgg
     2401 cttgccgaca agccgaacgg caaactgatc ctctgcaccg cgatcacccc gacaccggcg
     2461 ggagagggca agaccaccac gaacgtaggt ctgtctcagg ggctggccaa gatcggcaag
     2521 agcgtcgtga cgacgcttcg tgagccctcg ttgggcccct cgttcggcat caagggcggc
     2581 gccgcgggcg gcggctacgc gcaggtggtc ccgatggagg acatcaacct ccacttcacc
     2641 ggcgacctgc acgccatcac gacggcccac aacctttgtg ccgccgttct cgacaaccac
     2701 ctgttccagg gcaacgacct cgacatcgat atcgagaaca tcacctggcc gcgcgtggtc
     2761 gacctgaacg accgtgctct gcggaacgtc accgtgggcc aagagggcga gggtgtcgag
     2821 cgcaagtccc gcttcgacat caccgtggcc tccgaggtca tggccatcct gtgtttggcc
     2881 accgacctcg aggatctgaa ggcgcggctg gaccgcgtca tcatcgccta caccaagtcg
     2941 ggcacaccgg tgaccgccaa ggacatcaag gtctcgggct cgatggccgt cctattgaag
     3001 gacgccctca tgcccaacct ggtgcagact cttgagaaca ccccggccat catccacggg
     3061 ggtcccttcg cgaacatcgc tcacggctgc aactccgtgc aggccacgaa gctcggcctg
     3121 aagctggccg actactgcgt gaccgaagct ggtttcgccg ccgacctcgg cgccgagaag
     3181 ttcttcgaca tcaagtgccg ctatgcggat ctgactcccg acgcggtagt gcttgtcgca
     3241 accgtacgcg cgctcaagat gcacggcggc gtggcgttcg cggacctgaa gactgagaac
     3301 ttggccgctc tggagaaggg actcgagaat cttcagaagc atgtggagaa catgcaactc
     3361 tacggcgtgc ccgtggtcgt tgccatcaac cgcttccccg acgacagcga tggcgaactg
     3421 aagctcctgg acgagaaggt caaggccatg ggctccaagg tggttctctc cgaggtgtgg
     3481 gccaagggtg gcgacggcgg cgtcgagttg gccgaagaga tcgtgcgcat gtgcgaggac
     3541 gagaagcccg acttcaagtt cctctattct ctggatctca gcatcaagga gaagatcgag
     3601 gcgatcgcga ccaagatcta cggcgccgac ggcgttgact acgcccccga ggtggaagag
     3661 gccctcgaac ttatcaccag gaacggctat tcgggtctgc cgatctgcat ggcgaagacc
     3721 caagcctccc tgtccgacga caagaccgtc gtaggccgtc ccagagggtg gaggctcacg
     3781 gtccgtgagg ctcggctgtc ggccggcgcg ggcttcatcg tgcctgtctg tggtaaaatg
     3841 atgaccatgc cgggccttcc caagaggccc gcggcggaga gcatcgatct cgtcgacggc
     3901 aagatcgtcg gtctcttcta ggagtcgcct catcccacgc tttagtcgca tggatagcgc
     3961 atgactgaac gctatacgct caccttccaa ccgggtgatg tagtggtcgc cgatgtgccg
     4021 cgcggagaat ccgttctccg cgcggccatg caggcggacc tccacataaa cgcctcgtgt
     4081 ggagggtcgg gctcatgcgg caagtgccgc atccagatca tcgccggcga ggtcgagagc
     4141 gagcgttcct ccaagctcga tgacgacgaa tgggccgagg gcatccgcct cgcctgccgc
     4201 acgcgagtgc tgtccgacgt cgagatcttc atccccgaga cgtcgaggat gggcaacgcg
     4261 gctgtgctgg agcgagaact ccgcggggag cgcaaaggga ggcttctgaa tcccgccgag
     4321 ctggagtcgc tcgtcaccgg gtggactctc gacccggtgg tcaagaagct ctgcatgcag
     4381 atgactccgc cctcggaggg cgataatctc aacgatctgg cccgattcaa gaaggcgctc
     4441 aaagacaact tcggcctggc caaggtgtcg gtggactccg tctggatcag gaacatgccc
     4501 cacgttctgc gcgagcacga ctggcaggtc acggccacgg tggtcgacac gtcgtacggc
     4561 tacaaggtca tcgagttgga gtcggggtct tattgcgacc gcgacttctc agtggtgatg
     4621 gacatcggca ccaccacggt gtgggggcaa ctcctcgatc tgaaccaggg cacgacgctg
     4681 gcacaggcct cccgctacaa caagcagatc agctacggcg acgacgtcat cagtcgcatc
     4741 gtcttctctc agaagccgaa cggtagggag actctgcagc aggccatcgt cgacacgatc
     4801 aacgagatcg tcgatgagct tgtgcagaag agcggcgtgg agcgcgagca gatcacgcac
     4861 atggtcgccg cggggaacac caccatgacc tgcatgttgg tgggtatcga cccgaagtac
     4921 ctgcgcatgg ctccatacgt tccgcccgcc gatttcattc cgccggtgcg cgccgtgaac
     4981 ttgggtgcgg gcatcaaggt gggccgtcac gttcacgtgc acaccttccc aagtgtggcc
     5041 agctacatcg gcggcgacat cgtctcgggc atcgtgggct ccggcatctt cgattcggag
     5101 cagatcaccc tctacatcga catcgggacc aacggcgaag tggtcatcgg caaccgcgag
     5161 tggctcatcg ccacatcctg ttctgccggc cctgcgtttg agggtggagg catcaagcac
     5221 gggatgcggg ccaccgacgc cgctatcgag acggtgagga tcgatggcgt tacctacgag
     5281 ccaaccctgc tcaccatcgg cgacaccaag cccatcggca tctgcggttc gggcatcatc
     5341 gacgccgtgg cggagcttct ccgagccggg gtgctctctc agaacggcaa gttcgacatg
     5401 tcgctgccga gccccaggct gcgtgagacc gagggcgcca acgagttcgt gctcgtggag
     5461 gccgagttct cgggcaccgg cgacgacatc gtcatcaccg aagccgacgt cgacaaccta
     5521 gtgcgcgcca aggccgccat cttcgcaggc atcatgacgc tgctcgagag cgtggcgctc
     5581 gattgggccg acctggaaca gatctacgtg gcgggcggct tcggcaagca tctgcgcatc
     5641 gagcaggcgc aggccatcgg cctgttcccc gaggtggaca tggacaagtt caccttcgtg
     5701 ggcaacggtt cgctgttggg tgcgcgcttg gtgtctttct cgaaggacat gctcaaggct
     5761 gccgagcagg tggcccgcat gatgaccaac gtggatatgt ccgacaacca gatgttcatg
     5821 gatcgctacg tggcggccat gttcctcccg cacaccgaca tgacgtactt ccccgagatg
     5881 gaagcgactc ttgccgcgcg ggcagggagg ggtcgctgat ggctgcgacc aagacggtgg
     5941 ccgtggccgg caagggcggc accggcaaga ccaccctggc gggcctgatg atccgggaga
     6001 tggtcgcccg cggcatctcg ccggtactgg cggtcgatgc cgatcccaac gcgaacctcg
     6061 gcgagatcct cgggaccaag gcggcggtca cggtgggcgg cctcagggag caggctttcg
     6121 tgggcgctcg tgagatccca acggggtggt ccaagcagag ctggatcgag tacaagatgc
     6181 acgaggcgat cgaagaggcc cacggctacg acctcctggt gatgggtcgc ccggaaggcc
     6241 ccggctgtta ctgctatgcc aacaaccttc tgcgcgagta catcaagacg ctcgcgaacg
     6301 agtacgcgtg ggtgatcatg gacaacgagg gcggcctgga gcacctcagt aggcatacca
     6361 ccagggatgt cgacgttatg ttcatagcca ccgacgcctc cgtgcgcggc gcccgcacgg
     6421 tcgagaggat ctcggagttg gcggacgaga tgggcttggt cattgcgcaa cgttacgtgg
     6481 tgatcagccg agctcccgag gggctgccgg tagaacggct catggagcac acttcggtgc
     6541 cgctcggcgg cgtcataccc gacgacccgg ctctgttcga gaacgatctg gccggcggta
     6601 gcgtgttcga cttgcccgat gatgcagtca gcctgggtgc ggtgcgcggg ttgatggatc
     6661 ggtatctcga catctgacgg gccgctggag agcggccctt gttgtgcgtg gggctgagag
     6721 gatcgttcag acgaaggagt gaagggaggc gggctggtgg caaagggcta catcgatggt
     6781 agcatccgcg agttcctcga caagctgggg acatcgagcc cggagccggg cggcggtagc
     6841 gtggcggccc tcgcggcggc tctaggcgcg ggtctggtga gcatggtcgc gagtctgacg
     6901 cttggcaaag agaagtactc cggcgtgcag gacgacatca aggtattgtt ggggcggtcg
     6961 gaggccgtga gggaagccct gcaggacttg gtgcagcagg acacggtggt atatggggcg
     7021 gtctcgacgg ccatgaagct cccgcgggat acggaggagc agagggccgc tcgtgaccga
     7081 ctgatgcagg ctgcgctggt cgaggccgcc aaggtgcccc tggcgatagc cgagcaggcg
     7141 ctggccgtgg cccagttgag cctgacggcg ggcgagatcg gcaacgtgaa cgcggtgagc
     7201 gacgcgggtg tggcggtgct tctggccgac gcggccgcgc agagcgcggc gctgaacgtg
     7261 aagatcaaca tcggatggat ccaggaccgg gcgttcaacg agggcagctg gtcacgggtg
     7321 caggagatcc tgagcgagac ggccgtgctg cgcgcgtccg tcatggggtt gacctacgag
     7381 aagctgggtt gaatcctgcc gggcgctgac gcggcccgtg gctcgacaac tacgaactgg
     7441 aggatagcgg atgggagcga aggtaataga cggaaaggct atcgcggctg agatccgtga
     7501 agagttggtg cgcgagctcg aggagctcaa gtcgcagggc atcgagcccg gcatcgccac
     7561 tcttctcgtg ggcgacaact tcggggcgaa gatgtatcgc ggagccgtcg agaagttctg
     7621 cgccgagatg ggcttcgcct atcgcgagga gacccactcc gccgacatca gcgaggaaga
     7681 agtcatccga atcgtggagt ccttgaacgc cgacccctcg gtgtctggga tactcccgct
     7741 gcggcccttc ccggagcaca tctccgattc cgcggtgatc aactcgatca gcgtgaacaa
     7801 ggacatcgac tgctttcatc ccttcaacat gggcaagctg gccgtgggtg agccgacctt
     7861 cccgccggcc actccctcag cgtgcatcga gctgttggag cggtatatga agagcgaagg
     7921 catcgacccc aaggacggtt tcgaaggggc cgagatctgc gtggtcggac actccaacat
     7981 cgtgggcaag ccggttggct tcctggccct caaccggaac gcctccacca gcgtcgtgca
     8041 cgtgttcacc tccatgaagg gcaacttgga gaagcacacc accgcggccg actacctcat
     8101 cgtggctgcc ggattcccca accttatcaa tgccgcgcag gtcaaagagg gcgcggttgt
     8161 catagatgtg ggcatcaacc gggtcatcgt ctgcccggtc tgcggcgcct acaaccggtc
     8221 caagaaggac gcgtgcaaga agtgtgcggc cgacttggcc gaagcagaga cgaggaccgt
     8281 cggtgacgtc aacttcgatg aggtcgtcga gaaggcgaag gccatcaccc ccgtcccggg
     8341 cggcgtgggg tcggtcacca acatgatgct ggcgaaaaac acactgcgcg ccgcgcggct
     8401 caccgccggc gcctgaggtt ccgcggaatg ggctgcggga ccgatcgaag aaaggagtga
     8461 tggacttggc ttttcaagct cctaacgagt cgtacaaggg agccatccgc gaggctgaca
     8521 tcgcgggagt gaccgtcggc ggagagacgg ccttcccgtt ccacgcgttc gagggggaga
     8581 tgcccaacac gccgaagctg gccttcgagg tgtgggacat cgcccccgac gattggtcgt
     8641 ctacgcttaa cgacgtgtac ggtgacgtgt tcaccgaccc cgtcgcgtgg gcgaagaagg
     8701 cggtcgacga attcggcgcc gacatgattt acctgcgtct tcgcagcacc gaccccaacg
     8761 gcgccgcgcg cgacgcggcc gaggccgccg cagcagcgaa ggccgtcgtc gaaggcgtca
     8821 gcgtacccgt gatcgtcgtt ggttccggcg atgccgagcg ggatcccgag gtattgaaga
     8881 ccgtggcggc ggcgctcgct gatcacaagg tggtcatcgg gaacgcggag gaagacaact
     8941 accgttctgt cggggccgcc gccatgggct atcagcacgc ggtagtggcg ttcagcccga
     9001 tggacgtgaa cctggcgaag cagttgaacg tgctgctggc tcagctgggt gtcgatgacg
     9061 gggcgatcct tatggatccc accacgggcg ctcttggcta tggtctcgag tatgcctaca
     9121 cggtcttcga gcgcgaccgc ctggcggcgt tggcgcagaa cgacccgaag atgcagatgc
     9181 ccattattgc cacagtcggt caggaggcct ggaaggccaa agagaccaaa gtgggcgaga
     9241 acgaactgcc cggtacgggc gacatggtca cccgtgggct gatgtgggag tcgctcacca
     9301 gcgtcgcact cttgctggct ggggcgaaca tcgtcgtcct acgccatccc gagtctgccg
     9361 ctcttatccg caaggctgtc gccagcctca agggcgagta gtagaaagga gccatcgacg
     9421 atcatgactg acgagaagaa gaaaaggggc ggggactacg cgaagtccat cgaccccgcg
     9481 gctctggaga tgctccagat tgccgatgag aagggcatct ccacgatgtt ctctcgggtc
     9541 gacgaagtgt cggcctgtcc tatcggagct gaaggcgcct gctgcaagat ctgcttcatg
     9601 ggtccctgcc gcttcgtcgg caaggacaaa gaagagaagc acggggtgtg tggagccagc
     9661 cttgccacag tcgccgcgcg gaactacatc cgctcgtgtg ccggcggggc gtctgctcac
     9721 gccgaccacg gccgtaccat cgccgagacc ctcctcgcgg tgggcaaggg cgaagccgcc
     9781 gactatcggg tgaaagaccc gattaagctg cgcacggttg ccggctactt cgacatcgat
     9841 gtcgagggta aggacgacaa ccagttggcc gtggaggtcg caacggcggc cctcaacgac
     9901 tggagcaagc acaacgagta ccagacttac gtcaagcgcg ctcccaagaa gcgccaggaa
     9961 ctgtgggcga agctgggagt gacgccacgc gggttcgatc gcgaggtggt cgattccatg
    10021 caccgcaccc acgcgggcgg cgatcaggac gcgccgcaca tcatggatca agctattcgc
    10081 atgtgtctcg ccagcggttg gggcggctcc atgcttgcca ccgacctctc ggatatcctg
    10141 tggggtactc cgaaacccgt gacgagcgaa gccaacctcg gcgtgctcaa tatcgaggac
    10201 gtcaacatca tcatccacgg tcacgagcct gtgctttcgg agatgatcgt ggacgcttcg
    10261 caagacccgg agatgatcga gtacgccaag agcaaaggcg cggcgggcat caccctggcc
    10321 ggcatctgct gcacggcgaa cgagatctgg atgcgccgcg gcgtcccgcc ggcgggcaac
    10381 tccctccacc aagaactggc actcctcacg ggcgccgtgg agctcatggt caccgacatc
    10441 cagtgtgtgt tccagggcat agtgggcgtg gcgaagaact tccacacgca cgtggtgacc
    10501 acctcgcaga aggctcacat cgagggcgcc atgcgcgtcg agttccacga ggatcgcgcg
    10561 ctcgagatcg ccaaggacat cgtgcgcatg gcgatcgaca acttccagaa ccgcgacgcg
    10621 gccaaggtgc acatcccgaa catcaagagc cccgtcgtgg ccggattctc ccacgagtac
    10681 atccggtaca tgctgggcgg caagttccgc gggagcttcc agccactcaa cgacaacatc
    10741 atcaacggca agatccaagg cgccgtggcg atcgtgggct gtaacaaccc gcgctccacc
    10801 caagacgccg gcatcatcaa cctggtcaag aacttcatcg agaacgacgt tctcgtgctc
    10861 gtgaccggtt gcgcggcgca cgcggccggc aagcacggct acctgacgcc tgagatgttc
    10921 gccaacgccg gccccggact ctccgaagtg tgcgagacca ccgggatgcc gcctgtcctc
    10981 cacgtgggca gttgcgtcga caacagccgc atcctcacga tcttggcgga tatcgtcgag
    11041 acgggtgggt tgggcgacga catcgaccag atcccagccg tgggcatctc gcctgagtac
    11101 tactgcgaga aggctttgga gatcgccacc tactgtgtgg gcagcggagc gtacgtcatc
    11161 ttcggcggcg tagagtctcc ggtgcgtgcc tccggggtgg tcaccgacat ccacacctcc
    11221 gggtgggaga agcagttcgg tggcaagctg gagttcatct ccgactggga ggggatcttc
    11281 gaggccgcta tggaccacat ccgcaagaag cgcgaagccc tcggcatcca cgaggagcat
    11341 gaacgcgtgc tgttcgacat ggccgatcgg cgggggtagc gtagatgacc actatgattc
    11401 agaaaagaaa acctgaaggc atccgcgaga tcgtcgcggg tggagtggcc cgcgggtcgc
    11461 gcgcctactt cgatctggcg aagagccgct tcgaagaggc ggcggcgggc atggacaccg
    11521 ggcagaagcg ggagttcttc ccgaataccg gctacttcct gcctgtgatc tacgcgatca
    11581 ccggccacaa ggttgagtgc ctcgacgaca tgcgttgggt gctcgacgag gccgaacgtc
    11641 tcttgccgcc ggtgccctcg aacaacctgt ggctgccgta tctcggtgag accctcgacg
    11701 gtggcatggc ctcgctgtgg ttggccgaga tcatcgaggc actgaagtac gtcggcgccg
    11761 gcccggcccc ggtcgacggc atctggctgg gggctgcgga cgacgtcatc atgcgcgctc
    11821 gcggtgtgga gttcgtagac ggttccgcgc cgggttttgc ggcggtgacg ggagcagccg
    11881 tcgatgtcga gacggccgtg gcactggccc gtgagatgca gcagaagtcg ctgtacgtct
    11941 tcatggccgg tgagtcccgc ggcaccacct tcgccgagca gttggcggag ggcggattgg
    12001 atatggcttg ggagacgcgc ctggtgccgt tcggacccga gatctacagc cacatctact
    12061 cggtcggttt cgccactcgg gctgctatgg ccttcggcgg cgtcaagccg ggcgacttca
    12121 agaacatcct caagtacaac aaggatcgcg tcttcgcctt cgtaatggcg ctcggcgagg
    12181 tcgacgatct gaagtacgct gcggcagcgg gtgccatcaa ctacgggttc ccgacgatcg
    12241 ccgagaccga tatcccggag atcctgccga cgggtgtgac cacctatgag cacgtggtct
    12301 ccaacattcc caccagcgag atcgtcgcca agtcgatcga ggtgcgcggg ctgaagatcg
    12361 tgatcaccga agtgccggtg cccgtggctt acggccccgc cttcgaggga gagcgcatcc
    12421 gcaaggagga tctctacgtg gagctcgccg ggccgaaggc tccgggcgcc gagttcaccg
    12481 tgatcgccga caacgtggaa gacggcaagg tgaccctggt cggtcccgat gtggaccaga
    12541 tcgaagtggg caccaccatc ccctacgcgg tgctcatcga agtctctgga cgcaagatgc
    12601 agaaggactt cgagccgatc ctggaacgtc agcttcacca cttcctgaac gaagccgaag
    12661 gcttcctgca tgtgggccag agggacatca tctgggagcg catcagcaag aaggctgtcg
    12721 ctgccggctt caagttcgag cacgtgggca agatcatcca cgcccgctat cacgcggact
    12781 tcggcggcat catggacaag gtgcagatca ccatctacac gcaggaacca gaggtgtcgg
    12841 agctggtggc ccgtgctcag agcgcatacc acgagcgcga cgaacgcatc ggcgcactcg
    12901 tggatgagtc ggtggagacg ttctacagct gttctctctg ccagagcttc gctcccgacc
    12961 acatctgcgt gatcaccccg gagcgcccgg gcctctgcgg cgcttacaac tggctcgacg
    13021 gcaaggccgc cttcgagatc agccccaccg ggccgaacca gcctatcgcc aagggcgtgg
    13081 ctgtggatga ggtcaagggt cagtgggtcg gggtcaacga gttcgtgtac caagcctcgc
    13141 acaacgccgt ggagcgcttc aacgcgtact ccatgatgga agacccgatg acttcgtgcg
    13201 gctgcttcga agccatcagc gtgcttctgc cgatgtgcaa cggcgtcatg gcggtcgacc
    13261 gtgaccacac gggtatgact ccctgcggga tgaagttctc aaccctcgcc ggttcggtgg
    13321 gcggcggtgc gcagaccccc gggttcgtgg gccactccaa gttctacatg ggctcctcga
    13381 agtacatcag cggcgacggt ggtgtggctc gggtggtctg gatgcccaag aagctgaaag
    13441 aggaactcaa ggacgccatt acggccagtg ccgacaaggc gggctatccc ggcctctacg
    13501 agaagatcgc gaccgaggag aacggcaccg aggaggagga ggtcatgacc tacctcgccg
    13561 aggtcggaca cccggcgctg gaaatggacc cgatgttcta ggagagggat gacgaccggg
    13621 cgcactatgg cgcccggtcc accccccctg aacgttgggg ggagcgtcca tgactagagg
    13681 agtgaaagga gtgacacgtt ggctctgagc gggcttcaga tattcaaact gctcccgaag
    13741 accaactgca aggactgtgg tttccccacc tgtatggcgt tcgccatgca gctggcatcg
    13801 gggaaagccg agctctcgca gtgcccccac gtctccgcgg aagcggcggc cgagttgggc
    13861 gaggccgccg cgcctccggt gcgcaaggtg acgatcggca ccggggactt cgcggtcgtt
    13921 gtgggcgaag agacggtcct gtaccgtcat gagaagcgtt tcgagcatcc gcctggaatc
    13981 gccatactgg tggacgactc ggtcgatgac gctgagttcc aggttcgtct ggacaccttc
    14041 aaagggcagg tcttcgagcg cgtcggccaa ctgctccgcg cgaagttgtt cgccatcgag
    14101 tcggctgacg ccggcaggtt ggcggcgcgg gtgaagaagg cgtcggacga gaccggtgcc
    14161 ggcatcgtcg tcatgagtga cgacccggcg gccctcaagg ctgccgccga acccatcaaa
    14221 gaccggaagc cgctcctgta tgcggccacg accgccactt tcgacgcggt ctcggctgct
    14281 gcgaaggagt tgggcgtgcc cttcgccatc aaggcggcga cgttggacga gttggccgag
    14341 atgggcgaga agtgtgtcgc ggcggacctc aaagaggtcg ttctcgatcc gatgtcggcc
    14401 aatctgcagg acgccctgcg cgatcagacc tacatcaggc gggcggcgat caagaagaag
    14461 ttccgtgctc tgggcttccc cacgatcgcc ttcccgtgca ggatgaccga cgaaccgctg
    14521 ttgaagaccg tgtacgcagg ggtcttcgta gccaagtatg ccgggatcat tgttatcgat
    14581 acggtcgacc cggcccagat catgccgctg ctgatcttgg cccagaacat ctacaccgac
    14641 ccgcaacggc cgatgcagat ggaccaaggg atctacccga tcaacgatcc gtcacccgac
    14701 agcccgttgc tgattaccac taacttctcg ctcacctact tcaccctcgc aggcgaggtg
    14761 gaggcgagca aggtgcccac gtggctcttg gtgatggaca cggaaggtca gtcggtgctc
    14821 acgtcgtggg ccgccggcaa gttcgtggcc gacgccatcg ccgtgttcgt gaagaagagc
    14881 ggcatcgctg agaaggtcaa ccatcgcaag atcgtcatcc cgggatacgt cgcgcagatc
    14941 agcggcgaac tggaagagga actgggcgac tgggaagtcg tcgtgggcgt gcgcgaagcc
    15001 gccgacgttc cgcgctatct gcgcgggctg tagtccgaca tcgaaaggtg ctcggccggt
    15061 ccgcgtggac cggccgggac gtagtgggag gcgggcggcc gtcggggccg cccgcccggg
    15121 aacggaggtc gagcagttga tcatcagcac gcagaagccc ttcgaggaga tcctcgagag
    15181 tctcaagggc gagaactcgg tctacatcgt tggttgcgga gaatgcgcca cgatgacgca
    15241 gagcggcggc gagcctgaag tcgccgacat gaaggcccgg ctggaggaag cgggcaagaa
    15301 ggtcgtagcc accgatgtgg ctcacgccgc ctgtcacgaa cttgatatga agcgcatttt
    15361 ccgtcagaac aaggaagctg ccacggctgc ggatgccttc ctggtcctcg cctgcggcgc
    15421 gggcgtccag tccgtgcgcg aaggcacgac gaagcacgtg acccccgggg ctaactcact
    15481 cttcgtgggc aacgtgaagc gccagatggc cttcgaggag aagtgctcgt tgtgcgggga
    15541 gtgcgtcctc gacgagtacg gcgccatctg ccccgtcacc cgctgtgcca agggcatcct
    15601 gaacggcccc tgcggcggca ccaacaacgg caagtgcgaa gtcgactcca acaaggactg
    15661 tgcctgggtc ctcatctaca accagatgaa gagcgaggac aaactcgcat cgttcatgac
    15721 catccacggt cccaagcgct ggaacgccat cgagcgtccg ggtcggctcg atgccagggc
    15781 cgctggcgaa gagaaagggg ctgcgtgatc atgagcaagt atcgcgaagc ttgtgagagc
    15841 ggcaggttcg tggtctccgg agagatcggc ccgccgaagg gcacgtccgt cgacaccatg
    15901 ctgcaccaca tcgagttgtt gaaggacctc gtcgacggtc tgaatgtcac cgacaaccag
    15961 agcgctgtca tgcgcctggg ctccatggcg gtgtcgcacg agatcgtcag tcgcggcggc
    16021 gacgccatct accagatgac ctgtcgcgat cgcaaccgcc tggctctgca gaacgacctt
    16081 ctctccgcgc acttcctcgg tctgcgcaac gtgctggctc tcaccggcga ccacgtcagc
    16141 gtcggggatc acaaggacgc caaggaagtc tacgacttcg agtcggtgca gctgatccag
    16201 tgcatcaagc gcatgaacga gggcttcgac tgggcaggca acgagttgga aggcggcact
    16261 gacttctaca tcggcgctgt ggtcaccccc gagtccgatc ccatcgagcc tcagaagctc
    16321 aagttcgaga agaagatcga ggcgggggcc cagttcttcc agacccaggc cgtctacgac
    16381 atggacaact tccggcgctt catggagtac gcgcggggag tcaccgaggg caaggacgtc
    16441 aagatgatgg ccgggctggt agtgctcacc agcgtgggca tggccaagta catgaacaag
    16501 ttcgtccccg gcgtgttcgt gcccgacgat ctcctggagg agatggcttc ggtgcccaaa
    16561 gaagaagccg tgaagaaggg tatggagatc tgcgcgcggc atatccggtt ctgcatcgac
    16621 aacgatgtct gcgatggcgt gcacgtcatg gccatcggca aagaggagat cgttcccgag
    16681 atcctccaga tggcgggtct gctgaagtag tcaagggtga cgcacgggga cgcggcagac
    16741 gccgcgcccc cgaccgatcg aggtggaggg agaccgacag tggcggaagc gacaaggatc
    16801 gtgatcatcg gcggcgggcc tggagggtat gtggcagcca tccgcgctgc ccagcgcggc
    16861 gccgacgtga ccgtggtcga gaaggacttg ctcggcggca cgtgtctgaa ccgggggtgc
    16921 attccgacga aggccctgct cgggagcatc gaggccctgc acaaggcacg ctccggcggg
    16981 gagttcgggt tcggtgtcac gggcgagata gtgccagatt tcacgcgcat gatggaacgc
    17041 aaaagcgaga tctcgaagac cctgcgcgac gggatccacg gccttttcaa gaagagcaag
    17101 gtcaaattgg tgcagggcga agggcgcatc gtcggccccg gcaaggtgga agccgcgacg
    17161 gccgagggcg tcgtgacgct cgaggccgac aagatcatca tagccaccgg ctccgagcct
    17221 gctcgactgc cgttcttcga tttcgaccag cccaccgtgc ttacgtctac gggagccctc
    17281 gaactgacga agatacccaa gaccctgctc atcatcgggg ccggtgtcat cgggtgcgag
    17341 ttcgcgaccg tgttctcgga attgggcacg aagatcacca tcgtcgagat gatgccgcag
    17401 atgctgccgc tcgaagacaa gcgcctggcg aagcagttcc agggaatctt caagaagaga
    17461 ggcatcgagg tcctgctcaa gacgaaggtg gatggcatcg cggagtacgc ggacgatcac
    17521 atcaccgcca acatcgaggg cggcgagcag atcacggccg agaagatgct ggtctcgatc
    17581 ggccgccagc cgaacagcgc cggcctcggt ctggagaccg tcggcgtcga gaccgacgcg
    17641 cgcggctaca tcgtggtgaa cgacaagctg gagacgaccg cacctgacgt gtacgccatc
    17701 ggcgacgtca atggcggcat cctcctggcg cacgtcgcct cctacgaggg cctcgtggcg
    17761 gtggagaact gccttggcgg caaccaggag cgagacctta aggtcgtgcc gtcgtgcatc
    17821 tacacgatgc ccgagatcgc cagcgtcggc ctcaccgaag accaagcgcg cgacgccggc
    17881 ttcgagccga tcagcggcac cttccggctt ggcgccgccg gcaaggcgct ggccatcggt
    17941 gaagctcaag gctacatcca gatcattgcc gatcaggcga ccgacaaggt gctcggcgcc
    18001 aacatgatgg gtccgcacgt aaccgacctg atccacgaga tcgcggtcgc tgtcaggaac
    18061 ggcctcaccg tgagggacat cggcgacacc atccatgctc accctacgat cggggaggcg
    18121 ttgatggaag ccgcgcacga cgtgcacgga gaatcggttc acgtagcgag ttgaagcgcc
    18181 tgtcgaggac ggaggcgccg gccgaggcga caacggtatg gagaacgacc gacaagtgac
    18241 gacgggcgta gctgagcatc ttgggcggaa gccggagtgg ctgaagaagc cgctcccgaa
    18301 ggctgccgcc gtgcatgggg tagaagggct gctccgtcga tcgagtcttc acacggtgtg
    18361 tgagagcgcg aagtgcccga acaagggtga gtgtttcgag cgaggcacgg ctaccttcct
    18421 catcatgggc aacgcgtgca ccagggactg ccgcttctgc agtgtcccaa gcgggcgtcc
    18481 ggagcttctc gacgcgagcg aaccgcaaca cgtggccgat gcggccgaga cgatggggtt
    18541 gagtcacgtg gtgatcacct cggtgacccg cgatgacctc gccgatggcg gggccgcaca
    18601 tttcgccgcg actatcgagg cggtgcgtgg gcgtctgccg ggcgcgacga tcgaggtgct
    18661 cgtgcccgat ttccaggagg acgccgcatc gcttgacgtc gtccttggtg ctcgtcccga
    18721 cgtgttcaac cacaacgtcg agacggtccc tcggctgtac gcccgagtgc gtccgcaggc
    18781 cgactaccgg cgctcgttgc gagtcctggc ctacgcggcc gccaagggga ccggagtggt
    18841 gaagaccggg atcatggtgg gacttgggga gagcgtcgaa gaggtcggga cttttctgca
    18901 agatgcggtg gccgccggtg tctcggtggt caccatcggc cagtacctgc gcccgagccg
    18961 agatcacctg ccggtgaccg agtacgtggc gcccgcagtg ttcgacgggt atcgccttgc
    19021 cggggaggcg ctggggctgg ttgtcgaggc ggcgccgttc gtccgcagtt cgtacaaggc
    19081 tgaagaaggt ttgcgccgcg tgagtggcga gagagtcaga gaatcaaggg aggatacgtg
    19141 ctagtaatcg ctgagaacat caacgtcatg gtcacgcgca tcggcaacgc catgaaggag
    19201 ggggacaagg ctcccatcca ggatctggcg cgcagggtgg tggaagccgg tgcggacgtc
    19261 atcgacatca acctcggccc cgcccgcaaa aacggcgaca agatgatgga gttcgtggtc
    19321 gagaccgtgg cggaggtcac gagactgccc ttctgtctcg acaccaccaa ccacgttgcg
    19381 atggaagccg gcatcgtgaa gtgtctggag ctcggcctgc cgcatcccat cgtgaactcc
    19441 ttctcggccc agcccgacaa gatggcgaac atccttccgc tcgcggcgaa gtacgactgc
    19501 gagatcatcg gactgaccat gggtgcggcc atccccatcg acgctaacga gcgcgtggcc
    19561 ctggcctacg aactggtgat ggccgccaac gagcagggta tctccaacga aaggatcttc
    19621 atcgacccca tcatccttcc cgtgggggta gacgtaggcc agcagcacgc cgtcgcggtg
    19681 caggaagtat tgaagatgct gcccgagatg ttcgatccca tggtgaagac cacgtgcggt
    19741 ctctccaaca tctccaacgg ggcgcccgac acaatgcgtc ccgcgatcaa caacgtgttc
    19801 gtggctatgc tggcggccgt cggcatgacc tccgccatcg tcgacgttct cgacaaagag
    19861 atgatgcgct cgatcaggct gatcaaagcc cttaccaacc agtccttgta
//
LOCUS       DASRMJ010000202         5162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_96446, whole genome shotgun sequence.
ACCESSION   DASRMJ010000202 DASRMJ010000000
VERSION     DASRMJ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96446"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1616)
                     /gene="mnmE"
                     /locus_tag="VFD74_05580"
     CDS             complement(63..1616)
                     /gene="mnmE"
                     /locus_tag="VFD74_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZK49055.1"
                     /translation="MNPIRPKPNTPDRNEPTTAASSAAALPQVRRDAAAAGIASPHAD
                     TIVAVSTAAGAGAIGIVRMSGPRAVELGDGVFRPAHGGTLGASAAGRVRYGHVIDPER
                     GVVVDEVLAVAMRAPSTYTREDVVEIHCHGGPAAQRAVLQLLMHSGARAAEPGEFTQR
                     AYLNGRIDLTQAESVAGIVQARTSSALRACVRQLSGGLSQRLREVRAGLVGCLAQLEA
                     GIDFTDEDIEELDREALALELEKARADLGILLDTAFLGRALENGVRTAIVGRPNVGKS
                     SLLNALLMRERAIVSDLPGTTRDTIEELIEVAGIPLHLIDTAGLRPSKDVLERLGIER
                     SHEAMREADLVLAILDLSAPIQPEDAEMVGRLDPRRTIIVGNKRDKVAAPPAAESGWP
                     EYLASMLDGVVEPDGGLTEHRQMLAVSALTGEGMDALRALIEQVVVGEGGIELDEPIL
                     ATERQRSLVEDSATAIDATLRAIRRGLAEELIAEDVRVAILALGRITGDELVPDLIDE
                     IFRRFCIGK"
     gene            complement(1969..2814)
                     /gene="jag"
                     /locus_tag="VFD74_05585"
     CDS             complement(1969..2814)
                     /gene="jag"
                     /locus_tag="VFD74_05585"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HZK49056.1"
                     /translation="MEAAVAQPGNGRDPSSRIRTASASGRSVEIAVARALAELDYAPG
                     ELPEDQYSVTVIGEPDEGFLGVGGSDAVVEVRLLFDDRRSGEGADTVPDDEDDLDGPT
                     GNVRPPDEEFDDDFEEGDAYDADNADAETPAAGSARLREFLGVVLQALGVEARVRIVE
                     EVDVLKAEVVAEDLGVFIGRRGQTIDAIEYLASLTLYPRPESRKRVEIDAAGYKERRR
                     QGIERVALHKAQEAARSGRPVELEAMTAAERKIVHVTLKDRRDVTTESRGREPNRAVV
                     ILPTL"
     gene            complement(2744..3505)
                     /locus_tag="VFD74_05590"
     CDS             complement(2744..3505)
                     /locus_tag="VFD74_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HZK49057.1"
                     /translation="MRSILDPIVQFLLAILTFVHDNLHLNWGWSIVVLTVIVRVVLIP
                     LTWKQFKSMRAMQTLQPQIKALQEKYKADRQVLNQKMMEFYKENSVNPFGSCLPLVLQ
                     MPVFFALFYMLQAESGAGGHFATDNQWLWMRAWESAPSWVAANITMFDLPLLILYVAS
                     QFVSSLQMASKDSAQRNMMLAMPIGIGVIMYLGKWPAGLFIYWFTSNLWSIGQQLIIK
                     KTVPVPEPAAVLSGGGSGSTGKRKRSKQQNPDGKR"
     gene            complement(3502..3771)
                     /gene="yidD"
                     /locus_tag="VFD74_05595"
     CDS             complement(3502..3771)
                     /gene="yidD"
                     /locus_tag="VFD74_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017600678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZK49058.1"
                     /translation="MLPIRGYQRLLSPALPRRCKYHPSCSQYAIDAVRAHGALRGSIL
                     ASWRVLRCNPFSDGGFDPVEGQRLFHTHAREACHGAGEDLEAAIR"
     gene            complement(3783..4187)
                     /gene="rnpA"
                     /locus_tag="VFD74_05600"
     CDS             complement(3783..4187)
                     /gene="rnpA"
                     /locus_tag="VFD74_05600"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZK49059.1"
                     /translation="MIRRRSRLSRSQDFDRVYRAGRSVANRYLVLYCFQRDEPARPAV
                     GERAAARVGFSVSKRLGSAVERNKVKRALREAYRLNEHRVTGDRDFVLIARVPLLDLL
                     EKSGLAVVEEKVIEVFQKASSMAPGEGRTSDS"
     gene            complement(4196..4330)
                     /gene="rpmH"
                     /locus_tag="VFD74_05605"
     CDS             complement(4196..4330)
                     /gene="rpmH"
                     /locus_tag="VFD74_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZK49060.1"
                     /translation="MKRTYQPNKRKRKKTHGFRKRMSTKAGRAVLKRRREKGRKRLSA
                     "
BASE COUNT          873 a         1775 c         1569 g          945 t
ORIGIN      
        1 gtccggcaaa gagaggcgcc agacaacttc gtggagtgga gtgcgctcac ctcgagtgcg
       61 ccttacttcc ctatgcagaa gcgtcggaat atctcatcta tgaggtcggg gacgagctcg
      121 tccccggtga tgcggcccaa agcaaggatc gccacgcgca cgtcttcagc aatcaactcc
      181 tcggcaaggc cgcggcggat agccctgaga gtcgcgtcga tggccgtcgc tgaatcttcc
      241 acgagggatc tctgccgctc cgtagccaag atgggctcgt ccagttcgat gcccccctct
      301 ccgaccacga cctgctcgat cagagctcgc agcgcatcca tcccctcgcc tgtcagcgcg
      361 gagaccgcca acatctggcg gtgttccgtc agcccgccgt ccggctcgac caccccatcc
      421 agcatagatg ccagatactc cggccagccg ctctccgcgg cagggggcgc ggcgaccttg
      481 tcccgtttgt tcccaacgat gatcgtccta cgcgggtcca gccttccgac catctcggcg
      541 tcctcaggtt ggatcggggc cgacaggtcc aggatcgcaa gaaccaggtc ggcttcgcgc
      601 atggcctcgt gggaacgctc gatccccagg cgctccagta cgtccttgct cggccgaagc
      661 cccgccgtgt cgatgagatg cagtgggatc ccagccactt cgatgagttc ctcgatcgtg
      721 tcgcgggtgg tcccggggag gtccgacacg atggcgcgtt cgcgcatgag tagcgcgttc
      781 aacagggaac tcttgcccac gttcggacga ccgacgatcg ccgtccgcac accgttctcc
      841 agtgcgcgcc ccaggaaggc ggtgtccagg aggatcccga gatccgctcg cgccttctcc
      901 aactcgagag caagtgcctc gcggtcgagt tcttcgatgt cttcgtcggt gaagtctatg
      961 ccggcttcga gttgggccag acaacccacc aacccggcgc ggacttcgcg caatcgctgc
     1021 gacagaccgc ccgacaactg tctcacgcaa gcccgcaacg cgctggacgt gcgggcctgc
     1081 acgatcccgg cgacgctctc ggcttgggtg aggtcgatac gaccgttgag atacgccctt
     1141 tgtgtgaact caccgggctc ggcggcgcgc gctcccgagt gcatcaacag ctggaggacc
     1201 gcccgctgag ccgccggacc gccgtgacag tggatctcta ccacgtcctc ccgggtgtac
     1261 gtggaggggg cccgcatggc gaccgcgagc acctcgtcca ctacgacgcc acgctcagga
     1321 tcgatcacgt gcccgtatcg cacccgcccg gctgcagacg cgcccaaggt cccgccgtga
     1381 gcgggtcgga acactccatc acccagctcg accgcgcgcg gcccgctcat ccgcacgatg
     1441 ccgatggctc cggctccggc ggccgtcgag acggcaacga tcgtgtccgc gtggggcgat
     1501 gcgatacccg ccgccgcggc atcccgccgg acttggggca gtgcggcagc gctcgacgcc
     1561 gcggtagtcg gctcgttgcg gtctggtgtg ttgggtttcg gtctgatggg gttcactcgc
     1621 atcacggttc tcactggtgc tcggcggcgc gcgccatacg gcaaaccggc agggggaccg
     1681 ccttcggccc cgcaggttgc gcgcgccggc gtgtaggagg gaccactgct cggggcggcg
     1741 gctgccccgc gccaagccgg ctgcctcgcg ccaagccgac tggacgacgc cacatttgcc
     1801 gatccgtgcg gtgtcagtcg ccccgcggca ctccgccagg tcgcccgccg cgccgcggcg
     1861 ctcagtcggg tcgcccgtcg ccccacaggg ctatgtcagg tcgcccgtcg cgccgccaca
     1921 gccggcccgc ccgcgcaaca cctcggctcg tccgctccag catccgtcct agagcgtggg
     1981 aaggatgacc accgcccggt tcggctcccg gcctcggctc tccgtggtca cgtcgcggcg
     2041 gtccttgagt gtgacgtgca ctatcttccg ctcggctgcg gtcatggctt ccaactctac
     2101 cggacgcccg cttcgcgccg cttcttgcgc tttgtggagc gctactcgtt cgatgccttg
     2161 gcggcggcgc tccttgtatc cggcggcgtc tatctcgact cgcttccggc tctccgggcg
     2221 cggatacaga gtgaggctgg ccagatactc tatggcatcg atggtctggc cgcgccgccc
     2281 gatgaacacg cccagatcct ctgcgacgac ctccgccttg agaacgtcga cctcttcgac
     2341 gatgcgcact cttgcttcca ccccgagagc ctgcaggacg acgcccaaga actcgcgtag
     2401 tcgcgccgaa cccgcggcgg gagtctcggc gtccgcgttg tcagcgtcgt aggcgtcgcc
     2461 ttcctcgaag tcgtcgtcga attcctcgtc gggcgggcgg acgttcccgg tgggaccgtc
     2521 gagatcgtct tcgtcgtcgg ggaccgtgtc agcaccttcc cctgaccgcc tgtcatcgaa
     2581 gaggagtcgc acctccacca cagcgtccga accgcccacc ccaaggaaac cctcgtcggg
     2641 ctctccgatc acagtgacgg agtactggtc ctccggcagc tccccgggcg cgtagtccaa
     2701 ctccgcgaga gctcgagcga ccgctatctc gacggagcgc ccgctagcgc ttgccgtccg
     2761 gattctgctg cttggatctc ttccgtttcc cggttgagcc actgccgcct ccactcaaaa
     2821 ccgctgccgg ttcgggcacc ggtaccgtct tcttgatgat gagctgctgg ccgatcgacc
     2881 agaggttgga cgtgaaccag tagatgaaca ggccggccgg ccacttgccc aggtacatga
     2941 tcacaccgat gccgatcggc atcgccagca tcatgtttcg ctgagccgag tccttcgagg
     3001 ccatctgcag ggaactgacg aactggctgg caacatagag gatcagaagc ggcagatcga
     3061 acatcgtgat gttggccgct acccacgaag gcgccgactc ccacgcacgc atccacagcc
     3121 actggttgtc ggtggcgaaa tgaccgccgg cgcccgattc cgcctgcaac atgtagaaca
     3181 gcgcgaagaa gacgggcatc tgcagtacca gcggcaggca ggacccgaac ggattgaccg
     3241 agttctcctt gtagaactcc atcatcttct ggttcaggac ctgacgatcc gccttgtact
     3301 tttcttgaag cgccttgatc tgcggttgca gggtctgcat cgcccgcatg ctcttgaatt
     3361 gcttccacgt gagcggaatc agcacgacgc gcacgatcac ggtcaggact acgatcgacc
     3421 aaccccagtt gaggtggagg ttgtcatgca cgaacgtgag gatcgccagc aggaactgga
     3481 cgatggggtc aagaatggat ctcatctgat agcggcctcc agatcctcgc ccgctccatg
     3541 gcaggcttct ctagcatggg tgtggaacag ccgctggccc tctaccgggt cgaacccacc
     3601 gtcgctgaac gggttgcagc gcaggacgcg ccacgacgcc aagatcgacc cccgcagggc
     3661 gccgtgggcc cggaccgcgt caatggcgta ctgggagcac gatgggtggt acttacacct
     3721 gcggggaagc gcgggcgaaa gcaagcgttg gtagcccctg atgggcagca cgatgatctc
     3781 gcttatgagt ccgatgttcg cccctctccg ggagccatcg acgaagcctt ctggaagact
     3841 tcgatcactt tctcttcgac aaccgccagc ccactcttct caagcaggtc gagcagcggg
     3901 acccgggcga tgaggacgaa gtcgcgatcc ccggtcacgc ggtgctcatt gagacgatat
     3961 gcttcgcgta gtgcgcgctt caccttgttg cgctccaccg cggatcccaa cctcttcgag
     4021 acagagaacc cgaccctcgc cgccgcccgc tcgccgaccg ccggcctcgc cggttcgtct
     4081 ctctggaaac agtagaggac caggtagcga ttcgcaacag acctccccgc tcggtacacg
     4141 cggtcgaagt cctgcgaccg agagagacga cttcgccggc ggatcacagg tcgccctagg
     4201 cagagaggcg cttccggcct ttctcacgac ggcgcttcag gaccgcgcgc cccgctttgg
     4261 tgctcatgcg cttgcgaaag ccgtgagtct tcttccgctt ccgcttgttc ggctggtacg
     4321 tccgtttcac aacgactcct gtctttctcc atgctcaagc agaccttcgt ctgcgatccc
     4381 tcaaagggcg ggaagtatac aagtagcagc gccgggcgtc aaacagcggc cgcgcccgtc
     4441 tcgcgtcccc tcacagcgtt cttgcggctg tgcagactgt ggacaaacca gcccgctccc
     4501 ttacgcgcag acctcgccgg cgtggtcgga tgtggaatac cgttcaccac gcacgcccag
     4561 ggcgtacgga tgccgacaga tatcgccgtt tctcttcttg gttcctttct ctatttccgc
     4621 aaactgggcg cttttccttc gaaagagtcg cgcatcgatc cctcttccgc gcctcgggca
     4681 atctcgaggc cgaccgaggt cgccaccggg ctcgcctaag tcggccagcg aagcagccga
     4741 ggcctcccga cgaccccccg gaacgagccg gccgtgaccg cttcctggaa tgcatatccc
     4801 cagaatcccc agggagaccg ggcgtagggc tccgatggtt tcacgtttcg caccattgtc
     4861 cacagatgtg catcgtgctg ttgataactc tgcgttctac ctgcaaacaa ggatttgtcg
     4921 agagaagaca cgagcgaaat agacctcgcg gacacccccg aatcaagggc taaccggggg
     4981 tcccgcccct tcgggcctgt ggaaactcca ctcttcaagc gctacaatga gcgctcgttt
     5041 ctggaaggct cgtgaggggt gcccggggcg ggccggggag tgtcttctga tcgcgccacc
     5101 ccgggctcgg cgggcgtgaa atgagggacg atcaggtggg agagttagac gccagtgggg
     5161 tg
//
LOCUS       DASRMJ010000203         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_236484, whole genome shotgun sequence.
ACCESSION   DASRMJ010000203 DASRMJ010000000
VERSION     DASRMJ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236484"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..496)
                     /locus_tag="VFD74_05610"
     CDS             complement(353..496)
                     /locus_tag="VFD74_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49061.1"
                     /translation="MSRGALGQPMSRVAPPLREVGWRHFLLALPGVVVSLVPVAALPA
                     AAA"
     gene            complement(642..929)
                     /locus_tag="VFD74_05615"
     CDS             complement(642..929)
                     /locus_tag="VFD74_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49062.1"
                     /translation="MGLIQRVLDEAGISTVSLTLVREMTALAKPSKALYVQHPFGLTL
                     GAVGDRITHEAILRDCLRYAEQTLPAGEIVELPYRWEVDDLRERQLRKQAH"
     gene            complement(948..1451)
                     /locus_tag="VFD74_05620"
     CDS             complement(948..1451)
                     /locus_tag="VFD74_05620"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HZK49063.1"
                     /translation="MSSGMAADFSVVEGDFLRSKFLPDFEWARFDRPTESVALARPIE
                     QARVALVVTAGAYLPACQEPFNTKTFLGDDSFRVIPGDARREDIGLCHPGYDTRRAAQ
                     DLDCVFPLALLRELQGEGAIGEVAPRHVSFMGYIPRTEHLITETAPEAARILADDGVD
                     LAILVPS"
     gene            complement(1483..2013)
                     /locus_tag="VFD74_05625"
     CDS             complement(1483..2013)
                     /locus_tag="VFD74_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HZK49064.1"
                     /translation="MPRIEPFSTEKVDEAVAATFAEIEGAFGMVPNLFRTYAYNPALL
                     EANWNKVKALMMGGVLDRKVKEAIAVLVSKDNSCAYCVAAHTMALRSIGVSEEEIAAL
                     EDDLQKAGFSSKEEALMQFARRANLAPRDITDVEFQAVRDLGATDAEIVEALGVMELF
                     TGFNKFLDTLQVDLDF"
     gene            complement(2070..>2427)
                     /locus_tag="VFD74_05630"
     CDS             complement(2070..>2427)
                     /locus_tag="VFD74_05630"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZK49065.1"
                     /translation="GKKLTAFLAAVLVAHREAGFVGGCIFGNTALEMGDTDAAYGRVV
                     GEVFDRWAARIEEIVADAQSAGEVRKDLSPDRVASHIVAALEGGIMLSRLKKDEEPLA
                     TSVECLVAFLQPPKSV"
BASE COUNT          451 a          804 c          771 g          401 t
ORIGIN      
        1 caggtgatca gcggatctcg ggtgggctct ctgagccaga ggagggacaa gcctggtctc
       61 ccgacctccc gacgggccaa gcgttccacg ccgacgccgc cacgaggaag gccgagcctg
      121 tgtaggcgaa tacgtccgag gccagggcga acctgcccac gagcaccaga gccgcggcca
      181 ctaccccgag caccaggggt ggatagcctc gccggcgcct ggcccgccaa ccgagggccg
      241 ccaaggccaa ccccagcagg agtccggtca gaggcaggag ataggcggtc ttcagtgcaa
      301 agcggagccc cagcgcacca gccagaccgg caactgccga caggcaggaa gatcaggcgg
      361 cggcagccgg cagggcggcc accggcacca gagagacgac gacacccggg agtgcgagca
      421 agaagtgccg ccaaccgact tcgcgaagag ggggcgcgac ccggctcatt ggttgtccaa
      481 gggcacctct actcatgagg ttctctccct aagatcctga gaaaaggacg gtcccccgcg
      541 ggagcctcgc gacagagcgc ggccagttcg atgagagcct cccgcgtggc gcgacgagtg
      601 gacccgaaat ccttggaccg tctgatgagg gagacgagca cctagtgcgc ctgcttgcgg
      661 agttggcgct cgcggaggtc gtcgacctcc cagcggtagg gcagctccac gatctcgccc
      721 gccggcagtg tctgttccgc gtagcgcagg cagtcccgca agatggcttc gtgggtgatc
      781 cgatcgccga cggcgcctag ggtcagcccg aacgggtgtt gcacgtagag ggccttcgag
      841 ggcttcgcaa gggcggtcat ctcccgcacc aaagtcaacg aaacggtgct gatgcccgct
      901 tcgtccagaa cccgctggat cagtcccacg gactggctgc agaccggtca cgagggcacg
      961 agtatggcca ggtcgacccc gtcgtcggcg aggatgcgcg cggcctcggg cgcggtctcc
     1021 gtgatcaggt gctcggtccg cgggatgtac cccatgaaag agacatgtcg tggggccacc
     1081 tcgcctatcg cgccctcccc ctgcagctcg cggagcaggg ccagggggaa cacgcagtcc
     1141 agatcttggg ctgcccgcct ggtgtcgtag cccggatggc acaggccgat atcctctcgg
     1201 cgggcatcac ccgggatcac gcggaaacta tcgtcaccca agaaggtctt tgtgttgaac
     1261 ggttcctgac acgcgggcag gtacgctccc gcagtgacga ccaacgctac ccgggcctgc
     1321 tcgatagggc gggccaaggc caccgattcc gtgggccggt cgaaccgggc ccactcgaaa
     1381 tcgggcagga acttgctgcg caggaaatca ccttccacga ccgagaaatc cgccgccatt
     1441 cccgaactca tgccgctcct ttcgcgcgaa gtcactcgtg gatcagaaat cgaggtccac
     1501 ctgaagcgta tcgaggaact tgttaaatcc ggtgaatagt tccatcacgc ccagggcttc
     1561 gacgatttcg gcgtcggtgg cgccgagatc ccggaccgcc tgaaactcca catccgtgat
     1621 gtcgcgtggg gccaggttcg cccgacgcgc gaattgcatg agcgcttcct ctttggagga
     1681 gaagccggcc ttctgcaagt cgtcctccag agcggcgatc tcttcttcgc tcactccgat
     1741 ggagcgcagg gccatcgtgt gggcagccac gcagtaggcg cagctgttgt cttttgagac
     1801 cagcaccgcg atagcttcct tgaccttccg gtcgaggacc ccgcccatca tgagtgcctt
     1861 caccttgttc cagttggcct cgaggagcgc aggattgtag gcgtaggtgc gaaacaggtt
     1921 gggcaccatc ccaaaagcgc cctcgatctc ggcaaatgta gcggcgacgg cttcgtcgac
     1981 cttctcggtg gaaaacggct cgatccgcgg catcacacac gactccttct ctggtgagac
     2041 gtcttcgacg cctcgtttct cggatggctt cacacgctct tcgggggctg gaggaaggcg
     2101 accaggcact ccacgctcgt cgcaagcggc tcctcatcct tcttgagccg agagagcatg
     2161 atgccgcctt ccagggcggc tactatgtgg ctggccacgc gatcgggtga gaggtctttc
     2221 cgcacctcgc cggcgctctg tgcgtccgca acgatttctt cgattcgcgc ggcccaacgg
     2281 tcgaagacct cccccacgac acgaccgtag gccgcgtcgg tgtcacccat ctcgagcgcg
     2341 gtgtttccga agatgcagcc ccccacgaaa ccggcttccc ggtgcgcaac gaggacggcc
     2401 gccaggaacg cggtcagctt cttcccc
//
LOCUS       DASRMJ010000204         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_160041, whole genome shotgun sequence.
ACCESSION   DASRMJ010000204 DASRMJ010000000
VERSION     DASRMJ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160041"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFD74_05635"
     CDS             complement(<1..594)
                     /locus_tag="VFD74_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZK49066.1"
                     /translation="MFERFTERARQVVVLAQEEARDLKHNYIGTEHLLLGLLREGDGV
                     AARVLSTLDVSLEDVRGEIMRIVGEGEHEAQGQIPFTPRAKKVLELALREALSLGHNY
                     IGTEHILLGLVRESEGVAARIMNDLDADADRIRQEVMRVLSGPSRRSQKPGVPAGQAK
                     KSSKVLDQFGRNLTKFAEEDKLDPVIGREMEIERVMQI"
     gene            complement(606..1628)
                     /locus_tag="VFD74_05640"
     CDS             complement(606..1628)
                     /locus_tag="VFD74_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49067.1"
                     /translation="MMADSVRLASGLTISRNEEVLPFPHAASEDMLCTFRARVESALS
                     RHGAPGEWEFFDMDAISSAQRAYLVERGVMTPGFAKGVGRSRAFGLFQGGRAALEING
                     TDHLRMLGSGPPDGLSDVWATLDHIDDTLESEIAYAFDERWGYLTARAADSGTGLRAF
                     LTLHAPGLMITGRLAAVAASLAGEGLALAPLWSGAGGVFQVFNRKALGTSEGRITEAV
                     TAATRDIVEQERTVRKGLLRENPVSVRDHIGRALGVAQQAWSVSVAEALTLVSSIQVG
                     VSMDLVEGPGIDAGATLSLMRRVQSAHVVVDGMGSGLGGLEDPRVDEVRAQLLRRIFA
                     GARVAQ"
     gene            complement(1628..2188)
                     /locus_tag="VFD74_05645"
     CDS             complement(1628..2188)
                     /locus_tag="VFD74_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HZK49068.1"
                     /translation="MFSEGSDWQAAASGGRTCESCGEEPATIHVLHFDGSAVTHTHLC
                     ASCAEEEAGHVDGAALVVALPSMLGTVASAMISAVSSEREHGFAPICAVCGTTLNDFR
                     ENGLFGCAACYQVFQSYLDEELEDRTAGEHLGKVPTRTPGGQHQRREVVRLQRMLSEL
                     VESERFEEAASVRDRLAELEGQVSAG"
     gene            complement(2418..3905)
                     /gene="lysS"
                     /locus_tag="VFD74_05650"
     CDS             complement(2418..3905)
                     /gene="lysS"
                     /locus_tag="VFD74_05650"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZK49069.1"
                     /translation="MPSTPHLSELERERRSKADRLSSAGVELFARRFYPRSSAHEVLS
                     THADLVAGESAEDAVYRVAGRVMARREHGKAAFLTLRDGWDDLQVYANVNTVGQESFD
                     LLTGLDLGDIIGVKGHAFCTRRGELSLFVSEWTLLTKSFHAPPEKWHGLQDKETRYRQ
                     RYADLLSNPEVGRQLTARGAIVGAMRRYLYERGFLEVETPILQPLPGGALARPFATHH
                     NALDVELYLRIAPELYLKRLLVGGYDKVFEIGKNFRNEGISPIHNPEFTMMELYWAYV
                     DYREIMELVEEMIPAVAQEVLGSTMITVDGREIDLRGPWPRRTVDELVRGAIGMGLAD
                     IRADTEAARSVLRVKGVDVEGRDTFSSLVDKTLKHLVWPSVIEPTFVIDHPLELSPLA
                     KVHPDDPSLVERFQPIIAGREVGNAFSELNDPRDQRRRFEAQALNKAAGDEEAMVLDE
                     DFLRALEYGMPPAGGLGIGIDRLAMLLLGAESIRDVILFPQLRPE"
     gene            complement(3954..4433)
                     /gene="greA"
                     /locus_tag="VFD74_05655"
     CDS             complement(3954..4433)
                     /gene="greA"
                     /locus_tag="VFD74_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZK49070.1"
                     /translation="MHHKEVVLTQEGLRHLREEIDRLSTTEREKVAERIREARDFGDI
                     SENSEYDDAKNDQAMLEHRIGQLEEKLRRARVIKDSEISTGKVSVGVTVTLKDLEYDE
                     ELVYQLVGSAEADPMKQKLSNESPMGQAIIGRVPGDVVTVPAPIGSLKYEILKIEVA"
     gene            complement(4675..>5085)
                     /locus_tag="VFD74_05660"
     CDS             complement(4675..>5085)
                     /locus_tag="VFD74_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HZK49071.1"
                     /translation="RADHAVTAAVAASVGVPVLASGDVDSAASAAAIVAATGASAVMI
                     ARGAERGPWLLADVLAGTDQGRRPPIEVVTELRALVSLAAADMGAHRAARWSRKIAGR
                     YLRGLGFAQEDLDRLRAAPDAAAVDAALAALIAG"
BASE COUNT          864 a         1731 c         1633 g          857 t
ORIGIN      
        1 gatctgcatg acccgctcga tctccatctc cctgccgatg acggggtcga gcttgtcttc
       61 ctcggcgaac ttggtgagat tgcggccgaa ctggtcgagc accttggacg acttcttggc
      121 ttggcccgcc ggcacacccg gcttctgcga ccggcggctg ggcccggaca gcacccgcat
      181 gacctcctgg cggatacgat cggcgtcggc gtcgagatcg ttcatgatgc gcgccgccac
      241 cccttcgctc tcgcgcacga gacccagaag gatgtgctcg gtgcctatgt agttgtggcc
      301 gagcgagagc gcctcgcgca aggccaactc cagcaccttc ttggcacgcg gtgtaaaggg
      361 tatctgcccc tgcgcctcat gctcgccctc gccgacgatg cgcattatct ccccgcgcac
      421 gtcttcaagg gacacatcca aggtgctcag cacccgggca gcgacaccgt caccctcacg
      481 gagcagtccc aggaggaggt gttccgtgcc gatgtagttg tgcttgagat cgcgcgcctc
      541 ctcttgggcc aataccacga cctgtcgagc gcgctccgtg aaccgttcga acatctctgc
      601 ctctcctact gcgcgacgcg cgccccggcg aagatccgcc ggagcagctg cgcgcgcact
      661 tcatcgacac gtggatcttc aagaccgccc agacctgagc ccatgccatc gacgacgacg
      721 tgggccgact ggacgcgacg catgagcgag agcgtggcgc cggcatcgat gccgggcccc
      781 tcgaccaggt ccatggagac gccgacctgg atagaagaca ccagggtaag cgcttcggcc
      841 accgagaccg accacgcctg ctgcgcgaca cccagcgccc tgccgatgtg atcgcgcacg
      901 ctcacgggat tctccctcaa cagccccttg cgcaccgtcc gctcctgttc gacgatgtcg
      961 cgggtggctg ccgtcaccgc ctcggttata cgcccctccg atgtgcccag ggccttccgg
     1021 ttgaagacct ggaagacccc gcccgccccg ctccacagcg gcgcgagcgc caatccttcg
     1081 cccgcgagcg aagcggcgac ggcggcgagt ctccccgtga tcatcaggcc cggcgcgtgc
     1141 agggtgagaa aggcccgaag gccggtgccc gagtctgcgg cgcgcgccgt gagatagccc
     1201 cagcgttcgt cgaacgcata cgcgatctca ctctcaagcg tgtcgtcgat gtggtcaagc
     1261 gtagcccaca cgtcggacag accgtcgggc gggcccgagc ccagcatccg caggtggtcg
     1321 gtaccgttga tctccagggc tgcccgtccc ccttgaaaga ggccgaaagc acgagagcgg
     1381 cccacgccct tggcgaaccc cggggtcatg acaccgcgct ccaccaggta ggctcgttgt
     1441 gccgaggaga tcgcgtccat gtcgaagaac tcccactcgc cgggcgctcc gtgcctggac
     1501 agcgcgcttt cgacgcgcgc ccggaaggtg cacagcatgt cctcgctcgc ggcgtgagga
     1561 aacgggagca cctcctcgtt acgcgagatg gtgagcccag aggccagcct tactgagtcg
     1621 gccatcatca acccgccgag acctggccct cgagttcggc caaccggtcg cggacgctgg
     1681 cggcctcctc gaaacgttcc gactccacca gttcgcttaa catccgctgc aaacgaacga
     1741 cctcgcggcg ctgatgttga ccgccgggag tacgcgtggg gaccttgccg aggtgctcac
     1801 cggccgtgcg gtcctccaac tcctcatcga gataggattg gaacacttgg tagcaggcgg
     1861 cgcagccgaa caacccgttc tcacggaagt cgttgagtgt agtgccgcag accgcgcaga
     1921 tcggggcgaa tccatgctcc cgctctgacg agacggcgga gatcatcgct gaggcgaccg
     1981 tgcccaacat ggagggcaaa gccacgacca aggcggcgcc gtcgacatgc ccggcctctt
     2041 cttcggcgca cgacgcacag aggtgggtgt gcgtcacggc ggaaccatcg aagtgaagca
     2101 cgtggatggt cgcgggctct tcgccacacg actcacacgt gcggccgccc gaagcggcgg
     2161 cttgccagtc ggaaccttca ctgaacacga ggcccgcctc ctctctatgc cacagcccgc
     2221 agctacgggc tgagtccatg cttgacgcgg gataggtgca aatgcctgct caggcgcata
     2281 tagcggcgct cagtgctgta acaagtgtag cacactgacc gcctcccggc cgctcccggc
     2341 ggacagaact cccctccctt ttggggggtg ccttccaggc gcagtcggcc cgaagcagag
     2401 gtcggatacg gcccgggcta ctcgggtcga agctgcggga agaggatgac gtccctgatg
     2461 gattcggcac ccaacagcag catcgccagg cgatcgatgc cgataccgag gcctccggcg
     2521 ggaggcatac cgtactcgag cgcacggagg aagtcctcgt cgaggaccat ggcctcttcg
     2581 tcgcccgccg ccttgttcag ggcctgcgct tcgaagcgcc gcctctgatc gcgcgggtcg
     2641 ttgagttcgg agaacgcgtt ccccacctcg cgcccggcga tgatcggctg gaatctctcc
     2701 accaagctcg ggtcatcggg gtgcactttc gctagagggc tgagttcgag gggatggtcg
     2761 atgacgaagg tcggctcgat gacgctcggc cacactaggt gtttgagagt cttgtcgacc
     2821 aaggaagaga aggtgtcacg gccttcgaca tcgacgccct tcacccgaag gacggagcgg
     2881 gcggcctcgg tgtcggcgcg gatgtcggcg agacccatac cgatggcacc cctcaccagc
     2941 tcgtcgaccg tgcgtcgcgg ccaagggccg cggagatcga tctcccgacc gtccacggtg
     3001 atcatggtgg accccaagac ctcttgggcc accgccggga tcatctcctc cacgagttcc
     3061 atgatctccc gatagtccac gtaggcccag tagagctcca tcatcgtgaa ctccgggttg
     3121 tggatgggcg agatgccttc gttgcggaag ttcttgccga tctcgaagac cttgtcgtag
     3181 ccgcccacca gcaaccgctt caggtacagc tcgggagcta tgcgcagata cagttccacg
     3241 tccagggcgt tgtgatgggt cgcgaacgga cgagcgagcg cccctcccgg caaaggttgc
     3301 aggatgggcg tctccacctc gaggaacccg cgctcgtaca ggtagcgccg catggcgccc
     3361 acgatcgctc cgcgcgccgt gagttgccgt ccgacctccg ggttgctgag cagatcggcg
     3421 taccgttgcc ggtaacgggt ctccttgtcc tgcagtccgt gccatttctc gggcggagcg
     3481 tggaacgact tcgtgaggag tgtccactcg gacacgaaga gcgagagttc gccccgccgc
     3541 gtgcagaacg cgtgaccttt caccccgatg atgtcgccca ggtcgagccc ggtcagcagg
     3601 tcgaacgact cctggccgac ggtgttcacg ttggcatata cctggaggtc gtcccagccg
     3661 tcgcgaagcg tcaagaaggc ggccttgccg tgctctctac gggccatgac acggcccgcg
     3721 acgcggtaga cggcgtcctc cgccgattcg ccggcaacga gatcggcgtg cgtgctcagg
     3781 acctcgtggg ccgagctgcg cggatagaac cggcgcgcga acagctcgac gcccgccgac
     3841 gagaggcggt cggccttcga ccgacgttcg cgctcgagtt cggagagatg aggagtggag
     3901 ggcactggcc cgccggtctc ctcagcgtca gaggggtggt cctcggaaga cactcaggcg
     3961 acctcgatct tcaggatctc gtacttgaga ctgccgatcg gcgccgggac ggtgacgacg
     4021 tcgcccggca cccgtccgat gatggcctgt cccatgggag attcgttcga cagcttctgc
     4081 ttcatcgggt cggcttcggc cgagcctacc agctggtaca cgagctcctc gtcgtactcg
     4141 aggtctttga gggtcacggt gacacccacg gagaccttgc ccgtggagat ctcggaatcc
     4201 ttgatcacgc gggctcgccg caacttctct tcgagctgac cgatgcggtg ttcgagcatc
     4261 gcctggtcgt tcttggcatc gtcgtactcg gagttctcgg agatatcgcc gaaatcgcgc
     4321 gcttcgcgga tgcgctcggc caccttctcc cgttccgtcg tggagagccg atcgatctcc
     4381 tcgcgaagat gccgcaatcc ctcttgtgtc agaacgactt ctttgtgatg cactggtagt
     4441 ccttcctgtc cggcactcga tccaacccgg gcaaagcagg cggggaaagc aggcggggat
     4501 tataggtgaa ccacggcacc aaagtcaaat gcagggccaa atcgagtgtt agccctgtgc
     4561 ggcggcacgt gcgaggccgg gtccgtaggc gcgccgggtg cgtgagcgcg ccggatccgt
     4621 gggcgcgccg aatcagtggg cgcgccgggt gcgtgagcgc gatggatccg gcgactagcc
     4681 ggcgatgagg gcggcgaggg cggcgtcgac ggcggcggcg tcgggcgccg ccctcagacg
     4741 gtccagatcc tcctgcgcga agccgaggcc cctcagatac cggccggcaa tcttccggct
     4801 ccagcgggcc gcgcggtgag cgcccatgtc cgcggcggcc agcgagacca gtgcccgcag
     4861 ttcggtcacc acctctatcg gtggtctccg cccctggtcg gtgcccgcca gcacgtcggc
     4921 gagcagccat gggccccgct ccgcaccgcg agcgatcatg accgcgctcg caccggtggc
     4981 ggcgacgatg gccgccgccg aagccgcgct gtccacatct cccgaggcca acacggggac
     5041 gccgaccgaa gccgccaccg ccgccgtcac cgcgtggtcc gcccg
//
LOCUS       DASRMJ010000205         5213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_17542, whole genome shotgun sequence.
ACCESSION   DASRMJ010000205 DASRMJ010000000
VERSION     DASRMJ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17542"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1546
                     /locus_tag="VFD74_05665"
     CDS             17..1546
                     /locus_tag="VFD74_05665"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZK49072.1"
                     /translation="MAEAVTGVQTLKYCIDGEWRESATEKYMPVTDSSTGRVFAETPS
                     CTAAEVEATIESAQKAFETWSAKPASVRTQVLFRFRALVEEHMEELTFSTSREVGKNL
                     DEARGEIVKIIEACEVAVAAPMLMKGESLMNVSTGHDTVTYREPLGVFAGIAPFNFPS
                     MIPFGWMVPLCIATGNTMVIKAPSMVPTSSMRLLELLYEAGLPNGVVNLVTAGRKEAE
                     IMMTHPLIRGINFVGSTSVGLHVYSVAAAHGKRVQAQTEAKNHGLVLADASLERAARG
                     IINSTFGCAGMRCMALPVCVVEDAVADEFVGYMKQFAAERVVGCSYDPATELGPVVSA
                     EHQQFIKGWIDKAEQEGADLILDGRDISVPGYEGGFFVGPSIFDHVTEDMGCGREEVF
                     GPVLFVKRVSGFEEGITLMNNSRFANGSCIFTESGYYAREFTRRTHGGMVGINVGIPV
                     PSSFFPFSGHKQSFFGESHVLGTDGIKFYTDTKSVTSRWFTEDDKATTKVSTWEGTIT
                     R"
     gene            complement(1659..2672)
                     /locus_tag="VFD74_05670"
     CDS             complement(1659..2672)
                     /locus_tag="VFD74_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZK49073.1"
                     /translation="MTDRYASLGPVAVIGAGLTGASWAALFAAHGREVCLFDVDRERA
                     ESACGRALEMGRFLVEKELAQADAFALGSSRLWAAATMEEALAAAVHVQECVAEDLEV
                     KRSVFAQADRAARPDAIIATSSSGLSITDIQAGLPGAGRCIAAHPYNPPHLVPLVELA
                     PGAETDPAVMERARDFYLSVGKDPVVLSRDVPGYLSNRMSAALWREAIELVRTGVASV
                     ADVDRAISSGPGLRWAAMGPHLLYHLGGGEGGIRGHVEHLGHVKEGMLRDIATWTTFP
                     SDTVDVLEEGLREVLADAEPGVLERERDEALAGFILARARAARLRARAGPAGAAEPAR
                     RPD"
     gene            complement(2676..4574)
                     /locus_tag="VFD74_05675"
     CDS             complement(2676..4574)
                     /locus_tag="VFD74_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HZK49074.1"
                     /translation="MPKSYAEEFRRSIEEPEAFWAEAAAAVHWDRQWDTVLDDSAAPM
                     YRWFVGGRLNTCYNALDYHVESGRGDQKALIYDSPVTDTVRSYTYRELTSEVARFAGA
                     LRGLGVTKGDTVVIYMPMVPEAVIAMLACARIGAVHSVVFGGFAPQELAVRIDDAKPR
                     VVVSASCGIEVSRVIEYKPLLDRAIEFAECKPESCVILQRPMATASLVEGRDHDWVDL
                     MAEAAAVPCVSVEATDPLYILYTSGTTGKPKGVVRDNGGHAVALRWSMENIYDVHPGD
                     VYWAASDVGWVVGHSYIVYAPLFTGCTTILYEGKPVGTPDAGALWRVIRDHGVKVLFT
                     APTAFRAIKKEDPTGEYLKKYDISHFKYLFLAGERLDPDTYFWASELLKVPVIDHWWQ
                     TESGWPIAANCMGLEPFPVKAGSPTKAVPGYDVRILDAEAHPVPAGAEGIVTIKLPLP
                     PGCLPTLWQDDARYVESYVSAYPGFYFTGDGGYIDEDGYVYVMGRVDDVINVAGHRLS
                     TGRMEEVIAQHPDVAECAVLGVADELKGQVPIGLCVLKAGVEHSDSDIVGDVVRMVRD
                     QVGAVASFKAATVVQRLPKTRSGKILRGTMRKIADSQPFTTPSTIDDPAILGEIAERM
                     RTIGYAKE"
     gene            complement(4747..>5213)
                     /locus_tag="VFD74_05680"
     CDS             complement(4747..>5213)
                     /locus_tag="VFD74_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49075.1"
                     /translation="TIQEVLDELGAGHKTRVVAYNKVDLVEAATGRAFSIPHGDRHAV
                     GIAALDGRGLDGLRTTLGSVLAEMWAEVDLTVPYTAGSLLARLRERGAVDFEYRDTEV
                     RVHGRVDQALAGELVQAARSWRRVVKEAGLERLRAGSPRATDDDQDPGEQVE"
BASE COUNT          870 a         1845 c         1645 g          853 t
ORIGIN      
        1 gaggaggagc atcgagatgg cagaagctgt gaccggagtg cagaccctga aatactgcat
       61 cgatggcgag tggcgcgagt cggcgacgga gaagtacatg ccggtgaccg actccagcac
      121 cggccgcgtg ttcgccgaga ccccctcgtg caccgccgcc gaggttgagg ctacgatcga
      181 atccgcccag aaggccttcg agacctggtc cgccaagccg gccagcgtgc gcacccaggt
      241 gctgttccgc ttcagggcgc tcgtcgaaga gcacatggaa gagctcacct tctccaccag
      301 tcgcgaggtc ggcaagaacc tcgacgaagc ccgcggcgag atcgtcaaga tcatcgaggc
      361 ctgcgaagtg gctgtggccg cgccgatgtt gatgaagggc gagagcctga tgaacgtgtc
      421 caccgggcac gacaccgtca cctacaggga gccgttgggc gtcttcgcgg gcatcgcgcc
      481 tttcaacttc ccctccatga tcccgttcgg ctggatggtg cccctgtgca tagccaccgg
      541 caataccatg gtgatcaagg ctcccagcat ggtccccacc tcgtctatgc gcctgctcga
      601 actgctctac gaggccggac tccccaacgg cgtggtcaac ctcgtcacgg cgggccgcaa
      661 agaagccgag atcatgatga cccatccgct gatcaggggc attaacttcg tgggctcgac
      721 ctccgtcggc ctgcacgtct actcggtggc cgcggcccac gggaagaggg tccaggcgca
      781 gaccgaagcc aagaaccacg gcctcgtcct ggccgacgcc tccctcgagc gcgcggcccg
      841 cggcatcatc aactccacct tcggctgcgc cggcatgcgc tgcatggcct tgcccgtatg
      901 cgtcgtcgag gacgccgtcg cggacgagtt cgtcggctac atgaagcagt tcgccgctga
      961 gcgggtagtc ggctgctcct acgacccggc gaccgaactc ggccccgtcg tcagcgccga
     1021 gcaccagcag ttcatcaagg gctggatcga caaggcggaa caggaaggcg cggacctcat
     1081 cctcgacggt cgcgacatct ccgttcccgg ctacgaaggt ggcttcttcg tcggccccag
     1141 catcttcgac cacgtgaccg aggacatggg ctgtggccgc gaagaggtct ttggcccggt
     1201 cctgttcgtg aagcgcgtgt ccggcttcga agagggcatc accctcatga acaacagccg
     1261 cttcgctaac ggttcctgca tcttcacaga gagcggctac tacgctcgcg agttcacgcg
     1321 ccgcacgcac ggcggcatgg tgggaatcaa cgtaggcatt ccggtgccgt cgtccttctt
     1381 cccgttctca ggtcacaagc agtcgttctt cggcgagagc cacgtgctcg gcaccgacgg
     1441 tatcaagttc tacaccgaca ccaagagtgt cacgtctcgc tggttcaccg aagatgacaa
     1501 ggcgaccacg aaggtctcca cgtgggaggg gaccatcacc cgctagtaga cgcgcccatc
     1561 gggtcgtcgc gcccacggtc gcacgaaccc ctcggacccg gctgccggac cgccttgtgg
     1621 cgggacccgc agccgggttc ttcctattgc cgggcacctc agtccgggcg ccgggcaggc
     1681 tcggcggcgc cggccgggcc ggcccgggcc cgcagcctgg ccgctcgggc ccgggccagg
     1741 atgaagcccg ccagagcctc gtcacgctct cgttccagca cgcccggctc ggcgtccgcc
     1801 agcacctcac gcagaccctc ttccagcacg tcgaccgtat ccgaagggaa agtggtccac
     1861 gtggcgatgt cacgcagcat gccctccttc acgtggccca gatgttccac gtggccccgg
     1921 atgccaccct cgccgccgcc gaggtggtac agcaggtgag ggcccatagc ggcccaacgg
     1981 agtccgggcc cgctcgagat ggcgcgatcg acgtcggcca ccgacgccac gcctgttcgc
     2041 accagttcga tcgcctcgcg ccacaacgcc gccgacatcc tgttcgatag atagccgggc
     2101 acgtcccggg acaacaccac ggggtccttg cccaccgaaa ggtagaaatc ccgggcgcgc
     2161 tccatgactg cggggtcggt ctcggcgccc ggggccagct caaccagagg aaccagatgt
     2221 ggcgggttgt atgggtgtgc ggcgatgcac cgtccggcgc caggcaggcc cgcctggatg
     2281 tcggttatgg agaggccgct ggaactcgtg gcgatgatcg cgtcaggccg ggcggccctg
     2341 tcggcctggg cgaagacgga cctcttcacc tccaggtcct ccgccacgca ctcctgcacg
     2401 tgtacggccg ccgcgagcgc ctcctccatc gtcgccgccg cccacagtcg ggacgagccc
     2461 agcgcgaacg cgtccgcctg ggcgagctcc ttctccacga ggaagcggcc catctcgagc
     2521 gcccgcccgc aggccgactc cgcccgctcc cgatccacgt cgaagaggca tacctcccgt
     2581 ccgtgtgcag cgaagagggc agcccaactc gcgcccgtga ggcccgcgcc gatcacggcc
     2641 accggcccca gcgacgcata ccgatctgtc acaggctact ccttggcgta cccgatcgtg
     2701 cgcatacgct cggcgatctc tcccaggatg gcggggtcgt cgatggtgga cggtgtggtg
     2761 aagggttggc tgtcggcgat cttgcgcatg gtgccgcgca ggatctttcc agaacgggtc
     2821 ttggggagcc gttgcaccac cgtggccgcc ttgaacgacg cgaccgcacc gacctggtct
     2881 cgcaccatcc tgacgacatc gccgacgatg tcagaatccg aatgctcgac ccccgccttc
     2941 agcacgcaga gcccgatggg gacttgcccc ttcagctcgt ccgccactcc gagcaccgcg
     3001 cactccgcca cgtcgggatg ctgcgcgatg acctcttcca tgcgcccggt ggacagccgg
     3061 tggcccgcca cgttgatgac gtcgtcgacc ctgcccatca cgtatacgta gccgtcttcg
     3121 tcgatgtagc cgccgtcgcc ggtgaagtag aagccgggat aggcgctcac atacgactcg
     3181 acgtagcgtg cgtcgtcctg ccagagagtc ggcaggcagc cgggaggaag cggcagtttg
     3241 atggttacga tgccctctgc gccggcgggc accgggtgcg cctcggcgtc caagatacgc
     3301 acgtcgtagc cgggcacggc cttggtggga gaccccgcct tgacggggaa cggctcgagg
     3361 cccatgcagt tcgctgcgat gggccaaccg gattccgtct gccaccagtg gtcgatcacc
     3421 ggcaccttga gcagttctga ggcccaaaag tacgtgtcgg gatccagacg ttcacccgca
     3481 aggaagaggt acttgaaatg tgagatgtcg tacttcttca ggtactcccc cgtcgggtct
     3541 tccttcttga tagcccggaa cgccgttggg gcggtgaaca ggaccttcac accgtggtcg
     3601 cgtatcacgc gccagagcgc accggcgtcg ggggtgccca cgggcttgcc ctcgtagagg
     3661 atggtggtgc agcccgtgaa cagaggggcg tatacgatgt agctgtgccc caccacccag
     3721 ccgacgtccg aagcggccca gtagacgtcg cccgggtgga cgtcgtagat gttctccatg
     3781 gaccagcgga gcgcgaccgc atgcccgccg ttgtcgcgca ccacaccctt cggcttgccc
     3841 gtcgtgcccg aggtgtagag gatgtagagc gggtccgtag cctccaccga gacgcagggt
     3901 acagccgcgg cttccgccat caagtccacc cagtcgtgat cccggccctc gaccagggag
     3961 gccgttgcca tgggccgctg caagatcacg caactctccg gcttacactc cgcgaactcg
     4021 atcgccctat cgaggagggg cttgtactcg atcacgcgcg acacctcgat gccgcacgag
     4081 gccgagacca caaccctggg tttcgcgtcg tcgatgcgca ccgccagctc ctgcggggcg
     4141 aaaccgccga acaccaccga gtgcaccgct ccgatgcgcg cgcaggccag catggcgatg
     4201 acggcttcgg gcaccatggg catgtagatg accaccgtat cgcccttcgt cacgcccaat
     4261 cccctcaggg cgccggcgaa ccgggccacc tccgaagtga gttcgcgata ggtgtaggag
     4321 cgcacagtgt cggtgaccgg gctgtcgtag atgagggcct tctggtcgcc ccgccccgac
     4381 tcgacgtggt agtcgagcgc gttgtagcag gtgttgagtc ggccccccac gaaccacctg
     4441 tacatcggcg ccgcgctgtc gtcgagcacc gtgtcccact gcctgtccca gtggacggcc
     4501 gcggcggcct ccgcccagaa ggcctccggt tcctcgatgg atcgccggaa ctcctcggcg
     4561 tagctcttcg gcatcacgat cccctttctt cgaacgcggg gtaggtccgg gcaggatatt
     4621 ccgtacccgc atcgccagtc aacgtatgtc ccgccgggcg gtgctccctg ggggatgacc
     4681 ggtcacgccc tcgtggctgg gcgcacgcgc cgcggacggc ggcgcccggc cgtgacgcag
     4741 ccccggctac tccacctgct cgccgggatc ctggtcgtca tcagttgcgc gcgggctccc
     4801 agcgcggagc cgctcgagtc cggcctcttt gaccacccgc ctccaactcc gcgccgcttg
     4861 gacgagctcc cccgcgagag cctggtcgac ccgcccatgc acacgcacct ccgtgtcccg
     4921 gtactcgaag tcgacggcgc cgcgctcacg gaggcgcgca agcaacgatc ccgccgtgta
     4981 ggggacggtc aggtccacct cggcccacat ctcggcaagc acgctcccca gcgtcgtgcg
     5041 cagtccgtca aggccgcggc cgtccagggc ggcgatcccc acggcgtgcc tatcaccgtg
     5101 agggatggag aaggcccgcc ccgtcgccgc ctccacgagg tctactttgt tgtacgcgac
     5161 cacccgagtc ttgtgtcccg ccccgagttc gtctaacacc tcctggatgg tac
//
LOCUS       DASRMJ010000206         5021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_418597, whole genome shotgun sequence.
ACCESSION   DASRMJ010000206 DASRMJ010000000
VERSION     DASRMJ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418597"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /gene="mqnB"
                     /locus_tag="VFD74_05685"
     CDS             complement(<1..683)
                     /gene="mqnB"
                     /locus_tag="VFD74_05685"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HZK49076.1"
                     /translation="MVDHAIPRRRPGALELHELLADVELAVQAATTLEAHIVLDCLSG
                     AEHLAVAGRPWWVGDLPASARTVRTVVLVSGYDKVNVAHSLTCLLQAGSPRLVIQTGV
                     AGGFVSSGIQVGDLALAASDTYADLGVLAPEGWLSADAFAEPVALVDGVELRNTFPLD
                     PGLVDASARIFRAASWPGRTPRVRVGPFLTSSQVTGTSARAELLDARWGGIAESMEGA
                     AAAHVCALYG"
     gene            complement(700..2097)
                     /locus_tag="VFD74_05690"
     CDS             complement(700..2097)
                     /locus_tag="VFD74_05690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZK49077.1"
                     /translation="MAFEFKFPDVGEGITEGELLAWSVKVGDVVAEDQTLAEVETDKA
                     VVEMPSPRAGRIAKLHAEEGETIEVGQVIVTIDEEAGAPASAKIGVRVPSGAAPAPAA
                     APHLGHAGEEEEEVEFYTGSVIGHLEEASDEPEDVAVAGSVAGGRAGDEDSAIKVAAM
                     PSVRSLAKELHVDLGMVHGTGPGGRILRQDVEDHAEASVYGRPSVSAPAVPLRELESA
                     EAWMGGAIARDEHGVVERVTFKGVRRTMARRMAESLAKQAQVTTTDEADITVIRRIRE
                     KERTLAAERGLRLTYLAFVIKAVAAALQRFPRMNATLDESCEQLVLKRYYNVGVAIDT
                     KAGLLVPNIKNADAKGIFQLAGEIADLVERAQDRRIELAELRGGTFTVTNYGAIGGLY
                     ATPVTNYPEISILGMGRVHDAPVVHRGEVVVRQILPLSLTYDHQVIDGAEAARFLNLV
                     ISYLEDPDLMLLEGL"
     gene            complement(2100..3080)
                     /locus_tag="VFD74_05695"
     CDS             complement(2100..3080)
                     /locus_tag="VFD74_05695"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006054581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZK49078.1"
                     /translation="MARLNMVEAIDQALTQEMERDPTVVVLGEDVGKDGGVFRVTDGL
                     VERYGESRVIDTPLAESGIVGFAIGMAIGGLRPVAEIQFSGFVYPAYDQLISHAARFR
                     NRTRGQRTVPLVVRMPYGGGIRALEHHSESMEAVFAHIPGLKVVIPSDPYEAKGLLTA
                     AIRDPDPVVFFEPARVYRAIKTEVPEEQYTIPLGKAHVVRGGADVTLISWGAHVRTIR
                     EAAERLSADRGYDVEVIDLRTLSPFDSETVTRSVAKTGRAVVVQEAPISGGFASEVSA
                     QLMEREILHLRAPVKRVAGFDTVPPLSKLEDYYQPSVDRVVDEVIATMEF"
     gene            complement(3082..4161)
                     /gene="pdhA"
                     /locus_tag="VFD74_05700"
     CDS             complement(3082..4161)
                     /gene="pdhA"
                     /locus_tag="VFD74_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HZK49079.1"
                     /translation="MPVTTLVTYSVDRIEVMDTAGEVDAALMPDLSPEKIRALYDLMV
                     LTRRFDERMLKLQRQGRMGTFARVAGQEGAHVGAAFALRDDDWMVPSFREAGAFLARG
                     MDMMRFLQYWAGDERGNVFPEGVRMLPASIPVGTHMLHAVGLGWAMRHRGEQAAVLTV
                     FGEGATSEGDFSEAMNFAAVFQAPVVFLCQNNQWAISVPYHKQTQACSVAQKAVAYGM
                     PGVQVDGNDVFAVYGTVREALDRARGGGGPSLVEAHTYRVTDHTTADDARRYRDEAEV
                     VTWRERDPIARLTAYMKTQGHLDEAAEQAAWAKADATVAEAVTAFEALDPPGPEEIFA
                     HVFAEITPPLAEQRARLLARLQEGS"
     gene            complement(4524..>5021)
                     /locus_tag="VFD74_05705"
     CDS             complement(4524..>5021)
                     /locus_tag="VFD74_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein containing YHS domain protein"
                     /protein_id="HZK49080.1"
                     /translation="AAPGLPWESARGAQSEGGLSIGISPALSLHEHVNFYKSPADHFD
                     VMIYTGSGLMGREIVNIRSSDMVAIINGRSGTLGEFSIAYDEAKLIGVVKGSGGIADI
                     IPQLVHAIEKDTGAVILYDSDPAALVDRMIGEYVGRHFKHPSILVQNGYEKKGGTRSR
                     EAGEG"
BASE COUNT          842 a         1820 c         1505 g          854 t
ORIGIN      
        1 ccgtagagcg cgcacacatg cgctgcggcc gcaccctcca tcgactccgc gatcccgccc
       61 caccgggcgt cgagaagctc cgctcgagcg ctcgtaccgg tgacctgcga tgacgtcagg
      121 aagggtccga ccctcacccg cggcgtcctg ccgggccagc tcgccgccct gaatatgcgc
      181 gctgacgcat ccaccaaccc cgggtcgagc gggaaggtgt tacgcaactc cactccgtcc
      241 accaaggcca ccggctcagc gaaggcgtcg gccgacagcc acccctccgg cgccaagact
      301 cccaggtcgg cgtacgtatc cgacgccgcc agggccaagt ctcccacctg aatacctgac
      361 gagacgaacc ccccggccac cccggtctgg atcaccaacc taggagagcc cgcctgcagc
      421 aggcacgtga gcgaatgagc cacgttgacc ttgtcgtatc ccgagacgag caccaccgta
      481 cgcacggtgc gggcggaggc cggcaggtcc cccacccacc agggtctccc cgcgaccgcc
      541 agatgctcgg caccgctcag gcagtcgaga acgatgtgcg cctccagcgt cgtggccgct
      601 tgcacggcca gctcgacgtc cgcgagaagc tcgtgaagct cgagcgcacc cggtcgccgg
      661 cgggggatgg catggtccac cacggccctc gatcccgtcc tagagccctt cgaggagcat
      721 gagatccgga tcctcgaggt agctgatcac caagttcaag aagcgtgccg cttcggcccc
      781 atcgatcacc tgatgatcat aggtgagcga cagcgggagt atctgcctga cgacgacctc
      841 gcccctgtgc accacggggg catcgtggac cctccccatt ccaaggatgg agatctccgg
      901 gtagttggtg accggcgtcg cgtacagacc gccgatggcg ccgtagttgg tcacggtgaa
      961 ggtgcccccc cggagctccg cgagttcgat gcgccggtcc tgcgctcgct ccaccaggtc
     1021 ggcgatctcc ccggccaact ggaagatgcc cttggcatcc gcgttcttga tgttggggac
     1081 cagcaggccc gctttcgtgt cgatggcgac gccgacgttg tagtatcgct tcaacaccag
     1141 ttgctcgcag ctttcgtcca aggtcgcgtt catacgcggg aacctctgca acgcggccgc
     1201 cacggccttg atcacgaacg ccaagtaggt gagcctgagc ccccgctcag cggccaaggt
     1261 cctctccttc tcgcggatcc gacgtatgac ggtaatgtct gcttcatcgg tggtggtcac
     1321 ctgtgcctgc ttggccagcg actcggccat ccgccgagcc atcgtgcggc gaacgccctt
     1381 gaacgtcacg cgctccacta ccccgtgttc gtcccgcgcg atggcgcccc ccatccacgc
     1441 ctcggccgac tccaactcgc gcaacggcac ggccggagcc gagaccgagg gtcggccgta
     1501 gaccgaggcc tctgcgtgat cctccacgtc ctggcgaagg atgcggcctc cgggacccgt
     1561 gccgtgcacc atccccagat ccacgtggag ttccttcgcc agcgaacgca ccgagggcat
     1621 cgccgcgacc ttgatcgccg agtcttcgtc accggcgcgc ccgcccgcga ccgagccggc
     1681 gacagcgaca tcttcaggct cgtccgaggc ctcctcgagg tgacctatca ccgacccggt
     1741 gtagaactcc acctcttcct cttcttcgcc ggcgtggccg aggtgcggag cagcggccgg
     1801 cgcgggcgcg gcaccggacg gcacccgcac cccgatcttc gccgacgcgg gcgccccggc
     1861 ttcctcgtcg atcgtcacga tcacctgccc cacttcgatg gtctcgccct cctcggcgtg
     1921 gagcttcgcg atgcggcccg cgcgggggga gggcatctcg accaccgcct tgtcggtctc
     1981 gacctcggcg agcgtttggt cttcggcgac gacgtcgccc accttcacgc tccatgcgag
     2041 aagctctccc tcggtgatcc cctcgcccac atccgggaac ttgaactcaa acgccatggc
     2101 tagaactcca tcgtcgcgat gacctcgtcg accactcgat cgacgctggg ctggtagtag
     2161 tcctccaact tggacagcgg cgggactgta tcgaatcctg ccacccgctt caccggcgcc
     2221 ctgaggtgca ggatctccct ctccatgagc tgcgcggata cctccgaggc gaaccccccg
     2281 ctgatcggcg cttcttggac cacgaccgct ctccccgtct tggccacact gcgcgtgacc
     2341 gtctcggaat cgaacgggga cagagtccgc agatcgatga cctcgacatc gtagcctctg
     2401 tcagccgata gacgctccgc cgcttctctg atggtccgca cgtgagcccc ccacgatatc
     2461 agggtgacgt cggcgcctcc gcgcaccacg tgcgccttcc ccagaggtat cgtgtactgc
     2521 tcttcgggga cctctgtctt gatcgccctg tagacccgcg ccggctcaaa gaacaccacg
     2581 gggtccgggt cgcggatggc ggcggtgagc agacctttcg cctcgtaggg atcggagggt
     2641 atgacgacct tcagccctgg gatgtgggcg aagacggcct ccatgctctc cgagtggtgc
     2701 tcaagagcgc ggatcccgcc accgtatggc atgcgcacca ccagcggcac cgtcctctgg
     2761 ccacgggtcc gattgcggaa ccgcgcggcg tgagagatga gctggtcgta cgccgggtac
     2821 acgaagccgg aaaactggat ttccgcgacc ggccgaaggc ccccgatcgc catgccgata
     2881 gcgaacccca caatacccga ctccgcgagc ggggtgtcga tcacgcggga ttctccgtag
     2941 cgttccacca gcccgtcggt gacgcgaaac acccctccgt ccttgcccac gtcctcgccc
     3001 aacaccacaa cggtcgggtc tcgctccatc tcctgggtga gagcctggtc gatggcctcg
     3061 accatgttga gccgggccac ctcagctccc ctcctgcaga cgagccagga gccgggcgcg
     3121 ctgctcggcc aacggaggtg tgatctccgc gaacacgtgg gcgaagatct cctccggccc
     3181 ggggggatcg agagcctcga aggcggtcac ggcctcggcc accgtggcgt cggccttggc
     3241 ccaagccgcc tgctcggccg cctcgtccag gtgcccctgc gtcttcatgt aggccgtcag
     3301 gcgggcgatc gggtcgcgct cgcgccacgt gaccacctcc gcttcgtcgc ggtagcggcg
     3361 ggcgtcgtcg gccgtggtgt gatcggtgac cctgtaggtg tgcgcctcga cgagcgaggg
     3421 cccgccgccg ccccgagccc gatcgagggc ctcgcgcacc gtcccgtaca cggcaaacac
     3481 gtcgttgcca tccacctgca cgcccggcat gccgtaggcc acggccttct gcgccaccga
     3541 acacgcctgg gtctgcttgt ggtaggggac ggatatggcc cactggttgt tctggcacag
     3601 gaacaccacc ggtgcctgga acaccgccgc gaaattcatg gcctcgctga agtccccctc
     3661 ggaggtggca ccctctccga agacagtgag cactgcggcc tgctcgcccc tgtgcctcat
     3721 ggcccatccc aaaccgaccg cgtggagcat gtgcgtgccc accggtatcg aagccgggag
     3781 catgcgaacg ccttcgggga acacgttgcc gcgctcgtca ccggcccagt actgcaggaa
     3841 ccgcatcatg tccattcccc gcgccaggaa cgcgcccgct tcacggaacg acggcaccat
     3901 ccagtcatcg tcgcgcaatg cgaaggccgc ccccacgtgc gccccctcct gcccagccac
     3961 ccgggcgaac gtgcccatgc ggccctggcg ctgcaacttc agcatgcgtt cgtcaaagcg
     4021 ccgcgtgagc accatgagat cgtacagagc acggatcttc tcgggagaaa gatcgggcat
     4081 gagagctgcg tcgacctcgc cggcagtatc catcacctcg atccgatcga cggagtacgt
     4141 caccaacgtg gtgaccggca tgttccacct ccccgggttg cctgcagcaa ccatatacca
     4201 cgtggaggcc aacgaatacg cacaactgga tcgatgctat acttgccgtc ggccatgcgg
     4261 cttcgtccgc gattcgtggc ccggaaggag gtgtggatat atgaaccaca ctcaaacgtg
     4321 cggccactgt cgcaccgaga tccctctctt cgcttgggtc tgtcccagct gcgaggcgtg
     4381 ggtcggggaa atcacccgac acgacgctca ggggtttcga acggagccga tctccggatg
     4441 actgtccgag atcactccgg gacctagggt ccgaacaagg agagccccac gggggctttt
     4501 tttgttgggc cggctacctc tccctatcct tctcccgctt ccctcgaccg cgtgccgccc
     4561 ttcttctcgt agccgttctg caccaagatg gacgggtgct tgaagtgccg accaacgtac
     4621 tccccgatca tccggtcgac cagcgctgcc ggatcggaat cgtacaggat gaccgcgccc
     4681 gtatccttct cgatcgcgtg caccagctga gggattatgt cggcgatgcc gccgctcccc
     4741 ttcacgaccc caatcaactt cgcttcgtca taggcgatcg agaactctcc gagggtcccc
     4801 gagcggccgt tgatgatggc gaccatgtcg ctcgagcgga tattgacgat ctcgcggccc
     4861 atgagcccgc ttccagtgta gatcatgacg tcgaagtgat ccgccggcga cttgtagaag
     4921 ttcacgtgct cgtgaaggct gagagccgga gagatgccta tcgagagccc tccctccgac
     4981 tgcgcgccgc gggccgactc ccaagggagt cccggcgccg c
//
LOCUS       DASRMJ010000207         4886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_136380, whole genome shotgun sequence.
ACCESSION   DASRMJ010000207 DASRMJ010000000
VERSION     DASRMJ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136380"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFD74_05710"
     CDS             <1..711
                     /locus_tag="VFD74_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49081.1"
                     /translation="VLVLAIIIGGAAFVAFGTDRTPWDFGGALRTTRIDGPASAGVRE
                     AEARIDVGAARIDIASSRGGVMTEGTLESRRDPQVTHNVSNGVYTLEVLQKKGVQIFH
                     SGIQGDRLVLDLAQGVPWKIDVDTGAADANLDLRGVTLSELTVDAGASSLDLTLGPDI
                     VGGARVSVDGGAGSYRLRLPRDLDITLTIDAGVSSVNVDGAFQRTGDVYRLAGAGAGE
                     ALSVDINAGVSSISVELY"
     gene            complement(792..1181)
                     /locus_tag="VFD74_05715"
     CDS             complement(792..1181)
                     /locus_tag="VFD74_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HZK49082.1"
                     /translation="MTEAIGGSIDGLPAGSPAAAGDRCIACGYAKLPRQTAAAQVYQM
                     VTIVTLVDKPTNVVLEASVTLITPLARSFVETLLVGSNLEADQERILQELRVNYGGGA
                     QKAVKQAYRDLCERYAEMKAAGGTYHR"
     gene            complement(1320..2096)
                     /locus_tag="VFD74_05720"
     CDS             complement(1320..2096)
                     /locus_tag="VFD74_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZK49083.1"
                     /translation="MAFVAVNVEGRVATVTLDRPEALNAFNSQVLQELLQEFVALGHR
                     EDVGAIVLTGSGRKAFAAGADIAEMRDKSPDEARSFAELGQMVTTTIERIPQPVIAAV
                     NGYALGGGCEMALACDIRLCSDNARFGQLEVNLGIIPGWGGSQRLARLCGPAFARDLI
                     YTGRVADPQEALRWGLVNAVHSQDELMAKAYEMAETIAGKSGVIVQYAKACINRAFDQ
                     DLAGGLRLEAGFFGLCFATEDQKEGMSAFLEKRPAQFKHR"
     gene            complement(2194..2598)
                     /locus_tag="VFD74_05725"
     CDS             complement(2194..2598)
                     /locus_tag="VFD74_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HZK49084.1"
                     /translation="MSGKIRVVIAKPGLDGHDRGAKVIARALRDAGMEVIYTGLHQTA
                     EQIVETAIQEDADVVGLSILSGAHLTLVPKVVRILRGQGIDDIVVLAGGTIPDEDHDE
                     LKREGVAAIFTPGATTGEIIEYITSVATGSAA"
     gene            complement(2666..4324)
                     /locus_tag="VFD74_05730"
     CDS             complement(2666..4324)
                     /locus_tag="VFD74_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZK49085.1"
                     /translation="MDDVRDGSRTKAEWLAGAYSWQNTRDADFTSVSSAEVEPIYDPG
                     DRVGHDYHRELGYPGEYPYTRGVHPTMYRGHLWTMRQFAGFGGAEDTNGRFRFLLDQG
                     QTGLSLALDLPTLMGRDSDDPRSEGEVGRCGVAMDSVLDMERVFEGIPLDQVTVSMTT
                     NAPATILLAFYLVAAERQGVKHDQVGGTIQNDILKEYIAQNTYIFPPEPSLRLITDIF
                     AFCGEHVPQWNTISISGYHIREAGATAAQELAFTLADGFAYVEAGIAAGLEVDSFAPR
                     LSFFFNAHIDLFEEVAKFRAARRIWARHMRERYRAKDPRSWLMRFHTQTAGCSLTAQQ
                     PENNVVRTTIEALAGVLGGTQSLHTNSLDEVLSLPTERAAEIALRTQQVLAYESGVAN
                     VIDPLAGSYYVESLTDRMEAEAEEYFIKIEELGGVVAAIAKGFFQQEIADASYRYQMA
                     VESGRKVVVGVNRFATGVRPAVETLRIDKEFEMRQVDSLRALRERRDASPVARGLEDL
                     RAACADGTNVMPPILECTRAMCTLGEMVATMKSVFGVYQGSAVF"
     gene            complement(4373..>4886)
                     /locus_tag="VFD74_05735"
     CDS             complement(4373..>4886)
                     /locus_tag="VFD74_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZK49086.1"
                     /translation="ALTFDGVRVHESARLGAEGEGFSIAMKTLDGGRIGIAAQAVGIT
                     QAAFDAASRYAKERRQFGRRIADFQAIRWKLADMDKDLEAARLLTHKAACLKMNGSSA
                     TVAGARAKLFASEMARKHTGEAVQILGGYGYTKEFPVERYYRDAKITEIYEGTSEVQR
                     IIISRASLGR"
BASE COUNT          776 a         1669 c         1572 g          869 t
ORIGIN      
        1 gtcctcgtgc tggcgatcat catcgggggc gcggcgttcg tggccttcgg caccgatcgc
       61 actccttggg atttcggggg ggctctccga accaccagga tcgacggtcc cgcctccgcg
      121 ggcgtgcgcg aggctgaagc acgtatcgac gtaggcgccg cgcgaatcga catcgcgtcg
      181 agtcgggggg gcgtcatgac cgagggtacc ctcgagtctc gccgcgaccc gcaggtgacg
      241 cacaacgtgt ccaacggcgt ctacaccctc gaggtgctgc agaagaaagg cgtgcagatc
      301 ttccactcgg gcatccaagg ggacaggttg gtccttgacc tggcgcaggg tgtgccttgg
      361 aagatcgacg tggatacggg agccgccgac gccaacctcg atctgagagg cgtgacactc
      421 agcgagctca ccgtggatgc gggcgcgagc tccctcgacc tgaccttggg ccccgatatc
      481 gtcggcggcg cgcgtgtgag cgtcgacggg ggcgccggct cgtatcgtct tcggctgccg
      541 cgcgacctcg atatcacgct caccatcgac gcaggtgtct cttcggtgaa tgtggacggc
      601 gccttccagc gaaccggcga cgtctaccgg ctcgccggcg ccggcgccgg cgaagccctc
      661 agcgtagaca tcaatgcggg cgtgagctcg atctcggtcg aactctactg acgccccgag
      721 cggggcgaac ggctcggacc ggcggagaaa ccgacttctc cgccggtccg ccggtgcgtc
      781 gaatcacaag tctagcggtg gtaggtgccg cccgccgcct tcatctccgc gtagcgctcg
      841 cacagatccc ggtaggcctg cttgaccgct ttctgcgcac ccccaccgta gttcacgcgc
      901 aactcctgga gaatccgctc ctggtcggcc tcgaggttgg agcccacgag gagggtctcg
      961 acgaacgaac gcgccaacgg cgtgatgaga gtcaccgagg cctcgagcac cacgttggtg
     1021 ggcttgtcca ccaacgtcac gatcgtgacc atctggtaca cctgggcggc tgcggtctgc
     1081 cgtggcagct tcgcgtaacc gcatgcgatg caccgatccc ctgcagccgc cggactgccc
     1141 gcgggtaggc cgtcgatcga cccgccgatt gcttccgtca tggcgccacc ttcccttcca
     1201 gtgccacccg tgcccgatcg ggcacatcta tcggctcgaa cgcggcggtc tcgcgcaggt
     1261 gtgtgtgggt ccacccgtcg aacctcggcg ccgagccggt ctcgctcacg ccgttcggct
     1321 cagcgatgct tgaactgggc cggacgcttc tcgaggaagg cactcatgcc ctccttctgg
     1381 tcttcggtag cgaagcagag cccgaagaag cccgcctcca gacgcagccc tcccgctagg
     1441 tcttggtcga acgcgcggtt gatgcacgcc ttcgcatatt gcacgatcac tccgctcttg
     1501 ccggcgatgg tctcggccat ctcgtacgct ttggccatca gctcgtcctg cgagtggacg
     1561 gcgttcacca gcccccagcg tagcgcttcc tgcggatcgg cgacgcgacc tgtgtagatc
     1621 aggtcgcggg cgaaggccgg cccgcagagg cgcgccaggc gctggctgcc tccccacccg
     1681 ggaatgatgc cgaggttgac ctccaactgg ccgaaacggg cattgtcgga gcagagccgg
     1741 atgtcgcagg ccagagccat ctcacacccg ccaccgagcg cgtagccgtt gaccgccgcg
     1801 atgacgggct gggggatcct ctcgatcgtg gtggtgacca tctgaccgag ttccgcgaac
     1861 gaccgcgcct cgtcgggcga cttgtcgcgc atctcggcga tgtcggcgcc ggcggcgaag
     1921 gccttccggc ccgacccggt gaggacgatc gcgcccacgt cctcacggtg gcccagcgcc
     1981 acgaactcct ggagaagttc ctgcaacact tgggagttga aggcgttcag cgcctcgggc
     2041 cgatcgaggg tgaccgtggc cacccgaccc tctacgttca ctgccacgaa tgccatggtg
     2101 gatccctccg tgctcgttcg gccaccccca agggagcggc cgagataggt ggtgtgaggc
     2161 ccgctcctac ggaggggccc tgtgcggccg ccgctaggcg gcgctcccgg tcgcgaccga
     2221 ggtgatgtac tcgatgatct cgccggtggt ggcgcccggg gtgaagatgg cggctactcc
     2281 ctcgcgcttg agttcgtcgt gatcttcgtc ggggatcgtg ccgccggcaa gcaccacgat
     2341 gtcgtcgatg ccctgcccgc gaaggatccg caccaccttc ggcaccaagg tgagatgggc
     2401 gcccgagagg atggagagcc ccacgacgtc ggcatcctcc tgtatggcgg tctcgacgat
     2461 ctgctccgcc gtctgatgga gaccggtgta gatgacctcc atgcccgcat cgcgcagcgc
     2521 ccgcgctatc accttcgcgc ccctgtcgtg gccatcgagc cccggctttg cgatgaccac
     2581 tcggatcttt ccgctcacgt cttccctccc gagggtgtct gccgaacggg cggcgctcct
     2641 cgcgacccgt tcgcgaccgt tgattctaga acacagccga cccctggtac accccgaaca
     2701 ccgacttcat ggtcgcgacc atctctccga gggtgcacat cgcccgagtg cactccagga
     2761 tcggaggcat gacgttcgtc ccgtccgcgc aggccgcccg cagatcttcc aggccacggg
     2821 cgaccgggct cgcgtcgcgt cgctcccgca gggcccgcag agagtcgact tgcctcatct
     2881 cgaactcctt gtcgatacgc agcgtctcga ccgcgggtcg cacaccggtc gcgaaccggt
     2941 tcacccccac cacgaccttg cgcccgctct ccaccgccat ctggtagcga tagctcgcgt
     3001 cggcgatctc ctgctggaag aagccctttg cgatggcggc cacaacaccg ccgagctctt
     3061 cgatcttgat gaagtactcc tcggcttccg cctccatgcg gtcggtgagg gactccacgt
     3121 agtaggaccc ggccaggggg tcgatgacgt tggccacacc gctctcgtac gccaacacct
     3181 gctgcgtgcg aagcgcgatc tccgccgccc gctcggtggg aagcgacaac acctcgtcca
     3241 gcgagttcgt gtgcagagac tgggtgccgc cgaggacccc ggccagcgct tcgatggtgg
     3301 tgcggacgac gttgttctcg ggctgctgtg ccgtcaggga acacccggcc gtctgcgtgt
     3361 ggaagcgcat cagccagctt cgtgggtcct tcgcacggta gcgctcgcgc atgtgccgcg
     3421 cccagatgcg acgggctgcc ctgaacttgg caacctcctc gaacaaatcg atgtgggcgt
     3481 tgaagaagaa ggagagtcgg ggggcgaagg agtcgacctc gagaccggcc gcgatgcccg
     3541 cctccacgta cgcgaatccg tcggccaggg tgaacgccaa ttcctgggcc gccgtagccc
     3601 ccgcctcgcg gatgtggtag cccgagatcg agatcgtgtt ccactgtggc acgtgctcac
     3661 cgcagaaagc gaagatgtcg gtgatcaggc gcagcgacgg ctcgggcggg aagatgtagg
     3721 tgttctgggc gatgtactcc ttgagtatgt cgttctggat ggtcccgccc acctgatcgt
     3781 gcttcacacc ctgccgctcg gctgccacca ggtagaaggc aagaaggatc gtagctgggg
     3841 cgttggtggt catggagacg gtcacctggt cgagagggat cccttcgaac acgcgctcca
     3901 tgtcgagaac ggagtccatg gctacgccgc acctgcccac ctcgccttcg gagcgcgggt
     3961 cgtcggagtc gcggcccatg agggtgggca gatcgagagc aagagagagt ccagtctggc
     4021 cttggtcgag caagaagcgg aaccggccgt tggtgtcttc ggcaccgccg aaaccggcga
     4081 actgacgcat ggtccagagg tggcccctgt acatggtggg gtggacgcca cgagtgtagg
     4141 gatactcacc tgggtagccg agttctcgat ggtagtcgtg tcccacccga tcgcccggat
     4201 cgtagatcgg ctccacctcc gcggaagaca ccgaggtgaa gtcggcgtcg cgcgtattct
     4261 gccaggagta tgctccggcg agccattcgg ccttcgttcg cgagccgtcg cgcacgtcat
     4321 ccacgtcgca ccctttcacc cgtccagttc caggagtccg gtcggtgggc ggctaccttc
     4381 ccagcgaagc ccggcttatg atgatccgct gcacttccga cgtcccctcg tagatctcgg
     4441 tgatcttggc gtcccggtag tagcgctcca ccgggaactc cttcgtgtat ccgtagccac
     4501 ccaagatctg caccgcctcg ccggtgtgct tcctcgccat ctcgctggcg aagagcttcg
     4561 ccctggcgcc cgcgacggtg gccgacgagc cgttcatctt caggcacgcc gccttgtgtg
     4621 tgagcaggcg ggccgcttcg aggtccttgt ccatgtcggc cagcttccag cggatcgcct
     4681 ggaagtccgc gatcctacgg ccgaactgac gccgctcctt cgcgtagcgc gaagccgcgt
     4741 cgaacgccgc ctgcgtgatg ccgaccgcct gcgccgcgat gccgatgcgc ccaccgtcca
     4801 gcgtcttcat ggcaatggag aaaccctcgc cttcggcgcc gagccgggcg gactcatgca
     4861 ccctgacgcc atcgaaggtg agagcg
//
LOCUS       DASRMJ010000208         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_418685, whole genome shotgun sequence.
ACCESSION   DASRMJ010000208 DASRMJ010000000
VERSION     DASRMJ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418685"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VFD74_05740"
     CDS             <1..923
                     /locus_tag="VFD74_05740"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49087.1"
                     /translation="DEAPETPEPLTVACAGVSQDENLVTRALLAIENATGWVFSGHVT
                     VRKNIPTGAGLGGGSSDGAAALRAGAEMVAEAGGPRLTPSQLHGLARGLGADVPFFLD
                     PMPALARGVGDLLEPLELPPLPLVLLLPESPLSTADVYRAFDGLAAGPEPAATSTSAA
                     AAPVASSDLAAGSGSAATPEPAAVPGPADTFEERFLAAERGWRGLSSAWSSGACGVEE
                     VVLEAARLLRNDLEEASLQLRPGLASTRAALDAEEVSGTLMSGSGPTLFGLCASSAVA
                     QAVAQRLCARGYDARAVVTGGAPAPRGPLP"
     gene            997..1068
                     /locus_tag="VFD74_05745"
     tRNA            997..1068
                     /locus_tag="VFD74_05745"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1029..1031,aa:Gln,seq:ttg)
     gene            1193..2650
                     /gene="glmU"
                     /locus_tag="VFD74_05750"
     CDS             1193..2650
                     /gene="glmU"
                     /locus_tag="VFD74_05750"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZK49088.1"
                     /translation="MSESIDTAVILAAGLGTRMRSTLPKVLHQVCGLPLITHVTRAAA
                     QAGVERLVVVLRPGQEEELAPHLPPSASVAVQKEQRGTGDALLAAGAMLGDGHVLVLP
                     GDTPLLTADILTGLVRLWSESGADAALLTLTPPDPTGYGRVLRDPEGMVERIVEHRDA
                     TPAEREVREVNAAVYILPAREALELLAGLDRDNDQGELYLTDVIAGLRSRGARIAALC
                     APDHRVALGINNRVELAEAQGIMGRRILEAWMLAGVTIEDPGSTHVDAGVELEPDVHL
                     LPFTCLRGTTRVARGSEIGPGSTLVDTVVGAGSFVRHSYTNGAVLEAGAQVGPFTYLR
                     PGANLLEGAKAGAFVEIKKSTIGKGSKVPHLSYIGDTRIGSGTNIGAGNITANYDGFE
                     KHPTSIGDNVRTGSDAVFVAPVTVGDEATVGAGSIITKDVPAGALAVARSRQRNILEY
                     RQKGRTHPSSEDAMRGGAAAEDHRVASDEQGGSDE"
     gene            2643..3641
                     /locus_tag="VFD74_05755"
     CDS             2643..3641
                     /locus_tag="VFD74_05755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZK49089.1"
                     /translation="MSDGSGRQASVLCATYRSLMLFSGRGNPELGAKIAEKLGIRLGS
                     VLTKTFADGEIYVKYGESVRGADVFIVQPTCRPVNENLMELLIMIQAARLASAHRITA
                     VVPWYAYSRQDKKSQPREPITGRLVADLLVAAGVDRVLTMDLHAGQLQGFFSIPVDHM
                     TAVPMLADYFKFKGLDDLVAVSPDAGGVKMAKRFGDRLGASLAVLTKLRPEHNEAKVM
                     HVIGDVAGKTAIIVDDIIDTAGTLCKGVEALLERGAKEVYAAATHPVLSGPAYERIAA
                     SNLKELVITDTIPLRTDVRRDKIVALSTAGILADTIHNVFADESVSALFQGENQLF"
     gene            3674..4324
                     /locus_tag="VFD74_05760"
     CDS             3674..4324
                     /locus_tag="VFD74_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZK49090.1"
                     /translation="MDTVKLTIHPREESGKGPCRRLRAAGLIPAVVYSKGQPSVSISI
                     AAADLKPALAGGSNVVLELHYADSKARKKHYAVVKDTQRLATRPRLLHLDLHEVDLKQ
                     EIEAPVAVELLGIPAGVDEGGVLEHSHREVVVRALPTSVPSNLELDVSALGIGDNLRV
                     SDLVAPDGVVILDDPETVLASVHAPRIEEVEAEEEAETFEAAEGEAAEPSAAGSTE"
     gene            4457..>4710
                     /locus_tag="VFD74_05765"
     CDS             4457..>4710
                     /locus_tag="VFD74_05765"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZK49091.1"
                     /translation="MFLVAALGNPGPQYQRHRHNVGFMVAEELRVRHGWPAFRTRYQG
                     LFGEGTMLGRRVGLLLPMTFMNRSGQGVAEAARFYRIPVEG"
BASE COUNT          742 a         1571 c         1609 g          788 t
ORIGIN      
        1 gggatgaagc tccagagacc cctgagccac tgacggtggc gtgcgcgggg gtctcccagg
       61 acgagaacct ggtgacccgg gcgctgttgg ccattgagaa cgcgaccggg tgggtcttca
      121 gcggccacgt caccgtgcgc aagaacatcc ccacgggcgc cggcttgggc ggcggcagct
      181 cggacggcgc ggcggcgctc cgcgccggcg ccgagatggt ggcggaagcg ggtggcccga
      241 ggttgacgcc gtcgcaactg cacggtctgg ctcgcggtct gggcgccgac gtgcccttct
      301 tcctcgatcc gatgcccgct ctcgcccgtg gcgtcggcga tctgctggag ccgttggagc
      361 tgccgcccct gccgctggtc ctgctgctgc cggagagccc gctctccaca gcggacgtct
      421 accgtgcctt cgacggcctc gccgccggcc ccgagcccgc cgccacatcc acgtccgccg
      481 ccgcggcgcc cgtcgcctcc tccgaccttg ccgccggctc cgggtccgcc gccactcccg
      541 agcccgccgc cgtccccgga cctgccgaca ccttcgagga gcgtttcctt gccgctgagc
      601 ggggctggag aggtctctct tcggcctggt cgtcgggtgc ctgtggtgtc gaggaggtgg
      661 tcctcgaggc ggcacggctg ctccgcaacg acctggagga ggcgagcctc cagctgcgcc
      721 ccggactcgc ctctacccgc gccgccctcg atgcggagga ggtgtcgggc actctcatga
      781 gcgggtccgg gcccacactc ttcggcctgt gtgcgtcatc ggcggtggcg caggcggtgg
      841 cgcagaggct ttgcgcgcgc ggctacgacg cgcgcgccgt cgtgaccggg ggagcgccgg
      901 ctccgcgtgg tccgcttcct tgacaccttc gggtgccctg cctatacttc ccgggccggc
      961 gcgggccttg acgcttcatg cattgcgcgg gttcgctggg gcgtagccaa gtggtaaggc
     1021 aacgggtttt ggtcccgtca ttcgtaggtt cgaatcctgc cgccccagtt tcctgatttc
     1081 tggtccattc ttccctgcgt ggtgttcgac ggcggcggga gtctggtagg ctccatgtcg
     1141 gcaccttcgg gggccgtgcg tcgagggatc ctcatcgggg gcgcgcatca tcgtgagtga
     1201 atcgatcgac accgcggtca ttcttgcggc cgggctcggc acgcggatgc ggtcgaccct
     1261 acccaaagtc ctgcaccagg tgtgcggtct tcctctgatc acccacgtca cgcgcgccgc
     1321 ggcacaggcc ggcgtcgaac gtctcgtcgt ggtgctgcgg ccggggcagg aagaggagct
     1381 ggcgccccat ctgccgccgt cggcatcggt cgcggtccag aaggagcaac gtggcacagg
     1441 tgacgccctg ctcgcggcgg gcgcgatgct cggtgacggc cacgtgctcg tgctgccggg
     1501 cgacacgccg cttctcaccg ccgacatcct gaccggtctc gtgcggctct ggtccgaatc
     1561 gggggccgac gcggctctgc tcactctgac gccacccgat cccaccggct acggccgcgt
     1621 gctccgcgat cccgagggca tggtggagcg catcgtcgag caccgtgacg ccaccccggc
     1681 tgagcgggag gtgcgtgagg tgaacgccgc cgtctacatc cttccggccc gcgaagcgct
     1741 cgagcttctg gctgggctcg accgcgacaa cgaccagggg gagctgtacc tcaccgatgt
     1801 gatagccggt ctgcggagtc gcggggcgcg gatcgcagct ctgtgcgccc cggatcaccg
     1861 cgtcgctctt ggcatcaaca accgggtgga gttggccgag gcccagggca tcatgggccg
     1921 gcgcatcctg gaggcgtgga tgctggccgg cgtcaccata gaggatcccg gctccaccca
     1981 cgtggacgcc ggcgtcgaat tggagcccga cgtgcaccta ctccccttca cgtgtctgcg
     2041 ggggaccacc cgcgtggcgc ggggcagtga gatcggaccg ggctccactc tggtggacac
     2101 ggtcgtcggg gccgggagct tcgtgcgcca ctcctacacc aacggcgccg tgcttgaggc
     2161 gggtgcccag gttgggccct tcacctatct gcgacccggc gcgaaccttc tggagggcgc
     2221 caaggccggg gccttcgtgg agatcaagaa gagcaccatc ggcaagggca gcaaggtgcc
     2281 tcacctgagc tacatcggcg acacccgaat cggttccggc accaatatcg gggcggggaa
     2341 catcacggcc aattacgatg gcttcgagaa gcaccccacc tccatcggtg ataacgtgag
     2401 gacgggttcc gacgcggtct tcgtggcccc ggtcacggtc ggcgacgaag ccactgtcgg
     2461 ggcgggatct atcatcacca aggacgtccc ggctggagct ctggcggtcg cgcgcagcag
     2521 acaaaggaac attcttgagt atagacaaaa gggtcgaacg catccgtctt ccgaggacgc
     2581 aatgagggga ggggcggcgg cggaagacca ccgcgtcgcc tccgacgaac agggaggatc
     2641 agatgagtga cgggtccggg cgccaggcct ccgtcctatg cgcgacgtac cgttctctga
     2701 tgttgttctc gggccgtggc aacccggagc tgggcgcgaa gatcgccgag aagctgggga
     2761 tccgcctggg atccgttctg acaaagacct tcgccgacgg cgagatctac gtcaagtatg
     2821 gggagtctgt ccgaggtgcc gacgttttca tcgtgcagcc cacctgtcgg ccggtgaacg
     2881 agaacctgat ggagctgctc atcatgatcc aggccgccag actcgcctcg gcccaccgga
     2941 tcacggccgt ggtgccgtgg tacgcctact cccgccaaga caagaagagc cagccgcggg
     3001 agcccatcac cggccgcttg gtggccgatc tcctggtggc cgcaggcgtc gaccgcgtcc
     3061 tgaccatgga cctccacgcc ggacaactcc aaggcttctt cagcatcccc gtggaccaca
     3121 tgaccgccgt gcccatgctg gccgattact tcaagttcaa agggctcgac gatctggtgg
     3181 cggtctcgcc cgacgccggg ggtgttaaga tggccaagcg cttcggggat cgcctgggag
     3241 cgtcgctcgc cgtgctcacg aagttgaggc ccgagcacaa cgaggcgaaa gtcatgcacg
     3301 tcatcggtga cgtcgccggg aagacggcca tcatcgtcga cgacataatc gacacggctg
     3361 ggaccctctg caaaggggtg gaagcgctgc tcgagagagg cgcgaaggag gtctacgcag
     3421 ctgcgacgca ccccgtgctt tcgggcccgg cgtacgagcg catagcggcg tcgaacttga
     3481 aggaactcgt catcaccgac actatccccc tgcggaccga tgtgcggcgc gacaagatcg
     3541 tagcgctgtc taccgccggg atattggccg acactatcca caacgtcttc gccgacgagt
     3601 ctgtgagcgc cctcttccag ggcgagaacc agctcttcta gcacaggcga gactcaagta
     3661 tcagggagag attatggaca ccgtcaaact caccatccac ccgcgggagg agtccggcaa
     3721 aggaccctgc cgccgtctgc gcgccgccgg cttgatcccg gcggtcgtgt actcgaaggg
     3781 ccagccgagc gtctccatca gcatcgcggc ggccgacttg aagccggccc tcgcgggcgg
     3841 ttcgaacgtc gtgctggagt tgcactacgc agacagcaag gcccgcaaga agcactacgc
     3901 agtggtcaag gacacgcagc gcctcgccac tcgccctcgg ctgctgcacc tcgacctgca
     3961 cgaagtcgat ctgaagcagg agatcgaggc ccccgtcgcc gtcgagttgt tggggatacc
     4021 ggccggcgtg gacgaaggcg gagtgctcga gcactcccat cgtgaggtcg tggtgcgggc
     4081 actgccgact tcggtgccgt ctaatctgga actcgacgtg agcgccctgg gcatcggcga
     4141 caacctgcgg gtgtccgatc tggtggctcc cgacggcgtc gtcatcctcg acgatcctga
     4201 gaccgtcctg gcttcggtgc acgcgccgcg gattgaagaa gtggaggcgg aagaggaagc
     4261 cgagacgttc gaagcagcgg aaggtgaagc ggccgagccg tccgcggcag gctcgactga
     4321 gtagtagtct cggtccgcac gcagaagatg cgaggacgcc gagctgtgcc tcccggcgcg
     4381 gccgcggcgt cctcgacgtt ccgggattcg tgttgagcga gtccctgacc ccggcagctc
     4441 ttcgaggtag ttgaacatgt tcttggtcgc ggccctcggc aatccggggc cccaatatca
     4501 gcggcaccgt cacaacgtgg gcttcatggt ggctgaagag ctacgtgtgc gtcacgggtg
     4561 gccggccttc cgcacccgct atcaggggct gttcggtgaa ggcaccatgc tcgggcgccg
     4621 cgtggggctg ctgctgccca tgacgttcat gaaccgctcc gggcagggcg tcgccgaagc
     4681 cgcccgcttc taccgcatcc cggtggaagg
//
LOCUS       DASRMJ010000209         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_523029, whole genome shotgun sequence.
ACCESSION   DASRMJ010000209 DASRMJ010000000
VERSION     DASRMJ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523029"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFD74_05770"
     CDS             <1..201
                     /locus_tag="VFD74_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49092.1"
                     /translation="LWELVGEQFPLQGAVEDVDAVCPVCHVRAHVGPGAETGNAFACG
                     LCGAELTVDASSGTPSLVAAEA"
     gene            complement(243..1307)
                     /locus_tag="VFD74_05775"
     CDS             complement(243..1307)
                     /locus_tag="VFD74_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZK49093.1"
                     /translation="MANQNISRVILTLFVTGALLGLAGGAFLGGDRVPSAPTDSTSPA
                     GAAAAPTGPMTSARAAAMGANELGRILVLEYHQIGAPEADWTRTPEQFRADIDLLKSE
                     GFYPIDVRDLATGNIEVAAGKTPVVLTFDDSSPGQYRILDDQTIDPDSAVGIMEAAVR
                     SGGWASRASFYVLLDVRPDDHVLFGQPDYQTKKLRSLVEWGYEVGSHTVSHLNLKEAY
                     AEESTKQLALSAKMIEDMVGSGYKVQTLSIPLGEYPADDAILRSGTWESIAYGYIAAL
                     KVSGGASPSPFSQEFDPLHIPRVIVRGNALREAIQAFKDEPGLRYVSDGDPAAVSVPA
                     ETAEALGTLRADLGRPVITY"
     gene            1415..2653
                     /locus_tag="VFD74_05780"
     CDS             1415..2653
                     /locus_tag="VFD74_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HZK49094.1"
                     /translation="MGEYFSPPRRRARLYAGPVSVLVIVAGLAAFAVGFVLFWGVGHR
                     LAGGEFEFALPRFGSETPRVEEVAKGLATPIEYPTQVTFDPVAYRDAHYVPVKGIYIS
                     SWIAGSPDLFAQQISLVDRTELNAMVIDVKDATGYVSYDSQVALADELDLEEHRIKDI
                     GAVVAALNEHHIVPIARIVCFNDKLLSARRPEWGVQHKNGGLWHDKDGNSYTNAYDRR
                     VWEYLVQLAEDAASRGFREIQFDYVRFPSDGKISDAVYPGRSGPTEDAIAEFLAFARQ
                     RLDKRGVWVSADVFGLTVHVRDDLGIGQKIEKVAANVDIVSPMIYPSHYRAGSYGIAA
                     PNSSPYEVITKAMADSGARLSGSGAIVRPWLQDFSLYGVTYGETEVRAQIKAVEDQGF
                     MEWILWDPSVKYVEAALRPG"
BASE COUNT          482 a          856 c          974 g          489 t
ORIGIN      
        1 ctgtgggagc tcgtcggtga gcagttcccg cttcaaggcg cggtggaaga cgtagacgcg
       61 gtgtgcccgg tctgtcacgt gcgcgcgcac gtggggcccg gagccgagac ggggaacgcc
      121 ttcgcctgcg gcctctgcgg cgccgagctg acggtcgacg cctcgtcggg cacgccgagc
      181 ctggtagcgg ccgaggcata gcgagccgcc gggggcgacg atgctgtggc gctcggaggc
      241 agctagtagg tgatgacggg ccgaccaagg tcggcccgta gcgtaccgag tgcttccgcc
      301 gtctcagccg gcaccgatac cgccgcggga tcgccgtcag agacgtagcg cagaccgggc
      361 tcatccttga acgcctggat ggcctcgcgg agcgcgttcc ccctgacgat cactcggggg
      421 atgtggagtg ggtcgaactc ctgcgagaag ggcgacggag aagcgccgcc cgagaccttt
      481 agggctgcga tgtagccgta ggctatggac tcccacgtgc cgctccgaag gatggcgtca
      541 tccgccgggt actcgcccag cgggatggag agggtctgca ccttgtaacc cgatccgacc
      601 atatcctcga tcatcttcgc ggagagcgcc agctgtttcg tgctctcctc ggcgtaggct
      661 tctttgaggt tgaggtgact cacggtgtgg ctgcccacct cgtaccccca ctccacaagg
      721 cttcgcagct tcttcgtctg gtagtcgggc tggccgaaca gcacgtggtc gtcgggccgc
      781 acgtcgagca gcacgtagaa cgaggcgcga gaggcccagc cgcccgaccg gaccgccgcc
      841 tccatgatgc ccaccgcgct gtcggggtcg atggtctggt cgtcaaggat gcggtactgg
      901 cccgggctgg agtcgtcgaa ggtgagcact accggtgtct tgccggcggc aacctcgatg
      961 ttgccggtgg ccaggtcgcg cacgtcgatc ggatagaagc cttcggactt gagcaggtcg
     1021 atatccgctc ggaattgctc aggggtgcgg gtccagtcgg cctccggggc cccgatctgg
     1081 tggtattcga gcacgaggat ccttccaagt tcgttcgcgc ccattgcggc tgcgcgtgcg
     1141 gaggtcatgg ggccggtggg cgctgctgcc gcgccggcgg gcgatgtgga gtccgtgggc
     1201 gcggacggaa cacggtcacc tccgaggaag gcgccgccgg cgagccccag aagggctccg
     1261 gtcacgaaaa gggtgaggat cacccgggat atgttctggt tggccatggg gatgcaaagt
     1321 agcagaaacg agaccgactg accacgcggg cgccacgcgc ggggtgggtg tcaccggccc
     1381 cggatgaagt cgattctgct agactaccgg tcctatgggt gagtacttca gccctccgcg
     1441 caggcgcgca cgactctacg ccggccccgt cagcgtgctg gtgatagtgg ccgggctcgc
     1501 agcctttgcg gtgggctttg ttctcttctg gggtgtgggc catcgacttg cgggcggtga
     1561 gttcgagttc gctcttccgc gcttcggaag tgagactccg cgggtggagg aagtggcgaa
     1621 gggtctcgcc acgccgatcg aatatcccac gcaggtgacg ttcgatcccg tagcctaccg
     1681 agacgcacac tacgtccccg tcaaaggcat ctacatctcg agttggatcg cagggtcgcc
     1741 cgatctcttc gcgcagcaga tctccctcgt ggaccgcacc gagctcaacg ccatggttat
     1801 cgacgtcaaa gacgccacgg ggtacgtctc gtacgactcc caggtggccc tcgcggacga
     1861 actcgatctt gaggagcatc ggatcaaaga tatcggggcc gtggtggccg cgttgaacga
     1921 gcaccacatc gtcccgatcg cccgcatcgt ctgcttcaac gacaagctcc tctctgcgcg
     1981 tcgcccggag tggggggtgc agcacaagaa cggcggcctc tggcacgaca aggacgggaa
     2041 ttcctacacg aacgcgtacg atcggcgagt gtgggagtac ttggtgcagt tggcggagga
     2101 cgccgcctcg cgaggcttcc gcgagatcca attcgactac gtgcgcttcc cgagcgacgg
     2161 gaagatctcg gatgcggtgt atccggggcg gagcggcccg acggaggatg cgatcgccga
     2221 gttcctggcg ttcgccagac agcggctcga taagcggggc gtgtgggtgt cggccgacgt
     2281 gttcggcctg acggtccacg tgagagacga cctcggcatt ggtcagaaga tagagaaggt
     2341 ggccgccaat gtggacatcg tgagcccgat gatctacccg tcgcactaca gggcggggtc
     2401 gtacggcatc gccgccccga actcctcgcc gtatgaagtc atcaccaaag caatggcaga
     2461 ctccggcgct cgactcagcg gcagcggagc gatcgtgcgg ccttggctgc aggacttctc
     2521 gctctacggc gtcacctatg gagagaccga ggtgagagcc cagatcaagg cagtcgagga
     2581 ccaggggttc atggagtgga tcctctggga tccatcggtg aagtacgtag aagcggcgct
     2641 tcgccccgga tagcggtgac agggaatcgc ctcgttcgcg ccggcggcac gatccgtccg
     2701 atcagccctg atcgagctcc aggacgaggg ccacctcgtc gcaatcagga aagtgcacgc
     2761 agtggacaca ctcgcgccag atcttgtgag ggagctccga c
//
LOCUS       DASRMJ010000210         3994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_197882, whole genome shotgun sequence.
ACCESSION   DASRMJ010000210 DASRMJ010000000
VERSION     DASRMJ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197882"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1017)
                     /gene="argB"
                     /locus_tag="VFD74_05785"
     CDS             complement(112..1017)
                     /gene="argB"
                     /locus_tag="VFD74_05785"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015134389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZK49095.1"
                     /translation="MREAYRKRVETLLEALPYIRQFSGKTVVIKYGGAAMSSADLKDE
                     FATDIVLLRYVGIRPIIVHGGGNEVSGLMDRLDLPVRFVDGLRVTDKAAMEVAKMVLV
                     GKVNKEIVSLINQRGAQAVGLGGDDGLLLQARKLERRGSDGSPVELGFVGEVHTVNTS
                     LLRLLEDDYVPVVASVGAGPDGESFNINADTVAAAVAAALQAEKIVFLTDVAGLYSDF
                     DDQNSLISECSADEVRALMGSGRVSRGMIPKLEAVLTALDAGVRSAHIIDGRVAHSIL
                     LEILTEDSGVGTMVRRSAGSDGGEA"
     gene            complement(1030..2334)
                     /gene="argJ"
                     /locus_tag="VFD74_05790"
     CDS             complement(1030..2334)
                     /gene="argJ"
                     /locus_tag="VFD74_05790"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZK49096.1"
                     /translation="MSETAGPGHPLVVDPRSSLFLSLPAKVTEVHEAEEVGVTFPRGF
                     VAAGVAAGIKESGRPDVGVVAVEHQYRTASVGAAVFTRNSFAAAPVVVSRKETALPRL
                     AAVAMNSGNANACTGAEGLAVARAMQSACATGLELPVERVGVASTGIIGVPLDQERLT
                     AGVRGAVAELSRTGGARFAAAIMTTDRFDKAVALEFDTPNGAVRVGACAKGAGMIAPG
                     MATMLCVVTTDALVPPTVASSLLSREVGHTFNRVTVDGEMSTNDSVFFLASGASGVEL
                     VAEGVGRLGSALRYALLRLALMMVADGEGATKVMRLRVDGAPDDEDANLAARAVANST
                     LVKTAMYGCDPNWGRILSAAGAALPARSFPSVTLRIGGVTLVERATAVSLEPEEAARL
                     REVMHGSEVDVVLDFKSGVGTAEVYFADLGHEYVTINAEYHT"
     gene            complement(2331..3368)
                     /gene="argC"
                     /locus_tag="VFD74_05795"
     CDS             complement(2331..3368)
                     /gene="argC"
                     /locus_tag="VFD74_05795"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZK49097.1"
                     /translation="MITASIVGATGFTGMVLTDLLDRHPEVRLDALTSVSYAGRTVPD
                     LFPQLRVRGAYVEYAAERVADSEYVFVCYPHAAAHPVVAELLDAGHKVVDLSADFRLS
                     EPDAYPRWYGFEHPRVDLLGEAVYGIPEVYRDAVAHARLVANPGCFPTVSLLAALPLL
                     RLGSVGRVIVDAKSGVSGAGRSPSRKTHFCTVHDDFRAYGELGHRHTSEMLQEMGAAA
                     GRSLPVSFTPHLLPVDRGILATVYCIPGVVAATGAVAPLLSQDELEEMYVSAYEGEPF
                     VEVRRDVPSLGEVQHTNFCRMAVRVDADAGLYKIVGVIDNLVKGASGQAVQNMNIMAG
                     IPETMGLGARA"
     gene            complement(3524..>3994)
                     /locus_tag="VFD74_05800"
     CDS             complement(3524..>3994)
                     /locus_tag="VFD74_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZK49098.1"
                     /translation="TVGVDSARPSDPAGPVALGWVGEVHPLVLKAFGLPGTALAAELD
                     LDALLSARRVLPLFEDLLTHPALTQDLALVVGLDVPAIDLVAAVKRAGDELLQDVQVF
                     DVYVGPQVGEGRKSLALRLTYRSSERTLQETEVNELRAAMLEELRVSIGAELRT"
BASE COUNT          711 a         1430 c         1227 g          626 t
ORIGIN      
        1 cctccagagg caggcgcgcg taggtgggca ggacatattc gcgatccaga gccgcgatat
       61 ccggggcgat gcgcggcacg gtcgccccaa tgcccccgcc gggtacggcg ctcatgcctc
      121 ccccccgtcg cttccggccg atcgcctgac catagtgccg acgccggagt cctcagtgag
      181 tatctcgagc aggatcgagt gggcgacccg gccgtcgatg atgtgcgcgg aacgcacgcc
      241 ggcatcgagg gcggtcagaa ctgcctcaag cttggggatc atcccccgcg acacgcgacc
      301 agatcccatc aacgcccgca cctcgtcggc gctgcactcc gagatcagac tgttctggtc
      361 gtcgaagtcg gagtataggc ccgcaacgtc cgtcaagaac acgatcttct ccgcctgcag
      421 ggcggccgca acagcggcgg ccacggtatc cgcgtttatg ttgaagctct caccatcggg
      481 gccggctccc acggaggcca ccaccggcac gtagtcgtcc tcgagcagtc ggaggaggct
      541 cgtgttgacc gtgtgcacct ctcccacgaa gccgagttcc accggcgatc catcggagcc
      601 gcggcgctcg agcttccgtg cctgcagaag gagtccgtca tctcctccga ggcccacagc
      661 ctgcgcccca cgctgattga tcaggctgac tatctccttg ttgaccttcc ccacgagcac
      721 catcttggcc acctccatgg cggccttgtc ggtgacccgg agaccgtcca cgaaccgcac
      781 cggcaggtcg agcctgtcca tgagacccga cacctcgttg cccccgccgt gcacgattat
      841 cgggcggatg cccacgtagc gcagaaggac tatgtcggtg gcgaactcgt ccttgaggtc
      901 ggcggagctc atcgccgccc cgccgtactt gatcaccacc gtctttccgc tgaattgccg
      961 gatgtacgga agggcttcga gaagggtctc cacccgtttc cggtacgctt cacgcatcgg
     1021 ccgagcctcc taggtgtggt attcggcgtt gatcgtgacg tactcgtggc cgaggtcggc
     1081 gaagtagacc tcggcggtgc ccactccgct cttaaagtcg agcacgacgt cgacctcgct
     1141 cccgtgcatg acctctcgca gccgagcggc ctcctccggc tccagggaaa cggccgtcgc
     1201 gcgctccacc agggtcaccc cgcctatacg cagcgtcacg ctcgggaacg agcgggcggg
     1261 caatgcggct cccgcggcgc tcagtatgcg cccccagttg gggtcgcacc catacatggc
     1321 cgtcttgacc aaggtcgagt tcgcgacggc cctggcggcg aggttggcgt cttcgtcgtc
     1381 gggcgcgccg tcgacccgga gacgcatcac cttggtggcg ccctcgccgt cggcgaccat
     1441 catcaaagcc agacgaagga gcgcgtagcg cagggccgac ccaagacgac ccactccttc
     1501 ggcgaccaac tcgactccgc tcgcgccgct cgccaggaag aagacggagt cgttggtgct
     1561 catctcgccg tccacggtga cgcggttgaa ggtgtgcccc acctctctcg agagcagact
     1621 cgaggccacc gtgggcggca ccagagcatc cgtggtgacc acgcagagca tggtggccat
     1681 cccgggcgcg atcatccccg cgcccttcgc gcacgctccc acacgcacgg ccccgttggg
     1741 agtatcgaac tccaaggcaa ccgccttgtc gaagcggtcg gtggtcatga tggccgcggc
     1801 gaagcgcgcg cctccagtgc ggctcagttc cgcgactgcc cctcgcaccc cagccgtcag
     1861 cctctcctgg tcgaggggta cgccgatgat gcccgtggaa gcgacaccga ccctctcgac
     1921 gggaagctcc aggccggtcg cgcacgcgct ctgcatggcc ctagccaccg cgagcccctc
     1981 cgccccggtg cacgcgttgg cgttgccgct gttcatggcc acggcggcca atctcggaag
     2041 cgcggtctcc tttcgactca ccaccaccgg ggccgccgcg aaggagttgc gcgtgaacac
     2101 cgcggccccc accgaagccg tgcggtactg atgctcgacg gcgacgacgc ccacgtctgg
     2161 ccggccgctc tccttgatcc cggcggccac cccggccgcc acgaaaccgc ggggaaaggt
     2221 gacgccgacc tcttccgctt cgtgcacctc agtcaccttc gcggggagac tgaggaacag
     2281 tgacgaccgc ggatccacca cgaggggatg accaggacct gccgtctcac tcatgcgcgc
     2341 gcccccagtc ccatggtctc cggaatcccc gccatgatgt tcatgttctg caccgcctgt
     2401 cctgaggcac ccttgaccag attgtcgatc acgccgacga tcttgtagag gccggcatcc
     2461 gcgtccaccc gcacggccat ccgacagaag ttggtgtgct gcacctcccc aaggctcggc
     2521 acgtcccggc gcacctccac gaacggctca ccttcgtagg ccgagacgta catctcctcg
     2581 agttcgtcct ggctcaggag aggtgcgacg gccccggtgg ccgcaacgac gccgggaatg
     2641 cagtacaccg tggcaagtat ccctcggtcg acaggaagaa gatgaggagt gaacgacacc
     2701 ggcagactgc gcccggcggc ggctcccatc tcctggagca tctctgaggt gtgccgatga
     2761 cccagctcgc cgtaggcccg aaagtcgtcg tgcaccgtgc agaagtgcgt cttccgagac
     2821 ggcgaccgac cggccccaga gacacccgac ttggcatcga cgatgacgcg cccgaccgat
     2881 cccaggcgca acaaaggcag cgccgcaagc agggagacgg tggggaagca gccgggattg
     2941 gccacaagcc tcgcgtgggc gacggcatcc cgatagacct cggggatgcc gtaaacggcc
     3001 tccccgagca ggtcgacccg cggatgttcg aatccgtacc agcgaggata tgcatcgggc
     3061 tcactcaagc ggaagtctgc gctgaggtcg acgactttgt gaccggcgtc gagcagttcc
     3121 gccaccaccg gatgggcggc cgcatgcgga tagcacacga acacatactc agagtcggcg
     3181 acccgctcag ccgcgtactc gacgtaggca ccgcgcaccc gcagctgtgg gaagagatcg
     3241 ggcacggtcc gacccgcgta tgagacggag gtgagcgcgt ccaggcgtac ctcggggtgc
     3301 cgatcgagga ggtcggtcag caccatccct gtgaacccgg tggcccccac gatgcttgca
     3361 gtgatcacgc ccactccccg cgtcgaaccc agatacctac gaacctgact gcataagtat
     3421 accgttgcat gcaaatacat gcaaccgaat atggccgcca accgcatccg cctggtctac
     3481 gatcgccggc gggccggtcg cccccggcta cgtcaaagtc cggctaagta cgcaactccg
     3541 caccaatcga tacccggagc tcttcgagca tcgcggctcg caactcgttg acctcggtct
     3601 cctgaagcgt gcgctccgac gaccggtagg tgagtcggag ggccaggctc tttcgaccct
     3661 cgcccacctg cggccccacg tagacgtcga acacctgcac gtcctgcagg agctcatcac
     3721 ccgcccgttt gactgcggcc accaggtcga tagctggcac gtcgagccca acaaccagcg
     3781 ccaggtcttg tgtcaaagcc ggatgcgtga ggaggtcttc gaagagcggc aagacccggc
     3841 gcgctgacaa caacgcatcg aggtcgagct ccgccgcgag ggcggtcccg gggagcccga
     3901 acgccttcag caccagcggg tggacctcgc ccacccagcc tagagccacc ggcccagcgg
     3961 gatcactcgg tcgtgccgag tcgacgccga cggt
//
LOCUS       DASRMJ010000211         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_305477, whole genome shotgun sequence.
ACCESSION   DASRMJ010000211 DASRMJ010000000
VERSION     DASRMJ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305477"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFD74_05805"
     CDS             <1..265
                     /locus_tag="VFD74_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49099.1"
                     /translation="YHLASSRSQLWLSERASSTAALLSVAAFLGRITIAGLVLVALHF
                     LTPLDVVAVAVAFVALFSILSGYALYRFAKKGSGPGISSQVLP"
     gene            278..1072
                     /gene="atpB"
                     /locus_tag="VFD74_05810"
     CDS             278..1072
                     /gene="atpB"
                     /locus_tag="VFD74_05810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZK49100.1"
                     /translation="MSTEAEASKVTEALHELNVQQVIQLPKLGPIDLSITNAVLYMWL
                     SALLVFVFFFVISRRLKKEPDTLQTIGEILLNFATGHLTGQIGEKGKKYYYLILTIFA
                     YIFTMNILGLVPKPEFLKPYTPTANINVTFGMAFVVFLVVQFQGFRHHGFRGYLGSWL
                     TPPGVPKALAIPLNLVFFVVHLMGELFKPLSLAIRLFGNTIAGHLVILVMLGLVIEFQ
                     SYLIAIPPLAFVLAMNGFEIFVAFIQAYIFALLSVIYIESAIYAGH"
     gene            1100..1330
                     /gene="atpE"
                     /locus_tag="VFD74_05815"
     CDS             1100..1330
                     /gene="atpE"
                     /locus_tag="VFD74_05815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HZK49101.1"
                     /translation="MDAETAAKLAAGLGMGLATLGPGLGIGYMTGRTADAIARQPEAS
                     GDIRTAMIIGVALAEALALYAFVIAILLAVAS"
     gene            1402..1908
                     /gene="atpF"
                     /locus_tag="VFD74_05820"
     CDS             1402..1908
                     /gene="atpF"
                     /locus_tag="VFD74_05820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZK49102.1"
                     /translation="MKIFEIDPGLFIWSMLTFILLVLLLYKFAFGPLMALQKQRQDEI
                     HSSIRESEGLRDEAHALLEDYKKQLASARQESEDILERARKIGENTKGEILDEAKAQA
                     EWAVEKAHEQIERETRQALQEIKAEVAALTLLATEKVTRKTLTAQDHLDLINEAIAEI
                     DLGKFSEN"
     gene            1921..2493
                     /gene="atpH"
                     /locus_tag="VFD74_05825"
     CDS             1921..2493
                     /gene="atpH"
                     /locus_tag="VFD74_05825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HZK49103.1"
                     /translation="MSQTRIARVYAGALYEAAVDAGKVDEVRGDLTAFVDALAQSAEL
                     RAFLLNEQLPGEEKKQALMQMTEGGEALVRNFLRVLVDKHRESVLEEASVVFVDRAEK
                     DAGVVKVELATAVPVLDAMRESLVKSLETSLGKRVELTLTVDESVLGGVKLRIGDRIA
                     DASLRHRLEGLRARLVSPTARLEGSVEAAS"
     gene            2477..>3669
                     /gene="atpA"
                     /locus_tag="VFD74_05830"
     CDS             2477..>3669
                     /gene="atpA"
                     /locus_tag="VFD74_05830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZK49104.1"
                     /translation="MKLRPEEITAVLKSRIKDYEAEVDMQEVGTVVMTGDGIARIHGL
                     ENAVASEMLELPHGVTGLVLNLEEDSVGAVLLGESTLIKEGDLVKRTGKVMQVPVGDA
                     LVGRVVSPLGIPLDDKGPIETTEFRAVEFKAPGVIRRQPVTEPLQTGLKAIDSMIPIG
                     RGQRELIIGDRGTGKTAIAVDTIINQKGQDVICVYVAIGQKASTVRQVLAKLEDHGAM
                     EYTIIVMAGASEAAPLRYIAPYAGCAMAEFYTYQGKAALCIYDDLTKQADAYRQMSLL
                     LRRPPGREAFPGDVFYLHSRLLERACKLSDKEGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESNLFYGGVRPAINVGISVSRVGGDAQIKAMKKVAGSLRLDLSQYREL
                     EAFAK"
BASE COUNT          671 a         1162 c         1157 g          679 t
ORIGIN      
        1 gtaccatctc gcctcgtctc gatcgcagtt gtggctatcg gagcgtgcga gcagcactgc
       61 ggcgctcctc tctgtggcag ccttccttgg ccgcatcacc atcgcgggcc tcgtgctcgt
      121 ggctcttcac ttcctcacgc ccctcgacgt cgtggccgtg gccgtggcgt tcgtcgccct
      181 cttcagcata ctgagcggct acgcgctgta ccgcttcgcg aagaagggca gtggcccagg
      241 tatctcctcg caagtcttgc cttagaagga gtgttgcgtg tctacggaag ctgaagcgag
      301 caaggtcaca gaggctcttc acgagctgaa cgtgcagcag gtcatccagc tgcccaagct
      361 cgggccgatc gatctctcga tcacgaacgc cgtgctctac atgtggctct cggcgctcct
      421 ggttttcgtg ttcttcttcg tcatcagccg gcgcctgaag aaggaacccg acactctgca
      481 gaccatcggg gagatactcc tgaacttcgc caccggccat ctcactggtc agataggcga
      541 gaagggtaag aagtactact acctcattct tacgatcttc gcctatatct tcacgatgaa
      601 catcctcggc ttggtgccca agcctgagtt cctgaagccc tacacgccta ccgcgaacat
      661 caacgtcact ttcgggatgg cgttcgtcgt gttcctggtg gtgcagttcc agggcttccg
      721 tcatcacggg ttccgcggat acctcggctc ctggctcacc cccccgggtg tgcccaaagc
      781 cctggctatc ccgctgaacc tcgtcttctt cgtggttcac ctgatgggcg agctcttcaa
      841 gcccctctcg ctagccatcc ggttgttcgg caacacgatc gcgggtcacc tggtcatcct
      901 cgtgatgctg ggcctcgtga tcgagttcca gagctacctg atcgcaatcc cccccctcgc
      961 gttcgtgctg gcgatgaacg ggttcgagat ctttgtagcc ttcatacagg cctacatctt
     1021 cgcgctcctc tcggtgatct acatagagtc cgcgatctac gcgggccact aaacaatgct
     1081 acgaaaggag tagtaccaag tggatgctga aaccgcagcc aaacttgccg ccggtcttgg
     1141 gatgggtctt gccaccctcg gccccggtct gggcatcggc tacatgaccg gtcgcaccgc
     1201 cgacgccatc gctcggcagc cggaagcctc cggtgacatc cgtaccgcca tgatcattgg
     1261 tgtggccctg gccgaagcgc tcgcgctgta tgccttcgtc atcgccatcc tcctagcggt
     1321 cgccagctag gacacacgat tgggcgccgg gggccgcgag gcccccggcg cgatcaccac
     1381 tgaggagagc ttgagccgag catgaagatc tttgagatag acccggggct gttcatctgg
     1441 tcgatgctga ccttcatcct tctggtcctc cttctgtaca agttcgcctt cgggcctctg
     1501 atggcgctcc agaagcagcg tcaggacgag atccactcgt cgatccggga gtcggaaggt
     1561 ctgcgcgacg aagctcacgc gctgctggag gactacaaga agcagctcgc gtccgcccgg
     1621 caggagagcg aagacatcct tgaacgggcc cgcaagatag gcgagaacac caaaggggag
     1681 atcctggacg aggccaaagc ccaggcggag tgggcggttg agaaggctca cgagcagatc
     1741 gagcgcgaga cccgccaggc gctgcaggag atcaaggcgg aggtcgcggc gttgaccctg
     1801 ctggccaccg agaaggtcac caggaagacc ctcaccgcac aagaccacct ggacctgatc
     1861 aacgaggcga tcgccgagat cgacctgggc aagttcagcg agaactagga agagagaccg
     1921 gtgagtcaga ccaggatcgc cagagtttac gccggggcgc tgtacgaagc ggccgtcgac
     1981 gcggggaagg tcgacgaggt ccgcggggac ctgaccgctt tcgtggatgc cctcgcgcag
     2041 tccgcagagt tgcgggcctt tctgctgaac gagcagctcc ccggcgagga gaagaagcag
     2101 gcccttatgc agatgacgga ggggggagag gccctggtgc gcaacttcct gcgcgtgctc
     2161 gtggacaagc accgcgagtc ggtgttggaa gaggcctcgg tcgtgttcgt ggaccgtgcc
     2221 gagaaagacg cgggggtcgt gaaggtggag ttggcaacgg ctgtgcccgt tctcgacgcg
     2281 atgcgggaga gcctggtgaa gtccctggag acctctctgg ggaagagagt ggagctgacc
     2341 cttaccgtgg acgagtccgt cctcggcggg gtgaagctgc gcattggaga taggatcgcg
     2401 gacgcgagcc tccggcatcg gctggaaggt ctgcgcgccc gcttggtaag ccccacggca
     2461 aggttggagg gttcagttga agctgcgtcc tgaggagata accgcggtcc tcaagtcccg
     2521 gatcaaggac tatgaggccg aagtcgacat gcaggaggtc ggcaccgtcg tcatgacggg
     2581 tgacggcatc gcccgcatcc atggtctcga gaatgccgtg gcctccgaga tgctcgagct
     2641 tccgcacggg gtgacgggct tggtgctcaa cctcgaggag gacagcgtcg gcgccgtgct
     2701 cctcggcgag agcacgctga tcaaggaggg cgacctggtc aagcgcacag gcaaggtcat
     2761 gcaggtgcct gtgggcgacg ccctcgtggg ccgggtggtg agtcccctcg gcatccccct
     2821 ggacgacaag ggccctattg agaccaccga gttccgtgcg gtggagttca aggcgcccgg
     2881 agtcatccgg cgtcagccgg tgacggagcc cctgcagacc ggtctcaagg ccatcgactc
     2941 catgatcccc atcggtcgcg gccagcgcga gctgatcatc ggcgaccgcg gcacgggcaa
     3001 gacggccatc gcagtggaca ccatcatcaa ccagaagggc caggacgtca tttgcgtcta
     3061 cgtggccatc ggtcagaagg cttccaccgt gcgccaagtg ttggccaagc tcgaggacca
     3121 cggcgccatg gagtacacca tcatcgtgat ggccggggcc tccgaggccg ctccgctgcg
     3181 ctacatcgcc ccgtacgcgg ggtgcgccat ggcggagttc tacacctacc agggcaaagc
     3241 cgccctctgc atctacgacg acctcaccaa gcaggccgac gcctaccggc agatgtcgct
     3301 gctgctccgt cgtcctccgg gccgcgaagc gttcccgggc gacgtgttct acctgcacag
     3361 ccgcctgctg gagcgtgcct gcaagctctc cgacaaggag ggtggggggt cgctcaccgc
     3421 tctgcccatt atcgagaccc aggccggcga cgtgtccgcc tacatcccca ccaacgtgat
     3481 ctcgatcacc gacggccaga tcttcctgga gtcgaacctc ttctacggcg gcgtgcgtcc
     3541 ggccatcaac gtgggcatct cggtgtcccg ggtgggcggc gatgcgcaga tcaaggccat
     3601 gaagaaggtg gccggctcgc tgcgcctcga cctctcccag tatcgcgaac tcgaggcctt
     3661 cgccaagtt
//
LOCUS       DASRMJ010000212         3545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_97197, whole genome shotgun sequence.
ACCESSION   DASRMJ010000212 DASRMJ010000000
VERSION     DASRMJ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97197"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /gene="nuoL"
                     /locus_tag="VFD74_05835"
     CDS             complement(<1..1453)
                     /gene="nuoL"
                     /locus_tag="VFD74_05835"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZK49105.1"
                     /translation="MAFVLVAIFGVPLLVCVVVLAGAAVTSRRGELSARRWARLAGRL
                     SVAGALTALVLAVVGLLSYSAGTDVSVSAVWFHVGDLEVPFSLRLDGLQAVMAVVVAA
                     VAFLVHLFSVGYMRGDRDVSVYFAELSLFTAAMLLLVLSSNLFLLYVAWELVGASSYL
                     LISFYFERPAAAAAGKKAFLTTRVGDLGLLVALFAIVSQTGSFDYDTVFAWAGAPGFD
                     GAWAVAIPLLVFAGAAGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLVARS
                     FPLFAAAPAALAVVAYVGVFTALAAATVAVTQKDIKKVLAYSTISQLGFMMAALGAGA
                     PSAAIFHLFTHASFKALLFLAAGSVIHATRRQAVHELGGLAKSMPVTTWAFAAGGLAL
                     AGIPPFAGFWSKEEVVGALLEHHPVLAVGLLAASLLTAFYIARLFFLVFFGEPRTSGE
                     QEAVRESPWVMTLPLVVLGLAAVFSGFAGARFLG"
     gene            complement(1458..1760)
                     /gene="nuoK"
                     /locus_tag="VFD74_05840"
     CDS             complement(1458..1760)
                     /gene="nuoK"
                     /locus_tag="VFD74_05840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZK49106.1"
                     /translation="MTTAALTLSSILFAVGVFGVLTRRNAISLLVSVEIMMNAANINL
                     VAFSRLHHPEAMTGQTFALLGIGIAACEAAVGLALILAVRRRFGGTDVHIIDLLRG"
     gene            complement(1757..2248)
                     /locus_tag="VFD74_05845"
     CDS             complement(1757..2248)
                     /locus_tag="VFD74_05845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZK49107.1"
                     /translation="MSAQELLFAGFGVVAIAGALYSVVTRSLLRAIIGLGAFLTAVAG
                     EFYLLAADLLAVVQVFVYVGGVVVLMLFALMLTASGARHPLAAIDRPRSGAVLACVIG
                     GGLVWASWTVDFAEAPPAPDDAVGALGRALLGAQLGPFEVMGVILLASVVTALSIVRR
                     ERP"
     gene            complement(2337..3299)
                     /gene="nuoH"
                     /locus_tag="VFD74_05850"
     CDS             complement(2337..3299)
                     /gene="nuoH"
                     /locus_tag="VFD74_05850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZK49108.1"
                     /translation="MSLSGTFAAGLLLGVVSFTLVGVLTAVLVLAERKVGARMQARVG
                     PMHTGPHGSLQTIADMVKLVMKEDVRPARADAVAFGLGPLLVAVPAILSLAVIPFGAP
                     LVMRDLNVGVLYFLAVPGVTVVGLLLAGWASYDNYSFLGGLRSSAQFISYEIPRGLAV
                     VTVVMLAGSLSTVDLLEAQSRVPYIVLLPVSFVIYAVTSLAEVNRVPFDIPEAESELV
                     AGYHTEYSGFRWALFFLAEYAGLFAASAFGALLFLGGGNGPVLPPVVWLLIKTGGLVF
                     VGIWIRFTLPRFRADQLMRLAWKFFLPLSLANLVLAAAVMRAIA"
     gene            complement(3296..>3545)
                     /locus_tag="VFD74_05855"
     CDS             complement(3296..>3545)
                     /locus_tag="VFD74_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZK49109.1"
                     /translation="LKGQAYESGMPQASIPRRPFHMAYYNFALVFIVFEVETLLLFAV
                     APVLRDAGWAGLGEVAAFVALVALSLAYAWRKGALVWK"
BASE COUNT          779 a         1244 c         1126 g          396 t
ORIGIN      
        1 ggcccaagaa cctcgccccg gcgaagccgg aaaacaccgc agccaacccg aggacgacca
       61 gcggcagggt catcacccag ggagactcgc gcactgcctc ctgttctccc gaggtgcggg
      121 gctctccgaa gaacacgagg aagaagagac gtgcgatgta gaaggcggtc agcaggctgg
      181 ccgcaagaag accgaccgcg aggaccggat ggtgctcgag cagagccccc acgacctctt
      241 ccttgctcca gaatccggcg aagggcggga taccggcaag ggccagccct ccggcggcga
      301 aggcccaggt ggtcactggc atcgacttgg ccagcccgcc cagttcatgc accgcctgcc
      361 ttcgggtggc gtggatcacg ctgccggcgg ccaggaacag aagggccttg aacgaagcgt
      421 gtgtgaagag gtggaagatg gcggcgctcg gcgccccggc gccgagcgcc gccatcatga
      481 agcccagctg gctgatggtc gagtacgcca gcaccttctt gatgtccttc tgggtgaccg
      541 cgacggtcgc agcagcgagc gcggtgaaca ccccgacata cgccacgaca gccagggccg
      601 cgggcgcagc cgcgaacagc gggaaagagc gggccacgag atacacgccc gcggcgacca
      661 tggtggcggc atggataagt gccgacaccg gcgtcggccc ctccatggcg tcgggaagcc
      721 atacgtgcag cgggaactgg gccgacttgc ccgccgcacc cgcgaacacc agcagcggta
      781 tcgcgacagc ccacgcgccg tcgaagcccg gcgcgcctgc ccacgcgaac accgtgtcgt
      841 agtcgaaaga acctgtctgc gacacgatgg cgaacaacgc caccagcaat cccaggtcac
      901 ccacgcgggt ggtcaggaaa gccttcttgc ccgccgccgc agctgccgga cgctcgaaat
      961 agaaggagat cagcaggtac gaactcgcgc ccacgagttc ccacgccacg tacagcaaga
     1021 agaggttcga cgacagcacc aggagcagca tggcggcggt gaacagcgac aactccgcga
     1081 agtaaaccga gacatcccgg tcgccgcgca tgtaccccac cgagaagaga tgcacgagaa
     1141 aggcgaccgc ggccacgacc accgccatga ccgcctgcaa cccgtcgagc cgcagactga
     1201 acggcacctc cagatcgccc acatggaacc agaccgcgct cacggacacg tcggtgccgg
     1261 cactgtaaga gagcaggcca accaccgcaa ggacgagcgc cgtgagagcg cccgccaccg
     1321 acaaccgacc ggcaagtcgg gcccatcttc gggcgctgag ttccccacgg cgcgacgtca
     1381 ccgccgcccc cgccagcaca acgacgcaca cgagcagagg gactccgaaa atggccacca
     1441 gcacgaacgc catgccgtca gcccctcaac aggtcgatga tatgcacgtc ggtgccgccg
     1501 aaacggcgcc gcaccgccaa gatcagggca aggcccaccg cggcttcgca cgcggcgatg
     1561 cctatgccca aaagtgcgaa cgtctgcccg gtcatcgcct cggggtggtg aaggcgcgag
     1621 aaggccacca ggttgatatt cgcggcgttc atcatgatct ccacggacac cagcagggag
     1681 atggcattcc tgcgtgtgag cacgccgaac acaccgaccg cgaagaggat ggaggagagg
     1741 gtgagcgccg cggtcgtcac ggtcgctccc gtctcacgat cgagagcgcc gtcaccaccg
     1801 acgccaggag gatcacaccc atgacctcga aggggccgag ctgcgctccc agcagggccc
     1861 gaccgagggc gccgaccgcg tcgtccggcg ccggtggcgc ctcagcaaag tcgacggtcc
     1921 acgaggccca cacgaggccg cctccgataa cacaggccaa gacggccccc gaccgggggc
     1981 ggtctatggc cgcgaggggg tggcgcgcgc ccgacgccgt cagcatgagc gcgaacaaca
     2041 tcagcacgac gacgcccccc acatacacga agacctgcac caccgcgagt aaatcggccg
     2101 caagcagata gaactccccg gccaccgctg tgaggaaggc gcccaagccg atgatcgccc
     2161 tcagaagaga gcgggtgacc accgagtaga gcgctccggc gatcgcgacc acaccgaagc
     2221 cggcgaagag cagctcctgc gcactcacac ctcgcgcttc tccccgcgag cgacgcggcg
     2281 ggcgacccgg ggagcgaccg atggcgcccg gtggccccgc gacgaccgcc gacgtatcac
     2341 gctatcgccc gcatgactgc ggcggccagc accaagttgg ccaaggacag aggaagaaag
     2401 aacttccagg ccaagcgcat gagttgatcg gcccggaacc tgggcagggt gaatcggatc
     2461 cagatcccca cgaacacgag cccacccgtc ttgatcagca gccagacgac cggggggagc
     2521 accgggccgt tcccgccgcc gaggaagagc agggccccga aggccgaagc cgcgaacaga
     2581 cccgcgtact ccgccaggaa gaacagagcc cagcggaatc ccgaatactc ggtgtgatac
     2641 cccgccacca actccgactc ggcctcaggg atgtcgaaag ggacgcggtt cacctccgcc
     2701 aacgaggtca cggcgtagat gacgaagctc acgggcagca gcacgatgta cggaacgcgg
     2761 ctctgagcct ccagaaggtc cacggtgctc agggacccgg ccagcattac cacggtgacg
     2821 accgccagcc ctcgcgggat ctcgtaactg atgaattgag ccgacgagcg cagtccgccg
     2881 aggaacgagt agttatcgta gctggcccat cccgcgagca agagaccgac caccgttacc
     2941 cccggcactg cgaggaagta gagcacgccc acgttgaggt cgcgcatcac caggggcgcg
     3001 ccgaacggga tgacggccaa ggagaggatc gcgggcaccg ccactagtaa cggtcccagc
     3061 ccgaaggcca ccgcatcggc gcgagcgggg cgcacgtcct ccttcatgac gagcttcacc
     3121 atgtcggcga tcgtctgcag cgatccgtgg ggccctgtgt gcatcggccc cacgcgcgcc
     3181 tgcatgcggg cgcccacctt gcgctccgca agcaccaaca ccgcggtgag gacgccgacg
     3241 agagtaaacg aaaccactcc cagcagcagt ccggccgcga aggttcccga gaggctcact
     3301 tccagaccag agcccctttg cgccacgcgt acgcgaggct gagagccacc agtgcgacga
     3361 aagcggccac ctctccgagt cccgcccacc cggcgtcgcg cagaacggga gccaccgcga
     3421 acagcaatag ggtctccacc tcgaagacga tgaagacgag cgcgaagttg tagtaggcca
     3481 tgtggaaagg acgacgcggg atgcttgcct gtggcatccc cgactcgtac gcctgcccct
     3541 tcaac
//
LOCUS       DASRMJ010000213         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_237089, whole genome shotgun sequence.
ACCESSION   DASRMJ010000213 DASRMJ010000000
VERSION     DASRMJ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237089"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFD74_05860"
     CDS             complement(<1..277)
                     /locus_tag="VFD74_05860"
                     /inference="COORDINATES: protein motif:HMM:NF015050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49110.1"
                     /translation="MPADFRIPGCIRGDITGLATLVSCNTPKVPFERSNVVLAKELLR
                     RGVLITTTGCAAHALLDAGLTADAARELCAPGLRAALARADCLPSSPW"
     gene            756..1157
                     /locus_tag="VFD74_05865"
     CDS             756..1157
                     /locus_tag="VFD74_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49111.1"
                     /translation="MTRRISVPRVFHVFALFLGALSLLGALGSALWVLVSFGPPMADD
                     NYGPAFWAFVLVVLGVITAFSAWKRRPEPVWLAAFALLVIGIGGIWTIGFVVIAVACP
                     VALTAFVLSCNRLLSWWLRLPIEQLHPGEGR"
     gene            1333..1857
                     /locus_tag="VFD74_05870"
     CDS             1333..1857
                     /locus_tag="VFD74_05870"
                     /inference="COORDINATES: protein motif:HMM:NF020289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49112.1"
                     /translation="MAGRRRWEIRRSRHRGCPLSLPPGRFPPRPHRATARSEVCRAKA
                     RTGSDWTPCWGEAKCLPSLAADGEHWDQDPWLLGVGNGVLDLRTGILRPGRPEDNVTC
                     FCDVVYDPAAVCPRWLTFLTEVFDGDADLIDFAWRVTGYALSGITIEQVLFLCHGRGA
                     NGKSIFRALLRTRR"
     gene            2170..>2418
                     /locus_tag="VFD74_05875"
     CDS             2170..>2418
                     /locus_tag="VFD74_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49113.1"
                     /translation="MRTLRKMRTVPLLLPLLLTVMLVVPAAAMAAQPPVDLGTTSSFA
                     VLAGSTITNTGPTTINGDAGGDVGLYPGASFTGQSSVTL"
BASE COUNT          398 a          804 c          760 g          456 t
ORIGIN      
        1 cccacgggga tgacgggagg cagtccgccc gcgccagagc ggcgcgcagt ccgggggcgc
       61 acagctcccg cgccgcgtcg gccgtcaatc cggcgtcgag cagggcgtga gcggcgcatc
      121 cggtggtggt gatgagcact ccccgacgca aaagctcctt ggcgagcacc acgttcgaac
      181 gctcgaaggg cactttgggc gtattgcacg acaccagggt ggccagaccg gtgatgtcgc
      241 cccggatgca tccggggatc cgaaagtccg ccggcaccct cgcctgcccc gcggcgagga
      301 gatactgaca cttcttcgga tggctagcat gaaaagagcc tagcatcgct atcggacaga
      361 accgtatgga taatcacgga gggtacgtgg cctcggcccg gaactacccc tctcgcagcc
      421 atgtggttcc ctgtccgtcc gtgcatcggc atatcgcggc cgcttcgggt ttccggttac
      481 gtggtgacgg actcctcttg tttggcactc tcactttagt ccgctacaca aggaagagca
      541 aagctgcctt ccctgtgcct atccggcacg aattcgtgga ttgatggagt acgctgacca
      601 acgccccgcc tttgaggacg gccacctcgc cgatcacgac acaggcccgt gcgccctatc
      661 ccgcggtggt gcttgccttc tgacgtttca gcgtggctag cactggggat ttgataggtg
      721 tcgacgccca gctcgccctg aggagatggc ggcccatgac ccggcgcatc agcgtgccac
      781 gcgtcttcca cgtcttcgcc cttttcctgg gtgcgttgtc gctcctcggt gccctcggca
      841 gcgcgctctg ggttctcgtc agctttgggc cgcccatggc cgacgacaac tacggccccg
      901 ccttctgggc cttcgtgttg gtcgtgctcg gggtcatcac cgccttctcg gcctggaaac
      961 ggcggccgga gccggtgtgg ctcgccgcct tcgccctcct ggtcattggc atcggcggca
     1021 tctggaccat cggttttgtg gtaatcgccg tcgcctgccc ggtcgctctg accgcgttcg
     1081 tcttaagttg caaccgtctg ctgtcgtggt ggttgcgcct cccgattgag cagctgcatc
     1141 ccggcgaggg acggtaggaa cgctcacgga ggctggcagg tactggtcga gagtggggaa
     1201 actactcgcg gcgctcgctt tccttgcctc gctcgccgac gccggcaaca gggagctctt
     1261 cgcgcatctc aaccgcgacc agatgcgcca cgaccaccgt cgccagcact ggcccatctg
     1321 gggtgagcat cattggcggg aaggcgcaga tgggagatca ggcgttctcg ccatcgaggc
     1381 tgcccgttat cgctaccacc tggccgattc cctcccagac ctcaccgagc aacagctcga
     1441 agcgaagttt gccgtgcaaa agcgagaacc ggcagcgatt ggacgccatg ctggggggaa
     1501 gccaagtgcc tcccatcctt ggcggccgac ggagagcact gggatcagga cccttggctc
     1561 ctcggcgtgg ggaacggggt gcttgatctg cgcaccggca tcctgcggcc cggccgccca
     1621 gaagacaacg tcacctgctt ctgcgacgtc gtctacgatc cggcggccgt gtgcccgcgc
     1681 tggctcacct tcctcacgga ggtgttcgac ggtgacgccg acctcatcga cttcgcctgg
     1741 cgcgtgacgg gctacgcgct gagcggcatc accatcgagc aggtgctctt cctctgccac
     1801 ggccgaggcg cgaacggcaa gagcatcttc cgcgccctcc tgcgcacgcg ccgatgacct
     1861 gttccgcacc gaccgcgcgc cccagtgttt caccaacttc taggggccgc agcctctggg
     1921 cgatccgtcg cccccgcggt tcacacggct gattcatgcg cgtttcgacc ttggccggag
     1981 actcgagtgg gaccgcgtcg cccccgcctc cgcggggccg cagggtctgg acgtttgcag
     2041 gagactccac ctgaagacga gaatcttcat cagatagtct cgtcgcctac tggacaagtc
     2101 gggggaagag catctagaat taagagcatg tgtgcatgaa aagttgacaa tggggggtcg
     2161 aatcgactca tgagaacact caggaagatg cgaaccgtgc cgctgctgct accgctgctg
     2221 ctaacggtca tgctggtggt acccgcggcg gctatggcag cccaaccgcc ggtggacctc
     2281 ggaacgacct ccagttttgc ggtcctggcc ggctcgacga tcaccaatac cggaccgacc
     2341 acgatcaacg gagacgccgg cggagacgtc ggcctctatc ctggtgcgtc tttcaccgga
     2401 cagtcgagcg tgacgctg
//
LOCUS       DASRMJ010000214         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_419233, whole genome shotgun sequence.
ACCESSION   DASRMJ010000214 DASRMJ010000000
VERSION     DASRMJ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419233"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFD74_05880"
     CDS             <1..940
                     /locus_tag="VFD74_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZK49114.1"
                     /translation="RNRIIMPPMANNKADAQGYVTDTLVDHYARHAAGGPGMVVVEHS
                     YVTLTGRVDTHQLGIWSDDHVAGLGRIADAIHEGHAAAVIQITHGGARCPREAIIATA
                     VSPSGIAVPGDTEEPRLMELDEIEALPELFSAAARRAREAGFDGVEVHGAHGYLLDQF
                     MSPYTNHRDDAYGGSLEHRLRLIRETLEAVRRELDHGLLMYRLGVDDAPVPGITPAGA
                     AEIAVYLEDWGVDLIDCSGGLCGSRPADRTEPGYFVEAGAIVKRAVSIPVAAVGGITD
                     PAYADGLIRRGDLDAVCVGRAQLKDAEWARKALAGL"
     gene            1003..>2222
                     /locus_tag="VFD74_05885"
     CDS             1003..>2222
                     /locus_tag="VFD74_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HZK49115.1"
                     /translation="MPTNILTEKHRKLLAEERAVLADLQVILARHDAGREDQQVLEHS
                     IRQLDELFLLVVAGEFNSGKSTFINALLGTRLLEEGVTPTTSRIHTLVYGNDATSSVD
                     GEGIQRVQAPVDLLREVEIVDTPGTNALEREHEAITQRYIPRSDLVLFVTSADRAFTE
                     SERAFIEQIRGWGKKLVFVINKADILRSADEVAEIERFVVENAQRLLGFSPEVFSVSS
                     RLALEAKLAGSPEGRLMEESRFDAFETYLVDTLDEGERVRLKLLNPLGVGLNLAHKYS
                     EAVQGRLHILADDLSSLDSIDHQLDIYREDLAREFRFRLSDVDNVLHEFEQRGQQFLD
                     DTIRLGRIFDLVKKTRVREEFERMVVTDAPAQVERKVSEIIDWMVAAELRQWQTVNQH
                     VLERRVHHSESVIG"
BASE COUNT          415 a          744 c          725 g          338 t
ORIGIN      
        1 gcgcaaccgc atcatcatgc cgccgatggc caacaacaag gccgacgccc agggctacgt
       61 caccgacacg ctcgtcgatc actacgcgcg acatgccgcc gggggtccgg gcatggtcgt
      121 ggtggagcac tcctacgtca ccctcaccgg ccgcgtggac acccaccagc tcggcatctg
      181 gagtgacgac cacgtcgccg gcctgggccg catcgccgac gccatccacg agggccacgc
      241 ggcggcagtg atccagatca cccacggggg tgcccgctgc ccgcgagagg ccataatcgc
      301 cacggcggtg agcccctcgg gcatcgccgt tccgggcgac acagaggagc cccgactgat
      361 ggagctcgac gagatcgagg cgctccccga gctgttctcc gcggccgccc gccgcgctcg
      421 ggaggcgggc ttcgacgggg tcgaggtcca cggcgcccac ggctacctgc tcgaccagtt
      481 catgtccccc tacacaaacc accgcgacga cgcctacggc ggaagtctgg agcaccgtct
      541 tcgcctgatc agagagactc tcgaggctgt caggcgcgag ctcgatcacg ggctcctcat
      601 gtaccgcctg ggcgtggacg acgcccctgt ccccggcatc accccggcgg gcgcggccga
      661 gatcgccgtg tatctggaag actggggcgt tgacctcatc gactgcagcg gcggactctg
      721 cggctcgagg ccggcggatc gcaccgagcc cggctacttc gtggaggcgg gtgcgatcgt
      781 gaagcgggcc gtgtcgatcc cggtagcggc cgtgggcggc atcaccgatc ccgcctacgc
      841 cgacgggctc atccgccgag gcgacctgga cgccgtctgc gtggggcggg cacaactcaa
      901 ggatgcggag tgggcccgga aggcgcttgc gggcctgtaa ggtggacgcg gcacaaaggg
      961 tttcgaagcc cggcaccgga gggtaagcgc cgccaggaaa ccatgcctac gaacatcctc
     1021 acagagaaac acagaaagct gctcgcggag gagcgggccg tgctggccga cctgcaagtc
     1081 atcctcgccc ggcacgacgc cggcagggaa gaccagcagg tcctggagca ctcgatccgc
     1141 caactggatg agctcttcct gctcgtggtg gccggggagt tcaactcagg caagagcacc
     1201 ttcatcaacg ccctcctcgg cacgcgactg ctcgaggagg gcgtgacacc cacgacctcg
     1261 cgcatccaca cgttggtcta cggcaacgac gcgaccagta gtgtggacgg cgaaggcatc
     1321 cagcgcgtgc aggctccggt agatctactg cgcgaggtgg agatcgtcga cacgccgggc
     1381 acgaacgccc tggagcgcga acacgaggcc atcacccagc ggtacatccc gcgctcggac
     1441 ctcgttctct tcgtcacctc agccgatcgc gccttcaccg agagtgagcg ggccttcatc
     1501 gagcagatcc gcggctgggg taagaagttg gtgttcgtga tcaacaaagc cgacatcctc
     1561 cgttcggccg acgaggtggc cgagatcgag aggttcgtcg tggagaacgc ccagcgtctc
     1621 ctcggtttct cacccgaggt gttctctgtg tcgtcccgac tggctctgga agccaagttg
     1681 gccggttccc ccgagggcag gctcatggag gagagccgct tcgacgcctt cgagacctat
     1741 ctcgtcgata ccctggacga aggcgaacgc gtgcggctga agttgctcaa tcccctgggc
     1801 gtgggcctga accttgccca caagtacagc gaggcagtcc agggtcgcct gcacatcctt
     1861 gccgatgacc tctcctccct cgacagcatc gaccaccagc tcgacatcta ccgggaggac
     1921 ctcgcccggg agttccgctt ccgcctgagc gacgtggaca acgtgctgca cgagttcgag
     1981 cagcgcggcc agcagttcct ggacgacacc atccgcctgg gacgaatctt cgatctggtg
     2041 aagaagacac gcgtgagaga ggaattcgag cgcatggtgg tcaccgacgc ccctgcgcag
     2101 gtagaacgca aggtgagcga gatcatcgat tggatggtgg ccgctgaact caggcagtgg
     2161 cagacggtga accagcacgt cctggagaga agagtccatc actccgagag cgtgatcgga
     2221 ag
//
LOCUS       DASRMJ010000215         5660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_419311, whole genome shotgun sequence.
ACCESSION   DASRMJ010000215 DASRMJ010000000
VERSION     DASRMJ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419311"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..435
                     /locus_tag="VFD74_05890"
     CDS             127..435
                     /locus_tag="VFD74_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZK49116.1"
                     /translation="MVAVLESKFDAIEALCRRYGVVRLFVFGSALRDDFREGESDVDL
                     LVEFGPMDPFALVDAYFGLLNAFRGLLGTKVDLVMSDAIKNRYIAAEVDRTKQILYAA
                     "
     gene            425..784
                     /locus_tag="VFD74_05895"
     CDS             425..784
                     /locus_tag="VFD74_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HZK49117.1"
                     /translation="MQREVQAYVLDIVEACDAIAIAVHGVDVDAYRTNRLIRSAVERE
                     FITIGEAVAVLRRLAPDMFARITQAQQIVDFRNQLTHGYMTVNDTIVWGVAVRDSPLL
                     RGECADLLSELDESFAD"
     gene            890..1240
                     /locus_tag="VFD74_05900"
     CDS             890..1240
                     /locus_tag="VFD74_05900"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZK49118.1"
                     /translation="MAQLTTDIGATMAQDPQLAELVKRLVAAYSPEQVFLFGSRARGE
                     AGPDSDYDLLVVVPDDSAVDRTTSRLGYKALRGTGVAADVVVWRRSSFERRAHVRASL
                     PYAALHEGVVLHGG"
     gene            1230..1670
                     /locus_tag="VFD74_05905"
     CDS             1230..1670
                     /locus_tag="VFD74_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HZK49119.1"
                     /translation="MAVDPARAEETIAWLTKADEDLRAARVLIDIREPLLETAVFHCQ
                     QAAEKAFKAFLVWHDVPFRRTHSLEEIGEQCLQVDADLTELVDEAAPLGEYAWRYRYP
                     GEAEYLGLDEVRDALDVSSRVLLAVRERLDSDDDFVKSRHIDAE"
     gene            1694..1903
                     /locus_tag="VFD74_05910"
     CDS             1694..1903
                     /locus_tag="VFD74_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017714909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZK49120.1"
                     /translation="MTKYEIIIYWSEEDGAFVAEVPALPGCMADGATYEEALASVNLV
                     AQEWIETGVELGRSVPEPKGRLMFA"
     gene            1968..2690
                     /locus_tag="VFD74_05915"
     CDS             1968..2690
                     /locus_tag="VFD74_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HZK49121.1"
                     /translation="MSTTTIEWTETTWNPVTGCSKVSPGCFNCYAERMAKRLQAMGQP
                     NYRDGFAVRVHDHMLGVPTRWAKPRMVFVNSMGDLFHENVSTHFIRRVFQVMERTPRH
                     TYQLLTKRADRLAEVAPALVWPRNVWMGVTVEDNEHLSRVERLREVPSAVRFLSIEPL
                     LGRLPALNLDGIDWVIVGGESGPGARPMHENWVLEVRDACLDRAVPFFFKQWGGTRKK
                     AAGRELESRTWSEMPCRPVGVA"
     gene            2782..3087
                     /locus_tag="VFD74_05920"
     CDS             2782..3087
                     /locus_tag="VFD74_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZK49122.1"
                     /translation="MSASSPKELVWIASSLKELREFPEDVRQVMGFALFTAQSGGKHV
                     AAKPLQGYRGAGVLEVVDDYDGDTYRTVYTVRFAEAVYVLARQIHAQRLSSRKGDTR"
     gene            3084..3452
                     /locus_tag="VFD74_05925"
     CDS             3084..3452
                     /locus_tag="VFD74_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK49123.1"
                     /translation="MTGHDIDHEFSSGNVFADLELTASDELLAKAELVRQITSIATHR
                     HLTQAETASILGTTQPKVSDLFTGRLAGFSMERLIRFLNALDRDVRIVVLPKPRSRER
                     ATTSVTASRAALPRLRVSRV"
     gene            3521..3868
                     /locus_tag="VFD74_05930"
     CDS             3521..3868
                     /locus_tag="VFD74_05930"
                     /inference="COORDINATES: protein motif:HMM:NF022671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HZK49124.1"
                     /translation="MAFSEVELKRIDATIGALCRRRSPAEYADQLRFAYEIDGHSVSI
                     LEQRPPWDGSPSGWTSHGVARFRYFRSRDEWQLYWMRRDMKWHPYEPVTSTKGLARLV
                     KLVEDDEHCAFFG"
     gene            4026..4982
                     /locus_tag="VFD74_05935"
     CDS             4026..4982
                     /locus_tag="VFD74_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK49125.1"
                     /translation="MEHANPMLLEPVETVEILVLMDNFSDVLLSSTEHAVRPPLAPGG
                     NIPATTLLAEHGLSLLITVRGKSATSRLLLDAGYSDVGVPHNLDLLGVDLSDLEALVL
                     SHGHMDHFGALGSVLQRVGEQVPVVAHPAALEGCRYLDRPGAGLARFVDLPPEVVASL
                     GSRLRLSAGPYVSPGRLWATTGEVARETAFEKGMPGALRERDGRMVADSLEDDTAVVV
                     NVGGKGLVVISGCAHAGIVNTVRQARDITGVEKVYALVGGFHLGGATLAEVVDLTVEA
                     IREFAPQVVVPMHCTGRAATQRLEGVFGDAFEVSSVGTMFRL"
     gene            5020..>5660
                     /locus_tag="VFD74_05940"
     CDS             5020..>5660
                     /locus_tag="VFD74_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZK49126.1"
                     /translation="MTLQVEAVRAEFPALREGAAHFDAPGGSLTPAVVAEAIRDTLGA
                     AVCQRGSITIAERRTDEIVLGARQAVADLLGADPGGVVFGRSMTQLTFDFSRTLAKSW
                     REGDEIVVSRLDHDANIRPWVYAAESVGVRVRWVEFDPATSEITVEDVEEQLSDHTRL
                     VAVTAASNLIGTRPPIKEIAQRVHERGALLYVDGVHYTPHALVDVVDMGADFYA"
BASE COUNT         1015 a         1687 c         1921 g         1037 t
ORIGIN      
        1 gcactgctat gcaggcgagg ggagcatgtc gctatcaggg catgcattag acgggttcgt
       61 tggccacagg ctcgctcaca tggccggcgg gtcccgcagg gggtaggata gcgtcggagg
      121 ttcgacatgg ttgctgtgct cgagagcaag tttgacgcca tcgaggcttt gtgccgccgg
      181 tacggggtcg tgcgtctgtt cgtgttcggc tccgcattgc gcgacgactt ccgggaggga
      241 gaaagcgacg tcgacctgct cgtggagttc ggcccaatgg acccattcgc gctcgtcgac
      301 gcgtacttcg ggctactcaa tgcgttccgc gggctcttgg gcacgaaggt cgacctggtc
      361 atgagcgatg cgatcaagaa tcgctacatc gccgctgagg tcgatcgcac gaagcagatt
      421 ctctatgcag cgtgaggtgc aagcgtacgt cctcgacatc gtcgaggcct gcgacgcgat
      481 cgcgatcgca gtccacggcg tggacgtgga tgcgtaccgg accaatcgcc tcatccggtc
      541 ggcggtcgag cgagagttca tcaccatcgg cgaggccgtt gcggtactgc ggcggctcgc
      601 gcccgatatg ttcgcgcgca tcactcaggc gcagcagatc gtggacttcc gtaaccagct
      661 gacgcacgga tacatgacgg tgaacgacac gattgtgtgg ggagtcgcgg tccgtgactc
      721 gccactgctc cggggcgagt gcgccgatct gctgtcggag ctggacgaat cgtttgcgga
      781 ttgacaagcg ggtcgactcg accgcaccgc caccctgcag tcgcagttcg cgcaggctgt
      841 tcgccgcctc tccggccggg gtgctacgat aggtgccagg agacaccaga tggcacagct
      901 gacaaccgac attggagcca ccatggcaca agatccgcag cttgcggaac ttgtgaagcg
      961 cctggtcgcg gcctacagcc cggaacaggt gttcttgttc gggtcgcggg ctcgtggcga
     1021 agctggacct gatagcgact acgatctgtt ggtggtggtc ccggatgaca gcgccgtgga
     1081 ccgtaccaca agccgtctgg ggtacaaggc gcttcgcgga acaggcgtcg ccgccgacgt
     1141 ggtggtttgg cgccgcagct ccttcgagcg tcgagcccac gtgcgggcct cgcttccgta
     1201 cgctgcgctc catgagggtg tcgtgcttca tggcggttga cccagcccga gccgaggaaa
     1261 ccatcgcgtg gcttaccaaa gccgatgagg acttgcgggc cgcaagagtc ctgatagaca
     1321 tccgggagcc gctcctcgag acggccgtct ttcactgcca gcaggcggcc gaaaaggcct
     1381 tcaaggcgtt tctcgtctgg cacgatgtcc cattccgtcg gacgcacagc cttgaggaaa
     1441 ttggtgagca gtgcctgcag gtcgatgccg atctgaccga actcgtggat gaggctgcgc
     1501 ccctcggcga gtacgcgtgg cgctaccgct atccaggtga ggcggagtat ctcgggctcg
     1561 acgaggtcag ggacgcgctt gacgtgagca gccgagtctt attggccgtg agagagcgcc
     1621 tggattccga cgacgatttc gtgaaatcga ggcacatcga cgccgagtag catcgattgg
     1681 gggacacgga gtcatgacaa agtacgagat cattatctac tggagcgaag aggacggcgc
     1741 cttcgtagcc gaggtgccgg cactcccggg ttgcatggcg gacggagcta catacgagga
     1801 agccctggcc agtgtgaatc tggtggcgca ggaatggata gagaccggtg tggaactcgg
     1861 ccgatccgtt ccggagccga agggcaggtt gatgttcgcc taacttgtgg cccggcgcga
     1921 tcgaggccat tgggaagtca tggttcacat tggggcttga ccgaatcatg agcaccacga
     1981 ccatagagtg gacggagaca acgtggaatc ccgtcactgg gtgctcgaag gtgtccccag
     2041 gctgtttcaa ctgctacgcg gaacgcatgg ccaagcggct gcaggctatg ggacaaccaa
     2101 actaccgcga tggctttgcg gttcgcgtcc acgatcacat gctcggagtg cccacgaggt
     2161 gggcgaagcc acgtatggtc ttcgtcaact cgatgggaga cctgtttcac gagaacgttt
     2221 ccactcactt catccggcgg gtattccagg tcatggagcg aacgcctcgg cacacatacc
     2281 agctgcttac caagcgcgca gatcgcctcg cagaggttgc tccggccctt gtgtggcctc
     2341 gaaacgtctg gatgggagtc actgtagagg acaacgagca cctcagccgt gtggagcgac
     2401 ttcgtgaagt cccctcggcc gtcaggttcc tgtccatcga gccccttctc gggcggctgc
     2461 ccgccttgaa cctcgacggc atcgactggg tgatcgtcgg cggtgaatcc ggacccgggg
     2521 cgcggcccat gcacgagaac tgggtgctgg aagttcgcga tgcctgcctt gacagagcgg
     2581 tgcccttctt cttcaagcag tggggcggaa cacggaagaa ggcggccggc agggagttgg
     2641 agagccgcac atggagtgaa atgccgtgca ggccggtcgg tgtggcctga tgaccagaag
     2701 cacccggagg cgacgtcgag ggcttcctga gggacggggt aatttgtccc cagaatatcc
     2761 cagttctggt atacttccca cgtgagcgca tcgtcgccaa aggaactggt gtggatcgcc
     2821 tcgtctctga aagagctacg agagttcccc gaggacgttc gccaggtgat gggtttcgcg
     2881 ctcttcacgg ctcaatccgg cggtaagcac gtcgcggcga agcctcttca ggggtatcgt
     2941 ggcgccggag tgctcgaagt ggtggacgac tacgatggcg acacctatcg gacggtgtac
     3001 acggtgcgtt tcgcagaagc cgtctatgtg ctcgctcgac agatacacgc gcaacgcctg
     3061 tcttcgcgga agggagatac aagatgaccg gccacgacat cgaccacgag ttcagtagcg
     3121 gcaacgtgtt cgcggatctt gagctcaccg cttcagatga actgctggcc aaggcggaac
     3181 tcgtgcgcca gatcaccagc atcgccaccc accggcacct tactcaggcg gagacggcaa
     3241 gcatccttgg aacgacccag cccaaggttt cggacctctt cacgggcagg ctcgccggtt
     3301 tctcgatgga gcggctcatc cggttcctga acgctctgga tcgtgacgtt cgcatcgtgg
     3361 tgctccctaa gccgcgcagt cgtgagcgtg ccaccaccag cgttactgct tcgcgcgcgg
     3421 cgctaccccg gctgcgggta tcccgcgtct gaacgagatg atcgcccacg ggaccgatgg
     3481 tcgcagcagc gtccactcgg gcgaggaatt ggggccacag gtggcattca gcgaagtcga
     3541 gttgaagcgg atcgacgcca cgatcggcgc gctctgccgc agaaggtcgc ctgcggagta
     3601 tgccgatcaa ctgcggttcg cgtacgagat cgacggccac tccgtttcca ttctggagca
     3661 gcgcccgccg tgggacggca gccccagcgg atggacgagc cacggagtcg caaggttcag
     3721 gtacttccgt tcgcgagacg aatggcagct gtactggatg cgcagggata tgaagtggca
     3781 cccctacgag ccggtgacgt ctaccaaggg cctggccagg cttgtgaagc tggttgagga
     3841 cgacgagcac tgtgccttct tcggatagtg tgatcctcgc gccggcggcg caagttgccg
     3901 gtggtcatca acgcggctgg tgcggaagtc tagttgatgc cgcgctcggc tgctgatgtg
     3961 ctggccgtgt cggcgcgtcc ccgatagact gggtcagcac tccgtgccgc agagagggag
     4021 gctccatgga gcacgcgaac cccatgttgc ttgagccggt cgagacggtg gagatcctcg
     4081 tcctcatgga caacttcagc gacgttctgt tgtcctccac ggagcacgcc gtccgtccgc
     4141 cgttggctcc cggcgggaac atccctgcca cgaccctgct ggccgagcac gggttgagcc
     4201 tgctgatcac cgtgcgcggc aagtccgcga cgtcgcgcct tctcctggat gccgggtact
     4261 cggacgtagg ggtcccccac aacctggacc tcctgggtgt agacctctcc gatctggagg
     4321 cgctggtgct gagccacggc catatggacc acttcggggc cctgggctcg gtgctccagc
     4381 gcgtggggga gcaggtgccg gtggtggcgc accctgccgc gcttgaaggg tgccgctacc
     4441 tcgaccgacc cggggctggg ctcgcccggt ttgtggacct gcctccggaa gtggttgcgt
     4501 ctctcgggag ccggttgcgc ctgtcggccg gcccctacgt gagccccgga agactctggg
     4561 cgacgaccgg ggaggtggcc cgggaaacgg cctttgagaa ggggatgccc ggggcgttgc
     4621 gggaacggga cggccggatg gtggccgact cactggagga cgacacagcg gtcgtggtga
     4681 acgtaggcgg gaaggggctg gtggtcatct ccggatgcgc gcacgcgggg atcgtgaaca
     4741 cggtccggca ggcccgggat atcaccggcg tcgagaaggt atacgcgctc gtgggagggt
     4801 tccatctggg aggcgcgacg cttgccgagg tcgtggacct cacggtggag gcgatccggg
     4861 agttcgctcc gcaggtagtc gtgcccatgc actgcacggg ccgcgcagcc acgcagcggc
     4921 tggagggcgt cttcggggac gccttcgagg tgagcagcgt gggaaccatg ttccggctct
     4981 agggaccttg accaccatcc gaacaaaggg cgaaacgaga tgacactgca ggtcgaagct
     5041 gtccgagctg aattcccggc actgcgcgaa ggtgccgcac acttcgatgc gccgggtggt
     5101 tccctcaccc cggctgtcgt cgccgaagcc atcagggaca cccttggagc ggccgtgtgc
     5161 cagcgaggat cgatcaccat cgcggagcgt cgcaccgatg agatcgtcct cggcgcgcgg
     5221 caggcggtgg cggacctcct cggagcggat cccggaggcg ttgtattcgg gcgaagcatg
     5281 acgcaactca ccttcgactt ctcccgcact ctggcgaagt cgtggaggga gggcgatgag
     5341 atcgtcgtct cgagactcga tcacgacgcg aacatccgcc cgtgggtata cgccgcggag
     5401 agcgtcggcg tgcgggtgcg ctgggtggag ttcgatccgg cgacttccga gatcaccgtc
     5461 gaggacgtcg aagagcagct ctccgatcac accagactgg tagccgtcac tgctgcctcg
     5521 aacctcatcg gaacgcgccc gcctatcaag gagatcgccc aaagggtcca cgagcgcggc
     5581 gcccttctct atgtcgacgg agtgcactac acgccccatg cgctcgtcga tgtggtggac
     5641 atgggtgcag acttctacgc
//
LOCUS       DASRMJ010000216         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_419320, whole genome shotgun sequence.
ACCESSION   DASRMJ010000216 DASRMJ010000000
VERSION     DASRMJ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419320"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..512
                     /locus_tag="VFD74_05945"
     CDS             123..512
                     /locus_tag="VFD74_05945"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HZK49127.1"
                     /translation="MSDQTRPAWIESAPVVLEIEAREMHERGEDPFFAVMDGARGVGL
                     GEAFRVRNTFPPVPLFGVLEKKGFSHWAEELGPQDWLITFYRERAVAPDRDADDTHPK
                     GAVRPESMPSPTPPTAAAAAAEADEQG"
     gene            complement(559..939)
                     /locus_tag="VFD74_05950"
     CDS             complement(559..939)
                     /locus_tag="VFD74_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZK49128.1"
                     /translation="MFKRIDHVEIVPSDYERAISFYCDVFGFAIRQRVPLGLAHIREV
                     VFLELGDTLLEVLDIVDPEPVGVRVERVGYRMMALEVDSMDDALAYLSGKGIEPSRAP
                     YPSAGGFRAEVLDPDGLRIEIRQW"
     gene            1106..>2110
                     /locus_tag="VFD74_05955"
     CDS             1106..>2110
                     /locus_tag="VFD74_05955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK49129.1"
                     /translation="MKDGLTDDLIDDRPIDYRTISMWHDTAGDDFSPRPSLSGPTDVD
                     VAIVGAGYTGLWTAYYLKKADPSLRVAVLEREVAGFGASGRHGGWCSARFATPREKMA
                     AVGGRQGVIDMQRAMFDTVYEVGRVAEVEGIDCDFHVGGNLVFATNNAQIGTIRDKVL
                     AERSWGFGDEDFRWLAPDEARRHVTVEGVRGAIYCPHSARVHPTKLARGLARVVEGMG
                     VPIYERTPALELGHGGVLTPAGRVKADVVVRATESYSVELPGLRRSSVPVYSLMIATE
                     PLADDVWDQLGWSGHATWSDGRHLIIYVARTADGRIAFGGRGTPYHRDSRISPAYEQD
                     P"
BASE COUNT          365 a          721 c          706 g          318 t
ORIGIN      
        1 tgcctcgaca gggcataggt gggcggactg cacggtgcac cctgacacct gtgacgaccc
       61 ggctgggtag gccgacgagt cgggtgtact tcgatccatc atctacctgg aggtaggcga
      121 ccgtgagtga tcagaccaga ccggcttgga tcgagtccgc gcccgtggta ctcgagatcg
      181 aggcgcgcga gatgcacgag cgaggcgagg atccgttctt cgccgtcatg gacggggcgc
      241 gcggtgtagg gctgggcgaa gccttccgcg tgcgcaacac cttccctccc gtccccctct
      301 tcggggttct tgagaagaag gggttctcgc attgggccga ggaactcggc ccgcaggact
      361 ggctcatcac cttctaccgg gaacgcgcgg tggcccccga ccgagacgcc gacgacacgc
      421 atcccaaggg agcggtccgc ccggagagca tgcccagtcc cacgccgccg accgccgccg
      481 cggcggccgc ggaagcggac gagcaaggct agcttcagcc ggaccggcgg actcgcccgg
      541 cgggtctagg ccgaccggct accactgccg tatctcgata cgcaacccgt cgggatcgag
      601 cacctcggca cggaagcctc ccgccgacgg gtagggcgcg cgggaaggct cgatgccctt
      661 gccggagagg taggcgaggg catcgtccat actgtccact tcgagcgcca tcatccggta
      721 gcccacccgc tccacgcgga cacccaccgg ctcgggatcc acgatgtcga gcacctccag
      781 tagagtgtca ccgagttcaa ggaacacgac ctctcggatg tgcgcgagcc ccagcggcac
      841 ccgctgacgg atcgcgaacc cgaagacgtc gcagtagaag gagatcgccc gttcgtagtc
      901 tgagggcacg atctctacgt ggtctatccg tttgaacact tgcgtctcct tcgatcgggg
      961 ccgctaccag acgcggcaca ctacgcgcag tctacctgtt cgcggcgatg gtaggattgg
     1021 ggtgaagatc ctggccaccg ccaccaggcc cacgctacgc ggagatgcac cgggcgctgc
     1081 agtagagacc catgtggagg acccgatgaa ggacggctta accgacgacc tgatcgacga
     1141 tcgcccgatc gactacagaa cgatcagcat gtggcacgac acggccggag acgacttctc
     1201 tccacggccc tccctcagcg gccccacgga cgtcgacgtg gccatcgtcg gcgccggcta
     1261 tacgggactc tggacggcgt actacctcaa gaaagccgac ccctctctgc gggtggcggt
     1321 gctggagcgt gaggtcgcgg gcttcggagc ctccgggcgt cacggaggct ggtgctccgc
     1381 ccggttcgca acgccgcgcg agaagatggc ggcggtcggc ggtcggcagg gcgtcatcga
     1441 catgcaacgg gcgatgttcg acacggtgta cgaagtcggc cgcgtggccg aggtcgaggg
     1501 tatcgactgc gacttccacg tcggcggcaa cctagtattc gccacgaaca acgcccagat
     1561 cggcaccatc agggacaagg tcctggccga gcgctcctgg ggcttcggcg acgaggactt
     1621 ccgctggctg gcacccgacg aagcccgccg ccacgtcacc gtcgagggtg tccgcggcgc
     1681 gatctactgc cctcactcgg cccgcgtgca cccgacgaag ctggcccgcg gtctcgcccg
     1741 cgtcgtggag ggtatgggcg tgcccatcta cgagcgcacg cctgcactcg aactcggcca
     1801 cggcggcgtg ctcacgcccg ccggccgagt caaggccgac gtcgtggtga gagccaccga
     1861 gagctactcg gtggagttgc ccgggctgcg gcgctcgtcg gtgccggtgt actcgctgat
     1921 gatcgccacc gagcctctcg ccgacgacgt gtgggaccag ttgggctgga gcggacacgc
     1981 cacctggagc gacgggcggc atctcatcat ctacgtggcc cgcaccgccg acggacggat
     2041 cgccttcggt ggacgcggga cgccctacca ccgagactcc cgcatctcgc ccgcctacga
     2101 gcaagatccg
//
LOCUS       DASRMJ010000217         3816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_160923, whole genome shotgun sequence.
ACCESSION   DASRMJ010000217 DASRMJ010000000
VERSION     DASRMJ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160923"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFD74_05960"
     CDS             complement(<1..711)
                     /locus_tag="VFD74_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZK49130.1"
                     /translation="MRGPRAGSDWPAIIQGGMGVGVSGWGLARAVAIAGQLGVVSGVA
                     LDTLIARRLQLGDPGGHTRRALTSFPYADVSEQIVERYFVAGGIGPEEPFRSVPRLSL
                     HPSRRATDLVIAANFVEVFLAKEGHDGLVGVNYLEKLQMATPAATYGAMLAGVDVVVV
                     GAGIPDELPGLLDALAAGRAGEVAVDVHGAGGTRFAVAVRPEPISGANVELRRPKFLA
                     IVASHVLASYLARDIRTRP"
     gene            994..1605
                     /locus_tag="VFD74_05965"
     CDS             994..1605
                     /locus_tag="VFD74_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZK49131.1"
                     /translation="MSPARLLLLDEPMAALDLGTRGSVRHHLRRHLAEITGSCVLVTH
                     DPLDAAAIADRLVIIEAGRLAQQGTLKEITTRPRTAYIAGLMGLNLLSARARGTTLTL
                     ANGATLEAASPTTGEVFVVIAPQSIALYPGRPEGSPRNTWPTRVSEIHLMGDRVRVLV
                     DEPVPVTAEITAVSLAELGLTEGSPVWASVKATQIDTYPAHDG"
     gene            complement(1953..3200)
                     /locus_tag="VFD74_05970"
     CDS             complement(1953..3200)
                     /locus_tag="VFD74_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HZK49132.1"
                     /translation="MRWNLTSDRVPQAWFNVAPHLPEPLQPPLHPGTREPIGPDDLTP
                     LFPMALIGQEMSAEPWIDIPGEVMDILRLWRPTPLVRARRLEEALDTPARIYYKDESV
                     SPAGSHKPNTAVPQAFYNKAEGIGRLTTETGAGQWGSALSFASALFGLECKVYMVRAS
                     YQQKPYRRVMMETWGGSVEPSPVDDPDQPGSLGSAISDAVRDAVSRDDSHYSLGSVLN
                     HVLLHQTVIGLEAKEQLALAGEERPDIVIACCGGGSNLGGLAFPFVPDADVELLAVEP
                     SSCPTLTEGRFDYDFGDTAGLTPLLAMYTLGHDFIPPSIHAGGLRYHGVSPIISNLVK
                     AGRMRAVAYPQGKVFDAAVQFARTQGTIPAPETSHAIRATIDEALRAKEEGVERVILF
                     GYSGHGLLDLSAYDDYFHGRLIE"
     gene            complement(3249..3707)
                     /locus_tag="VFD74_05975"
     CDS             complement(3249..3707)
                     /locus_tag="VFD74_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK49133.1"
                     /translation="MDLGVTDVEVASLLRLVRIPFGAGSIALAAAVASIDAGPELAAR
                     VAMVQAERDRVATQLTGLGWEIPSSQANFLWIAAGEAQATAAACLCAESKVLVRQLPG
                     GLRVTIGTEKENARLLDALGSPADLPPTRSAAGGSASRADPCSSSPLISS"
BASE COUNT          639 a         1299 c         1274 g          604 t
ORIGIN      
        1 cgggcgcgtg cggatgtcgc gggcgaggta ggaggccagc acgtgcgagg cgacgatggc
       61 gagaaacttg gggcgccgca gctccacgtt cgcaccggag attggctctg ggcgcactgc
      121 aaccgcgaag cgagtccccc cggcgccatg cacgtcaacg gcgacctcac cggcgcgccc
      181 ggccgccagc gcgtcgagga gaccggggag ctcgtcgggg atgccggcac ctaccaccac
      241 gacgtcgacg ccggcgagca tggcgccgta cgtcgccgcc ggggtggcca tctggagctt
      301 ctccaggtag ttgaccccta ccaggccgtc gtggccctcc ttggcgagga acacctccac
      361 gaagttggcc gcgatgacga gatcggtggc ccgccgactc ggatgcagcg acagtcgcgg
      421 taccgagcgg aacggctcct cggggccgat gccacctgcg acgaagtagc gctccacgat
      481 ctgctccgag acgtcggcgt acgggaagct ggtgagcgcc cggcgggtat gacctcccgg
      541 gtcgcccagc tggaggcggc gggcgatgag ggtgtccagt gccactcccg agaccacccc
      601 gagctggcct gcaatcgcga ctgcccgtgc caagccccaa cccgagacgc cgacgcccat
      661 gccgccctgg attatcgccg gccagtcact ccccgcccgc ggacccctca ccgtcgatcc
      721 tcggtcggag ttccatgcac tttgtgccca cctcggagct tgaagtggcc gtcactgact
      781 tgcatgcccg aagcaggtcg atccacatgc gagagactag tcgtctctac cactatacgt
      841 agagggctgg cctttacgtc gatgccttca acgagagtga cgcagagcgt gggccgcctc
      901 caccattgcc caacgtacga ggcgcgaatc cagcttcgca atcggtcccc agtgcccggt
      961 gcgccgctgt atcggactca tggtgccggg gccgtgagcc ctgcccggct cctcctgctc
     1021 gacgagccca tggctgccct cgacctgggt acccgcggat cggtccgcca ccatctgcgg
     1081 cgccacctcg ccgagatcac cgggtcgtgc gtgttggtga cccacgatcc cctcgacgcc
     1141 gccgccatcg ccgatcggct cgtcatcatc gaggccggga ggctggccca acaagggacc
     1201 ctgaaggaga tcacgacccg tccccgcacc gcgtacatcg ctggtctgat gggcctcaac
     1261 ctgctcagcg cccgcgcacg tgggacgacc ctcaccctgg ccaacggcgc caccctggaa
     1321 gcggccagtc cgacgaccgg cgaggtcttc gtggtcatcg caccccagag catcgccctg
     1381 tacccggggc gacccgaggg gagccctcgg aacacgtggc cgacccgggt gagcgaaatc
     1441 cacctcatgg gcgatcgggt acgggtgctt gtcgacgaac cggtccccgt gaccgccgag
     1501 atcaccgccg tctcgctggc ggagctcggt ctgaccgaag ggtcaccggt gtgggcctcg
     1561 gtgaaagcca cgcagatcga cacgtatccc gcgcacgacg gatagccacc gccagagaac
     1621 agtgacaatt tcgaggacat cttcggggta tctcatctac cgggtcccga tcggcccccg
     1681 tccgctcgaa tgcctgccct gaccggtcac aacgcgccga gcaggaaaga cgtacccctg
     1741 taccagacag cacggctctc aaggccccga caagctgacc cgaccaacac ggggcagggc
     1801 tggttttcgc aggcgaggcc ggcccgttgg ctggccgcgg gcacggcgtg cccaggtgcg
     1861 tcggcgacta cgtcagcaat ggcggcgatc ctcctgaccg gcggcgacac cggcgggacg
     1921 cggaggcagc gcgtcgtact gatgggtcgc cgtcactcga tcagcctgcc gtggaagtag
     1981 tcatcgtatg cgctgagatc gagcagcccg tggccggaat agccgaagag gatcacccgc
     2041 tccaccccct cctccttcgc ccgcagggct tcgtcgatgg tcgcccggat cgcgtggctc
     2101 gtctccgggg ccgggatcgt gccctgggtg cgggcgaact ggaccgcggc gtcgaacacc
     2161 ttgccctggg gataggcgac ggcacgcatg cggcctgcct tcacgaggtt ggagatgatc
     2221 ggggagacgc cgtggtaacg gaggcccccg gcgtggatgc tcggcgggat gaagtcgtga
     2281 ccgagggtat acatggcaag gagcggggtc aggcctgccg tgtcgccgaa gtcgtaatcg
     2341 aaccggccct ctgtgagcgt tgggcacgac gacggctcga ctgcgaggag ttcgacgtca
     2401 gcgtcgggca cgaaggggaa cgcgaggccc ccgaggttcg agcctccacc acagcacgcg
     2461 atgacgatgt cgggacgttc ctccccagcc agtgctagtt gctcctttgc ctcgaggccg
     2521 atcacagtct ggtgcagcag cacgtgattg agcacagacc cgagcgaata gtgggagtca
     2581 tcccgggaga cggcatcccg gacggcgtca ctgatggcgg acccgagcga gccaggttgg
     2641 tccggatcat ccaccggaga gggttcaacg ctgccgcccc aggtctccat catcacccgg
     2701 cggtagggct tttgctggta agaggcacgg accatgtaga ccttgcactc gagcccgaag
     2761 agcgcgctcg cgaacgagag cgcgctcccc cactgtccgg cacctgtctc ggtggtgagg
     2821 cgcccgatcc cttccgcctt gttgtagaac gcctgcggca ccgcggtgtt gggcttgtga
     2881 gagcccgccg gggagaccga ctcgtccttg tagtagatcc gggcgggcgt gtcgagcgcc
     2941 tcctcgagcc ggcgcgcccg tacgagcggc gttgggcgcc agagccggag gatatccatc
     3001 acctcacccg ggatgtcgat ccagggctct gccgacatct cctggccgat gagcgccatc
     3061 gggaacagcg gcgtgaggtc gtccggcccg atcggctcgc gcgtccccgg gtggaggggc
     3121 ggctgcagcg gctcgggaag gtgtggcgcg acgttgaacc acgcctgcgg cactcggtca
     3181 gatgtgaggt tccacctcat ggtcgtgctc ctttgatatc ttcgatggga cgggactgct
     3241 ggcggtggtc aactactgat caacggactg ctcgaacacg ggtcagctcg gctggccgac
     3301 ccccctgccg ccgagcgcgt cggcggcaga tcagcaggag agcccagggc atcgagcagc
     3361 cgggcgttct ctttctcggt gccaatggta acccgcagcc cgcccggtaa ctggcggacg
     3421 aggaccttgc tctcggcaca gaggcacgct gctgcggtcg cttgagcctc tcccgcggcg
     3481 atccaaagga agttggcctg cgatgaaggg atctcccatc ctagcccggt gagctgagtc
     3541 gcgaccctgt cgcgctccgc ttgtaccatc gccacccggg cggccagctc gggaccggcg
     3601 tcgatggagg cgactgctgc agccagggcg atgctaccgg cgccgaaggg gattctcacc
     3661 agacgaagca gcgaggccac ctcaacatcg gtgacgccca gatccacctg tcgcaatcgg
     3721 cgatgtggtg ataatcaggt gccccatctg ctcgggtctg accctgggtg cgactgaccc
     3781 ctttttgggc tccgcgctag tgcgtgacat ctaaat
//
LOCUS       DASRMJ010000218        34855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_305830, whole genome shotgun sequence.
ACCESSION   DASRMJ010000218 DASRMJ010000000
VERSION     DASRMJ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 34855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34855
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305830"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..343)
                     /locus_tag="VFD74_05980"
     CDS             complement(56..343)
                     /locus_tag="VFD74_05980"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZK49134.1"
                     /translation="MRGVVTIGPTSPVQMQGESGEAPYEATITVTNSSGATVAEVRSS
                     EDGRFSVDLAPGRYTLVPQSPGPLPRADPYDVAVEPHAFTEVSIQYDSGIR"
     gene            complement(449..1066)
                     /locus_tag="VFD74_05985"
     CDS             complement(449..1066)
                     /locus_tag="VFD74_05985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZK49135.1"
                     /translation="MRVRSLPLRDQAGTIVGAAEIFAPDRDAPEAARRIAELEELAYL
                     DALTALPNRRYLQSQLEQRLGELQRYGWPFGAVLLDVDHFKNVNDVFGHPTGDRVLTM
                     VAKTLELATRPFDLVGRWGGEEFLAVVTNIDLESLTAVADRFRALVRTSSLTDTPSPI
                     SVTVSAGATLANLEDDPESILQRVDRLLYRSKEEGRDRTSVEQPG"
     gene            complement(1074..1406)
                     /locus_tag="VFD74_05990"
     CDS             complement(1074..1406)
                     /locus_tag="VFD74_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZK49136.1"
                     /translation="MPNGPVGSTEEDVGKIPRDPRLMGSLLDDLDEGVYFTDRDRRVL
                     YWNRGAEAITGFPASRVVGSRCRDNLLMHVDEEGTQLCMGGCPLEATMTDGDPREAHV
                     FLHHADGQ"
     gene            1638..1722
                     /locus_tag="VFD74_05995"
     tRNA            1638..1722
                     /locus_tag="VFD74_05995"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1672..1674,aa:Tyr,seq:gta)
     gene            1768..1843
                     /locus_tag="VFD74_06000"
     tRNA            1768..1843
                     /locus_tag="VFD74_06000"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1801..1803,aa:Thr,seq:ggt)
     gene            1862..1935
                     /locus_tag="VFD74_06005"
     tRNA            1862..1935
                     /locus_tag="VFD74_06005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1896..1898,aa:Met,seq:cat)
     gene            2094..2243
                     /gene="rpmG"
                     /locus_tag="VFD74_06010"
     CDS             2094..2243
                     /gene="rpmG"
                     /locus_tag="VFD74_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZK49137.1"
                     /translation="MRVRVTLACQECKRRNYTTMKNKRNDPERLEFQKFCKWCGSHTS
                     HKETK"
     gene            2299..2374
                     /locus_tag="VFD74_06015"
     tRNA            2299..2374
                     /locus_tag="VFD74_06015"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2332..2334,aa:Trp,seq:cca)
     gene            2404..2691
                     /gene="secE"
                     /locus_tag="VFD74_06020"
     CDS             2404..2691
                     /gene="secE"
                     /locus_tag="VFD74_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZK49138.1"
                     /translation="MARTTTTKTSAKVPAKAVAAPKTTKPVAGAAPSRRGRPTRFLRE
                     VRTEMSKVTWPPRQELVQATMVVIVAVAIAASYIGVFDLIWSSLVNLARLG"
     gene            2726..3253
                     /gene="nusG"
                     /locus_tag="VFD74_06025"
     CDS             2726..3253
                     /gene="nusG"
                     /locus_tag="VFD74_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZK49139.1"
                     /translation="MLAKKWYVINTYSGHENKARANLEHRIQSMDLAPYFEDIVVPTE
                     VVVETKGGEKVQTEKRIFPGYVLVKMNLTDDSWSLVKNTPGVTGFVGSENKPTALSPA
                     EVDRILHTSTAEKPKAKAEFSVGDQVKVTSGPLAEFTGEVAEVNIEQSKLKVMVSIFG
                     RDTPTELGFEQVKRI"
     gene            3291..3719
                     /gene="rplK"
                     /locus_tag="VFD74_06030"
     CDS             3291..3719
                     /gene="rplK"
                     /locus_tag="VFD74_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZK49140.1"
                     /translation="MAKKVKANIKLQIPAGQANPAPPVGPALGQHGVNIMEFCKAYNA
                     QTQDKIGTIIPVEITVYEDRSYSFILKTPPAAVLIRQAAGVPKGSGVPNRDKVGRITR
                     EQLRQIAEVKLPDLNANDVEAAMNILAGTARSMGVTVEKK"
     gene            3859..4578
                     /gene="rplA"
                     /locus_tag="VFD74_06035"
     CDS             3859..4578
                     /gene="rplA"
                     /locus_tag="VFD74_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZK49141.1"
                     /translation="MSKGSKRFEEAKRAVETGRVYLPGDAIRLIKSLETANFDETVEV
                     HIKLGVNPRHAEQQVRGTIMLPNGTGKTIRVAVFAKGDKAAEAEAAGADVVGAEDLAA
                     RVEQGFLDFDAAIATPDMMGVVGKLGRVLGPRGMMPNPKAGTVTFDVGKAVQDMKAGK
                     IEYRTDKFGVVHVIIGKKSFGERELIENYAEFIDELVRVKPSAAKGKYLRSITFASTM
                     GPGVPVDTTKTRGLLEDEATA"
     gene            4839..5360
                     /gene="rplJ"
                     /locus_tag="VFD74_06040"
     CDS             4839..5360
                     /gene="rplJ"
                     /locus_tag="VFD74_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZK49142.1"
                     /translation="MAKPEKVAAVDRIIEKLRSTDVYYLVDFRGLSVGDATDLRSRLR
                     ANGADLIVVKNTLAKRAAAEAGIEGLDGLFEGPTAIAYCGDDPVAPAKTLVGFVKEKK
                     KLVIKGGFLQSQILLQAQVEQLAQLPSREELIAKVVGGIAAPLYGLANVLTGPIRGLV
                     VALDQIREQKAAA"
     gene            5430..5798
                     /gene="rplL"
                     /locus_tag="VFD74_06045"
     CDS             5430..5798
                     /gene="rplL"
                     /locus_tag="VFD74_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZK49143.1"
                     /translation="MTPQELIEEIKKMTVLELAEVVKALETEFGVTAAAPMAAAAAPA
                     AAVEEVEEQTEFDVILSAVGEKKIQVIKVVRALTGLGLKEAKDLVDGAPNPVKQGVTK
                     AEAEELKKQLEEAGAAVEIK"
     gene            5961..9548
                     /locus_tag="VFD74_06050"
                     /pseudo
     CDS             5961..9548
                     /locus_tag="VFD74_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
     gene            9783..14105
                     /locus_tag="VFD74_06055"
     CDS             9783..14105
                     /locus_tag="VFD74_06055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZK49144.1"
                     /translation="MIDINEFDAIKIGLASPEQIRAWSSGEVTKPETINYRTLKPEKD
                     GLFCERIFGPTKDWECYCGKYKRVRYKGIICERCGVEVTRAKVRRERMGHIDLAAPVS
                     HIWYFKGVPSRMGYLLDISPKRLERVLYFSASIVTHIDEAKREADKEKLEELIQADID
                     NLFTARDERKARILAERDKRVSVIMGNSTPDEDDQAALGLDEVPIAEWTKKEITKRSA
                     ELREHADLALRELERYAEEQAERTREMWAAFRNLKQGEIITDETFFRDLKEKFGNPFG
                     YGIYFKGGMGAEAIRELLRAVDLETEATSLREIIKTSKGQRQSRAVKRLKVVEGFLRS
                     DNRPEWMVLEAIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAP
                     EIIVNNEKRMLQEAVDALFDNGRRGRAVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKR
                     VDYSGRSVIVAGPELKLHQAGIPKTMALELFKPFVMSRLVERRQVQNIKAAKKIVESE
                     SPEVWDILEEVITEHPILLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCTAFNADFD
                     GDQMAVHLPLSVEAQSEARILMLSSNNILSPAHGRPIAVPSQDMILGVYYMTYYEPLE
                     GQPELEAVDVADPEALHALDPQPRVFSEVAELEHAYLDHNVGLQDPILLRWDGQRIMT
                     TVGRAMFNSAVVTSLAGYFGDAWAGGPDGGPGRKDDYEYRNRVFTKKVMTDFISDLVE
                     TFGATAVAHLLDVLKDLGFRYATVAGVTISKNDIVVPPEKEQILQGYEEKVARIQKEY
                     MRGNLTEVERRERVTDLWTEATDKVAEAMEENFRRLNPVYMMANSGARGSFKQIRQLA
                     GMRGLMANPKGEIIERPIKANFMEGLSVLEYFISTHGARKGLADTALRTADSGYLTRR
                     LVDVAQDVVIREVDCGTTESIVLPYRRDDKSEHLLGRLFGENIVDPETGEVLHQRNDT
                     ITKRALRDLDKVFPADEHKEMKLKVRSVLVCESEFGVCQRCYGRSLATNELVAIGEAV
                     GHVAAQSIGEPGTQLTMRTFHTGGVAGADITHGLPRVVELFEARKPKGQARIVDAELA
                     PEREYFVEFDDPANPRRIQIVDDLGEEAASYPVSRRMQLMVNHGDRVYAGDQLYQGPL
                     NPHDVLQHRGILGTGQYLVSEVQKVYKSQGVDISDKHIELIARQMTKRVRVDACGDTH
                     FLPGQTVDKIAFLRENEAAVAVGEEPATFEVMIMGITKASLATESFLSAASFQETTKV
                     LTDAALEGKTDHLRGLKENVIIGKLIPAATGLRRYRNIEVNPVRTIRPDVEGFFGAGA
                     GQTDVGRSGTLTTEQALAMLGGAGDGWADASVDDLDLPTYVHSILTGHGIEALAALLE
                     RTPEELLGISGFGQKSLEEVRNRLADRGLALKGDEVEGVDQSLTDDDQDDFEMSVSED
                     TQED"
     gene            14239..14610
                     /gene="rpsL"
                     /locus_tag="VFD74_06060"
     CDS             14239..14610
                     /gene="rpsL"
                     /locus_tag="VFD74_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZK49145.1"
                     /translation="MPTINQLIRKGRAELAKKSKTPALKGAPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTAYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYKVIRAAL
                     DTAGVANRKQSRSKYGVKAPK"
     gene            14630..15100
                     /gene="rpsG"
                     /locus_tag="VFD74_06065"
     CDS             14630..15100
                     /gene="rpsG"
                     /locus_tag="VFD74_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZK49146.1"
                     /translation="MARRIKAQKREIQPDPVYRNRLVTQITNRLMIDGKKSTAEHIVY
                     GALDIMRERTGQDPIAVLKEAVENVRPALEVKSRRVGGANYQVPVEVATRRSYTLAVR
                     WFITHSRVRREKSMSERLAGELLDGFNRTGVTMKKKEDLLRMAEANKAFAHYRW"
     gene            15296..17371
                     /gene="fusA"
                     /locus_tag="VFD74_06070"
     CDS             15296..17371
                     /gene="fusA"
                     /locus_tag="VFD74_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZK49147.1"
                     /translation="MFPLERVRNIGIMAHIDAGKTTTTERILYYTGRTYKIGEVHDGA
                     AVMDWMQQEQERGITITAAATTCEWKRHRVNIIDTPGHVDFTMEVERSLRVLDGAVAV
                     FDAVAGVEPQSETVWRQADKYRVPRIAFINKMDRTGADFGQAVQTMIDRLHARPMVVQ
                     LPVGAEDGFRGIIDLVRMRAYLFQDDMGVEWEDAEIPADLVDEADIARAHMIEILADY
                     DEQIMHAYLEGDEVSEAALLAAIRTSTLQIGATPVLCGSAFKNKGVQMLLDGVIDYLP
                     SPLDIPPMVGFDPRTDAEITRLASDDEPFSALAFKIMTDPFVGKLTFFRVYSGVLKAG
                     SYVYNASKGKKERVGRILQMHANHREDRSEIFAGDIAAAVGLKATTTGDTLTDPDYPV
                     ILESIEFPEPVISVAIEPKTKLDQDRLGESLMKLAEEDPTFRVHNDEETGQTIIAGMG
                     ELHLEIIVDRLLREFKVDANVGRPQVAYRETIRAVVEKAEGRFVRQTGGRGQYGHVLL
                     RMSPAEQGAGFTFTNKVVGGSVPREYVAPTQAGIVEAMSGGVLAGFPMVDIDVELYDG
                     SYHDVDSSEMAFKIAGSMAFKAGAEKGKPVLLEPIEDVEVVVPEEYMGDVIGDLNSRR
                     GHILGMEPRAGAQVVRAQVPLSEMFGYATDLRSSTQGRATYTMQFIHYAEVPASIATE
                     IVARFRGDK"
     gene            17441..18646
                     /gene="tuf"
                     /locus_tag="VFD74_06075"
     CDS             17441..18646
                     /gene="tuf"
                     /locus_tag="VFD74_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZK49148.1"
                     /translation="MAKKKFERTKPHVNVGTIGHVDHGKTTLTAAITACLAARNPNVT
                     VRSFDSIDNAPEERERGITIATAHVEYETANRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVSAADGPMPQTREHILLARQVGVPYIIVFLNKADMVDDPELLELVEMEVRELLT
                     EYEFPGDDIPVITGSALKALECACGGDECEACKPILELMKAVDEYLPEPERDTEKPYL
                     MPIEDVFTIQGRGTVVTGRIERGIVKVGDDVQIVGMQKEVVKTVCTGVEMFRKLLDEG
                     RAGDNVGVLLRGIKRDDVQRGMVLAKPGSITPHTEFRAEVYVLTREEGGRHTPFFNGY
                     RPQFYFRTTDVTGDSTLDEGVEMIMPGDNTNLTVKLITPIAMEEGLRFAIREGGRTVG
                     AGVVTKVIQ"
     gene            18723..19031
                     /gene="rpsJ"
                     /locus_tag="VFD74_06080"
     CDS             18723..19031
                     /gene="rpsJ"
                     /locus_tag="VFD74_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZK49149.1"
                     /translation="MAQQKIRIRLKAYDHELIDKSARSIVETAERTGATISGPVPLPT
                     EKNVYCVIRGPFKDKDSREHFEIRTHKRLIDIHQPTPKTVDSLMRMDLPAGVDIEIKL
                     "
     gene            19028..19639
                     /gene="rplC"
                     /locus_tag="VFD74_06085"
     CDS             19028..19639
                     /gene="rplC"
                     /locus_tag="VFD74_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZK49150.1"
                     /translation="MMKALLGTKIGMTQLFGEDGVVTRVTVIQAGPCVVTQKKDVASD
                     GYAATQIAFGDVKVKKLTRPELGHLKKSGASPKKHLAEVRGEHDLAVGDRVTVEAFAP
                     GDMVRVTGVSKGKGFQGVIKRHGFKGGPASHGSRFHRAPGSIGASADPARVYPGTKLP
                     GQMGNQRVTQTGLTVVAADPERNLLMVKGSVPGSKGSLVFVRG"
     gene            19644..20267
                     /gene="rplD"
                     /locus_tag="VFD74_06090"
     CDS             19644..20267
                     /gene="rplD"
                     /locus_tag="VFD74_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZK49151.1"
                     /translation="MPTIRKIDEKGAVIGEHEFADSRVETEMNIGLLHEVVRGEQAAR
                     RQGTHAAKTRGQVSGTGAKAFRQKGTGRARAGSTRVSQWTGGGFAFPPVSRDHSFKVN
                     KKVRVKAFRMALGNLIANDAVRVLTGVEFEVPSTKRASAILDSAALDGPTLVVAASQE
                     MNTLLSFRNLPRTRVLPVNEVDVQDYLWARSAVLTEAAMAILEGGAN"
     gene            20267..20551
                     /gene="rplW"
                     /locus_tag="VFD74_06095"
     CDS             20267..20551
                     /gene="rplW"
                     /locus_tag="VFD74_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZK49152.1"
                     /translation="MDARQVIIRPVISEKSYEAVEKGRYSFEVHTKATKQHIRDAVEE
                     IFKVSVIGVNTMNVKPKPKRRGAFKGTTRRWKKAVVELAPGNRIEFFGGN"
     gene            20577..21407
                     /gene="rplB"
                     /locus_tag="VFD74_06100"
     CDS             20577..21407
                     /gene="rplB"
                     /locus_tag="VFD74_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZK49153.1"
                     /translation="MAIKSYKPTSPGRRFTTTPAFDEITKTEPEKSLIVPVKRKGGRN
                     NNGRITTRHQGGGHKRMYRIIDFKRDKDGVPGRVSSIEYDPNRSCRIALIVYADGEKR
                     YILAPNKLKVGDQVEAGPGADIKVGNALPLKNIPVGSTVHNIELHIGKGGQIARAAGT
                     SAQVMAKEGNYATLRLPSGEMRMIHLACRATVGEIGNSEHENLSTGKAGRTRWMGIRP
                     TVRGTTMNPVDHPHGGGEGSTTPGRHPVTPWGVPTLGHKTRGTKASDKYIIRRRGKGR
                     "
     gene            21416..21697
                     /gene="rpsS"
                     /locus_tag="VFD74_06105"
     CDS             21416..21697
                     /gene="rpsS"
                     /locus_tag="VFD74_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZK49154.1"
                     /translation="MTRSSKKAPFVAEKLMLRIERMNAAGEKRMLKTWSRASTIYPEM
                     VGHTIAVHDGRKHVPIYVTESMVGHKLGEFALTRTYRGHGGGKEKRSRR"
     gene            21701..22039
                     /gene="rplV"
                     /locus_tag="VFD74_06110"
     CDS             21701..22039
                     /gene="rplV"
                     /locus_tag="VFD74_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZK49155.1"
                     /translation="MVSATAKYVRLSPRKARQVVDLIRGKSVRDAKAILMLTPRGAAP
                     IVQKVLASAVANAENNNDMSADDLYVANAYVNEGPTLKRFKPRAMGRATPIRKRTSHI
                     TVAVDERRES"
     gene            22043..22774
                     /gene="rpsC"
                     /locus_tag="VFD74_06115"
     CDS             22043..22774
                     /gene="rpsC"
                     /locus_tag="VFD74_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZK49156.1"
                     /translation="MGQKVHPGGMRLGIIHDWKAHWFTEKNFADFLQEDLAIRKYIRR
                     RMGHAGLSEIVIRKDTNKVIIDIHTARPGIVIGKSGAEVDGLRRDIHGMTGKNVHVNI
                     VEIKRPELDAGLVAQSIAEQLANRVSFRRAMKRAITSAMRSGAAGIKVRSGGRLGGAE
                     MARVEGYSEGRVPLHTLRADIDYGFWEANTTFGKIGVKVWINKGEIMPEGFDSDARKE
                     PALGVPEERRGGRSGRGRRGEGSRW"
     gene            22870..23295
                     /gene="rplP"
                     /locus_tag="VFD74_06120"
     CDS             22870..23295
                     /gene="rplP"
                     /locus_tag="VFD74_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZK49157.1"
                     /translation="MLLPKRIKHRKQQRGRMGGKSKGQTTVQFGEFGLQALEPGWVTA
                     RQIESARIAMTRKMKRGGKVWINIFPHKPITKKPAETRMGSGKGSPDQWVVVVKPGRV
                     MFELAGVPEDLAREAIRLAVHKLPLKCRFVVREDGSFEG"
     gene            23285..23482
                     /gene="rpmC"
                     /locus_tag="VFD74_06125"
     CDS             23285..23482
                     /gene="rpmC"
                     /locus_tag="VFD74_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010018524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZK49158.1"
                     /translation="MKAKDIIELAEHELEQRLKESREELFNLRFQHATGQLENTGRLR
                     QVRQDIARILSVQNSQKSKES"
     gene            23483..23746
                     /gene="rpsQ"
                     /locus_tag="VFD74_06130"
     CDS             23483..23746
                     /gene="rpsQ"
                     /locus_tag="VFD74_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZK49159.1"
                     /translation="MTAERESSQRERRGVVISDKMEKTIVVEVAVAKPHPLYKKIVRR
                     SSRLKAHDENNEARIGDVVLVRECRPLSKSKSWRLISILERAE"
     gene            23778..24146
                     /gene="rplN"
                     /locus_tag="VFD74_06135"
     CDS             23778..24146
                     /gene="rplN"
                     /locus_tag="VFD74_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZK49160.1"
                     /translation="MIQVETRLKVADNTGARELLCIRIRGGSHRRYAGVGDIIVGTVK
                     EATPGGSVKKGDVVQAVVVRVKKSYGRKDGTYIGFDENAAVLIDRQKNPRGTRIFGPV
                     ARELREKNFAKIVSLAPEVL"
     gene            24146..24484
                     /gene="rplX"
                     /locus_tag="VFD74_06140"
     CDS             24146..24484
                     /gene="rplX"
                     /locus_tag="VFD74_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZK49161.1"
                     /translation="MKKQAPRVRKIRKGDTVEVIQGKERGKRGKVLRVLAEDQRVVVE
                     RINFIKRHVRPSQKNPQGGVIEREAGIHVSNVKLVCPSCDAAVRVGMREERGDKVRYC
                     KKCNVQVDKA"
     gene            24487..25035
                     /gene="rplE"
                     /locus_tag="VFD74_06145"
     CDS             24487..25035
                     /gene="rplE"
                     /locus_tag="VFD74_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZK49162.1"
                     /translation="MAIPRFREKYTSQVVPELLSEFGYSNIMQVPRIQKITVNMGVGE
                     AAQDAKLLEEAAKQLGQITGQKAQVRRSRKSIANFKIREGMPVGLRVTLRGDRMWEML
                     DRLISIALPRIRDFRGISPKGFDGQGNFNLGLKEQIIFPEIDYDKIDRVRGLDVAITT
                     TAETDDEARALLKKLGMPFRES"
     gene            25053..25238
                     /locus_tag="VFD74_06150"
     CDS             25053..25238
                     /locus_tag="VFD74_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZK49163.1"
                     /translation="MAKKSLIAKANRTPKFSSRAYNRCRRCGRPRGYFRRFGLCRICL
                     RELAHEGVIPGVTKSSW"
     gene            25250..25648
                     /gene="rpsH"
                     /locus_tag="VFD74_06155"
     CDS             25250..25648
                     /gene="rpsH"
                     /locus_tag="VFD74_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZK49164.1"
                     /translation="MSQSDPVADMLTRLRNANKALHSEVSMPTSHLKVEVARILKDEG
                     YIAGYKVVKGESYDTLLVDLKYTEDRRRVITQLKRISKPGRRIYAKKDKLPKVLGGLG
                     MAVVSTSRGVMSAREAQRLGVGGEVICFVW"
     gene            25655..26188
                     /gene="rplF"
                     /locus_tag="VFD74_06160"
     CDS             25655..26188
                     /gene="rplF"
                     /locus_tag="VFD74_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZK49165.1"
                     /translation="MSRIGREPINVPAGVEVKIDGQHVTVKGPKGLLSRDLNPAVDVQ
                     LADGVISVTRPSDSATHRSLHGLTRTLVANMVIGTTTGFEKRLNIVGVGYRAAMKGTD
                     LEVQAGYSHPVVVNPPDNIEFEVPVPTLIVVKGIDKQQVGQVAANIRAIRKPEPYKGK
                     GIRYQGEVVRRKVGKRA"
     gene            26209..26562
                     /gene="rplR"
                     /locus_tag="VFD74_06165"
     CDS             26209..26562
                     /gene="rplR"
                     /locus_tag="VFD74_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZK49166.1"
                     /translation="MSRIAKIKRASDRRAKRVRGGVRGTTERPRLTVFRSNRGIWAQV
                     IDDMNGTTLASAGSINIAEKLPKREMAAKVGELLAERAKAAGVARVVFDRGPYLYHGR
                     VKALADGAREGGLEF"
     gene            26581..27126
                     /gene="rpsE"
                     /locus_tag="VFD74_06170"
     CDS             26581..27126
                     /gene="rpsE"
                     /locus_tag="VFD74_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZK49167.1"
                     /translation="MATIKAEALNLQENVIEINRVAKVVKGGRRFSFTALVAVGDGES
                     IVGVGYGKAKEVPLAIQKAIDDAKKNLFVVPKHGSTITHGVTGRFGAGRVLLRPASPG
                     TGVIAGGGVRAVLELGGVHDVLTKCLGTTNPINMVRAVEQGLRSLRRPEEIAALRGKT
                     VREVLGMEKREHPASTEIAGE"
     gene            27132..27323
                     /gene="rpmD"
                     /locus_tag="VFD74_06175"
     CDS             27132..27323
                     /gene="rpmD"
                     /locus_tag="VFD74_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZK49168.1"
                     /translation="MLAITQTRSGIRRKHDHKATLRALGISRMGQTVYHDDNPVIRGM
                     VKKIEYMLEVAEVDVKDEG"
     gene            27336..27800
                     /gene="rplO"
                     /locus_tag="VFD74_06180"
     CDS             27336..27800
                     /gene="rplO"
                     /locus_tag="VFD74_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZK49169.1"
                     /translation="MFLHNLRPTEGSRSKKKRVGRGEGSGKGKTSGKGHKGANARSGG
                     GVSARYEGGQMPLHMRLPKLRGPLAKTSMAIGPFRTFMTPVNISRLAAFPAGAEVTPE
                     ALVEMGIIKKADERVKILADGELDRALTVRAHGFSAAAKTKIEAAGGTAEVL"
     gene            27844..29097
                     /gene="secY"
                     /locus_tag="VFD74_06185"
     CDS             27844..29097
                     /gene="secY"
                     /locus_tag="VFD74_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZK49170.1"
                     /translation="MFQSLLNAWRLPDLRKRLVFTAFIIAMYRLGSFIPVPGVDLQAI
                     KDLVSGGGVFAFLDLFAGGALERVAVFALGIMPYITASIIMQLLQIVIPQLEALAKEG
                     ESGAKKINQYTRYFTVVLALIQSTGYVIIFKSQGAFTTANPLNPLRFYLIVMTLTVGT
                     ALVMWLGELITQRGIGNGISLIIFASIVSRLPSGLYKMFSTMNPLEWLAMALIALGVI
                     AAIVLIQEGQRRIPVQYAKRVVGRKMYGGQSTYLPLRVNMAGVIPVIFASAILVFPVT
                     LAQMTPWGWAQSAARAVGPGTGMYLVAEVLLIIIFTYFYTAVQFNPLDQADNLKKYGG
                     FIPGIRPGRPTAQYLDKVLTRITLPGAVFLASIAALPTILIWVMGIPFFFGGTSLLIV
                     VGVALDTMKQMESQLLMRHYEGFLK"
     gene            29116..29772
                     /locus_tag="VFD74_06190"
     CDS             29116..29772
                     /locus_tag="VFD74_06190"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZK49171.1"
                     /translation="MAQFDLILLGPPGAGKGTQAGRIVEDYSIPHISTGDMLRAAVKN
                     QTAMGLEAKRYMDAGDLVPDSVVIGIVKDRLQEPDTAGGFLLDGFPRTIPQAEALDDM
                     LESIERGVSKALAILVDEEALVRRLSGRRICRDCQTPFHMEFGPPSVAGVCDKCGGEL
                     YQRDDDSEATVRNRLAVYGRQTEPLIAYYDSQSVLARIDGDQTPDEVYEDLKEAIGRP
                     "
     gene            29783..30526
                     /gene="map"
                     /locus_tag="VFD74_06195"
     CDS             29783..30526
                     /gene="map"
                     /locus_tag="VFD74_06195"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZK49172.1"
                     /translation="MIIRKTPEEIEAIARAGRILRGCLDVLSQKVEPGVSTKELDRLA
                     EEFIRGHGGTPTFKGYRGFPGSICTSPNDMVVHGIPGSTRLREGDIVGIDVGVTLDDF
                     IADAAVTLAVGDVSEEAQALMTATRESLMLAVEECWPGKRIGDISHAVQSHVEVRGYS
                     VVRSLVGHGVGRAMHEDPQVPNFGLPGRGPRLSEGMVLAIEPMINSGGFDVEVGADSW
                     SIFTKDGGLSAHFEHTVAVTAAGPRVLTA"
     gene            30691..30909
                     /gene="infA"
                     /locus_tag="VFD74_06200"
     CDS             30691..30909
                     /gene="infA"
                     /locus_tag="VFD74_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZK49173.1"
                     /translation="MPKEDAIELEGEVVEALPNTMFRVKLENDHEVLAHISGKMRMNY
                     IRILPGDKVKVELSPYDLTRGRITYRFK"
     gene            31171..31284
                     /gene="rpmJ"
                     /locus_tag="VFD74_06205"
     CDS             31171..31284
                     /gene="rpmJ"
                     /locus_tag="VFD74_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZK49174.1"
                     /translation="MKVRASVKPMCEKCKVIRRKGVVLVICQNPRHKQRQG"
     gene            31298..31672
                     /gene="rpsM"
                     /locus_tag="VFD74_06210"
     CDS             31298..31672
                     /gene="rpsM"
                     /locus_tag="VFD74_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZK49175.1"
                     /translation="MARIAGVTLPREKRVEIGLTYVYGIGRSRSNVVLAAVGVDPNTY
                     VKDLTEDEEIRLRAYIEENVIVEGDLRRERSQNIKRLMEIGCYRGIRHRRGLPVRGQR
                     TKTNARVRKGHKPTVGVKKKVR"
     gene            31721..32125
                     /gene="rpsK"
                     /locus_tag="VFD74_06215"
     CDS             31721..32125
                     /gene="rpsK"
                     /locus_tag="VFD74_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006363202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZK49176.1"
                     /translation="MAKPVKHGGGRVRRRKVRKNIAVGNAYIKTSFNNTMVTLTDKEG
                     NVISWASAGVAGFKGSRKSTPFAAQVTAEKAARAGMEHGLQKVDVFVKGPGSGRETAI
                     RSLQAAGLEVGGVKDCTPIPHNGCRPKKRRRV"
     gene            32144..32770
                     /gene="rpsD"
                     /locus_tag="VFD74_06220"
     CDS             32144..32770
                     /gene="rpsD"
                     /locus_tag="VFD74_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZK49177.1"
                     /translation="MARDTSAQCKQCRREGIKLLLKGERCLTDKCAIDRRGYPPGEHG
                     RGRRRKQSEYLVQLREKQKARRYYGVLEKQFHNYYEAAHRQTGVTGENLLRLLELRLD
                     NVVYRLGFGASRRQARQLILHGHFLVNGKRVNIPSYKVRPDEIITAVAAKEGLKAVIE
                     EATDLTASVPAWLQADHDNLTGKVLRMPDRVDIDIPVQEQLIVELYSK"
     gene            32833..33777
                     /locus_tag="VFD74_06225"
     CDS             32833..33777
                     /locus_tag="VFD74_06225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZK49178.1"
                     /translation="MLDFHSPKITMEKVSDNRGVFVVEPLDRGFGYTFGNSLRRVLLS
                     SLDGAAASAVKIEGVAHEFSTISGVKEDVTDIILNLKKAVFVLHGEDGEIDVELVKDG
                     PGVVTVGDIAATANLEIVNPGAILATLGEGARLEMVIWVRRGRGYVSAGENKQEETIL
                     GVIPIDSIFSPVSRVAYRVEAARVGQRTDYDKLILEIETNGAVDPRQCLEEAANLLIE
                     QLRIFADEDRIIVEDQEPIFFGLQEPAGSGLEDMLIEELELGVRSYNCLKREGVETVS
                     DLISRTEAELLNIPNFGKKSIEEVRERLAERDLKLRGE"
     gene            33779..34129
                     /gene="rplQ"
                     /locus_tag="VFD74_06230"
     CDS             33779..34129
                     /gene="rplQ"
                     /locus_tag="VFD74_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZK49179.1"
                     /translation="MRHQRKGRRFGIQPSHRRQMLANLACSVLEHGRVKTTEAKAKEV
                     KPLVEKAITMGKAGDLHSRRTAISMLRNKKISYALFNEIAPKYAERQGGYTRILKLGP
                     RLGDAAEMVYLELV"
     gene            34148..>34855
                     /locus_tag="VFD74_06235"
     CDS             34148..>34855
                     /locus_tag="VFD74_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK49180.1"
                     /translation="MGVAQEMDSAGASAAPALCFQQVSFRYRADRPLAICDFDLEVAP
                     GEILAVVGGNGSGKSTLARLCNGLLVPERGAVLVGGVSTADPAHSWTVRSRVGLLFQN
                     PEDQIVGASVEDDVAFGLENLGTPRADMRVRVSEALEAVGLAGEELTEPHLLSGGQKQ
                     RLALAGVLVLEPTVLVLDEPTSMLDPTGRAEVMAAVRRLTARGVAVVLITQHMDEAVV
                     GDRLVALDAGRAGYLGSP"
BASE COUNT         6962 a        10678 c        11451 g         5764 t
ORIGIN      
        1 ggtgccccac cccggtcgat cccgaccacc ttcggagcag aagccccttc cctcctcacc
       61 ggatgcctga gtcgtactgg atggagacct cggtgaaagc gtgcggctcg accgctacgt
      121 cgtagggatc ggcgcggggc aacggtccgg gactctgcgg caccagcgtg tatcggccgg
      181 gagcgaggtc gacgctgaac cgaccgtcct cagaactccg gacctccgcg actgtcgcac
      241 cggagctgtt cgtgacggtg atggtcgctt cgtagggggc ttcaccagac tcgccctgca
      301 tttgcaccgg tgaggtcggg cctatggtga ccacgccccg cacgccgctg tccgttggag
      361 gggccccgaa cacgcggtaa ggctagcgga gacggcgagg gccaacagga tcatacgagt
      421 gcgcacttct cggcctcctt ggcgtcggtt agcccggttg ctcgaccgac gtgcggtctc
      481 tgccctcttc cttgctccgg tatagcaggc ggtcgacccg ctgcaagatg gattcgggat
      541 cgtcctcgag gttcgcgagc gtggctcctg cggagacggt cactgagatg ggggaggggg
      601 tgtccgtgag gctggaggtt ctgacgaggg cacggaatct atcggccacg gccgtgaggc
      661 tttcgaggtc gatgttggtg accaccgcca agaactcctc tcctccccag cgccccacca
      721 ggtcgaaggg ccgggtcgcg agctccagtg tcttggccac catcgtgagg acgcggtccc
      781 cggtggggtg gccgaacacg tcattcacgt tcttgaaatg gtccacatcc aggagcaccg
      841 ccccgaacgg ccagccatag cgctgcagtt ctccaaggcg ctgttccagc tgtgattgga
      901 ggtagcggcg gttcggcagg gccgtcaagg cgtcaaggta ggcgagttcc tcgagctcgg
      961 cgatgcgtcg ggctgcttct ggggcatctc gatcgggcgc gaagatctcc gccgctccca
     1021 cgatcgttcc ggcctggtcg cgcaggggca gcgagcgcac ccgcacgggt accctattgt
     1081 ccgtcggcgt ggtgcaggaa cacgtgggct tcacgcggat ccccatccgt catggtcgcc
     1141 tcgagagggc agccacccat gcagagctgg gttccctctt catcgacgtg catcagcagg
     1201 ttgtcacggc acctgcttcc gactacccgg gatgcgggga acccggtgat ggcctcagct
     1261 cctcgattcc agtagaggac gcgacggtcg cggtcggtga agtagacgcc ctcgtcgaga
     1321 tcgtcgagca aggagcccat caagcgtggg tcgcgcggga tcttccccac gtcctcttcc
     1381 gtggatccaa caggtccgtt cggcatcgct tcccctccgt cgcatgctgt ccgccagcat
     1441 cctgcagtca tcagtgtacc tgggtcggcc cgttcgggtc cgcacacggg ttgacgcctc
     1501 cgatcctgcg gcgtatgcgg actgggccga aatggccgat tgccgcttcg gtgtggccgc
     1561 gccggtgccc ctcttctcct tgaaatggcc tatctcaacg ggtatcatcc ggagtcgccc
     1621 caggggtcat cgaccgtggg ggggtgcccg agtggccaaa gggagcagac tgtaaatctg
     1681 tcggcgtagc ctacggaggt tcaaatcctc ccccccccac cacccacacc accggggcgc
     1741 agcgcgcacc atgtacgcga cgtaagggcc tccatagctc agctggcaga gcacttccat
     1801 ggtaaggaag gggtcatcgg ttcgaatccg attggaggct ccatagtgca ttctggatcg
     1861 tggcggcgta gctcagttgg tcagagcagc ggaatcataa tccgcgtgtc ggaggttcga
     1921 gtcctcccgc cgctactagg agcccgtttc agaatggcgg ctcgccgcct tcgtcttgga
     1981 gcgggctctc ggtttggacc caagatgcgg cccccggcgg ggccgcatct tgggtttcaa
     2041 tatgcgctgt ggtagcatgc acgattgctc tgaagctagg atagggtgaa gcgatgcgcg
     2101 tacgagtaac gctggcgtgc caagagtgca aacgccgcaa ctacactacg atgaagaaca
     2161 aacggaacga tcccgagcgg ctcgaattcc agaagttctg caagtggtgc ggttcgcaca
     2221 cctcgcacaa ggagacgaag taagggacag ggtcgccctc catggggtga atccgggctc
     2281 ccatccacgg atgagcgcag ggcagtagct ccaacggtag agcgccggtc tccaaaaccg
     2341 gatgttgggg gttcgaatcc ctcctgccct gccagtctct caaggaaagg catacgaagt
     2401 acgatggcca gaacgaccac cacaaagact tcggcgaagg ttccggccaa ggccgtggcc
     2461 gcaccgaaga ccaccaagcc ggttgccggc gccgctcctt cgcggcgcgg ccggcccacg
     2521 cgtttcctca gggaagtgcg caccgagatg agcaaggtga cgtggccccc tcggcaggaa
     2581 ctcgtgcagg ccaccatggt cgtcatcgtc gcggtggcca tcgccgcttc ctacatcggg
     2641 gtattcgacc tcatctggtc ttccctggtc aacctggccc ggctcggcta gtccacggcc
     2701 gagtagcgaa aggacccacc cgcttatgct ggccaagaag tggtatgtga tcaatacgta
     2761 ctcggggcac gagaacaagg cgcgagccaa cctcgagcac cgtatccagt ctatggacct
     2821 cgcaccgtat ttcgaggaca tcgtcgtgcc caccgaagtg gtggtcgaga ccaagggcgg
     2881 cgagaaggtg cagacagaga aacgcatctt ccccggctac gtcctcgtga agatgaacct
     2941 caccgacgac tcgtggtccc tggtcaagaa cactccgggc gtcactgggt tcgtcggttc
     3001 agagaacaag cccacggcgc tgagcccggc ggaggtcgac cgcatcctcc acacctccac
     3061 cgccgagaag ccgaaggcga aggctgagtt ctcggtgggc gaccaggtga aggtcaccag
     3121 cggtcctctg gccgagttca ccggcgaggt ggccgaggtg aacatcgagc agagcaagct
     3181 caaggtcatg gtgtccatct tcggacgcga tacgcctacc gagctcggtt tcgaacaggt
     3241 caagcggatc tgacgcgcgc ggatcccagc gaagcacacg gagagagata gtggcaaaga
     3301 aggttaaggc aaatatcaag ctgcagatcc cggcgggcca ggccaatccg gcgcctccgg
     3361 tgggtcccgc cctgggccaa cacggcgtca acatcatgga gttctgcaag gcctacaacg
     3421 cccagacaca ggacaagatc ggcaccatca tcccggtgga gatcacggtc tacgaggaca
     3481 ggtcgtatag cttcatcctc aagaccccgc cggcggccgt gttgatccgg caggcggcag
     3541 gcgtgcccaa gggctccgga gtgccgaacc gtgacaaggt gggccgcatc acccgcgagc
     3601 aactccggca gatcgccgaa gtcaagctcc ctgatctcaa tgcgaacgac gtcgaggcag
     3661 ccatgaacat cctggcgggc acggcgcgca gcatgggcgt gaccgtagag aagaagtagg
     3721 tagcatcgat cgggtgtcgc gctccgcggg cagatcgcga gtgcggcagc cgacgtctgt
     3781 gatatcggcg cccggctccg ccgggcgcca cgaagtggga gggccgcgag gcccgcatgc
     3841 accacaggag gaacgaacat gagcaagggt tccaagcgct tcgaagaagc caagcgcgcc
     3901 gtcgaaaccg gcagggtcta tctccccggc gacgccatcc gtctcatcaa atcgctcgag
     3961 acggccaact tcgacgagac cgtcgaggtc cacatcaagc tcggcgtcaa tccgcgccac
     4021 gccgaacagc aggtccgtgg caccatcatg ctccccaacg gcacgggcaa gaccatccgc
     4081 gtggccgtgt tcgccaaagg cgacaaggct gccgaggccg aagcggccgg cgccgatgtg
     4141 gttggcgcgg aagatctagc cgcacgggta gagcagggct tcctcgactt cgacgcggcc
     4201 atagccactc ccgacatgat gggggtcgtg ggcaaattgg gtcgtgtgct cggcccccgc
     4261 ggcatgatgc cgaacccgaa agccggcact gtgaccttcg atgtcggcaa ggccgtgcag
     4321 gatatgaagg ccggcaagat cgagtaccgc accgacaagt tcggcgtggt gcacgtgatc
     4381 atcggtaaga agtccttcgg cgagcgcgaa ctcatcgaga actacgctga gttcatcgac
     4441 gagttggtgc gggtcaagcc gtccgcggcg aagggcaagt atctgcggtc gatcaccttc
     4501 gccagcacga tgggtcccgg tgtccctgtg gacacgacca agacccgcgg gctgctggaa
     4561 gacgaagcca cggcctagca gcggggcgat tggccggcgg ggcgacggcg ccgcggcgcc
     4621 ggggttgtag gcgcaaggta gcagggctat actgtcaagc ctgaagccgg agacagctgg
     4681 tgccgcccga aggggtggtc caatcggtcc gaagggatcg gccagccgag gcgagatgcg
     4741 accgagttat gaaccgcgcg tgaagttgcg cgagacgact gtggcgtcca cctcccttcc
     4801 ggaggtggac gtttcttttg cgaaaggagg gaagacatat ggcaaagccg gagaaagtag
     4861 cggcggtaga tcggatcatc gagaagttgc gctctaccga tgtgtactac ctggtcgatt
     4921 tccgcggttt gtctgtgggc gacgccaccg atctgcgcag tcgcctgcgg gccaacggag
     4981 ccgacctcat agtcgtgaag aacacgctgg ccaaacgcgc cgcggctgag gcgggcatcg
     5041 aggggctcga cgggctgttc gagggcccta cggccatcgc gtattgtggc gacgatcccg
     5101 tggcgcccgc gaagacgctg gtgggtttcg tcaaggagaa gaagaagctc gtcatcaagg
     5161 gcgggttcct gcagagtcag atcctgctgc aggcacaggt ggagcaactc gcccagttgc
     5221 cgagccgcga agagctcatc gcaaaggtcg ttggcggtat cgccgcgccg ttgtatgggt
     5281 tggcgaacgt gctcaccggg cccatccgcg gcctcgtggt agcgctcgac cagatcaggg
     5341 agcagaaagc cgcggcgtag cccggctcgt cgtgagacca gggcgggcag atcccgccgc
     5401 gtatctgaac gtatggaggt agaacgagca tgaccccgca agaactgatc gaagagatca
     5461 agaagatgac ggtcctcgag ttggccgaag tcgtcaaggc gctggagacc gagttcggcg
     5521 tgaccgccgc ggctccgatg gccgccgccg ccgcccccgc ggccgcagtc gaagaagtcg
     5581 aagagcagac cgaattcgac gtgatcctct ccgctgtcgg cgagaagaag atccaggtga
     5641 tcaaggtggt ccgtgccctc accggtctcg gcctgaagga agccaaggac ctggtcgatg
     5701 gtgctccgaa tcccgtgaag cagggcgtca ccaaagccga ggcggaagaa ctgaagaagc
     5761 agctcgaaga agccggagct gccgtcgaga tcaagtagcg cacagcctcg ttcgctcagc
     5821 gcaggctcgg cgggtacgac tagcttcgtg cctgccgcgc ttgtggctgc ctgttccctt
     5881 atgatagcat ctgagtttgc gcttgcagtc cggcgcgcac tcagagacca cgccgacctt
     5941 tctgcgagga gtgagcaagc ttgagcctca gcacgactcc caagccgcgt aagtcattcg
     6001 ccaaacttat gcccatcctc gatgtccccg agctgatcgc tatccagctc gagtccttct
     6061 cgtggttcct caaggacggt ctgcgggaga ccattcggga catcaatcct atcgaggact
     6121 acacgcagaa gtatgcggtg gagttcgggg agtacgagtt ccgcgatcca cctctgtcgc
     6181 tggaggagtg ccgcaacaag gacatgacct acgcggctcc tctcttcatc accgtggcct
     6241 tcgtcaatcg tgagaccggt gagatccgtg agcagcaggt cttcatgggc gatttcccca
     6301 tgatgaccga ccagggcaca ttcatcatca acggcaccga gcgcgtcatc gtgacccagc
     6361 tggtgcggtc gcccggagcc tacgtgcagc tgcccaagga cagcaacaag gcgacgcagg
     6421 tgttggtcgc gaacctcatg ccgtcccgag gctcgtggct cgagttcgag atcgacaagc
     6481 gcggcacggt caacgtgcgt atcgaccgca agcgcaagct cctcgccacg gtcttcctgc
     6541 gggcgctcgc cggcatcaag aacagcgaga cgggcgacaa gcccgggatc ctcgagaccg
     6601 gcagcgacga ggagatcctc gccgccttcg gtgacaacat gttcatccgc aacaccctcg
     6661 agaaagacac ggtgcgctcc gtcgaggagg cgctggtcga gctgttcaag aagcagcgtc
     6721 cgggcgaacc gccgacgctg gaagccagcc gcactctcct gcgcaacacc tgcttcgacc
     6781 ccaagcgcta cgacctgtcg agggtggggc gccacaaggt gaacacgcgc ctcgggttgg
     6841 acatagagga catccgcacg ctgacgaaag aggacatcat cgctctcgtc aaagagctgg
     6901 tggtcgtgcc tctggaggcg tcgaagaagg cccctgaagg cgcgatcgcg ttctgggacg
     6961 agtcccgcga tctggccgaa gcggctacga acctcgaatg ggacgacgtc gcccaccgcc
     7021 tcgacgagta cgaggacttc ggcaaccgcc gcctgcgcac tgtgggcgag ttggtgcagg
     7081 aagcgttccg catcggcctt tcgcgcatgg agcgcgtggt caaggaacgc atgaccaccg
     7141 aggacgtcga caccatcacg ccgcagacca tcatcaatat tcggccggtg gtggccgcgc
     7201 tcaaggagtt ctttgggagt tcgcagctct ctcagttcat ggaccagacg aactcgctgg
     7261 cggggcttac ccaccggcgt cgtctgtcgg cgcttggcgc cggtggcctc acccgggaac
     7321 gtgcacccat cgaagtgcgc gacgtgcacc ccacacacta cgggcgcatg tgccccatcg
     7381 agaccccgga aggaccgaac atcggcctca tcggttcgct ggccgcctac gccaagataa
     7441 acgagttcgg cttcatccag accccctatc ggcgcgtggt cgagggcaag gtcaccgacg
     7501 atatcgtgtg gctctcggcc tatgacgagc gtcagtgcac catcgctcag gccagtgcgc
     7561 ccttcgatcc gaagaccggc gagttcctca acgacaccgt catggcccgc tcacctggtg
     7621 gcgagtacgt cagcgccctc gccggccagg tggagttcat ggacgtctcg ccgcagcaga
     7681 tcgtgtcggt ggcgaccgcg ctgatcccgt tcttggagca caatgacgcg aaccgggccc
     7741 tgatggggtc caacatgcag cgtcaagcgg tgcccctgct cgtgtccgac gccccgcttg
     7801 tgggcaccgg gatggagtat cgcgccgcgg tggacacggg cgacgtcgtg cgcgctcgtc
     7861 gccccggccg cgtcaagggc gtctcggctg ttcgtatcct tgtcgaatcc gagtctggtg
     7921 agatagacaa gtactcgctt ctcaagttca cgcgctccaa ccagggcacg ctgatccatc
     7981 agaagccgct ggtcgacgtg ggcgacgacg tggtcgaagg cgcagtgctg gccgacggcg
     8041 cgtccaccag cctgggagaa ctcgccctgg gccgcaacct tctcgtggcc ttcatgagtt
     8101 gggaaggcta caacttcgaa gactccatca tcatcagcga gcgcttggtc atggacgacg
     8161 tgctcacctc gctccacatc gaggagcacg aggtggaggc gcggtcgacg aagctgggcg
     8221 atgaggagat cacccgcgac atccccaatc gctccgagga ctccctgatg gacctcgatg
     8281 ccgaaggtat cgtgcgcatc ggtgctgaag tcagctcggg cgacctgctg gtgggcaaga
     8341 tcacgcccaa gggcgagacc gaactcacgg ccgaagagaa gctgatccgg gccatcttca
     8401 aggagaaggc tcgcgaggtg cgcgacacgt cgctcaaggt gccccacggc gaacgcggtg
     8461 tggtcatcga cgtgaagcag ttctcgcgtg agaacggcga cgacctctcg ccgggcgtga
     8521 accgcctggt gcgcgtgtac gtggctaaga agcgcaagat cgccgagggt gacaagctcg
     8581 ccggccgcca cggcaacaag ggcgtcatct ccaagatcgt ccccgaagag gacatgcctt
     8641 tcatggagga cggttctccg gtggacatca tcctgaaccc cttgggtgtg ccttcgcgca
     8701 tgaacgtggg gcaggtcctc gagacccatc tcggttgggc cgcgagtcag ggcttctaca
     8761 ccatcaagcg caataacgtg tcgttgagcg acgccaacgg gagcagcgac ctgggctggc
     8821 ggggacagct ggatctggtg cgcaagtact acgatgtggt gtccaagtcc accggcgaag
     8881 gtcccatgcc catcgcctcc cccgcattcg acggtgccac ccaggtggat gtcgacgagg
     8941 ctctccttgg gtgggccctt gcgcaggacg accggagacc ggctcccacc gacggcgtgg
     9001 agccttgtcg cgtggagatg cgggcggatc tctcacgtca cgccggcacc caggtgagcg
     9061 gcaagatcaa gctcttcaac ggccgcacgg gcgagcccta cgaccagagc ataaccgtgg
     9121 gctacatgta tatgttgaag cttcaccatc tcgtggacga caagattcac gcgcgttcca
     9181 ccggcccgta cagcttggtc acccagcagc cgctgggagg caaggcacag ttcggcggac
     9241 agcgtttcgg cgagatggag gtgtgggcgc tcgaagcgta cggcgccgcc tacaccctgc
     9301 aagagatgct caccatcaag tcggatgaca ccgtgggtag ggtcaaggca tacgaggcga
     9361 tcgtcaaggg cgagaacatc gctcgcccag ggatccccga gtcgttcaag gtgctgctga
     9421 aggagatgca gtccctgggc ctcgacgtga gcgtcatcag tgaagagggc ggccatctcg
     9481 aggtatcaga cgaggaggac gacctgctgc gggcggccga ggagttgggc atcgacctga
     9541 cggctcggcg tcgcgccgaa gagcgtgcgg ctgaggccca ggcggccttg gtcagctcga
     9601 acccaggcga cgactacgac gacgctttcg atgacgatga tgatgacggt gacgaggacg
     9661 ccttcgaaat cgacgagagc gatatcgcgg ccgcgttggc cgttgacctc gccggcatcg
     9721 gcggtaccga agacgaggag ctgtcccgtc ccgtagtcga cgacgaggaa taggagcccc
     9781 ccttgatcga catcaacgaa ttcgacgcca tcaagatcgg cctggcgtct ccggagcaga
     9841 tccgggcgtg gtcgtcaggt gaggtcacca agccggaaac gatcaactac cgcacgctga
     9901 agcccgagaa ggatgggctc ttctgcgagc gcatcttcgg tcccaccaag gactgggagt
     9961 gctactgcgg caagtacaag cgcgtccgct acaagggcat catctgcgaa cgctgcggcg
    10021 tcgaggtcac ccgggcaaag gtgcggcgtg agcgcatggg tcacatcgac ttggccgccc
    10081 ccgtctcgca tatctggtat ttcaagggtg tgcccagccg catgggctac ctgctcgata
    10141 tctcgcccaa gcgtctcgag cgcgtgctct acttctcagc ctccatcgtc acgcacatcg
    10201 acgaggccaa gcgcgaggcc gacaaggaga agctcgagga gctgatccag gccgatatcg
    10261 acaacctttt cacggctcgc gacgaacgca aggctcggat cctggccgag cgcgacaagc
    10321 gcgtgagcgt gatcatgggc aactccaccc ccgacgagga cgaccaagcg gccctcggcc
    10381 tcgacgaggt gcccatcgcg gagtggacca agaaggagat aaccaagcgt tctgccgagt
    10441 tgcgcgagca cgccgacctg gccctgcgtg aactagagcg ctacgccgag gagcaggccg
    10501 aacgcacgcg cgagatgtgg gccgcgttcc gcaacctgaa gcagggcgag atcatcacgg
    10561 atgagacctt cttccgcgat ctcaaagaga agttcggaaa cccgttcggc tacggcatct
    10621 atttcaaggg cggcatgggc gcggaggcca tccgtgagct cctgcgggcc gtggacctcg
    10681 agaccgaggc cacgtcgctg cgcgagatca tcaagacgag caagggccaa cgtcagagtc
    10741 gtgccgtcaa gcgcctcaag gtggtcgagg gcttcctgcg gagcgacaac cggccggagt
    10801 ggatggtact cgaagccatc ccggtgatcc cgcccgagtt gcgtccgatg gtgcagctcg
    10861 acggcggccg tttcgctacc agcgacctca acgacttgta ccgccgcgtc atcaaccgga
    10921 acaaccgtct caagcgcctg ctcgacctgg gcgctcccga gatcatcgtc aacaacgaga
    10981 agcgcatgct gcaggaagcc gtggacgcgc tcttcgacaa cggccgtcgc ggtcgtgccg
    11041 tcacgggccc gggcaaccgg ccgctcaagt ctctgtccga catgctcaaa ggcaagcagg
    11101 gacgcttccg tcagaacctt ctcggcaagc gcgtggacta ctcgggtcgt tcggtcatcg
    11161 tggccggtcc ggagctcaag ctccaccagg caggtatccc caagaccatg gcgctcgagc
    11221 tcttcaagcc gttcgtgatg agccgcttgg tggaacgccg tcaggtgcag aacatcaagg
    11281 ccgccaagaa gatcgtggag agcgagagcc ccgaggtgtg ggatatcctc gaggaggtca
    11341 tcaccgagca tccgatcctt ctcaaccggg cccccacgct ccaccgtctg ggaatccagg
    11401 ctttcgagcc ggtgctgatc gagggcaagg ccatccagct gcaccccctg gtttgcaccg
    11461 ccttcaacgc cgacttcgac ggcgaccaga tggccgtgca cctgcccttg tcggtggagg
    11521 cccagtcgga ggcgcgcata ctcatgctct cctccaacaa catcctctcc ccggcccacg
    11581 gtaggccgat cgccgtgcct tcgcaggaca tgatcctggg cgtctactac atgacctact
    11641 acgagcctct cgaggggcag cccgagttgg aggcggtaga cgtcgccgat cctgaagcgc
    11701 tgcacgcgtt ggatccgcag ccccgcgtct tctccgaagt ggccgaactt gaacacgcct
    11761 acctggacca caacgtcggt cttcaggacc ccatactcct tcgctgggac ggtcagcgca
    11821 tcatgaccac ggtcggccgc gcgatgttca actccgccgt ggtgacctcc ctggccggat
    11881 acttcggcga cgcctgggcc ggcggccccg acggcggccc cgggcgcaag gacgactacg
    11941 aataccgcaa ccgggtcttc acgaagaagg tgatgaccga ttttatctcc gacttggtgg
    12001 agaccttcgg agccaccgcg gtggctcacc tgctcgacgt cctcaaggac ctcgggttcc
    12061 gctacgctac cgtcgccggt gtgacgatct ccaagaacga catcgtcgtg ccgcccgaga
    12121 aggagcagat cctccagggc tacgaggaga aggtcgcacg catccagaag gagtacatgc
    12181 gtggcaatct caccgaggtg gaacgccggg agcgggtgac cgacctctgg accgaagcca
    12241 ccgacaaggt cgccgaggcc atggaggaga acttccgccg gctgaacccg gtgtacatga
    12301 tggccaactc gggagcgcgc ggctccttca agcagatccg ccagctcgcg ggtatgcggg
    12361 gtctcatggc caacccgaag ggcgagatca tcgagcgacc catcaaggcc aacttcatgg
    12421 aaggcctctc ggtcctcgag tacttcatct ctacgcacgg cgcccgcaag ggtctggccg
    12481 acaccgcgct gcgcaccgcc gactcgggct acctcacccg tcgtctggtc gacgtggccc
    12541 aggatgtggt catccgcgag gtggattgcg gcacgaccga atccatcgtg ctgccctacc
    12601 ggcgcgacga taagtcggaa catctgctcg gccgtctctt cggcgagaac atcgtcgacc
    12661 cggagacggg cgaggtgctc caccaacgca acgacaccat caccaagcgc gcgctgcgcg
    12721 atctcgacaa ggtcttcccg gctgacgagc acaaagaaat gaagctcaag gtgcggtcgg
    12781 tgctggtgtg cgagagcgag ttcggcgtgt gccaacgctg ctacgggcgc tccctagcca
    12841 ccaatgaact ggtggccatc ggtgaggccg tgggacacgt ggccgctcag tccatcggcg
    12901 agccgggcac acagctcacc atgcgcacct tccacaccgg tggtgtggcg ggcgcagaca
    12961 tcacgcacgg cctcccgcgc gtggtggagc tcttcgaggc ccgcaagccg aagggccagg
    13021 cgcgcatcgt cgacgcggag ctggcgcccg agcgcgagta cttcgtcgaa ttcgacgatc
    13081 ccgcgaaccc gcggcgcatc cagatcgtcg acgacctcgg cgaggaggcg gcgtcctacc
    13141 cggtgagccg acgcatgcag ctcatggtga accacggtga ccgcgtctac gcgggcgatc
    13201 agctctacca agggccgctc aatccgcacg atgtgctgca gcaccgtggc atccttggta
    13261 cgggtcagta cctggtgagt gaggtgcaga aggtctacaa gagccagggc gtggacatca
    13321 gcgacaagca catcgagctg attgcgcgac agatgaccaa acgcgtgaga gtggacgcct
    13381 gtggcgacac gcacttcttg cccgggcaga ctgtcgacaa gatcgccttc ctgcgtgaga
    13441 acgaagcggc cgtggccgtc ggcgaagaac cggccacgtt cgaggtcatg atcatgggca
    13501 tcacgaaggc ctccctggcc acggagagct tcctctcggc ggcctccttc caggagacca
    13561 ccaaggtgct caccgatgcg gccctcgaag gcaagaccga ccacctacgc ggcctcaagg
    13621 agaacgtgat catcgggaag ttgatcccgg cggccaccgg tctccgcagg tatcgcaaca
    13681 tcgaggtcaa cccggtgcgc accatccggc ccgacgtgga aggcttcttc ggggcgggtg
    13741 ccggccagac cgacgtgggc cgcagtggca cgctgaccac cgagcaggca ctggccatgc
    13801 tgggcggcgc aggtgacggg tgggccgacg cttcggtcga cgacctcgat ctgcccacct
    13861 acgtgcactc gatcctcacc ggccacggca tcgaggcgct cgccgcgctc ctcgagagga
    13921 cgccggaaga gcttctgggt atctccggct tcgggcagaa gagcctggag gaagtgcgga
    13981 accgcctggc cgaccgcggc ctggcgctga agggcgacga ggtcgagggc gtggatcagt
    14041 cgctcaccga cgacgaccag gacgacttcg agatgtccgt gtccgaagat acacaggagg
    14101 actgatgact ccttgtaggc cccgtgaccc tttgctatac ttcgccgccg cggctgagtc
    14161 cgggttggtg atgagtcacg cccttcggtc acagcggtat ctgacaagca gtaattcgaa
    14221 tgtgactgag gagccttcgt gccgaccata aatcagttga tacgcaaggg ccgcgccgag
    14281 ctggccaaga agagcaagac gccggcgctc aaaggtgctc cgcagaaacg gggcgtctgc
    14341 acccgtgtgt ataccaccac acctaagaag ccgaactcgg ccctgcggaa ggtggctcgt
    14401 gtccgtttga ccaacgggat ggaggtcacc gcctacatcc ctggtatagg acacaatctt
    14461 caggagcact ccgtggtgct catcagaggc ggccgggtca aagacctccc gggcgtccgg
    14521 tacaaggtca tccgtgcggc cctcgatacc gctggtgtgg cgaatcgcaa gcagagccgc
    14581 tccaagtatg gtgtcaaggc gcccaagtag agcgggaagg tggctgtaga tggctcgtag
    14641 gataaaggcg cagaagcgcg agatccagcc cgatccggtg taccggaacc ggctggtgac
    14701 ccagatcacc aaccgtctca tgatcgacgg caagaagtcg accgccgaac acatcgtgta
    14761 cggcgctctc gacatcatgc gcgaacgcac ggggcaagac cccatcgcgg tgctgaagga
    14821 agccgttgag aacgtgcgcc ctgccctgga agtgaagtcc cgccgcgtgg gcggcgcgaa
    14881 ctaccaggtg cccgtcgagg tggctacgcg gcgttcgtac acgctggcgg tacggtggtt
    14941 catcacgcac agccgcgttc gtcgtgagaa gagcatgtcc gagcgtctcg ccggcgaact
    15001 gctcgatggg ttcaaccgca ccggcgtcac catgaagaag aaggaagacc tcctgcgcat
    15061 ggccgaagcc aacaaggctt tcgcccacta ccgttggtag cggcgcggcg ggcagtcgga
    15121 cataggtagt ctcaggcagt tgagagtgta ggatccgggt ctgtggggcc gggacggccc
    15181 cacgattcgg ttgtgttcgt ggtatcgccg ggccggacgg tccgcccggc gggtgaaggg
    15241 atagagagaa gaagtggcac aggcaacgac atcgaaaacc gacacgggga agcgtctgtt
    15301 cccccttgag cgcgtgcgca atatcggcat catggcccac atcgatgccg gcaagaccac
    15361 gaccaccgag cgcatcctct actacaccgg tcgcacctac aagatcggcg aggtgcacga
    15421 cggtgctgcg gtgatggact ggatgcagca ggagcaggag cggggcatca ccatcacggc
    15481 tgcggccacc acctgcgagt ggaagcggca tcgagtgaat attatagaca cacccgggca
    15541 cgtggacttc actatggagg tcgaacgctc ccttcgtgtg cttgacggtg cggtggccgt
    15601 attcgacgcg gtagccggtg tcgagccgca atccgagact gtttggcgtc aggccgacaa
    15661 gtaccgggta ccccgcatcg ccttcatcaa caagatggac cgcacgggcg ccgatttcgg
    15721 gcaggccgtg cagaccatga tcgacagact ccatgcccgg ccgatggttg tgcaactccc
    15781 cgtgggggcc gaggacggct ttcgcggcat catcgatctc gtgaggatgc gcgcctacct
    15841 cttccaggac gacatgggag tcgagtggga agatgctgag atacccgcgg acctggtcga
    15901 tgaagccgac atagctcggg cgcacatgat cgagatcctc gccgactacg acgagcagat
    15961 catgcacgcc tatctcgagg gcgacgaggt ctcggaggcg gcgttgttgg cggccatccg
    16021 cacgtccacc ctccagatcg gcgcgacacc ggttctgtgc ggttcggctt tcaagaacaa
    16081 gggcgtccag atgctgctcg acggcgtgat cgactacttg ccgtctccgc tggacatccc
    16141 gccgatggtc ggtttcgatc cgcgcaccga cgccgagatc accaggctcg cctccgacga
    16201 tgagccgttc agcgcgcttg ccttcaagat catgaccgac cctttcgtgg ggaagctgac
    16261 cttcttccgc gtctactcgg gtgttctcaa ggctggatcc tacgtctaca acgcttccaa
    16321 aggtaagaag gagcgcgtgg gccgcatcct ccagatgcac gcgaaccacc gcgaagaccg
    16381 cagcgagatc ttcgccgggg acatcgcggc ggccgtgggg ctcaaagcca ccacgaccgg
    16441 cgacaccctc accgatccgg attatccggt gatcctggag tccatcgagt tccctgagcc
    16501 cgtgatctcg gtggccatcg agcccaagac caagttggat caggaccggc tgggcgagag
    16561 cctcatgaag ctggccgagg aggacccgac cttccgggtg cacaacgacg aggagaccgg
    16621 gcagaccatc atcgccggca tgggcgaact ccacctggag atcatcgtgg accgcctgct
    16681 gcgtgagttc aaagtggacg ccaacgtggg tcgtccgcag gtggcctacc gtgagaccat
    16741 acgcgctgtc gtcgagaagg ccgaaggccg gttcgtccgc cagacgggtg gtcgcggcca
    16801 atacggtcac gtgctgctcc gcatgtcgcc ggccgaacag ggtgcaggtt tcaccttcac
    16861 caacaaggtc gttgggggct cggtaccccg cgagtacgtc gctccgactc aggccgggat
    16921 cgtggaggcg atgagtggcg gtgtgctggc aggcttcccc atggtcgaca tcgacgtaga
    16981 gctctacgac ggctcctacc acgatgtcga cagttccgag atggccttca agatcgccgg
    17041 ttcgatggcg ttcaaggctg gagctgagaa gggcaagccg gtgcttctcg aaccaatcga
    17101 agacgtggag gtggtcgttc ccgaggaata catgggcgat gtcatcggag acctgaactc
    17161 ccggaggggc cacatcttgg gtatggagcc gcgtgccggc gcccaagtgg tgcgcgcaca
    17221 agtgccgctc tcagagatgt tcggatacgc caccgacctg cggtcttcca cccagggtcg
    17281 cgcgacctac accatgcagt tcatccacta cgcggaagtg cccgcgtcca tagccacgga
    17341 gatcgtcgcc cgattccgcg gcgacaaata acacaagaca aaaaggcaat acggccgggt
    17401 caccggtctg agcgcatcac ggtaagaaaa gggaggaaag atggccaaga agaaattcga
    17461 acggacgaag ccgcacgtta acgtcggtac tatcggtcac gtggaccatg gcaagacgac
    17521 cttgacggcc gccatcacgg cctgtctagc ggcgcgcaat ccgaatgtca ccgtccggtc
    17581 gttcgactcc atcgacaatg ctcccgagga gcgcgagcgc ggtatcacca tcgccacggc
    17641 gcacgtggag tacgagaccg cgaaccgcca ctacgcgcac gtcgattgcc cgggtcacgc
    17701 cgactacatc aagaacatga tcaccggtgc ggctcagatg gacggcgcca tcctcgtggt
    17761 ttccgccgcc gacggtccca tgccccagac ccgtgagcac atcctgctcg cccgtcaggt
    17821 tggtgtgccg tacatcatcg tcttcctgaa caaggccgac atggtggacg atcccgagct
    17881 cttggagttg gtcgagatgg aagtgcggga gctcctcacc gagtacgagt tccccggcga
    17941 cgacatcccc gtgatcaccg gctcggcact gaaggccctc gagtgcgcct gcggcggcga
    18001 cgagtgcgag gcctgcaagc cgatcctcga gctcatgaag gcggtcgacg agtacctgcc
    18061 cgagcccgag cgcgacaccg agaagccgta cctgatgccg atcgaggatg tgttcaccat
    18121 ccaaggtcgc ggcaccgtag tgaccggtcg tatcgagcgc ggcatcgtca aggtgggcga
    18181 cgacgtgcag atcgtcggca tgcagaagga ggtcgtcaag accgtctgca ccggagtgga
    18241 gatgttccgc aagctgctcg acgaaggccg ggcgggcgac aacgtgggcg tcctgctgcg
    18301 tggtatcaag cgtgacgacg tgcagcgagg catggtcctg gccaagccgg gcagcatcac
    18361 cccgcacacc gagttccgcg ctgaggtgta tgtgctcacc cgcgaggaag gtggccgtca
    18421 caccccgttc ttcaacggct accgcccgca gttctacttc cgcaccaccg acgtcacggg
    18481 tgactccacc ctcgacgagg gcgtggaaat gatcatgccc ggcgacaaca cgaacctgac
    18541 cgtcaaactc atcaccccca tcgccatgga ggaaggcctc cgcttcgcta tccgcgaagg
    18601 cggtcgcacc gtcggcgctg gcgtcgtgac gaaagtaatc caataggcac cgacgttcac
    18661 gatcactgcg gggtcccgga ctggggcccc gtgggatcga acagcgagag gcgaggctga
    18721 ccttggctca acagaagata cgcatacgat tgaaggccta cgatcacgag ctcatcgaca
    18781 agagcgcgcg tagcatcgtg gagacggcgg agcgcaccgg ggcgacgata tcgggtcccg
    18841 tgccgcttcc cacggagaag aacgtgtatt gcgtgatccg cggtcccttc aaggacaagg
    18901 attctcgcga gcacttcgag atccgcacgc acaagcggct gatcgacatc caccagccta
    18961 ctccgaagac ggtggattcg ctcatgcgca tggacctgcc cgcgggcgtg gacatcgaga
    19021 tcaaactgtg atgaaagcac tgcttggaac caagataggg atgacccagt tgttcggcga
    19081 ggatggcgtc gtcacgcgtg tgaccgtgat ccaagccggc ccctgcgtag tcacccagaa
    19141 gaaggacgtc gcctcggacg gctatgccgc cactcagatc gcgttcggcg acgtgaaagt
    19201 caagaagctc acgaggccgg aactcggtca cctgaagaag tcgggggcct cccctaagaa
    19261 gcacctggcc gaagtgcgcg gggagcacga tctcgcggtc ggcgaccgtg tgaccgtgga
    19321 ggcgttcgca cccggtgaca tggtgagggt caccggcgtc agcaagggca agggtttcca
    19381 aggcgtgatc aagcgtcacg gcttcaaggg tgggcctgcc agccacggct cccgcttcca
    19441 tcgggccccg ggctccatcg gcgccagcgc cgacccggcc cgtgtatatc cgggcacgaa
    19501 actgccgggt cagatgggca accagcgtgt gacccagacc ggtctcacgg tcgtcgccgc
    19561 cgaccccgag cgcaaccttc tcatggtcaa gggctccgtc ccggggagca aaggctccct
    19621 ggtgttcgtc agaggttagg gatatgccaa cgatacgaaa aatcgacgag aagggcgccg
    19681 tcatcggcga gcacgagttc gcggacagcc gtgtggagac cgagatgaac atcggtctgt
    19741 tgcatgaggt cgtccggggc gaacaggccg cccgccgtca gggcacgcac gccgccaaga
    19801 cgcgcggcca ggtgtccggt acgggagcca aggcgttccg ccagaagggc accgggcgcg
    19861 cccgggccgg cagcaccaga gtgtcgcagt ggaccggcgg cggcttcgcg ttcccgcccg
    19921 tgtcgcgcga ccactccttc aaagtgaaca agaaggtgcg cgtcaaggcg ttccgcatgg
    19981 ccttgggcaa tctgatcgcc aatgacgcgg tgcgggtctt gactggagtc gagttcgaag
    20041 tgccgtcgac caagcgggct tcggccatct tggattcggc cgcgctcgat ggtcccacgt
    20101 tggtggtggc cgcctcgcaa gagatgaaca cgttgctgag cttccgcaac ctcccgcgca
    20161 cccgcgtcct cccggtgaac gaggtcgacg tgcaggacta cctctgggcg cgaagcgccg
    20221 tgctcacaga agccgccatg gcgatcctgg aaggcggtgc gaactgatgg acgcccggca
    20281 agtgatcatc aggccggtca tctcagagaa gagctacgag gcggtggaga agggccgcta
    20341 ctccttcgag gtacatacca aggccactaa acagcacatc cgtgacgctg tggaggagat
    20401 cttcaaggtc tccgtcatcg gtgtgaacac gatgaacgtg aagcccaaac cgaagcgtcg
    20461 cggagccttc aagggcacga cgcgccggtg gaagaaagct gtcgtcgaac tggctccggg
    20521 caaccggatc gagttcttcg gcggcaacta agaggctagg agagcggaag aggctcatgg
    20581 cgatcaagag ttacaaacca acgtcacccg gacgtcgctt cacgaccact ccggccttcg
    20641 atgagatcac gaagaccgag ccggagaagt cgctgatcgt cccggtgaag cgcaagggcg
    20701 gccgaaacaa caacggccgt atcaccacgc gtcaccaggg cggcggccac aagcggatgt
    20761 atcggatcat cgatttcaag cgcgacaagg acggggtccc cggccgcgtc tcgtcgatcg
    20821 agtacgatcc caaccgcagt tgccgcatcg ccctgatcgt gtacgccgac ggcgaaaaac
    20881 gctatatctt ggcgccgaac aagctgaagg tgggcgacca ggtagaggcc ggcccgggtg
    20941 ccgacatcaa ggtgggcaat gcgttgccct tgaagaacat cccggtgggc tccacggtgc
    21001 acaacatcga gctgcacatc gggaagggcg gccagatcgc ccgcgccgcc ggcacatccg
    21061 cccaggtcat ggccaaggaa gggaactacg ccacgctgcg tctgccctcc ggcgagatgc
    21121 gcatgatcca cttggcctgc cgggccacag tcggcgagat cggcaactcc gagcacgaga
    21181 acctctccac cggcaaagcc ggccggactc ggtggatggg catcaggccc accgtgcgcg
    21241 gcacgaccat gaaccccgtg gaccatccgc acggcggcgg tgaggggtct acgacgcccg
    21301 gccgtcatcc ggtgactccg tggggtgtac cgactctcgg tcataagacc aggggcacaa
    21361 aagcgtctga caagtacatc atcagacgac gcggtaaagg gaggtagaag gcgctgtgac
    21421 gagatcgagc aagaaagcgc ctttcgtcgc ggagaagctc atgctccgta tagagcgcat
    21481 gaacgccgcg ggcgagaagc ggatgttgaa gacgtggtcg cgtgcttcca cgatctaccc
    21541 cgagatggtg ggtcacacta tcgccgttca cgatgggcgt aagcacgtgc ccatctacgt
    21601 gacggagagc atggtgggcc acaagctggg agagttcgcc ctcacgcgca cctaccgggg
    21661 tcatggcggc ggcaaagaga agcggtcgag gaggtaggcc gtggtcagcg ctacagccaa
    21721 gtatgtgcgt ctttcgcctc gcaaagcgcg tcaggtcgtc gacctgatcc gcggcaagtc
    21781 tgtgcgtgac gcgaaggcca tcctcatgtt gacgcctcgt ggtgcggctc ccatcgtgca
    21841 gaaggtcctc gcttcggccg tggccaacgc ggagaacaac aacgacatgt cggccgacga
    21901 cctctacgtg gcgaatgcct acgtgaacga aggccccacg ctgaagcggt tcaagccgcg
    21961 ggcgatgggt cgggcgacgc cgatccgcaa gcggaccagc cacatcaccg tggccgttga
    22021 cgagagaagg gagtcgtaac ccgtgggaca gaaggttcac ccgggtggca tgcgcctcgg
    22081 catcatccat gattggaagg cgcactggtt caccgagaag aatttcgcgg acttcctcca
    22141 agaggacctc gcgatccgga agtacatccg gcgtcgcatg ggccacgcgg gcctgtccga
    22201 gatagtcatc cgcaaggaca ccaacaaggt gatcatcgac atccataccg cacgtccggg
    22261 catcgtcatc ggcaagtccg gtgccgaggt ggatggcctg aggcgtgaca tccacgggat
    22321 gacgggcaag aacgtgcacg tcaacatcgt cgagatcaag cgtcccgagt tggacgccgg
    22381 cttggtggcg cagtcgatcg cggagcagct cgccaaccgg gtcagtttcc ggcgcgcgat
    22441 gaagcgtgcg atcacctcgg ccatgcggtc cggcgcggcc ggtatcaagg tcaggtcggg
    22501 cgggcgtctg ggcggtgccg agatggcgcg cgtcgagggc tacagcgagg ggcgagtccc
    22561 gctgcacact ctgcgcgccg acatcgacta cggcttctgg gaagccaaca cgaccttcgg
    22621 caagatcggg gtcaaggtgt ggatcaacaa gggcgagatc atgcccgagg gtttcgacag
    22681 cgacgctcgc aaagagccgg cgctcggagt gcccgaagaa cgacgtggcg gccgcagtgg
    22741 ccgtgggcgc cgaggcgaag gttccaggtg gtagtcccca tcagcaaccc ggtgcgccga
    22801 cgcgttagcg cgtcggcaac gtctgggacc gtggacaggg actgaccagc ctcagggaga
    22861 caaggaacga tgctactacc gaagaggata aaacatagaa agcagcaacg tggccggatg
    22921 ggcggcaagt ccaagggcca gacgactgtg cagttcggcg agttcggcct ccaggctctt
    22981 gagccgggtt gggtcacggc caggcagatc gagtccgctc gtattgccat gacccgcaag
    23041 atgaagcggg gcggcaaggt gtggatcaac atcttcccgc acaagccgat caccaagaag
    23101 cccgccgaga cgcgcatggg ttcgggcaag ggttcgcccg accaatgggt ggtcgtggtg
    23161 aagcccggcc gcgtcatgtt cgagttggcc ggggtgccgg aggacttggc ccgcgaggcg
    23221 atccgccttg ccgtgcacaa gcttcccctc aagtgccggt tcgtggtgag ggaggacggc
    23281 agctttgaag gctaaagaca taatcgagct cgccgagcac gagttggagc aacgtctcaa
    23341 ggaatcgcgg gaggagctgt tcaacctgcg cttccagcac gcgaccggcc agcttgagaa
    23401 caccggtcgg ctgcgccagg tgcgccagga catcgcgcgc atcctgtctg ttcagaactc
    23461 acagaagtcg aaggagtcgt agatgaccgc ggagcgtgag agttcgcagc gcgagcgccg
    23521 aggcgtcgtg atctccgaca agatggagaa gaccatcgtc gtagaggtgg cggtagccaa
    23581 gccgcacccg ctgtacaaga agatcgtgcg tcgcagtagt cgcctcaaag cgcacgatga
    23641 gaacaacgag gcgcggatcg gggacgtcgt cctggtacgt gagtgccggc cgctctccaa
    23701 gtcgaagagt tggcgcctta tcagcattct ggagcgggcc gagtagcccc tcgaccgaga
    23761 gagataggta actagcgatg atccaagtag agacgagact caaggtggcc gataataccg
    23821 gcgctcgcga gctcctctgc atccgcatca ggggtgggtc gcaccgtcgg tacgccggcg
    23881 tgggcgacat catcgttggc acggtgaagg aggccactcc gggcggctcg gtgaagaagg
    23941 gcgatgtggt gcaggccgtg gtggtgcgtg tgaagaaatc ctacgggcgc aaagacggca
    24001 cgtacatcgg cttcgacgag aacgccgcgg tcctcatcga ccgtcagaag aacccccgcg
    24061 gcacgcggat attcggcccc gtcgcccggg agttgcgcga gaagaacttc gcgaagatcg
    24121 tgtccttggc tccggaggtg ctgtgatgaa gaagcaggct cccagagtca ggaagatacg
    24181 gaagggtgac accgtcgagg tgatccaggg caaggagcgg ggaaagcgcg gcaaagtgct
    24241 tcgcgtgctt gccgaagacc agcgcgtggt cgtcgagcgg atcaacttca tcaagcgcca
    24301 cgtccggccg tcgcagaaga atccgcaagg cggagtcatc gagcgcgaag ccgggatcca
    24361 cgtgtcgaac gtgaagctgg tatgcccgag ctgcgacgcc gcggtgcgcg tgggaatgcg
    24421 cgaggagcgt ggcgacaagg tgcgatactg caagaagtgc aacgtccagg tagataaggc
    24481 ttagcgatgg ctattccgag attcagagaa aagtacacga gccaggtggt gcctgagctg
    24541 ctgtccgagt tcggctacag caacatcatg caggtgccac gtattcagaa gatcaccgtc
    24601 aacatgggtg tgggcgaagc tgctcaggac gccaagctac tcgaggaggc ggccaagcag
    24661 ctcgggcaga tcaccgggca gaaagcccag gtgcgcagat cccgcaagtc catagcgaac
    24721 ttcaagatcc gtgagggcat gccggtgggt ctgcgcgtga ccctgcgggg cgaccgtatg
    24781 tgggagatgc tcgaccgact catcagcatc gccctccccc gcatccgcga cttccgtggt
    24841 atctccccga aagggttcga cgggcagggc aacttcaacc tcggtctcaa ggaacagatc
    24901 atcttccctg agatcgacta cgacaagatc gatcgggtcc gtggtctgga tgtcgccatc
    24961 accaccaccg cggaaactga cgatgaagct cgggcgctgc tcaagaagtt gggcatgccg
    25021 ttccgcgaga gctagagaag gagcagcagg gtatggcaaa gaagtcgctc atcgcgaagg
    25081 cgaaccggac gccgaagttc tcttcgcgtg cttacaaccg ctgcagacgc tgtggccgcc
    25141 cccgcggata tttccgccgg ttcggcctct gccgcatctg cctgcgtgag ctcgcgcacg
    25201 agggagtaat ccccggcgtg accaagtcga gctggtaggg aggaggacga tgtcacaatc
    25261 agatcccgtc gccgacatgc tcacgaggct ccgcaatgcc aacaaggcgc tgcactcaga
    25321 ggtctcgatg cccacgtcgc acctgaaggt cgaagtggcc cgcatcctga aggacgaggg
    25381 ctacatcgcc ggctacaaag tggtcaaggg cgagagctac gacacgcttt tggtcgacct
    25441 gaagtacacc gaggaccgcc gccgggtcat cacccagctc aagcggatca gcaagcccgg
    25501 tcgtcgtatc tatgccaaga aggacaagct gcccaaggtc cttggcggct tggggatggc
    25561 tgtcgtctcg acttctcgcg gcgtgatgag cgctcgcgag gcccagcgtc tgggtgtcgg
    25621 cggcgaagtc atctgcttcg tgtggtgatg atgcatgtct aggatcggaa gagaacccat
    25681 caacgtgcct gcgggcgtcg aggtcaagat cgacgggcag cacgtgacag tcaagggccc
    25741 caagggtctg ctgagccgcg acctgaatcc cgcggtggac gtgcaactcg ccgacggcgt
    25801 gatctcggtc acccggccga gcgactcggc cactcacagg tcgctccacg gactgacccg
    25861 taccttggtg gcgaacatgg tcatcggcac caccacgggt ttcgagaagc gtctcaacat
    25921 cgtcggcgtg ggataccgcg ctgcgatgaa gggcaccgat ctcgaggtcc aggcgggata
    25981 ctcccaccca gtggtcgtga atccgccgga caacatcgaa ttcgaggtgc cggtgcccac
    26041 gctgatcgtg gtgaagggca tcgacaaaca gcaggtcggc caggtagccg ccaacatccg
    26101 ggccatcagg aagccggagc cgtacaaagg caagggcatc cgctatcagg gcgaagtcgt
    26161 ccgccgcaag gtcggcaagc gggcctaggg gaggatgaac agagggacat gagcagaatc
    26221 gccaagatca aacgagccag tgaccgtcgc gcaaagcgcg tgcggggagg ggtgcggggc
    26281 acgaccgaac ggccgcgtct caccgtcttc cgctcgaacc gcgggatctg ggctcaggta
    26341 atcgacgaca tgaacggcac gactctcgcg agtgctggaa gcatcaacat cgcggagaaa
    26401 ctgcccaaga gggagatggc cgccaaggtc ggggagcttc tcgctgaacg agcgaaggcg
    26461 gccggtgtgg cccgcgtcgt gttcgaccgc gggccctacc tgtaccacgg ccgagtcaag
    26521 gcccttgccg acggcgcccg cgagggcggc ctcgagttct agaaagggag aggtacggca
    26581 gtggcaacga ttaaagctga agcgctgaat cttcaagaga acgtgattga gatcaaccgg
    26641 gtggccaaag tcgtgaaggg tggtcgacgg ttctcgttca ccgccctcgt ggcggtcggc
    26701 gacggagaga gcatcgtggg tgtgggctac gggaaggcca aagaagtccc gttggccatc
    26761 cagaaggcca tcgacgacgc gaagaagaac ctgttcgtgg tgccgaagca cggctccacg
    26821 atcacgcacg gcgtcacggg ccggttcggt gccgggcgcg tgctgttgcg gcccgcttcc
    26881 cccggcaccg gagtcatcgc cggcggcggt gtgcgtgcgg tgctggagct gggaggcgtc
    26941 cacgacgtgc tcaccaagtg cctgggcacc acgaatccta tcaacatggt gcgtgcggtg
    27001 gagcaaggat tgcgttcgct gcgccgtcct gaggagatcg ccgcgctgcg gggcaagacc
    27061 gtgcgcgagg tcctgggcat ggagaaacgg gagcatccgg catccaccga gatcgcggga
    27121 gagtgaccga cgtgcttgcc atcacgcaga ctcgaagcgg catccgccgc aagcacgacc
    27181 acaaggctac cctgcgggct ctcggcatct cccgcatggg gcagactgtg taccacgacg
    27241 ataacccggt gatccgaggc atggtgaaga agatcgagta catgctcgaa gttgccgagg
    27301 tcgatgtgaa ggacgagggg taggtcatgg gagaaatctt cctgcacaac ctgcgcccca
    27361 cggagggctc gcgcagcaag aagaagcggg tcggccgcgg agaggggtcg ggcaaaggca
    27421 agacctcagg caaaggtcac aagggcgcga acgcccgcag cggcggggga gtctccgccc
    27481 gctacgaggg cggtcagatg ccgctgcaca tgcggctgcc caaactgcgt ggtcccctgg
    27541 ccaagacttc tatggccatc ggtccgttcc gcacattcat gactccggtc aacatctctc
    27601 gtctggcggc tttcccggcc ggcgcagagg tcaccccgga ggcgctcgtc gagatgggca
    27661 tcatcaagaa ggccgacgag agggtcaaga tcctcgccga cggtgagctc gaccgcgccc
    27721 tcacggtgcg cgcgcacggg ttcagcgcgg ctgccaagac caagatcgaa gctgccggcg
    27781 gcacggccga ggtcctctag ggaccgcggc ctcgccgctg caagatagac gggagagagc
    27841 tagatgttcc aatccctgct gaatgcgtgg aggcttcccg acctgcggaa gcgcctcgtg
    27901 ttcacggcgt tcatcatcgc aatgtatcga ctgggctcgt tcatcccggt cccgggtgtc
    27961 gatttacagg cgatcaagga tctggtctcc ggaggcgggg tcttcgcctt cctcgacctg
    28021 ttcgccggcg gcgccctgga acgtgtcgcc gtcttcgccc tcgggatcat gccctacatc
    28081 acggcgtcga tcatcatgca gctgctccag atcgtcatcc cgcagcttga agcgctggca
    28141 aaagaagggg aatcgggggc caagaagatc aatcagtaca cccggtactt caccgtggtg
    28201 ttggcgctca tccaatcgac cggctacgtg atcattttca agtcccaagg ggcgttcacg
    28261 accgcgaacc ctctgaaccc cctgaggttc taccttatcg tgatgactct gaccgtgggt
    28321 acggccctag tgatgtggct cggtgagctg atcacccagc gcggcatcgg aaacggtatc
    28381 tcgctcatca tcttcgcgag tatcgtctcg cgcctgccca gcggtctcta caagatgttc
    28441 tccacgatga accccctgga gtggctggcc atggctctca tcgctctggg cgtcatcgcg
    28501 gccatcgtgc tcatacaaga ggggcagcgt cgcatcccgg tgcagtatgc caagcgggtc
    28561 gtcggccgca agatgtacgg ggggcagagc acatacctgc cgctgcgtgt caacatggcc
    28621 ggcgtcatcc cagtcatctt cgcctcggcc atcctcgtgt tccccgtgac cttggctcag
    28681 atgacgccgt ggggttgggc gcagtccgcg gcccgcgccg tagggccggg caccggtatg
    28741 tatctggtcg cagaggtcct gctcatcatc atcttcacct acttctacac ggcggtgcag
    28801 ttcaacccgc tcgatcaagc cgacaacctg aagaagtacg gcggcttcat cccggggatc
    28861 cgtcccggta ggccgaccgc gcagtatctc gacaaagtgc tgacccgcat cacgctcccc
    28921 ggcgcggtct tccttgcctc catcgcggcg cttcccacca tcttgatctg ggtgatgggt
    28981 ataccgttct tcttcggtgg cacctccctg ctgatcgtgg tgggtgttgc gctcgacaca
    29041 atgaagcaga tggagtcaca gcttctgatg cgacactacg aaggcttctt gaagtaagca
    29101 caagggggag cgaggatggc gcagttcgat ctgatcctgt tgggaccgcc cggggccggc
    29161 aagggcacgc aggccgggcg gatcgtcgag gactactcga tcccgcacat ctccaccgga
    29221 gacatgttgc gggccgccgt caagaatcag acggcgatgg gtctcgaagc caagcgctac
    29281 atggacgcgg gcgacctggt gcccgactcc gtggtcatcg gcatcgtcaa ggatcgcctg
    29341 caggagcccg acacggctgg cggcttcctc ctggacgggt tcccgcgcac gatcccgcag
    29401 gcggaggcgc tcgacgacat gctcgagagc atcgagcgcg gagtctccaa ggctctggcc
    29461 atcctcgtgg atgaggaggc actggtccgc cgactcagcg gccggcgcat ctgtcgcgat
    29521 tgtcagaccc ccttccatat ggagttcggt ccgccctccg tcgcaggcgt atgtgacaag
    29581 tgcggagggg agctctacca gcgcgacgac gacagcgaag cgaccgtgcg caaccgcctg
    29641 gccgtctacg gtcgccagac ggagccgttg atcgcctact acgactcgca gtccgtcttg
    29701 gcgcgcatcg acggcgacca gactccggac gaggtgtacg aggatctcaa agaggcaatc
    29761 ggtcgccctt gacgctcgca acgtgatcat caggaagaca cccgaggaga tcgaagcgat
    29821 cgcacgggca gggcgcatcc tcaggggttg tctggacgtg ttgagtcaga aggtggagcc
    29881 gggagtgtcg accaaggagc tcgatcggtt ggccgaggag ttcatccgcg gtcacggcgg
    29941 cacccccacc ttcaagggtt accgcggatt ccccggctcg atctgcacat cgcccaacga
    30001 catggtggtg cacggcatac ccggctccac caggcttcgt gagggcgaca tcgtaggcat
    30061 cgacgtggga gtgacgctcg atgacttcat cgccgacgcg gcggtcaccc tggcagtggg
    30121 cgacgtctcc gaagaagctc aggccctcat gaccgcgact cgggagtcgc tcatgttggc
    30181 cgtggaggag tgttggccgg ggaagcggat cggggacatc tctcacgccg tgcaatccca
    30241 cgtcgaggtc cgtggctact cggtggtgcg cagtcttgtg ggacacgggg tgggcagggc
    30301 catgcacgag gatccgcagg tgcccaactt cgggcttccc gggcgaggtc cgcggctgtc
    30361 cgagggcatg gtgttggcga tcgagcccat gatcaacagc ggtgggttcg acgtcgaggt
    30421 cggtgccgat tcgtggtcga tattcacgaa agacgggggc ctttcggctc acttcgaaca
    30481 cactgtcgcc gtcacggcgg ccggtccgcg ggtgttgacg gcctagcgat ccgccgatga
    30541 gggccgtctg gcgacgcccc gggtgtcgct cgcggggtcg acatcgacgg tttcgcggtc
    30601 gtgatgcagg gcgtgtgtta atataggcgg tcggtacgcg gccgaaccgg gttcggccgg
    30661 gatctcgaga gaagaggtgt accagaagca gtgcccaaag aagacgcgat agaactcgag
    30721 ggtgaggtag tcgaagccct ccccaatacc atgttccggg tcaagctcga gaacgaccac
    30781 gaagtgctgg cccacatctc gggcaagatg cgcatgaact acatccgcat actgccgggc
    30841 gacaaggtca aggtggagtt gtctccctac gacctcactc gcgggaggat cacctaccgc
    30901 ttcaagtagt gtgaggtggt gtggccgtcg acatgacggt cgatcggaat cgcccccgtc
    30961 ttggtcaacg gatgtggtgg cgtggtagtt ggaagcaatc ggtcccgata gcccgggacc
    31021 gtcgccggct ctgagacatc agcccggggc ttcgtgcccg gggcggcggc tgcagtacga
    31081 tgtcgagggg cctgatagag gtgctcagtc atgtgttggt acgactttgc cgcggatgcg
    31141 gcgagtccgg ccgagagggt tgagacgaag atgaaggtac gagcgtcggt aaagccgatg
    31201 tgtgagaagt gtaaagtgat ccgccgcaag ggcgtggtcc tggtgatctg ccagaacccg
    31261 cgtcacaagc agcggcaagg ctgatcggga gaagagtatg gctcgaatcg ctggagttac
    31321 tctccctcgg gaaaaacgag tcgagatcgg gctcacctac gtgtacggga tcgggcgttc
    31381 caggtcgaac gtggtcctgg cggccgtggg cgtcgatccc aacacctacg tgaaggatct
    31441 caccgaggac gaggagatcc gtctacgggc gtacatcgag gagaacgtga tcgtcgaggg
    31501 cgaccttcgc cgtgagcgca gtcagaacat caagcggctc atggagatcg gctgctaccg
    31561 gggtatccgc catcgtcgcg gactgcccgt gcgtggccaa cgcaccaaga cgaacgcgcg
    31621 tgtgcgcaag ggtcacaagc ccaccgtggg tgttaagaag aaggttcgtt gatcggtgag
    31681 catccgctga ccgcgatcga aacgatcggg agatagccac atggcaaaac cagtaaagca
    31741 tggcggaggg cgtgtgcggc gccgcaaggt gcgcaagaac atcgccgtcg gcaacgcgta
    31801 tatcaagaca tcgttcaaca acacgatggt cacgcttacc gataaggaag gcaatgtgat
    31861 ctcgtgggcg agcgccggcg tggccgggtt caagggttcg cgcaagtcga ctcccttcgc
    31921 cgcccaggtg actgctgaaa aagcagctcg cgccgggatg gagcacggcc tgcagaaggt
    31981 cgacgtgttc gtgaagggcc cgggtagcgg tcgcgagacc gccatccgtt cacttcaggc
    32041 cgcgggcctc gaggtaggcg gcgtaaagga ctgcaccccc atcccccaca acggatgccg
    32101 ccccaagaag cggcgccgag tctagcgcgg agagagaagg agtatggcaa gggatacctc
    32161 agcacagtgc aagcagtgcc gccgcgaggg gatcaagctt ctcctcaagg gtgagcgctg
    32221 tctcacggac aagtgcgcaa tcgataggcg cggctacccg ccgggcgaac acggccgcgg
    32281 gcgtaggcgc aagcagtccg agtatcttgt gcagctgcgc gagaagcaga aggcacgcag
    32341 gtattacgga gtgctcgaga agcagttcca caactactat gaggctgcac accgccagac
    32401 cggcgtcacg ggcgagaacc tgctgcgcct actggagttg cgcctggaca acgtcgtgta
    32461 ccgcctcggt ttcggcgcgt cccgccggca ggcgaggcag ctgatccttc acggacattt
    32521 cctcgtgaac ggcaagcgtg tgaacatccc gtcgtacaag gtgcgaccgg acgagatcat
    32581 tacggccgtt gcggcgaagg aaggcctgaa ggcggtcatc gaagaagcga ccgatctgac
    32641 cgcttccgtg cccgcctggc tccaggccga ccacgacaac ctgacgggta aggtgctgcg
    32701 catgcccgat agggtcgata tcgacatccc ggtgcaggag cagctcatcg tcgagctgta
    32761 ctcgaagtag gggcggcaag gtattgacca cggcaggcgg atgaaccggc agccgtggag
    32821 taggaggact gattgctaga cttccattcc ccaaagatca cgatggaaaa ggtgagcgat
    32881 aaccgcggcg tgttcgtcgt ggaaccgctg gaccggggat tcggctacac ttttggcaac
    32941 tccctgcgtc gggtgctgct ctcttcgctc gacggagcgg cggcttcggc agtcaagatc
    33001 gaaggagtcg cccacgagtt ctccaccatt tcgggggtca aggaggatgt caccgacatc
    33061 atcctcaacc tgaagaaggc tgtcttcgtg ctgcacggcg aagacggtga gatcgacgtg
    33121 gagctggtga aggacgggcc gggcgtggtc accgtcggcg acatcgccgc aacggccaat
    33181 ctcgagatcg tcaacccagg cgcgatcctc gcgacgttgg gcgaaggcgc ccggctggag
    33241 atggtcatct gggtgcgccg tggccggggc tacgtgtcgg ccggcgagaa caagcaggaa
    33301 gagacgatcc ttggggtcat ccccatcgac tcgatcttct cgcctgtctc gcgagtggca
    33361 tacagagtcg aggccgcccg tgtcggacag cgcaccgact acgacaagct gatccttgag
    33421 atagaaacga acggcgccgt cgatccgcgc cagtgtctgg aagaggccgc caacctgctc
    33481 atagagcagc tccggatttt cgccgatgaa gaccgtatca tcgtcgaaga ccaagagccg
    33541 atcttcttcg gtttgcagga gcccgcgggc agcggtctgg aagacatgct gatcgaagag
    33601 ctcgagctgg gcgttcgttc ctacaattgc ctcaagcgcg aaggtgtgga gacggtctcc
    33661 gacctgatct cgcgcaccga ggccgaactg ttgaacatcc ccaacttcgg caagaagagc
    33721 atcgaagagg tgcgtgaacg tcttgccgag cgggatctga agctcagagg agaatagcgt
    33781 gaggcatcag agaaaaggga ggaggttcgg catccagccg agccaccgcc ggcagatgct
    33841 ggccaacctc gcctgctcgg tgctcgagca cggacgtgtg aagaccaccg aggcgaaagc
    33901 caaagaggtc aagcccctgg tggagaaggc gatcaccatg ggcaaggccg gtgatctgca
    33961 ctcccggcga acggccatca gcatgttgcg gaacaagaag atctcgtacg cactgttcaa
    34021 cgagatcgct cccaagtacg cggagcgtca gggcggctat acccgcatct tgaagctggg
    34081 ccctcggctc ggtgacgcgg cggagatggt ctacttggag ctcgtctaga ggaacgacgg
    34141 gatacttgtg ggcgtagccc aagagatgga cagcgccggg gcctcagcgg ccccggcgct
    34201 gtgctttcag caggtctcct tccgctatcg ggccgaccgt ccgttggcga tctgtgactt
    34261 cgacctggaa gtcgctccgg gggagatcct tgccgtggtg ggcggcaacg ggtcggggaa
    34321 gagcactctg gctcggctct gcaacggcct actggtgccc gaacggggtg ccgtactcgt
    34381 gggaggggtc tcgaccgccg accctgccca ttcttggact gtgcgctccc gcgtgggtct
    34441 gctgttccag aatccggaag accagatcgt cggcgccagc gtcgaggatg atgtcgcctt
    34501 cggcctggag aacctgggga cgcctcgtgc ggacatgcgc gtccgcgtga gcgaagcgct
    34561 cgaggctgtg gggctggcgg gcgaggagct gaccgagccc cacctcttgt cgggggggca
    34621 gaagcaacgg ttggccttgg ccggagtctt ggtgctcgag ccgacggtgc ttgtgctcga
    34681 cgaacccacc tccatgctcg acccgaccgg ccgggcggaa gtgatggccg cggtgcggcg
    34741 cctcacggcg cggggggtgg ctgtcgtgct catcacccag cacatggacg aggcggtcgt
    34801 cggtgaccgc ctggtggctc tcgacgccgg acgtgcgggc tacctcgggt cgccc
//
LOCUS       DASRMJ010000219         2937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_306013, whole genome shotgun sequence.
ACCESSION   DASRMJ010000219 DASRMJ010000000
VERSION     DASRMJ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306013"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFD74_06240"
     CDS             complement(<1..546)
                     /locus_tag="VFD74_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK49181.1"
                     /translation="MNLPAQFRTAWRSAQHTFRALRIRNYKLFWFGQFASLTGTWAQG
                     VAQAWLVLKLTNSPLVLGTVVTVRFAPTLVFSLFGGVIADRLPKRRLLFITQSVLLLQ
                     ALAVGILTWTGAVLLIHLYIAAVVRGLADSVDRPTRQAFVVELVGPEELANAVPLNST
                     QFNAARIIGPAIGGAIVATLGI"
     gene            complement(543..986)
                     /locus_tag="VFD74_06245"
     CDS             complement(543..986)
                     /locus_tag="VFD74_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK49182.1"
                     /translation="MDTASLQRFRSAYFDVVHELDTIRLRAWEHSRITLPQLRVLFQI
                     RRTPGITVGRLSKLLGVTFSTTSGLVSKLSERGLVARSTVPGDRRQATLDLTEAGMAQ
                     AAAVSGDALAYLVAVGEALGDRLPGVVDSLEELARVARELREARG"
     gene            complement(1108..1926)
                     /locus_tag="VFD74_06250"
     CDS             complement(1108..1926)
                     /locus_tag="VFD74_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZK49183.1"
                     /translation="MERFLFRLGRSKHASRFVLKGGLMLLAWRAPVSRPTKDIDMLAS
                     LDNDVQVVEDVVRDVCLVAVEPDGMTFDPVSVSGRVIVEQAEYPGVRIRLLALLGTAR
                     VTLQLDVGFGDLVVPGPTGLDYPSLLADFPAPALVGYSRESVIAEKFHAMVRLGRLNS
                     RMNDFFDLWMLPRQFDFDGQLLADAVSTTFAHRHTDVPTEPAVFETAFPTYPGKAEQW
                     RSYLKRNSIEGAPSGFADVTTYIRTFLGPITTALVRESSFERRWKAPGPWRRST"
     gene            complement(2031..2639)
                     /locus_tag="VFD74_06255"
     CDS             complement(2031..2639)
                     /locus_tag="VFD74_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HZK49184.1"
                     /translation="MNEQSSIERALEVFRRAGGILRTHQVLAAGVHPRTLYAMRDTGL
                     LDRLGRGLYRLADMPPLGNPDLATVAATVPHGVVCLVSALSFHELTTQIPHEVYLALR
                     RGVEPPRLEHPPLRLFWFTGAMFTEGIERHRVDGLTLQVYSPAKTVADCFRYRNKLGL
                     DVALEALRLYREGSSFAVEDILYYANICRVETVIRPYLEALV"
     gene            complement(2741..>2937)
                     /locus_tag="VFD74_06260"
     CDS             complement(2741..>2937)
                     /locus_tag="VFD74_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49185.1"
                     /translation="ARRKRKEGDSPGAEAGGQPAPVSVSASDLATYFLDVAPARTAQA
                     LGDRVGAALYVEPYVVRGDS"
BASE COUNT          563 a          923 c          986 g          465 t
ORIGIN      
        1 gatgcccaag gtggcgacta tcgccccgcc tatcgccggg ccgatgatgc gggccgcgtt
       61 gaactgggtg gagttgagcg gaacggcgtt ggccaactcc tcgggcccca ccaactcgac
      121 cacgaaggct tggcgcgtcg gtcggtcgac ggagtcggca agccccctga ccaccgccgc
      181 gatgtagaga tgtatgagca ggaccgcacc cgtccacgtc agaatgccca cggcgagggc
      241 ctggagcagc aggacgctct gagtgatgaa gagcaggcgg cgtttgggga gacggtcggc
      301 gatcacgccg ccgaagaggg agaagacgag agtgggagcg aagcggacgg tcaccaccgt
      361 ccccagcacg agcggtgagt tcgtcagctt gagcaccagc cacgcctgag ccaccccttg
      421 cgcccacgtg ccggtgaggg aggcgaactg tccgaaccag aacagcttgt agttgcggat
      481 tcggagcgca cggaaggtgt gctgcgcgga acgccacgct gtgcggaatt gggccggcag
      541 gttcaccctc gggcctcccg gagttcgcgg gccacgcgcg cgagctcctc caacgagtcc
      601 accactccag ggagacgatc gcccagcgcc tccccgacgg cgaccaggta cgccagggca
      661 tcgcccgaga ccgcagccgc ttgagccatg cccgcctcgg tcagatccag ggtagcctgt
      721 cgccggtcgc ccggcacggt cgagcgagcc accagtccgc gctccgagag cttggatacc
      781 agcccgctgg tagtcgagaa ggtcacaccc agtagcttgg agagccgccc caccgtgatc
      841 cccggcgtcc gccggatctg gaagagcacc cgcagctggg gcagggtgat gcgcgagtgt
      901 tcccacgcgc gcaggcggat cgtgtccagt tcgtgcacaa cgtcgaagta cgcggatcgg
      961 aaacgctgga gagatgcagt gtccacattc ctatgatacg gcaacaccga actatgtggt
     1021 ggtaccgtag caattcaaag gcgcagcacg gaagcggcga agctcagcag cgcgcccttg
     1081 ccaatcgcac ggattgattc gacggcgtca ggtcgacctg cgccaaggcc ccggggcttt
     1141 ccaccgtcgc tcaaacgaac tctcccgaac cagcgcggtc gtgatcggac cgaggaacgt
     1201 acggatgtac gtggtcacgt cggcgaagcc gctcggtgca ccctcgatgc tgtttctctt
     1261 gagatagctg cgccattgct ccgccttccc agggtaggtc ggaaaagccg tctcgaaaac
     1321 tgccggctcg gtcggaacat cggtgtgccg atgtgcgaag gtcgtcgaca cggcgtccgc
     1381 aaggagctgt ccgtcgaagt cgaactgccg cggcagcatc cacagatcaa agaagtcgtt
     1441 catgcggctg ttcaggcgac ccagtctcac catggcgtgg aacttctcgg cgatcacgct
     1501 ctcccggctg tagccgacga gggcgggagc agggaagtcg gcgaggagag aaggatagtc
     1561 aaggccggtc ggaccgggga cgacgaggtc gccaaatccc acgtcgagct gcagcgtcac
     1621 gcgggcggtt cccaagagtg cgagcaggcg gatccgcacc cccgggtact ctgcctgctc
     1681 cacgatcacc ctcccgctga cgctcacagg gtcgaaggtc atcccgtcag gctcgaccgc
     1741 caccaggcac acgtctctca ccacgtcctc tactacctgg acatcgttgt ccagcgaggc
     1801 cagcatgtcg atgtctttgg tggggcgcga gactggcgcc cgccaggcaa ggagcatgag
     1861 gccgcctttg agcacgaaac gcgacgcgtg cttggacctt cccaaacgga agaggaagcg
     1921 ctccatagcg aagtattgca ggagctcatt gaaggggcga tggctctctc gggccagcga
     1981 aagcaagcgg tcgtgcacgg aggccgggag gtttcggggc cggtccatcc ctaaacgagg
     2041 gcttcgaggt aaggccggat gaccgtctcc acccggcaga tgttcgcgta gtagaggata
     2101 tcctccacgg cgaaggagct gccctctcgg tagaggcgaa gggcctcaag agcgacatcc
     2161 agaccgagct tgttgcggta acggaagcag tcggccaccg ttttcgccgg actgtagacc
     2221 tggagggtga gcccatccac tcggtggcgc tcgatgccct cagtgaacat ggcccccgtg
     2281 aaccagaaga gccgaagcgg cgggtgttcg agacgggggg gctcgacccc tcgcctaagc
     2341 gccaggtaga cctcgtgggg gatttgagtt gtaagctcat ggaaactaag ggcggagacg
     2401 aggcacacga cgccgtgggg caccgtggct gcgaccgtcg ccagatcggg attccccagg
     2461 ggaggcatat ccgcgagccg gtaaaggccg cgcccgagcc ggtcaaggag tccggtgtcg
     2521 cgcatggcgt agagggtgcg aggatgaacg cctgcggcca ggacctggtg tgtccgtagg
     2581 atgccgcccg cccttcggaa tacctcgagc gcgcgctcga tgctggactg ctcgttcatg
     2641 atgtggataa agataccagc aattgcgtcc gtctgctggt aaatctatcc gcggtagccg
     2701 gtgcccactc tttgatcacg ttgttgcggg caaaccgacg tcagctgtct ccgcggacca
     2761 cgtagggctc gacatagagt gccgccccca cccggtcccc gagagcctgt gccgtccgcg
     2821 cgggggccac gtcgaggaag taggtggcga ggtcggaggc tgagacggac acaggggctg
     2881 gttgccctcc ggcttctgct cccggagaat cgccctcctt ccgcttccgg cgcgccc
//
LOCUS       DASRMJ010000220         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_137203, whole genome shotgun sequence.
ACCESSION   DASRMJ010000220 DASRMJ010000000
VERSION     DASRMJ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137203"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..632
                     /locus_tag="VFD74_06265"
     CDS             138..632
                     /locus_tag="VFD74_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZK49186.1"
                     /translation="MIVLSHTGIPTAEDIEAVLPPEELRASRRLAVFECFQDIPCDPC
                     AAACPYGAIASFDDINDRPRVEAEKCIGCAKCVSQCPGLAIFVVEEHYSAQEALVVIP
                     YEYLPVPKRNDIVAGLDREGVEVCRARVVKVKNAVAQDRTRLLWLAVPSALAHVVRNI
                     RVEA"
     gene            633..923
                     /locus_tag="VFD74_06270"
     CDS             633..923
                     /locus_tag="VFD74_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZK49187.1"
                     /translation="MPDTTAAATIICRCEDVTLEEIRAQIAAGRHTIDEIKRLTRCGM
                     GPCQGRTCRTLVAGEIARAVGVDVAEVALPIFRPPLKPVKLGVLLAEENDGA"
     gene            916..2073
                     /locus_tag="VFD74_06275"
     CDS             916..2073
                     /locus_tag="VFD74_06275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZK49188.1"
                     /translation="MRKRADAVVIGGGIHGCSVAYHLAKKGMGDVVLVEKDYLAACGT
                     GRSAAGIRHQFGTEVNVRLSAASLAMMETLSEDLDYAHGIELLQKGYLMLAYGRPELE
                     TFTRNVALQHRIIADCKTVMMTPEQIHELNPHLNLDGVLGGSFNPRDGHASPWHTTQA
                     FAEAAARLGVEINTRTEVTGIRLDGDRVGGVVTTRGEILTDTVVDCAGPFGAVVSRMA
                     GVDLPLTPQRHQILVTEPVAEVVPMMVISYTHGTYFKQTPHGSILMGYGDPAHEVIGL
                     EQDASWQFLVEVARKITYHMPILENVRVVRQWAGLYDMTPDSQAILGGVPEVRGFYLD
                     VGWSGHGFMLGPIAGKVLSEIIVGEEPTVDVDDFRYARFAENDLCPEPACV"
     gene            2156..3280
                     /locus_tag="VFD74_06280"
     CDS             2156..3280
                     /locus_tag="VFD74_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate flavodoxin/ferredoxin oxidoreductase"
                     /protein_id="HZK49189.1"
                     /translation="MIEFLEGNDAVVRAAVDAGCTFFAGYPITPATSILTGMMRALPE
                     VGGVAVQCEDEIASIGMCLGAAMTGQKALTATSGPGMSLYSENIGLAQMGETPVVIVD
                     VQRQGPATGSATKGADADIMFLRYGTSGGLPLIVLSPESVSECYSLTRLAFDLAERYR
                     TPVVLASQKEVGLTRERVDLDAARDAYGPSPARASAPPDHPYLPHAFSGPADVGPLSP
                     IGGPHLVRYTTSMHDQRAYLTSDPQVIADMMEHLRRKIEDHEADIARFDFDEEEGADT
                     LLLSYGVAARSAREAVRRVRAQGGRISLLVLKTLFPVPATALRAALAGVRRVVVAEMN
                     LGQYVQVVRAEASGPQVVSVGQMNTDLLAPDTIIREGGLL"
     gene            3277..>3530
                     /locus_tag="VFD74_06285"
     CDS             3277..>3530
                     /locus_tag="VFD74_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49190.1"
                     /translation="MSPRESAATPAAAGSDAAAVSGGIRPPSYLVDEERMPLPFCPGC
                     GHARVTRALDEALRMLGRPQERVVVVTDIGCVGLTDRHFQT"
BASE COUNT          586 a         1218 c         1162 g          564 t
ORIGIN      
        1 ggttatggtg gaggtcggaa cggcaacgag tccgaaccgc tgcgcctcgc ctaccttgag
       61 cagctgcgtg aactacgctg cggacccatg ggcgccaaga cccgtgacgg catctgcagt
      121 ctgacgagct caggaggatg atcgtgctct cgcacacagg cataccgacc gccgaggata
      181 tcgaggcggt gctgccgccc gaggagctcc gcgcttctcg gcgcctggcc gtcttcgagt
      241 gcttccagga catcccctgc gatccctgcg ccgcggcatg tccctatgga gccatagcct
      301 ccttcgacga catcaacgat cggcctcgcg tggaggcgga gaagtgcatc ggttgcgcca
      361 agtgcgtgtc gcagtgtcca ggactcgcca tcttcgtggt agaggagcat tactccgctc
      421 aggaagcgtt agtcgtgatc ccctacgagt acctgcctgt tcccaagcgg aacgacatcg
      481 tggcgggtct ggaccgagag ggcgtcgagg tgtgccgtgc gcgggtggtc aaggtcaaga
      541 acgctgtcgc ccaggatcgc actcgcctgc tctggctcgc ggtgccctcc gcgctggccc
      601 atgtggtccg caacatcaga gtggaggcct agatgcctga cacgaccgcc gcagcgacca
      661 tcatctgccg ctgcgaggac gtcacgctgg aggagatacg cgcccagatc gcagccggcc
      721 gtcacaccat agacgagatc aagcgcctga cccgctgcgg catgggcccc tgtcaggggc
      781 gcacctgccg aacgctggtg gccggcgaga tcgcccgggc agtgggagtc gatgtcgccg
      841 aggtggcgct ccccatcttc cgcccgcctc tgaagccggt gaagctgggg gtcctgctcg
      901 cagaggagaa cgacggtgcg taagcgtgcc gacgccgttg tcatcggagg gggcatccac
      961 ggctgctccg tcgcgtacca ccttgccaag aagggcatgg gcgacgtcgt gctcgtggag
     1021 aaggactacc tggccgcctg tggcaccggt cgctcggcag cgggtatccg ccatcagttc
     1081 gggaccgagg tcaacgtgcg cctgtcggcg gcgagcctgg cgatgatgga gacgctgtcg
     1141 gaggatctgg actacgccca cggcatcgag ctactgcaga agggctacct catgctggcc
     1201 tacggtcggc cggaactcga gaccttcacc cgaaacgtcg ctctgcagca tcggatcatc
     1261 gccgactgca agaccgtgat gatgacgccg gaacagatcc acgagctgaa cccgcacctc
     1321 aacctggatg gcgtcctggg cggctccttc aatcctcggg atggccacgc cagcccttgg
     1381 cacaccactc aagccttcgc cgaggcggcc gcccgtctgg gggttgagat taacacgcgc
     1441 accgaggtca ccggcatccg ccttgacggc gaccgtgtgg gcggggtggt caccacccgg
     1501 ggcgagatcc tcaccgacac cgtggttgac tgtgctgggc cattcggggc ggtcgtgagt
     1561 cgaatggcgg gagtggatct gcctctcacc ccgcaacgcc accagatcct ggtgaccgaa
     1621 ccggtggccg aggtggtgcc catgatggtc atctcctaca cgcacggcac ctacttcaag
     1681 cagacgcccc acggaagcat cctcatgggc tatggggacc ccgcgcacga ggtcatcggc
     1741 ctcgagcaag acgcgagttg gcagttcctg gtcgaggtcg cccgcaagat cacctaccat
     1801 atgccgatcc tcgagaacgt gcgcgtggtg cgccaatggg ccggtctcta cgacatgacc
     1861 cccgactccc aggcgatcct gggcggggtg ccggaggtgc ggggcttcta cctggacgtc
     1921 ggttggagcg gccacgggtt catgctgggc cccatagcgg gcaaggtgct ctctgagatc
     1981 atcgtgggcg aggagcccac agtcgacgtc gacgacttcc gctacgcgcg cttcgctgag
     2041 aacgacctgt gtcccgagcc ggcatgcgtg tagcgcaaac agtgttcgga gtaggcgtgc
     2101 acagcagacg cgagcggcag acgcacgccg gagatcgcat agagaaaggc atcgcatgat
     2161 tgaattccta gaaggcaacg acgccgtggt ccgggccgcc gtggatgcgg ggtgcacgtt
     2221 cttcgccggc taccccataa ctcccgccac ctccatcctt accggcatga tgcgggcgct
     2281 gcccgaggtg ggcggagtgg ccgtgcagtg cgaggacgag atcgcctcca tcggcatgtg
     2341 cctgggcgcg gccatgaccg ggcagaaggc actcaccgcc acctcagggc ccggcatgag
     2401 tctctactcg gagaacatcg gtctggccca gatgggggag acccccgtgg tcatcgtgga
     2461 cgtgcagcgc cagggcccgg ccacgggttc cgccaccaag ggcgccgacg cggacatcat
     2521 gttcctgcgc tacggaacct ccgggggcct gcccctgatc gtgctctccc cggagtcggt
     2581 gagtgagtgc tactccctga cccggctcgc cttcgacttg gccgagcgct accgaacccc
     2641 ggtcgtgctg gccagccaga aggaggtggg tctgacccgc gagcgcgtgg acctcgacgc
     2701 agcccgcgac gcctacggcc cctcccccgc gcgcgcctcc gctcctcccg accaccccta
     2761 cctccctcac gccttctccg ggcccgccga cgtcggcccc ctctcgccca tcgggggccc
     2821 gcacctggtg cgctacacca cgagcatgca cgaccagcgt gcctacctca cctccgaccc
     2881 gcaggtgatc gcggacatga tggagcacct acgccggaag atcgaagacc acgaagccga
     2941 catcgcccgc ttcgacttcg acgaagagga gggagccgat accctgctcc tctcctacgg
     3001 cgtggccgcc cgctcggccc gggaggccgt tcggcgggtg cgggcgcagg gcggccgcat
     3061 ctccctgcta gtcctaaaga ccctcttccc cgtgcccgcg accgcgctcc gggcggcgct
     3121 cgcgggcgtc cgccgcgtgg tggtggcgga gatgaacctg ggccaatacg tgcaggtggt
     3181 gcgggcggag gcctccggcc cccaggtcgt ctcggtgggc cagatgaaca ccgacctcct
     3241 cgcacccgac accatcatcc gggaaggagg gctgctgtga gccccagaga gtcggccgcc
     3301 acccccgcgg ccgccgggtc ggatgccgcc gccgtctccg gcggcatccg accaccctca
     3361 tacctcgtgg acgaggagcg catgcccctg cccttctgcc cgggctgcgg gcacgctcgg
     3421 gtcacccggg ccttagacga ggccctacgc atgctgggac gtcctcagga acgagtggtg
     3481 gtggtcaccg acatcggctg cgtgggcctc accgaccgtc acttccagac
//
LOCUS       DASRMJ010000221         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_491239, whole genome shotgun sequence.
ACCESSION   DASRMJ010000221 DASRMJ010000000
VERSION     DASRMJ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491239"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFD74_06290"
     CDS             <1..774
                     /locus_tag="VFD74_06290"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZK49191.1"
                     /translation="MAVTILSNESPGPAHTTDVASTAAVSSTVPDAPLTTVSLTSTTL
                     TPLQAAQAEGGYLVVEAISDPIKIYDKVPPGSVVSHSLPRNGYLDVLATFWAIDEAID
                     DQGNTWYRVYVPVRPNETKGWVKASDVSTKVLTHDVRIDLSEHRLDLYDRGTLVRSYD
                     IGVGKGETPTPLGEYFVAIKMKTPNPDGVYGVQIMGLSGFSEKLTDWPGGGQVGIHGT
                     NDPSTVGTDVSHGCIRLRNKDILELSNDVHLGTPVFVKD"
     gene            779..2176
                     /gene="mtaB"
                     /locus_tag="VFD74_06295"
     CDS             779..2176
                     /gene="mtaB"
                     /locus_tag="VFD74_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HZK49192.1"
                     /translation="MLHATRVAFRTLGCKLNQCETAQMEESLAARGLELVPWSHAADV
                     RVLNTCTVTGKTDRSCRNEIGRARRSDPESRLVVTGCYAQVAAETIAAIPGVDLVLGN
                     VDKAKLAEHIDDLLESSRKPTAPTLVTPFTADTVFAGDFVTHFSGYTRAFLKVQNGCD
                     SRCSYCVIPVARGPSRSMRMASVLDQVRLLTERGYGEVVLTGIHLGQWGRDTDEGPLA
                     HLLRALLRETSIPRLRLSSIEPLEVDEPLLSAATEAGSRFAEHFHMPLQSGSATVLSR
                     MHRPYSIEQYSERVLAVREAFPDAAIGADVIVGFPGESEEEFAETFELVTRLPLTYLH
                     VFPYSDRPGTEASRAPTKVAPEMISERSARLRALGRAKDADFQAGFAGRQLRALVLRQ
                     WSDDGRLVSLTGNYIEVLVNGDEDLVNQFAHVVPRRREEDGRWEGELLTARPGVDADT
                     ATTLFEPSGHSKARR"
     gene            2173..>2266
                     /locus_tag="VFD74_06300"
     CDS             2173..>2266
                     /locus_tag="VFD74_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZK49193.1"
                     /translation="MRAVLQRVSSAAVHVDGVEVGRIGHGLLILV"
BASE COUNT          396 a          773 c          728 g          369 t
ORIGIN      
        1 atggcggtga ccatcctcag caacgaatcg ccggggcccg cgcacaccac cgacgtcgcg
       61 tcgacggcgg ccgtttcgtc caccgtgccc gacgctcccc tcactacggt ctccctcacg
      121 agcaccacgc tgacccccct gcaggcggcg caggccgaag gaggctacct cgtggtggaa
      181 gccatctccg accccatcaa gatctacgac aaggtgcccc cgggctcggt ggtctcgcat
      241 tcgctgccgc gcaatggcta tctggacgtc ctcgctactt tctgggcgat cgacgaagcg
      301 atcgacgacc agggcaacac gtggtaccgg gtatacgtgc cggtgcggcc gaacgagacg
      361 aagggatggg tcaaagcctc cgatgtgtcc acgaaggtcc tcacccacga cgtgcgcatc
      421 gatctctcag agcaccgact cgacctgtac gatcgcggca cgctcgtccg tagttacgac
      481 atcggtgtgg gcaaaggcga gacacccaca cctcttggcg agtacttcgt cgccatcaag
      541 atgaagacac cgaaccccga cggcgtctac ggcgtgcaga tcatgggact gagcggcttc
      601 tctgagaagc tcaccgactg gccgggcgga gggcaggtgg gcattcacgg cacgaacgat
      661 ccgagtacgg taggcaccga cgtgagccac ggctgcatac ggctacgcaa caaggacatc
      721 ctggagcttt cgaacgacgt ccatttgggc accccggtct tcgtgaagga ctaacttcgt
      781 gcttcacgcc accagggtcg cctttcgcac gctcggctgc aagctcaacc agtgcgagac
      841 cgcgcaaatg gaggagtccc tcgcggcgcg aggcctggag ttggtgccgt ggtcgcacgc
      901 cgccgacgta cgcgtgctga acacctgcac cgtcaccggc aagaccgatc gctcctgccg
      961 caacgagatc ggccgcgcga ggcgctccga cccggagtcc aggctggtgg tcaccggctg
     1021 ctacgcacag gtggccgcgg agaccatcgc ggccatcccc ggtgtcgatc tggtcctggg
     1081 caacgtggat aaagccaagc tcgccgagca catcgacgac ctgctggaaa gctcccgaaa
     1141 gcccacggcg cccacgctgg tcacgccctt caccgccgac accgtcttcg ccggcgattt
     1201 tgtcacgcac ttctccggct acacgcgggc cttcctcaag gtccagaacg ggtgcgattc
     1261 tcgctgcagc tactgcgtga tccccgtcgc ccgcggcccc tcgcgcagca tgcgtatggc
     1321 cagcgtgctg gaccaggtgc gcctcctcac agagcggggc tacggagagg tggtgctcac
     1381 cggcatccat cttggacagt gggggcgcga tacggatgaa ggcccgcttg cccacctgct
     1441 gcgggcgctg ctgcgtgaga cctcgatccc ccgattgcgc ctcagctcca tcgagcctct
     1501 ggaagtcgac gaacccttac tgtccgcggc gactgaggcc gggagccggt tcgccgagca
     1561 cttccacatg cctctccaat cgggctcggc taccgtgctg agtcggatgc acaggccgta
     1621 ttccatcgag cagtactcgg agcgcgtcct ggccgtacgc gaggccttcc cggacgccgc
     1681 catcggtgcg gacgtcatcg tcggattccc gggcgagtcc gaggaggagt tcgccgagac
     1741 ctttgaactg gtcacccggc tgccgctcac ctaccttcac gtcttcccct acagcgatcg
     1801 gcccggcacc gaggcatcgc gggcgccgac gaaagtcgcg cccgaaatga tctcggagcg
     1861 aagcgcgcgg ctgcgcgcgc tgggacgtgc caaggacgcg gactttcagg caggcttcgc
     1921 cgggaggcag ttgcgcgcgc tcgtgctcag gcaatggagt gacgacggcc ggctcgtgtc
     1981 cctcaccggc aactacatcg aggtgctggt gaatggcgac gaggatctcg tcaatcagtt
     2041 cgcgcacgtg gtcccccgtc ggcgggagga ggacggtcgc tgggagggcg agctgctgac
     2101 cgcccggccc ggtgtcgatg cggataccgc aacgactctc ttcgagccga gcggccattc
     2161 gaaggcgcgg cgatgagagc cgtcctccag cgggtgagca gcgccgccgt gcacgtggac
     2221 ggcgtggagg tgggccgcat cggccacggc ctcctgatac tggtgg
//
LOCUS       DASRMJ010000222         5800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_524564, whole genome shotgun sequence.
ACCESSION   DASRMJ010000222 DASRMJ010000000
VERSION     DASRMJ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524564"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFD74_06305"
     CDS             complement(<1..569)
                     /locus_tag="VFD74_06305"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HZK49194.1"
                     /translation="MDAVDAPRGPGSRTDAPPDARDRTDAPPGTGQIATREIPTFTAI
                     ALTTRGPYWKLSGALTRLKVRLVEVGIEPRGPAFGIFYDDPSEVPPEQTRYSICFPIE
                     RPASDRSEHPLGPPTALPPAETALGSPNSVDAISLVNVPATPAAVVEYKGPAVDSPRV
                     YEHVLSWIAENAYEPSGPPRERYLAEPGT"
     gene            complement(575..1315)
                     /locus_tag="VFD74_06310"
     CDS             complement(575..1315)
                     /locus_tag="VFD74_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK49195.1"
                     /translation="MVGLRYAYPDGTRALCGIDLTIEEGESVAVIGPNGAGKSTLLLH
                     LNGILRGKGVIRVFGTPIEDSTLREVRRRVGVVFQDPDDQLFLTTVNQDVAFGPSNLG
                     LGPEEVAHRTHEALESVGMGGFGERAAHHLSFGQRKRVATATVLSMRPDILVLDEPSS
                     NLDPRSRRHLGEILAALPVTKIIVTHDLPYAYHTCERAVILSGGRVAADGPIGAILSD
                     SGLLAAHDLELPQGFAPQAAKGESSGTG"
     gene            complement(1339..2112)
                     /gene="cbiQ"
                     /locus_tag="VFD74_06315"
     CDS             complement(1339..2112)
                     /gene="cbiQ"
                     /locus_tag="VFD74_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HZK49196.1"
                     /translation="MTGTHHFLTDQRIGASPIHRLDPRAKIVGFLTLTVVGVSTPATE
                     VWAFALYYALLLFLVGLSRLPLAFVVRRAALVLPVVAVVAVFMPFFHGGGGRTFHVLG
                     GVEVTEAGLMLLSNVLLKAALGALSAIVLGLTTSFGELIVGLDGLRVPRIFTLIVSFM
                     YRYSFVFTEESRRMHRAMESRNYRGRWLWDAKVIGHMVSALFLRSYHRGERVYVAMIS
                     RGYDGTMPAASPPGIHSAEVVFVVCLTLALGAIRTGAAL"
     gene            complement(2109..3329)
                     /locus_tag="VFD74_06320"
     CDS             complement(2109..3329)
                     /locus_tag="VFD74_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HZK49197.1"
                     /translation="MHLPDGFIDAPTAVATAGGSLVVVAYAMRRAGRLMGERTVPLLG
                     VTAAFIFAAQMLNFPVAAGTSGHFVGAAFAAVVLGPWAAIVVMTVVVAVQAVGMADGG
                     ITALGANVLNMGVVAVLAGYLVHRALRRILPHSATGYLTSVGVAAWASVVISAALTSV
                     ELAVSGTVPLHLALPAMASVHAVIGLGEAVITITVLATVLASRPDLVRSGPLAGRIPG
                     SRPHAHGTPGDDMAVREATDPTDDATGRPAGDATGDATTTRRRPWGFLAVALAIAVTL
                     AVVVSPFASRAPDGLESVAEAQGFAQAGTGGWNLSPVADYRLPGISDSGLATALAGAA
                     GTIALFAIVLLLGRSLSGRRTRVVAPPAGALHPLGGHEHTDHRHAGHSHAGHTHSTEV
                     PEDREPHHSHTGAP"
     gene            complement(3335..3781)
                     /locus_tag="VFD74_06325"
     CDS             complement(3335..3781)
                     /locus_tag="VFD74_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZK49198.1"
                     /translation="MTHHATRTLRELGYRLTPQRALVWDVLRHHGGHMSAEQICERVQ
                     ESFPNVNMSTVYRTLDLLVSLHLVKETLLGPEHRTFEIEEEEAHHHLVCRECGTVEHI
                     HDGDLAGLPASLCGSSGFVATEVTIFGACSRCAASGAADESAPRAD"
     gene            4023..5090
                     /gene="selD"
                     /locus_tag="VFD74_06330"
     CDS             4023..5090
                     /gene="selD"
                     /locus_tag="VFD74_06330"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:4071..4073,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HZK49199.1"
                     /translation="MSETPPRLTTLSHGSGUACKLGPKDLGRIISQLPTFDDPALLVG
                     TQTADDAAVYRLSDDLALVQTVDLFTPVVDDPYDFGRIAAANSLSDIYAMGATPLIAL
                     NIVGFPADILPMEYLALMLQGGADVAREARMTIVGGHTIDDREPKYGLAVTGTVHPNA
                     YVTNATAEPGNVLVLTKPLGTGVVATAIKRGVADPETIAAATLSMTTLNRDAAEAALT
                     AGVTSMTDVTGFGLLGHLLEMCDGSGVGAEVWFDALPWLPGAVDLAANGVVPGGTRRN
                     LEFVDSHVEWRGAFDEHERLLAADAQTSGGMLIAVPPERVEGLVADLRSRGTLAAAVV
                     GRIREVSGPSIVVCRAPGGCE"
     gene            5087..>5800
                     /locus_tag="VFD74_06335"
     CDS             5087..>5800
                     /locus_tag="VFD74_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK49200.1"
                     /translation="MSSAPASIPGGRAAEATGAAVGRAKWLALGVVMVGTFMGPLDGS
                     VVNIALPTLTRHFSVGITTVEWVVVAYLLTISTLLLTFGRLGDMIGLKPLYLTGFAVF
                     VAGSAACAFSWSIWALVGFRVVQAVGAGMLFAVGPAIVTQVFPPSERGKALGLVGVSV
                     SAGLAIGPTLGGLLIAAGGWRLVFLINLPIGIAAFSLAWKVLHLEKPRTQRFDPYGAA
                     LSFLALFPLLLALSKGEAWG"
BASE COUNT          911 a         1906 c         2050 g          933 t
ORIGIN      
        1 aaggtgcccg gctccgcgag gtagcgttcg cgcggcggtc ccgacggttc gtaggcattc
       61 tccgcgatcc aggagagcac atgctcgtag acccggggcg agtcgaccgc cggtcccttg
      121 tactccacga cggcggccgg cgtcgccggc acgttcacga gcgagatggc gtcgaccgag
      181 ttgggcgagc cgagagccgt ctccgcaggt gggagtgctg tcggcggtcc gagagggtgt
      241 tcggaccggt ctgacgcggg gcgctcgatc gggaagcaga tgctgtagcg ggtctgctcc
      301 ggcggcactt ctgaggggtc gtcgtagaag atgccgaagg ccggcccgcg cggctcgatc
      361 ccgacctcaa cgaggcggac cttgagtctc gtcaaggcac ccgagagctt ccagtacggt
      421 ccgcgcgtcg tcagcgcgat ggcggtgaag gtcgggatct ccctggtcgc gatctggccg
      481 gtgcccggcg gtgcgtccgt gcggtcgcgc gcgtccggcg gagcgtccgt gcggctacct
      541 ggtcctcgcg gtgcgtccac ggcgtccacg cgccctaccc ggtcccggag gattcgccct
      601 tcgcggcctg aggagcgaac ccctggggca gttcgagatc gtgcgcggcg agaagtccgg
      661 agtccgagag gatcgcgccg atggggccgt cggccgcgac gcgaccccct gacaggatca
      721 cggcccgttc gcaggtgtga tacgcatagg gtaggtcgtg ggtcacgatg atcttcgtga
      781 ccgggagggc ggccagtatc tctcccaggt gtctgcgcga gcgcgggtcg agattcgaac
      841 tcggctcgtc gagcaccagg atgtccggcc gcatgctgag gacggtggcc gtggccaccc
      901 gcttcctctg gccgaaggag aggtggtgtg cggcccgctc tccgaaaccg cccatcccga
      961 cggactcgag ggcttcgtgg gtccgatgcg cgacctcctc ggggcccagc cccaagttcg
     1021 acgggccgaa ggcgacgtcc tggttcacag tggtgaggaa cagctggtcg tcggggtcct
     1081 ggaagaccac gcccacgcgg cgtcgcacct cgcggagggt ggagtcctcg atgggggtgc
     1141 cgaacacccg gatcacaccc ttgccccgga gtatgccgtt caggtggagc agtagcgtcg
     1201 acttcccagc tccgttcggg ccgatcacgg ccacggactc gccctcctcg atggtcagat
     1261 cgatcccgca gagggctctg gtcccatcgg ggtaggcgta gcgcagaccg acgatctaga
     1321 ttgcgggagt tcgggggctc acagggccgc tccggtgcgg atcgctccca gcgccaacgt
     1381 gagacacacc acaaagacga cctctgccga gtggatgccc ggcggggagg ccgcgggcat
     1441 cgtgccgtcg tagcccctgc tgatcatggc gacgtagacg cgttcccccc ggtggtagct
     1501 acgcaggaag agcgcactca ccatgtgtcc gatgaccttg gcgtcccaaa gccagcgacc
     1561 ccgatagttg cgcgactcca tcgcccggtg catgcggcgc gactcctcgg tgaacacgaa
     1621 gctgtagcgg tacatgaacg agacgatcaa cgtgaagatg cgtggcaccc tgagaccgtc
     1681 gagccccacg atcaactcgc cgaagctggt ggtcaagcca agcacgatag ccgagagcgc
     1741 gcccaaggcg gctttgagca ggacgttcga caggagcatg aggccggcct cggtgacttc
     1801 gaccccgccc agcacgtgga acgtgcgccc cccgccgccg tgaaagaacg gcatgaacac
     1861 ggcgacgacg gccaccaccg gcaggaccag cgccgccctt cgcacgacga aggcgagcgg
     1921 cagtcgcgac aggcctacca ggaagagtag cagggcgtag tagagtgcga acgcccacac
     1981 ctcggtggcg ggagtggaga cccccaccac agtcagagtg aggaagccga cgatcttcgc
     2041 gcggggatcg agtcggtgga tcggactcgc tccgatgcgc tggtcggtga ggaagtggtg
     2101 ggtcccggtc atggcgcgcc ggtgtgggag tggtggggct cgcgatcctc cggcacctcg
     2161 gtcgagtgcg tgtgacctgc gtgtgaatgg ccggcgtggc ggtggtcggt gtgttcgtgt
     2221 ccccccaggg gatgtagagc cccggcgggt ggcgccacca cgcgggttcg ccgcccgctc
     2281 aggctgcgtc ccaggagaag aacgatcgcg aacagagcga tggtcccggc cgcgcccgcc
     2341 agtgcggtcg cgagtcctga gtcagagatg cccgggagac gatagtcggc caccggggag
     2401 aggttccatc cgcccgtgcc tgcctgcgcg aacccctggg cttcggctac gctctccaac
     2461 ccgtcgggcg cccgcgaggc gaacggcgag acgacgacgg caagagtcac agcgatcgcc
     2521 aacgccactg cgaggaagcc ccatgggcgg cggcgggtcg tcgtggcgtc acccgtggcg
     2581 tcacccgctg ggcggccggt ggcgtcgtcc gtcggatcgg tcgcctcgcg taccgccatg
     2641 tcgtcgcccg gggtgccgtg cgcgtgcggc ctggatcccg gtatccggcc ggcgagcggg
     2701 ccgctccgca ccaggtcggg gcgcgaggcc aggaccgtcg ccagcactgt gatcgtgatg
     2761 acggcctctc cgaggccgat gaccgcgtgg accgaggcca tagccgggag ggcgaggtga
     2821 aggggcaccg tgccgctcac tgccaactcc acggaggtaa gcgccgccga gatgaccaca
     2881 gacgcccatg ccgccacgcc gacactcgtc aggtagcccg tcgccgaatg cggaaggatg
     2941 cgccgcaacg cgcggtgcac gaggtagccc gccaacaccg ccacgacccc catgttgagc
     3001 acgttggctc ccaaagccgt gatgccgccg tctgccatgc cgacggcctg cacggccacc
     3061 accaccgtca tcacgacgat ggcggcccac ggccccagga cgacggcggc gaaggcggcc
     3121 cccacgaagt ggccgctcgt cccggccgcc accgggaagt tcagcatctg cgccgcgaag
     3181 atgaatgcgg cggtcacgcc gagcagaggg acagtgcgtt cacccatgag tcgccccgcg
     3241 cgccgcatgg cgtaggcgac gaccacgagg gaaccgccgg ccgtggccac cgcggtgggg
     3301 gcgtcgatga atccgtcggg aagatgcatg gcggtcagtc cgctctcggc gcgctctcgt
     3361 cggcggcgcc cgacgcggca caccgggagc aggcgccgaa gatagtcacc tcggtggcca
     3421 cgaagccgct ggatccgcac agtgacgcgg gcaggccggc caagtcgccg tcgtggatgt
     3481 gctcgacggt gccgcactcc ctgcacacca gatggtgatg tgcctcttcc tcctcgatct
     3541 cgaaggtccg atgctcgggg ccgagcagcg tctccttgac caaatgcaga ctcaccagga
     3601 ggtcgagagt gcggtagacc gtggacatgt tcacgttggg gaaggattcc tgcacccgct
     3661 cgcagatctg ctccgcgctc atgtggcccc cgtggtgccg cagcacatcc cacacgaggg
     3721 cgcgctgagg ggtgaggcgg taacccaact ctcggagggt gcgggttgcg tggtgagtca
     3781 ttgcaattgc aagtctattg cagatacgtc tcggtgccaa gtcttccgtc ttggtgcgga
     3841 accacaccga gtccgccgta ccgcggcctg cttggcgagc cccatccttg tctggtagtt
     3901 tggagcaccg gcaccagcgc ccaagccggc gcccgcatgg gcaccggcac gggcatcagc
     3961 acctgcgcag cggcactgcg ccggactgga tcgtttccac cgccactgac gaaggaggaa
     4021 tgatgagcga gacgcccccg agactcacca cgctttcgca cggttccggc tgagcgtgca
     4081 aactgggccc caaggacttg gggcgaatca tctctcagtt gcccaccttc gacgatccgg
     4141 ccctgctcgt gggtacgcag acggcagacg atgccgccgt gtaccgcctc agtgatgatc
     4201 tagcgctggt gcagacggtt gacctgttca cccccgtggt ggatgacccg tacgacttcg
     4261 gccgcatcgc cgccgccaat tcgctctccg atatctacgc gatgggtgcg acgcctctga
     4321 tcgccctcaa catcgtgggc tttccggcgg acatcctgcc catggagtac ctcgcactca
     4381 tgttgcaggg cggggcggac gtggcgcgcg aggcccgcat gacgatcgtc ggggggcata
     4441 ccatcgacga tcgcgagccg aagtacggac tcgctgtcac ggggacagtc cacccgaacg
     4501 cctacgtcac gaatgcgacg gctgaacctg ggaacgttct cgtgctgacc aaaccgctgg
     4561 gcacgggcgt cgtggccacc gccatcaaac gtggcgtggc cgatcccgag acgatcgccg
     4621 ctgcgacgct gtccatgacc accctgaacc gtgacgctgc cgaggcggcg cttaccgccg
     4681 gggtcacgtc tatgaccgac gtcacagggt tcggcctact ggggcatctg ctcgagatgt
     4741 gcgacggcag cggggtggga gctgaggtgt ggttcgacgc gctgccatgg cttcccggag
     4801 ccgttgacct cgccgcaaac ggcgtcgtgc cgggaggcac ccgcaggaac ctggagttcg
     4861 tcgactctca cgtcgagtgg cgaggcgcgt tcgacgaaca cgagagactt ctggccgccg
     4921 acgcacagac ctcgggaggg atgctgatag ccgtgccgcc ggaacgggtg gaagggctcg
     4981 tggcagacct gcgttcgcgt ggcactctgg cggcggcggt ggtcgggagg atacgagagg
     5041 tctccgggcc gtccatcgtc gtctgccggg cgccgggcgg ctgcgagtga gctcggctcc
     5101 cgcctcgata cccggcggtc gtgcggctga ggcgacgggc gctgcagtgg ggcgggcgaa
     5161 gtggcttgcg ctcggggtgg tcatggtggg cacgttcatg ggtccgctgg acggcagcgt
     5221 cgtcaacatc gcactgccca ccctcacacg gcacttctcc gtgggcatca ccaccgtgga
     5281 gtgggtggtg gtggcctacc ttctcaccat cagcaccctt cttctgacgt tcggtcggtt
     5341 gggggacatg atcggcctca agccgctcta cttgaccggc ttcgccgtgt tcgtcgcggg
     5401 ctctgcagcc tgcgccttct cctggagcat atgggcgctg gtcggctttc gcgtcgtgca
     5461 ggcggtgggc gcgggcatgc tctttgcggt cggcccggcg atagtcactc aggtgtttcc
     5521 tccctcggag cgggggaagg ccttgggtct cgtgggcgtg tcggtgtcag cggggctggc
     5581 tatcgggccc actctcggcg ggctgctgat cgcggcgggc gggtggcgcc tcgtgttcct
     5641 catcaacctg ccgatcggca tcgcggcctt ctccctcgcc tggaaggtgc ttcacttgga
     5701 gaagccgcgc acgcagcgct tcgatccgta cggtgcggcg ctctcgtttc tggcgctgtt
     5761 cccgctgctg ctggcactgt cgaagggcga agcgtggggg
//
LOCUS       DASRMJ010000223         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_420211, whole genome shotgun sequence.
ACCESSION   DASRMJ010000223 DASRMJ010000000
VERSION     DASRMJ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420211"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VFD74_06340"
     CDS             complement(<1..1195)
                     /locus_tag="VFD74_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HZK49201.1"
                     /translation="MKATEAKLLDFLKKSPQFVIPIYQRTYSWTERECRQLWDDILRT
                     GSRDDIAAHFVGSIVYIEKGLYQVSSQSPLLVIDGQQRLTTVMLVLEALARRLGDTEP
                     VDGFSAKKLRNYYLLNPLEEGERGFKLLLTQTDKQSLLALMQQKSKPAQHSLRVAENF
                     CFFEEQVLALGGDLTPLCNGLAKLVIVDIALSRDQDNPQLIFESMNSTGRELSQADLI
                     RNFILMGLEPAHQTRLYEDHWRPMEVAFGQEAYGSHFDGFMRHYLTLRTGEIPNVKAV
                     YEAFKAHARTTEVAAAGVNALVADIHAYAGYYCSMALDGEHDMELAVAFRDLRELKVD
                     VAFPFLLELYQDFAERRLVKDDFVKAVRLVEAYVFRRGICAIPTNSLNKTFATFGRVL
                     KKDRYL"
     gene            complement(1192..3174)
                     /locus_tag="VFD74_06345"
     CDS             complement(1192..3174)
                     /locus_tag="VFD74_06345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HZK49202.1"
                     /translation="MTNGDVGWIANFIWGIADDVLRDLYVRGKYRDVILPMTVLRRLD
                     AVLEPTKGNVLRMKDALDEAGVVHQDPALRTAAGQAFYNTSKFTLRGLRARASQSQLK
                     ADFEAYLDGFSPNVQDILENFEFRNQIPRLSKADALGTLIEKLLSPDINLGPNPVLKG
                     DGSVKHPGLDNHAMGTIFEELVRRFNEENNEEAGEHWTPRDAVKLMAKLIFLPIAEEI
                     ESGTYLLYDGACGTGGMLTVAEETLGELAAAHGKEVATHLYGQEINSETYAIAKADLL
                     LKGDGEAADNIVGGPEYSTLANDAFPSREFDFMLSNPPYGKSWKSDLERMGGKGGLKD
                     PRFLIEHAGNPEYSLVTRSSDGQMLFLANMLSKMKQGTKLGSRIAEVHNGSSLFTGDA
                     GQGESNIRRWIIENDWLEAIVALPLNMFYNTGIATYIWVLSNRKPEHRRGRVQLIDAT
                     QWFKPLRKNLGKKNCELSDEDIQRICDTFLAFKETEQSKIFPNAAFGYWKVTVERPLR
                     LRGIDPYRVYAAKEIKALKETAERADDAPPVVKKIHKRGVEPDPRRGLFSATIGGKPA
                     VVEYESDSDLRDTEQVPLLEEGGIEAFLRREVLPYAADAWYVPDSVKTGYEVSFTRYF
                     YKPQPLRSLEEIRADILALEKETEGLLSEIIGGNAS"
BASE COUNT          545 a         1009 c         1153 g          667 t
ORIGIN      
        1 ccaggtagcg atccttcttg agcacgcggc cgaaggtggc gaacgtcttg ttgagcgagt
       61 tggtggggat ggcgcaaatc cccctgcgga agacgtaagc ttcgaccagg cggacggcct
      121 tgacgaagtc gtccttcacg agccgccttt cggcgaagtc ttggtacagc tccaggagga
      181 agggaaacgc cacgtccacc ttcagctcgc gcaggtcgcg gaaggcgacc gccagctcca
      241 tgtcgtgctc cccgtcgagc gccatcgaac agtagtagcc cgcgtaggcg tggatgtcgg
      301 cgaccagggc gttcacgccg gccgcagcga cctcggtcgt gcgcgcgtgg gccttgaagg
      361 cctcgtagac ggccttgacg ttggggatct cgccggtcct gagcgtcaga tagtgacgca
      421 tgaagccgtc gaagtggctc ccgtaagcct cctggccgaa ggcgacctcc atcggccgcc
      481 aatggtcctc gtagagcctc gtctggtggg cgggctccag gcccatcaag atgaagttgc
      541 ggataaggtc ggcctggctc agctcgcggc cggtcgagtt catgctctcg aagatgagct
      601 gcgggttgtc ctgatcgcgg ctgagtgcga tgtcgacgat caccagcttt gccagcccat
      661 tgcacagtgg cgtgaggtcg ccgccgagcg ccagcacctg ctcctcgaag aaacagaagt
      721 tctcggcgac acgcagcgag tgctgggcgg gtttcgactt ctgctgcatc agcgccaaca
      781 ggctctgctt gtcggtctgg gtgagcagca gcttgaagcc gcgctcgccc tcctcgagcg
      841 gattgagcag gtagtagttg cgcagcttct tggccgagaa gccgtccacc ggctcggtgt
      901 cgcccaggcg ccgggccagg gcttcgagga cgagcataac cgtggtcagg cgctgctggc
      961 cgtcgatgac gagcagcggc gactggctcg acacctgata cagccccttc tcgatgtaga
     1021 cgatggaacc gacgaaatgc gccgcgatgt cgtccctact gcccgtgcgc agaatgtcgt
     1081 cccagagttg gcggcactcg cgctcggtcc aactgtacgt gcgctggtag atcgggatga
     1141 cgaactgcgg cgacttcttg aggaagtcga ggagcttcgc ttcggtggct ttcacgacgc
     1201 gttccccccg atgatctcgc tcaacaaccc ctccgtctcc ttctccaggg ccaagatgtc
     1261 cgcccgaatc tcctccaaag agcgtaacgg ctgcggcttg tagaagtagc gggtgaagct
     1321 gacctcgtag ccggtcttga cgctgtccgg gacgtaccag gcgtcagcgg cgtaagggag
     1381 cacctcgcgg cgcaggaagg cctcgatgcc gccctcttcc aggagcggca cttgctcggt
     1441 gtcgcgtagg tcggagtcgg actcgtactc gacgactgcc ggcttgcctc cgatcgtggc
     1501 cgagaacagg ccgcggcgag ggtcgggttc gacgccccgt ttgtggatct tcttgacgac
     1561 aggcggcgcg tcgtcggcgc gctcggcggt ctccttcagt gccttgatct ccttggcggc
     1621 gtagacgcgg tagggatcga tgcctcggag ccgcaagggt cgctccactg tcaccttcca
     1681 gtagccgaag gcggcgttgg ggaagatctt gctctgctcg gtctccttga aggcgaggaa
     1741 ggtgtcgcag atgcgttgga tgtcctcatc cgagagctcg cagttcttct tgccgagatt
     1801 cttgcgcagc ggcttgaacc actgcgtcgc gtcgatgagt tgcacccggc cccgcctgtg
     1861 ctccggcttg cggttgctga gaacccagat gtaggtggcg atccccgtgt tgtagaacat
     1921 gttgagcggc agggcgacga tggcctcgag ccagtcgttc tcgatgatcc agcggcggat
     1981 gttgctctcc ccttggccgg cgtctccggt gaagagagag gagccgttgt gtacctcggc
     2041 gatgcggctg ccgagtttcg tgccctgctt catcttgctc agcatgttgg ccaggaagag
     2101 catctggccg tcgctggagc gggtgacgag cgagtactcc gggttgcctg cgtgctcgat
     2161 gaggaagcgc gggtctttga ggccgccctt gccccccatg cgttccaggt cgctcttcca
     2221 gctcttaccg tagggcggat tggagagcat gaagtcgaac tcgcgggagg ggaaggcgtc
     2281 gttggcgagg gtggagtact ccggtccgcc gacgatgttg tccgcggcct caccgtcccc
     2341 tttcagcagg aggtcggcct tggcgatggc ataggtctcg gagttgatct cctggccgta
     2401 gaggtgggtg gccacctcct tgccgtgcgc ggcggcgagc tcccccaggg tctcctcggc
     2461 cacggtcagc atgcccccgg tgccacacgc cccgtcgtag aggaggtaag tgccggactc
     2521 gatctcctcg gcgataggca ggaagatcag cttggccatg agcttcacgg cgtcccgggg
     2581 cgtccagtgc tctcccgcct cctcgttgtt ctcctcgttg aagcggcgca ccaactcctc
     2641 gaagatggtg cccatggcgt ggttgtcgag tccggggtgc ttgacggagc cgtccccctt
     2701 gagaacgggg ttcggaccta ggttgatgtc gggcgagagg agcttctcga tgagggtgcc
     2761 gagggcgtcg gccttggaga gtcgcgggat ctgattgcgg aactcgaagt tctctaggat
     2821 gtcttggacg ttcggggaga agccgtcgag gtaggcctcg aaatcggctt tgagttggct
     2881 ctgactggcc cgagcccgga gaccgcgcag cgtgaacttg gaggtgttgt agaaggcctg
     2941 accggcggcg gtgcggaggg ccgggtcctg gtggaccacc ccggcctcgt cgagagcgtc
     3001 tttcatgcgg agcacgttgc ccttggtggg ttccagcacg gcgtccaggc ggcggagcac
     3061 cgtcatgggc aggatgacat cgcgatactt gccacgaacg tagaggtcgc ggagcacgtc
     3121 gtccgcgatt ccccagatga agttcgcgat ccagccgacg tcaccgttgg tcatgcgtac
     3181 ttcctccctg ggcgtgacgg tcgtgtctgc ccgcgtgggc acgttgcggg ttcggcatcg
     3241 ggttcagcga cgaagagcag cgtaccctcg acgcaggggt caccgattgc tcgaaagcaa
     3301 cgtcaatcgt acagcccgga acggatagac ggaagtgata cggggacggc gggacccagc
     3361 ggacagtgtg ctac
//
LOCUS       DASRMJ010000224         4828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_238463, whole genome shotgun sequence.
ACCESSION   DASRMJ010000224 DASRMJ010000000
VERSION     DASRMJ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238463"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..284)
                     /locus_tag="VFD74_06350"
     CDS             complement(6..284)
                     /locus_tag="VFD74_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49203.1"
                     /translation="MPLQGTLFDASCRAVHGIVIPGRRIIDGFQVSQDRRILQFSAQV
                     RLEAFNQLVPPCDSPSAGDHHMERHELASSRPAGAQGMVFHSVLFVRL"
     gene            complement(1121..1209)
                     /locus_tag="VFD74_06355"
     tRNA            complement(1121..1209)
                     /locus_tag="VFD74_06355"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Ser,seq:cga)
     gene            complement(1374..1808)
                     /locus_tag="VFD74_06360"
     CDS             complement(1374..1808)
                     /locus_tag="VFD74_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZK49204.1"
                     /translation="MDSPPVCVDASFILRLFMGPDEETAWRLWDSWLARGSSIHSPAL
                     LGYEIANALHRYRRAGFLSLSSADLVLDAALSLPTRLHSGRELHARALRLAADLSLPA
                     TYDAHYLAVAEQFGAELWTADARLAAQVPDGPPPVGLLGRDG"
     gene            complement(1808..2110)
                     /locus_tag="VFD74_06365"
     CDS             complement(1808..2110)
                     /locus_tag="VFD74_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZK49205.1"
                     /translation="MQRVITATEARVHFGDLLRRVVEDKETILVERGGSPQVVVLSVA
                     EYERLLSHRASEGWRDRVVVARERVRSDLAGRTVPRPEDVIDEGRSQRDEQPTHPR"
     gene            complement(2221..3072)
                     /locus_tag="VFD74_06370"
     CDS             complement(2221..3072)
                     /locus_tag="VFD74_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZK49206.1"
                     /translation="MEITSRTVDHNALLDLILESSPMGITVLTTDLRYVLVNRYAREE
                     LLKLPDEETLGKHCYDLFGMYANDAARSGLERACDACPALRALSTGKPAANVRRVREG
                     LVVQVTSVPLTDDTGDYIGVMEMTENIADKVIDPLTGVHNFRFYDEMMTQEGYRAQRY
                     DTTLALIALDLNNFKLVNDRYGHLRGDEVLREVAATLLRTVRNSDLLCRIGGDEFAVL
                     APHTTYHEAEALARRIEQSVDGEFHVLGVSISSGIAGYPRDTLNPAELRAIADRRLYE
                     IKDRHRG"
     gene            complement(3078..>4828)
                     /locus_tag="VFD74_06375"
     CDS             complement(3078..>4828)
                     /locus_tag="VFD74_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK49207.1"
                     /translation="QYITEQRDSEDALRESERRSAVRNRIAQVFLTAGGQGDGVVFVR
                     VRDVILEAVQSRYGILGYVDGGGSWVCPGVAADPPLESGTPRGDLVLAGREWSGVWGR
                     AMRERRTVVETGVVETPGGSRVRRALDMPIISGEQLLGNILIGDRDAEFAEKDVRFVE
                     SVAEYLAPIFHARLQLAEQERASRQLQDQLQQAQKMEAIGRLAGGVAHDFNNMLGVIL
                     GYGETLLEGLPDGDPLRDYVVEMVAAGERSATMTRQLLAFSRTQTLRPEVVDLNAVVE
                     GSVRLLRRVIGEDVTLEFVPAATPVPVRLDAGRWEQVLMNLAVNARDAMRGGGTLTIE
                     VKIVEFDASSALANLGIEPGYYAVLDIADTGCGMDSATEARVFEPYFTTKESGRGTGL
                     GLSSVYGTVEQSGGTIWVHSEVGRGTTFTIHLPMAEATIAIRPAEAPVERRPRVGRGE
                     LVLVVEDEDPLRRLVQAILLRLGYTVAVAANGNEAVELVEGAELKPELVITDVVMPGM
                     NGVALAKRLRKTLPDLKVIFMSGYTDAEIASSLDVDARWWSFISKPFTKEALAGTIEA
                     ILRDEDDPVPGVPSTE"
BASE COUNT          803 a         1623 c         1539 g          863 t
ORIGIN      
        1 ggattctaga ggcgtacaaa caggacggag tggaatacca tgccctgcgc acccgccggt
       61 cgggagctcg ccagttcgtg tcgctccatg tgatggtccc cggcgcttgg actgtcgcac
      121 gggggcacca actggttgaa cgcctcgagt ctgacctgtg ccgagaattg caggatgcgg
      181 cggtcctgac tcacttggaa cccatcgatg atccggcgtc ctgggatgac gatgccgtgg
      241 accgctcggc atgaggcatc aaagagagtt ccttggagag gcattgggca cgttcgtcct
      301 cgcgctgttg cgtgggggat ctgagtgacc ctcgcgacgg tgttcggcgg acggatgcaa
      361 cgccgcacat tgccggcgtc gtcgcgagtg aggcgcggcc tattggacgt cgcctccact
      421 cggcttgttc ggctcagtcc gattttttct cttccgactg gcgggatgga agaaattggc
      481 acgccgcaga agctacgagt ccgaccacaa gcccgacgac gccacacccg cggcgcccgt
      541 gcctggtgag gaaactgcag ccgcgagtcc gccggacaca ccacctatat cctttctgtg
      601 gggtctgaca accaatatcc agtccgcatg actccaccga cacatcaccc ggctgtctgc
      661 cctatcagtg aagcggcggc gggatacgga cccgcaaacg cccgccagca cggcaagagt
      721 cccgatgagg cgagaccgcg tctcctggat gcccagacgg ataggcagcc ctaccagccg
      781 tcaagcgggc tgctgacgcc catgctgccc cccactatcg atcgcgaagg tcgcgtcgcc
      841 agccggtcat gacctccgac ctccaccctg agaggagggg atccccctcg actgtccggt
      901 tggcggtcaa gacgaagcct cgatctgttg aggctgaccc ctggggacgt caagagagcc
      961 gccctgtctg gtccgtccag tccgtctccg tccgtctagt ggtgacgtgt ttggccccaa
     1021 accgccacca agatcctcgc caccgccccc aagaatgcaa gagcccggct atggcgaccg
     1081 ggctcctcct catgatttac ctgcaaaata ggctagaagg cggagagggg gggattcgaa
     1141 cccccggtac ctcttgcgag gcacaacggt tttcgagacc gccgcattca accgctctgc
     1201 cacctctccg tggccgcccg ccaccggaag gacgtcgatc tcgacgccgt ccggcgggcg
     1261 agcaccgatg atacccgatg cgtcgcccgg catccacgtg tgcgccgtca gggtgttcgc
     1321 gcggtccgta cgccatgatt gcgcacgtgg gtctccgacc cgtatcactc ctgtcagccg
     1381 tcgcggccca acaggcccac cggaggcggt ccgtcaggga cctgcgccgc gagccgtgcg
     1441 tcggcggtcc acagctctgc accgaactgc tctgccacgg caaggtagtg ggcgtcgtac
     1501 gtcgcgggga gggacagatc ggcggcgagg cggagcgcgc gcgcgtgcag ttcccggccc
     1561 gagtggaggc gcgtcggcag tgagagagct gcgtccagga ccagatctgc cgaagataag
     1621 ctgaggaaac ccgcccgccg gtagcgatga agagcgttcg ctatctcgta gcccaacagg
     1681 gcggggctgt ggatgctcga tccccgcgcg agccacgagt cccacaggcg ccacgcggtc
     1741 tcctcgtcgg gacccatgaa caggcgaagg atgaagcttg cgtcaacgca gacgggtggg
     1801 ctgtccatca gcgaggatgg gtgggctgtt catcgcgctg tgagcgccct tcatcgatga
     1861 cgtcctcggg gcgggggaca gtccgaccgg cgaggtccga ccgcacccgc tcgcgggcga
     1921 cgacgactcg gtccctccaa ccttcggatg cgcggtggga aagcagccgt tcgtactcgg
     1981 ccaccgacaa caccaccacc tgcggactac cgccgcgctc gactaggatg gtctccttgt
     2041 cttcgaccac ccggcgcagc aggtcgccga agtgcacccg ggcctccgtc gctgtgatca
     2101 cgcgttgcat ctcgacactc ctaggtggcg cgttggtcga tcaaaccatc gattgatcat
     2161 aacaccttgc gatccccggg acttgccgtt cctttcggtg gctgcctgct ccacgcgggc
     2221 tcagcctctg tggcgatcct tgatctcgta gaggcgccgg tcggcgatgg cgcgcaactc
     2281 cgccgggttc agcgtgtcac gggggtagcc ggcgatgccc gaagagatgg agacccccaa
     2341 gacgtggaat tccccgtcca ccgactgctc gatgcgtcgc gcgagggctt cggcctcgtg
     2401 gtaggtggtg tggggagcaa gcacggcgaa ctcgtcgccc ccgatgcggc agagaaggtc
     2461 actgttgcgc acggtacgca agagcgtggc ggcaacctcg cgaaggacct cgtctccccg
     2521 caggtggccg tagcggtcgt tcaccagctt gaagttgttg aggtcgaggg cgatgagggc
     2581 gagcgtcgtg tcgtagcgct gagcgcggta gccctcctga gtcatcatct cgtcgtagaa
     2641 gcggaagttg tgtaccccgg tgagtgggtc gatcaccttg tcggcgatgt tctcggtcat
     2701 ctccatgacg ccgatgtagt cgcccgtgtc gtcggtcagg ggaaccgacg ttacctgcac
     2761 caccagcccc tcgcgcacgc ggcgcacgtt ggcggcgggc ttccctgtcg agagggcgcg
     2821 cagggcgggg cacgcatcgc aggcccgttc caggccgctg cgggcggcgt cgttggcgta
     2881 catgccgaac aggtcgtagc agtgctttcc caacgtctcc tcgtcgggaa gcttcagcag
     2941 ttcctcgcgg gcgtagcggt tcaccaggac gtagcgcagg tcggtggtga gcacggtgat
     3001 gcccatgggc gaactctcca ggatgagatc caggagcgcg ttgtgatcga cggtgcggga
     3061 ggtgatctcc atcgtgacta ttccgtcgag gggacgccgg gcaccgggtc gtcctcgtcg
     3121 cgcaggatcg cctcgattgt tccggcgagc gcctccttcg tgaagggctt ggagatgaac
     3181 gaccaccacc gcgcatcgac gtcgaggctg ctcgcgatct ctgcgtccgt gtaccctgac
     3241 atgaagatca ccttgaggtc ggggagtgtc ttgcggaggc gcttcgccag cgcgacgccg
     3301 ttcatccccg gcatgacgac gtcggtgatc acgagttcgg gcttcagttc cgcgccttcc
     3361 accagctcca cggcctcgtt tccgttcgcg gcgaccgcca ccgtgtatcc caatcgaagg
     3421 aggatggcct ggacgagtct gcgcagcggg tcttcatctt ccaccaccag caccaattct
     3481 ccgcgcccga cgcgcggcct acgctcgacc ggcgcttcag cgggtcggat cgcgatcgtc
     3541 gcctcggcca tcggaagatg gatggtgaac gtcgtcccgc gaccgacctc cgagtgcacc
     3601 catatggtgc cgcccgattg ctcgacggtg ccgtaaactg aggagaggcc caacccggtg
     3661 cctctgcccg actccttggt ggtgaaatag ggctcgaaga cccgagcttc ggtcgcggag
     3721 tccatgccgc atccggtgtc ggcgatgtcg agtacggcgt agtatccggg ttcgatcccc
     3781 agattcgcca acgcggacga ggcgtcgaac tccacgatct tgacctcgat ggtcagcgtg
     3841 ccgccaccgc gcatggcgtc gcgggcgttg acagcgaggt tcatcagaac ctgctcccac
     3901 cttccggcgt cgaggcgcac gggaaccggc gtggcggcgg gcacgaactc cagcgtcacg
     3961 tcttctccga tgaccctgcg gagcagcctc acggagcctt ccaccaccgc gttcagatcc
     4021 accacttccg ggcgcagggt ctgcgttcga ctgaaggcca gcagctgtct ggtcattgtc
     4081 gccgaacgct cgccggccgc gaccatctcc accacgtagt ctcggagcgg atcaccgtcg
     4141 gggagtccct cgagcagtgt ctctccgtac ccgaggatga cgcccagcat gttgttgaag
     4201 tcgtgagcca cgccgccggc gaggcggccg atggcctcca tcttctgggc ctgctggagc
     4261 tgatcttgga gttgccgact cgctctctcc tgctccgcca actgcagccg agcatggaag
     4321 ataggtgcca ggtattcggc gacgctctcg acgaatcgca cgtccttctc tgcgaactcg
     4381 gcgtctcggt cgccgatcaa gatgttcccc agcagctgtt cccccgagat gatcggcatg
     4441 tccagcgccc gccgcacgcg cgatcccccc ggtgtctcga cgaccccagt ctctacgacc
     4501 gtccttcgct ccctcatagc ccggccccag acgccgctcc actcgcgacc tgccaggacg
     4561 aggtctcctc ggggcgtgcc cgactccagc ggaggatccg ccgccacgcc ggggcatacc
     4621 cagctgccgc ccccgtcgac gtatcccagg atcccgtatc ggctctgcac ggcctccagg
     4681 atgacatccc tgacccgcac gaagacgacg ccgtctcctt gacctccggc ggtaaggaag
     4741 acctgggcga tgcggttcct gacggccgac cggcgctccg attctctcaa ggcgtcctcg
     4801 gaatcccgct gctcggtgat gtactgta
//
LOCUS       DASRMJ010000225         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_138030, whole genome shotgun sequence.
ACCESSION   DASRMJ010000225 DASRMJ010000000
VERSION     DASRMJ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138030"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..542
                     /locus_tag="VFD74_06380"
     CDS             75..542
                     /locus_tag="VFD74_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006721999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HZK49208.1"
                     /translation="MTTLLIDADACPVTRPAISIARGRGIPVVLVSNGSQNLGRYADR
                     RGVEVLQVTSGPDAADFAIVGRLRSDDVVVTQDIGLAAMVLGKGARALSPRGRLYRPE
                     TIDAEMAVRYAEQKHRRAGGRTRGPDPFVEEDRERFGEMLARLLDEAQAGGES"
     gene            complement(858..1928)
                     /locus_tag="VFD74_06385"
     CDS             complement(858..1928)
                     /locus_tag="VFD74_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZK49209.1"
                     /translation="MEPESGLLLVDPSGSRWLSSRDLPDILGRLRTPQAPVTWLHAAV
                     SDDLVAALEAAGLHELAVKSLRDGPERPRVEEYPDHLYVSLLRARDGEALESASAASA
                     RPAASAGPPAAAATGASPDASVDRHTIQIEDIRVFLGSRWIISLGGLDQDELATVSAR
                     VSRQVMERGRGPSFVLYYLAEWAVGTLYPLLDTLDDRVDSLEDLVLVDTGDTTRQELF
                     RLKRDLAESRRRVAPTRDVMQRLSTHGLGFVEPGVEVFFRDVHDDVLLVLEQLDTHRD
                     ILSSALDLYLSTVSNRLNEVMKRLTVVATVFMPLTFITGFFGMNFVDLPVGSTAWYGL
                     TLAGMVVLSAAMVGYAWRRRWL"
BASE COUNT          415 a          766 c          739 g          307 t
ORIGIN      
        1 ggtgtgcggc gtaggtggga tgatgggcgt gaccatcagt ggacaataca ccacagaacg
       61 aggcggttga ccacatgacc acgctattga tcgatgccga cgcgtgcccc gtgacgcgcc
      121 ccgcgatctc catagcccgg ggccggggca tccccgtcgt gctggtgagc aacggctccc
      181 agaacctcgg caggtatgcc gaccgccgag gcgtggaggt gctccaagtc acctcgggcc
      241 ccgacgccgc cgacttcgcg atcgtcggcc gtctccgctc cgacgacgtg gtggtgactc
      301 aagacatcgg tctcgcggcc atggtgctgg gcaagggcgc ccgcgcgctc agtcctcgcg
      361 gcagactcta ccggcccgag accatcgacg ccgagatggc agtgcgatac gcggagcaga
      421 agcaccgccg cgcgggcggg cgcacccgcg gtccggatcc cttcgtggag gaagacaggg
      481 agcggttcgg cgagatgttg gcccgcctgc tggacgaagc gcaggcgggc ggcgagagct
      541 agttcgaaag ccgggccggc gagcgggggc gccgcatgga gtgagccggt cgaccagcgc
      601 acggtgcccg cgaccaccag gcctccggcc agcagcacga acacgtaggt gggcagggcg
      661 aaggcggccc cggattcttg gagtccccgc agattgacca gagccaccag agcgatgagt
      721 gcgacggcca atcttcttgg acaggcgttc ctccttctcc tgggccgtcg ccaacggcct
      781 tccgagtaga ctccgcttga ggaacacgaa cgaagacgtc atctaccgct cccgctgcct
      841 ttccgtcgac tcgatgctca cagccagcga cgacgccatg cgtagccgac catcgccgcg
      901 ctgagcacca ccatccccgc cagcgtcaac ccgtaccacg ccgtactgcc caccggcagg
      961 tcgacgaagt tcatgccgaa gaaccctgtg atgaaggtga ggggcatgaa cacggtggcg
     1021 acgaccgtca gcctcttcat cacctcgttc aagcggttcg agaccgtcga gaggtagaga
     1081 tcgagggccg aggagaggat atcgcggtgc gtgtcgagct gctcgagcac aagcaggacg
     1141 tcgtcatgca cgtctcggaa aaagacctcc acgcccggct ccacgaagcc caagccgtgc
     1201 gtcgacaggc gctgcatgac gtcacgagta ggcgcgacgc gacgcctcga ctccgccagg
     1261 tcgcgcttga gacgaaagag ctcctgccgc gtggtgtccc ccgtgtcgac caggacgagg
     1321 tcctcgagcg agtccacccg gtcgtcgagc gtatcgagca gagggtatag ggtccccacc
     1381 gcccactccg ccaggtagta caggacgaag ctcggccccc tgccccgctc catgacctgc
     1441 cgcgacacgc gagcgctcac cgtggccagt tcgtcctgat cgagcccgcc caaggagatg
     1501 atccagcgtg agccgaggaa cacgcggatg tcctctatct ggatggtgtg acggtcgacg
     1561 gaggcgtcgg gggacgcacc tgtagctgcg gcggctgggg ggccggctga ggcggcgggt
     1621 cgggctgagg cggccgaggc gctctcgaga gcctcgccgt cgcgcgcccg cagcagagag
     1681 acgtacaagt ggtcgggata ctcctcaacc cgcggccgct cggggccgtc gcgcagactc
     1741 ttcaccgcca actcgtgcag gcccgccgcc tcgagcgcag ccacgagatc gtcggagact
     1801 gcggcgtgca accacgttac gggcgcctgg ggcgtgcgca gccgccccaa gatgtcggga
     1861 agatcacgcg aagacaacca tctcgaaccc gaagggtcga ccaataggag acccgactct
     1921 ggctccaccg atatcggact tctcgacacc gagccccccg tttcgtgagg acttccagga
     1981 accgacatac ccttacccct cccgtgaggc cgctggtcac cacggagagg atacgcgccc
     2041 attggcgctt gcgctatgcc ggccgcgcga tggactcggt cacggaaaag caccccgggc
     2101 gctgcgcagg cacgcccacg ggctgcggac gcacgcccac gtgaagcccc tcaccacacc
     2161 cgcacgcacg cctgcgggaa gacaatagaa aaaggggcgg gtgggctcgc gcccacccgc
     2221 cccgcag
//
LOCUS       DASRMJ010000226         9314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_162524, whole genome shotgun sequence.
ACCESSION   DASRMJ010000226 DASRMJ010000000
VERSION     DASRMJ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9314
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162524"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFD74_06390"
     CDS             complement(<1..290)
                     /locus_tag="VFD74_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019377419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK49210.1"
                     /translation="MTHSKADLVIRDAAQVVTCVGEPYDVGVVADAWIAITGETIAAV
                     GSRAEVEAAVDCSGAGVVDARGRVVVPGFVDAHTHLVFGGSRVEEYALRMTV"
     gene            complement(287..2002)
                     /gene="hutU"
                     /locus_tag="VFD74_06395"
     CDS             complement(287..2002)
                     /gene="hutU"
                     /locus_tag="VFD74_06395"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZK49211.1"
                     /translation="MGRPEILYSVRAQRGPKLRCKGWKQETILRMLENNMENAEKPEE
                     LVIYGGIGKCARNWESYHATVDALLNLEDDETLVMQSGMPVAVFQTHKLAPRVVMANT
                     NIMKADWPTFYDLQDKNLTMFAQYTAGPWEYIGTQGVIQGTFETLSAIARKHYDDSLV
                     GRILLTAGAGGMGGNQTRAMTMHGGVGIVVDADERVIDRRIEKKFTDVKVGSLAEAVS
                     LAQKSAAAGEALGIAVVGNAADLFEEALTMDFRPDMVTEMCPCHDPISYIPSGYSPDE
                     ASALRTADRARYLELARETMIRQLRAMNRFFALGVPTFEYGTSIRKECRDAGMPADEA
                     MTIPGFVAEYIRPLFCEGRGPFRWTCVSGEASDLARLDDLCMELFADDLLVTRWISLA
                     REHIPIEALPARICYLGFGQRKRFGLAVNELIKKGEVKGPVAFSRDNLDSGSIVNPTF
                     ESENMLDGGDLISDWPMLNGLLNAVGMCDLIAQQANYSMGEAVHTGVTMIADGTDEAD
                     FRLSVCLTVDSGIGIVRHAQAGYAAAKDVANGKGALTDESIKVPLWWTPKATFGPKDL
                     ETTGQ"
     gene            complement(2042..3616)
                     /gene="hutH"
                     /locus_tag="VFD74_06400"
     CDS             complement(2042..3616)
                     /gene="hutH"
                     /locus_tag="VFD74_06400"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZK49212.1"
                     /translation="MTVHIDGGSLTIEDAVRVARHGERVEVTPSARERMERAREVIEE
                     VLSGGAVVYGVNTGFGKFSDVVIPEDQLDLLSKNLIVSCCVGVGRPFDTEVVRAMLLL
                     RANSLAVGNSGVRPLVVDGLVEMLNKRVHPVVPQKGSVGSSGDLAPLSHLAVVLLGGG
                     KAVYEGEVLPGAVALQRAGMEPLRLKAKEGLSLSNGTQAMTAVGALAVYDAERLADTA
                     DIAGALSAEALEAVPFAFDERIHKSRPHVGQSTTARNLRRLLDGSDILAGATHGRLQD
                     AYALRCMPQVHGASRDAIAYVKSVIETEINSVTDNPLVFADEHEVISGGNFHGQPVAL
                     AMDFLTIALAELANISERRTERMMNPALSGGLPAFLIEEGGINDGFMVAQYTAAALVS
                     ENKVLAHPACVDSIPTSANQEDHVSMGTISARQAREVLENVEHVLAIELMCACQGVEF
                     RARKPAAPAQRVCALIREKVDRLEADREMDLDIEAVAALIREGRLLDAAAQPADSGHG
                     WSESDDGHARPGSQRP"
     gene            complement(4040..4567)
                     /locus_tag="VFD74_06405"
     CDS             complement(4040..4567)
                     /locus_tag="VFD74_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HZK49213.1"
                     /translation="MAAITLRGCGVVAGVVEGEALVCPTSIQGWGGIDPATGVIKEHG
                     HVNRGVSIKGRILVLPGSRGSNGWSCYFGAANVAGAGPLGMVVTRIDSSVGVAAAVMQ
                     VATVVDFASNQDPCRLIATGDRVRVDGGSGVVEIVKVAATPGTDARGQEAAEKRSLGP
                     QNALGGAIRRPRSRT"
     gene            complement(4549..5850)
                     /locus_tag="VFD74_06410"
     CDS             complement(4549..5850)
                     /locus_tag="VFD74_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HZK49214.1"
                     /translation="MLRLTEYEEGVLAGKEGRLKQVSLQNIVRYAKILGAAELCEVTK
                     ATVFCGAHNYLDVCRSEDIDEVFSRMNLAVEESIPFDRTYGNSYIQSCVSPCDQDEHE
                     PFGQSPEFFAKNALYLEQARKAGVIIIGSCAPYLTGWLPVRGEHFVTTESSVTLMGNS
                     LWGAMGNSDGIEAAFWSAICGRTPKWGNHVEENRAGTHLVEVQAEIDGILEFDLLGRA
                     IGMRLPSGAVPVISGRFGSVTFQKLRQLFTSLAVSSNCEMSHVVGHTPEARTVEDAFR
                     GREPAGTLMIDERAMVESYQAACDPGAGEIDLVSLGCPHYDIDQIKRVAVYLEGRRVH
                     PGVHFMVWTAYPIKAMADLNGYTRIIEEAGGHIYTGSCPCTIGPTFLDKYSGCVFDSV
                     KQAVAVKSETARAVYLGDLQRCIDAAVAGRWEEEHRWPRSR"
     gene            complement(5900..7789)
                     /locus_tag="VFD74_06415"
     CDS             complement(5900..7789)
                     /locus_tag="VFD74_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZK49215.1"
                     /translation="MTVMRTLGDTLRVDLAARSVVRRPLEDAVVRRVLLGRGLNSLTL
                     LEEIGADVGPLDPANPLMFTCGLLTGTQAPSAARVQVSARSPLTGLLGSSSVGGTVGP
                     ALRANGVQSLRVVGRADSPSYLYIADGDAEVRDAARLWGLDVRETASAIEDELGERGS
                     VMFLIGPAGEHLLPLACIVTQRGHAAGRTGMGAVMGSKNLKAVVVKSAKVAAGVEKVA
                     CAGEDTREAVKRYLAKIKSSPNFTDKAAHGTSSAVNPVNEMGMLGSYNYQSPQFGAAA
                     AIDGTNMDQYVERLRACQRCPVHCKAELRLTSGRYAGLEGERPDFEPIVSWGSKSGLA
                     DPEAIIYLHHLCDLFGIDSVSAGNAVAFAMQLWQEGIITARDTGGLLLEWGDVAVMES
                     LVRQMAAGEGFGALLGQGVREAARVIGRGAEKYAYHVKGLELTAFDPRGASASGLGYA
                     VSNRGGDFTSVYARHEFSLTPVQAKELYGEELAADRLSPVGKAPMVKRSMIICAVLDS
                     IGLCKIPALTLVNEFDLVSEAELVSAVAGLSVTPEELFEIGERIVDIERLLNIRFGAG
                     AEDDMLPDHFLDEPMPQGPAEGNTIDLAAMLGEFYGVMGWTEQGVPSVARLRELGLEE
                     LAPAG"
     gene            complement(7786..>9314)
                     /locus_tag="VFD74_06420"
     CDS             complement(7786..>9314)
                     /locus_tag="VFD74_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK49216.1"
                     /translation="SAQAVTTTSLDQGGPGATTPLVPGGTVGGGVVLSVAADAIVELD
                     PAAATSTGDILVLHQIYDWLVEEDGGGGLRPGLAESWERSSPTAWTFTLRPNVIFQDG
                     DPFEAEDVVHTFERLRLLADSTGLHSSLADYAVIESVIAIDEGTVRFVLGRPDSDFLA
                     LLARDAAAILSDGTRDPSTEWVGTGPFVLWSYRAGEGAVLRRNPAYWARDAGGRQLPY
                     VDGLNVVFKSDPETRLAELVAGRVQFVGGLDLADIRLVDATPGLTTLRTPSNEHLSIY
                     IESGPGRVGEDVRVRRALKLGTDRAALAEAVRPSLSTPGNDSPVGPFYGERHLDEVPE
                     YNPEAAGLLLTEAGIVDGLKLVLDVPDDPEAAALAQAWAAQMEMIGVTVEIRDATSGA
                     GGGVDGATADARIARSISAPSPFDSLVHAYAVGVDSAAGIWVDADFEALVAQLAAETD
                     EILQVDLYHQIQQVLIERGPAVVPFYETVVAGAGKTLLGVSVAPYWPRTSFRTASFGS
                     "
BASE COUNT         1545 a         3250 c         2972 g         1547 t
ORIGIN      
        1 ctcacggtca tgcgcagcgc gtactcctcg acgcgggacc cgccgaacac caggtgcgtg
       61 tgagcgtcca cgaagccggg gaccaccacg cggccgcggg catcgaccac ccccgcgccc
      121 gagcaatcga ccgccgcctc cacctcagcc cggctcccca cggccgcgat ggtctcaccg
      181 gtgatcgcga tccaagcgtc ggcgacgacg cccacgtcgt acggctctcc gacgcaggtg
      241 accacctggg cggcgtcgcg gatgaccagg tcggccttcg agtgcgtcac tgccccgtgg
      301 tctccaggtc cttgggcccg aaggtggctt tgggcgtcca ccacaagggc accttgatgc
      361 tctcgtcggt gagcgcgccc ttgccgttgg ccacgtcctt ggcggccgca tatccggcct
      421 gggcgtgccg cacgatcccg atgccggagt cgacggtcag acagaccgag agccggaagt
      481 cggcctcgtc ggtgccgtcg gcgatcatcg tgacaccggt gtgcacggcc tcgcccatcg
      541 agtagttggc ctgctgagcg atgaggtcgc acatgcccac ggcgttgagc aggccgttca
      601 gcatgggcca gtcggagatg aggtcacctc cgtcgagcat gttctccgat tcgaaagtcg
      661 ggttgacgat ggagcccgag tccaggttgt cccgggagaa ggccaccggg cccttgacct
      721 cgcccttctt gatcaactcg ttcacggcca gaccgaagcg cttgcgctgg ccgaagccga
      781 gatagcagat gcgcgccggg agagcttcga tcgggatgtg ctcgcgggcc agactgatcc
      841 agcgggtcac gagcagatcg tccgcgaaca gctccatgca gaggtcgtcg agtcgggcga
      901 gatcggatgc ctcaccggag acgcaggtcc agcggaaggg gccgcgcccc tcgcagaaga
      961 gcggccggat gtactcggcc acgaacccgg ggatggtcat tgcttcgtcg gcgggcatgc
     1021 cggcgtcgcg gcactctttg cggatgctcg tgccgtactc gaaggtcggc acccccaacg
     1081 cgaagaaccg gttcatcgcc cggagttggc gaatcatggt ctcgcgggcc agttcgaggt
     1141 agcgggcgcg gtccgcggtc cgaagcgcgg acgcctcgtc gggcgagtat cccgaaggga
     1201 tgtaggagat ggggtcgtga caggggcaca tctccgtgac catgtcggga cggaagtcca
     1261 tggtcaaagc ttcctcgaac aggtcggcgg cgttgccgac cacggcgatg ccgagcgctt
     1321 cgccggcggc cgcggacttc tgcgcgagac ttacggcttc ggcgagcgaa cccaccttga
     1381 cgtcggtgaa cttcttctcg atgcgccggt cgatcacccg ctcgtcggcg tcgacgacga
     1441 tgcccacgcc accgtgcatg gtcatggctc gagtctggtt gccgcccatg ccgcccgcgc
     1501 cggcggtgag gaggatgcgg cccaccaggg agtcgtcata gtgcttgcgg gcgatggcgg
     1561 agagcgtctc gaaggtgccc tggatcacgc cctgcgtgcc gatgtactcc cacggtccgg
     1621 cggtgtactg ggcgaacatg gtgaggttct tgtcctggag atcgtagaag gtgggccagt
     1681 cggccttcat gatgttggtg ttggccatga cgacccgggg cgccagtttg tgggtctgga
     1741 acacggccac tggcatgccc gactgcatca ccagggtctc gtcgtcttcg aggttcagga
     1801 gcgcatcgac ggtggcgtgg tacgactccc agttgcgcgc gcacttcccg atgccgccgt
     1861 agatcaccaa ctcctcgggc ttctcggcgt tctccatgtt gttctcaagc attctaagaa
     1921 tggtctcttg tttccaccct ttgcagcgca acttcggtcc gcgttgcgca cgcaccgagt
     1981 agagtatctc cggcctaccc atcgactgtc cgcctcccac ttgaagtgtc ttcgtcgtct
     2041 actagggcct ctgggagcca ggccgcgcgt ggccgtcatc agactcgctc caaccgtgcc
     2101 ccgagtcggc aggctgcgcg gcggcgtcta gaaggcgacc ctcgcggatc agtgcggcca
     2161 ccgcctcgat gtccaggtcc atctcgcgat cggcttcaag tctgtcgacc ttctctcgta
     2221 tgagagcgca gacccgttga gcgggcgcag caggcttcct cgctcggaac tcgacaccct
     2281 ggcacgcgca catcaactcg atcgctagga cgtgttccac gttctccagc acctcacggg
     2341 cctgccgcgc gctgatcgtg cccatgctga cgtggtcttc ctggttggcg gaggtcggga
     2401 tggagtcgac gcacgcgggg tgcgcaagca ccttgttctc gctcacaagg gccgcggccg
     2461 tgtactgggc caccatgaat ccgtcgttga tgccgccttc ctcgatgagg aatgccggga
     2521 gtccgccgct cagggccgga ttcatcatcc tctcggtcct gcgttctgag atattcgcca
     2581 actccgccag cgcgatcgtg aggaagtcca tggccagcgc gaccggctgc ccgtggaagt
     2641 tgccgcccga gatgacctca tgctcatccg cgaacaccaa cgggttgtcg gtgaccgaat
     2701 tgatctcggt ctcgatgacg ctcttcacgt aggcgatggc gtcgcgagaa gcgccgtgga
     2761 cttggggcat gcaacgcaga gcgtaggcgt cctggaggcg gccgtgggtg gctccggcca
     2821 ggatgtcgct gccgtcgagc agacggcgca gattgcgggc cgtggtcgac tggcctacgt
     2881 gcgggcggct cttgtggatg cgctcgtcga aagcgaaagg cacggcctcc aacgcctccg
     2941 cggacaacgc gcccgcgatg tccgccgtgt cggcgagcct ctcggcgtcg tagacggcga
     3001 gcgcgccaac tgccgtcatc gcttgggtgc cgttggagag agacaggccc tccttcgcct
     3061 tgagccgaag aggctccatg ccggcgcgtt gaagggccac cgctcccggg agcacctccc
     3121 cctcatacac ggccttgccg ccacccagaa gcaccaccgc caggtgcgag aggggcgcaa
     3181 gatcaccgct tgaaccaacc gaccccttct ggggcaccac gggatggacc cgcttgttca
     3241 acatctcgac gagcccgtcg accaccaacg gcctcacgcc cgaattgccc accgccaacg
     3301 aatttgcccg cagcaggagc atggcccgca ccacttcggt gtcgaacggc cggcccaccc
     3361 ctacgcagca gctgacgatc agattcttgg agaggaggtc gagctgatcc tcggggatga
     3421 ccacgtcgct gaacttgccg aagcccgtgt tgaccccgta caccacggcg ccaccgctca
     3481 gcacctcttc gatcacctcg cgggcccgct ccatccgctc ccgcgccgaa ggggtgacct
     3541 ccacccgctc tccgtgccga gcgactcgca ccgcgtcttc gatcgtgagg ctgccgccgt
     3601 cgatatgcac ggtcatcccg ttctcctcct cgcaacaagc tctccaccaa ggcggtcacg
     3661 ccaagcccgt gggcggcgtg gcggcctgat gcccagtggg cgacgcggcc gccggccgcc
     3721 cattcgccga cccagcggcc gaatgcaaag tcaccgccgc ggccgccgaa cactcactcg
     3781 ccgactccga cccggccgac atgcgaccgg cgccggactt cccgcggcgc cggccaacat
     3841 cgatcgggcg aatcatatcc gatatgcgca tggcggagct cgaacgccgg cttcgcgggt
     3901 cgcgggtcgc aggtcgcagg tcgcaggtcg ccgctgaacg atagctgagg gttgacaggc
     3961 tgctgaagag tgacgcttcg ccttcagagc gcttgcgctg agtgcgagac aaggacgcag
     4021 gaagcgctcg tagggcgcgc tacgtgcgcg accgaggacg ccgtatcgcg ccgcccagtg
     4081 cgttctgggg gccaaggctt cgcttttcag cagcctcctg gccccgcgcg tccgtgccgg
     4141 gcgtcgcggc caccttcacg atctccacca ccccgctgcc accgtccacg cgcactcggt
     4201 cgcctgtcgc gatgagccgg caggggtcct ggttggaggc gaagtccacc acggtggcca
     4261 cctgcatgac ggcggccgcc acccccacgc tggaatcgat gcgggtgact accatgccga
     4321 gcgggccggc accggccacg ttggcggcgc cgaagtagca cgaccaccca ttcgaccccc
     4381 tgctgccggg cagcaccagg atcctgccct tgatggacac tccccggttc acgtgcccgt
     4441 gttccttgat gactccggtg gctgggtcga taccacccca accttggatg ctcgtcgggc
     4501 agaccagagc ctccccttcc acgactccgg ccaccactcc gcagcccctc agcgtgatcg
     4561 cggccatcga tgttcctcct cccacctgcc ggcaacggcc gcgtcgatgc accgctgcag
     4621 atcaccgaga tacaccgccc gcgccgtctc cgacttgacg gcaaccgcct gcttcacgct
     4681 gtcgaacacg cacccgctgt acttgtcgag gaaggtcggc ccgatggtgc aggggcaact
     4741 gccggtgtag atgtggcctc cggcctcctc gatgatgcgc gtgtacccgt tcaggtcggc
     4801 catggccttg atcggataag cggtccagac catgaagtgc accccggggt gcacccgccg
     4861 gccttccagg tatacggcga cccgcttgat ctggtcgata tcgtagtgag ggcagccgag
     4921 gctcacgagg tcgatctccc ccgcgcccgg gtcacaggca gcttggtagg actccaccat
     4981 ggcgcgctcg tctatcatca aagtgccggc cggctccctc ccacggaacg cgtcctccac
     5041 cgtgcgggct tccggcgtgt ggcccaccac gtgactcatc tcgcagttgc tggacacggc
     5101 caggctggtg aagagctgcc gaagtttctg gaaggtgacg gagccgaacc gaccggagat
     5161 gacgggcacg gcgcccgagg ggaggcgcat cccgatagcc cggcccagga ggtcgaactc
     5221 gaggatcccg tcgatctcgg cctgcacctc cacgagatgc gtgcccgccc ggttctcctc
     5281 cacgtggttc ccccacttgg gagtcctgcc gcagatcgcc gaccagaagg ccgcttcgat
     5341 cccgtcggag ttccccatgg ctccccagag cgagttcccc atcagcgtga ccgaggattc
     5401 ggtcgtgaca aagtgctccc cacgcaccgg cagccaaccc gtcaggtagg gagcgcacga
     5461 gccgatgatg atgacgccgg ccttgcgggc ctgctcgagg tagagtgcgt tcttcgcgaa
     5521 gaactcggga ctctgtccaa agggctcgtg ttcgtcctgg tcacagggag agacgcacga
     5581 ctggatgtag ctgttgccgt aggtacggtc gaacggtatc gactcttcga cggccagatt
     5641 catgcgagag aacacttcgt cgatgtcctc cgacctacac acgtcgaggt agttgtgggc
     5701 gccgcagaac accgtagcct tggtgacctc gcacagctcc gcggcgccga ggatcttcgc
     5761 gtagcgcacg atgttctgaa gggagacctg cttgagtctg ccctccttgc ccgcgaggac
     5821 accctcttca tactcggtca gtcgcaacac gtgcgcctcc ctggcccaag tccggagctg
     5881 atcgcggggg accggggtat catccggccg gagcgagctc ctccagcccc agctctcgaa
     5941 gccgggccac ggaaggcact ccctgctcgg tccagcccat gacgccgtag aactcgccga
     6001 gcatcgcggc caagtcgatg gtgttcccct ccgccgggcc ctggggcatc ggctcgtcga
     6061 gaaagtggtc gggcagcatg tcatcctcgg ccccggctcc gaagcggatg ttgagcagcc
     6121 gctcgatgtc cacgatgcgc tcgccgatct caaagagctc ttcgggagtg acggacaatc
     6181 cggccacggc gctcaccaac tcggcctcgc tcaccaggtc gaactcgttc accagggtga
     6241 gtgccgggat cttgcagagg ccgatggagt cgagcactgc gcatatgatc atgctgcgct
     6301 tgaccatcgg cgccttccct acgggagaca agcgatcggc ggcgagttcc tctccgtaca
     6361 actccttcgc ctgcaccggc gtcaggctga actcgtgccg ggcgtagacg ctggtgaagt
     6421 cgcccccccg attcgagacc gcgtagccga gcccgctggc tgaggcgccc cgcggatcga
     6481 aggccgtgag ttccagaccc ttcacatggt aggcgtactt ctccgcgccc cggccgatga
     6541 cccgcgccgc ctcgcgcacg ccctgcccca ggagggcccc gaagccttcg ccggccgcca
     6601 tctgacgcac gaggctctcc atcacggcga cgtcgcccca ctccagcagc aacccccctg
     6661 tgtcgcgcgc cgtgatgatg ccttcctgcc acaactgcat ggcgaaggcc acggcgttgc
     6721 cggccgacac ggagtcgatt ccgaagaggt cgcacaggtg gtgaaggtag atgatggcct
     6781 caggatcggc gaggccgctc ttcgaacccc aggacacgat cggctcgaag tcgggacgct
     6841 cgccctccag ccccgcgtaa cgacctgagg tgaggcggag ttcggccttg cagtgcaccg
     6901 gacagcgctg acacgcacgc agacgctcga cgtactggtc catgttcgtg ccgtcgatgg
     6961 ccgccgccgc cccgaactgc ggactctggt agttgtagct cccgagcatc cccatctcgt
     7021 tgaccggatt gacggcggat gaggtgccgt gcgccgcctt gtcggtaaag ttgggtgagc
     7081 tctttatctt ggccagatat cgcttcacag cctcgcgcgt atcttcaccc gcgcacgcga
     7141 ccttctcgac gcccgcggcg actttggcgc tcttcaccac gaccgccttc aggttcttgg
     7201 agcccatgac ggcccccatc ccggtgcggc ccgcggcatg accacgctgc gtgacgatgc
     7261 acgccagggg cagcaggtgc tctcccgccg ggccgatcag gaacatgacc gaccctcgct
     7321 cgcccagctc gtcctcgatc gcgctcgcgg tctcccgcac gtcgagtccc cagaggcgcg
     7381 cggcgtcgcg cacctcggcg tcgccgtcgg cgatgtagag atacgaggga gagtccgccc
     7441 gcccgacgac gcgcagggac tgcacgccgt tcgcgcgcag tgccggaccc accgttcccc
     7501 cgacactcga actcccgaga aggcccgtca gcggcgagcg ggcactcacc tgcacccgag
     7561 cggcggaggg ggcctgggtc ccggtgagca ggccgcaggt gaacatgagc gggttggcgg
     7621 ggtcgagggg ccccacgtcg gcgccgatct cctccagaag agtcagcgag ttcaggccgc
     7681 gcccgagcag gacgcggcgg accaccgcat cctccagggg tcgcctgacg acggagcgag
     7741 ccgccagatc caccctcaaa gtgtcaccga gcgtccgcat aacagtcatg agccgaacga
     7801 tgccgtccga aagctggtgc ggggccagta tggcgccacc gacacaccca gcagggtctt
     7861 gccggccccg gcgaccacgg tctcgtagaa aggcaccacc gccggaccac gctcgatcag
     7921 aacctgctgg atctggtggt agaggtcgac ttggaggatc tcgtcggtct ctgccgccaa
     7981 ctgggcgacc agtgcttcga agtcggcatc gacccagata cccgcggcgg agtcgactcc
     8041 caccgcgtag gcgtgcacca acgagtcgaa cggactcggg gcggagatcg accgagcgat
     8101 cctagcatct gccgtcgccc catccacccc gcctcctgcg ccggaggtgg cgtcgcggat
     8161 ctcaacggtg acgccgatca tctccatctg cgcggcccac gcctgggcca gcgccgccgc
     8221 ctcggggtcg tcggggacat cgagcaccag cttgaggccg tcgacgatgc cggcttcagt
     8281 caagaggagc ccggccgcct ccgggttata ctcgggaacc tcatcgagat gacgctcgcc
     8341 gtagaacggg ccgacggggg aatcattgcc cggcgtggac aggctggggc gcaccgcctc
     8401 ggcaagcgcc gcccggtccg tccccagctt cagggcgcgt cgcacgcgca cgtcctcccc
     8461 cacccgcccc gggccggatt cgatgtatat ggagaggtgc tcgttcgatg gagtcctcag
     8521 ggtggtcagc ccaggggtgg catcaaccag gcggatatcc gcaagatcca atccgccgac
     8581 gaactgcacg cgcccggcga ccagctcggc aaggcgcgtc tcgggatccg acttgaagac
     8641 gacgttcagc ccgtcgacgt atggcaactg ccgacctccg gcgtcccggg cccaatacgc
     8701 cggattcctc cggagcaccg cgccctcgcc cgcgcggtat gaccacaaga cgaacggtcc
     8761 cgtgccgacc cattccgtgg aaggatcgcg tgtgccgtcg ctcaggatcg cggcggcgtc
     8821 acgggccagt aaggccaaga agtcggaatc ggggcggccg agcacgaagc gcacggtgcc
     8881 ctcatcgatc gcgatcaccg actctatgac ggcgtagtca gccagcgacg agtgcaggcc
     8941 ggtcgaatcg gcgagcagtc gaagccgctc gaacgtgtgc acgacatcct cggcttcgaa
     9001 cgggtcgccg tcttggaaga tgacgttcgg cctcagcgtg aacgtccatg ccgttggaga
     9061 ggatcgttcc caactctccg cgagcccggg ccgtagcccc ccgccgccat cttcttccac
     9121 cagccaatcg tagatctgat ggagcacgag gatgtcgccg gtcgatgtgg ccgcagccgg
     9181 atcgagttcc acgatggcgt cggccgctac tgacaggact acaccccccc ccaccgtccc
     9241 gcccggcacc aacggagtag tcgcgccggg accaccttga tcgagtgagg tggtcgtgac
     9301 cgcttgcgcg gagc
//
LOCUS       DASRMJ010000227         5490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_162701, whole genome shotgun sequence.
ACCESSION   DASRMJ010000227 DASRMJ010000000
VERSION     DASRMJ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162701"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFD74_06425"
     CDS             complement(<1..1073)
                     /locus_tag="VFD74_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZK49217.1"
                     /translation="MAGFEYVRPTTLEGAVAELTQRGEGAVAVAGATDLWVNIRLGKL
                     KPEAVVSLRGIAGLDVLTAEFADGGARGSGGLAAAGSPAASGLGAAGGGLTIGAMTPH
                     GKVEDSLWVATHFPALQQGCAGLGSRQVRNVGTVGGNICNAAPCADSATALMLFDATL
                     VLQSTAGLRELPLGEFFVGPGMTRLLQGELLKEIRVPDPGPNTFSRYIKHTRRRGVEL
                     AMMSVGVRLTLEDDLRTVKTARISLGVCAPTPTCATRAQDMLVGRSLTREAVEAAALV
                     AADDSRVRDSWRGKAWYRREMIRVLVPRAVEQSGAYLAAGIAPAAGSGAGPSTGAGAA
                     AGDAGAPGFVGRGIDRGAGAPDEA"
     gene            complement(1132..2430)
                     /locus_tag="VFD74_06430"
     CDS             complement(1132..2430)
                     /locus_tag="VFD74_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZK49218.1"
                     /translation="MRSFLPAFETEEALGRAQLAGLRWTATHAYEGSAFYRRRFDAAG
                     VTPADIVTLDDLRRLPFTTAHDLQEDYPFPLLSVPHDQLVRIHSSSGTTGRRKILGYT
                     QKDVDDWADMFARCYEMAGVGPGDRVQIMVGYGLWTAGVGFQAGCERAGAMAVPVGPG
                     NLELQMQFLQDVQSNVICCTASMALLLAEELAARDLIGKTNIRTVILGSEVCGDAMRA
                     RILELTGADHLYDITGMTELYGPGTGLDCHYHAGIHYWADYFLLEILDPETLQPVPEG
                     EIGEMVVTTLRKEGAPLVRYRTRDLTRLIPGRCECGSVLPRHDRLMGRSDDMFIFRAV
                     NIYPGQIESILSKTDGVSCEYNVRLTRADGKDQMTVLAERDPGADPATDAAIRRRISD
                     SIKKEIMVTAEVEVVDYASLPRTERKSKRVYDEREIQTKD"
     gene            complement(2427..4208)
                     /locus_tag="VFD74_06435"
     CDS             complement(2427..4208)
                     /locus_tag="VFD74_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZK49219.1"
                     /translation="MTGSAAAGGAATVDGVTIDRVLVSILQKRLKSITEEMSIAMTRT
                     TRSPILCEAKDFVTGLYDAEGNMLEQTENLPILAFSLAPVCKYIIDYYGDDIYPGDVI
                     FHNDVFSMGNQNNDVAVYKPIFFGDTLVAWAAVKGHQADIGGNVQGGYNPLATEVWQE
                     TLRIPPVKVYEKGKLRRDVWEFIFANIRLDIVEEDMRAEIGSCVVGERGVLRILERYG
                     LEVFEAHKKFLFDSTERMMRQEIRSIPNGVYTGEANAYYDGKTPGSVYTIRVKVTVED
                     GHITFDYTGTDGQSPGFVNGTYASSASATILTFLQMVNPDIPHNQGMVRPITITIPVG
                     TILNAAYPAATTYGNHLCPNNADAIMRALGPVIPERVTAEWGELLCSLTTGKDPRKGG
                     EAFVDINFMGMKGGSGGIYGADGYDHIGMIDASGGVLDQDYEMFEQQTPHLLVKHELW
                     RDSAGAGRWRGGVGVEMEYKVGGMVNLVTFGDGDVEPSRGAQGGREGTLNIIKLTSPE
                     GVESVPTTKDLVPNVPAGTVYFQQAGGGGGWGDPHERPAEKVLRDVRNGLVSLEKARE
                     DYGVVVDTATWTVDEPATAKLRGAQLR"
     gene            4220..4510
                     /locus_tag="VFD74_06440"
     CDS             4220..4510
                     /locus_tag="VFD74_06440"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49220.1"
                     /translation="MGSDPKAAFKGMRRAYLPNEKRFADIEVYDGMELHFGNRLEGPV
                     IIEQVNTTTFVTPEFSVVVDQLGTYTIYLREREEEVLERVLGKHAATAGGVA"
     gene            4510..>5490
                     /locus_tag="VFD74_06445"
     CDS             4510..>5490
                     /locus_tag="VFD74_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZK49221.1"
                     /translation="MSNDVTGAAGVTVVDGVTIDRVLVSILQKRLKSITEEMSIAMTR
                     TTRSPILCEAKDFVTGLYDAEGNMLEQTENLPILAFSLAPVCKYIIDYYGDDVHPGDV
                     IFHNDVFSMGNQNNDVAVYKPICFGDTLVAWAAVKGHQADIGGNVQGGYNPLATEVWQ
                     ETLRIPPVKVYEKGKLRRDVWEFIFANIRLDIVEEDMRAEIGSCVVGERGVLRIVERY
                     GLEVFEAHKKFLFDSTERMMRQEIRSIPEGVYVGESNAYYDGKTPGSVYTIRVKITVD
                     DGHITFDYTGTDGQTPGFVNGTYASSASATILTFLQMVNPDIPHNQGMVRP"
BASE COUNT          906 a         1845 c         1809 g          930 t
ORIGIN      
        1 gcctcatccg gggcaccggc gccgcggtca ataccgcgcc cgacaaagcc cggggcgccg
       61 gcgtcacctg ccgcggcgcc cgcgccggtc gaagggccgg ccccgctgcc cgcggccgga
      121 gcgatgcccg cggccagata ggcgcccgac tgctccaccg cccggggcac gagcacccgg
      181 atcatctcgc ggcggtacca ggccttgcca cgccagctgt cgcgcacccg tgagtcgtcg
      241 gcagccacca gggccgccgc ctccacggct tcgcgcgtga gggacctgcc caccagcatg
      301 tcctgcgcgc gcgtggcgca ggtgggggtg ggggcgcaga cgcccagcga gatgcgggcc
      361 gtcttgacgg tgcggaggtc gtcttcaagg gtcaggcgaa cgccgacgct catcatggcc
      421 aactcgacgc cgcggcgccg cgtgtgcttg atgtagcggg agaaggtgtt cgggccgggg
      481 tcgggcaccc gtatctcctt gagcagttcg ccctgcaaca agcgcgtcat gcccgggcct
      541 acgaagaact cgcccagcgg caactcgcgg aggccggcgg tgctctgcag caccagggtg
      601 gcgtcgaaga gcatgagggc cgtggccgag tcggcgcatg gggcggcgtt gcagatgttg
      661 ccgcccacgg tgcccacgtt gcgcacctgg cgtgaaccca agccggcgca gccctgttgg
      721 agggcgggga agtgggtcgc gacccagaga gaatcctcca ccttgccgtg gggggtcatg
      781 gcgccgatgg tcaggccgcc acccgcggcg cccaggccgc tcgccgcagg cgagccggcc
      841 gccgcgaggc cgccagagcc gcgggcgccg ccgtcagcga actcggcggt cagcacgtcg
      901 agacccgcga tgccgcgcag cgacaccacc gcctcgggct tcagcttgcc caacctgatg
      961 ttcacccaca gatcggtggc tccggccacc gccacggcgc cttcgccccg ctgggtcagt
     1021 tcggccaccg ctccctcaag ggtggtaggc cgcacatact cgaatccggc catctgctac
     1081 gcgccccttt cgtctccgtc tcgcgcctcg gcgcctcgcc cggcagtcac gtcagtcctt
     1141 ggtctggatc tcgcgctcgt catacacgcg tttcgatttc cgctctgtgc gcgggagcga
     1201 ggcgtagtcc acgacctcga cctccgcggt caccatgatc tccttcttga tgctgtcact
     1261 gatgcgacgt cggatagcag cgtcggtcgc cgggtcggcc ccagggtcgc gctccgcaag
     1321 aaccgtcatc tggtccttgc cgtcggcccg cgtgagacgg acgttgtact cgcatgacac
     1381 cccgtcggtc ttcgaaagga tgctctcgat ctgacctgga tagatgttga ccgcccggaa
     1441 tatgaacatg tcgtcggagc ggcccatcag tcggtcgtgc cgcggtagca ccgaaccgca
     1501 ctcgcaccga ccgggaatca ggcgcgtcag gtcgcgggtg cggtagcgca ccagaggagc
     1561 gccttctttg cgcagcgtgg tcaccaccat ctcgccgatc tcgccctcgg gcacgggctg
     1621 caaggtctcc ggatcaagga tctcaagaag gaagtagtcg gcccagtagt ggatgcccgc
     1681 gtggtagtgg cagtccagtc cggtgccggg cccgtagagt tcggtcatgc cggtgatgtc
     1741 gtagaggtga tcggcgccgg tcagctccaa gatgcgggcc cgcatggcgt cgccgcacac
     1801 ctcgctgccc agtatgaccg tgcgaatatt ggtcttgcca atgagatcgc gtgcggccaa
     1861 ctcctccgcc agaagcaacg ccatgctggc cgtgcaacag atgacgttcg actgcacatc
     1921 ctgcaggaac tgcatctgca gctcgagatt gcccggtccc accggcacgg ccatggcgcc
     1981 ggcgcgctcg cagccagcct ggaaacccac tccggccgtc cacagtccgt agcccaccat
     2041 gatttgcacc cggtcgcccg gacccacgcc cgccatctcg tagcagcggg cgaacatgtc
     2101 ggcccagtcg tccacgtcct tctgcgtgta gcccaggatc ttgcgacgac cagtcgtccc
     2161 ggaggaagaa tgtatgcgca ccagctggtc gtgcgggacg ctcagcaggg gaaaggggta
     2221 gtcctcctgc aggtcgtgag cggtggtgaa gggaagacgg cgcagatcgt cgagcgtcac
     2281 gatgtcggcg ggcgtcacgc cggccgcgtc gaagcggcgg cggtagaagg ccgacccctc
     2341 gtaggcgtgg gtcgccgtcc aacgcagccc ggcgagctgg gctcggccga gcgcctcttc
     2401 agtctcgaac gcgggcagaa acgatctcat ctgagctgtg ccccccgtag cttggcggtc
     2461 gcgggctcgt cgacggtcca ggtagcggtg tccacgacca cgccgtagtc ctcgcgggcc
     2521 ttctccaacg acaccaggcc gttgcggacg tctcgcagaa ccttctccgc cggccgctcg
     2581 tgagggtcgc cccatccgcc gccgccgccc gcctgctgga agtagaccgt gccggccggc
     2641 acgttcggca ccaggtcctt ggtcgtgggg acggactcga ccccctcggg ggaggtgagc
     2701 ttgatgatgt tgagcgtgcc ctccctgccg ccctgggcgc cccgcgacgg ttccacgtcg
     2761 ccgtcgccga aggtcaccaa gttgaccatg ccgcccacct tgtactccat ctccaccccc
     2821 acgccgccgc gccagcggcc ggcgccggcg gagtcccgcc agagctcgtg cttgaccagc
     2881 aggtgtgggg tctgctgctc gaacatctcg tagtcctggt cgagcacgcc gccggaggcg
     2941 tcgatcatgc cgatgtggtc gtagccgtcg gcaccgtaga tgcctcccga accgcccttc
     3001 atgcccatga agttgatgtc gacgaaggcc tcgccgccct tgcgcgggtc cttgcccgtg
     3061 gtgagactgc agagcagttc gccccactcg gccgtgaccc gctcagggat aaccggcccc
     3121 agcgcccgca tgatggcgtc ggcgttgttg ggacagaggt ggttgccgta ggtggtggcc
     3181 gccggatagg ccgcgttcag gatggtcccc accgggatgg tgatggtgat gggccgcacc
     3241 atgccttgat tgtgggggat gtcggggttg accatctgca ggaaggtcag gatggtggcc
     3301 gaggcgctcg aggcgtaggt gccgttgacg aagcccgggc tctgcccgtc ggtgccggtg
     3361 tagtcgaagg tgatgtggcc gtcctccacc gtgaccttca cccggatcgt gtacacgctg
     3421 cccggagtct tgccgtcgta gtaggcgttg gcctcgcccg tgtacacgcc gttcgggatg
     3481 gagcggatct cctgacgcat catgcgctcg gttgaatcga acaggaactt cttgtgagcc
     3541 tcgaagacct caagaccgta acgctccaag atacgaagca cgccccgctc gcccaccacg
     3601 caggagccga tctcagcccg catgtcctcc tcgacgatgt ccaggcggat gttggcgaag
     3661 atgaactccc acacgtcgcg gcgcagcttg cccttctcgt acaccttgac cggcgggatg
     3721 cgaagcgtct cctgccagac ttccgtagcc agagggttat agccgccctg gacgttgccg
     3781 ccgatgtcgg cctgatggcc cttcaccgca gcccacgcca ccagcgtgtc gccgaagaag
     3841 atgggcttgt acaccgccac gtcgttgttc tggttgccca tggagaagac gtcgttgtgg
     3901 aagatcacgt cgcccggata gatgtcgtcg ccgtagtagt cgatgatgta cttgcacacg
     3961 ggagccaggc tgaaggccag gatgggcagg ttctccgtct gctcgagcat gttgccctcg
     4021 gcgtcgtaga gcccggtgac gaagtccttg gcctcgcaca agatcgggga ccgggtggtc
     4081 cgcgtcatgg cgatggacat ctcctcggtg atgctcttca agcgcttctg caggatgctc
     4141 accagcacgc ggtcgatggt cacgccgtcg accgtggccg caccgcctgc ggcggccgag
     4201 cctgtcaccc gaagtctagc tgggctccga cccaaaggcg gctttcaaag gcatgcgccg
     4261 ggcctatctg cccaacgaga agcgtttcgc cgatatcgag gtctacgacg gtatggagct
     4321 gcacttcggc aaccgcctgg aggggccggt gatcatcgag caggtgaaca ccacgacctt
     4381 cgtcaccccg gagttctccg tggttgtgga tcagttgggc acgtacacga tctaccttcg
     4441 cgagcgcgag gaggaagtgc tcgagcgcgt cctcgggaag cacgccgcga ctgcgggagg
     4501 ggtggcctga tgagcaacga cgtgacaggc gccgcgggcg tcaccgtggt cgacggggtg
     4561 accatcgacc gggtgctggt gagcatcctg cagaagcgcc tcaagagcat cacggaggag
     4621 atgagcatcg ccatgacgcg gaccacccgg tccccgatcc tgtgcgaagc caaggacttc
     4681 gtcaccgggc tctacgacgc cgaaggcaac atgctcgagc agaccgagaa cctgcccatc
     4741 ctggccttca gtctggcgcc ggtgtgcaag tacatcatcg actattacgg tgacgacgtg
     4801 catcccggcg acgtcatctt ccacaacgac gtcttctcca tgggcaacca gaacaacgac
     4861 gtggccgtgt acaagcccat ctgcttcggc gacacgctgg tggcgtgggc cgcggtcaag
     4921 ggccaccagg ccgacatcgg cggcaacgtg cagggcggct acaatccgct ggccaccgag
     4981 gtgtggcagg agacgctccg catcccgccc gtgaaggtgt acgagaaggg caagctgcgc
     5041 cgcgacgtgt gggagttcat cttcgccaac atccgcctgg acatcgtcga ggaggatatg
     5101 cgggccgaga tcggctcctg cgtggtgggc gagcgtggag tcctgcgtat cgtggagcgc
     5161 tacgggctcg aggttttcga ggcccataag aagttcctgt tcgactccac cgaacgcatg
     5221 atgcgccagg agatccgttc gatccccgag ggtgtgtacg tgggtgagtc gaacgcctat
     5281 tacgacggca agaccccggg cagcgtgtac acgatccgag tgaagatcac cgtggacgac
     5341 ggccacatca cgttcgatta caccggcacc gacgggcaga ctcccgggtt cgtcaacggc
     5401 acctacgcct cgagcgcctc ggccaccatc cttaccttcc tgcagatggt caatcccgac
     5461 attccccaca accagggcat ggtgcggccc
//
LOCUS       DASRMJ010000228         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_163049, whole genome shotgun sequence.
ACCESSION   DASRMJ010000228 DASRMJ010000000
VERSION     DASRMJ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163049"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            564..1730
                     /locus_tag="VFD74_06450"
     CDS             564..1730
                     /locus_tag="VFD74_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZK49222.1"
                     /translation="MPRADRTGSRHSALHSTRHSALGSAARTAALSGTLAAGYMLFES
                     QWLLARENSLHVPRLPPSLEGLSILHLSDVHAGQPGFNLRTLEKAVGWAVERRPDLAV
                     LTGDILGDRSGRARCLALLGRLQPRLGIFAVPGNHEYGLSKNPLTHLPEPIPWESCGV
                     RELRDSCVMIDVPALRSAEVDPSIAAAPGASGGPAARSASGPAATADESGGDPAATAD
                     GRPAAGAARVALCGADYLTGGAPLAHQSMPLEADLRLLLVHRPPTADDPLATMFDLAF
                     SGHTHGGQIRIPTPWGLRSPHNDHVSHLEGVRAWGNGLLVVSAGIGTTFLPLRLFTRP
                     QVVIHRLTSAPSHAAEARDAFQLRREADTGESPSGIYEDVGMAHDGGGATNASG"
     gene            1717..2118
                     /locus_tag="VFD74_06455"
     CDS             1717..2118
                     /locus_tag="VFD74_06455"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZK49223.1"
                     /translation="MPADEQPAGGVIVPPDDPAEENRAHVSEDVVATYVAAAVRSIPG
                     VSELHGKPWQELSERVHMNTARKGVVIKEVAPGVVEVEVHVSVAWGTVIPEMAQRLQE
                     SVAHSVETLLDLQVRKTTVYVDEVEAPLELR"
     gene            2189..2731
                     /locus_tag="VFD74_06460"
     CDS             2189..2731
                     /locus_tag="VFD74_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49224.1"
                     /translation="MWWQTTQGPAEGRERRLEWMRLLETSWGSVGLLAVEDRETVASI
                     QFAPVRSLARPHSRPFGRPPEEAVLLFCLRGRVGRPTVETQELLHRAMSALRRRGVRE
                     VYGYARPLDTHTLSGVRNLFGLEFLEANGFVVARAIDHGYLMRADLGGLIPALSEAGG
                     SMRRRLAGAGHPSPATFWRP"
     gene            2817..>3004
                     /locus_tag="VFD74_06465"
     CDS             2817..>3004
                     /locus_tag="VFD74_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49225.1"
                     /translation="MKRPAPHLAVLTLALAVALLLTAGVADFGPRVAFAADQGRAPVW
                     VTRVEGAVVPPLSAYLVRT"
BASE COUNT          449 a         1130 c          967 g          458 t
ORIGIN      
        1 cccgtgagcc gcccgccctt cgtcggtccc cgcgagtggc tccgccgcct ccttgcccgc
       61 cgatcccatg gccgagaaac aggccagcca agcctcctcg ttttcgtcca actcccttgc
      121 gaaccgctcg cgcaccacgc acctcactcc tcgtctccgg ccactgcctc gaccacgacg
      181 ccgacgcgca cctctcgtca tcacactcac ggtgtcgacg ccggggcgca tcggttgcgg
      241 ctcgcaccag acccgttcgt caacggtacc acgcgctcgc cacgcggggc gcggccgcgg
      301 gagcgcagcc gccaggcaca ctttgctgcc gccagaacgg gccgcgagac gcgccgtggc
      361 cccggaacgc ggccgccagg cacgccgtga ccccggaacg ccaccgtccg gtatccttgg
      421 agaagtgcac gtaggacatt ggggggagcg aagatcgacc ggaaccgccg ccgaccgagc
      481 gagcatgctg tacgagccca gcaagactcc ggcgtggccg cggccctcac cgcatgagcc
      541 ggctacgccc tcgcggaaat gcgttgcccc gagccgaccg caccggtagc cggcactccg
      601 ccctgcactc cactcggcac tccgccctgg gctccgccgc ccggaccgcc gcgctctcag
      661 gaacactggc cgcgggatac atgctgttcg agtcccagtg gttgctcgcc cgcgagaact
      721 cgctgcacgt tccccggctc ccgccatccc tcgaaggctt gtcgatactc cacctgtcgg
      781 acgtccacgc cggccagccc gggttcaatc tccgcacgct cgagaaggcc gtgggttggg
      841 ccgtcgagcg ccggcccgac ctcgccgtac tcaccggcga catcctcgga gaccgatcag
      901 gcagagcccg ctgcctggcg ctcctcggtc gtctgcagcc ccgtctcggc atcttcgccg
      961 tgcccggcaa ccacgagtac ggactgagca agaacccgct gacgcacctc cccgagccga
     1021 tcccctggga gagttgcggc gtcagagagc tccgagactc ctgcgtgatg atcgatgtcc
     1081 ccgccttgcg gagcgccgag gtcgatccga gcatcgcggc cgctccgggc gcgtccggcg
     1141 gtcccgctgc aaggtccgcc agcggtccgg ccgccacggc cgacgaatcc gggggcgatc
     1201 ccgccgccac ggccgatggt cgccccgccg ctggcgcggc ccgcgtcgcg ctctgcggtg
     1261 ccgactactt gaccggaggg gcgccgctcg cgcatcagtc gatgccgctg gaggccgatc
     1321 tccgcctcct tctggtccac cgccccccca cggcggacga cccgctcgcc acgatgttcg
     1381 acctcgcctt ctcgggccac acccacggtg ggcagatccg gatcccgacg ccttgggggc
     1441 tccgcagccc gcacaacgac cacgtgtctc acttggaagg ggtccgcgct tggggcaacg
     1501 gcctgctggt cgtctcggcg ggcatcggca ccaccttcct acctctccgc ttgttcactc
     1561 ggccccaggt ggtgatccac cggctgactt ccgcccccag ccatgcagcc gaggcgcgtg
     1621 atgccttcca actccgaagg gaagccgaca cgggagagag cccttctggt atatatgagg
     1681 atgtcggcat ggcccacgac ggtggaggtg ctacgaatgc cagcggatga acaaccagcg
     1741 ggaggcgtga tcgtcccccc cgatgatcca gccgaagaga atcgcgccca cgtatccgag
     1801 gacgtagtcg ccacgtacgt ggctgcggcc gtgcgaagca tcccgggagt gagcgaactt
     1861 cacggcaagc cttggcagga gctgtccgaa cgcgtgcaca tgaacaccgc gcggaagggc
     1921 gtcgtgatca aggaggtggc gccgggcgtc gtggaggtgg aagtccacgt gtcggtggcg
     1981 tggggcaccg tcattcccga gatggcacag cggcttcagg agagcgtcgc gcacagcgtc
     2041 gagacccttc tcgacctcca ggtccgcaag accaccgtat acgtggacga ggtcgaggct
     2101 cccctcgagc tccgctaggg ctgtggccgc ctacgtcgaa gtcaccaccg cgacacaagg
     2161 cctcctgccg cccccctgct cggattgcat gtggtggcag acgacccagg ggcccgcgga
     2221 aggcagggag cgcaggctgg agtggatgcg cctcttggag acgagttggg gcagcgtggg
     2281 acttctggcc gtggaggatc gggagacggt cgcctctatc cagttcgccc ccgtccgctc
     2341 tctggcccgc ccccattctc ggcccttcgg ccgtcctccg gaggaagcgg tcctgctctt
     2401 ctgcctgcga ggccgggtag gcaggccgac cgtcgagacc caggaactgc ttcacagagc
     2461 catgtccgct ttgcggcggc gcggcgtgcg cgaggtctac ggatatgccc ggcctctgga
     2521 cactcacacc ctgagcggcg tgcgcaacct ctttggcctc gagttcctcg aggccaacgg
     2581 cttcgtagtg gcgcgcgcga tcgaccacgg ctacctcatg cgcgccgact tgggggggtt
     2641 gatccccgcg ctttccgagg ccggcggatc gatgcgccga cgcctcgctg gagccggaca
     2701 ccccagtccg gctacgttct ggcgcccctg acgctcctcc gggagagcca cggctgggac
     2761 ccacggtcag agcccacggt tagaaccgga aacccctggg atacaatgca ccgacgatga
     2821 agcgtccagc cccgcacctc gccgtcctca cgctcgcgct ggccgtcgcc ctcctgctga
     2881 cggccggcgt ggccgacttc gggccccggg tcgccttcgc cgcggaccaa ggtcgcgccc
     2941 ccgtgtgggt gacccgcgtg gagggggccg tcgtgccgcc tctgtccgcg tatctcgtgc
     3001 gcac
//
LOCUS       DASRMJ010000229         4739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_99269, whole genome shotgun sequence.
ACCESSION   DASRMJ010000229 DASRMJ010000000
VERSION     DASRMJ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99269"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFD74_06470"
     CDS             <1..206
                     /locus_tag="VFD74_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49226.1"
                     /translation="VHSELVDSYEPAGPWGVKEVGEGATLPTMGCFRNAIFDAMDVSV
                     NSLPLGFEKVWRALKDKREAGDQ"
     gene            546..2144
                     /locus_tag="VFD74_06475"
     CDS             546..2144
                     /locus_tag="VFD74_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZK49227.1"
                     /translation="MLQKTADRLPEKIALVDGDTRLTYGQVLEEASRLSSALSKLGLT
                     KGSVAAVYLPNAAELVTLFFALQKLGVVIAWVNPNYREHEVRFILEDSGAETLFLFEE
                     WAGYGCLSAVLSLDGLPQLRTIVVARPTDGFIADDERVTTSADVVAMADRDPAAASSL
                     PGAAVSPDDLSMLIYTSGTTARPKGAMIRQSQTVRAGFSYSLGVNATEDDVFIGFLPM
                     SHSYGCGALLVQPFILGARLVMLDRFSPERAFALIEKERVTIQLAAPAHYILELDSST
                     RADFDLSSVRAGLIAGQIAPAGLITKVEREMGVHIASFLGSSEVGPGLSIILPYPSPL
                     EVRERSIGYPLIGTEASIADPATGQELPAGESGELILRGWHVTQGYWNNPDETANQIR
                     DGWLHTGDLAMRDEDGCFSILGRIKDCINRGGFKVIPSEIEAILVQQPHVSEACVVGT
                     PNPVLGESICACVILDASADGLTLSDVRTHMKGRVADFKLPDEMVILTELPRMPGGVK
                     VNRYGTGGVVELARESTDKQTLRR"
     gene            2267..3391
                     /locus_tag="VFD74_06480"
     CDS             2267..3391
                     /locus_tag="VFD74_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZK49228.1"
                     /translation="MRRSMFMLVGVALLLSFTVLVGCGGGTTTTTAAEATTTTAAEAT
                     TTTAETLDDTVYELSIAHIWPAGHAIQTRLIDPMAQEISDATGGHVKLVSYPGGTLLK
                     GPEVYEGVKTGVADMGISVYGYNAGRFPIVEAFLLPGISWATAKSSGAALTEGLATLD
                     AAEIKDTHVLMSFATGPGEMLMKVPVRSLADLKGLELGATAGPRGDGLKLLGATPVVL
                     PMPEVYEAQSRGVIKGVIGPYEAMTGFKLADVTDYITDTPFLYVNYFFLTMNNDKFDS
                     LPAPYQEAITTITQKYYNDVTLGLFDDLNKEALDAALASKDIEMITLSDAEQTLWKEK
                     MAPIFADYQAVLDGKGLDGAKIIETVRALSDKYNAMYPAQ"
     gene            3482..4030
                     /locus_tag="VFD74_06485"
     CDS             3482..4030
                     /locus_tag="VFD74_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZK49229.1"
                     /translation="MRILQRLTTAFDWVAGWVVLASMLLVAGNVVLRPFDRPIGGTYE
                     WTGFVTALAIGLALAHCAIQGGHTIINLFVDRLPTRVQQAVRMFARLLTGTFLSVATW
                     RVTVYAISTWKSGEVAPTTKVPFYPIMFVVALGIVLYALVEFSKAVRFVTGWLEPRAV
                     MDYAPGPDSPSMPADGWEEESL"
     gene            4027..>4739
                     /locus_tag="VFD74_06490"
     CDS             4027..>4739
                     /locus_tag="VFD74_06490"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZK49230.1"
                     /translation="MSPTLIGVISVVVLLGLMALEVPIAVAMAAVGFVGFAVVVSPEA
                     AYNVVAKDIFNTFNSYTMSMLVMFVLMGYFAYHAGIGAQLYQFANKTMGYVPGGVAMA
                     NEVACALFGAVCGSSTATAATMGSIALPEMRKLKYADSLSTASVAAGGTLAILIPPSV
                     IFVLYGLATEQSIGTLFLAGILPGIMLTVLYMLAILITVLRHPELAPRQEGRPHFKEV
                     IHTLKGGLIETLLIFALSLG"
BASE COUNT          855 a         1575 c         1456 g          853 t
ORIGIN      
        1 gggtccactc ggagttggtt gattcctacg agcccgccgg gccgtggggc gtcaaggaag
       61 tgggtgaagg cgcgaccctt cccaccatgg gctgcttccg caacgccatc ttcgacgcga
      121 tggacgttag cgtcaacagc ctgccgctgg gcttcgagaa ggtgtggcgc gctctgaagg
      181 acaagcgcga agcgggcgac cagtagtgac tcagcgagcg gccgccgcgg agggttcctc
      241 cccggcggcc gcggcttacg cgcctcggtc ggcggcgacc ccacactgac cgccgctgac
      301 tccgacgacc gttgcagtgc acaagagcgc cgctccacag aacaatcgag caacttatat
      361 atataggtag gtaacttctt gaccggcgct caggcgactg ctatggtggc caagactgaa
      421 gggagaaccg ctgcggcggc ttcaggccgt tccggctggt agcagtccga ggcattgggg
      481 gtagtccgat agacagcacg ctggctggat cctacgagca gctgagcggt tcgacggtgt
      541 ggcaggtgct tcagaagacc gccgaccgac ttcccgaaaa gatcgcactg gtcgacggcg
      601 acacccgtct cacgtacggc caggtgctcg aagaggcgtc ccgcctctcg agcgcgctct
      661 ccaaactggg gctcaccaaa ggcagcgtgg cggccgtcta cctgccgaac gcagccgagc
      721 tcgtcaccct cttcttcgcg ctgcagaagc tgggcgtcgt catcgcatgg gtcaatccca
      781 actaccgcga gcatgaagtc cgcttcatac tcgaagactc cggcgctgag actctcttcc
      841 tcttcgagga gtgggctggc tacggctgcc tctctgccgt cctctctctc gacgggttgc
      901 cgcagctgcg cacgatcgtc gtggcgcggc cgacggacgg cttcatcgcc gacgacgagc
      961 gggtcacgac ctctgccgat gtggtcgcga tggcagaccg ggacccggcg gcggcctcca
     1021 gccttccggg ggcagcggtc tcgcccgacg acctgtccat gctgatctac acttccggca
     1081 ccacggcccg tccgaaaggg gccatgatcc gtcagtcgca gacggtgagg gccggcttct
     1141 cctactcgct cggagtgaac gccaccgagg acgacgtgtt catcgggttc ctccccatga
     1201 gccactcata cgggtgtggc gccttgctgg tgcaaccctt catcctgggc gccaggttgg
     1261 tgatgctcga ccgcttctct cccgaacggg cctttgcgct catcgagaag gagcgcgtca
     1321 cgatccagct ggcagctccg gcccactaca tcctcgagct cgacagctcg acgcgcgccg
     1381 acttcgacct ttccagcgtc agagccggcc tcatcgcggg ccagatcgct cccgcaggct
     1441 tgatcacgaa ggtggagcgg gagatgggcg tccacatcgc ttccttcttg ggatcgtcgg
     1501 aggtggggcc cgggctctcc ataattcttc cctatccttc gcctctggag gtgagagagc
     1561 gttcgatcgg ctatcccctc atcggcaccg aggccagcat cgccgaccct gccacagggc
     1621 aagagctgcc ggccggagag tcgggagagc tgatcctccg tggctggcac gtcacgcaag
     1681 gctactggaa caaccctgac gagacggcca accagatacg cgacggctgg ctgcatacgg
     1741 gcgacctggc catgcgggac gaagacggct gcttcagcat cctgggacgc atcaaggact
     1801 gcatcaaccg cggaggcttc aaggtcatcc cttcggagat cgaagccatc ctggtccagc
     1861 agccgcacgt gtctgaggcg tgcgtggtcg ggactccgaa cccggtcctg ggagagtcga
     1921 tctgtgcgtg cgtgatcctc gacgcgagtg ccgacggcct cacgctctcc gacgtcagga
     1981 cccacatgaa gggccgcgta gccgacttca aactgcccga cgagatggtc atactcaccg
     2041 aactcccacg catgccggga ggagtcaagg tgaaccgata cggaacgggc ggcgtggtcg
     2101 agttggcaag agagtccacg gacaagcaga ctctgaggcg ttgacgacca ctcgcttgta
     2161 caccactcgg gggtgtgtat gaaatgatcc gtgtagagta gtcgcaggag cggttcaagg
     2221 aatccgggcc gcgtggccca caccaagggg gagagcagtg ataaagatgc gtaggtccat
     2281 gttcatgctc gttggagtag ctctgcttct gtcgttcacc gtactcgtcg gttgcggcgg
     2341 cggcacgacc accacgaccg ccgccgaagc caccacgacc acggcagccg aagccaccac
     2401 gacaaccgcc gagacactgg acgacaccgt ctacgaactc agcatcgcgc acatctggcc
     2461 ggccggccac gcgatccaga cccgactcat cgacccgatg gcccaagaga tcagcgacgc
     2521 caccggcggc cacgtcaagc tcgtcagcta tcccggcggc accctcctga agggccctga
     2581 agtctacgag ggcgtcaaga ccggcgttgc cgacatgggt atctcggtgt acggctacaa
     2641 cgccggccgc ttcccgatcg tcgaagcgtt cttgcttccg ggcatcagct gggcgaccgc
     2701 caagtcgtcg ggcgcggcgc tgaccgaagg tcttgcgacc ttggacgcgg ccgagatcaa
     2761 ggacacgcac gtgctcatga gcttcgccac cggccccggt gagatgctca tgaaggtgcc
     2821 ggtgcgcagc ctcgccgacc tgaagggtct cgagctcggc gccaccgccg gtcctcgggg
     2881 cgacgggctc aaactgctcg gcgcgacccc ggtggtcctc ccgatgcctg aggtctacga
     2941 agctcaatcg cgcggcgtca tcaagggcgt catcggcccg tacgaagcca tgaccggctt
     3001 caagctcgcc gacgtgaccg actacatcac cgacacgccc ttcctgtacg tgaactactt
     3061 cttcctgacc atgaacaacg acaagttcga ctcgttgcct gctccgtacc aggaagccat
     3121 cacgaccatc actcagaagt actacaacga cgtcaccctc ggcctgttcg acgacctcaa
     3181 caaggaagct ctcgacgcgg ctttggcctc gaaggacatc gagatgatca ccttgagcga
     3241 cgccgagcag actctgtgga aagagaagat ggccccgatc ttcgccgact accaagccgt
     3301 cctcgacggc aagggactcg acggtgccaa gatcatcgag accgtcagag cgctgtcgga
     3361 caagtacaac gcgatgtatc ccgctcagta acgatcgact gtcgaggcgg ccccggcacg
     3421 ccggggccgc ctcgatcttc tcgcgcccag accaccgacc cagcgacccg ggggcacgca
     3481 catgcggata ctgcagagac tgacgaccgc gttcgactgg gtcgcggggt gggtcgtact
     3541 ggcctcgatg ctgcttgtgg ccggcaatgt cgtgcttaga ccgttcgacc ggcccatcgg
     3601 gggcacctac gagtggaccg gcttcgtgac agccttggcc atcggactgg ccttggctca
     3661 ctgtgccatc cagggtggcc acaccatcat caacctgttc gtcgaccgtc tgcccaccag
     3721 ggtgcagcag gcggtgcgca tgttcgcacg gcttctgacc ggaacctttc tttccgtcgc
     3781 aacctggcgc gtcaccgtgt acgcgatcag cacgtggaag agtggagagg tggcgcctac
     3841 gaccaaggtg ccgttctatc cgatcatgtt cgtggtggcc ctcggcattg tcctctacgc
     3901 gctggtggag ttctctaagg cggtgcggtt cgtcacgggc tggctcgagc cgcgtgccgt
     3961 catggactac gctccgggtc cggactctcc ctcgatgccc gctgacgggt gggaggaaga
     4021 gtccctatga gccccacact catcggagtc atcagcgtcg tcgttcttct cggcctgatg
     4081 gcgctcgaag tgcccatcgc cgtggcgatg gcggccgtcg gcttcgtggg cttcgcggtc
     4141 gtcgtgtcgc ccgaggcggc ctacaacgtt gtcgccaagg acatcttcaa caccttcaac
     4201 tcgtacacta tgagcatgct ggtgatgttc gtgttgatgg gctacttcgc ctatcacgcc
     4261 ggcatcggag cccagctcta ccagttcgcg aacaagacca tgggttacgt gcccggtgga
     4321 gtcgctatgg ccaacgaagt ggcctgcgct ctgttcggcg ccgtgtgcgg ctccagcacc
     4381 gccaccgccg ccaccatggg cagcatcgct ctgcccgaga tgcgcaagct caagtacgcc
     4441 gactcgctct ccaccgcgag tgtcgcagcc ggcggcacgc tggccatcct cataccgccc
     4501 agtgtcatct tcgtcctcta tgggttggcg acggagcagt cgatcggcac gctgttcctc
     4561 gccggcatcc tcccggggat catgctcacc gtcctctaca tgctggccat cctgatcacg
     4621 gtgctgaggc atcccgagtt ggccccgcga caagagggtc gacctcattt caaggaggtc
     4681 atccacacgc tcaagggcgg cctcatcgag acccttctca tcttcgccct ctccttggg
//
LOCUS       DASRMJ010000230         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_271022, whole genome shotgun sequence.
ACCESSION   DASRMJ010000230 DASRMJ010000000
VERSION     DASRMJ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271022"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3163
                     /gene="ileS"
                     /locus_tag="VFD74_06495"
     CDS             <1..>3163
                     /gene="ileS"
                     /locus_tag="VFD74_06495"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZK49231.1"
                     /translation="QTRERTGGGCAHEVCRHDADHVFRPVDTAQRFPALEESILGWWR
                     ENRIFEKSVDLRRGQPEWVFYEGPPTANGRPGSHHVLARVFKDIYPRFRTMRGNFVPR
                     KAGWDCHGLPVELEIEKRLGFNHKTEIEEYGIAEFNRLCRESVTQYVDEWKRMTERIG
                     FWIDLDDAYWTMNNSYIESEWWILTEFWKQGLLEQDYKVVPYCPRCGTALSSHELAQG
                     YKEVTDPSIIVRFPLLDAEGRLEDGTSLLVWTTTPWTLISNVAAAVGEKIVYARARLG
                     DEAFILGADLVEKVLGPEARVEETFPGSALAGRRYRPPFDFVTPDKPAWRVVTGDFVS
                     TDEGTGIVHIAPAFGADDMVVGRANDLPVIMPVDADVKFTEDVPPYAGVFVKDADKAI
                     IRDLDDAGILFSSRPHTHSYPHCWRCDTALIYYAKASWYVRTTQRKDDLLAANEAVNW
                     YPEHIKHGRFGDWLENNIDWSLSRERYWGTPLPIWRCPEGHDHCVSSRAELSDLAGRD
                     LSDLELHRPYVDDIIFPCPTCGAEAHRVPEVIDAWFDSGSMPVAQWHYPFENKELWEQ
                     RFPADFICEAIDQTRGWFYSLIAISTLLTKTSSYKNVVCLGHILDGEGRKMSKSLGNV
                     VDPWTVLDTQGADALRWYLFTVSSPWFARRFSPDHIDEVLRKFTLTLWNTYSFFTLYS
                     NIDGFDPRAHAMPVRDRTLMDRWIVSELHQLARTVTDGLEVYDAFSTGRAIADFVDEL
                     SNWYVRRSRRRFWKSEEDADKVAAYLTLYECLEMLSKLLAPYMPFMAEELHRNLVGER
                     YADAALSVHLSDWPAADETLIDEDLSFRMGVARRAVNMGRAARNASQLKTRQPLASAV
                     VAAGEREWAALVSLSDVVCEELNVKVLESAASAGELVNYKVKPNYRTLGPRFGKAMPL
                     VAAAVDALDGGEVETALRSGEPVSVSVAGESHQLTQEDLLVEVRQREGYVVEREGDLV
                     VALSTELTPALLTEGLARELVHHVQNTRKAAGLQIEDRIHLAVAGPREILDMLEESSE
                     WVKKETLSVSLEASAVVSGVMGAE"
BASE COUNT          559 a         1043 c         1033 g          528 t
ORIGIN      
        1 cagacgagag aacggacggg cggcggctgc gcccacgagg tgtgccgcca cgacgccgac
       61 cacgtgttcc gtcccgtgga cacagcgcaa cgcttcccgg cgctcgagga gtccatcctc
      121 ggatggtggc gggagaaccg catcttcgag aagagcgttg acctgcgtcg gggacaaccg
      181 gagtgggtct tctacgaggg ccccccgacc gccaacggca ggcccggctc gcaccacgtg
      241 ctcgcacgcg tcttcaagga catctacccc cggttccgca ccatgcgcgg caacttcgtg
      301 ccacgcaaag cagggtggga ctgtcacggc ctgcccgtgg aactcgagat cgagaagcgc
      361 ctcggcttca accacaagac cgagatcgag gagtatggca tcgccgagtt caaccggctg
      421 tgcagagagt cggtcacgca atacgtcgac gagtggaagc gcatgaccga gcgcatcggt
      481 ttctggatcg acctcgacga cgcctactgg accatgaaca acagctacat cgagtcggag
      541 tggtggatcc tcaccgagtt ctggaagcag ggactgctcg aacaggacta caaggtcgtc
      601 ccctactgcc cgcgttgcgg caccgcgctc tcctcgcacg aactcgcgca ggggtacaag
      661 gaagtgaccg acccctcgat catcgtgcgc ttcccgttgc tcgacgccga ggggcgcctc
      721 gaggacggca ccagcctgct ggtgtggacg accacgccct ggaccctgat ctccaacgtg
      781 gcggccgccg tgggcgagaa gatcgtgtac gcgcgggcac gtcttggcga tgaggccttc
      841 atcctcggcg ccgacctggt ggagaaggtg ctcggccccg aggcccgggt ggaggagacc
      901 ttcccggggt ccgcactggc cggaaggcgc taccgtccgc ccttcgactt cgtcacgccc
      961 gacaaaccgg cttggcgggt cgtcaccggc gatttcgtga gcaccgacga gggcaccggc
     1021 atcgtgcaca tcgccccggc cttcggcgcc gacgacatgg tggtggggcg cgcgaacgac
     1081 ctgccggtga tcatgccggt ggacgcggat gtcaagttca ccgaggatgt gccgccctac
     1141 gcgggcgtgt tcgtcaagga cgccgacaag gccatcatcc gcgatctgga cgacgccggc
     1201 atcctcttct ccagccgccc gcatacgcac agctatccgc actgttggcg ctgcgacacc
     1261 gcgctcatct actacgccaa ggcttcctgg tacgtgcgca ccacgcagcg aaaggacgac
     1321 ctgctcgccg ccaacgaggc ggtgaactgg tatccggagc acatcaagca cggccgtttc
     1381 ggcgactggc tcgagaacaa catcgactgg tcactctcgc gggagcgcta ctggggcacg
     1441 ccactgccca tctggcggtg cccggagggg cacgaccact gcgtctcgtc gcgggcggag
     1501 ttgtccgacc tcgccgggcg cgacctctcc gatctcgaac tgcatcggcc ctacgtggac
     1561 gacatcatct tcccctgccc cacgtgcggc gcggaggccc accgcgtgcc cgaggtcatc
     1621 gacgcgtggt tcgactccgg ctccatgccg gtggcccaat ggcactatcc ttttgagaac
     1681 aaggagctct gggagcagcg tttcccggcc gacttcatct gcgaggcgat cgaccagacg
     1741 cggggctggt tctacagtct catcgccatc agcacgttgc tgaccaagac gtcgagctac
     1801 aagaacgtcg tgtgcttggg ccacatcctc gacggcgaag gccgcaagat gtccaagagt
     1861 ctgggcaacg tggtggatcc ctggacggtg ctcgacactc aaggggccga cgccctgcgg
     1921 tggtatctct tcaccgtgag ctcgccctgg ttcgcgcggc gcttcagccc cgaccatatc
     1981 gacgaggtgc tgcgcaagtt caccctcacg ctctggaaca catactcatt cttcacgctc
     2041 tactcgaaca tcgacggctt cgacccccgc gcccacgcga tgccggtgcg agatcgcact
     2101 ctcatggacc gttggatcgt ctccgagctg caccaactgg cacgcacggt gacggacggg
     2161 ctcgaggtgt acgacgcctt ctcgaccggc cgcgcgatcg ccgacttcgt cgacgagctc
     2221 tcgaactggt acgtgcgtcg ctcgcgccgt cgtttttgga agagcgagga ggacgccgac
     2281 aaggtggccg cctacctcac gctctacgag tgcctggaga tgctctcgaa gctgctcgct
     2341 ccctacatgc cgttcatggc cgaggagctc caccgcaacc tggtgggtga acgctacgcg
     2401 gacgccgcgt tgtcggtgca tctgagcgat tggcctgcgg cggacgagac tctcatcgac
     2461 gaggacctgt cgttccgcat gggggtggcc cggcgtgccg tcaacatggg cagggccgcc
     2521 cgcaacgcgt cgcagctcaa gacgcgccaa cccctggcca gcgcggtcgt ggccgccgga
     2581 gaacgggagt gggcggcgct ggtgagtctc tccgatgtgg tgtgcgagga gttgaacgtc
     2641 aaggtgctcg agtcggcggc atcggccggt gaactcgtca actacaaggt gaagcccaat
     2701 tacaggacac tgggcccgcg cttcggcaag gccatgccgc tggtggccgc cgccgtcgat
     2761 gcgttggacg gcggcgaggt cgagaccgcg ctgcgatcag gtgagcccgt ctctgtttcg
     2821 gtggccggcg agtctcacca gttgacccag gaagaccttc tggtcgaggt gcggcaacgc
     2881 gaaggctacg tcgtggaacg ggagggcgac ctggtggtgg ccctctctac cgagctgact
     2941 cctgcgttgc tcaccgaagg actggcgcgg gaactagtgc accacgtgca gaacacccgc
     3001 aaagcggcgg gcctgcagat cgaagaccgt atccacctgg ccgtcgccgg gccccgtgag
     3061 atacttgaca tgctggagga atcgagcgag tgggtcaaga aggagaccct cagcgtgtcg
     3121 ctcgaggcgt ccgcggtcgt gtcgggcgtc atgggcgctg aag
//
LOCUS       DASRMJ010000231         4323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_307532, whole genome shotgun sequence.
ACCESSION   DASRMJ010000231 DASRMJ010000000
VERSION     DASRMJ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307532"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFD74_06500"
     CDS             complement(<1..290)
                     /locus_tag="VFD74_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004163212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZK49232.1"
                     /translation="MERDNALRVLTAHREELSALGVKEISIFGSVARDEARIGSDVDV
                     LVEHQVGAPLSFFRVCRLRYFLEKILGERVDLVTLGGLRPEIRDEVMAEAISA"
     gene            422..518
                     /locus_tag="VFD74_06505"
     tRNA            422..518
                     /locus_tag="VFD74_06505"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:456..458,aa:Sec,seq:tca)
     gene            779..1252
                     /locus_tag="VFD74_06510"
     CDS             779..1252
                     /locus_tag="VFD74_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49233.1"
                     /translation="MNGPPSVLVYEIDTAPREVLRTLDTESSWSKVVEVGADGTKIYV
                     ANWVGDDVSEIDLSTGECTRRFRTGDTPRGLYATEDGAYLYVATYDDGELQRIDLATG
                     KVVTLFDNNGALRYIVADAERGQLYISDMRNDAVFVHDMASGETREFAATDEKMR"
     gene            1300..1422
                     /locus_tag="VFD74_06515"
     CDS             1300..1422
                     /locus_tag="VFD74_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49234.1"
                     /translation="MLVFSDFLDDRLRLYEVPDTAALRAGGGGRWEQHFEELAK"
     gene            1667..3235
                     /locus_tag="VFD74_06520"
     CDS             1667..3235
                     /locus_tag="VFD74_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK49235.1"
                     /translation="MKKYGLLLVFMLLLGLGAALLLTGCTSGGTTASTGGDAAANTFT
                     YAQGADPRGLDPALVDDGESSKVISNIYEGLLKYAPDSTKVEPCLAESWEVSADGLSY
                     TFKLKQGVKFQDGTDFNADAVKFNIDRQIPPNATADMSYGPFVYGSVKDVVVNDPSSV
                     TINLTQTNTAFLANLAMSLAAPIVSPKALTDGNNSVMENPIGTGPYKFVKWNKGENVV
                     LVRNDDYWGEKAKIENVVFRIIPDNSARVLALNNGEVDMIDGIDATVVDKITEGGNKL
                     FEAAGMNVNYMAYNTTSDLLKTVEARTAVSQAINVEELVQSLYQGYSEKATTILPTFM
                     PGYDSTVQQVDYDLEAAKAGLAKAGITKLHMIAYSNPRPYNGATGAALAAAIQGYLQK
                     AGVESTIDTFDWATYKEKVATGDYDICFYGWIGDNGDPDNFLNLLADKDPTMNVARWQ
                     DPTYIAGIKAALSMPNGADRDAAYGALEKYAAENAVWLPLSHAKTLAGYRPNVSGFIY
                     HVTGNVFLSQAEKK"
     gene            3326..>4323
                     /locus_tag="VFD74_06525"
     CDS             3326..>4323
                     /locus_tag="VFD74_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49236.1"
                     /translation="MLEYVLKRIAMVVPVMIGVSIIVFALMRVFSPDPAPIVLGQHAT
                     QEAMVAWREEKGLNEPVVAQYWSYVRGAFHGDLGTSYYSSTPVAREIGARFPATIELA
                     VAAIIFASTFGILLGVVAAVKKNSIFDAAGMLIALVGVSIPIFWLGIMLIMLFSGYLH
                     LLPTGGRINVLLEPAEVTGFYMIDSLLEGNWAAFKDAFIHLLLPALTLGMYSMAIITR
                     MTRSAMLDTLSQDYVRTSRAKGISELKVIGKHALRNSLIPVTTVIGLQFGALLGGALL
                     TESVFAWPGIGKYTVDSILKSDFPMVQGVVLLVAVVFVTVNLVADLMYAYLDPRIK"
BASE COUNT          784 a         1404 c         1339 g          796 t
ORIGIN      
        1 gcactgatgg cctcggccat gacctcgtcc cgtatctccg gcctcagacc acccagcgtg
       61 accagatcga ccctttcgcc gaggattttc tccaggaagt agcgcagccg acaaacccgg
      121 aagaagctga gcggagcacc gacttggtgc tctaccagca catcgacgtc gcttccgatc
      181 cgggcttcgt cccgcgccac ggagccgaag atcgaaatct ccttgacgcc aagagcagaa
      241 agttcttcgc ggtgggccgt cagtactcgt agggcgttgt ctcgttccat ttgggtcgat
      301 cacctcccat ctcgggccat tgtcgcattt cgagcagtga gtcgccatgt gtggtgacgg
      361 caagcgcgag tcttgccggt tccgctaccc gggcggatgc gctagaatcg tggtccttcg
      421 cggagtggca tccggtctgg tggccggccc ggtcttcaaa accgctgttc ggcggtgatc
      481 ccgtcgaagg taggttcgat tcctacgccg ctccgccaat ccactttcag gggtctgtcc
      541 agattgaacc cgggttcggc gagtcggtgc ccatttcccg cgttcacagc ccgtccggat
      601 ggcgcggtag aatgattcct gttgaccgat cgagccgatc agtcggaagc gggcggccgg
      661 gcttgagtca gatggagggt cgcgagccgt accgggggta cgcttccgct tcgggctccc
      721 cgcgcgatgg cccccccggg ggcgcgcgcg cgagggctcc cagatctgga ccacgatctt
      781 gaacggtccg ccgagcgtgc tcgtctacga gatcgacacc gccccccgcg aggtcctgcg
      841 gacgctcgac accgagagct cctggagtaa ggtggtcgag gtcggcgccg acggcaccaa
      901 gatctacgtg gcgaactggg tgggcgacga cgtctccgag atcgatctct ccaccggcga
      961 atgcacccgc cgtttccgta cgggcgacac tccgcgcggt ctttatgcca ccgaagatgg
     1021 cgcctatctc tacgtggcca cctacgacga cggcgagctg cagcgcatcg acctggcgac
     1081 cggcaaggtg gtcaccctct tcgacaacaa cggtgccctg cggtacatcg tggccgatgc
     1141 ggagcgtggg cagctctaca tctcggacat gcgcaacgac gccgtgttcg tgcacgacat
     1201 ggcgagcggc gagacgcgcg agttcgcagc cacggacgag aagatgcgat agtcggcggc
     1261 aaccagtgca ccgctctcgc gctctccgac gatggatctt tgctcgtgtt ctcggacttc
     1321 ctcgacgacc gcctccggct ctacgaagtg cccgacaccg cagcgctgcg cgcgggcggc
     1381 ggcgggcgct gggagcagca ctttgaggag ctcgcgaaat gattcggcgg ccgaggcacc
     1441 gaggaatgcc tcttccacct ccaagcactc agggccggct acacgcaaaa agagaccggc
     1501 cggcctaggt tggtcgtcgc cgtatttcat gtcctgtcgg gactcggcag cgacatgatt
     1561 tccctgattt ctgccggagg agtacactgt gctggtcgcg gttttcgaca ggtgtttctg
     1621 ctgccagatg ggccgtcgtg aggactcggg gaataagggg tgatacatga agaagtacgg
     1681 tttgttgctg gtgttcatgc tcctgttggg cctcggagcg gcgctgctgc tgacggggtg
     1741 cacctcgggc ggcaccacgg cctccaccgg cggcgatgcg gctgccaaca ccttcaccta
     1801 cgcgcaggga gccgaccctc gtggtctgga cccggcgctc gtggatgatg gtgagtcgtc
     1861 gaaggtcatc tcgaacatct acgaaggtct tctgaagtac gcgcccgatt cgaccaaggt
     1921 cgagccctgt ctcgctgagt cttgggaggt cagcgcggac ggcctgagct acaccttcaa
     1981 actcaagcag ggagtcaagt tccaggacgg caccgacttc aacgccgatg ccgtgaagtt
     2041 caacatcgac cgtcagatcc cgcccaacgc gaccgccgac atgtcgtacg gtccgttcgt
     2101 gtacggctcg gtcaaggacg tcgtggtcaa cgatccctct agcgtcacta tcaacctcac
     2161 gcagacgaac accgcgttcc tcgcgaatct cgccatgagc ctggccgcac cgatcgtgag
     2221 tccgaaggcg ctcaccgacg ggaacaacag cgttatggag aaccccatcg gcactggtcc
     2281 gtacaagttc gttaagtgga acaagggcga gaacgtcgtg ctcgtccgca acgacgacta
     2341 ctggggtgag aaggcgaaga tcgagaacgt cgtctttcgc atcatccccg acaactccgc
     2401 tcgcgtcctc gcgctcaaca acggtgaggt cgacatgatc gacggaatcg acgcaacggt
     2461 cgtcgacaag atcacggagg gcggcaacaa gctcttcgag gcggctggga tgaacgtcaa
     2521 ctacatggcg tacaacacca cctcggacct cctgaaaacc gtggaggctc gcacggccgt
     2581 atcgcaggcc atcaacgtcg aggagctcgt gcagagcctc taccagggct actccgagaa
     2641 ggcgaccacc atcctgccga cgttcatgcc cgggtacgac tcgaccgtcc agcaggtgga
     2701 ctacgatctg gaagccgcaa aggcgggcct cgccaaggcg ggcatcacga agctccacat
     2761 gatcgcgtac tcgaacccgc ggccgtacaa cggagccacc ggcgcggcgc tcgcggccgc
     2821 catccagggc taccttcaga aggccggcgt ggagtccacg atcgacacct tcgactgggc
     2881 cacctacaag gagaaggtcg cgacaggcga ctacgacatc tgcttctacg gctggatcgg
     2941 tgacaacggc gacccggaca acttcctgaa tctgctcgcc gacaaggatc cgaccatgaa
     3001 cgtcgccagg tggcaggatc ccacctacat cgccggcatc aaggcggcgc tctctatgcc
     3061 gaacggtgcg gatcgcgacg ctgcgtacgg cgcgctggag aagtacgccg ccgagaacgc
     3121 ggtctggctg cccctgtcgc acgccaagac gctggccggc taccggccca acgtctccgg
     3181 gttcatctac cacgtgaccg gcaacgtctt cctgtcgcag gcggagaaga agtagccgct
     3241 ctcatactcc gcgtttggtc gcagccggaa gccacgacag tggcttccgg ctgcgttctt
     3301 cccttatgat cgagaggctc gcggcgtgct cgagtacgtg ctgaagagga tcgcgatggt
     3361 cgttccggtg atgatcggag tgtccatcat cgtcttcgcg ctgatgcggg tgttctcccc
     3421 cgacccggcg cccatcgtgc tgggccagca cgccacgcag gaggccatgg tcgcgtggag
     3481 ggaggagaaa ggcctgaacg agccggtggt cgcccagtac tggtcgtatg tcaggggcgc
     3541 cttccacgga gacctaggca cctcgtacta ctccagtacc ccggtagccc gcgagatcgg
     3601 cgcgcggttc ccggcgacga tcgagctggc cgtggcggcg atcatcttcg cttccacctt
     3661 cggcatcctg ctgggcgtcg tcgcggcggt gaagaagaac tccatcttcg acgcagccgg
     3721 catgctgatc gccctggtcg gcgtgtccat tcccatcttc tggctcggca tcatgctcat
     3781 catgctcttc agcggctatc tccacctgct gcccacgggg gggcgcatca acgtgctgct
     3841 cgagcccgcc gaggtgaccg gcttctacat gatcgactcg ctgctcgagg gcaactgggc
     3901 cgccttcaag gacgcgttca tccacctcct cctgccggcg ctcaccttgg ggatgtactc
     3961 gatggccatc atcacccgca tgacccgctc ggccatgctc gataccctca gccaagacta
     4021 tgtccgcacg tcgagggcca agggcatctc tgagttgaag gtcatcggca aacacgcgct
     4081 gcgcaactcg ttgatcccgg ttacgaccgt catcggcctg cagttcggcg cgctgctcgg
     4141 cggggcgctc ctcaccgaga gcgtgttcgc gtggcccggc atcggcaagt acacggtcga
     4201 ctccatcctg aagtcggact tccccatggt gcagggggtc gtgctcctcg tggcggtggt
     4261 cttcgtgacc gtcaacctgg tcgccgacct gatgtatgcg tacctcgacc cgcgtatcaa
     4321 gta
//
LOCUS       DASRMJ010000232         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_138413, whole genome shotgun sequence.
ACCESSION   DASRMJ010000232 DASRMJ010000000
VERSION     DASRMJ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138413"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2713)
                     /gene="infB"
                     /locus_tag="VFD74_06530"
     CDS             complement(<1..2713)
                     /gene="infB"
                     /locus_tag="VFD74_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZK49237.1"
                     /translation="MAKKRIYVLAKERGLGTPELAERLRAAGIEVKSNVSSVEESDVE
                     RVLGPLAAEEPAAESTSSEAEVTAEDQTPEQTEASESAAAPDDAIGPEDEVQTAGTLG
                     LTDLPVAAESPAAEKAAEATPPVKPAAKEAGVRKSSTRPAPGPRSAPKPDGPLGPKIV
                     ATAEEVRAAQEAAAARVAQAAPGQRRGGKGRGSRLEGIPTVVGPGVPMRKGVNASGTV
                     VRKGEEPPAPPAPKPEEKPAAKAPQAGGRPPAAPRATAPRPAAAGAPPTTRKPAKTDA
                     PAVAVDAKGASRRKAGGESTYTPGGRMWKPGETASRRRVVIDSQAGRRGGRGVGKGVR
                     GPVGLTESERAPIKKKAAPGSETPVKIASGAMVKDLADALELGPGEIIKTLMKLGELV
                     TITQSLSDDAIMIVAEEFERKVEVQHAEEEGEVTFEDDPADLVERAPVVTVMGHVDHG
                     KTSLLDAIRETEVVKGEAGGITQHIGAYQAHHGDRRVTFIDTPGHEAFTAMRARGAKV
                     TDVAVIVVAANDGVMPQTIEAIDHARAADVPMLVAINKMDLGDANPDRVKQQLSDHGL
                     QPEEWGGDTVMVEVSAKKRLHLEELMNMILLVADVQELKANPDAPASGVVVESQLDVG
                     RGPVATVLVQRGTLHVGDAIVCGESYGKVKAMLDFRGDVITAAGPSVPAQVLGFNSVP
                     AAGDQALAVKDEREARQMAEGRTARLRMEMNAKGVAGATSLDDFYRRLKEGEVKELAL
                     VLKGDVGGSVEALEEALNNITHPEVKVRVIHSGVGGINESDVNLAAASNAVIIGFNVR
                     PSVSAKASADEQGVDIRTYRVIYKAIEDIEAALMGMLEPEHVEQTLGAVEVRQIFRAS
                     RIGTIAGCQVLSGKITRNAKVRVVRDGSIVFEGGLGSLKRFNEDVREV"
     gene            complement(3041..>4128)
                     /gene="nusA"
                     /locus_tag="VFD74_06535"
     CDS             complement(3041..>4128)
                     /gene="nusA"
                     /locus_tag="VFD74_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZK49238.1"
                     /translation="NKTANAVPFSDVEIDQDTGEIHVFQLTFPPGLEPDLDVEEGEEV
                     EIDYSIADREEIIPDDFGRIAAQTAKQVIYQRIREAEQDLFFKEYSTRVGEIVTGIVQ
                     QSDNRYTLVDLGRVEGLLPKSEQVPTERYEHGMRLKAVIKDVEQNPKGPPIILSRRSD
                     ELIRRLFELEVPEIADGLVEIVGVAREPGYRSKISVVSHSDGVDPVGACVGPRGSRVR
                     MVVSELRGEKIDIIPFNEEPARFVAKALSPARVREVFLDDQTREATVVVPDDQLALAI
                     GRDGQNARLANRLTGWKVDIKSETEMSQEEQSFSVTEQDEVTMDGRCRALTGAGKRCP
                     NAALPDSSYCGIAAHQALAAEGDDEEV"
BASE COUNT          618 a         1467 c         1258 g          785 t
ORIGIN      
        1 ggacctcccg cacgtcttca ttgaagcgtt tgagagaccc caagccacct tcgaagacga
       61 tgctcccgtc acggaccacc cgcaccttgg cgttcctggt gatcttcccg gagagaacct
      121 ggcagcccgc gatggtgccg atgcgcgacg cacggaagat ctggcgcact tcgacggcac
      181 cgagagtctg ctccacgtgc tcaggctcca acatccccat caacgcggct tcgatgtcct
      241 cgatggcctt gtagatgacc cggtaggtgc ggatatcgac accctgctcg tcggccgagg
      301 ccttcgccga gacagagggt ctcacgttga agccgatgat gactgcgttc gaggcggccg
      361 ctaggttgac gtcggactcg ttgatgccgc cgacgcccga gtggataacc cggaccttga
      421 cctcagggtg agtgatgttg ttgagcgcct cttccaatgc ctcgacagat ccgcccacgt
      481 cgcccttgag cacgagcgcc aactccttga cctcgccctc cttcagacga cgatagaagt
      541 cgtcgagact cgtggccccc gccacgccct tggcgttcat ctccatgcgg agacgtgccg
      601 ttcggccttc agccatctgc ctcgcttcgc gctcatcctt gaccgcgagg gcttgatccc
      661 ccgcggcggg aacgctgttg aagccgagca cctgcgcagg gactgacggc cccgcggctg
      721 taatgacatc cccgcggaag tcgagcatcg ccttcacctt gccgtacgac tcgccgcaga
      781 cgatcgcatc gcccacgtgg agcgtgccgc gttggacgag cacggtcgcc acgggacccc
      841 gacccacgtc gagctgagac tccacgacca ccccggatgc cggagcatcc gggttggcct
      901 tgagctcctg aacgtcggcg accaggagga tcatgttcat cagttcctca agatggagtc
      961 gcttcttagc ggacacctcc accatgacgg tgtctccgcc ccactcctcg ggttgcagcc
     1021 cgtgatcgga cagctgctgc ttgacccggt cggggttggc gtcgcccagg tccatcttgt
     1081 taatcgccac aagcatcggc acgtccgccg ccctggcgtg gtcgatggct tcgatggtct
     1141 gcggcatgac gccgtcgttc gccgccacca cgatgacggc cacatctgtg accttggcgc
     1201 cgcgggccct catcgccgtg aaggcctcgt ggccgggagt gtcgatgaag gtgacccggc
     1261 gatcaccgtg atgcgcctga taggcgccga tgtgctgcgt gatccctcca gcctcgccct
     1321 tgaccacttc cgtctcccgg atcgcgtcaa gaagagaggt cttgccgtgg tcgacgtgtc
     1381 ccatgaccgt cacaaccgga gcacgctcca ccaggtcggc ggggtcgtcc tcgaaggtga
     1441 cctccccctc ctcctccgcg tgctggacct ccaccttccg ctcgaactcc tcggcgacga
     1501 tcatgatggc gtcgtcactc agcgattggg tgatggtgac caactcgccg agcttcatca
     1561 gggtcttgat gatctctcca ggcccgagct cgagggcgtc ggccaagtcc ttgaccatcg
     1621 ctccgctcgc gatcttcacg ggcgtctcgc tgccgggagc agccttcttc ttgatcggcg
     1681 ctcgttccga ctcggtgagc ccgaccggcc ctctgactcc cttaccgaca cctctaccgc
     1741 cgcgacgccc ggcctgggag tcgatgacga ccctgcggcg agatgcggtc tcgccgggct
     1801 tccacatgcg tccaccgggg gtgtaggtgc tctcgccgcc cgccttgcga cgggaagcgc
     1861 ccttcgcatc caccgcgacg gccggcgcat cagtctttgc gggcttgcgg gtcgtgggtg
     1921 gggctccggc cgcagccgga cgcggcgccg tagcacgcgg cgcggccggc ggacggcctc
     1981 ccgcctgcgg cgccttggcg gcgggcttct cttcgggctt cggcgccggg ggtgccggtg
     2041 gttcctcgcc cttgcgcacc acggtgcccg aagcattgac ccctttgcgc atgggaacgc
     2101 ccgggccgac gacagtgggg ataccctcga gacgactgcc tcggcccttc ccaccacgtc
     2161 gctgtcccgg agcggcctga gcgacgcggg ccgccgcggc ctcttgagcc gcgcgcacct
     2221 cttcggcggt agccacgatc ttcgggccga gcggaccgtc gggtttgggg gcagagcgcg
     2281 gaccgggcgc gggtctcgtc gaggacttcc gtacgccggc ttccttcgcg gcaggcttca
     2341 cgggcggcgt ggcctcggcg gccttctccg ccgcaggcga ttcggcggct accggcaagt
     2401 cggttaggcc aagcgtgccc gcagtctgga cctcgtcctc ggggcctatc gcatcatccg
     2461 gggcagccgc agactcggac gcctccgtct gttcaggcgt ctggtcctcg gcagtcacct
     2521 cggcctccga ggatgtcgac tcggcggccg gctcttctgc ggccaacggg ccaagcacgc
     2581 gttcgacgtc ggactcctcc acgctggaga cgttactctt tacttcgatg ccggcggccc
     2641 gcagacgctc cgccaactcg ggagtcccca agccacgttc cttcgctaac acgtagatcc
     2701 tcttcttcgc cactatcgct cacctgccat cggtccggtt tcttgttctg cccaacttcg
     2761 gactatcgca tgtagctccg aagcttcgac ctcgacgttc cgcctgagcg ctctctgaaa
     2821 gcctctgcgc cgaatggtct tgtccacaca caccaacttc gggcacacat aggcaccccg
     2881 gccggtccgg ctcggaccgg cctccaccac gatacccggc ggatcccgtt ccagatccag
     2941 ctggaagcgg atcaactcgt cctgacgcgc acgccgcccg cacccgagac agcgccgttc
     3001 cggctggtgc cgctcccgcg gcgccacgtg agcctctgga ctacacttcc tcgtcgtcac
     3061 cctccgctgc aagagcctgg tgggcggcta tgccgcagta tgaactgtcg ggcagagccg
     3121 cgttggggca gcgcttgccg gcgccggtaa gggcccgaca gcgaccgtcc atggtcacct
     3181 cgtcctgctc cgtcacggag aacgactgct cttcctgtga catctcggtc tcggacttga
     3241 tgtcgacctt ccacccagtg agccgattgg cgagacgtgc gttctgcccg tccctgccga
     3301 tcgcgagcgc gagttggtcg tcggggacca cgaccgtggc ctcccgagtc tgatcgtcga
     3361 ggaacacctc ccgcacgcgg gccggcgaaa gcgccttcgc gacgaaacgc gcaggctcct
     3421 cgttgaacgg gatgatgtcg atcttctccc cgcgaagctc cgacacgacc atccgcaccc
     3481 gagacccacg cggaccgaca catgccccta cggggtcgac gccgtcgctg tgagatacca
     3541 cggagatctt cgagcggtac ccgggctccc gggcgactcc cacgatctcc accagtccgt
     3601 cggcgatctc cggcacttcg agttcgaaga ggcgccggat caactcatcg ctccggcggg
     3661 agaggatgat gggcgggccc ttagggttct gctccacatc cttgatcacg gctttcaggc
     3721 gcatgccgtg ctcatagcgc tcagtgggca cttgttcgct cttcggaaga aggccttcga
     3781 ctcgtccgag gtcgaccagc gtatagcggt tgtcagactg ctgcacgatc cccgtgacga
     3841 tctcccccac ccgcgtggag tactccttga agaacaggtc ctgctcggcc tcgcggatgc
     3901 gctggtagat cacctgcttc gcggtctgtg cggctatccg gccgaagtca tcggggatga
     3961 tctcttcgcg gtcggcgatg gagtagtcta tctccacctc ttcgccctct tcgacatcga
     4021 gatccggctc caggccgggc gggaaggtca gctggaacac gtgtatctcc ccggtgtcct
     4081 ggtcgatctc gacgtcggag aagggcaccg cgttcgctgt cttgttgt
//
LOCUS       DASRMJ010000233         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_138440, whole genome shotgun sequence.
ACCESSION   DASRMJ010000233 DASRMJ010000000
VERSION     DASRMJ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138440"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFD74_06540"
     CDS             <1..269
                     /locus_tag="VFD74_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HZK49239.1"
                     /translation="AEELDGLRVSVDILDPPEDIDGIEQLDPARYGIIVRADDGRQAL
                     LLPDLEGVDTALDQLRIVCRKGGLDPARDRYHMQRFRVTRHGRH"
     gene            396..893
                     /locus_tag="VFD74_06545"
     CDS             396..893
                     /locus_tag="VFD74_06545"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HZK49240.1"
                     /translation="MPKAPRISRSRLDGFRYLSPGSDPAWRRAVGRVIDLRNVGWDVG
                     VRALERMAGFRRGERLDFHLRASARDVISGRGRAVRDEVEALADAVMKAGAYPAREEA
                     TRALAAVMSVLRERVPPELLVKLGEQLPLSEATRLRDAVLRRLEREEGGPTREGDPVA
                     GPPSG"
     gene            complement(935..3541)
                     /gene="lon"
                     /locus_tag="VFD74_06550"
     CDS             complement(935..3541)
                     /gene="lon"
                     /locus_tag="VFD74_06550"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZK49241.1"
                     /translation="MTSGERDGRDDRIDHFFDWDAAEAGQIEIVSNDAGPAAPSNAEP
                     NGGLSADDDGRTPATLPILPLKETVVFPDTMMPLAVGQPRSVRLIDDVLAKDKLVGLA
                     AVRGDDIELPGPDDTYQIGTLAAVQKMIKAPDGTLRIVVQGIRRIRIDEYVGTEPYLV
                     ARVSDLPDVLVESKELEALQRNLVSVYTKIVQLVPYLPEELELAAVNIEEPAALGYFV
                     ASTMRLKTGEKQELLELLDVAKRLRRLTEMMSSELEVLELGSKIQSQVHSEMEKTQRD
                     YFLRQQLKAIQEELGETDEAQAEVNELRQRIDEAGLPEAADKQARRELDRLAKLPAAA
                     AEYGVIRTYLDWILSLPWNVVTEDDLDIAHARRVLDEDHYDLEDVKDRILEFLAVQKL
                     KAQSSAPILCFAGPPGVGKTSLGQSIARALGRKFIRISVGGVRDEAEIRGHRRTYVGA
                     MPGTIIRALRDAESANPVFMIDEIDKMGADWRGDPSSAMLEVLDPEQHDTFRDHYLDL
                     PYDLSRIMFITTANVLDTIPGPLRDRMEIITMAGYTEEDKLHIARRYLIPRQLERHGL
                     EKKVHLEFTDGALSAIIQGYTREAGVRNLEREIGKVARKFARKVAEEGRKRLTVSPKN
                     LKDYLGTRRLFRESKRRTSDPGVSTGLAWTPAGGDILFIEARAMPGTGKLTLTGQLGD
                     VMKESAQAALTYIRSVAPTMDIAEDFFQKHDIHVHVPAGATPKDGPSAGVAMTTALAS
                     LVTGRPVDAEVAMTGEVTLTGQVLPVGGIKEKIIAARQAGITKVFLPDRNKADVDETK
                     DEVIKDMRFVYVDHVSTVLEQALEAPRRAAKAAAPPAKPRRPATKPERAAAHASRPPA
                     ESKGGRKKQVEG"
     gene            complement(3538..>3747)
                     /locus_tag="VFD74_06555"
     CDS             complement(3538..>3747)
                     /locus_tag="VFD74_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49242.1"
                     /translation="SSNAKAEYHHGFLEISLPVADRCGSRRIPILNRDQEDECEAEAE
                     TPAADAPSVDAPSAGTPDDRTGGQE"
BASE COUNT          504 a         1250 c         1285 g          708 t
ORIGIN      
        1 gcgccgagga actcgacggg cttcgcgtat ctgtcgatat cctcgaccca ccggaggaca
       61 tagacggcat cgagcagctc gatcccgccc gctacggcat catcgtgcgc gctgacgacg
      121 gtcgtcaggc cctcctgctg cccgacctcg aaggcgtgga cacggcgctc gaccaactgc
      181 gcatagtctg ccgcaagggg ggtctcgacc cggccaggga ccgctaccac atgcagcgct
      241 tccgggtgac tcgccacggc cggcactgaa accgcgcacg caaagggtgc gcctcgggtc
      301 cggtccggtc tgggcaggtc cggcccgggt cgagcccgac tctctctttc ccggcatccg
      361 acttgcgcgt atcatggtgt gtaggaggag gactcttgcc gaaggcacct cgcatatccc
      421 gatcccgtct tgacggcttc cggtacctct cgccgggaag cgaccccgcc tggcgtcggg
      481 cggttggtcg agtcatcgac ctgcgcaacg tcggctggga cgtcggcgtg cgggcgctgg
      541 agcgaatggc cggattccgt cggggagagc ggctcgattt ccacctgcgc gcctctgcgc
      601 gcgacgtcat ctcgggcagg ggaagggcgg tccgcgacga ggtggaggcg ctggccgatg
      661 cggtgatgaa agccggagcc tatccggccc gcgaggaggc cacccgggcc ctggcggcgg
      721 tgatgagtgt tctccgcgag cgcgtgccgc cggaactcct ggtcaagctc ggcgaacagc
      781 ttccgctatc cgaggccacg cgccttcgcg atgctgtgct tcggcggctg gagagggaag
      841 agggcggccc gacccgcgag ggcgacccgg tcgccgggcc cccgtcaggc tgagaccggc
      901 ggaccctccg cgggcacggt cgcgcggcgt ccgttcaccc ctcgacctgt ttcttccgcc
      961 cacccttgga ctcggccggc ggtcgcgagg cgtgggccgc ggcccgctcg ggcttggtcg
     1021 cgggtcgacg gggcttggcc gggggcgctg cggccttggc cgcccgcctc ggagcttcga
     1081 gggcctgttc gaggacggtc gagacgtggt ccacgtagac gaaccgcatg tccttgatca
     1141 cttcgtcttt ggtctcgtcg acgtcggcct tgttgcggtc ggggaggaag accttcgtga
     1201 tcccggcctg acgcgcggcg atgatcttct ccttgatccc acccaccggc agcacctgcc
     1261 cggtgagggt cacctccccg gtcatggcca cttcggcgtc caccggtcgc ccggtcacta
     1321 ggctggccag ggcggtggtc atggcgacgc cggcggacgg tccgtccttg ggggtcgcgc
     1381 ccgcaggcac gtgcacgtgg atgtcgtgct tctggaagaa gtcctcggcg atgtccatgg
     1441 tgggagccac gctgcggatg tacgtgaggg cggcctgcgc agactctttc atcacgtcgc
     1501 ccagctgtcc ggtgagggtg agtttgccgg tgcccggcat ggcgcgggcc tcgatgaaga
     1561 ggatgtcgcc gcccgcgggg gtccacgcca atccggtgga cacgcccggg tcgctggtgc
     1621 gacgtttcga ctcgcggaag agcctcctcg tgcccaggta gtccttgagg ttcttcggac
     1681 tgacggtgag tcgcttcctg ccttcctcag cgaccttgcg ggcgaacttg cgcgccactt
     1741 tgccgatctc cctctccagg ttgcgcaccc ccgcttcgcg cgtgtatccc tggatgatgg
     1801 cgctcagcgc gccgtccgtg aactccaggt gcaccttctt ctcgaggccg tgtcgctcca
     1861 gttgtcgcgg gatgaggtag cgccgcgcga tgtgcagctt gtcctcttcg gtgtatcccg
     1921 ccatggtgat gatctccatc cggtcgcgga gcggaccggg gatggtgtcg agcacgttgg
     1981 cagtggtgat gaacataatc cgagacagat cgtagggcag gtcgaggtag tggtcgcgga
     2041 aggtgtcatg ctgctcgggg tcgaggacct cgagcatggc gctcgagggg tcaccgcgcc
     2101 agtcggcccc catcttgtcg atctcgtcga tcatgaagac ggggttggcg ctctcggcgt
     2161 cgcgcagggc tcggatgatc gtgcccggca tggcgcccac atacgtgcgg cgatggcccc
     2221 gtatctcggc ttcgtcgcgc acgccaccca cgctgatgcg tatgaacttg cggccgagcg
     2281 cccgggcgat gctctgccct aggctcgtct tgcccacgcc tggagggccg gcgaagcaga
     2341 ggataggggc gctcgactga gccttcagtt tctgcaccgc gaggaactcc aggatgcgat
     2401 ccttgacgtc ctcgaggtcg tagtggtctt cgtcgaggac tctgcgggcg tgggcgatgt
     2461 cgagatcgtc ctcggtgacg acgttccacg gcagcgacaa gatccagtcg aggtaggtgc
     2521 ggatcacgcc gtactccgcc gccgcggcgg gcagcttggc caacctgtcg agttctcggc
     2581 gggcctgctt gtccgcggcc tcggggagcc ccgcctcgtc gatgcgttgt ctcagctcgt
     2641 tgacctcggc ctgggcttcg tcggtctctc ccagctcctc ttggatggcc ttcagctgtt
     2701 ggcgcaggaa gtagtcgcgc tgcgtcttct ccatctcgga gtgcacctgg ctctggatct
     2761 tcgagcccaa ctcgagcacc tcgagttcgc tgctcatcat ctcggtgagc cggcgcaacc
     2821 tcttggccac gtccagcaac tcgagcagct cctgcttctc cccagtcttc aaccgcatgg
     2881 tcgacgcgac gaagtagccg agggcggccg gctcctcgat gttcacggcc gccagctcca
     2941 gctcctccgg cagatagggc accagctgca cgatcttcgt gtagaccgag accaggttgc
     3001 gctggagcgc ctccagctcc ttggactcca ccaggacgtc ggggaggtcg ctcacccggg
     3061 ccacgaggta gggctcggtt ccgacgtact cgtcgatccg tatgcgtctg atcccttgca
     3121 ccacgatgcg gagcgtgccg tcgggggcct tgatcatctt ctgcaccgcg gcgagcgtcc
     3181 cgatctgata ggtgtcgtcg ggacccggca actcgatgtc gtcacctctg accgcggcca
     3241 agcccaccag tttgtccttc gcgagtacgt cgtcgatgag tcgcaccgac ctcggttggc
     3301 ccaccgccaa gggcatcatc gtgtcgggga acaccacggt ctctttgagg ggcaggatgg
     3361 gcagggtggc cggggtgcgg ccgtcgtcgt ccgccgagag gccgccgttc ggttcggcgt
     3421 tcgacggcgc ggcgggtccc gcgtcgttgc tgacgatctc gatctgccct gcctccgcgg
     3481 cgtcccagtc gaagaagtgg tcgatgcgat cgtcgcggcc gtcgcgttcg ccggaagtca
     3541 ctcctggcct cccgtgcggt cgtcgggcgt cccagccgag ggtgcgtcga ccgaaggcgc
     3601 gtcggccgca ggcgtctccg cctccgcctc gcactcgtcc tcctggtcgc ggttcagaat
     3661 cgggatccgt ctgctgccgc accgatccgc caccggcagg gagatctcga ggaagccgtg
     3721 gtggtattcg gccttggcgt tcgagga
//
LOCUS       DASRMJ010000234         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_345543, whole genome shotgun sequence.
ACCESSION   DASRMJ010000234 DASRMJ010000000
VERSION     DASRMJ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345543"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFD74_06560"
     CDS             complement(<1..201)
                     /locus_tag="VFD74_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZK49243.1"
                     /translation="MDQQTRHAGGCSLITNAECLIGVDRLRFHGTCLELLLRARGIDT
                     VLLVGGSTHVGIAATAFAGRDLD"
     gene            complement(162..1552)
                     /locus_tag="VFD74_06565"
                     /pseudo
     CDS             complement(162..1552)
                     /locus_tag="VFD74_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1895..>2597
                     /locus_tag="VFD74_06570"
     CDS             1895..>2597
                     /locus_tag="VFD74_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49244.1"
                     /translation="MESGDIAVLDAGNKRIQVFNKEGSFRYEVDISKASISPVDLREW
                     MGVFALLEANSSPNSVVVVDKTGTVVSRVDLPDNLADLSRALRIGRGGELLALEAGTW
                     IHPLCDPSGASLSLSAMAQRQTETALQYGEPFTLAAPGGQVKQTVTASKTGEQYEFAI
                     PGGIGGVSPLGCDRAGNYYFVVDTVSGEAGAPISVDRRVVKLDQALKPLGFAKVDLSN
                     SVVTSTRTLDVSADGD"
BASE COUNT          430 a          795 c          893 g          479 t
ORIGIN      
        1 gtccaggtcc cttcccgcga atgcggtcgc tgcgatcccc acgtgggtgg aaccaccaac
       61 caggagcacc gtgtctatgc cgcgcgcccg gagcaagagt tcgagacagg tgccgtgaaa
      121 gcggagcctg tcaaccccaa tgagacactc ggcgttcgta attagtgagc aacctcctgc
      181 gtgtcgggtt tgttgatcca cgcagcagta ggaagcgccg gaggttgtgg cggccgaacg
      241 acgaaacgct ccggagtgcg ggcgtaggct tcggccagga cgcgggcccg cttcttgtgg
      301 agttcctctg cgcgtccgta gtggacggcg gccggagtca tgaggccgat gccgccgtgc
      361 cggtgctcgt ggttgtacca ggagaagaac tcccggcaga agctgcgcgc ctcttcgatg
      421 gaagagaagc gttccgggaa gccgggccgg tatttcagcg tcttgaagtg ggcttccgag
      481 tagggattgt cggtagaggt gtagggccgg ttatgcgtcc gggtcacccc aagatcggcg
      541 agcaggaagg ccaccggctt ggaggtcatg gagctgcccc ggtcggcgtg cacggttaag
      601 gtaccgggcg tgatcttctg ctgcagggcg gcctgggcga tgagggcttt ggcgacttct
      661 ccctgttccc ggtgttgcac acaccagccc acgatgtagc gggaaaagac atcgagcacg
      721 gcgtagaggt agtagtaggt ccacttggtc ggccctttca acttcgagat atcccaactc
      781 cagacctggt tcgggcgcac ggccagaagc tccggcttct ggtagccggg gtgcgaaagc
      841 tggttgcgtc gctcccgggt ctcaccctcg gccgcgagca accggtgcat ggtgcgctcc
      901 gaggcgaggt agcggccctc gtcgaggagg gtggcgtaga ccgctgccgg ggaggagtca
      961 cagaagcgcc cgccgtgaag ctcggcgagg gccgctttgc gctcacccgt cgaaagcgcc
     1021 cgcgccggtg tgggccgcac ccgcggagtc ttctgcgttg gcggccggcg gcgacggtag
     1081 atggtggcgg gcgagacccc gagcgcccgg caggcggggc gcatccccac gaggggggca
     1141 agctcctcca cggccgcttc catcatcgtt ccgtgctcgc gtcgggctcc gcgcccctgg
     1201 gccggacaag ctcgcccaga agcgcggaga cgtttccctg gacttcgatc acccggcggg
     1261 ccttctccag ctcggcttcg gtgcgggtga gacaccggcg cagctccgcg ttctccgcga
     1321 gcagggggtc ggcgggtttg cgccctcggg cttgggagag ggccgcaaag gcgccctcct
     1381 cccgttgccg gcgccagacg gagagatgcg aggagtagag cccctcccgg cgcaggagag
     1441 caccgatctc accgggaccc gtcaggccgt cgcactcgcg caggatgccg agtttgtact
     1501 cggccgtgaa gcggcgcctc cggggccgct cgatgagttc cgggttgggg atccccgtcg
     1561 gggcggtgcc cgcggcgacc cctgcgctcc gcccgcctgc ggctcgctgc gctctggggt
     1621 cgccggctcc attctcttct gcacccttcc tctgtaacaa tgacatctac gtgctccgat
     1681 ctcgcccttc ttgctcagta attccttggt catgatcgtc tcacggtatg ttggcagaga
     1741 gggcacactc ctcttgctcc tgggagcatt cggtctgaca gaggcacgcg caaacccatc
     1801 tgtatcgttc gccatcccgc ttgggcaggg ggtagggcaa atcgcttatg tacggggctc
     1861 gaccgaaccg tgggggccaa tcagtttctg tgtcttggag agtggcgaca tagccgtgct
     1921 agacgcgggc aacaagagga tccaggtgtt taacaaagaa gggtctttcc gctatgaagt
     1981 agacatcagc aaggcgagta tcagtccggt tgaccttcgc gaatggatgg gggtcttcgc
     2041 cctcctagaa gcgaactcga gccccaactc cgtcgtggtc gtcgacaaaa cgggcaccgt
     2101 agtcagccgg gtagacttgc ccgacaatct ggccgacctg tcgcgggcgc ttcggatcgg
     2161 tcgcggcggt gaactcctcg cgttggaagc gggcacgtgg atacatccgc tgtgtgaccc
     2221 atccggcgca tccctctcac tctctgcgat ggcgcagcgg cagaccgaaa ccgccctgca
     2281 gtatggtgag ccgttcaccc tcgcagcccc cggcggacag gtaaagcaga ctgtgactgc
     2341 gagcaagacc ggggagcagt atgagttcgc cattccggga gggatcggcg gcgtgtcgcc
     2401 gcttggctgc gatcgcgcgg gcaactacta cttcgtggtc gatacggtga gtggcgaggc
     2461 gggtgcgccg atctcggtgg atcggcgggt ggtgaaactc gatcaggccc ttaagccact
     2521 tgggttcgcc aaagttgacc tttcgaatag cgtggtgaca tccacacgaa ctctcgacgt
     2581 gtcggcggat ggagatc
//
LOCUS       DASRMJ010000235         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_463954, whole genome shotgun sequence.
ACCESSION   DASRMJ010000235 DASRMJ010000000
VERSION     DASRMJ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463954"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..103
                     /locus_tag="VFD74_06575"
     CDS             5..103
                     /locus_tag="VFD74_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49245.1"
                     /translation="MRGHDGHPENERADELAVAAARQYNSHLGVLR"
     gene            120..275
                     /locus_tag="VFD74_06580"
     CDS             120..275
                     /locus_tag="VFD74_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49246.1"
                     /translation="MAADEFQRLRATRPGFWDAAAFRETLDLTDIAITEVYRDIRDRS
                     PGRDVVA"
     gene            397..543
                     /locus_tag="VFD74_06585"
     CDS             397..543
                     /locus_tag="VFD74_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49247.1"
                     /translation="MALEQGRTVPFVDGQIAATAQAHGLILVTRNVRDFELFAGLTVE
                     NWFG"
     gene            664..1170
                     /locus_tag="VFD74_06590"
     CDS             664..1170
                     /locus_tag="VFD74_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZK49248.1"
                     /translation="MNHGQETLHAPTMEFEGVDELTAQVFHAFRRAIQLQRQVVLRTL
                     SQEGGHHGEAFCLRALADNDGISQRDLASMLHLSRPRVTKMLQALEASGVVERRSDER
                     DRRLTRVFLTPEGRHRERELHARWADHINQTIGSLSEKDRRELGRLLDELSDHTSRIL
                     SDGEEPTP"
     gene            1167..2909
                     /locus_tag="VFD74_06595"
     CDS             1167..2909
                     /locus_tag="VFD74_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49249.1"
                     /translation="MKRLLLVYLRPYRLGIIVVVVLALVQALANLYLPDLNAKIINDG
                     VAKGDTAYILRMGGFMLLVTLLLGLVSVVLVYYGSRVAMGFGRDVRSGLFRKVESFSQ
                     SEINQFGTPSLITRNTNDVQQVQMLVAIGLTMMIGAPLMMIGGVIMAMRQDLPLSTML
                     VVIIPVMGVVLGLIVARAVPLFRSIQVKLDRINQVTREILTGMRVIRAFARSDYEEDR
                     FDEANADLTATGLRVTRLFALMFPALFAILNLSTVAIMYFGGGRVASGAMPIGNLTAF
                     LTYVMQILISVLMATMMLAMVPRATASADRIQQVLDVEPSIADPDAPLQVVSIDKGRR
                     GLIEFKDVEFRYPGAEHPVLCSISFSVTPGRTTAIVGSTGSGKSTLISLIPRFYDVTA
                     GSIEIDGCDIRTMARDDLWHKMGFVPQKAFLFAGTIASNLRFGAQDATDEDLWHALSI
                     AQAKEFVTEMPEGLGAPVTQGGGNVSGGQRQRLAIARALVKKPEIYVFDDSFSALDFK
                     TDSMLRAALKREVSGAAVIIVAQRVSSIMQADQIVVLETGAIAGIGTHAELLESCETY
                     REIVYSQLSAEEVA"
     gene            2906..>3696
                     /locus_tag="VFD74_06600"
     CDS             2906..>3696
                     /locus_tag="VFD74_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZK49250.1"
                     /translation="MSAREVGPGDGHPEMPAEPRGSRPLGDGSPDAPGRGVMPHGAPA
                     PGAGDDDGVKKRPPRGPGFGPPHLTGSGEKAKDFKGSFKRLAARLRPDSLKIGIVGAL
                     AVLSVALTVASPKILARATNLIFEGVISGKLPAGTTKEQVLAGLRAQGQDQLASMLSN
                     MDLTPGKGVDMGAVGRVLILVAGLYLLGAFFAWLQGYIMAGVVQRVVYRLRRDVDLKL
                     SRLPLQYFDSHARGDTLSRVTNDIDNIQQTLQQTLTQIITAILTLA"
BASE COUNT          636 a         1213 c         1196 g          651 t
ORIGIN      
        1 ctgggtgcgt gggcacgacg gccaccctga gaacgaacgg gccgacgaac tcgcggtggc
       61 ggcggcgcgt cagtataata gccatctagg agtgcttaga tagccatata ggggtgctgg
      121 tggccgcgga cgagttccag cggctgcggg ctacccgtcc gggcttctgg gacgccgccg
      181 cctttcgaga gaccttggac ctcaccgaca tagccatcac agaggtctat cgtgacatcc
      241 gggaccggag tccggggcgc gacgtggtgg catagcgccc gcggtctatc tcctggacac
      301 gaacatcctc tccgaggctc tctccgtcac gccgaacccg ggtgtcgtta ggatgctagc
      361 gcgccatcag cgcgaatcgg ccattgcggc gcccgtgtgg cactagaaca ggggcgcacg
      421 gtgccgttcg tcgacgggca gatcgccgct accgcgcagg ctcacggact catcctggtt
      481 acccggaacg tgcgcgactt cgaactgttc gccgggctca cggtggagaa ctggttcggg
      541 tagtcgcgtt gcgcactgat cgagagcgaa agccgggcct accggcctca cccccttgtc
      601 gcggaaatga ttgcgtgcaa aactgtttcg gccgaaacga aacctcaggg gatgtgcgcg
      661 atcatgaatc atggccagga gaccctccat gctccgacta tggagttcga gggcgtcgat
      721 gagctgacgg ctcaggtgtt ccacgccttc aggagggcga tccagctcca gcgccaagtg
      781 gtgctcagga cgctgtcgca agaggggggc caccacgggg aggccttctg ccttcgcgcc
      841 ctcgccgaca acgacggcat cagtcagcgc gacctggcgt ccatgctcca cctctcccga
      901 ccccgggtca ccaagatgct gcaggcgctc gaggcgtcag gggtggtgga acggcgctcc
      961 gacgagcgcg atcggcgtct cacgagggtg ttcctcaccc cggagggccg ccaccgcgaa
     1021 cgagagctgc acgcgagatg ggccgaccac atcaaccaga ctatcggcag cttgtcggaa
     1081 aaagatcggc gcgaactggg acgccttctc gacgagctct ccgaccacac gtcgcgcata
     1141 ctcagcgacg gagaggagcc caccccttga agcgccttct gctcgtctat ctcaggccgt
     1201 atcgcctggg gatcatcgtg gtcgtggtct tggcgctggt ccaagcgctc gccaacctct
     1261 acctgcctga cttgaacgcg aagatcatca acgacggcgt ggcgaagggt gacaccgcct
     1321 acattcttcg catgggcggg ttcatgcttc tggtgacgct gctgctcggt ttggtatcgg
     1381 tcgtcctggt ctactacggc tccagggtcg ccatgggttt cggtcgcgac gtgcgcagcg
     1441 gactcttccg taaggtggag agcttctcgc agagcgagat caaccagttc ggcaccccct
     1501 ccctcatcac ccggaacacc aacgacgtgc agcaggtgca gatgttggtg gccatcggcc
     1561 ttaccatgat gatcggggcg cctctcatga tgatcggggg cgtcatcatg gcgatgcgcc
     1621 aggacctgcc cttgtccacc atgttggtcg tcatcatccc ggtgatggga gtggtactcg
     1681 gtctgatcgt ggcccgcgcg gtgccgctct tccgctcgat ccaggtgaag ctcgaccgca
     1741 tcaaccaagt cacgcgcgag atcctcaccg gtatgcgggt catccgcgcc ttcgcgcgca
     1801 gcgactacga ggaggaccgc ttcgacgagg ccaacgccga cctgaccgcg accggcctgc
     1861 gcgtcactcg tctcttcgcg ctcatgttcc cggctctgtt cgcgatcctc aacttgtcta
     1921 ccgtcgcgat catgtacttc ggagggggcc gggtggccag tggcgccatg cccatcggga
     1981 atctcacagc cttcctcacc tacgtcatgc agatactcat ctcggtgctg atggcgacca
     2041 tgatgctcgc tatggtcccg cgcgctacgg cgtcggccga ccgcatccag caggtgctcg
     2101 atgtggagcc ttccatcgcc gatccggacg cgccgttaca ggtggtgagc atcgacaagg
     2161 gacggcgggg tctgatcgag ttcaaggatg tggagttccg ctatccggga gccgagcacc
     2221 cggtgctctg cagcatatcg ttctcggtga cgccgggccg gaccacggcc atcgtcggca
     2281 gcaccgggag cgggaagtcg actctcatca gtctcatccc ccgtttctac gacgtcaccg
     2341 ccgggagcat cgagatcgac ggctgcgaca tccgcacaat ggctcgcgat gatctctggc
     2401 acaagatggg gttcgttccg cagaaggcat tccttttcgc gggcaccatc gccagtaacc
     2461 tgcgcttcgg tgcccaagat gccaccgacg aggacctctg gcacgccctc tctatcgcgc
     2521 aggcgaagga gttcgtgacc gagatgcccg aggggctcgg cgcccctgtc acgcagggcg
     2581 gaggcaacgt gtcgggcggc cagcgccagc ggctggccat cgcccgcgcc ctcgtaaaga
     2641 aaccggagat ctacgtgttc gacgacagct tctcggctct cgacttcaag actgactcga
     2701 tgttgcgcgc ggcgctgaaa cgggaggtgt cgggtgctgc cgtgatcatc gtggcccagc
     2761 gggtgagcag catcatgcag gcggaccaga tagtggtgct cgagaccggc gccatcgccg
     2821 ggatcggcac tcatgcggag ctcctggaga gctgcgagac ctaccgcgag atcgtgtact
     2881 cacagctatc ggcggaggaa gtggcgtgag cgcgcgcgaa gtgggccccg gcgacggcca
     2941 tccggagatg ccggccgagc cacgcggctc ccgaccgctc ggcgacgggt cgccggacgc
     3001 cccgggccgt ggcgtcatgc cgcatggcgc ccccgcgcct ggcgccgggg acgacgacgg
     3061 tgtgaagaag cgaccgcctc gtggcccggg gttcggtccg ccccatctga ccgggtccgg
     3121 agagaaggcc aaagacttca aaggttcctt caagcgtctg gccgcgcggc tgcggcctga
     3181 ctcactgaag atcggcatcg tgggggcgct ggccgtcctc agcgtggcgc tcacggtggc
     3241 gtcgccgaag atcctggcgc gggccaccaa cctcatcttc gagggggtca tcagcgggaa
     3301 gctgccggcc gggacgacca aggagcaggt cctcgccggg ctccgtgctc agggtcaaga
     3361 ccaactggcg agcatgctct ccaacatgga cctcacgcct ggcaagggcg tcgacatggg
     3421 tgccgtgggc cgggtcctca tcctcgtcgc gggcctctat ctgctgggcg cgttcttcgc
     3481 ctggctgcag ggctacatca tggccggtgt cgtccagcgg gtggtctacc gcctgcggcg
     3541 ggacgtcgac ctgaagctca gccgactccc gctgcagtac ttcgacagtc acgcccgcgg
     3601 cgacaccttg agccgcgtca ccaacgatat cgacaacatc cagcagacgc tgcagcagac
     3661 gctcacccag atcatcacgg ccatactcac cctcgc
//
LOCUS       DASRMJ010000236         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_421207, whole genome shotgun sequence.
ACCESSION   DASRMJ010000236 DASRMJ010000000
VERSION     DASRMJ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421207"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFD74_06605"
     CDS             <1..330
                     /locus_tag="VFD74_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49251.1"
                     /translation="AALYALVVMPALVALDNAFTLRELLRDRAPQWIAQGFAPGGILV
                     LLVVIPAIVVRLRRGTAREVILALFTVMFVAAVVFTATGFLFRGPGFALFWPWQMPDG
                     YSPWNAL"
     gene            426..890
                     /locus_tag="VFD74_06610"
     CDS             426..890
                     /locus_tag="VFD74_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZK49252.1"
                     /translation="MTRRGFLRWALGAAGAALGVQGAVGFWSFFKPRIHPGAFGSKVS
                     AGQVAEFDPGTVTHVPQGRFYISRLEDAGMLALWHRCTHLGCTVPWREDEARFRCPCH
                     SSIFNEKGEVLGGPAPRPLDIFPIEIVDGEILVDTSSPIERDRFEPSQATQV"
     gene            911..2068
                     /locus_tag="VFD74_06615"
     CDS             911..2068
                     /locus_tag="VFD74_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZK49253.1"
                     /translation="MRTRDSYRPLTVAILLLMVGVFASFEVYLLREPERVAAAEARDR
                     AAATAAGQTLYADNCAMCHGEEGEGVDAPPLNDSNFLHDTPDSRIFSVIGSGVPSTEM
                     PAWNQAHGGPFTDEEIRQVVAYLRSWEDTAPDRLAEAMKGDPEEGLAVYAATCVVCHG
                     IEGEGTAQGPALNDSERLAKFDDEWYASTIADGRPAAGMPTWGTVLSPVQIRDLVALL
                     RAWERGEKVELPAPGELLHEATHELSHGDLAAVHKLLAQAADRATGQLKEAIEEAAAA
                     AESGDTDALQQALDKAIELAGSSADMGNMDTGGSGAGAMPPAPGETEARAGLVDLTAG
                     DPAAALPKLKVALALATGPLKETVEHAIADIEAGKAEEARRVLEEALPPTG"
BASE COUNT          463 a          844 c          843 g          440 t
ORIGIN      
        1 gccgccctgt acgccctggt ggtcatgccc gccctcgtcg cgctcgacaa cgccttcacg
       61 ttgcgggagc tgctgcggga ccgcgcaccg cagtggatcg cgcagggctt tgcgcccggt
      121 ggcatcctcg tgttgctcgt agtcataccc gccatcgtcg tgcgcctccg acgaggcacg
      181 gcccgcgagg tcatcttggc cctctttacc gtcatgttcg tggccgccgt tgtgttcacc
      241 gcgaccgggt tcctgttccg cggcccaggg ttcgcactgt tttggccttg gcagatgccc
      301 gacggttaca gcccctggaa cgcgctttag aaggaggacg ggtggcaccc accgaccacc
      361 gacctatcgc agcagacgga cccgccgaga tcggcggatc gggacccatc agccaaggaa
      421 gacctgtgac tcggcgaggt ttcctccgct gggcgctcgg tgctgcggga gcggcgctcg
      481 gtgttcaagg cgcagtgggc ttctggtcat tcttcaagcc ccggatccac ccgggagcgt
      541 tcggcagtaa ggtcagtgcc ggtcaggtgg cggaattcga tcccggcacg gtcacacacg
      601 tgccgcaagg acgtttttac atctcacgtc tcgaggacgc cggcatgctc gccctgtggc
      661 accgttgtac tcatctgggc tgcaccgtgc cttggcggga ggacgaagca cgcttccgct
      721 gcccgtgcca cagttcgatc ttcaacgaga agggcgaggt cctggggggg ccggctccgc
      781 gacccctgga tatctttccc attgagatcg tggacggcga gattctggtc gacacgagtt
      841 ctccgatcga gcgcgatcga ttcgaacctt cccaggcgac ccaggtctag agaggaaggg
      901 gttagacacg atgcgcaccc gtgacagcta ccgcccactc accgtcgcca tcctcttatt
      961 gatggtcggc gtctttgcgt cgttcgaggt atatctgctc cgtgagccgg agcgcgttgc
     1021 ggccgcggag gctcgcgacc gggccgccgc gactgcggcc ggccagactc tatacgcaga
     1081 taactgcgct atgtgtcacg gcgaggaggg agaaggcgtc gacgcaccgc ccctgaacga
     1141 cagcaatttc ctccacgaca ccccggactc gaggatcttc agtgtcatcg gttcgggtgt
     1201 cccgagcacc gagatgcccg cgtggaacca ggcgcatggc ggcccgttca ccgatgagga
     1261 gatccgacaa gtggtggcat acctccgttc gtgggaagac accgctcctg atcgccttgc
     1321 cgaagccatg aaaggagatc ctgaagaggg cctggccgtg tacgcggcca cctgcgtggt
     1381 ctgccacggc atcgagggcg agggtacggc gcagggacct gccctgaacg attccgagcg
     1441 cctcgccaaa ttcgacgacg agtggtatgc ctcaaccatc gccgatggac ggccggccgc
     1501 gggcatgccc acctggggca cagtgctctc gcccgtccag atccgggacc tcgtagcact
     1561 gctccgcgcc tgggagcggg gcgagaaggt ggagttgccc gccccgggcg aactcctcca
     1621 cgaagcaacc cacgagctaa gccatggcga tctggcggca gtgcacaagc tgcttgctca
     1681 ggcggccgac cgggccaccg gccaactgaa ggaggccatt gaagaagccg cggctgcggc
     1741 cgagagtggc gacaccgatg cactccaaca ggcactcgac aaagccatcg aactggcggg
     1801 gtcgagtgcg gacatgggta acatggacac gggcggctcc ggggccggcg ctatgccgcc
     1861 ggccccaggc gagaccgagg cccgggccgg cttggtcgac ctcacggccg gagacccggc
     1921 cgcagccctg cccaagttga aggtggcgct cgcgctggct acgggcccgt tgaaagagac
     1981 cgttgagcat gccatcgccg acatcgaggc gggcaaggcg gaggaagccc gccgtgtact
     2041 tgaggaggcg ctgccgccaa caggctgaac ccgcatgcga acgccgggcg gcggcggcga
     2101 cggcctcagc gaaagctaca gggaatcagc gatcctcagg ggggaccatg aagaacggca
     2161 tcggggtcaa cttccgcgac acggccatcg tcatcggagt ggcagtcgtc atcctaggtt
     2221 tcatctacct cgcgatactc ggggcttatg ccttgcggta ggagtcgtgt accctgaacg
     2281 accatccgga cgcgcacgca tcttcgagcg cctactgatt caacactcgg gcagaaatcc
     2341 gcgagggaga aatccgcggg gcgcggcgaa cgaacgcgtc gcgttccgcg atttagagcc
     2401 acgggccacc ggctccggta ggaagccacg aggtgccgca aagcgggtga ccgccgcatt
     2461 ggattcttca gaagtcacga cgcgcaactg tatgcgcacg ttcttccgtg taacctcgtc
     2521 gaacccctcg atgtcaatcg tgttctctac catagccgac aacctgcctc tctccgacat
     2581 ttctcgcggg
//
LOCUS       DASRMJ010000237         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_307752, whole genome shotgun sequence.
ACCESSION   DASRMJ010000237 DASRMJ010000000
VERSION     DASRMJ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307752"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..740
                     /locus_tag="VFD74_06620"
     CDS             174..740
                     /locus_tag="VFD74_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49254.1"
                     /translation="MTQIRILGEILDILADLHIPHMVVGSFASGFHGEFRATQDADLV
                     IDPTVEQLDALLELVSGDFYVSPEAAAEALSRRGQFNLVHFDSAWKIDLIIRKERPFS
                     REEFARRQEGNLSGRQAPVNSPEDVILAKLEWAKNSGSTRQIEDAAGVIAAWGGALDW
                     DYIDHWARVLSVEDLLAQVRSPISGNEG"
     gene            complement(782..1198)
                     /locus_tag="VFD74_06625"
     CDS             complement(782..1198)
                     /locus_tag="VFD74_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZK49255.1"
                     /translation="MRAVTVDASVWVAAADASDPRCDVSRIFLTEVGRRGFRLVAPAI
                     VLVEVGCALARRSRDAEGARRLTEAMLAPDFVSYVPMDGPLLARSFEMGTERFLCGAD
                     ALYVAAADGAGSALVSWDGEHLARGAGVSPDSWLEE"
     gene            complement(1565..2032)
                     /gene="smpB"
                     /locus_tag="VFD74_06630"
     CDS             complement(1565..2032)
                     /gene="smpB"
                     /locus_tag="VFD74_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZK49256.1"
                     /translation="MRDTGYKLIAQNKKAFHDYHIEETFEAGIVLTGTEVKSLRVGKA
                     NLRDSYASVKENELWLIGVHISPYEQGNVFNHDPLRNRKLLVHRHELKRLVGKIQRAG
                     YTLVPTKMYFKDGRAKVEVALAKGKATYDKRQDLAKKDAQRDIERGFRERHKV"
     gene            complement(2090..>2959)
                     /locus_tag="VFD74_06635"
     CDS             complement(2090..>2959)
                     /locus_tag="VFD74_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZK49257.1"
                     /translation="HGGVSEAHPSEELASHNFIEDYMVLANEQVATYLEREQVLTLYR
                     VHDAPDPFRLDHLLDLLSSLDLPTPRFDPMTATAADVRRVMIETSEWIDSSTPRQRGK
                     AALQQQVLRAQSRAVYQTQNIGHFGLSLGTYCHFTSPIRRYPDLVVHRGLLARLGLAP
                     VPTTSTLDEWAELCSVKEREASRIELTADDIALAFLLKARLDRDGWDATFDGQIVSFV
                     HAGAFVLFDRLYQGFLPARALPGDFYELDELESALVGRRTGAAFRLADLVTVRVTAVD
                     QTRGKVDLELFLD"
BASE COUNT          516 a          969 c          964 g          510 t
ORIGIN      
        1 ctgagcgagg cgttgcgcga caccatgatc gccggggtga gattccgcca ccccgagtac
       61 accgaggcgg aggcgcgctg ggccactgtc cggcacctcc tgggcgatga ccttttcgcc
      121 gcggcctatc ccgacgcgcc acgccgggca ttcgcatggg aggcgcgcgg cacatgaccc
      181 agatccgcat tctgggcgag atcctcgata tcctcgccga cctacacatc ccgcacatgg
      241 tcgtagggtc gttcgccagt ggctttcacg gagagttcag agccacacag gacgccgatc
      301 tggtgatcga tccgaccgtg gagcagctgg acgctctcct ggagctcgtc agtggcgact
      361 tctacgtctc gcccgaggct gcggctgagg cgctctcgcg ccgcggccag ttcaacctgg
      421 tgcacttcga ctccgcctgg aagatcgatc tcatcatccg caaggaacgc ccgttcagcc
      481 gagaggagtt cgcccgtaga caggagggga acctcagcgg ccgacaggcc ccggtcaact
      541 cccctgagga cgtcatcctg gccaagctgg agtgggccaa gaactccggc tcaacacggc
      601 agatcgaaga cgcagccggg gtgatcgcgg cgtggggcgg cgcactcgac tgggactaca
      661 tcgaccactg ggcgcgtgtg ctgtcagtgg aagatctgct tgctcaggtg cgatcgccaa
      721 tcagcggcaa cgaaggctga ccccaagcgc gcgctcaacg tcgcgggcta cgcctgaatc
      781 ctcactcctc cagccaggag tcgggagata cccccgcgcc tcgcgctagg tgttcgccat
      841 cccacgagac gagagcgctc cccgcgccat ccgccgccgc cacgtacagg gcgtcggcgc
      901 cacagaggaa tctttccgtg cccatttcga aggaacgcgc gaggagtgga ccgtccatgg
      961 ggacgtagct cacgaagtca ggggcaagca tcgcttcggt cagtcgcctc gcgccttcag
     1021 cgtccctgct cctgcgggcc aacgcacatc cgacctcaac aagaacgatc gcgggggcga
     1081 ccagtcggaa cccacggcga ccgacctcgg tgaggaagat ccggctcacg tcacaccgag
     1141 ggtcggaggc gtcagcggcg gcaacccaca cgctggcatc gaccgttacc gccctcatcg
     1201 cggctctagt cggccccgag cttcttccag gagagagcgc gccgtggatt ctgacggagc
     1261 gtccttcaag gtctcgtcag ccagacgaaa ccagtccgca agccactgat ccgcggtaac
     1321 acctggaagg ggcggctcgc cttccagcca tcggctgtac agctcgatgg tcacttcccg
     1381 gacggggcgg ccctcaagcg cactcttcgc cttgacccgg cggtacaggt tctcggggat
     1441 gtccaaagtc gccttcatgc cttcacgata gcaggttaac tgtattcctg gcaatactgg
     1501 ccccatggcg ccgtcccatc ggtgcctctc ccccgcgcac gccggcggct ccgagcccgc
     1561 catgctacac cttgtggcgc tcgcggaatc cccgctcgat gtcgcgctgt gcgtccttct
     1621 tggcgaggtc ctggcgcttg tcgtaggtgg ctttgccctt ggccagggcg acctccacct
     1681 tggcccggcc gtccttgaag tacatcttcg tgggcacgag agtgtagccg gcgcgctgga
     1741 tcttgccgac caggcgcttg agttcgtgac gatgcacgag gagcttgcga ttgcgcagtg
     1801 gatcgtggtt gaagacgttc ccctgttcgt aggggctgat gtggaccccg atcagccaga
     1861 gttcgttctc tttgaccgat gcatacgagt cgcgcaggtt cgccttgcct acgcggagcg
     1921 acttgacctc ggtgcccgtg agcacgatgc cggcctcgaa ggtctcctcg atgtggtagt
     1981 cgtggaaggc cttcttgttc tgggcgatga gcttgtagcc ggtgtctctc atagggcggg
     2041 aagtatagca gcggccgagg cggtgccgca cgcgggcctc aggtcgcggt caatccagaa
     2101 acagctccag atcgaccttg ccccgagtct ggtccacagc cgtcaccctc acggtgacga
     2161 ggtcggcaag gcggaaggcc gcgcccgtgc gccggcccac caatgccgac tccagttcgt
     2221 ccaactcgta gaagtcgccc ggaagcgcgc gggcgggcag gaacccctgg tagagccggt
     2281 cgaacaggac gaaagccccg gcgtggacga aactcacgat ctgtccgtcg aacgtcgcat
     2341 cccagccgtc gcggtcgagc cgggccttga gcaggaacgc caatgcgatg tcgtcggcgg
     2401 tgagttctat gcgggacgcc tcccgctctt tgaccgagca gagctcggcc cactcatcga
     2461 gagtcgacgt ggtaggaacc ggagccagcc ccaggcgggc caagagaccg cggtgcacca
     2521 ccaggtcggg ataccgccgg atgggcgagg tgaagtgaca gtaggtgccc aacgacagac
     2581 cgaagtggcc aatgttctgg gtctggtaga cggcgcgact ctgggcgcgc agcacctgct
     2641 gctgcagcgc cgccttgcca cgttgccggg gcgtcgagga gtcgatccac tccgacgtct
     2701 cgatcatgac ccggcgcacg tctgcggcgg tggcggtcat ggggtcgaac cgcggggtgg
     2761 gcagatcgag cgacgacagc agatcgagca gatggtcgag ccggaaggga tcgggagcat
     2821 cgtgcacccg gtagagagtc agcacctgct cgcgttcgag ataggtggcc acctgctcgt
     2881 tggccaggac catgtagtct tcgatgaagt tgtggctggc gagctcctcg gaggggtggg
     2941 cctcgctcac tcctccgtg
//
LOCUS       DASRMJ010000238         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_54990, whole genome shotgun sequence.
ACCESSION   DASRMJ010000238 DASRMJ010000000
VERSION     DASRMJ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54990"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFD74_06640"
     CDS             complement(<1..463)
                     /locus_tag="VFD74_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZK49258.1"
                     /translation="MRTWRIAELLEVSTGFLRERGSSSPRLDAELLLADALGVGRIDL
                     YTHFDRPLGADEVDSYRALVARRGKREPVAYILGRASFRYLTLKVTPATLIPRPETEE
                     LVDAVLEWLRLHPVLEAGGCGAAADEAVAGTIVSPRPTPLSVPAVVDVGTGS"
     gene            complement(495..1571)
                     /gene="prfA"
                     /locus_tag="VFD74_06645"
     CDS             complement(495..1571)
                     /gene="prfA"
                     /locus_tag="VFD74_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZK49259.1"
                     /translation="MIEKLVREIIERQEAITAELSDPAIFDDQARYTEVSRAHSDLAD
                     AYRLAAEYRAAQAAAAEAEVMLSETDLDPDMREFIQEERRQALEDLERIDEDIRTASL
                     DKDPRDEKNVIVEVRAAAGGDEAALFAAEIYRMITKYAERLRFKHEVLTTSESPIGGL
                     KEIIFEVKGRGAYGVLKWESGVHRVQRVPVTESTGRIHTSTATVAVLPEVQEVEISID
                     PNELRVDVYRSSGPGGQSVNTTDSAVRITHLPSGLVVSCQDEKSQLQNKEQAMRILRT
                     RLQAMADEEKDAKEAATRRSQVGTGDRAQKVRTYNFPQGRVTDHRVGLTSHRMESILE
                     GELEEFTEALVAWDRAERLTEATF"
     gene            complement(1628..2713)
                     /locus_tag="VFD74_06650"
     CDS             complement(1628..2713)
                     /locus_tag="VFD74_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZK49260.1"
                     /translation="MSEHKNATGGQAAGHTHAVDCAATQRAVGGDSATQHAVGCPVAE
                     HSVGGQAVIEGVMMRGKRSWGLAVRQPSGVIARHSFALDPVGDRYPVLKLPVLRGVVT
                     LVDSLVLGMKALGLSANLSLDGDPETAEAGDVESAAEPKAPAGLGWKEMAATFVVAIG
                     LAVVLFVVVPLAVVKYFEQTFSNPFVFNLVEGLIRIAIFLAYVGAISFIPDLRRVFEY
                     HGAEHKVIHAYEAGEELDPAHAGRFTTLHPRCGTAFLLVVMVMAIIVFSIVGKPSLPL
                     LVLSRLVGIPIVAGLSYEVIRFAGRHKDGVISKVVLWPGLKLQRLTTREPSADQIEVA
                     IAALMEVLRVDAGGEPDPRGMVLPGAK"
     gene            complement(2706..3332)
                     /locus_tag="VFD74_06655"
     CDS             complement(2706..3332)
                     /locus_tag="VFD74_06655"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HZK49261.1"
                     /translation="MRQLPQRDGWVEVICGSMFSGKSEELMRRLRRASIAGQTIIVVK
                     PALDVRYDATRISSHDGRGWDAHCVQRADEILLLPDIESADVIAVDEAQFLDDELPHV
                     AAFLADRGKRVIVAALDQDFRGEPFGPIPRVMATAEFVDKLQAICARCGAPASRTQRL
                     IDGRPAGEHDPLIKVGGTESYEARCRSCHEVRRVDNLSMWGADGGSDV"
     gene            complement(3351..4103)
                     /gene="thyX"
                     /locus_tag="VFD74_06660"
     CDS             complement(3351..4103)
                     /gene="thyX"
                     /locus_tag="VFD74_06660"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZK49262.1"
                     /translation="MRVSLLQHTPAPDRAVAAAGRLCYAPLSAADLHEEMGAQDVVRM
                     VRILVGSGHHSALEHASFTFAVDGISRACSHQLVRHRLASYNQQSQRYVRFGTGDDFV
                     VPPNIDAVPAAKAVFLEAMEQARHAYDKLVELGLEQGYANETVQEDARFVLPNAAETK
                     IVVTMNARELRHFFRVRCCRRAQWEINRLAWAMRHLVTAVAPTLFEKTGPGCVIDSCP
                     EGKMACGTEYSEVEIQEMDFSSVVARPVTQST"
     gene            complement(4128..4337)
                     /gene="rpmE"
                     /locus_tag="VFD74_06665"
     CDS             complement(4128..4337)
                     /gene="rpmE"
                     /locus_tag="VFD74_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZK49263.1"
                     /translation="MKTDIHPEYMESNVHCSCGETFTTRSTMPDIKVEICSVCHPFYT
                     GKQRLVDTGGRVERFNKKLGRTTQS"
BASE COUNT          820 a         1619 c         1524 g          802 t
ORIGIN      
        1 cgctcccggt tcccacgtcc acgaccgccg gcactgacag gggagtcggg cgtggggaca
       61 caatggtgcc tgcgacggct tcgtccgcgg cggccccaca gccgcccgcc tccaagaccg
      121 ggtgtagccg cagccactcc aggaccgcgt ccaccaactc ttccgtctcg gggcgaggga
      181 tcaacgtggc cggggtgact ttcagcgtga ggtagcggaa ggaagcgcgg cccaggatgt
      241 aggcgacggg ctcccgcttc ccccgacgcg cgaccagcgc ccgataggaa tcgacctcgt
      301 ctgcgcccag aggccggtcg aagtgggtgt agagatcgat gcggcccact ccgagcgcgt
      361 cggccagcag cagttctgcg tccaatctcg gcgaggagga gccgcgctcg cgcaagaagc
      421 cggtcgagac ctcgaggagt tccgctatcc gccaggtgcg catgcgccgt ccggccgccg
      481 ggggagaccc gccgctagaa cgtggcctcc gtcagccgct cggcacgatc ccaggcgacc
      541 agagcctcgg tgaactcctc cagctctccc tccaggatgg actccatccg atgggaggta
      601 aggcccaccc ggtggtccgt cacccggcct tgcgggaagt tgtaggtgcg caccttctgt
      661 gcccggtctc cggtgcccac ttggctgcgt cgggtcgcgg cctccttggc gtccttctcc
      721 tcgtcggcca tggcctgcag gcgcgtacgc aggatgcgca tggcctgttc cttgttctgc
      781 agctgtgact tctcgtcctg gcacgacacc accagccccg acggcagatg tgtgatcctg
      841 acggcggaat cggtcgtgtt caccgattga ccgccgggtc cgctcgagcg gtagacgtcg
      901 acgcgaagct cgttggggtc gatgctgatc tcgacctctt ggacctcggg caacaccgcc
      961 accgtggccg tggaggtgtg gatgcgtccg gtggactcgg tcacgggcac gcgctgcacc
     1021 cggtgcactc ctgattccca cttgaggacg ccatacgcgc cccggccctt gacctcgaag
     1081 atgatctcct tgagacctcc gatgggtgac tcgctcgtgg tgagcacctc gtgcttgaac
     1141 cggagtcgtt ccgcgtactt ggtgatcatg cggtagatct cggcggcgaa cagcgcagcc
     1201 tcgtcgcccc ccgccgccgc gcgcacctcc acgatgacgt tcttctcgtc gcgggggtcc
     1261 ttgtcgagac tggcggtgcg gatgtcttcg tcgatgcgct ccagatcctc cagagcctgc
     1321 ctgcgctcct cctggatgaa ctcacgcata tcgggatcga ggtccgtctc gctcagcatc
     1381 acctcggctt cggcagcggc agcctgcgcc gcgcgatact ccgcggccaa gcggtaggcg
     1441 tcggctaggt cggagtgcgc tctcgacacc tcggtgtacc gcgcctggtc atcgaagatc
     1501 gcggggtcgg acaactccgc cgtgatggct tcctgtcgct cgatgatctc tctgaccaac
     1561 ttctctatca ttcttctctt cttcctttgg agcccgcgtc agaacaggcg cagcctcaga
     1621 ctgagcccta cttcgcgccc ggcagcacca tgccccgcgg gtcgggctct cccccggcgt
     1681 cgacccggag gacctccatc agtgccgcga tggccacctc gatctggtcc gccgaaggct
     1741 cccgggttgt gagacgctgc agcttgaggc caggccacaa cacgaccttc gagatcacgc
     1801 catccttatg cctacccgcg aaccggatca cctcgtacga gagccccgcc actatcggga
     1861 tccccaccag acgcgacaac acgagcaacg gcaggctcgg tttgccgaca atcgagaaca
     1921 cgatgatcgc catgaccatg acgacaagga ggaaggccgt tccgcagcgc ggatgcagcg
     1981 tggtgaaacg gccagcgtga gcaggatcga gttcctctcc ggcttcgtac gcgtggatga
     2041 ccttgtgctc cgccccgtgg tactcgaaga cgcgccgcag atcggggatg aacgagatgg
     2101 cgcccacgta cgcgaggaag atggcgatcc ggatgagccc ctcaaccagg ttgaagacga
     2161 acgggttgga gaaagtttgc tcgaagtact tcaccactgc cagcggcacc acgacgaaca
     2221 gtacgaccgc gagaccgatg gccaccacga acgtcgccgc catctccttc cagccgagac
     2281 ccgccggagc cttcggctcg gccgcgctct cgacgtcacc ggcctccgcc gtctccgggt
     2341 caccatccag actcaggttg gccgagaggc ccagggcttt catgcccagc accaacgagt
     2401 ccacgagggt caccaccccc cggaggacgg gcagcttcag cacggggtag cggtccccga
     2461 caggatcgag tgcgaaggag tggcgcgcga tgactcccga aggctgccgc accgccaacc
     2521 cccacgagcg tttgccccgc atcatcacac cctcgatgac cgcctggcca ccgacggagt
     2581 gctcggccac cgggcagcca acggcgtgct gagtcgccga gtcgccaccg acggcgcgtt
     2641 gggtcgccgc gcagtcgacg gcgtgcgtgt ggccagccgc ctgaccgccc gtcgcgttct
     2701 tgtgctcaga catcagaccc accgtcagcc ccccacatgc tcagattgtc gacgcgtcgc
     2761 acctcgtggc aagaacgaca ccgtgcttcg taggactcgg tgccgccgac cttgatcaga
     2821 gggtcgtgct cgccggccgg acggccgtcg atgagccgct gcgtgcgcga cgccggcgcg
     2881 ccacatcggg cgcatatggc ctgtagcttg tcgacgaact cggcggtggc catcacccgg
     2941 gggatggggc cgaacggctc tcccctgaaa tcctggtcga gcgccgccac gatgacccgc
     3001 ttgccgcggt cggccaggaa cgcggccacg tgcggaagtt cgtcgtcgag gaactgggcc
     3061 tcgtccacgg cgatcacgtc ggcgctctcg atatccggaa gcagcaggat ctcgtcggcg
     3121 cgctgcacgc agtgggcgtc ccaaccgcga ccgtcgtggg agctgatgcg ggtggcgtcg
     3181 taccgcacgt ccagcgccgg cttcaccacg atgatggtct ggccggcgat ggaggcgcgg
     3241 cgcagtcgtc gcatgagctc ttcgctcttg ccactgaaca tgctgccaca aatgacctct
     3301 acccagccgt cgcgctgggg caactgccgc atcgatctct cccggggtac ctaggtggat
     3361 tgggtgaccg ggcgggcgac caccgaggag aagtccatct cctggatctc gacctcagaa
     3421 tactccgtgc cgcaggccat cttgccttcg ggacaggaat cgatcacgca cccaggaccg
     3481 gtcttctcga agagagtggg cgcaacggcg gtgacgagat gccgcatggc ccacgccaac
     3541 cgattgatct cccactgggc ccgacgacag cagcgcacgc ggaagaagtg cctcagttcg
     3601 cgggcgttca tggtgaccac gatcttggtc tcggcggcat tgggcaggac gaagcgggcg
     3661 tcttcctgca cggtctcgtt ggcgtagccc tgctccagcc ccagttccac cagcttgtcg
     3721 tacgcgtggc gggcctgctc catggcttcg aggaagaccg ccttcgccgc cggcaccgcg
     3781 tcgatgttgg gaggcacgac gaagtcgtcg ccggtgccaa accgcacgta gcgctgcgac
     3841 tgctggttgt acgacgcgag acggtgacgc accaactggt gcgagcaggc ccgggagatg
     3901 ccgtcgaccg caaacgtgaa cgaggcgtgc tcaagcgcgc tgtgatggcc cgagcccacc
     3961 aggatgcgga ccatgcgcac gacatcctgc gcgcccatct cctcgtgaag gtcggccgcg
     4021 ctgaggggcg catagcacag gcgcccggca gcggccacag ccctgtcagg cgcaggggtg
     4081 tgttggagga gtgatactcg cagtgaggcc ggcacgcgct tcccggacta gctctgggtg
     4141 gtgcgcccga gcttcttgtt gaagcgctcc acccggccgc cggtgtcgac aagacgctgc
     4201 ttacccgtgt agaacggatg gcagacggag cagatctcca ccttgatgtc gggcatggtg
     4261 gatctggtgg tgaacgtctc gccgcacgag cagtggacgt tgctctccat gtattccggg
     4321 tgaatgtcgg tcttcatgag tcctccgatg tttcacggcc tgggccgggg acgttctggt
     4381 tgaaagtgag cgagtaatga tagcacgggg cggcaaggcc gtcgccttcg cgagttgctc
     4441 gacgctgagc gggaggagga agccgagctg aagcggtgag ctgggctgag agcagcccgg
     4501 aaggcgactt acagaacttc tgagacacaa cccaagctca gaccaaaggt gcatagcatc
     4561 cacccaaccc gtgaagcatg ctcactacta catgcctggc aagggatcat gaactcagcc
     4621 gatctctgcg cgtgccccga aggcgaggac aagacggtga tcgaaaacgc accacgacca
     4681 acagcgccaa gtcgcctcgt tgtcaccgtt ccccgcacgg ctctcgcggc accgcgtcgc
     4741 gccccaaccc cggcacggtc aaatg
//
LOCUS       DASRMJ010000239         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_163760, whole genome shotgun sequence.
ACCESSION   DASRMJ010000239 DASRMJ010000000
VERSION     DASRMJ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163760"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFD74_06670"
     CDS             <1..429
                     /locus_tag="VFD74_06670"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZK49264.1"
                     /translation="RAPAAGSSGAGARSTVHGTTGSGPDLVLLGLGENGHTASLFPHA
                     TALGEQERWVLEVFVDAAAGAGTSAAGADMWRVTTTAPFINRAAAIVFLVAGVAKTAT
                     VKEVIEGPYDADRLPAQLISPAGGDVRWYLDTEAGSLLVR"
     gene            598..2613
                     /locus_tag="VFD74_06675"
     CDS             598..2613
                     /locus_tag="VFD74_06675"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZK49265.1"
                     /translation="MATFISSPFTVGLSEELPPAESFAEGIRRAPARGFTLSPADTVV
                     AVKNALRYVPVNLHEALAPEFLHELRTYGRIYAYRYRPRGDIAAKPIDEYTGILEARA
                     LQLNIDNNLAFDVALYPYELVTYGETGQVFQNWLQYRLVKQYLEVMTEEQTLVIASGH
                     PVGLFPTHRNAPRVINTNGLMVGEFDNPRDFHYVTQMGVANYGQMTAGGWMYIGPQGI
                     VHGTYLTLLNAGRLYLGIPDDGDLAGRLFVTSGLGGMSGAQPKAADIAGAVSITAEVD
                     SSRIKTRLEQGWVQRASADLSEVFAWADQARAARTALSIAYHGNVVDLWEYVVRESIP
                     VELASDQTSCHAPYDGGYVPQGTSFEESRELLARDPDEFALRVNASLARQFDLIGVMA
                     SRGTHFWDYGNAFLKAVFDAGVLRVAANGSDTRDGFVFPSYVEHIMGPLCFDLGYGPF
                     RWVCTSGDPSDLRKTDRAAMECIDPGRCGQDRDNYIWIRDAEKNRLVVGSQARILYSD
                     AEGRVRIALRFNDMVRHGEIGPIMIGRDHHDVSGTDSPYRETANIYDGSRMMSEMAHH
                     SWAGDAVRGMTLVVLSNGGGTGVGKAVNGGFGLVLDGSERVDGIIRSALEWDVVGGLA
                     RRSWARNAPAMETVAAWNQAHRENGRVTVPYLVEDGLLETLVKGGHA"
     gene            2615..>3049
                     /locus_tag="VFD74_06680"
     CDS             2615..>3049
                     /locus_tag="VFD74_06680"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZK49266.1"
                     /translation="MELWYTHLLRETIDQDSLLVQGNRACALGALDAGIRFFAGYPIT
                     PSSEVMEVMARELPLVGGVFLQMEDEIASICACIGASLTGGVAMTATSGPGFSLMQEA
                     LGYAIMAEAPLVVVNVMRMGPSTGRPTAPSQGDVMQARWGTHG"
BASE COUNT          488 a         1023 c         1041 g          497 t
ORIGIN      
        1 cgggcgcccg ccgccgggtc ctccggggcg ggcgcccgat cgaccgtgca cggcacgaca
       61 ggctcgggtc cggatctcgt gctgctgggc cttggcgaga acgggcacac ggcctccttg
      121 ttcccgcacg cgactgccct cggggagcag gagcgctggg tactggaggt gttcgtggac
      181 gcggcggccg gcgcgggcac cagcgccgcc ggcgccgaca tgtggcgggt cacgaccacc
      241 gctcccttca tcaatcgagc ggccgcgata gtgttcttgg tagcgggcgt ggcgaagacc
      301 gccacggtca aggaggtgat cgagggaccg tacgacgccg acaggctgcc cgcgcagctc
      361 atcagtccgg cgggcggcga cgtgcgctgg tatctagaca cggaggcggg gagtctcttg
      421 gtgcggtagg gggcgcctcg taggcgaggt gtgggcgccg ccgaccgccg ggcgcgggcg
      481 cgccgggtcc tgcgggccgg acttgggtat catgtcgtgc aggctgcgtc gttcggtccg
      541 ccatacgggg tcacgagccc gcgaacagcg cacctcacgc tacgagaaga gggaacgatg
      601 gccactttca tctcgagtcc cttcacggtg ggtctctccg aggagcttcc gccggcggag
      661 agcttcgccg aggggatccg gcgggcgccc gcccgcggct tcacgctgtc tccggccgac
      721 acggtggtcg ccgtcaagaa tgccctgcga tacgttccgg tgaatctgca cgaggcgctc
      781 gccccagagt tcctccacga gctgcgcacg tatggccgca tctacgccta ccgctaccgg
      841 ccccggggcg acatcgcggc gaagccgatc gacgagtaca cgggcatcct ggaggcccgg
      901 gcgctccaac tcaacatcga caacaacctc gccttcgacg tggccttgta tccctacgag
      961 ttggtcacct acggcgagac cggccaggtg ttccagaact ggctgcagta ccggctggtc
     1021 aagcagtact tggaggtgat gaccgaggag cagaccctcg tgatcgcctc cggtcaccct
     1081 gtgggtctct tcccgaccca tcggaacgct ccgcgggtga tcaacaccaa cgggctcatg
     1141 gtcggcgaat tcgacaaccc gcgcgacttc cactacgtca cgcagatggg cgtggccaac
     1201 tacgggcaga tgaccgccgg cggatggatg tacatcggtc cgcaaggcat cgtccacggc
     1261 acctatctca cgctgctcaa tgccgggcgc ctgtatctgg gcatccccga cgacggcgac
     1321 ttggcggggc ggctcttcgt cacctctgga ctcggcggca tgagcggggc tcagcccaag
     1381 gcggccgaca tcgccggcgc cgtgagcatc accgccgaag tggattcatc caggatcaag
     1441 acccggctcg aacagggttg ggtgcagcgg gcctcggcgg acctctccga ggtctttgcc
     1501 tgggccgacc aggcccgtgc ggcgcgcacc gccctctcga tcgcctacca cggtaacgtg
     1561 gtcgacctgt gggagtacgt tgtgcgcgag agcatcccgg tggaactcgc cagcgaccag
     1621 acctcctgtc acgcacccta cgacggcggc tacgtccccc agggcacgag ttttgaggag
     1681 tcgcgcgagc tcctagcccg cgatcccgac gagttcgccc tgcgcgtgaa cgcgtcactc
     1741 gcgcgccagt tcgatctcat aggcgtcatg gcgtcacgag gcacccactt ctgggactac
     1801 ggcaatgcct tcctcaaggc ggtgttcgac gcgggcgtgt tgagggtggc ggcgaacggc
     1861 agcgacacgc gggacggttt cgtcttcccc tcctacgttg agcacatcat ggggccgctc
     1921 tgcttcgatc tgggctacgg accgttccgc tgggtgtgca ccagcggcga cccatccgac
     1981 ctaaggaaga ccgaccgggc tgcgatggag tgcatcgacc ccggccgatg cgggcaggat
     2041 cgcgacaact acatctggat ccgcgacgcc gagaagaacc ggctggtcgt ggggagccag
     2101 gcccgcatcc tctactccga cgcggagggg agggtgcgca tcgcccttcg cttcaacgac
     2161 atggtgcggc acggcgagat cgggccgatc atgatcggcc gcgatcatca cgacgtgtcc
     2221 gggaccgact ccccctatcg tgagaccgcg aacatctacg acggctcacg tatgatgtcg
     2281 gagatggcac accattcgtg ggcgggtgac gcggtgcgtg gcatgacctt ggtggtactc
     2341 tccaacggag gcggcacggg agtgggcaag gcggtcaacg gcgggttcgg attggttttg
     2401 gacggctcgg agcgcgtcga cggcatcatc cgcagcgctc tggagtggga tgtcgtgggc
     2461 gggctggcac gacggagttg ggcgcgcaac gctcccgcca tggagaccgt ggccgcctgg
     2521 aaccaggccc accgcgagaa cgggcgcgtc accgtgccgt acttggtaga ggacggactg
     2581 ttggagacac tcgtgaaggg cggccacgcc taagatggaa ctctggtaca cccacctcct
     2641 gcgggagacc atcgaccaag acagcctgct cgtgcaaggc aaccgggcct gcgccctcgg
     2701 ggccttggac gccggcatcc gcttcttcgc cggctacccc atcaccccct cctccgaggt
     2761 catggaggtc atggctcggg agctgcccct ggtggggggc gtgttcctgc agatggagga
     2821 tgagatcgcc tccatctgcg cctgcatcgg ggcctccctc accggagggg tggccatgac
     2881 cgccacctcc ggtcccggct tctccctcat gcaggaggcc ctgggctacg ccatcatggc
     2941 cgaggctccc ttggtggtgg tgaacgtcat gcgcatgggc ccctccaccg gtcggcccac
     3001 cgccccctcc caaggcgacg tcatgcaggc ccgctggggc acccacggt
//
LOCUS       DASRMJ010000240         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_163904, whole genome shotgun sequence.
ACCESSION   DASRMJ010000240 DASRMJ010000000
VERSION     DASRMJ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163904"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..725
                     /locus_tag="VFD74_06685"
     CDS             228..725
                     /locus_tag="VFD74_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HZK49267.1"
                     /translation="MLATLVVALVVVALFGAMELGLLSLLSGDSAGAGSTNTTVAAGA
                     TESTAPVTSGSGAPTTTARAATTTLLASTTTTTAAPVAVPFSLVLKAKEDVWVEFRDA
                     ASGEALFVGMVAKDESKTLDAAGPVNAVVGKPGALSLRVEGRSVEPPNSFRWLVSSTG
                     AEDRP"
     gene            887..2491
                     /locus_tag="VFD74_06690"
     CDS             887..2491
                     /locus_tag="VFD74_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZK49268.1"
                     /translation="MDAHELPADQLETIELGDYLRVIRERKWIIIAAVAVVAAAALMF
                     SLLSTPKYRASSQLLYKVSNLDVTLFGSRVFEVRDLNRELQTGAGMVRLNTVADAVAQ
                     ELSSGYTTEQLLGMVDVRPDAQTNFIDITATSVHPAEAADIADAFAQKFVDSRRVADR
                     AVVAEARKLVNEEIASLTPLEAASERGLMLRDKLQELEILESMQTGGYEIVQLATVPI
                     SPFSPQPVRNGILGLAVGLVLGLGLAFLLEYLDRRIKDDDVMAHEYGVPVLARVPMVG
                     KKWVHSSGARSAAPIGFMSAESAILESFRTLRSNLHYFEVDRPLHTLMITSALPQEGK
                     TVTSVNLGLSLALSGSKVIILETDLRRPQLQNYLQIGNDVGISNVLAGSHTFAEAMQL
                     VRVDDFIPAESRRSGPGNGGSSGSLMQKNLYCITSGPLPPNPAELLASAKMAELLTVA
                     AEAADYVIVDTPPLLLVSDGLAISNLVDGVILTSRMRNSTVDQAREVRQVLARVSART
                     IGVVATGVPRTKGYYSRYGGYAGYGY"
BASE COUNT          409 a          851 c          809 g          426 t
ORIGIN      
        1 ccgcggcccc tcccgccaca ccttcgacac ccgccgcggc cccgcccgcg cccgcggcgt
       61 ccgagccgac acccgacttc ttccgtccga cgccacgccg ggagaagaag cctcccagct
      121 ccgccacgtc ccacgtcggc gctcccgaag tgtcgaggag atcacgacca gacccgcgcc
      181 gcgggtctgg ggaaggtcgt gttcggcccc ccctagttcg agtggccgtg ctggccaccc
      241 tggtggtcgc gctcgttgtt gtcgccctct tcggggccat ggagctcggg ctgctgtcgc
      301 ttctcagcgg cgactccgcg ggtgccggaa gcacgaacac gaccgtggcg gccggtgcga
      361 cggagagcac agcgcccgtg acgtccggat cgggtgcgcc tactacgact gcccgcgcgg
      421 cgaccacgac gctcctggcc tcgaccacta cgacgacggc cgctccggtc gccgtcccgt
      481 tctctctcgt cctgaaggcg aaggaggacg tgtgggtgga gttccgggac gcggcgtccg
      541 gtgaggcgct gttcgtgggc atggtcgcca aagacgagtc gaagacactg gatgccgcgg
      601 gccccgtgaa cgcggtcgtc ggcaagcctg gggcgctgtc tctccgggtc gagggcagga
      661 gcgtggaacc gcccaactcg ttccgctggc tcgtgagttc caccggggca gaagaccgac
      721 cttgacgcgc tcgatgcgca gcgtatcggg ttgcagcggg cgccgccccg gcgcatcaga
      781 tcggaggtcc agcgaggtcg tacggggcgc ggcgcgggcg cccgccgatc gcctcttact
      841 tcgattgctt gtatgcatga cggtccatac actaaactag ccactcatgg atgcccacga
      901 actacccgcg gaccagctcg agaccatcga actcggtgac tatctgcgag tcatccgaga
      961 gcgcaagtgg ataatcattg cggcggtcgc ggtagtagcg gcagcggcac tcatgttctc
     1021 tcttctaagc actcccaagt accgggcttc gtcccagctc ctctacaagg tctcgaatct
     1081 ggacgtcacg ctgttcggct cccgcgtctt tgaggtgcgc gacctgaacc gcgagctgca
     1141 gaccggcgcg ggaatggtgc gcctcaacac cgttgcggat gcggtcgccc aggagttgag
     1201 ttccggctac accaccgagc agcttctggg aatggtcgat gttcgccccg atgcgcagac
     1261 caacttcatc gacatcacgg ccaccagcgt ccacccggcc gaagcggccg acatcgcaga
     1321 cgccttcgcg cagaagttcg tggactcccg tcgcgtcgcc gaccgcgccg tggtcgccga
     1381 ggcccggaag ctggtgaacg aggagatcgc ctcgctcacg ccgctcgagg cggcttccga
     1441 gcgcggactc atgttgcgcg acaaactcca ggaactcgag atcctcgagt ccatgcagac
     1501 cggcggctac gagatcgtac agctggcgac cgtgcccatc tcgcctttca gtccgcagcc
     1561 ggtgcgcaac gggatactcg gcctcgccgt cggcttggtc ctgggactgg gtctggcctt
     1621 cctgctcgag tatctcgacc ggcgcatcaa ggacgacgac gtgatggcgc acgagtacgg
     1681 cgtgccggtg ctcgcgcgtg tcccgatggt ggggaagaaa tgggtgcatt cgagcggtgc
     1741 gcgctccgcc gcgccgatcg gattcatgtc ggccgagtcg gccatcctcg agtcgttccg
     1801 cacgctgagg tcgaacctgc actacttcga ggtggaccga cccctgcaca cgttgatgat
     1861 cacgagcgcg ttgccccaag aggggaagac tgtgacttcg gtcaatcttg gtctcagctt
     1921 ggcgctctcg gggtccaagg tgatcatcct ggaaacggac cttcgtcggc cgcaactcca
     1981 gaactacctc cagatcggca acgacgtggg gatctccaac gtcttggcag gctctcacac
     2041 cttcgcggag gccatgcaac tggtgcgcgt cgatgacttc attccggctg agagccgcag
     2101 gtccggaccc ggcaacgggg ggtcgtccgg ttcactgatg cagaagaacc tctactgcat
     2161 cacctccggc cccctcccgc cgaaccccgc cgaactactg gcgagcgcga agatggctga
     2221 actcctcacg gtggcggccg aggcggccga ctacgtcatc gtcgatacgc cgccgctcct
     2281 gctggtgtcc gacgggctgg ccatatcgaa cctggtggac ggggtcatcc tcacctcccg
     2341 catgcgtaac agcaccgtgg accaggcgag agaggtgcgc caggtgctgg cacgagtctc
     2401 agcgcggacc atcggtgtgg tcgcgaccgg ggttccgcgc accaagggct actactcgcg
     2461 gtacggcggc tacgccgggt acggctacta ggcgg
//
LOCUS       DASRMJ010000241         7851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_200254, whole genome shotgun sequence.
ACCESSION   DASRMJ010000241 DASRMJ010000000
VERSION     DASRMJ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200254"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFD74_06695"
     CDS             complement(<1..795)
                     /locus_tag="VFD74_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HZK49269.1"
                     /translation="MPEDTTHPGPAEVHLRLFATAARGLEPLLAGELRALGAAGVKEV
                     RAGCSFAGTIETAYRVCLWSRLASRVLLPLAEVPAADADDLYEGVKALSWEHHLAPTG
                     TLAVDFTASGSSITHTHYGALRVKDGIVDRFRESTGVRPSIDIRHPDLRVYAHATAET
                     AVVGLDLSGAALHRRGYREEGGQVLAPLKENLAAAILVRAGWPEIAAAGGGFLDPLCG
                     SGTLPIEAALMVGDVAPGLLRSTWGFTGWRQHDASVWTRLLDEARAR"
     gene            944..2470
                     /locus_tag="VFD74_06700"
     CDS             944..2470
                     /locus_tag="VFD74_06700"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HZK49270.1"
                     /translation="MLAASGIAVLTGLWIESKSNYTLFHTLAEMFTVAVSFSIFMITY
                     NTRRFLRNNYLLVLGTALLFIGFVDLLHALTYKGVEVFSADDSNRPTQLWLAGRYLQA
                     GVLLAAPAALTRATIKTRHILPILALLTAGLLASIFVWPIMPTAFGASGLTTFKVVSE
                     YAICVVLLGALLLLLLHRRHFEPRVTAMLAGYLALTILSELAFTLYSDPYGNANLAGH
                     LIRILAVYLAYKAIVVTGLVKPYTLLFRELKISEERHRAGEERFEQIANVLQEALLTV
                     PDEVPGIEFGHLYQSATYATRVGGDFYDLFELDGGRIAVIVGDVSGKGLPAATLTSRV
                     KDTIRAYSMIGDSPAEVLARTNSILVRSTDTSSWVSVFLGYLDVHSGRLVYCSAGHPP
                     SVIRRVQAAGGDGKATFFLETSSPILAAFEDASFVNAEEQLAPSDILLLYTDGVVEAR
                     RGEELFGDDRLLESVARLGSEGASHLPGRLFAKIKDFASGRLSDDTAIVAISRRAATL
                     "
     gene            2519..2938
                     /locus_tag="VFD74_06705"
     CDS             2519..2938
                     /locus_tag="VFD74_06705"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZK49271.1"
                     /translation="MDDTAESTVDRIRTIVENDRLIRHFGMTVDEASEGYARVSVEVR
                     EDFLNVHDTAHGALVFALADVAFAITVNSIVDAMGVQWSFNILRSARAGDVLTAECSI
                     VHRGSRLLVVGYTVKKGDGVLVAQGQATALPVRNTAR"
     gene            complement(2994..3980)
                     /locus_tag="VFD74_06710"
     CDS             complement(2994..3980)
                     /locus_tag="VFD74_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZK49272.1"
                     /translation="MSSPSSKKAITAAVLGNLAIAVTKFSAAAVTGSSAMLSEGIHSV
                     VDTGNGLLLLHGIRRSRRPADAEHPFGHSQELYFWTLVVAVMIFAVGGGVSMYEGVIH
                     IRHPQPLEDPFWNYVVLAFAMVFEGISFTVAWREFGRARAGRGVWRAVRDSKDPSLFT
                     VVFEDSAAMMGLVLAFLGVLLGHLLENPYLDGAASVAIGAVLAAVAAVLAYESKGLLL
                     GEAADPEVVGEVTALAADDPAVVEVEHVLTMHLGPTDILLNLKVRFVPGLSSLQVGRV
                     VDRLEAALRAAHPEIKRIYIEGGVGEEGDRAGQQGGASEEGGAVEQGRPADR"
     gene            4405..7614
                     /locus_tag="VFD74_06715"
     CDS             4405..7614
                     /locus_tag="VFD74_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HZK49273.1"
                     /translation="MATLGIDIGCISVKMAVVGEEADRESFERLACDHPLFQREGKGT
                     APTPGATPPILATTYRRILGSPVETARGLLSEVLEALPGDAVTGMRVTGSGARLVANG
                     LGAGVENEFKAIARAVGSLHPEVTTVFEMGGETSKFIQLETDADTGRVGISDYQTNGD
                     CAAGTGSFMDQQANRLLYDIEDVGDIVCGAGKAATIAGRCSVFAKSDMIHAQQKGYQP
                     PEVLKGLCNAVIRNYKGSIAKGKSVEGKVAFIGGVAANEGAVGALLEAFELPRERLLI
                     PADYAWMGAIGAALIEADEASGREPMVIDLQGLGTQQTESLPVSDPLSMERVILLRDR
                     VKPYVLPDVGVIDAYLGIDIGSVSTNLVVLDSDGEVVKEIYMKTDGRPVEVVNKGLAE
                     IWEEMGPQLNIRGVGTTGSGRELIGELAGADTVNDEITAHKTGATFIGRKLLDRIPDT
                     IFEIGGQDSKFISLQDGVVIDFAMNEACAAGTGSFLEEQAEKLGINIIGEFAEMGLAA
                     TAPVRLGERCTVFMERDVNSYLQRGAEKRNVVAGLAYSIVYNYLNRLVGTRHIGDTIY
                     FQGGTAYNDAVAAAFSQILERDIIVPPYNGVVGAIGMALLAREKMEATAAVSRFRGWD
                     LEQVDYTIREFTCKGCSNECDIRQFTIGDEKTYWGDKCSDRYRKRAKVDKKPVIQDLL
                     ALRERAAFSYVLDDAVLSTAMHRTQGDANGEDVVADGNGGAPAVKGIDLTGPVRSGSG
                     ARLGTMGIPRSMYTYDRLPFWAVFFKECGFDVLVSGESNKSIREAGVEHSVAEPCFPI
                     RVAHGHVSDLFERGAEYVFVPNVLNEETDHPELESHACPWGQTLPWVVRAAPHLEQYA
                     DRLVTPTLHFRRGREYLKESLRADMKKLGVPPKAVDSAVDKAYQAQADYKRTLQEAGR
                     DALATLEEHGEMGIVLIGRSYNIYDPGINMDIPAKLRKFYGVNVIPLDFLPLWDTPTR
                     HIVPNMFWNYGRRNIQAAMFVHEHRNLHMIYITNFKCGPDSYIKHFVRKATPDPFLTL
                     QFDEHTNDAGAMTRCEAYLDSKGFLRWWARDKQSASQPA"
     gene            7625..>7851
                     /locus_tag="VFD74_06720"
     CDS             7625..>7851
                     /locus_tag="VFD74_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49274.1"
                     /translation="MSIETARAGGNGRATSEAATRALAAMPVPGELRSADSSPMASAD
                     IDLTGRTLFLPRMPYGGSRLMAAAMASIGIEA"
BASE COUNT         1382 a         2694 c         2514 g         1261 t
ORIGIN      
        1 tcgcgcccgc gcctcgtcga gcaaccgagt ccacaccgag gcgtcatgct gtctccagcc
       61 ggtgaagccc cacgtgctgc gcagcagccc aggagccaca tcgcccacca tcagtgccgc
      121 ctcgatgggc agagtgcccg atccgcacag gggatccagg aacccgccac cggcggcggc
      181 gatctccggc catcctgcgc gcacgagtat cgccgccgcc aggttctctt tgagcggcgc
      241 cagcacttgg cccccctcct cgcgataccc gcgacgatgc agggccgccc ccgacagatc
      301 caggcccacg acggcggtct ctgccgtcgc gtgcgcatag acgcggaggt cggggtggcg
      361 gatgtcgatc gaggggcgga ctccggtgga ctcgcggaac cggtcgacga tgccgtcctt
      421 gacacgcagg gcgccgtagt gggtgtgcgt gatggacgag ccggacgcgg tgaagtccac
      481 ggccaacgtg ccggtgggag cgaggtgatg ctcccaggag agcgccttca ctccctcata
      541 caggtcgtcg gcatcggcgg ccggcacctc ggccagcgga aggagcaccc ggctcgccag
      601 ccgtgaccac aggcacaccc tgtaggcagt ttctatcgtg cccgcgaaac tgcagcccgc
      661 tctcacctcc ttgacgcccg ccgcgccgag agcgcgcaat tcgccggcga gcagcggttc
      721 gagcccgcgc gccgcagtgg caaacaagcg caggtggacc tcggccggtc cgggatgagt
      781 tgtgtcttcg ggcatgcgtc tccttgcctg gcggagcacc tggtcaggtc tcaagcctcc
      841 gcagcgccta tgatactctc cagactgagg cgaccctgcg ggaggtagcg tgacaccttt
      901 cacaccagag cctaggtcac ggtggacgac agccaggccg ttcgtgttgg cggcctccgg
      961 gatcgcggtc ctcacgggac tttggatcga gtcgaagagc aactacacgc tcttccacac
     1021 cctggccgag atgttcacgg tggccgtctc gttctccatc ttcatgatca cctacaatac
     1081 gcgccggttc ctccggaaca actacctgct ggtcctgggc accgccctgc tcttcatcgg
     1141 gttcgtggac ctcctccacg ctctcacata caagggtgtg gaagtgttct ccgcggacga
     1201 ttccaaccgc ccgacccaac tgtggctggc gggtcgctac ctccaggcgg gtgtgctgct
     1261 tgcagccccg gcggcgttga cccgcgcgac gatcaagacc cggcacatac tgccgatcct
     1321 cgcgcttctc accgccgggc tactcgcctc gatcttcgtg tggccgatca tgccgacggc
     1381 ctttggcgcg agcggcctca ccacattcaa ggtcgtgagc gaatacgcga tctgtgtggt
     1441 gctgctcggt gcgctccttc ttctcctgct ccaccgccgt cacttcgagc ctcgggtgac
     1501 ggccatgctg gccgggtatc tcgcgctcac catcctctct gagttggcgt tcaccctgta
     1561 ctccgatccc tacggcaacg ccaacctggc gggacatctc atccggatct tggccgtcta
     1621 cctggcgtac aaggcgatcg tcgtgacggg gttggtgaaa ccctatacgc tgctcttccg
     1681 cgagctcaag atcagcgagg agcgtcaccg agcgggtgag gagcgtttcg agcagatagc
     1741 caacgtgctc caggaagctc tccttaccgt gcccgacgag gtgccgggca tcgagttcgg
     1801 ccacctctac cagtccgcca cctacgccac gagggtcggg ggagacttct acgatctgtt
     1861 cgaattggat ggcggccgca tcgccgtgat cgtgggtgac gtctcgggca agggactccc
     1921 ggcggcgacg cttacgtccc gggtcaagga caccatccgg gcctacagca tgatcggcga
     1981 ctcgccggcc gaagtactcg cgcgcaccaa ctcgatcctg gtgcgctcta ccgacacctc
     2041 ctcgtgggtg agcgtgttcc tcggctacct ggacgtgcac tcgggacggc tggtctactg
     2101 ctcggccggg catcctcctt ctgtgatccg gcgggtgcag gcggccggcg gcgacggcaa
     2161 ggcgaccttc ttcctggaga cctcgtcgcc gatactcgcg gcgttcgagg atgcgtcctt
     2221 cgtgaacgcg gaggaacagc ttgcgccgag tgacatcctc ctcctgtaca cggacggtgt
     2281 tgtcgaagcc cgccgaggcg aggagttgtt cggggacgac cgactcctcg aatccgtggc
     2341 ccgcttaggc tcggagggcg cctcacatct cccggggcgg ctcttcgcca agatcaagga
     2401 cttcgcctcc ggacgcctgt cggacgacac ggccatcgtg gcgatctcgc ggcgggctgc
     2461 gacgctgtga agcggcttcg tcgttcgggg accacccgca tcggctagaa tctccgacat
     2521 ggatgacacc gcggagagca ctgtggacag gatacggacc atcgtggaga acgatcggtt
     2581 gatccgacac ttcggaatga ccgtagacga ggcctcggaa ggctacgccc gcgtgtcggt
     2641 cgaggtgcgg gaggatttcc tcaacgtgca cgacaccgcc cacggcgccc tggtgttcgc
     2701 gctcgccgat gtcgccttcg ccatcaccgt gaactcgatc gtcgacgcca tgggggtgca
     2761 gtggagcttc aacatcctgc gtagcgcccg cgccggcgac gtgctcaccg ccgagtgctc
     2821 gatcgtccac cgtggttcgc gattgctcgt ggtgggctac accgtgaaga agggagacgg
     2881 ggttctcgtc gcccaaggac aggcgaccgc gctgcctgtc agaaacaccg cccgctgacc
     2941 gaagacccgt gccccggcct tcgccggggg ccgggggccg cgcgcggccg cggctatctg
     3001 tcggccgggc ggccctgttc gactgcaccg ccttcttcgc ttgcgccacc ctgttgaccc
     3061 gcgcgatcgc cctcttcgcc cacgccgccc tcgatgtaga tgcgtttgat ctcagggtgc
     3121 gcggcgcgga gagcggcctc cagcctgtcc accacgcggc ccacttggag ggacgacagc
     3181 ccgggaacga acctcacctt caggttgaga aggatgtcgg tgggacccaa gtgcatggtg
     3241 agcacgtgct ccacctccac cacggcgggg tcatcggcgg cgagcgctgt gacctccccc
     3301 accacctccg ggtcggcggc ttctcccaag agcagaccct tgctctcgta ggccaagacc
     3361 gcggccaccg ccgcgagcac cgccccgatc gccaccgacg ccgccccgtc gaggtaggga
     3421 ttctccagca gatgtccgag aaggactccg aggaaggcca gcacgagccc catcatcgcc
     3481 gccgaatcct cgaacacgac ggtgaacagc gacgggtcct tgctgtcgcg caccgcgcgc
     3541 cagaccccac gcccggcgcg ggcgcggccg aactcccgcc aggccacggt gaacgagatg
     3601 ccctcgaaca ccatcgcgaa ggcaagcacc acgtagttcc agaacgggtc ctccaacggt
     3661 tgcgggtgcc ggatgtggat cacgccttca tacatcgaca cgcctccgcc cacggcgaag
     3721 atcatcacgg cgaccacaag cgtccagaag tacagctcct ggctgtggcc gaaggggtgc
     3781 tccgcatcgg cggggcggcg gcttcgtctg atcccgtgca ggagcaggag cccattcccc
     3841 gtgtcgacga cagagtggat gccttccgag agcatggccg agctgccggt aacagcggcg
     3901 gcggagaact tcgtcacggc gatggccagg ttccccagca ccgccgccgt tatcgctttc
     3961 ttcgatgatg gtgaggacac gctacctcca aggtcttggg cgagatcgcc cggttcggcc
     4021 cctgggcctt ccccgggagg cgccgcttca ggggcgccca ggaagacgcc tgaagcgaac
     4081 gccaggcgct cggcgcggga ctccctcgcc gagcgacact gtacccgaag caggagcggc
     4141 agaccaggaa ctgcctgctc gggggcgtcc accagaatcc gggggcgccc ttcgcgggcg
     4201 catgggcgtg aggcttccgt ggtaagctgt agcgcgatca atcgcggcag aaccactgcg
     4261 gtcgtgggcg atgcgccggt cggggctgaa cgccggagcg gcgattcgcg aaccctgatt
     4321 ctccggtctc cagagtgcgg acggccgcga ggcacgggct gcgtgcaccc cacaccgttc
     4381 caagcagaag gagaccggca atctatggcc acgctcggaa tcgacatcgg ttgtatcagc
     4441 gtcaagatgg cggttgtggg ggaggaagcc gatcgcgagt cgttcgagcg tctcgcctgt
     4501 gaccaccctc tcttccagcg cgagggtaag ggcaccgccc cgacgcccgg agcgacgcca
     4561 cccatactcg ccaccaccta ccgccgtatc ctcggcagtc ctgtggagac cgcccgcggc
     4621 ctcctctccg aggttctcga agctctcccg ggcgacgccg tcacgggcat gcgtgtcacc
     4681 ggctcaggcg cgcgcctcgt ggcgaacggc ctcggcgccg gtgtggagaa cgagttcaag
     4741 gccatcgcac gcgccgtcgg ctccctgcat ccagaggtca ccacggtctt cgagatgggc
     4801 ggcgagacct ccaagttcat ccagctcgag accgacgccg acaccggccg ggtgggcata
     4861 tccgactacc agaccaacgg cgactgcgcc gcgggcaccg gctcgttcat ggaccagcag
     4921 gccaaccgcc tgctctacga catcgaagac gtgggcgaca tagtctgcgg cgccggcaag
     4981 gcggccacca tcgccggacg gtgttcggtg ttcgcgaagt cggacatgat ccacgctcag
     5041 cagaagggct atcaaccgcc ggaggtgctc aagggcctct gcaacgcggt tatccgcaac
     5101 tacaagggca gcatcgccaa agggaagagc gtcgagggca aggtcgcctt catcggtggg
     5161 gtcgccgcca acgagggcgc ggtgggagcg ctgctcgaag ccttcgaact gccccgcgag
     5221 cgactgctca tccccgccga ctacgcatgg atgggcgcca tcggcgccgc tctcatcgag
     5281 gcggacgaag cctcggggcg tgagcccatg gtcatcgacc tgcagggtct gggcacccag
     5341 caaacggaga gcctgcccgt ctccgatcca ctcagcatgg aacgggtcat cctcctacgc
     5401 gaccgtgtga agccctacgt gctccctgac gtgggcgtca tcgacgccta cctgggcatc
     5461 gacatcggct cggtctccac caacctggtg gtgctcgact cggacggcga agtggtcaaa
     5521 gagatctaca tgaagaccga cggacgtcca gtcgaggtgg tcaataaggg gctggccgag
     5581 atctgggaag agatggggcc acagctgaac atccgaggcg tcggcaccac gggctcgggc
     5641 cgcgaactca tcggggagtt ggccggggcg gacaccgtca acgacgagat caccgcccat
     5701 aagaccggag ccaccttcat cgggcgcaag ctcctcgacc ggatccccga caccatcttc
     5761 gagatcggcg gccaggactc caagttcatc agcctgcagg acggcgtggt gatcgacttc
     5821 gccatgaacg aggcctgcgc cgccggcacc gggtcgttcc tggaggagca ggcggagaag
     5881 ctgggtatca acatcatcgg cgagttcgcc gagatgggcc ttgcggcaac ggccccggtt
     5941 cgcctcggcg aacggtgcac ggtcttcatg gagcgcgacg tcaactccta cctccagcgg
     6001 ggggcggaga agcgcaacgt ggtggcgggg ctggcctact cgatcgtcta caactacctg
     6061 aaccgcctgg tgggaacacg ccacatcggc gacaccatct actttcaggg tggcaccgcc
     6121 tacaacgacg cggtagcggc ggctttctcg cagatcctcg agagggacat catcgtgccc
     6181 ccctacaacg gagtggtggg cgccatcggc atggccctgc tcgcccgcga gaagatggaa
     6241 gccaccgccg ccgtctcccg gttccgcggt tgggacctcg agcaggtgga ctacaccatc
     6301 cgcgagttca cctgcaaggg ttgctcgaac gagtgcgaca tccgccagtt cacgatcggc
     6361 gacgagaaga cgtattgggg cgacaagtgc tccgaccgct accgcaagcg cgccaaggtg
     6421 gacaagaagc cggtgatcca agacctgctc gcgctgcgcg aacgtgcggc cttctcctat
     6481 gtgctcgacg acgcggtgct cagcactgcc atgcaccgca ctcagggcga cgcgaacggc
     6541 gaggacgtcg tggccgacgg gaacggcggc gcccccgcgg tgaagggcat agatctcacg
     6601 ggaccggtgc gcagcgggtc gggagccaga ctgggcacca tgggcatccc gcgcagcatg
     6661 tacacctacg accgcctccc tttctgggcc gtcttcttca aggagtgcgg gttcgacgtg
     6721 ctcgtgtcgg gcgagtcgaa caagagcatc cgcgaggccg gggtggaaca ctccgtggcc
     6781 gagccctgct tccccatccg agtcgcccac gggcacgtgt ccgatctgtt cgagcgcggc
     6841 gccgaatacg tgttcgtgcc caacgtgctg aacgaggaga ccgaccaccc cgaactcgaa
     6901 tcgcacgcct gcccctgggg acagacgctg ccctgggtgg tgcgggccgc accgcacctg
     6961 gagcagtacg ccgaccggct cgtgactccc acgctccact tccgtcgcgg aagagagtac
     7021 ctgaaggagt ccctacgcgc cgacatgaag aagctcggcg ttccccccaa ggcggtggac
     7081 tccgccgtcg acaaggccta ccaggctcag gcggactata agaggacgct gcaggaggcg
     7141 ggccgggacg cgctcgcgac cttggaggag cacggggaga tgggcatcgt gctcatcggc
     7201 cgcagctaca acatctacga tccgggcatc aacatggaca tcccggccaa gctgcggaag
     7261 ttctacggcg tcaacgtgat acccctcgac ttccttccgc tctgggacac ccccacgcgc
     7321 cacatcgtgc ccaacatgtt ctggaactac ggtcggcgga acatccaagc ggcgatgttc
     7381 gtgcacgaac atcgcaacct gcacatgatc tacatcacca acttcaaatg cggcccggac
     7441 tcctacatca agcacttcgt gcgcaaggcg actccggatc cgttcttgac gctgcagttc
     7501 gacgaacaca ccaacgacgc cggcgccatg acgcggtgcg aagcctacct cgacagcaaa
     7561 gggttcctgc gctggtgggc gcgcgacaag caatcggctt cgcaaccggc ctagggagat
     7621 acacgtgagc atcgagacag ccagagccgg cggcaacgga cgagccactt ctgaagcggc
     7681 gacgcgggcg ctcgccgcca tgcccgtgcc gggtgaactc cgcagcgccg actccagccc
     7741 catggcgagc gccgacatcg acctcaccgg ccgcactctg ttcctgccgc gcatgcccta
     7801 cggggggtcg cgcctcatgg ccgccgccat ggcttccatc ggcatcgaag c
//
LOCUS       DASRMJ010000242         5901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_239859, whole genome shotgun sequence.
ACCESSION   DASRMJ010000242 DASRMJ010000000
VERSION     DASRMJ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239859"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /locus_tag="VFD74_06725"
     CDS             <1..1632
                     /locus_tag="VFD74_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK49275.1"
                     /translation="FLREEIETMKMFGNRDVKRGGTPNRVLVIISILTMLLLAVAIAA
                     GCSSSSDTTTTAADTGTVTRGGVLKVGMLPGNGQYDPVMMAGSVGDIILNVQVLENLV
                     DLAQDFSMTPVLATEWSSEDGKTWKVTLRDGVKFTNGEAFTADDVVYSFDRLRGKDSP
                     MADVYSNIESVVADDPTHVTFSLTTPDSEFIGSLTDYRSKMLSKSVADPMTELVGTGP
                     YMLESFAAEDRAVLKANPDYWGTDEKGEKLPYLDGVEFIYSPDTAGQIEGLQGGSLNW
                     VGGLTAAQKETVEANSSLKTISTATNYANELQIRTDVEPGSKLAFRQALLYGTDLQAM
                     IDLVAPGVGDAGNGTLVGPAYASYYLDESVPYDPAKAKELLAEAGYADGVDIKLVAQT
                     SDPIPALATAWQAQMKEIGVNVEIQQVPTDVYYAEDGQDNWYQAPFSIVDWGTRASPI
                     TYFKLALTSTAPWNYSRWSNAEFDSLVEQIPQTLDDAARADLYKQAQKILQTEVPMIN
                     FIVNLGVAGESNNVDGIAVAPDWAQTLFRTANLSK"
     gene            1848..2720
                     /locus_tag="VFD74_06730"
     CDS             1848..2720
                     /locus_tag="VFD74_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49276.1"
                     /translation="MEDIRDATPRTTMRTLYWRRLKRRPVALVGVAVFSFFLLLALFG
                     PWIAPFDYTAQSAALRLHPPSIEHPFGTDQFGRDILSRIIVGTRNIFLLGGIGTLLAV
                     LIGTPIGLISGYVGGLVDEVIMRLLDVLLSFPSLLLALVLLSVLGPSNLNLVLVVTVL
                     YTPMLARVVRSMVLDLKTKEFVEAARVRGERRGYILFREILPNSLPPLLVETSMRFSY
                     SIFLVASLGFLGLGVQPPSPDWGLQINEARNFFSLAPWTLLFPAGTIALLVIATSLMS
                     DGLRQVMLPGVARD"
     gene            2722..4908
                     /locus_tag="VFD74_06735"
     CDS             2722..4908
                     /locus_tag="VFD74_06735"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HZK49277.1"
                     /translation="MDGESRAAIHATAPAAGRPTAPDSATAPATRRPVFEVEGLCVEY
                     ETEAGVLRAVRDVSLSVHEHESFGLVGESGSGKSTLAMGAIRYLARNGRITAGSVRLN
                     GVELLGLSPKELRGLWGSSIGVVYQSPLSALNPSIQIGKQLAEVARLHLGMDRAAARA
                     RVTEMLSKVAMPDPEAVMKRYPHQLSGGMLQRCVIAMALMTNPALLIMDEPTTALDVT
                     TQAVVLDLVADLKREFDSAILYITHDLGVVTKICERIGVMYAGEFMEQASLRALFQRP
                     LHPYTLDLLGCVPRFETTPVRRSLVTIPGSIPRLDELPTGCVFAPRCSLVQDACRAER
                     PPLVEIEDGHRTACRRWRDVPTPTEYLCEATEALSPMESSDEAAVALVEVDDTYTHFR
                     GPGSGLIPGRRRYRAIRAVDGVSFTVDEGRTLGIVGESGSGKTTVARAIIGLTPATGG
                     DIRLRGEKLERSTGRRRRDVLKEIQMVFQNPDASLNPTRSVGDAITRPLVLLGGLGGK
                     DAREQAEGLLRAVSLPAGYFDRLPNELSGGEKQRVAIARAFAADPDLILCDEPISSLD
                     VSVQGALMNLLMTLQQEKRTSYVFISHDLSAVQHLSDTIAVMYLGRVMEMGDSVRVLT
                     PPFHPYTEALLSAVPVADPDVTQTPIRLGGTVPSAMDVPAGCRFHPRCPRRLGDICCT
                     EEPPWRHGEGEHRIYCHIPLEELAHLQEKTLVLAAGEEPALREKDV"
     gene            4908..5852
                     /locus_tag="VFD74_06740"
     CDS             4908..5852
                     /locus_tag="VFD74_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49278.1"
                     /translation="MLHFIARRLGFVVLMVILSSTIIFAATQVLPGDVATMMLGRFAS
                     EQAKENLREELGLNEPLPSQYVHWLGSFATGDWGRSVSTRTEIRPLILQRLGNSAKLA
                     AVAFAIFVPLGILLGLVAALRRNTWVDQVISVGSLAFIGLPEFVSAVILISIFSIQLG
                     WLPSQSAIDPKAGFIESFRFLILPGVTVALTSLAYIVRMTRSSTVDVLQTDYVRTANL
                     KGLPPHRVLFTHVLRNSLLPTITVIAISVGWLIGGLIVTESVFGYPGLGRLVLFAVQR
                     RDIPLIQATVVVVVVIYGVSNLLADILYAVLNPRIRYT"
BASE COUNT         1000 a         2038 c         1862 g         1001 t
ORIGIN      
        1 ttcttgcgag aggagataga gactatgaag atgttcggca atcgcgatgt gaagcgaggt
       61 ggaacaccca accgggtcct cgtcatcatc agcatcctga cgatgctgtt gctggcggta
      121 gccatcgcgg cgggctgcag cagctcgagc gacaccacca ccactgcagc cgacaccggc
      181 accgtcacca ggggcggcgt gctcaaggtc gggatgctgc ccggcaacgg acagtacgac
      241 ccggtcatga tggccggcag cgtcggcgac atcatcctga acgtccaggt gctggagaac
      301 cttgtcgacc tggctcagga cttcagtatg actccggtgc tggccaccga gtggagttcg
      361 gaagacggca agacctggaa ggtcactctg cgggacggcg tgaagttcac caacggtgaa
      421 gccttcaccg ccgacgacgt ggtctactcc ttcgaccgtc tgcgtgggaa ggactccccg
      481 atggccgacg tgtactcgaa catcgagagc gtcgtcgccg acgaccccac tcacgtgacg
      541 ttcagcctca cgacgcccga ctctgagttc atcggctccc ttaccgacta tcgttccaag
      601 atgctcagca agagcgttgc ggatccgatg accgaactcg tcggcaccgg cccctacatg
      661 ctcgaatctt tcgccgccga ggaccgcgcg gtactcaagg ccaaccccga ctactggggc
      721 accgatgaga agggcgagaa gctcccctat cttgacgggg tcgagttcat ctactcgccc
      781 gacacggccg gccagatcga gggcctgcag ggtggctcac tcaactgggt cggcggcctg
      841 accgcggcgc agaaagagac ggtcgaggcc aactcgagcc tgaagaccat cagcaccgcc
      901 accaactacg ccaacgagct tcagatccgc accgacgtcg agcccggaag caagctcgcg
      961 ttccgccagg cgctgttgta cggcaccgac ctccaggcca tgatcgatct ggtcgccccc
     1021 ggtgtaggcg acgccggtaa cggcacgctg gtcggcccgg cctatgcgag ctactacctc
     1081 gacgagtcgg tcccgtacga ccccgcgaaa gcgaaggaac tgctcgcaga agcgggatat
     1141 gccgacggcg tcgacatcaa gctggtcgcc cagacgtccg atcctatccc cgccctcgcg
     1201 actgcctggc aggcgcagat gaaggagatc ggggtcaacg tggagataca gcaggtgcca
     1261 acggacgtct actacgctga ggacggccag gacaattggt atcaagcccc cttcagcatc
     1321 gtcgactggg gcacccgcgc gtcgcccatc acgtacttca agctggctct cacgagcacg
     1381 gctccgtgga actactcgcg ctggagcaac gccgagttcg acagcttggt ggagcagatt
     1441 ccgcaaacgc tcgacgatgc ggcccgcgcc gacctctaca agcaggctca gaagatactg
     1501 cagaccgaag tgccgatgat caacttcatc gtcaacctcg gggtcgcggg cgagtcgaac
     1561 aacgtggacg gcatcgcggt ggcacctgac tgggctcaaa cgctcttcag gactgctaac
     1621 ctcagtaagt aacctgcgcc ggacgtactg agcgaacgct cggatcgtcc ggatcgttcg
     1681 ggtattcgag agggggccga catcggcccc ctctcgccga gacgcccgaa accatgggcg
     1741 gactgcatat atgattatcg accgacagtc gctgactgga aggccacggt gtgaacgaac
     1801 ccgcaggaga cggcggtccg acccaagaaa agggcgcgac gccgcagatg gaagacatac
     1861 gagacgccac tccgcgcacg acgatgcgca ccctctattg gcggcgtctc aagcgccgcc
     1921 ccgtggctct ggtgggtgta gccgtcttca gcttcttcct gctgctggcc ctgtttggac
     1981 cctggatcgc ccccttcgac tacacggccc agtccgcggc gctgcgtctt catcccccgt
     2041 ccatcgagca ccccttcggc accgaccagt tcggtcgcga catcttgagc cggatcatcg
     2101 tgggcacgcg caacatcttc ctgctcggcg ggatcgggac gcttctggcc gtgctcatcg
     2161 gcacccctat cggcctgatc tcgggctacg tgggcggact ggtggacgaa gtgatcatgc
     2221 gcctcctcga cgtgctgctc tccttcccct ccctgctgct cgccctggtg ctcctcagcg
     2281 tgctcggacc ctccaacctc aacctggtgc tggtagtcac cgtcctctac acgcccatgc
     2341 tcgcccgggt ggtgcgcagc atggtgctcg acctcaagac caaggagttc gtcgaggcgg
     2401 ccagggtccg cggcgagcga cgcggctaca tcctcttccg cgagatactc ccgaactccc
     2461 tgcctccact cctggtggag acctcgatgc gcttctccta ctcgatattc ctggtcgcct
     2521 ccctgggctt cttgggcctg ggggtgcagc ctccctcgcc cgactggggg ctgcagatca
     2581 acgaagcgcg caacttcttc tctctcgccc cgtggacgct gctcttcccg gccggaacca
     2641 tcgcgctcct ggtgatcgcc accagcctca tgtccgacgg cctgcggcag gtcatgctgc
     2701 cgggcgtcgc gagagactga cgtggacggg gagtctcgcg ccgccatcca cgccaccgcg
     2761 cccgcggcgg ggcggccgac ggcgcctgat tccgccaccg cacccgcgac ccggcggccg
     2821 gtcttcgagg tggagggtct gtgcgtggag tacgagaccg aagcgggcgt gctgcgcgcc
     2881 gtgcgggacg tctccctgag cgtgcacgag cacgagagct tcggcctggt gggagagtcg
     2941 ggctcgggca aatccacgct ggccatgggc gccatccgct acctggcgcg caacggacgc
     3001 atcaccgcgg gcagcgtccg cctgaacggg gtggagttgc tcggcctttc gcccaaggag
     3061 ctccgcgggc tgtggggatc cagcatcggc gtcgtctacc agagcccgct cagcgccctc
     3121 aacccctcta tccagatcgg caagcagctg gccgaggtgg cgcggctgca cctgggcatg
     3181 gaccgcgccg cggccagggc gcgcgtcacg gagatgctct cgaaggtggc catgcccgac
     3241 cccgaagccg tgatgaagcg ctacccccac cagctctccg gggggatgct gcagcgctgc
     3301 gtcatcgcta tggcgctcat gaccaacccg gcgctcctca tcatggacga gccgaccacc
     3361 gccctcgacg tgaccacgca ggctgtggtg ctcgacctgg tggccgacct caagcgggag
     3421 ttcgactccg ccatcctcta catcacccac gacctgggtg tggtcaccaa gatctgcgag
     3481 cggatcgggg tcatgtatgc gggcgagttc atggagcagg cctccctgcg ggccctgttc
     3541 caacgaccgc tgcaccccta tacgctcgac cttctcggct gcgtgccccg cttcgagacg
     3601 actccggtga gacgatccct ggtgaccatc ccgggctcca tcccccgcct cgacgagctg
     3661 cccacggggt gtgtcttcgc accgcgctgc agtctcgtgc aggatgcctg ccgggcggag
     3721 agaccgcccc tcgtggagat cgaagacgga catcgcaccg cctgtcgccg ctggcgcgac
     3781 gtgccgacgc cgaccgagta cctgtgtgag gccaccgaag cgctctcccc catggagtcg
     3841 agcgacgagg cggcggtcgc gctggtcgag gtggacgaca cctacacgca cttcagggga
     3901 ccgggcagcg gactcatccc cgggcgccgc agataccgcg ccatccgggc cgtcgacggg
     3961 gtcagcttca ccgtcgacga aggccggaca ctcggcatcg tgggcgagag cggcagtggg
     4021 aagaccaccg tcgcccgggc catcatcggg ctcacgccgg ccacgggcgg ggacatccgc
     4081 ctgcgcgggg agaaattgga gcgcagcacc ggcaggcggc ggcgcgacgt gctcaaagag
     4141 atccagatgg tgttccagaa ccccgacgcc tccctcaacc ccacccgcag tgtgggagac
     4201 gccatcaccc ggcccctggt tctgctcgga ggactcggcg ggaaagacgc ccgggagcag
     4261 gccgaagggc tcttgcgggc cgtgagtctg cccgccggtt acttcgaccg gctgcccaac
     4321 gaactctcgg gcggagagaa gcaacgggtg gccatagccc gcgccttcgc cgccgacccc
     4381 gacctcatcc tctgcgacga gccgatctcg tccctggacg tctcggtgca gggtgccttg
     4441 atgaacctgc tgatgacgct gcagcaggag aagcgcacct cctacgtctt catctcgcac
     4501 gacctgtccg ccgtgcagca cctctcggac acgatcgccg tgatgtatct ggggagggtc
     4561 atggagatgg gagactcggt ccgggtgctt acgccaccct tccaccccta caccgaggcg
     4621 ctcctctccg ccgtgcccgt ggccgacccg gatgtgacgc agacccccat ccgcctcggt
     4681 ggcaccgtgc cgagcgccat ggacgtgcct gcgggctgcc gcttccaccc gcggtgccca
     4741 cgccgcctcg gcgacatctg ttgcacggag gagccaccgt ggcgtcacgg tgagggcgaa
     4801 caccgcatct actgccacat ccccctggaa gagttggccc acctgcagga gaagactttg
     4861 gtgctcgctg cgggcgaaga gccggccctc cgcgagaagg acgtctgatg ctgcacttca
     4921 tcgcgagacg gctcggcttt gtggtcctga tggtcatact gtcctcgacc atcatcttcg
     4981 ccgccaccca ggtgctcccc ggcgacgtgg ccaccatgat gttgggccgc ttcgcctccg
     5041 agcaggccaa ggagaacctg cgcgaggagc tcggactgaa cgagccgctg ccctcccaat
     5101 acgtccattg gttgggcagt ttcgccaccg gcgactgggg acgctcggtg agcacgcgca
     5161 ccgagatccg cccgctgatc ctgcaacgac tgggcaactc cgccaagttg gccgccgtgg
     5221 ccttcgccat cttcgtgccc ttgggcatac tcctcgggct ggtggcggcg ctacgccgca
     5281 acacctgggt ggatcaggtg atctccgtgg gctcgctggc gttcatcggg ctgccggagt
     5341 tcgtgagtgc ggtcatcctc atcagcatct tctccatcca gctgggatgg ttgccgtcac
     5401 agtcggccat cgatccgaag gcgggcttca tagagtcgtt ccgcttcctc atcctgccgg
     5461 gcgtgacggt ggccctcacc tccctcgcct acatcgtgcg catgacgcgc tcgagcaccg
     5521 tggacgtgct gcagaccgac tacgtgcgga cggccaactt gaagggactc cccccccacc
     5581 gggtgctctt cacccacgtg ctgcgcaact cgctgctccc caccatcacc gtcatcgcca
     5641 tcagcgtggg gtggctcatc gggggactca tcgtcacgga gtcggtgttc ggctatccgg
     5701 ggctgggacg tctggtgcta ttcgcggtgc aaaggcggga catccctctg atccaggcga
     5761 cggtggtggt ggtggtggtg atctacgggg tgtcgaacct tctggcggat atcctctacg
     5821 cggtgctcaa cccgcggatc cggtatacct agctcagcgg gcgcggcgga ccgtcacggg
     5881 gggaccgggg gcgggctgag c
//
LOCUS       DASRMJ010000243         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_346555, whole genome shotgun sequence.
ACCESSION   DASRMJ010000243 DASRMJ010000000
VERSION     DASRMJ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346555"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFD74_06745"
     CDS             complement(<1..475)
                     /locus_tag="VFD74_06745"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZK49279.1"
                     /translation="MWRGRVVPIFLSTTSGPFGWSDIGCLESYRAPMVWWARIPDIWP
                     RMNFLGLSRTSGAIPRRGVKRPYRPSRRRVNILCLEVSGGSSASPSGSPATMSEVPRV
                     RRWEMTMKITAIAGSPRSKSNSTGLLEAALEAARERGHDTKVFTARKLKVAPCIAC"
     gene            435..1502
                     /gene="fbp"
                     /locus_tag="VFD74_06750"
     CDS             435..1502
                     /gene="fbp"
                     /locus_tag="VFD74_06750"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HZK49280.1"
                     /translation="MVDRKIGTTLPRHIYEGQREHPEATGELTGILNQIALAAKIVSR
                     EVNKAGLAEVLGFTGRENVQGEKVRKLDQFANDVFIAAFNHMGHFCIMVSEEVSEPIE
                     IPEKYAGGQYSICFDPLDGSSNIDANVGVGSIFAIFHKVSPGTQGTMEDLLQPGRKLA
                     AAGYVVYGPSTMMVLSTGDGVHGFTLDPSVGEFLESHHDIRIPERGTIYSANEGNYPY
                     WSNGVRRYVSHVKQHDPETGRPYSSRYVGSMVVDVHRTLLYGGIFMYPADTKDPAKPN
                     GKLRLLYECAPMAYLFEQAGGAASTGTGAVLDVVPTKVHQRTPFFGGSHTNVADAERF
                     IAEFDSPSGNGGCAADLPNNR"
     gene            1499..2857
                     /locus_tag="VFD74_06755"
     CDS             1499..2857
                     /locus_tag="VFD74_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZK49281.1"
                     /translation="MSGVEFERARLAGLLAEEEALFARRHPRSLALFERAKAHLLDGV
                     PMNWMTRWASPYPVFVAEAAGPYFTDVDGIRYLDFCLGDTGAMTGHSPSASVEVIADR
                     LRRGITLMLPTEDAILAAEELSSRFGLPYWQVAMTATDANRFVVRLARHITQRPKILV
                     FDWCYHGTVDETFITITDGVPGPRPGNIGPPVDPAVTTKVIQFNDVEALEAALAPGDV
                     ACVLAEPAMTNIGIIHPDPGYHEALREITRRTGTLLVIDETHTICTGPGGYTRAHGLD
                     PDFFVLGKPIGSGIPCAVYGFTSEVADRIKANWDYGNADVGGIGGTLSGSALALAAMR
                     VTLRDVLNEAAYAETIPLAERFNAGVEQVITEFGLPWIAKRLGCRTEYWFRPEAPRNG
                     AEAAAAVDEDLDRYMHLFALNHDILMTPFHNMALIAPGTTEADIDRHTDVFREAVRGL
                     YG"
BASE COUNT          529 a          972 c          916 g          444 t
ORIGIN      
        1 tgcacgcgat acaaggcgcc accttcaact tgcgcgccgt gaacaccttg gtgtcgtgcc
       61 cacgttcgcg agccgcctcg agcgccgctt ccagcaggcc ggtggagttg ctcttcgagc
      121 ggggactccc cgcgatcgca gtaatcttca tcgtcatctc ccatcgtcgc accctcggaa
      181 cctcactcat ggtagcagga gaaccggacg ggctagcgga agaacctccc gatacctcca
      241 ggcataggat gttcactcga cgccgactcg gtcggtacgg tctcttgact ccgcgacgcg
      301 ggatcgcccc ggacgtccgc gacaggccga gaaaattcat gcggggccag atatccggaa
      361 tccgtgccca ccaaaccatc ggcgcgcgat aggattcaag gcatccgatg tccgaccatc
      421 cgaaaggacc gctcgtggta gacagaaaga tcggcacgac cctgccgcgc cacatatacg
      481 agggtcagag ggaacatcct gaggccaccg gtgaactgac cgggatcttg aaccagatag
      541 ccctggccgc gaagatcgtg tcccgcgagg tgaacaaggc gggcctcgcc gaagtccttg
      601 gattcaccgg cagggagaac gtgcagggcg agaaggtgcg caagctcgat cagttcgcca
      661 acgacgtctt catcgccgcg ttcaaccaca tgggccactt ctgcatcatg gtctcggagg
      721 aagtcagcga acccatcgag atccctgaga agtacgccgg cggtcagtat tcgatctgct
      781 tcgacccgct ggacggctcc tccaacatcg acgcgaacgt gggtgtgggc agcatcttcg
      841 cgatcttcca caaggtgagc ccgggcacgc agggcaccat ggaagacctg ctgcaaccgg
      901 gcaggaagct ggctgcggcg ggctacgtgg tctacgggcc cagcaccatg atggtgctga
      961 gcacgggaga cggggtgcac ggattcacgc tcgacccgtc ggtgggcgag ttcctcgagt
     1021 cgcaccacga catccgcatt ccggagaggg gcaccatcta ctccgcgaac gagggcaact
     1081 acccgtattg gtcgaacggc gtccgccggt acgtgagcca cgtgaagcag cacgacccgg
     1141 agacggggcg accgtacagt tccaggtacg tgggctcgat ggtcgtcgac gtgcaccgca
     1201 ccctgctgta tggcggcatc ttcatgtatc ccgccgacac gaaggacccg gcgaagccca
     1261 acggcaagct gcggctgctc tatgagtgcg cccccatggc ctacctcttc gagcaggccg
     1321 gcggcgcggc gagcacgggc acgggagcgg tgctcgatgt cgtgcccacg aaggtacacc
     1381 agcgcacacc cttcttcgga ggctcgcaca cgaacgtcgc cgacgccgaa cggttcatcg
     1441 ccgaattcga cagcccctcc ggcaacggcg gctgcgcggc ggacctcccc aacaatcgat
     1501 gagcggggtg gagttcgagc gtgccaggtt ggcggggctg ctcgccgagg aggaggcact
     1561 cttcgcgcgg cggcatccgc gctcactcgc gctgttcgag agggccaagg cccatctgct
     1621 cgacggcgtt ccgatgaact ggatgacgcg ctgggcctca ccctacccgg tcttcgtggc
     1681 ggaagcggcg gggccctact tcaccgacgt ggacggcatc cgctacctcg atttctgcct
     1741 gggagacacc ggcgccatga ccgggcactc gccgtcggcg tcggtggagg tgatcgcgga
     1801 ccggctgcgg cgcggcatca ccctgatgct acctaccgag gacgccatct tggcggccga
     1861 ggagctctcc tcccgtttcg gcctgcccta ctggcaagtg gccatgaccg ccaccgacgc
     1921 caaccggttc gtggtgcgcc tggcccggca tatcacgcag cgcccgaaga tcctggtctt
     1981 cgactggtgc taccacggca ccgtcgacga gacgttcatc accatcaccg acggtgttcc
     2041 cgggccgcgc ccgggcaaca tcggcccgcc ggtggacccg gcggtaacca ccaaggtgat
     2101 ccagttcaac gacgtggagg ccctagaagc cgccctggcc ccgggcgacg tggcctgcgt
     2161 gctggccgag ccggcgatga ccaacatcgg catcatccac cccgatcccg gctaccacga
     2221 agccttacgg gagatcaccc ggcgcaccgg caccctgctg gtgatcgacg agacacacac
     2281 catctgcacg ggccccggcg gctacacccg ggcgcacggc ctcgaccccg acttcttcgt
     2341 tctgggcaag cccatcggca gcggcatccc gtgcgcggtc tacggcttca cgagcgaggt
     2401 ggccgatcgg atcaaagcga actgggacta cggcaacgcc gacgtgggcg gcatcggcgg
     2461 gaccctttcg ggcagcgcgc tcgcgctggc ggccatgcgg gtcacgctgc gtgacgtact
     2521 caacgaggcg gcctacgccg agaccatccc tctagccgaa agattcaacg cgggcgtgga
     2581 gcaggtcatc accgagttcg gactgccctg gatcgcgaag cggctggggt gccgcaccga
     2641 gtactggttc cgccccgagg ccccgaggaa cggggccgag gcggcggcgg ctgtggacga
     2701 ggatctcgac cgatacatgc acctgttcgc gctgaaccac gacatcctca tgacgccgtt
     2761 ccacaacatg gcgctcatcg ctcccggcac caccgaggcg gacatcgacc ggcacaccga
     2821 tgtgttcagg gaggccgtgc gcggcctgta cgggtaggac c
//
LOCUS       DASRMJ010000244         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_380383, whole genome shotgun sequence.
ACCESSION   DASRMJ010000244 DASRMJ010000000
VERSION     DASRMJ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380383"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            692..1150
                     /locus_tag="VFD74_06760"
     CDS             692..1150
                     /locus_tag="VFD74_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK49282.1"
                     /translation="MTRLDVLDTDRCVGCQCCMFACARRNIDVVSLGLACIKVRSAGG
                     ISRGFRVIVCRACEDPPCAKVCPEDALTLRKGGGVTLIPELCIGCGLCRQACIVGAIH
                     WDRRINKPLICIHCGACAQHCPYGVLGLVRNDRPGALAAVPSRKASHDPG"
     gene            1137..2897
                     /locus_tag="VFD74_06765"
     CDS             1137..2897
                     /locus_tag="VFD74_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZK49283.1"
                     /translation="MIPGDPLANVLYVDLSRRSYRVERREDLFADDIGGAGVGIRLLD
                     ENCPTGADPLGPDNPIVLTVGALTGLFPLASKTVAMFKSPHTGDLGESHGGGRSAVAI
                     RSAGLGAIVITGASSSPVWIAVHAHEVFFRDADTVWGMSSLFTVGRVIRDNEPGAGVR
                     SIMCIGRAGEEMVPYACVTAETYRHFGRLGLGAVFGSKKLKALVVSGTEVIPIADPKA
                     YKRTYKELFAATQSDVMRKYHDLGTAENITPLNAIGGLPTRNLLSGRMPDTERISGEG
                     LAAHYLGRRLACAHCPVGCIHIAALREPYSDDPYFYKTRMISYDYEPIFSLGTLLGIN
                     DPESYLRLMQTVEALGLDCMSAGVVLAWATEAMEHGLITENDTDGVQLVWGEDAPYRA
                     IVRGIVDGKNDFYLALRRGVAFAAQRYGGEDFALSFAGHEMAGYHTGPAAHLGFLFGA
                     RHSHCDNAGYSLDQTALKSGATVPLEEMARSLVAEERWRQVLSSLVVCFFARGIYKPE
                     QTCAALASAGFDVAPADLDALGRRVYRAKYDFKLREGFDPAAVAYPERIFATQSAAGL
                     IDRDEFARAVSVMHAEVLAD"
     gene            complement(3326..3541)
                     /locus_tag="VFD74_06770"
     CDS             complement(3326..3541)
                     /locus_tag="VFD74_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49284.1"
                     /translation="MASATARKGSRHPLRARLSRHCAGVRMTRSQTTPESLAATVVAG
                     YGRVANYPAIPVDGAHRAAEEILSLVG"
BASE COUNT          571 a         1286 c         1198 g          662 t
ORIGIN      
        1 ggatccgcgc gaccaaggcg acgaaatccc cagaaccgac agttctcgag ggtcctgttc
       61 agtcatgtga cggattcggg cgtacaaata tgtgtcgcgg cagccgggcg gtgtcctggc
      121 ggcgtcaccg ggggtctcag ctcaactgac gcccagggag acacggcccg tctgcaatcg
      181 ttttgcaacc caaatgcccg gtccgcgatg ggcttcgttg ggacgccttg ggaaagcggc
      241 cccaaagttt cgctgcaaat cgccacagtt attgtgactt cctgtgacta gctgggtttc
      301 gttgggtcca tctgggcagg ctattgagcc tgcctgataa ggaagaggtc cctggttcga
      361 agccaggtgc gcccaccagt gatttgtagg gaaatcctgc tcccgcctcg ctccgccgac
      421 gtccgtctgc aaccattctg caacccacat gcgtgcgttt ggatgtgagt ggctgggctt
      481 cgttggtgac ggaggtgacg ggcgcgatag cgcgtcatgg cctcaggtgg ctgacggcca
      541 tcccctgcgt aggcacgaat ccactgtcca tgccaacgca aggacagacg agttcctcta
      601 caactgaggt caaagcggcc agctcaacct ccggatgcgt tcttgcgtcg cctcatccgc
      661 acgcgcccct cggaagcgct accatgaagg gatgactcgc cttgacgtgc tcgataccga
      721 tcgttgcgtt ggctgccagt gctgcatgtt cgcctgcgcc cgccgcaaca tcgacgtcgt
      781 cagcctaggc cttgcctgca tcaaggtgcg atccgccggc ggcattagcc gcgggttccg
      841 cgtcatcgtg tgccgcgcct gcgaagaccc gccgtgcgcc aaggtgtgcc cggaggacgc
      901 actcacactt cgcaaaggcg gcggtgtcac cctcatcccc gagctctgca tcggctgcgg
      961 gctctgtcgt caggcctgca tcgtcggggc gatccactgg gaccgccgca tcaacaagcc
     1021 gctcatctgc atccactgcg gcgcctgcgc gcagcactgc ccctacggtg tcctcggcct
     1081 cgtccggaat gatcgtcccg gcgctctcgc ggcggttccc tcgcgaaagg cgagccatga
     1141 tcccgggtga tccgctcgcc aacgtcctgt acgtggacct cagcaggcgc agctaccgtg
     1201 tggagcggcg cgaggatctg ttcgccgacg acatcggcgg tgccggcgta ggcatccgcc
     1261 ttctcgacga gaactgcccc acgggcgccg acccgctcgg ccccgacaat cccatagtcc
     1321 tcaccgtggg agcgctgacc gggctcttcc cgctggcctc caagaccgtg gcgatgttca
     1381 agagtccgca caccggggac ctcggcgaga gccacggcgg ggggcggagc gccgtcgcca
     1441 tccgctccgc ggggctcggc gccatcgtca tcaccggggc cagctcgagc ccggtgtgga
     1501 tcgcggttca cgctcacgag gtcttcttcc gcgacgccga caccgtgtgg ggcatgtcaa
     1561 gcctcttcac ggtgggtcgt gtcatccgcg acaacgagcc cggcgccggg gtacgttcca
     1621 tcatgtgcat cggccgcgcc ggcgaagaga tggtccccta tgcctgcgtc accgccgaga
     1681 cgtatcgcca cttcgggcgc ctcggcctcg gcgccgtgtt cggctcgaag aagctcaagg
     1741 cgctggtcgt ctccggcacc gaggtcatcc cgatagccga tccgaaggcc tacaagcgta
     1801 cctacaagga actcttcgcc gccacgcaga gcgacgtcat gcgcaagtat cacgacctcg
     1861 gcaccgccga aaacatcacc ccgctcaacg ccatcggcgg cctgcccacg cgcaatctgc
     1921 tcagcgggcg catgcccgac acagagcgga tcagcggcga gggtctggca gcgcactacc
     1981 tcggccgccg cttggcctgc gctcactgcc ctgtgggctg catccacatc gccgccctgc
     2041 gcgagcccta ttccgacgac ccctacttct acaagacgcg catgatcagc tacgactacg
     2101 agcccatctt ctcgctgggc acgctgctcg gcatcaacga tcccgagtcc tacctgcgtc
     2161 tcatgcagac cgtcgaagcc ctgggcctcg actgcatgag cgcgggagtc gtgctcgcct
     2221 gggctaccga ggccatggag cacgggctca tcaccgagaa cgacaccgac ggcgtccagc
     2281 tggtgtgggg tgaggacgcc ccgtaccggg ccatagtccg cggcatcgtc gacggcaaga
     2341 acgacttcta cctggcgctg cgcagggggg tcgccttcgc ggcgcagcgg tatggcggcg
     2401 aggacttcgc cctatcgttc gcgggtcacg agatggccgg ctatcacacc ggcccggccg
     2461 ctcacctcgg gttcctcttc ggggcgcggc acagccattg cgacaacgcc ggctactccc
     2521 tcgaccagac cgcgctcaag tcgggcgcga ccgttcccct cgaggagatg gccaggtcac
     2581 tggtcgcgga ggagcgctgg cgccaggtgc tttcgtctct cgtcgtgtgc ttcttcgccc
     2641 gtggcatcta caagcccgag cagacgtgtg cggccctcgc ctcggcgggc ttcgatgtcg
     2701 ctcccgccga cctcgacgct ctcggccggc gcgtctatcg cgccaagtac gacttcaagt
     2761 tgcgcgaggg cttcgacccg gccgcagtcg catacccgga gcgcatcttc gcgactcaat
     2821 cggccgccgg tctcatcgac cgggacgagt tcgcgcgcgc cgtttccgtc atgcacgccg
     2881 aggttctcgc ggactgactg aggtgtcacc actcggcgtc gctgccggct gcgtctcgtt
     2941 cgtcttctac gtctgccgga cgaggtccct ggttcgagtc caggtgcgtc caccggtgat
     3001 ttgcagggaa ctctccatcg caccccttcg ccgagcccgc cggctgcaac cattctgcaa
     3061 cccaagtgcg tgcgcccggg tggggcgcgt tggggttcgt cggcgctagg cgtggccggc
     3121 cgctgggcgc ccgcaaagtg gactcgcagt cgggcttgag cctcttgggg cgagacatct
     3181 cccatcgatg agtggcagcg ggtccgcgct ggaaccacca agctccacgg cgtcgggaca
     3241 aacgccggtc gaaacgtcgc gtggcaggct ggctcctctg cagtcgcggt gtgcctgctc
     3301 gtcttcttgg tgatgcgcag ttgcctcagc cgaccagaga gaggatctcc tccgctgctc
     3361 tgtgcgctcc gtcgacgggg atcgccgggt agttcgccac ccggccgtag ccggccacca
     3421 cggtcgcggc aagactctcg ggtgtcgtct gcgagcgggt catgcgcaca ccggcgcagt
     3481 ggcgcgacaa cctcgcgcgc aacggatgcc gcgaaccttt gcgcgcggtc gcggatgcca
     3541 ctgcctatcc gtcggtcctg ttcttgccgc tgaggcgcag catggcctgg tagagcaacg
     3601 tccccgtggg ggcgggcggg agcgggtcgg tgtggtcggc ggcgaaggcc tgggtcatgt
     3661 aggcgaccag acggcgccag gcgtcggggg ctgtgcccgc ggtagccgcg acgacgc
//
LOCUS       DASRMJ010000245         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_240037, whole genome shotgun sequence.
ACCESSION   DASRMJ010000245 DASRMJ010000000
VERSION     DASRMJ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240037"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VFD74_06775"
     CDS             <1..1048
                     /locus_tag="VFD74_06775"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZK49285.1"
                     /translation="RPLSEIVADLPEGVDRVVLRCLEKLPENRFQSADELLGALVGVG
                     IYIPAKTTGSVPSRLRAAKGRGAAPSPWVPPPGTEAPTPPGVTRSDTSTGDGASPGGG
                     HRRSEVMRERRGRRRAPSGRSLLAVAGVLVILVAGWWLLFGRGADAPNLVGQTLDQAS
                     ATASEAGLKVEVVEQVLDLDRPAGTVLSQEPAVGARAAGGSVRLTVTREPIRVKVTAV
                     DDYDPDGDQEEQPDLLGRLTDGDDATWWRTEQYRNAEFGNLPKTGVGITFKLDAPATV
                     LEVVSPSEGWAAEVQSLSPDGTTTVVGSLTGQARQALIFEQPLDSGRIWITKLAPDEG
                     DRYDVRLAEIRFFR"
     gene            1111..2028
                     /locus_tag="VFD74_06780"
     CDS             1111..2028
                     /locus_tag="VFD74_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZK49286.1"
                     /translation="MNDSLMRVLDAVGRIVLGKEQVIRLSVATVLARGHLLLEDIPGI
                     GKTTLALTLARALGLKFSRIQFTSDLLPADILGSSVFDAKERTFTFRPGPIFHQVVLA
                     DEVNRATPKTQSALLEAMGESQVSVEGTTYRLPEPFFVVATQNPVEQYGTFPLPESQL
                     DRFFMSLRMDYPSRAMEKELLGRLDSKRRIQALAPVIGVQELQDAQERVRNIKASDAL
                     LEYVLEIVWATRRSDKLVVGISPRGAEATVLAAKAWAYLAGRDYCIPEDVQAVAPAVM
                     RHRLVARGDYANLRREQVVEELIRTVPIP"
     gene            2130..>2948
                     /locus_tag="VFD74_06785"
     CDS             2130..>2948
                     /locus_tag="VFD74_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZK49287.1"
                     /translation="MNITRSGWIYIVLTLLVGAAAVNATNNLLYLIASGLLALMSVSG
                     IVAYLALRRLQLEVALPREWFAGQEMPLRMTVVNDRRYLSTFLLSIANAGDRVLLAEI
                     PARGQAEGVLHFALPRRGRHGLGELTVTSAFPFAFFKRGGVLPLHGEVVVYPAPSLPS
                     RDHFLSPSGHEAGTGSQRKGVGGDFWGMREYAPGDPPTRVNWRAWARHGTMVVNEFHE
                     DSTPPLMLTLESVPGPTIEERLSQLTGMILEAGASGRPVGLSLPGTTIQPGVGLS"
BASE COUNT          479 a          991 c         1013 g          465 t
ORIGIN      
        1 gcgtccgttg agtgagatcg ttgcggacct gccggaaggt gtcgaccggg tcgtgctccg
       61 ctgtctagag aagctgcccg agaaccgctt ccagtcggcc gatgagctgc tcggagccct
      121 ggtgggcgtg ggtatctaca tcccggccaa aaccacgggg tcggttcctt cgcggctccg
      181 cgccgcgaag ggacgaggtg cggcgccgag tccctgggtc ccgccccccg gaaccgaagc
      241 gccgacgccc ccaggcgtga cgcgttcgga cacatcgacc ggagatggtg cttcgccggg
      301 aggagggcac aggcggtcgg aagtcatgag ggagcgacgc ggccgcagac gggcaccgtc
      361 gggccgttcc ttgctggccg tcgccggggt cttggtgatc ctggtggcgg gctggtggct
      421 tctgttcggc aggggcgccg acgccccgaa cctggtgggc cagacgctcg accaggccag
      481 tgcgaccgcg tcggaggcgg gactcaaggt cgaggtggtg gaacaggtgc tcgacctcga
      541 ccggccggcg gggaccgtgc tgtcacagga accagccgtc ggcgcgaggg ccgcgggggg
      601 gtcggtgcgg ctcaccgtga cccgcgaacc gatccgtgtg aaggtgaccg ccgtcgacga
      661 ctacgacccc gacggcgacc aggaagaaca gcccgacctg ctgggccggt tgaccgacgg
      721 cgacgacgcc acgtggtggc gcacagagca gtatcgcaac gcggagttcg gcaacctgcc
      781 caagacgggc gtgggcatca ccttcaaact cgacgcaccc gccacggtgc tcgaagtggt
      841 ctcaccctcc gaaggctggg ccgcggaggt gcagagcctc tctccggatg gtacgacgac
      901 ggtggtgggc tcgctcaccg gccaggcacg tcaggccttg atattcgaac aacccctgga
      961 ttccggacgc atctggatca ccaagttggc gcccgacgaa ggcgaccgct acgacgtgcg
     1021 gctggccgag atccgcttct tccggtagat gcgggggcgg cggtcggggt cttgggtgaa
     1081 gcgagcctgc gaacggcgag gtcgagccac gtgaacgatt cactgatgcg cgtattggat
     1141 gccgtgggcc gcatcgtcct gggcaaggag caggtgatca ggctgtcggt ggctaccgtg
     1201 ctggcgcgcg gccacctgtt gctcgaggac atacccggca tcggcaagac cactctggcc
     1261 ctgacgctcg cacgggcgct gggcctgaag ttcagccgca tccagttcac cagtgacctg
     1321 ctgcccgccg acatcctcgg atcctcggtg ttcgacgcga aggagagaac cttcaccttc
     1381 cgccccgggc ccatcttcca ccaggtagtg ctggcggacg aggtgaacag agccactccc
     1441 aagacgcaga gcgcgctcct cgaggcgatg ggtgagagcc aggtgtccgt cgagggcacg
     1501 acctatcgtc tccctgaacc cttcttcgtg gtggccacgc agaaccccgt ggagcagtac
     1561 ggcaccttcc cactgcccga gtcacaactc gaccggttct tcatgtcgct gcgcatggac
     1621 tacccgtctc gagccatgga gaaggagctc ctgggtcggc tcgacagcaa gcggcgcata
     1681 caggccctcg ccccggtgat cggggtgcag gagctgcagg acgcccagga gcgcgtgcgc
     1741 aacatcaagg cctccgacgc cctgctcgag tacgtgctcg agatcgtgtg ggccacgcga
     1801 cggtcggaca aattggtggt gggcatctcg ccgcgcgggg ccgaggccac ggtgctcgcg
     1861 gcgaaggcct gggcatacct cgccggacgc gactactgca tcccggaaga cgtgcaggcg
     1921 gtggcgccgg ctgtcatgcg ccaccgcctg gtagcccgtg gcgactacgc caacctgcgc
     1981 cgcgagcagg tggtcgagga attgatccgc acggtcccca tcccctgacg ccagcctcac
     2041 accgtcctga caccatcccg acaccgccct gacgccgtcc ccgggagcgc cgcatcatac
     2101 gccagcccat ccgattccgc cgaatcaagg tgaacatcac ccgctccggg tggatctaca
     2161 tcgtactcac cctgctggtg ggagcggcgg ccgtgaacgc caccaacaac cttctctacc
     2221 tgatcgcgtc gggactcctg gccttgatgt cggtgtcggg catcgtggcc tatctggcac
     2281 tacgacgact ccaactcgag gtcgcccttc cgcgggagtg gttcgcgggt caagagatgc
     2341 cgctgcggat gacggtggtg aacgatcgcc gatacctctc caccttcctg ctctcgatcg
     2401 ccaacgccgg cgaccgggtg ctcttggctg agatcccggc gcggggtcag gcggaaggcg
     2461 tgctgcactt cgcgctcccc cgccgcggac gccacggcct gggcgagttg accgtgacct
     2521 ccgccttccc cttcgccttc ttcaagcgag gcggcgtgct ccccctgcac ggtgaggtgg
     2581 tcgtgtatcc ggcgccctcc ctgccgagcc gggaccactt cctgagcccc agcgggcacg
     2641 aggccggcac tggatcgcag cgcaagggcg tgggcgggga cttctggggg atgcgcgagt
     2701 atgcgccggg cgacccgccc acccgcgtca actggagagc ctgggcgcgt cacggcacca
     2761 tggtcgtgaa cgagttccac gaagacagca cccctcccct gatgctgacc ctggagagcg
     2821 tccccggtcc gaccatcgaa gagaggctca gccagctcac cgggatgatc ctggaggcgg
     2881 gcgcttcggg gagaccggtg gggttgagtc tcccggggac cacgatccag cccggtgtgg
     2941 ggctgagt
//
LOCUS       DASRMJ010000246         5652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_55521, whole genome shotgun sequence.
ACCESSION   DASRMJ010000246 DASRMJ010000000
VERSION     DASRMJ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55521"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFD74_06790"
     CDS             complement(<1..298)
                     /locus_tag="VFD74_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HZK49288.1"
                     /translation="MIVVAAVIVAWAFILPGGDKRAGSTSTSAAPVPSSTSTSAATTT
                     STLATEEKGTLTYSAQLTGENEIPALSTSASGTLTLTVAPDGSSVRYVLRVSTIT"
     gene            complement(713..1891)
                     /locus_tag="VFD74_06795"
     CDS             complement(713..1891)
                     /locus_tag="VFD74_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HZK49289.1"
                     /translation="MNKRQIRDGVYWMGAVDWERRLFDALIPLPDGTTYNAYLVEGTE
                     KVALLDTVDPAQTDTLLEQLAGVPRIDYLVSHHAEQDHSGSIGMVLEKFPTARVVVTP
                     RAKGMLIDLLALPEDVFMTVEDGDRLPLGGKTLRFVHTPWVHWPETMVTYLEEEGILF
                     SGDFFGSHIASTDLYATDEGRVREAAKRYFAEIMTPFRAHIERNLEKLADYDIQMIAP
                     SHGQIYDRPALIIDAYREWASGPARNLAVLPYVSMHGSTEKMVKHLAAALVDNGVRVE
                     LFDLAVTDIGKLAMALLDAGTVVVGTCTVLAGPHPLAAYATVLANALKPKAQFLSIVG
                     SYGWGGKAVETLAGLIPNLKVEVLDPVLCKGFPRDADLRALDELAATIAQKHKEQQLV
                     "
     gene            complement(2535..3026)
                     /locus_tag="VFD74_06800"
     CDS             complement(2535..3026)
                     /locus_tag="VFD74_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49290.1"
                     /translation="MNRTVMAWVVAAVLAIGGVVPFFTGAASMLYLSTPAQGPQPLVV
                     IYLPFWSAALLVPSSLLVFVLVGRAVGFARFARPLLIMATGAWALDITGFLRVGPSGS
                     LVLTQPAGAMFRQVVRSLRETWSVSGPQIGWIMLVGVFVVAVLVEMMLSRKKAQRVRG
                     RPE"
     gene            complement(3199..3870)
                     /locus_tag="VFD74_06805"
     CDS             complement(3199..3870)
                     /locus_tag="VFD74_06805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZK49291.1"
                     /translation="MNLDKEDIHCHDTRPRVVRVSHEWKGLQMHGEGTGIADNRTQQV
                     RTALTGLMSTIANKPECARAVFRASSRLEEGVRCSVRVRDFDPLVVDEPAELGGGDAG
                     MNPVELVLAALGACQEIMYAAYASVLGMDLGGVEVECKGELDLRGLFGLGESVPPGFT
                     KIRYETKIASLEDEEKIRTLVHLVESRCPVLDILARPVAVSGTTALNGRTLAVREAAK
                     HPAAA"
     gene            complement(4262..5026)
                     /locus_tag="VFD74_06810"
     CDS             complement(4262..5026)
                     /locus_tag="VFD74_06810"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZK49292.1"
                     /translation="MPYRTFEMITLGPVHLRTWGMFIALGILAGAYLAARLAPSRGIP
                     AERLWTLTIVVAVAGVLGSKVLWALQPDLIGETIAHPLRLIAVWQGGLTFIGGLLGAV
                     VAGVLYVRRCGLSVRATADVAAPGLALGIAIGRVGCFLTGLHPGRPTSLPWGIDYLGA
                     VRHPIPLYESALGLVLLGAGLILLKRRAAPGVTALAVALGYLIGRSLLDLLRAGAGEA
                     VSGADPRLLGGLTLTQGVSLVAVPVLAVLVWRARSG"
     gene            complement(5200..5562)
                     /locus_tag="VFD74_06815"
     CDS             complement(5200..5562)
                     /locus_tag="VFD74_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZK49293.1"
                     /translation="MCHLVLLMPVFGLAVFFVLSLPVALVVYLAILGVSILLYKAVID
                     AMSRRVVTGREAMLGTVASVKRVKSGRVVVSLRGELWTAQATGFTDAAPQPGEHVVVS
                     GIRGNTLVVRPLGEGSSG"
BASE COUNT         1107 a         1853 c         1742 g          950 t
ORIGIN      
        1 tggtaatggt gctcaccctg aggacgtagc gcaccgagga gccgtcgggg gccacggtga
       61 gggtcaaggt accgctcgca gacgtggaaa gagcggggat ctcattctcg ccggtcagct
      121 gcgcgctgta ggtaagggtg cccttttcct ccgtagctag agtcgaggtc gtcgtggcgg
      181 cgctcgtcga cgtagaggag ggcaccggcg ctgcggacgt agaggtcgag ccggcccttt
      241 tgtcaccccc aggcaagatg aacgcccacg caactataac cgcggccacg acgatcacga
      301 ccagaatcca agccacacga cgccgtctca cttgtctttg tctttgccgg tacctgccat
      361 tccctgagct gggcgtgagg tatgagggta gtcttggtgt caataatcct cacaagcgat
      421 cgagagggat agatcagcct catggatata ttctgcgaat tctacctaac atccttgcgc
      481 tcggtcgcct tggaggaccg atgcgcaacg ccgaggggcg tcgtaccggg accggccacc
      541 gcgtggctgt tgggctccac ggtgagggcg agcttctcgt cggaggcccg gaaccggaag
      601 gagggcttcg gcgagtgcca tcaagtgcgg aagcttgcgt ccccacgtgt caaacacttg
      661 ccggagccaa ttgcagaaag cacgtaccgg cacggcatgt gagaatctta cctcagacca
      721 actgctgctc cttgtgcttc tgggcaatgg tcgcggcgag ctcgtccagc gccctgaggt
      781 cagcgtctcg ggggaagccc ttgcagagga cggggtccag gacctccact ttgagattgg
      841 ggatcagccc agcgagggtc tccaccgcct tgccgcccca tccatacgag cccacgatcg
      901 agaggaactg ggccttcggc ttcagggcgt tcgcgagcac ggtcgcgtat gccgcgagcg
      961 ggtgcgggcc ggcgagaacc gtgcacgtgc ccacgaccac ggtgccggcg tcgagcaacg
     1021 ccatggccag cttgccgata tcggtaacgg ccagatcgaa cagctcgaca cgcaccccgt
     1081 tgtcgaccag agccgccgcc aggtgtttca ccatcttctc ggtgctcccg tgcatggaga
     1141 cgtagggcag aacggcgaga ttcctcgctg gaccagaggc ccactccctg taagcatcga
     1201 tgatgagtgc tggacgatcg tagatctgcc cgtggctggg cgcgatcatc tggatgtcgt
     1261 agtccgcgag cttctcgaga ttcctctcga tgtgggctcg aaagggcgtc atgatctcgg
     1321 cgaagtagcg cttggcggcc tcgcggacgc gaccttcgtc ggtcgcatac agatcggtgg
     1381 acgctatgtg cgagccgaag aagtcaccac tgaagaggat gccctcctct tcgagatagg
     1441 tgaccatggt ctcgggccag tggacccagg gtgtgtgcac gaacctgagc gtctttcccc
     1501 ccaggggcag cctatcgccg tcctctaccg tcatgaagac atcctcggga agggcgagca
     1561 ggtcgatgag catgcccttg gcccgagggg tgaccaccac tcgcgccgtg gggaacttct
     1621 cgaggaccat gccaatactg ccggaatggt cctgttcggc gtggtgggag acgaggtagt
     1681 cgatcctcgg cactcccgcc agttgctcga gcagggtgtc ggtctgggcc gggtcgacgg
     1741 tatcgagcag ggctaccttt tccgtcccct ccaccaggta ggcgttgtag gtggtgccgt
     1801 cggggagcgg aatgagcgca tcgaacaggc gtcgttccca atctaccgcg cccatccagt
     1861 agacgccgtc gcgaatctgt ctcttgttca cgcgtgtacc tccgccatgg tctgatactg
     1921 atttcctgga ccgatcgctt gccttgcctc cggccgtcgc ggggacgatg tcatcagagg
     1981 tggccgagtc tggccggacg gcttctcttg ggttggtggg tgatgaaccg ctcgtcatgc
     2041 ggccgtttgc ttcagcccta gtgtagacca gtcgacctcg tccgggtgtc cggtcttcga
     2101 gcccctgaca cgacagtacg tctcctcgaa cggcgcaggc ggcggacccg cagcatcaga
     2161 tctccagggc cggggccagc agcggccgct cggcgcttga aactgcctgg cctctaaccc
     2221 ccggccccgc ccgaaatcct cccgacgctt cggcaggccc agggacgctg gtggacgacc
     2281 cccggcatgg atggcgtcac catgacatca tgctggagat gtcgaagcct gcacagcccc
     2341 gacccgcagt ttgactcgga gatggtgcaa tggagtcacc ccatgccatc aggcgccctc
     2401 caaagaaccg ggccaacgca cgtattcgca tggtatgagg tggaaccaga agttccggac
     2461 gacggtccgc ccgtgcccgt gaactcacca gcccgaggcc ggctccccct ttgcatgtct
     2521 cccccgcgcc ggggctactc tggtctccct cggacccgct gcgccttctt ccgtgacagc
     2581 atcatctcaa ctagaacggc gactacgaac acccccacca gcatgatcca cccaatctgg
     2641 ggaccgctga ccgaccaggt ctcgcgcaag ctccggacga cttgtcggaa catggcgccc
     2701 gcgggctgcg tcagcaccag gctgccgctc ggtcccacac gcaagaatcc cgtgatgtct
     2761 agggcccagg ctcccgtcgc catgatgagc agcggccgcg caaaccgggc aaacccgaca
     2821 gcccgcccta cgagcacgaa gacgagaagg gacgacggca caaggagcgc cgccgaccag
     2881 aagggcagat agatcacgac aagtggctgt ggcccctggg cgggcgtcga gaggtacagc
     2941 atactggcgg ccccggtgaa gaacggcacg acgccaccga tcgcgaggac tgcggcgacc
     3001 acccatgcca tcacggttcg gttcaaccgg tccgcctttg tcgacatgca gccgcagact
     3061 caggatatcg gagagtcact ccgggggaca gtcgacgtct gactggatca cgccgccgtc
     3121 ctgtgaatcg tggagtcagc tgaccttgga tgcgaccggg agttcgtgga ttaacgaccg
     3181 cccgcaccgg cactcctctc atgcagccgc gggatgcttc gccgcctcgc ggacggccag
     3241 cgttcgaccg ttgagagcgg tggttccgct caccgcgacc ggccgcgcca agatatcgag
     3301 taccgggcac cgcgattcca ccaagtggac cagcgtccgt atcttctcct catcctcaag
     3361 gcttgcaatc ttcgtctcat aacgaatctt ggtaaacccc ggtggcaccg attcgcccag
     3421 gccgaacaac cccctcaggt cgagttcgcc tttgcactct acctccaccc cgccaagatc
     3481 catccccaac accgaggcgt aggccgcata catgatctcc tggcacgcac ccagagcagc
     3541 cagcaccagt tcgaccggat tcatcccggc gtctccgcca cccagctccg caggctcgtc
     3601 gacaacaaga gggtcgaagt cccgcacccg aacgctgcag cgcactccct cctccagacg
     3661 acttgacgcc cgaaagaccg ctctggcgca ctccggcttg ttcgcaatcg tggacatcaa
     3721 cccggtcagt gcagtccgta cttgttgggt cctgttgtcg gcaattcctg ttccctcacc
     3781 gtgcatctgc aaccccttcc actcatggct aacgcgaaca acccttggtc gcgtatcatg
     3841 acaatgtata tcctctttgt caagattcac atacacatcc tctttgctcg cccgtagagg
     3901 ggtgcatttg ggccggctcg ggaagatctc gcgcaacaga gggtcgggct gatcggggag
     3961 cactggcagt gagtgggctc ccccgtgcaa actcaagcgc cacgagcgag cgctccctgg
     4021 atcttggcgg gcagtatgct cgtagtagga cggagcatgg agaatacgtg gctaagaaga
     4081 ccggacggat tccaccctgc acgaaaggct cgccaccaat aagcacttct agttcttcct
     4141 gcgggctttc aggatccggc cgggcccaag aaatgccaag ctatggcctc cgaggctctt
     4201 ggcttcgatt cgtaggcaga cacgggtgtg gaggaactcg agggaggcac acggacgaag
     4261 ctcaaccaga tcgcgccctc cacaccaata ccgccaacac gggcaccgcg accagcgaca
     4321 caccttgcgt caacgtgagc ccgccgagaa gtcggggatc ggctccggag accgcctctc
     4381 ccgcgcccgc ccgcaggagg tcgagcagcg agcgcccgat caagtatcca agggccaccg
     4441 ccaacgctgt gacgccagga gcagcacgtc gcttcagcag gatgaggccg gcaccgagca
     4501 gcaccaggcc aagcgcggac tcgtagagcg ggatcggatg gcgaactgcc cccaggtagt
     4561 cgatgcccca cggcagcgaa gtcggccgtc cggggtgaag ccctgtcaga aagcacccga
     4621 ccctaccgat ggcgatgccg agggccagcc cgggtgcagc cacgtcagcc gtggcacgga
     4681 cggaaagtcc gcatctgcga acgtacaaga ccccagcaac caccgcaccg agcagcccgc
     4741 cgatgaaggt gagtccccct tgccacacgg cgatgagccg cagggggtgg gcgatagtct
     4801 caccgatgag gtctggctgg agcgcccaga gcactttact cccgagcact ccggccacag
     4861 ccaccacgat cgtgagtgtc cagagcctct cggcgggaat cccgcggctg ggagcaaggc
     4921 gggccgccag gtaggcacca gcaaggatgc cgagtgctat gaacatgccc cacgtgcgta
     4981 gatgaacggg gcccagcgtg atcatctcaa aggtgcggta cggcacggtg ctgtctcccc
     5041 tatcgaaggc ggctggtctg gagtccgctg cccacaggcc agcggaacgc ggaagggcag
     5101 gtcaagcaag cgcaaagatg cgcgcgagga cacggacctt caccctggga agatccgcag
     5161 ccaagagcac gcaagagctc gcccgacgca ctcgagaact cagcccgatg agccctcccc
     5221 gagcggtctc acgacgaggg tgttgccgcg gatgccgctg accaccacgt gttcacccgg
     5281 ctgcggcgcg gcatccgtga atccggtggc ttgggcagtc cacagttcgc cgcgtagaga
     5341 cacaacaacc cgtccggact tcacgcgttt cacgctggcc acggtgccta gcatcgcctc
     5401 gcgtcccgtg accacccggc ggctcatcgc atcgatcacg gccttgtaga gcaggatgct
     5461 cactcccagg atggcgaggt aaacaacgag tgcgaccggc aacgaaagga cgaagaacac
     5521 cgccaaaccg aagaccggca taagaagaac gagatggcac atctagcggg agctccagcc
     5581 ttcggcccag atctcttcac cctcgagcgc gagcttgagt gtgggcagag gactgggcgg
     5641 ggggccggcg ag
//
LOCUS       DASRMJ010000247         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_380551, whole genome shotgun sequence.
ACCESSION   DASRMJ010000247 DASRMJ010000000
VERSION     DASRMJ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380551"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFD74_06820"
     CDS             <1..633
                     /locus_tag="VFD74_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HZK49294.1"
                     /translation="AALGLTAAVAYRTLFSPQPGKTTDGGAVTTGTTATTSSTDGDAP
                     GTTTQTTSAGQPTTSVTGTQAGRLLKTSSWMASSTLPAEGGNDYGAANVADGDLGTCW
                     SEGIDGQGAGEWVRLAFDKPTVLTGIEIANGYQKDDRRFSGNPRVAVLRIEYSTGSAQ
                     TVQLHDSMGFQTVTPSPTPTQWVKFVVESTYPGELWQDTSISEIRLYESQ"
     gene            complement(673..873)
                     /gene="dmpI"
                     /locus_tag="VFD74_06825"
     CDS             complement(673..873)
                     /gene="dmpI"
                     /locus_tag="VFD74_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase DmpI"
                     /protein_id="HZK49295.1"
                     /translation="MPIITVEGPPMRDMDKRRVFVKSLTEAAVEAYGLPASAMVVVLH
                     ENPLECVASGGELISDRRVSAS"
     gene            complement(924..2198)
                     /locus_tag="VFD74_06830"
     CDS             complement(924..2198)
                     /locus_tag="VFD74_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK49296.1"
                     /translation="MEHTIATRTDGPTASGDRIFSKDFNLALLSNALAFGSFYLLLAT
                     LPLFVKHLGGSDTGVGVVMGSFAVTAVILRPFVGRYSDVSGRKRLMIWGALLLVVVSL
                     LYGRATGVGWLILLRSAYGVGWAAFGTSAWAMASDIAPARRRGEAMGYFGIAMNLSMA
                     VGPAAGVLLVQRWGYGALFWAAAALAGAAALASLGVSSDHVTRSSRAAAPAGTRAVLL
                     RSALFPSAVLICMALTYASVITFLPVYADVRGLGNPGIFFSVYAVFLIVSRGPLGKAS
                     DRFGRGQVVAPGMAMIAASMLLLAWVSTFAGMLVVAALYGLGVGAVQPVLMAWTVDRA
                     RSSERGAAMGTFTAAMDVGVGLGSLTWGIVADSAGFGAVYVGSAAVGMVGLVMLVAGG
                     GLRSAAVGPIHSLRGDGGVADGAAASAVSRTR"
     gene            complement(2295..2504)
                     /locus_tag="VFD74_06835"
     CDS             complement(2295..2504)
                     /locus_tag="VFD74_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49297.1"
                     /translation="MARIKLFAEFEAVFDVDLPGVDSETQRHEVEQHRAEVLAVLQDR
                     LREAFGGDNLKRIEYFEFDVERPSD"
     gene            2720..3280
                     /locus_tag="VFD74_06840"
     CDS             2720..3280
                     /locus_tag="VFD74_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZK49298.1"
                     /translation="MRPTEDLVREHGDILAMIQVVREMVRRLDAGTAVDPGHIEKAIY
                     FIRHYADKYHHAKEEDLLFPAMHDAGVPREGGPIAVMLAEHDEGRAYVRNAAEALERY
                     RAGDGAAAQVVAANLRNYGGLLEQHISKENQVLYPIADAHIGQTGQTELRWSFDHVNA
                     KPEMVEECARCKAILMELDPIYLDED"
     gene            complement(3414..>3941)
                     /locus_tag="VFD74_06845"
     CDS             complement(3414..>3941)
                     /locus_tag="VFD74_06845"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZK49299.1"
                     /translation="NLLRLTFGEWGETLARRARGQDARSVTTHEAVKSISRETTFEED
                     TTDSARLESTLLHLTEDVCRRLRRQRLEARTVTVKIRYSDFVTHTRSRTLRRPLDVDE
                     VFFEEVLSLFREGRQRRYRIRLVGVGLSNLVARSWQDDLFDQDLPLLRDLDLKLDTIR
                     EKYGRDAVRRGAAEE"
BASE COUNT          746 a         1402 c         1242 g          551 t
ORIGIN      
        1 gccgcgctcg gtctcacggc cgccgtcgcc tatcgcacgc tcttctcccc acagccggga
       61 aagacgaccg acgggggcgc cgtcaccacc ggcacgacgg ccacgacgtc gagcaccgat
      121 ggcgacgccc ccgggaccac cacccagacc acgtcggccg gccaaccgac cacgagcgtc
      181 accggcaccc aggccggtcg tctgctgaag acgtcctcgt ggatggcctc ctccaccctc
      241 ccagccgaag gcggcaacga ctacggggcg gccaacgtcg ccgacgggga cctcggcacg
      301 tgctggagcg aaggcatcga cgggcagggc gcaggcgagt gggtccgcct tgcgttcgac
      361 aagccgacgg tcctcaccgg catcgagatc gcgaacggct accagaagga cgatcgtcgc
      421 ttctcgggga acccgcgtgt ggccgtgctg cgcatcgagt actccacggg ctcggctcag
      481 acggtccagc tccacgactc gatgggtttc cagaccgtga cgccctcgcc cacgcccacg
      541 cagtgggtga agttcgtggt ggagtccacg tatccgggag agttgtggca ggacacctcg
      601 atctccgaga tccgtctgta cgagtcgcag taggaggcgt actaggggtc ggacgtccgg
      661 ccggtccccc ctctacgagg cggacacccg cctgtcggag atgagctctc ccccgcttgc
      721 gacacactcg agcgggttct cgtgcaggac cacgaccatg gcactggccg gtagtccgta
      781 cgcttcgacc gccgcttcgg tcagcgactt cacgaacacc ctccgcttgt ccatgtcgcg
      841 catcgggggt ccctcgacgg tgatgatcgg catcgcctgc tcctctcctg gagcctcttg
      901 ggctccgtcg ctcctgggtg catctatcgc gtgcgtgaca cggctgacgc cgcggccccg
      961 tctgccactc cgccgtcccc ccgcaaagag tggatggggc cgaccgccgc ggaccgaagg
     1021 cctcctccgg ccaccagcat gaccagaccg accataccga ccgccgcgga gccaacgtag
     1081 acggccccga acccggcaga gtctgccacg atcccccagg tcaggctacc cagacccacg
     1141 cccacatcca tcgccgcggt gaaggtgccc atcgcggccc cgcgctccga cgaccgggcg
     1201 cggtcgacgg tccacgccat gaggaccggc tgcaccgcgc cgactcccag accgtagaga
     1261 gcggcgacca cgagcatgcc cgcaaacgtg gagacccacg ccagcaagag catcgacgcg
     1321 gcgatcatgg ccatgccggg agcgaccacc tgcccccggc cgaagcgatc ggaagccttg
     1381 ccgagcggcc ccctcgacac gatcaggaac accgcgtaca ccgagaagaa aatgcccgga
     1441 ttgccgaggc cacgcacatc ggcatacacc ggcaggaagg tgatcacgga ggcgtacgtg
     1501 agcgccatac agatcagcac cgcagatgga aacagcgccg accgcagcag cacggcgcgg
     1561 gtgccggccg gggcggcggc acgactcgaa cgcgtgacgt ggtcggacga gacccccaac
     1621 gaggccagcg cggcggcgcc cgccagcgcc gccgccgccc agaagagggc tccgtacccc
     1681 cacctctgca cgaggaggac gccggccgcg ggccccaccg ccatggaaag gttcatggct
     1741 atgccgaagt agcccatggc ttcgccccgc cggcgggcgg gtgcgatgtc cgaagccatc
     1801 gcccaggccg aagtgccgaa cgcggcccag cccaccccgt aggccgaccg cagaaggatc
     1861 aaccacccga cgccggtcgc ccggccgtag aggagcgaga ccaccaccaa caacaacgcg
     1921 ccccagatca tcaaccgctt gcgaccgctc acgtcggagt agcggcccac gaacggccgc
     1981 aggatgaccg cggtgaccgc gaacgaaccc atgacgacgc ccacccccgt gtcgctgccc
     2041 ccgaggtgct tcacgaacag aggtagtgtc gcgaggagta gatagaagct gccgaacgcc
     2101 aacgcgttgg acagaagggc gaggttgaaa tctttggaga agatgcggtc tccggatgcg
     2161 gttggtccgt cggtccgcgt ggcgatggtg tgttccatcc cggaacgata gcacggcgca
     2221 gctgttccag ggtgccgctt atatagagaa gtcggcgggc aggtcgcgca gacgaggact
     2281 gcacgatcgg cggatcagtc cgacggtctc tccacgtcga actcgaagta ctctatgcgc
     2341 ttgagattgt cgccgccgaa ggcttcgcga agacgatcct ggaggaccgc gagcacctcg
     2401 gcccgatgct gctccacttc gtgccgctgg gtctccgagt cgacgccggg gaggtccacg
     2461 tcgaaaaccg cctcgaactc cgcgaacaac ttgatcctcg ccatctcagc ccctctcttc
     2521 gcaggcgact cgccgcgccc gtcacgcgcg tcatacccac cctgcgacga ccggctaaca
     2581 tcgccgccga cacctggcac ccagtgcctg gcacctcgat caatccaggt ttacactatt
     2641 tcgcccgtgg taatacactc atcggcactc gcacagggcg atgccgccgg actgaaccag
     2701 aagacctgga ggcaagacgg tgagacccac ggaagacctg gtgcgcgaac acggtgacat
     2761 cctggccatg atccaggtgg tgagagagat ggtgcgtcgg ctggacgccg ggacggccgt
     2821 cgaccccggc cacatcgaga aggccatcta cttcatacgc cactacgccg acaagtacca
     2881 ccacgcaaag gaagaggacc tactcttccc cgcgatgcac gacgccggcg tgcccaggga
     2941 aggcggtccg atcgccgtga tgctcgcgga acacgacgaa gggcgggcat atgtgcgcaa
     3001 cgcagcggaa gccctcgaga gataccgcgc gggagacggc gctgcagctc aggtcgtggc
     3061 cgccaacctg cgcaactacg gaggccttct cgaacagcac atctcgaagg agaaccaggt
     3121 gctctacccg atcgcagatg cccacatcgg gcagacgggt cagacggagt tgcggtggtc
     3181 gttcgaccat gtcaacgcca agccggagat ggtcgaggag tgtgcccgct gcaaggccat
     3241 cctcatggaa ctcgacccga tctacctgga cgaggactga aagacggcgc tgcggccttc
     3301 gcctccgcgg aggcgccgac tacggccccg gcctcgccta gttgccaccc gcttcccgag
     3361 cccacggccg gccacagccg agtgacggct cccgtcggcc tccgctcgga gccctactcc
     3421 tcggccgccc cgcgtctcac cgcgtcgcgt ccgtacttct cgcggatggt gtcgagtttc
     3481 agatcgaggt cgcgcaggag cggcaggtct tggtcgaaca ggtcgtcttg ccaggagcgc
     3541 gccaccagat tggacaggcc caccccgacg agccggatgc ggtagcgccg ctgccgaccc
     3601 tcccgaaaga gggacaggac ctcctcgaag aagacttcgt ccacgtccag agggcgacgg
     3661 agcgtgcgcg accgcgtgtg cgtgacgaaa tcggagtagc ggatcttcac cgtgaccgtg
     3721 cgggcttcaa gccgttgacg ccgtagtctc cgacacacgt cctcggtcaa gtggagcagt
     3781 gtcgattcga ggcgcgcact atccgtggtg tcttcctcga aggtggtctc ccgcgagatg
     3841 gacttcacgg cttcgtgcgt agtcaccgaa cgcgcatctt gaccgcgcgc ccgccgcgcc
     3901 agcgtctcgc cccactcacc gaaggtcagg cggagcagat t
//
LOCUS       DASRMJ010000248         6934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_22320, whole genome shotgun sequence.
ACCESSION   DASRMJ010000248 DASRMJ010000000
VERSION     DASRMJ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22320"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFD74_06850"
     CDS             <1..589
                     /locus_tag="VFD74_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZK49300.1"
                     /translation="VAFERGSMREVISDAETGFLVGSVEEAVAVIGAAGTGGGAGTDG
                     PLAALDRRACRRWVEEHFSVERMVDGYLAVYQRVLEQSRREDHRPWGYYKVLEDEPAH
                     KTKRIVVLPGARLSLQRHRRRSEHWHVVSGRAVVTRDDEELPLGAGESVDIPMGAAHR
                     MANPGTEPLTFIEVQRGDYFGEDDIERLEDDFGRS"
     gene            592..1524
                     /locus_tag="VFD74_06855"
     CDS             592..1524
                     /locus_tag="VFD74_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HZK49301.1"
                     /translation="MIRRHPGNPILTKADVPYPVATVHNAGAVKYDGRYLLLFRSHRR
                     NGRSIIGLAESDDGFRFTVRPEPFLVPATNGVFAEYEEWGIEDVRVCPMEGEYLLTYS
                     AYSRHGVRVALARTADFRRVERVALITPADSRNVVIFPERIDGRYVRLDRPHSEISPW
                     SIWITYSPDLVHWGDSRVVMQPVPYHWDEQKIGPGATPIKTPRGWLHIYHGVYPTMAG
                     AVYRLGVALHDLADPARVLGVSDEWILEPEDPWERVGYVPNVVFTCGAIPEDDGTVKI
                     YWGGADTVMCAGTAVIDELVDLCLDAPRASRGDV"
     gene            1517..1891
                     /locus_tag="VFD74_06860"
     CDS             1517..1891
                     /locus_tag="VFD74_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK49302.1"
                     /translation="MYESAGLQRQPRKNRSTATLPEHALSGCAASGAEIEVVHLCDHA
                     YQGCISCFACKKIGGKSYGRCNVRDGLSPLLDRAAQADILILGSPVCSHTETGEIRSF
                     VERLLFPYLTYTPDRASLFPSR"
     gene            complement(1973..2797)
                     /locus_tag="VFD74_06865"
     CDS             complement(1973..2797)
                     /locus_tag="VFD74_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZK49303.1"
                     /translation="MRYQREFIGEALGTFVLVLFGCGSVAVSVLFNAYQGLMQIALAW
                     GIGVALAIYLTRHLSCAHLNPAVSLAMVLGGRMHRRKLPVYLVAQFVGAVVAGLVLYV
                     LFSSSIAAFESAHGIVRGTAESIRTAAMFGEFYPAPDHTAVVSLPLAMGAEAMGTFLL
                     VLMIFALTEGCNVGRPDDALAPVFIGLTVTSIIALIAPLTQAGLNPARDLGPRLVAWL
                     AGWGAAAFPDRVGGFLYVYVLAPIVGGLLAMVFFVRVLEPMMRRQPAPSCCGDDEE"
     gene            2950..4506
                     /gene="thrC"
                     /locus_tag="VFD74_06870"
     CDS             2950..4506
                     /gene="thrC"
                     /locus_tag="VFD74_06870"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZK49304.1"
                     /translation="MTRISYRETRALDPTPRSFTDVVLKGIAEGGGLYVPDRLPELDL
                     KTVLSLAALPYHGRAFEVFRRFGLDVPEARQAELLAEAYAANFDHPAVAPVVSMGAAA
                     PGMHMLELWHGPTSAFKDMALQCMPLFFSEAVAMRRTAGADVPDFLVLVATSGDTGKA
                     ALEGFADRPSTRIAVLFPAEGVSEVQRRQMTTQHGANVAVYGVNGNFDDCQNAVKAAF
                     SDPGFNTLLRRDHHTELSSANSINWGRLLPQIVYYASAYADVAAAGGLEPGAPLDVCV
                     PTGNFGNILAALYAKRMGVPIGRLICASNENDVLANFIATGTYSIKDRPFVTTPSPSM
                     DILISSNLERALYEVCRDPERIRGWMAGLATEGAFSVDPGTRAVVQELFVGESVGNDE
                     SLEVIRRVLADTGRLLDPHTAVAWAAAARQKGDAPMLVVSTAHWAKFGTDVYKALRGL
                     PHRDPLPADTAGMSEFQLLDQVLRLAPGEKIPAGLAGLETAPVRFTTTIGRTRDAVVG
                     AVLGWLAGGA"
     gene            4511..5356
                     /locus_tag="VFD74_06875"
     CDS             4511..5356
                     /locus_tag="VFD74_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZK49305.1"
                     /translation="MTVRIVTDSSSCLPPGWAEENGVSVIPQGIVVDGTAYRENVDLS
                     GDEFYRLLEWGSTATSSQPRPDAFVTAYRAALDAGDEMVSVHIGSDLSGTLDAAYSHA
                     AGLRTPDSPARIHLVDSCSAGLGLGFLVVEAARLAAEGAEALSIVTALEAMVPKIRVY
                     FMLHTMTYLVRGGRVGRAAGLAASLLKLRPILTINDGEIAPAARTRTPHAAAAQLWAL
                     IDASARRGVRYAGFHYGLNRDEVDGWRQEFTRRYGLEAWLTQLGPAVGAHSGPRVLGV
                     VLVEE"
     gene            5551..5787
                     /locus_tag="VFD74_06880"
     CDS             5551..5787
                     /locus_tag="VFD74_06880"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HZK49306.1"
                     /translation="MPVSLSIKNVPEDVAAQLRDRAKAHRRSLQGELLAIVEQAVAAP
                     AELTPAGLKAYVDSLGLLTDDDSVGIIRTDRDGR"
     gene            5774..6226
                     /locus_tag="VFD74_06885"
     CDS             5774..6226
                     /locus_tag="VFD74_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZK49307.1"
                     /translation="MTAADEGIAPSGDSAPGTYPPAGSQPLVVVDASVFGALVFSESR
                     SREAADLLAEATLISPGLVRYEMANIGRTKIMRNPESGALITRAFRRWLELPVRLVTP
                     DFGGVLSLAVAEQLSAYDAAYVYVAAALGAPLLTFDEQLGATWRRISG"
     gene            complement(6216..>6934)
                     /locus_tag="VFD74_06890"
     CDS             complement(6216..>6934)
                     /locus_tag="VFD74_06890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZK49308.1"
                     /translation="GERLVDVGCGWGGMLLYAAREYGVEGVGYTLSSEQHRFANERLR
                     AEGLYPRVRVELLDYREAEGEFDKFVSIGMFEHVGKEYYGEFFSKVGELLAPGAVGVL
                     HTIGTQCEADVDPWIGKYIFPGGYLPTLGQICESMGQTDLVATDVENLRLHYARTLDA
                     WAERFESCVDEVRRRFSESFVRMWRFYLHASAAGFKYGDARVYQVTFTNGLRSDLPLT
                     RAHLYEPKTRSRRDAQPVSR"
BASE COUNT         1155 a         2420 c         2276 g         1083 t
ORIGIN      
        1 ggtggccttc gagcggggga gcatgcgcga agtcatctcc gacgccgaga ccgggttcct
       61 ggtggggtcg gtggaggagg cggtggccgt catcggagcg gcgggcacgg gcggaggcgc
      121 gggcaccgat ggccctctgg cggccctcga ccgccgggcc tgccgccgct gggtcgagga
      181 acacttcagc gtcgagcgga tggtcgacgg ctacctggcg gtctaccaac gggtgctgga
      241 gcagtctcgg cgggaggacc accggccctg gggctactac aaggtgctcg aagacgagcc
      301 cgcccacaag accaagcgca tcgtcgtgct gccgggcgcg cgcctgagtc tccagcgcca
      361 ccgacgccgg tccgagcact ggcacgtggt gtcgggccgg gccgtggtga cccgcgacga
      421 tgaggagctg cccctcggcg ccggggagtc cgtcgacata ccgatggggg cggctcaccg
      481 catggccaac ccgggtacgg aacccctcac cttcatcgaa gtgcaacgag gggactactt
      541 cggcgaagac gacatcgagc gcctggaaga cgacttcggg cgatcgtgag tatgatccgc
      601 cgccaccccg gcaaccccat cctcaccaag gccgacgtac cctacccggt ggcgacggta
      661 cacaacgcgg gggcggtcaa gtacgacgga aggtacctgc tgctcttccg ttcacaccgc
      721 cgcaacggcc gatcgatcat cggcctggcc gaaagcgacg acggcttccg cttcaccgtg
      781 cgcccagagc cctttctcgt gcctgccacg aacggcgtct tcgccgagta cgaagagtgg
      841 ggcatcgagg acgtgcgcgt gtgcccgatg gagggggaat acctgctcac ctacagcgcc
      901 tactcgcgtc acggcgtgcg ggtcgcgttg gcccgcaccg ccgacttccg cagggtagag
      961 cgcgtggcgc tcatcacccc ggccgacagc cgcaacgtgg tgatcttccc ggagcgcatc
     1021 gacggaaggt acgtgcgact ggaccggccg cattcggaga tctctccctg gagcatctgg
     1081 atcacctact cccccgacct ggtccattgg ggcgactcgc gggtggtgat gcagccggtc
     1141 ccgtaccact gggacgagca gaagatcggc cccggggcga cgcccatcaa gacgccgcgg
     1201 ggctggctcc atatctatca cggcgtctac ccgactatgg ctggcgccgt ttaccgactg
     1261 ggtgtggccc tccacgacct cgccgacccg gcccgcgtgc tgggcgtgtc cgacgagtgg
     1321 atcctcgaac ctgaggaccc ctgggagcgc gtgggctacg taccgaacgt ggtgttcacc
     1381 tgcggagcca tacccgagga cgacggcacc gtgaagatct actggggcgg tgccgacact
     1441 gtgatgtgcg caggcacggc tgtcatcgac gagttagtgg acttgtgtct tgacgccccg
     1501 cgggcctcga ggggggatgt atgagagcgc tggccttcaa cggcagcccc ggaagaatcg
     1561 gagcaccgcg accctgccgg agcacgctct gagcggctgc gcggcgagcg gcgcggagat
     1621 cgaggtggtg cacctctgcg accatgccta ccaaggttgc atcagttgct tcgcctgtaa
     1681 gaagatcggc gggaagagct acggtcggtg taacgtgcgg gacgggctca gcccactcct
     1741 cgaccgggcg gcccaggccg acatcttgat attgggatcg cccgtctgct ctcacaccga
     1801 gacgggcgag atacggtcgt tcgtggagcg tctgctcttt ccgtacttga cgtacacgcc
     1861 cgaccgtgcc tccctcttcc ctagcagatg agatagcgca cgtcgacggc gagcagcggg
     1921 taggacacat cgaccgccgt ggccggtacg tctggcagcc gatccggtaa cgctactcct
     1981 cgtcgtcccc gcagcagctc ggggcaggct gacgtctcat catcggctcc agcacccgaa
     2041 cgaagaagac catcgcgagc agaccgccca ctatgggggc gaggacgtac acgtagagga
     2101 accctcccac gcggtcgggg aacgccgcgg cgccccaccc cgcgagccag gccaccaacc
     2161 gggggcccag atcgcgtgcc gggttgagcc cggcctgcgt caacggcgcg atgagggcga
     2221 tgatcgaagt gaccgtcaag ccgatgaaca ccggagccag ggcgtcgtcc ggacgcccga
     2281 cgttgcatcc ttcggtcagg gcgaagatca tgagtacgag caagaatgtg cccatggcct
     2341 ccgcccccat cgcaagtggc agggagacga cagccgtgtg atccggcgca gggtagaact
     2401 cgccgaacat cgcggcggtc ctgatcgact ccgcggtgcc tcgcacaatc ccgtgagcgc
     2461 tctcgaatgc cgcgatcgaa gacgagaaga gcacgtacaa caccaggccc gccacgacgg
     2521 cgcccacgaa ctgggccacg aggtacaccg gcaacttgcg gcggtgcatc cgcccgccaa
     2581 gaaccatggc cagactcacc gcggggttga ggtgggcaca cgacaagtgc cgcgtgaggt
     2641 agatcgcgag agccaccccg atgccccacg ccaacgcgat ctgcatcagc ccctggtagg
     2701 cattgaatag caccgagacc gcgacggacc cgcatccgaa taacaccagc acgaaggtgc
     2761 cgagcgcttc tccgatgaac tccctttggt atctcatccg tccgctcgca agcaatgccg
     2821 caccgttccc aagatggccc cgctcaggag gatacacccc cgcgccccca ccggagccgc
     2881 cggcgcatcg gatgcgctgg cggcctgcta ccattgagtc tcggccacgt ctgaggagca
     2941 cgtccgacca tgacccgcat ctcataccgc gaaacccgcg ctctcgaccc cacgccgcgc
     3001 agcttcaccg acgtcgtgct caagggcatc gccgaaggcg gcggactcta cgtgcccgac
     3061 cggctgccgg aactcgacct caagacggtg ctgagcctgg ccgccctccc ctaccacggg
     3121 cgggccttcg aggtgttccg gcgcttcggg ctcgacgtgc ccgaagcccg gcaggcggag
     3181 ctgctcgccg aggcctacgc cgccaacttc gaccatccgg cggtagcccc ggtcgtgtcc
     3241 atgggggctg cggctcccgg tatgcacatg ctcgaactct ggcacggtcc cacctccgcc
     3301 ttcaaggaca tggccctgca gtgcatgccc ctcttcttct cggaggcggt ggctatgcgc
     3361 cgcaccgccg gcgccgacgt gcccgacttc ctcgttctgg tggccacctc tggcgacacc
     3421 gggaaggccg ccctcgaggg cttcgccgac cggcccagca cccgcatcgc cgtgctgttc
     3481 ccggcggaag gcgtgtcgga ggtgcagcgc cgccagatga ccacccagca cggagcgaac
     3541 gtggccgtat acggcgtgaa cggcaacttc gacgactgcc agaacgcggt caaggccgcc
     3601 ttctccgacc ccggcttcaa caccctgctc cgccgcgacc atcacaccga actctcatct
     3661 gccaactcca tcaactgggg gcggctgctg ccgcagatcg tctactacgc gagcgcctac
     3721 gccgacgtgg ccgccgccgg gggcctcgag ccgggcgcgc cccttgacgt gtgcgtgccc
     3781 acgggcaact tcgggaacat cctggccgcc ctctacgcca agcgcatggg cgtccccatc
     3841 ggccggctca tctgcgcgtc gaacgagaac gacgtgctgg ccaacttcat agccaccggc
     3901 acctactcca tcaaggaccg gcccttcgtc accacgccct cgccgtcgat ggacatcctc
     3961 atctcgtcga atctggagcg ggcactgtat gaggtgtgcc gcgatccgga gcgcatccgg
     4021 ggctggatgg ccggtctcgc cacggagggc gcattttcgg tggacccggg cacacgcgcc
     4081 gttgtgcagg agctcttcgt tggcgagtcc gtgggaaacg acgagagtct cgaggtgatc
     4141 cgtcgtgtgc tggccgacac gggccgactt ctcgacccgc acaccgccgt ggcttgggcg
     4201 gcagccgcgc ggcagaaggg cgacgcaccc atgctggtgg tatccaccgc ccactgggcc
     4261 aagttcggaa cggacgtcta caaggctctg cgtggcctcc cccaccgtga cccgctgcct
     4321 gccgacaccg ccggcatgtc cgagttccag ctgctcgacc aggtgctgcg cctggccccg
     4381 ggcgagaaga tcccggcggg actagccggc ctggagaccg cgcccgtgcg cttcaccaca
     4441 accatcggcc gcacccggga cgcggtggta ggtgcggtgc ttgggtggct ggcggggggg
     4501 gcgtagccgc atgacggtgc gcatcgtcac tgacagcagc agctgcctac ctccggggtg
     4561 ggcggaagag aacggcgtga gcgtgatccc tcaggggatc gtggtggacg ggacagccta
     4621 ccgcgagaac gtggacctct ccggtgacga gttctatcgc ctcctcgagt ggggctccac
     4681 cgccacctcc agccagccgc gaccggacgc tttcgtcacc gcctaccgcg cggcgctcga
     4741 tgccggcgac gagatggtgt cggtgcacat cgggtcggac ctcagcggca cgcttgacgc
     4801 tgcctacagc cacgcggccg ggctgcggac gcccgattcg ccggcccgca tccacctcgt
     4861 ggactcctgc agcgccggcc tggggttggg cttcctggtg gtggaggcgg cgcgcctcgc
     4921 ggccgagggc gctgaggccc tgtccatcgt gacggccctg gaagccatgg tgccgaagat
     4981 ccgcgtctac ttcatgctcc acaccatgac ctacctcgtg cgcggcggcc gggtgggtcg
     5041 cgccgcgggc ctggcagcct cgctcctgaa gctgcggccc atactcacca tcaatgacgg
     5101 cgagatcgcg ccggcggcac gcacacgcac cccgcacgcc gcggcagcgc agctctgggc
     5161 gctcatagac gcctctgccc ggcgcggcgt gcgctacgcc gggttccact acgggctgaa
     5221 ccgcgacgag gtggacggct ggcgccagga gttcacccgc cgctatggcc tggaggcatg
     5281 gctcacgcag ctcggaccgg cggtgggggc ccactcgggg ccgcgcgtcc tgggcgtggt
     5341 gctcgtcgag gagtgatgcg gccgcgcccg aggcccgctg tgtgcgccgg ccgctgtgcg
     5401 cgccacctac cgcgcgccac ctaccgcgcg ctcccgctgc tgcgggtcga ggccagtgcg
     5461 ggtcgaggtc gctgcgcgcc gtcgccgccc cggagtcact gtggtaccat aatggcgtca
     5521 tatcgactcc acacacgtgc ggaggccatc gtgcctgtaa gtctgtccat aaagaacgtc
     5581 ccggaggatg tggccgccca actccgcgac cgtgcgaaag ctcacagacg atcactccag
     5641 ggggagcttc tggcgatcgt cgaacaggcg gtcgccgcgc ccgcggaact gactcctgcg
     5701 ggactgaagg cctacgtcga ctccctaggt ctactgacgg acgatgactc ggtaggcatc
     5761 atcaggacag accgtgacgg ccgctgacga ggggatcgcg cccagcgggg acagcgcccc
     5821 gggcacatac ccacccgcgg gttcgcagcc gcttgtggta gtcgacgctt cggtgttcgg
     5881 agcccttgtg ttcagcgagt cgcgcagccg ggaagcagcg gatctgttgg ccgaagccac
     5941 tctgatctcc cccggtcttg tcaggtacga gatggccaac atcgggcgca cgaagatcat
     6001 gcgaaacccc gagagcggcg ccctcatcac ccgtgcgttc cgtcgctggt tggaactgcc
     6061 cgtgcgcttg gtgaccccag atttcggagg agtcttgagt ctcgcagtgg ccgagcagtt
     6121 gagcgcctac gacgccgcct atgtgtacgt agccgccgcg ctgggcgctc ccctcctgac
     6181 cttcgacgaa cagctcggcg cgacctggcg acgtatcagc ggctgaccgg ctgcgcgtct
     6241 cggcggctgc gcgtcttcgg ctcgtacaga tgcgctcggg tcagcggcag gtcggaccgg
     6301 agcccgttgg tgaacgtgac ctgatacacg cgggcgtcac cgtatttgaa gccggccgcc
     6361 gaggcgtgaa ggtagaagcg ccacatccgc acgaacgact cggagaagcg ccgccgcacc
     6421 tcgtcgacac agctttcgaa ccgttcggcc caggcgtcga gggtgcgggc gtagtggaga
     6481 cgcagattct ccacgtcggt ggccaccagg tccgtctgcc ccatggactc gcagatttgg
     6541 cccaaggtgg gtaggtagcc gccggggaag atgtacttgc cgatccacgg gtcaacgtcc
     6601 gcttcgcatt gcgttccgat cgtgtgtagg actcccaccg ctccaggggc caacaactct
     6661 cctactttcg agaagaactc cccgtagtac tccttaccca catgctcgaa catgccgatg
     6721 gacacgaact tgtcgaactc gccctcggcc tcccgatagt cgaggagttc gacgcgtacg
     6781 cggggataga gcccttcggc ccgcagacgt tcgttcgcga accggtgctg ctcgctggag
     6841 agtgtgtagc ccacgccttc cactccgtac tcccgggcgg cgtacagcag catccctccc
     6901 cagccacatc ccacgtccac caaccgctct cctt
//
LOCUS       DASRMJ010000249         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_527556, whole genome shotgun sequence.
ACCESSION   DASRMJ010000249 DASRMJ010000000
VERSION     DASRMJ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527556"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFD74_06895"
     CDS             complement(<1..655)
                     /locus_tag="VFD74_06895"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HZK49309.1"
                     /translation="MPRAGNGTGRLGDILIKSGALTKGKLDRALSYQKEHGSKLGEAL
                     IKLGYISDEQFAEALAAQKRLSVVRLEEVFPNPRVVTLLPEKFIRAREVIPVDFDRDA
                     LILAMVNPLDVVTLDDVRMMTGHDIVPVVATSSDFKDTLEYVFSGKGALDAREKDEEL
                     SFSHLAEERRDAEDVSVVQLVDGILESALKRRASDVHFEPQLYGMPVRIRVDGVLHQI
                     "
     gene            765..1481
                     /locus_tag="VFD74_06900"
     CDS             765..1481
                     /locus_tag="VFD74_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HZK49310.1"
                     /translation="MTLRCGPPTSKESAVLPIWDWTLILLLPAFALSLYAQIKVKSTF
                     ARYSKVPSGRGLTGARRLLDAGGLSHVPVELTQGQLSDHYDPRGKVLRLSADVANRDS
                     LAALGVAAHEVGHALQDADSYAPMRARSLLVRPASLGSNLGIILFIVGLFIGRNPMLM
                     NLGILLFSAAVLFTLVTLPVEFNASRRALALLTSQGILVSSEVDGARKVLSAAGLTYV
                     AAALMAIAQLARLLLISRSR"
     gene            complement(1593..1952)
                     /locus_tag="VFD74_06905"
     CDS             complement(1593..1952)
                     /locus_tag="VFD74_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK49311.1"
                     /translation="MARIVVADDDPDILNIVAMSLEAMGHEVHRATNGLEAVALTKEN
                     APNLVVMDLMMPEMNGYEATEALKADALTASVPIVALTAKAMRGDEERGRQAGVDAYL
                     TKPFRISQVIELVEQLL"
     gene            complement(1964..>2305)
                     /locus_tag="VFD74_06910"
     CDS             complement(1964..>2305)
                     /locus_tag="VFD74_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK49312.1"
                     /translation="SNAIKYTPETGRISVEARPTPDGLVEIRVSDTGVGIAAEDLKRV
                     FERFSQIDNSSTRSQGGTGLGLAITRDLIELHQGSIAVQSQPGKGSQFMFTIPQAGAG
                     REPRSKAPRLG"
BASE COUNT          361 a          771 c          712 g          461 t
ORIGIN      
        1 ttatctggtg aagcacgccg tccaccctga tgcgcacggg catgccgtag agttggggtt
       61 cgaagtgcac atcggaggcc cggcgtttga gggccgattc cagtatgcca tccaccaatt
      121 gcaccaccga gacgtcttcg gcgtcgcgcc gctcctcggc taggtggctg aacgagagct
      181 cctcatcttt ctccctggca tccagggcgc ccttgcccga gaacacgtac tcgagcgtgt
      241 ctttgaagtc gctcgaggtg gctacgaccg ggactatgtc gtgaccggtc atcatgcgca
      301 cgtcgtcgag ggtcacgacg tccagaggat tcaccatggc aagtatcagc gcgtctcgat
      361 cgaagtccac gggaatgacc tctcgggccc ggatgaactt ctcggggagc aatgtgacta
      421 cccgcggatt gggaaagacc tcctccaacc ggacgactga cagtctcttc tgggctgcca
      481 gcgcttcggc gaactgctcg tcgctgatgt agccgagctt gatgagggct tcgcccagct
      541 tcgacccatg ttctttctgg tagctcaagg cgcggtcgag cttgcccttg gtaagggccc
      601 cactcttgat gaggatgtcg cccagccgac cggtgccgtt gcccgctcga ggcatcgacg
      661 tcacctgctt cctatagggg gtgggggtac acgcgtcagg tgcatcatct ctgctatcat
      721 cataccgtcc gacggctaag gtatcgacag ttccgctccc ggacatgacc ctccggtgcg
      781 gtcccccgac aagcaaggag tccgccgtgc ttcccatctg ggattggaca ctcatactgc
      841 tactgcccgc cttcgctctg tctctctacg cgcagatcaa ggtgaagtcg acgttcgccc
      901 gctacagcaa ggtgccttcc gggcgcggcc tcaccggcgc gcgccggctg ctggatgcgg
      961 gcggtctctc tcacgtgccc gtcgaactca cgcagggcca actctccgac cactacgacc
     1021 ctcgaggcaa ggtgctccgc ctgagcgccg acgtggccaa tcgggactcg ctggccgccc
     1081 tgggggtcgc cgcgcacgag gtggggcacg ccttacagga cgccgacagc tacgcaccta
     1141 tgcgcgcccg ctcgctcctc gtacgccccg cgtcgttggg gagcaacttg ggcatcatcc
     1201 tattcatcgt gggtctgttc atcggacgca acccgatgct catgaacctc ggcatcctac
     1261 tcttctccgc ggccgtgttg ttcactctgg tcacgctccc ggtggagttc aatgcgtccc
     1321 gccgggcgct ggctctgctc acctcccagg gcatcctggt ctcgagcgag gtggacgggg
     1381 cgcgcaaggt gttgagtgcg gccggtctca cctacgttgc cgcggcgctc atggccatcg
     1441 cccagctggc gcgactgctt ctcatcagcc gtagtcgctg atccaaagac gggaacggcc
     1501 gcgcaaagcg acctgacaag ggccggcagg caggcgcggg aggcgggccg cgcgaggtcg
     1561 cccggcccag gccgccagcc tcaggccgcc cgttacagga gctgctccac caactcgatg
     1621 acctgcgaga tacggaaggg cttggtgagg tacgcgtcga cgccggcctg ccggccccgc
     1681 tcctcgtcgc cgcgcatggc cttagccgtg agcgccacga tcggcaccga ggccgtcagg
     1741 gcgtcggcct tcaacgcctc cgtagcctcg tagccgttca tctcgggcat catcagatcc
     1801 atgaccacca agttgggcgc gttctccttg gtcagcgcga ccgcctccag gccattcgtg
     1861 gccctatgga cttcgtgtcc catggcctcc agcgacatgg cgacgatgtt gaggatgtcc
     1921 gggtcgtcgt ccgcaacgac aatgcgagcc accgtgggcc tccctatcct agtctcggag
     1981 ccttgctgcg aggttctctg cctgcgccgg cctgtgggat cgtgaacatg aactgcgatc
     2041 ccttccccgg ttgggattgt accgctatcg agccctggtg gagttcgatc aggtcccgcg
     2101 tgatggcgag gccgagaccg gtgccgccct ggctgcgcgt ggatgagttg tctatctgac
     2161 tgaaacgttc gaagacgcgc ttgagatcct ctgccgctat gcccaccccg gtgtcgctca
     2221 cccggatctc caccaggccg tcgggggtgg gtcttgcttc gacgcttatg cggccggtct
     2281 cgggagtgta cttgatggcg ttact
//
LOCUS       DASRMJ010000250         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_139417, whole genome shotgun sequence.
ACCESSION   DASRMJ010000250 DASRMJ010000000
VERSION     DASRMJ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139417"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1439)
                     /locus_tag="VFD74_06915"
     CDS             complement(90..1439)
                     /locus_tag="VFD74_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZK49313.1"
                     /translation="MFPVNPKGGEIFGLPAYSSIDDVPEAPELVLIALPAKLVPDVVD
                     RCGKKGAKAVVVISGGFDEASEEGALMAQEAARIASEHGLAMVGPNSLGAMCSWNKFY
                     GTGAAILQPLVGPASFLSQSGSMGMQLMQACETHQAGLGKYVGVGNEALVDAADLIEY
                     FRTDPRTGVVLAYVEGLVDGRRFLETCRDTSAAKPVVLLRGGASEFGRKAAASHTGAM
                     AASQRVFEAVVRQSGLMYTNDPDEFLDLAYCLSYLPLPRGTRIAIVTFGGGWGVLSAD
                     EVYRNGLQLAELAPNVIDELNALLPPFWSHANPIDLVGMFTAKGEPEGAVHAVTPSDE
                     VDAVVVLGVLGIVSQPLKTMAVVERLHKEYGIDVSSIDPPDVDEYRQREEDFIRKVAE
                     HMEDYEKPIINVSFTPMEKSVFDVGRKYSTVVLPSPLRAVRVLAQMARYREYLDSLA"
     gene            complement(1375..>2203)
                     /locus_tag="VFD74_06920"
     CDS             complement(1375..>2203)
                     /locus_tag="VFD74_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZK49314.1"
                     /translation="LLSAYGISVTPGAVAATVEEALDIAGDVGFPVAMKGSSHQILHK
                     SDAGLVLGDIRGEVGLRNAWDLLQERGAGRLDGILVEKMIPAGREFVIGMSRDEQFGP
                     LVMFGVGGVLTEALKDVVFGVAPLDAREAARMLDAIRAKELLGPFRGAPAVDRDALAR
                     VITTIGRMALENPEIQEVDINPILLDGGTPVAVDALVTLGPPADALPPRLPVSLENVR
                     YIFDPKSVALVGASNDPTKWGGLILASIISGGYGGQDLSRQSQGWRDLRPSRLLQHR"
BASE COUNT          362 a          763 c          718 g          360 t
ORIGIN      
        1 caagtatggc agacgggcgc ggcgtgcgcc gcgcgccacc ccgcatccgt tctgcgaagg
       61 gcggagaatg tgacccgtgg ccgccggcct caggccagcg agtccaggta ttcgcggtac
      121 cgagccatct gcgcgagcac ccgcaccgcc cggagcggcg atggcaggac tacggtcgag
      181 tatttgcggc ccacatcgaa gacgctcttc tccatcggcg tgaacgagac gttgatgatg
      241 ggcttctcgt agtcctccat gtgttccgcg accttgcgga tgaagtcctc ctcccgctgc
      301 cggtactcgt ccacgtcggg cgggtcgatg ctgctcacgt ctatgccgta ctccttgtgc
      361 agccgctcga ctaccgccat cgtcttcagg ggctgactca cgattcccag gacacccagc
      421 acgaccactg cgtccacctc atccgagggc gttactgcgt gcaccgctcc ctccggctcg
      481 cccttggccg tgaacatgcc gaccaggtcg atggggttcg cgtgactcca gaagggcggc
      541 agcagcgcgt tgagctcgtc gatcacgttc ggcgccagct cggccagctg aaggccgttg
      601 cggtagacct cgtcggcgct gaggactccc cagcctccac cgaaggtgac gatagcgatc
      661 cgcgtgcccc ggggcagcgg caggtaagac aggcagtacg cgagatcgag gaactcgtcc
      721 gggtcgttgg tgtacatgag acccgactgc cgcaccaccg cctcgaatac cctctgcgag
      781 gcggccatgg ccccggtgtg cgacgccgcc gccttacggc cgaactcgct ggctccgccg
      841 cggagcagca ccaccggctt ggcggcgctg gtgtcgcggc aggtctccag gaagcgccgc
      901 ccgtcgacca ggccctcgac gtatgcaagg accacgcccg tccgcggatc tgtgcggaag
      961 tactcgatga ggtcggcggc gtccacgagg gcctcgttgc ccacgccgac gtatttcccc
     1021 aggccggcct ggtgcgtctc gcaggcctgc atgagctgca tgcccatgct gcccgactgg
     1081 gagaggaagc tcgccggccc cactagcggc tgcaggatcg cggcgccggt gccgtagaac
     1141 ttgttccagg agcacatggc gcccagggag ttcgggccca ccatggcgag cccatgctcg
     1201 gaggcgatcc gcgccgcctc ctgagccatg agggcacctt cttcactcgc ctcgtcgaag
     1261 ccgccggaga tcacgaccac cgccttggcg cccttcttgc cgcagcggtc caccacgtcc
     1321 gggacgagct tggcggggag cgcgatcagg acaagttcag gagcctccgg cacgtcatct
     1381 atgctggagt aggcgggaag gccgaagatc tctccaccct tgggattgac gggaaagatc
     1441 ttgccccccg tacccgccgg agatgatgct ggcgagaatc aggccgcccc acttggtcgg
     1501 gtcgttgctt gcgcccacca gggccacgct cttggggtcg aagatgtacc tcacgttttc
     1561 caggctgact ggaagacggg gcgggagagc gtccgccgga ggccctagcg taaccagagc
     1621 gtcaacggcc acgggagtgc cgccgtcgag caggatggga ttgatgtcga cttcctggat
     1681 ctccggattc tccagcgcca tgcgcccgat ggtggtgatc acccgcgcaa gagcgtcgcg
     1741 gtcaaccgcc ggggcgcctc ggaagggccc cagcagctcc ttggcccgga tggcgtcgag
     1801 catccgggcg gcctcgcgcg cgtccagggg cgcaacgccg aacaccacat ccttgagcgc
     1861 ctcggtcagc accccgccta cgccgaacat gaccaagggc ccgaactgct cgtcacggct
     1921 catgccgatg acgaactcgc gcccggcggg gatcatcttc tccacgagga tgccgtccag
     1981 gcggccggct cctcgctcct gaaggaggtc ccaggcgttt ctcaggccga cctcgccgcg
     2041 aatgtcgccc agcaccaggc cggcgtcact cttgtgcagg atctggtgcg aggagccctt
     2101 catggcgacg gggaaaccca cgtctccggc gatatcgaga gcttcctcta ccgtggcggc
     2161 cactgcgccg ggcgtcacgc taatgccata agcggagagg agg
//
LOCUS       DASRMJ010000251         6590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_165045, whole genome shotgun sequence.
ACCESSION   DASRMJ010000251 DASRMJ010000000
VERSION     DASRMJ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165045"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFD74_06925"
     CDS             complement(<1..725)
                     /locus_tag="VFD74_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZK49315.1"
                     /translation="MSYYVFKDTAACPLAVRHHRDEGESVVEITRRKQGRIYQQVVEH
                     IQTLVGEGVLSPGDQLLPERQLAEKLGVSRGALREALSVLISQGLIEVTPGGGSVIRE
                     AKIEDLTDPLAAVILKERESVRDLLEARVILEVGAVRLAAERGDASDFYRIKQAALET
                     NECMRGGQSSDEADIAFHTSIVQASHNPVLVSVMTMISALVSEVYGPSRRRLIEDPEK
                     LEYCAQRHLDIFEAVHSRDPEAAV"
     gene            896..1750
                     /locus_tag="VFD74_06930"
     CDS             896..1750
                     /locus_tag="VFD74_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZK49316.1"
                     /translation="MSDTMTTYTGTHDYIASAELMQTVNVAVALARPLLIKGEPGTGK
                     TMLAQSIAAGLGMPLLVWNVKSTSKGQDGLYVYDTVQRLYDSQFGDGDVADISRYIKL
                     GPLGRAFSSEERVVLLIDEIDKADLEFPNDLLWELDQMEFTVAETGESVRAGRRPVVI
                     ITSNAEKELPDAFLRRCIFHYIEFPSDELMRRIVKVHHPDLEARLLDQAMETFYRLRE
                     IPGIQKKPSTSELIDWLQALVIGGVEPEEISKRLPFLGVVLKKDKDVEAAAFAARHPG
                     ATRPLPRS"
     gene            1765..2973
                     /locus_tag="VFD74_06935"
     CDS             1765..2973
                     /locus_tag="VFD74_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZK49317.1"
                     /translation="MFVEFFFLLRARKVPVALTEWLTLTEALSQGLALSSLSAFYSLG
                     RSTLVKSEAYFDQYDQAFGEAFAGVDTPAEIHDEVWKWLEEGAHLPGLTEVERAALAA
                     GMAGLDLDELRRLFEERLREQKEAHHGGSRWIGTGGRSPFGHSGFHPGGLRVGGEGFG
                     RSAVKVAAERRYRGYRTDAAVGVRQFETALRRLRQLSSKNEGPLDELDLEGTINRTAD
                     HAGALSLVWRRPRRNQTRVLLLMDAGGSMHAHIGACNRLFTAVDRATHLKDLRRYYFH
                     NCVYDNLYLDSTCHPRNSIPTARVLQELSPDYKLVIVGDASMAPSELLEPNGIIWWSA
                     SNEEPGIEWLRRLNRHFHRVVWLNPIGERSWESIYGRDTIAIIRSIFPMFELTVDGFT
                     AAVRNLMVRR"
     gene            3272..3541
                     /locus_tag="VFD74_06940"
     CDS             3272..3541
                     /locus_tag="VFD74_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HZK49318.1"
                     /translation="MANEPQPKGDGVAYWKANLRLITILLVIWAAVSYGAAIFLANPF
                     YKIQFFQVNLAFWFAHQGSMIVFVVLIFVYAKMMDKIDRDYDVHE"
     gene            3551..5224
                     /locus_tag="VFD74_06945"
     CDS             3551..5224
                     /locus_tag="VFD74_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZK49319.1"
                     /translation="MSVEVLTYIIVGITFTAYIYIGYMNRVGTTSGFYVAGHGVPAIA
                     NGAATAADWMSAASFISMAGLISFLGYNGTFYLMGWTGGYVLLALLLAPYLRKFGKYT
                     VPDFVGDRYYSHTARVVAAVCAIFVSLTYVAGQMRGVGIVFSRFLGLPIGWGVVVGMV
                     IVAFFAVLGGMKGITWTQVAQYSVLICAYLIPAIAIAWIVTGNPIPQLALTFSDIVPK
                     LNQLQTDLGLIEYAKPFQGKGMLDVFMITVALMIGTAGLPHIIVRFYTVPSVRAARYS
                     AGWALLFIALLYTTAPAIAVFAKYNLINELNGKTPAQVQELDWAVKWETTKLLKFEDK
                     NADGLLTMTAAADTNEIEIDKDIIVLSTPEVARLSPIVIALVAAGGLAAALSTAAGLL
                     LVISSSLAHDIYYRLINPNATEARRLLIGRLMIFVAVVFAGYFGIKPPGFVAQVVAFA
                     FGLAAASFFPVIILGIFDKRMNKEGAIAGMVIGMGFTMAYIIGNRFMGMDPWFFNVSA
                     EGIGTFGALLNFTACMVVSRLTPPPPPEIQDLVESVRIPRGSSQAIEHY"
     gene            5468..5842
                     /locus_tag="VFD74_06950"
                     /pseudo
     CDS             5468..5842
                     /locus_tag="VFD74_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            5847..>6590
                     /locus_tag="VFD74_06955"
     CDS             5847..>6590
                     /locus_tag="VFD74_06955"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49320.1"
                     /translation="MGRPLTNSASRSTQRTPHIGRTSAACQTGAASRRCKQTASPKSC
                     HLSSNVFAPVFPGAVGRTPGLWSTYLADPEHEREGASALFHVVHRGDDGSADGYVSYR
                     VKSDWVGGIPDSTLIVHDVLAPGPEVYAALWRYCLDVDLVATVSTVWAPVDEPLRWLL
                     ADPRRLRVNRLNDGLWLRILDVPAALAAREYGGGHGGGRGESGDGAQGRAGGDALVLD
                     IADAFRPATAGRYLLEAGPDGAACCRTMRP"
BASE COUNT         1134 a         2231 c         2047 g         1178 t
ORIGIN      
        1 acggccgcct ccgggtcacg gctgtgcaca gcctcgaaga tgtcgagatg ccgctgcgca
       61 cagtattcga gcttctcggg atcctcgatc agccttctgc ggctgggccc gtacacctcg
      121 ctcacgagag cgctgatcat ggtcatgaca ctgacgagca ccggattgtg cgacgcctgg
      181 acgatactcg tgtgaaacgc gatatcggct tcgtccgacg attgtccgcc cctcatgcac
      241 tcgttcgtct cgagcgctgc ctgcttgatc cggtagaagt cgctcgcatc gccgcgttcg
      301 gcggccaacc ggaccgcacc gacttccagt atgacacggg cctcgagcag gtcccgcacg
      361 ctttcgcgct ccttgaggat gacggcggcc agggggtccg tgaggtcttc tatcttagcc
      421 tcgcggatca ccgagccccc gccgggagtg acctcgatca ggccctgcga gatcagcacc
      481 gagagcgcct cgcggagggc tccgcggctc acccccagct tctctgccag ctggcgttcg
      541 gggagcagtt ggtctccagg agacagtacg ccttcgccta ccaacgtctg gatgtgctcc
      601 acgacttgtt ggtagatgcg cccctgcttc ctgcgggtaa tctccactac tgactcgccc
      661 tcgtcgcgat ggtgccgaac ggccaaggga cacgcggcgg tgtccttgaa gacatagtaa
      721 ctcattcggt ggcactggtc ctaccggtgg tctaccatca tccttcccct ggaggcgccg
      781 atacgcctgt gggacaatgt agtctgaaca atccccgatc gacggcgccg catccgccgg
      841 catcgacatg ccggcttcga cgcacgtcgc cacccgatcc gccgtggagg agaccttgag
      901 cgacacaatg accacctaca ccggcacgca cgactacatc gcctccgccg agttgatgca
      961 gaccgtcaac gtcgccgtcg ccctggcccg cccgctgctg atcaaggggg aacccggcac
     1021 cggcaagacg atgctggcac agagcatcgc cgcgggcctc ggcatgcccc ttctcgtgtg
     1081 gaacgtgaag tccacgagca aaggccaaga cggcctctac gtctacgaca cggtgcagcg
     1141 cctctacgac agtcagttcg gcgacgggga cgtggccgat atctccaggt acataaagtt
     1201 ggggcctctc gggagggctt tctcgtcgga ggagcgcgtc gtgcttctca tcgacgagat
     1261 cgacaaggcg gatctggagt tccccaacga cctcctgtgg gaactcgacc agatggagtt
     1321 caccgtagcc gagaccggcg agagcgtgcg tgcgggccgc aggcccgtcg tgatcatcac
     1381 ttcgaacgcc gagaaggagc ttcccgacgc cttcctccgg cgctgcatct tccactacat
     1441 cgagtttccg agcgacgaac tcatgcgccg catcgtgaag gtgcaccatc ccgacctcga
     1501 agcccgactg ctcgaccagg ccatggagac gttctatcgg ctgcgcgaga tccccggcat
     1561 ccagaagaag ccgtcgacga gcgagctcat cgactggcta caggccctgg tcatcggtgg
     1621 cgtcgagccc gaggagatct ccaagcgtct gcccttcctc ggggtggtgc tgaagaagga
     1681 caaggacgtg gaggccgcag ccttcgccgc gcgacaccct ggagcgaccc gacccctccc
     1741 gcgttcctag aagcgagcgc aggcgtgttc gtcgagttct tcttcctgct tcgagcccga
     1801 aaggtaccgg tggccctcac cgagtggctg acgctgaccg aggctctgtc gcagggactg
     1861 gcgctctcca gcctgagcgc cttctactct ctcggccgct ccactctggt caagtcggaa
     1921 gcgtacttcg accaatacga ccaggctttc ggggaggcct tcgccggtgt cgacacgccc
     1981 gccgagatac acgacgaggt gtggaagtgg ttggaggaag gagcgcacct gcccggattg
     2041 accgaggtgg agcgggcggc gctcgcggcg ggtatggcag gcctcgacct cgacgagttg
     2101 cgccgcctct tcgaggagcg tctccgtgaa cagaaggagg cccaccacgg aggttctcgc
     2161 tggatcggta ccggcggccg ctcgcccttc ggtcactccg gattccaccc gggcggtctg
     2221 cgcgtcggag gggaaggctt cgggcgatcc gcggtcaagg tggcggccga acggcgctat
     2281 cgcggctacc gcacggacgc cgcggtgggc gtacgccagt tcgagacagc cctgcgccgc
     2341 ctccgtcagc tctcgagcaa gaacgaggga cctctcgacg agctggatct cgagggcacc
     2401 atcaacagga cggccgatca cgcgggagcg ctctctctgg tgtggcggcg gccgcgcagg
     2461 aaccagaccc gggtgctcct gctcatggac gccggcggct ctatgcatgc ccatatcggc
     2521 gcctgcaacc ggctgttcac ggcggtcgac cgggcgaccc acctgaaaga cctgcgccgc
     2581 tactacttcc acaactgcgt ctacgacaac ctctacctgg actccacctg ccatccccgc
     2641 aactcgatac ccaccgcccg cgtgctccag gaactctccc ccgactacaa gctggtgatc
     2701 gtgggggacg cgtccatggc tccgtcggaa ctgctggagc cgaatggcat catctggtgg
     2761 agtgcgagca acgaggagcc cgggatcgag tggcttcgcc gcctcaaccg ccatttccac
     2821 cgcgtagtgt ggctgaaccc gataggcgaa cgatcgtggg agagcatcta cggtcgcgac
     2881 accatcgcca tcatccggag catcttccct atgttcgaac tcaccgtgga cgggttcacg
     2941 gcggcggtcc gcaacctgat ggtgcgacgc tagaacctct cgtcgccgtc aaccgaccgc
     3001 tcggcgggcg ccgcttctgc cgcccgccga accaccgcga ccactcatct tccgcacggg
     3061 tccgctcgaa ggatacccat gcttctcgcc ttccgtgccc gcggcccctc ttctagactc
     3121 aagcaggact acaggccggg tttcgcgcat cccggaccca gcccaaggag gtgccgaggg
     3181 cacggaacta ccgaacagga gggggttgcg cgggagtacg ggagtcgtct caactacttc
     3241 aaacgagcga ctgattcaaa ggggggatga aatggcaaac gaaccccagc caaagggtga
     3301 cggggtagct tactggaagg ccaatctcag gctgatcacc atcttgctcg tcatatgggc
     3361 agcggtctcc tacggtgcag cgattttcct ggccaacccg ttctacaaga tccagttctt
     3421 ccaggtaaac cttgctttct ggttcgcaca tcaggggtcg atgatcgtgt tcgtcgtctt
     3481 gattttcgta tacgccaaga tgatggacaa gatcgaccgc gactacgacg tccatgagtg
     3541 aggtgaagta atgtcggtag aagttctaac ttacataatc gtcggcatca cgttcaccgc
     3601 ctacatctac atcggctaca tgaaccgagt aggcaccacc agcggtttct acgtggcggg
     3661 ccatggcgtc cccgcgatcg cgaacggcgc ggccaccgcg gctgactgga tgtcggcggc
     3721 gtcgttcatc tccatggcgg gtctcatctc cttcctcggg tacaacggta ccttctacct
     3781 catggggtgg acgggcggct acgtgctcct ggccctgctc ctcgctccct acctgcgcaa
     3841 gttcggcaag tacacggtgc cggacttcgt cggtgaccgc tactactcgc acaccgcgcg
     3901 cgtggtggcg gccgtctgcg ccatcttcgt ctcgctcact tatgtggccg ggcagatgcg
     3961 cggcgtgggt atcgtgttca gccgcttcct cggcctgccc atcggttggg gcgtcgtggt
     4021 ggggatggtc atcgtggcct tctttgccgt gctcggcggg atgaagggca tcacctggac
     4081 ccaggtggcg cagtactcgg tgctgatctg tgcctacctg atcccggcca tcgccatcgc
     4141 gtggatcgtg accggcaacc ccattccgca gttggccctc accttcagcg acatcgtgcc
     4201 gaagctgaac cagctgcaga ccgacctcgg actcattgag tacgcgaagc ccttccaagg
     4261 caagggcatg ctcgacgtgt tcatgatcac tgtggccctg atgatcggga ccgccggtct
     4321 gccgcacatc attgtccgct tctacacggt gccgtcggtg cgcgccgccc gctactcggc
     4381 cggttgggcg ctcctgttca tcgccctgct ctacaccacg gccccggcga tcgccgtgtt
     4441 cgccaagtac aacctgatca acgagctgaa cggcaagaca cccgcgcagg tgcaggaact
     4501 cgactgggcc gtcaagtggg agacgaccaa actgctcaag ttcgaagaca agaacgccga
     4561 cggcctgctg acaatgaccg cagcggccga caccaatgag atcgaaatcg acaaggacat
     4621 catcgtcctg tcgacgccgg aagtcgccag gctctcgccg atcgtaatcg cgctggtggc
     4681 ggccggtggt cttgccgccg ccctttcgac cgcggccggc ctgctgctcg tgatctcgag
     4741 ctccctggcc cacgacatct actaccgcct gatcaacccg aacgccaccg aggcgcgtcg
     4801 gctcttgatc ggccgcctca tgatcttcgt ggccgttgtg ttcgccggct acttcggcat
     4861 caaaccgccc ggctttgtgg ctcaggtggt ggccttcgcc ttcgggttgg ccgctgcgag
     4921 cttcttcccg gtcatcatcc ttgggatctt tgacaagcgg atgaacaagg aaggagccat
     4981 cgccgggatg gtcatcggga tgggcttcac gatggcctac atcatcggca accgcttcat
     5041 gggtatggat ccgtggttct tcaacgtgag tgcggaaggc atcgggacct tcggcgcgtt
     5101 gctgaacttc acagcttgca tggtggtctc tcgcctgacg ccgccgccgc cgcccgagat
     5161 ccaagatctc gtggagtcgg tgcggatccc gcgtggctca agccaggcca tcgagcacta
     5221 ctagaagaga acgccgcggc gctcgcaccg agcgtcgcat ggaaggaccc gaaaggggcg
     5281 cggaaacgcg cccctttcgc ttctgcccct cacgcaagtg acaccgacga acgtggacct
     5341 acgccgtccg ggggggcgtg ctactctggc ccgcggcgct tgccgcgggc gcacccgcgt
     5401 tgcgccacac gcgcgacgca tcagactacc gggacacgcg atccaatcca cgccggaggc
     5461 gcctcccatg ccgatcgagt accgccccat cttggaggac gaattcgtcc atttcgccac
     5521 ggccgccgtg ggagctttcg ggaccgccat gtccggccaa gacctccggg ccgagaggtc
     5581 cgctatcgag ttcgaccgca cgctcgccgc cttcgagaag ggccggatcg ttgggacgac
     5641 ggccactctg accctcgatg tgacgctgcc gggagggagg gcgctgcccg ccggggggct
     5701 cacgtggaca ggagtgctcc ccacgcaccg ccggcaaggc gtgctcaaga ccatggtcga
     5761 ggcacaactc caggggtccg ccctgcggga ggagccgctc agcatccttc aagcctcgga
     5821 gtccaccatt tacggccggt tcgggtatgg ggcggccact tacgaactcg gcttctcgat
     5881 cgacacagcg cactccgcat atcggcagga cgtcggcggc gtgtcagacc ggggcagctt
     5941 cgaggcgctg caagcagact gcgtcgccga agtcctgcca cctctcttcc aacgtcttcg
     6001 cccccgtctt cccgggcgcc gtcggccgca cgccgggcct ctggagcacg tacctcgccg
     6061 accccgagca cgagcgtgag ggagcgagtg cactgttcca cgtggtccac cggggcgacg
     6121 atggatcagc cgacggctac gtgagctacc gcgtcaagtc ggactgggtc ggcggcatac
     6181 ccgacagcac gctaatcgtg cacgacgtgc tggccccggg cccagaggtc tacgccgccc
     6241 tctggcgcta ctgtctcgac gtggatctcg tcgccaccgt gtccaccgtc tgggcccccg
     6301 tcgacgaacc cctgcgatgg ctgctcgccg atccccgaag gctgcgggtg aaccgattga
     6361 acgacggcct gtggctgcgg atcctcgacg tgccggcggc gctggccgcc cgcgaatacg
     6421 gcgggggcca cggcggaggc aggggtgaaa gtggcgacgg cgcccaaggc cgcgcgggcg
     6481 gggacgccct ggtgctcgac atcgccgacg cgttccgccc cgccacagcc gggcgctacc
     6541 tgctcgaggc cgggccggac ggcgccgcgt gctgccgcac catgcgcccg
//
LOCUS       DASRMJ010000252         4112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_272111, whole genome shotgun sequence.
ACCESSION   DASRMJ010000252 DASRMJ010000000
VERSION     DASRMJ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272111"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFD74_06960"
     CDS             <1..341
                     /locus_tag="VFD74_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZK49321.1"
                     /translation="CDPDEVDFIIPGNDDAIRSCGLIISTLARAVEEGRQKVTAREFT
                     AAVAAPVVVETPQAEAPVAEAPVAEATAEELPVEELPVEELPAEEAAADVAVEESDET
                     TAAEAVEETE"
     gene            461..1063
                     /gene="tsf"
                     /locus_tag="VFD74_06965"
     CDS             461..1063
                     /gene="tsf"
                     /locus_tag="VFD74_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZK49322.1"
                     /translation="MSISAALVKDLREKCGAGMMDCKRALEETNGDLDEAVKLLRMKG
                     MAAADKKSARAAEEGLVESYIHGGGKIGVLVEVNCETDFVARNEEFRTFVHDVAMHIA
                     ASGPSYVSREQIPEAMVAAELEIYTAQARETGKPETVLAKIAEGKLNKWYEQVTLLEQ
                     PFVKDPDKTIDVLRRELVAKVGENIEVRRFARFRLGEDLS"
     gene            1145..1930
                     /gene="pyrH"
                     /locus_tag="VFD74_06970"
     CDS             1145..1930
                     /gene="pyrH"
                     /locus_tag="VFD74_06970"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZK49323.1"
                     /translation="MSENEAGAGKAVFKRVLLKLSGEALVGDAVYGIDPERMIAVAGE
                     IRSCVELGVETAVVVGAGNIFRGVAGSAKGMDRTAADYMGMIATVINAMALQDALEKM
                     DVMTRVQSAIEMQEVAEPYIRRRAVRHLEKGRVVIFAAGTGNPYFTTDTAAALRALEI
                     GAEAILMGKKKFDGVYDADPELHPEAELIPLLTHREALERGLKVMDSTALSLCMDNNL
                     PIFVFNMNTPSNISRVLRGERVGTVVSCDVGAAAGAASWGSRP"
     gene            1970..2527
                     /gene="frr"
                     /locus_tag="VFD74_06975"
     CDS             1970..2527
                     /gene="frr"
                     /locus_tag="VFD74_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006992108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZK49324.1"
                     /translation="MIDELRDDARRRMDGAIEALRTEFAGVRTGRASTALLDRIYVEY
                     YGTRTPLKQLANVSTPDPRLISITVFDKSAVNSVVRAIQESDLGLNPNVDGTIVRLNI
                     PALNEERRRDLVKLVRSMSEDGRVAVRNVRRDVIQDLKELKKDGEISEDDERRAEDEV
                     QKLTDEHVERINELLQRKEEEILEV"
     gene            2527..3315
                     /gene="uppS"
                     /locus_tag="VFD74_06980"
     CDS             2527..3315
                     /gene="uppS"
                     /locus_tag="VFD74_06980"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZK49325.1"
                     /translation="MGIFARLKGLGGRTRPRGPFFQGEAPSPERVPHHVGIIMDGNGR
                     WASRRGLPAMAGHREGAQALKRTVEAAIEAGVHQLTVYSFSTENWGRPADEVSGLMDL
                     MGEMLEREVPRLHEQGVRIAFIGSRDGVDPKLVRRFEDAEATTAANRTLRLFVAFNYG
                     GRREIVDAVKLAMREGLTSEQLDEDAVSARMYAPEMGEPDLIIRTSGELRLSNFLLWE
                     SAYSELYFSPLLWPDFDRTEFARAIEDYAGRERRFGGRRTEDLA"
     gene            3308..>4112
                     /locus_tag="VFD74_06985"
     CDS             3308..>4112
                     /locus_tag="VFD74_06985"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZK49326.1"
                     /translation="MLRARLLVALVGIPLGVALIVVGGAAYAVALGLLMLLGLHEYYT
                     LVRPYRPNLLVGYVAAIGVLVAAYFAGVPGMAGAMAASLVLFFLWGMGGRLGDHLVGR
                     MAVTAFGLAWVVMGFGHLLLLRGLENGMTLTILAVGATWTNDTLAYFVGRFFGRRKMA
                     RRISPKKTVEGAIGGLVGAVLFALALKAYTPWLPVREAAIMGAVIGVTGQWGDLFESM
                     VKRDLRRKDSGRLLPGHGGVLDRFDSLLFVGAATYWLSVLLLGDLVQGGG"
BASE COUNT          733 a         1205 c         1466 g          708 t
ORIGIN      
        1 attgcgatcc cgacgaggtg gacttcatca tccccgggaa cgacgacgcc atccgctcgt
       61 gcgggctcat catcagcact ctcgcacgcg ctgtggaaga ggggcgccag aaggtgacgg
      121 ctcgtgagtt cacggccgcg gtcgccgcgc cggttgtcgt ggagaccccg caagccgaag
      181 cccctgtggc cgaagccccg gtagccgaag ccacggccga agagcttccc gtcgaagaac
      241 ttcccgtcga ggagcttccc gccgaggagg ccgcagccga tgtcgctgtc gaggagtcgg
      301 acgagaccac cgcagcggaa gccgtggagg agaccgagta gccgacggtg aacggccgcg
      361 gaacggggcg cgagcgcccg atgaatcgga ctgccgtcgc gtctgaacgt catggaaaca
      421 cgaggagacg gcccgactgg gccgttgtgg aggatagggt atgagcatta gtgccgcatt
      481 ggtgaaagac ctgcgcgaga agtgcggtgc cggcatgatg gactgcaagc gggcgcttga
      541 ggagaccaat ggcgacctcg acgaggcagt caagcttctc aggatgaagg gcatggccgc
      601 cgccgacaag aagtcggcgc gggcggcgga ggaaggtctc gtcgagtcct acatccacgg
      661 gggcggcaag atcggcgtgt tggtggaggt caactgcgag accgacttcg tggcacgcaa
      721 tgaggagttc cgcaccttcg tgcacgacgt ggcgatgcat atcgccgcga gcggcccctc
      781 ctatgtgagc cgcgagcaga tccccgaggc tatggtggcc gccgagctcg agatctacac
      841 ggcccaggca cgcgagacgg gcaagccgga gacggtcttg gctaagatcg ccgaaggcaa
      901 gctgaacaag tggtatgagc aggtgaccct actcgagcag ccgttcgtga aggatcccga
      961 caagaccatc gacgtccttc gcagggagct cgtcgcgaag gtcggcgaga acatagaggt
     1021 gcgtcgcttc gcgcggttcc ggctgggcga ggatctcagc tgaggagtcg gggcggcgcc
     1081 tgaggcgccg cccctttttt ttgtatcatg ggcgagtcag cggtgatcgt aagaggggtg
     1141 aggagtgtcc gagaatgagg cgggagccgg caaggccgtt ttcaaacggg tcctactcaa
     1201 actgagtggc gaagcgctgg taggggatgc tgtgtacggg atcgacccgg agcggatgat
     1261 cgccgtggcc ggagagatcc gtagctgtgt ggagctgggg gtagagaccg ccgtggtcgt
     1321 gggggcgggc aacatcttcc gaggcgtggc aggaagcgcg aagggcatgg atcgcacggc
     1381 cgccgactac atggggatga tcgccacggt catcaacgcc atggctcttc aggatgccct
     1441 cgagaagatg gatgtgatga ccagggtcca gtccgccatc gagatgcaag aggtcgcgga
     1501 accgtatatc aggcggcggg cggtgcgtca tcttgagaag ggccgtgtgg tgatcttcgc
     1561 ggcgggcacc ggcaatccct acttcaccac tgacacggcc gccgcactcc gggcgctgga
     1621 gatcggtgct gaagccatcc tgatggggaa gaagaagttc gatggcgtct acgacgccga
     1681 cccggagcta cacccggaag ccgagctgat cccgttgctc actcacaggg aggctctgga
     1741 gcgcggactc aaggtgatgg actctacggc cctgtcgttg tgcatggaca acaacctgcc
     1801 gatcttcgtg ttcaacatga ataccccgtc caatatcagc cgcgtgcttc gcggtgagcg
     1861 cgttggcacg gtggtatcat gtgacgtcgg tgcggctgcg ggtgccgcct cctgggggag
     1921 cagaccctag accaccggcg ggcactgcgg tcggggagga gcgaggagca tgatcgacga
     1981 attgcgggat gacgcgaggc gtcgcatgga tggggcgatc gaggccctgc gtacggagtt
     2041 cgccggcgtt cgcaccggca gggcttctac ggcgctgttg gatcgcatct atgtcgagta
     2101 ctacggaacg cgcaccccgc tcaagcagct cgccaacgtc tccacgcctg accctcggct
     2161 catctccatc accgtcttcg acaagtccgc ggtgaactcg gtggttcgag ccatccagga
     2221 gtccgatctg ggcttgaatc cgaacgtgga cggcacgatc gtgcgcttga acatccccgc
     2281 gctcaacgaa gagcgcagga gagacctcgt gaagctggtg cggagcatgt cggaggacgg
     2341 ccgggtagct gtgcggaacg tgcgccgcga cgtcatccaa gacctcaagg aactgaagaa
     2401 ggacggtgag atctccgagg acgacgagcg cagagcggag gatgaggtgc agaagctcac
     2461 cgatgagcac gtagagcgca tcaacgagct ccttcagcgc aaggaggagg agatcctcga
     2521 ggtctgatgg gtatctttgc tcgactcaag ggtcttggcg ggcgcacgcg cccgcggggc
     2581 cctttttttc aaggggaggc gccttcgccc gagagggtgc cgcaccacgt gggcatcatc
     2641 atggacggca acggtcgctg ggcgtctcgg cgggggctgc ctgcgatggc ggggcacagg
     2701 gaaggcgcgc aggcgctgaa gcgcacggta gaagcagcga tcgaggcggg cgtgcaccag
     2761 ctcaccgtgt actccttctc taccgagaac tggggccgcc ccgcggacga ggtgagcgga
     2821 ctcatggacc tcatgggcga gatgctcgaa cgtgaggtgc cccgactcca tgaacaaggc
     2881 gtacgtatcg ccttcatagg ttcgcgcgat ggagtcgacc cgaaactcgt acggcggttc
     2941 gaagatgcgg aggccaccac cgccgccaac cggactctcc gactgttcgt ggccttcaac
     3001 tacggcgggc gcagggagat cgtcgatgct gtcaaactgg cgatgcgtga agggctgacg
     3061 agcgaacagc tcgacgagga tgctgtgtcg gcgcgcatgt acgcgccgga gatgggcgag
     3121 cccgatctga tcatccgcac ttcgggcgag ttgcgcctga gcaacttcct gctctgggag
     3181 tcggcgtact ctgaactcta cttcagtccg cttctctggc ccgacttcga ccggacggag
     3241 ttcgcccgcg caatcgaaga ctacgccggg cgcgagcggc gcttcggcgg gcgccggacg
     3301 gaggatcttg cttagggccc gcctgctcgt agcgctggtc ggcatccccc ttggcgtcgc
     3361 tctcatcgtc gtgggaggcg ccgcctacgc ggtggccctg gggctgctca tgcttctggg
     3421 gcttcacgag tactacaccc tcgtgaggcc ctaccgcccc aatcttctcg tgggctacgt
     3481 ggcggccatc ggagtgctgg tcgcggccta cttcgctggc gtccctggta tggcgggcgc
     3541 catggcggcc tccctcgtcc tgttcttcct gtggggcatg ggaggccggc tcggcgatca
     3601 cctggtgggt cgcatggcgg tgacggcttt cggtctcgcg tgggtggtga tggggttcgg
     3661 ccacctgctc ctgctccgcg gtctcgagaa cgggatgaca ctgacgatcc tcgccgtcgg
     3721 ggcgacctgg accaacgaca ctctggcgta cttcgtggga cggttcttcg ggcgccgcaa
     3781 gatggcgcgt cgcatctcgc cgaagaagac cgtcgaaggc gcgatcggcg gtcttgtggg
     3841 cgcggtcctc ttcgcgctgg cgctcaaggc ctacacgccg tggctccctg tgcgcgaggc
     3901 cgccatcatg ggagcggtca taggagtcac cgggcagtgg ggcgatctct tcgagtccat
     3961 ggttaagcgc gacctgagac gcaaggattc ggggcggctc ctgccggggc acggcggcgt
     4021 gctcgaccgc ttcgacagcc tgctgttcgt gggagcggcg acctactggc tcagcgtcct
     4081 cttgcttggc gatctggtgc aggggggtgg gg
//
LOCUS       DASRMJ010000253         5474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_422460, whole genome shotgun sequence.
ACCESSION   DASRMJ010000253 DASRMJ010000000
VERSION     DASRMJ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422460"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1081
                     /locus_tag="VFD74_06990"
     CDS             302..1081
                     /locus_tag="VFD74_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZK49327.1"
                     /translation="MNKRHRIQFPAVDVRELCQDEVYFYLLDEGSREKIRLHDYDRIF
                     SIPGLYEQVVYERLRCQSPATVAKVLRYSVAQSSQQLNELRVLDLGAGNGIVGEELKK
                     QGVSRLVGVDIIPEARAATDRDRPGIYDAYYVMDFCDLTDDDRGELNSWAFDCLVSVA
                     ALGFSDIPAHAFLEAFNMISKGGWIAFNVKETLLDNSDDSGFSRLIRELIFSEYLDLY
                     HLQRYRHRYSIEGEPLYYFALGGKKIGDVTPSFADSVAPSA"
     gene            1252..1854
                     /locus_tag="VFD74_06995"
     CDS             1252..1854
                     /locus_tag="VFD74_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49328.1"
                     /translation="MRAVVCTGYGPSDVLELQQVEKPVPKDHEVLIKVHATTVHRGGL
                     TADAARELCAPGLRAALERAGLPPVIPVGACVDNVRTMFLWLRLEEAAGTSLAALPLF
                     YVGAEPGNEKALGMGMAFLAHGVNVLTGFPIPVPVPSAEHIPGGDTDDMRRVANPVAD
                     FFGQDAAGLLGARIFVEPAPELAAGAIHMDFRRKRRALGW"
     gene            complement(1963..3534)
                     /gene="trpA"
                     /locus_tag="VFD74_07000"
     CDS             complement(1963..3534)
                     /gene="trpA"
                     /locus_tag="VFD74_07000"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZK49329.1"
                     /translation="MTRVKICGLTNRDDALRAVECGADALGFVFAPCSRRRADPPTVA
                     RIVGDVPEGVTTVGVFQDQPIDVVRDIMLSCRLDVAQLHGSEDNTYMERLALPVLKAV
                     GLLGPQDLVSLADYQTPGIFLLDAAHVEMSADGGRLTVTGGTGASFDWSWAASARRFG
                     RIVLSGGLRPDNVAEAVMTARPWAVDTASGTECEPGRKDPEKLRSFVQRAKKSGAELS
                     EGEVSPGVGSPGLVDGGSSWGCIVRTRASVRVEGSGGAAVGRLEAVLRARRAAGGKSF
                     VPFFLGGYPDRDTFRAVLLEAERAGVDAIEVGVPSPNPALDGPVIRRASREALARGNS
                     SRTVLADIAAAREAGLTVPVVLMSYRDVFLRADGQSLVIDAQESGVDGLLIVDSGELD
                     AGAPPPVTPARALETVALVSPNTPERGYPSVLEWARGFVYCVSVAGGTGGEAASVATA
                     REMVRRVRCHGDIPALVGFGIAGPKAAREIAAVADGIIVGSALLARVGDNRGSTAVSA
                     VGRLLREMRQAVDHS"
     gene            complement(3531..4412)
                     /locus_tag="VFD74_07005"
     CDS             complement(3531..4412)
                     /locus_tag="VFD74_07005"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZK49330.1"
                     /translation="MSVLQRIVRDRRMRIELDKAAGPLSRQPSTPHVKGRAERFFAEL
                     ENRRPAATAHPGHGATGHALIAEIKRRSPSKGDLRPELDPVSLALAYQRAGACAISVL
                     TEPDHFGGSFEDLAAVSRAVEIAVLCKDFVVDPSQINRARAAGADLVLLMVAVLGRET
                     GRFVSLARCAGLEPLVEVHTGPELDLALAAGARLVGVNNRDLRTLSVDLDVCRSLLPR
                     IPTRIHAVAESGMATAEDLEDLALHGARAFLVGEALVTARDPGAALRRLARGEAPILE
                     APVSISETGSPSYRNRS"
     gene            complement(4409..5443)
                     /gene="trpD"
                     /locus_tag="VFD74_07010"
     CDS             complement(4409..5443)
                     /gene="trpD"
                     /locus_tag="VFD74_07010"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZK49331.1"
                     /translation="MLKPLIGKLVDGTHLSEVETMDAMEHIMGGQASDGQVGAFLAAL
                     RLKGETTDELVGAARVMRRHATPLTPPIAGLVDTCGTGGDASRSFNISTAAAFVLAGA
                     GLRVAKHGNRAVSSHCGSADVLQALGVCIEVDMETTRACIEEIGVGFLYAPMVHAAMR
                     HVAPLRRDIGIRTMFNLLGPLTNPAGVAHQVVGVSRPEHVRLLAEALLRLGTRRTLVV
                     HGFDGLDEVTISAPTLVAEVCDGAVTEYVVAPEDAGLERAPLETIRGGDERVNADIIR
                     RILAGERSPRRDVVLLNAAAALFVSGEARDLRAGTRLATLSIDSGAAREKLDHLAHRT
                     NRAHVARATT"
BASE COUNT          928 a         1810 c         1780 g          956 t
ORIGIN      
        1 gaatagatgt acccctcacc ccgtgaacgc ctcccaacgg gccagcacga tggaagcatg
       61 gtgttgaaca ggggcggcca cggcgtccgc agcccacttg acggttggtg gggctgccta
      121 tactgtctgg gtatccacga gacgcggtct cgcctcagcg ggactcgtgt cttgctggcg
      181 ggcgtttgcg ggttcgtatc ccattgctgc tttgttgata cacagacagc cgggcgctgt
      241 gtcggtggag ttatgcagac tggatattgg ttgttaggct gacagttcaa caggaggcga
      301 agtgaacaaa aggcatcgga tccaattccc ggcggtagat gtccgcgagc tatgtcaaga
      361 cgaagtctac ttctacctcc tcgatgaagg ttccagggag aagatccggc tgcacgacta
      421 cgatcgcatc ttctcgattc ccggcttgta tgagcaagtc gtctatgaac gcctgagatg
      481 ccaatcgcca gccaccgtcg cgaaggtgct ccgctactcg gtcgcacagt cgtcccagca
      541 gctgaatgaa ctgcgcgtac tcgacctagg cgccggaaat ggaatcgttg gcgaagagtt
      601 gaagaagcaa ggcgtctcca ggcttgttgg cgtggacatc atccccgagg cgcgagctgc
      661 aacggaccgc gatcgcccag gcatctacga cgcctactac gtcatggact tctgcgatct
      721 caccgacgat gaccgaggcg aactgaactc gtgggcattc gactgcctgg tttcagtcgc
      781 agcactcgga ttcagcgata ttccagccca cgccttcttg gaagccttca acatgatttc
      841 aaagggcggt tggatcgcgt tcaacgtcaa ggagaccctt ttagataaca gcgacgactc
      901 cggcttctcc cggctgattc gggaattgat cttttcggaa tacctggacc tctatcatct
      961 gcagcgctat cggcatcgct actcgatcga gggcgaaccg ctctactact tcgccttggg
     1021 cggcaagaag atcggagacg tcacgccctc ctttgcggat tcggtagcgc cgtcagccta
     1081 acaaggcgct gcagctgacg tggcacagcg cgtttcgatt ggggtctggt agtattcggg
     1141 cgtcaacctt gggtgcctca gcggcctccg gcggcctgtg ccacgcagct gagcgccggt
     1201 ccgttcgatg gggaacgggc aggcagatcc tctggctgaa gggatccagt gatgcgggca
     1261 gtcgtgtgca cgggatacgg tccttccgat gttcttgaac tccagcaggt agagaagcct
     1321 gttccaaagg atcacgaggt gctgatcaag gtgcatgcga cgacggtgca ccgtggggga
     1381 ttgacggccg acgcggcgcg ggagctgtgc gcccccggac tgcgcgccgc tctggagcgg
     1441 gccggactgc ctcccgtcat ccccgtgggg gcctgcgtgg acaatgtacg cacgatgttc
     1501 ctctggctgc ggctggagga agcggccggc acgtcccttg cggccctgcc cctcttctat
     1561 gttggcgccg aaccgggaaa cgaaaaggcg ctcggcatgg ggatggcctt tctcgcgcac
     1621 ggcgtgaacg tgctcacggg gttcccgatc ccggtgccgg tgccgtctgc ggagcacatc
     1681 ccgggaggcg acaccgacga catgaggcgg gtcgcgaacc cggtcgccga cttcttcggc
     1741 caagatgccg caggcctgct cggcgcccgg attttcgtgg agccggcgcc tgagctcgcg
     1801 gccggggcca tccatatgga cttcagacgc aagcggcggg ctctgggctg gtagggggca
     1861 tcacgcctcc cgccgagtgg gccgaagcgg caacgctggc cctcccgcat cggcacgggc
     1921 cgacaccatc acactcacga gtcggcactc tgggcggctg cctcacgagt ggtcgaccgc
     1981 ctgtctcatc tcgcgcagaa gacgccccac ggcactgacg gcggtcgagc cgcggttgtc
     2041 acccacgcgc gcgagaagcg cgcttccgac gatgatgccg tccgcgaccg cggcgatctc
     2101 ccgggctgcc ttcggcccgg ctatgccaaa gccgaccagc gcggggatgt caccgtggca
     2161 ccgcacgcgg cgcaccatct ccctggcggt cgccactgat gcagcctccc caccggtgcc
     2221 gcctgcgacc gacacgcagt agacgaaacc ccgggcccat tcaagcaccg acgggtaccc
     2281 gcgttccggg gtgttgggac tgaccagcgc cacggtctca agtgcacgtg cgggagtcac
     2341 gggtggcggg gcgccggcgt ccagttctcc ggagtcgacg atcaggagcc cgtcaacgcc
     2401 gctctcttgg gcgtcgatta ccaagctctg tccgtcagct cgaaggaaga catcacgata
     2461 gctcatgaga accacgggta cggtcagccc ggcttcccgt gccgcggcta tgtctgcgag
     2521 gacggttctg ctgctgttcc ctcgggcgag cgcctcgcgg gacgcccgtc ggatcacggg
     2581 gccatccaag gcaggattgg gactggggac accgacctcg atcgcatcca cgcctgcccg
     2641 ctctgcctcg aggagcaccg cgcggaaggt gtcccgatca gggtacccgc ccaggaagaa
     2701 gggcacgaag gatttcccgc ccgcagcgcg ccgggcgcga agcaccgctt ccaagcggcc
     2761 gacggccgcg ccgccggacc cttcgacgcg gacggacgcc ctggtcctga cgatacaacc
     2821 ccaactcgac ccaccgtcga caaggccggg agaacccacc ccgggtgaga cttccccctc
     2881 actgagttcg gctccactct tcttcgcccg ttgcacgaag gagcgaagct tctcaggatc
     2941 cttgcgtccc ggctcgcact ccgtgccgga tgctgtgtcg accgcccagg gtcgggctgt
     3001 catcactgct tcggccacat tgtccggtcg caggccgccg ctcaacacga ttcgtccgaa
     3061 tcggcgggcc gaggcggccc aggaccagtc gaacgacgcc cctgtgccgc ccgtgaccgt
     3121 cagccggccc ccatccgctg acatttccac gtgggcggca tcaagaagga agattcctgg
     3181 tgtctggtag tcagccaggg agacaaggtc ttggggcccg agcagtccca ccgccttcag
     3241 cacggggaga gccagtctct ccatgtaggt attgtcttcg ctcccgtgaa gctgagccac
     3301 atccagccga cagctgagca tgatgtcgcg cacgacgtcg atgggttggt cctggaagac
     3361 acccaccgtg gtgactcctt cgggaacgtc cccgacgata cgcgcgaccg tcggggggtc
     3421 ggcccgcctc cgactgcagg gggcgaagac gaaacccaac gcgtccgccc cgcactcgac
     3481 cgccctcagc gcgtcgtcac gattggtgag gccgcagatc ttgaccctcg tcatgaccga
     3541 tttcgatagg acggcgaccc tgtctcagat atcgacacgg gtgcttcaag gattggcgcc
     3601 tcccctcgcg caagtcgcct cagcgcggct cctgggtccc gtgccgtcac cagggcctct
     3661 cccacgagga aggcccgggc accgtgaagg gcgaggtctt caaggtcctc cgccgtcgcc
     3721 atgccgctct cggccaccgc gtgaatccgg gtgggtatgc ggggcagaag gcttcggcag
     3781 acgtcgagat ctacggaaag cgtgcggagg tccctgttgt tgacgcccac gagtcgagcg
     3841 ccagccgcga gcgcaaggtc gagctcgggg ccggtatgca cctcaaccag tggttccaag
     3901 ccggcgcacc gggcaagcga gacgaaacgc cccgtctccc gtccgagcac cgcgaccatg
     3961 agcaacacca ggtcggcgcc ggcggcccga gcgcgattga tctgcgaagg gtccacgacg
     4021 aagtccttgc acaggacggc gatctctacc gcccgactga cggcggctag atcctcgaaa
     4081 cttcctccga agtgatctgg ttcggtgagc accgagatcg cgcaggctcc cgcccgctgg
     4141 taggcgaggg cgagggatac ggggtcgagt tcggggcgca ggtcgccctt cgaagggctg
     4201 cggcgtttga tctccgcgat cagcgcgtgg ccggttgctc cgtgccccgg gtgggccgtc
     4261 gcagcgggtc ttcgattctc gagctcggcg aagaacctct cggccctgcc tttgacgtga
     4321 ggggtggacg gctgcctcga cagtggcccc gcggccttgt ccagttctat gcgcatgcgc
     4381 cggtctcgga cgatacgttg caagacgctc atgtcgtcgc ccgcgccacg tgcgcgcgat
     4441 tcgtcctgtg ggccaagtga tcgagcttct cccgggccgc gcccgagtcg atcgagagcg
     4501 tcgccaagcg ggttcccgcg cgcaggtcgc gcgcctcccc ggacacgaag agagcggcgg
     4561 ccgcgttcag caacaccaca tcccgccgcg ggctgcgctc ccctgcgagg attcgtcgga
     4621 tgatgtccgc gttgacgcgc tcgtcaccgc ctcggatggt ctcgagcggt gcccgttcga
     4681 gaccagcgtc ctcgggcgcg accacgtact cggtcaccgc tccgtcgcac acctctgcga
     4741 caagtgtggg ggcagatatc gtcacttcgt caagcccgtc aaatccgtgc acgaccaggg
     4801 tccggcgtgt tcccagacgg agcagggctt cggcgagcag tcgcacgtgc tcgggccggg
     4861 agaccccgac gacctggtga gcaacgccgg cggggttcgt caacgggccg aggaggttga
     4921 acatcgtgcg gatgccgatg tcgcgtcgca gcggcgcgac gtggcgcatg gctgcgtgga
     4981 ccatcggcgc gtagaggaac cccacgccga tctcttcgat acaggcgcgg gtcgtctcca
     5041 tgtcaacctc aatacagact ccgagtgcct gcagcacatc ggcgctcccg caatggctcg
     5101 agacagcccg gttgccgtgc ttggccacgc gaaggcccgc tcccgcgagc acgaacgcag
     5161 ctgctgtgga gatgttgaag ctgcgagagg cgtcgcctcc tgtgccgcac gtgtcgacga
     5221 ggccggcgat gggtggggta agcggggtgg cgtgacggcg catgacgcgg gccgctccca
     5281 cgagctcgtc ggtggtctcc cctttgagcc ttagggcggc gaggaacgcc ccaacctgtc
     5341 cgtcggaagc ctgcccaccc atgatgtgct ccatggcgtc catcgtctcc acctcactga
     5401 ggtgggtacc gtcgaccagc ttgccgatca gcggtttgag catagttcct ccttggggtg
     5461 cgcccctgcc gggc
//
LOCUS       DASRMJ010000254         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_493403, whole genome shotgun sequence.
ACCESSION   DASRMJ010000254 DASRMJ010000000
VERSION     DASRMJ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493403"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VFD74_07015"
     CDS             <1..1477
                     /locus_tag="VFD74_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HZK49332.1"
                     /translation="PIPFSFSPAGDRNAEVIRRLSEGDEVVLIDRTKKPVALLKVHDV
                     YELDREARGANLFGTSDPAAHPGVAAIFRRMARYALAGPIELIERPSWGAFERHRLTP
                     KDTYDLFYRQRGYQKVAGFVTGANPPHVGHEHMHRVALESVDAILLLPQVEMERPEYI
                     RPYHRLMALEALTDVYYPPYRIVLSALRTNYLFAGPREALLHAVIMRNYGCTHALIGR
                     DHAGVGDMYDLYAGQRVFGQYSPADLGIEPIFFSEIFYCTRCRASATERTCPHDTRYR
                     LQISGTGIREILRRGYLPPKEICRPEVAHIAIQGVEPPTTDEHGQSVYPVGQTIHSLF
                     PFYEVATRLGGYLRPRAVPTETLGDRDLEAALLDVRAHADGIYADVFQEVAAAAEVER
                     SLAERWRVEAQEILVDHQSEVVSRFREQMQGAKEGIDQLTGSTSSEVADDLQAARQIL
                     SEHPRPLHPEQARHRVLPRERQPEGSGVVKATSDDVEAASE"
     gene            1474..1887
                     /locus_tag="VFD74_07020"
     CDS             1474..1887
                     /locus_tag="VFD74_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK49333.1"
                     /translation="MKDIRAFDFTTAEYPRVGYDDNVLDALGYMNAGQRDYAMVVEDG
                     DIVGVLEQAEVVFAYGRGELSAETRVAQIARDLPWIDAGATFDDVVQFMAALDIFQVC
                     IENTVLDDFMLLRAIWTERLAMAREFREYEAPEAE"
     gene            1887..2327
                     /locus_tag="VFD74_07025"
     CDS             1887..2327
                     /locus_tag="VFD74_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK49334.1"
                     /translation="MAASTVGDVCTTSFTTVPRRRRVVEVARMIAGRWLSCVIVVEGG
                     RPVGIVTKHTLVRDVLAAGYTGMEMTAGDIMRSPIVSVDAGASPEDAARLMAATSIRY
                     LAVTRDGLLVGLLSHYHLARLLPDLIGRAGPATVPPGGADPQPS"
     gene            complement(2266..>2695)
                     /locus_tag="VFD74_07030"
     CDS             complement(2266..>2695)
                     /locus_tag="VFD74_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZK49335.1"
                     /translation="FRVPFATSPFRYQNRDELWELLERHNLFMTFDTTHFGTTGESLG
                     GCFAQFRERVRTIHLSNFRSRDFEEHTPLHDGDLDLGGFLRRVKSTGYDGLVTLEVRP
                     QSFEQDPAGPGEALRRSVEWFRKAVGPLHREARSQAPPAR"
BASE COUNT          457 a          874 c          913 g          451 t
ORIGIN      
        1 gcccatcccc ttctcgttct cgccggcggg tgatcggaac gccgaggtca tccgcaggct
       61 ctccgagggc gacgaggttg tcctgatcga tcggacgaag aaacctgtcg cgctgctcaa
      121 ggtgcacgac gtgtacgaac ttgacaggga ggcgcgaggt gccaatctgt tcggtacgag
      181 cgacccggcg gcgcaccccg gcgtggctgc catcttcagg cggatggcgc gatacgccct
      241 cgcaggcccc atcgaactga tcgagcgccc gagctggggg gcgttcgagc gccaccgcct
      301 caccccgaag gacacctatg acctgttcta ccggcagcgg ggatatcaga aggtcgccgg
      361 gttcgtgacg ggagccaatc caccgcatgt cgggcacgag cacatgcatc gggtcgcgct
      421 cgagtcggtg gacgccatcc tgcttcttcc gcaggtggag atggagcgac cggagtacat
      481 ccgtccgtac catcggctca tggctttgga ggcgctcaca gacgtctact atccgcccta
      541 ccgcatcgta ctgtcggcgc tgcggacgaa ctacctcttc gccgggccgc gggaggccct
      601 gctgcacgct gtgatcatgc gcaactatgg gtgcacccac gcactgatag gccgcgacca
      661 cgcgggcgtt ggtgacatgt atgacctcta tgccggtcag cgggtgttcg gccaatacag
      721 cccggcagac ctcggcatcg aacctatctt cttctcggag atcttctact gcacccgatg
      781 ccgagcctcg gcgacggaac gcacctgtcc gcacgacacg cgctatcggc tgcagatctc
      841 gggcaccggc atccgcgaga tcctgaggag gggctatctg ccacccaagg agatctgccg
      901 gcccgaggtg gcgcacatcg ccatccaggg cgtggagccg cccaccaccg acgagcacgg
      961 ccagagcgtc tatccggtcg gccagaccat ccactcgttg ttccccttct acgaggtcgc
     1021 cacccgactc gggggctacc tgaggccgcg tgccgtcccc acagagacgc tcggagacag
     1081 ggacctggag gccgcactac tggacgtgcg tgctcacgcc gacggcatct acgccgacgt
     1141 gttccaggaa gtggccgccg ccgccgaggt cgagcgctcg ctcgccgagc gctggcgggt
     1201 ggaagcgcag gagatcctgg tggaccatca gtccgaggtg gtctcacggt tccgtgagca
     1261 gatgcagggt gcgaaggagg gcatcgacca actcaccgga tcgacctcgt cggaggtggc
     1321 ggacgatctg caggcggctc gccagatcct ctccgagcac ccgcgtcctc tgcacccgga
     1381 gcaggcccgg caccgggtgt tgccgcgcga gcggcagccg gaaggatccg gcgtggtcaa
     1441 ggccacctcc gacgatgtgg aggcggcctc agaatgaaag acatccgggc cttcgacttc
     1501 accacggccg aataccccag ggtcggctac gacgacaacg tgctcgatgc cctcggatac
     1561 atgaacgccg gccagcgcga ctatgcgatg gtggtggagg acggggacat cgtcggcgta
     1621 ctggagcagg cggaagtggt cttcgcctac gggcgggggg aactctcggc cgaaaccagg
     1681 gtggcgcaga tcgcccgcga tctgccctgg atcgacgcgg gcgccacctt cgacgatgtg
     1741 gtgcagttca tggcggccct cgacatcttc caggtctgta tcgagaacac ggtgctcgac
     1801 gatttcatgc tactgcgggc catctggact gaacggctgg ccatggctcg tgagttccgc
     1861 gaatacgaag ctcccgaggc ggagtgatgg ctgcgtcgac ggtcggagac gtctgcacca
     1921 cgagcttcac caccgtgccg cgccgccggc gggtggtcga ggtggcgcgc atgatcgccg
     1981 gtcgctggct ctcgtgcgtg atcgtggtgg agggcggccg cccggtgggc atcgtgacga
     2041 aacacacgtt ggtgcgtgac gtccttgccg ccggttacac aggcatggag atgacggcgg
     2101 gggacatcat gcgctcgccc atagtgtcgg tggatgccgg ggccagtccg gaggacgccg
     2161 cccggctcat ggcggccaca tccatccgct atctggcggt gacgagggac ggtctactgg
     2221 tggggctgct ctcgcactac cacctggccc gactgctgcc cgatctcatc gggcgggcgg
     2281 ggcctgcgac cgtgcctccc ggtggagcgg acccacagcc ttcctgaacc attcgacgct
     2341 cctgcgcagg gcctcgccgg ggccggcggg gtcctgctcg aaagactgag ggcgcacttc
     2401 caaggtcacg aggccgtcat agcccgtcga cttcactcgg cgcaggaatc cgcccagatc
     2461 gaggtcgccg tcgtgtagcg gcgtgtgctc ctcgaagtcc ctgctgcgga agttcgagag
     2521 atggatcgtg cgtacgcgct ccctgaactg cgcgaagcag ccgccgaggc tctcgccggt
     2581 cgtcccgaag tgggtggtgt cgaaggtcat gaacaggttg tgccgttcga ggagctccca
     2641 cagctcgtca cggttctggt aacggaaggg cgaggtggcg aaaggcactc tgaag
//
LOCUS       DASRMJ010000255         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_493488, whole genome shotgun sequence.
ACCESSION   DASRMJ010000255 DASRMJ010000000
VERSION     DASRMJ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493488"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFD74_07035"
     CDS             <1..214
                     /locus_tag="VFD74_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49336.1"
                     /translation="AWIVGGATFAGGRHRRGKPGCESESISQLDQQASPGMRDDPFGV
                     RPDFYGLARRVSGRGHRSPRLSQNRT"
     gene            complement(225..1502)
                     /gene="ltrA"
                     /locus_tag="VFD74_07040"
     CDS             complement(225..1502)
                     /gene="ltrA"
                     /locus_tag="VFD74_07040"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZK49337.1"
                     /translation="MTEPKSKVQKSFSIDKRVVWEAWKQVKANRGAPGVDEESVAEFG
                     RDLQGNLYKLWNRMSSGTYMPPPVKAVQIPKKDGRGVRTLGVPTVADRVAQTVAKLYL
                     EPQVEPIFHSDSYGYRPDRSALDAVGVCRERCWKSDWVIDMDYRSFFDSVDHDLILKA
                     VAAHTKERWVLLYVERWLKAPLDEGDGNPEARDRGTPQGSAISPLLANLYLHYAFDSW
                     MAREFPAVPFERYADDVVVHCQSERQARFVLGEIEKRMAACLLELNQDKTHIVYCKDS
                     NRSGSHEHEPFDFLGYTFRPRKAKNRKGELFCSFLPAVSDEAAKQIRRTIRRWRLHLW
                     SGTPLTKIARACNAVVRGWINYYGRFYPTRLIESLQCIDKYLVRWAMQKYKRLRGHHK
                     RAWKFLADVCAREPELFAHWRLTQTSGRKAGAV"
     gene            2252..2458
                     /locus_tag="VFD74_07045"
     CDS             2252..2458
                     /locus_tag="VFD74_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZK49338.1"
                     /translation="MLATIERGDWLRLERLLAPDVHWTTALEEHLHGPAEVIALLSTD
                     PPPAPPAFHDLHDGLIVRWIDTPG"
BASE COUNT          395 a          860 c          751 g          521 t
ORIGIN      
        1 tgcctggatc gtgggcggag cgacgttcgc gggtgggcgc catcgccgcg gaaagcccgg
       61 ctgtgagtcc gagtcgatct cccagctcga ccagcaggcg agccccggca tgagagacga
      121 tcccttcggc gtccgccctg acttctacgg tttggcgcgt cgggtgagtg gccgagggca
      181 tcgcagccct cggctctcgc agaaccggac gtgacagtct cccgtcatac ggctcccgcc
      241 ttccggccgc tggtctgggt caacctccaa tgggcgaaaa gctccggctc gcgcgcgcac
      301 acgtccgcca ggaacttcca cgctcgtttg tggtgacctc gaagtcgctt gtacttctgc
      361 attgcccagc gcaccaggta cttgtcgatg cactggaggg actcgatcaa cctggtcggg
      421 tagaaccgcc cgtagtagtt gatccagcct cgcacaacgg cgttacacgc tcgtgcgatc
      481 tttgtcaggg gtgtcccact ccatagatga agcctccatc tgcggattgt tcggcgtatc
      541 tgcttggcgg cttcgtcgct gaccgccggg aggaagctgc aaaacagctc gcccttccgg
      601 tttttggcct tgcggggacg aaacgtgtac ccgaggaagt caaagggctc gtgctcgtga
      661 gagccgctgc ggtttgagtc cttgcagtag acgatgtgcg tcttgtcctg gtttagctcc
      721 aggaggcaag ccgccatccg cttctcgatc tcgccgagca cgaaccgcgc ctggcgctca
      781 ctttggcaat gcacgacaac gtcgtctgcg tagcgctcga aggggacggc cgggaactcc
      841 ctggccatcc atgaatcaaa cgcgtagtgc aggtagaggt tggcgagcag cggggagatc
      901 gcggaccctt gcggggtgcc gcgatcacgc gcttcggggt tgccgtcccc ttcgtcaagc
      961 ggggccttca gccagcgctc gacgtagagg aggacccacc gctccttggt gtgggcggct
     1021 accgctttca gtatgaggtc gtggtcgacg gagtcgaaga aggaacggta atccatgtcg
     1081 atcacccagt ccgacttcca acaccgttct cgacacaccc caaccgcgtc aagggccgag
     1141 cgatccggtc ggtagccgta ggagtctgag tggaatatcg gctccacctg cggctccagg
     1201 tacagcttgg cgaccgtctg cgcgaccctg tctgcgactg taggcacacc gagggttctg
     1261 acccctcgcc catccttctt cggtatctgc accgccttca ccggcggcgg catgtaggta
     1321 cctgaggaca ttcggttcca gagcttgtag aggttccctt gcagatccct cccgaactcg
     1381 gcaaccgact cctcatcgac gcccggagcg ccccggttgg ccttgacttg cttccaagcc
     1441 tcccagacca ccctcttgtc aatgctgaac gacttctgga ccttcgactt tggctcggtc
     1501 atcggctcct cccacaaact tcgtggttga ccgtcaacgt cgcctggatg gcccgcccgc
     1561 ttcgctccaa cccgattacc gggccttcgt cgctactacc aggcggtccg cccctgtgcc
     1621 tcgcttcagg attgggcccc tcgcggtctc ttccgcttgg ggccttccct gggagggccg
     1681 gccggaggtc tggtgaccat gagccgatct cccgcgagac gacaggttcc catgttccgc
     1741 acaagagcct gacccgcgct cgcgccacct ccatgccgga caccgcctgg gcagtaggca
     1801 ggtgcccccc aggctcatcc caggccccac aaaatgacct ggtttcgatg tcgtctgatg
     1861 aatttcgaca cgtctgcggt ggttcgcttt cgctcgcctt catcgggtca tacctgacgt
     1921 gctcgacggc acgccttttc ccgcaacgct cagcacgaac ggctcttgac cgacgcacct
     1981 tgcggtggtt tgtggcctcc tcctgcaagg cgaccacgga ggggctgcct ccatctcctt
     2041 tgcagcgccg cgtcaggagc gcgccgagcc cttcctgcgg tgcttgggct tggccctctt
     2101 cctgttgcgt tcatggcgca ctttgtgtct tcaccttccg ggtgtcctcc tgggtcttgg
     2161 ggaatgggtc ggcaaaccgc attctcaaga cccaagagga cgttcccggg cggtttcctc
     2221 aggtgcttac tgccgaatcc gggttagcaa cgtgctcgcc acgatcgagc gaggggactg
     2281 gctgcgcctc gagcgcctgc tcgccccaga cgtccactgg acgactgcgc tggaggagca
     2341 tctgcacggt cccgcggagg tgatcgccct cctctcgacc gaccctcccc cagcccctcc
     2401 ggccttccac gatttgcacg acggcctgat cgtccgctgg atcgacaccc ccggctgacg
     2461 ctgcaggcgc gtttacgccc ggcgttttcc acttgctaaa gcggttccgc ggaaccgtgc
     2521 caccctc
//
LOCUS       DASRMJ010000256         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_100686, whole genome shotgun sequence.
ACCESSION   DASRMJ010000256 DASRMJ010000000
VERSION     DASRMJ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100686"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFD74_07050"
     CDS             complement(<1..314)
                     /locus_tag="VFD74_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49339.1"
                     /translation="MGRPATRGLKWRRRISNLLILGGLLVLLYPVGTWAVTWWEQRAL
                     QQALVASHPDLAEDAGAALQDDAVLVPLDFGDASAEHLTVKEQEQKAESIALTRALAR
                     VRA"
     gene            complement(323..1138)
                     /locus_tag="VFD74_07055"
     CDS             complement(323..1138)
                     /locus_tag="VFD74_07055"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HZK49340.1"
                     /translation="MIDTHSHVLPGLDDGASDMSEAISLARSAVDDGVREVVCTPHAR
                     ERGDRSLTMGRGALTELESALSEAQIPLRLHLGYELSFSFASSLDVSELNDLTLGPRS
                     RAILVELPYSGWPLGAQDAVFRWRLQGLLPVLAHPERNDRVQRDPAVLEELLRIGAVA
                     QGTLPSLLGTFGSASRKTFLRLLAAGRFAMLASDAHYGSGRPASMAEGLRLMRAWAPG
                     ADVQSLTVDNPARLLRGEPLRALPPVRVRRAWEKYLRRTEKSTSARPYSAGLS"
     gene            1362..2738
                     /locus_tag="VFD74_07060"
     CDS             1362..2738
                     /locus_tag="VFD74_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49341.1"
                     /translation="MPSFDTISEWVLANLYVVGTATGAVVLAAILIVLLAKRRGRDAH
                     GGTTMLEAKPVYPRLDLPTSVPAGTGRDEFEYRDASADRDASAVWDTSAYRDASSALG
                     DAVTHTPGGGAAEAAGSGSAGAEAALPEGADDLPYMPPPNPHVNHVRDIVQDLIQGPG
                     DITGAELRRLDLIRPERVLEVAAELDAGLGGRGKEALRTRLAQISRYAELLMPAEAAP
                     DVFAADFGRPVPATPDGSVSQPHDDLVGAAAERSYGYGAELMLDPELSLLADEPEPAV
                     APAPTPVIGEAAFIDAMPEPTAEDRPPQDQEEEAWQPTVVDKTADDWRVEAITEDEGP
                     WQPAAPSDGADPWAAAVAEEAGTWGIAETEEAGAWKVADTKGADAVPAVDEAPTPDLL
                     MSLGAAELGRLFASTDDKSLKLSIIDALETTWTPETMTAIQVCLDDADPEVQVHALEA
                     AERLLGRL"
     gene            complement(2757..>3472)
                     /locus_tag="VFD74_07065"
     CDS             complement(2757..>3472)
                     /locus_tag="VFD74_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZK49342.1"
                     /translation="LQAFAPESYGVVSEEAGVIPGPKGSALVVIDPVDGSLNAKRGLP
                     PYCAAFALARGGTLGDVELGYVADYSRTGVHAALRGAGFVSSRGTAQVASGMDRAAVH
                     SASCEDARWDRQVEVVLLEAGRPHGHTFAYSDLARLAGAGAGPEMRIRQIGSLALCLC
                     HLSAGTADVLFAPVPSRSVDIAGGLLMVAESGGGAVSLDGSNLLAQPLDLAKRAPFVA
                     WRHGLDSERVVHAARTILI"
BASE COUNT          624 a         1194 c         1180 g          474 t
ORIGIN      
        1 gccctgaccc gcgccagagc gcgggtcagg gcgatacttt cagccttctg ttcctgttct
       61 ttgacggtca ggtgttctgc gctcgcatcg ccgaagtcca gcggcacgag cacggcatcg
      121 tcctgcagcg ccgcgccggc atcctccgcc aagtcggggt gcgaggccac aagagcctgt
      181 tggagagcgc gctgctccca ccaggtcacc gcccacgtgc cgactggata cagcagaacc
      241 agcaatccac ccaagatgag cagattggat atgcgacgcc tccacttgag gccccgcgta
      301 gcgggtcttc ccaccggcgc tcctagctca ggcccgcgct gtaggggcgc gcggaggtcg
      361 atttttcagt cctgcgcaag tacttttccc aagccctgcg caccctaacc ggaggaagcg
      421 cacgcagtgg ctctccccgg agcaggcggg cggggttgtc cacggtcaga gactggacat
      481 cggcgcccgg agcccacgcg cgcatcagac gcagcccctc cgccatcgat gccgggcgcc
      541 ccgagccgta gtgcgcgtca ctcgccagca tggcaaagcg tccggcggcg agcaggcgga
      601 ggaaggtctt gcgcgaagca cttccaaagg tgccgagtag gctcggcagc gtgccctgcg
      661 ccaccgcgcc gatgcgcagg agttcctcga gcaccgcggg atcccgctgc acccggtcgt
      721 tccgttccgg atgcgccagc acaggaagca gaccctggag tctccatcgg aacaccgcgt
      781 cctgggcgcc cagaggccag cccgagtacg gcagctccac caggatcgcc cgcgaccgag
      841 ggccgagcgt cagatcgttg agctccgaca cgtcgagcga ggaggcgaag gagaacgaga
      901 gttcgtagcc aagatgaaga cggagtggga tctgggcctc cgagagggca ctctccaact
      961 cggtgagggc tccgcgaccc atggtcagcg accggtcgcc ccgttccctc gcgtgaggcg
     1021 tgcagacgac ctcccgcaca ccgtcgtcga cggcggagcg ggccaagctg atggcttcag
     1081 acatatcgga ggccccgtcg tccagaccgg gaagcacgtg cgagtgggtg tcgatcatgt
     1141 gtgggtaccc ctaagacgcc cagacggcgc cgattttcgg ttggcgtgct ctatgctaga
     1201 gcaaactcgc cgcttcgccc accctggcgg gatatggaag tcgcatatgc cggacatgga
     1261 taccgacttg agtaactcgg aagcgggacc gacgttctga accaggcgag accatcgacc
     1321 cgcggctggc cgacacgcag ggacggaagg acggaagaca gatgccgagc ttcgacacta
     1381 taagcgagtg ggtgctcgcc aacctctacg tggtgggtac ggccaccggc gccgtcgtcc
     1441 tggcggcgat cttgatcgtc ctgctcgcga agcgacgcgg gcgcgatgca cacggtggca
     1501 cgacgatgct cgaggccaag cctgtgtatc cccggctgga cctgccgaca tcggtgcccg
     1561 ccggcaccgg ccgagatgag ttcgagtatc gcgacgcgag tgccgatcgg gatgcgagcg
     1621 cggtctggga cacgagtgcc tatcgggacg cgagctcagc cctgggtgac gcggtcacgc
     1681 acacgcccgg cggaggcgcc gccgaggcgg ccggatccgg ctccgccggc gcagaagcag
     1741 ctctcccgga aggagccgac gacctgccct acatgccgcc accgaacccg cacgtcaacc
     1801 acgtccgcga catcgtccaa gacctgatcc agggaccggg agacatcacc ggcgccgagc
     1861 ttcgacggct ggatctcatt cgaccggaga gggtcttgga ggtggccgcc gagctggatg
     1921 ccggcctcgg cggccgaggc aaggaggccc tacggacccg tctggcgcag atctcgcggt
     1981 acgctgaact gctcatgccg gcagaagcgg cgccggacgt cttcgcggcg gatttcggca
     2041 gaccggtgcc cgcgacgccc gacggaagcg tctcccagcc ccacgacgac ctcgtcggag
     2101 ccgcagcgga gcggagctac gggtacggag ctgagttgat gctggacccc gagttgtccc
     2161 tgctggcgga cgagccggag cccgcggtcg cgcccgcgcc gacgcccgtg atcggggagg
     2221 cggctttcat cgacgccatg ccggagccga cggccgagga tcgcccgccc caagatcagg
     2281 aagaagaagc ctggcaaccc acggtggtgg acaagacagc cgacgactgg cgcgtcgaag
     2341 cgatcaccga ggacgagggg ccgtggcaac ccgccgcacc cagcgatggt gccgatccgt
     2401 gggcggccgc ggtggccgag gaagccggca catgggggat cgctgagacc gaggaagccg
     2461 gcgcgtggaa agtcgccgat accaaggggg ccgacgcagt cccggcggta gacgaggccc
     2521 ctacgcccga cctgctcatg tccctcggtg ccgcggaact gggccggctc ttcgcatcca
     2581 cagacgacaa gtccctgaag ctctcgatca tcgacgccct cgagaccacg tggacgccgg
     2641 agacgatgac cgctatccag gtttgtctcg atgacgcgga cccggaagtc caggtgcacg
     2701 ccctcgaagc cgcggagcgc cttctggggc ggctgtagcc gcggctcagc ggcggctcag
     2761 atcaggatcg tgcgcgccgc gtggaccacg cgctcgctgt cgagcccgtg cctccaggcc
     2821 acgaacgggg ccctcttcgc cagatctaag ggttgcgcga ggagattcga tccatcgagg
     2881 gaaacggccc ctccacccga ttcggccacc atcagaaggc cgccggcgat gtccaccgag
     2941 cgcgacggca ccggcgcgaa gagcacgtcc gcggtcccgg cgctgagatg gcacaggcag
     3001 agcgcgagag agccgatctg acggatcctc atctcgggtc cggcgcccgc gccggcgagg
     3061 cgggccagat cgctgtaggc gaaggtgtgc ccgtggggcc ggcccgcctc caagagcacc
     3121 acttcgacct ggcggtccca acgagcgtct tcacacgagg ccgagtggac cgcggcccga
     3181 tccatcccgg acgccacctg tgccgtcccg cgagacgaga cgaaaccggc tcccctcaga
     3241 gccgcgtgca cgccggtgcg agaatagtcg gcgacgtagc ccagctcgac atctcccaag
     3301 gtcccgccgc gggcgagggc gaacgcggcg cagtagggag ggagtccccg cttcgcattg
     3361 agactgccgt ccaccgggtc gatcaccacc agcgcgctac ccttggggcc gggtatcacg
     3421 cccgcctcct ccgacacgac accgtatgac tcaggcgcga aggcctgcaa ag
//
LOCUS       DASRMJ010000257         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_241062, whole genome shotgun sequence.
ACCESSION   DASRMJ010000257 DASRMJ010000000
VERSION     DASRMJ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241062"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFD74_07070"
     CDS             complement(<1..202)
                     /locus_tag="VFD74_07070"
                     /inference="COORDINATES: protein motif:HMM:NF045191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49343.1"
                     /translation="MATGSPKPSSSTVADLAGRGLDDLRKVADLLAARGRLARVRTSV
                     DPELELGGVARRFHGGKAVLFDK"
     gene            complement(202..1500)
                     /locus_tag="VFD74_07075"
     CDS             complement(202..1500)
                     /locus_tag="VFD74_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZK49344.1"
                     /translation="MNPTTVAVLGIVLMFVLMMLRVPVAFAMLLAGVIGNAYLLTTGV
                     SLHIMATNVWGQFASYSLSVVPLFVLMGQITFHSGVTERLYNAAHKWVGRLPGGLAAT
                     TILASMGFAAICGSNSATAATMGTVALPEMKRYKYDGALSTGAVAIGGTLGVVIPPSV
                     VLIIIAVQTEQSIVKLFLANIIPGVILTALLLATVFVVAFRNPTLGPPADSTTWKEKF
                     TSLTGVVEAIALFALVIGGLYAGWFTPTQAGAAGAFGAIIIGFARRTLSLKGLHLAIG
                     ETLRTSAMVVLLITGAVLFGKFLTVTRLPFDLAEWATGLPIPPIGILGVVLFIYLIGG
                     CLMDALGFLVATIPIFFPLAVALGYDPIWFTVMLTLVTTMGAVTPPVGVNVYIVSGLV
                     PDIPIQTIFRGVMVFLIAYVIVLLLLVVFPQLALFLPNSL"
     gene            complement(1507..2010)
                     /locus_tag="VFD74_07080"
     CDS             complement(1507..2010)
                     /locus_tag="VFD74_07080"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZK49345.1"
                     /translation="MHYLDKINEMINKGMAVVAGAALVCMMFVTVLDAFLRAFAKPLT
                     GTFEMVSWLAALTAAFSLGYTQLHKQHVSIDLLVKMFGVRTRSAIEAGVHLVSLVLFG
                     AVSYKMFIYAGTVRASGSLSETMKVVYYPWVYLVAIGCLGLVIALVLDFLHSLRECFP
                     RQTTGTW"
     gene            complement(2100..3002)
                     /locus_tag="VFD74_07085"
                     /pseudo
     CDS             complement(2100..3002)
                     /locus_tag="VFD74_07085"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          590 a          953 c         1107 g          514 t
ORIGIN      
        1 ccttgtcgaa gagcaccgcc ttcccgccgt ggaagcgacg ggccacgccg ccgagctcca
       61 actccgggtc taccgaggtg cggacgcgcg cgagcctgcc ccgggcggcg agcaggtccg
      121 ccaccttccg caggtcgtcg agtccgcggc cggcaagatc ggccacggtg gaagaggacg
      181 gcttcggcga tccggtggcc atcacagcga gttaggcagg aacagcgcca gttgcgggaa
      241 cacgaccagc agcagcagca cgatcacgta cgcgatcagg aagaccatca cgccacggaa
      301 gatggtctgg atggggatgt caggcaccaa gccgctcacg atgtacacgt tcacacccac
      361 cggcggagtc acggcgccca tggtggtcac cagcgtcagc atgacggtga accagatggg
      421 gtcgtagccg agggcgacgg ccagcgggaa gaagatcggg atggtggcca ccaggaagcc
      481 gagggcatcc atgagacagc cgcctatcaa gtagatgaac agcacgacgc cgagtatccc
      541 tatcggcggg atggggaggc cggtggccca ttcggccaga tcgaacggga ggcgcgtgac
      601 cgtgaggaac ttgccgaaca gcaccgcgcc ggtgatcaac agcaccacca tggccgaggt
      661 gcgcagggtc tcgccgatgg ccaagtgcaa tcccttgagc gacaaggtgc ggcgggcgaa
      721 accgatgatg atggctccga acgcgccggc cgctcccgcc tgggtgggcg tgaaccaacc
      781 ggcgtagagg ccgccgatca ctagggcgaa gagggcgatg gcctcgacga cgcccgtaag
      841 cgacgtgaac ttctccttcc aggtggtgga gtcggcgggc gggcccaggg tcgggttgcg
      901 gaaggcgacc acgaagacgg tcgcgagtag cagggcggtg aggatgactc cggggatgat
      961 gttcgccagg aacagcttca cgatcgactg ttcagtctgg actgcgatga tgatgagcac
     1021 cacgctgggc gggatgacca cgcccagggt cccgccgatg gcgaccgcgc ccgtgctgag
     1081 cgccccgtcg tacttgtagc gtttcatctc gggcagggcg accgtgccca tggtggcagc
     1141 cgtggccgag ttcgatccgc agatggcggc gaagcccatg ctcgcaagga tcgtcgtcgc
     1201 cgcgagacct ccgggaaggc gcccgaccca cttgtgggcg gcgttgtaga ggcgctcggt
     1261 caccccggag tggaacgtga tctggcccat gaggacgaag agcggcacca cgctcaggct
     1321 gtaggacgcg aactggcccc atacgttggt ggccatgata tggagcgaga cacccgtggt
     1381 gagcaggtac gcattaccga tgacgcccgc gagcagcatt gcaaaggcca cggggacgcg
     1441 cagcatcatg aggacgaaca tgaggacgat gccgaggacg gcgactgtag tggggttcat
     1501 atcggcctac caggtgccgg tggtttgacg ggggaagcac tcgcggagag agtgcaggaa
     1561 gtccaagacg agtgcgatca ccaacccgag gcagccgatg gcgaccaagt agacccacgg
     1621 gtagtagacc accttcatgg tctccgatag ggagccgctc gccctcacag tgcccgcata
     1681 gatgaacatc ttgtagctga cggccccgaa caacaccagg gagaccagat ggacgccggc
     1741 ctcgatggcg gagcgggtcc gaactccgaa catcttcaca agaagatcga tcgacacatg
     1801 ctgtttgtgc agctgggtgt agccgagcga gaatgcggcc gtgagggccg ccagccagct
     1861 aaccatctcg aaggtgccgg tgagcggctt cgcgaacgcg cgtaggaaag cgtcgagcac
     1921 cgtcacgaac atcatgcaaa cgagggccgc accggctact acggccatgc ccttgttgat
     1981 catctcgttg atcttgtcga ggtagtgcat caggggccgt cctccggacg cggtggtgga
     2041 tgtgggggcc ggccgggcga cgcgcccggc cggccccccc cgagagtcag gcggaaagcc
     2101 tacttggtgg cgttggcgtc acgcagctcg taaagcttgt cgaggaagcc ctggccgtcg
     2161 agacctttgg aggccatgtc ggcgatccaa gcgtcgacca tcggctggag cttcgtgtcc
     2221 cactcggcct tctggccttc gtcgagttcg acgatcttga ggccttgggt ttcctcagcc
     2281 cacttgaggg cgcccccgac gttgtcgttg tcgtggtagt tgccggtcca cagggccatc
     2341 tcacgaccga gatcgtcgat gactttctgg actggagccg gaagcgcgtc gaaggcgtcc
     2401 tgcttcatca cggccgcgaa cgtcacgacc acagtggggt agttggtgac gtacttgagt
     2461 tgctccgcga gtttgaagtc ttggagcact tcgcgcgagg tcatcgtgcc ttcgatcagg
     2521 ccggtctgca ccgcttcagg cacctcgttc atcggcatgc ctatgggcga tgcgccgagg
     2581 gcttcaagca ccggcacgcc cgtgccggcg gcacgcagct tcatgccctt gaggtcagcg
     2641 gcgttggcca caggcttctt ggtctggagg tagccgggct cggtggtgaa catcgtgatc
     2701 actttgaagc cgtcatactc ggccggcttg agctcggtgt agaggtccca cagggtcagg
     2761 ctgcccgtgg tggagtcggg gaagccgacg ggaagagaga cgcccgacgt cagcgggaag
     2821 cgtcctgggt cgtatgatgg gcagctcagc ccgatgtcgg ctacgccctg cagcacgccg
     2881 tcgtacatgt cgttggggcc gagcagggtg ccgccgggga aggtctcgat ggtcacgacc
     2941 ccgttggtcc gctcattgac caggcgcgcc cactcgtcca tctgcttgcc ggggaaggtg
     3001 ccggagggcg cgagaaggcg taggtcagct tgagggggtc caggccggcg aactcatcgg
     3061 cgggagccat agtcgtgtcg gtgccgtcgg tggacatgcc ggttgtcgtc gtgtcggcgg
     3121 ccgccgtcgt cgtggtcgtg ccgcccccgc agcctaccgc cacc
//
LOCUS       DASRMJ010000258         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_23612, whole genome shotgun sequence.
ACCESSION   DASRMJ010000258 DASRMJ010000000
VERSION     DASRMJ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23612"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /gene="cooS"
                     /locus_tag="VFD74_07090"
     CDS             <1..1455
                     /gene="cooS"
                     /locus_tag="VFD74_07090"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HZK49346.1"
                     /translation="AWLATTVTTGRMELFAKLGILPMGIDSTIAEVMHATTYGVDADP
                     VNILLTGLKCSLSDYAGMHLGTDLSDALFGTPTPRVSRANLGTLRVDSINVAVHGHNP
                     VLSDIICEVAVEMDEEAKAAGAPGGFNLVGVCCTGMEVQMRHGIPWATSSLSQELVIT
                     TGALEAMVVDYQCIQPAIADVAACYHTKVITTMSMAKMPGAIHVEFTEEHAKENAREI
                     LRLAIEAYPQRDSKRVDIPDITSTAFAGFSTETIVGALAKVDPDDPLKPLVDNIANGN
                     ILGVCLFAGCNDVKVTQDENFIVLAKALAKENVLLLATGCASGAFGRHGIFTQEAVAE
                     YAGDGLKAVLTAVGEANGLGGPLPLALHMGSCVDNTRALDVAVAVANYLGVDLDKLPV
                     VATAPEEAMEKAVSIGTWAVTMGLPTHVGVVPPVLGSAAVAGLLTAGIKDVTGGWFIV
                     EPDPEEAARKLIAAIVERRKGLNLPVPASAAALA"
     gene            1468..2049
                     /locus_tag="VFD74_07095"
     CDS             1468..2049
                     /locus_tag="VFD74_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZK49347.1"
                     /translation="MTGTSTAAGAARSAAPQEIYVVPARCTGCRSCEMACAVEHSQSK
                     SLFGAITETPPPRRRIYVEAVDTVAVPVACRHCDEAFCMNACITGAIRRDARGFVVLD
                     ESRCIGCNSCIMLCPFGVIQKDEARHVAAKCDLCPDLPGPACVAACPTDALRFEAAGD
                     FSRVRRRVAATVGAEAAGAAAGDCSGGGAAGAA"
     gene            2067..3422
                     /locus_tag="VFD74_07100"
     CDS             2067..3422
                     /locus_tag="VFD74_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK49348.1"
                     /translation="MARQYVIVGASAAGIGAAARLRELDPEAVITVLGEESHYAYYRP
                     LLPYLIDGRKTLHDISFWPEQWYAEQRIRLILGARGHRVEPDRKVVELDDGRRFDYDA
                     LLLAVGASSRVPDIPGVEAPGAYPLRTVADAEGIAARARAALGRAPSGGAEGAHAVLL
                     GAGLVSLKAGEPLMNLGVRCTFVVASGHPLSRVVDARVGEMVAERLRARGARLLFGRE
                     VSAITHAADGSVAGVTLDTGETLVCDLVIVGKGVAPNLDTAQRSGLACHRGVRAGSYL
                     ETTAPDVYAAGDCVETKDVVTGGQQVAALWTNAVSMAHIAAYNMVRGPEPARMRRFPG
                     VIGTMNSGVIAGIPIVALGLTDPVGDQYETVTAVERVAGSDDAGSTQGLRKLVFEADR
                     LVGAIFAGRVDRAGVYRSFIETGLPLGPTLRAAAEAGRLTYADVQMRGQKPPYVNYLQ
                     A"
     gene            complement(3469..4146)
                     /locus_tag="VFD74_07105"
     CDS             complement(3469..4146)
                     /locus_tag="VFD74_07105"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZK49349.1"
                     /translation="MTAFPHSEALDGRTASLLSRIAGEDAFRDFYLAGETALALQLGH
                     RMSLDLDLFSERPWSAGGVLDAARSLGPTTVDLESAGTLVGSIGGVRVSSFHYPYPLL
                     EPPLRFPGVALGAPPSIPLAGLLDIACMKLVAVSQRGSRKDFIDLYFLDRAGIDVRPA
                     VQALPRKILGVDHNLVHLIRSLAYFAGAESEPEPRMLVPFEWRQARDYALAESQTLLQ
                     EVLGPSA"
     gene            complement(4143..4319)
                     /locus_tag="VFD74_07110"
     CDS             complement(4143..4319)
                     /locus_tag="VFD74_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49350.1"
                     /translation="MFLIVRVLTAGRPDHVAWMFRRYPRSLIESTVSGDRRLSERVAG
                     AWANLLGLQPPVSR"
     gene            complement(4392..>4688)
                     /locus_tag="VFD74_07115"
     CDS             complement(4392..>4688)
                     /locus_tag="VFD74_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49351.1"
                     /translation="GLFMIGIAPVLYQYGAEITHPAPEGTSNGMFVLAMQVSVVFIYG
                     MGWLDGRLGSFTPSLMVLIAMLAATCLLFVGLKDVPARDTEVPVVVADAPASAG"
BASE COUNT          778 a         1576 c         1598 g          736 t
ORIGIN      
        1 gcctggctcg cgaccaccgt cacaactggg cgcatggagc tcttcgccaa gctgggcatc
       61 ctccccatgg gcatcgactc gaccatcgcc gaggtgatgc acgcgaccac ctacggtgtc
      121 gacgccgatc ccgtgaacat ccttcttacg ggactcaaat gctcactcag cgactacgcg
      181 ggcatgcatc tgggcaccga cctctccgac gccctcttcg gcaccccgac tccgcgcgtc
      241 agcagggcca acctggggac gctccgcgtg gactccatca acgtggccgt tcacggtcac
      301 aacccggtgc tctcggacat catctgcgaa gtggccgtgg agatggacga ggaggccaaa
      361 gccgcgggcg cgcctggcgg cttcaacttg gtgggcgtct gctgcacggg tatggaggtg
      421 cagatgcgcc acggcatccc ctgggccacg agttccctct cgcaggagtt ggtcatcact
      481 acgggcgccc tcgaggccat ggtggtcgac taccagtgta tccagcccgc gatcgccgat
      541 gtggccgcct gctaccacac caaggtcatc accacgatga gcatggccaa gatgccgggc
      601 gcgatccacg tcgagttcac cgaggagcac gccaaggaga acgcccgcga gatcctgcgg
      661 ctggccatcg aggcctaccc gcagcgcgat tcgaagcgcg tggacatccc cgacatcact
      721 tccaccgcct tcgccgggtt cagcaccgag accatcgtcg gagccctggc caaggtggac
      781 ccggacgacc cgctcaagcc tctcgtggac aacatcgcca acggcaatat cctgggcgtc
      841 tgcctgttcg ccggctgcaa cgacgtgaag gtcacgcagg acgagaactt catcgtgctg
      901 gcaaaagcgt tggccaagga gaatgtgttg ttgcttgcca ccgggtgcgc gtcgggtgcc
      961 ttcgggcgtc acggcatctt cactcaggaa gctgtggctg agtacgcggg cgacggcctg
     1021 aaggccgtgc tcactgctgt gggcgaggcc aacgggctgg gtggaccgct gccactggcg
     1081 ctccacatgg gctcgtgtgt agacaacacc cgcgcgctcg acgtggcggt ggccgtggcc
     1141 aactacctcg gcgtcgatct cgacaagctg ccggtggtag cgacggcgcc cgaagaggct
     1201 atggagaaag ccgtgtccat cggcacctgg gctgtgacca tgggcctgcc cacgcacgtg
     1261 ggtgtggtgc ccccggtgtt gggatcggcg gcagtggccg ggttgctcac cgcaggcatc
     1321 aaggacgtca ccggcggctg gttcatcgtg gagcccgacc cggaggaggc ggccaggaag
     1381 ctcatcgcgg ccatcgtgga gcgacgtaag gggctcaacc tgcccgttcc cgcgagcgca
     1441 gccgcgctgg cgtagggggt ggccgagatg accggcacca gcacagcagc cggcgccgcg
     1501 cgcagcgcgg cgccccaaga gatctacgtc gttcccgccc gctgcacagg ctgccgcagc
     1561 tgtgagatgg cgtgcgcggt tgagcactct caatcgaaga gtctcttcgg agcgatcacc
     1621 gagacgccgc ctccgcgccg gcgcatatac gtggaagccg tggacacggt cgccgtcccc
     1681 gtggcctgcc gacactgcga tgaggcgttc tgcatgaacg cctgcatcac gggggccatc
     1741 cgccgcgatg cccggggctt cgtcgtgctc gatgaatcac gctgcatcgg ctgcaactcg
     1801 tgcatcatgc tctgcccctt cggggtgatc cagaaggacg aagcgcgcca tgtggcggcg
     1861 aagtgtgacc tttgtcccga cctgcctgga ccggcctgcg tcgccgcctg tcccaccgac
     1921 gcccttcgtt tcgaggcggc cggcgacttc tcgcgtgtgc gccgccgggt tgcggcaacg
     1981 gtgggggcgg aggccgcggg agctgccgcg ggtgactgca gcggaggcgg cgccgccggc
     2041 gccgcctgac cccggggagg cgagtcatgg cacgccagta cgtgatcgtg ggcgcttcgg
     2101 cggccggcat cggcgcggcc gcgcgtttac gcgaactcga ccccgaggcc gtcatcacgg
     2161 tgcttgggga ggagtcccac tatgcatact accgcccctt gctcccctac ctcatcgacg
     2221 gacgcaagac gctccacgac atttccttct ggcccgagca gtggtatgca gaacaacgca
     2281 tccgactcat tctcggggct cgcggtcacc gggtcgagcc ggaccgcaag gttgtggaac
     2341 tcgatgacgg tcgtcgcttt gactacgacg cactgctgct ggctgtaggc gcctccagcc
     2401 gcgttccgga catccccggt gtggaggcgc ccggcgccta tcccctgcgc acggtggccg
     2461 atgccgaggg catcgccgcc cgcgcgcgcg cagccctcgg tcgtgcgccc tccggcggag
     2521 ccgagggcgc acacgccgtt cttcttggcg ccgggctcgt gagcctcaag gccggcgagc
     2581 ccctgatgaa cctgggtgtg cgctgtacct tcgtggtggc ctcgggtcac ccactttcgc
     2641 gggttgtcga cgcacgggtg ggtgagatgg tggccgagcg cctgcgggcc cgcggcgccc
     2701 gcctcctttt cgggcgcgag gtgagcgcga taacgcatgc tgccgacggc tccgtcgccg
     2761 gagtgacgct cgacacgggc gagaccctcg tgtgcgacct ggtgatcgtg gggaaaggtg
     2821 tcgctcccaa tctggacacc gcgcagcgca gcggcctcgc ctgccatcgg ggcgtacgcg
     2881 ccggttcgta tctggagacc accgccccgg atgtgtacgc cgccggcgac tgcgtggaga
     2941 ccaaagacgt cgtcacggga ggtcagcagg tggcggcgct ctggaccaac gccgtgagca
     3001 tggcccacat cgccgcgtac aacatggtgc gcggcccgga gccggcccgg atgcggcggt
     3061 tcccgggagt catcggcacg atgaactcgg gcgtcatcgc gggaatcccc attgtagccc
     3121 tcggcctcac cgacccggtg ggcgaccagt acgagacggt gacggcggtc gaacgagtgg
     3181 ccggatccga cgatgccggc agcactcaag gcctgcgcaa gctggttttc gaagcagaca
     3241 gactggttgg agcgatcttc gccggacggg tagaccgggc gggcgtctac cgcagcttca
     3301 tcgagaccgg cctacccttg ggcccgacct tgcgggcagc cgccgaggct ggccgactca
     3361 cctatgccga tgtgcagatg agaggacaga agccgcccta cgtcaactac cttcaggcct
     3421 gagctcctgg ccggtcgtgg gagtgcggtt gagcggcttg gaccgagctc aggcgcttgg
     3481 accgagaacc tcttggagca gggtctgcga ctccgcgaga gcgtagtcgc gggcttggcg
     3541 ccactcgaag ggcacgagca tccgaggctc gggctcggac tccgcgccgg cgaagtaggc
     3601 gaggctgcgg atgagatgca ccaagttgtg gtccacgccg aggatctttc ggggcagggc
     3661 ctggaccgcc ggacgtacgt cgattcccgc gcggtcgagg aagtagagat cgatgaagtc
     3721 cttgcggctt ccccgctgac tcacggccac gagtttcatg cacgcgatat cgaggagccc
     3781 tgccaacggg atcgagggcg gcgcgcccag cgcgactccc gggaagcgca gcggtggttc
     3841 gagcagcgga tatgggtagt ggaaggagct cacccgaacg ccgccgatgc tccccaccag
     3901 ggtgcccgcg ctttccagat cgacggtggt aggcccgagg gatcgggcgg catcgagaac
     3961 gccgccggcc gaccaagggc gttccgagaa caagtcgagg tccagtgaca tgcggtggcc
     4021 caactgtagc gcgagggcag tctcaccggc gagatagaag tcgcggaagg cgtcctcccc
     4081 cgcgattcgg gacagcagtg acgctgtgcg cccgtcgagg gcttcgctgt gcggaaaggc
     4141 ggtcatcggc tcaccggagg ctgcagcccg aggaggttcg cccacgcgcc cgcgacgcgc
     4201 tcactcagcc tcctgtcgcc ggacaccgtg ctctcgatga gcgagcgcgg gtatcgccgg
     4261 aacatccacg ccacgtggtc cggcctgccc gctgtgagca cacggacgat caagaacacc
     4321 gcgtctcgct cctcgtcgat gtgggcgggg tcggtgtccc agagtagagg ggccaggtcc
     4381 ggccgcaacg cctaccccgc cgacgccggc gcgtccgcca ccacaaccgg cacctcagtg
     4441 tctcgcgcgg gtacgtcctt gaggcccacg aacagcaggc aggtcgccgc cagcatagcg
     4501 atcaacacca tcagcgacgg agtgaacgag cccagccgac cgtccagcca acccatcccg
     4561 tagatgaaga ccaccgacac ctgcatggcc agcacgaaca tcccgttcga cgtgccctcg
     4621 ggcgccggat gggtgatctc ggcgccgtac tggtagagca cgggagcgat tccgatcatg
     4681 aagagtcc
//
LOCUS       DASRMJ010000259         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_241265, whole genome shotgun sequence.
ACCESSION   DASRMJ010000259 DASRMJ010000000
VERSION     DASRMJ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241265"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFD74_07120"
     CDS             complement(<1..416)
                     /locus_tag="VFD74_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK49352.1"
                     /translation="MEQPSPKVVRKAAQGEESALDSLVRSYYRPIQGYLERIVGDVSD
                     AQDLTQEVFLRMARGLPRFEGKAKFTTWLFQIAKNLGIDHLRRREIQRDPLQRVSEEA
                     ASAPVPEHGFEEHALLWSCIEALDVDLRSAVVLRDV"
     gene            complement(521..2041)
                     /gene="gpmI"
                     /locus_tag="VFD74_07125"
     CDS             complement(521..2041)
                     /gene="gpmI"
                     /locus_tag="VFD74_07125"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZK49353.1"
                     /translation="MVLDGWGYAEPGPGNAPSLARTPVIDDLLARYPWVLLEAAGVAV
                     GLPEGVMGNSEVGHLTLGSGRIVYQDLTRINKAVEDGSFFENPVLASAFEDAAKSGAS
                     IHLMGLLSDGGVHSAIEHIHALVELARRKGDANVFLHMFLDGRDTSPHAGEGFLADLE
                     TFLCGAGVGVIATIVGRYYAMDRDSRWERTKIAYDALVHGIGARAADAGVGLAASYAS
                     EVTDEFVLPVVTDEDPASRVRDGDTVVFFNFRPDRTRQLTRAFIEPGFDEFDRGGRPP
                     AVDFIGMTEYESSFDIPVAFPDDPPVNTLAEVLSGAGLRQLHIAETEKYAHVTFFFNG
                     GREDLLPGELHLLVPSPKDVETYDQCPEMSARGVAELFVETFRREPLDFVVLNFANPD
                     MVGHTGDLAATIEAIEVVDECLGRVLAALAEKDADVLVTSDHGNSEYMLYPDGTPNTA
                     HTTNPVRLVYLREGCLLRSGAGLSDIAPTVLSLLGVSVPPEMTGRDLCGGRRVTAV"
BASE COUNT          381 a          767 c          737 g          366 t
ORIGIN      
        1 tacacgtcac ggagcacgac ggccgaccgg aggtccacgt ccaacgcttc gatacacgac
       61 caaaggagcg cgtgctcctc gaacccgtgc tccgggaccg gcgcggacgc ggcctcttcg
      121 gatacccgct gaagagggtc ccgctggatc tcacgcctgc gcaggtggtc tatcccaagg
      181 ttcttcgcga tctggaagag ccaggtggtg aacttggcct taccctcgaa tctcgggagt
      241 ccccgcgcca tgcgcaggaa tacctcctgc gtcagatcct gcgcgtccga aacgtcgccg
      301 acgatccgtt ccaggtagcc ttggattggt cggtagtacg atcggacgag ggaatcgagc
      361 gccgattcct caccttgagc cgctttgcgg actactttgg gcgacggctg ctccactgtg
      421 tatctcttcc ccttctgaac cgtcagacga tcgtgatcgc ctgaagttag tgacgggccg
      481 ggccgcagtc gccccggcgc cgccggtgcg ggccggctct ctacaccgcc gtcacgcgcc
      541 gccctccgca gaggtctctg ccggtcatct ccggaggcac actcacgccg agcaggctca
      601 agacggtggg cgcgatgtca gacagtccgg cgccggagcg caagaggcat ccttccctaa
      661 ggtacaccaa acggacaggg ttggtggtgt gtgccgtgtt cggcgtgccg tcggggtaga
      721 gcatgtactc cgagttgccg tggtcggagg tgacgaggac gtcggcgtcc ttctcggcca
      781 acgcggccag gacacgcccg agacactcgt cgaccacctc tatcgcctcg atggtcgcgg
      841 cgaggtcgcc ggtgtgcccg accatgtcgg ggttcgcgaa gttcagcacg acgaagtcga
      901 gcggctcgcg tctgaatgtc tcaacgaaca gctcggcgac cccgcgggca ctcatctccg
      961 ggcactggtc gtaggtctcg acatccttgg gggacggcac caagaggtgc agctcgcccg
     1021 gcaacaggtc ctctctgccc ccgttgaaga agaacgtgac gtgcgcgtac ttctcggtct
     1081 cggcgatgtg gagttggcgc agacccgccc cactcagaac ctctgccagg gtgttcacgg
     1141 gggggtcgtc gggaaaggcc acggggatgt cgaaggatga ctcgtactcg gtcatgccga
     1201 tgaagtcgac ggcggggggc ctccctcccc ggtcgaactc atcgaatccc ggctcgatga
     1261 acgcgcgggt caactgcctc gtgcggtcgg gcctgaagtt gaagaagacg accgtatccc
     1321 catcacgcac tcgtgaggcc ggatcctcgt cggtgaccac gggcagcacg aactcgtcgg
     1381 tgacctcaga ggcgtaggac gccgccaggc ccacaccggc gtccgcagcg cgcgcgccga
     1441 tcccatggac cagggcgtcg taggcgatct tcgtgcgctc ccaacgtgaa tcgcggtcca
     1501 tggcgtagta ccgccccacg atcgtggcga tcaccccgac gccggcgcca cacaggaacg
     1561 tctcgagatc ggccaagaac ccttcgccgg cgtgcggaga cgtgtcccgc ccgtccagga
     1621 acatgtgcag gaagacgttg gcgtcgcctt tccgtcgcgc caactcgacg agcgcatgga
     1681 tgtgctcgat cgcagagtgc acgccaccat cagagagaag acccatgagg tggatcgagg
     1741 ctccactctt ggccgcatcc tcgaaggccg acgcaagcac ggggttctcg aagaaggaac
     1801 cgtcctccac ggccttgttg atgcgtgtga ggtcctgata gacgatgcgg cccgagccga
     1861 gagtgaggtg tcccacctcg gagttgccca tgactccctc gggtaggccc actgccacgc
     1921 cggcggcctc cagaagcacc cacgggtacc gcgcgagcag gtcgtcgatc accggcgtcc
     1981 gggctaggga cggcgcgttc cccggtcccg gttcggcata gccccagccg tcgaggacga
     2041 tgagggcgac cggccggccg gccggcgagg gctgcgtcga gcgagcggca tccgccttgc
     2101 ctttcggaac aaccgccggg ctgccggccg cccgagcgcc ctcggtcgcg ccacccgcca
     2161 ccgcgccgcc cgctgcccgt ccggctgcgg gcgggccggg gccgggccct tcgttctgag
     2221 cacgccgaat cacagagcgg cgtccaccaa c
//
LOCUS       DASRMJ010000260         5613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_381880, whole genome shotgun sequence.
ACCESSION   DASRMJ010000260 DASRMJ010000000
VERSION     DASRMJ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381880"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFD74_07130"
     CDS             complement(<1..195)
                     /locus_tag="VFD74_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49354.1"
                     /translation="MSTTALVIVGIVLLGGSALVMLWLLASHARSSAETAGSGVGETS
                     TPHPATEEPAAGMLGSPVVSV"
     gene            complement(251..766)
                     /locus_tag="VFD74_07135"
     CDS             complement(251..766)
                     /locus_tag="VFD74_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49355.1"
                     /translation="MARIDKDFLGLEVVSLEDASVVGEVDALLIDENATVAGLVIDLG
                     VYEAKVLAFSDILSIGEDAVMIETAASVKPISQNIALEEVAELDVHVTDTLVMTDRGD
                     LVGIVGDYYVDPATGAIKGLEILREDSEEETEEEEAYLVPSSQVIRIGAELVMLKAGF
                     EAAAVPSPDAL"
     gene            1100..2392
                     /gene="hemL"
                     /locus_tag="VFD74_07140"
     CDS             1100..2392
                     /gene="hemL"
                     /locus_tag="VFD74_07140"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZK49356.1"
                     /translation="MHVRYEKSSALYAEARRYLPGGVNSPVRAMRAVGREPVFMDRGD
                     GPRLWDVDGNEYLDYVGSWGPMILGHLHPAVVGALEAAFAKGTSFGTPSALEVEMARR
                     LVEAVPSVDMVRMTNSGTEATMSALRVARGHTGRDLILKFVGCYHGHGDSLLVSAGSG
                     VATFGLPDSPGVTQGTARDTLTVEFNDLAAAEEVFSRVGEQIAAVIVEPVAGNMGVVP
                     PAPGFLEGLRAVTSRYGAVLIFDEVMTGFRVAYSGAQERYGVTPDMTTLGKVIGGGLP
                     VGAFGGRADIMEKVSPAGSIYQAGTLSGNPLAMAAGIATLDVLKSGDAYQVLEARGLQ
                     LEVGLIAAAATAGVSARVQRVGAMLTVFFTERAVVDFPSAKTSDTGRFGVFFRGMLDR
                     GIYLAPSQFEAAFLSTAHTEEDIARTVGAASEVLGQFA"
     gene            2405..3049
                     /locus_tag="VFD74_07145"
     CDS             2405..3049
                     /locus_tag="VFD74_07145"
                     /inference="COORDINATES: protein motif:HMM:NF003989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HZK49357.1"
                     /translation="MADFVDKSKVPQATVPRLARYLRVLHRLERDGVPKVSSKELAER
                     LDANAAQIRKDFSYFGEFGVRGVGYEVKFLIGELSKCLGLDAEWRVVICGAGKLGQAL
                     ARYRVFGEEGFRLTGIFDSSRRVVGTDVDGLVIRSERELTTFTRENDVHIAIITVPAE
                     AAQAVADKAIEGGVKGILNFAPVRLALVEGVFIRQVDLSTELMVVSFYLSQLRR"
     gene            3108..4619
                     /locus_tag="VFD74_07150"
     CDS             3108..4619
                     /locus_tag="VFD74_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HZK49358.1"
                     /translation="MKDLRDFITLLREHGELAVIDHPVDPLLEITEIVDRVVKAGGPA
                     LLFRNVKGSSMPVLINQFGTHERTCLALRTASYDALGERVRLLFDLDMPEGVMEKVKA
                     LGRLRDLASFAPKRVKKAPCQDVVLRGDDVDLDLLPVLKCWPLDGGRFVTLPLVFTRD
                     PRTGVHNTGMYRLQVFDKKTTGMHWHLHKDAAEHFREASACGSDRLEVAVAIGTDPAV
                     TYAATAPLPGMVDEMMFAGFLRGEPVEMARCVTVDLEVPAHAEIVLEGFVDLSERRTE
                     GPFGDHTGYYSLADEYPVFHVTALTHRTDPIYATTIVGSPPMEDCFIAKATERLFLPL
                     LRLTLPEVVDMDLPMEGVFHNCAILSVKKAYPMHARKVMHAVWGTGQMQFTKFVIVVD
                     ADVDVHDYGQVAWRVFNNVDPKRDMVLAEGPLDALDHSSPTANWGGKMGIDATTKLAS
                     EGHPREWPPDIVMADDVVARVDAMWSQLGLGDIGSGTTGANARAPRRGAGTPR"
     gene            4654..5541
                     /locus_tag="VFD74_07155"
     CDS             4654..5541
                     /locus_tag="VFD74_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HZK49359.1"
                     /translation="MTAGRTRVDSWTRYPLLFVRLVKFEHSVFALPFAYSAAFLAEMR
                     VPGFWRMFWITVAMVSARSLAMALNRLLDASIDAKNPRTASRELPSGRLSRLEAWLFS
                     LASLAVLVVATFNLPVVTRYLWPIPVAAFVIYPLTKRWTWLCHLFLGATIGIGPIGAW
                     VAVTGEVTWTPFLLGGAVAAWIAGFDVIYAFMDVGFDRAQRFHSIPADFGASAGLWAT
                     RGLHVAAIALLAVIGREVGAGAVYYVGVAVCAALLCYENWIMRRADLEKVGVAFMTMN
                     GIISVVFVAFTTVAVLVAA"
BASE COUNT          861 a         1811 c         1930 g         1011 t
ORIGIN      
        1 gacggagacg acgggcgagc cgagcatccc cgccgcgggc tcttcggtcg cgggatgcgg
       61 cgtggacgtc tccccgacgc cggatcccgc cgtttcggcc gacgagcgag cgtgactcgc
      121 cagcagccaa agcattacca gcgcggaccc cccgaggaga acgatcccga ctatgacgag
      181 tgcggtggtg ctcacttcac gtgggccctg gtgtcggtgg ggccgctcgg acctctcggc
      241 gggtgcgcta tcagagggcg tccggggacg ggaccgccgc ggcttcgaac ccggccttca
      301 acatcaccag ttcggcgccg atcctgatca cctgggaaga cgggaccaga taggcttcct
      361 cttcctccgt ctcctcctcc gaatcctcgc gcaggatctc gagacccttg atggcgcccg
      421 tcgccgggtc gacatagtag tcgcccacga tgccgacgag gtcgccccgg tcggtcatga
      481 ccagtgtgtc tgtgacgtgc acatcgagct cggccacttc ctcgagggcg atgttctggg
      541 agatgggctt gacggatgcc gccgtctcga tcatgactgc atcctcaccg atgctgagga
      601 tgtcggagaa ggccagcacc ttcgcctcat acaccccgag gtcgatgacc agaccggcga
      661 ccgtcgcgtt ctcgtcgatg agcagagcgt cgacttcgcc gaccactgat gcgtcctcaa
      721 ggctgacgac ctcgaggcct aggaaatcct tgtctatgcg tgccatgtgg agccactcct
      781 cttcgtcccg cggggcgcat ccgcgcgcgg gcataacggt gtcgggatcc tctccgcatc
      841 ttaacagatg gccttcccgt gtggggacgt gccccggccg gcaccgatgc tcgtgtgttg
      901 ggctgaagag cgcgttcggc cgctactccg gaggctcgcc tccgcctccc gccccggagt
      961 ctcgtccagg ttggggcgtc actttgctta agttttcaca agacctgtgg tagattccat
     1021 tctgcggtgg tttcgacgag gggggggaac cgcgctcggc accgcgatgg ctcgtcaagt
     1081 ccatggcgtc gggggtacga tccacgtgag atacgaaaag agcagtgcac tgtacgcgga
     1141 ggcccgtcga tacttgcccg ggggggtgaa cagtccggtg cgggcgatga gggcggtcgg
     1201 gcgcgagccg gtgttcatgg accggggtga cgggcccagg ctgtgggacg tcgacgggaa
     1261 cgagtacctc gactacgtgg gctcgtgggg gccgatgatc cttggtcatc tccaccccgc
     1321 ggtcgtcggt gctctagaag cagcgttcgc gaaggggacg agtttcggca cgccgtcggc
     1381 tctcgaggtg gagatggcgc gtcgcttggt cgaggcggtc ccctccgtcg acatggtgcg
     1441 tatgaccaac tccggcaccg aagccactat gagcgccctc cgcgtggccc gcgggcacac
     1501 cgggcgcgac ctgatcctca agttcgtcgg ctgttaccac ggccacggtg actccctgct
     1561 cgtgagcgcg ggatcggggg tggccacgtt cggcctgccc gacagcccgg gagtcacgca
     1621 ggggaccgcg cgcgacacgc tcacggtcga gttcaacgac ctcgccgctg cggaagaggt
     1681 cttctctcgg gtcggtgagc agatcgctgc ggtgatcgtg gagcccgtag ccggcaacat
     1741 gggggtcgtc cctcccgcgc cgggattcct tgagggcctg cgggccgtca ccagccgcta
     1801 cggagcggtg ctcatcttcg acgaggtcat gaccggcttc cgggtcgcct acagtggagc
     1861 gcaagagcgc tacggggtca ccccggacat gaccaccctg ggcaaagtca tcggaggcgg
     1921 actgccggtg ggagccttcg gtgggcgcgc cgacatcatg gagaaggtct ctccggccgg
     1981 atcgatatat caggccggca cgttgtccgg caacccgctg gccatggccg cggggatcgc
     2041 gacgctcgac gtgctcaaga gcggcgacgc ctaccaggtc ctcgaggccc gcggcctgca
     2101 gttggaggtg ggcctcatag cggcggctgc gaccgcagga gtgtcggcgc gcgtgcagcg
     2161 cgtcggagcc atgctcacgg tgttcttcac cgagcgggcg gtcgtcgact tcccctcagc
     2221 caagaccagc gacaccggac gcttcggcgt cttctttcga gggatgctcg accgtgggat
     2281 atacctggcg cccagccagt tcgaggccgc cttcctgtct acggctcaca cggaggaaga
     2341 catcgcgcgc acagtcggag cggcctccga agtgctgggg cagttcgcat gacggggagg
     2401 catggtggct gatttcgtgg acaagtcgaa ggtgccgcag gcgactgtgc ctcgtctcgc
     2461 gcggtacctc cgcgtgctcc atcgtctaga gcgtgacggc gtgccgaagg tctcctcgaa
     2521 ggagctggcg gagaggctcg acgcgaacgc cgcacagatc aggaaagatt tcagctactt
     2581 cggcgagttc ggagtccggg gcgtcggcta cgaggtcaag ttcctcatag gggagctgtc
     2641 gaagtgcctg gggctggacg ccgagtggag ggtggtcata tgtggcgccg gcaagttggg
     2701 tcaagctctc gcccgttatc gcgtcttcgg cgaggagggg ttccgactga ccggcatctt
     2761 cgacagttcc cgccgcgtgg tgggcaccga cgtcgatggg ctggtcatcc ggtcggaacg
     2821 cgagctgact accttcaccc gtgagaacga cgtgcacatt gccatcatca cggtgccggc
     2881 cgaagcggcg caggctgtgg cggacaaggc gatcgagggg ggcgtcaagg gcatcctcaa
     2941 cttcgccccg gtgcgtctgg cgctcgtgga aggcgtgttc atccgccagg tcgacctgtc
     3001 cacagagctg atggtggtat cgttctacct gtcgcagctc cggcggtgat atcggcggcg
     3061 ccgagcgccg cgcccgcgac tcccggaccc aggagacgat accgacgatg aaggaccttc
     3121 gcgatttcat cacgcttctt cgcgagcacg gcgagcttgc ggtgatcgat catcccgtgg
     3181 atccgctgct cgagatcacg gagatagtcg acagggtggt gaaggccggt gggccggcac
     3241 tgctctttcg caacgtcaag ggctcgtcga tgccggtgtt gatcaaccag ttcggcacgc
     3301 atgagcgcac gtgtctggca ctgcgcacgg cctcctacga cgcgctgggt gagcgcgtcc
     3361 gcctcctctt cgacctggat atgcccgagg gcgtcatgga gaaggtcaag gccttgggtc
     3421 ggctgcgcga cctggcttcg ttcgcgccca agagagtcaa gaaggcgccg tgccaggacg
     3481 ttgtgctgcg cggcgatgac gtcgacctcg acctgctgcc tgtcctcaag tgctggcctc
     3541 tcgatggagg gcggttcgtc accctgccgc tggtgttcac ccgcgatccg cgcacgggag
     3601 tgcacaacac ggggatgtac cggctccaag tgttcgacaa gaagaccacc ggcatgcact
     3661 ggcacctgca caaggatgcc gccgagcact tccgggaggc gtctgcgtgc gggtcggatc
     3721 gtctggaagt ggccgtcgcc atcgggacgg acccggccgt gacctacgcg gccaccgcgc
     3781 ccttgcccgg catggtggac gagatgatgt tcgcgggctt cctgcgcggc gagcccgtgg
     3841 agatggcgcg ctgcgtcacg gtggatctcg aggtgccggc gcacgcggag atcgtgctgg
     3901 agggcttcgt cgacctctcc gagcgccgta ccgagggccc cttcggcgat cacaccggtt
     3961 actactctct cgccgacgag tacccggtgt tccacgtgac cgcgctcaca catcggaccg
     4021 acccgatcta cgccaccacc atcgtgggct cgccacccat ggaggactgt ttcatcgcga
     4081 aggccaccga gcggctcttc ctgccgttgc tgcgtctcac cttgcccgag gtggtcgaca
     4141 tggacctccc catggagggc gtctttcaca actgcgcgat cctttcggtg aagaaggcct
     4201 accccatgca cgcgcgcaag gtcatgcacg cggtgtgggg caccgggcag atgcagttca
     4261 ccaagttcgt catcgtggtc gacgccgacg tcgacgtgca cgactacggc caggtggcgt
     4321 ggcgggtgtt caacaacgtg gatccgaagc gcgacatggt gctcgcggag ggacctctcg
     4381 acgccctgga ccacagttca cccaccgcca actggggcgg caagatgggc atcgacgcca
     4441 ccacgaagct ggcgagcgaa ggacacccgc gtgagtggcc gccggacatc gtcatggccg
     4501 acgacgtggt ggcgcgggtg gatgccatgt ggtcgcaact cgggctcggc gacatcggat
     4561 cgggcaccac gggcgcgaac gctcgggcgc cgcggcgcgg agccggtacg ccgaggtgac
     4621 cacgaggcca ccgcgcccgg caggaccgtc gccgtgaccg cgggtcgcac acgcgtcgac
     4681 tcgtggaccc ggtacccact gctgttcgtg aggctcgtca agttcgagca ctcggtcttc
     4741 gcactgccct tcgcctactc ggcagccttc ctggccgaga tgagggtgcc cggcttctgg
     4801 cgcatgttct ggatcacagt ggcgatggta tccgcccgca gtctggccat ggcgctcaac
     4861 cgcctgctcg acgcctccat cgacgcgaag aatccccgca cggcttcacg cgagctcccc
     4921 tcaggcagac tctcgcggct ggaagcctgg ctgttctccc ttgcgtcctt ggcggtgctg
     4981 gtcgtggcca ccttcaacct gcccgtggtg acgcgctacc tgtggcccat cccggtggcg
     5041 gcctttgtga tctacccgct gaccaagcgc tggacctggc tgtgccacct gttcctcggc
     5101 gccaccatag gcatcggccc tatcggcgcg tgggtcgccg tgacgggcga ggtcacgtgg
     5161 acgcccttcc tgctgggcgg agcggtcgcc gcctggatcg ccgggttcga cgtcatctat
     5221 gcgttcatgg atgtggggtt cgaccgggct cagcgcttcc actcgatccc cgccgatttc
     5281 ggtgcgagcg ccggcttgtg ggctacgcgg ggtcttcatg tggcggcgat cgccctgctg
     5341 gcggtcatcg gccgcgaggt gggcgccggg gcggtgtact acgtgggggt ggcggtgtgc
     5401 gccgccctcc tgtgctacga aaactggatc atgcgccggg ccgatctcga gaaggtcggc
     5461 gtggccttca tgacgatgaa cggcatcatc agcgtggtct tcgtggcgtt cacgaccgtc
     5521 gccgtgctgg tggcggcgtg agcagctttg acccttgcct ggggcggcgg cgcggccgca
     5581 cgatgcgcgt ctgatgtcac cccgagtgga cgc
//
LOCUS       DASRMJ010000261         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_466698, whole genome shotgun sequence.
ACCESSION   DASRMJ010000261 DASRMJ010000000
VERSION     DASRMJ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466698"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFD74_07160"
     CDS             complement(<1..180)
                     /locus_tag="VFD74_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49360.1"
                     /translation="MLAALALAVGGLAVGLVGCGAEPAPVTTSGLSELLTEAEQGADE
                     TQDPAAFISLCTNCHD"
     gene            complement(270..977)
                     /locus_tag="VFD74_07165"
     CDS             complement(270..977)
                     /locus_tag="VFD74_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49361.1"
                     /translation="MMEEGKGYYVRFNWYHRLSHFVMMTTFIGCTLTGPPLKYSYDFW
                     AQTMMGFWGGVHNAGLIHRVMAGFMVADFLIHALYLAYWIVIKRANIFGPDSIMFRKK
                     DIADLVGMVKWFFDRGERPKFERFTFWEKFDWFAVFWGIAMIGTSGFALWFPTVTGQL
                     VSGIFLNVARIIHGDEAILAIGWILFVHLYNSHLRMNVFPMDPVIFTGKMPIEHLKHD
                     KAAWYERLVREGKIKPE"
     gene            complement(974..2002)
                     /locus_tag="VFD74_07170"
     CDS             complement(974..2002)
                     /locus_tag="VFD74_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZK49362.1"
                     /translation="MHRRRTGRGHPRGPVGLLSARRGWGLLLCAAVLTLGLVAPAAAR
                     DIEGCLICHGDPNFVDTTPAQGQDPSLYVDPEMFAQSVHAGNACSSCHQGFVLTIPQH
                     QIDASKSFRQVAIEACESCHPEAFMAYQTSTHAKVYYQGDVGPICISCHGSHAIRRVR
                     LGDESADFKMSMARDSCGRCHAEKFEQAREEFHFKALSLGYARAATCFDCHGFHDNLS
                     LKSGEAETLQACQKCHPGATTGFTYFQMHLDEGFVNAWWGVRVVYLFFSTLLIVVLVL
                     GIAYTTVHFQKETRIMLGVAGRGLGRLFGFIRALEDESVRGAVDDRIAADEESRSAGK
                     RADEEGGE"
     gene            complement(2061..>2253)
                     /locus_tag="VFD74_07175"
     CDS             complement(2061..>2253)
                     /locus_tag="VFD74_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49363.1"
                     /translation="HYIHDRPEEYANRLKAGTLHEVVRPAPTAGRRRFFRAVTTVVTL
                     FAFLAASYTLLLVLWPLVF"
BASE COUNT          437 a          727 c          700 g          389 t
ORIGIN      
        1 gtcgtgacag ttcgtgcaca gggagatgaa cgccgccggg tcttgggtct cgtcggcccc
       61 ctgctcggct tcggtcagga gctcggacaa tccggacgtc gtaaccggtg ccggttccgc
      121 accgcagccg accaggccga ccgccaggcc gcccacagcc aacgcaagcg ccgccagcat
      181 gaccgccaac ccgcctctcg caccggcatc gacgcgcggc cggcgcgcac tccggtccat
      241 cgaccgcccg gacgtgatcc gctcgcccat cattcgggtt tgatcttgcc ttcgcggaca
      301 agacgctcat accacgccgc cttgtcgtgt ttcagatgct cgataggcat cttcccggtg
      361 aagatgaccg ggtccatggg gaacacgttc atgcgcaggt gcgagttgta gagatgcacg
      421 aacaggatcc agccgatggc gaggatggcc tcgtccccgt ggatgatacg ggccacgttc
      481 aggaagatcc cggacacgag ctggccggtc acagtcggga accagagggc gaaaccggat
      541 gtgccgatca tggcgatgcc ccagaagacc gcgaaccagt cgaacttctc ccagaaagtg
      601 aagcgctcaa acttggggcg ttctccgcgg tcgaagaacc acttgaccat cccgacgagg
      661 tcggcgatgt ccttcttgcg gaacatgatc gagtcggggc cgaatatgtt ggcccgtttg
      721 atcacgatcc agtaggccag atatagggcg tggatcaaga agtcggccac catgaacccg
      781 gccatgaccc tgtggatgag gcccgcgttg tggaccccac cccagaaacc catcatggtc
      841 tgagcccaga agtcgtagct gtacttcagc ggcggccccg tcagggtgca cccgatgaag
      901 gtggtcatca tgacgaagtg gctcagtcgg tgataccagt tgaagcggac gtagtagccc
      961 ttgccttcct ccatcactca cccccctcct catcggcgcg tttgcccgcg gaccggctct
     1021 cttcgtcggc cgctatgcga tcgtccacgg cacctcttac ggattcgtct tcgagagcgc
     1081 ggatgaaccc gaagagtcgg cccagccctc gcccagcgac gccgagcatg atccgagtct
     1141 ccttctggaa gtgcacggtg gtgtacgcga tgccgagcac gagcaccacg atgagcaagg
     1201 tgctgaagaa gaggtacacc actcgcacac cccaccacgc gttgacgaag ccttcgtcga
     1261 ggtgcatctg gaagtaggtg aaccctgtgg tggcaccggg gtgacacttt tggcacgcct
     1321 gcagcgtctc tgcctccccg ctcttgaggg acaagttgtc atggaacccg tgacaatcga
     1381 agcaggtggc cgcccgcgca tacccaaggg agagggcttt gaagtggaac tcctcccgcg
     1441 cctgctcgaa cttctcggcg tggcaacggc cgcaggagtc acgggccatg ctcatcttga
     1501 agtcggcgct ctcatcgccc aggcgcacgc ggcggatagc gtgtgatccg tggcacgaga
     1561 tgcagatcgg gccgacgtca ccctggtagt agaccttcgc gtgcgtggag gtctggtacg
     1621 ccatgaaagc ctcagggtgg cagctctcgc aggcttcgat ggccacctgc ctgaacgact
     1681 tcgaggcgtc gatctggtgt tgggggatgg tgagcacgaa gccttggtga cagctggagc
     1741 acgcgttacc cgcgtgcacg gactgcgcga acatctcagg atccacatag agcgacgggt
     1801 cctgcccttg ggccggggtc gtgtcgacga agttggggtc accatggcag atcagacacc
     1861 cctcgatgtc gcgcgccgcc gccggagcga cgagaccgag tgtcaggacc gccgcgcaca
     1921 gcagcagccc ccacccgcgg cgcgccgata ggagcccgac cggcccccgg ggatgtccac
     1981 gtccggttct tcgtctatgc atcagatccc cttcgtattc tcagtgacga cactatccct
     2041 tcgcaatgac cgacttccga ctagaagacc agaggccaca gaacgagcag caacgtgtac
     2101 gaagccgcaa ggaacgcgaa cagggtgacg accgtggtga ccgcgcggaa gaaccgcctg
     2161 cgcccggccg ttggcgcagg acgcaccacc tcgtgcaacg tgccggcctt gagccgattg
     2221 gcgtactcct caggccggtc gtggatgtag tgc
//
LOCUS       DASRMJ010000262         8959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_348492, whole genome shotgun sequence.
ACCESSION   DASRMJ010000262 DASRMJ010000000
VERSION     DASRMJ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348492"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1278)
                     /locus_tag="VFD74_07180"
     CDS             complement(19..1278)
                     /locus_tag="VFD74_07180"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZK49364.1"
                     /translation="MGTDRSFASIEEALDEIKSGRMVVVVDDEDRENEGDLTMAAQFV
                     TPEAINFMAKFGRGLICLAITPGRADKLGLHPMARMNQSRFETAFTVSIEARDGVTTG
                     ISAADRAKTIQVAIDPRSGPDDLVQPGHVFPLRARPGGVLERTGQTEAGVDLARLAGL
                     NPAGVICEIMNEDGSMARVPDLIPYCGQHGLKMITVASLIEYRRRTERLVHRGAEAKL
                     PTHWGYFTAVGYESLLDGEQHLAVIKGDIAGKENVLVRVHSECLTGDVFGSLRCDCGE
                     QLAASMEVIEREGTGVLLYMAQEGRGIGLLNKLRAYKLQEEGRDTVEANVELGFAPDL
                     RDYGIGAQILSDLGLTTIRLMTNNPRKLVGVEGYGLTVVERVPVEVPPRDENLAYLQT
                     KVDKMGHILHHQGLHPEEIHVEGEGHE"
     gene            complement(1325..1990)
                     /locus_tag="VFD74_07185"
     CDS             complement(1325..1990)
                     /locus_tag="VFD74_07185"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZK49365.1"
                     /translation="MFTGLVEEVGRIARVEKRSQGAHIYVHAAVVTEGTRVGDSIAVD
                     GACLTVVHVEPKLFAVDCMAETLATTTIGARRAGDTVNLERAMALGERLGGHLVLGHV
                     DGVGEVRSVHRRGIALEVTVRFPADLAPYVAPKGSVAVDGISLTVTEVHDDEFGLGII
                     PHSLQHTTLGDVVAGRRVNLEADVLARYVRQALLAAGDAGRPSGGTGRLSMNGLRSAG
                     YLE"
     gene            complement(1990..3126)
                     /gene="ribD"
                     /locus_tag="VFD74_07190"
     CDS             complement(1990..3126)
                     /gene="ribD"
                     /locus_tag="VFD74_07190"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZK49366.1"
                     /translation="MALNDQDRHFLERAIALAERGRGLTSPNPLVGAVVVREGRVVGE
                     GFHAAAGQDHAEIVAMKSAVGTVTPGVLHGATMYVTLEPCCHQGRTPPCTQALIEAGV
                     TRVVVAADDPSTWVHGRGLEQLAAAGVEVDLADGDINYRARRQNDPFRKHVTRGLPFV
                     TYKYAMTLDGRVATESGDSRWISGEESRRRVHQMRAWSDAVLVGAGTLRHDDPRLTAR
                     DVAVHRQPLRVIVDPRLAIDHGSALVATVGEGPVLIVCADAVPEANRKKVASWGVEVV
                     AVDGGSTGRPAPEGVARLLADRQVQSVFMEGGPRLAAAWWDSGLIDRVLAFVAPVVAG
                     GAGSPGPLPSLGYPHMDAALRLRDVEITPSGADISISGYAAEPF"
     regulatory      complement(3176..3292)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3378..4082)
                     /gene="rpe"
                     /locus_tag="VFD74_07195"
     CDS             complement(3378..4082)
                     /gene="rpe"
                     /locus_tag="VFD74_07195"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZK49367.1"
                     /translation="MGIPADIFGKRLLAPSVLSADFSRLGEEVAAVLEAGVRLVHFDV
                     MDGRFVPNLTIGPAIVAAVAPQVHRGGAYVDVHLMVEHPDAFLDEFVAAGADALSVHV
                     ETTPNLHRTLARIRALGAAAGVVLNPATPVCVLEEAVLSADYVLVMSVDPGFGGQAFI
                     PESLDKLRRLRAVIPPRVALQVDGGVGRDNIARLVAAGADWLVAGSAVFGGGDATVQA
                     GVLQSLVDQAPGFGSE"
     gene            complement(4106..5047)
                     /locus_tag="VFD74_07200"
     CDS             complement(4106..5047)
                     /locus_tag="VFD74_07200"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZK49368.1"
                     /translation="MRFAFAGTPEFGAAVLKELMRGGRTPELVVSQPARPAGRGRRLT
                     ASPATVLARAEHLPCVETEDINSDDVLAALAGCRVDALVVAAFGQMLRTPLLTSFECV
                     NVHASLLPAYRGAAPIVRALMAGEEQTGVCIMRMTEGLDEGPVAARATIGVTPWDDRD
                     ALETTLALMGARAVAHVMDAMAGGYVQWRPQVGPTSYATKIGASDRVVDPNKTAAQVH
                     DHVRALSPVPGATVRIGDVPARLLRTWPGSGAGAAAGSVAGRPGEWACEGDRLFLGCG
                     EGWVEALTVQPSGKRIMTAAEFVRGYRSRLGRIARDS"
     gene            complement(5044..5556)
                     /gene="def"
                     /locus_tag="VFD74_07205"
     CDS             complement(5044..5556)
                     /gene="def"
                     /locus_tag="VFD74_07205"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZK49369.1"
                     /translation="MHGQHGAVDRIRTFGDPVLKQETRDVSTFGEDLVGLLDTMFDVM
                     DREQGIGLAAPQIGVQKRILVWRDPETEERHVLVNPRVVECSQETEAAEEGCLSIPGF
                     TMLVERPDCVLVEGRDALGRPLTLEASGLKARILQHEIDHLDGNLILDRTSVEERRRV
                     LKEIRQSMEQ"
     gene            complement(5570..7759)
                     /gene="priA"
                     /locus_tag="VFD74_07210"
     CDS             complement(5570..7759)
                     /gene="priA"
                     /locus_tag="VFD74_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZK49370.1"
                     /translation="MPGHTVQVTPLLGVRNLGREAFDYLVPEGFRDEIAVGSLVAIPF
                     GRRMIDGVVTAVGTAGDVDPARLRPIAAVGSRRVPAELAELAAVMSAHYLAPLGACFQ
                     AVVPLHRQASREGKSGRMVSWVAPAAVTVRDGSLTARQTEVLDAVPEAGVAVAELCAQ
                     LRVSRAVVAALVNKGALTTDLRRSKPTGSGTDTFPEARNASLELSVEQADVLRILRAD
                     LQRGGYREHLLWGVTGSGKTEVYLRILEEVLAAGGGGIVLVPEIALTVQLVERVHGRL
                     GDTVAILHSGLTPARRAHEYERIAQGVARIVVGARSAVFAPVSDLRVIILDEAHDSSY
                     KQDEEPRYDARWVARWLARRQGALLVEGTATPSVESLRPGTRPLEMRTRPTGAGAPTV
                     EIVDMRRQGAEGVLAPRSLAALRDTLVAGEQAVVLLNRRGYAAYLMCEECGDVVMCGD
                     CEISLTYHRGSRALVCHHCGRRVAVPAVCPSCSAAALRRGAPGTERVVDELLTMVPRE
                     ALFRLDSDVVTSGARVSDILARFRDAAPAVLVGTQMVAKGHDYPLVTLVLVADADTGL
                     YTPDFRAAERTFQLVTQVAGRAGRAEAPGKVLVQTWNPDVPCIRMAVAREEYRFYQEE
                     LASRERLGYPPYRELTRIVVSGPREDRVEAGGQHLAGKLRTVLEDVRGPARLPRIRGV
                     GRRQIVVADADARRVKIVVARSLERFRAPYGRRGLDIMVDVDPQWFN"
     gene            complement(7781..>8959)
                     /gene="metK"
                     /locus_tag="VFD74_07215"
     CDS             complement(7781..>8959)
                     /gene="metK"
                     /locus_tag="VFD74_07215"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZK49371.1"
                     /translation="SVSEGHPDKMADQISDAVLDAVLAADPYGRVACETLITNGLVVI
                     AGEITTHTYVDIPTIARRTIARIGYTRAKYGFDAETCGVVTSIHEQSPDIAQGVENAY
                     ERRTDPGDGDDLDLQGAGDQGMMFGYACRDTDVLMPLPIHLAHRLVHRASEVRKGDVM
                     PYLRPDAKSQVTVRYREGRPEFITAVVISSQHKDGIDIETLMRPDLIEHVIKPILPEG
                     LYDPEFKETKLYVNPTGKFVIGGPMGDTGLTGRKIIVDTYGGFGRHGGGAFSGKDPSK
                     VDRSGAYAARHVAKNIVAAGLADRCEVEIAYAIGVAHPVSVMVECFDTEVVPTSTIRE
                     LVLSHFDLRPAAIIRDLELRRPIYEKTAAYGHFGREDHDFTWERVDKAAALRSDAGLA
                     "
BASE COUNT         1477 a         3201 c         2759 g         1522 t
ORIGIN      
        1 cggcccgtct gcgcgttctc actcatgccc ttctccctcc acgtggatct cttccggatg
       61 aagtccttgg tggtgcaaga tatgacccat cttgtcgacc ttcgtctgca ggtaggccag
      121 gttctcgtcg cggggcggta cctccaccgg cacccgctct actacggtga ggccataccc
      181 ttctacgcct accagcttcc gcggattgtt ggtcataagc ctgatcgtgg tcaagccaag
      241 atcgctcagg atctgcgcac cgatgccgta gtcgcgcagg tcgggggcga agcccagttc
      301 cacgttggct tccaccgtgt cgcggccctc ttcctgcagc ttgtaggctc gaagcttgtt
      361 gagcaggccg atcccccgac cctcctgcgc catgtagagg aggacgcccg tgccctcacg
      421 ctcgatgacc tccatcgacg ccgccaactg ctcaccgcag tcgcaacgga gagagccgaa
      481 cacgtcgccg gtgaggcatt cggagtgcac tctcaccagg acattctcct tgccggcgat
      541 atcgcccttg atcaccgcga ggtgctgttc gccgtcgagg aggctctcgt atccgaccgc
      601 ggtgaagtag ccccaatgcg tggggagctt ggcttctgcg ccacggtgca ccagacgttc
      661 cgtacgtcgc cggtattcga tgagcgaggc caccgtgatc atcttcaagc cgtgctgccc
      721 gcaatacggt atcaagtcgg ggacgcgcgc catggagccg tcctcgttca tgatctcgca
      781 gatcacgccc gcgggattga gtccggcgag ccgggcgagg tccacgcccg cctcggtctg
      841 ccccgtgcgt tcgagcactc cccccggacg cgcccgcaga gggaacacgt gcccgggttg
      901 cactaggtcg tcggggccgc tcctcgggtc gatcgctacc tggatggtct tggctctgtc
      961 ggccgccgat atcccggttg tgacgccatc gcgcgcttcg atcgagacgg tgaaagcggt
     1021 ctcgaagcgc gattggttca tgcgggccat cgggtgaagt cccaacttgt cggcccggcc
     1081 cggcgtgatc gccaggcaga tgaggccgcg accgaacttg gccatgaagt tgatcgcctc
     1141 gggcgtcacg aattgagcgg ccatcgtgag gtcgccctcg ttctcgcggt cctcgtcatc
     1201 gaccaccacc accatgcgcc ccgacttgat ctcgtcgagg gcttcttcga tgctggcgaa
     1261 actcctatcc gtacccatgt cgctcccttc cagggtcggc gctaccgcga ccgaccgcgt
     1321 gatctcattc cagatagccg gcggagcgaa gtccgttcat gctcaaccga cctgtacctc
     1381 cgctcggccg ccctgcgtct cccgcggcga gcagagcctg ccgaacgtat cgcgcgagca
     1441 catccgcctc caggttcacg cgcctgcccg cgaccacgtc acccagggtc gtgtgctgca
     1501 gtgaatgcgg gatgatgccc aggccgaact cgtcgtcgtg tacctcggtc actgtgagcg
     1561 agatgccgtc gacggcgacc gaacctttag gggccacgta gggtgcgagg tccgccggga
     1621 acctcaccgt gacctccaag gcgatgccac gcctgtgcac cgatctgacc tcaccgaccc
     1681 cgtcgacgtg tccgagtacg aggtgaccac ccaagcgctc gcccagcgcc atcgcccgct
     1741 caaggttcac ggtgtcgcct gctctgcgag cgccgatcgt ggtagtcgcc agcgtctccg
     1801 ccatgcagtc gacagcgaag agcttcggct ccacgtgcac caccgtgagg caggctccgt
     1861 cgacggcgat ggaatcgccc actcgagtac cttccgtgac caccgccgcg tgcacgtaga
     1921 tgtgcgcacc ctgcgagcgc ttctcgacgc gggcgatcct tccgacttct tccacgagac
     1981 ctgtgaacat cagaacggct ccgccgcgta gccactgatg cttatgtccg ccccgctcgg
     2041 cgtgatctcc acgtcgcgca ggcggagcgc cgcgtccatg tgaggatacc cgagcgacgg
     2101 cagcgggccg ggcgacccgg cgccccccgc caccacaggg gccacgaacg cgaggacgcg
     2161 gtcgatcaac cccgaatccc accaagcggc ggcaaggcgg ggaccaccct ccatgaagac
     2221 gctctgcacc tgtcggtccg ccagcaaccg agccacgccc tcgggcgccg gcctacccgt
     2281 ggacccgccg tcgaccgcga ccacctccac accccacgag gcgaccttct tgcggttggc
     2341 ttccgggacg gcatccgcgc acacgatcag cacggggcct tctccgacgg tggcaacgag
     2401 cgcggaacca tggtctatgg caagacgcgg atccacgatg acacgcagcg gttgccggtg
     2461 caccgcgaca tcgcgcgccg tcaacctcgg atcgtcgtgc cgcagagtgc cggctcccac
     2521 cagaacggca tcagaccagg ctctcatctg gtgcacgcgt cgcctgctct cttcgccgga
     2581 gatccagcgc gaatcgccgc tctcggtggc caccctgccg tccaaggtca tagcgtactt
     2641 gtaggtgacg aaaggaaggc ctcgcgtgac gtgcttcctg aaggggtcgt tctgccgccg
     2701 ggcgcggtag ttgatgtcgc cgtccgccag atccacctct acgcccgcgg ccgccagttg
     2761 ttcaagtccc cgcccgtgga cccaggtcga ggggtcgtcc gccgccacca ccacccgggt
     2821 caccccggcc tcgatgaggg cctgcgtgca cggtggcgtt cgaccttgat ggcagcacgg
     2881 ctcaagagtc acatacatgg tggcgccatg aaggaccccg ggggtaacgg tgccgacggc
     2941 gctcttcatg gccacaatct ccgcgtgatc ctgccccgcc gcagcgtgga agccttctcc
     3001 cacgacccgg ccttcgcgaa caaccacagc gccgaccagc gggttggggg aagtcaggcc
     3061 gcggccacgc tccgccagag cgatggctcg ctccaagaag tgcctgtcct ggtcgttcag
     3121 ggccacaaaa aacccccaag ctgtcgtctc aggggtcggt ggcgcgcgta aacacgccgg
     3181 ccttctttca tccagacttt gactgtcggc ccaggagtca cacctggtct gccccgaagg
     3241 gctcgcgggc tctaccgccg gtagggaatc tcacccaacc ccgaagaccg ttctgatcct
     3301 gcaagcgcag gctgggtgaa tttaccacac ggccgacgac gcaacaacgg gcgcggaggc
     3361 gtctccggcc ctcgctccta ctcgctcccg aagccgggcg cctgatcgac gagagactgt
     3421 agaacacctg cttgcacggt ggcatcgccg ccaccgaaga cggccgaacc cgccaccagc
     3481 caatctgcac cggccgccac caagcgcgcg atgttgtcgc gtcccacgcc gccgtccacc
     3541 tgaagagcca cccttggagg gatgacagcc cgaaggcggc gcagtttgtc gagactctcc
     3601 gggatgaacg cctgcccacc gaacccgggg tccacgctca tgaccaatac gtaatccgcc
     3661 gacaggaccg cctcttccag cacgcagacg ggagtcgccg ggttcaagac cacccccgcc
     3721 gcggctccga gcgcccgaat gcgggcaagg gtgcgatgaa ggttgggtgt ggtctctacg
     3781 tggacgctca gggcgtccgc tcccgccgcc acgaactcat ccagaaacgc gtccggatgt
     3841 tcgaccataa ggtgcacgtc gacataggcc ccgccgcgat gaacctgggg cgccacggcc
     3901 gcgacaatcg ccgggccgat cgtcaggttg gggacgaagc gtccgtccat gacatcgaag
     3961 tggaccagtc tcactcccgc ctcgagcacg gccgcgacct cctcgcccaa ccgggagaag
     4021 tccgccgaga gcacagatgg cgcgaggagt ctcttgccga agatatccgc cgggatcccc
     4081 atgtctggtt ccttccgtcc gcgctctacg agtctcttgc gatccgaccc aacctggagc
     4141 ggtacccacg cacgaactcc gctgcggtca tgatcctctt tccgcttggc tgcacggtca
     4201 acgcctcgac ccagccctcc ccgcagccga ggaagagtcg atcgccctca caagcccact
     4261 cccccggcct gccggcgacg gatccggccg ccgctccggc acccgagccc ggccacgtgc
     4321 ggagcagtct cgccggcacg tctcctatgc ggaccgtcgc ccccggcacg gggctcaaag
     4381 cccgaacgtg gtcgtgcacc tgcgccgcgg tcttgttcgg gtcaacaaca cggtcggatg
     4441 ctccgatctt ggtggcgtac gaagtcggcc ccacctgcgg ccgccactgc acgtatccac
     4501 ccgccatcgc atccatgacg tgtgccacgg cacgcgcgcc catcagcgcc aaggtggtct
     4561 ccagtgcgtc gcggtcgtcc caaggtgtca caccgatggt agcgcgcgcg gctacgggcc
     4621 cttcgtccag cccttccgtc atgcgcatga tgcacacccc ggtctgctct tcccccgcca
     4681 tgagcgcgcg cacgatggga gcggcgcctc tgtaggcggg cagaagggac gcgtggacgt
     4741 tgacacactc gaacgacgtg aggagcggcg tgcgcaacat ctggccgaag gccgccacta
     4801 ccaaggcatc cacccgacac cccgccaagg ccgccagtac gtcgtcgctg tttatgtcct
     4861 cggtctcgac gcagggcagg tgctcggccc gcgccagcac agtcgccggc gacgcggtca
     4921 gcctgcgtcc gcgccccgcc ggcctggcgg gctgcgacac caccaactcc ggcgtccgcc
     4981 ctcctcgcat gagttccttg aggaccgctg ctccgaactc aggcgtgccg gcgaaggcga
     5041 atctcactgt tccatgctct gcctgatctc cttgagcacc cttctccgtt cctccacgga
     5101 agtgcggtcg aggatgaggt tcccatccaa gtgatcgatc tcgtgttgca ggatccgtgc
     5161 cttgagcccc gaggcctcca gcgtcagcgg cctgcccaaa gcatcgcggc cctccaccag
     5221 cacacagtcg gggcgctcca ccagcatggt gaagccgggg atggagaggc aaccctcctc
     5281 ggccgcttcg gtctcttgcg agcactccac gacgcgaggg ttcaccaaga cgtgtcgttc
     5341 ctccgtctcg ggatcgcgcc agacaagaat gcgcttctga acgcctatct ggggggccgc
     5401 caagccgatg ccttgctctc ggtccatcac gtcgaacatc gtgtccagga gccccacgag
     5461 gtcttcgccg aacgtggaga catcgcgagt ctcctgcttc agtaccggat caccgaaagt
     5521 cctgatgcga tccacagccc cgtgttgtcc gtgcatgtcg cctactccgt cagttgaacc
     5581 attgcgggtc gacatcgacc atgatgtcca acccccgcct gccgtacggt gccctgaatc
     5641 tctccagaga ccttgccaca actatcttga ccctgcgggc gtcggcgtcg gccaccacga
     5701 tctgccttct cccgacccct cgtatgcggg gcaaccgcgc cggacctctc acgtcttcga
     5761 gaacggttcg cagttttcct gccaggtgct gtccgccggc ttccaccctg tcctcgcgtg
     5821 ggccgctgac cacgatcctg gtgagctcgc ggtagggcgg atagcccaac cgctcgcggg
     5881 atgctagttc ttcctggtag aagcgatact cctcgcgcgc gacagccatt ctaatgcagg
     5941 gtacgtccgg attccaggtc tgcacgagca ccttgcccgg cgcctccgcg cgtcccgctc
     6001 tgcccgcaac ctgggtgaca agttggaacg tgcgctcggc ggccctgaag tcaggcgtgt
     6061 acaggccggt gtcggcgtcg gcgaccaaca ccagggtgac gagggggtag tcgtggccct
     6121 tggcgaccat ctgagtgcct accaacaccg ccggcgcagc atctcggaag cgcgctagga
     6181 tgtcggagac ccgggcaccg ctcgtcacga catcagaatc gaggcggaag agcgcttctc
     6241 gcggcaccat tgtgagcaat tcgtccacta cacgttcggt acccggggct ccccgccgga
     6301 gcgcggccgc agagcaactc ggacacaccg ccggaaccgc gacgcgccgg ccgcaatgat
     6361 ggcacacgag tgctctcgac cctcgatgat aggtgagtga gatctcacaa tcgccgcaca
     6421 tgacgacgtc cccgcactcc tcgcacatga gatatgcggc gtagccgcgg cggttgagta
     6481 gcacaacagc ctgctccccg gccaccagcg tgtctcgcaa ggccgcgagg gaccggggcg
     6541 ccaacacccc ttctgctcct tgtctccgca tgtctacgat ctcgaccgtg ggggcacccg
     6601 cccccgtggg acgcgtccgc atctccaagg gccgcgtccc ggggcgcaat gattccaccg
     6661 atggcgtggc cgtgccttct accagcagcg cgccctgccg gcgggccagc cagcgggcca
     6721 cccaccgggc gtcgtagcga ggttcctcgt cctgcttgta ggagctgtcg tgggcttcgt
     6781 cgaggatgat cacgcgcagg tcggagaccg gggcgaagac ggccgatcgg gccccgacga
     6841 cgatcctggc gaccccttgg gcgatcctct cgtactcatg cgcacggcgg gccggcgtga
     6901 gtccgctatg gaggatcgcg accgtgtctc ccagccgccc gtgcacccgt tctacgagct
     6961 gcaccgtgag agcgatctct ggaacaagga cgatgccccc tccccccgcc gccagcacct
     7021 cctcgaggat gcgcaggtag acctcggtct tgccgctccc ggtgaccccc cacaagagat
     7081 gctcgcgata gccgccccgc tggaggtctg ccctgaggat gcgcaacacg tccgcctgct
     7141 caacggagag ctccagggac gcgttcctcg cctccgggaa ggtgtccgtg ccgctgccgg
     7201 taggcttgga gcggcgcaga tctgtggtga gggcaccctt gttgacgagc gccgccacca
     7261 ccgctcggct cacccgcaac tgcgcacaca actcggccac ggccaccccg gcctcgggga
     7321 cggcgtcgag gacttcggtc tgtcgcgcgg tcagggagcc gtcgcgtacg gtcacagcgg
     7381 ccggtgcgac ccaggacacc atgcggccgc tctttccttc cctcgaggct tggcggtgaa
     7441 gggggacgac ggcctggaag caggccccga gcggggccag gtagtgcgcg gacatcaccg
     7501 cagccagctc cgccagctcc gcgggcacac gtcgggagcc aactgcggcg atcggccgca
     7561 agcgggcggg gtcaacgtcg cccgcggtgc ccacagccgt gacgactccg tcgatcatgc
     7621 gacgtccgaa cgggatcgcc accagcgaac cgacggcgat ttcatcccgg aatccttcgg
     7681 ggaccaggta gtcgaacgcc tcccgcccga ggttcctcac tccgagcagc ggggtcacct
     7741 ggaccgtatg gcccggcatg gagcgcccgc ctatgaggtg ctaggcgagg ccggcgtcgc
     7801 tgcgaagggc ggcggctttg tccacgcgct cccaggtgaa gtcgtggtcc tcgcggccga
     7861 agtgcccgta ggcagcggtc ttctcgtaga tcggccggcg cagctccagg tcgcggatga
     7921 tcgcggcggg acgcaggtcg aagtgcgaca gcaccagctc tctgatcgtg gaggtcggca
     7981 ccacttcggt gtcgaagcac tcgaccatga cgctcaccgg gtgcgccacg ccgatggcgt
     8041 aggcgatctc cacctcgcac cggtcggcca gccccgcggc cacgatgttc ttagccacgt
     8101 gccgcgctgc atacgcaccc gaacggtcca ccttcgacgg gtctttgccg gagaaggcgc
     8161 cgcccccgtg acggccgaag ccgccgtagg tatccacgat gatcttgcgg ccggtaagac
     8221 cggtatctcc catgggtccg ccgatgacga acttcccggt ggggttgacg tagagcttgg
     8281 tctctttgaa ctccggatcg tacaatccct cgggcaggat cggtttgatg acgtgctcta
     8341 tcaggtcggg ccgcatcagc gtctcgatat cgatgccatc cttgtgctgg ctcgagatca
     8401 ctaccgcggt gatgaactca ggccggcctt cgcgataacg caccgtcacc tggctcttcg
     8461 cgtcggggcg cagatagggc atgacgtcac ccttgcgcac ctcgctcgcc cgatgcacaa
     8521 ggcggtgggc cagatgtatg ggcagcggca tcagcacgtc ggtgtcccgg caggcgtagc
     8581 cgaacatcat gccctgatcc ccggcaccct gaaggtcgag gtcgtcgcca tccccggggt
     8641 ccgtgcgccg ctcgtacgcg ttctccactc cctgggcgat gtccggcgac tgctcgtgga
     8701 tgctcgtgac cactccgcag gtctcggcgt cgaagccgta cttggccctg gtatagccga
     8761 tgcgggcgat agtccgtcgg gctatggtgg ggatgtccac gtaggtgtgt gtggttatct
     8821 ctcccgcgat gacgacgagt ccgttcgtga tcaaggtctc gcaggccacc cggccgtagg
     8881 gatcggcggc gaggaccgca tccaggaccg cgtcagagat ctgatcggcc atcttgtcgg
     8941 gatggccctc gctcaccga
//
LOCUS       DASRMJ010000263         4202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_25570, whole genome shotgun sequence.
ACCESSION   DASRMJ010000263 DASRMJ010000000
VERSION     DASRMJ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25570"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1749)
                     /gene="ettA"
                     /locus_tag="VFD74_07220"
     CDS             complement(73..1749)
                     /gene="ettA"
                     /locus_tag="VFD74_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZK49372.1"
                     /translation="MAVDEKKVIYSMVRVGRVHPPKNQVLRDISLGFFYGAKIGVLGL
                     NGSGKSTLLRIMAGVDQDYVGEIATAKGYSVGFLEQEPKLDESKTTIQVIHEAVQPIV
                     DLLARFDAVTAGFSEPDADFDALIEEQGRLQEQLERVDAWNLDSRLEYAMGALDCPAA
                     DTPVSVLSGGERRRVALTRLLLTEPDILLLDEPTNHLDTESVAWLERHLRDYKGTVIA
                     VTHDRYFLDNVAGWILELDRGYGIPWKGNYSSWLEQKEERLRMEEKTESKRQKTLQRE
                     LEWIHMSQKARQAKGQARVNAYETLLSQDSEKRREDAEIYIPPGPRLGDVVIDLEGVK
                     KGYGDRVLLDGIAASIPPGSIVGVIGPNGAGKTTLLRLITGQETADAGSVLVGDTVKL
                     AYADQSRTLDDSKSVYDEISGGKETLTLGKREVNARAYCASFNFLGSDQQKKVGVLSG
                     GERNRVHLAKTLTEGANVILLDEPTNDLDVNTLRALEEAIDSFPGCVVVVSHDRWFLD
                     RVATHILAFEGECRARLVLGNWSDYEADYKERFGEDPGAGRARRYRGLER"
     gene            1807..2721
                     /locus_tag="VFD74_07225"
     CDS             1807..2721
                     /locus_tag="VFD74_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK49373.1"
                     /translation="MIDPSARIASLVVPKHHRAAPWLPLAAGVLWGTSFPAMGLALVG
                     FPPLTVAFLRALVGFLSLGAWLWLRGGLTSRPSPQQWLRLVALSLMGAGLFWPVLIFS
                     IELSSPVNSAFLTGIYPALAAGLAPLVLGERTRRANLLGLALALGGAYLVISKGRPLA
                     LFSSSTLTGDSLALLAALLFAAYILLGQKWRQVIGVSGEELTFYTFALALPPLGVLAM
                     AGGPLLTTVDVVAVAAIVWLGLMASTAAFLALNWGIRSGAVARGSMHLMVIPLVAAAC
                     SWLIFGTTMTPAQWLGGALVVLGVGASR"
     gene            complement(2788..3444)
                     /gene="sigH"
                     /locus_tag="VFD74_07230"
     CDS             complement(2788..3444)
                     /gene="sigH"
                     /locus_tag="VFD74_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HZK49374.1"
                     /translation="MESAARIGFPLIDWNDDEQLVRLAKVGNADATKRLLTKYHYFVR
                     MKSYSYFIAGGDGDDLIQEGYIGLYKAIRDYREDRARSFRSFAELCVTRQIITAIKTA
                     SRQKHSPLNSYLSFSYSPAGSNGNDDRTLADIIPSGRTSDPVMQVISREELESLTDCL
                     QRLLSPLESRVLRHYLEGRSYEVIAEMIDHDTKTVDNALQRIKRKVILHLESRRVSGQ
                     "
     gene            complement(3513..4052)
                     /locus_tag="VFD74_07235"
     CDS             complement(3513..4052)
                     /locus_tag="VFD74_07235"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZK49375.1"
                     /translation="MTVFLIDGYNVMHELCRSQGLDSDGQLAAGQLEDERARLLDRVA
                     SYMGGTSDRAIVVFDSSNASLQKGQSATANVQVYFGSLDRSADAIIERVTYAISAAEN
                     VVVVTSDYGLQKTIFLANVTRCSSRQFVSELQRHTSEVANSEKCTRMMHRVEDRIDSD
                     SLERLKELARRLAEESGGG"
     gene            complement(4099..>4202)
                     /locus_tag="VFD74_07240"
     CDS             complement(4099..>4202)
                     /locus_tag="VFD74_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZK49376.1"
                     /translation="PQLGKVGSLNVSVSAGVLLFEALRQRRVGEGDA"
BASE COUNT          676 a         1391 c         1301 g          834 t
ORIGIN      
        1 gccggcgccc cactctggtg cccgcactcg agcgacggcg cggcctgccg gacgcagggc
       61 cggagggacg agtcatcgct ccagcccgcg ataccgtcgt gcacgaccgg cgcccggatc
      121 ctcgccgaaa cgctccttgt agtcggcttc gtagtcggac cagttgccca ggaccaagcg
      181 ggctctgcat tcgccctcga aggccaggat gtgggtggcc acgcgatcga ggaaccagcg
      241 atcgtggctc accacgacca cgcagccggg gaaagaatcg atcgcctcct ccaaagcccg
      301 caacgtgttc acgtcgaggt cgttggtagg ctcgtcgagg aggatcacgt tggcgccctc
      361 ggtcaaggtc ttcgccaggt gcactcgatt gcgctcgccg ccggagagca cacccacctt
      421 cttctgctga tcagagccca agaagttgaa actggcgcag tatgccctcg cgttcacctc
      481 gcgcttcccc agcgtgagcg tctccttgcc cccggagatc tcgtcgtaga ccgacttcga
      541 gtcgtccaac gtgcgcgact ggtcggcgta tgcgagcttg accgtgtccc ccacgagaac
      601 ggagccggcg tcggctgtct cctgcccggt gatcaaccgc agcaaggtgg tcttgccggc
      661 tccgttcgga ccgatcacgc cgacgatcga gccgggcggg atgctcgcgg ctatgccgtc
      721 gaggagcact cggtcgccgt agcccttctt gaccccttcg aggtcgatga ccacgtctcc
      781 gagacgcggt cccggtggga tgtagatctc ggcgtcttcc cgccgcttct ccgaatcctg
      841 gctcagcaag gtctcgtagg cgttcacccg cgcctggccc ttggcctgcc gagccttctg
      901 cgacatgtgg atccactcga gctcgcgctg gagcgtcttc tggcgcttcg actcggtctt
      961 ctcctccatc cgcaagcgct cctccttctg ctcgagccaa gaggagtagt tgcctttcca
     1021 cgggataccg tagccgcggt cgagctctag gatccatccg gccacgttgt cgaggaagta
     1081 gcgatcgtgg gtgacggcga tgaccgtgcc cttgtagtca cgcaggtggc gttccagcca
     1141 tgccaccgat tccgtgtcga gatggttggt gggctcgtcg agaagcagga tgtcgggctc
     1201 ggtcaaaagc agcctggtga gggccacccg ccgccgctca ccacccgaca gcacgctcac
     1261 cggagtgtcc gccgccgggc agtcgagcgc gcccatggca tactccagac gcgagtcgag
     1321 gttccaggcg tcaacgcgct caagttgttc ttggagccgc ccctgctcct cgatgagagc
     1381 gtcgaaatcg gcgtccggct cggagaagcc ggcagtgacc gcatcgaagc gcgccaggag
     1441 gtcgacgatg ggctggacgg cctcgtggat cacctggatc gttgtcttgg attcgtcgag
     1501 cttgggctcc tgctccagga accccacgga gtagcccttg gcggtggcga tctcgccaac
     1561 gtagtcctga tcgacgccgg ccataatgcg cagcagggta ctcttccccg agccgttcag
     1621 cccgagaact ccgatcttgg ccccgtagaa aaaaccgaga gagatgtcgc ggagcacctg
     1681 gttcttgggc gggtgcacgc ggcccacccg caccatagag taaatgacct tcttctcatc
     1741 aaccgccata tacagctccc ggaatcgtag aggccacgtt ctgcgagatg ctaggatact
     1801 cgactcatga tagatccatc cgcaagaatt gcctcgctcg tcgtgccgaa gcaccacaga
     1861 gcggccccgt ggctgccgct cgcggccggc gtcttgtggg gcacgtcgtt cccggcgatg
     1921 ggcctggcgc tggtcggatt cccgcccctg accgtcgcct tcctaagggc gctggtcggg
     1981 tttctcagcc tcggcgcgtg gctgtggctt cgcggcggct tgacgagcag gccgtcgccc
     2041 caacaatggc tgcggctcgt ggcgctctcc ctgatgggag ccggcctctt ctggccggtg
     2101 ttgatcttct cgatcgaact ctcgagtccg gtcaattcgg ccttcttgac gggtatctac
     2161 ccggccctgg cagccggatt ggcgcccctc gtcttggggg agcgcacgag gcgcgccaac
     2221 ctcctcggtc tcgctctggc cctgggcgga gcctacctag ttatcagcaa ggggcgcccg
     2281 ctcgctctct tttcgagctc gaccttgacc ggcgactctc tggccttgct ggccgcctta
     2341 ctcttcgccg cctacatctt gcttgggcag aagtggcggc aggtcatcgg cgtgtcgggt
     2401 gaggaactta ccttctacac cttcgccctt gctctccccc cgctcggagt gctggccatg
     2461 gccgggggac cgcttctcac cacggtcgac gtcgtcgccg tagccgccat cgtctggctg
     2521 gggctcatgg cgtccaccgc agccttcctc gcgctgaact ggggcatccg atcgggagcg
     2581 gtcgctcggg ggagtatgca cctcatggtg atccccttgg tggccgccgc gtgcagctgg
     2641 ctcatcttcg gaaccacgat gaccccggcg cagtggttgg gcggggccct ggtcgtgctc
     2701 ggagtgggcg cctcgaggta gcacgtaccg ccgaggggct gcgcgcctcg gtccgccgct
     2761 cccaacaccg cgcccgcaac cgctgctcta ctgtccggag accctacgac tctcgaggtg
     2821 caagatgacc ttgcgcttga tgcgctgcag agcgttgtcc acagtcttgg tgtcgtggtc
     2881 gatcatctcc gcgatgacct cgtacgaccg cccttcgagg tagtgacgca acaccctgct
     2941 ctcaagaggg ctgagcaacc tttggaggca gtccgttagg ctctccagtt cctcgcggct
     3001 gatcacctgc atgacggggt cactcgtgcg accggagggg atgatgtcgg cgagggtacg
     3061 gtcgtcgttg ccgttgcttc cggccgggct gtagctgaaa ctcagatagc tgttcagggg
     3121 cgaatgcttc tggcgcgagg cggtcttgat cgcggtgatg atctgccgcg tcacacagag
     3181 ttcggcgaaa ctgcggaacg aacgagccct gtcctcgcgg tagtcacgga tggctttgta
     3241 gaggccgatg tagccctctt ggatcaggtc gtcgccgtcg ccgccggcta tgaaatacga
     3301 gtatgacttc atgcggacga agtagtgata cttcgtgagg aggcgcttcg tggcatcggc
     3361 gttgccgacc ttcgcgagcc gcaccagttg ctcgtcgtcg ttccagtcga tcaatgggaa
     3421 cccgatccgt gccgcgctct ccatacatgc ctcccctcgt tcgcccgtag cggcgacccg
     3481 cgcccttccg cgtcctaggt aagaggccga cctcaccccc cgccggactc ttcagcaagc
     3541 cgccgagcca actccttgag cctctcaaga ctgtcggagt ctatccgatc ctccacacga
     3601 tgcatcattc tagtgcattt ctcggaattt gcaacctcac ttgtgtgacg ttgcagttcg
     3661 gagacgaatt ggcgcgagga acagcgggtg acgttggcta ggaagatggt cttctggagg
     3721 ccgtagtcgg aggtaacgac caccacattc tccgctgcac taatggcata agtgactctc
     3781 tcgatgatgg cgtcggccga ccggtcgagg gagccgaagt agacctgcac attcgccgtt
     3841 gcactctgcc ccttctgcaa cgaggcattg ctgctgtcga agacgacgat ggctcggtcc
     3901 gatgtgccgc ccatgtagct ggcgacacgg tcaagcaatc gggcacgctc gtcctcgagt
     3961 tggcccgcgg cgagctgacc gtcagagtcg agtccttgag agcgacatag ctcgtgcatg
     4021 acgttgtagc cgtcgatgag gaacaccgtc atggacgatg cgccccctca ccgtgacgac
     4081 ccccgacgga cctggatctc acgcgtcacc ctctcccacg cgccgctggc gaagagcctc
     4141 gaagagcagc actcccgccg acacgctcac gttgagggac cccactttgc cgagctgcgg
     4201 ta
//
LOCUS       DASRMJ010000264         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_423783, whole genome shotgun sequence.
ACCESSION   DASRMJ010000264 DASRMJ010000000
VERSION     DASRMJ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423783"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFD74_07245"
     CDS             complement(<1..344)
                     /locus_tag="VFD74_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006822416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HZK49377.1"
                     /translation="MGLLGWVLRRIHDPEDPRAVFEPTGSQRDAASTLFNLSDYRVID
                     ALDLPNGGRRVEVEATSPPGCPACGVVSVRVHSRRRQRVRDIPVAGSLEVIWCKRRWF
                     CDEWRCERGRFA"
     gene            707..>2036
                     /locus_tag="VFD74_07250"
     CDS             707..>2036
                     /locus_tag="VFD74_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HZK49378.1"
                     /translation="MNANAKEKNPGLAEDEHDYPRSAPSDPSAPHVSVATVTVDAATD
                     RGALNRIWESFGYDEINWTYTRTGKRLLKTFKDFSGRPYYVRTHYVFCSGSGFGIPHW
                     GNGNVYHEDEQGNPYFDFNIADQTYDAIVKAGHHVLVELAFTPRDLLPEEAANLVVGN
                     SPTVYSNYEAGAWAYPPRDYEKWRSLIMAHAQHCLERYGEEEVNSWLWELWNEPDIFY
                     WRGTPQQFYDLYTVTAEAVRSVLPRAKVGGPAVTGGGVEFIRGFLRHTSANKLPLDFV
                     SFHTKGSAFTPWRVYGPTGGPAPEKQNPSTNKMLFEIRRMLRVIGEFEEYHSLPAIVD
                     ECDAGVPAHLSVYDNANFQFQNTEYYPVFQVKLMKKILDLNELEDVQVEAATSWSFYF
                     EGERYFEGTRSFLTAGGIEKPLLNAYRALARLGERRVAATSDAEHPVSLLD"
BASE COUNT          412 a          678 c          612 g          334 t
ORIGIN      
        1 tccgcgaacc tgccccgctc gcaccgccac tcgtcgcaga accaacggcg cttgcaccag
       61 atgacctcca acgacccagc gaccgggatg tcgcgcaccc gttgacggcg cctggagtgg
      121 acccggacag agaccacccc gcaggccggg caccccggcg gggacgtggc ctcgacctct
      181 acccggcgcc cgccgttggg caggtccagg gcgtcgatca cgcggtagtc ggacaggttg
      241 aaaagcgtgc tcgcagcatc acgctgcgag cccgtaggct cgaacacggc tcgtgggtcc
      301 tctggatcgt ggatgcgtcg taacacccat cccagcagac ccacgagttc tctacgtcac
      361 acgggcgccc gcaccggatt caccaccaaa cgggaagagc ccgcttcatg tgtccactga
      421 tgccagcgca catcgcgctg atgtgacatc tgagacatca acaccacaaa tcgcacagat
      481 aaatccgccc aaacccttgt ggacatcaca atggacgccc ataatcgtca cattccgtag
      541 cacggcggcc aggccgtctc tttaccgttg cagcgctgtc gacaccctag gacaccctcg
      601 aatggagaag ttctaggtga accaggtcac aacagggatc ctttgcgaca cgcgtcggag
      661 cagaagccag aactgattac gccgaactgg aaggcagcgc ccgcttgtga acgctaacgc
      721 taaagagaag aaccccggcc tagccgagga tgagcacgat tacccccgct ccgctcccag
      781 tgatcccagt gccccgcacg tgtcggtggc gacggtcacg gtcgatgccg ccaccgaccg
      841 cggcgcgctg aaccggatct gggagagttt cggctacgac gagatcaact ggacgtacac
      901 gcggacgggc aaacgactgc tcaagacgtt caaggatttc tccgggcggc cttactacgt
      961 ccggacgcac tacgtcttct gttccggcag cggtttcggt atcccgcatt ggggcaatgg
     1021 gaacgtctac cacgaggacg agcagggcaa cccgtacttc gacttcaaca tcgccgacca
     1081 gacttacgac gccattgtca aggccggcca ccacgtcctg gtcgaactgg ccttcacgcc
     1141 gcgcgacctg ctgccggagg aagcggcgaa tctcgtggtg ggcaacagcc cgaccgtcta
     1201 cagcaactac gaggctggcg cctgggctta tccgccccgt gactacgaga agtggcgaag
     1261 cctgattatg gcccatgccc agcactgtct tgagcggtac ggcgaggagg aggttaactc
     1321 ctggctctgg gagctgtgga acgaacctga catcttctac tggcgcggga ccccgcagca
     1381 gttctacgat ctctacaccg ttaccgcaga ggccgtccgc agcgtgcttc caagagcgaa
     1441 ggtcggcggg ccggcggtca ccggcggtgg cgtggagttc atacgaggct ttctgcggca
     1501 cacgtctgcc aacaagctgc cactcgactt cgtctccttc cacacgaagg gctcggcgtt
     1561 cacgccgtgg cgggtgtacg ggccgaccgg cgggccagcg cccgagaagc agaacccctc
     1621 gaccaacaag atgctgttcg agatccgccg aatgctgcgg gtaatcggcg agttcgagga
     1681 gtaccactcg ctgccggcca tcgtcgacga gtgcgacgcc ggtgtccccg ctcacttgag
     1741 cgtgtacgac aatgcgaact tccaattcca gaacaccgag tactaccccg tgttccaggt
     1801 caagctgatg aagaagatcc tcgacctgaa cgaactcgag gacgtccagg tggaggcggc
     1861 cacctcgtgg agcttctact tcgagggtga gcgctacttc gagggcacgc ggtccttcct
     1921 gaccgcgggc gggatcgaga agccgttgct gaacgcctac cgggccctcg cccgtctcgg
     1981 ggagcgccgg gtcgcggcga cgtcggacgc cgagcacccg gtcagcctgc tcgacg
//
LOCUS       DASRMJ010000265         7535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_202504, whole genome shotgun sequence.
ACCESSION   DASRMJ010000265 DASRMJ010000000
VERSION     DASRMJ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202504"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VFD74_07255"
     CDS             complement(<1..787)
                     /locus_tag="VFD74_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49379.1"
                     /translation="MTPAPAIVLKELTRDYGTTHAVDSLTLSIPQGEVFGFLGPNGAG
                     KTTTLKMLAGLISPTSGQAIVAGERVLPGSVSLSVGRKVGFLAEEPRFYPWMSGREFL
                     VFIGRVFGLGPSAAGDRADGLLQAMGLEDRGGERIRGYSRGMRQRLGIAHALMGDPEV
                     LLLDEPASALDPIGRKEILELIGSLRGKATIVMSSHVLEDVQRVATWVGVISQGRLLA
                     ESPLKELLRRYARPAYTLEVSGADERRAVARALASEPWLVECVE"
     gene            complement(784..984)
                     /locus_tag="VFD74_07260"
     CDS             complement(784..984)
                     /locus_tag="VFD74_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HZK49380.1"
                     /translation="MDERLTALLAVIIPLAIIQLGLMIWAIIDLVRRERVRGGSKLIW
                     ALVIVFVSTIGSILYLVWGRQE"
     gene            1107..1766
                     /locus_tag="VFD74_07265"
     CDS             1107..1766
                     /locus_tag="VFD74_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZK49381.1"
                     /translation="MGAGTGRLRVKRPYSLGEEIANSVIHGVGAGLSAAALALLVAFA
                     TRMSDGWRLAGAIVFGVTLLLEFAASTLYHSFSQPRVKHVFKILDHAGIYLLIAGTYT
                     PFILVTLRDSGYWWMLAVIWAMAVTGIVAEAFWVYRPKWVSVAVYLMMGWLVVVAIRP
                     LAAHLAEPGVWLLAAGGLAYTFGTIFYVLKKVPYTHAVWHLFVLGGAVCHFLVVALYV
                     I"
     gene            1810..2376
                     /locus_tag="VFD74_07270"
     CDS             1810..2376
                     /locus_tag="VFD74_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HZK49382.1"
                     /translation="MERTITYFESRGTANTDTVFELVAERAALLGINTVVVPSTAGAT
                     GLRAVENLKGLGVVVVSHSTGYGGPGTQQMPAETRAQIEAKGGKVLTTTHAFGGVGRS
                     VRKKFNTWQVEEIIAQTLKRMGEGTKVAVEVSLMAADAGMIPAHEEIIACGGSGRGLD
                     TALVLKPAHAQTFFDLEILEVICKPRTP"
     gene            complement(2528..3787)
                     /locus_tag="VFD74_07275"
     CDS             complement(2528..3787)
                     /locus_tag="VFD74_07275"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZK49383.1"
                     /translation="MRGTAELIFTGDELLRGDIVNTNQAYLGERLLELGVFATHAVSV
                     TDDREAISRAIVSSLLRHPDILILSGGLGPTEDDLTREAVSDALNRPLVLHEELLELI
                     RERFRMLDIHMGESNRKQALIPTGAQAIPIVGTAPGFWIEDGETLIAALPGVPRELEQ
                     MWTGTLGPLVSHRLGQREPAGEPAVRRLRLHGIGESTLADVLSEIPWRGEGVEIGTRA
                     SLDGITLILRAQPDRNSRERLAEVEERVRDVLGAKIFGADDETLAGVLGSLLRDRGLT
                     LATAESCTGGLVAKRLTDQAGSSDFFLGGVVTYGDALKTQLLGVDPDLLREHGAVSEE
                     VALAMARGARERLGATCSIAVTGIAGPTGGTASKPVGLVYVATAIGDEVLARRFNMFR
                     SRDEIRERTAYAALDVLRRRLLDSLDR"
     gene            3904..4473
                     /locus_tag="VFD74_07280"
     CDS             3904..4473
                     /locus_tag="VFD74_07280"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZK49384.1"
                     /translation="MPFEQLKLFRKVVKLKDGTLVVLRPLTPDDGGRLAAMFATIPEN
                     DRRLLRHDVTDAALVSAWVESLDYENVFPLVAQVDDEIIADATLHLNPGTSLRHTAEV
                     RVSIGGKARGRGLGLLMLEELVAAAGKLGLEQLRAEVPVGSSIAQRAFEKVGFRQVAI
                     FKDFFKTADGEYHDVAVMFLVLRDRWEEF"
     gene            4552..5172
                     /gene="hisIE"
                     /locus_tag="VFD74_07285"
     CDS             4552..5172
                     /gene="hisIE"
                     /locus_tag="VFD74_07285"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HZK49385.1"
                     /translation="MEIPDIAFDAHGLAPCIVQEWSTGEVLMLAYMNRESVEKTLATG
                     TTWFWSRSRASLWNKGETSGHVQKVRELRFDCDADALLALVEQTGVACHTGTASCFDG
                     RLLSAPAQTGPLFATLTDLESTIVGRKAAPAESSYTARLFGGGIPAIGAKVEEEAAEV
                     VEAAASGDLPHTVYEAGDVLYHLLVLLAHVGIPLSDVMAELERRKG"
     gene            5280..5858
                     /locus_tag="VFD74_07290"
     CDS             5280..5858
                     /locus_tag="VFD74_07290"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZK49386.1"
                     /translation="MLVVDNYDSFTYNLVQYLGEISTGDITVHRNDKITVEQIRDRRP
                     THIVISPGPCTPDEAGISLDVVRRLAGEFPILGVCLGHQAIGQAFGARVTRGEAPVHG
                     KTSEVHHDGRTVFHGIPTPFLATRYHSLVVRDLPSCLELSAWTDDDVIMGVRHREFAV
                     EGVQFHPESILTEPGKDILRNFLEVLSPLRCV"
     gene            6025..6855
                     /locus_tag="VFD74_07295"
     CDS             6025..6855
                     /locus_tag="VFD74_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZK49387.1"
                     /translation="MIKSILVGVDASAPAAAAREHAVELARAYQARVVGVHVLDVRLL
                     EMPPYLEYSYEGIPLIPMPTEILQGFREKGERVLEEFRRSLDGRGVAVESRLEEGIPA
                     ETIAEMGRTHDLIILGKRGEHARWGHDLLGTTTESVVRRASTPVWLAEQEVRPVRAIL
                     VMFDGSPPAGGALRLAADIATTLGATLRVLTVGDSEIQAGSVQEGARSYLDAFDLTTD
                     YRLAAGEAVTVALEHLEAEPPDLVVMGKQGHSLIRRLILGSTSEQLMREIPGPVLLAS
                     "
     gene            complement(6935..7423)
                     /locus_tag="VFD74_07300"
     CDS             complement(6935..7423)
                     /locus_tag="VFD74_07300"
                     /inference="COORDINATES: protein motif:HMM:NF045311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK49388.1"
                     /translation="MSRDLRERRYAERVGEKLRRLRQDRNLSLQEVCDRSGGSFVVST
                     LSAYERGKRSISLERLLELADLYGLSPTTLLEMEDEEEFQGSLGGNRPLRIRLEHLDR
                     LNDAERRPLENYLQFLKNLRNDPARQLLTIRKDDLNYLSSLYGVRPHALQERLKSEGI
                     LE"
BASE COUNT         1241 a         2431 c         2560 g         1303 t
ORIGIN      
        1 cctccacgca ctcgacgagc cagggctccg acgccaacgc tcgcgccacg gcccggcgct
       61 catcggcgcc ggacacctcc agcgtgtatg ccggtcgcgc ataccgtcga agcagctcct
      121 tgagcgggct ctcagcgagg agacggcctt ggctgatcac gccgacccac gtggccaccc
      181 gctgaacgtc ctcaagaacg tgtgacgaca tcacgatggt ggccttgccg cgcagggacc
      241 cgatgagttc aaggatctcc ttgcggccga tggggtcgag cgcggaggcg ggctcgtcga
      301 gcaggagcac ctcggggtct cccatcaagg cgtgggcgat ccccaggcgc tggcgcatgc
      361 cgcgcgagta ccctctgatc ctttcgccgc cgcgatcctc gaggcccatg gcctgcagca
      421 ggccgtcggc ccggtcgccc gccgcagagg gaccgagacc gaacacacgc ccgatgaaca
      481 cgaggaactc gcgaccgctc atccaggggt agaagcgcgg ctcttccgcc aagaacccca
      541 ccttgcgacc caccgagaga ctcaccgacc caggcagcac gcgttctccg gcgacgatag
      601 cctgtcccga cgtgggggag atgagtccgg cgagcatctt gagggtggtc gtcttgcccg
      661 cgccgttcgg tccgaggaag ccgaacacct cgccctgggg gatactgagg gtcagcgagt
      721 cgacggcgtg cgtggtgccg tagtcgcggg taagctcctt gagaacgatg gccggagcgg
      781 gagtcactct tggcgtcccc aaaccaggta gaggatcgac ccgatggtgc tgacgaagac
      841 gatcacgagc gcccagatga gcttgctgcc ccctcgcacg cgttcacgtc gaacgaggtc
      901 gatgatggcc cagatcatga gcccgagctg gatgatcgcc aacgggatga tgaccgccag
      961 caacgccgtg agccgctcat ccattctccg acctcccgtt cgtatcgggc acgcactctg
     1021 ccgccggatg ctacgtccat gcacggcggc cttcggcaca ttatacttgg ctgcatcgtc
     1081 ttctattcgg tcgatcggga ggttttgtgg gcgctgggac cggcagactg cgcgtgaagc
     1141 gaccgtactc gctcggcgag gagatcgcga acagcgtgat ccacggggtg ggcgcggggc
     1201 tgagcgccgc cgcgctcgcg ctgctcgtgg cctttgcgac ccggatgtcg gatggctgga
     1261 ggctcgccgg cgccatcgtc ttcggcgtga cgctcctgct tgagttcgcc gcgtcgacgc
     1321 tctaccacag cttctcgcaa ccccgagtca agcacgtgtt caagatcctc gaccacgccg
     1381 gcatctacct gctgatcgca gggacataca cgcccttcat cctcgtcacc ctgcgtgaca
     1441 gcggctactg gtggatgttg gctgtgatct gggccatggc ggtgacaggc atcgtggcgg
     1501 aggcgttctg ggtgtaccgg cccaaatggg tctccgtcgc ggtctacctc atgatggggt
     1561 ggctcgtggt cgtggcgatc cgtccgctgg cggcccacct ggcggagccg ggcgtgtggt
     1621 tgttggcggc gggaggtctc gcctacacgt tcggcaccat cttctacgtc ctaaagaagg
     1681 taccctacac gcacgccgtg tggcatctct tcgtattggg gggcgccgtg tgccacttcc
     1741 tggtcgtggc gctgtacgtg atctgagaag ctgagacgat ctaggaatcg agaatcagag
     1801 ggagtcattt tggagcgcac catcacctac ttcgagagcc gaggcacggc aaacaccgac
     1861 acggtgttcg aactcgtggc cgagcgggcg gccctattgg gcatcaacac tgtcgtggtg
     1921 ccgtccacgg cgggcgccac cggactacgg gcggtggaga atctgaaggg gcttggcgtg
     1981 gtggtcgtga gccactccac gggctacggc ggtccgggca cccagcagat gccggccgag
     2041 accagagctc agatcgaggc gaaggggggc aaggtcctca ccacgacgca cgcgttcggg
     2101 ggcgtgggcc gctccgtccg caagaagttc aacacctggc aggtggagga gatcatcgcg
     2161 cagacgctca agcgcatggg tgaagggacc aaggtggcag tcgaggtctc gctcatggcg
     2221 gctgatgcgg ggatgatccc ggcgcacgag gagataatcg cctgcggagg cagtggtcgc
     2281 ggtctggaca cggcgttggt gctgaagccc gcccatgcgc agacgttctt cgacctggag
     2341 atcctcgagg tcatctgcaa gccgcgcacg ccgtaggagt cggaggcgcc gggaagcgcc
     2401 gcgaggcgct cacgggtgct caggggaccc gcggggcgcc ggtccccccg ccgcatatgg
     2461 cggaccacgg aacgccggtg ctccccgtgc gcacgcccgt cggccggcgc gcgccctcga
     2521 ccgcggtcta ccggtcgagt gagtcgagca gacgcctgcg aaggacgtcg agtgcggcgt
     2581 aggcggtgcg ctcgcgtatc tcatcacgcg agcggaacat gttgaaccgg cgcgccagga
     2641 cttcgtcgcc gatcgccgtg gccacgtaga cgaggcccac gggtttcgaa gcggtgccac
     2701 ctgtcgggcc ggcgatgccg gtcacggcga tggagcacgt ggcccccagc cgctcccgcg
     2761 ctcctctggc catcgctaga gccacttcct cgctcacggc tccgtgctcg cgcaggaggt
     2821 ctggatcgac tcccaacagt tgggtcttca gggcgtcgcc gtaggtcacg acgcccccga
     2881 ggaagaagtc gctgctcccc gcctggtcgg tgaggcgctt cgcgacgagt ccgcccgtgc
     2941 acgactccgc agtggcgagg gtcaatcccc ggtcgcgcag gagcgaaccc aggacgccgg
     3001 ccagcgtctc gtcgtcggcg ccgaatatct tggctcccag tacgtcccgg acccgttcct
     3061 ccacctcggc caggcgctcg cgcgagttgc ggtccggctg ggcccgtagg atcagggtga
     3121 tcccgtccag cgaggctcgg gtcccgatct cgacaccctc tccccgccac ggtatctcgc
     3181 tcaacacgtc ggcaagcgtc gattccccga tgccgtgaag acgcagcctg cggacggcgg
     3241 gctcgcccgc cggttcccgc tgccccaacc tgtgggagac gagggggccg agggtccccg
     3301 tccacatctg ctcgagttcc cgcggtacgc ccggcagcgc ggcgatgaga gtctccccat
     3361 cttcgatcca gaagcccggt gccgtcccca cgatcgggat ggcctgcgcc cccgtgggga
     3421 tcagggcctg cttgcgattg ctttcgccca tatggatgtc gagcatgcgg aaccgttcgc
     3481 ggatcagctc aagaagttcc tcgtgcagca cgagaggtcg gttgagggca tccgagacgg
     3541 cttctcgtgt gaggtcgtcc tctgtcggac cgagaccccc cgagaggatc aggatgtccg
     3601 ggtggcgcag cagcgaggag acgatggccc gcgagatcgc ttcgcgatca tcagtcacag
     3661 agacggcgtg tgtggcgaag acgcccagtt cgagcagtcg ctctcccagg taggcttggt
     3721 tggtgttgac gatgtctccg cgcaggagtt cgtcgccggt gaagatcagc tccgcggtgc
     3781 cgcgcatgtc accatcctgg tcttgagcgt tggtgccggg tggtctcttc cgccttccca
     3841 tccccatcgg ccatatgtat catactctcg tgccgaagcc agccgaccgc gcggaggatg
     3901 cacatgccat tcgaacagct gaagctattt cgcaaggtcg tcaagctcaa ggacggcacc
     3961 ctcgtggtgc tccggcccct cacgcctgac gacggaggcc gcctcgcggc gatgttcgcc
     4021 accatccccg agaacgaccg tcgtctgctg cgccatgatg tgacggacgc ggccctcgtg
     4081 tcggcgtggg tggagagcct ggactacgag aacgtgttcc ctctggtggc gcaggtcgac
     4141 gacgagatca tcgcagatgc cacccttcac ctgaatccgg gaaccagcct gcgccatacc
     4201 gccgaagtga gggtctctat tggagggaag gcccgcggcc gcggattggg tctcctgatg
     4261 cttgaggagt tggtcgcagc cgccggcaag ctaggactcg agcagcttcg cgccgaagtg
     4321 ccggtgggtt cgagtatcgc tcaacgcgcg ttcgagaagg tcggcttccg tcaagtcgcc
     4381 atcttcaagg acttcttcaa gaccgccgac ggcgagtatc acgacgtggc agtcatgttc
     4441 ttggtcttgc gcgaccggtg ggaggagttc tagacgacca gcggcaccgt cgcgccgagt
     4501 cctctctgcg accaacggtc gaggcctagc ccggtctcgc gaaaggctct tatggagatc
     4561 cccgacatcg ccttcgatgc gcacggcctc gcgccctgca tcgtgcagga gtggtccacc
     4621 ggcgaggtgc tcatgctcgc ctacatgaac cgggagtccg tcgagaagac gctcgcgacc
     4681 gggaccacgt ggttctggag ccgttcccgt gcgagcctct ggaacaaggg ggagacctca
     4741 ggtcacgtgc agaaggtgcg cgaacttcgt ttcgactgtg acgccgacgc gttgctcgcc
     4801 ttggtcgagc agacaggtgt cgcgtgccac accggcacag cgagctgctt cgacgggcgc
     4861 ctgctgtccg cacccgcgca gaccgggccg ctcttcgcga cgctcaccga cctcgaaagc
     4921 accatcgtcg ggcgcaaagc ggcacctgcg gagagttcgt acaccgcccg tctctttggg
     4981 ggcggcatcc ccgcgatcgg ggccaaggta gaggaggagg cggctgaggt cgtggaggcg
     5041 gctgcgtccg gagacctgcc gcacaccgtg tacgaggcgg gagacgtgct ctaccacctg
     5101 ctggtgctct tggcgcacgt gggcatcccg ctctccgacg tcatggccga gctcgaacgc
     5161 cgcaaggggt aggcgtcatc gagcgcggca cggtcgacgt gacggagccg ggccacaccg
     5221 cgagtccgca ggtccggcgg ccggacgcgc cacctgttgc gccgcacctc gaggcgcgca
     5281 tcctggtcgt cgacaactac gactcgttca cgtacaactt ggtgcagtac ctgggcgaga
     5341 tctccaccgg cgacatcacg gtccaccgca acgacaagat aacggtggag cagatccgcg
     5401 accgccggcc cacccacatc gtgatctcgc ccggaccgtg cactcccgac gaagccggca
     5461 ttagcctcga cgtcgtcagg aggcttgcgg gggagttccc gatcttggga gtgtgcctcg
     5521 ggcatcaggc gatcggtcag gcgttcgggg ctcgggtgac gcgaggcgaa gcgcccgttc
     5581 acggcaagac ctccgaggtg caccacgacg ggcgcaccgt gttccacggc ataccgactc
     5641 cgtttctggc gacgcgctac cactcgttgg tggtgcggga tctcccttcc tgtcttgagc
     5701 tgagcgcctg gaccgacgat gacgtgatca tgggcgtgcg ccaccgcgag ttcgccgtcg
     5761 agggcgtgca gttccacccg gagagcatcc tcacggagcc gggtaaggat atcttgcgta
     5821 acttccttga ggtgctctct ccgctgcggt gcgtgtgagg cagcgcgccg cgcctcccgc
     5881 cgtccgggtg acgcagggcg acgcgcctcg attggatccg actcggtgtg gggatgacag
     5941 gaccgagtgc agcagattca ccggctccgt ggttgcgact tcagtcccgc gggctccgct
     6001 tccgtctcca aggagggtgg caagatgatc aagtcgatcc tggtgggtgt ggacgcctcc
     6061 gcaccggccg ccgcggcgcg agagcacgcc gtggagttgg cccgggcgta tcaggcgcgg
     6121 gtggtggggg tgcacgtcct cgacgtgcga ctcttggaga tgccgccgta tctggagtac
     6181 tcctacgagg gcatcccgct gatccccatg cccaccgaga ttctccaggg gttccgcgag
     6241 aagggagagc gggtcctcga ggagttccgc cgcagtctcg acggtcgcgg agtcgccgtc
     6301 gagtcgaggc tggaggaagg cataccggcg gagacgatcg cggagatggg gcgcacccat
     6361 gatctcatca tcctggggaa gcggggtgag cacgcgcgat gggggcacga tctcctcggc
     6421 acgaccaccg agtcggtggt gaggcgcgct tcgactccgg tgtggctggc ggagcaggag
     6481 gtgcgtccgg tcagggccat cctggtgatg ttcgacggca gtccgcccgc gggtggggcg
     6541 ctccgtttgg ccgccgacat cgctaccacg ctgggagcga cgttgcgcgt gctgacggtc
     6601 ggtgactcgg agattcaggc cgggtcagtg caggagggcg cgcggtcgta cctcgacgcc
     6661 ttcgatctca ccaccgacta tcggctcgcg gcgggcgagg cggtcacggt cgccttggag
     6721 cacctggaag cggagcctcc cgacctggtg gtcatgggca aacagggcca ctcattgatc
     6781 cgcagactga tactcggcag cacctcagag cagttgatgc gcgaaatacc cggaccggtg
     6841 ctgctggctt cgtaacacag gaggtcgacg ctcgaggttc cgagggagac gcccaccggc
     6901 gagactcgag gggcgaccga cggtcgccca agggctactc gaggatgccc tccgacttga
     6961 gtcgctcctg cagtgcgtgc gggcgcacac cgtataggga ggagaggtag ttgaggtcgt
     7021 ccttacggat ggtaaggagt tggcgcgctg ggtcgttgcg cagattcttg aggaactgga
     7081 ggtaattctc gagcggccgt ctctcggcgt cgttcaaacg gtccaggtgc tccagtcgga
     7141 tgcgcagcgg ccggttgccg ccgagactac cttggaactc ctcctcgtct tccatctcga
     7201 gcaaggtggt gggggagagt ccgtagaggt cggcaagctc gagaagcctc tccaggctga
     7261 tgctccgctt gccacgctca taggcgctga gtgtggagac gacgaacgag cctccagatc
     7321 tatcgcaaac ctcctggagg ctcaagttcc gatcttgccg aagtcgccga agtttctcgc
     7381 cgacacgctc cgcgtagcgg cgctctcgga gatctctgct cacccgcgct tcttgttccc
     7441 catccatgga gcaaatctac caggattcga gccgcaagac cagagtcgga gtgcccttca
     7501 tgcgcgagac cccgggggcg caggtgtggt gcggc
//
LOCUS       DASRMJ010000266         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_242226, whole genome shotgun sequence.
ACCESSION   DASRMJ010000266 DASRMJ010000000
VERSION     DASRMJ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242226"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFD74_07305"
     CDS             <1..553
                     /locus_tag="VFD74_07305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK49389.1"
                     /translation="DTVALYSGTMGKKQGLELLAAAAERLQGLPGLVFVFCGNGPGRE
                     QLETSCRHLPNVRFLDLQPPEHLGALLDMADIHLLPQQAGAADLVMPSKLTGMLASGR
                     PVVATSAEGTEIARVVRERGVIVPPGDVDALVGAIRELAADDNMRARLGANARAYAER
                     HLSRDVVLARFEKELCELVDGTG"
     gene            complement(971..1789)
                     /locus_tag="VFD74_07310"
     CDS             complement(971..1789)
                     /locus_tag="VFD74_07310"
                     /inference="COORDINATES: protein motif:HMM:NF023701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HZK49390.1"
                     /translation="MSLDPVLLRSLLAIEPYLDQVVVAGGWVPYLYQQLADQGSSQRP
                     PRTQDIDLAVPRDLVLKEHSVDELLKAVGFKCKFCSLDTPPVTKYVAGDDDEVEIEFI
                     TDAPGSKEGVRPVQPGLTAQELRYVGLLLENTRLVRLLPPNERLTVRVPSPGAFILHK
                     VLVVRRRREPVKKEKDLFYIFFVVVSFPEWRDAMADELAGLAQGRPRWFRRALKDLTA
                     WFESPDAEGISALLNQRPATAYPALTDNQFRQYAYDAVAELVDMMQAIADRDRI"
     gene            complement(1789..2904)
                     /locus_tag="VFD74_07315"
     CDS             complement(1789..2904)
                     /locus_tag="VFD74_07315"
                     /inference="COORDINATES: protein motif:HMM:NF021469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HZK49391.1"
                     /translation="MGNTTNAGNTRRSEEASLARLEEVLAEVLPGSQLRVLERAHGEA
                     IRRSDLEVEFNGVRLLVDFLESTSRVRLADTAEILRRECEAYDCTPVLAATYLSPSKQ
                     ELLRGLRTAFLDLAGNAWIVAPGIHIDRRGFPNRDKEPRERRDIYSDKATLVLRVLLT
                     RQKALGVREIAAIVRESAGGVGLSPGYVSKVAAELERRGYAGRRGDKVLLRHGPELLA
                     DWVAAYRSRRKPKNRGYFLPSPEAEALLPSLAPAFNEADVKYVFTGHAGASLVARFAL
                     FDVVDVYVKDAESADGVLTRMGAREVERGANVMVGLPHYLNSGFFDYQIPKPGMRTAS
                     DIQLYLDLYDHPVRGREQAGHLYERQLARLVEREGSL"
     gene            3074..>3786
                     /locus_tag="VFD74_07320"
     CDS             3074..>3786
                     /locus_tag="VFD74_07320"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49392.1"
                     /translation="MEEGTYTHTLAQPVAGPQRARARRMLPLLDAGSLVLSFAGALFL
                     LQARGVMDPTHRISHEILVAVAGVALLVFLFRDEQYSSGLRMSRLSDTIAVLKNAALA
                     YGVTLVAAFLTKGFFTGFANYSRTVILGSLALTVVLLFAERVALHAYQSRLFSRGEAL
                     RRVAVAGEGQATDELINFIEKRPWLGIRCVGHISTSADNSFGANAGPRSLPHLGSADT
                     LTEVAEQERVDEVLLALDPS"
BASE COUNT          677 a         1171 c         1193 g          745 t
ORIGIN      
        1 tgacacggtt gcgctctact cggggactat gggcaagaaa caggggttgg aactcttggc
       61 cgccgccgcc gagcgcctgc agggcctccc ggggctggtg ttcgtgttct gtggcaacgg
      121 tcctggacga gagcagctgg agacctcgtg caggcacttg cccaacgtcc gtttcctcga
      181 cctccagcct ccggaacacc tcggggcgct cttggacatg gccgacatcc acctgctgcc
      241 tcagcaggcc ggtgccgccg atctggtcat gccctccaaa ctcaccggca tgctggcgag
      301 cggccgcccg gtggtggcga cgtctgccga gggtacagag atagcccgag tggttcgcga
      361 gcggggggtg atcgttccgc ccggcgacgt ggacgctctt gtaggggcta ttcgcgaact
      421 ggcggccgac gataacatga gagcccgtct aggggcgaac gcccgcgcct acgcggagag
      481 gcatctctca cgggatgtgg tgttggcgag gttcgagaaa gagctttgtg aactggttga
      541 cgggaccggc tgagcgagcc ctcggagctt gcggagcagg gcctgtacaa tgtaccgatg
      601 gggcgatttg gccggatgcg ggcgcgccac aatcaacggt ggtccgggct tcgccactag
      661 tccggaatgg tcaaactcca tgtctgcgcc aagagacctc caatggccca ggatgcgcgt
      721 tcgcggctgg cagtgaactc gggggaatag gctgcttggc ccgtgtccca tcgttctaca
      781 cctcctttct caggggagaa cgggacttgg attctaaacc cggggcggtt cagatattga
      841 cgaatcagtt cggctccgcg atggcgatgg cggttgcggg atacggctac tggagcccgg
      901 taggggggac cctctgactg ctcccagacc cagcgggcgc ttcacggtct tgaccgcggg
      961 gcggggcgta ctagatacgg tctcggtcag ctatcgcttg catcatgtcg accagttccg
     1021 ccactgcatc gtaggcgtac tgacggaact ggttatcggt tagagccggg taggctgttg
     1081 cgggccgttg gttcagaagc gctgatatcc cctctgcatc gggactttcg aaccaggcgg
     1141 tgaggtcttt cagcgccctg cggaaccagc gcggcctccc ttgagccaaa cctgccaact
     1201 cgtcagccat cgcgtctctc cactcaggga aggacaccac tacaaagaag atgtagaaga
     1261 ggtccttctc cttcttcacc ggctcgcgcc gtctcctgac cacaagcacc ttgtggagga
     1321 tgaacgcacc tggcgacgga accctcacgg tgagacgttc gttgggcggc aacagccgta
     1381 ccagccgcgt gttctccagc aggagcccga cgtaccgcag ctcctgcgcg gtcaaccccg
     1441 gctggacggg gcgaacgccc tccttggatc ccggagcgtc ggtgatgaac tcgatctcca
     1501 cttcgtcgtc atctccggcg acgtactttg tgacaggagg cgtatcgagt gaacagaact
     1561 tgcacttgaa gccaaccgct ttgaggagtt cgtctaccga atgctccttg agcaccaagt
     1621 cccgtggaac ggccaggtcg atgtcctggg ttcgcggcgg ccgctgtgat gaaccctggt
     1681 cggccaactg ctgatagagg tagggcaccc acccgccggc gaccacgacc tgatcgagat
     1741 acggctcaat ggccagaagg ctgcgcagga gcacaggatc gaggctcatc acaaggaacc
     1801 ctcgcgctcc acaagacggg caagctgtcg ctcatatagg tgccccgcct gctcccgccc
     1861 ccggacgggg tgatcgtata ggtcgaggta gagttgaatg tcggaggcag tcctcatccc
     1921 cggcttcgga atctgatagt cgaagaagcc cgagttcagg tagtggggca ggccaaccat
     1981 cacgtttgcc ccgcgttcga cttctcgtgc gcccattcgg gtgagaacgc cgtctgcgct
     2041 ttccgcatct ttaacataga cgtctactac gtcgaagaga gcgaagcggg ccaccaggct
     2101 ggctcccgcg tggccggtga agacgtactt gacatccgcc tcattgaaag ccggcgcgag
     2161 ggatgggagg agtgcctccg cctccggcga aggcagaaaa taaccccggt tctttggctt
     2221 ccggcgagaa cggtaggcgg ccacccagtc cgccagcagt tccgggccgt gacgcagcag
     2281 caccttgtcc ccccgccgac ccgcgtatcc ccggcgctcc agttcggccg cgactttgga
     2341 gacgtagccg gggctcaaac cgaccccgcc tgcggactca cggacgattg cggcgatctc
     2401 tcgaactccc agggccttct gtcgggtgag gagaacgcgc aataccagcg tggctttgtc
     2461 cgaatagatg tcccggcgct cgcgaggctc cttatcgcgg ttgggaaaac cccggcgatc
     2521 gatgtggatc ccaggcgcca cgatccaggc gttaccggcc aagtcgagga acgccgtgcg
     2581 cagtccccga aggagttctt gctttgacgg actcagatac gtcgccgcga ggacgggggt
     2641 gcagtcgtag gcctcacatt cgcggcgcag gatttcggca gtatcggcca agcgaaccct
     2701 gcttgttgac tcgaggaaat cgacgagtag gcgtacgccg ttgaactcca cttcgaggtc
     2761 gctacggcgg atggcttccc cgtgagcccg ctcgaggacg cgcagttgcg agcccgggag
     2821 cacttccgcg agaacttcct cgagtctggc gagggacgct tcctcgcttc gtctggtgtt
     2881 tcctgcattt gttgtgtttc ccatacggga aacagtgtac ttgtaggaaa catacaaatc
     2941 aagcgttcgt ggtctttggc cgatgcggaa tagccaagat cgccgcttct gtccgcccca
     3001 tatgctataa aggtatacga acgcccgcgg cgtactgtag gcttaagggc aaccggctag
     3061 agtagaggag accatggagg aagggaccta cactcatacg ctggctcaac cggtagcagg
     3121 gccgcagcgg gctcgtgcgc ggcgcatgct accgctgctg gatgccggca gcctcgtgct
     3181 ctcattcgcc ggtgccctct tcctgctcca ggcgcgggga gttatggacc caacgcaccg
     3241 tatctcccac gagattctgg tagcggtcgc cggggtcgcc ctgctcgtgt ttctcttccg
     3301 ggacgagcag tactcttccg gcctgcgcat gtcccgtctc tccgatacca ttgccgttct
     3361 gaaaaatgcg gctctcgcct acggtgtcac tctggtggca gcatttctca ccaagggctt
     3421 tttcacaggc tttgccaact actcccgcac agtgatcctg gggagccttg cccttacggt
     3481 agtgcttctc tttgccgagc gggtggccct tcacgcctat cagtcccgcc ttttttcccg
     3541 cggtgaggcc ctccgccggg tggcggtggc gggagaagga caggccaccg acgagcttat
     3601 taatttcata gagaagcgcc cgtggctggg tattcggtgt gtggggcaca tcagtacgtc
     3661 cgcagataac agcttcggcg ccaacgcggg tccgcgttcg cttccccacc tgggctccgc
     3721 cgacaccctt accgaggtcg cggagcagga gcgcgtggac gaggttctgc tggccctgga
     3781 tccctc
//
LOCUS       DASRMJ010000267         4998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_141071, whole genome shotgun sequence.
ACCESSION   DASRMJ010000267 DASRMJ010000000
VERSION     DASRMJ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141071"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1880)
                     /locus_tag="VFD74_07325"
     CDS             complement(<1..1880)
                     /locus_tag="VFD74_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK49393.1"
                     /translation="MVVGAGISGIQAALDLATDGFKVYLVEKAPTIGGKMSQLDKTFP
                     TNDCSMCIESPKFIECNRHPNIEIMTYTEVERVEGEAGDFNVTLIRKPRYVKEDVCTG
                     CNTCMEYCPVMVPDKYNQSLSDNKAIHIHFSQAVPLVTYVDEEHCLYLKDKKCNICVG
                     VCKNKAIDLHQKAEKVVVNVGAVVLSPGYEVFDPRLRGDYGYGQMQNVVTSLDYERLL
                     CSTGPFEGEALRPSDKKHPKKIAWIHCVGSRQVIPGGNTYCSAVCCSYTQKQVILSKD
                     HDADIEATVFHNDIRSYGKDFERFYQRAANLPGVRFIRSYVSVGREIPETNNVTIRYS
                     TTEDGVKEEEFDLVVLSVGMNPPSDAEDMATKFGVELDSYGFCKTDPFNPILTSRPGV
                     FVSGAFNGPVDIPESVVTASGAGSLSGELLAYRRGKLARDRIYPTERDVSEEETKVGV
                     FVCHCGANIGRVVDVPSVVDYSLGLENVVHAEEGLFICSTDAAQQISETIREKGLNRV
                     VVAACTPRTHEPLFRDTLREGGINQYFFDFANIREHCSWVHSREKEAATAKAKDIVRM
                     AVARAGCLEPLEEYDLPVNKALLVVGGGVAGMTSALSVANQGFEVYLVEKDHDLGGMA
                     RRL"
     gene            complement(1962..3137)
                     /locus_tag="VFD74_07330"
     CDS             complement(1962..3137)
                     /locus_tag="VFD74_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZK49394.1"
                     /translation="METENLSDYAEIRELVDENGGAAFDRCYQCGLCDVVCPWNLVRD
                     FSMRKIVREATFGMTEIEEEEIWRCTTCGRCPQRCPRDVKQIESGVALRRIATEFKVF
                     PTAVQPVRTARASLLSDGNPLGQKREDRGKWAQGLSVKTFSEGMEFLYFASCYLSYDP
                     RLTKVAKATATLLNKAGVEYGILGSKDSCCGESIRKTGDEDVFKRLARDNIKSFIDAG
                     VSKILVSSPHCYHTFKNEYPDFHAHFEVVHISELLLDLVKQGRLELSHEYAKRVTYHD
                     PCYLGRHNGIYEEPRSLLGSVPGLELVEMSDSRVDSLCCGGGGGRIWMETPKEERFSN
                     IRMAQAVEVGAEVLATSCPYCITMFEEARLSADQSSAPVVKDITEIVLESLQGTVDA"
     gene            complement(3172..3720)
                     /locus_tag="VFD74_07335"
     CDS             complement(3172..3720)
                     /locus_tag="VFD74_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK49395.1"
                     /translation="MTETAEKRVVKEIKVDLDKCIGCRACEVACSAFHARPRFSATNP
                     ARSRIRVIIDELSDLYVPIRAGDNTSAECTGRHIYAINGKEYSECSFCGVSCPSRDYF
                     YEPDSGLPLKCDMCEEDPTLKEPWCVQVCRCDCLTYTEREDEKPVEEEKRGEMEIGLE
                     SLAKKFGMDKILDTVARMSSRG"
     gene            complement(3732..>4998)
                     /locus_tag="VFD74_07340"
     CDS             complement(3732..>4998)
                     /locus_tag="VFD74_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZK49396.1"
                     /translation="LLAHEDDEEFHVNNFAWGNARDRIKGYWNHEREERWKEISDNAR
                     VRWTGCYNCPKDCHQAITYPGRMPYFQKCYSKLTYAMAAYEELDFNYDILGLTQEYGL
                     DGFSTPQVLAFAVELYDAGILTDEDVPGFPSDGAGRFFYLVEKIVRREGIGDVLANGV
                     YEAARAIGKGAEQYDHNTMKKFEQVPLKLGMVNYPYFLMYATGEKMNITQIEGSYPQI
                     PVADMAEREEFVKHWHAAPERFKKWFLEWEPRSHPSVEAAVNICDWNETMHYVDDAIG
                     TCAFLSSFTGQWAGRAPYHIHNLPEFISLATGLDLDTDGLWEVARRNRNLIRAINVRR
                     GLRRSEEKPPEDHWKKREPEMEQHLLDEYYAFKGWTNDGIPTKETLDALGLGYVSEDL
                     IRRGILTGAESASTSAEPALAGAEGTVAK"
BASE COUNT          785 a         1650 c         1531 g         1032 t
ORIGIN      
        1 agccgccggg ccatgccgcc caggtcgtgg tccttctcca ccaagtagac ttcgaagccc
       61 tggttggcga cgctgagggc gctggtcatg ccggcgacgc cgcctcccac caccagcagc
      121 gccttgttca ccggcaggtc gtactcctcg aggggctcca ggcagcccgc ccgggcgacg
      181 gccatacgca cgatgtcctt ggccttcgcg gtggcagctt ccttctcgcg cgagtgcacc
      241 caggagcagt gctccctgat gttggcgaag tcgaagaagt actggttgat gccgccctca
      301 cgaagcgtgt cgcggaacag aggctcgtgg gtccgcggag tgcaggcggc cacgaccact
      361 cggttgagcc ccttctcgcg gatggtctcg gatatctgtt gcgcggcgtc ggtggaacag
      421 atgaagaggc cttcttccgc gtgcacgacg ttttccaagc ccagcgagta gtcgaccacg
      481 gagggcacgt ccaccacccg tccgatattg gccccgcagt gacacacgaa caccccgacc
      541 ttggtctctt cctcggagac gtcgcgctcg gtgggataga tcctgtcccg ggccagcttg
      601 ccgcgccggt aggcgaggag ttcgccgctc aacgaccccg ccccgctggc cgtaaccacc
      661 gactccggga tatccacggg tccgttgaag gcgccgctca cgaagacgcc tgggcgcgag
      721 gtcaggatgg ggttgaacgg gtcggtcttg cagaagccgt aggagtcgag ttccacgccg
      781 aacttggtcg ccatgtcctc ggcgtcggag ggggggttca tccccaccga tagcaccacc
      841 aggtcgaact cctcttcctt caccccgtcc tcggtggtgg agtacctgat ggtcacgttg
      901 ttggtctcgg ggatctccct ccccacggac acgtagctcc tgatgaaccg cactccggga
      961 aggttcgcgg ctctctggta gaagcgctcg aagtccttgc cgtaggatct gatgtcgttg
     1021 tggaacaccg tcgcttcgat gtcggcgtcg tggtccttgg agaggatcac ctgcttctgc
     1081 gtatacgagc agcacaccgc cgaacagtag gtgttgccgc ccgggatcac ctgtcgcgag
     1141 cccacgcagt ggatccaggc gatcttcttc gggtgcttct tgtcggaggg ccgcagggcc
     1201 tcgccctcga acgggccggt ggagcagagg agccgttcgt agtcgaggct ggtgacgacg
     1261 ttctgcatct ggccgtagcc gtaatcgccc ctcagccgcg ggtcgaacac ctcgtagccc
     1321 ggggacagca cgaccgcccc cacgttcacc acgaccttct cggccttctg gtgcaggtcg
     1381 atggccttgt tcttgcacac gcccacgcag atgttgcact tcttgtcttt gaggtagagg
     1441 cagtgctcct cgtccacgta ggtgaccagg ggcaccgcct gcgagaagtg tatgtggatg
     1501 gccttgttgt ccgacaagct ctggttgtac ttgtcgggca ccatcaccgg gcagtactcc
     1561 atgcaggtgt tgcagccggt gcacacgtct tctttgacgt atctgggctt cctgatcaga
     1621 gtcacgttga aatcgccggc ctcaccctcc acccgctcga cctcggtgta ggtcatgatc
     1681 tcgatgttgg ggtgccggtt gcactcgatg aacttcgggg attcgatgca catcgagcag
     1741 tcgttggtgg ggaaggtctt gtcgagctgc gacatcttgc cgccgatggt cggcgccttc
     1801 tccaccaggt agaccttgaa gccgtcggtg gccaggtcga gcgcggcttg gatcccgctg
     1861 atccccgcgc ccaccaccat gacgtcaccg agaccgtcgt ccggcaagat gctgaggctc
     1921 tgcccttggg ctgtgtcctt ttccacttct ctacatcctc atcaggcgtc tacggtcccc
     1981 tggagagact ccaggacgat ctccgttatg tccttgacca ccggagcgct gctctggtcg
     2041 gcgctcaacc tggcctcctc gaacatggtt atgcagtatg ggcaggacgt cgccagcacc
     2101 tcggcgccca cttccaccgc ctgcgccatc cggatgttcg agaaccgctc ctccttcggc
     2161 gtctccatcc agatgcggcc tccgccccct ccgcaacaca gactgtcgac ccgagaatcg
     2221 gacatctcca ccagctcgag accgggcacg ctccccagga gcgaccgggg ctcttcgtag
     2281 atgccgttgt gccggcccag gtagcacggg tcgtggtagg tgacccgctt cgcatactcg
     2341 tggctcaact cgagcctgcc ctgcttcacc agatcgagca gcagctccga gatgtgcacc
     2401 acctcgaagt gggcgtggaa gtcggggtat tcgttcttga aagtgtggta gcagtgcggc
     2461 gacgacacca ggatcttgct caccccggcg tcgatgaacg acttgatgtt gtcgcgggcc
     2521 aggcgcttga acacgtcctc gtcgccggtc ttgcggatgc tctcgccgca gcagctgtcc
     2581 ttggagccca ggatgccgta ctcgaccccg gccttgttca gcagcgtcgc cgtggccttg
     2641 gccaccttcg tcagacgcgg gtcgtagctg aggtagcagc tcgcgaagta caagaactcc
     2701 atgccctcgc tgaaggtctt gaccgagaga ccctgcgccc acttccccct gtcttcgcgt
     2761 ttctggccca gggggttgcc gtcgctgagg aggctcgccc tcgctgtgcg caccggctgg
     2821 acggccgtgg ggaacacctt gaactccgtg gcgatccgac ggagcgccac gcccgactcg
     2881 atctgcttca cgtcacgggg gcaccgctgc gggcatctgc cacaggtggt gcaacgccag
     2941 atctcctctt cttcgatctc cgtcatgccg aaggtcgcct cgcggacgat cttgcgcatg
     3001 ctgaaatccc tcaccaggtt ccacgggcag acgacgtcgc acagcccgca ctggtagcag
     3061 cgatcgaagg cggcgccgcc gttctcgtcg acgagttcac ggatctcggc ataatccgag
     3121 aggttctcag tctccattga ccttcctgtt cctacgcatg gccacgggtc tctagcccct
     3181 gctcgacatg cgggcgacag tgtccaggat cttgtccatt ccgaacttct tggccaacga
     3241 ttccaggccg atctccatct cgccgcgctt ctcttcctcc accggcttct cgtcttcccg
     3301 ctcggtgtag gtgaggcagt cgcatcggca cacctgcacg caccagggtt ccttcagagt
     3361 ggggtcttct tcgcacatgt cgcacttcag cggcaggcct gagtccggct cgtagaagta
     3421 gtctcgggac gggcaggaga ccccgcagaa gctgcactcg gagtactcct tgccgttgat
     3481 cgcgtatatg tgcctgccgg tgcactcggc cgacgtgttg tcgcccgccc gtatcggcac
     3541 atacaggtcg ctgagctcgt cgatgatcac ccggatacgc gacctggccg ggttcgtggc
     3601 gctgaacctg ggccgcgcgt ggaacgcgga acaggccacc tcgcaggcgc ggcagccgat
     3661 gcacttgtca aggtcgacct tgatctcctt gaccacccgc ttctcggccg tctcagtcat
     3721 gtggttctcg ctcacttggc caccgtcccc tcggccccgg ccaaggccgg ctcggcgctg
     3781 gtcgaggccg actcggcgcc ggtcaagatg ccgcgccgga tcaagtcttc actcacatag
     3841 cccaggccga gggcgtccaa cgtctccttg gtggggatgc cgtcgttggt ccagcccttg
     3901 aaggcgtagt actcgtcgag gagatgctgt tccatctcgg gttccctctt cttccagtga
     3961 tcctcgggcg gcttctcctc gctccgccgc aggccccgac gcacgttgat ggccctgatg
     4021 aggttccggt tgcggcgcgc gacctcccac agcccgtcgg tgtccaggtc gagcccggtg
     4081 gcgagcgaga tgaactcagg aaggttgtgt atgtggtagg gcgcccggcc ggcccattgg
     4141 cccgtgaacg acgagaggaa ggcgcaggtg cctatggcgt cgtctacgta gtgcatcgtc
     4201 tcgttccagt cgcagatgtt caccgccgct tcaaccgacg gatgcgaccg cggctcccac
     4261 tccaggaacc acttcttgaa cctctcgggg gcggcgtgcc agtgcttcac gaactcctcc
     4321 cgctcggcca tgtcggccac ggggatctgg gggtaggagc cctcgatctg ggtgatgttc
     4381 atcttctcgc cggtggcgta catgaggaag taggggtagt tgaccatccc cagcttgagc
     4441 ggcacctgct cgaacttctt catggtgttg tggtcgtact gctccgcgcc cttcccgatg
     4501 gcacgggccg cttcgtagac tccgttggcc aggacgtcgc cgatgccctc cctccgcacg
     4561 atcttctcca ctagatagaa gaacctcccg gccccgtcgg acgggaatcc gggcacatcc
     4621 tcgtcggtga ggatgccggc gtcgtagagt tccaccgcga aagccaggac ctggggcgtc
     4681 gagaacccgt ccagtccgta ctcctgggtg aggccgagga tgtcgtagtt gaagtccagc
     4741 tcttcgtagg ccgccatggc gtaggtgagc ttgctgtagc acttctggaa atacggcatg
     4801 cggccgggat aggtgatcgc ctggtggcaa tccttcggac agttgtagca gccggtccag
     4861 cgcacccggg cgttgtccga gatctccttc cacctctcct cgcgctcgtg gttccagtag
     4921 cccttgatgc gatcgcgcgc gttgccccag gcgaagttgt tcacgtggaa ctcttcgtcg
     4981 tcctcgtggg cgaggagg
//
LOCUS       DASRMJ010000268         6681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_202827, whole genome shotgun sequence.
ACCESSION   DASRMJ010000268 DASRMJ010000000
VERSION     DASRMJ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202827"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VFD74_07345"
     CDS             <1..1225
                     /locus_tag="VFD74_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZK49397.1"
                     /translation="DILPFPKNLLYPLVKHGDVASVPRDAQHATFAELIHNDAEYAPV
                     EVQSQDLAVLQYTGGTTGVPKGAMLTHANLYANAYQCLKWFPGVVQGWERMLVVLPLF
                     HVFAMSAAMNMAVLMAAEMVLLPRFEVQQVLHTIDKNHPTLFPGVPTMYTAINGRQDL
                     DKYDLSSIKYCISGGAALPVEVKKTFERLTGCILVEGYGLTETSPVATCNPLTGENKA
                     GSIGLPVPQTVIEIRSLEEPAAKMPLGEKGEICIKGPQVMPGYWQNQEATDAVLKDGF
                     LHSGDVGYMDDDGYTFIVDRMKDMILSGGFNVYPRNIEEAVYEHPAVSEVVCIGIPDE
                     YRGECPKVFYTVKPGVTVGPDELKEFLKERLSKVEMPRDIEQRETLPKTLVGKLSKKE
                     LVEEERTKGDLTWRV"
     gene            1213..2349
                     /locus_tag="VFD74_07350"
     CDS             1213..2349
                     /locus_tag="VFD74_07350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZK49398.1"
                     /translation="MEGVVIAGYVRSPMTPANKGELAKVRPDEIARQVIRGLVERTGL
                     DVDDVEDLLLGCSSPEGEQGLNLARLVGFLADLPVSMAAVTINRFCGSSMQAIHTAAG
                     AVQMNAGECFICAGVESMTRVPIWGFNPMPHPGLHASYAEAYTSMGMTAENVARIYGI
                     SREEQETFAVESHRKAAQAAAGGRFDDEIVSIRDGDRVISRDGCIRPGTTAEALAGLK
                     PVFLAGGTVTAGTASPLTDGASAVLVCSERYAREKGLDMLARIRSVAVSGCSPEIMGM
                     GPVASTTKALQRAGLTMDDMDVIEINEAFASQSLAVVKELGVDQAKLNLDGGAVALGH
                     PLGASGARVTGKAASLLKREGARYALATMCIGGGQGIATVLEAI"
     gene            2350..4722
                     /locus_tag="VFD74_07355"
     CDS             2350..4722
                     /locus_tag="VFD74_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZK49399.1"
                     /translation="MGAASEAGGIRRAAVIGAGLMGAGIAAHLANAGAEVLLLDIVPS
                     DLPEGASRSVLAETAVAKLLKADPAAFMHKRNARLITPGNLDDHLDRLAEVDWIIEAV
                     VERLDIKQQVYARIDAVRRPGSAVSSNTSTIPLVRLVEGASEQFARDFMITHFFNPPR
                     YMRLLELVVGEKTDPATAQMLRSFADERLGKGVVDANDTPGFIANRIGGYFLQCAMVE
                     AMDSGLSVEEADAVLGRPMGAPKTGIFGLLDLVGLDTVRHVGRSLFENLPADDPFHAV
                     HREPELVTRLIEAGYTGRKGKGGFYRLNTEGGGRVKEAVDLATGDYHPAAKPRLGSVD
                     AARRGGLRALVESPDPGGAYAWRVLSRTLSYAAALVPSVAADITAVDEAMRLGYNWKK
                     GPFELIDELGAAWFAERLAEDGQAVPTLVALAAAGSTAAGAFYRVAEGNLQHLTPDGG
                     YADVRRRPGVLLLADVKRRGERIAGNSSASLWDLGDGVACLEFHSKMNAFDDGTLKMI
                     GTAIETVMMSGFAGLVLYSDGDNYSVGANIGLGLFAANIGLWPAITELVEGGQQAYRF
                     LKYAPFPVVAAPAGLALGGGCESLLHSDAIQAHAESYIGLPEAGLGLLPSWGGTKEML
                     LRHTQNRKRMHGPVPPIAGAFETISMAKVSRSAEEARELLFLRGDDGVTMNRDRLLAD
                     AKAKVLELAVSYAPPEEPTFKLPGPSGRAALEIFVGEFVNSGKATAHDAVVSGQIAVV
                     LTGGDADITEEVSEERLLELEREGFLALIHEPKTLARVEHMLETGRPLRN"
     gene            4727..6589
                     /locus_tag="VFD74_07360"
     CDS             4727..6589
                     /locus_tag="VFD74_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZK49400.1"
                     /translation="MTIYKAPLRDIRFILNELVHAERLVDLPGYEEATLDQIDFILEE
                     IAGICENLLFPLSRIGDEEGCRFENGTVTTPPGFKEAYAKYVEAGWPALACDPEFEGM
                     GLPKLVSFLGEEVLSASNLAFAVYPLLTTGAYTAIHKHGSDELKRKYLPKMVSGVWCG
                     TMCLTEAHCGTDLGLVRTRALPQDDGSYRVTGTKIFISSGDHDFTENIIHLVLARLPD
                     APPGIKGISLFLVPKIRVEEDGSLGAGNGVTCGGIEKKMGVAGSSTCVLNFDDAVGYL
                     VGEKHRGMSHMFTMMNAARLGVGMQGLGIAEVAYQNAVAYARDRVQGRALKEPRYPER
                     EADPLIVHPDVRRMLLTMRAVTEGGRALAGWVGLNLDLAERHPDPQVREEAGDLVDLL
                     TPVVKAFFTDHGFAAANMGVQIHGGHGYIRETGVEQYVRDVRICQIWEGANGIQGLDL
                     VGRKLGLHNGRLLRRFFHPVAAYLEARQTDARLGEFVAPVAKAFGRLQQATARIAQQG
                     LRDPDEAAAVASDYLRLFALVALGYMWLQMAEISLGRLGIGVGAAAGATAGSAASAVA
                     AIAVATDPKDLVFYRGKLATARFFFQKLLPESSSLFAAIMAGAAPVMALDDKEF"
BASE COUNT         1147 a         2198 c         2261 g         1075 t
ORIGIN      
        1 cgacatcctg ccgttcccca agaacctgct ctacccccta gtcaagcacg gtgacgtggc
       61 ttccgttccg cgcgacgcac agcacgccac cttcgccgaa ctcatccaca acgatgcaga
      121 gtacgcgccc gttgaagtgc agagccagga cctcgcggtg ctgcagtaca caggcggcac
      181 caccggggtg cccaagggtg cgatgctcac ccacgccaac ctctacgcca acgcctatca
      241 gtgcctcaag tggttcccgg gcgtcgtgca gggctgggag cgcatgctcg tggtgttgcc
      301 gctgttccac gtgttcgcca tgtcggcggc catgaacatg gcggtgctga tggcggccga
      361 gatggtgctg ctgccgcgat tcgaggtgca gcaggtgctg cacaccatcg acaagaatca
      421 ccccacgctc ttcccgggcg tgccgacgat gtacacggcg atcaacgggc ggcaagacct
      481 cgacaagtac gacctctcgt ccatcaaata ctgcatctca ggcggcgcgg cactgcccgt
      541 cgaggtcaag aagaccttcg agcggctcac gggatgcatc ctggtggagg gatacgggct
      601 taccgagacg tctccggtgg ccacctgcaa ccccctaacg ggcgagaaca aggccggctc
      661 catcggactc ccggtgccgc agaccgtcat cgagatccgc tcgcttgagg aaccggcggc
      721 gaagatgcct ctcggcgaga agggcgagat ctgcataaag ggtccgcagg tcatgcccgg
      781 ctactggcag aaccaggaag ccaccgacgc ggttctgaag gacggattct tgcactccgg
      841 cgacgtcggc tacatggatg atgacggcta caccttcatc gtcgatcgga tgaaggacat
      901 gatcctctcc ggcgggttca acgtgtaccc gcgcaacatc gaggaggccg tctacgaaca
      961 tccggccgtc tcggaagtcg tgtgcatcgg tatccccgac gagtaccggg gcgagtgccc
     1021 caaggtgttc tacacagtga agccgggagt aacggtgggt ccggacgagc tcaaggagtt
     1081 cctcaaggag cgactctcca aggtggagat gccgcgggac atcgaacagc gcgagacgtt
     1141 accgaagacg ttggtgggca agctctccaa gaaggaactg gtggaagagg agaggacgaa
     1201 aggggatctg acgtggaggg tgtagtcatc gccgggtacg tgaggtcacc gatgaccccg
     1261 gcgaacaagg gtgagctggc caaagtccga cccgacgaga tagcccggca ggtgatacgc
     1321 ggactcgtcg agcgcacagg cctggacgtc gacgacgtgg aggacctgct cctgggatgc
     1381 tcgtcgcccg aaggcgagca ggggctcaac ctggcccggc tcgtgggatt cctggccgat
     1441 ctccccgtct cgatggcggc cgtcaccatc aatcgttttt gcggatcgtc gatgcaggcg
     1501 atccacacgg cggcgggggc ggtgcagatg aacgcaggcg agtgcttcat ctgcgcgggg
     1561 gtcgaatcca tgacccgggt ccctatctgg gggttcaacc ccatgccgca cccggggctg
     1621 cacgcgtcgt acgccgaggc gtacacgtcc atgggcatga ccgctgagaa tgtggccagg
     1681 atctacggga tatcgcgaga ggagcaggag accttcgcgg tcgagagtca ccgcaaggcg
     1741 gcccaagccg ccgcgggagg ccgcttcgac gacgagatcg tgtccatccg agatggagac
     1801 cgcgtgatct cgcgcgacgg ctgcattcgg ccgggcacga cggcggaggc cctggcggga
     1861 ctcaagcccg tcttcctggc cggcggcacc gtgacggcag gcacggcctc gcccctcacc
     1921 gatggagcgt cggccgtgct cgtctgctcc gagcgctacg cgcgcgagaa gggactcgac
     1981 atgctggccc gcatccggtc cgtcgcggtg tcgggctgct cacctgagat catgggcatg
     2041 ggaccggtgg cctccacgac caaggctctg cagcgggccg gcctcacgat ggacgacatg
     2101 gacgtgatcg agatcaacga ggcgttcgcc tcccagtccc tggccgtggt gaaggagttg
     2161 ggcgtcgatc aagccaagct gaacctggac ggcggagcgg tggccctggg ccatccgctc
     2221 ggcgccagcg gggcccgcgt caccggcaaa gcggcgtcgc tgctgaagcg cgagggtgca
     2281 cgctacgctc tggccacgat gtgcatcggc ggcggtcagg gcatcgccac cgtgttggag
     2341 gcgatctaga tgggcgccgc gtctgaggcc ggcggcatac gccgcgcggc ggtcatcggg
     2401 gccgggctta tgggggccgg catcgcggcg cacttggcca acgccggcgc cgaggtgctt
     2461 ctcctcgaca tcgtgccttc cgacctacct gaaggcgcct cgcgcagcgt cctggccgag
     2521 accgcggtgg cgaagctgct caaggcagac cccgccgcct tcatgcacaa gcggaacgcc
     2581 cggctcatca ccccaggcaa tctcgacgac catctcgatc gtttggccga ggtcgactgg
     2641 atcatcgaag ccgtggtcga gcgactcgac atcaagcagc aggtgtatgc gcgtatcgac
     2701 gcggtccggc ggcctggttc cgccgtgagt tcgaacacct ccaccattcc cttggttcga
     2761 ctggtggaag gcgcttccga gcagttcgcg cgcgacttca tgatcaccca cttcttcaat
     2821 ccaccgcgct acatgcgcct gctggagctg gtggtggggg agaagaccga tccggccacg
     2881 gcccagatgc tgcgctcgtt cgccgacgaa cggttgggca agggcgtggt cgacgccaac
     2941 gacaccccgg gcttcatcgc caaccgcatc gggggctact tcctgcagtg cgccatggtg
     3001 gaagccatgg actccggctt gagtgtggag gaggccgacg cggtgctggg gcgccccatg
     3061 ggcgcgccca agaccggcat cttcgggttg ctcgacctgg tgggcctcga caccgtgcgg
     3121 cacgtcggtc gaagcctgtt cgagaacctg cctgcggacg atcccttcca cgccgtgcac
     3181 cgggagcctg agctggtgac ccgcctcatc gaggccggct acaccggacg caagggcaag
     3241 ggcggtttct accgactgaa caccgaagga ggaggcaggg tgaaggaggc cgtggacctt
     3301 gccacgggcg actaccatcc cgccgccaaa cctcgcctcg ggagcgtcga cgccgcccgc
     3361 cggggtggat tgcgggctct cgtcgagtcg cccgacccgg gcggcgccta cgcgtggcgg
     3421 gttctcagtc gcaccttgag ctatgccgcc gcgctggtcc cgtccgtggc cgccgacatc
     3481 accgccgtgg acgaggccat gcgcctcggc tacaactgga agaagggtcc tttcgagttg
     3541 atcgacgagc tcggtgcggc ctggttcgcc gagagactgg cggaggacgg gcaggcggtg
     3601 ccgactctgg tggcactggc ggcggccggc tctaccgccg ccggtgcgtt ctaccgcgtc
     3661 gctgaaggca acctgcagca cttgacgccg gacggcggct acgccgacgt ccggcgtcgt
     3721 ccgggcgtgc tcctgctggc cgatgtcaag cgccgcggcg agaggatcgc cggcaactcc
     3781 tcagcgagtc tgtgggacct gggcgacggg gtcgcctgcc tcgagttcca ctcgaagatg
     3841 aacgccttcg acgacggcac cctcaagatg atcggcaccg ccattgagac cgtcatgatg
     3901 tccggcttcg ccggtctcgt gctctacagc gacggcgaca actactcggt gggcgccaac
     3961 atcggcttgg gcctcttcgc cgccaacatc ggtctgtggc cggccatcac cgagttggtg
     4021 gagggcggcc agcaggccta tcgtttcctc aagtatgctc cgttccccgt agtggcggcg
     4081 cccgccgggc tggccctggg tggcggctgc gaatcgctgc tgcatagcga cgcgatccag
     4141 gcccacgccg agagctacat cggcctgccg gaagcgggtc tgggtctgct gccgtcgtgg
     4201 ggcggcacca aggagatgct tctgcgtcac acgcagaacc ggaagcgcat gcacggcccg
     4261 gtgcccccta tcgcgggggc ctttgagacc atcagcatgg cgaaggtgtc gcggtcggcg
     4321 gaggaagcgc gcgagctgct cttcctgcgt ggggacgacg gggtcaccat gaaccgcgac
     4381 cggctcctgg ccgacgcgaa ggccaaggtg ctggagctgg cggtctccta cgcgccgccg
     4441 gaagagccca cgttcaagtt gcccggaccg tcgggtcggg cggcgctcga gatattcgtg
     4501 ggcgagttcg tcaactcagg gaaagccacc gcccacgacg cggtcgtgtc ggggcagatc
     4561 gccgtcgtac tgacgggagg agacgccgac atcacggagg aggtctccga ggagcgcctc
     4621 ctcgaactag aacgtgaggg cttcctggcg ctcatccacg aaccgaagac ccttgcccgg
     4681 gtcgagcata tgctcgagac cggccgaccg ttgaggaact gaggccatga ccatctacaa
     4741 ggctcccctg cgcgacatcc gcttcatcct gaatgaactc gtccacgccg agcgactcgt
     4801 cgacctcccc ggctacgagg aggccacgct cgaccagatc gacttcatac tcgaggagat
     4861 agccggcatc tgcgagaacc tgctcttccc cttgagtcgc atcggtgacg aggaggggtg
     4921 tcgcttcgag aacgggaccg tcaccacccc gcccggattc aaagaggcct acgcgaagta
     4981 cgtggaggcc ggctggccgg ccctggcctg cgaccccgag ttcgaaggga tgggccttcc
     5041 caagctcgtg agcttcctcg gagaggaggt gctctccgcg agcaacctgg cgttcgccgt
     5101 ctacccgctg ctcaccaccg gtgcctacac ggccatccac aagcacggct ccgacgagct
     5161 gaagcgcaag tatctgccca agatggtctc gggggtatgg tgcggaacca tgtgcctcac
     5221 cgaagcccat tgcggcaccg accttgggct ggtgcgaacc cgggcactgc cacaggacga
     5281 cggctcctac cgcgtcaccg gcaccaagat cttcatcagt tcgggtgacc acgacttcac
     5341 tgagaacatc atccacctgg tgctggcccg tctgcccgac gcgcctccgg ggatcaaagg
     5401 catcagcctg ttcttggtgc ccaagatacg cgtggaggag gatgggtcgc tcggcgccgg
     5461 caacggcgtg acctgcggcg ggatcgagaa gaagatgggg gtggcggggt cgtccacctg
     5521 cgtgctcaac ttcgacgatg ctgtcggcta cctggtgggc gagaagcacc ggggcatgag
     5581 ccacatgttc accatgatga acgcggcgcg cttgggcgtg ggcatgcagg gcctcggcat
     5641 agccgaggtc gcctaccaga acgccgtcgc ctacgcccgc gaccgcgtgc aggggcgggc
     5701 gctcaaagag ccccgctacc cggagcgcga ggccgacccg ctcatcgtgc accccgacgt
     5761 ccgtcgcatg ctcctgacca tgcgggccgt caccgaaggc ggtcgcgccc tcgccggatg
     5821 ggtggggctc aacctcgacc tggccgaacg ccatcctgac cctcaggtgc gcgaggaggc
     5881 cggtgacctg gtcgacctgc tgactccggt ggtcaaggcc ttcttcaccg atcatggctt
     5941 cgccgccgcc aatatgggcg tgcagatcca cggtgggcac ggctacatcc gcgagacggg
     6001 cgtggagcag tacgtgcgcg atgtccgcat ctgccagatc tgggagggcg cgaacggcat
     6061 ccaaggactc gacttggtgg ggcgtaagct cggcctgcac aacggccgcc tgctgcgacg
     6121 cttcttccac ccggtggctg cgtacctgga ggcgcggcag acggacgccc gcctcggcga
     6181 gttcgtcgcg ccggtggcca aggctttcgg acggcttcag caggcgacgg cgcgcatcgc
     6241 ccagcagggc ctgcgcgacc ccgacgaagc cgccgccgtg gcgagcgact acctgcgact
     6301 gttcgccttg gtcgccctcg gatacatgtg gctgcagatg gccgagatca gcctggggcg
     6361 actgggcatc ggggtgggcg ccgccgccgg cgcgaccgcc ggatctgccg catccgccgt
     6421 cgccgcaatt gccgtggcca cggatcccaa agacctggtc ttctaccgag gcaagctggc
     6481 cacggctcgc ttcttcttcc agaagttgct gccggagagt tcatcgctct tcgccgccat
     6541 catggccggg gcggcgccgg tgatggcgtt ggacgacaag gagttctagc acggccgcgc
     6601 ccccgtgtcc ccgcgccaac gtgggcgcgg ggacacgggg gcgcggccgt gctagaactc
     6661 cttgtcgtcc aacgccatca c
//
LOCUS       DASRMJ010000269        11224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_531300, whole genome shotgun sequence.
ACCESSION   DASRMJ010000269 DASRMJ010000000
VERSION     DASRMJ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11224
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531300"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFD74_07365"
     CDS             complement(<1..343)
                     /locus_tag="VFD74_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008309417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZK49401.1"
                     /translation="MRLTSSTGSSPHDCDGAATADVVLLDGALVPRGEATVSAFDRGV
                     MYGESLFETLKVVDGAPCLWDLHRDRLTRGCEESGIPLDSDSVWRGVARLLARRPVSH
                     GVLRVQVTGGAQ"
     gene            complement(315..1877)
                     /locus_tag="VFD74_07370"
     CDS             complement(315..1877)
                     /locus_tag="VFD74_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HZK49402.1"
                     /translation="MTTGPGSGVGFGSSTDAAADERWFGRVLGPYAYRPIELMERLDL
                     IPRGGGQTGAGDPPLDPSRLSVLLDSAGGNPNLARRSLLAWDPEAHLWAKDGRVALCR
                     LDGGGTWVRDDTRGDPFPVLRDALRDRRRPITWLGPDEGEPEWVGAYGLLTYDAGRYI
                     EVLPDDTLDDLRIPDVDVIFPRKWVCFDHERGRVTAVAHSPEALEEIEEYLSRAARAP
                     RVHLDLSVDGHSREGSLRTGSNLTRAQYEAMVERAREYVWAGDIFQTNLSQRLELRYE
                     GESSLLYRVLRRVNPSPFAGYLRLSGYELFSSSPERLVRLRGRVADTRPIAGTRRRGR
                     DFTEDGVLADDLNLDPKERAEHIMLVDLERNDLGRVCEYGSVHVDELMVNERYSHVIH
                     IVSNVTGTLSASRDAVDLLRAMFPGGTITGCPKVRCMEVIDELETVRRGPYTGSFGFF
                     GYNGDMDTNIVIRTLARVGGRLYAQAGGGVVADSVAGNEYMESLSKAEAMVGAALEAG
                     RLAGEAHEQHGE"
     gene            complement(1874..3292)
                     /gene="dnaB"
                     /locus_tag="VFD74_07375"
     CDS             complement(1874..3292)
                     /gene="dnaB"
                     /locus_tag="VFD74_07375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZK49403.1"
                     /translation="MGDNGPGRAGSKTDYVPPQNLDAEVSVLGALMVAPNLIPGVTEV
                     LRPVHFYRRSHQYIYEVIEDLYGRGEPVDPIMVSEALSNQARLEEVGGRAFVHSMVSA
                     VPAATNARYYAEIVRENYMLRSLIEVGTGIAELGYRREGEPVDLIDRAEQMVFDISQN
                     RVSSEFHRLSDLISENFAELEKIMSEGRRTTGVETGFRRLDEKITGFQPANLVILAAR
                     PSMGKTALALNIARNVAVDQGKGVALFSLEMSRMELVQRMMCSEARVDSHRLRHGLLQ
                     PAEWSKLTAACTPLFTAPIYIDDTASVNLMEIRAKSRRLKAKEKGLGLIIIDYLQLMM
                     GDASTENRQQEISRISRGLKILARDLDIPVLALSQLSRQVEQRAGNKRPVLSDLRESG
                     AIEQDADLVLFIYRDEVYNKDSADKGIAEIVIGKQRNGPIGEAKLAFIERYTRFSDMQ
                     SDQRPDAARRPNDREGAGWERS"
     gene            complement(3343..3801)
                     /gene="rplI"
                     /locus_tag="VFD74_07380"
     CDS             complement(3343..3801)
                     /gene="rplI"
                     /locus_tag="VFD74_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZK49404.1"
                     /translation="MEVILRQDVDKVGRKGGVANVSDGYARNFLLPQKLAEVATASRI
                     AAVRTAQEERDARERREAAQAEDSRDMLSKTVLTIPAPVGSGDRLFGSITSQDIATAI
                     FQARKVRIDKRNVHLEDAIKAVGTYMVKVDVHASVEPAEVKVIVVPEEKK"
     gene            complement(3802..4821)
                     /locus_tag="VFD74_07385"
     CDS             complement(3802..4821)
                     /locus_tag="VFD74_07385"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HZK49405.1"
                     /translation="MSSFSTGATSEAGKAVALAVVLSALIAYVPVLGAVLMPVLPMPA
                     AYVTFRRGVVVGVAAAFGAGVLSAALTGLGNGLLTFLLSALVGVVVGWALARRLGFSA
                     TLLATTVGGAVAFAVSTVAAWVLAGLDRTQIVKLVDDSLAAASRLYSSMGVDPAVIES
                     ASRQIREWLDIVPYLLPSILGVAGLIMASATLALVAVVFPHLGEKAASGLAFSRFRLH
                     WSASYGFIVGLGLMALGPALGAWAEAARLIGLNFFMFFETLFFIQGLSVAHWFVVTRR
                     HGAGVRIAVYGGAVLGQLLLQITSWAGLLDTWFDYRKRFAPRAPKAGSAGPAGSRGTG
                     DQEES"
     gene            complement(4864..5115)
                     /gene="rpsR"
                     /locus_tag="VFD74_07390"
     CDS             complement(4864..5115)
                     /gene="rpsR"
                     /locus_tag="VFD74_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZK49406.1"
                     /translation="MAFAKRRRTRPTTGNRRRKYCYFCKEKIQHVDYKDFNQLRRFTS
                     ERAKIRSRRTTGACRRHQRQLAVAIRRAREMALLPYSNR"
     gene            complement(5138..5626)
                     /gene="ssb"
                     /locus_tag="VFD74_07395"
     CDS             complement(5138..5626)
                     /gene="ssb"
                     /locus_tag="VFD74_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZK49407.1"
                     /translation="MARGIAHVTLVGNLTRDPELKQLPSGTSLCKLGVAVNSSVKDSS
                     GQWQEKANFFDVTVWGQQGENCARYLAKGRQVAVDGRLNYDSWEAQDGSKRSRVDIIA
                     DTVMFLGGANEGSGDSGSSGGAGGGRSNRASAPGSGSNSGDADFRDIDFSKIDDEDDI
                     PF"
     gene            complement(5630..5980)
                     /gene="rpsF"
                     /locus_tag="VFD74_07400"
     CDS             complement(5630..5980)
                     /gene="rpsF"
                     /locus_tag="VFD74_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZK49408.1"
                     /translation="MTELNSYELMLILRPDTVDEARAAILDRIKEIVAADQGVMGKID
                     EWGKRRFAYEIAHMTEGYYYVMYFNVEPKTLDEITRVLGITDAVVRNMPVRLEDPVAP
                     VAAVAAVAATTTEE"
     gene            complement(6118..6405)
                     /locus_tag="VFD74_07405"
     CDS             complement(6118..6405)
                     /locus_tag="VFD74_07405"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HZK49409.1"
                     /translation="MVLLFHNTQSALRAEEILLEAGLEVDVVPRPEGVPGGLCGIALD
                     VSAARLAEVDRLLCADGVAYDLHVGGFGIEAQECAPVAAGEEDGLLPGPPG"
     gene            complement(6499..7650)
                     /locus_tag="VFD74_07410"
     CDS             complement(6499..7650)
                     /locus_tag="VFD74_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZK49410.1"
                     /translation="MSGTGSSFPKDALSRAARLQRAALQGPAEFGFRMRWAPALALAL
                     LLGLLIAGCGGDSPTRDVAGSPATTIYASDSGAPTLPGSGDQFSNKSGSGAPLMGESG
                     TAGIFETSMSLDRKIVSNATLLVEVERGQFQIAFDQALLLADKFGGYVVSSNSTAADA
                     ESTMRSGTIALRVPETAFTQALSDAAKLGTVKSRNVDTQDVTEEYLDLGARLKNAEVQ
                     EKAFLALMDKAKTIDEILQVRQVLSQTQMEIEQLKGRIRFLDEHTSFSTLTISLYEAG
                     SEFKPAGSWGFVDSLKDALHAFVDSVNEIIVFMGGALPVLVLLVLLAWLGYRLIRLLT
                     ARTSGGRGHQRHHDDAGPAQPAAAPAAASAVAAVEESKSAPTPPSDPQG"
     gene            complement(7742..8524)
                     /locus_tag="VFD74_07415"
     CDS             complement(7742..8524)
                     /locus_tag="VFD74_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HZK49411.1"
                     /translation="MKIAITGKGGVGKTTLAGTMARIFASDGYKVLAVDADPDANLAS
                     ALGIDADVARAIVPIADMKELIAERTGSTPGTMGGFFKINPRVDDIPDELSVPMDGIR
                     LMVMGTVKEGAGGCVCPESVLLRSLIRHLLLRRKEVVILDMEAGIEHLGRGTAEGVDA
                     MIVVVEPGARSARTADSIRRLASDIGIKRLFVVLNKVDPSERAQMEAMLEGFHILGAL
                     PYSAGVRRADLDEAPPFERAPEYVEAVRGIVQQVRSALEVED"
     gene            complement(8521..8859)
                     /locus_tag="VFD74_07420"
     CDS             complement(8521..8859)
                     /locus_tag="VFD74_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49412.1"
                     /translation="MARKAHGPRLDLIGGLDSVGSRLAGRANERLERSPVRAPARLLF
                     AGVVMLISASVLAAASAGEPGSADSPGQVAVVVATLLYAGGGLSLVIGLAGLLSALVV
                     HILGMKGRSR"
     gene            complement(8917..9441)
                     /locus_tag="VFD74_07425"
     CDS             complement(8917..9441)
                     /locus_tag="VFD74_07425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZK49413.1"
                     /translation="MLEDNTQHIAIKRHARLTSHPAGRMNSMWDWRSRVPLRRVAVNF
                     AVVYACRYLPFLRWKLMLLRAIGIHIGPFAAPGLGSTFDVFYPQLISVGANTIIGYNT
                     VILAHEFLREELRTGPVVIGRDVVIGANCTILAGVVIGDGAVVSAHSLVNADVGAGAR
                     VGGVPARPLRGTKE"
     gene            9533..>11224
                     /locus_tag="VFD74_07430"
     CDS             9533..>11224
                     /locus_tag="VFD74_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZK49414.1"
                     /translation="MALNSAYTHTVAPPYEPEHARPFQANLARGVALWSTLAGALVVT
                     LLAAGWLVGLREIDTAPPAIALGFFFLALAPIGRYIVKNEDSWSHSRDMKVAGLLVTL
                     DIGTGAFVLFWTGGPESVLIVLVPLLPFALRLLGNIWYSVAYTVAIAAGFAIMIIGER
                     LGFLRHAASLLDAAVISHGQATGLLVFTTTATVVSATILADAIAKIVTRRETEAIAFS
                     EKMNTRAEELALLLRIGRVLSRSADFEVAAREALDHVHAHFRPAATVLYLKDPHTGLL
                     AVLSSLGEWAGDERSETTLASTALDAREAHMYPEAAAGGRSRHSTMIAPLLVEDTGYG
                     AIKVVGQPGRTFDRSKLALLETIASELAATVRSASNYQSANAELTRAGAELAALNTFT
                     RSVSSSLDLDNICHNLLSTAMRLTGADSGLVTILSSDGNADISIFRNYEPTTERLLRS
                     TDWRRSRGLFGRALRTGRPVVVGDVSLDSEYESIVPAVRSKVCVPIVVQGDVAGMVSL
                     ESRATDAYSPNDVDFLAAMAESAAVAVKNARLYRTLEESAIRDGLTGLYNHSYFHQ"
BASE COUNT         1917 a         3899 c         3500 g         1908 t
ORIGIN      
        1 gctgcgctcc gccggtcacc tgcaccctca gcacgccgtg gctcaccggt ctccgcgcca
       61 ggagccgggc cactcctctc cagacggagt cggaatcgag ggggatgccc gattcctcgc
      121 aaccccgggt cagacggtcg cggtggaggt cccacaagca aggagcgccg tcgaccacct
      181 tgagcgtctc gaagaggctc tcgccgtaca tgaccccgcg gtcgaacgcc gagaccgtgg
      241 cctctccccg cggcaccaag gccccgtcga gcaggaccac atccgcagtg gccgcgccat
      301 cgcagtcgtg tgggctactc cccgtgctgc tcgtgagcct caccggccaa tcgacccgcc
      361 tccagcgccg cgcccaccat ggcctcggcc ttgctgagcg actccatgta ctcgttcccg
      421 gcgacggagt cggccaccac gccgcccccg gcctgcgcgt acaacctgcc cccgacgcgc
      481 gccaaggtgc ggatgacgat gttggtgtcc atgtcgccgt tgtatccgaa gaagccgaac
      541 gagcctgtat aggggccgcg acgcaccgtc tccagctcgt cgatgacctc catgcaccgc
      601 accttagggc aacctgtgat agtccctccc gggaacatcg cccgcagaag gtcgacggcg
      661 tccctcgagg cggacagcgt gccggtgacg ttgctcacga tatggatgac gtgactgtag
      721 cgctcgttca ccatcaactc gtccacgtgc accgagccgt actcacacac ccggccgaga
      781 tcgttgcgct ccaggtcgac gagcatgatg tgctccgcgc gttccttggg gtcgagattg
      841 aggtcgtcgg ccaagacccc gtcctcggtg aaatcgcggc cgcgacggcg tgtgccggcg
      901 atgggccggg tgtcggccac tctcccccgt agcctcacca gccgctcggg cgagctggag
      961 aagagctcgt atcccgacag acgcaggtac cccgcgaacg gcgaggggtt cactctgcgg
     1021 agcacccggt acagcagaga ggattcaccc tcataccgaa gctcgaggcg ctgcgacagg
     1081 ttggtctgaa agatgtcgcc ggcccacaca tactcccggg cacgctccac catggcttcg
     1141 tactgcgcac gcgttaggtt cgagccggtc ctcaggctcc cctctcggct gtgtccgtcg
     1201 acggagaggt ccaggtggac cctcggggcg cgggcggccc gggacaggta ctcctctatc
     1261 tcctccaacg cctcgggcga gtgggccacc gcggtcaccc ggccgcgctc gtgatcgaag
     1321 cacacccact tccgcgggaa gatgacgtcg acatcgggta tacggagatc gtccagcgtg
     1381 tcgtcgggta acacctcgat gtagcggccg gcgtcgtagg tgagcagacc gtaggccccc
     1441 acccactccg gctccccctc atcgggcccg agccaggtga tgggacgccg ccggtcccgg
     1501 agtgcgtcgc gcaacaccgg aaagggatcg cctcgggtgt cgtcacgcac ccaagtcccg
     1561 cccccatcca ggcggcagag cgcgacacgc ccatccttgg cccacaggtg ggcctccgga
     1621 tcccaagcca agagcgatct tcgcgcgagg ttgggattgc cgcccgcgct gtccaggagc
     1681 acggagagcc gcgagggatc gaggggaggg tcccctgcgc cggtctgccc ccctccccgg
     1741 gggatcagat cgagacgctc catgagttcg atgggacggt acgcatatgg tccgaggacc
     1801 cgcccgaacc accgttcgtc cgccgccgcg tccgtcgatg agccgaaccc gacgccggac
     1861 cccggaccgg tcgtcacgac ctctcccagc cggcgccttc ccggtcgttg gggcgtcggg
     1921 ccgcgtcggg acgctggtcc gactgcatgt cggagaagcg cgtgtagcgc tcgatgaagg
     1981 ccaacttggc ctcgccgatg ggaccgttcc gttgcttgcc gatgacgatc tcggcgatgc
     2041 ccttgtcggc gctgtccttg ttgtagacct cgtcccggta gatgaagagg acgaggtcgg
     2101 cgtcctgctc gatggccccc gattccctga ggtcggagag gacgggacgc ttgttcccgg
     2161 cacgttgctc cacctgccgc gagagctgcg aaagtgccaa gacggggatg tccaggtcgc
     2221 gcgcgaggat cttgaggccc cgtgagatgc gtgagatctc ttgctgccgg ttctccgtgc
     2281 tcgcgtcgcc catcatcagc tgcaggtagt cgatgatgat caaccccagc cccttctcct
     2341 tcgccttcaa gcgcctggac ttcgcgcgta tctccatgag gttcacggaa gccgtgtcgt
     2401 cgatgtagat gggggccgtg aagagtgggg tgcaggcggc cgtcagcttg ctccactcgg
     2461 ccggctgaag gaggccgtga cgcaggcggt ggctgtcgac gcgggcctcg gagcacatca
     2521 tccgctgcac cagctccatc ctcgacatct cgaggctgaa gagggccacg cccttgccct
     2581 gatccaccgc gacgttccgc gcgatgttca gagccaaggc ggtcttgccc atggaggggc
     2641 gggcggccag gatcaccaga ttggccggct ggaagccggt gatcttctcg tccaaacgcc
     2701 ggaacccggt ctccaccccg gtggtccgtc gcccctcgga cattatcttc tcgagctcgg
     2761 cgaagttctc gctgatcaag tcgctcagcc ggtggaactc ggatgagacc cggttctgcg
     2821 agatgtcgaa caccatctgt tccgctcgat cgataagatc gaccggctcg ccctcgcgcc
     2881 gatagccgag ttcggcgatg ccggtgccca cctcgatgag cgaccgcagc atgtagttct
     2941 cgcggacgat ctcagcgtag tagcgggcgt tggtggccgc tgggaccgcc gacaccatac
     3001 tgtgcacgaa cgcgcgcccg cccacttctt cgaggcgcgc ttgattgctg agcgcctcgc
     3061 tcaccatgat ggggtccacc ggctcgccac gaccgtagag gtcttcgatg acctcgtaga
     3121 tgtactggtg gctgcgccgg tagaagtgta ccggtctcag cacctcggtc acgccgggta
     3181 tcagattggg ggcgaccatg agcgcgccga gcaccgacac ctcggcatcg aggttctgcg
     3241 gcgggacgta gtccgtcttg ctcccggcgc gaccgggtcc gttgtcaccc acgaatcgct
     3301 ccccgcatcc gcggcatcag atcaccaggg cggtcgaggc cgctacttct tctcctcggg
     3361 cacgactatg accttgactt cggccggttc gaccgaggcg tgcacgtcta ccttgaccat
     3421 gtaggtgccg acggccttga tcgcgtcttc gaggtgcacg ttgcgcttgt cgatgcgcac
     3481 tttgcgcgcc tggaagatgg ccgtcgctat gtcttgactg gttatcgaac cgaaaagccg
     3541 atcgccgctg ccgacgggcg cggggatcgt gagcaccgtc ttgctcaaca tgtcgcggga
     3601 atcctccgcc tgcgcggctt cacggcgctc gcgggcatca cgctcttctt gcgccgtccg
     3661 caccgcggct atgcggctgg ccgtggcgac ctctgccagt ttctgcggca gcaggaaatt
     3721 ccgggcgtag ccgtcggaca cgttcgccac gccgcccttc ctgccgacct tgtcgacgtc
     3781 ttgccgcaag atgacttcca tctaactctc ctcctggtcg cccgtgcccc gggagcccgc
     3841 agggccggca gaaccggcct tcggcgctcg aggcgcgaag cgcttcctat agtcgaacca
     3901 cgtatccaac aatccggccc agcttgtgat ctgaagtaga agctggccaa gaaccgcgcc
     3961 cccgtacacg gcgatgcgca ctccggcgcc gtgtcgtcgg gtcaccacga accagtgcgc
     4021 caccgatagt ccctggatga agaacagcgt ctcgaagaac ataaagaagt tcaaccctat
     4081 cagacgcgcc gcctccgccc acgctccgag agccgggccc agagccatca gacccagtcc
     4141 cacgatgaaa ccgtacgacg ccgaccagtg caatcggaac cgcgagaacg ccagcccgct
     4201 cgccgccttc tctccgaggt gcggaaagac caccgccacc aaggccaagg tggcgctcgc
     4261 catgatgaga cccgccaccc ccaggatcga agggagtagg taggggacga tgtcgagcca
     4321 ctcccggatc tgacgcgacg ccgattcgat caccgccggg tccacaccca tagacgagta
     4381 gagccggctc gcggccgcca gcgaatcgtc caccagcttg acgatctggg tccgatccaa
     4441 ccccgccagg acccacgccg ccaccgtcga gacggcgaac gccacggctc caccgacggt
     4501 cgtcgccaag agagtagccg agaagcccag ccgacgagcg agggcccatc ccacgacgac
     4561 acccaccagc gcgctgagca ggaacgtgag caggccgttg cccagaccgg tgagagctgc
     4621 gctgaggacg cccgcgccga acgcggcagc cacgcccacg accacgcccc ggcggaacgt
     4681 cacgtaggcg gcgggcatcg gcagcaccgg catcaacacg gcgcccaaca ccggcacgta
     4741 ggcgatcaag gcgctcaaca caacagcgag cgccaccgcc ttccctgcct cgctcgtcgc
     4801 tcctgtggag aagctactca tgcctggtcg aggcgcccgc cccaagcggg cagccgagca
     4861 cttctaccgg ttggagtacg gcagcagagc catctcgcgg gcgcgtctga tcgcgaccgc
     4921 gagctgacgc tggtggcgcc ggcaggcccc ggtggtgcga cgcgaacgga tcttggcccg
     4981 ctcggacgtg aaccgccgaa gctggttgaa gtccttgtag tcgacgtgct ggatcttctc
     5041 tttacagaaa tagcagtact tgcgacggcg gttcccggtt gtgggtctcg tgcgccgcct
     5101 cttggcgaat gccacgtgta tgcctcctga tggctgacta gaacggtatg tcgtcctcgt
     5161 cgtcgatctt gctgaagtct atgtcgcgaa agtccgcgtc gccggagttc gagccggatc
     5221 ctggggccga cgcacggttg gaacgtccgc cgcctgcacc gccggaactg ccggaatctc
     5281 cggagccctc gtttgcgccg cccaagaaca tcaccgtgtc ggcgatgatg tccactctgc
     5341 tgcgcttcga tccgtcctgg gcctcccagg agtcgtagtt caagcgaccg tcgacggcaa
     5401 cttggcgacc ctttgcaagg tagcgtgcgc agttctcgcc ctgctgtccc cacacggtga
     5461 cgtcgaagaa gttggccttc tcctgccact gtccggacga atccttgacc gaggagttga
     5521 ccgcgactcc cagcttgcac aaagacgtgc cgctaggaag ctgcttgagt tcagggtctc
     5581 ttgtcaggtt gcccacgagt gtgacgtggg ctattcccct agccattgtc tactcctcgg
     5641 tggtggtcgc cgcgacggcc gcgacggccg cgacgggcgc tacaggatct tcgagacgca
     5701 cgggcatgtt gcggaccacc gcatcggtga ttccaagcac gcgggtgatc tcatcgagag
     5761 tcttgggctc tacgttgaag tacatcacgt agtagtagcc ctcggtcatg tgggcgatct
     5821 cgtaggcgaa gcggcgtttg ccccattcgt cgatcttgcc catcacgcct tggtcggcgg
     5881 cgacgatctc cttgatgcga tcaaggatcg cggcgcgggc ctcgtccacg gtgtcgggac
     5941 gcaggataag catcagctcg tagctgttca gttcggtcac ctccttcgga ctgtgcggcc
     6001 ccggcctctc tgccggagca ggggtgaaac ataggattgt atccggcgca catggcgcac
     6061 gtgccgcgaa acgcggacgg tggagtatac acacggcggc cccaaatgcc cactgctcta
     6121 cccgggaggt cccggtagaa ggccgtcttc ctcgcccgcc gcaacgggcg cgcactcctg
     6181 agcctcgatg ccgaacccgc cgacgtgcag atcgtaggcg acaccatcag cgcacagcag
     6241 acggtctacc tcggccaagc gagcggcgct gacatcgagc gcgatgccac agagcccacc
     6301 gggcacgcct tcgggccgag ggaccacgtc gacctcgagg cccgcctcca ggaggatctc
     6361 ctccgccctc agggccgact gggtgttgtg gaacaggagc accatgcacc cgagtatacc
     6421 gaccccgtcg gacagaagag ccgcgccccg gccggagggc cggggcgcgg ctgctacgag
     6481 cttggcgaga ccttcggtct agccctgggg atcgctcggg ggagtcggcg cagacttcga
     6541 ctcctcgacc gccgcgaccg cggatgcggc ggccggcgcg gcggccggct gtgcaggccc
     6601 cgcatcgtcg tggtgccgct ggtgccctcg gccgccggac gtcctcgcgg tcagcaaccg
     6661 tatgagcctg tatccaagcc aggccaacag gaccagcagg accagaacgg gcagggcacc
     6721 gcccatgaac acgatgatct cgttgacgct gtcgacgaac gcgtgaagag cgtccttgag
     6781 cgagtcgacg aaaccccaac tccccgccgg cttgaactca ctgcccgcct cgtacaagct
     6841 gatggtcagc gtcgagaagc tggtgtgctc gtcgaggaac ctgatgcggc ccttgagttg
     6901 ctcgatctcc atctgggtct gcgagagcac ctgccgcacc tgcaggatct cgtcgatcgt
     6961 cttggccttg tccatgagag ccaggaaagc cttctcctgg acttcggcat tcttcaaccg
     7021 ggcgccgaga tcaagatact cctcggtcac gtcttgggtg tcgacgttgc gcgacttcac
     7081 cgtgcccagc ttggcggcat cgctcagggc ttgggtgaag gcggtctcgg gcacgcgaag
     7141 ggcgatagtg ccactgcgca tggtgctttc cgcgtctgcc gcggtggagt tggacgacac
     7201 cacgtatccg ccgaacttgt ccgccaagag gagcgcctga tcgaacgcta tctggaactg
     7261 tccccgctcg acttcgacca gtagcgtcgc attgctgacg atcttacggt ctagggacat
     7321 ggaagtctcg aagatgccgg cggtgccgct ctcgcccata agcggcgcgc cggatccgga
     7381 cttgttcgag aactggtccc cgctgccggg tagagtgggc gctccggagt ccgatgcgta
     7441 aatggtcgtt gccgggctgc cggcgacgtc acgcgtaggg gagtcccccc cgcacccggc
     7501 gatcagcagg cccagcaaca gtgccaacgc aagcgccgga gcccacctca tgcgaaagcc
     7561 gaactccgcc gggccctgca gtgccgcccg ctgcagccgc gctgcgcggc tcaacgcgtc
     7621 cttcggaaat gaactccccg tgccggacat ctcgccctcc tccatctcgg tcgccgcctc
     7681 gcggggcgac ccctcgtgat cgcgcgcagg ctctgacgag ccgtcaaggc ggctcggttc
     7741 ctcagtcctc cacttcgagc gcgctccgca cctgctggac gatgccgcga acggcctcga
     7801 cgtactcggg ggcacgctcg aacggagggg cctcgtcaag gtcggcccgg cgcacgccgg
     7861 cgctgtaggg aagcgctccc agtatgtgga agccctcgag catcgcctcc atctgagccc
     7921 gctccgacgg atcgaccttg ttgagcacca cgaacagacg cttgatgccg atatcggagg
     7981 cgaggcgcct gatcgaatca gcggtgcgtg cgctgcgggc acccggctcc accactacga
     8041 tcatggcgtc caccccttcg gccgtgcccc gacccaggtg ctcgatgccc gcttccatgt
     8101 cgaggatgac cacctccttg cgccgcagca gcaggtgccg gatgagcgag cgaagaagca
     8161 ccgattccgg acacacgcaa ccgccggctc cctccttgac cgtccccatg accatcagcc
     8221 gtatgccgtc catgggcacg ctcagctcgt cggggatgtc gtccacgcga gggttgatct
     8281 tgaagaagcc gcccatggtg ccgggtgtcg acccggtgcg ctcggcgatg agctccttca
     8341 tgtcggcgat gggtacgatg gcgcgggcca cgtcggcgtc tatgccgagc gcgctcgcca
     8401 ggttggcgtc gggatccgcg tccacggcca gcaccttgta cccatcggat gcgaatatgc
     8461 gcgccatcgt gccggccagc gtggtcttgc ctaccccgcc cttcccagtt atggcgatct
     8521 tcatcgtgaa cgacctttca tcccaaggat gtgcacaacc agagcggaca gcaaaccggc
     8581 gagtccgatg accaatgaca ggcctccgcc cgcgtagagc agggtagcga cgaccaccgc
     8641 gacctggccc ggcgagtcgg cggagcccgg ttctcccgca gacgccgcag cgagcacgga
     8701 cgcgctgatc agcatcacca ccccggcgaa caaaagccgc gcgggagcac gcaccggcga
     8761 gcgctccagc ctctcgttgg cccggccggc cagcctgctc ccgactgagt ctaagcctcc
     8821 gatgaggtca aggcgtgggc cgtgggcctt gcgcgccact ctagagctcc gaaagaccca
     8881 cgccgcgcac ccgccactcc ggccacatcg tccgacctac tccttggttc cgcgcagcgg
     8941 gcgggcgggc acgccgccga ccctggcgcc cgcgccgaca tccgcgttca cgaggctgtg
     9001 agccgacacg accgcaccgt cgccgatgac caccccggcc aagatcgtgc agttcgcccc
     9061 gatgacgaca tcccggccga tgaccaccgg ccccgtgcgc aactcctcac gcaggaactc
     9121 gtgagccaag atgacggtgt tgtagccgat gatggtgttc gcccccacgg atatcaattg
     9181 ggggtagaag acgtcgaagg tcgatccgag tccgggagcg gcgaaggggc cgatgtggat
     9241 cccaatggct cggaggagca tcagcttcca cctcaagaag ggaagatacc tgcaggcgta
     9301 caccacggcg aagttgaccg ccacccgtct cagaggtacc cgactgcgcc agtcccacat
     9361 ggagttcatg cggcccgcgg ggtgactggt gagccgcgcg tgcctcttga tcgcgatgtg
     9421 ttgggtgttg tcttccagca ctgctcacgc ccttcgcggt ccgtttgact gcacgccagt
     9481 ctagcatgag caaccgctaa accccggtgg cccgagtgcc gataggcgat caatggcact
     9541 caattccgca tacacccaca ccgtcgctcc tccttacgag ccggaacatg ctcgaccgtt
     9601 ccaggccaac ctggcccgag gcgtcgccct ttggagcacg ttggccgggg cgttggtggt
     9661 cactctcttg gcggccgggt ggctcgtcgg actccgagag atcgacaccg ctccgccggc
     9721 catagccctc gggttcttct tcctcgcgct ggcccctatc ggtcggtaca tagtcaagaa
     9781 cgaggactcg tggagccact cgcgggacat gaaggtcgcg ggtctcctgg tgacgctcga
     9841 tatcggcacg ggcgctttcg tgctcttctg gacgggcggc cccgaatcgg tgctgatagt
     9901 cctcgtgccg ctcctgcctt tcgccctgcg actactgggc aacatctggt actccgtggc
     9961 ctacacggtc gcgatcgccg cggggttcgc catcatgatc atcggcgaac gactcggctt
    10021 tctgcggcat gccgcttcgc tcttggacgc tgcggtcata tcgcacggcc aggcgacagg
    10081 tctcctcgtg ttcaccacga ctgcgacggt cgtctcagcc accatcctgg ccgacgcgat
    10141 cgccaagatc gtcacgcgaa gggagaccga ggcgatcgcc ttctcggaga agatgaacac
    10201 ccgcgcagag gaactcgccc tgctgctgcg cattggcagg gtgctttctc gctcggccga
    10261 cttcgaagtg gccgcgcgcg aggccctcga ccacgtccat gcccatttcc ggcccgccgc
    10321 cacggtcctc tacctcaagg acccgcacac ggggttgctc gccgtgctga gttcgctggg
    10381 tgagtgggcg ggagacgagc gttccgagac gaccctcgca tcgaccgcat tggacgcccg
    10441 tgaagcgcac atgtaccctg aggccgcagc gggcggacga agccgccact ccaccatgat
    10501 cgcacccctg ctggtggaag ataccggcta cggagccatc aaggtggtcg gccagccggg
    10561 ccgaaccttc gatcgctcca agctggcact cctcgaaact atcgcatcgg agctggccgc
    10621 cacggtaagg agtgccagca actaccagtc ggccaacgca gaactgacgc gcgcgggtgc
    10681 cgaattggcg gcgttgaaca cgttcacgcg ttccgtcagc tcgagcctag acctcgacaa
    10741 catctgccac aacctcctct ccaccgctat gcgattgacc ggcgccgata gcggtctggt
    10801 gacgatcctc tcctcggatg ggaacgcgga tatctcgata ttccgtaact acgaacccac
    10861 cacggagcgc ctcctgcgct ccacagattg gcgccggtct cgcggcctct tcggtcgggc
    10921 tctccgcacc ggcagaccgg tcgtggtcgg cgacgtgagc ctggacagtg aatacgagag
    10981 catcgtgccc gcggtgcgct ccaaggtgtg cgtgccgatc gtcgtccaag gagatgtcgc
    11041 aggcatggtc agcctcgaga gccgcgccac ggacgcctac tcacccaacg acgtggactt
    11101 cctcgccgcc atggcggaat ctgcggccgt ggcggtcaaa aacgcgcgcc tctaccgcac
    11161 cttggaggag tccgccatcc gggacggcct caccggactc tacaaccact cctacttcca
    11221 ccag
//
LOCUS       DASRMJ010000270         8407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_26804, whole genome shotgun sequence.
ACCESSION   DASRMJ010000270 DASRMJ010000000
VERSION     DASRMJ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26804"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFD74_07435"
     CDS             complement(<1..186)
                     /locus_tag="VFD74_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HZK49415.1"
                     /translation="MEGAVENHEFLFTSESVSEGHPDKMADQISDAVLDAVLAADPYG
                     RVACETLITNGLVVIAGE"
     gene            complement(278..586)
                     /locus_tag="VFD74_07440"
     CDS             complement(278..586)
                     /locus_tag="VFD74_07440"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49416.1"
                     /translation="MTFGVKTIPVRVKVYTDDYVVTGLIHTKPGGYKERVSDIVNDPA
                     ARFLVITDATFSSAKQASSSAKHCEALVLRVDDIKLLIPFEREPEARPSGEVGEAQSW
                     "
     gene            complement(600..1007)
                     /locus_tag="VFD74_07445"
     CDS             complement(600..1007)
                     /locus_tag="VFD74_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HZK49417.1"
                     /translation="MTERRPRPDDVGRVETVVDETMLAAAVGSGAVLGLSTPTMIALM
                     ERAAVAAIADSLQPGTSSVGTRVDVQHLAPTALGMKVVAEARVVAVDRRRVSFAVFAS
                     DDRGPIGEGTHERAIIDSSRFAEALRQRQNGNE"
     gene            complement(1031..2329)
                     /locus_tag="VFD74_07450"
     CDS             complement(1031..2329)
                     /locus_tag="VFD74_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZK49418.1"
                     /translation="MNQVWNPEYEMMGREELRDLQFKRLQMSLRWAVTNVPLYRDSWQ
                     SMGLKPQDIRSLDDLAKIPFTTKDHFKQCYPYGLFAVPLERVVRVHASTGTMSRPTVV
                     GYTRGDLNTWAELCARVVMAGGARPQDVAQVAFGYGLQTGAFGLHHGLERIGAMVIPA
                     STGNTRRQLTIMRDYRTTVLVCTPSYAMYMAEAAHDFGFDIGELGLRVGLFGAEPWTN
                     RVREELERRLGVTASDNYGLSEVIGPGVSGECVVKEGLHINEDHFIVEVVDPETGKSL
                     PEGTEGELVFTSLTKEAVPVLRYRTGDLASVSSKPCACGRTLARHSKVYARTDDMIIV
                     RGVNVFPSQVEEVLLEVEGVEPHFQIVLDRKGKLDEMVVQIEVDPAFLPDAMRRLVEF
                     ERHVEDRLQEELGVRARVKLVEPKSVPRHTGKARRVVDHR"
     gene            complement(2326..3072)
                     /locus_tag="VFD74_07455"
     CDS             complement(2326..3072)
                     /locus_tag="VFD74_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49419.1"
                     /translation="MALTLKGVRTGYGRIEVLHGVDCVVGAGDIVTMIGANGAGKSTL
                     LKAISGLTPLWGGSIEFDGTRIDGMSVEGIAALGVVQIPEGRQLFGPLTVQENLELGA
                     YARRNRGKVMARKLDEIFALFPRLQERRRQQAGTLSGGEQQMLAVGRALMAEPKVLLL
                     DEPSMGLAPIIVKEIFTILRELNSKGLGMLLVEQDARIALSLADRGLVLERGRVVLED
                     KAAALLENPDVQAIYFGQHSGRGGARTESG"
     gene            complement(3072..3833)
                     /locus_tag="VFD74_07460"
     CDS             complement(3072..3833)
                     /locus_tag="VFD74_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49420.1"
                     /translation="MPILRVESVSKRFGGLEALRDVTFSLYEGQIKALIGPNGAGKTT
                     LFNLVSAIEAPTEGSIVFAGKEITGMTSDRACRVGIGRTFQHSKLFDDMSVLDNVKVG
                     RHAKSNAGLLAAFFTTPRHRREEQAITEASIRALRRVGIEEHAAAQAGSLPLGERHLL
                     EIARALATEPRVLLLDEPAAGLNNEETDRLADSVRRIRDEGITVLLVEHDMGFVMNIS
                     DEVVVLDYGRKIAEGPPLMIQNDEEVIRAYLGADL"
     gene            complement(3833..4873)
                     /locus_tag="VFD74_07465"
     CDS             complement(3833..4873)
                     /locus_tag="VFD74_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZK49421.1"
                     /translation="MNGGRLLTFARRHSAFAMFAVVALGAPIFLNNAYYLSVLAFTAT
                     RLMISLGLNLLMGQAGQISLGHAAFVGIGAYGSAIMTTRWHMDPWLAMALAACIAAFA
                     AVIIGIPTLKLKGHYLALATLGVGEIVFILLVQTKSLTNGTDGLTGIPALAVGGLRFT
                     QYNPRAFHLLVWGVALVMLLISLNLSDSRVGRSLKGLHRSEVAARSLGVDTAFHKVQI
                     FVVSAVFASIAGSFYAYYVQYISPDSFTLTFSVLLVTGVVIGGLGSVWGAVWGTLLVA
                     LLPEYLRQYDDYTNLVFGILVIVIMIFLPTGMIGVPALLGKWRRGIRGGGRPGSGAAM
                     TEGTGAADGGTV"
     gene            complement(4890..5768)
                     /locus_tag="VFD74_07470"
     CDS             complement(4890..5768)
                     /locus_tag="VFD74_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZK49422.1"
                     /translation="MSFDQFLQYIVSGITNGSIYALTALGFTLIYNATDIINFAQGEF
                     VMLGGLTAVSLHRLGLPLPVAFCGAVLIVMLVGIIFELLAIRPLLKASVLAQIIVTIG
                     ASLAFRTFAMIFWGRDSLPLPAFSGETPIKIFGARMTPQSLWVMGLTLVIVVALQLFY
                     RRTLAGKAVRACSVNPEAARLMGISYSRVVLISFAVSAAISAAGGVLITPSSFMGYGS
                     GAMIGLKGFAAAILGGLGSPIGAVLGGLALGVLESLAVGVVSAGYKDAVAFLILLAVL
                     FVRPQGLMGQRPVEKV"
     gene            complement(5849..7090)
                     /locus_tag="VFD74_07475"
     CDS             complement(5849..7090)
                     /locus_tag="VFD74_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK49423.1"
                     /translation="MHRKNVVIVVAVLAALGLLWMVGCGGGETTTTTAAPTTETTAVA
                     TTDTTAPAGPSGDPILIGAVVSATGPASPLGEPERAVLAMMETQINDAGGVLGRPVQI
                     IIEDDQSNPKEAVTATQKLIQQDKVVALIGCTTSGATLAIKPITAEAGIPQLAMAASN
                     AITDEPPMDWIWRTPQKDALAAARALKYVAETLGVTKIAVLHDENAFGASGAAEIAKS
                     APGLGLEIVATESYKTDDTDVTAQLTKIKGANPEALIVWGTNPGPAVAAKNMKQLGME
                     IPYIGSHGIANKAFIDLAGDAAEGVVFPAGKLLAVESITDPAQKAVVSQFVAAFEAAT
                     GGKPNTFAGHAFDASALLVNAINTAQSTDAAAIQSALNATTGFPGTGGIFNYSSTDHD
                     GLSVDDMIMIKIEGGAWVWAK"
     gene            complement(7232..7639)
                     /locus_tag="VFD74_07480"
     CDS             complement(7232..7639)
                     /locus_tag="VFD74_07480"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HZK49424.1"
                     /translation="MAVAQLSLFLENKRGRIAEVLGILASEDVPVFGFSLADVSDYGI
                     VRILAPTAGNARAILESRGLVVVENQVVSVGIEEGISALAQAVALLSAAGIDIEYMYL
                     TTRRSVVLRVEDHVAVEALLTEHGFECLDGGSL"
     gene            7835..8296
                     /locus_tag="VFD74_07485"
     CDS             7835..8296
                     /locus_tag="VFD74_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZK49425.1"
                     /translation="MQVRQARVADVPEIQRLVNGYASQGVMLPRSLHSIYGQLRDHQV
                     MVEGDRILGCVALSIVWEDLAEIRSLAVVADAQGGGIGRALAEKMIAEAQEIGIRRVF
                     VLTFVEDFFVNMGFHRVEKSELPHKIWRECVHCVHFPDCDEVALVLELDRG"
BASE COUNT         1537 a         2778 c         2566 g         1526 t
ORIGIN      
        1 ctctcccgcg atgacgacga gtccgttcgt gatcaaggtc tcgcaggcca cccggccgta
       61 gggatcggcg gcgaggaccg catccaggac cgcgtcagag atctgatcgg ccatcttgtc
      121 gggatggccc tcgctcaccg attcagacgt gaacaggaat tcatggttct ccactgcacc
      181 ctccatggat gactacgacc gccgcacgcg gtctgggttc gcttcgtagg gtccggcgcg
      241 cgcgcttgcg agcgcgtgac ggccggcccc ggcaacctta ccagctttgg gcttcgccga
      301 cttccccgga cggtcgtgcc tcgggctccc tttcgaacgg gatcaacagc ttgatgtcgt
      361 cgacccgcaa caccagcgcc tcacaatgtt tcgccgagga ggacgcctgt ttggccgaac
      421 tgaaggtggc gtcggtgatc accaaaaagc gtgcagccgg gtcgttaacg atgtccgaca
      481 cccgctcctt gtatccaccc ggcttcgtgt ggatcaaccc cgtgaccacg tagtcatccg
      541 tgtacacctt cacccttacc gggatagtct tgactccgaa cgtcacgagg actcccttct
      601 cattcgttgc cgttctgcct ctgtctcaac gcctcggcga accgagagga atcgatgatg
      661 gcgcgttcgt gggtgccttc tccgatcggc ccccggtcgt cggacgcgaa gaccgcgaac
      721 gagacccgcc gccgatccac tgcgaccacc cgggcttcgg ccaccacctt catgccgagc
      781 gcggtgggcg ccaagtgctg aacgtccacc cgcgtgccca ccgatgaggt ccccggctga
      841 aggctgtcgg ctatcgcagc cacagccgcc cgctccatga gggcgatcat cgtgggagtc
      901 gagagtccga gcacagcgcc ggagcccacg gcggccgcaa gcatagtctc gtcgacgacc
      961 gtctcgacgc gacccacatc gtccggtctc ggccgcctct cggtcacgtt cgcgacgact
     1021 cccgcgcccg ctaccggtgg tcgaccacgc gccgggcctt gcccgtgtgg cgcggcaccg
     1081 acttcggctc caccaacttc acccgggccc gcactccgag ctcctcttgg agacgatcct
     1141 cgacgtgccg ctcgaactcg acgaggcggc gcatggcgtc tgggaggaac gccggatcca
     1201 cctcgatctg aaccaccatc tcgtcgagtt tgcccttgcg gtcgagcacg atctggaagt
     1261 gcggttcgac cccttccacc tccaagagca cctcttcgac ctgagatggg aagacattga
     1321 ctccccgcac gatgatcatg tcatcggtgc gcgcgtacac cttgctgtgc cgcgccagcg
     1381 tgcggccgca cgcgcacggc ttggaactca cgcttgcgag atcgccggtg cgatagcgga
     1441 ggacgggcac ggcttccttt gtgagcgagg tgaagaccag ttccccctcc gtgccttcgg
     1501 gcagggattt gcccgtctcg gggtcgacca cttcgacgat gaagtggtct tcgttgatat
     1561 gcagcccttc cttgacgaca cactcacccg acacaccggg gcctatcact tcagagagcc
     1621 cataattgtc ggaggcggtg acccctaggc gccgctcgag ctcttcgcgg accctgttgg
     1681 tccagggttc ggcaccgaag aggccgactc gcagacccag ctctccgata tcgaagccga
     1741 agtcgtgggc ggcttcggcc atgtacatcg catacgatgg cgtgcagacc aggacggtgg
     1801 tcctgtagtc gcgcatgatg gtgagttggc gacgcgtgtt gccggtggaa gcgggtatga
     1861 ccatggcccc gatgcgctcc agcccgtgat gaagcccgaa agctcccgtc tggagtccgt
     1921 atccgaacgc cacttgggct acgtcctgcg ggcgggcgcc accggccatg accactcgag
     1981 cgcagagttc cgcccaggtg ttaaggtctc cccgcgtgta acccaccact gtcggccgac
     2041 tcatggtgcc ggtggaggcg tgcactctga ccacccgctc cagcggcacc gcgaagaggc
     2101 cgtaggggta gcactgcttg aaatggtcct tggtggtgaa ggggatcttg gccaggtcgt
     2161 cgagagaccg gatatcttgc ggcttgagcc ccatggactg ccaggaatcc cggtagagcg
     2221 gcacgttggt gactgcccac cgcagcgaca tctggagtcg cttgaattgc aggtcgcgga
     2281 gttcctcgcg gcccatcatc tcgtattccg ggttccagac ctggttcatc cgctctccgt
     2341 cctcgctccg ccccggccgc tatgttggcc gaagtagatg gcctgtacgt caggattctc
     2401 gaggagcgcg gccgccttgt cctccaagac gaccctcccc cgctccagta cgaggcccct
     2461 gtcggcaaga ctaagcgcaa tgcgcgcgtc ctgctctacg agcagcatcc ccagaccctt
     2521 cgagttgagt tcgcgtagga tcgtgaagat ctccttcacg atgatcggcg cgagtcccat
     2581 cgacggttca tccaacagta acaccttcgg ctccgccatg agagcccgac cgacggcgag
     2641 catctgctgt tcgccgcccg acaacgtccc agcctgctgg cgcctgcgct cctgcagcct
     2701 agggaagagc gcgaagatct cgtcgagctt ccgggccatc accttgcccc tgttgcgccg
     2761 tgcgtaggcg ccaagctcga ggttctcctg aaccgtgagc ggaccgaaca gctgtctccc
     2821 ctcggggatc tgcaccaccc ccagggccgc gatgccctct acgctcatcc catcgatgcg
     2881 cgtgccgtcg aactctatcg agccacccca cagcggggtg aggcccgaga tggccttgag
     2941 taatgtgctc ttgcctgcac cgttggcccc gatcatagtc acgatgtccc cggccccgac
     3001 gacgcagtcc acgccgtgga gcacttcgat ccggccatac cccgtgcgta cccccttcag
     3061 cgtaagggcc attacagatc ggcccccagg taggcgcgga tcacttcttc atcgttctgg
     3121 atcatgagcg gaggtccctc ggcgatcttg cgtccgtagt cgagcaccac gacttcgtcg
     3181 gagatgttca tgacgaagcc catgtcgtgc tccacgagga gcacggtgat cccttcgtcc
     3241 ctgatgcgcc gcacgctgtc cgccagacga tcggtctcct cgttgttgag cccggccgcg
     3301 ggttcgtcca ggagtagcac gcgaggttcc gtggccagcg cgcgggcgat ctcaagaaga
     3361 tggcgctcgc caagaggcaa cgaccccgcc tgcgcggcag catgctcctc gatgcccacc
     3421 cgccggagag cccgtatgga tgcctcagtg atggcctgct cctcccgcct gtggcgcgga
     3481 gtcgtgaaga acgcagcgag gagtccagcg ttgctcttcg cgtgtcggcc caccttcacg
     3541 ttgtccagga cactcatgtc gtcgaagagt ttgctgtgct ggaacgtgcg gccgatgccg
     3601 acacgacagg ccctgtcgga cgtcatgccg gtgatctcct ttccggcgaa tacgatgctg
     3661 ccctcagtgg gtgcttctat cgccgaaacc aggttgaaca gcgtggtctt acccgccccg
     3721 ttcggtccga tcagggcctt gatctggccc tcatacagcg agaaggtcac atcgcgcaac
     3781 gcctccagcc caccgaagcg cttgctcacc gattcgacgc ggaggatggg catcagaccg
     3841 tgcctccatc cgcggcgccc gttccctcgg tcatagccgc gccgctgccc ggacgaccac
     3901 cgcccctgat gccgcgccgc cacttgccca acagtgcggg caccccgatc atcccggtgg
     3961 gcaggaagat catgatcacg atcaccagga tgccgaacac caggttggtg tagtcatcgt
     4021 actggcgcag atactcaggc agaagtgcca cgagcagggt gccccacacg gccccccaca
     4081 cgcttcccaa ccctccgatg accacgccgg tgaccagcag caccgagaac gtgagcgtga
     4141 agctgtcggg gctgatgtac tgcacgtagt aggcgtagaa gctgccggcg atgctcgcga
     4201 acaccgccga gaccacgaat atctgcacct tgtgaaaagc cgtgtccacc ccgagcgacc
     4261 gcgccgccac ctccgaacgg tggagtccct tcaacgaccg acccacgcgg gagtccgata
     4321 gattgagcga gatcagcaac atcacgagcg cgacccccca cacgagcagg tggaatgctc
     4381 gagggttgta ctgggtgaag cggagccctc cgacggcaag agctgggatc ccggtgagac
     4441 cgtcggtgcc gttggtaagg ctcttggtct gcacaaggag gatgaagacg atctccccca
     4501 ctccgagcgt ggctagggcc aggtagtgtc ccttcaactt cagagttggg ataccgatga
     4561 tcactgccgc gaaggcggcg atgcaggcgg cgagggccat ggccaaccac ggatccatgt
     4621 gccacctcgt ggtcatgatg gccgacccgt acgcgccgat ccccacgaaa gcagcgtgtc
     4681 ccagcgagat ctggcctgcc tgccccatca gtaggttcag acccagcgag atcatcagtc
     4741 gcgtggcggt gaaagccaga acggagaggt agtaggcgtt gttgagaaag atgggggccc
     4801 ccagcgccac caccgcgaac atagcgaacg cggagtgccg gcgggcgaat gtgagcagcc
     4861 tgccgccgtt cacgtggtcg cccccgctct catacctttt ccacgggcct ctgacccatg
     4921 agtccttgag gtctaacgaa gagaacagct agaaggatca ggaacgccac ggcgtccttg
     4981 tagccggcgc tgacgactcc aacggccaaa gattccagca cgccgagtgc caggcctccg
     5041 aggacggccc cgatgggact ccccaagccc ccgaggatgg cagccgcgaa ccctttgagt
     5101 cctatcatcg cgcccgaacc gtagcccatg aagctgctgg gagtgatcaa gactcccccg
     5161 gcggccgaga tagccgcgct caccgcgaaa ctgatcagca cgacgcgcga gtaggagata
     5221 cccatcaagc gggccgcctc gggattgacg gagcaggccc tcaccgcctt cccggccagc
     5281 gtccgccgat agaagagttg tagtgccacg acgatcacga gcgtgagacc catcacccac
     5341 aggctctgtg gggtcatgcg ggcgccgaag atctttatcg gcgtctcccc cgagaatgcc
     5401 ggcagcggaa gggagtccct cccccagaag atcatcgcga aggtgcgaaa ggccaaggag
     5461 gcgccgatgg tgacgatgat ctgcgccagc actgatgctt tgagaagagg acggatggcg
     5521 aggagttcaa agatgatgcc gaccagcatg acgatgagca cggccccgca aaaagctacg
     5581 ggaagaggaa gcccaagccg gtgaaggctc acagccgtca acccgccgag catcacgaac
     5641 tcgccctgag cgaagttgat gatatcggtg gcgttgtaga tgagggtgaa acccagcgcg
     5701 gtgagtgcgt agatactacc gttggtgatc ccgctcacga tgtactgcaa gaactgatcg
     5761 aatgacaact ggccccctgg gttccgcggt agaaatgcgg cggcggcctc caacggaggc
     5821 cgccgccgca ttcgacaacg tcagactgct acttcgccca gacccacgcg ccgccttcga
     5881 tcttgatcat gatcatatcg tcgacggaaa ggccatcgtg atcggtcgat gagtagttga
     5941 agataccgcc ggtgcccggg aagcccgtgg tggcgttcag agccgactgg atcgcggccg
     6001 catcggtcga ttgagcggtg ttgatcgcat tcaccagcag cgccgacgcg tcgaaggcgt
     6061 ggccggcgaa cgtgttcggc ttgccgccgg tggcggcctc aaacgcggcc acgaactggc
     6121 ttaccacagc cttctgggcg ggatcggtga tcgactcgac agcaagcagc tttccggcgg
     6181 ggaagaccac accctcagcc gcgtcgcctg cgaggtcgat gaacgccttg ttggcgatgc
     6241 cgtggctgcc gatgtatggg atctccatgc caagctgctt catgttcttc gcagcgacgg
     6301 ccggtcccgg gttggttccc cacacgatca gggcttccgg gttcgcgccc ttgatcttgg
     6361 tcagctgggc cgtgacatcg gtgtcgtcgg tcttgtagga ctccgtcgcg acgatctcaa
     6421 ggcccaatcc gggagccgat ttcgcgatct cagctgcgcc ggaggctcca aaggcattct
     6481 cgtcgtgcag gacggcgatc ttcgtcacac cgagtgtctc ggccacgtac ttcagggcgc
     6541 gggccgccgc aagggcgtcc ttctggggtg tacgccagat ccagtccatc gggggctcgt
     6601 cagtgatcgc gttcgatgcg gccatagcga gctgaggaat gccggcttca gcggtgatcg
     6661 gcttgatcgc cagggtcgcg ccggacgtgg tgcacccgat cagagcgacg accttgtcct
     6721 gctggatcaa cttctgagta gcggtgaccg cttccttggg gttgctctga tcgtcttcga
     6781 tgatgatctg gaccggacga ccgagcacgc cgccggcgtc gttgatctgc gtctccatca
     6841 tcgcgagcac cgcgcgttcg ggctcaccca gagggcttgc agggcccgtg gcggagacca
     6901 cggctccgat caagatcgga tctccggaag gaccggccgg cgcggtggtg tcggtggtgg
     6961 ctacagccgt cgtttccgtc gtcggggcgg cggtggtcgt ggtggtctct ccccctccgc
     7021 agccgaccat ccaaagcagc ccaagtgccg cgagtactgc tacaacaatg acaacattct
     7081 tcctgtgcat ctactccccc ttagttctcg ttgccgctac gttcctgcta cctacctgcc
     7141 ggcggtccag cctcacgccc gccgacaaca cgcacaacac tctcatcata accagtctga
     7201 gaaatcccta caacattcac actgaccgag actacagcga acctccgtcc aggcactcga
     7261 acccgtgctc ggtcagcaga gcctcaaccg ccacgtgatc ctcgacgcgc agcaccacag
     7321 atcgccgcgt ggtgaggtac atgtactcga tgtcgatccc tgcggccgat agcagtgcga
     7381 cagcctgtgc cagcgccgag atgccctctt cgatcccgac ggacaccacc tggttctcca
     7441 ccacgaccaa cccacgactc tcgaggatcg cccgcgcgtt gccggcggtg ggcgcaagaa
     7501 tgcggacgat gccgtagtcg ctcacgtcgg ccagggagaa tccgaagaca gggacatcct
     7561 cggaggcaag gatcccgagc acctcggcga tacgacccct cttgttctcg agaaagagtg
     7621 aaagttgggc tacggccatg acgaccctcc gattccagtc cctgagcgcg tctttgaatg
     7681 cgcgctgcat tctacgacaa caaagtgggc accgcaacgc tctaacccta accttggata
     7741 ggcggcgggc tgcgctgcgg gtcccgcctt gcggtaccat gtgctccgca gctggcgctt
     7801 gatggccccc cagtcctccc agaggagcgg cgtgatgcag gtaagacagg ctcgtgtggc
     7861 cgacgtcccc gagatccaga ggctcgtgaa cgggtacgcc tctcaaggcg tcatgcttcc
     7921 gcgctcgctg cattcgatct acgggcagct gcgcgaccat caggtcatgg tcgaaggcga
     7981 ccggatcctt ggatgcgtgg ctctctccat cgtgtgggag gatctggctg agatcaggtc
     8041 attggcggtg gtagccgacg cgcagggtgg cggcataggg cgcgcgctcg cggagaagat
     8101 gatcgccgag gctcaggaga tcggtatccg ccgagtgttc gtgcttacct tcgtcgagga
     8161 tttcttcgtg aacatggggt tccatcgggt ggagaagtcg gagctccctc acaagatctg
     8221 gcgcgagtgt gtccactgcg tgcactttcc cgattgcgac gaggtagccc tcgtcctgga
     8281 gctcgatcgg ggctgatcgg acggatcgcg tcgccggcgt ggacgacgcg gctcacggtc
     8341 gccgctatcc ggggcgaagc gccgcttcta cgtatttcac cgatggatcc cagaggatcc
     8401 actccat
//
LOCUS       DASRMJ010000271         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_310777, whole genome shotgun sequence.
ACCESSION   DASRMJ010000271 DASRMJ010000000
VERSION     DASRMJ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310777"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VFD74_07490"
     CDS             <1..1050
                     /locus_tag="VFD74_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK49426.1"
                     /translation="QLPGLVETVEAKSSTDMFFHLSMFTPEQLDEIPQYIEDFGVTSF
                     KFYMCGVTGVFPNVEDDFIADGLKRLVGLGGHLTGCVHCENQEMFNADFAKVAEATLD
                     GGLREWAGVGTAESEENAVLRACRLAGDTGARFYLVHMSSGKGVKAAAANRPAGNLFV
                     ETTSPYLSLDKDDPSGLLAKMLPPIKDASDREALWEAVKDGTINSIGTDNVSLNREIK
                     GADKGMLNAMPGYAVLQTHLPVLLHEGVNKRGLSLERLVELTSKGPAEIYGLYPKKGT
                     IGVGSDADLVIVDLEKEQVVDEGKLYSFSDFSIYNGRTLKGWPVMTVKAGKVVVEDGE
                     VVSDAGIGNYLRRTK"
     gene            1136..1687
                     /locus_tag="VFD74_07495"
     CDS             1136..1687
                     /locus_tag="VFD74_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZK49427.1"
                     /translation="MLQLRIQGRGGQGAQMAGEVLATAFFQEGKYVQAYSTYGGARRG
                     TPVSTFIRIDDDPVRERCDIEEPDAIVCFDPSLLSPALLSGATPQTVLLVNSTRGPEE
                     FADLGDLRVATIDALAVAQGNGLGRIVNSTLLGAFARVLGAPDIEVLLEVVRQVSPRK
                     TEENVKSARDGYDSVRMLKGGAA"
     gene            1684..>2573
                     /locus_tag="VFD74_07500"
     CDS             1684..>2573
                     /locus_tag="VFD74_07500"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK49428.1"
                     /translation="MSAISSGTSGKPGSATHRPKEVTGPPAAARAAAAAGEVPTIWTT
                     GWTDVFNTGTWRAATPVHEWRPSPCHVDCPIGNAIPRWIKPIAEGAHEEAWLALVDTN
                     PFPAVTGRVCHHPCEGHCNREVLEAVVGINGLEHFLGDRALEEGWALPAPGDPQGKSV
                     AVVGGGPAGLSCAYHLRRLGYEVTVLEARPALGGLLRYGIPEYRLPAPVVDAEIRRIL
                     DLGVEVRTNTPVADAAALERLRREFDAVFVAVGAQQAKTLAHLEQGAGAGRVLDGLEY
                     LRRCIEGVARDLGERMTVVGG"
BASE COUNT          435 a          845 c          889 g          404 t
ORIGIN      
        1 caactcccgg ggctcgtcga gacggtcgag gcgaagtcgt ccaccgacat gttcttccac
       61 ctcagcatgt tcaccccgga gcagttggac gagatcccac agtacatcga ggacttcggg
      121 gtcacctcgt tcaagttcta catgtgcggc gtgacgggcg tgttcccgaa cgtcgaagac
      181 gacttcatcg ccgacggcct caagcgcctg gtcggcttgg gcgggcacct caccggctgc
      241 gtccactgtg agaaccaaga gatgttcaac gccgacttcg ccaaggtggc cgaggccacg
      301 ctcgacggcg ggctgcgtga gtgggccggt gtgggcacag ctgagtccga ggagaacgcg
      361 gtgcttcgtg cctgccgctt ggcaggggac acaggagccc gcttctacct ggtgcacatg
      421 tcttcgggga agggcgtgaa ggccgccgcc gcgaaccggc cggcgggcaa cctcttcgtg
      481 gagaccacct cgccgtatct ctcgctcgac aaagacgacc cctccggcct gctcgccaag
      541 atgctgcctc cgatcaagga cgcctccgac cgcgaagcgc tctgggaagc ggtgaaggac
      601 ggcaccatca acagcatcgg caccgacaac gtgtcgttga accgcgagat caagggtgcc
      661 gacaagggca tgctcaacgc catgcccggc tatgcggtgc tgcagacgca tctcccggtg
      721 ctcctgcacg aaggcgtcaa caagcgtggg ctgtcgctgg agcgtctggt ggaactcacg
      781 agcaagggcc cggccgagat ctacggcctc tacccgaaga agggcaccat cggagtcggt
      841 tccgacgcag acctggtgat cgtcgatctc gagaaggaac aggtcgtgga cgagggcaaa
      901 ctctactcct tctcagactt ctcgatctac aacggtcgca ctctcaaggg ttggccggtg
      961 atgacggtaa aggccgggaa ggtggtcgtg gaagacggcg aagtggtgtc ggacgccggc
     1021 atcggcaact acctgcgccg caccaagtag tcggataagc tcggcaacaa cgcccgtcgg
     1081 cagagttcgg cgggcgcggc cgtcttcgga ggccgtcaac ggaggtgttc cagcggtgct
     1141 gcagttgagg atccagggtc ggggaggtca gggagcccag atggcgggcg aggttctggc
     1201 cacggccttc ttccaggagg gcaagtacgt ccaggcctat tcgacgtatg gaggggcgcg
     1261 gcgcggcacg ccggtgtcca ccttcatccg catcgacgac gacccggtgc gggagcggtg
     1321 cgacatcgag gagcctgacg cgatcgtgtg tttcgacccc tccctgctca gcccggccct
     1381 cctctccgga gccacccccc aaacagtgct gctcgtcaat tccacccgtg gccccgagga
     1441 gttcgccgac ctgggagacc tgcgcgtcgc cacgatcgac gctctcgccg tcgcccaagg
     1501 gaacgggttg gggcgcatcg tcaactccac cctgctgggt gcgttcgccc gcgtgctggg
     1561 ggctcccgac atcgaggtgc tccttgaggt cgtgcggcag gtctcgccgc ggaagacgga
     1621 agagaacgtc aagtccgccc gcgacggcta cgactcggtc cgcatgttga aagggggcgc
     1681 ggcatgagcg ccatcagcag cgggaccagc ggaaagcccg gttcggccac gcaccgcccc
     1741 aaagaagtca ccggccctcc ggcggcggcg cgggcggccg cggcggcagg cgaggtgccc
     1801 accatctgga ccaccggctg gacggacgtg ttcaacacgg gcacctggcg cgccgccacc
     1861 ccggtgcacg agtggcgccc gtcgccctgc cacgtcgatt gccccatcgg caacgccatc
     1921 ccccgctgga tcaagcccat cgccgagggc gcgcacgagg aggcctggct ggcactggtg
     1981 gacaccaatc ccttcccggc ggtgaccggc cgcgtctgcc accacccctg cgagggtcac
     2041 tgcaatcgcg aggtgctgga agctgtcgtc ggcatcaacg gactggagca cttcctgggt
     2101 gaccgggctc tggaggaggg ctgggcgctg ccggctcccg gtgaccccca ggggaagagt
     2161 gtggccgtcg tgggaggcgg cccggccggc ctctcttgcg cctatcacct gcgccgcctg
     2221 ggctacgaag tgacggtgtt agaggcccgg cccgcgctgg ggggtctgct gcgctacggc
     2281 atacctgaat atcgtctgcc cgccccagtg gtcgatgctg agatacggcg gatcctcgac
     2341 ctgggcgtag aggtgcgcac caacacgccg gtggctgacg cggctgcgct cgaacgattg
     2401 cgccgggagt tcgacgccgt gttcgtggcg gtgggggccc aacaggccaa gacgcttgcg
     2461 cacctggaac agggcgccgg cgccggccgc gtgctcgacg gactcgagta tctgcgccgc
     2521 tgcatcgagg gggtcgcccg cgatctgggt gagcgcatga cggtcgtcgg cgg
//
LOCUS       DASRMJ010000272         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_424569, whole genome shotgun sequence.
ACCESSION   DASRMJ010000272 DASRMJ010000000
VERSION     DASRMJ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424569"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /gene="radA"
                     /locus_tag="VFD74_07505"
     CDS             complement(<1..1096)
                     /gene="radA"
                     /locus_tag="VFD74_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZK49429.1"
                     /translation="MARTRSLFTCTGCGYQSPRWLGRCPDCGEWNTLVEEVVHARPSN
                     GGGHASAPTPLRLDSVSTDAEERIFTGIDEFDRVLGGGLVPGSLVLVGGEPGIGKSTL
                     LLQALLSLQGRGIPTLLVSGEESAAQVKMRARRLEGPFDGLRLISETRTGSVVACLDE
                     HRPAVCVVDSVQTLWSEDISSAPGSVSQIRDVTGQLLRVAKEKGIIMLLVGHVTKSGD
                     LAGPRVLEHMVDAVLSFEGDRSRPYRLLRGVKNRFGSTNEVGVFQMTGRGLLGVPDPS
                     SLFLEEGSDSPGSAVLAAMEGTRCLLAEVQALVVPSGLAMPRRVTRGVDGNRLAMVTA
                     VLTRRAGLHLGECDIFVNVAGGLSVEDPGAD"
     gene            complement(1108..>2985)
                     /locus_tag="VFD74_07510"
     CDS             complement(1108..>2985)
                     /locus_tag="VFD74_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HZK49430.1"
                     /translation="RRTKNNPVLIGEPGVGKTAVVEGLARRIAANEVPELLKDKQIYT
                     LDLGALVAGSKYRGEFEERLKKVMKEIGERGDIILFIDEIHNLVGAGAAEGAIDAASI
                     LKPALARGEIQTIGATTIDEYRKYLARDAALERRFQQITVKEPSVEETQEILSGLRDR
                     YEAHHRITITDEALEAAAYLADRYISDRFLPDKAIDLMDEAAARKRIKSMTAPPDYKE
                     LEDKIQAVRKEKEAAIEGQEFEKAANLRDRERRLINEKKELEERWKSSDEGSRASIGE
                     EEIAEVVAMWTGIPVVKLTEAETQKLLRMEDELHKRIVGQDEALTAVSKAIRRSRAGL
                     KDPKRPGGSFVFLGPSGVGKTELARTLAEFLFGDEDALIQVDMSEYMEKHAVSRLVGS
                     PPGYVGYDEGGQLTEAVRRKPYSVVLLDEIEKAHPDVFNILLQILEDGRLTDAQGRTV
                     DFRNTIVIMTSNVGAGIISKGQTLGFGESLGDGGLEYGDMKSRVMGELKKIFRPEFLN
                     RIDEVIVFHKLSKDQIYDIIDLMLARLRVQLTDRGINLELTNEGKELLLEVGYDPAMG
                     ARPLRRAIQRHIEDVLAEEVLSGKLPPGSTVVMGRDGEHLVIVDVLTGASEVLVGEES
                     Q"
BASE COUNT          491 a         1041 c          891 g          562 t
ORIGIN      
        1 ggtcggcgcc cggatcctcc accgagaggc ccccagccac gttcacaaag atgtcacact
       61 cgcccaagtg cagccctgcc cttcgggtga gcaccgccgt caccatggct agtcggttgc
      121 cgtccacgcc ccgcgtaacc cgacgaggca tcgcgagccc cgagggcacc accagcgcct
      181 gcacctcggc cagcaggcat cgtgtgcctt ccatcgccgc cagcaccgcc gaccccggac
      241 tgtccgatcc ctcttcgaga aagagcgatg aagggtcggg gacgccgagc agtccgcgtc
      301 cggtcatctg gaagacgccc acctcgttgg tggaaccgaa ccggttcttg acgccccgaa
      361 ggaggcgata gggtcggctg cgatctccct cgaacgacag gacggcgtcg accatgtgct
      421 cgagcacgcg cggtccggcg aggtcgccgg acttcgtgac gtgacctacc agcaacatga
      481 tgatgccctt ctctttcgcc accctcagta gttgtcctgt cacatccctg atctgcgaca
      541 cggaccccgg agccgaggag atgtcctcgg accagagggt ctgcacgctg tccacgacgc
      601 acaccgcggg gcggtgctcg tcgagacagg ccaccaccga ccccgtgcgg gtctcgctga
      661 tcaggcgcag accgtcgaac gggccttcga gcctgcgggc acgcatcttc acctgggcag
      721 ccgactcctc gcccgacacc agcagtgtcg ggatgccccg cccctgcagg ctcaggagcg
      781 cctggaggag cagagtgctc ttgccgatac ccggctcgcc cccgaccagc accagcgacc
      841 caggcaccag accgcctccc aagacgcggt cgaactcgtc gataccggtg aagatgcgtt
      901 cctccgcatc ggtgctcacc gagtcgaggc ggagcggcgt cggtgccgaa gcatggcctc
      961 cgccgttcga cgggcgcgca tgcaccacct cttccaccag cgtgttccac tcaccacagt
     1021 ccggacaccg gccgagccag cgcgggctct gatagccaca gccggtgcac gtgaagaggc
     1081 tccgggtgcg tgccactctc cgctgcccta ctgcgattct tcgcccacca ggacctcgga
     1141 ggccccggtg aggacatcga cgatgaccaa gtgctccccg tcacgcccca tgacgaccgt
     1201 cgaacccggc ggaagcttcc cgctgaggac ctcctcggcg aggacgtctt cgatgtggcg
     1261 ttggatggcc cggcgcagcg gcctggcccc catcgccgga tcgtagccga cttccaggag
     1321 aagctccttg ccttcgttcg tgagctccag gttgatgccc cggtcggtaa gttgaacgcg
     1381 cagccgagcc aacatgaggt cgatgatgtc gtagatctgg tccttgctga gcttgtggaa
     1441 cacgatgacc tcgtcgatcc ggttcaggaa ctccggacgg aagatcttct tcaactcgcc
     1501 catcactcgg ctcttcatgt cgccatattc caggccaccg tcgcccaagg actcgccgaa
     1561 cccgagggtc tggcccttgg agatgatgcc ggccccgacg ttcgaggtca tgatcacgat
     1621 ggtgttgcgg aagtcgaccg tgcgcccctg ggcatcggtg agacggccgt cctccaagat
     1681 ctgcaggagg atgttgaata catcggggtg cgccttctcg atctcatcga gcagcaccac
     1741 actgtagggt ttgcgccgca ccgcctccgt gagctgaccg ccttcgtcgt agcccacgta
     1801 gccgggagga gagccgacca gccgactcac cgcgtgcttc tccatgtatt cggacatatc
     1861 gacctggatg agcgcgtcct cgtcgccaaa gaggaactcc gccagcgtgc gggccaattc
     1921 cgtcttgccc actccggacg gacccaagaa cacgaacgat ccaccgggcc gcttcgggtc
     1981 cttgagaccg gcgcgcgagc gcctgatggc cttcgatacc gccgtcaagg cttcgtcctg
     2041 gcccacgatg cgcttgtgca gctcgtcttc catgcgaagc agcttctgcg tttcagcctc
     2101 ggtgagcttg accaccggga tgccggtcca catcgccaca acctcggcga tctcttcctc
     2161 cccgatagag gcgcgggagc cttcgtcgga actcttccag cgctcctcga gttccttctt
     2221 ctcgttgatc aagcggcgct cgcggtcgcg caggttggcg gccttctcga actcctgccc
     2281 ttcgatagcc gcctctttct ctttgcgcac ggcctggatc ttgtcctcga gctccttgta
     2341 gtcgggcggg gcggtcatgc tcttgatccg cttgcgtgcg gccgcctcat ccatcaaatc
     2401 gatagccttg tcgggcaaga aacggtcgct gatgtagcgg tcggccagat aggccgcggc
     2461 ctcgagcgcc tcgtcggtga tcgtgatgcg atggtgcgcc tcgtaccggt cgcgcaggcc
     2521 gctcaagatc tcctgggtct cttccacgga gggttctttc accgtgatct gctggaagcg
     2581 cctctccagc gcagcgtcgc gcgccaggta cttgcgatac tcgtcgatag tcgtggcgcc
     2641 gatcgtttgt atctcaccgc gggcgagcgc cggcttcaag atggacgccg cgtcgatggc
     2701 tccctcggcc gcaccggctc ccacgagatt gtggatctcg tcgatgaaca ggatgatgtc
     2761 accccgctcg ccgatctcct tcatgacctt cttcaagcgt tcctcgaact cgccgcgata
     2821 cttcgagccg gccaccagag cacccaggtc gagcgtgtag atctgcttgt ctttgaggag
     2881 ttcgggcact tcgttggcgg cgatgcgccg tgcgaggcct tcgacgaccg cggtcttgcc
     2941 tacacccggc tcgccgatca gcacagggtt gttcttggtg cgacg
//
LOCUS       DASRMJ010000273         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_383372, whole genome shotgun sequence.
ACCESSION   DASRMJ010000273 DASRMJ010000000
VERSION     DASRMJ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383372"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..1396
                     /locus_tag="VFD74_07515"
     CDS             425..1396
                     /locus_tag="VFD74_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZK49431.1"
                     /translation="MTSTPARWRVVLAFAAVYIIWGTTYLAIRFAVETIPPFLMGGVR
                     FVLAGALLYSWTRLRGVSRPDPAQWRPAFLSGVFLIFLAQGSVVWAEQYIASGLAAVL
                     VGMVPIWMMALNWMRPGGQRPDGVLLAGVFTGFVGVIVLLAPWQAGTRDVPLLAALAV
                     IFGSICWTAGSLYTRTLRLSTSHFQATAMQMLCGGAMLVVLGTVTGEWSGLSLGAVSA
                     RSAVSFFYLAIFGSIVALTAFTWLMSVWPPSQVSTYAYVNPVVAVALGWVLAEERLSL
                     RMGIAVAVIVGSVVLVTMGKVSDRVQRVAHQPAADPGLALFEAADHV"
     gene            1454..2311
                     /locus_tag="VFD74_07520"
     CDS             1454..2311
                     /locus_tag="VFD74_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK49432.1"
                     /translation="MNPAFWGVVSAFGWGTADFIARSTGRAMGPRTALFGMLSVGAVV
                     LTAIVWIGRLPLSLDPSGYGLLLVSGVGTMLGTLLLYRGLARGPLTVVAPIVGAYPAL
                     NVVVNLVRGIHPSPPQWVAMIAVIAGVAVVALAAPQTEGPTESSRRHVRRSALIAVAA
                     AVVFAATMTSFQDAMVIYGELQTVMLARWISLIAVSLLIIGRGDMPRLPAKQAPSLLA
                     QGLLDGGAYLSLLYGSHGEGVGIVVVVSSTFAAVTVFLARILLREAMSLPQWGAIALI
                     VGGVVVLSG"
BASE COUNT          327 a          831 c          830 g          462 t
ORIGIN      
        1 atcttactcg actgcctctt cgtgggatag ccccggagca gtgagccctg catccaccga
       61 gggggagcgg gtctccaccg ccagaggtat ctcgggtcca gtcggcacca acaggcgcgc
      121 cgcgaagatc cccctgccac gggctcggca gaaggagtgc tgccggcaac aggaccgcac
      181 tcgctacgga tgtctgcgag aacatgatga gttcgagcga ggcccgacgc aggaggcgtt
      241 tctggaccgc gatcatgacc ccgatcaccg cagcggtgcc gagcgagagg cccacgccga
      301 gaagtcgcgc gctccgtcca ggcccgggcc gccaccggcc caccaacgac gctaggccgg
      361 ttgacgaact cgatgcggag gactaatttc cccgtctcaa cgaagcgccc acgtaaggat
      421 cgccatgacg tcgacccccg cgcgctggag ggtggtcctt gccttcgccg ccgtgtacat
      481 catctgggga accacctacc ttgccatccg cttcgcggtg gagaccatcc cgccgttcct
      541 catgggcggg gtacggttcg tcctcgccgg agcccttctc tactcgtgga cccgacttcg
      601 cggggtctct cgccccgatc cggcccagtg gaggcccgcg ttcctctccg gcgtcttcct
      661 gatattcctc gctcaggggt cggtggtgtg ggcggagcag tacatcgcgt ccgggcttgc
      721 tgcggtcttg gtgggcatgg tgccgatctg gatgatggcc ctcaattgga tgcgcccggg
      781 cggccaacga ccggacggag tgctcctcgc cggcgtcttc accggcttcg tgggcgtcat
      841 cgtgctgctg gcgccctggc aggcggggac gcgtgacgtg ccattgttgg ccgcactcgc
      901 cgttatcttc ggctcgatat gctggacggc ggggtcgctc tacacccgca cgctgcgact
      961 ctccacctcc cacttccagg ccaccgcgat gcagatgctg tgcggaggag ccatgctcgt
     1021 cgtgctcggg acggtcacgg gcgagtggag cgggctttcg ctcggcgccg tgtcggcgcg
     1081 ctcggcggtc tccttcttct atctggccat attcgggtcg atcgtggccc tcacggcctt
     1141 cacatggctg atgagcgtat ggcctccttc ccaagtctcc acctatgcct acgtgaaccc
     1201 ggtggtggcg gtggccctgg gttgggtcct ggccgaggaa cgcctttcgc tgcggatggg
     1261 cattgctgtc gccgtcatcg tggggtcggt ggtcctggtc accatgggca aggtctcaga
     1321 cagggtgcag agggtcgcgc accagccggc cgccgacccg gggttggcgt tgttcgaagc
     1381 cgccgaccac gtctgaggcc gtgcgcgagc ccaccttcga cgccgcccgg accccccgca
     1441 gcggcttgag gcgatgaatc ccgcattctg gggagtggtg tcggcattcg gttggggcac
     1501 ggcggacttc atcgcccggt cgaccgggcg ggcgatgggc cctcgcaccg ccctgttcgg
     1561 gatgttgagc gtcggagccg ttgtcttgac cgcgatcgtc tggatcggcc gcctgccttt
     1621 gagcttggat ccttccggct atggacttct cctagtgtct ggcgtgggca cgatgctcgg
     1681 cacccttctt ttatatcgag ggctcgcgcg cgggcccctg accgtggtgg ctcccatcgt
     1741 aggcgcctac ccggccctca atgtcgtggt aaacctcgtg cgcggaatcc atccgagccc
     1801 gccccagtgg gtcgcaatga ttgccgtcat cgcgggtgtg gccgtcgtgg ctctggccgc
     1861 gcctcagacg gaaggaccaa cggagtcgag tcggcgacac gtgcgccgga gcgccctgat
     1921 cgcggtggcc gcggcggtcg tcttcgccgc caccatgact tcgttccagg atgccatggt
     1981 gatatacggt gagctgcaga cagtgatgct tgcgcggtgg atcagcctga tcgccgtcag
     2041 cctgctcatc atcggccgcg gagatatgcc ccgcctaccg gcgaaacagg cgccgtcgct
     2101 cctcgcccaa ggactgcttg acggcggggc ctacctctcg ctcctctacg gaagccacgg
     2161 tgagggggtc ggcatcgtcg tggtggtgag ctcgaccttc gcggcggtga ccgtgttcct
     2221 agcccgcatc ctcctgcgcg aagccatgag ccttccacag tggggcgcca tcgccctcat
     2281 cgtcgggggc gtcgtcgtcc tgtcgggcta gaccccgggc cgggtcaccg cgtggtggcg
     2341 acgtcgcgtt gtcgccgacc gccgccccag cgccggcgcg tggcgccgct ggggcggcag
     2401 gcgcgtcagg cctgtgcgcc ggtcagcagc gccggcgcac aggcctgacg
//
LOCUS       DASRMJ010000274         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_383440, whole genome shotgun sequence.
ACCESSION   DASRMJ010000274 DASRMJ010000000
VERSION     DASRMJ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383440"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..2340)
                     /locus_tag="VFD74_07525"
     CDS             complement(40..2340)
                     /locus_tag="VFD74_07525"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HZK49433.1"
                     /translation="MAAFAAMAATAVLTVDRVVQPPMTEALLASVGLGLLVGLPGYLH
                     RRALPGTILLLPLGLYVLLRVVLPLPLDVETRADQLAFYTDELRLGLRAYADDIFPLT
                     LDSAAGFELLLAVGAFLFAAVASFLGLGMRTTVAGIAALVVLLGFTATVDSAPSAGPT
                     LLFLAFATLTLVTSEGARRHGRRLRDGLEGIGVGAAAIGMAVLLLAVFPGLTRPGLAD
                     WKTWDPFNSGRPTVFVFNWKQNYPRLLDPGNAVPIMKVTSRVPSYWRATTLEYFTGDT
                     WLNSAPYPVRLPDGPGPRRIPPIEPEPPGGPMVQKFELRGTSTNYVFTGGSPRSLTLD
                     VSSAVNQSDSGALRTATSLHSPIDYEVTAVVPRVQPELLVGTPRDYPEDVRSLYLDLP
                     MPDAATYRQAREAEARGEETMPWNAWIERPRAVEFEGIYALNEAIVGDAEDPYEATLK
                     IERYLRAHYAYSLEVPPSTYASPIAAFLFDTKMGYCQHFAGAMALLLRLNGIPSRVAV
                     GFTTGEQIGTWTYLVSSNNAHAWVEVYFAGIGWLPFDATPGRSLPLAGPSSASPGFVD
                     PFAGRGAQGEGGGPATPPDATGRIPEGAGAGAGAGTKPWRIPWGIAAPALFAAMLVGW
                     PFTRRRVRERRVRTGRSGDRLRASITLLREDLGVAGVPVRASSTLDQVARLADDTLRL
                     DLAPLVGRAQQVAFGGSYASAEDVARAERMRRGVMRLAWKRRRLACLAAWYGVAPLLD
                     SWRARSANDATGVAQSRKGPTWRLRP"
     gene            complement(2364..>3441)
                     /locus_tag="VFD74_07530"
     CDS             complement(2364..>3441)
                     /locus_tag="VFD74_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZK49434.1"
                     /translation="PPTAGLPVSMSVTVDNRTVAPTTPARLHMDLRAAAGADLYVEIG
                     SLAPRQRLTKTVVIPTLTRGVYSLATPRLETVDPLGLARRRRPVGDDTALTVFPWIAS
                     LDSCVFFGGRGHGQDPRTRASLAHASFDLRGVRPHQPGEPLSRIDWKSTAKTGTLMLR
                     ETEEHTRSAVVLVLDGTEPSQAGGPGEDTFELSVSVLGSIGAYLLREGIAVRLLAHAA
                     HPEELSLEPGERGMRAMLATLARVRPGGDQPLSSSLRAFRSSIAAGISVVVATPSLDR
                     ALVIALTSLVDHGTPTYLLHVDTAAFREEHEFLARDKRMTLLGLQARGVPSLTIHPGD
                     DLTSVLSVAPAEPVAASRRGMFGP"
BASE COUNT          558 a         1095 c         1289 g          499 t
ORIGIN      
        1 gcgtcggctt gctcaatcct cgagtcggat gttgttcgtt cacggtcgaa gcctccacgt
       61 cggtcccttg cgactctggg cgactcctgt cgcatcgttc gccgaacgtg cgcgccatga
      121 gtcgagaaga ggagctacac cgtaccacgc ggccaaacag gccagccgac gcctcttcca
      181 ggccaggcgc atgacgccgc ggcgcatgcg ctccgcccgc gccacatctt cggccgacgc
      241 gtacgaccct ccgaaagcga cctgctgggc acggccgacc aggggtgcca ggtcgagcct
      301 cagggtgtca tcggcgaggc gcgccacctg gtcgagcgtg gaggaggcgc gcaccggcac
      361 cccggctaca cccaagtcct cgcgcagcag ggtgatcgag gcgcggagcc ggtccccgga
      421 gcggcccgtg cggacccgac gctcgcgcac cctgcggcgg gtgaagggcc agcccacgag
      481 catcgcggcg aacagcgcgg gagccgcgat gccccagggg atccgccacg gcttggtgcc
      541 ggcgccagcg ccggcaccgg cgccctcggg gatgcggccg gtcgcgtcgg gaggggtagc
      601 ggggcccccg ccctcgcctt gcgcgccgcg cccggcgaag ggatcgacga agccggggct
      661 ggccgacgat gggcccgcaa gcggtaggct ccgacccggg gtggcgtcga aaggcagcca
      721 cccgatcccg gcgaagtaga cctctaccca ggcgtgggcg ttgttgctgc tcaccagata
      781 cgtccacgtg ccgatctgct ctcccgtggt gaatccgacg gccacccgtg aggggatgcc
      841 gttcagccgc agtagcagtg ccatggcgcc ggcgaaatgc tggcagtagc ccatcttcgt
      901 gtcgaagagg aaggcggcga tcggagacgc atacgtggag ggggggacct ccagtgagta
      961 cgcgtagtgc gctcgcaagt atcgctctat cttcagggtg gcctcgtagg ggtcctcggc
     1021 gtcgcccacg atggcttcat tcagggcgta gatcccctcg aactcaacgg ctcggggcct
     1081 ctcgatccac gcgttccaag gcatggtctc ctcgcctcgc gcttccgcct ccctcgcctg
     1141 ccggtaggtg gcggcgtcgg gcatggggag gtcgaggtac aacgatcgga cgtcttcagg
     1201 atagtccctg ggggtgccca cgagcagctc gggttggact ctgggaacga ccgcggtcac
     1261 ctcgtagtcg atgggtgagt gcaggctggt cgctgtgcgt agggctccgg agtccgactg
     1321 gttgaccgcg ctggacacgt cgagcgtcaa agagcggggc gatccaccgg tgaagacgta
     1381 gttcgtcgag gtgccgcgga gctcgaactt ctgcaccatc ggcccgccgg ggggttctgg
     1441 ttcaatcggc gggatgcggc gaggacccgg cccgtcgggc agcctcacgg ggtagggcgc
     1501 cgagttcagc cacgtatcgc cggtgaagta ctcgagggtg gtggcccgcc aataggaggg
     1561 cacccgggag gtgaccttca tgatcgggac ggcgttgccc ggatcgagaa gtctggggta
     1621 gttctgcttc cagttgaaca cgaacacggt tgggcggccg ctgttgaagg ggtcccacgt
     1681 cttccagtcg gccagtcccg gccgggtgag accggggaag acggccagca gcagcaccgc
     1741 catcccgatg gcggcagcgc ccacgcctat cccttccagc ccatcgcgca ggcgccgacc
     1801 gtgccgtcgc gcgccctcgg aggtgacgag ggtgagcgtt gcgaaggcga gaaagagtag
     1861 tgtgggaccg gcactgggcg cagagtccac cgtggcggtg aatcccagga gtacgaccag
     1921 cgccgcgatc ccggcgacgg tcgtgcgcat gccgagtccg aggaacgaag cgacggccgc
     1981 gaagaggaac gcccccaccg ccaggagcag ctcgaatccc gcggcggaat ccagcgtgag
     2041 cgggaagatg tcgtcggcgt aggcgcgcag gccgagtctc aattcgtctg tgtagaaggc
     2101 gagttgatcg gcacgggtct cgacgtcgag gggaaggggc agcacaaccc gcagcagcac
     2161 gtagagaccg agcggcagga ggaggatcgt gccggggagg gccctcctgt gtaggtagcc
     2221 ggggagcccg accagaaggc ccagacccac ggacgcgagg agcgcctcgg tcatgggagg
     2281 ctggacgacg cggtcgacgg tgagcacggc agtcgccgcc atggccgcga aggcggccat
     2341 gtgagagatc ttccacgcga ctctcatggc ccgaacatgc cgcgccggga ggccgcgacg
     2401 ggctcggctg gggcgacgga gagcaccgag gtcagatcgt ctcccgggtg gatggtcagg
     2461 gagggcacgc cccgcgcctg caatccgagc agggtcatgc gtttgtcccg ggccaggaac
     2521 tcgtgctctt cgcggaaggc ggcggtgtcc acgtggagga ggtaggtggg cgtcccgtgg
     2581 tcgacgagcg aggtgagggc gatcacgagc gcccggtcga gggacggggt ggcgaccacc
     2641 accgagatcc cggcggcgat ggaggagcgg aaggctctga gcgaggatga gagcggttgg
     2701 tctccgccgg gtcgcacccg cgccaaggtg gcgagcatcg cgcgcatccc acgctcgccc
     2761 ggttccaacg acagctcttc agggtgcgcg gcgtgtgcga gcagtctcac cgcgatgccc
     2821 tcgcgcagga ggtatgcgcc gatgctgccg agcacggaca ccgacagttc gaaggtgtcc
     2881 tcccctgggc cgccggcctg cgagggctcc gtgccgtcga ggaccaagac cacggccgaa
     2941 cgtgtgtgct cctctgtctc tcgcagcatg agagtgcctg tcttggccgt gctcttccag
     3001 tcgatgcgac tcagcggctc gcccggctgg tgtgggcgga cgcctctcag atcgaaggag
     3061 gcgtgcgcga gcgaggcgcg ggtgcggggg tcttggccgt gccctcggcc gccgaagaac
     3121 acgcaggaat cgagagaagc gatccaaggg aagacggtga gggcggtgtc gtcgcccacc
     3181 ggtcgcctcc gccgggcgag gcccaaaggg tccaccgtct ccagtctggg tgtggcgagg
     3241 gagtagacgc cgcgtgtcag cgtggggatg actacggtct tggtgaggcg ctgccgcgga
     3301 gccagagagc cgatttccac gtagaggtcg gccccggccg ccgcgcgcag gtccatgtgc
     3361 aggcgcgccg gggtggtagg cgcgacggtc cggttgtcca cggtgacgct catagacacc
     3421 ggcaggcccg cggtcggggg g
//
LOCUS       DASRMJ010000275         9932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_27446, whole genome shotgun sequence.
ACCESSION   DASRMJ010000275 DASRMJ010000000
VERSION     DASRMJ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27446"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1420
                     /gene="nifV"
                     /locus_tag="VFD74_07535"
     CDS             134..1420
                     /gene="nifV"
                     /locus_tag="VFD74_07535"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HZK49435.1"
                     /translation="MRENFGAFLLQRPLGEKPSPVHIDDTTLRDGEQTAGVVFANEEK
                     VRIAKLLDEVGVYQIEAGIPTMGGAEKEAVRQIAAMDLNCSILAWNRAVISDVEASLE
                     CGVDAVAISMSASDIHIEHKLRQSREWVLDSVKRAVLFAKEHNVYVSVNAEDASRADM
                     EFLLAFARTAREAGADRVRFCDTLGILDSFNTFMKVKTLIDVVGIDVEMHTHNDFGMA
                     TANAIAGIKAGATFVNTTVNGLGERAGNAALEEVAMALEYIEGEPLSLRTDKFRELSE
                     YVARASGRPLPTWKPIVGANLFVYECENRANATMSDPANYEVFPPEAVGLTRLYILGK
                     YSGAETLKLKLAEHAIEITDEEAALLTYRVRSKSVAFKRSLFDDELLDIYNSEILGVR
                     EEVGETLLAGEDPIGDAALDAALDAAVAMGTVGDES"
     gene            1410..2633
                     /locus_tag="VFD74_07540"
     CDS             1410..2633
                     /locus_tag="VFD74_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HZK49436.1"
                     /translation="MSPDRVMSKVEKKLSAHKVPVLLVDTTLRDGEQTAGVVFANQEK
                     VRMARLLSDIGVHELEVGSPWLCVEEREAMSEIASLDLNAVTRAFCRSELEDIRLAAE
                     TGVDGVVISISTSDQHIQKKYCRDRAWVLDRLERTAAAAREADLSFVASAEDASRTNM
                     DFLLTYAVAAQGLGAERFRFCDSLSVLDPFMSHRRIRTLVEALEIPVEVHCHNDFGMA
                     TANSLAGIRAGARFVTVSMGGLGERTGNAALEEVVMALKHMDDIDIGVSTARFREISE
                     YVARASSRAIPVWKAIVGTNVFAHESGIHADGVIKNPLTYEVFGPDEVGLTRQLVVGK
                     HSGSRTIQHKFKEFGIDLSDHEANEILSLAREMSVDLKRALFEKELMYVYQDFARKND
                     QRLQDEKIGIMDEDD"
     gene            2693..2896
                     /locus_tag="VFD74_07545"
     CDS             2693..2896
                     /locus_tag="VFD74_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49437.1"
                     /translation="MRSLIVHFAGGTEITIQTAMEPTVVYGALIGDGEWLIIEDSFGE
                     RHYLSVRNVAYLTFSTRKGIGFA"
     gene            2949..3368
                     /locus_tag="VFD74_07550"
     CDS             2949..3368
                     /locus_tag="VFD74_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK49438.1"
                     /translation="MILEFFHNEELDPVALAEFFTRLGWDESERTLKLEWAIASSDDW
                     IVCVLEDELVGFGRTFSLDPRRKVMFDVVVDERFKGYGLAEEIMRRLADTRPAAEFQV
                     FGGGAGARARSGPRVEVDYEVPEASPETYTGRPQAGI"
     gene            3380..4393
                     /locus_tag="VFD74_07555"
     CDS             3380..4393
                     /locus_tag="VFD74_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HZK49439.1"
                     /translation="MVHTVTCIPGDGIGPEIMAATKVAVAATGVQIAWVDVEAGSDVV
                     EKYGTPLPDHVLESLRTTKVGIKGPITTPVGSGFRSVNVALRKELDLFANLRPAVTIP
                     GVQSRYDNIDLVIVRENTEDLYAGVEHMVGKDAAESIKIITRAGSERIARFGFEYARK
                     EGRTKVTAVHKANIMKLSDGLFLQTVRAVAAEYPDIEFEDRIVDNMCMQLVQKPELYD
                     VLVCPNLYGDIVSDLCAGLIGGLGVAPGANFGAEVALFEPVHGSAPKYAGLDKVNPTA
                     LILSAVLLLRHLGERTAADALFQAIKTVIREGRDVTYDLGGSAGTRAMGEAVAAKVEE
                     ITG"
     gene            4656..5762
                     /locus_tag="VFD74_07560"
     CDS             4656..5762
                     /locus_tag="VFD74_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49440.1"
                     /translation="MDDCAIQLEGVAKRFGGFTAVKEMNLGIRRGEFFSLLGPSGCGK
                     TTTLRMIAGFEEPSEGAILLDGVDVARLPPYRRNVNTVFQNYALFPHLTVEKNIAFGL
                     KRKRLPKDEIAKRVVDALRMVELEGRAAYKPDQLSGGMQQRVALARALVNLPSVLLLD
                     EPLGALDLKLRRQMQLELKHIHESVGITFIYVTHDQEEALTMSDRIAVMNMGTVEQVG
                     SPEELYESPKTKFVAGFIGVSNFIEGTLQRDGRGGVLTWAEGERVIVEVPDEGTLTDR
                     VELSVRPEKIRLREAGEEAPAGHCLLRGRVRDVVYLGMTTQYKIDVPSLERQFTVLEQ
                     NVEVASEQTQWVSGEEAQLTWHPSHNRVLPPSGA"
     gene            5785..6936
                     /locus_tag="VFD74_07565"
     CDS             5785..6936
                     /locus_tag="VFD74_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZK49441.1"
                     /translation="MKRLDKELRVMVVDGLGGPMTRRGFIKRAGAAGLLVGASGFLAA
                     CGGGETTTTTAAGGGGGELAKELYFYNWSDYIAEETIPTFQDEFGVKVVYDNYSSNDT
                     LLAKLQSGATGYDIIVPTDYMITTMLGLDLLQEIDLANVPNVANLYQRFREAPFDPGN
                     KYSIPWQWGTTGIGYNSVKVPDFKPSWDMLWDEKYKGKITMMNEIRDVFAVALFRLGY
                     SPNTIDPKEIEEAKQMLIAQKPVLKHYTSDTYIDELASEDSWLSHGWSGDVLQAQMEN
                     EAVLYAVPEEGSLTWVDCMCIPKGAPSKYTAEVFMNYIQQPEVAAGITNYVYYASPNE
                     KAEEFILPEILDDPQIYPPQEVMDKLTFLEELGTDTELYNAAYQELRAS"
     gene            6933..7874
                     /locus_tag="VFD74_07570"
     CDS             6933..7874
                     /locus_tag="VFD74_07570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49442.1"
                     /translation="MTGTPSSGETEARASVILEQHAGPLSRAMARLRGHNFVVANLLM
                     GPTMLWLVLFLILPLGVILVYSFGSRGTYGDVILGFSFDNYLRAFNPTWLPIILRSFG
                     FAAVATILTLLLGYPLAYFIAVYGGRRKNIYLIMVMLPFWTSYLIRTYSWIVILRSQG
                     VINTVLMWTHLTSNPIEILNTPYSVILGLTYGFLPFMTLPLYASLEKLDKSLIEAAWD
                     LGATRFWALMKVTIPLSLPGIIAGTLLTFIPAVGDFITPDLLGGPNTMMIGNLIQQQF
                     LSARDWAYGSALSFSLMAILLLGIFFYVRKAGAENLA"
     gene            7871..8743
                     /locus_tag="VFD74_07575"
     CDS             7871..8743
                     /locus_tag="VFD74_07575"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49443.1"
                     /translation="MSPSTSSSTTTTGAVARRRRPRDQRIKGKGLRAYVAGVYAFLFA
                     PIVVLVLFSFNDSKRNATWKGFTLKWYDALFHDQPVLDSLWLTLRVAIIATIIATVFG
                     SFAAYALTRYKFRGSGLYNGAIYVPLVIPEIVFAVALLSGFNAASVGLSMKTVIISHV
                     AFMVSFVIVVVKARMSGFDNGLEEAAMDLGANEIQTFVYVTLPFMLPGIMAGALLAFV
                     LSFDDFVITFFNSGVGNTTLPLKVYSMVKFGVTPEINAISVFMLLVSILMVLASYKLQ
                     GGPPTVVVKDKIKE"
     gene            8818..>9932
                     /locus_tag="VFD74_07580"
     CDS             8818..>9932
                     /locus_tag="VFD74_07580"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZK49444.1"
                     /translation="MSFDGRLTVADLLSEESLRLQLVAGAPGVRSPISGVHISEMPDP
                     TPWLGKGDLLLTTGLALVEDEDLQVQLVRRLKESGMSGIGVGVDIFMQESSAAMRAEA
                     DAQQLPLFEVPLEIPFKSITGLVFHSLYNADFYQLRRSLSVQDRLLALLLEDRGVDHL
                     VSSVAMLLSTSVVVFDGAGRVVSQAYARAKVTPHLRELMWRCYLTNGCRQTTGVHGQE
                     VGAHEVFYEEVRLGGRVELVLCFVYPKGEAVSEMGRVIAAYVAKLLALEVHRTRDEVL
                     LQERMRAGLLDDILSGSGRDEDLLDRLKRFGFAPGRPLTLVLCDIAGFVSGIDGAAGL
                     EVEESLQRLKILFKEVVDGFFSDRRVPFISLAKSDAVV"
BASE COUNT         1785 a         3108 c         3223 g         1816 t
ORIGIN      
        1 gccgccccct ctacggttcc agcctccggg ggcctcgcgg gcggtcgctc gctcgggccc
       61 acaagccggg gaacggccgg aaaaccgggg tcctatgata gacttcagca tccccggccg
      121 gacaggaggc agtatgcggg agaacttcgg cgcgttcctt ctccagcgcc ccttgggaga
      181 gaaaccgagc cccgttcaca tcgacgacac cacccttcgc gacggcgagc agaccgccgg
      241 cgtcgtcttc gcgaacgaag agaaggtccg tatcgcgaag ctgctcgacg aggtgggtgt
      301 ctaccagatc gaggcgggta tccccaccat gggaggcgcg gagaaagagg ccgtgcggca
      361 gatagccgcc atggacctca actgcagcat cctggcctgg aaccgggcgg tgatctctga
      421 tgtggaggct tcgctggagt gcggcgtcga cgccgtggcc atcagcatgt ccgccagcga
      481 catacacatc gagcacaagc tgcgtcagag ccgcgaatgg gtgctcgaca gcgtgaagcg
      541 cgcggttctg ttcgccaagg agcacaatgt ctatgtctcg gtgaacgccg aggatgcctc
      601 ccgcgccgac atggagttcc tcctggcctt cgcgcgcacg gcgcgggaag cgggcgccga
      661 ccgcgtccgc ttctgcgaca ctctgggcat cctcgactcc ttcaacacct tcatgaaagt
      721 caagaccctc atagacgtgg tggggatcga tgtggagatg cacacgcaca acgacttcgg
      781 catggccacc gccaacgcca tcgccggcat caaggcgggg gccacgttcg tcaacaccac
      841 ggtgaacggc ctcggcgagc gcgcgggcaa cgccgcgctc gaggaggtgg cgatggccct
      901 cgagtacatc gagggagagc cgctgagtct tcgcaccgac aagttccgcg aactgtcgga
      961 gtacgtggcc cgcgcctcag gtcggcccct gcccacgtgg aagccgatcg tcggcgcgaa
     1021 cctcttcgtc tacgagtgcg agaaccgcgc caacgccacc atgtccgatc ccgccaacta
     1081 cgaggtcttc ccgccggaag cggtgggcct cacgcggctc tacatcctgg gcaagtactc
     1141 gggtgccgag accctcaagc tgaaactggc cgaacacgcc atcgagatca ccgatgagga
     1201 ggccgcgctg ctcacctacc gcgtgcgttc caagagcgtg gccttcaagc gctcgctgtt
     1261 cgatgacgaa ctgctcgaca tctacaactc cgagatactg ggagtgcgcg aggaggtcgg
     1321 cgagacgctc ctggcgggcg aggacccgat cggggacgca gccctcgacg cagctctcga
     1381 cgcggcggtg gccatgggga cggtgggcga tgagtcctga tcgagtgatg tccaaggtcg
     1441 agaagaagct gagtgcgcat aaggtgccgg tgctcctcgt cgacaccacg ctccgcgacg
     1501 gtgagcagac cgccggagtc gtgttcgcga accaggagaa ggtgcggatg gcccgcctcc
     1561 tctcggatat cggcgtgcac gagctcgagg tgggcagtcc ctggctctgc gtcgaagagc
     1621 gcgaggccat gagcgagatc gcttccctcg acttgaacgc ggtcacgcgc gcgttctgcc
     1681 ggtcggagct ggaagacatc aggttggcgg cggagaccgg ggtcgacggt gtggtcatct
     1741 ccatcagcac gtccgaccag cacatccaga agaagtactg cagggaccgc gcgtgggtgc
     1801 tcgaccgcct tgaacggaca gccgctgccg cccgcgaagc cgacctgtcg ttcgtcgcct
     1861 ccgccgagga cgcctcccgc accaacatgg acttcctgct gacctacgct gtggccgccc
     1921 aaggcctcgg cgccgagcgt tttcgtttct gcgattctct ctcggtgctc gatccgttca
     1981 tgagtcatcg tcggatccgc actctggtgg aggcgctcga gatccccgtc gaagtgcact
     2041 gccacaacga cttcggcatg gccacggcga acagtctggc cggcatccgc gccggggcgc
     2101 gcttcgtgac ggtctcgatg ggtggcctgg gcgaacgcac cggcaacgcc gctctcgaag
     2161 aggtagtcat ggctctcaag catatggacg acatcgacat cggtgtctcc accgctcggt
     2221 tccgcgaaat ctcggagtat gtggcgcggg cttcgagcag ggctatcccc gtgtggaagg
     2281 cgatcgtggg cacgaacgtc ttcgcgcatg aatcgggcat ccacgccgac ggggtcatca
     2341 agaacccgct cacctacgag gtgttcggcc ccgacgaggt ggggctcacc cggcagctgg
     2401 tcgtgggcaa gcactcgggc agccgcacca tccagcacaa gttcaaggag ttcggcatag
     2461 acctctccga ccacgaggcc aacgagatcc tgtccttggc tcgggagatg tcggtggact
     2521 tgaagcgggc cctctttgag aaggaactca tgtacgtcta ccaggacttc gcccgcaaga
     2581 acgaccaacg cctgcaggac gagaagatcg gcatcatgga tgaggacgac tgacccggat
     2641 ccggtggaac ggatccgagg acggagattc tgagatcacc ttgggaggaa gaatgcgatc
     2701 gttgatagtg cacttcgccg gaggcaccga gatcaccatc cagacggcta tggagcccac
     2761 tgtcgtgtac ggcgctctca tcggcgacgg cgagtggctg atcatcgagg actcgttcgg
     2821 cgagcgtcac tatctgtccg tgcgcaacgt cgcatacctc acgttcagca cgaggaaggg
     2881 catcggcttc gcgtgatgct actccgcttc gacggtgctg cactgcgccc agccggcagc
     2941 atccgggcgt gatactcgag ttcttccaca acgaggagct cgaccccgtc gcgctcgccg
     3001 agttcttcac ccggctcggc tgggacgaga gcgagcggac cttgaaactg gagtgggcca
     3061 tcgcctcctc cgacgactgg atcgtctgtg tgctcgagga cgagttggtg gggttcggcc
     3121 gcaccttcag cctcgacccg cggcgcaagg tcatgttcga cgtggtcgtg gacgagcggt
     3181 tcaaggggta cggcttggcc gaagagatca tgcggcggct ggccgatacc cggccggctg
     3241 cggagttcca ggtgttcggc gggggagcgg gcgcacgcgc gcgttccggt ccgcgggttg
     3301 aagtcgacta tgaagtcccc gaggcgtcgc ccgagaccta cacgggccgc cctcaggcgg
     3361 ggatctaggg aggagcgtag tggttcacac ggtcacctgc atcccgggag acgggatcgg
     3421 tccggagata atggcggcga cgaaggtcgc cgtcgcggcc acgggcgtgc agatcgcctg
     3481 ggtcgatgtc gaggcgggct ccgacgtggt ggagaagtac ggcacgccgc tgccggacca
     3541 cgtgctcgaa tccctgcgca ccaccaaggt ggggatcaag ggtccgataa ccacgccggt
     3601 ggggtccggg ttccgcagtg tgaacgtggc gcttcgcaag gagctcgacc tcttcgccaa
     3661 cctgcgtccg gcggtgacga tcccgggcgt gcagtctcgc tacgacaaca tcgacctggt
     3721 catcgtgcgg gagaacaccg aggatctcta cgccggcgtg gaacacatgg tgggcaagga
     3781 cgccgccgag tcgatcaaga tcatcacgcg cgccgggtcg gagcgcatag cccgcttcgg
     3841 gttcgaatac gcgcgcaaag agggccgcac gaaggtcacc gcggtccaca aggcgaacat
     3901 catgaagctc agcgacgggc tcttcctcca gacggtcagg gcggtggcgg ccgagtaccc
     3961 cgacatcgag ttcgaggacc gcatcgtgga caacatgtgc atgcagttgg tgcagaaacc
     4021 cgagctctac gacgtgctcg tgtgccccaa tctctacggt gacatagtga gcgatctctg
     4081 cgccgggttg atcggcggcc tgggggtggc cccgggtgcc aacttcgggg ccgaggtggc
     4141 gttgttcgag ccggtgcacg gttcggctcc gaagtatgcg gggctggaca aggtgaatcc
     4201 gaccgccctt atcctgtccg cggtgctcct gctgaggcat ctcggagaac ggactgccgc
     4261 cgacgccttg ttccaggcga tcaagaccgt gatccgcgaa gggcgcgacg tcacctacga
     4321 cctcggtggc tcggcgggca cgcgcgccat gggcgaggcg gtggccgcga aggtggagga
     4381 gatcacgggg tagttcgggc ggtcggtcgc gctcggagcg tccgaggcga cctcgcctgc
     4441 tcgttgtggc aatcgacacg ttgggcggcc ggtcttgtgc gttcatccac tcccgcgcta
     4501 ggcgttccgg cggctagcat ggtatagctg tgggggtggt gtgatcgccc gcgtgtcttc
     4561 cgggttgagc gaatcccgag ataggcatat ggttacgcag gcagaggggg tagaagttga
     4621 aggtcgaagg ttcgccggcg caggcggata gctctgtgga tgactgtgcg atccagctcg
     4681 aaggggtcgc aaaacgattc ggtggcttca ccgccgtcaa agagatgaat ctcggcatca
     4741 ggcgaggcga gttcttctcg ctgctcggtc ccagtgggtg cggcaagact acgacgctcc
     4801 gcatgatcgc cggtttcgaa gagccctccg agggagcgat tcttctggac ggggtcgacg
     4861 tggcgcgcct gccgccctac cgccgcaacg tgaacaccgt cttccagaac tacgcgctct
     4921 tcccccatct gacggtcgag aagaacatcg ccttcgggct gaagcgcaag cgcctcccca
     4981 aggatgagat cgcgaagcgc gtcgtggacg cgcttcgcat ggtggagctc gagggcaggg
     5041 cggcctacaa gcccgaccag ctctccggcg gcatgcagca gcgggtggcg ctggcacgcg
     5101 cgctcgtgaa cctgccttca gtgcttctcc tcgatgagcc gctgggcgcg ctcgacctga
     5161 agctccgcag gcagatgcag cttgaactca agcacatcca cgagagcgtg ggcatcacct
     5221 tcatctacgt cacccacgac caagaagagg ccctcaccat gtccgatcgc atcgcggtca
     5281 tgaacatggg tacggtggag caggtggggt cgcccgagga gttgtacgag tcgcccaaga
     5341 cgaagttcgt ggccggtttc atcggagtct cgaacttcat cgaaggcacc ctgcagcgcg
     5401 acggccgggg cggcgtgctc acatgggccg aaggcgagcg cgtcatcgtc gaagtcccag
     5461 acgagggcac tctcaccgac cgcgtggagc tctctgtacg gcccgagaag atcaggctgc
     5521 gcgaggccgg tgaggaggct cctgcgggac attgcctgct ccgggggcgc gtgcgagatg
     5581 tggtctacct gggcatgacc acccagtaca agatcgacgt accttccctc gagcggcagt
     5641 tcaccgtgct cgagcagaac gtggaagtgg cctcagagca gacccaatgg gtctcagggg
     5701 aggaagccca gttgacgtgg catccttcgc acaaccgggt gctccctccg tcgggggcct
     5761 agtccgagaa cgaggaggag gagtatgaaa cgtctggaca aagagcttcg agtcatggtg
     5821 gtggacgggc tggggggacc gatgacccgc cgcggcttca tcaagcgcgc cggggccgcc
     5881 ggcctcctcg tcggcgcctc cggtttcctg gccgcctgcg gcggcggcga gaccacgacc
     5941 accacggcgg ccggcggtgg tggcggtgag ttggccaagg agctctactt ctacaactgg
     6001 tccgactaca tcgcggaaga gaccatcccc actttccagg acgagttcgg cgtcaaggtg
     6061 gtctacgaca actactcgag caacgacacc ttacttgcca agctccagag cggggcgacg
     6121 ggctacgaca tcatcgtgcc cacagactac atgatcacca ccatgctggg actcgacctg
     6181 ctccaagaga tcgatctcgc taacgtcccg aacgtggcga atctctacca acgcttccgc
     6241 gaggcgccct tcgatccggg taacaagtac tcgatcccgt ggcagtgggg caccaccggc
     6301 atcggctata actccgtgaa ggtgcccgac ttcaagccca gctgggacat gctgtgggac
     6361 gagaagtaca aaggcaagat caccatgatg aacgagatcc gcgacgtctt cgccgtggcg
     6421 ctgttccgtc ttggctacag cccgaacaca atcgatccca aagagatcga agaagcgaag
     6481 cagatgctca tagcgcagaa gccggtgctc aagcactaca cctctgatac gtacatcgac
     6541 gagctcgcct ctgaggactc ctggctcagc catggctggt cgggcgacgt gctccaggct
     6601 cagatggaga atgaggcggt tctgtacgcg gtgcccgaag agggctctct tacgtgggtg
     6661 gattgcatgt gcatcccgaa gggggcgccc tccaagtaca cggccgaggt gttcatgaac
     6721 tacatccagc agcccgaagt ggcggcgggg atcaccaact acgtgtacta cgcgagcccc
     6781 aacgagaagg ccgaggagtt catcctgccg gagatcctgg acgatcccca gatctatccc
     6841 ccgcaggagg tcatggacaa gctcaccttc ctcgaggaac tgggcaccga caccgaactc
     6901 tataacgcgg cctaccagga gctgagagcc tcttgaccgg aacgccttcc agcggggaga
     6961 ccgaggcgcg agcctcggtc atcctcgagc agcatgccgg tcccctgagt cgcgccatgg
     7021 cgcgactcag gggacacaac ttcgtggtgg ccaatctgct gatgggtcct acgatgctgt
     7081 ggctcgtcct cttcctcatc ttgcccttgg gtgtgatcct ggtctacagc ttcgggagcc
     7141 gtggcaccta cggcgacgtc atcctgggct tctcgttcga caactacctg cgggcgttca
     7201 atcccacgtg gctgccgatc attctgcgct cgttcggctt cgccgctgtg gcgaccatac
     7261 tcacgctgct tctcggatat ccgctggcct acttcatcgc cgtgtacggc ggccggcgca
     7321 agaacatcta tctcataatg gtgatgctgc cgttctggac cagctacctg atccgcacct
     7381 actcgtggat agtcatcttg cgctcccagg gggtcatcaa cacggtgctc atgtggacgc
     7441 acctgacgag caatccgatc gagatcctga atacgcccta ctccgtcatc ctgggcctca
     7501 cctacggttt ccttccgttc atgacgttgc cgctgtatgc gagcttggag aagctggaca
     7561 agagcctgat cgaagctgct tgggacctcg gtgccacccg attctgggcg ctcatgaagg
     7621 tgaccatccc gctttcgctg cccgggatca tcgcggggac actgctgacg ttcatccccg
     7681 cggtcggcga cttcatcacg cccgacctcc tcgggggtcc caacaccatg atgataggga
     7741 acctgatcca gcagcagttc ctatccgcgc gtgattgggc ctacggctcc gcgctctcgt
     7801 tcagcttgat ggccatcctg ctcctcggca tcttcttcta cgttcgaaaa gcgggcgcgg
     7861 agaacctggc atgagcccgt ccacgtccag cagcaccacg acgaccggtg cggtcgcacg
     7921 gcgcaggagg ccgcgcgacc agcgcatcaa gggcaaggga ctgcgtgcct acgtggcggg
     7981 cgtctatgcc ttcctgttcg ctcccatcgt ggtcctggtg ctcttctcgt tcaacgactc
     8041 aaagcgcaat gccacctgga agggcttcac cctgaagtgg tacgacgcgc tgttccacga
     8101 tcagccggtg ctcgactccc tctggctcac gctgcgggtg gcgatcatcg cgacgatcat
     8161 cgccacggtc ttcggctcct tcgccgccta cgctctcact cgctacaagt tccgcggctc
     8221 ggggctgtac aacggcgcca tctacgtgcc tctggtgatc cccgagatcg tgttcgccgt
     8281 ggcgctgctc agcggcttca atgcggcgag cgtgggtctg agcatgaaga cggtgatcat
     8341 ctcccacgtg gctttcatgg tgtcgttcgt catcgtggtg gtgaaagccc gcatgtcggg
     8401 cttcgacaat gggctcgaag aggctgccat ggacttgggg gccaatgaga tccagacctt
     8461 cgtctacgtg accctgccct tcatgttgcc ggggatcatg gcgggggccc tgctggcttt
     8521 cgtgctctcc ttcgacgact tcgtcatcac cttcttcaac tcgggcgtgg gcaacacgac
     8581 gctcccgctc aaggtctact cgatggtcaa gttcggcgtc acacccgaga tcaacgccat
     8641 ctcggtcttc atgctcctgg tctcgatcct gatggtgctg gcttcctaca aactccaggg
     8701 cggacccccc accgtggtcg tcaaggacaa gatcaaggag tgacctcgcc cggtctccgg
     8761 tagaatgtcc gcgatgcagt tcggacaacg ccgggtctcg gccggggagt gcgctggatg
     8821 tcctttgacg gcaggttgac ggtcgccgat ctcctgagcg aggagagcct ccggctgcag
     8881 ctggtcgcgg gggcgccagg tgtccgttcg cccatctccg gtgtccacat ctccgagatg
     8941 cccgatccta ccccgtggct cggcaagggc gacctcctgc tcaccaccgg tcttgccctg
     9001 gtcgaggatg aagacctgca ggtgcagttg gtgcggcgcc ttaaagaaag cggcatgtca
     9061 gggatcggcg tgggggtaga catcttcatg caagagagct ccgcagccat gcgtgcggag
     9121 gccgacgcgc aacagttgcc actctttgag gtgccgctcg agatcccgtt caagtccatc
     9181 accggtctcg tctttcacag tctctacaat gcggatttct accagcttcg tcgcagtctg
     9241 tcggtgcagg accgtctcct ggccctgctg ctcgaagacc gcggggtcga tcacctggtg
     9301 tcgtctgttg ccatgctgct ctccacgtcg gtcgtcgtgt tcgacggtgc cggcagggtc
     9361 gtgagtcaag cgtatgcccg ggccaaggtg acgcctcacc tgcgcgagct catgtggcgc
     9421 tgctacctga ccaacgggtg tcggcagacc accggcgtgc acggtcagga ggtgggggct
     9481 cacgaggtgt tctacgagga ggtgcgcctc ggcggccggg tggagctcgt tctctgtttc
     9541 gtctacccga agggggaggc ggtgtcggag atggggcggg tgatcgccgc ctacgttgcc
     9601 aagctgttgg cgctcgaagt gcaccgcaca cgggacgagg tcctgctgca agagcgcatg
     9661 agggccgggt tgctcgacga tattctttcg ggatcgggcc gtgacgaaga cctgctcgac
     9721 agactcaaga ggttcggttt cgcgcccgga cgccccctga cccttgtgct gtgcgacatc
     9781 gccgggttcg tctcggggat agacggcgcg gccgggctcg aggtcgagga gtcactccag
     9841 cgtctgaaga tcctgttcaa ggaggtcgtg gacggcttct tctccgatag gagggtgccg
     9901 ttcatctccc tggccaagag cgacgccgtg gt
//
LOCUS       DASRMJ010000276        12621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_468414, whole genome shotgun sequence.
ACCESSION   DASRMJ010000276 DASRMJ010000000
VERSION     DASRMJ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468414"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFD74_07585"
     CDS             <1..1098
                     /locus_tag="VFD74_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZK49445.1"
                     /translation="LSIEEKKGLPTLGELLEGFTKIKLRDKVIFSRQFATMINAGLAL
                     LRALYILEEQTQNPRFRKIISEARQDVEAGMPLSDALERHPAAFDRLYVSMVRAGEVG
                     GVLDLTLERLATQLEKDDSLRRSVKSAMTYPIMIGAFAILVLFALIIFVIPIFGAMYD
                     DLGGQLPLLTRIMVGVSDFMRALWFVVFPALFLAVYGLRRLKNTKTGTETWDKVKLRL
                     PMKLGPIVQKIAVARFSRTLATLVSSGVPILQAIEITGHTSGNTVIEHAMDGVKENIR
                     GGDSIARPLEKIPVFPPMVTQMIAIGEETGALDTMLHKIADFYEDEVDAAIKSLTSIL
                     EPIMMIFIGGIVGLVVISMYLPIFNLFQLVQ"
     gene            1095..1991
                     /locus_tag="VFD74_07590"
     CDS             1095..1991
                     /locus_tag="VFD74_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK49446.1"
                     /translation="MKATAQDAHDRGVGRPAPLHLDVEGARPRLRSLPGREELGARGG
                     PLSGLGESQPPTNEELVERYLNGDVHSLDDLLEVNEGLLHHVLKRFSWATEPYEDLFQ
                     VARLGLMKSAQRFDPQRGHLFSTYAIAIIDGEIRHHLRDSLLIRQPRWARSLYARIQE
                     TQTAFFAEHHRSPTVPELAERVNIREEGVLEIIRVYSGISLHSLDEPLGSPLHDVVDK
                     SLVRALRPQNFSLPIEDRIVLYDALGALSDLQKKIIYLVFFGDLTQQEVADEIGLSQR
                     AVSREQVKALGRLKAILHKRLF"
     gene            complement(2143..2655)
                     /locus_tag="VFD74_07595"
     CDS             complement(2143..2655)
                     /locus_tag="VFD74_07595"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZK49447.1"
                     /translation="MDLKVFIRDVPDFPKPGIMFKDIMPLLQDPNALRYTVDTLAEFS
                     AAKNADVILGAEARGFILGAAVAYSIGAGFVPARKPGKLPWQVTTAEYELEYGFDTLE
                     MHIDAIKPGQRVIIHDDLLATGGTARAKVELVERVGATVVGLAFVIELGFLEGRKKLA
                     GYDVFSLVTY"
     gene            complement(2779..3540)
                     /locus_tag="VFD74_07600"
     CDS             complement(2779..3540)
                     /locus_tag="VFD74_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZK49448.1"
                     /translation="MSGDSGGPAPASRSDPAPGPLDRRGADSHLLTVGQVVQALQGEF
                     PDLTISKVRYLEDRGLVRPERTAGGYRKFDAAGMRALRTVLTLQRDEFLPLEVIKERV
                     DRGTASVVGRALAPSGPFEARRGLLKDESAHTWEEALEVTGVSEAFLRSLGDFHLFER
                     RDPVGDPILSDTDLEVVRICHLLARHGVEPRNLRLLRSSAEREAAMLAQVAAPGLRSA
                     HPERREEGERLLVDLGSLMARLLDLLLYKELIRLA"
     gene            complement(3537..4007)
                     /locus_tag="VFD74_07605"
     CDS             complement(3537..4007)
                     /locus_tag="VFD74_07605"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZK49449.1"
                     /translation="MNDCAKCGTQNQEGANFCCRCGSRLLQRDLSETTVTYAPASSDA
                     AAVLSQTDRTLGRVPTLLIRAGGGREGEQILLDNDLLTLGRNPESHLFLDDVTVSRHH
                     ARIVHDATGFVVEDLNSLNGTYVNRGRVERQHLIDGDEVQIGKFKFAYLEPSES"
     gene            complement(4004..4294)
                     /locus_tag="VFD74_07610"
     CDS             complement(4004..4294)
                     /locus_tag="VFD74_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49450.1"
                     /translation="MGDRIDRIVAGVQALFHDLSVDVREERVTRYVVMELHGGRPFDE
                     LMADPYVVNHTSAEDRLRILESPDILREIEDRIAAEFHDYRRQIAYGDGVAE"
     gene            complement(4307..4585)
                     /locus_tag="VFD74_07615"
     CDS             complement(4307..4585)
                     /locus_tag="VFD74_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49451.1"
                     /translation="MGEHDTSSFELLLHIQSLSDDELKRSAEDLAEEERAVSKTRRII
                     HAKLDILRAEIVRRLRDKHSTGESLFEEGDVEKLSAILSSRGLAEEDV"
     gene            complement(4724..5110)
                     /gene="gcvH"
                     /locus_tag="VFD74_07620"
     CDS             complement(4724..5110)
                     /gene="gcvH"
                     /locus_tag="VFD74_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZK49452.1"
                     /translation="MNPKNLKYHKEHDWARIEGDVATLGVTDYAQESLGDIVYIELPE
                     VGAEVSAGTSYAEIESVKAVSDVFAPLSGTVIEANDEVVDAPELINESPYEDGWLIKI
                     RLSDPSEADDLLSPEDYEEMLAEQEE"
     gene            5369..5863
                     /locus_tag="VFD74_07625"
     CDS             5369..5863
                     /locus_tag="VFD74_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZK49453.1"
                     /translation="MARRRGLVINNRQMRLVDDILEKLRQVSGSRFIALVSTSGQPIT
                     TSTVEGHPDTLSLASLAASSFAATQQLAEVLNEREFTLLFHEGRESNLHVAQVTDQVL
                     LVITFGRETQVGKVRLYTNRAVEVLRPVFDVSEDDDQVSLMIDDDYSLLADEAIDDLF
                     ADME"
     gene            5868..6452
                     /locus_tag="VFD74_07630"
     CDS             5868..6452
                     /locus_tag="VFD74_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HZK49454.1"
                     /translation="MPLINFAAREINYKIVYYGCGLCGKTTNIQYIHKKVNPDARGKL
                     VSIATEEERTLYFDFLPLSLGTVKGMKTRFHLYTVPGQSYYNASRRLVLQGVDGMVFV
                     ADSQITRLDENVESYLNLWDNLLDQGDDLSKLGFVIQLNKRDLSDIFSADDMRELLNH
                     TGSPVLEACALSGIGVFETLKTVCKQVIAKTGQS"
     gene            complement(6517..8208)
                     /locus_tag="VFD74_07635"
     CDS             complement(6517..8208)
                     /locus_tag="VFD74_07635"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZK49455.1"
                     /translation="MIAAVFGFAFVTGDAPGTVPRGISVGGVEVGGLSAEAAAAKVAA
                     SAASLLERPIRVVAGDAVFTVNPAKAGLSIDAQGSVAPGLARTESTSLFREIWGRVFG
                     DSDRLALPFRFEVDDIRLASTLDEVALLVERQSKEARLILEQGRPKAVAAEVGVALDR
                     DGLRAGLIQAMADGRPEVRAPLREIEPAVRTEDVQAVLASVRTGLASPVTLGYQGTLF
                     EVSPGELGAIAAPNPSGIVNGSPLTFDTDAAREVLGERLKSLEDPPVEAEVIPGDDGK
                     SFTVTPSVEGTLIEWPALLSSLARVVLEGGRKYVPIPTATVQPRLTTLDAQELQERRE
                     IASFTTYFSAANSARVNNIRQVATILDGYVIRAGETFSFNAAVGPRTRAAGFDEAPVI
                     RDGALTPGVGGGICQVSTTLFNAVFFAGLPVVERKPHSFYIEHYPVGRDATVSYGATD
                     FRFRNDSDAVLLLSVSTTSQSVTVSLAAPTWDRTISYETSPFNDLVEPRSTEDIPRKL
                     RDPALPSGTVSAVEPGVAGRSVEVRRVVSDPYGVLFEDVFTSVYSPKDFVIRVGG"
     gene            complement(8254..10773)
                     /locus_tag="VFD74_07640"
     CDS             complement(8254..10773)
                     /locus_tag="VFD74_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZK49456.1"
                     /translation="MKAVVMAGGQGTRLRPLTSNMPKPMVPVANKPTAQHILELLSRH
                     GIRDVVMTVAFMPQLIQNFFGDGSSFGMQIDYCVEEVPLGTAGSVKNAQRFLDDTFLV
                     ISGDSLTDFDLTEIVDFHRARDAMVTIALKSVDNPLEFGVVIVDEHGRIERFLEKPGW
                     GQVFSDTINTGIYVLEPEVLDHIPEGVSFDFSHQLFPHLYDSRKPLFGMLCEGYWQDI
                     GSLAQFLQANRDALDGKVQANLPGVRLRGNVWVGHGADFDSLDNIQGPALIGDYARLS
                     GSASIGAHTVLGNNVILRGGASVAGSVVGDGSYLGPGVRVRGAIVGSGVDIRANASIA
                     EGAVLGEGSSVGEKAVVGSQVKVYPGKVIESGASVRSSLIWESRGQSSLFGKEGVRGL
                     INIDITPELVMRLGMAYGTSLSAGSIVTTSRDQHPAARVLNRALTAGLNASGVTVRDL
                     QIAPGSLTRFDLKVGNSAGGVHLRVCADNPEEAEIVLSERPGVPMSPQRERGMENIFY
                     REDYRRVAPSEMGSIVFPARLIETYIGALLTACDIDRIRDRDLRVVLDHGGSGTTVLA
                     SSVLDALRLEVVSVNTRDPREDGADGATRPLVDRGERVGRLVRAMEADVGVLMDPGGE
                     SVTVLDETGAGVPDVSLLLLLLRHACTNYGPGNIVVPLHTTRRAEEVAAECGGNVVRT
                     KVSESALLSEAGKPHSIFAGSGGGRFVLPAFLPAPDALVSFLMVLEMLASTMEHVSRL
                     AGALPEVTVRHAVVECPWSVKGVVMRMLVERLKHQRVSLLDGVKLAVGSADWVQFLPD
                     PGEPVFHVFAEADTLAASDELLARYAHMLQETIDDAGGRTD"
     gene            complement(10790..11320)
                     /locus_tag="VFD74_07645"
     CDS             complement(10790..11320)
                     /locus_tag="VFD74_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZK49457.1"
                     /translation="MLTMFRLLLIPAFIYYLAGDDGGRSVVAGVLFAVASVTDWLDGQ
                     IARRTDTVTDFGRFADPMADRLLIGSALIILLVKHRLPTAPLMVVLVRDTYVMAGYYV
                     LSRRGLRVPVSMLGKAGTAVLMAALFLAVLGIGAAVPILWVGAALSLTSGAHYTLRGF
                     RQFRRPEAGVVTPTGS"
     gene            complement(11419..11492)
                     /locus_tag="VFD74_07650"
     tRNA            complement(11419..11492)
                     /locus_tag="VFD74_07650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11456..11458),aa:Pro,seq:ggg)
     gene            complement(11526..12566)
                     /locus_tag="VFD74_07655"
     CDS             complement(11526..12566)
                     /locus_tag="VFD74_07655"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZK49458.1"
                     /translation="MSESPELAPREGDTLRVAVVGAGSWGTAMARHLALLGLRTRLLG
                     RRMEQAEAICATGMNPSYLRDTQLPPSLDAGVYTEGSLEDVDLVVMAVPSKGYAAVAD
                     SLVDRLPQGVNVLSLTKGVEPGSLRRFSVLLADVWGHLEPRIAVLAGPNHAEEVSLDQ
                     PTATVIASADPSYAESLQLLLSNESFRAYVNHDLVGVELAGAVKNIIALATGMGDGIG
                     YGDNVRAALITRGIAEMARLGVALGAEQLTFAGLAGLGDLVATCTSHHSRNRLAGELI
                     ALGHSPGEVEREMGMVAEGLTAAPAILALARKYGVDMPITRNVVAVVYEQKSVQDCVA
                     DLMSREPRTERH"
BASE COUNT         2399 a         4168 c         3769 g         2285 t
ORIGIN      
        1 ctcagcatcg aggagaagaa ggggctgccc acgctgggag aactcctgga agggttcacg
       61 aagatcaagc tccgcgacaa ggtgatcttc tcacggcagt tcgcgacgat gataaacgcc
      121 ggtctggcgc tgttgcgtgc gctctacatc ctcgaggagc aaacccagaa cccgcggttc
      181 aggaagatca tcagcgaagc acggcaggac gtcgaagcgg gcatgcctct ctctgatgcc
      241 ctggaacgtc accccgctgc tttcgaccgc ctctatgtga gcatggtccg cgctggtgag
      301 gtgggcggag ttctcgacct cacactcgag cgcctggcca cccaacttga gaaggacgac
      361 agcctgcggc gctcggtcaa gtctgccatg acctacccaa ttatgatcgg ggcgttcgct
      421 atcctcgtcc ttttcgcgct gatcatcttc gtcatcccga tcttcggagc gatgtacgac
      481 gacctcggag gtcagctgcc acttctcacg cgcatcatgg tgggagtcag cgacttcatg
      541 cgcgccctct ggttcgtggt gttcccggcc ctgttcctcg ctgtgtacgg cctgaggagg
      601 ctcaagaaca cgaagacggg caccgagacc tgggacaagg tgaagctccg cctcccgatg
      661 aagctcggcc cgatcgtgca gaagatcgca gtcgcgcgct tcagccgcac cctggccacc
      721 ttggtgagct cgggggtacc tatccttcaa gccatagaga tcacgggcca cacctcgggc
      781 aacaccgtca tcgagcacgc gatggatggg gtcaaagaga acatcagggg aggagactcc
      841 atcgcccgcc cgctcgagaa gatccccgtg ttcccgccga tggtcactca gatgatcgcc
      901 atcggcgagg agaccggcgc cctcgacacc atgcttcaca agatcgccga cttctacgag
      961 gacgaggtgg acgctgccat caaatccctg acgtcgatcc ttgagcccat catgatgatc
     1021 ttcatcggcg ggattgtagg attggtcgtc atctccatgt atctcccgat cttcaatctg
     1081 ttccagttgg tccagtgaaa gctaccgcgc aggacgccca cgaccgggga gtcgggcggc
     1141 ctgcgcccct acacctcgac gtcgagggtg cacgacccag gctgagatcg ctgcctggga
     1201 gggaggaact cggcgctcga ggcgggccac ttagcggtct gggcgagtcg caaccgccca
     1261 ccaacgaaga gctggtcgag aggtatctga acggcgatgt tcactcgctc gatgatcttc
     1321 tggaggtcaa cgaggggctg ctgcaccacg tgctgaagcg gttctcctgg gcgacggaac
     1381 cctatgagga cctcttccag gtggcgcggc tcgggctgat gaagtccgct caacggttcg
     1441 atccgcagcg agggcatctg ttctcgacgt atgccatagc catcatcgat ggagagatcc
     1501 ggcaccacct tcgcgattca cttctgatcc ggcagccgcg ctgggcccgc agcctctatg
     1561 cccgcattca agagacccaa acagccttct tcgccgagca ccaccgttcg cccaccgttc
     1621 ctgagttggc ggaacgcgtt aacatccggg aagagggtgt cctggagatc atccgtgtgt
     1681 acagcggcat cagcctgcac tcgctggacg aacctctcgg cagcccgcta cacgatgtcg
     1741 tcgacaagtc gctcgttcgc gcccttcggc cgcagaactt ctcgcttccg atcgaggacc
     1801 gcatcgtgct ctatgatgct ttgggagctc tatctgatct tcagaagaag atcatctact
     1861 tggtgttctt cggtgacctc acacaacagg aggtcgccga cgagatcggt ctttcgcagc
     1921 gtgccgtttc gcgcgagcag gtcaaggccc tcggacgact caaggccatc ctacacaagc
     1981 ggctcttcta ggctctttcg cctctctacg gcaacacttc cctaccctgg aactctatgc
     2041 acgataatgt atgttatgta tagtggaact gatgaccgta ccagactcag ccatgaccat
     2101 agaatcgacg cgcggaactc tcccccctcg gcgaagcgac gctcagtatg tgaccagact
     2161 gaagacgtcg tagccggcga gcttctttcg gccttcgagg aagccgagtt cgatcacgaa
     2221 tgccaacccg accaccgtgg cacccacgcg ttctaccagt tcgaccttcg cccttgcagt
     2281 gccgcccgtc gccaggagat catcgtggat gatgacacgt tgccccggct tgatggcgtc
     2341 gatgtgcatc tcgagcgtgt cgaacccgta ctccagctca tactccgctg tcgtgacctg
     2401 ccacggcaac ttgccgggct tcctggcggg cacgaaaccc gcgcctatgg agtatgcgac
     2461 agcggccccg aggatgaacc ctcgcgcctc tgctcccagg atcacatcgg cgttcttggc
     2521 ggcgctgaac tcggccaggg tgtccacggt gtaccgaaga gcgttcggat cctgaagcaa
     2581 cggcatgatg tccttgaaca tgatgcccgg cttcgggaag tccggcacat cacggatgaa
     2641 caccttcaga tccatcgatc tccttctttc gtcgggtagg ggtctcgtgc aggagctaga
     2701 tagccgcacg catcatccac tgtcgctccc atcgaagaca ggcagtggcc ggtcgctgcc
     2761 aggcggggtt caaagccatc aagcgaggcg gatcagttcc ttgtagagca gcaggtccag
     2821 gagacgggcc atcaaagagc ctagatcaac aaggaggcgt tctccctcct cgcgacgttc
     2881 tggatgggcc gagcgaagtc ctggagccgc tacttgagcg agcatggcag cttccctctc
     2941 agccgacgag cgcagaagcc gaagattccg cggttcgaca ccgtgccgcg caagaaggtg
     3001 gcagatgcgc actacttcca agtccgtgtc gctgagaatc ggatcaccga cggggtccct
     3061 gcgctcgaaa aggtggaaat cacccagcga acgcaggaag gcttccgaga cgcccgtgac
     3121 ttcaagcgcc tcctcccacg tgtgcgcgct ctcgtccttg agtaggcccc ggcgggcctc
     3181 gaacggtccg gacggcgcca acgccctgcc gaccacggaa gcggtgcctc ggtcgactct
     3241 ctccttgatg acctccagag gcaggaattc gtctcgctgg agcgtgagga ctgtacgcag
     3301 ggcccgcatc cccgctgcgt cgaacttccg gtatccgccg gcagtccgct cgggcctgac
     3361 gagaccccga tcctcgaggt acctgacctt gctgatggtc aaatctggga actcgccttg
     3421 cagagcctgc accacctgac cgacggtaag caggtggctg tccgctcctc tgcgatcgag
     3481 cggccctggc gccgggtccg aacggctcgc gggagcaggc ccgcccgaat cgccgctcat
     3541 gattccgacg gctccaggta ggcgaacttg aacttgccga tctgcacctc atcaccgtcg
     3601 atgaggtgtt ggcgctccac cctcccccga ttgacgtagg tgccgttgag gctgttcaga
     3661 tcctccacca cgaacccggt ggcgtcgtgg acgatgcgcg cgtgatgcct ggagacggtg
     3721 acatcgtcta ggaagagatg gctctcagga ttccggccga gcgtaagaag gtcgttgtca
     3781 agcaggatct gctcaccctc cctcccccct cccgccctga tgaggagagt cggcaccctc
     3841 ccaagggtgc gatcggtttg ggaaaggacc gcggctgcgt cgctggaggc cggcgcgtag
     3901 gtgacggtcg tctctgagag gtcacgctgc aggaggcgac tgccacaccg acagcagaaa
     3961 ttcgcgccct cttggttctg tgtgccgcac ttcgcgcagt cgttcactcc gccaccccgt
     4021 ctccgtaggc tatttggcga cgatagtcat ggaactctgc cgcgatgcga tcctcgatct
     4081 cgcgaagaat gtcagggctc tcaagaatgc ggagccggtc ctcagcgctg gtgtggttga
     4141 ccacgtaggg atcggccatc aattcatcga acggacggcc tccgtggagt tccatgacca
     4201 cgtatcgggt gacacgctct tctcgtacgt cgaccgagag gtcatggaac aaggcttgga
     4261 ccccggcaac aatgcgatcg atcctatcgc ccatcgcccc ctccgctcag acatcttcct
     4321 cagcgagccc tcgggaactg aggatggcac tcagcttctc cacgtctccc tcttcgaaga
     4381 gactctctcc cgtggagtgc ttgtcgcgca agcggcggac aatctccgca cgaaggatgt
     4441 cgagcttggc gtggatgatc cgtctcgtct tcgagacggc gcgctcctct tcagccagat
     4501 cctcagccga gcgcttcagt tcgtcgtcgc tcaggctctg gatgtgcagg agcagttcga
     4561 aagaagatgt gtcgtgctct cccatggctc aagtatactc ccgggccgag aaaccttcaa
     4621 cttcaactag aggtctacaa cgtgcgctcc atccatagac ggagcaggcc tggacttcgc
     4681 gtcgagacgt gcggaatcag gctgacgatg ccgggtcgga aggctactcc tcttgctccg
     4741 ccagcatctc ttcgtagtcc tcgggcgaca ggaggtcgtc cgcttcggat ggatcgctga
     4801 gcctgatctt gatgagccaa ccatcctcgt aggggctctc gttgatcagc tcgggagcat
     4861 ccacgacctc atcgttcgct tcgatcacag tcccactcag gggagcgaac acgtcagaga
     4921 cagccttgac cgattcgatc tcagcatagg aagtaccggc ggagacctca gcgccgactt
     4981 cgggtagctc gatgtacacg atgtcaccga gcgactcctg ggcgtagtcg gtcacgccaa
     5041 gagtcgcgac atctccttcg atgcgcgccc agtcgtgctc cttgtggtac ttcaggttct
     5101 tcggattcac atgcgctcct ttctggccaa ccgtggctgg cacccatcat aacacaggcc
     5161 ttggcgcccc ctgcgtggcg ccgaaacccc ctctcgtcgc gcccggactc cgtcgccgac
     5221 cgtctccttg cggccaccca acacatccag ccgcgccgga ttctaccagc ggccggggcg
     5281 cgtagcaaag ggtacagggc cttgttatac ttctgttcgt gtcccaggcc gtgttgaagc
     5341 ggctacaccc gcccctaagg aagtggtctt ggctcgtcgc aggggactcg tgatcaacaa
     5401 ccggcagatg cggctcgtgg acgacatcct ggagaaactg cgtcaggtat cgggctctcg
     5461 cttcattgcg ctcgtctcca ccagcgggca gccgatcacc acctctacag ttgaggggca
     5521 tcccgacacg ctctctttgg cctcgctggc ggccagttct ttcgcggcca cccagcagct
     5581 ggccgaggtg cttaacgagc gggagttcac cctcctcttc cacgaaggcc gcgagtcgaa
     5641 ccttcacgtg gcccaggtca cggaccaagt gctcctcgtg atcaccttcg gccgggagac
     5701 ccaggtgggg aaggtcaggc tctacaccaa tcgcgccgtc gaagtgctcc ggccggtatt
     5761 cgacgtcagc gaggacgacg accaggtctc cctgatgatc gatgatgact actctctcct
     5821 cgccgacgag gccatagacg acctgttcgc ggacatggag tgacagagtg ccactgatca
     5881 acttcgcggc cagggagatc aactacaaga tcgtctacta cggctgcggc ctgtgcggca
     5941 agaccacgaa catccaatac atccacaaga aggtgaatcc tgatgcccga ggcaagctcg
     6001 tgagcatcgc caccgaagag gagcgcaccc tctacttcga cttcctgcct ctctccctcg
     6061 gcacggtcaa gggcatgaag acccgcttcc atctctacac ggtccccgga cagtcctact
     6121 acaacgcgtc ccgccgcctc gtgctccagg gcgtcgacgg gatggtgttc gtggcggaca
     6181 gtcagatcac gcgtctcgac gagaacgtcg agagctactt gaatctctgg gacaatctac
     6241 tcgatcaagg cgacgatctc agcaagttgg ggttcgtgat ccaactgaac aagcgtgacc
     6301 tcagcgacat cttctcggcc gacgatatga gagaactctt gaaccacacg ggttcccctg
     6361 tgctcgaagc ctgcgcgctt agcggcatcg gagtattcga gactctgaag acggtgtgca
     6421 agcaggtcat cgccaagact ggccagagct gacgcccgag tcgcacgcac gaggcgggat
     6481 gcttcgtcgg ctgagagcct tctctgcgcc cgcctgctag ccccccacgc ggatcacgaa
     6541 gtctttcggc gagtacacgc tcgtgaagac gtcctcaaag agcactccgt atggatcgga
     6601 gaccacccgc cttacctcca cggacctgcc ggccaccccc ggctccactg cagacaccgt
     6661 tcccgatggg agcgccgggt cgcggagctt gcgtgggatg tcctcagtgc tcctgggctc
     6721 caccagatcg ttgaacggag acgtttcgta ggagatggtg cggtcccacg tcggcgcagc
     6781 cagggacacc gtcaccgatt gcgacgtggt cgagacggac aggaggagca cggcatccga
     6841 gtcgttgcgg aatctgaagt cggttgcgcc gtaggacacg gtggcgtcgc ggccaacggg
     6901 atagtgctcg atgtagaagg agtgcggctt cctctcgacc acgggaagtc cggcgaagaa
     6961 caccgcgttg aacagggtcg tggagacctg acagatgccg ccccccaccc cgggcgtgag
     7021 tgcgccgtcg cgaatgaccg gggcttcgtc gaaacccgcc gcgcgcgtgc gcgggcccac
     7081 agccgcgttg aatgagaagg tctctccggc gcgtatcacg tagccgtcca agatagtcgc
     7141 cacttgccgg atgttgttga ctcgagcgga gttcgcggca ctgaagtagg tggtgaacga
     7201 ggcgatctcc ctgcgctcct ggagttcctg cgcatcgagg gtggtgagtc ggggttgcac
     7261 ggtcgcggtg ggtatcggca cgtactttcg ccccccctcc agcaccacgc gcgccaggga
     7321 actgagcaag gccggccact cgatcagagt gccttcgacg ctgggcgtca ccgtgaagct
     7381 cttcccgtcg tcccctggga tgacctccgc ttctactggg gggtcttcca gggacttcaa
     7441 acgctcgccg agcacctcgc gggcggcgtc ggtatcgaat gtaagcggcg atccgttgac
     7501 gatgccggac ggatttgggg cggcgatcgc acctagttcc cccggcgaga cctcgaacag
     7561 tgttccttgg tatccgaggg tgaccggaga ggccaagccg gtccggacac tcgccaagac
     7621 agcttggacg tcttccgtgc gcacggccgg ttcgatctcg cggaggggcg ctcgcacctc
     7681 gggtcttcca tcggccatgg cctggataag ccctgcgcgt agaccgtccc tgtcgagggc
     7741 gacaccgacc tcggccgcca ccgccttcgg ccgcccttgc tcgaggatga gtcgtgcctc
     7801 cttgctttga cgctcaacca gcaaggcgac ttcgtcgaga gtggaggcca gacgtatgtc
     7861 gtcgacctca aaacgaaagg gcagggcaag ccggtcgcta tccccgaaga ctctgcccca
     7921 gatctcccgg aaaagtgacg tcgattccgt gcgggccagg ccgggggcca cactcccctg
     7981 cgcgtcgatg ctgaggccgg cctttgccgg attcacggtg aagacggcat ccccagcgac
     8041 cacccggatg ggacgctcca gcagcgacgc ggcggaggcg gccaccttcg ctgcggccgc
     8101 ctccgccgac aaacccccga cctcgacgcc accgactgag atgccccgcg gaacggtccc
     8161 aggggcgtcg cccgtgacga acgcgaaccc gaagacggcg gcgatcacga gtacccccgc
     8221 ggcggcgact gctggaagga ggcgccgcat agatcagtcg gttctcccgc cggcatcgtc
     8281 gatcgtctct tgcagcatat gcgcataccg ggccagcagt tcatccgacg ccgcgagcgt
     8341 gtccgcctcg gcgaacacgt ggaacacggg ttcgcccggg tcggggagga actgcaccca
     8401 gtcggcgctt cccacagcca acttcactcc atcgaggagt gacaccctct ggtgcttcaa
     8461 tctctcgacc aacatgcgca tcaccacgcc cttgacgctc caggggcact ccaccacggc
     8521 gtggcgcacc gtcacttcag gaagagcacc ggccagacgc gacacatgct ccatcgtcga
     8581 ggccagcatc tcaagcacca taaggaagct cacgagtgca tcgggcgccg gcaggaaggc
     8641 tgggagcacg aaccgccctc ccccactccc ggcgaaaatc gagtgcggtt tgcccgcttc
     8701 actgagcagg gcggactcag agaccttcgt gcgtacaacg ttgccgccgc actcagccgc
     8761 cacctcctcc gcgcgtcgcg tggtgtggag cgggaccacg atgttccccg gcccgtagtt
     8821 cgtgcaggcg tgcctgagta ggagcagcag cagcgagacg tcgggcacgc cggcaccagt
     8881 ctcatccaag acggtcaccg actccccacc cggatccatg agcacaccca cgtcggcttc
     8941 catggctcgg accaaccggc cgaccctctc ccctctgtcg accaggggcc gtgtcgcccc
     9001 atcagccccg tcttcccgtg gatctcgagt gttgacggat accacttcca gccggagcgc
     9061 gtccaacacg ctcgatgcca aaaccgtcgt gcccgagcca ccgtggtcca ggacgacacg
     9121 caggtcgcgg tcgcggatgc ggtcgatgtc gcaggcagtc agaagcgccc cgatgtacgt
     9181 ctcgatcagt ctcgcaggga agacgatcga acccatctca ctcggtgcca ctcggcgata
     9241 gtcttcgcgg tagaagatgt tctccatgcc acgttccctc tggggactca tgggaacacc
     9301 aggacgctcc gagagcacga tctcggcctc ttccggattg tccgcacaca cccggagatg
     9361 gactccaccc gccgaattgc ccaccttgag atcaaaacgg gtgagcgagc ctggggcgat
     9421 ctggaggtct cttaccgtca cccctgaggc gttcaaccct gcagtcaggg ccctgttgag
     9481 gacccgggcg gccgggtgct gatcccggct tgtcgtcacg atcgaccctg cgctcaagga
     9541 agtcccgtat gccataccca agcgcatgac caattccggc gtgatatcaa tgttgatgag
     9601 accgcgcacg ccctccttgc cgaacaggga actttgccca cgggattccc agatcaggct
     9661 cgaacggacc gaggctcccg actcgattac cttgcccggg tagaccttga cctgactacc
     9721 gaccactgcc ttctcgccta ccgaagagcc ctcaccaagc actgctccct cggcgatgga
     9781 agcgttcgcg cggatgtcga cccccgaccc gacgatcgcc ccccgcaccc gaaccccagg
     9841 acccaagtag gatccgtccc cgacgaccga acctgcgact gaggctccgc cacgcaggat
     9901 gacgttgttg ccgaggactg tgtgcgctcc gatggaggcg cttccagaaa ggcgggcgta
     9961 gtcaccgatg agagcagggc cttggatatt gtcgagactg tcgaagtccg ctccgtgacc
    10021 cacccacaca ttgcctcgca acctgacccc aggcaaattg gcctgcacct tgccatcgag
    10081 agcgtctctg ttcgcctgga ggaactgtgc caggcttccg atgtcctgcc agtagccttc
    10141 gcagagcatg ccgaacaacg gcttccgcga gtcgtagagg tgggggaaca gttggtggct
    10201 gaagtcgaag gagaccccct caggtatatg gtcgagcacc tccggttcga gcacgtagat
    10261 gcctgtgttg atggtgtcag agaagacctg cccccacccc ggcttctcca agaacctctc
    10321 gatgcggccg tgctcgtcga cgatgacgac gccgaactcc agcgggttgt cgacactctt
    10381 caaggctatc gtcaccatgg cgtctcgcgc ccggtggaag tcgacgatct cggtcaaatc
    10441 gaagtcggtg agcgagtcgc cgctgatcac caggaaggta tcgtccagga accgttgcgc
    10501 attcttgacc gaccccgccg ttccgagcgg cacttcctct acgcagtagt ctatctgcat
    10561 gccgaaggag gaaccgtcgc cgaagaagtt ctggatcaac tgaggcatga aggccaccgt
    10621 catgaccaca tcgcggatcc cgtggcggga gagaagttcg agaatatgct gcgcggtggg
    10681 cttgttcgcc accggcacca tcggcttcgg catgttcgac gtgagaggac gcaggcgagt
    10741 gccctgcccc ccggccatca ccacagcttt cacccgcagc ctcctcgccc tatgagcctg
    10801 tcggcgtgac tacccccgcc tccggacggc gaaactgacg gaagccccga agcgtgtagt
    10861 gcgcgcccga cgtcaatgag agagcggcgc cgacccacag tatgggaaca gcggccccga
    10921 taccgagaac cgctaggaac agcgcagcca tcagcaccgc cgtgcccgcc ttgccgagca
    10981 tgctcacggg cacgcggaga cctctgcgac tcaagacgta gtagccggcc atgacgtagg
    11041 tgtcccgcac gagcacgacc atgagaggag cggtgggtag acgatgcttc acgagaagaa
    11101 tgatcagggc gctcccgatg aggagtcggt cggccatcgg atccgcgaag cggccgaagt
    11161 cggtgaccgt atcggttctg cgagcgatct gcccgtcgag ccaatccgtg accgacgcca
    11221 ccgcgaagag gacgcccgct accaccgagc ggcccccgtc atctccggcc agatagtaga
    11281 tgaacgccgg gatcaggagc agccggaaca tggtaagcac gttggccagc gcacacctcc
    11341 gtgatcgcca ccagggccgc gtgcaacggg aatccttcca cgcccggcgg aagttcctcg
    11401 cactagagtg agacaaagtc gggacggcca gattcgaact ggcgacctcc cggcccccag
    11461 ccggatgcgc taccagactg cgccacgtcc cgacgcagcg gccagtatac cacccggagg
    11521 cgcggtcaat gtcgctcggt gcgcggctcc cgcgacataa gatcggccac gcaatcctgg
    11581 accgacttct gctcgtagac gaccgcgacg acgttgcggg tgatgggcat gtcgaccccg
    11641 tatttgcggg ccaacgcgag gatcgccggg gccgccgtga ggccttcggc caccatgccc
    11701 atctcccgct cgacctcgcc gggcgagtgc ccaagcgcga tgagttctcc ggcgaggcgg
    11761 ttccgactat ggtgagaggt acacgtggct accagatctc ccaggcctgc gagaccggcg
    11821 aaggtgagct gctccgcgcc caaggccact cccaagcgcg ccatctcggc aataccacgc
    11881 gtgatcaagg cggcacggac gttgtcgccg taccctatcc cgtcccccat cccggtggcc
    11941 agagcgatga tgttcttgac ggcgccggca agttccactc cgacgagatc gtggttgacg
    12001 taggcccgga agctctcgtt ggacaggagg agctgcaatg actctgcgta gctaggatcc
    12061 gcagacgcga tcacggttgc cgtaggctgg tcaagagaca cctcttcggc gtggttgggg
    12121 ccggccagca cggcgatcct cggctcgagg tgaccccaca cgtctgccag cagtacgctg
    12181 aacctgcgca gcgagcccgg ctccacgccc ttggtgagac tgaggacgtt cacgccctga
    12241 ggcaatcgat cgaccaagga gtcggccacc gccgcatacc ctttgctggg aaccgccatt
    12301 acgacaaggt ctacgtcttc gaggcttccc tctgtgtaga ctcccgcgtc aagggaagga
    12361 ggcagctgcg tatcccgaag gtatgagggg ttcatgcctg tggcacaaat cgcctccgcc
    12421 tgttccatgc gacgtcccag caggcgggtg cgcaggccca gaagggcgag atgacgtgcc
    12481 atcgcagtgc cccagctccc ggcgcccacg accgccaccc gtagggtgtc cccttccctg
    12541 ggcgccagtt cggggctctc actcatcagg cttactcctt tcggcgtcgc cggcgtcccg
    12601 ggcaggaact cggccgaggc t
//
LOCUS       DASRMJ010000277         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_105426, whole genome shotgun sequence.
ACCESSION   DASRMJ010000277 DASRMJ010000000
VERSION     DASRMJ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105426"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /gene="rny"
                     /locus_tag="VFD74_07660"
     CDS             <1..998
                     /gene="rny"
                     /locus_tag="VFD74_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HZK49459.1"
                     /translation="ARNVLSLCIQRTASSHVADTTVSVVSLASDDMKGRIIGREGRNI
                     RTLENLTGIDFIIDDTPEAVVLSGFDTVRREIARITLTKLIADGRIHPAKIEEMYTKA
                     KDEVENEIREAGEQATFEADIHGLAPELVKLLGRLKYRTSYGQNVLVHSLEVAYLAGL
                     MAAELGANAKLAKRAGLLHDLGKAVDHEVEGSHAVIGANLARRYGETEAVVHVIEAHH
                     NDVEPQTVESVLVAAADAVSGARPGARRETLEIYVKRLESLERIAESKKGVEKSYAIQ
                     AGREIRVMVKPEEVDDDMAALLSREIAKEIEQALDYPGQIRVTVIRESRAVEYAK"
     gene            1000..>2061
                     /gene="miaB"
                     /locus_tag="VFD74_07665"
     CDS             1000..>2061
                     /gene="miaB"
                     /locus_tag="VFD74_07665"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZK49460.1"
                     /translation="MRHVAAAAARVAAAADPSLSMDSVTQNTFYLRTFGCQMNEHDSE
                     RIAGVLEGMGYLAVSEPEEGALLVYNTCSIREKADTRLMGHLGAARRLKTEEPERVVI
                     VAGCLAQSRRDSFFAEHPYVDILVGPQSLHLLPALVEERQAGGPRAGAYEETTTHWSA
                     ELPRARRRGPVAWLQIMAGCSNFCSYCIVPYVRGPEASRAPDDIAAEARGLMRDGVKE
                     LTLLGQNVNAYGLEPGFSYDQDFPRLLGALSKIEGFERLRFMTSHPKDVSPALIEAMA
                     TLRPVCEHLHLPVQSGSDRVLAAMRRDYDVQSYLDLVARLRTAIPGLALTTDLIIGFP
                     GETDGDFQETLDLVEEVGFD"
BASE COUNT          389 a          643 c          695 g          334 t
ORIGIN      
        1 gggcaaggaa cgtcctctcg ctgtgcatcc agcggacggc atcgagtcac gtagccgata
       61 ccaccgtctc ggtggtgtcc ttggcgtcgg acgacatgaa gggccgcatc atcgggcgtg
      121 aaggccgcaa tatccgcacc ctcgagaacc tcacgggcat cgacttcatc atcgacgaca
      181 cgccggaagc tgtggttctg tcggggttcg acacggtgag gcgggagatc gcccgtatca
      241 cactgacgaa actcatcgcg gacggtcgca tccatcccgc caagatcgag gagatgtaca
      301 ccaaggcgaa ggatgaagtc gagaatgaga ttcgggaggc cggagaacag gctaccttcg
      361 aagccgacat ccacggattg gcgccggagc tcgtgaagct gttgggaagg ctcaagtacc
      421 gcacgagcta cggacagaac gtcctcgtcc actcgctgga ggtggcatac cttgccggct
      481 tgatggcggc ggaactcggc gcgaacgcaa agctggccaa gagggccggc ctgctgcacg
      541 atttgggcaa ggctgtggac cacgaggtgg agggctctca cgccgtcatc ggggccaacc
      601 tggcccgccg ctacggtgag accgaagcgg tggttcatgt catcgaggca caccacaacg
      661 acgtcgagcc gcagacggtg gagtcggtgc tggtagcggc cgccgacgcc gtttcgggag
      721 cccgccccgg agctcggcgc gagacactcg agatctacgt gaagcgtctg gagtctctgg
      781 agcgcatcgc cgaatccaag aagggtgtgg agaaatcgta cgccatccag gcggggcgtg
      841 agatcagggt catggtgaag ccggaggagg tcgacgacga catggcggcc ctgctgtccc
      901 gggagatcgc caaggagatc gagcaggcgc tggactatcc cggccagatc agggtcacgg
      961 tgatccgcga gagccgggcc gtcgagtacg ccaaataggg tgaggcacgt cgcggcggcg
     1021 gcggcgcggg tcgccgccgc cgccgacccg tctttgagta tggactccgt cacgcagaac
     1081 accttctacc tgcgcacctt cgggtgtcag atgaacgagc acgactccga acgtatcgcc
     1141 ggggtcctcg aggggatggg ctacctcgcg gtctccgagc cggaggaggg ggcgctgctc
     1201 gtctacaaca cctgctcgat ccgggagaag gccgacacgc gcctcatggg ccatctgggg
     1261 gcggcccgtc gactcaagac cgaggaaccc gagcgggtgg tcatcgtggc cggatgtctg
     1321 gcgcagagcc gtcgcgactc gttctttgcg gagcacccct acgtggacat cctcgtgggt
     1381 ccccagagcc tccatctcct tcccgccttg gtcgaggagc gacaggccgg cggtccccgg
     1441 gcgggagcgt acgaggagac caccacgcac tggagtgccg agctcccacg ggcgcgccgg
     1501 cggggccccg tcgcctggct ccagatcatg gccggatgca gcaacttctg ttcgtactgc
     1561 atcgtccctt acgtcagagg gcccgaagcc agccgagcgc ccgacgatat cgcggcggag
     1621 gcccgggggt tgatgcgcga cggagtcaag gaactcacgc ttctcggcca gaacgtgaac
     1681 gcctacggtc tggagcccgg gtttagctac gatcaggact tcccaaggct gctcggagct
     1741 ctgtccaaga tcgagggttt cgagaggctc cggttcatga cctcgcaccc caaagacgtc
     1801 tcaccagcct tgatcgaggc tatggcgacc cttcgcccgg tatgtgagca cctgcacctt
     1861 cccgtgcaat cgggcagcga tcgcgtgctc gcggcgatga gacgtgacta cgacgtccag
     1921 agctatctcg acctggtcgc gcgattgcgc acggcgattc ccggactggc tctgaccaca
     1981 gacctcatca tcgggtttcc cggtgagacc gacggcgact tccaggagac cctcgacctg
     2041 gtcgaggagg tgggcttcga c
//
LOCUS       DASRMJ010000278         4869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_533151, whole genome shotgun sequence.
ACCESSION   DASRMJ010000278 DASRMJ010000000
VERSION     DASRMJ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533151"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /gene="holB"
                     /locus_tag="VFD74_07670"
     CDS             complement(<1..602)
                     /gene="holB"
                     /locus_tag="VFD74_07670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZK49461.1"
                     /translation="MPLFADILGQPLALTSLRQALLHGASHAYLFAGPAGVGKSETAL
                     AFAAGLACPERGCAACETCRRVLEGLHPDVEIVSPEGAVIPIGQIREINREVVMRPFE
                     ASVRVFIILDAHAMNAEAANALLKTLEEPPRHAHFVLVSDSPQRLPQTVVSRCQRVPF
                     TSVPSPLLATHLREAYGLSELEALAFARASHGSPAHARALA"
     gene            complement(646..1401)
                     /gene="tmk"
                     /locus_tag="VFD74_07675"
     CDS             complement(646..1401)
                     /gene="tmk"
                     /locus_tag="VFD74_07675"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZK49462.1"
                     /translation="MADVAPGGSAGACGGRSQREWRPPPADAALDESALAGLGSATRG
                     VFITFEGLDGSGKSTQMDLLAGSLRAAGYEVVTTREPGGTPLGEAFRDILLDPGHSAM
                     TARAEALLYSAARAQLVETVIRPALDEGRIVLCDRYLDSSLAYQGYGRGLQVEDVLTL
                     NVWATECLFPDLTIVLMVDEVERANRASQAPDRLEAAGLSFFERAAEGYRRLAADHRH
                     RIRLVDSVGAVNEVQARVRAAIDEELELFSRSS"
     gene            complement(1505..2395)
                     /locus_tag="VFD74_07680"
     CDS             complement(1505..2395)
                     /locus_tag="VFD74_07680"
                     /inference="COORDINATES: protein motif:HMM:NF017967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HZK49463.1"
                     /translation="MSLAIKPRVTPALTATVFLVVLLAGALSLALAGPARADSTEVVT
                     LGADLSAAQRQEMLDLFGVSEGDLPVLEVTNKEERDYLQGLISDAQIGTRSISSVYVR
                     LKDDGSGIAVQTSNITYVTEQTYANALVTAGIADADVFAAAPFPVSGTAALTGVFKAF
                     EEATGKTISAAAKEAATQELVDTAEVGDQVGDKDAIAELVKLAKEEIVSRGLNDEASI
                     REVLVRISGQLDLRLTDEQLDRLAKVLVQVQGLHLDLSKVQSQLKDFQTRIGVSGDEA
                     KGIWESIKGLFSKIWEAIFG"
     gene            complement(2414..4660)
                     /locus_tag="VFD74_07685"
     CDS             complement(2414..4660)
                     /locus_tag="VFD74_07685"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HZK49464.1"
                     /translation="MRLIITEKNDVASKLAGILSQGAIERDAFYKIPYYVFTDATGRR
                     NTAVGLKGHVMQVEFPPEYADWRKVDPSSLIDAPLVKGETAKSVVRAVRKLAGEADEL
                     VIATDYDREGELIGLEARDIAAGENESLLRHLRRARFSALTAGEITRAFDSLEYLSEP
                     LAWAGEARQDIDLVWGATLTRFVSLATYRLGNQFLSVGRVQTPTLALVVAREKERRAF
                     VPEPYWVLTADLAADAQRFTATHKVERFLDQHAAEAAYEKLTGPARVTQLKRTSRKVP
                     RPAPFNTTSFITSATSVGLSASAAMRVAEDLYMGGYISYPRTDNTVYPGSLDLREILH
                     TLTESEFAADARKLLALPKLSPSRGKKKTTDHPPIYPTGAARHGALDDRARKVYELVV
                     RRFFATLADDAISESNRIDLDIDGEPFFVRGSRVTYAGWLAFYPYSRQKDAELPDLAE
                     GQEAQLVGKNLDGKETQPPSRYGQGPLIELMETHNLGTKATRHNIIQNLYDRGYIHGN
                     PVEPTETGVKMAEALLEYAPLIASPEMTAQLELDMDAIAEETQTKEHVVATSRALLHK
                     AYKSLAENREAVAAKIFEGITDDRILGDCPTCGTHKVRVIKSKTTRKRFVGCEGYPEC
                     TQTYPLPQRGDILATGETCPSCGTPRIKILGGKRPWILCLDPECPTKAEYREKQAARL
                     AARGSAAAAKKPAAKKSAAAKKPAAATAAAAKKTTTVAKTTVAKKPAARKKATPAAGT
                     PSAEDQNQ"
BASE COUNT          766 a         1591 c         1662 g          850 t
ORIGIN      
        1 gccagtgccc gagcgtgggc cgggctgccg tgggacgccc gggcaaacgc caaggcctcg
       61 agctcgctca ggccgtaggc ctcacggagg tgggtcgcca gcaacgggga aggcaccgac
      121 gtgaagggca cccgctgaca gcgggacacc accgtctgcg gcagcctctg cggtgagtcg
      181 ctgaccaaga cgaagtgggc gtgtcgcggc ggctcctcga gcgtcttgag cagggcgttc
      241 gcggcttcgg cgttcatcgc gtgggcatcg agaatgatga acacccggac cgaagcttcg
      301 aagggccgca tgaccacctc gcggttgatc tcgcggatct gccctatcgg gatgacggcg
      361 ccctcggggg agacgatctc cacgtcggga tgcagcccct ccaagaccct gcgacacgtc
      421 tcgcaggcgg cgcaccctcg ctcaggacag gcaagtccgg cggcgaaggc cagcgcggtc
      481 tcgctcttgc ccacacccgc cgggcccgcg aagagatagg cgtgggaagc gccgtgcaag
      541 agcgcctgcc gcagggaagt cagggcaagc ggttggccca ggatgtcggc aaaaagaggc
      601 acgcagcaag tgtagcaggg ggaacggccg tgacggacgc caccctcagg aactccggga
      661 gaagagctcc aactcctcgt cgatggccgc gcgcacccgt gcctgcacct cattcacggc
      721 ccccacagag tccacgagcc ggatgcgatg tcggtggtcg gcggccaggc ggcgatagcc
      781 ttcggccgcc cgctcgaaga acgacagccc ggccgcctcc aggcggtcgg gcgcctgtga
      841 ggcccggttc gcccgttcga cctcgtccac catgagcacg attgtcaagt cggggaagag
      901 acactcggtg gcccagacgt tgagagtcag cacgtcctcc acctgcagac cgcggccgta
      961 cccttggtag gccagagacg aatcgaggta acggtcgcag agcacgatcc tgccttcgtc
     1021 cagagcaggc cggatcaccg tctcgaccaa ttgggcgcgg gccgccgagt agagcagagc
     1081 ctccgccctg gccgtcatcg ccgagtggcc ggggtcgagc aggatgtcgc ggaaggcctc
     1141 gcccaacggg gtgccgcccg gctcacgagt ggtgaccacc tcgtagccgg cagcccgcag
     1201 ggacccggcc agcaggtcca tctgggtgct cttgccggag ccgtccagac cctcgaacgt
     1261 gatgaacacg ccccgcgtgg cgctgcccaa gcccgccagc gcactctcgt ccagcgccgc
     1321 gtcggcgggc ggcggcctcc actctcgctg agagcgacct ccacacgcgc ccgccgaccc
     1381 gccgggggct acgtcagcca aggatggcct cggaaatacc gggggctacg tcagccaatg
     1441 atggtgtcct ggatgccggc ggctgcatca gccaatgatc ggctcgcgga tgccggcggg
     1501 tgcatcagcc aaagatggcc tcccagatct tcgagaacag acccttgatg ctctcccaga
     1561 tgcctttggc ttcgtcgccg ctcacaccga tgcgggtctg gaaatccttg agttggctct
     1621 gcaccttgct cagatcgagg tggaggccct gcacctgcac caagaccttg gccagccgat
     1681 cgagttgctc gtcggtgagg cgcagatcga gttgcccgga gatgcgcacc aggacctcgc
     1741 ggatcgaggc ctcgtcgttg agcccgcggc tgacgatctc ttccttcgcc agcttcacga
     1801 gctcggcgat ggcgtctttg tcgcccacct ggtcgcccac ctcggccgtg tccaccaact
     1861 cctgggtggc ggcttccttg gccgccgcgg agatagtctt gccggtggcc tcctcgaagg
     1921 ccttgaagac gcccgtgagc gcggccgtgc ccgacaccgg gaagggcgcg gcggcgaaga
     1981 cgtcagcgtc ggcgatacct gccgttacca gggcattggc gtaggtctgc tcggtcacgt
     2041 aggtgatgtt gctggtctga accgcgatgc ccgacccgtc gtccttaagc cgcacgtaca
     2101 cactcgagat cgagcgcgtg ccgatctgag cgtcggagat cagcccctgc aggtagtccc
     2161 gttcctcctt gttggtgacc tccaacacgg gcaggtcgcc ctcagacacg ccgaagagat
     2221 cgagcatctc ctggcgttgg gcggccgata gatcggcgcc gagggtgacc acctcggtgg
     2281 agtcggctcg tgccgggccg gccagggcca ggctgagggc cccggcgagc aatacgacga
     2341 ggaataccgt ggcggtgagc gcgggagtta cccgcggctt gatggctagc gacatgatcg
     2401 gcctcccttg atgctactga ttctggtctt ctgccgaagg cgtgcctgcg gctggggtcg
     2461 ccttctttcg ggctgcgggc ttcttggcga cggtggtctt cgcgaccgtc gtggtcttct
     2521 tcgcggcggc ggcggtggcg gccgccggct tcttggcggc tgccgacttc ttggccgcgg
     2581 gcttcttcgc ggcagccgcg gagccgcgag cggccaggcg cgcggcctgc ttctctcgat
     2641 actccgcctt ggtgggacac tccgggtcga ggcacaggat ccaaggacgc ttgcctccga
     2701 ggatcttgat gcgcggcgtg ccgcacgacg ggcacgtctc gccggtggcg aggatgtctc
     2761 cgcgctgggg caacggatag gtctgcgtgc actccgggta gccctcgcat ccgacgaacc
     2821 tcttacgcgt ggtcttggac ttgatcacgc ggaccttgtg ggtgccgcag gtgggacagt
     2881 ctcccaggat gcggtcatcg gtgatgccct cgaagatctt tgccgcgacg gcttcccgat
     2941 tctcagccaa ggacttgtat gccttgtgca gcagcgcgcg cgaagtggcg accacgtgct
     3001 ccttggtctg ggtctcctcg gcgatggcgt ccatgtcgag ttcgagctga gccgtcatct
     3061 cgggcgacgc tatcagcggc gcatactcca agagcgcctc tgccatcttc acgcccgtct
     3121 ccgtcggttc caccgggttg ccgtggatgt agccgcggtc gtacaggttc tggatgatgt
     3181 tgtgcctggt ggccttggtg cccagattgt gggtctccat gagctcgatc agcgggccct
     3241 gcccgtaccg tgaaggtggc tgcgtctcct tcccgtccag attcttgccc accagctggg
     3301 cctcctggcc ctccgcgagg tcgggcagtt cggcgtcctt ctgccgggag tacgggtaga
     3361 acgcgagcca gcctgcgtat gtgacccgcg agccccgcac gaagaagggc tctccgtcga
     3421 tgtcgaggtc tatccggttc gattccgaga tggcgtcatc ggcgagggtg gcgaagaacc
     3481 tccgcacgac gagctcgtac accttgcgcg cccgatcgtc cagggctccg tggcgggcag
     3541 cccccgtagg gtagatggga ggatggtcgg tggtcttctt cttgccgcga ctgggcgaga
     3601 gcttcggcag cgccagtagc ttccgcgcgt cggcggcgaa ctcgctctcg gtcaaggtgt
     3661 gcaggatctc gcgaagatcg agcgatcccg ggtacacggt gttgtccgta cgcgggtagc
     3721 tgatgtagcc gcccatgtac aggtcttcgg cgacgcgcat ggccgccgac gcgctcagac
     3781 ccacgctcgt ggcggaagtg atgaacgacg tggtgttgaa aggcgccggg cgcgggacct
     3841 tgcgactcgt gcgcttcagc tgcgtgacgc gagcgggccc ggtgagcttc tcgtaggctg
     3901 cttcagccgc atgctggtcg aggaagcgtt ccaccttgtg ggtggccgtg aaccgctggg
     3961 cgtccgccgc aaggtcggcc gtgaggaccc agtagggctc gggcacgaag gcccggcgtt
     4021 ccttctctcg ggccacgacc agcgccaggg tgggggtctg gacgcggccc acactcagga
     4081 actggttgcc cagacgatag gtggccagac tcacgaaacg ggtgagcgtc gcgccccata
     4141 cgaggtcgat gtcttgacgt gcttctcccg cccacgccaa cggctcggag aggtactcga
     4201 gtgaatcgaa ggcccgcgtg atctcccccg ccgtcagagc cgagaagcgg gctcttcgca
     4261 aatggcgcag gagcgactcg ttctcgccgg cggctatgtc gcgggcctcg agtcctatca
     4321 attcgccttc gcggtcgtag tcggtggcga tcacaagctc atcggcctcg ccggccagct
     4381 tgcgcacggc ccgcaccacg gacttcgcag tctcgccctt caccagcggc gcgtcgatga
     4441 gggacgaggg atcgaccttg cgccaatccg catactcggg ggggaactcc acctgcatta
     4501 cgtgaccctt gaggcccacg gcggtgttcc gccggcccgt ggcgtcggtg aagacatagt
     4561 acgggatctt gtagaaggcg tcgcgctcga tggccccttg ggacaggatg cccgcgagct
     4621 tcgaggctac gtcgttcttc tcggtgatta tcagtcgcac gtttcactac tccagacact
     4681 cgggcgctcg gcacttcacc gcacccaccc ggtggcggga catcgcgacc agcggcgacg
     4741 ggtaaagata cagacgggcg gcttctagtt caatctgcgg aagggtgggg tgggaacgcc
     4801 gcagggacgc gccggggcgg atcagacggc tgcgagggcc tcgtcgaccg tgcggtggat
     4861 cccgaacat
//
LOCUS       DASRMJ010000279         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_105950, whole genome shotgun sequence.
ACCESSION   DASRMJ010000279 DASRMJ010000000
VERSION     DASRMJ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105950"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1505
                     /gene="purH"
                     /locus_tag="VFD74_07690"
     CDS             9..1505
                     /gene="purH"
                     /locus_tag="VFD74_07690"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZK49465.1"
                     /translation="MELFARGLADLGIELISTGGTFKFLSEVGIPVKQVSEVTGFPEI
                     MDGRVKTLHPAIHGGILADRHNHEHLKAIQELGIEPIDLVVVNLYPFEDVAERRGASD
                     EEIIENIDIGGPTMVRAAAKNHTGVAIVVDPRRYGDVLRELRENEGALSEETRRDLAM
                     EAFHHTAHYDFAIANWFSSHAGDFPPYVMWDFKKVQDLRYGENPHQRAAFYADVDGRK
                     TIAPQLEQLHGTHLSFNNLLDLDSGRRLVKEFSLPCCVIIKHNNPCGVALAEGITLAY
                     EKALSCDPVSAFGSVIVVNRPVDETLARSLSTNFVEVLFAPAFLEEALEILTKKPDIR
                     LLVHEDHNRNHRGLYDLKKVIGGALIQERDSDSESRESMQVVTSRHPTEEEWGDLIFA
                     WRVAKHVKSNAIVLAKDLMTIGVGAGQMSRVDSARIAVQKARHPLAGCAVASDAFFPF
                     TDALEVAVEAGAASVIHPGGSKRDDDVTRYAEGQGMAMVVTGRRHFRH"
     gene            complement(1585..1893)
                     /locus_tag="VFD74_07695"
     CDS             complement(1585..1893)
                     /locus_tag="VFD74_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49466.1"
                     /translation="MATDRDKIAMLVRHWVEHNEGHRGSYLEWRDKLSDQDLPVTLAA
                     LQEVADLTDRANEALRRAAAELAGGEVTAAPEQAAHTHEHTHPHTHEHTHEHEHSHEH
                     "
     gene            complement(1930..2385)
                     /locus_tag="VFD74_07700"
     CDS             complement(1930..2385)
                     /locus_tag="VFD74_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49467.1"
                     /translation="MNFYWRQTIVGLLRYAAEQIPEIAARCLTTARELDRTLSTPWLE
                     EAFELFQRAALLSRNTVYVTGAYYSMRALEEVGRFEERTTGPLRTIVAESGEPGRSRG
                     RPDILPADTEAVKEAQGGILELFERIRRGATLEEALALFPLKGDESSPD"
BASE COUNT          438 a          817 c          827 g          450 t
ORIGIN      
        1 aggggaatct ggagttgttc gcccgcggcc tcgccgacct gggcatcgaa ctcatatcca
       61 ccggcggaac gttcaagttc ctgagcgaag tcggcatccc ggtcaagcag gtgtccgagg
      121 tcaccgggtt cccggagatc atggacggcc gggtgaagac gctgcacccc gcgatccacg
      181 gcggtatcct ggccgaccga cacaaccacg agcacctgaa ggccatccag gagctgggca
      241 tcgagcccat cgatctggtg gtcgtgaacc tgtatccctt cgaagacgtg gccgagcggc
      301 gtggcgcctc cgacgaagag atcatcgaga acatcgacat cggcgggccc accatggtgc
      361 gcgccgccgc caaaaaccac acgggtgtgg ccatcgtcgt agatccgcgt cgttacggcg
      421 acgtgctgcg ggaactccgc gagaacgagg gtgccctttc cgaggagacc cgccgtgacc
      481 tggccatgga agccttccac cacaccgctc actacgattt cgccatcgcc aactggttca
      541 gttcgcacgc aggcgacttc ccgccctacg tcatgtggga tttcaagaag gttcaggatc
      601 tgcgctatgg agagaacccg catcagcgtg ccgccttcta cgccgatgtc gacgggcgca
      661 agaccatcgc cccgcaactc gagcaactcc acggcacgca cctgtcgttc aacaaccttc
      721 tcgaccttga ttccgggcgc cgtctggtga aggagttctc tctgccctgc tgcgtcatca
      781 tcaagcacaa caacccgtgc ggggtcgctc ttgccgaggg cataaccctg gcctacgaga
      841 aggcgctgtc gtgtgacccg gtgtcggcct tcggcagcgt gatcgtggtc aaccggcccg
      901 tcgacgagac cctggcccgc tcgctcagca cgaacttcgt cgaggtgctc ttcgcgcctg
      961 cattcttaga ggaagccctg gagatcctta ccaagaagcc cgacatccgc ctgctcgtgc
     1021 acgaagatca caaccggaat caccgcggtc tctacgatct caagaaggtc attggcggcg
     1081 cgctcatcca ggagcgggat tcggacagcg agagtcggga gagcatgcag gtggtgacca
     1141 gccgtcatcc caccgaggaa gagtggggcg atctgatctt cgcgtggcgg gtggcgaagc
     1201 acgtgaagtc gaacgccatc gtgctcgcga aggacctcat gaccatcggt gtcggggccg
     1261 gccagatgag ccgtgtggac tcggcacgca tcgccgtgca aaaggccagg cacccgctcg
     1321 cggggtgcgc ggtggcgtca gacgccttct tccccttcac cgacgctctg gaagtggcgg
     1381 tggaggccgg agccgcgagc gtcatccatc caggcggctc gaaacgcgac gacgacgtga
     1441 cgcggtacgc ggagggccaa ggtatggcca tggtcgtcac gggacggcgg cacttcaggc
     1501 actagagacg gcggcgcacc cgggccgagt gggcgggggc gcacaagcac ggacggcagc
     1561 cgacgagtca gcggcgccgc ccgactagtg ctcgtgactg tgttcgtgct cgtgcgtgtg
     1621 ctcgtgggtg tgcggatggg tatgctcgtg cgtgtgagcg gcctgttccg gggcagccgt
     1681 cacctctccg ccggcaagtt ctgccgcggc gcgccgtaga gcctcgttcg cccgatccgt
     1741 caagtcggcg acctcctgca gtgcggccaa cgtcaccggc aggtcctggt cgctgagttt
     1801 gtcgcgccac tcgaggtagc tcccacggtg tccttcgttg tgctcgaccc agtgccgcac
     1861 caacatcgct atcttgtctc gatcggtcgc catgctgctc ctccgtttcg ccgtcggcgg
     1921 cgtgtcggct cagtcggggc tggactcgtc gcccttgagg ggaaacagcg ccagcgcctc
     1981 ttccagtgtg gctccgcgcc ggatccgttc gaacagttcc aggatccctc cctgggcctc
     2041 tttgacggcc tcggtgtccg cgggaaggat atcaggcctt cctcgtgagc ggccgggctc
     2101 accgctctcg gcgacgatcg ttctcagcgg ccccgtggtc cgttcttcga agcggcccac
     2161 ctcctcgagc gcccgcatcg agtagtaggc tccggtcacg tacacagtgt tgcgggagag
     2221 cagcgcggcc cgctggaaca gctcaaaagc ctcctcgagc cagggggtgc tgagagtgcg
     2281 gtcgagttcg cgggcagtgg tgagacaccg cgcggcgatc tccggtatct gttccgcggc
     2341 gtagcggagt aggccaacga tcgtctgtcg ccaatagaag ttcacgtgcc gcctcgccat
     2401 cgaacgggga ttggttaatg tcggcagagg ccgtagtcat ggaggctcgg cctactggtg
     2461 agtaacagat accaggggac ggttcggccc aaacggacaa gcggtcgcgg cgctcgcgtt
     2521 gcaggctcga gg
//
LOCUS       DASRMJ010000280         7595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_533463, whole genome shotgun sequence.
ACCESSION   DASRMJ010000280 DASRMJ010000000
VERSION     DASRMJ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533463"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFD74_07705"
     CDS             complement(<1..544)
                     /locus_tag="VFD74_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49468.1"
                     /translation="MSDTEISDALRASLSYRVAASVTRSLDAAVRGIHQRSRREGRGS
                     LAAVQASRSFRSLAACVSGIHRLQGIMRAAGARSALFGGGTGRVLPLARALAKLDRWL
                     VLIPFNFLFLDYAIRDLGPLQAFSNIWDEAVLLCLAVYVVLRPLWLETSYRLRWGDLD
                     WLVVLFIGLGVGLLGFVSPEP"
     gene            complement(561..1034)
                     /locus_tag="VFD74_07710"
     CDS             complement(561..1034)
                     /locus_tag="VFD74_07710"
                     /inference="COORDINATES: protein motif:HMM:NF025582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HZK49469.1"
                     /translation="MRRLPRTARMFDWLVLVVAVVIVLGIAYKVTNGRIDPSATGPEN
                     VPATVQLEVYAPEAGMLEHIRVGDRLAEGKDDIDGTVAAVELTPPSADGLVIDQNGVN
                     FAASGQARALVTIDAVLEKSDPTVSLGNQELRVGKLIFLESMLYKFTATVVKVEY"
     gene            complement(1046..1528)
                     /locus_tag="VFD74_07715"
     CDS             complement(1046..1528)
                     /locus_tag="VFD74_07715"
                     /inference="COORDINATES: protein motif:HMM:NF025582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HZK49470.1"
                     /translation="MKRFWTWFNWVDVLVVVLILVSAVAILERDRILGNVGSGTQVTS
                     GEKAIVVTMRTASVPLSTTKGFGVGDPLLAGRDIGNARIVSVRYEPSKVYVLRDGSVS
                     RTASNDLCDLYVAFEVVANSSGPYLEWAGQELKVGLVYIMKTPRAEAVGRIVAIDVLE
                     "
     gene            complement(1537..2649)
                     /locus_tag="VFD74_07720"
     CDS             complement(1537..2649)
                     /locus_tag="VFD74_07720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZK49471.1"
                     /translation="MQIVHIVNGADIGGAMTQVIALSGAQRKSGTVELIAIGPGKVIA
                     NGRRLGIPVRECAMRPAALRRLVRTLREKHDQGWVIHVHGLKPMVMAAIAARGEPLHV
                     AATIHSDFRQEYADSHYRSAVALPLIRWAFGRIERFATVSYELLELLREEGVSPDRRE
                     HIPNGVDLSLVAPTMSRQEFLRKRCVPDEGQVICGLAARLHPMKGIDLLIEVAHRFKG
                     EPVQFVVAGSGDPTLMADYREHIRVLGLADQVHMVGFVENIYDFYQAIDVNLLTSYSE
                     GVSYSVMEGAALGRPLVCTAVSGMESLVQDEVDGLKVPVGDADAFAAALRRLVDDPAL
                     RHTLGGRSREKVINDYSSEVMARRYDDLYRRLLQVA"
     gene            complement(2618..3895)
                     /locus_tag="VFD74_07725"
     CDS             complement(2618..3895)
                     /locus_tag="VFD74_07725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK49472.1"
                     /translation="MNKLLMILNEFPPIGGSGVQRSTKFVKYLPAVGYAPYVVTRAYS
                     GGLRDESLLKDLPEGLHRLDLERPDWVDGTGVIGRVRRLIGTRLMVPDAEVFWYLRHR
                     ERVLAWLDAEHIDLLYSTSYPYSSHLMALYLKRRRPHVAWVADFRDEWTNNPYSRDQL
                     LKRLRFPAERRMEKAVVAGCDYLVANTPRMLENFVADTPGVRDRSAAIPNGFDPDDFA
                     DYAPRNQGNEKFRIVYAGALYGRRRPDYVLQALAELIASADVDPARLEVEFVGNIHED
                     RIRRLVAGDALDAVVKVTPYMAHGSLMGHLAAANVLLLIESEVNFATGKVFEYIRMGI
                     PIVATVPLEGEAARVVRETRTGAAVDFADVAGIRDALAASYRAWCDGRASFAPEAAII
                     QRYSRRVQTEALGRCLEAAQRNRDADSPHRQRR"
     gene            complement(4172..5083)
                     /locus_tag="VFD74_07730"
     CDS             complement(4172..5083)
                     /locus_tag="VFD74_07730"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK49473.1"
                     /translation="MARRKIPAAAWPFRLSPDTPPDRVAEIREGVAALDSAAQYGWGH
                     SIDFGPFRKQGLLGDNYLDVAGMLDEWGWWPDDLSGMRVADVGCFTGGLSLLMAARGP
                     SIVYAIDEVGAHLDQAVFLAEVFEQTSVRAVKASVYDLIDHIPENSLDLVLLSGVLYH
                     LGDMLLGLHILRRLLKPDGLLLIESNAVNDNKRSFANFGRFYRGMWWQPSGLCISDMC
                     EFMGFNRAEVRFYGPDRCLARTRRSSDEDIPFKRGLNWHFESLADAQFRSTDPGIMAP
                     VRSYANWKHFLRGLPRKVSRRLRLPRS"
     gene            complement(5269..5574)
                     /locus_tag="VFD74_07735"
     CDS             complement(5269..5574)
                     /locus_tag="VFD74_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZK49474.1"
                     /translation="MYARAVNIQFHSAKIDEANRIVQDAIVPVLKEQKGFREQFLLTQ
                     SDTGKAVSINLWETEADLTAFESSQLYRELMGKLAGVIAAPPAGERYEVSVLARVQA"
     gene            5731..5964
                     /locus_tag="VFD74_07740"
     CDS             5731..5964
                     /locus_tag="VFD74_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZK49475.1"
                     /translation="MQQTTFTDFRRHAKDYFDAVEGGDVVRVYRNGRPIADVVPIPAE
                     EPAWKHPVVRIAVRGPRASDMVIQDRAEDPSPE"
     gene            5961..6374
                     /locus_tag="VFD74_07745"
     CDS             5961..6374
                     /locus_tag="VFD74_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZK49476.1"
                     /translation="MRVFFDASALVKRYVDEPGSDTVSAILEAAEVLIVSALCLPECV
                     SAFQRMASEGLLSHQEYVWLKDAVLADLADAIVCHVVPEVVDGAVECLERHPVRTLDA
                     IQIACARAVTADLFVTADRRQGAAARGEGLEVRVV"
     gene            complement(6690..6839)
                     /locus_tag="VFD74_07750"
     CDS             complement(6690..6839)
                     /locus_tag="VFD74_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49477.1"
                     /translation="MTQLPRASGREVVAALSKVGYEKDRSGRARSSGDRSIAGLTVAE
                     FKQLL"
     gene            complement(6836..7048)
                     /locus_tag="VFD74_07755"
     CDS             complement(6836..7048)
                     /locus_tag="VFD74_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZK49478.1"
                     /translation="MKYRVLIQQGVDGVFVVEVPSLPGCISQGQTRSEAMENIREATA
                     AYLESLEAHGEPIPPSIDEEVIEVAV"
     gene            complement(7161..7252)
                     /locus_tag="VFD74_07760"
     tRNA            complement(7161..7252)
                     /locus_tag="VFD74_07760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7214..7216),aa:Ser,seq:tga)
BASE COUNT         1312 a         2437 c         2485 g         1361 t
ORIGIN      
        1 caggctccgg ggagacgaag cccagcagtc ccacccccag cccgatgaac aggaccacca
       61 gccagtcgag atccccccac cgcaggcgat agcttgtctc gagccacagc ggcctgagca
      121 ccacgtagac cgcgaggcag aggagcaccg cctcgtccca gatgttcgag aaggcctgca
      181 ggggaccgag gtcccggatc gcgtagtcca agaagaggaa gttgaacggg atgagcacca
      241 gccaccggtc gagcttggcg agtgcgcggg cgagtggcag cacgcgaccg gtgccgccgc
      301 cgaagagggc gctgcgggcg ccggcggccc gcatgatgcc ctggagccga tgtatgcccg
      361 agacgcacgc cgccagcgag cggaacgacc tgctggcctg caccgccgcc agcgacccgc
      421 gcccctctcg acggctccgc tggtggatcc cgcggacggc ggcgtcgaga ctccgcgtca
      481 ccgacgccgc tacacggtac gagaggcttg ctctcagggc gtccgatatc tcagtgtccg
      541 acatctcggc ggccgccacc tcagtactcg accttgacca ccgtggccgt gaacttgtag
      601 agcatggact cgaggaagat caacttcccc acccgtagct cctggttccc caggctcacg
      661 gtcggatccg acttctccag caccgcgtcg atggtcacga gggctcgggc ctgacctgac
      721 gcggcgaaat tcacgccgtt ctggtcgatg acgaggccgt cggcgctcgg gggagtcagc
      781 tccacggcgg ccacggtgcc gtcgatgtcg tccttgccct ccgcgagccg gtcaccgact
      841 cggatgtgtt cgagcatgcc cgcttccggt gcgtagactt cgagttgcac cgtcgccggc
      901 acgttttccg gccctgtggc gctcgggtcg atgcgaccgt tcgtgacttt gtaggcgatc
      961 ccgaggacga tcacaacggc gacgaccagg acaagccagt cgaacatgcg ggcggtgcgg
     1021 ggcagacgtc tcatgtgggc ctatcctatt ccaacacgtc gatggcgact attctgccga
     1081 ccgcctctgc gcgaggcgtc ttcatgatgt acacgagccc caccttgagt tcttggcctg
     1141 cccactccaa gtacggcccg ctgctgttgg caacaacttc aaaggcgacg tagagatcgc
     1201 aaaggtcgtt cgacgcagtg cgggaaaccg agccatccct caatacgtac actttggatg
     1261 gctcgtagcg caccgacacg atccgcgcgt tcccgatatc ccgccctgcc aagagagggt
     1321 cgccaacgcc gaaacccttc gtcgtggaca gcggcaccga ggccgtcctc atggtgacga
     1381 cgatggcctt ctctccggag gttacctggg tgccggaacc gacgttgccc aggatgcggt
     1441 cgcgctcgag gatcgcgacc gccgagacca ggatcagcac gacgacgagc acgtccaccc
     1501 agttgaacca tgtccagaat cgtttcatgt ctctccctaa gcgacctgga gcagccgacg
     1561 gtagaggtcg tcgtaccggc gggccatgac ctcgctcgag tagtcgttga tcaccttctc
     1621 acgggaacgc ccgccgaggg tgtgccgcaa cgccggatcg tccaccaggc ggcgcagagc
     1681 ggccgcgaag gcgtccgcgt cgccgacggg gaccttgagt ccgtccacct cgtcctgcac
     1741 cagcgactcc atcccactca cggccgtgca gaccagaggt cttcccaggg cggcaccctc
     1801 catgaccgag taggatactc cctcgctgta tgaggtgagc aggttgacgt cgatggcctg
     1861 gtagaagtcg tagatgttct ccacgaagcc gaccatgtga acctggtctg cgaggccgag
     1921 gacacggata tgctccctgt agtccgccat cagggtggga tcgcccgagc cggcgaccac
     1981 gaattgcacc ggttctccct tgaagcggtg cgcgacctcg atcaggaggt cgatgccctt
     2041 catcgggtgt agcctggccg cgagtccaca gatcacctgt ccctcgtccg gcacacagcg
     2101 ctttcggagg aactcctgcc ggctcatggt gggagcgacg agggagagat ccacgccgtt
     2161 cgggatatgc tcgcggcggt cgggggagac gccttcctcg cgcaggagtt ccaggagttc
     2221 gtatgagacc gtcgcgaaac gttcgatgcg cccgaaagcc cagcgaatca gcggcagggc
     2281 cacggccgaa cggtagtggc tgtcggcgta ctcctgacgg aagtcggagt ggatggtggc
     2341 ggccacatgg agcggctccc cgcgcgcggc gatcgccgcc atgaccatgg gcttcaggcc
     2401 gtgcacgtgg atgacccatc cctggtcgtg tttctcgcgg agcgtccgga ccagtcggcg
     2461 tagtgcggcg ggccgcatag cgcattcacg caccgggatg ccgagcctgc gtccgttcgc
     2521 gatcaccttg cccggtccta tcgcgatgag ttccaccgtc ccggacttgc gctgggcccc
     2581 gctcagagcg atgacctggg tcatggcccc tccgatgtca gcgccgttga cgatgtggac
     2641 tatctgcatc ccgattcctc tgggccgctt ccagacatcg accgagcgcc tcggtctgga
     2701 cccgtcgact gtaccgctgg attatagccg cctcgggtgc gaaggacgcg cggccgtcgc
     2761 accaagcccg gtacgaggcc gcgagggcgt ctcgtatgcc cgcgacgtcg gcgaagtcga
     2821 cggcggcgcc ggtgcgcgtc tcgcgcacga cccgcgccgc ctctccctcg aggggcacgg
     2881 tggccacgat tgggatgccc atgcggatgt actcgaaaac cttcccggtg gcgaagttga
     2941 cctcgctctc tatcaagagg aggacgttgg cggcggccag gtgccccatc agcgagccgt
     3001 gggccatata tggggtcacc ttcacgaccg cgtcgagggc gtctccggcg accaggcggc
     3061 ggatgcggtc ctcgtggatg ttcccgacga actcgacctc gagtcgcgcg gggtccacgt
     3121 cggccgaggc gatcaactcg gccagggcct gcagcacgta gtcgggacgc cggcgcccgt
     3181 agagggcgcc ggcgtagacg atgcggaact tctcgttccc ctggttgcgt ggggcgtagt
     3241 cggcgaagtc gtccggatcg aagccgttgg gtatggcggc ggagcggtcg cgcacccccg
     3301 gcgtgtcggc gacgaagttc tccagcatgc gcggcgtgtt cgctaccagg tagtcgcagc
     3361 ccgcgacgac ggccttctcc atccgcctct ctgccgggaa gcgcagcctc ttcagcaact
     3421 ggtcgcggct gtaggggttg ttggtccact cgtcgcggaa gtcggctacc cacgcgacgt
     3481 gggggcggcg tcgcttcagg tagagggcca tcaggtggct gctgtaggga taggaggtgg
     3541 agtagagcag gtcgatgtgc tcggcgtcga gccaggcgag gacgcgttcg cggtgacgta
     3601 ggtaccagaa gacctcggcg tcgggcacca tgagtcgcgt gccgatcagg cggcgcactc
     3661 ggccgatcac tcccgtgccg tccacccagt cgggtcgttc gaggtcgagc cggtggagcc
     3721 cctcgggcag atccttcagc agcgactcgt ctctgagccc gccggagtag gcgcgggtga
     3781 ccacgtaggg cgcgtagccg acggcgggca ggtacttcac gaacttggtg ctgcgctgca
     3841 cgcccgaacc gcctatcggc gggaactcgt tcaagatcat gagtagtttg ttcattgccc
     3901 tcttcgctga ccctgtgtca tcccgatcgc gaaccgggat ctgtgggtag gtgtccgttg
     3961 atcggggccg tcagagtggg cgcccgtgga tcggtgcccg cggatcgggc gctccacgtc
     4021 gcggccggcc aggccacaac gtagcacatc gacgggtgac gactcgttgc ccgcttcttg
     4081 aagcgacgca tacggcgcgt actcccatcg gcaacaggca cgcgggatct cgcagccaac
     4141 cggttgatta agcacccgtt cccggcggtt ctcaggaccg gggcagtctc aagcgacgcg
     4201 acaccttgcg ggggagtccc cgaaggaagt gtttccagtt cgcgtaggag cggacggggg
     4261 ccatgatgcc ggggtcggtt gagcggaatt gggcgtccgc gagcgattca aaatgccaat
     4321 tgagcccccg cttgaagggg atatcctcgt cggacgaacg ccgggttcgg gccaagcatc
     4381 ggtcggggcc gtagaaacga acttcggcgc gattgaaccc catgaactcg cacatgtcgc
     4441 tgatgcagag gccggatggc tgccaccaca ttcccctgta gaagcgcccg aagttggcga
     4501 aggagcgttt gttgtcgttg acggcgttgc tttcaatcag caggagtccg tcgggtttca
     4561 gcaggcgtct caagatgtgc aaccccagca acatgtcgcc gaggtggtac aggacgccgc
     4621 tgaggagaac aaggtcgagg ctgttctccg gaatatgatc gataaggtcg tacactgaag
     4681 ccttcacggc tctcaccgaa gtctgttcga agacttccgc taggaagacg gcctgatcga
     4741 ggtgtgcgcc cacctcatct atggcgtaga caatggatgg tcctctggcc gccatgagga
     4801 gagagagtcc tcccgtgaag caccccacgt ccgccacccg catcccgctg aggtcgtcgg
     4861 gccaccagcc ccactcatcg agcatccccg cgacgtcaag gtagttgtcg ccgagtagac
     4921 cctgcttgcg aaaagggccg aagtcgatgg agtgacccca gccgtactgc gcagccgagt
     4981 ccagggctgc gacgccttct ctgatctcag cgacgcggtc ggggggagtg tcgggcgaca
     5041 gccgaaaggg ccatgccgct gcaggtatct ttctccgggc cacttcttcc tcccactcgc
     5101 tcgccggatc tgagccgaag cgtgcctgca ggctcgccgt ggccgaatcg agaagtcagt
     5161 ctagcatcgc cggcctcgcg cgcggtgatt ctcgcggatc gcgggcggga gtacggacgc
     5221 tactccccgc gcaggtcgcg atggcggcgc tcaagcccgc cttggcgtct aggcctggac
     5281 gcgtgccaga acgcttactt cgtaccgctc tccagcaggt ggcgcggcaa tcacaccggc
     5341 gagtttaccc atcagttcac ggtagagctg gctggattcg aatgccgtca agtcggcctc
     5401 cgtttcccac aggttgatgg agaccgcctt gccggtatcg ctctgtgtga ggagaaactg
     5461 ctccctgaat cccttctgct ccttcaacac cggcacgatg gcatcctgga ctattcggtt
     5521 cgcttcgtcg atcttggcag agtgaaactg gatattgaca gctcgcgcat acatggccga
     5581 cctccctctt cgcgtgcctc gagcatacgg cgaagctttc gagctggcaa gcgagcaagg
     5641 cgtcgatcac gatgggccga cggggccgga agagctgcac caacgtggta gaatgtacag
     5701 gattctgtac atacacctgc gggggtcgtg atgcagcaga ctaccttcac cgacttcaga
     5761 cggcatgcca aggactactt cgacgcggtg gaagggggcg acgtggtgcg ggtgtatcgc
     5821 aatggtcgcc cgattgccga cgtcgtgcct atcccggccg aggaacctgc ctggaagcat
     5881 ccggttgtcc ggatcgcggt gcgtggaccc cgcgcctccg acatggtgat ccaggatcgg
     5941 gcggaggatc cgtcgccgga gtgagggtct tcttcgacgc atcggctctg gtgaaacggt
     6001 atgtcgatga gcctgggagc gacacggtct ccgcgatcct cgaagcggcc gaggtgctca
     6061 tcgtgtcggc gctctgcctg ccggagtgcg tttcggcctt tcagcggatg gcgagtgaag
     6121 gcttgctgtc acaccaggag tacgtctggc tgaaagacgc cgttctcgcc gacttggccg
     6181 atgcgatcgt ttgccatgtg gtgcccgagg tggtcgacgg agccgtcgaa tgcctggagc
     6241 gccaccctgt acgcacgctc gatgcaatac agatcgcatg cgcccgcgcc gtgacggccg
     6301 acctgttcgt caccgcagat cgaaggcagg gtgcggctgc gcgtggggag ggtcttgagg
     6361 tcagggtggt gtagaaagtg gtgtagaaag gaggtgagtc cgggcgagcg ggctctccac
     6421 gtcttccggg tgcatccagt cggccgaaga tctctgagca gcgccgcccc gagctttccc
     6481 gcttgactcc tttccgcaga ggtagtgtgc ggacttgtgt gcagaagtat tccggacggc
     6541 agagggtcat gacgctccca gacagtccgg acttgcattg ccccttcagc gatatctgct
     6601 gggagagaat ggagggtgct gaggtcgcgg ccggccaatt gcggggcccg gagtccggcg
     6661 ttgaccatcg accagtgccc ccgaagggtc tacaagagct gcttgaactc cgcgacggtc
     6721 aatcctgcga tcgacctgtc gcctgacgat ctggctctcc cgctgcggtc cttctcgtac
     6781 cccaccttcg acagcgccgc taccacctca cggccggacg cacgtggcaa ctgagtcaca
     6841 ccgctacctc gataacctct tcgtcgatcg acggcggtat gggctcaccg tgagcctcca
     6901 ggctctcgag ataggccgca gtcgcttctc ggatgttctc catggcctcg gagcgcgtct
     6961 gcccctgtga gatgcatcca ggcagggagg ggacttcgac tacgaacact ccgtccacgc
     7021 cttgctggat gagaacacgg tacttcatgc aggcctccat tggcggctct gccctgggcc
     7081 tcgtgacggt cggaacagta cgcgctcgcc agggtccgaa cgaccaccat ttcattgtac
     7141 acggagcccc gcaaatcagg tggcggaggg ggcgggattc gaacccgcga gggttgtgac
     7201 accctgccgg ttttcaagac cggtgcatta aaccaggctc tgccacccct ccgcgaacca
     7261 cgcggacagc gccccattgt gccgcctggc catgctccgc gtgcgggccc gactctcccg
     7321 acggcgcgaa tccgtccgtc tgagtccccg cgccgtcgat tatatcctgc gggcgagcgt
     7381 cagaagttgt gcgaccagtt gcgccggctg gtcgtcagcc tagaggagtc tctccagctt
     7441 cacgatccac cccgggggga cacgaggtcc agcagcgcgc tccggcggag cggctcggca
     7501 tcgaggtatc gtgggacgac gtattccacc acgcgggcga aggcgtctct gtcgcgtacg
     7561 atcagggtcc gaccgttcag cgcccggaat tgggc
//
LOCUS       DASRMJ010000281         4283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_468871, whole genome shotgun sequence.
ACCESSION   DASRMJ010000281 DASRMJ010000000
VERSION     DASRMJ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468871"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /gene="serS"
                     /locus_tag="VFD74_07765"
     CDS             <1..1160
                     /gene="serS"
                     /locus_tag="VFD74_07765"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZK49479.1"
                     /translation="RDLIVHVEEKRGLRNAVSEEIAAAKKAGEDAADRIAAMREVGAS
                     IKTLESDLKTAEEALERELLQVPNLADPSAPDGGEESSVVTHNWGTPREFEFEPRDHL
                     DLGTESDMIDMERGGKVAGSRFAYLKGDLVLLQFALVQFALQKLMAKGFRPVVPPVLV
                     RDEAMYGTGFFPTDMQQVYQVESDALNLVGTSEVPLAALHMDEILDESDLPVRYVGYS
                     SCFRREAGAAGKDTRGIFRVHQFDKLEMFSFCHPDRSTEEHDFMRGVEEEVLQDLGLP
                     YQALNVAAGDLGASAAKKYDLEAWIPTQGRYREVTSCSNCTDYQARRLRCRAKGKDGS
                     PYLVHTLNGTVVAVGRTIIAIMENYQTADGLIEVPAVLRPYLLPGMTVLGA"
     gene            1192..1833
                     /locus_tag="VFD74_07770"
     CDS             1192..1833
                     /locus_tag="VFD74_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HZK49480.1"
                     /translation="MDVIRPTEALIIVDVQNDFLPGGALAVDEGDQVVAQINRLLPLF
                     AHVVYTRDWHPADHVSFADPPEYRDGSWPAHCVQGTTGAGFPRELKITDRALLVNKGT
                     DPGVETYSGFRSSAVDMAAWLRERHVDRLYVTGLATDYCVQATALDGLAAGFDVMLVE
                     DCVRGVAPDTTRSALEEMRRAGVGSTTAAAISMAVAVAVATGAETEPPLEGEG"
     gene            1840..3312
                     /locus_tag="VFD74_07775"
     CDS             1840..3312
                     /locus_tag="VFD74_07775"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZK49481.1"
                     /translation="MTDAGSRWARREDLALLTDLYELTMLNGYWRRGRERVGASFEYF
                     FRDLPPHTGFAVTAGLEQLLEFVENLRFNDDDLAYLATLSMFDREFLAFLRTFRPTVS
                     IDAAPEGTVVFPYEPVVRIEGPLAEAQFLETAVLNALNYPTLVATKTARIRLAANSDR
                     VMEFGLRRAQGPDGGLSGSRAAYIGGADATSNVLAGKLFGIPVAGTHAHSWVMAFEDE
                     LTAFRTYAELYPESCVLLVDTYDTLASGLPNALTVFRELRESRPGVRAAVRLDSGDLA
                     RLSKAAYAMFTQAGFSDPLIVASNELDEDLIADLKRQNAPINAWGVGTHLITSSSWPA
                     LGGVYKLTAMREADVWEPRIKLSSNPAKMTDPGRKRVVRYRDAEGRPVGDILYRADEE
                     PHGGPLVSYVGRAELGFQMGLGGVAAFEDLLTPVMRGGRRLAPPAPIAAARQRAVEQV
                     ASLPEELRRLRNPEIYHVGISSELAALKADLIRHSPGVLR"
     gene            3368..3829
                     /locus_tag="VFD74_07780"
     CDS             3368..3829
                     /locus_tag="VFD74_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49482.1"
                     /translation="MERHKPKASARTQLLLAEVMWTAVGTMLLTFGLHWVTRRYHGPG
                     WAYAAPFVIVGLLKGRFVLDRVARRTIARIDMRGDSHCVGGFFSPLSWALVVGMMVLG
                     QVLRASPLPRADVGFLYVAVGAALLTSSRKFWKEWFLLRPARAGRPGKGGG"
BASE COUNT          700 a         1444 c         1454 g          685 t
ORIGIN      
        1 gccgagacct catcgtccac gtggaagaga agcgcggcct gaggaacgcg gtgagcgagg
       61 agatcgcggc agcgaagaag gcgggcgagg acgccgccga tcgcatcgcg gcgatgcgcg
      121 aggtgggcgc ctcgatcaag accctcgagt ccgacttgaa gacggcggag gaggctctcg
      181 aacgcgagct tctccaggtt cccaacctgg ccgacccttc tgcccctgac ggcggcgagg
      241 agagcagcgt cgtgacccac aactggggca ccccgcgtga gttcgagttc gagccgcgcg
      301 accacctcga tcttggcacc gagtcggaca tgatcgacat ggagcggggc ggcaaggtcg
      361 cggggagccg cttcgcctac ctgaagggcg acctcgtgct cctgcagttc gcactcgtgc
      421 agttcgcctt gcagaaactc atggccaaag gctttcgacc ggtcgtgccg cccgtcctgg
      481 tgcgcgacga agccatgtac gggaccggat tcttccctac cgatatgcaa caagtatacc
      541 aagttgaatc agatgcactg aatcttgtag gaaccagcga agtgccgctg gccgccctcc
      601 acatggacga gatcctcgac gagagcgacc tgccggtccg ctacgtgggc tattccagct
      661 gcttccgccg ggaagcgggc gccgcgggca aggacacgcg cgggatcttt cgcgtgcacc
      721 agttcgacaa gctggagatg ttcagcttct gccaccccga ccgctccacc gaggagcacg
      781 acttcatgcg cggagtggag gaggaggtgc tccaggacct cggcctcccc taccaggccc
      841 tgaacgtcgc cgctggcgac ctcggagcct ccgcggccaa gaagtacgac ctcgaggctt
      901 ggatccccac acagggacgc taccgtgagg tcacaagctg ctccaactgc accgactacc
      961 aagcccgccg cctcaggtgc cgcgcaaagg gcaaggacgg gagcccctac ctggtgcaca
     1021 ccctgaacgg cacggtggtg gcggtaggcc gcacgatcat cgccatcatg gagaactacc
     1081 agaccgccga cgggctcatc gaggtgccgg cggtgttacg gccgtacctg ctgccgggta
     1141 tgacggtcct tggcgcctga tcccacgggt gcaaccgaaa ggagcccgac catggacgtg
     1201 atccggccta ccgaagctct catcatcgtg gatgtgcaga acgactttct tcccggagga
     1261 gcactggcag tcgatgaggg cgaccaggtg gtggcccaga tcaaccggct gctcccgctg
     1321 ttcgcccacg tggtctacac gcgcgactgg catccggctg accacgtgag cttcgccgac
     1381 ccgcccgaat atcgcgatgg ctcctggccg gcccactgcg tccaagggac cacgggggcg
     1441 ggattccccc gcgagctcaa gatcacagac agggccttgc tggtgaacaa gggcacggac
     1501 ccgggagtgg agacctatag tggcttccgg tcgagcgcgg tggacatggc cgcgtggcta
     1561 cgcgagcgcc acgtggaccg gctctacgtc accggtctcg ccaccgacta ctgcgtgcag
     1621 gcgacggcgc tcgacggttt ggcggccggt ttcgacgtga tgctcgtgga ggattgcgtc
     1681 cggggagtag cccccgacac cacgcgctcc gccctggagg agatgagaag ggcgggcgtg
     1741 gggagcacca ccgcggccgc gatttcgatg gcggtcgccg tggcggtggc caccggggct
     1801 gagacggaac ctccgcttga gggcgagggc tagccgccca tgaccgacgc gggcagtcgc
     1861 tgggcgcgac gggaggatct ggccctcctg accgatctct acgaactcac catgttgaac
     1921 ggctactgga ggcggggccg ggagcgtgtg ggcgcctcgt tcgaatactt cttccgcgat
     1981 ctgccgccgc acaccggttt cgccgtgacg gcgggtctcg agcaactgct cgagttcgtc
     2041 gagaacctcc gcttcaacga cgacgacctg gcctacctcg ccaccctgag catgttcgat
     2101 cgtgagttcc tcgcctttct ccgaaccttc cgacccactg tgtccatcga cgcggcgccg
     2161 gaagggacgg tggtcttccc ctacgagccc gtcgtgcgga tagagggccc tctggcagag
     2221 gctcagttcc tggagaccgc ggtgctcaac gctctcaact accccacgct cgtcgccacc
     2281 aagaccgccc gcatacggct ggccgcgaac agcgaccggg tgatggagtt cggcctgcgg
     2341 cgggcgcagg gaccggacgg tggactgtcg ggctctcggg cggcgtacat cggcggagcg
     2401 gacgccacct cgaacgtcct cgccggcaag ctgttcggca taccggtggc cggcacccac
     2461 gcacactcct gggtgatggc gttcgaggac gagctcacgg ccttccgcac ctacgctgag
     2521 ctgtatccgg agagctgcgt ccttctggtc gacacctacg acactctcgc atccggcctc
     2581 ccgaacgccc tgaccgtctt ccgcgagctg cgcgagtctc gacccggcgt gcgggccgcc
     2641 gtcaggttgg actcgggcga cctcgcacgg ttgagcaagg cggcctacgc catgttcaca
     2701 caggccgggt tctccgaccc gctcatcgtc gcctccaacg agcttgacga agacttgatc
     2761 gccgacttga agcgtcagaa cgcccccatc aacgcctggg gcgtggggac ccacctgatc
     2821 acgtcgtcga gttggcctgc gctcggggga gtctacaaac tgacggccat gcgggaggcc
     2881 gacgtctggg agccgcgcat caagctgagc tccaatccgg ccaagatgac cgacccggga
     2941 cgcaagcggg tggtccgcta ccgcgatgcc gaaggccggc ctgtgggaga catcctctac
     3001 cgcgcggacg aagagccgca cggcggtccg ctggtgtcgt acgtaggccg cgctgagctg
     3061 ggattccaga tgggtctcgg aggcgtcgcc gcgttcgagg atctcctgac accggtgatg
     3121 cgtggcggaa gacggctggc tccccctgcc ccgatcgcgg cggcccggca gcgcgccgtg
     3181 gaacaggtgg cgtcactccc ggaggagctt cgccgccttc gcaaccccga gatctaccac
     3241 gtgggcatct cctccgagtt ggccgcgctc aaggccgatc ttatacgcca ctcgccgggg
     3301 gtgctccgat gagcgcgcgg ctcgttgccg gttcgcggca ggcgccccga tgagcgtccg
     3361 gagctggatg gaacgacaca agcccaaggc gagcgcgcgc acccagcttc ttctcgcgga
     3421 ggtgatgtgg acggcggtgg gcacgatgct gctcaccttc ggccttcact gggtgacgag
     3481 acgctaccac ggcccggggt gggcctacgc ggcccccttc gtgatcgtcg gcctgctcaa
     3541 gggtcgcttc gtcctcgacc gcgtggcgcg ccgcaccatc gcccgcatcg acatgcgcgg
     3601 tgactcacat tgcgtgggtg gcttcttctc tccgctgagc tgggccctgg tggtcgggat
     3661 gatggtgttg ggacaggtgc tccgcgcttc gccgctcccc cgcgccgacg tgggctttct
     3721 ttacgttgct gtgggagcgg cgctgctgac gtccagccgc aagttctgga aggagtggtt
     3781 cctcctgcga ccggcgcgcg ccggtcgccc gggcaaaggc ggaggttgac gccaccgtcc
     3841 gctgacgccg gcggcgggag tcgtcgccgc tggtgagaca ggccacgcgc acggtaccat
     3901 ggcagcgcgg cccaacgata ggtgcgacaa caaggagagc ccggtgttct gtgcgtcatg
     3961 cggagccgat aacccgttcg acgctgcgtt ctgcgctcac tgcggcaggc ccgtgaccgt
     4021 gccgacagcc gaggggcggc cattcgcgtc ggcacggccg gcacaggcgg cacggccggg
     4081 acggccggga cggccggagc cctcgggacg accgccggcg tccgcaggcc cgaagcggtc
     4141 ggggcgaccg gcaccgaccc cacagccttc cggggctggg cggccgccca gccctcgagc
     4201 cgacgtaaag acgatcgtca cgatcgcggt ggcggtgttc gtggggttcg ccggcctcgg
     4261 cctggccgca tacctcttgg tat
//
LOCUS       DASRMJ010000282         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_533861, whole genome shotgun sequence.
ACCESSION   DASRMJ010000282 DASRMJ010000000
VERSION     DASRMJ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533861"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..2728
                     /locus_tag="VFD74_07785"
     CDS             362..2728
                     /locus_tag="VFD74_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZK49483.1"
                     /translation="MIGNYIPRRCGIATFTTDLAESLAAEAPESEVWAAAMNDTPEGY
                     PYPSRVHFEMSESSRGDYLKAAEFLNICGVEVASLQHEYGIFGGPAGEFILDLIGQLR
                     MPVVTTLHTVLREPTRDQMRVSAEIARLSAKLVVMSRRAASFLRDIYQVPAEKIAFIH
                     HGVPDLPFTDPTYYKDQFGVEGRTVAATFGLLSANKGIEHMIAALPEIVEHHPDFMYL
                     VLGATHPNVQKHEGERYRTLLERLAVSLGVAEHVRFLNRFVDLPELCEFLGCADVYVT
                     PYGDEAQITSGTLAYAMASGKAIVSTPYWYATEMLAEERGVITPFGDTEALTANVLAL
                     LSDPVRRHAMRKRAYVFTRDAVWSKVAQEYLNVFAEAKERQQWRPTRGFRARAILPTS
                     TALPEINLAHLRRMSDGAGLLRHATFNLPDRAHGYCTDDNARALVLTVSAQDLLPGDE
                     GLASMSACYLGFLSHALDPGTGRFRNFMSYDRRWEETEGSEDTQGRALWALGVTVAHA
                     PDEGQRAAATSLFHRALSGAEALTHPRASALALVGIHAYLGRFAGDTGAKRVAKLLAR
                     RILHDFKRNATDDWYWFEDILTFDNGKVAQALLLAGTFLKDRTIVEVGVKALEWLTDM
                     QFANGMFRPVGNCGWYRRGGLRARFDQQPIEAQSMIEVYASAYAATGETRWADLADQV
                     FQWYLGQNDLGVPIWDSKTGACCDGLGPEGPNLNQGAESTLAWLLSLTHMHKLRTALS
                     VRSPALLQAADRKNSVSLVIAGRPRSPVARARTGFPAGPPSRVDPVPN"
BASE COUNT          616 a         1058 c         1071 g          594 t
ORIGIN      
        1 gtgttcacgt ccaagagcgg gctgcgcatc taacatctac ccccccagtg agcgacctcg
       61 gataaccgtg aatgtgacgt tcccgtcccg taaccggtct actccaccct cgaggaatcg
      121 ctcttcaaga gggggcggca gcgtatgcgt cgtgggtgtt tgagaaacag actggctgga
      181 aaagatagtc agcacttact cgtcagtcat ggccttgtat tcggaagcca ctccagaatg
      241 accggtgttc gaacgatgcg gtcgagagcc ggcaagtctt gcgcgccaaa cgctcgtggg
      301 taggaacaag agggcaactg tgccggacca gcagctccat tcacgcccca ctcggattgc
      361 agtgatcggc aactacatac cgcggcgctg cgggatcgcc acgttcacga cggacctcgc
      421 cgaatccctg gcagccgagg cgccggagag cgaagtctgg gccgccgcca tgaacgacac
      481 gcccgagggt tatccctacc cgagccgggt gcacttcgaa atgagcgagt ccagccgtgg
      541 ggactacctg aaggcggcgg agttcctcaa catttgcgga gtagaggtcg ccagtctgca
      601 gcacgaatac ggcatcttcg gcgggccggc aggcgagttc atcctcgatc tgatcggcca
      661 gttgcgtatg ccggtcgtga ccacgctcca caccgtactc agggagccta cccgggatca
      721 gatgcgtgtg agcgcggaga tcgcgcggct ctcggccaag ctggtggtga tgagtcgccg
      781 ggcggccagt ttcctgcggg acatctatca ggtaccggca gagaagatcg ctttcatcca
      841 tcatggggtc cccgacctcc ccttcaccga tcccacctac tacaaggacc agttcggggt
      901 ggagggtcgg accgtcgccg ctaccttcgg gctgctctcg gccaacaagg gcatcgagca
      961 catgatcgcg gccctgcctg agatcgtcga gcaccacccg gacttcatgt atttggtgct
     1021 gggcgcgacc caccccaacg tgcagaaaca cgagggcgag cgctatcgga ctttgcttga
     1081 gcgtctggcg gtgagcttgg gcgtggcgga gcatgtccgc ttcctcaatc ggttcgtcga
     1141 tctgcccgag ctgtgcgagt tcctcggttg tgccgatgtg tacgtcaccc cctacgggga
     1201 tgaagcgcag atcacgtcgg gcaccctggc gtacgcgatg gccagcggca aggcgatcgt
     1261 ttccactccc tactggtatg ccaccgagat gctggccgag gagcgggggg tgatcacacc
     1321 tttcggcgac accgaagcac tgaccgccaa cgttctcgct cttctgtctg atcccgtgcg
     1381 tcgtcatgcc atgcgaaaac gggcctatgt gttcacccgg gacgcagtct ggagcaaggt
     1441 ggcgcaggag tacttgaacg tgtttgcgga agccaaggag cgccagcaat ggcggccgac
     1501 gcgggggttc cgggcgcgag caatcctccc cacgtccacg gccctgcctg agatcaatct
     1561 cgcgcacctg cggcggatga gcgacggtgc cggcctcttg cggcacgcca cgttcaacct
     1621 acccgaccgc gcgcacgggt actgcacgga cgacaacgca agagccctgg tccttacggt
     1681 gtccgctcaa gacctcctgc ccggagacga ggggctcgcg tcgatgagtg cctgctacct
     1741 cggcttcttg agtcacgcgc tcgacccggg tacggggcgc ttccgcaact tcatgtccta
     1801 cgaccggaga tgggaggaga ccgaaggctc ggaagacacc caggggcgcg ccttgtgggc
     1861 cctcggcgtc accgtggcac atgctcccga cgaagggcag cgggcggcgg ccacctctct
     1921 gttccatcgg gcgctttccg gcgcggaggc gcttacgcac ccgcgcgcgt cggctctcgc
     1981 gctcgtcggc atacacgcct acttagggag gttcgccgga gacacaggcg cgaagcgcgt
     2041 ggccaaactc ctggcgcggc gcatcctcca cgacttcaag cggaacgcca ccgacgactg
     2101 gtactggttt gaggacatcc ttaccttcga caacgggaag gtcgcccaag ccctgctcct
     2161 tgccggtaca tttctcaagg acaggacgat cgtggaggtg ggcgtcaagg ctctcgaatg
     2221 gcttaccgac atgcagttcg ctaacggcat gttccgaccc gtgggtaact gtggctggta
     2281 ccgaagaggc gggctccggg cgcgcttcga ccagcagccg atagaggcgc agtcgatgat
     2341 cgaagtgtac gcttcggcct acgcggcgac cggagagact cgttgggccg acctagccga
     2401 ccaagtgttc cagtggtacc tcggccagaa cgacctcggc gttcccatat gggactcgaa
     2461 gaccggggca tgctgcgacg gccttggacc ggagggcccc aatctcaacc aaggggcgga
     2521 atccaccctg gcctggcttc tctcactaac gcacatgcac aagcttcgca ccgcgctctc
     2581 cgttcgctct ccggcgctgc ttcaagcggc ggaccggaag aactcggtct cgcttgtgat
     2641 agcaggtcgg cccagaagcc ctgtggcccg agcgcgaacg ggttttccgg ctgggccgcc
     2701 ctcccgggtg gacccggttc cgaactgagg gcgaaggaca gagccggaat gcccccgtga
     2761 cgccttgcgg ctgggttcgg acgccggtaa ttgcgtgtac gaacgagaaa tccgggggct
     2821 ggttcttaga cccgacagcg caacacgaat acgtcagggg tgaggggtac atctattccg
     2881 attcacgctg ctaagcgccg gtagtcgtga accaacccgc cgagccggtc tacccggact
     2941 atctccccgt cggcgcagtt caggatgggc ctgggctccg gggtgtgaag ggatagcccc
     3001 cgatgcggac gctcctgggt gtagtgactg acgtactccc tgaggatttt ccggagatgg
     3061 cccccgccga gcaccaggac atggtccaca cactctcgcc gtgccgttct taggaagcgc
     3121 tcggcacaag cgttcgcccg gggtgagtgg atcgggctgc gaatgacacg aacacacctc
     3181 cccagtgaca cacctcatcg aaagcccgcg agtacgtggc gtcgcggtcc cggatcagga
     3241 actgggcttt ggcgaggcga tcttcactcg cgagggtgcg ggcctgctgg gtaacccaga
     3301 cggaatgtgg atgggcggtg acgcctgcca ggtggaccc
//
LOCUS       DASRMJ010000283         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_533875, whole genome shotgun sequence.
ACCESSION   DASRMJ010000283 DASRMJ010000000
VERSION     DASRMJ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533875"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1174
                     /gene="miaA"
                     /locus_tag="VFD74_07790"
     CDS             56..1174
                     /gene="miaA"
                     /locus_tag="VFD74_07790"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZK49484.1"
                     /translation="MSATEGPHNRPNDLARSLLRHPLGGEAADGGLVIAILGPTGVGK
                     TEVAARVALELGVRVISCDSMQIYRGFPVLTNQPGEAGAAGSVRHELVAVAEPQEEWS
                     AAAYARRAQALIDEDIAATGWAVISGGTGLYMRAALAPLAMARPCDPERRAALERLAA
                     NEGPERMHERLAAAAPEVAEKIHPHNVRRVVRALEVIEAGGAEGWSERRDLWRPEYRH
                     PTLVVVLAVDRTVLYERIERRAREMVRSGAVEEVERHRALQSRAGAPPPSGSGGPAEV
                     ASTLGAPASPPRGVERAIGYRELAAYLDGRISRDEAIASLAAATRRYARRQVTWVRKL
                     GDADIIDTSERSPQETSAEVLQEAYALRRIPPTEPDVG"
     gene            1179..2084
                     /gene="dapF"
                     /locus_tag="VFD74_07795"
     CDS             1179..2084
                     /gene="dapF"
                     /locus_tag="VFD74_07795"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZK49485.1"
                     /translation="MRFSKWHGLGNDYIIVEKTDLPGIPTSDQCALLCDRHFGVGGDG
                     ILVHCPPTGAVPGAVARMRIFNPDGSEPEMCGNGIRMFALYLQRRDLIGDGEAIIETL
                     AGPIRSTVLDDGRVRVHMGTARFNSPSIDSVPWGPDAEIVERELEADGVAYNFTFVDV
                     GNPHCIIVCDSLEAVDLERVGPSIENHPLFPHRTNVEFIQWRRDDSVSMRVWERGVGE
                     TQACGTGATAVGAAAVRLGLAGSPVTVHLLGGDLEIEAADDGQVVMIGTADEVFTGRL
                     SRDLLTRLDWAGDDSSRPPTTTRSK"
BASE COUNT          339 a          671 c          750 g          324 t
ORIGIN      
        1 gggtgtggcc gtcgagcgcc ccgcatgcgg gtgaggggcg agtgcggagc ccggcgtgtc
       61 ggccactgaa ggcccccaca accgcccgaa cgaccttgcc cgcagcctgc tgcggcaccc
      121 acttgggggc gaggccgctg acggcggcct ggtgatagcc atcctcggtc ccaccggagt
      181 cggcaagacc gaagtcgcag cgcgtgtcgc ccttgagttg ggagtcaggg tgatctcctg
      241 cgactcgatg cagatctatc gtggcttccc ggtgctcacg aaccagcccg gcgaggccgg
      301 ggccgcgggg agcgtgcgcc acgaactcgt ggccgtggcg gagcctcagg aggagtggag
      361 cgcggcggcg tacgcgcgtc gggcccaggc gctcatcgac gaagacatcg cggctacggg
      421 atgggctgtg atctccggcg gcacggggct ctacatgcgc gccgccttgg cgccgctggc
      481 tatggcgcgg ccttgcgacc ctgaacgccg cgccgccctg gaacgcctgg ccgccaacga
      541 aggtccggaa cggatgcacg agcgcttggc cgctgcggct ccggaggtcg ccgagaagat
      601 ccatccccac aacgtgcgac gggtcgtgcg tgccttggag gtgatcgagg ccggaggagc
      661 ggaaggttgg tcggagcgcc gcgatctatg gcgacccgag taccgtcacc cgactctggt
      721 ggtggtcttg gcagtcgacc ggaccgtgct ctacgagcgc atcgagcgcc gggcgcggga
      781 gatggtgcgg agcggcgccg tggaagaggt ggagcggcat cgagcactcc agtcgcgcgc
      841 gggggcgccg ccaccctcgg gatcaggcgg gccggcagag gtcgcgtcca cgctaggagc
      901 gcccgcatcg ccgccgcggg gcgtcgagcg cgccatcggc taccgagagt tggccgcata
      961 ccttgacggc cggatctcgc gcgacgaagc catcgcctca ctcgctgccg cgacgcgccg
     1021 ctacgcccgg cggcaggtga cgtgggtgcg caagctcggt gacgctgata tcattgacac
     1081 ttccgagcga agccctcagg agacctccgc cgaggtgctg caggaggcct acgctctccg
     1141 caggattccg ccaaccgagc cggatgtggg gtaggagcat gcgcttcagc aagtggcacg
     1201 gcctcgggaa cgactacatc atcgttgaaa agacggatct gcccggcatt cccacttctg
     1261 accagtgcgc gctgttgtgc gatcggcact tcggcgtggg tggagatggc atcctcgtcc
     1321 actgtccgcc caccggggcg gtgcctggtg ccgtcgccag gatgcgcatc ttcaatcccg
     1381 acggcagcga accggagatg tgcggcaacg gcatcaggat gttcgcgctg tatctgcaga
     1441 ggcgcgacct catcggggac ggcgaggcga tcatcgagac cctcgccgga cccatccgat
     1501 cgacagtgtt ggacgacggc agagtgcggg ttcacatggg caccgcgcgg ttcaacagcc
     1561 cgtcaatcga ctccgtcccg tggggtccgg acgccgagat cgtcgagcgc gaactggaag
     1621 ccgatggagt cgcctacaac ttcactttcg tagacgtggg aaacccgcac tgcatcatag
     1681 tctgcgactc gcttgaggcc gttgacctcg agcgcgtcgg cccttccatc gagaaccacc
     1741 cgctctttcc gcatcgcacc aacgtggagt tcatccagtg gcggcgggac gactccgtct
     1801 cgatgcgcgt gtgggagcgc ggggtggggg agacccaagc ctgcggcacg ggcgcgacgg
     1861 ccgtgggagc ggcggcggtt cgtttgggcc ttgccggcag tccggtcacc gtccacctgc
     1921 ttgggggtga cctggagatc gaggcggctg acgatggcca ggtagtcatg atcggcacgg
     1981 ccgacgaagt gttcacgggc cggctcagtc gagacctgtt gaccagacta gattgggcgg
     2041 gagacgactc ttcgcgccct ccgaccacca caaggagtaa gtag
//
LOCUS       DASRMJ010000284         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_533917, whole genome shotgun sequence.
ACCESSION   DASRMJ010000284 DASRMJ010000000
VERSION     DASRMJ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533917"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..779)
                     /gene="hisF"
                     /locus_tag="VFD74_07800"
     CDS             complement(15..779)
                     /gene="hisF"
                     /locus_tag="VFD74_07800"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZK49486.1"
                     /translation="MHLKRVIPCLDVDKGRVVKGTNFVDIRDAGDPVELAAFYDREGA
                     DELVFLDITASHERRDTIVELARRCAEELFIPFTIGGGIGSEDDVRALLGAGADKVSL
                     NSAVLRDPTLIERCSRRFGSQCVVVAIDAKRVAGDAPRWEVFLNGGRLTTRRDAVEWA
                     REAVRRGAGEILLTSMDRDGTKDGYDLELTRAVAQAVEVPVIASGGAGTLDHLAQVIL
                     ETGADAVLAASIFHYGEFTVRQAKERMAAAGVPVRL"
     gene            complement(781..1518)
                     /gene="hisA"
                     /locus_tag="VFD74_07805"
     CDS             complement(781..1518)
                     /gene="hisA"
                     /locus_tag="VFD74_07805"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZK49487.1"
                     /translation="MRLFPAIDIQGGRCVRLRRGDFSDETIFSHDPVEVARRWVESGA
                     RSLHVVDLDGAREGDMRNLPLVAAIAEAVTVPVQFGGGIRSEAALDRLRGTAVNRIIV
                     GTGALLDEGFLRAALADWGSRLIVAVDAEDGFVSTHGWRQRSGMSASSFVRQLAESGI
                     REVIYTDIDRDGMLGGMNLKAIEELVRSVPEMEVIASGGLTSLQDLRALKRLEPVGVT
                     GVIAGRALYEKIFTLQQALDVLDGIEP"
     gene            complement(1577..2188)
                     /gene="hisH"
                     /locus_tag="VFD74_07810"
     CDS             complement(1577..2188)
                     /gene="hisH"
                     /locus_tag="VFD74_07810"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZK49488.1"
                     /translation="MLYVVDYGSGNLRSVQKAFEYVGAQAEVGCDVRRIRDAGGLVLP
                     GVGAFGAAMAELEGRGLAEVLRERIEAGVPFLGICLGLQLLFESSDESPGVEGLGVIR
                     GQVRRLPPGPKIPHIGWNQVEIVKESDLLEGVPDMSAFYFVHSYVVVPTRPADVLTIT
                     EYGSTFVSGVERENVTAFQFHPEKSSALGLRVYENFARRVRRD"
     gene            complement(2194..2799)
                     /gene="hisB"
                     /locus_tag="VFD74_07815"
     CDS             complement(2194..2799)
                     /gene="hisB"
                     /locus_tag="VFD74_07815"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZK49489.1"
                     /translation="MTNTSGESRTAKVERRTRETEVSVSLGLDGGGVCEVSTGIGFLD
                     HMLDLFAAHGRFDLRVQATGDLQTGGHHTVEDVGICLGSALAQALGDNRGITRYGSVQ
                     LPMDEALALVSLDISGRPYLAYNVDLSHANIGGFDLDLALEFFQALVNNARITLHIRL
                     LAGSNPHHIIEAVFKGFGRALDQATRLDPRVHDVPSTKGVL"
     gene            complement(2903..>3551)
                     /locus_tag="VFD74_07820"
     CDS             complement(2903..>3551)
                     /locus_tag="VFD74_07820"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZK49490.1"
                     /translation="SEVVVVADAACDPRLVAVDMLAQEEHGSGASAVLVAPSLEVCRA
                     VTAEIESLRRASAAFVSLPGEDERTGDRDGLFAYYPVPGESFEELAALLVEAYAPEHL
                     ELHLDDARGFLAGVRSAGAVFLGGHTPTAFGDYVAGSNHVLPTGGAARFSSPLSVDTF
                     VRRSSVVEVPAGAAGALTVHLARIARSEGFEFHRLSAEMRVPRAPDAGGEGPQPL"
BASE COUNT          628 a         1260 c         1106 g          557 t
ORIGIN      
        1 caaagccccc ggtcctagag acggaccggc acgccggcgg cggccatccg ttccttggcc
       61 tgccgcacgg tgaactcacc gtagtggaag atcgacgcgg ccagcaccgc gtcggctccg
      121 gtctcaagga tgacctgggc caggtggtcg agggtcccgg cgcccccgct ggcgatgacg
      181 ggcacctcca ccgcctgcgc gacggcacga gtgagctcca ggtcgtagcc gtccttggtg
      241 ccgtcgcggt ccatggaagt gagcagtatc tcccccgctc cccgacgaac cgcctcgcgc
      301 gcccactcga cggcgtcccg cctagtggtc agacgaccgc cgttgaggaa cacctcccac
      361 cgcggcgcat ccccggccac gcgcttcgcg tcgatcgcca ccaccacgca ctgcgaaccg
      421 aaccgtcggg agcatctctc gatgagagtc ggatcgcgca acaccgccga gttcagcgac
      481 accttgtcgg cgcccgctcc cagtagggcg cgaacgtcgt cctcgctccc gatgcccccg
      541 ccgatggtga agggtatgaa gagctcctcc gcgcagcggc gggccaactc gacgatggtg
      601 tcccggcgct cgtgcgacgc tgtgatgtcc aagaacacga gttcatccgc gccttcgcga
      661 tcgtagaagg cggcaagctc caccggatcg cccgcgtcac ggatgtcgac gaagttggtc
      721 cccttcacca ccctcccctt gtccacgtcg agacaaggga tcacacgctt caggtgcacg
      781 ctagggctcg atcccgtcaa gtacgtccaa agcctgctga agggtgaaga tcttctcgta
      841 cagggcccga ccggcgatca cccctgtgac gccgacaggc tcgagccgct tcagggcccg
      901 cagatcctgc aacgaggtga gtccgccgct cgctatgact tccatctcgg ggaccgaccg
      961 caccagctcc tcgatcgcct tcagattcat gccgcctagc atgccgtcac ggtcgatgtc
     1021 ggtgtagatg acctccctga tgcccgactc tgccaattgc cgcacgaagc tcgatgcgga
     1081 catcccgctg cgctggcgcc acccgtgcgt gctcacgaag ccgtcctcgg cgtccaccgc
     1141 cacgatcaac cttgaacccc agtcggccag cgcggctcgc aggaacccct cgtcaaggag
     1201 agcgcccgtg cccacgatga tgcggttcac cgcggtgccc ctcagccggt cgagggcggc
     1261 ctccgaacgg atgccgccgc cgaattggac cggcaccgtg actgcttcgg ctatcgccgc
     1321 cactagtggg aggttgcgca tgtcgccctc acgcgcgccg tcgagatcga cgacgtgcaa
     1381 cgagcgcgcg ccggactcca cccatctccg ggccacctcc acgggatcgt ggctgaagat
     1441 ggtctcgtcg gagaagtcac cgcgccgaag gcgcacacag cgcccgccct gtatgtcgat
     1501 ggccgggaag agtcgcatct gttcctccta cctccgccgg ctcacgagcc cggggaggac
     1561 attcatcggt ctcacgtcag tctcgtcgca ctcggcgggc gaagttctcg tatacccgca
     1621 ggcccaaggc gctggacttc tccggatgga actggaaggc cgtgacgttc tcacgttcca
     1681 cgccggagac gaaggtgctt ccgtactcgg tgatggtgag cacgtccgcg ggcctcgtag
     1741 gcacgaccac gtaactatgg acgaagtaga aggccgacat gtcgggaacg ccctccagaa
     1801 gatcggactc cttcacgatc tccacctggt tccagccgat gtgcgggatc ttcggacccg
     1861 gcggcaggcg ccggacctgc cctcggatca cgccgagacc ctcgacaccc ggactctcgt
     1921 cactgctctc gaaaagcagc tgcagcccta gacatatccc aagaaacggg acccctgctt
     1981 cgatccgctc gcgcaggacc tcggcaaggc cgcggccctc caactcggcc atcgccgccc
     2041 cgaaggctcc tacaccgggt agaacgagac cgccggcgtc gcggatgcgc cggacgtcgc
     2101 aacccacttc cgcttgagca cccacgtact cgaacgcctt ctgcaccgaa cgcaggttgc
     2161 cggatccata gtccaccaca tagagcacgg ggactacagt acccccttcg tcgaaggcac
     2221 gtcgtgtacc cgcgggtcga gtctggtcgc ctggtcgaga gcccttccga atcccttgaa
     2281 caccgcttcg atgatgtggt gagggttcga tccagccaag agacggatgt gcagcgttat
     2341 gcgggcgttg ttgaccagag cctgaaagaa ctcgagtgcg aggtcgaggt cgaagcctcc
     2401 gatattggcg tgcgagaggt cgacgttgta ggcgaggtag gggcgcccgc tgatgtcgag
     2461 gctgaccaag gcgagggcct cgtccatagg gagctggacg ctcccgtaac gcgtgatgcc
     2521 gcggttatct cccaaggctt gagctagggc ggatccgagg cagatgccca cgtcttccac
     2581 cgtgtggtgc ccgccggtct gaaggtcgcc ggtggcctgc acccgcaggt cgaaacgtcc
     2641 gtgggcggcg aagagatcga gcatgtggtc gaggaacccg atcccggtgg agacctcaca
     2701 cacgccccca ccatcgagcc ccagactgac cgagacctcg gtctcacggg tgcggcgttc
     2761 gaccttggcc gtgcggctct cacccgatgt gttcgtcacg attacctccc ccgtccctgg
     2821 cccggtttca ccctgaagtc ggaccgtggt tggaggatag cataccgtct cgcgcgctcg
     2881 gggagggtgg ccgacgcggc actcagagag gctgcggccc ctcgcctccc gcgtcgggtg
     2941 ctcgggggac acgcatttcg gccgagaggc ggtggaactc gaaaccctcg ctgcgggcga
     3001 tgcgcgccaa gtgcacggtc agcgcgccgg ccgcgccggc cggcacctcc acgaccgacg
     3061 atcggcgcac gaaggtgtcc acggagagcg gcgacgagaa gcgcgccgcg ccgccggtgg
     3121 gcagcacatg gttcgaaccc gccacgtagt caccgaaagc ggtgggagtg tgaccgccca
     3181 ggaagaccgc gccggccgat cgcacgccag ccaggaagcc acgcgcatcg tccaagtgga
     3241 gttcgagatg ctcgggcgcg tacgcttcca ccagcaacgc cgccaactcc tcgaacgact
     3301 cccccggcac cggataatag gcgaacaggc cgtctcgatc gcctgttcgc tcgtcttcgc
     3361 cggggagaga gacaaatgcc gcggaagccc ggcggagcga ctcgatctcg gcggtgacgg
     3421 cgcggcacac ctccaaggaa ggagccacca gcaccgccga cgctccgctg ccgtgttcct
     3481 cctgtgccag catgtccacc gccacgagtc gggggtcgca ggcggcgtcg gccaccacca
     3541 ccacttcact c
//
LOCUS       DASRMJ010000285        20116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_243637, whole genome shotgun sequence.
ACCESSION   DASRMJ010000285 DASRMJ010000000
VERSION     DASRMJ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 20116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20116
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243637"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFD74_07825"
     CDS             <1..519
                     /locus_tag="VFD74_07825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZK49491.1"
                     /translation="DRGEGTTLLESGITLPVVEIGLLEEPGADVVGPTDHEILSSLEV
                     RMHLPEGGVPKEGPSAGLAVAVSLLSALTLIPLLPRVALSGEITLTGQVLAVGGLKEK
                     LLAALREGVRKVILPADVRPQIQEVPAELTRGLEIVYVSEFKDALAHVLCVECAASAD
                     GESTPPRRPRNG"
     gene            532..3243
                     /locus_tag="VFD74_07830"
     CDS             532..3243
                     /locus_tag="VFD74_07830"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZK49492.1"
                     /translation="MAEVTMPARYSPHEVEERLMREWLDRGAFRAASEPSGPTYSIVI
                     PPPNVTGSLHMGHALNGTIQDVLTRYHRLRGESALWLVGTDHAGIATQNKVEGRLAAE
                     GLRKEDIGREAFDRRVWEWREQYGSTIINQLKRLGCSCDYDRERFTMDDAYHAAVLKV
                     FVDLYEKGDIYRDVYLVNWCVRCGSAISDLEVEHVERADRLYYVRYPVVGADDFLTVA
                     TTRPETMFGDTAVAVNPDDPRYGRYVGMTAIVPLVGREVPIIADEHVDPAFGTGALKI
                     TPGHDPNDAEIARRHGLPGISAIGFDGLLNEEAGEFAGRDAEEARAAIVSRLTDHGLF
                     ERAEEYTHSVGTCYRCGTVIQPLLSLQWFMDMKRLAAPAIECVEDGRVGFVPDRWADV
                     YLEWMRGIRPWCLSRQLWWGHRLPVWYCDDCSKVVVAVEAPTSCPDCGGTLRRDEDVL
                     DTWFSSAMWPFATLGWPESTPELKSYYPTSALSTARDILYLWVARMIMMGLEYLDDVP
                     FRSVIVHPTILAADGRRMSKSLGTGIDPLELIDEYGADATRFGLLYMSSVQDVRFSAE
                     RIEMGRNFANKLWNASRLVLQGAHPEAVPAIELATPADRWIFSRLGVVSVELAQLYES
                     FDFAEAARLGYRFVWNEVCDWYLEMAKSRLYGDSEAERLQVSGNLLALLERITGLLHP
                     MMPFVTAEVYRNLPRASPGEGHQGAAGAGAVGADEDAVDLLSSRFPEGDPAWADADAE
                     SGMESFIAVVAGLRSAREELGVPRGAVGRVWIVTSDPGRASALTGEPGALRQLAGCEI
                     AGVVCDEGDVPAGRFTSVDAPGLKVLLPLEGLVDVEKERARLMGRAEKAKADVARASG
                     KLANERFVVNAPAEVVAQEQERLAAGRAQLADIARQYRERLGEELPS"
     gene            3240..4541
                     /locus_tag="VFD74_07835"
     CDS             3240..4541
                     /locus_tag="VFD74_07835"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZK49493.1"
                     /translation="MSGTVSVDQAREYVHGLEMFGMRLGLDRVRALAERLGDPQKAYR
                     TVHVVGTNGKSSTARFISAILQQHGLRVGTYVSPHLVSLAERQLVDGVASTDEEFYSL
                     VARIMPVAAEVEAELPKGEVLTQFEVLTAAAYLYFKESGCDVAVVEAGLGGRWDATSI
                     IESDVQVITTIALEHTEYLGNTALAILEEKAAVIPEGGAVVAGFLDGDVSARLRTICA
                     ERGASLCSLGEEISLLTDIGQATFDVFGARDLYSNLRLSVLGNYQRANAALAIAAAEQ
                     FMGAALDGAKTRKGLLHTSVPGRLEVISDQPFCVLDGAHNPSGMAEMLRSLDGLLARR
                     RLLAVVSILKDKGALEMLADLAPRCDIVFVTQNSNPRSYPAEELAAILEGLDDAPEVF
                     VDADPESAVRSALKLATSNQVVIVTGSLYLVSDLKRSMQGG"
     gene            4674..5168
                     /locus_tag="VFD74_07840"
     CDS             4674..5168
                     /locus_tag="VFD74_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK49494.1"
                     /translation="MDNAFQTIQNILSWPGWTLVWYLIWFSVVVLWLALVFWTLKDAR
                     KRIDDGLITAVAVATSLVFPFIGTLVYSILRPPEYLADVRERELEIHAMEQELMESRV
                     CPNCREPVREGYIVCPKCRKGLRILCVTCERPLEKSWKVCPYCGADAGPVAKVAPVAE
                     VANG"
     gene            5272..5694
                     /gene="ndk"
                     /locus_tag="VFD74_07845"
     CDS             5272..5694
                     /gene="ndk"
                     /locus_tag="VFD74_07845"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZK49495.1"
                     /translation="MSVERTLVLVKPNGVTRGLVGEVIGRLECRGFVLAGLKLVQVTK
                     ELAEEHYEEHSEKPFFEGLVSLLTSAPVVAMAVEGVSAVQVVRDMMGATDPTKAAPGT
                     IRGDFALEIGENVVHGSDSVESARREIALYFTPEELLS"
     gene            5744..6379
                     /locus_tag="VFD74_07850"
     CDS             5744..6379
                     /locus_tag="VFD74_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HZK49496.1"
                     /translation="MPLPTYPEDFRLLLASSSPRRRDLLIAAGIPFETVSTDAVEEDI
                     GTDPRRLAEQNALRKARGARLPAGLHTKVFVLGADTVVVVGGRILGKPGDAGEAGDML
                     RRLSAVEHHVVTGVALIELGVDGEVARELVGASVTAVRFRSLSVGEIDAYLLSEEWRG
                     KAGAYAIQGLASLFAEGIEGDHSNIVGLPLSLLGELLREAGFDPVRRVWRR"
     gene            6516..7541
                     /locus_tag="VFD74_07855"
     CDS             6516..7541
                     /locus_tag="VFD74_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZK49497.1"
                     /translation="MTGFGGRDMAVDLGTANTLVYVRGRGIVLSEPSVVAIDSRTGEV
                     HAVGAEAKRMLGRTPGTITAIRPLKDGVIADFDVTEQMLRHFIQKVHQNRWAHPRVVV
                     CVPSGVTGVERRAVEEATITAGARAVYLIEEPMAAAIGAGLPVSEPTGSMIVDIGGGT
                     SEVAIISLGGIVTAQSVRIGGDELDEAIIAYVKKEYKLMIGGQTAEELKLEIGSAYPI
                     GEDEHAEIRGRDLVTGLPKTLVISSEEVRDAIAEPVTAIVDAIKATLDKTPPELASDV
                     MDRGIMLAGGGSLLKGLDERIRQETQMPVHLAESPLTCVAVGSGRSLEEFEALHRSSQ
                     RTRRRRY"
     gene            7560..8486
                     /gene="mreC"
                     /locus_tag="VFD74_07860"
     CDS             7560..8486
                     /gene="mreC"
                     /locus_tag="VFD74_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZK49498.1"
                     /translation="MQRSAALRRRIVFAVLIVISLTLLTAYFREPAAGLFHVVQNDAS
                     DTLSPVQSLAARAVQPFRDGYRWAGAVLGANARNEQLNAQLESLRGELVLLNEASQEN
                     ARLKELLDFRDAGIFPAGSTFEVARVIGKSPTTWEAWVQIDKGSDDGIALNQPVVGVT
                     LPADRSLSGKGLVGKITAVGKRSARVQLMVDQAFNVAALVQGTRAQGIVTGSLSGRLV
                     MDFVERDQPVEEKLIVISSGFGQIFPKGIPVGIVQSVGEVDVNIYKQIEVRPFVDFDT
                     LEEVMIITTPTAQVEGYEEMSTLVPHEAEGTK"
     gene            8496..9023
                     /gene="mreD"
                     /locus_tag="VFD74_07865"
     CDS             8496..9023
                     /gene="mreD"
                     /locus_tag="VFD74_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZK49499.1"
                     /translation="MVRARLIDVGRLALLLVLAVILQTLAASQLSFLGVTADVFVIVV
                     VLVAVGSGSFTGLVFGFVAGLTADVVFLDPIGMRTFMYLLIGYWAGRYVEEFGLSSAW
                     VVVLLAGTVSLGAQTVYGILQMITGTEGSLLTMVVTQVLPAAMFDGLLSAPLYLGLTR
                     VRLLPQQRAAGPMFG"
     gene            9030..10919
                     /gene="mrdA"
                     /locus_tag="VFD74_07870"
     CDS             9030..10919
                     /gene="mrdA"
                     /locus_tag="VFD74_07870"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZK49500.1"
                     /translation="MYLEPVRRRRLPGEDRSAGMRLAILATAVLLLFAVLVFRLWFLQ
                     ILSGDQFAAQADNQRLRTVLIQAPRGVIYDRNGSLLVTNRAGLSVGILPMDLRSPETV
                     IPHLAAVLGIPAEDIRAKLDTAGQDEYRVVTIRQDVPENPVVSYLLEHSLEFPGVRVE
                     KSYLREYPNKILATHILGYVGEASYEDLQKEQFQTLKGGAVIGKDGVEAVHDSMLRGV
                     VGTRTVEVDAAGVPKRVIQENQPTPGSNLVLTIDSGLQAVARAAVVDGIARAHADGFV
                     NSYAGVIVALDPRSGEILAMTSYPDYDPSLWVGGMSTETYQNLIDPAQHNPLFDRATK
                     GLYPAASTFKPFVAATALRSDLIDWTQIFSDPGTFTRDQQVWRDWLLQGHGDVNLVQA
                     LAESCDVYFYNLGSRFYDLKGPVLQDGLREFGFGQRTGIDLPGEYPGQVPDSEWKKKN
                     GKTEIDRIWKPGDDINLSIGQGDLLVTPLQLAIALAAIANGGDILVPRVARQITDASG
                     NVTKQFETEVKSHVDIGAEDLDAIRRGLKMVTSDPTGTAYGAFRGFPIAVAGKTGTAQ
                     KKPGDDYAWFMGYAPADAPEILVVALIEQGGHGSSIAAPAVRKVLEAYFRTESSGPAQ
                     VEVTE"
     gene            10964..12133
                     /gene="rodA"
                     /locus_tag="VFD74_07875"
     CDS             10964..12133
                     /gene="rodA"
                     /locus_tag="VFD74_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZK49501.1"
                     /translation="MSRYLGSLDWVLLAATAGLVCFGLAMIYSATHADLTASSPTQYV
                     WAQSLGLMIGVIALVVLSLIDFTWFARLGRYLYWMNIGLLALTLVIGSERMGARRWIS
                     LPFFDVQTSEIGKVLLVLALAAFLAEGVELRGSLRFVLKAVIYVAFPATLIFLQPDLG
                     TALVFVAIFVCMVLVWSIRWSHLALLAGAAVAAATLVLRVLPRTFGIELLRPYQLQRL
                     TVFVNPGQDTSDAAYQLSQSKIAVASGTFTGKGFMQGTQTHLNFLPAHHTDFIFSVIA
                     EELGFVGSLVLLLLFAVVLWRAFRIASLSKTLHGTLIATGAIGVLVFQVFVNVGMTLG
                     IMPITGLPLPFISFGSNSLMVFLILVGLLQSVHVHSRTALYGGRLKGDPYGQMAA"
     gene            12114..12965
                     /locus_tag="VFD74_07880"
     CDS             12114..12965
                     /locus_tag="VFD74_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49502.1"
                     /translation="MAKWPLDPNKIWGAFKEIAAATAQDAGIVLAGDPSLTAKAQEAL
                     SPDGWHARLWQGAAEGLAGLALGPGDVLLVLVQPNEEDVWAAAVRAAALPLGAVVVVD
                     DGPAATQRTTWYDKHRARVSFSPTPEGWTAVWTAVVEVGGDHVVALGRRFPILRGWAS
                     RKVIKRTARQNGLVGAAFIVPGTDMPIMTLNQIKMVLSIAAIHGEEITTERAIELVGV
                     VGSGFGMRAVARQALDLVPGPGWLFKGAVGYTGTRALGEAALRYFDLGAPATPGRLSS
                     LARRIKR"
     gene            12962..15547
                     /locus_tag="VFD74_07885"
     CDS             12962..15547
                     /locus_tag="VFD74_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HZK49503.1"
                     /translation="MTRVAVSPDLGGIHRRFEGILGRVQNPARLVGGEIGAGPGFSGR
                     TGELRVALAFPDAYEVGISNQALQILYHVAGTVTGVGVERAYLPWVDAIAEMRREGVP
                     LLTLETWTPVKAVHLLGITLQHELNYTNVLELLDLAGIPVRTADRGEDAPLVVAGGPA
                     TADFLPMAPFLDAVVVGDGEEVFAELLASLVEAGAAGEGRAERVRRLAGIRGVFVPGS
                     SRIVRRVALDRLEDAPYPEHCLVPLTAGVHDRAWVEVARGCSRGCRFCQAGMWYRPVR
                     ERSRDRIVELAGSQLRASGHQEVSLGSLSVTDHSQLRGVLSALAADEPEVRVALPSLR
                     VDSAAVKLGHLTSPTSQSVTLAPEAGSQRMRDVINKNVCEEDIFGAAREAFALGHTTL
                     KLYFMMGLPTETDDDVEAIADLCLRLRRLGKEQLGSRGSRLRLNVSVTNFIPKAFTPF
                     QWAPMADRAVLEARQMLLRRRLGVKGLKLSLHDIDSSYVEAALARGGEECAAIVEAAW
                     RAGARFDSWTEEARPQAWASAFSLAGASAEELATRARPLGDVLPWDVIEGVVSKEFLL
                     GEWERAASGVLTPDCRAAGCQDCGVCVGTLQIDEPGVGAPAGGASGGVHSGEVAQRRG
                     ATPDGSLRRWLITFSATGRARYLAHLDTMELFRRAIRRAGGRLALSGGMRPKPLLSLA
                     MPRGVGVEGLAELAEFTLQGPPVPEFAERLQQALPDGFRVRGFEPYHHARSAAARVVA
                     ARYRVVATCAPVDDGEPRTALTAEVLREAVRLYTRSSGIIVERRRPNGIRQIDVRTYV
                     ERIMAVVEEDTVVLGFVAAVTPAGTVRPEEVAAALSRLVDMPLVVSRIERLSLELDGT
                     TQQAV"
     gene            15644..17311
                     /locus_tag="VFD74_07890"
     CDS             15644..17311
                     /locus_tag="VFD74_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZK49504.1"
                     /translation="MKQLIVSSDSRESRVAVYEGNRLAELFVERRSRRSLVGDIYRAR
                     VENVLAGMDAAFVDIGLGKNGFLYVDEVQLPEESDARPRKITQLLRPGQEITVQVLKD
                     PMGTKGARLTTQLSLAGRYMVYLPGGSLCGVSRRLPDAERVRLRKLCHELKPDDAGVI
                     IRTAAEGVSDKAIARDLKFLAKLWQTVERRLESTGAPAIVYSEAELPLRLVRDMFTED
                     FSQILVDDEDLMKRMVGFLQATAPELSDRVELYKGRAPLFESHGVEAEVQAALKRRVD
                     LPSGGYLVIDKAEALTVIDVNTGRYVGKKFLEDTILKTNLEACAEVVRQLRIRDVGGI
                     IVIDFIDLSTRESREQVLALLEAELAKDRTKTYVVELSPLGLVEMTRQNITDGLRGIM
                     TDTCPTCRGEGVVLGDESLAISVERRLRRLAEAAAPEAFLVEVHPRVAGVLLRDDGAR
                     LRDLEKQTGRLFVIEGVGHLALEEFKISAEGSREVIMRASLPVVAGQVLEVAVEEENV
                     YDRRHGIARVDGYTVCVLGGAGVVGRRAKIVVEEATRYCAYARLADA"
     gene            17466..17765
                     /gene="rplU"
                     /locus_tag="VFD74_07895"
     CDS             17466..17765
                     /gene="rplU"
                     /locus_tag="VFD74_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZK49505.1"
                     /translation="MFAIVDIGGKQYRAEVGRELVVDRMTAEEGATVELDAVLISGDD
                     GAQFDEVKPAAVTATVVEHLRGPKLIIFKFKPKRGFKRKNGFRSALTRLSIDKIG"
     gene            17780..18034
                     /gene="rpmA"
                     /locus_tag="VFD74_07900"
     CDS             17780..18034
                     /gene="rpmA"
                     /locus_tag="VFD74_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZK49506.1"
                     /translation="MAHKKGGGSSRNGRDSNAKRLGVKAFGGQIVTAGSIIVRQRGTR
                     FRPGVNVGLGTDYTLFAKIAGRVVFARGKVGRTVSVVPAE"
     gene            18087..19505
                     /gene="obgE"
                     /locus_tag="VFD74_07905"
     CDS             18087..19505
                     /gene="obgE"
                     /locus_tag="VFD74_07905"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZK49507.1"
                     /translation="MFRDQAKIHVAAGRGGNGCTSFRREKHVPRGGPDGGDGGLGGDV
                     WLVADPQLRDLSPFARKVHYKAQAGRHGEGARRHGAAGSDREISVPLGTQAHRDGLLL
                     ADLTGRGQRVRVAAGGSGGRGNSRFVSSTRQAPHFSEFGDDGEESWIELSLRLMSDVG
                     LAGLPNAGKSSLLRRVSNATPKVADYPFTTIEPMLGVVQALGVEEPFTVTDVPGLLEG
                     AGDGVGMGTEFLAHLERCHMLLHIVDVAGYFGDDPRENFEIILRELVSGGTGLAVKPQ
                     VVVLNKIDLLSSVDLAVLTQYFVERVSTLRDEGHPAFSWTLSADPPEPQRLVWSVSTA
                     TGEGLPPLLTFVGSAVAELRVALEARGEAGVSPLGSEGSGVLTPGFGTDGLGAGAGHV
                     TFRPGADGPERFSVRREEDRFVIEGAWLGRLVRRYDLENDQAVRYLGERLERLGVSEA
                     LRAEGACPGDQVDIEGRSFEFQ"
     gene            19523..>20116
                     /gene="proB"
                     /locus_tag="VFD74_07910"
     CDS             19523..>20116
                     /gene="proB"
                     /locus_tag="VFD74_07910"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZK49508.1"
                     /translation="MADAHVMTVVVKIGSSTLTDERGKFRLGPMAGRVAEICDLHSKG
                     HQMVLVSSGAVSSGMGPLRMKSRPTEVVDLQAAAAVGQGRLYHTYSQLFGHEEVTTAQ
                     ILLTAMDIAARSQYLNARNTLRRLLEWGCVPIINENDTTSTDELCFGDNDTLAAQVAL
                     LVGADLLVLLTDTDGLFTANPTVDKGAAFISEVGDFSE"
BASE COUNT         3257 a         6194 c         7083 g         3582 t
ORIGIN      
        1 gacagggggg agggcaccac tctgctggaa tcgggcatca cccttccagt ggtggagatc
       61 gggctgctgg aagaaccggg cgcggatgtg gtggggccca cggatcacga gatactctcg
      121 tccctcgagg tgcgcatgca cctacccgag ggcggagtgc ccaaggaggg accgtcggcg
      181 gggctcgctg tggcggtgtc gctcttgtct gcgctcaccc tcatccccct gctgcctcgg
      241 gtggcactct cgggtgagat aaccctgacc ggacaggtcc tcgccgtagg cggactgaaa
      301 gagaagctgc tcgccgcgct gagggaaggg gtccggaagg tgatccttcc cgccgacgtg
      361 cggccgcaga tccaggaggt gcccgcggag ttgacccgag ggctcgagat cgtctacgta
      421 tcggagttca aggatgcact cgcgcatgtc ctgtgcgtgg agtgcgccgc ttccgccgac
      481 ggcgagagca cgccgccccg tcggcctagg aacgggtgag aaggagtcta cgtggctgag
      541 gtgacgatgc ccgcgcgcta ctctccgcat gaggtggagg agcgcctgat gagggagtgg
      601 ctcgatcgcg gagctttccg cgccgcgtcc gagccctcgg gaccgacgta ttccatcgtg
      661 atacctccgc cgaacgtcac cggttccctg catatgggtc acgccttgaa cggcaccatc
      721 caggacgtcc ttacgcgcta tcaccgtctg cgcggcgagt cggcgctctg gctcgtcggc
      781 accgatcacg ccggcatcgc tactcagaac aaggtggagg gccgcttggc cgctgagggc
      841 ctgcgcaagg aagacatcgg ccgtgaggcg ttcgaccgtc gggtctggga gtggcgggag
      901 cagtacgggt cgacgatcat caaccagttg aagcgtctcg gatgttcgtg cgactacgat
      961 cgcgaacgct tcaccatgga cgatgcctat cacgccgcgg ttctcaaggt gttcgtcgat
     1021 ctgtacgaga agggcgacat ctaccgcgac gtctacctgg tcaactggtg cgtgcgctgc
     1081 gggtccgcga tctcggatct cgaggtggag cacgtggagc gcgccgacag gctctactac
     1141 gtgcgctacc cggtggtcgg cgccgacgac ttcctgacgg tagccaccac tcggcccgag
     1201 acgatgttcg gggataccgc cgtggccgtg aaccctgacg atccacgcta cgggcggtat
     1261 gtgggcatga ccgctattgt gccgctggtc ggtcgcgagg tgcccatcat cgccgacgag
     1321 cacgtcgatc cggcgttcgg gacgggtgcg ttgaagatca cgccgggcca cgatcccaac
     1381 gacgcagaga tcgcccgccg tcatggtctg cccggcatct cggccatcgg cttcgacggc
     1441 ctgctcaacg aggaggcagg ggagttcgcc ggtcgcgacg ccgaggaggc ccgcgcggcc
     1501 atcgtgtcgc gcctcaccga ccatgggctg ttcgaacggg cggaggagta cacacactcc
     1561 gtggggacgt gctaccgctg cggcaccgtg atccagccgc tgctctcctt gcagtggttc
     1621 atggacatga agcggctcgc cgctccggcg atcgagtgcg tcgaggacgg gcgcgtcggc
     1681 ttcgtgcccg atcggtgggc cgacgtgtat ctggagtgga tgcgcggtat ccgcccctgg
     1741 tgcctcagcc gccaactgtg gtggggccac cgactcccgg tctggtactg cgacgactgc
     1801 agcaaggtgg tggtggccgt ggaggctccc acctcctgcc ccgactgcgg cggcacgttg
     1861 aggcgcgacg aggacgtgct cgacacctgg ttcagctcag ccatgtggcc gttcgccacg
     1921 ctgggatggc ccgagagcac gcccgagctc aagtcctact accccacctc cgccttgtcg
     1981 acagcgcgcg acatcctcta tctgtgggtg gcgcgaatga tcatgatggg gctggagtac
     2041 ttggacgacg tgcccttccg ctcggtcatc gtgcatccga ccatcctggc cgccgacggc
     2101 cgacgcatga gcaagagtct cggcacgggt atcgacccgc tcgaactgat cgacgaatat
     2161 ggcgccgacg cgacgcggtt cggcctcctc tacatgagct ccgtccagga tgtgcgcttc
     2221 agtgccgagc gcatcgaaat gggccgcaac ttcgccaaca agctgtggaa cgcttcgcgc
     2281 ctggtcctgc agggcgcgca cccggaggcg gtgccggcca tcgagctcgc gactccagcc
     2341 gaccgctgga tcttcagtcg gctcggggtc gtgtccgtcg agcttgcaca gttgtatgag
     2401 tcgttcgatt tcgccgaagc ggccagactc ggctacaggt tcgtgtggaa cgaggtgtgc
     2461 gactggtatc tggagatggc caagtcgcgc ctctacgggg atagtgaggc cgagcgactg
     2521 caggtgagcg gtaatctcct ggctcttctc gagaggatca ccggccttct tcacccgatg
     2581 atgccgttcg tgaccgccga ggtctatcgg aacctgcctc gcgcctcccc cggcgaaggg
     2641 caccagggtg cggccggcgc cggtgcagtc ggtgccgatg aagacgcggt cgacctgctg
     2701 tcaagccggt tccctgaggg ggatccggcc tgggccgatg ccgacgccga gagcgggatg
     2761 gagtcgttca tagcggtggt ggccggcctc cgctccgctc gggaggagct cggggtgcct
     2821 cgtggtgccg tgggtcgagt atggatcgtg acctcggatc cggggcgcgc ctcggctctg
     2881 accggggagc cgggggccct ccggcagctg gccggttgcg agatcgccgg tgtcgtgtgc
     2941 gacgagggcg atgtcccggc cggtcgcttc acttcggtgg acgctccagg cctgaaggtg
     3001 ctgctgccgc tcgagggtct cgtggatgtc gagaaggagc gcgcgaggct gatggggcga
     3061 gcagagaagg cgaaggcgga tgtggcgcgg gcgagcggca aactggccaa cgagcgcttc
     3121 gtggtcaacg cccccgcgga ggtcgttgcg caggaacagg agcggctggc ggccggccgg
     3181 gcgcagctag cggatatcgc ccggcagtat cgagaacgac tcggagagga actgccgtcg
     3241 tgagtggaac ggtttcagtg gaccaggcgc gggagtacgt gcatggtctc gagatgttcg
     3301 ggatgcgtct tgggctcgat cgtgtgcggg cgctggcgga gcgcctgggc gatccgcaga
     3361 aggcgtaccg caccgtgcac gtcgtgggca ccaatgggaa gtcttcgacc gcgcggttca
     3421 tctcggcgat ccttcagcag cacggtcttc gtgtgggcac ctacgtgagc ccccatctcg
     3481 tcagtctggc tgagcgtcag ctcgtcgacg gcgtggcctc caccgacgag gagttctatt
     3541 cgctggtggc acgcatcatg cccgtcgcgg ccgaggtcga ggctgagctg ccgaaagggg
     3601 aagtgctcac ccagttcgag gttctgacgg ccgcggcgta cctctacttc aaggagtccg
     3661 gctgcgacgt ggccgtcgtg gaggcgggac ttgggggtcg ctgggatgcg acgagcatca
     3721 tcgagtccga tgtgcaggtg atcaccacga tcgcgttgga gcacacggag tatctgggga
     3781 acacggcctt ggccatactc gaggagaagg cggccgtcat ccccgaaggc ggggcggtcg
     3841 tggccgggtt cctggacggc gacgtgagcg ctcggctcag gaccatctgc gcggagcggg
     3901 gcgcctctct gtgctcgctg ggcgaggaga tctccctgct caccgacatc ggccaggcta
     3961 cattcgacgt gttcggagcg cgcgatctct actcgaacct ccgcctgagc gtcctgggca
     4021 actaccagcg cgccaatgcc gctcttgcga tcgcggcggc ggaacagttc atgggtgcgg
     4081 cgctcgacgg ggccaagaca cgaaagggcc tgctgcacac ctcggtcccc ggccggctgg
     4141 aggtgatcag cgaccaaccg ttctgcgtgc tggacggtgc ccacaatccc tcgggcatgg
     4201 cggagatgct gcgctcactc gacggtcttc tagcccgccg acgccttctc gccgtggtct
     4261 cgatactcaa ggacaagggc gcgctcgaga tgctggccga cctcgcgccg cgctgcgaca
     4321 tcgtcttcgt gacgcagaac agcaatcccc gctcgtatcc ggcggaggag ctggccgcga
     4381 tattggaggg gctggacgac gctcctgagg tcttcgtcga cgcggaccct gagtctgcag
     4441 tgcggagcgc cctcaagctc gcgacgtcga accaggtggt gatcgtcacc ggatcgctct
     4501 acctggtatc cgatctgaaa cggtcgatgc agggaggctg atagggcgtg ggggcgaaga
     4561 gaagagacgg gcgcgacgtc ttcctgttca cggtcttcgc gctgctgttg atcgtcgtga
     4621 tatccttttt ggtcggatac tttgtgggca cccgccttct ctaggagagc gccttggaca
     4681 acgcattcca gaccatccag aacatcctta gctggccggg ttggacgctg gtgtggtatc
     4741 tgatctggtt ctcggtagtg gtgctgtggc tggcgctcgt cttctggacg ttgaaggacg
     4801 cgcggaagcg tatcgacgac ggactgatca ccgccgtggc agtggccacc tcgctggtgt
     4861 tcccgttcat cggcaccctg gtctactcca tccttcgtcc gcccgaatat ctggccgacg
     4921 tgcgtgaacg cgagctcgag atacacgcca tggagcaaga gctaatggag tcgcgggtgt
     4981 gtccgaactg ccgggagcct gtgcgcgaag gctacatcgt ttgcccgaag tgtcgcaaag
     5041 gtctgaggat cctctgcgtc acctgtgagc ggcctctcga gaagtcgtgg aaggtgtgcc
     5101 cctactgcgg cgccgacgcc gggccggtgg ccaaggtggc gccggtggct gaagtcgcca
     5161 acgggtgacg acggccgcgg cccgctcttg cgggccgcct agtcccgccg catcaccgat
     5221 gaactcgttt ctcgcacccg ggcgctggac gcgccccgat tggaggatgt cttgagcgtc
     5281 gaacgtacac ttgtgctggt gaaacccaac ggcgtcacgc gcggcctcgt gggcgaggtg
     5341 atcgggcgcc tcgagtgccg tggattcgtc ctggcggggc tgaagctcgt gcaggtcacc
     5401 aaggagctcg ccgaggagca ctatgaagag cacagcgaga agccgttctt cgaaggactg
     5461 gtgagcctct tgacctccgc gcccgtcgtc gccatggctg tcgagggcgt ctcggcggtg
     5521 caggtggtgc gcgacatgat gggtgcgacc gaccccacca aagcggcccc cggcaccatc
     5581 aggggcgact tcgcgctcga gatcggcgag aacgtggtgc acgggtcaga ctcggtggag
     5641 agcgctcgtc gcgagatcgc gctctacttc acgcccgaag aactcctgtc gtagtcgcgc
     5701 agaggagcgg cgcctccggc gccgtcctcc gcgggtgaac gccatgccgc tccccaccta
     5761 tccggaagac ttccgtctgc tcctggcgtc gagttctcct cgacggcggg acctcttgat
     5821 cgcagccggg ataccgttcg agacggtctc gactgacgcg gtggaggagg acatcggcac
     5881 cgatccgcgg cgcctggccg aacagaacgc gttgcgcaag gcgcggggcg cgcgtcttcc
     5941 cgcggggctg cacaccaagg tattcgtgct cggggccgac acagtcgtgg tggtgggcgg
     6001 tcgcatactc ggcaagcccg gggatgcggg cgaggccggg gatatgctcc ggcgtttgtc
     6061 ggccgtcgag caccacgtcg tcaccggggt cgcgctgatc gagctgggtg tggacggcga
     6121 ggtggcgcgt gaactggtgg gcgcctctgt aaccgcagtc cgtttccggt ccctttcggt
     6181 cggcgagatc gacgcctacc ttctgtcaga ggagtggcgg ggcaaggccg gagcgtacgc
     6241 gatccagggc ttggcttcgt tgttcgcgga ggggatcgaa ggggatcact cgaacatagt
     6301 ggggctacca ctcagcctgc tgggcgagct cttgcgtgag gcgggattcg accctgtgcg
     6361 tcgggtgtgg cgacggtgag cctcggggcc ttgcacccgt gctcgagcgg gcgatggtgt
     6421 tctcatcgca tccaggtata atccgtgccc tgggagaggg cctgccgggc cccgattaat
     6481 cgaagaggag acatccgata ggtttcttca gtctcataac cgggtttggc ggtcgcgaca
     6541 tggcggtcga cttgggcacg gcgaacacgt tggtctacgt gcgcggtcgc gggatcgtcc
     6601 tctcggagcc ttccgtggtg gcgatcgact cccgcaccgg cgaagtgcat gcagtgggcg
     6661 ccgaggccaa gcgcatgttg ggacgcacgc cggggaccat cacggcgatc cgacccctga
     6721 aggatggggt gatcgcggac ttcgacgtca cggagcagat gctccgtcac ttcatacaga
     6781 aggtgcacca gaaccgctgg gcacaccctc gcgtcgtcgt gtgtgtgccc tccggggtca
     6841 cgggggtgga gcggagggcc gtcgaagagg cgaccatcac cgccggcgcc cgcgccgtgt
     6901 atctcatcga ggaacccatg gcggccgcca taggcgccgg gctgccggtc agcgaaccca
     6961 ccggcagcat gatcgtggac atcggcggag gcaccagcga ggtcgcgatc atctctcttg
     7021 gcggcatcgt gaccgctcaa tccgtgcgga taggcggcga cgaactcgat gaagcgatca
     7081 tcgcctacgt caagaaagag tacaagttga tgatcggcgg ccagaccgcc gaggaactga
     7141 agctggagat cggttccgcg taccccatcg gcgaggacga gcacgccgag atccgtgggc
     7201 gcgacctcgt gaccggtctc cccaagacgc tggtcatctc gtccgaagag gtgcgcgacg
     7261 ccatcgcaga gcccgtgacg gcgatcgtcg acgccattaa agcgacgctc gacaagacgc
     7321 cgccggaact cgcctcggac gtcatggacc gcgggatcat gctcgccggc ggcggctcgc
     7381 tgctcaaagg actcgacgag cgcatccggc aggagacgca gatgccggtg cacctggcgg
     7441 agtctccgct cacctgcgtg gctgtgggct ccggtcgctc tctcgaagag ttcgaggctc
     7501 tacacaggag ctctcagaga acgagaaggc gccgctacta gcctagcgga gggcttccga
     7561 tgcaacgaag tgccgcactc agacggcgga tcgtgttcgc cgtccttatc gttatctccc
     7621 tcaccttgct gaccgcatac ttccgtgagc ctgcggccgg tctctttcac gtcgtccaga
     7681 acgacgcctc agacaccctg tcccccgtgc agtcacttgc cgcccgggcc gtgcagccct
     7741 tccgcgacgg atatcggtgg gcgggcgcgg tcctaggcgc gaacgccagg aacgagcagc
     7801 tcaacgcaca gctcgagtcg cttcgcggcg agctggtcct actcaacgag gcgagccagg
     7861 agaacgcgcg cctgaaggag ctgctcgact tcagggacgc cggcatattc ccggcgggat
     7921 cgacgttcga ggtggcccgc gtgatcggga agtcgcccac gacgtgggag gcgtgggtgc
     7981 agattgacaa gggctccgat gacgggatcg ccctaaacca gcccgtggtc ggagtgacgc
     8041 tgcccgcgga cagatccctc tccggcaagg ggctcgtggg caagatcacc gccgtgggca
     8101 agcgcagcgc ccgcgtgcag ctcatggtcg accaggcgtt caacgtcgcg gcacttgtcc
     8161 agggcactcg cgcccagggg atcgtcaccg gatcgctctc gggcagactg gtcatggact
     8221 tcgtcgagcg agaccaaccc gtagaggaga agctgatcgt gatcagctcg ggcttcggcc
     8281 agatcttccc gaaggggatt cccgtgggca tcgtgcagag cgtgggcgag gtcgacgtca
     8341 acatctacaa gcagatcgag gtgcggccct tcgtcgattt cgacacattg gaggaggtca
     8401 tgatcatcac caccccgacg gcgcaggtgg aagggtatga ggagatgagc acgctggttc
     8461 cccacgaagc cgagggaacc aagtgaggtg acggtatggt ccgggcacgc ttgatcgatg
     8521 tggggaggct ggcgctgctc ctcgtgctgg cggtcatcct gcagacgctc gctgcatccc
     8581 agctctcctt cctcggggtg accgccgacg tgttcgtgat cgtcgtggtg ctggtggcgg
     8641 tcggttccgg ctcgttcacc ggactcgtct tcggtttcgt ggcgggcctc acggcagatg
     8701 tcgtattcct cgacccgatc ggcatgcgca cgttcatgta cctcttgatc ggctactggg
     8761 ccggaaggta tgtggaggag ttcggattga gcagcgcatg ggtggtggtg cttctggccg
     8821 ggaccgtttc cctcggcgcg cagaccgtgt acgggatcct ccagatgatc accggcaccg
     8881 aggggtcgct cctcacgatg gtcgtgacgc aggtgctccc ggcagcgatg ttcgacggac
     8941 tgctctcggc tccgctctac ctcgggctca ccagggtcag gctgctacct caacagcggg
     9001 ccgccgggcc catgttcggg tagggcggca tgtacctgga accggtacgc aggcggcgcc
     9061 tgccggggga ggatcgctcc gcggggatgc ggctggcgat cctggcgacg gctgtcctac
     9121 tcctcttcgc tgtgctggtg ttccgcctgt ggttcctcca gatcctctcc ggcgaccagt
     9181 tcgccgccca agccgacaac caacgtctgc gcactgtgct gatacaggca ccccgagggg
     9241 tcatctacga taggaacggc agtctcctgg tgaccaatcg ggccggcctc agcgtgggta
     9301 tcctacccat ggaccttcgt tcgcctgaga cggtgatacc gcacctggcg gccgtgctgg
     9361 gcataccggc ggaagacatc agggcgaaac ttgacacggc cggacaggac gagtatcggg
     9421 tcgtcacgat caggcaggac gtgcccgaga acccggtggt gagctatcta ctcgagcaca
     9481 gcctggagtt ccccggggtg agggtggaga agtcgtacct gcgcgagtac ccgaacaaga
     9541 tcctcgcgac gcacatcttg gggtacgtgg gggaggcctc ctacgaagac ctgcagaagg
     9601 agcagttcca gaccctgaag ggtggcgcgg tcatcgggaa agacggggtc gaagcggtgc
     9661 atgactcgat gctgcggggc gtcgtcggca cccggacggt cgaggtggac gcggcgggtg
     9721 tgcccaagcg ggtgatccag gagaaccagc cgacccccgg cagcaacttg gttctcacca
     9781 tcgacagcgg gctgcaggcg gtcgcccgcg cggcggtcgt ggatggcatc gcgcgcgcac
     9841 acgccgacgg attcgtgaac tcatatgcag gtgtgatcgt ggctcttgac ccgcgcagcg
     9901 gcgagatcct tgcgatgacc tcctacccgg actacgaccc ctcgctgtgg gtgggcggca
     9961 tgagcacaga gacataccag aacctgatcg acccggccca gcacaaccca ctcttcgaca
    10021 gggccacgaa ggggctgtat ccggccgcct ccacgttcaa gcctttcgtg gccgcaacgg
    10081 ccctgcgcag cgatctgatc gactggacac agatcttctc cgacccgggg acgttcacgc
    10141 gcgaccagca ggtgtggcgt gactggctgc tacaggggca cggcgacgtg aacctagtgc
    10201 aggcgctggc cgaatcgtgc gacgtgtact tctacaacct gggctcgaga ttctacgacc
    10261 tgaaaggtcc cgtgctgcag gatggattga gggagttcgg cttcgggcag cgcaccggca
    10321 tagacctgcc gggcgagtat cccggccagg tgcccgacag tgagtggaag aagaagaacg
    10381 ggaagaccga gatcgaccgc atctggaagc cgggcgacga catcaacctg tcgatcggac
    10441 aaggtgacct tctggtcacg ccgcttcagt tggcgattgc cctggcggca atcgccaacg
    10501 gcggcgatat cctcgtgcct cgggtggctc gtcagataac cgacgcatcc gggaacgtga
    10561 cgaagcagtt cgagaccgaa gtgaagtcgc acgtggatat tggcgccgaa gacctggatg
    10621 ccatacggcg ggggctcaag atggttacga gcgaccccac gggcaccgcg tacggcgcct
    10681 tccgcgggtt tcccatcgcg gtggccggca agacggggac cgcgcagaag aagccgggcg
    10741 acgactacgc ctggttcatg ggatacgctc ccgcagacgc ccccgagatc ctggtcgttg
    10801 cgctgatcga gcagggtgga cacgggagtt cgatcgcggc tccagcggtg cgcaaggtcc
    10861 tggaagccta cttccgcacg gaatcctccg gccccgcgca ggtggaggtg accgagtgag
    10921 agtgctgcac ggaggcccat tgcgctcgac cagagggttc agcatgagtc ggtacctcgg
    10981 cagcctggac tgggtgcttc tggcagccac tgccggtctg gtgtgcttcg gtctcgccat
    11041 gatctactcg gctactcacg cggaccttac ggcgagcagc cccacccagt acgtctgggc
    11101 tcagagcttg gggctgatga tcggtgtgat cgcgctcgtg gtgctcagcc tcatcgactt
    11161 cacctggttc gcgcgtttgg gcaggtacct gtactggatg aacatcgggc tactcgcgct
    11221 gaccttggtg atcgggtcgg agcgcatggg tgcacggcgt tggatcagcc tgccgttctt
    11281 cgacgtgcag acgtctgaga taggcaaagt gttgctggtt ctcgctttgg cggcctttct
    11341 tgcggaaggg gtggagctgc ggggcagcct gcgcttcgtc ctgaaggccg tgatttatgt
    11401 cgcctttccg gcgaccttga tcttcctgca gcctgacctc ggcaccgcac tcgtcttcgt
    11461 cgccatcttc gtgtgcatgg tgctcgtgtg gagtattcgc tggtcgcacc tcgcgctcct
    11521 ggccggagcc gcggtcgcag cggccacgct ggtattgagg gtgctccccc gcacgttcgg
    11581 gatcgaactg ctgaggccct accaactcca gaggcttaca gtgttcgtca atccggggca
    11641 ggacacgtcg gatgccgcct atcaactctc acagagcaag atcgcggtgg cgagcggtac
    11701 gttcaccggc aaggggttca tgcagggcac ccagacccac ttgaacttcc ttccggcaca
    11761 ccacaccgac ttcattttct ccgtgatagc cgaggagttg ggtttcgtgg gttcgctcgt
    11821 gttgctgctg ctctttgctg tcgttctctg gcgcgcgttc cggatcgcga gcctttcgaa
    11881 gacgctccac ggcacgctga tcgccacggg tgcgatcggc gtgttggtgt tccaggtgtt
    11941 cgtgaacgtg gggatgacgc tcggcataat gcccatcacc ggactcccgc tgccgttcat
    12001 cagcttcggc agcaacagcc tgatggtatt cttgatcctc gtgggtttgc tccagagcgt
    12061 gcacgtgcac tcgcgaacgg cgctctacgg agggcgtctc aaaggagacc cgtatggcca
    12121 aatggccgct tgacccgaac aagatatggg gcgccttcaa ggagatcgcc gctgcgaccg
    12181 cacaggacgc cggcatcgtc ctggccgggg acccgtcact cacggccaaa gcccaggagg
    12241 cgttgtcgcc cgacggatgg cacgcgcggc tctggcaggg ggctgctgag ggtcttgcgg
    12301 ggctcgccct cggcccgggt gacgtgttgc tcgtgctcgt gcagcccaac gaggaggatg
    12361 tctgggcggc ggccgtgcgc gccgccgcgc tgcctctggg ggcggtcgtc gtcgtggacg
    12421 acggacccgc cgccactcag cgcaccacat ggtatgacaa gcacagggcc cgagtctcct
    12481 tctcgccgac gcccgagggc tggacggcgg tgtggaccgc cgtcgtggag gtgggaggtg
    12541 accatgtcgt ggctctcggg cgccggttcc ccatcctgcg ggggtgggca tcgcgcaaag
    12601 tcatcaagcg cacggcccgg cagaacggtc tcgtcggtgc cgcgttcatc gtccccggca
    12661 cggacatgcc gatcatgacc ctgaaccaga tcaagatggt gctgtcgatc gccgccatcc
    12721 acggcgagga gatcaccacg gagcgtgcga tcgaactcgt gggggtcgtc ggctcgggat
    12781 tcggcatgcg tgccgtggcg cgccaagcgc tcgacctggt gcccgggcct ggttggctgt
    12841 tcaagggggc agtgggctac acaggaacgc gtgccctcgg cgaggcagcc cttcgctact
    12901 tcgacctggg tgcgcctgct actccgggcc gtctctcttc tctggctcgg cggatcaaga
    12961 ggtgacgcgc gtcgcggtgt cgccggactt gggcggcatc catcggcgct tcgagggcat
    13021 cctgggtcgg gtccagaatc ctgcgcgcct ggtgggtggc gagatcggtg cgggccccgg
    13081 attcagcggt cggacgggcg agctgcgcgt cgccctcgcc ttccccgacg catacgaggt
    13141 cgggatatcc aaccaggcgc ttcagatcct ctatcacgtg gcaggcacgg ttaccggcgt
    13201 cggcgtggag agggcatacc ttccgtgggt ggacgcgata gccgagatgc ggcgcgaggg
    13261 cgtgccgctc cttacccttg agacgtggac tcccgtgaag gccgtgcatt tgctgggaat
    13321 caccctgcag cacgagctga actacaccaa tgtgcttgaa ctcctggatc tggccgggat
    13381 cccggtgcgg acggcggacc gaggagaaga cgccccactc gtggtggccg gcggtcccgc
    13441 gaccgcggac ttcctgccga tggccccgtt cctggacgcc gtcgtggtgg gtgacggtga
    13501 agaggtgttc gccgagttgc tggcgagtct ggtcgaggcg ggtgcggccg gcgaagggcg
    13561 cgcggagcgc gtgcggcgat tggcgggcat cagaggcgtg ttcgtgcccg ggtcgagccg
    13621 catcgtgcga cgggtggcgc tcgacaggct cgaagatgcc ccgtacccgg agcactgcct
    13681 ggtgccgctc acagcagggg tccacgatcg cgcctgggtg gaagtggcac gcgggtgttc
    13741 gcggggctgc cgattctgcc aggccggcat gtggtatcga cccgtgcgag agaggagccg
    13801 cgaccggatc gtggagttgg ccggctcgca actgcgcgcg agcggccacc aggaggtgag
    13861 tctcggatcg ctctccgtga ccgaccactc gcagctgcgg ggagtgctga gcgccttggc
    13921 cgccgacgag cccgaggtga gagtggcctt gccatctttg cgcgtggaca gcgctgccgt
    13981 gaagctgggc cacctgacct cgccgacgag ccagtcggtc actctggctc ctgaggcggg
    14041 cagccagcgc atgcgcgacg tgatcaacaa gaacgtgtgc gaagaggaca tcttcggcgc
    14101 ggctcgggaa gccttcgccc tggggcacac cacactgaag ctctacttca tgatggggct
    14161 ccccaccgag accgacgacg acgtggaggc gatcgccgat ctctgccttc gtctgcggcg
    14221 gctggggaag gagcagctcg ggtctcgcgg gtcgaggttg agactgaacg tcagcgtcac
    14281 caacttcatc ccgaaggcgt tcacgccgtt ccagtgggcg cccatggccg accgtgccgt
    14341 gctcgaggct cggcagatgc tgcttcgcag gaggctgggt gtcaaggggc tgaagctctc
    14401 gctccacgac atcgattcga gttacgtgga agcggccctc gcccgcgggg gagaagagtg
    14461 cgcggcgatc gtagaggccg cttggagagc cggtgcccgc ttcgactcct ggaccgagga
    14521 ggcacggccg caggcttggg cctctgcgtt ctcgcttgcc ggagcgtcgg cggaggagct
    14581 cgccacccgg gccaggccgt tgggcgacgt gctcccgtgg gacgttatcg agggagtggt
    14641 ttcgaaggag ttcctcctcg gtgagtggga gcgagccgcg tcgggggtgc tcacccccga
    14701 ctgccgcgcc gccggctgcc aggattgcgg ggtgtgcgtg ggcaccctcc agatcgacga
    14761 gcctggggtg ggcgctcctg cgggtggtgc gtccgggggt gtccattccg gtgaggtcgc
    14821 ccagcgacga ggcgcgacgc ccgacggatc cctgcgccgc tggctcatca ccttctcagc
    14881 tacgggcaga gcgcgttacc tcgcccacct ggataccatg gagctgttcc ggcgggccat
    14941 ccgtcgggcc ggtggccgcc ttgctctgtc gggagggatg aggcccaagc cgctcctgtc
    15001 gctggccatg ccgcggggtg tcggggtaga ggggttggcg gaacttgcgg agttcacgct
    15061 ccagggccct ccggtgccgg agttcgcgga gcgtctgcaa caagctctcc ccgacggctt
    15121 cagagtccgg ggcttcgagc cctaccacca cgcgcgcagc gccgccgcac gcgtggtcgc
    15181 cgcccgctat cgcgtggtcg cgacctgtgc gccggtcgac gacggggaac ctcggaccgc
    15241 gttgacggcg gaagtgttgc gcgaggcggt cagactgtat actcgctcgt cgggaatcat
    15301 cgtcgaacgt cgtcgaccca acgggatccg acagatcgac gtgcgcacct acgtggaacg
    15361 catcatggcg gtggtggagg aagataccgt tgtcctcggc ttcgtggccg cggtgacgcc
    15421 cgccggcacg gtgcgacctg aagaggtcgc agccgcgctc tcacgtcttg ttgatatgcc
    15481 gctggtcgtg tcgcggatcg agcggctcag tttggaactt gatggaacga cgcagcaggc
    15541 cgtctgaccg gtccgtggta cgacagcccc gcgcttgcgg ggtggtcgcg agcgggctgg
    15601 gacggactgc agacgcccgt cgttcgaaag gttgtggtgt agcgtgaagc agttgatcgt
    15661 ctcgtcggat tcgcgcgaga gtcgcgtggc ggtgtacgag ggcaaccggt tggcggagct
    15721 ctttgtggag cgtcggtcgc gtcggtctct cgtcggcgac atctaccggg cccgggtgga
    15781 gaacgtgctg gccggcatgg atgccgcttt cgtcgatatc ggcctgggca agaacgggtt
    15841 cctctacgtg gacgaggtgc aactccccga ggagagcgac gcccgtcccc ggaagataac
    15901 ccagctctta cggccgggcc aagagatcac cgtgcaagtg ctcaaggacc ccatgggcac
    15961 caagggggcc cgcctcacca cgcagctcag tctggccggt cgctacatgg tgtatctccc
    16021 cggaggcagc ctgtgcggcg tctcgaggcg tctgccggat gctgagaggg tgcgtctgcg
    16081 caagctgtgc cacgagctca agcccgacga cgccggcgtc attatccgca cggctgccga
    16141 gggggtgagc gacaaggcga tcgcgcgcga cctcaagttc ctcgcaaagt tgtggcagac
    16201 cgtcgagcgt cgcctggagt cgacgggggc tcccgcgatc gtctactccg aggccgaact
    16261 cccgctgcgg ttggtgcgcg acatgttcac ggaggacttc tcccagatcc tggtcgatga
    16321 cgaagacctc atgaaacgga tggtcgggtt cctgcaggcg acggcccccg aactgtccga
    16381 ccgcgtggag ctgtacaagg gtcgcgcgcc gctcttcgag agccatggcg tggaagccga
    16441 ggtgcaggcg gcgctcaaac ggagggtgga tctgccctcc ggcggctacc tcgtgatcga
    16501 caaggcagag gccctcacgg tcatcgacgt caacaccggg cgctacgtgg gcaagaagtt
    16561 cctcgaggac accatcctca agaccaacct cgaagcgtgt gccgaggtgg tacgccagct
    16621 gcgtatccgt gacgtgggcg gcatcatcgt catcgacttc atcgatcttt ccacgcgcga
    16681 aagccgggag caggtgctcg ccctgctgga agccgagctg gcgaaggacc gcaccaagac
    16741 gtacgtggtg gagttgtctc cgctcggatt ggtggagatg acccggcaga acatcaccga
    16801 tggcctgcgc ggcatcatga cggacacctg ccctacgtgc cgcggcgaag gcgtggtcct
    16861 gggcgacgag agtctcgcca tctctgtcga gcgtcgcctg cgccgcctgg cggaagcggc
    16921 ggctcccgag gccttcctgg tcgaggtaca cccgagggtg gccggggtgc tcctgcgaga
    16981 cgacggtgcg cgactccgcg atctcgaaaa gcagacgggc agactgttcg tcatcgaagg
    17041 agtgggacac ctcgccctgg aggagttcaa gatcagcgcg gagggctcac gcgaggtcat
    17101 catgcgggcg tctcttcctg tggtcgccgg acaggtcctg gaggtggctg tggaagaaga
    17161 gaacgtatac gaccgtcgtc acggcatcgc gcgggtggac ggctatacgg tgtgtgtgct
    17221 cggaggggcc ggcgtcgtgg gtcggagggc caagatcgtg gtcgaagagg ccacgcggta
    17281 ttgcgcctac gctagactgg cggacgcgta gctggactct cgtgggcggc ctgccccgga
    17341 atgggagggc cgcggttccg tcgtagtggt cggagggcgg aggccttggt atactactgc
    17401 gtttgcggcc cccgggtggt cttggctggc ggtgaaggtg gatcgatcga gtcttaggag
    17461 aacggatgtt tgccatcgtt gatataggcg ggaagcagta tcgggccgag gtcggccggg
    17521 agcttgtcgt cgatcgcatg accgccgaag aaggcgctac agtggaactc gacgccgtcc
    17581 tgatctccgg cgacgacggg gcgcagttcg acgaggtgaa gcccgcggcg gtgacggcca
    17641 ccgtggtcga gcatctgcgc ggccccaagc tcataatctt caaattcaag cccaagcggg
    17701 gcttcaagcg caagaacgga ttccgcagcg cactcacgcg cctgtcgata gacaagatcg
    17761 gttagggagg ggtcgaggta tggctcataa gaaaggcggc ggtagttcga ggaacggtcg
    17821 tgactcgaac gccaagaggt tgggagtcaa ggcgttcggc gggcagatcg tgaccgccgg
    17881 gtcgatcatc gtgcggcagc ggggcacccg tttccgcccc ggtgtcaacg taggtctggg
    17941 caccgactac acgctgttcg ccaagatcgc cggtcgggtg gtcttcgcca gaggcaaagt
    18001 cgggcgcacc gtcagcgtcg ttcccgcaga gtagtctccg cctcttcgag gggtcggcct
    18061 agccgtcccc gagggccggt ccgcacatgt tcagagatca agccaagatc cacgtggcgg
    18121 ccggccgagg cgggaacggg tgcacttcgt tccggcgcga gaaacacgtg ccgaggggcg
    18181 ggcccgacgg aggggatggc ggtctcggag gcgacgtgtg gctggtcgcc gacccgcaac
    18241 tgcgagacct gagtcctttc gcccgaaagg tgcactacaa ggcgcaggcc ggtcggcatg
    18301 gcgaaggcgc ccgcaggcat ggcgccgccg gctctgatcg cgagatatcc gtcccgctcg
    18361 gcactcaggc gcaccgcgac ggcctactct tggccgactt gaccggccgc ggccagcggg
    18421 tccgggtggc ggcgggcggg agcggcggcc ggggcaactc gcgattcgtc agctccaccc
    18481 ggcaggcccc ccacttctct gagttcggcg atgatgggga ggagtcttgg atcgagctct
    18541 ccctgcggct gatgtcggat gtgggactgg cggggttgcc gaacgccggg aagtcgtctc
    18601 tcctgcgccg tgtctccaac gccaccccga aagtggccga ctacccgttc accaccatcg
    18661 aaccgatgtt gggagtggtg caggccttgg gagtcgaaga gcccttcacc gtgaccgacg
    18721 tgcccggact tcttgaaggg gccggcgacg gcgtgggcat gggaacggag ttcctcgcgc
    18781 acctggagcg gtgtcacatg ctccttcaca tcgtggacgt ggccggctac ttcggcgacg
    18841 acccgcgcga gaacttcgag atcatcctcc gggagttggt gagcggaggc accggccttg
    18901 ccgtcaagcc gcaggtcgtc gtgctgaaca agatcgatct gctgtcgagt gtggatctgg
    18961 cggtgctcac ccagtacttc gtcgagcgtg tctccacact ccgcgacgag ggacatccgg
    19021 cattctcgtg gacgctctcc gcggacccgc cggagccgca gagacttgtg tggtctgtgt
    19081 ccacggctac cggggagggg ttgccgcctc tgctgacgtt cgtgggctct gccgtggcgg
    19141 agctgagggt cgcgctcgag gcgaggggcg aggccggagt ctcaccgctc ggaagcgagg
    19201 ggtccggtgt cctgactccc ggtttcggga ccgacggtct cggggctggt gcgggccacg
    19261 tcaccttccg gcccggagcc gacggtccag aacgattctc tgtgcggcgt gaagaggatc
    19321 gtttcgtcat cgagggcgcg tggttgggcc gtctcgtgcg gcgctacgac ctggagaacg
    19381 atcaggccgt ccgctatctt ggggagcggt tggagcgtct cggggtcagt gaggctctgc
    19441 gcgcggaggg agcatgcccc ggagaccagg tcgacatcga gggtcggtcg ttcgagttcc
    19501 agtagcagaa gggggagtcg gcgtggcgga tgcgcatgtg atgaccgtcg tggtcaagat
    19561 cgggtcgagc acgctcaccg atgagcgcgg gaagttccgc ctgggcccga tggcgggtcg
    19621 ggtggcggag atctgcgacc tgcattcgaa gggacaccag atggtgctcg tgtcatccgg
    19681 cgccgtgtct tcggggatgg ggccgctgcg catgaagagc cgtcctacag aggtggtgga
    19741 cctccaagcc gccgccgcgg tggggcaggg gcgtctctac cacacgtaca gtcagctctt
    19801 cggccacgag gaagtgacca ccgcacagat ccttctcacc gccatggaca tcgccgcgcg
    19861 ctcacagtac ttgaacgcgc gcaacaccct gaggcgcctt ctggagtggg gctgtgtccc
    19921 gatcatcaac gagaacgaca ccacaagcac cgacgagctg tgtttcggcg acaacgacac
    19981 gctcgccgcg caggtcgcgt tgctggtggg tgcggatctg ctcgtgctcc tcaccgatac
    20041 ggacggcctc ttcaccgcca accccaccgt cgacaagggt gcggccttca tctccgaggt
    20101 gggcgacttc tcggag
//
LOCUS       DASRMJ010000286         4895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_28760, whole genome shotgun sequence.
ACCESSION   DASRMJ010000286 DASRMJ010000000
VERSION     DASRMJ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28760"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFD74_07915"
     CDS             <1..395
                     /locus_tag="VFD74_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49509.1"
                     /translation="HGRRAEILEHHRLLLGKRRKTQPRGSRTFGSTFRNPPGDYAGRL
                     LESSGLKGVRRGGAQVSTVHANFISNLGDATTADVLALMVMMRGTVAERQGVFLEPEV
                     RLIGADFPWGGRDGGPHRRVSPSATQPG"
     gene            395..1168
                     /locus_tag="VFD74_07920"
     CDS             395..1168
                     /locus_tag="VFD74_07920"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZK49510.1"
                     /translation="MDERFRDRRRQVGRSRGKRRLTVVLVVALSVGAALGFYWLRSSG
                     VFAVVKVSLPVTQNVSEAQIRAVVAPVSGVNLLRVSTQTLEASLREIPYVRSAHVYRG
                     FPDSLVIDIEEYVPVARVRASDGTEWLVADDGTILAEAGAEAGDLLLLSAQADVRPQV
                     GVVTAPQVVGALRLVSWLEDGVTWPVSVHPVKEVSVLRSGELAIVLQGGGEVRLGDPE
                     QLDEKLTVALEIIDRYLKDGKELEYVDVHVPSRVVAKAR"
     gene            1316..2404
                     /gene="ftsZ"
                     /locus_tag="VFD74_07925"
     CDS             1316..2404
                     /gene="ftsZ"
                     /locus_tag="VFD74_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZK49511.1"
                     /translation="MIDTGSSYLAVIKVVGIGGGGTNAVNRMVDAGLKGVDFIAVNTD
                     AQALLMCDADVKIHIGGKITRGLGAGANPEVGRQAAEESRDELREALKGADMVFVTAG
                     KGGGTGTGAAPVIAELARELGALTVGVVTKPFTFEGRKRASQAEQGIEALSRKVDTLI
                     IIPNDRLLQVVEKRTSIIEAFKVADDVLRQGVQGITDLITVPGLINLDFADVRTIMRN
                     AGSALMGIGVASGENRGVEAARAAISSPLLESSIEGATGILLNITGGKDMGLFEVNEA
                     AEIISGAADAEANVIFGAVIDEGLGDQMRVTVIATGFAPRAIMQDRAEKSEDGTEATG
                     GFALDLEKPRFEMDDDILDIPAFLRDKD"
     gene            2412..3278
                     /locus_tag="VFD74_07930"
     CDS             2412..3278
                     /locus_tag="VFD74_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HZK49512.1"
                     /translation="MAVTAAGRRVLAAIEERIDPVARDRFEVVAPVGLPLIVAKTPER
                     FAVAFTTRLVGVEAGSPGHRFDLSMDPGSSPRPRHGRHREHLSTALAELAGTAAPSLV
                     SPTQVHGVRVVGAAEYARDGCASACDGLTVQPGLDSGLAALLVFADCVPVVLLGEIDG
                     AVVHGGWRGLLAGVVQQGAASMTAPPGLAIIGPSIGPCCYTVGEKVALDFERRYGDGV
                     VLPGRRLDLWEAATRAAGEVGVPRSRVVNPRLCTVCNRDLFFSYRADGPGTGRHGTVL
                     WCAAGDVPVGSA"
     gene            3318..4013
                     /locus_tag="VFD74_07935"
     CDS             3318..4013
                     /locus_tag="VFD74_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZK49513.1"
                     /translation="MPVDIEHLRKNVEQATARIEAAHARGGHGQRVRLLVATKYVTAE
                     DMSVLHEAGVRLVGENRAEELELKWSVWGEAFEFHFIGHLQRRKVRGVVPRVKMVHSV
                     DSLRLAEEMHQRAEAPIDALLEVNMSGEESKYGILPGDVEAFLEQAQALESVRFRGFM
                     TMAPLVRDPEEVRLVFRRLRELRDRISPLFTPRYLLDELSMGMSNDYEVAVEEGATIV
                     RLGSALFACAQGG"
     gene            4017..4574
                     /gene="sepF"
                     /locus_tag="VFD74_07940"
     CDS             4017..4574
                     /gene="sepF"
                     /locus_tag="VFD74_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZK49514.1"
                     /translation="MALGDVWHKTLVYFGIAEEDERYDEETLAPHEELEQSYRERPNV
                     KRVERESRATGRRRSASGADFDEIYANDDAFRNQRTAVIRPVPPPVETPSVKVHLVVP
                     KNFNDAQLIADKFKAAIPVILNLQGCETELSKRLIDFASGLTYALDGGMQRVADKVFL
                     LTPKNVEVSAEERQRLMEKGFFNQF"
     gene            4648..>4895
                     /locus_tag="VFD74_07945"
     CDS             4648..>4895
                     /locus_tag="VFD74_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZK49515.1"
                     /translation="MTEKAAIHTIGVIGTGSMGTALVRGLLAGTPARTVFQVFDREPE
                     QARALAALDGSRITHVSELAELVTGSDPVIISVKPQDM"
BASE COUNT          852 a         1443 c         1753 g          847 t
ORIGIN      
        1 cccacggccg ccgagcggag atcttggagc accaccggct cctactgggc aagcggcgca
       61 agacgcagcc gcgggggtcc cgcaccttcg ggagcacctt ccgcaacccg ccgggggact
      121 acgcgggcag gctcctcgaa agctccggct tgaagggcgt ccgtcgcggc ggggcccagg
      181 tctcaacggt gcatgcgaac ttcatctcga atctcggtga tgctacgacc gccgacgtat
      241 tggcgctcat ggtcatgatg cgagggactg tcgccgaaag gcagggcgtg ttcctcgaac
      301 ccgaggttcg gttgatcggc gccgacttcc cttggggggg gagagacggg ggcccacacc
      361 gccgagtctc cccatccgcg acacagccgg gctgatggac gagcgcttca gagaccgccg
      421 ccggcaggtg gggcggagcc gaggcaagcg tcggctgacg gtggtcctcg ttgtggcgct
      481 ctcggtcggg gccgctctcg gcttctactg gcttcgctcg tccggcgtgt tcgccgtggt
      541 caaagtgtcg ctgccggtca ctcagaacgt cagcgaggcg cagatccgtg cggttgtggc
      601 gccggtctcc ggggtcaatc tgctccgggt gtctacccag actctggagg caagtctgcg
      661 agagatcccc tatgtgcgat cggcgcacgt ctaccgtggt tttcccgata gcctggtgat
      721 cgacatcgag gagtacgtcc cggtggctcg agtgcgggca tccgacggga cggagtggtt
      781 ggtggctgac gacggcacca tactcgcgga ggcgggtgct gaggccggcg acctgctcct
      841 cctctcggct caggcggacg tgaggcccca ggtgggagtg gtgacagcgc ctcaggtggt
      901 cggtgccctg cggcttgtct catggctcga agatggggtc acgtggcccg tctccgtgca
      961 cccggtgaaa gaggtgtcgg tgctgcgttc gggcgaactc gccatcgtgt tgcagggggg
     1021 cggggaagtc cgtctaggag accccgaaca gctggatgag aagttgacgg tggcgctcga
     1081 gatcatcgac cgttacctga aggatggcaa ggaacttgag tatgttgatg ttcacgtgcc
     1141 ttctcgagtc gtagctaagg ccaggtgagc gtggtatcgt gcatggaggc aggaatcccc
     1201 gccaagctac agtatgagtg gagattcgca tgaacatggc tggagtacag gtcgagccaa
     1261 ccgggcgacc gctccagatc tgtacgtccg tgactggtat ggaggtatgt gagtgatgat
     1321 cgataccggt tccagctacc ttgcagtcat caaggtcgtg ggtatcggcg gcggtggcac
     1381 gaatgccgtc aatcgcatgg tcgacgccgg attgaagggt gtcgatttca tcgcggtgaa
     1441 caccgatgcg caggcgttgc tgatgtgtga cgccgacgtg aagatccaca tcgggggcaa
     1501 gatcacccgg ggactaggag caggggccaa cccggaggta ggccggcagg ccgcggagga
     1561 gagccgggat gaactgcgcg aggccctgaa gggcgcggac atggtgttcg tcacggccgg
     1621 caagggtggc ggcacgggca caggagccgc tccggtcatc gccgagcttg cgcgggaact
     1681 cggggctctg acggtgggcg tggtgacgaa gccgttcacc ttcgaggggc gcaagcgggc
     1741 gtcccaggcg gagcagggca tcgaagctct gagccgcaag gtcgacacgc tgatcatcat
     1801 cccgaacgac cgccttctgc aggtggtcga gaagcgcacc tcgatcatcg aagcgttcaa
     1861 ggtcgccgat gacgtcctcc gccagggcgt gcaaggcatc accgacctga tcaccgtccc
     1921 agggttgatc aaccttgact tcgcggacgt gcgcaccatc atgcgcaacg ccggttcggc
     1981 gctcatgggc atcggggtcg cgagcggtga gaaccgtggc gtggaagcag cgcgcgccgc
     2041 catctcctcc ccgctcctcg aatcctcaat cgagggcgcc accggcatcc tgctcaacat
     2101 cacgggcggc aaggacatgg ggttgttcga ggtgaacgag gcggctgaga tcatttcggg
     2161 agccgccgac gccgaggcca acgtcatctt cggggcggtc atcgatgaag gactcggcga
     2221 ccagatgcgg gtgacggtca tcgccaccgg cttcgcgcca cgagccatca tgcaggaccg
     2281 ggcagagaag tccgaggacg gcacggaagc gaccggcggg ttcgcgctcg acctcgagaa
     2341 gccgcgtttc gagatggacg acgacatcct cgacataccc gcgttccttc gagacaaaga
     2401 ctagctccgg ggtggcggtg acggctgctg ggaggagagt cctggcggct atcgaggaga
     2461 ggatcgatcc ggtcgcacgg gatcggttcg aggtggtagc gcccgtcgga ctcccgctga
     2521 tcgtcgcgaa gacccctgag cgtttcgcgg tggcgttcac gacgagactc gtcggtgtag
     2581 aagcgggatc gccggggcac cggttcgacc tgagcatgga ccctggatct tccccgcggc
     2641 cgcgacacgg acggcatcgc gagcatctga gcaccgccct cgccgaactg gccgggactg
     2701 cagcgccgtc gctggtgtca cccacccaag tccacggcgt gcgcgtggtg ggggcggccg
     2761 agtacgctcg cgacgggtgc gcatccgcat gcgacggcct caccgtgcaa cccggactcg
     2821 atagcggtct cgcggcgctg ctcgtcttcg ccgactgcgt accggtggtg ctcctcggtg
     2881 agatcgacgg agcggtggtg cacggggggt ggaggggact cctggctggg gtggtgcagc
     2941 agggcgcggc atccatgacc gctccaccgg gcctggcgat catcggtcca agcatcgggc
     3001 cgtgctgcta cacggtgggc gagaaggtcg ccctcgactt cgagcgtcgc tacggtgacg
     3061 gggtggtctt gccgggccgc cggctcgacc tctgggaggc ggctacacgc gcggcggggg
     3121 aagtcggcgt accgcggtcg cgggtggtga acccccggct gtgcacggta tgcaaccggg
     3181 atcttttctt ctcctatcgc gccgatgggc ccggaaccgg ccggcacggc accgtgttgt
     3241 ggtgcgccgc aggcgatgtc ccggtaggct ccgcgtgatg tcacgcctcg aaacaagctg
     3301 acgggcagag aggggaaatg ccggtcgata tcgaacatct caggaagaac gtcgaacagg
     3361 cgaccgcgcg gatcgaagcc gcgcacgccc ggggaggcca cgggcaacgg gtccgcttgt
     3421 tggttgccac caagtacgtg accgccgagg acatgtccgt tctgcatgag gcgggggtcc
     3481 gcttggtcgg tgagaacagg gcggaagagc tcgagttgaa gtggagtgtt tggggggagg
     3541 ccttcgagtt ccacttcata gggcacttgc agcgccgcaa ggtgcggggt gtcgtcccgc
     3601 gggtgaagat ggtgcactcc gtggattcgc tcaggttggc ggaggagatg caccagcgcg
     3661 cggaagcgcc gatcgacgct ctgctggagg tcaacatgag tggagaagag agcaagtatg
     3721 gtatccttcc gggcgatgtg gaggcttttc ttgagcaggc tcaggccttg gagtctgttc
     3781 ggttcagggg cttcatgact atggccccgc tcgtgcggga tcccgaggag gtccgtctgg
     3841 tgttccggcg gctgcgggaa ctccgggacc gaatctctcc tctcttcacc ccccggtact
     3901 tgctcgatga gctctcgatg ggaatgagta acgattatga ggtggcggtc gaggaagggg
     3961 cgacgatagt gcggctgggc agcgcgctct tcgcctgtgc gcaaggagga tgagtaatgg
     4021 ctttgggtga tgtctggcac aagacgcttg tctacttcgg gatcgccgag gaagacgagc
     4081 gctacgatga agagaccctc gcgcctcatg aggagctcga acaatcgtat cgggagaggc
     4141 cgaacgtgaa gcgggtggag cgcgagagtc gcgccaccgg gcgccgcaga tccgcgagcg
     4201 gagccgactt cgacgagatc tacgcgaacg acgacgcgtt ccgcaatcag cgcacggccg
     4261 tcatccggcc ggtcccccca ccggtggaga caccctcagt caaggtccac ttggtggtgc
     4321 ccaagaactt caacgacgcc caactcatag ccgacaagtt caaggcggcc atcccggtca
     4381 tcctcaacct tcagggctgc gagacggagt tgtcgaagcg tcttatcgac ttcgcgagcg
     4441 gcctcaccta cgccctcgac ggggggatgc agcgggtggc cgacaaggtc ttcttgttga
     4501 ctcccaagaa cgtggaggta tcggctgagg agcggcagcg tttgatggag aagggcttct
     4561 tcaaccagtt ctagccccgg atcccgcccc gaggccggcc gctgaggtcg taggcgggtc
     4621 cgtcaggggg aggagatcgt tcctagcgtg acagagaagg ctgctatcca caccatcggg
     4681 gtcatcggta cggggagcat gggcaccgcc ctggtccgcg ggctccttgc gggcacaccc
     4741 gcccgcaccg tgttccaagt gttcgacagg gaacctgagc aggcgcgggc gctggcggcg
     4801 ctcgacggat ccaggatcac ccacgtgtcc gagctcgccg agctcgtcac gggctcggat
     4861 ccggtgatca tctcggtgaa gccccaggat atgga
//
LOCUS       DASRMJ010000287         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_168805, whole genome shotgun sequence.
ACCESSION   DASRMJ010000287 DASRMJ010000000
VERSION     DASRMJ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168805"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="dprA"
                     /locus_tag="VFD74_07950"
     CDS             <1..1041
                     /gene="dprA"
                     /locus_tag="VFD74_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZK49516.1"
                     /translation="EHSCREIWQADRATLLRWSGGRAWVDRFIRGRSSVSSEATLAAC
                     RAARLRFIAHGAADYPESLRRLHQPPAGLYVAGDGIALEALAARPRVTIVGTRRPTSY
                     GMTVAARLAAAFAARGVCVLSGLALGIDGRAHRATLDAGGITAAVVGCGADVVYPGRH
                     RELREKIVRRGVVLSEYAPGCSATPWSFPERNRVLAALGAAVVVVEAPERSGALITAR
                     RAAELGKDVFAVPAPILTRESVGCNRLIYDGAIPCLDPDEVVEDFLRATRMERGGREP
                     LVASADALDGRGDADGAERLVLLALAARSLTIDEVVQAVGLTMREASVALAMLEVAGK
                     VVRRGPGKYAVV"
     gene            1106..2086
                     /locus_tag="VFD74_07955"
     CDS             1106..2086
                     /locus_tag="VFD74_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZK49517.1"
                     /translation="MTEPPEKTAIEGQCFLYLRSLVAAGYSPRSVKAVRSDLAQFCAY
                     CASLAVLDATQVTAPHVRAFVSGLVEGSLSPRKRPYARTSVARKLSTVRVFLGFCERE
                     GFREGNPGHGIHAPRLPKRLPSVLTPTQIGEFLDGIQGDDPLTLRDRALFELIYSCGL
                     RAQEVLDLAVRDIDAGRREARVRGKGRKVRVVPVGDDALLAVDRYVETARPVLLAASR
                     SDARAVDDLRRERERREAPLFVSRRGRALSPSDVRRRLERHLDRSSVPEGTSPHTLRH
                     SFATHLLEGGADLRSIQELLGHASLATTQVYTHVSAAHLRKAYRTAHPRA"
     gene            2094..2891
                     /gene="whiG"
                     /locus_tag="VFD74_07960"
     CDS             2094..2891
                     /gene="whiG"
                     /locus_tag="VFD74_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HZK49518.1"
                     /translation="MGLKKVGSEVEELWGRYKLHGDQKARDELILAYSPLVKYVAGRM
                     GTGLPAHVEESDLISYGLLGLIGALERFDPGRNIKFETYAISRIKGSIIDELRSLDWV
                     PRSVRSWARQVEKAVTKLENELGRAPSDEETSGELELTVSEFHDILHQISSASIVALD
                     EFWTLAGSDGDKVSLMDTIEDSDSPDPSRAYGIREVKEILAGAIRRLPEREKIVIGLY
                     YYENLTLKEIGEVLGVTESRISQLHTKAILRLKGRIKEDLEPGALSA"
     gene            3116..>3564
                     /gene="rpsB"
                     /locus_tag="VFD74_07965"
     CDS             3116..>3564
                     /gene="rpsB"
                     /locus_tag="VFD74_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZK49519.1"
                     /translation="MPAASMKQLLESGVHFGHQTRRWNPKMKPFIFADRGGIHIIDLQ
                     KTTKLLDRAYEFTRNISSRGGSVLFVGTKKQCQDAIRDEATRVGMPFVSHRWLGGLLT
                     NFTTIHGRINRLHELRHHQEDGTLALLPTKERMNLERELEKLETNLGG"
BASE COUNT          608 a         1113 c         1212 g          631 t
ORIGIN      
        1 gaacacagct gccgcgagat atggcaggcc gaccgagcga cgctgctgcg ctggtcgggc
       61 ggccgggctt gggtcgatcg attcatccgc ggcagatcgt cggtgtcatc ggaggcgacg
      121 ctcgccgcct gccgtgccgc ccggttgcgt ttcatagccc acggggctgc cgactacccc
      181 gagagcctga ggcgtcttca tcaaccgccc gcgggactgt acgtcgcagg cgacggcatc
      241 gcgctggagg cgctcgcggc gcgccctcgt gtgaccatcg tcgggaccag gcgcccgacc
      301 agctacggca tgacggtcgc ggcgaggctg gcggctgcct tcgcggcgcg cggagtgtgt
      361 gtgttgagtg ggttggccct gggtatcgac ggccgggccc atcgggcgac tctcgatgcg
      421 ggcggaatca cggcggccgt ggtcggctgt ggggccgatg tcgtgtaccc ggggaggcac
      481 cgcgaactgc gcgagaagat agtccgccgc ggggtggttc tgagcgagta tgctccgggc
      541 tgctccgcca cgccctggtc gttccctgag cgcaaccgcg tgctcgcggc tctgggagcc
      601 gccgtggtgg tcgtcgaggc gcccgagcgc agtggcgctc tgataacggc tcgacgggcg
      661 gcggagttgg gcaaggacgt cttcgctgtg cccgcgccta tcctgacccg ggagagcgta
      721 ggctgtaacc ggctcatcta cgacggggcg atcccgtgtc tcgatccgga cgaggtcgta
      781 gaggatttcc ttcgggccac gagaatggag aggggaggcc gcgagccgct cgttgcgagt
      841 gcggacgccc tcgacggacg gggggatgcg gacggcgcag aacggctggt tctgctcgcg
      901 ttagctgctc gctctctgac catcgacgaa gtcgtgcaag cggtgggact gaccatgcgg
      961 gaggcgtcgg tggccctcgc tatgctcgaa gtggcgggga aggtcgtgcg acgcggaccg
     1021 gggaagtacg cggtcgtttg atgtgcgcgc cggtcactca ccgacgcgag gcgccgagtg
     1081 agggagcgtg tcgagtatcg ccacagtgac cgagccacct gagaagaccg cgatcgaagg
     1141 gcagtgtttc ctgtacctgc gcagtctcgt cgcggcggga tacagccctc gaagcgtcaa
     1201 ggccgtccgc tccgacctcg cccagttctg cgcctactgt gcgtcactgg ccgtccttga
     1261 cgccacacag gtcacggcgc cgcacgtgag ggccttcgtg tcgggtctgg tcgaggggag
     1321 tctctcccct cggaaacgac cgtacgctcg gacctccgtg gcccgcaagc tctcgacggt
     1381 ccgggtcttc ctcggcttct gtgagcgcga agggttccgc gagggaaatc ccggacacgg
     1441 catacacgcc ccccgcctcc ccaagaggtt gccgagcgtg ctcacgccca cgcagatcgg
     1501 ggagttcctg gacggcatcc aaggcgatga cccgctcaca ttgcgagacc gggcgctttt
     1561 cgagttgata tactcgtgcg gtctgagggc tcaggaagtg ctggacctgg ccgttcgtga
     1621 catcgacgca ggaagacgag aggcgcgggt gagaggaaaa ggacgcaagg tccgtgtcgt
     1681 cccggtgggg gatgacgctc tgctggccgt agaccgctat gtggagaccg ctaggccggt
     1741 gttgctcgcg gcctcgcgga gcgatgcccg tgctgtcgat gaccttcgac gcgagcgcga
     1801 acgccgggag gcgcctctgt tcgtgagtcg ccgcgggcgc gcgctcagcc catcagatgt
     1861 ccgccgtcgt ctcgagcggc atcttgaccg ttcctccgtt cccgagggca cttctcccca
     1921 tacattgcgg cactccttcg ccacccacct gctcgagggc ggtgccgatc tacgctccat
     1981 ccaggaactc ctgggccacg cctcgctggc taccacccag gtctacacac acgtatccgc
     2041 cgctcacctg cgcaaggctt atcgcacagc gcacccgagg gcgtagggcg acgatgggac
     2101 tcaagaaggt cgggagtgaa gtcgaggagc tgtggggtcg ctataaactc catggggatc
     2161 agaaggctcg ggatgagttg atcctggcgt attcgccgct ggtgaagtac gttgccggtc
     2221 gcatgggcac agggttgccc gcgcacgtgg aggagtccga tctgatctcc tacggcctcc
     2281 tcggcctcat aggcgctctg gagcgcttcg acccggggcg caacatcaag ttcgagacgt
     2341 acgccatctc ccgcatcaag ggttcgatca tagatgagtt gcgctccctc gattgggttc
     2401 cgagatccgt ccgcagctgg gcccgccagg tggagaaggc ggttacgaag ctcgagaacg
     2461 aacttgggcg cgcgccctca gatgaagaga catctggcga actggaactc accgtcagcg
     2521 agttccacga catcctccat cagatcagca gcgcttcgat cgtagccctc gatgagttct
     2581 ggacgctcgc cgggtcggat ggcgacaagg tgagcctcat ggacacgatc gaggactccg
     2641 attctcccga tccttcccgg gcgtatggca tacgcgaggt caaggagatc ctggccgggg
     2701 cgatccggcg cctgcccgag cgggagaaga tcgtcatcgg tctctactac tacgagaacc
     2761 tgactctgaa ggagatcggt gaagtcctcg gggtgacgga atcgcgcatc tcccagttgc
     2821 acacgaaggc catcctccgc ttgaaagggc gcatcaagga ggatctggag ccgggcgccc
     2881 tctcggcctg agagacgggt cctgtggcgt gtcctccctg cctgagcggg cggccttcgc
     2941 aatcgcatgg aatcttggtg aggcaactgg gggcatcggc ctgggcgtgc ccgtgtgcta
     3001 cacttcttcg gttgtcaacc ccgaccccga agaaggccac cacggtggtg ccggccgacc
     3061 cggttccgtg gggtgtttcg ggtggaagca cacagaaccg agaaaggaag tgcccatgcc
     3121 tgctgcctca atgaagcagc tcctggagtc cggagtacat ttcggtcacc agacccgccg
     3181 gtggaacccc aagatgaagc ccttcatctt cgcggatcgt ggcggtatcc acatcatcga
     3241 cctgcagaag accacgaagc tgctcgaccg tgcctacgag ttcactcgca acatctcgag
     3301 tcggggcggt tccgtcttgt tcgtgggcac gaagaaacag tgtcaggacg ctattcgcga
     3361 cgaagccacc cgagtgggca tgccgttcgt ctcgcatcgt tggctgggtg ggttgctcac
     3421 caacttcacc acgatccacg ggcgcatcaa ccggctgcac gagctgcgac accaccagga
     3481 agacggcacg ttggctctcc tgcccacgaa ggagcgcatg aacctcgagc gcgaactcga
     3541 gaagctggag acgaatctcg gagg
//
LOCUS       DASRMJ010000288         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_425523, whole genome shotgun sequence.
ACCESSION   DASRMJ010000288 DASRMJ010000000
VERSION     DASRMJ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425523"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFD74_07970"
     CDS             complement(<1..633)
                     /locus_tag="VFD74_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49520.1"
                     /translation="MSLAAPVSAVGASLDLSLAISLDGPWIDASTVVEVRGPGSPMAG
                     GAEWPVVARIERREGDLGSGEFGLDITLQPEDLPEPGAYLLSVDLIGADGKRLTTRSW
                     CGRISNLPARVDLAVVLPVVSGIRRDPEGAFIDDLVQRAVVPEADQEGSLYGLFSAVE
                     QNRDWHLTLGVEPLFLAQLRDLADGYRERIGGGAVREVPAGEGAARDAEQA"
     gene            913..2367
                     /locus_tag="VFD74_07975"
     CDS             913..2367
                     /locus_tag="VFD74_07975"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HZK49521.1"
                     /translation="MGLDQSAGIFYKTRVRDVMTSAAITCHQETQVSEMALQMKSHNT
                     GSVVVVGDDDHPLGIVTERDLVYKVVATGAGNLTAGEIMSSPPVTVHPDDFVYQAVGL
                     MMRRRCRRLIVVHEAGGLAGFISMRDLMRLQAYDHRIIMERVAKAHTIDDLRQIRPEI
                     DEFVHRLFLADVDGPSLAEILTDFNDTVSQRIIFMAEMRLRAEDVRPPLSGFAWLTFG
                     SEGRREQVLRGDQDNGIVLEEAADVERARSYYRELARRVNEDMAAYGFALCEGGVMAR
                     EDKYFGTASEWRQRIAELVSGCGDGKKLRDLTIILDMRHLAGDEHLTSDLWQHTLRCM
                     HANPPAMRALAEDATSKGIPLNFLGRFRYEKDPEGRRGINVKKFGMLPLTAGVKAVAI
                     EHRVTGTATPERITALEHQGVLERNSAADLRFAHELFLRLKLQASVETVFHQQADTHF
                     FYPEDWTDLERSNLRRAFRAVDSLLSFLRVRYSL"
     gene            2393..>2819
                     /locus_tag="VFD74_07980"
     CDS             2393..>2819
                     /locus_tag="VFD74_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49522.1"
                     /translation="MELILSTGSIRGVEASSLFTIAQAAGFEGLEYVVTPAHTAAHVN
                     ELKAIAARTGLHIRNVHAPYMPVPGWGDQIQALKLTVELADHLGARSVTFHPPQRVIQ
                     DVDFARWIVATSDFQRDVGNSRVAVSMENMPKVRSWRGFR"
BASE COUNT          525 a          957 c          916 g          421 t
ORIGIN      
        1 ggcctgctcc gcgtcgcgag cggccccttc accggccggg acctctctga cggcgcctcc
       61 accgatgcgt tcgcggtatc cgtcggccag gtcgcggagt tgcgcaagga acagcggttc
      121 cacgcccaag gtgaggtgcc agtcgcgatt ctgctcgacg gcgctgaaca gaccgtagag
      181 gctgccctct tgatcggctt cgggcacgac cgccctctgc acgagatcat cgatgaacgc
      241 gccctcggga tccctccgaa tgcccgagac gaccggcagc accacggcga gatccactcg
      301 cgcgggcagg ttggagatac ggccgcacca ggatcgggtg gtcagacgct tgccgtcggc
      361 gccgatgagg tcgacggaga ggaggtacgc gcccggttcg ggcaggtcct ccggctgcag
      421 agtgatgtcg aggccgaact cgccggaccc gagatcgcct tcccggcgct cgatccgcgc
      481 caccaccggc cattcggcgc ccccggccat gggagagccc ggcccgcgca cctcgaccac
      541 ggtggatgcg tcgatccagg gtccgtccag ggagatggca aggctgaggt caagggaggc
      601 tccgacggcg ctgacgggag ccgcgagtga cactgcaagg gacgactccg cgaaggcggg
      661 cgaggcgagg aagaacgacc ccgcgcaggc gaggatcgcg gctgcgatcc aggcatgaag
      721 acgagacgag acccgtcttg ggacacgggc ggtgaccgac gaaagggagg cgaccctcat
      781 ggcacggaag tgtagcacag ggcatcaggg cctcgagccc gccgcgcgag cccactcctg
      841 gtagaatccg gtggtggtcc tcgcgcccgc tgcgagagaa cgtcaccaag gggggtccgt
      901 tcggtgagcg gcatgggtct cgaccagagc gctggaatct tctacaagac gcgcgtgcgc
      961 gacgtcatga cttccgcggc catcacctgc caccaagaga cacaggtgag cgagatggcc
     1021 ctccagatga agtcgcacaa cacggggtcc gtggtggtgg tcggcgacga cgaccacccg
     1081 ctcggcatcg tgacggaacg cgatctggtg tacaaggtcg tggcaaccgg agcgggcaac
     1141 ctcacggccg gcgagatcat gtcgagccca ccggtgacgg tgcaccccga tgacttcgtc
     1201 tatcaggccg tgggcctgat gatgcgccgc cgctgccgcc gcttgatcgt cgtgcacgag
     1261 gcgggcggcc tggccggctt catctcgatg cgcgacctga tgcgcctgca ggcctacgat
     1321 caccgcatca tcatggagcg ggtggccaag gcgcatacca tcgacgacct acgccagatc
     1381 cgacccgaga tcgacgagtt cgtccaccga ctgttcctgg ccgacgtcga cggcccctcg
     1441 ttggcggaga tactcacaga cttcaacgac accgtctccc aacgcatcat cttcatggcc
     1501 gagatgcgcc tacgggctga agacgtgcgc ccgccgctga gcggcttcgc atggctcacg
     1561 ttcggctccg aagggcgacg agaacaggtg ctgcggggcg accaggacaa cggcatcgtc
     1621 ctcgaggaag cggccgacgt cgaacgcgca cgaagctact accgcgaact ggcccgccgc
     1681 gtgaacgagg acatggcggc ctacggcttt gctctctgcg agggtggcgt catggctcgc
     1741 gaagacaagt acttcggcac tgcctcggag tggcggcagc gcatcgccga actggtgagc
     1801 ggttgcggtg acggcaagaa gctccgagac ctcacgatca tcctcgacat gcgtcatctg
     1861 gcgggcgacg agcatctcac gagcgacctc tggcagcaca ccctcaggtg catgcacgcc
     1921 aacccgcctg ccatgcgcgc gctcgccgaa gacgccacga gcaaagggat cccgctgaac
     1981 ttccttggac gcttccgata cgagaaagac ccggaaggcc gtcgcggcat caacgtgaag
     2041 aagttcggca tgctgccctt gacggcgggc gtgaaagcgg tcgccatcga gcaccgggtc
     2101 accggcaccg cgacccccga gcgcatcacc gctctggagc accagggtgt cctcgaacgc
     2161 aactccgcgg ccgacctccg cttcgcgcac gagctcttcc tgcggctcaa gctacaggcg
     2221 tccgtggaga ccgtcttcca ccagcaggcc gacacccact tcttctaccc ggaggactgg
     2281 acggacctcg aacgtagcaa tctgcggcgt gcattccggg cggtcgactc tctgctctcc
     2341 tttctccgag tccgctacag cctgtagagc gggacggggg aacgcacctg tcatggaact
     2401 catcctctcc acgggctcca tccgcggcgt ggaagcctcg agcctcttca ccatcgccca
     2461 ggctgccgga ttcgaaggcc tcgagtatgt ggtgactccc gcccacacgg ccgctcacgt
     2521 gaacgagctg aaagccatcg ccgcccggac cggactgcac attcggaacg tacacgcccc
     2581 atatatgccc gtgcccggat ggggggacca gatacaagcc ctcaagctca ccgtcgagct
     2641 cgcggaccac ttgggggcac ggagcgtcac cttccacccc ccgcagcgcg tcatccaaga
     2701 cgtggacttc gcccgctgga tcgtcgcgac gtcggacttc cagcgcgacg tcggcaacag
     2761 ccgcgtcgcg gtgagtatgg agaacatgcc gaaagtccgc tcgtggcgag gcttcagag
//
LOCUS       DASRMJ010000289         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_29238, whole genome shotgun sequence.
ACCESSION   DASRMJ010000289 DASRMJ010000000
VERSION     DASRMJ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29238"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..1685
                     /locus_tag="VFD74_07985"
     CDS             531..1685
                     /locus_tag="VFD74_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZK49523.1"
                     /translation="MIYLDNAATSCPKPPAVVEAVRRTLTEVCANPGRGAYDDALEAG
                     RILLRTRRSVARLCNIRDEARVVFTLNATHALNLALKGILGTGGNVVTSSLEHNSVVR
                     PLAALAARGVTVTRVGCTADGSTDPDDILAALRPDTRLVVLTSASNVLGTLLPVAEVG
                     PTLRERGVPLLVDAAQTAGSRPLDVEAMCVDLLALPGHKGLLGPQGVGVLYVGPGFRL
                     ETLMEGGAGSDSISVDPPEDLPDRYEAGTPNTPGIAGLGVGVDLVMKRGVEDVGKHED
                     RLTELLRAGFDDTEGITVYGPPAGVSRAPVVSVRFAGLPAAEAAFVFDRAFGIAVRAG
                     LHCAPEAHRIAGTLEEGLVRFSIGHANTQADIDAAIEAGREVAAKAGMRS"
     gene            1734..2747
                     /locus_tag="VFD74_07990"
     CDS             1734..2747
                     /locus_tag="VFD74_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZK49524.1"
                     /translation="MKPIDTGIPVRGESPNSTPHWEVSDPNEAAALATRIIDNVGTVI
                     RGKRGVARDCVVALLAGGHLIIEDFPGVGKTMLAKALALSIRCRFSRIQFTPDLLPSD
                     VTGVNIFSQKTGEFEFRPGPIFANVVLADEINRASPKTQSALLECMQERQATIDSCTY
                     PISAPFMVIATQNPIEYEGTFPLPEAQLDRFMLRISVGYPARDDESSMLEDQTSRDPI
                     ADLRPILDSGQLLGLIESTRRVRATKAIRDYVVALMDATRSAPDFYLGASPRAGIGLV
                     RAAKAGALLDGRDFVTPQDVKDLAVRVLSHRVILSPDGRLHGRTTASVIEKILDTVPV
                     PSG"
BASE COUNT          505 a         1011 c          891 g          468 t
ORIGIN      
        1 cggcgatatt cagtagtgcg atccgttcga tctcgacgcc gaatggatgt gacgacgaca
       61 cgtcgtcgaa catcttcccg actgcgaacg caccggtaac acttgtcagc agaaacaggg
      121 cggcaaccgc cagtactgct aatacggagt agcgtctagt catcatttcc tttcatggat
      181 ggttgatact tcaatcttca ttcgcgtcga ataggccggt cgaacgcatc acccccctct
      241 cgtcttgccc tacatgccaa caacacggca cggcaaagcc gatcaaggat tggattcccc
      301 cgactccgcc gagcggccgc ccctcgcacc gctagacaaa cccagcgcga taatacctat
      361 tgtttcatat tcaatgagcc aaaggcaaac cgtggcgcac gaagacttcg tcgccgacgt
      421 atggcccgcc cctgctagaa tgccggggaa gctggacttg ccaccacagt gccggtgccc
      481 gaagcgcgcc cccctcgttg cgccggaact ggacccacaa aggacactcg atgatctacc
      541 tcgacaacgc cgctacctcg tgtccgaaac cgcccgcagt ggtcgaggcc gtgcgccgca
      601 cgctcacgga ggtgtgtgcg aaccccggac gcggggccta cgacgacgcc ctggaagccg
      661 gccgcatcct gttgcgcact cgacgctccg tggcgcgctt gtgcaacatc cgcgacgagg
      721 cgcgcgtcgt gttcacgttg aatgccaccc atgctctgaa cctggcgctc aagggcatcc
      781 tgggcaccgg agggaacgta gtgacctcgt cgctggaaca caactccgtg gtgcgcccgc
      841 tggcagcgct ggccgcccga ggtgtcaccg tcacgagagt gggctgcacc gcagacgggt
      901 ccaccgaccc tgacgacatc ctggccgcgc tccggcccga cacacgcctt gtggtgttga
      961 cctcggcctc gaacgtcctg ggcacgttgc tgccggtggc ggaggtcggg ccgacgttgc
     1021 gggaacgcgg cgtccctctg ctcgtcgacg ccgcgcagac ggccggatcc cggccgctcg
     1081 acgtcgaagc catgtgtgtc gacctgctgg cccttccagg ccacaaaggg ctacttggcc
     1141 cgcagggtgt gggggtcctc tacgtgggcc cgggcttccg cctggaaacg ctgatggagg
     1201 gaggagccgg ctccgattcc atctcggtgg acccaccgga ggacctgccc gaccgctatg
     1261 aggccggcac ccccaacaca ccgggcatcg ccggtctcgg cgtgggcgtc gacctcgtga
     1321 tgaagcgcgg cgtcgaggac gtgggcaaac atgaggatcg tctcaccgag cttctccgag
     1381 ccggcttcga cgataccgag ggcatcacgg tctatgggcc tccggcgggc gtgtcccgcg
     1441 cccccgtggt ctcggtccgt ttcgccggcc tgccggcggc cgaggcggcg ttcgtgttcg
     1501 atcgcgcgtt cggcatcgcc gttcgggcgg gtctgcactg cgcaccggaa gcccaccgca
     1561 tcgccggcac gctcgaggaa ggactggtgc gcttcagcat cgggcacgcc aatacccaag
     1621 ccgatatcga cgccgccatc gaggccggcc gggaggtggc cgccaaggcc ggcatgcgca
     1681 gctagatcga acacccccac ccgccgacgc gggagacacc cagggaggag aacgtgaagc
     1741 ctatcgacac cggtatcccc gtccggggcg agagccccaa ctcgacaccc cactgggagg
     1801 tgtcggaccc gaacgaggcc gccgctctgg ccactcggat catcgacaac gtgggcaccg
     1861 tgatccgagg caagaggggc gtggcccgag actgcgtcgt tgccctcttg gccggcgggc
     1921 acttgatcat cgaggacttc ccaggggtcg gcaagaccat gctggcgaaa gccctggcgc
     1981 tctccatccg ctgccgcttc tcccgcatcc aattcacccc cgacctgctc cccagcgacg
     2041 tcaccggggt caacatcttc agccagaaga ccggcgagtt cgagttcaga cccgggccga
     2101 tattcgccaa cgtggtgctt gcggacgaga tcaaccgggc gtctcccaag actcaatccg
     2161 cgctgctgga gtgcatgcag gagcgtcaag ccaccatcga cagctgcacg tatcccatct
     2221 ctgcgccttt catggtcatc gcgacccaga acccgatcga gtacgagggc accttccctc
     2281 tgcccgaagc ccagctcgac cggttcatgc tgcgcatctc cgtcggctac ccggcgcggg
     2341 acgacgaatc gtcgatgctc gaagaccaga cctcgcgaga cccgatcgcc gacctccggc
     2401 ccatcctcga ctccggccaa ctcctcggtc tgatcgaatc tactcgtcga gttcgggcga
     2461 ccaaggcgat ccgcgactac gtggtggcgt tgatggacgc gaccagatcg gctcccgact
     2521 tctacctggg agcgagcccg cgggccggta tcgggctggt gcgggcggcg aaggcgggcg
     2581 cgctgctcga cggacgggac ttcgtgaccc cccaagacgt gaaagacctg gctgtgcggg
     2641 tgctctccca tcgcgtcatc ctgtcacccg acgggcgcct gcacggccgc accacggcct
     2701 ccgtgatcga gaagatactc gacaccgtgc ccgtaccctc gggctgacga ggggagccga
     2761 cgtgaccaga cgcggcttcg gcatcctgct gctcgccgcc ggcgcctacg ccgcggggcg
     2821 tctgctcggc acgcacgagc tctacctaat ggcagcggcc ttcgcagcat cggtg
//
LOCUS       DASRMJ010000290         6349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_535232, whole genome shotgun sequence.
ACCESSION   DASRMJ010000290 DASRMJ010000000
VERSION     DASRMJ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535232"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFD74_07995"
     CDS             <1..467
                     /locus_tag="VFD74_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49525.1"
                     /translation="VEITMGEQVAGTGGTVHIGSAAGTGSEGATGSAGGMGGTGSAGG
                     AGSTDDTGGIVTVRVGRVGESCVCESRVEQPGLDVVTRSVDIAPVAEDRLLAEELDQL
                     GPDRVFEESLLLAAALLRLPGGELVASAGPGPSELAEAPYGSPAASAGITGQ"
     gene            530..1138
                     /gene="pyrR"
                     /locus_tag="VFD74_08000"
     CDS             530..1138
                     /gene="pyrR"
                     /locus_tag="VFD74_08000"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZK49526.1"
                     /translation="MPTDDLSEAAGAVDGSLAPVGKVLLDRERLHRTLVRMAHEIAEH
                     DPDVGAMALVGIQRRGVEIAQRLASHLEEFYGARPATGAIDITFYRDDLDNRGPAFDQ
                     PVVHSTSLPFDVQDRRIVLVDDVLFTGRTIRAAVEALFDYGRPAAVQLAVLVDRGHRE
                     LPIRPDFVGKNVPTSRTERVNVSLEEIDGRDEVTLEHPPDTR"
     gene            1189..2109
                     /locus_tag="VFD74_08005"
     CDS             1189..2109
                     /locus_tag="VFD74_08005"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZK49527.1"
                     /translation="MHLISIDDLSVADIETLFRLSDGFLEVASRPIKKVPTLRGRTQI
                     NMFFEASTRTLTSFELAGKRLSADVVNIKASGSSVGKGESLKDTILTLESYRPDVMVI
                     RHDRVGACRMATRYTNACVVNAGDGSGEHPTQCLLDLYSLRREFGVLEGLKVAIVGDV
                     LRSRVARSNVFGFRKMGIDVRLVGPPTLMPPGIGEWGVPTYETLEALVDVDVVYLLRM
                     QLERAKGRVEPVPSLREYSRVWGLAPAKLRSGQRVMHPGPINRGVELTGDLADDDRSL
                     ILQQVESGLAMRMAILYHVLAPHGEGDAAT"
     gene            complement(2106..2507)
                     /locus_tag="VFD74_08010"
     CDS             complement(2106..2507)
                     /locus_tag="VFD74_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49528.1"
                     /translation="MGNTCYDCGAENAEVDAFCRACGASLARTADLWKPGIQAGSSEE
                     RVLWEKGDIRLTTDAVLIGMSTDSPDVVPLETVHEVVVEDRCVVLRVKDGDDKYCLLD
                     DPTELADLVRDHVFRSRLAQERKERGFTPSD"
     gene            2768..4084
                     /locus_tag="VFD74_08015"
     CDS             2768..4084
                     /locus_tag="VFD74_08015"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZK49529.1"
                     /translation="MFDPTRGLDERGSLVVRDGVIVALGAISEPPPGATVLSEVAGCH
                     VFPGFVDMHTHLRTPGQEYKEDLESAGRAAAAGGFVMVTGMANTDPVVDSGPLATWVL
                     DQAESRAAVAVAHVGAVSKGSRGGSLAELRELVDAGVVALSDDGRPLEDMDLLLAALR
                     YLRITRRPLLLHLEDLTLSTDAAMHEGAWSARLGLRGVSGAAEAGPMARDLEVLRVVM
                     AEESARDAGRAGAGGAPAPRLHFQHLSTEAAVRLLRAAKADGLPVTAEVTPHNLVFTD
                     ERVASFEQSCKVNPPLRSERDRAAVAAALAEGIIDCVATDHAPHASHEKEVPFEDAPS
                     GTIGLETAFAAIYGGLVASGGLTLERLIDALSAAPCRCLGIDPPRLEVGAPADLCAVD
                     LTEEWTVGVADLHSKSRNSAFLGERVQGRVRLTVVRGARRFERGRP"
     gene            4136..5326
                     /gene="carA"
                     /locus_tag="VFD74_08020"
     CDS             4136..5326
                     /gene="carA"
                     /locus_tag="VFD74_08020"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZK49530.1"
                     /translation="MYRSEIPGYVVLEDGTVFRGFGFAVEGRVLGEIVFNTGMTGYQE
                     VTTDPSYHGQMVTFAYPMIGNYGAADALLESADVHSRAIVVREVKNTNYNRTCPTAWV
                     DWLHGASVVGVGGVDTRALTRRIREHGAMNACVAAGAHLKVDELLEEVRAQPSLSGRD
                     LVREVTCSEPYVVGSANARHHVVAYDFGMKRSILRNLLEAGCRVTVVPAGWTAAQVLE
                     LEPDGVFLSNGPGDPQPVTYAVEAIRELLGRVPVFGICLGHQLIALALGLTTYKLKFG
                     HRGINHPVKNYLSDSIEITSQNHGFAVAPPPAVERAWAEGTDLSSIPAADMVVESDLG
                     RVQVTHLNLNDGTVEGLRLLDRPAFCVQYHPEASPGPHDSLYLFREFTESMRRSGRVQ
                     AGRL"
     gene            5430..>6349
                     /locus_tag="VFD74_08025"
     CDS             5430..>6349
                     /locus_tag="VFD74_08025"
                     /inference="COORDINATES: protein motif:HMM:NF010589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HZK49531.1"
                     /translation="MVAFQRSLTAIRALGPENGGEGEWERSRFLLDQARRFGFTDIVE
                     VDAEDERVPGGRRPNLILRVPGRSAHPAVWVMAHMDTVPPGETALWDSDPFVAEVCDG
                     RIVGRGVEDNQQGLVAGLFALRALLDEGVTPTTDVRLLLVADEETGNARGVDHVLETR
                     PALFDPSDLVIVPDAGAPDGAVLEIAEKSALWIRLAVRGKQVHASVPQAGVNAHRAAA
                     HLVVRLDRRLHQVFDAEDGLFEPPGSTFEPTKREANVQNVNTIPGEDVLYFDCRILPR
                     YGLAAVKAEVEEEAREIAAEFGVRVDVSYPT"
BASE COUNT         1020 a         2068 c         2197 g         1064 t
ORIGIN      
        1 cggtggagat caccatgggc gagcaagtcg ccgggaccgg cggcaccgtg cacatcggca
       61 gcgccgcggg gaccggtagt gagggtgcca ccggtagcgc gggcggcatg ggcgggaccg
      121 gtagcgcggg cggcgccggc agcaccgacg acaccggcgg gatcgtgacg gtgcgcgtgg
      181 gtcgggtggg agagtcgtgc gtgtgcgaat cacgggtgga gcagcccgga ctggacgtcg
      241 tcacccgttc cgtcgatatc gctcccgtgg cggaagaccg cctgctggcc gaggagttgg
      301 atcaactggg ccccgatcgg gtgttcgagg agtctttgct cttggcggcc gcgctcctgc
      361 ggctccccgg gggcgaactc gtcgcctccg cgggcccagg gccttcggag ctcgcggagg
      421 cgccctacgg gtcgccggcc gcctcggccg gcatcaccgg tcagtgagcc gcatccggtg
      481 ctagaatcct gacgcaaccc gatccggatc acccgaggga ggcacaggta tgccgactga
      541 cgatctgagc gaagctgcgg gcgcggtgga cggatcgctc gcccctgtcg gcaaggtcct
      601 gcttgatcga gaacgactcc atcgcacgct ggtgcgcatg gcccacgaga tcgcagaaca
      661 cgaccccgac gtcggcgcga tggcattggt gggcatccag cgccgcggcg tggagatcgc
      721 gcaacgtctc gcctcccacc tggaggagtt ctacggggcg cgtcccgcga cgggcgcgat
      781 cgacatcacg ttctaccgcg acgacctgga caaccgcggc ccggccttcg accagcccgt
      841 cgtgcactcc acgtccctgc ctttcgatgt gcaggaccgg cgtatcgtgc tcgtggacga
      901 tgttctcttt accggccgca ccatccgagc ggcggtcgaa gccctcttcg actacggccg
      961 gccggccgcg gtgcagctgg ccgtgctggt ggaccgaggt caccgcgaac tgcccatccg
     1021 gccggacttc gtgggcaaga acgtccccac gagtcgaacc gaacgggtca acgtcagcct
     1081 cgaggagatc gacggtcgcg acgaggtgac actggagcat ccgcccgata cgcgctagga
     1141 tgagccgacc tttaatcgtg tccagtgagg ccgaaagggg accgacgaat gcatctcatc
     1201 tccatagatg acctctccgt cgccgacatc gagacactgt tccgtctgag cgacgggttt
     1261 ctcgaggtag cgtcgcgccc gatcaagaag gtcccgacgc ttcggggccg cacgcagatc
     1321 aacatgttct tcgaggcgtc cacccgcacc ctcacctcct ttgagctcgc gggcaagcgt
     1381 ctatccgccg atgtcgtcaa catcaaggcg tcgggctcga gcgtggggaa gggggagagt
     1441 ctcaaggaca ccatcctcac tctggagagc tatcgtccag acgtgatggt catacggcac
     1501 gatcgtgtgg gggcgtgccg catggccacg cgctacacga acgcgtgcgt ggtcaacgcg
     1561 ggcgacggct caggcgagca tcccacccag tgccttctcg acctctatag cctgcgcagg
     1621 gagttcggcg ttctggaagg gctgaaggtg gccatcgttg gcgacgtgct gcgcagccgc
     1681 gtggccaggt ccaacgtatt cgggttccgc aagatgggca tcgatgtccg tctggtcggg
     1741 ccacccacct tgatgcctcc gggcatcggc gagtggggtg tgcccaccta cgagaccctg
     1801 gaagccctgg ttgacgtgga cgtcgtctac cttctgcgca tgcagttgga gcgggccaag
     1861 gggagggtgg agccggttcc cagcctccgc gaatacagcc gggtgtgggg attggcgccg
     1921 gccaagctgc gttccggcca gagagtgatg catccgggtc ccatcaaccg cggcgtcgaa
     1981 ctcacgggtg atctcgccga cgacgaccgt tcgctcatcc tgcagcaggt ggagtccggc
     2041 ttggccatgc gtatggccat cctctaccac gtgctggctc ctcacggcga aggggacgcg
     2101 gcaacctagt cagacggggt gaagcccctc tctttgcgtt cctgcgccag tcgcgacctg
     2161 aacacgtggt ccctgacgag gtcggccaac tcggtagggt cgtcgagcag gcagtacttg
     2221 tcgtcgccgt ccttgaccct cagcaccaca caacggtcct cgacgaccac ttcatgcacg
     2281 gtctccagag gcacgacgtc ggggctgtcg gtcgacatcc cgatgagcac cgcatcggtc
     2341 gtgagtctga tgtctccctt ctcccagagc acccgctcct ccgacgagcc cgcctgtatg
     2401 ccgggcttcc aaagatcggc cgtcctggcc agagacgctc cacacgcgcg gcagaatgca
     2461 tcgacctccg cgttctccgc tccacagtcg tagcaggtgt tgcccacgat cgcacctccg
     2521 tccgtcgcat gtatcctaga ggatgtttac ccgtccggcg tattcgtagg cccgcaggac
     2581 ggctgggggc tgcggaggcg gcctgggggc taccggcgga ggcccgcacg gagtaccata
     2641 tgggagcctt taaacgtgtc ctgtgagacc gaaaggaacc tcgatgcatc tgcccgtccc
     2701 cggcggggca ccgcgaatcg cgtgccgcga cctcccattc gagcacctgc tgctccccgg
     2761 cgttcatttg tttgatccga cgcgcggcct ggacgagcgc gggtcgctcg tggtgcgcga
     2821 cggggtcatc gtcgctctgg gtgcgatctc cgagcctccg cccggggcca ccgtcctgtc
     2881 ggaggtggcc gggtgccacg tgttccccgg gttcgtcgac atgcatactc acttgcgcac
     2941 cccgggacag gagtacaagg aggatctcga gtccgccggc cgcgccgcgg cggcgggcgg
     3001 ctttgtgatg gtcacgggaa tggcgaatac cgaccctgtc gtggacagcg ggccgctggc
     3061 cacctgggtg ctcgatcaag cggagagcag ggcggcggtg gccgtggccc acgtgggagc
     3121 ggtgagcaag ggttcgcgag gcggatccct ggccgagctg agggaactgg tggacgcggg
     3181 agtggtcgcc ctgtccgatg acggacgccc cctcgaagac atggacctgc tgctggcggc
     3241 gctccgctac ctgcggatca cgcggcggcc gctgctcctt catctggagg acctcactct
     3301 ctcgaccgac gcggccatgc acgagggtgc gtggagcgcg cgcctgggct tgcggggtgt
     3361 atcaggcgcg gccgaggcgg gcccgatggc tcgggatctc gaggtcctga gggtggtcat
     3421 ggctgaggag tcggcccgcg acgcaggccg ggccggcgcc ggcggagcgc ccgcgccgcg
     3481 cctgcatttc cagcacctgt cgaccgaggc ggcggtgcgt ctcttgaggg cggccaaggc
     3541 tgacgggctg cctgtgacgg cggaggtgac gccccacaac ctcgtgttca ccgatgagcg
     3601 ggtggcgtcc ttcgagcaga gctgcaaggt gaacccgccg cttcggtcgg aacgcgaccg
     3661 cgctgcggtc gcggccgctc tcgcggaggg gatcatcgat tgcgtggcca ccgaccacgc
     3721 cccccatgcg tcgcacgaga aggaggtacc cttcgaagac gcgccgtcgg gcaccatcgg
     3781 cctcgagacc gcgttcgcgg cgatctacgg gggtctggtg gcgtcgggcg ggctgaccct
     3841 cgagaggttg atcgacgcgc tctccgcagc accctgtcgt tgccttggga tcgacccacc
     3901 tcggctggag gtcggtgctc ccgccgatct atgcgccgtc gacctgactg aggagtggac
     3961 ggtcggcgtc gctgacctgc acagcaagag tcggaactcc gccttcttgg gggagcgcgt
     4021 ccaaggacgc gtgcggttga ccgtggtacg cggcgcccgc cggttcgagc gcggacgtcc
     4081 ctgagtctga gccctgagat ctgagcccac gcggagtcca tgaggagacc tggcagtgta
     4141 cagaagtgag atccccggat atgtggtgtt agaggacggg accgtgttcc gaggcttcgg
     4201 attcgccgtc gaagggcggg tcctcggaga gatcgtgttc aacacgggga tgaccggcta
     4261 tcaggaagtg accaccgatc cttcttacca cggtcagatg gtcactttcg cctatcccat
     4321 gatcggcaac tacggtgccg cggatgccct gctcgaatcc gccgatgtgc actcgcgggc
     4381 gatcgtcgtg cgcgaggtga agaacaccaa ctacaatcgc acgtgtccaa ccgcgtgggt
     4441 cgactggctg cacggcgcct ccgtggtggg cgtgggcggg gtggacaccc gcgcgctcac
     4501 ccgtcgaatc cgggagcacg gcgcgatgaa cgcctgcgtt gccgcaggtg cgcacctgaa
     4561 ggtggacgag ctgcttgagg aggtgcgggc ccagccgtcc ctctcgggcc gcgacttggt
     4621 tcgggaagtc acatgctccg agccatatgt ggtcggctcc gcgaacgcgc ggcatcacgt
     4681 cgtggcatac gacttcggca tgaagcgctc catcctcagg aacctcctcg aggcgggctg
     4741 tcgggtgacg gtggtgcctg ccggttggac ggcggcgcaa gtgctggaac tggagcccga
     4801 cggggtcttc ttgtcgaacg gtcccggcga cccacaaccg gtcacctatg ccgtcgaggc
     4861 gatcagggaa ctcctcggcc gcgttcccgt cttcggcatc tgtctgggcc accagctgat
     4921 cgcgctggcc ttgggcctga ccacctacaa gctgaagttc ggccaccgag gcataaacca
     4981 cccggtgaag aactacctct cggactccat cgagatcacc agccagaatc acggcttcgc
     5041 ggtcgccccg ccgcccgcag tcgaacgagc gtgggccgag ggcaccgatc tgtcgtccat
     5101 cccggctgca gacatggtgg ttgagtccga tctcggccga gtgcaggtga cccatctgaa
     5161 cctgaatgac ggcacggtgg aaggccttcg cctcctggac aggcctgcgt tctgcgtgca
     5221 gtaccatccc gaagcgagcc ccggccccca tgacagcctc tacctcttcc gagagttcac
     5281 agagtcgatg cggcgttccg gccgcgtcca ggccgggcgg ctgtaacgtg tctgcggaag
     5341 gcgcggagcg ttcggaacgg gctgaacaca cagagtgggt agaacgggca gacctccttt
     5401 ctgctcggat cgagtcctat cgcgacgaga tggtcgcgtt ccagaggtct ctcaccgcta
     5461 tccgcgcgct cggaccggag aacgggggag agggagagtg ggaaaggtcc cgcttcctgc
     5521 tggaccaggc acgacgcttc ggcttcaccg acatcgtgga agtcgacgcc gaggacgagc
     5581 gcgtgccggg aggccgtcgc cccaatctca tcctccgcgt gccgggccga tcggcgcacc
     5641 ccgcggtgtg ggtgatggcg cacatggaca ctgtgccccc gggcgagacc gcactctggg
     5701 atagcgaccc tttcgtggcc gaagtttgcg atgggcgcat cgtcgggaga ggcgtcgagg
     5761 acaatcagca ggggctcgtt gccgggctct tcgcgctgag ggctctgctc gacgaaggcg
     5821 tgactcctac caccgacgtg aggctgctgc tggtggccga cgaagagacg ggcaacgcac
     5881 gcggcgtgga ccacgtcctg gagactcgac ctgcgctgtt cgacccgtcg gacctggtga
     5941 tcgtgcccga cgctggcgcg cccgacggcg ccgtattgga gatcgccgag aagtcggcgc
     6001 tgtggatccg cctggcggtg cggggcaagc aggtgcacgc ctccgtgccg caggccgggg
     6061 tgaacgcgca cagggcggcg gcgcacctcg tggtgcgcct cgaccggcgg ctccaccagg
     6121 tcttcgacgc cgaggacgga ctgttcgagc cgcccggctc cactttcgag ccgaccaagc
     6181 gcgaagccaa cgtgcagaac gtcaacacca tcccgggtga ggacgtcctc tacttcgact
     6241 gccgaatact cccaagatac gggttggcgg ccgtcaaggc ggaggtcgag gaggaggccc
     6301 gagagatcgc ggccgagttc ggggtgcgcg tggacgtctc ctaccccac
//
LOCUS       DASRMJ010000291         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_62267, whole genome shotgun sequence.
ACCESSION   DASRMJ010000291 DASRMJ010000000
VERSION     DASRMJ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62267"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..952)
                     /gene="ftcD"
                     /locus_tag="VFD74_08030"
     CDS             complement(68..952)
                     /gene="ftcD"
                     /locus_tag="VFD74_08030"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HZK49532.1"
                     /translation="MKKLMCVPNVSEGRDAAVVERLADSLRNDPGVKLIDLSSDRDHN
                     RSVYSYLGDSEAVLAATERLAGIVFETVDMRTHAGRHPRMGALDVVPFIPVGTTTTEE
                     AVDIARRFGRFVGEKGIPVYYYEDAATSPRRTSLVDVRKGEYEGLEAKLLDPEWAPDE
                     GPAIFNAATGSVQVAARFPLVAFNVNLNTDDLAVATRIAKAVRQRDGGYRYVRAMGLV
                     LEGTGMVQVSMNLTNYEGTPIPRVLETIRSEAARHGVSVAGAELVGPVPVKALEEVVK
                     QYLQVHAFSVEQIIEMNV"
     gene            complement(1002..>2042)
                     /locus_tag="VFD74_08035"
     CDS             complement(1002..>2042)
                     /locus_tag="VFD74_08035"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK49533.1"
                     /translation="ALRMTVSDPSSLGDMLGARGIRSGPMATVERTRRSSAAELVESA
                     AARLAGMLVTGTTTVESKSGYGLSTASEVLLLEVNRALDANSPVDVVSTFLGAHGFPG
                     EVARGDYLRCVIDEMIPLVAAAGLAEFADVWCGDGYYTAAETRTILQAARYAGMEPKI
                     HADAYSYIGGSDVGAEMGAVSVDHLNYTPRPVVRRLAEAGVVGVIMPALDFAVRHPRP
                     FDARAMIEEGMTLALATDLCPACWVESQQFVMALACRNYAMSPAEALWAATRGGALAL
                     GMRDRGVLAAGCLADIQIWGVPTFEHVIYRLGGNVVDQVLKRGRVVVDRGASVGGRVV
                     VGPGAPAGGRVA"
BASE COUNT          328 a          731 c          670 g          313 t
ORIGIN      
        1 tacgtagctc ggcgagtgcg gcgaaccgtc acggggggcc gcccgcgggc ccggcagagt
       61 gccgtgatca cacgttcatc tcgatgatct gctccaccga gaacgcgtgc acctgcagat
      121 actgcttcac cacttcctcc agagctttca ccgggaccgg gcccaccagc tccgctcccg
      181 ccaccgacac cccgtgtcga gcggcttcgg aacggatcgt ctcgagcacg cgggggatcg
      241 gggtgccttc gtagttggtg aggttcatgg atacctgcac catgccggtg ccctccagga
      301 ccagacccat ggcccgcacg tagcgatagc cgccgtcgcg ctgccgaacg gccttcgcga
      361 tgcgcgtggc cacggcaagg tcgtcggtgt tcaggttgac gttgaaggcg acgaggggga
      421 agcgggcggc cacctgcacg ctgcccgtgg ctgcgttgaa gatcgccgga ccttcatcgg
      481 gtgcccactc cggatctaga agcttggcct ccaggccctc gtactcgccc ttgcgcacat
      541 ccaccagact cgtgcgccgg ggcgaggtgg ccgcgtcctc gtagtagtag acggggatgc
      601 ccttctctcc cacgaaccgc ccgaagcgac gggcgatgtc caccgcctcc tccgtagtgg
      661 tggtccccac cgggatgaac ggcaccacgt cgagggctcc catccgagga tggcgccccg
      721 cgtgagtgcg catatccacc gtctcgaaga cgatcccggc cagtcgctcg gtggcggcca
      781 gcaccgcttc ggaatcgccc aagtacgagt agaccgagcg gttgtggtct ctgtccgaag
      841 agagatcgat gagcttgacg ccggggtcgt tccttagtga gtccgccaag cgttcgacga
      901 ctgccgcgtc gcggccctcg ctcacgttgg gcacacacat gagcttcttc acggtccctc
      961 ctctgtatgg gcggatgcgg gcctgagcgg caagaggggc ctcaagcgac ccgccctccg
     1021 gcgggggcgc cagggccgac gaccacccgt cctccgacgg aggcgccacg gtccacgacg
     1081 acccgccccc tcttcagcac ctgatccacg acgttgccgc ccagtcgata tatcacgtgc
     1141 tcgaaggtgg ggacacccca tatctggatg tcggcgaggc acccggcggc gaggactccc
     1201 cggtcgcgca tgcccagcgc gagggccccg ccccgcgtgg cggcccacag ggcctcggcg
     1261 gggctcatgg catagttgcg gcaggccagg gccatcacga actgctgcga ctcgacccag
     1321 cacgccgggc acaagtcggt ggccagagcc agcgtcatac cctcctcgat catggcgcgc
     1381 gcgtcaaagg gtcgagggtg ccgcacggcg aagtcgaggg cgggcataat cactcccacc
     1441 actcccgcct ccgccagacg ccgcaccacc gggcgtggcg tgtagttcag gtggtccacc
     1501 gataccgctc ccatctcggc gcccacgtcg gaccccccga tgtaggagta ggcgtcggcg
     1561 tggatcttgg gctccatccc agcatagcgc gccgcctgaa ggatggtgcg ggtctcggcc
     1621 gcggtgtagt agccgtcgcc gcaccagaca tcggcgaact ccgccaggcc cgcagccgcc
     1681 acgagcggga tcatctcgtc gatgacgcac cgcagatagt caccccgggc gacctcgccg
     1741 gggaagccgt gggccccgag gaaggtggag accacgtcca cggggctgtt cgcgtcgaga
     1801 gcccggttga cctcgagcag caggacctcg gaggccgtcg agagtccgta gccgctcttg
     1861 ctctcgacgg tggtggtgcc ggtcacaagc atcccggcca gtcgggcggc ggcagactcg
     1921 accaactcgg ccgccgacga tcgccgcgtg cgctccacgg tggccatcgg tccgctgcgg
     1981 ataccccgcg cccccagcat gtcccccagg gaactcgggt cgctcacggt catgcgcagc
     2041 gc
//
LOCUS       DASRMJ010000292         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_107611, whole genome shotgun sequence.
ACCESSION   DASRMJ010000292 DASRMJ010000000
VERSION     DASRMJ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107611"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFD74_08040"
     CDS             <1..327
                     /locus_tag="VFD74_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZK49534.1"
                     /translation="VAEDVIATYVAAAVRSIPGVSELHSNPWQELSGRVHLGPQRKGV
                     VVREVAPGAVEVEVHVSVAWDTVIPEMAQQLQETVSHNVETLLDLQVRKTTVYVDEVE
                     APLELR"
     gene            455..940
                     /locus_tag="VFD74_08045"
     CDS             455..940
                     /locus_tag="VFD74_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49535.1"
                     /translation="MRALETGWGSVGLVALEDHETIASIQFAPIRSLARPHTQPLGQP
                     PEEAVLLFCLRGRVGRPNIETQKLLHQTMSHLRRRGVREVYAYARPLDTHSLSGVRNL
                     FGLEFLEDNGFGVARALDHAYLMRADLGGLVPTLAEAGWSIRRRLGAGARPSPATFGR
                     P"
     gene            943..>2004
                     /locus_tag="VFD74_08050"
     CDS             943..>2004
                     /locus_tag="VFD74_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HZK49536.1"
                     /translation="MKRPPPHRLFLGLALLVLLAASLGPHPESAAGANGSPAPVWVTR
                     VEGAIVPPLSAYLVGTMERAREAGAEALVIEIDTPGGLDSSMREIIQAEIDSSMPVVI
                     YVYPQGARSASAGVYIMMGADVAAMAPQTNLGSAHPVSLTGQMDEEMTKKVTNDAAAY
                     IRGLAATHGRNTTWVEQAVRESVSLTAEEAKAQNVIEFVAEDLEDLLAQMDGHTTTPK
                     GVTLHTAGAPVVEVSMSWREKFLHRLVDPNIIFILMLLGVYGLIFEFQNPGLGAPGIA
                     GAISLLLAAYGLQILPVNYVGLLLLAVAMVLYVAEVKIQSSGVLALGGTVALVLGGLL
                     LFNAPGSVIRVDWTVIAIMA"
BASE COUNT          340 a          667 c          659 g          338 t
ORIGIN      
        1 gtggcggagg acgtgatcgc cacctacgta gccgcggccg tgcgcagcat tccgggtgtg
       61 agcgaactcc acagcaatcc gtggcaggag ctatccggac gtgtccactt gggcccgcag
      121 cgcaagggcg tcgtcgtcag agaggtggcg ccgggggccg tggaggtcga ggtccacgtg
      181 tcggtagcct gggacaccgt gatacccgag atggcacaac agctccagga gaccgtctca
      241 cacaacgtcg agactctgct cgatctccag gttcgcaaga ccaccgtgta cgtggacgag
      301 gtcgaggcac ctctggagct gcggtgagac cgtggccgcg tacgtagaag tggtagccgc
      361 gactcaggga ctccttcccc ctccttgccc ggactgcctg tggtggcaaa cgacccaggg
      421 tcctacgcaa ggcaggcagc gcaggatcga gtggatgcgc gcgctggaaa cgggctgggg
      481 gagcgtgggt ctggtggccc tagaagacca cgagacgatc gcctccatcc aattcgcccc
      541 catccgctcg ctcgcgcgtc cgcacacgca gccgttgggc caacctcccg aggaggcggt
      601 gctgctcttc tgcctgcggg gacgcgtggg tagaccgaac atcgagaccc agaagctcct
      661 tcaccagacg atgtctcacc tgcggcgtcg cggtgtgcgt gaggtctacg cgtacgcccg
      721 ccctctggac acgcacagcc tcagcggtgt gcgcaacctc ttcgggctcg agttcctcga
      781 ggacaacggt ttcggggtgg cgcgcgcgct cgaccacgca tacctcatgc gcgcggactt
      841 gggcggactc gttccgacgc tcgccgaagc cggctggtcg atccgcaggc ggctcggagc
      901 aggagcacgc ccgagtccgg ctaccttcgg tcgtccctga cgatgaagcg cccaccccct
      961 caccgcctct tcctggggtt ggctctgctg gtgcttctcg cggcctctct gggcccgcat
     1021 ccggagagtg ccgccggcgc gaacggaagt cctgcgcccg tctgggtcac gcgggtggag
     1081 ggagccatcg tgccgcccct ctccgcctac ctcgtgggca cgatggaacg ggcgcgcgaa
     1141 gccggcgccg aagccctggt catcgagatc gacacccccg gcggcctgga cagctcgatg
     1201 cgagagatca tccaggccga gatcgactct tccatgccgg tggtgatcta cgtctatcca
     1261 cagggagcgc gctcggcctc ggccggcgtc tacatcatga tgggggccga cgtggccgcc
     1321 atggcacccc agaccaacct cggctccgcc caccccgtgt cgctcaccgg gcagatggac
     1381 gaagagatga cgaagaaggt caccaacgac gcggccgcct acatccgagg actggcggcc
     1441 acgcacggtc ggaataccac gtgggtcgag caggccgtgc gcgagtccgt gtccttgacg
     1501 gccgaggagg ccaaggcgca aaacgtcatc gagttcgtgg ccgaagacct cgaggacctc
     1561 ctggcgcaga tggacggcca caccaccaca cccaaaggcg tgacgctgca caccgccgga
     1621 gctcccgtcg tcgaagtctc gatgagctgg agagagaagt tcctgcaccg cctggtcgat
     1681 cccaacatca tcttcatcct tatgctcttg ggcgtctacg gactcatctt cgagttccag
     1741 aaccccgggc tcggcgcgcc gggcatcgcg ggcgccatat cgctcctgct cgccgcatac
     1801 gggctgcaga tccttcccgt gaactacgta ggtctgctgc tgctggcggt ggcaatggta
     1861 ctctacgtgg cggaagtgaa gattcagagc agcggcgtgc tcgccctcgg gggcaccgtc
     1921 gctctggtcc tcggtggatt gttgctattc aacgctccgg gatcggtgat tcgcgtggat
     1981 tggacggtca tagccatcat ggcg
//
LOCUS       DASRMJ010000293         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_107751, whole genome shotgun sequence.
ACCESSION   DASRMJ010000293 DASRMJ010000000
VERSION     DASRMJ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107751"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFD74_08055"
     CDS             <1..230
                     /locus_tag="VFD74_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZK49537.1"
                     /translation="RTLVNGTVRQEGRVCDMISDPFTIVSFVSQAMTLLPGDLLMLGT
                     PPGVGELHPGDSVVVDIDGLEPLQNPVVAEE"
     gene            581..4078
                     /gene="dnaE"
                     /locus_tag="VFD74_08060"
     CDS             581..4078
                     /gene="dnaE"
                     /locus_tag="VFD74_08060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZK49538.1"
                     /translation="MDPSKVTSEPVGRASFCHLHVHSDFSALDGACKIDDLVAKAVEN
                     GMPAVAVTDHGVLSGAVQLYRKATAAGVKPIIGLEAYVVEDRFHKEDGHREERWHLTL
                     LARDNAGYRNLLGLSSKSFLEGYYTKPRADYALLREHAEGLICLSGCPTGRLSKALEQ
                     GSDRDADAEIRRLIDIFGRDNLYIEIQVTGIEELAHVPARLAELAARVDIPLVATNDI
                     HYLEASDAAAHDALLCVQTGSRLEEEKRMRFSSEEFYFKTEAQMLEAFHEYPEAVSNT
                     VLVAERCNVTMDFDRILLPQYTVPDGFPGEPAYLRHLCEQGLTRRYVGEPSTEVRERL
                     EMELRVIEEMGFPPYFLIVWDFVTFAKRSGIPVGPGRGSAAGSLVSYLLGITDLDPLE
                     YDLLFERFLNPARISMPDIDIDFSVEGRERVIDYVAQKYGRDRVAQIATFGTIKARQA
                     IRDAARVMDVAYNVADRIAKLIPEGPKATLDDCMRDKQDLRIEYDADDTVRRVVDVAR
                     TLEGLIRQDSIHAAGVVISDKPLTDYLPLMQKGDAEVVTQVPMGDVEKLGLLKMDFLG
                     LRNLDVLAGAEKIIRESTHPEFDLETLPLDNAGTYRMLAAGDSEGVFQFESSGMREAL
                     REVGPTRFEDLIALVALYRPGPMEYIPQYARNKRNPEGITFPDERLEPILRPTYGVAI
                     YQEQLMEIAKRIGGFSPAEADDLRKGIGKKIRAVLDRLEPQFRAGALAGGAKKSVVDF
                     LWSLMEKAGDYSFNKSHAACYALIAYRTAYLKANFPVEYMAALISSVMNTKDKVPYYV
                     GVANDMGIEVLPPDVNESALDFRVVEGRIRFGLNAVKNVGEGAIRLIIAARESGGPFR
                     DIFDFCERVDPSVVNKRALESLIKAGGLDSTGSSRKGMLHVLAQAMANGQKRLADDAA
                     GQGSIFDLMGSPRGAVDSSGAETRRAQPVPIPEEDFTKDELLRLEKETLGIFLTSHPL
                     RDLRRQIRDEAEHMISELSGLSDGHVTTIVGMVARVKRVPTKSGDQMAFVIIQGIEDS
                     IELTCFPKLYSDSRELLEEDRLVKIRGRVERREESEAKFIPFSIERFTPKTGTEPVCI
                     KLDAIAVQRDILDDLKSILANFPGPCPVELHLDTDKGCVKMRCGRGYQVDPQTSLFAE
                     LRVLLGEAGVFRYDPALSA"
     gene            4267..>4933
                     /locus_tag="VFD74_08065"
     CDS             4267..>4933
                     /locus_tag="VFD74_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HZK49539.1"
                     /translation="MGIYDSEVIVTVLRTPPGTKDVLPTEAAELRLIEDAVRTVFHEF
                     GYGEVITPALEYEEVLALSEEKAFLTGFRLLDESGKVLILRPDLTTPIARLVGSRLRA
                     GETPHRVFSISDVFRRNNPQRGEENEFRQAGVEFLGSSLPQADAEVLAVACRALERAG
                     LEDFRVGVGQVRFFQALLDAVTDDPALKVRLTTALVDKDMVGYRVAVEQADLPAGDRA
                     ALLE"
BASE COUNT          854 a         1608 c         1628 g          843 t
ORIGIN      
        1 tccgtacgtt ggtcaacggc accgtccgcc aagaggggag ggtgtgcgac atgatcagcg
       61 atcccttcac gatcgtctcc ttcgtcagcc aggccatgac gcttcttccg ggtgatctcc
      121 tcatgctggg gacgccgccc ggagtgggcg aactccaccc cggcgactcg gtggtcgtgg
      181 atatcgacgg gctggagccc ctacagaacc cggtcgtcgc ggaggagtag ggcggccacc
      241 aactgcgccg gctactacgg cgccctcgcc cgaggtttcg gccacctgcc gcttccgacc
      301 gttcacgggt ccgcaaggac ctcgtgttcg ctttccgcag cgcctgctca ggggcccacg
      361 gaccgcctat ccgcccgcac cggcgtgttg ctgtgagcgg acgaaatacc gctggtagcc
      421 aacctggtgc ttgaggccgc gggggggttt cgggtagaat cgtggggcgc gtcggaggct
      481 cggcacgatc cggccgcgtc gcattctctg ccctggcagg aatcccgtgt gtgggcaggc
      541 gttccccgcg cggcgcccga cgagaggaac cgaacagtct gtggatccaa gtaaggtgac
      601 gagcgagccg gtgggcaggg catccttctg ccacctgcac gtgcactccg atttctcggc
      661 gctcgacggt gcctgcaaga tagacgacct ggtggccaaa gcggtggaga acggcatgcc
      721 cgctgtggcc gtgaccgacc acggcgtcct gagcggcgcc gtgcagctct accgcaaagc
      781 tacggccgcg ggagtgaaac cgatcatcgg gctcgaggcc tacgtggtcg aagaccgctt
      841 ccacaaggag gacgggcacc gcgaagagcg ctggcacctc accctcctcg ctcgggacaa
      901 cgcgggatat cggaacctcc tgggactctc gagcaagtcc ttcctggagg gctactacac
      961 gaaaccgcgg gcggactatg cgctccttcg ggagcatgcc gaggggttga tctgtctctc
     1021 gggctgtccc accggccgcc tctcgaaggc gctcgaacag gggagcgatc gggacgccga
     1081 cgctgagatc cggcggctca tcgacatctt cggccgcgac aacctgtaca tcgagatcca
     1141 ggtcaccggg atcgaggagc tcgcgcacgt gcccgcccgg ctggcggagc tggccgctcg
     1201 ggtagacatc ccactggtgg ccaccaacga tatccactac ctcgaagcct ccgatgccgc
     1261 cgcccacgac gcgctgctgt gcgtgcagac gggttcgcgc ctggaggagg agaagcgcat
     1321 gcgcttctcc tccgaagagt tctacttcaa gaccgaagcc cagatgctcg aggcgtttca
     1381 cgagtacccg gaagccgtca gcaacaccgt gctggtggcg gagcgctgca atgtgaccat
     1441 ggacttcgac cgcatcctcc tgccgcagta cacggtgccc gatggtttcc ccggagagcc
     1501 ggcctacctg cgccacctgt gtgagcaggg gttgactcgc cgctacgtag gagagccgtc
     1561 cacagaagtg cgtgagcgtc tggagatgga gctccgagtc atcgaggaga tgggcttccc
     1621 gccctacttc ctcatagtct gggacttcgt cacattcgcc aaacggtcgg gtatcccggt
     1681 gggaccgggc cgcggctcgg cggcgggttc cttggtctca tacctgctcg gtatcaccga
     1741 tctcgatccc ctcgagtacg acctgctctt cgaacgcttc ctgaaccccg cacgtatcag
     1801 catgcccgac atcgacatcg acttctcggt ggaagggcgg gagagagtga tcgactacgt
     1861 ggcccagaag tacgggcgcg accgcgtagc gcagatcgcc accttcggca ccatcaaggc
     1921 ccgccaggcc atccgcgacg ccgcgcgcgt catggatgtc gcctacaacg tcgccgaccg
     1981 catcgcgaag ctcatcccgg aggggccgaa ggccaccctt gatgactgta tgcgcgacaa
     2041 gcaggacctg cgcatcgagt acgacgcgga cgacaccgtg cgccgagtgg tcgacgtggc
     2101 gcgcaccttg gaggggttga tccgtcagga ctccatccat gccgccggcg tggtcatctc
     2161 cgacaagccg ctcaccgact acctgccgct catgcagaag ggcgacgccg aggtcgtgac
     2221 gcaggtgccg atgggcgacg tggagaaact cggtctgctc aagatggact tcctgggtct
     2281 gcgcaacctg gatgtgctgg ccggtgccga gaagatcatc agagagagca cgcatcccga
     2341 gttcgatctg gagaccctgc cgctcgataa cgcgggcaca tatcgcatgc tcgcggctgg
     2401 tgactccgaa ggcgtcttcc aattcgagtc gagcggcatg cgcgaagctt tgcgcgaagt
     2461 gggccccacg cgcttcgaag atctgatcgc tctcgtcgcc ctctaccggc cggggccgat
     2521 ggagtacatc ccccagtacg cgcgcaacaa gcgcaatccc gagggcatca cgttccccga
     2581 cgagcggctg gagccgatcc tgcgtcccac ctacggggtg gccatctacc aagaacagct
     2641 gatggagatc gcgaaacgca tcggcggatt ctccccggcc gaagccgacg acctgcgcaa
     2701 gggcatcggc aagaagatcc gagcggtcct cgataggctc gagccgcagt tccgcgcggg
     2761 agcgctggcc ggcggcgcaa agaagtcggt ggtcgacttc ctctggtcgc tcatggagaa
     2821 ggcgggcgac tactccttca acaagagcca cgccgcctgc tacgcgctca tcgcctaccg
     2881 caccgcctac ctcaaggcga acttcccggt cgagtacatg gccgctctca tctcgagcgt
     2941 catgaacacc aaagacaaag tcccctacta cgtgggcgtc gccaacgaca tgggcatcga
     3001 ggtgctgccc cccgacgtga acgagtcggc gctggacttc cgagtggtgg aggggcgcat
     3061 ccgcttcggt ctgaacgccg tgaagaacgt gggcgaaggc gccatcagac tgatcatcgc
     3121 ggcgcgggag agcggggggc cgttccggga catcttcgat ttctgcgagc gtgtcgaccc
     3181 gagcgtggtc aacaagcggg cgctcgagag tcttatcaag gcgggcgggc tcgattctac
     3241 gggttcgtcg cgcaagggca tgctccatgt cctggctcag gccatggcca atgggcagaa
     3301 gcggctcgcc gacgacgccg cggggcaagg ctcgatcttc gacctgatgg gctcgcctcg
     3361 tggtgcggtc gacagttcgg gggccgagac ccggcgtgct cagccggtgc ccatccccga
     3421 ggaggacttc acgaaggacg aactgctgcg tctcgagaag gagacgttgg gcatctttct
     3481 cacctcccat ccgttgcgcg acctgcgccg ccagatccgc gacgaagccg aacacatgat
     3541 ctcggagctc agcggactca gcgacggcca cgtgaccacg atcgtcggca tggtggcgag
     3601 ggtgaagagg gttcccacca agtcgggaga ccagatggcg tttgtgatca tccagggcat
     3661 cgaggactcc atcgaactca cctgcttccc caagctctac tccgactcgc gcgaactgct
     3721 cgaagaggat cgcctggtga agatcagggg cagggtggag cggcgcgagg agagcgaggc
     3781 gaagttcatc cccttctcca tcgaacgctt cacccccaag acggggaccg agccggtgtg
     3841 catcaagctg gatgctatcg ccgtgcagcg tgacatcctt gacgatctga agtccatcct
     3901 tgcgaacttc cccggcccct gcccggtcga gcttcacctc gacaccgaca agggttgcgt
     3961 gaagatgcgc tgcggcaggg gctatcaggt ggatccgcag accagcctgt tcgccgagtt
     4021 gcgcgtgctg cttggcgaag ccggcgtctt ccgctacgat cccgcgctga gcgcgtagga
     4081 gtcggtctcg ggctcggcgt cgcgctgggc gggctctctt ccgcgccagg gagcgccgcg
     4141 ccgggcgggc gccacattgg gccgggcggg ctccgtgccg cggcgaggag tgtcacggtt
     4201 cgcccccggt ctttccgccc atccgcgcgc cgtggatggg gtcatgtgcc cgctcggtgc
     4261 gctactatgg gcatctacga ttcggaggtg atcgtgacgg tcttgcgcac tccccctggc
     4321 accaaggatg tcctgcccac cgaagccgcg gaacttcggc tcatcgagga tgccgttcgc
     4381 acggtattcc acgaattcgg ctacggagag gtcatcacgc ccgcgttgga gtacgaggaa
     4441 gtgctggcgc tctccgagga gaaggccttc ctcacgggct tccggctgct cgacgagtcg
     4501 ggcaaggtgc tgatcctgcg tcccgatctc accacgccca tcgcccgtct ggtgggcagt
     4561 cgtctgaggg cgggggagac cccgcatcga gtcttctcca tctccgacgt ctttcggcgc
     4621 aacaacccgc agcgcggcga ggagaacgag tttcggcagg cgggtgtgga gttcttgggt
     4681 tcgtcactgc cgcaggcgga tgccgaggtg ctcgcggtgg cgtgccgcgc gctggagcgc
     4741 gcggggctcg aggacttccg cgtgggcgtg ggccaggtcc gtttcttcca agccctgctc
     4801 gatgccgtca ccgacgaccc agccctcaag gtgcggctga cgacggccct ggtggacaag
     4861 gacatggtgg gctaccgagt ggccgtcgag caggccgatc tgcccgccgg cgatcgagcc
     4921 gcgttgctcg agg
//
LOCUS       DASRMJ010000294         5891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_107802, whole genome shotgun sequence.
ACCESSION   DASRMJ010000294 DASRMJ010000000
VERSION     DASRMJ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107802"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..825)
                     /gene="nth"
                     /locus_tag="VFD74_08070"
     CDS             complement(112..825)
                     /gene="nth"
                     /locus_tag="VFD74_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006883336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZK49540.1"
                     /translation="MVSPSTPDGAPQGGPTVRRGPKPWRAAQAAEVLSRLGREYPDAG
                     IMLVFGNEWELLVSVILSAQCTDVMVNKVTADLFKKYRTLEEYAGADAAEFEADIRPT
                     GFFRNKTKHILGAAQVVLRDFDGRVPHTIAGLLTLLGVARKTANVVLGNMDPAAYSSD
                     PDAGIAVDTHVHRLSNRLGLAKSTDAAATETDLVSVVPRDDWFRITYLLIEHGRAVCV
                     ARKPRCGACLLTDICPSAA"
     gene            complement(1006..1635)
                     /locus_tag="VFD74_08075"
     CDS             complement(1006..1635)
                     /locus_tag="VFD74_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HZK49541.1"
                     /translation="MTQNTQTQTAPTRPALAVNTQTTNVLLSGVGGQGVLLASYVLSQ
                     AAMAEGYDVKQSEVHGMSQRGGSVVSHLRFGAKVAAPIVTMGAADLLLSFEPLEALRY
                     HHWLRPGGLLIYNALRVNPSTVAAGLATYPDEIDARIAEIWPNSRKVDATALAEQAGT
                     AKAANMVLLGAISGRLPFADETWRKIIAEVVPPKTIDANMRAFDLGRDS"
     gene            complement(1647..3437)
                     /gene="iorA"
                     /locus_tag="VFD74_08080"
     CDS             complement(1647..3437)
                     /gene="iorA"
                     /locus_tag="VFD74_08080"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HZK49542.1"
                     /translation="MRELLSGNEAIARGAWEAGVRLAAAYPGTPSTEILQNMAEYKEI
                     ESQWSPNEKVATEVALGASIAGARSLAVMKHVGVNVAADPLFTASYTGVGGGFVLVTA
                     DDPAMHSSQNEQDNRNYARFAKIPMFEPSDSQEAKDLVRVAFEISEEFDTPVLFRTTT
                     RISHSKSIVELGERVELPALTTLEKDVPKYTMLPGFAKLKHPKVEQRITDLKKRAETL
                     PVNRIEMGDSAVGIIATGTVYQYAREAFPQASFLKLGMTYPLPRKMIEEFRSKVERLY
                     VVEELDPFLEEQIYLMDVKLDGGKDLFSLCGELDPGAVARGLTAGGATGAEPTLLLPP
                     PAEAAGLPQRPPTFCPGCSHRGVFTVLKKLRVFVSGDIGCYTLGALPPIGAMHTCVCM
                     GASIGMAHGMSKVVDAQGDIKNRAVAVIGDSTFFHSGVTGLMDTIWNRGASVTIILDN
                     RTTAMTGGQQNPGSGNTLDGEPASMVDLTKLVEALGVKRVARIDPYDLAQTERVIREE
                     LEAGEPSVIIATAPCVLEFKIKNDPWTIDENLCNGCKRCLRVGCIALNLVEEGEKTLV
                     EIDPIQCNGCGVCAQMCNFDAILAPEPVKA"
     gene            3780..4928
                     /locus_tag="VFD74_08085"
     CDS             3780..4928
                     /locus_tag="VFD74_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZK49543.1"
                     /translation="MRRDVAVSPRTGNEHEFVVLESADWVAVVALTEDDRLVLVRQFR
                     HGLREVTLELPGGLVDAGMSPEEAARAELRQETGYGGGEWAELGRLAPLPAVFTNHVH
                     VFLARGVRLLGDPEPDPGEDIVTDLVDAEDARRMVERGEITHAPVVAALFLWDLEARS
                     GGSSAVTTHVLDAPTPGEETPGEDIGSLRDRILAQLRSHFTLTVATVGSVGVESRPVD
                     AAAAGGADEGAEPGDADEPHDADEPGDADEPHGADETRAPHAATVFYAVDRRGRLVFL
                     SKGDSRHGRHIGDGGPVAATVAREYADWREIQGVQVWGRAEPLHGAARVGALAVYLAR
                     FPFVSALLEDPRLAARLRGIEVYRITPRSAALTDNQLGPFGREVLEDL"
     gene            4991..>5891
                     /locus_tag="VFD74_08090"
     CDS             4991..>5891
                     /locus_tag="VFD74_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HZK49544.1"
                     /translation="MTARWVRDALTRVLGGEPFPGTLNLRLEPGPALESWRALKASAS
                     GVQLPPGDEGSCASTLFPVSVEGTVRGVLLLPHVEGYPEDMVELVARESLRERFRLGD
                     GSVVALSWGSAAMKDRQLEACLSGAVESSRGNGFAAFELEGDLPDFDLAALDTSTRVF
                     GRRLSLPLFVSSMTGGGVGSGLINRRLAEAAQATGIGMAVGSQRLMLEDSSLVSDFRV
                     RDWAPDILLFADLGLVHLNRGLTRELCLRAVEEIEADALMLYVNPLHEALQAGGDLDF
                     AGLLDRLSDLCRDFPYPILLKEVG"
BASE COUNT          869 a         1867 c         2052 g         1103 t
ORIGIN      
        1 gctacggcct aggtggcgcg atctcagggg tattggctgg tcgttcaatc aagatggtat
       61 cggtcgctcc gaggggagtc aagaagcgca cgggcggggg gacgtcgacg cctacgccgc
      121 cgaggggcag atgtcggtca ggaggcacgc cccacagcga ggctttcggg cgacgcagac
      181 cgcccgccca tgctctatca agaggtaggt gatgcggaac cagtcgtctc gggggaccac
      241 cgacaccagg tcggtctcgg tcgcagctgc gtcggtgctc ttcgcgaggc cgagtcggtt
      301 cgacaagcgg tgcacgtggg tgtcgacggc gatgccggcg tcggggtctg aggagtaggc
      361 cgcggggtcc atgttgccca gcacgacgtt ggccgtcttg cgggcgaccc cgagcagggt
      421 gaggagcccc gcgattgtgt gcggcacccg tccgtcgaag tcgcgcagca cgacctgtgc
      481 ggctcccagg atgtgtttgg tcttgttgcg gaagaacccg gtgggccgga tatccgcctc
      541 gaactcggcg gcgtcggcac cggcgtactc ttcaagcgtg cggtacttct tgaagaggtc
      601 cgccgtcacc ttgttcacca tgacgtcggt gcattgggcc gacaagatga ccgacaccag
      661 cagttcccac tcgttgccga agaccagcat gatgccggcg tcgggatact cgcggccgag
      721 ccgcgagagc acctcggccg cctgcgcggc gcgccacggc ttggggccgc gccgcacggt
      781 cggaccaccc tgcggtgcgc cgtctggagt cgatgggctc accatgccga cacctcgcgt
      841 ttcacggttc tgggccttcg tcggcaccgt cgccggagcg gcacgctggt tggttgcttg
      901 acgaacccga tcgtctcatc ggcaacggaa cgcccaaacg cgtcttcccc gggcggcaca
      961 ggcttgtctc ggtttcggcc ccgagtcgac tctgcaccac cccggtcagg agtcacgtcc
     1021 cagatcgaac gccctcatat tggcgtcgat cgtcttgggc ggcacgacct cggcgatgat
     1081 cttccgccag gtctcgtcgg caaacggcag ccggccggag atggccccca gaagcaccat
     1141 gttcgccgcc ttcgccgtgc ccgcctgttc ggcgagcgcc gtggcgtcga ctttgcgact
     1201 gttcggccag atctcggcga tgcgtgcatc gatctcgtcc gggtaggtgg ccagccccgc
     1261 cgcgactgtg ctcgggttca ccctcagcgc gttgtagatg agcagaccgc cgggacgcaa
     1321 ccagtggtgg tagcgcagtg cctccagggg ctcgaacgag aggagaaggt cggcggctcc
     1381 catggtcacg atgggcgcgg ccaccttggc gccgaagcgc aggtggctca ccacgctgcc
     1441 cccgcgctga ctcatgccgt gcacttcgct ctgcttgacg tcgtatccct cggccatcgc
     1501 ggcctgactc agcacgtagg aggccaggag gaccccctgc ccgccgacgc cggagaggag
     1561 cacgttcgtg gtctgggtgt tcacggccag ggcgggtcga gtcggcgcgg tctgggtctg
     1621 ggtattctgg gtcatggcgt ctccccctag gccttcacgg gttccggcgc caggatggcg
     1681 tcgaaattgc acatctgggc gcagaccccg caaccgttgc actggatcgg gtcgatctcg
     1741 accagcgtct tctcgccctc ctccaccagg ttgagagcga tacagcccac ccgcaggcag
     1801 cgcttgcagc cgttgcacag gttctcgtcg atcgtccagg gatcgttctt gattttgaac
     1861 tcgagcacgc aaggggcggt ggcgatgatc actgagggtt cgcctgcctc cagctcttcg
     1921 cgtatcacgc gctcggtctg cgcgaggtcg taggggtcga tgcgggctac gcgcttgacg
     1981 ccgagggcct cgacgagttt cgtaaggtcc accatggacg ccggctcccc gtcgagcgtg
     2041 ttcccgctgc ccgggttctg ctggccgccg gtcatcgcgg tggtgcggtt gtcgaggatg
     2101 atggtgaccg acgcgccgcg gttccagatg gtgtccatga gaccggtgac gccggagtgg
     2161 aagaaggtgg agtcgccgat gaccgccacc gctctgttct tgatgtcgcc ctgcgcgtcg
     2221 accaccttgg acatgccgtg ggccatgccg atgctggcgc ccatgcagac gcaggtgtgc
     2281 atcgcgccga tgggtggcaa ggcgccgagg gtgtagcagc cgatgtcacc gctcacgaac
     2341 acgcgcagct tctttaggac cgtgaagacc ccgcggtgcg agcagccggg gcagaaggtg
     2401 ggtgggcgtt gtggcagccc cgccgcctcg gccggcggcg gcaagagaag ggtcggctcg
     2461 gctcccgtgg cgccgccggc cgtgaggccg cgggcgacgg ctcccggatc cagctcgccg
     2521 cacagcgaga agaggtcctt gcccccgtcg agcttcacgt ccatgaggta gatctgctct
     2581 tcgaggaagg ggtcgagctc ctcgaccaca tagagtcgtt ccaccttcga ccggaactcc
     2641 tcgatcatct tccgaggcag ggggtacgtc atccccagct ttaggaagct ggcctgcggg
     2701 aacgcttcgc gagcgtactg gtagacggtg ccggtggcga tgatgcccac ggccgagtcg
     2761 cccatctcga tgcggttcac cggcagagtc tcggcgcgtt tcttcagatc ggtgatcctc
     2821 tgctctacct tgggatgctt gagcttcgcg aacccgggga gcatcgtata cttcggcacg
     2881 tctttctcga gtgtcgtcag cgcggggagt tccactcgct cacccagctc cacgatgctc
     2941 ttgctgtggg atatgcgggt ggtcgtgcgg aacaacaccg gcgtgtcgaa ctcctcgctg
     3001 atctcgaacg cgaccctgac caggtccttc gcttcttgac tgtccgacgg ctcgaacatc
     3061 gggatcttgg cgaagcgggc gtagttgcgg ttgtcctgtt cgttctggga gctgtgcatg
     3121 gccggatcgt cggcggtgac caagacgaag ccgcccccga ctccagtgta ggaggcggtg
     3181 aagagcgggt cggcggccac gttcacaccc acgtgcttca tgaccgccag cgagcgcgcc
     3241 cctgcgatcg acgcgcccag ggccacttca gtggccacct tctcgttcgg agaccactga
     3301 ctctcgatct ccttgtattc cgccatgttc tggaggatct cggtgctcgg ggtgccgggg
     3361 taggcggcgg ccaggcggac gcctgcttcc cacgcccccc gcgcgatggc ttcgttgcct
     3421 gaaaggagtt ccctcatctg gctgctccgg tctcttgagt gtggacgtcc ttctcgccgg
     3481 tcgcacttgg cgcgaccggg cgcatgtgac gacacggccc acgatctcgt ccagtatccc
     3541 ggtgtcgtgg gccatcacgc tggacgaaca atcatacttt ctggttggcc ggctagcaag
     3601 gcagacgaga gtgcccggag gctgtggatg agcccttggg cctcgtgcgc gggcgcacta
     3661 gggaagtccg tcgacacgtg atccggcgcc tgctctcagg ctgtacacta gggcacatga
     3721 cttcccgaga ttggcacaga acgagttccc gggcggacag cgatcttggc ctgttccgcg
     3781 tgcgtaggga cgtagccgtc tctccccgca caggcaacga gcacgagttc gtcgtgctcg
     3841 agtcggcgga ctgggtggcg gttgtggcac tcacagaaga tgatcgactg gtgttggttc
     3901 ggcagttccg gcacgggctg cgcgaggtga ccttggagtt gcccggcggg ttggtcgacg
     3961 ccgggatgag cccggaggag gccgcccggg cggagctgcg tcaggagacc ggctacggcg
     4021 gcggtgagtg ggcggaactg ggacgtctgg cccctctccc tgcggtcttc accaatcacg
     4081 tgcatgtatt cctggcgcgt ggggtgcgcc ttctgggcga tccggaaccc gatccaggag
     4141 aggatatcgt caccgatctg gtcgatgcgg aagacgcgcg ccggatggtg gagcggggcg
     4201 agatcaccca cgcccctgtc gtcgcggccc tcttcctgtg ggatctggag gcgcggagcg
     4261 gaggttcgag cgcagtgacc acgcatgtcc tcgacgcacc gactccgggc gaggagactc
     4321 caggcgagga catcggctca cttcgggatc ggatcctggc gcagctgcgt tcccacttca
     4381 ccctcaccgt ggctactgtc gggtcggtgg gcgttgagtc caggcccgtg gacgcagccg
     4441 ccgccggcgg cgccgacgaa ggcgccgagc ccggtgacgc tgacgagcct catgacgctg
     4501 acgagcccgg tgacgctgac gagcctcatg gcgctgacga gacccgagcg cctcacgccg
     4561 ccacggtctt ctacgctgtg gatcgccgcg gccgcttggt gttcttgagc aagggagaca
     4621 gccggcacgg ccggcacatc ggtgacggag gtccggtggc tgcgaccgtg gcccgtgagt
     4681 acgccgactg gcgcgagatc cagggcgtcc aggtgtgggg ccgcgccgag ccgctgcacg
     4741 gcgccgcgcg ggtgggggct ctcgccgtct acctggcccg gttcccgttc gtctccgcgc
     4801 tgctggagga cccacggctg gcggcgcgcc tgcgcggtat cgaggtgtat cggatcactc
     4861 cgcggagcgc cgccctcact gacaatcagc tcggtccatt tgggcgagaa gtgttggagg
     4921 atctgtgagg agaacgggcg gacggctgcg tggggaggtg gtctcgggac ggcaagaggc
     4981 ggcaagcttc atgacggcac gctgggttcg cgatgcgctc acacgggttc tcggcgggga
     5041 accgttcccc ggtacgctga accttcgcct ggagccgggc cctgcgctgg aatcctggcg
     5101 agcgctcaag gcctccgcgt ccggtgtgca gctcccgccc ggcgacgagg ggtcgtgcgc
     5161 gtccacactc ttcccggtga gtgtggaagg cacggtgcgg ggtgttctcc tcctgcccca
     5221 tgtggaaggg taccccgagg atatggtaga gctggttgcg cgggaatcgc tccgtgagcg
     5281 gttccggttg ggtgacgggt cggtggtggc attgtcctgg ggatcggcgg cgatgaagga
     5341 tcgccagttg gaggcgtgtc tgtccggcgc ggtcgagtcg agccgcggga acggattcgc
     5401 cgccttcgag ctcgaaggtg accttcccga ttttgacctc gccgccctcg atacgagcac
     5461 ccgggtcttc gggcgtcgcc tttctctgcc gctgttcgtg tcgagcatga ccggaggcgg
     5521 agtgggctcc ggccttatca atcgaaggct cgccgaagcg gcgcaggcga cgggcatagg
     5581 catggcagtg ggctcccaac gcttgatgct tgaggactct tcgcttgtct ccgatttccg
     5641 cgtacgtgac tgggctccgg acattcttct ctttgccgac ttggggttgg tgcacctcaa
     5701 ccgggggctc acgcgcgagc tgtgtctacg cgccgtcgag gagatcgaag ccgacgccct
     5761 catgctgtac gtcaaccctc ttcacgaggc gctccaagcg ggtggagacc tggatttcgc
     5821 cggtctgctc gaccgactgt cggatctgtg ccgcgacttt ccctacccga tcctactgaa
     5881 ggaggtgggt t
//
LOCUS       DASRMJ010000295         3810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_29931, whole genome shotgun sequence.
ACCESSION   DASRMJ010000295 DASRMJ010000000
VERSION     DASRMJ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29931"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFD74_08095"
     CDS             <1..339
                     /locus_tag="VFD74_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK49545.1"
                     /translation="LEDRLEEVEAGGRGPVGQDPQAALERLELRRAVWDALRRLSPAD
                     SEILILREFQELSYAEIAGVLDVPPGTVMSRLHAARRRMRGLLEPQISPASGGTARAT
                     GNTDGRNGHD"
     gene            332..778
                     /locus_tag="VFD74_08100"
     CDS             332..778
                     /locus_tag="VFD74_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZK49546.1"
                     /translation="MTDHDRQERISGYLDGELASEERRRLERDLAQDPELALELERMR
                     ALREVTSSMRLKGFPDQVWDSYVAGTYNRMERRIGWLFFSLGAMVLLAGGLYELAVSL
                     LRDSVDPWWIRSAVGALVIGLAVLFVSVLRDRLFILKRDPYKEVQR"
     gene            775..1089
                     /locus_tag="VFD74_08105"
     CDS             775..1089
                     /locus_tag="VFD74_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018303194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HZK49547.1"
                     /translation="MIVVTTDSIPGREVTEVLGVVRGNTIRARHLGKDILAALRNVVG
                     GEIVEYTKLMAESREQAIDRMTQRAEELGADAILAMRITTSMVMSQASEILVYGTAVR
                     LK"
     gene            1190..2536
                     /locus_tag="VFD74_08110"
     CDS             1190..2536
                     /locus_tag="VFD74_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49548.1"
                     /translation="MVGDATPSLTHRRILATWWPLAASWLLMGLEGPLISAAVARLAA
                     PKINLAAFGGVVFPIALLVESPIVMLLAASTALSRDRAAYRKIRRFMHWLAASLTLAH
                     TLLVFTPLYGFVVEGLIGAPPEVVGPARIGLAIMVPWTWAIAYRRFNQGVLIRFGHSR
                     AVGTGTVVRLASEVLALAAAFTLGSVPGIVVAALAIDAGVLSEAAYARLRVQSVLRDE
                     MSEADGRGVFGLHSFLVFYIPLSLTSIISLAVSPLGSAAMSRMPNALESLAAWPVVTG
                     VGFMFRSFGFAYNEVVVALLDEPGAAARLRRFALVMAAASSCGLLLLLVPSIGSFWFR
                     DVTGLSENLARIAQRSVWFAIPLPALSVLQSWFQGAIVHSGRTRAITEAVALSLATTA
                     VVLAVGVVWGGVIGLYVGVVALAAGDTLRTGWLALRSRAAHRNGGTDSPQPRRPAQ"
     gene            2562..3491
                     /gene="trxB"
                     /locus_tag="VFD74_08115"
     CDS             2562..3491
                     /gene="trxB"
                     /locus_tag="VFD74_08115"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZK49549.1"
                     /translation="MLDIAVIGAGPAGMTAGLYGARARARTVVFDKGLPGGQIVSTEW
                     VENYPAFPDGIAGPELGELMHKQAERFGAEFRTFSPVESIRTAGTDFVLTVDGEEVGA
                     RAVILATGAVPKKLGVPGEAEYTGRGVSWCATCDGAFFRDKVVCVVGGGDSALQEALF
                     LVKFASRVHVIHRRDELRATECIQEYCFLDDKIQFVFSRVVTEIVGEGGRVTGVELAS
                     TKGEPDILLPVDGAFIFVGVDPVNELVVEVADLDAGGFVRVDHNTLTSVPGLFAAGDI
                     SNGSLKQVVTAAAGGASAAFEALRFIEAKTRDV"
     gene            complement(3608..>3810)
                     /locus_tag="VFD74_08120"
     CDS             complement(3608..>3810)
                     /locus_tag="VFD74_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49550.1"
                     /translation="DGLGDATLSILFVDEPAVTIGQTIEAGKTQLGKVRAPAGELGAR
                     IAELTHDSGSHIHIEVMRGAPE"
BASE COUNT          549 a         1225 c         1330 g          706 t
ORIGIN      
        1 ttggaggatc gtctcgagga ggtggaggcg ggcgggcgcg ggcccgtcgg ccaggacccc
       61 caggctgcgc tcgagcgtct ggagcttcgg cgcgccgtct gggatgcgct gcgccgtctg
      121 tcgcccgcgg attcggagat cctgatcctg cgcgagttcc aggagctctc gtacgccgag
      181 atcgccggcg tgctcgatgt gccgcccggc acggtgatga gccggttgca tgccgcgcgg
      241 cggcgcatga ggggactcct cgagccacag atatcgccgg cctcgggggg caccgcccgc
      301 gccaccggca acactgacgg aaggaatggc catgactgac cacgaccgac aggagcgcat
      361 aagcgggtat ctcgacggcg aactggcgtc ggaagaacgg cgccgcctgg agcgagacct
      421 cgcgcaggac ccggagctgg cactcgaatt ggagcgcatg cgcgcactca gggaggtgac
      481 gtcgtcgatg agactgaagg gattccccga ccaggtgtgg gactcgtatg tcgccggcac
      541 gtacaatcgc atggaacgtc ggatcggttg gctgttcttc tcgctgggag ctatggtgtt
      601 gctggcgggc ggactgtacg agctggccgt ctcccttttg agggattctg tcgatccatg
      661 gtggattcgg tcggctgtcg gagcgctggt cataggcctg gccgtgctct tcgtatccgt
      721 gctgcgcgac cggctcttca tcctcaagcg tgatccctac aaggaggtcc aacgatgatc
      781 gtggtcacga ccgactccat tcccggacgt gaggtgaccg aggtgctcgg cgtcgtccgg
      841 ggcaacacca tccgtgcccg ccacctgggc aaggacatcc tggcggctct gcgcaacgtg
      901 gtcggcggcg agatagtcga gtacaccaag ctcatggccg agagccgcga gcaggccatt
      961 gaccgcatga cccagcgggc ggaggagctg ggggccgacg cgatcttggc catgaggatc
     1021 accacgtcga tggtcatgag tcaggcatcg gagatcctcg tgtacggcac ggcggtgcgc
     1081 ctgaagtagg cgcgctcggc gcgtcagttc ccggacgagg cggcgggctt cggctatcat
     1141 tggctgatgc ccgctcccac cacccgccgc cgtacgcaag cgatccacga tggtgggtga
     1201 cgccactccc tccctgacgc accgtcgtat cctcgcgacc tggtggccac tcgccgccag
     1261 ttggctgctc atgggcctgg aaggtcctct gatcagcgct gctgtcgccc gcctcgccgc
     1321 ccccaagatc aatctggccg ccttcggcgg cgtggtcttc cccatcgccc tgctggtgga
     1381 gtccccgatc gtcatgctgc tggccgcgtc caccgccctc agcagggaca gagccgcgta
     1441 ccgcaagatc cgcaggttca tgcactggtt ggcggcttcg ctcacgctgg cacacacgct
     1501 cctcgtcttc actcccctgt acgggttcgt ggtcgaggga ctcatcggcg ctccgcccga
     1561 ggtagtgggt ccggcccgca tcggtttagc gatcatggtt ccgtggacct gggctatcgc
     1621 ctatcggcgg ttcaaccaag gcgtgctcat caggttcggc cattcccgtg ccgtcggcac
     1681 cgggacggtc gtgaggctgg cctctgaggt cctggcgttg gccgcggcct tcacgcttgg
     1741 gtccgtcccc gggatcgtgg tggccgctct cgcgatcgac gcgggcgttc tctcggaagc
     1801 tgcctacgct cgcctgcggg tgcagtcagt gctcagggac gagatgtctg aggccgacgg
     1861 tcgcggggtc tttggccttc actcgttcct tgtcttctac atcccactct cgctcacttc
     1921 gatcatctct ctcgccgtca gtccgctcgg cagcgccgcc atgagccgca tgcccaacgc
     1981 gctcgagtcg ttggcggcgt ggccggtggt gacgggtgtc ggcttcatgt tccgcagctt
     2041 cggtttcgcg tacaatgagg tggtggtggc gctgctcgac gaaccgggtg cggcagctcg
     2101 gctacgccgg ttcgctctgg tcatggccgc ggcctcttcg tgtggtctgc tcctcctgct
     2161 ggtgccgtcg atcggctcgt tctggttccg ggacgtcacc ggtctgtcgg agaacctggc
     2221 gaggatcgct caacgcagcg tatggttcgc gatcccgttg cctgcactca gtgtgctcca
     2281 gagctggttc cagggagcga tcgtacatag cggtcgcacg cgcgccatca cagaagcggt
     2341 ggccctttcc ttggccacca ccgccgtggt gctggcggtt ggcgtggtgt ggggaggcgt
     2401 gatcggactc tacgtgggtg tggtcgcgct cgctgccggg gataccctgc ggaccggctg
     2461 gctcgcgctt cggagccggg ccgcgcaccg caacggaggg acggactcac cgcagccccg
     2521 aaggcccgcg cagtagacac gtggagggac ttgtgaccga gatgctggat atcgctgtga
     2581 tcggagccgg gcctgcgggc atgaccgccg gactgtacgg agcccgcgcc cgcgcccgca
     2641 cggtcgtgtt cgacaaggga ctgccgggtg ggcagatcgt cagcaccgag tgggtggaga
     2701 actaccccgc cttcccagac ggtatcgccg gtccggaact cggggaactc atgcacaagc
     2761 aggccgagcg gttcggtgcc gagttccgca cgttctcacc ggtggagagc atccgcaccg
     2821 ccgggacgga cttcgtgctc accgtcgacg gtgaagaagt gggggctcgc gcggtgatcc
     2881 ttgctactgg ggccgtcccg aagaagctcg gggtcccggg cgaggcggag tacacaggca
     2941 ggggcgtatc gtggtgcgcc acgtgcgacg gggcgttctt ccgcgacaag gtggtgtgcg
     3001 tcgtaggcgg gggcgactcg gcgcttcagg aggccctctt cctcgtcaag ttcgcctcgc
     3061 gcgtgcacgt gatccatcgg cgcgacgagc tgcgcgccac cgagtgcatc caggagtact
     3121 gcttcctcga cgacaagatc cagttcgtct ttagccgcgt ggtgaccgag atcgtgggcg
     3181 agggcggcag ggtgaccggg gtcgagctgg cttcgaccaa gggcgagccc gacattcttc
     3241 tcccagtgga cggcgcgttc atcttcgtgg gcgtggatcc cgtgaacgag ctggtggtcg
     3301 aggtcgcgga cctggacgcc ggaggcttcg tcagggtgga ccacaacact ctgacgtcgg
     3361 tgcccggcct cttcgcggca ggggacatca gcaatgggag tctgaagcag gtggtgactg
     3421 cagcggccgg cggtgcgtcc gccgccttcg aagccttgcg cttcatcgag gcgaagactc
     3481 gcgacgtctg atccgaggcg atcgcagcga aggcgatcgg aacttccctc gggctggggc
     3541 ttagacccga tgcgtcagac ccccaccctc cggacgcggc atcaggcgac gggctcaggc
     3601 cgcggcctca ctccggggcg ccgcgcatca cctcgatgtg gatatggctg ccggagtcat
     3661 gcgtcaactc cgcgatacgg gcccccagtt ccccggcggg cgcgcgcact ttgccgagct
     3721 gggtcttccc ggcctcgatc gtctggccga tggtcaccgc cggctcgtcc acgaagagta
     3781 tgctgagggt ggcgtcgccc agtccgtcgg
//
LOCUS       DASRMJ010000296         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_205714, whole genome shotgun sequence.
ACCESSION   DASRMJ010000296 DASRMJ010000000
VERSION     DASRMJ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205714"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..1463
                     /locus_tag="VFD74_08125"
     CDS             294..1463
                     /locus_tag="VFD74_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HZK49551.1"
                     /translation="MSVACARPRLPLALFLLAVAIFVCLPAASALAAPLTATTTTVVL
                     RGEVKQQVADLQGQADAVQADIDLLDLELERLTESYNRLNVDLETLNQDLNGLRRQLR
                     ETEDLCRVRQARLNKRLSDTYKNGSSGVSSLLALLMTTNDFSDFLERLFLITKVTVQD
                     QILVESISSSAGDIDRVAAQIEAKKRDALEVRRELDQKQAQIEGLLSERQSVLAGLDS
                     QIAGLVEQERQRQEAERLRLEAELRSRLAGWERYDGPLPQIADAVLGQVVETAASYLG
                     IPYIWGGDRPTTGFDCSGLLQYVYRQHGVDLPHYSGYQAEMGVPVSPADIAPGDLIAF
                     GSPVHHVGLYIGEGLFIHAPRTGDVLKITPLSTRDDVSTIRRFPLQERAGPPSID"
     gene            1475..1777
                     /locus_tag="VFD74_08130"
     CDS             1475..1777
                     /locus_tag="VFD74_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49552.1"
                     /translation="MNTVLLFVIAASLVIMAAALAYTGVTAYRLYKQVRGIQELLEPH
                     LQEFTRKQAQTTELLSSIERRQGDLSGRMQRATASASTLGYLFSEFGEARDKLRGY"
     gene            1848..>2654
                     /locus_tag="VFD74_08135"
     CDS             1848..>2654
                     /locus_tag="VFD74_08135"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZK49553.1"
                     /translation="MLTIADNLNAATPWVSAALAVRDAEAITDLALRLRDAGADALDL
                     NLGFRSRGASETAAWLVRHVWEATALPLCIDTADPHTMRAGMAAADALGAVPMPIINS
                     FSLESRKIEQILPLAAEYGAPIVGYCVRDVVPQTPEERLEVAVELVELADAAGVPRHR
                     LYLDPILFPLATFQDDFAHVLAFFEALPQLFDPPVCTMAALSNVGHGLPAAKRRVVEG
                     TVLPMLAALGIDAVLLNVLDPQLRDILTVLRAAEGRMVFSGADLAADDRGR"
BASE COUNT          436 a          962 c          838 g          418 t
ORIGIN      
        1 gctccacccc gcgccgttcg aaggagtagt ataacatacg cctcagcggc gcccacccga
       61 tccggaggcc gtctccgcgg cgtcgccggg ccatgcccgt gccgcctccg aacccgccaa
      121 tatccgacct ctggagtatc gacacgggtc aagaatcgtt gtagttttcc gatagtgccg
      181 gtgtaggacc atcggtctgt cccgcgggaa cggacccggc cgggccgcgg atacctggaa
      241 gaggaaacgt gcaccatccc cgacgatcag aggacggcag gctccgcacc cacgtgagtg
      301 tcgcgtgcgc tcgaccacgg ctgccgctcg cgctgttcct cctggccgtc gccatctttg
      361 tgtgcctccc cgccgcatcc gcactcgccg caccactgac agccaccacg accaccgtgg
      421 tcttgcgcgg cgaggtgaag cagcaggtcg ccgacctcca gggtcaggcg gacgccgtcc
      481 aggccgacat cgacctcctc gacctcgaac tcgaacgcct caccgagagc tacaaccggc
      541 tgaacgtcga tctcgagacc ctcaaccagg acttgaacgg cctgcgccgc caactgcgcg
      601 agaccgagga cctgtgccgg gtgcggcagg cccgactcaa caagcgcctc tccgacacct
      661 acaagaacgg cagctcgggc gtctccagcc tcctggccct gctgatgacc accaacgact
      721 tctccgactt cctggagcgc ctgttcctca tcaccaaggt gacggtccaa gaccagatcc
      781 tggtggagtc gatcagctcc tccgccggcg acatcgatcg ggtggcggcc cagatcgagg
      841 ccaagaagcg tgacgcgctc gaagtgcgtc gcgagttgga ccagaagcag gcacagatcg
      901 aggggctgct ctccgaacgg caatcggtcc tggcgggcct ggattcgcag atcgcgggcc
      961 tggttgagca ggaacgtcaa cgccaggagg cggagcggct gcggttggaa gccgagcttc
     1021 gctcccgctt ggccggatgg gaacgctacg acggcccctt gccccagatc gccgacgcgg
     1081 tgctcggcca agtcgtggag acggcggcct cctacctggg catcccctac atctggggcg
     1141 gcgacagacc caccaccggg ttcgactgct ccggactgct ccagtacgtc taccgccagc
     1201 acggtgtcga tcttccgcac tactccggct accaggccga gatgggcgtc ccggtgtccc
     1261 cggcggatat cgctcccggc gacctcatcg ccttcggttc cccggtgcat cacgtgggcc
     1321 tctacatcgg tgagggactc ttcatccacg ccccccgcac gggcgacgtt ctgaagatca
     1381 cccccctctc cacgcgcgac gatgtcagca ccatccgccg cttcccgttg caggagaggg
     1441 caggaccgcc ctcaatcgac tagaatagag cactatgaac actgtgctcc tcttcgtcat
     1501 cgcggcatcg ctggtcatca tggcggcagc cctggcctac acgggcgtca ccgcctaccg
     1561 actctacaag caggttcgcg ggatccagga gctgttggag ccgcacctcc aggagttcac
     1621 ccggaagcag gcgcagacga cggagctgct gtcgagcatc gagcgccgtc agggtgatct
     1681 gagcgggcgt atgcagcgcg ccaccgcctc ggcctcgacc ctgggctatc tgttctcgga
     1741 gttcggcgaa gcccgagaca agctgcgagg ctactgatcg gccgcggcga ccgccgcact
     1801 cacggcctga gccgcggcaa cggtcgcggc cgtagccgca ccatctgatg ctgaccatag
     1861 ccgacaacct caacgccgcc actccctggg tgtccgccgc tctggccgtc cgtgacgccg
     1921 aggccatcac cgacctggcc ctccgcctgc gcgacgcggg cgccgacgcc ctcgacctca
     1981 acctcgggtt caggagcagg ggtgcgtccg agacggccgc ctggttggtg cgccacgtgt
     2041 gggaggcgac ggcactcccc ctgtgcatcg acaccgccga cccgcacacc atgcgcgcgg
     2101 gcatggctgc ggcggacgct ctgggagcgg tccccatgcc gatcatcaac tccttctcgc
     2161 tggagagccg gaagatcgag cagatcctgc ccctggcggc ggagtacggc gcccccatcg
     2221 tgggctactg cgtccgtgac gtggtgccgc agacgccgga ggagcggctg gaggtggcgg
     2281 tggaactggt ggagctggcc gacgcggccg gggtgccacg gcaccgactc tacctcgacc
     2341 ccatcctctt ccccttggcc acgttccagg acgacttcgc gcacgtgctg gccttcttcg
     2401 aggcgctgcc ccagctcttc gacccgccgg tgtgcacgat ggccgccctc tccaacgtgg
     2461 gccacggcct cccggcggcg aagaggcgag tcgtggaggg gacggtgctc cccatgttgg
     2521 ctgccctcgg gatcgacgcc gtgctgctga acgtgctgga cccccagctc agggacatcc
     2581 tgaccgtgct gcgggcagcc gaaggccgca tggtgttctc gggagccgac ctggccgccg
     2641 acgaccgggg ccga
//
LOCUS       DASRMJ010000297         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_63351, whole genome shotgun sequence.
ACCESSION   DASRMJ010000297 DASRMJ010000000
VERSION     DASRMJ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63351"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..2317
                     /locus_tag="VFD74_08140"
     CDS             83..2317
                     /locus_tag="VFD74_08140"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HZK49554.1"
                     /translation="MSDERAVDVAADETVPARAASDPATDSATGSAAASAADSAAKAV
                     VAKPVGSAADSVAGDPRVGVFVCDCGTNIAATVDCPSVTEYAAGLGDVVFAEEGKWIC
                     SVDYLTRIQEAVKANNLNRAVVACCTPRTHEKLFKNTVGAAGMNPYQLEFVSIREQCS
                     WVHKTDKAVATQKAKDLVKMGVAKARLLEEGEEIRLPVKTDCLVIGGGVAGMTAALAV
                     AAQGFVVRLVEKQDGLGGLLKGVNTVGPEGVSADEVVTRLVERVTANENVEVYTNTRL
                     TAIGGYIGNYKVEVDRAGVPGAFDSSTIIVATGMRQLDATGHYGYGTGDDVITQLELE
                     RRLKAGGALGAKNVVIVQCVRSRNEERGCCHIGCTVALKNALEIKKRDPEANVYLLYK
                     DLITVKDEYAHMKEVLREGVTTVRFSDARKPEVTRDAGGHAVVKVYDVLLGQEITLPA
                     DLVVLTVGFEGDESVTDLRGLLKVSASSDGFFTEAHIKLGPLDFAAAGVYLAGSARSP
                     KPLRDVSEEALGAAMRASIPMAKGFVEAEGIVATVDLANCSQCGLCWKNCPFGAIEEV
                     DKNPHILEALCKGCGLCAADCPKECIQIVHYGDDQIAAQIAAALEDNPEEKIIAFVCH
                     WCALGAVDIAGVGRSEYPANARIVRVMCSARVAQKYVIDCYDRGAAGVLVAGCEFPTC
                     HYITGNYKCSDRLVRLRKKMERKGYDTSKLWEVWLSASMGPKWVATMKEMTEALGLAP
                     ACKS"
     gene            2325..>3515
                     /locus_tag="VFD74_08145"
     CDS             2325..>3515
                     /locus_tag="VFD74_08145"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK49555.1"
                     /translation="MTTNVNIDITSRGSGAHAGATDRAVASRASNAAAAKEPIISCEQ
                     CGMCSSACPITGVEDFNIRRIERAVQLGVVDGLLESGLPWLCTTCGRCEAACPNGYRI
                     MTTTRTLRTMVAEELQPSMAPCRKACPAGMDAPRYIRMIAEGRFADAYAVIRDSAPLP
                     GVLGRVCAHPCEDQCRRKAVNQPISICSLKRFAYDQAAADFPVVGTAGDSGKRVAVVG
                     SGPAGLTAAFHLRKRGHAVTLLEAREEAGGMLRYGIPAYRLPQEVLDKEIGDILAMGV
                     DFRPGTTLGEQVTLDGLHAEYDAVLVAVGAQQSRKVKLEGADHPDVLWGGEFLADVRA
                     GVDVKLKDRVVVVGGGNVAVDVALTALRSRAATVTMVSLESREEMPAFDWEVEEALQE
                     GVELV"
BASE COUNT          598 a         1138 c         1235 g          544 t
ORIGIN      
        1 tctcggagag cgtggtgcag gcgacggccg cgagcatgaa agcggtgcag cgccgcgtgc
       61 gcgtggccga ggaggccgcc gtatgagcga cgagcgcgcc gtagacgtgg ccgcggacga
      121 gaccgtgccg gcccgcgccg catccgatcc ggcgactgac tcggcgactg gctcggccgc
      181 agcgtcggcg gccgactccg ccgcgaaggc cgtcgtcgcc aaaccggtcg gttccgcggc
      241 cgactcggtg gccggcgatc ctcgggtggg cgtcttcgtc tgcgactgcg gcaccaacat
      301 cgccgccacc gtggattgtc ccagcgtcac cgagtacgcc gcgggcctcg gcgacgtggt
      361 cttcgccgaa gaaggtaagt ggatctgttc ggtggattac ctcacccgca tccaggaagc
      421 ggtcaaggcg aacaacctga accgggccgt cgtggcctgt tgcacgccgc gcacccacga
      481 gaagctcttc aagaacaccg tgggcgctgc gggcatgaac ccctatcaac tcgagttcgt
      541 gagcatccgc gagcaatgct cctgggtgca caagaccgac aaggccgtgg ctacccagaa
      601 ggccaaggac ctggtcaaga tgggcgtggc aaaggctcgc ctgctcgaag agggcgagga
      661 gatacgtctc ccggtgaaga ccgactgttt ggtgatcggt gggggcgtgg cgggcatgac
      721 cgccgccctg gccgtggccg cccagggctt cgtggtgcgg ttggtggaga agcaggatgg
      781 gctgggcggc ctgctgaagg gagtcaacac cgtcggtcct gagggcgtgt cggcggatga
      841 ggtggtcact agactcgtcg agcgggtgac cgcgaacgag aacgtcgagg tgtacacgaa
      901 cacccgcctc accgcgatcg gcggatacat aggcaactac aaggtggagg tggaccgcgc
      961 cggcgtcccc ggtgccttcg actcctccac catcatcgtg gccaccggca tgcgccaact
     1021 cgacgctacc ggccactacg gctacggcac cggcgacgac gtcatcacgc aactggagtt
     1081 ggagaggcga cttaaggcgg gcggcgccct cggcgccaag aacgtggtca tcgtgcagtg
     1141 cgtgcgctcg cgcaacgagg agcggggctg ttgccacatc ggctgcaccg tcgccctcaa
     1201 gaacgcgctc gagatcaaga agcgcgatcc cgaggccaac gtctacctgc tctacaagga
     1261 cttgatcacg gtcaaggacg agtacgccca catgaaagaa gtgctgcgtg agggcgttac
     1321 caccgtacgt ttcagcgatg cccgcaaacc cgaagtgacg cgcgacgccg gcggccacgc
     1381 agtagtcaag gtgtacgacg tgcttctcgg acaggagatc acgctgcccg ccgacctcgt
     1441 cgtgctcacg gtgggcttcg aaggcgacga gagcgtcacc gacctgcggg gtctgctcaa
     1501 ggtctccgcc agctccgacg gcttcttcac cgaggcgcac atcaagctcg gccccctgga
     1561 cttcgccgcg gccggcgtct acctggccgg atcggcccgc tcgcccaagc ccctgcgcga
     1621 cgtcagcgag gaggctctgg gcgcggccat gcgggccagc atccctatgg ccaagggctt
     1681 cgtcgaggcc gagggcatcg tggccacggt cgatctggcg aactgctcgc agtgcggtct
     1741 gtgctggaag aactgcccct tcggcgccat cgaagaggtg gacaagaacc cccatatcct
     1801 cgaagccctc tgcaagggct gcggcctgtg cgccgcggac tgccccaagg agtgcatcca
     1861 gatcgtccac tacggcgacg atcagatcgc cgctcagatc gcggcggcgc tcgaagacaa
     1921 tccggaggag aagatcatcg ccttcgtgtg tcactggtgc gcattgggtg cggtggacat
     1981 cgccggcgtg gggcgctccg agtatccggc caacgcccgc atcgtgcggg tgatgtgctc
     2041 ggcgcgcgtg gcccagaagt acgtgatcga ctgctacgac cggggcgccg cgggggtgct
     2101 ggtggcgggg tgcgagttcc ccacctgtca ctacatcacc ggcaactaca agtgctccga
     2161 ccggctggtg cggctgcgca agaagatgga gcggaagggc tacgacacga gcaagctgtg
     2221 ggaggtgtgg ctctcggcgt cgatggggcc gaagtgggtg gccaccatga aagagatgac
     2281 cgaagccctg ggcctcgccc ccgcgtgcaa gagttgagga tgtcatgacc acgaacgtca
     2341 acatcgacat caccagccgc ggctcgggcg cccatgccgg cgccaccgac cgcgccgtcg
     2401 cgtctcgcgc gtcgaacgcc gccgcagcca aggagcccat catctcctgc gagcagtgtg
     2461 gcatgtgcag ctccgcgtgc cccatcaccg gggtcgagga cttcaacatc cgccgcatcg
     2521 agcgggccgt gcaactcggc gtggtcgatg gtctgttgga gtcgggcctg ccgtggctct
     2581 gcaccacctg cgggcgctgc gaagctgcct gtccgaacgg ttacaggatc atgaccacca
     2641 cgcgcaccct gcgcaccatg gtagcggagg agcttcagcc gtccatggct ccctgccgca
     2701 aggcctgccc ggccggcatg gatgcgccgc gctatatccg aatgatcgcg gagggccggt
     2761 tcgccgacgc ctacgcggtc attcgcgatt cggcgcctct gcccggagtg cttggccgcg
     2821 tgtgtgcgca tccgtgtgaa gaccagtgcc ggcgcaaggc cgtgaaccaa ccgatctcga
     2881 tctgctcgct caagcgcttc gcctatgatc aggcggccgc cgacttcccg gtcgtcggca
     2941 cggctggcga cagcggcaag cgggtggcgg tggtggggtc cggcccggcc ggcctcaccg
     3001 cggccttcca cctgcgcaag cggggccacg ccgtcaccct gctggaggcc cgcgaggaag
     3061 cgggcgggat gctccgctac ggcatccccg cctaccgtct gccgcaggag gtgctcgaca
     3121 aggagatcgg cgacatcttg gcgatgggcg tggatttccg tccgggcacg acgctgggtg
     3181 agcaggtgac cctcgatgga ctccacgccg agtacgacgc ggtgctcgtg gccgtaggcg
     3241 cccaacagag ccgcaaggtg aagctcgagg gtgcggatca tcccgatgtc ctatggggag
     3301 gggagttcct ggccgatgtg cgggccggcg tggacgtgaa gctcaaagac cgggtggtcg
     3361 tggtgggcgg aggcaacgtg gcggtcgatg tggccctcac ggccctgcgc tcccgtgccg
     3421 ccaccgtgac catggtgtcg ctggagagcc gcgaggagat gcccgccttc gactgggagg
     3481 tggaggaggc gctccaggag ggcgtggaac tcgtg
//
LOCUS       DASRMJ010000298        13416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_64360, whole genome shotgun sequence.
ACCESSION   DASRMJ010000298 DASRMJ010000000
VERSION     DASRMJ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 13416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13416
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64360"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFD74_08150"
     CDS             complement(<1..848)
                     /locus_tag="VFD74_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49556.1"
                     /translation="MRLSSIAFRNVRRHLSRSALMIIAISITAAVVMTLFLVGRSAER
                     DLADKVDQYGANIVVVPRSKDLPLTYGGVQLGGITYDVQPLHSEDETKIRSIKNKENI
                     ARVAPKLVERGEVGGVPVLVAGVRWVDELALKRWWHITGAAPSDSADALLGARAAERL
                     GLTAGSDMTVRGETLRVTGVLASTGTQEDELVYIELATAQRLWNRPGEFSFIEVAAWC
                     STCPIETINAQISAEIPNARVSAVLKAAESREILIGQFRLFSWVLSGFMTLVGCLIVI
                     TTTLGSV"
     gene            complement(922..1608)
                     /locus_tag="VFD74_08155"
     CDS             complement(922..1608)
                     /locus_tag="VFD74_08155"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HZK49557.1"
                     /translation="MPDTDHVVDNLEVKRQRFEEAGRFPVRKVVIIGVIVAVAAVASF
                     VGYQQWQVSRAVGGVIVAPGIKYPAYEVDMVTLDNATQTPDGISFPLADLKKDLLVGL
                     VYQRSNPMPEGYQLAAGGNVLPVMAYISPSGRLVVATSFCEPCRSTLFHFEGNQLVCD
                     VCFTRWDLNTLLGIGGGCFDYPPEELAAEVKGDMVFVPKADLEAWVPRAYQEVATSSD
                     TTMSTLPPAQ"
     gene            complement(1773..2708)
                     /locus_tag="VFD74_08160"
     CDS             complement(1773..2708)
                     /locus_tag="VFD74_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZK49558.1"
                     /translation="MAAAAATMLLLGALLLRYLPHGILWKAGLTCAMEDGCPPPLPLW
                     AGAGFSFLIAGVAVGLVSFWALAVGSQLSCSGRRCHDVLREARSFGGRVPAGLRNRVL
                     LVADDALLSYTIGFISPWVVISTGLRDALDDDELFAVLAHEEGHVLRRDNLVILVGQT
                     IALTFALIPGVRACFASLRRCQELAADLFARERTGDGLVVASSLHKFARSVLAPSLRQ
                     APPVQAGVGFADEGNVGERIRGLLVDELVVTSKRRLVVAMAALAIMLVGFTGSAAAFT
                     RVTLASGADCAACHHSALGHPVPTAGHGVCSPEIV"
     gene            complement(2813..3250)
                     /locus_tag="VFD74_08165"
     CDS             complement(2813..3250)
                     /locus_tag="VFD74_08165"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZK49559.1"
                     /translation="MDHTINLNTGNQGLRQVLGELEAEIMECMWEFGSASVREVHECL
                     AEKREIAYTTVMTVMSRLAEKGMLRRSQEGRAYVYVPVETRDAFCTSVVKRVLDGLFA
                     GAGKPVLAHFVENLTEADSAELDGLAQVIEEKRRERSSSAPQQ"
     gene            complement(3327..3470)
                     /locus_tag="VFD74_08170"
     CDS             complement(3327..3470)
                     /locus_tag="VFD74_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HZK49560.1"
                     /translation="MNQIGFLFAGYAVIWVVLGTYFVFVGRRQTALKREVERLERELQ
                     AQA"
     gene            complement(3498..4316)
                     /gene="ccsA"
                     /locus_tag="VFD74_08175"
     CDS             complement(3498..4316)
                     /gene="ccsA"
                     /locus_tag="VFD74_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZK49561.1"
                     /translation="MTGTISDQEQGPDARAEDGGPPLTSGSGGSSAVPGSGGAPAVSG
                     PGGRKLWIDLLGLGSVALILMALYGALIVAPTEAVMGDVQRIFYFHMPSAWVALGPAF
                     TVVFVCSIIYLAKRDLKFDRIAGASAEIGVMFTTITLVTGPLWARPIWGAYWTWDPRL
                     TTTLVLWFIYVAYLMLRSSTAEGHKRARLAAVFGIVGWIDVPIVFMSIRWWRTIHPTL
                     INGQGMQMDPTMQAVLMFSLFAFTILYVYLLIVRVRQLDLGSRVDRLRQQLLMR"
     gene            complement(4319..4996)
                     /locus_tag="VFD74_08180"
     CDS             complement(4319..4996)
                     /locus_tag="VFD74_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZK49562.1"
                     /translation="MGFLSRSWAIAQKDIIAELRARETVFSMLLFGFLVILIFNFGIE
                     PGLREENLVKPGVLWAAFIFAGILGLNRSFASEKENDCLQGLLLCPVDRAVIYLGKLL
                     GNVVFTFIMGIVVFVFFSVLYNVDIIATFLPIGLILLLTTIGFSAVGTLFSAVSVGIK
                     ARDVILSILLFPVMVPVVLAAAKSSASVLDGGGLSGGGLWLVLLVVYDVIFLAVSVMV
                     FDYAVEE"
     gene            complement(4984..5721)
                     /locus_tag="VFD74_08185"
     CDS             complement(4984..5721)
                     /locus_tag="VFD74_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49563.1"
                     /translation="MTHAPEPAIEVRGLWKYFGHFPALRGLDLTLAKGRFLTIFGPNG
                     AGKTTLIRVLSTQLKPSEGSVSVGGVDVAARADLLRHRIGLISHNTYLYAHLTALENL
                     LFFGRMYGLADPRTRAMDLLTEVGLAGRMDDRVSGYSRGMQQRLSIARALLHDPDIMF
                     LDEPYTGLDQHASRMLRGVLETLHTRDRTIVLTTHNIDQGLEMCDDVAIQVRGRLVFK
                     EPIGAIDRGTFEEAYFEHVGKDYRWDS"
     gene            complement(5757..6179)
                     /locus_tag="VFD74_08190"
     CDS             complement(5757..6179)
                     /locus_tag="VFD74_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK49564.1"
                     /translation="MIGTLFGVVLALLAVGFIAYPLLREQLGDDETFDLPEDLEVLLR
                     RKESTYSALKELEFDFKTGKLSEGDFHELDARYRADALEILAAIDARDRGASGAAGSR
                     KGKVGGQKAGAAPACAECGETLKPGQRFCASCGAEVQD"
     gene            complement(6238..6801)
                     /locus_tag="VFD74_08195"
     CDS             complement(6238..6801)
                     /locus_tag="VFD74_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HZK49565.1"
                     /translation="MRSEFPVRVAATPDSVDKGSRGRRRRAPLRFIVLMAVAVAVLVP
                     SVAFAATPPRSSVSDKIICQCGCGAVLTNCPHQNCGWGVPAKAFIDEQLVKGKTSDEL
                     VQYYVSQYGEVVLAAPTKTGFNVIGWVMPFVGLIVGAVAIYFLIGMWSARRDEETVPA
                     EGATALELPDPNDPIARRLNDELRDFD"
     gene            complement(6791..8791)
                     /locus_tag="VFD74_08200"
     CDS             complement(6791..8791)
                     /locus_tag="VFD74_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HZK49566.1"
                     /translation="MNQVGYTALLLGLMIAAYGVVAPLIGVRTGRREWIKSAENATYA
                     FAGVAIVASAALFYALITRDFSNEYVASYTSRELSWFYTVSAFWAGNSGSMLLWVLLL
                     GVFAAIAVYQNRHKNREILPYVISVLMVVGLFFTILLTTSAGSNPFAVIPGGGPANGE
                     GLNPMLENPGMVIHPVTLYLGYVGFSIPFAFAMGALITKRLGDFWIRSTRRWTIIAWI
                     FLTIGNIVGAWWAYVTLGWGGYWAWDPVENASFMPWLTGTAFLHSVMIQEKKDMLKVW
                     NIILIILTFCLTIFGTFLTRSGVISSVHSFGQSSLGPFFIGFLGLILVFSLNLLFSRL
                     DLLKSRNELDSFVSRESSFLLNNLILVGMAFTVLWGVLFPIISEAVTGNKVTVGPPFF
                     DQVMAPIGLVLMFVTGICPLIAWRRATLANLGKNFMYPAAVGAIAAVLLVALGMRHVY
                     AIISFSLSAFVLSAILVEFGRGAWVRREMSGENIVRALGSLVWRNKRRYGGYTVHVGV
                     ILLLVGVTGSYAFKQEASQNLNKGDTISVGAYSLTYDSMETYSTDEKQVGRATFSVKK
                     DGKDVGVVYPIREYYFAKDQPWTRIDRISTLSRDVYVMMLDYSAETGEVNMQVDINPL
                     VSWLWIGGIVMVLGGLLAIWPDRRDSLRLAARYERQARLHEI"
     gene            complement(8829..9269)
                     /locus_tag="VFD74_08205"
     CDS             complement(8829..9269)
                     /locus_tag="VFD74_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZK49567.1"
                     /translation="MGSKKLKFLIGGLVVVGAIASLAFFAIRENMVYYYTVTEALANG
                     EAVNVRVAGDLLNGTLEKGGIGDPIRFEIYDKAVPESTLYVEFTGVVPDSFKDDPATP
                     IEVVVEGDLLKDGTFAADFMMAKCPTKYERTGADATGETSTSNN"
     gene            complement(9337..9798)
                     /locus_tag="VFD74_08210"
     CDS             complement(9337..9798)
                     /locus_tag="VFD74_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZK49568.1"
                     /translation="MSAVVAPRGPRKLTSRTLKVAAAIIAGVAAAVWLALFVSVGGDF
                     YRTVEEANAGGAAADIRVGGRVAADSVVQEGGAARFVLEGESGETLEVRYTGPFPERL
                     RAHEEAVVRGSMESGRGLEATEVLIKCPDGLFPEKITNTVLSGVGLKRLLY"
     gene            complement(9850..10482)
                     /locus_tag="VFD74_08215"
     CDS             complement(9850..10482)
                     /locus_tag="VFD74_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49569.1"
                     /translation="MRPRHLITLVFLAVLLAAGVWSFYGNVLNPHGDQTMPGMDMAAS
                     DTPADAPGDSGAGGATSAEADLLPSRLGEWGLSTSVTGEAALVQVEQLHGKSLGDGIT
                     AAWVGQYGGEGGGQPHATIWVSRCPSIDSAQELFRRMTDRISAGGSPFTGLRPIEGQA
                     VEGYALDGMGQTHYYFLIEKDLYWLAVDPEQAEASLGALISGASGKVSNG"
     gene            complement(10522..11583)
                     /locus_tag="VFD74_08220"
     CDS             complement(10522..11583)
                     /locus_tag="VFD74_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK49570.1"
                     /translation="MSSRDMENQGRSRVAALGMREGRLGLRTVLLMSLLAVALVIAWG
                     ATATPAQAAEITATQMKVSVWPEYDDPRVLVIYQADLDPSVQLPVDVTYNIPKGAEIG
                     MACEVDSGGGHACKPYTLVDKGDYQALTYKVEAQHKIFFEYYYDAFPAGTEARAFDFV
                     LRPGLAAANTTIEVQQPLRSTGFTLDPALSQVTTDSEGLKYYSQDYPDLPVGEPFTVK
                     VAYNKVDNDPSVQKDDNASAQPGTTSAAGGGGNNRALFIVLAALAFATLVFGGYKAFR
                     PAAATSNGRNRSSGARSTRAHVSRDQRASGGPVAPRRKADTGGGASDADAKSKFCVSC
                     GTQLRRQDRFCPECGDDQA"
     gene            complement(11750..12892)
                     /locus_tag="VFD74_08225"
     CDS             complement(11750..12892)
                     /locus_tag="VFD74_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK49571.1"
                     /translation="MYDSATSKPRLRAWPHTLGKFFVVTTAMAMALLLFGGLAHAATT
                     TTDAPADATASTVTTSAADAPVSGLIPAPEGFEPPNPGLLAAQMGVQFWPEYDTGDVL
                     VLLDVTLPATTVFPYTLTFFVPRGARLAGIAEIDETGAFDYTLGTPVIVPGEVMDAVT
                     VTVPKRPVLRFEYYYDPGVGGPGAKSFPVQFQAPADVGELYVEVQQPLAASDFSAGAV
                     LDQSTTDAEGFTFHYGSVSGVKAGDIFQTQVDYTKTSADPSVSSAAPESDPGQQRSSS
                     YLLVLLVVLVVAIGGIIVYRLFIRKQPIAGAGRLPSQRPASRSGRGTDRTSTKGGPTS
                     SRSTTRQARNDSAENVGGPARFCTECGGRLSKKDRFCSECGAARDS"
     gene            complement(12916..>13416)
                     /locus_tag="VFD74_08230"
     CDS             complement(12916..>13416)
                     /locus_tag="VFD74_08230"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZK49572.1"
                     /translation="DDYGVPTDLPWAMSFPKGSPPTTQLVHPTQIYESLMSLAILAVL
                     LLLLRPRLVRAGSLFWAYLALAGVERFVVEFARTNQPVALGLTQAQWISVALFAGGIA
                     IAWWLESHRATGFPAGGVTSAGARSGAGAGPGHRGAGRSSQSGKSPRTPKKHSSRRRR
                     DRRPRR"
BASE COUNT         2552 a         4402 c         4297 g         2165 t
ORIGIN      
        1 acgctgccga gcgtggtggt gatgacgatg aggcacccga ccagcgtcat gaacccggaa
       61 aggacccagc tgaagaggcg gaactgtccg atgaggatct cacgcgattc ggccgccttc
      121 agcacggcgg agacgcgcgc attcgggatc tcggcagata tctgtgcgtt gatggtctcg
      181 atcgggcagg tgctgcacca cgccgccacc tcgatgaagc tgaactcacc aggcctgttc
      241 cacagccgct gggcggtggc cagttcgatg tagaccagct cgtcctcttg tgtgccggtg
      301 gaagcgagga cgcccgtcac ccgaagggtc tcgccgcgga cggtcatgtc ggagcccgcc
      361 gttagaccga gccgttccgc cgcgcgggca ccaagcagtg cgtccgcgga gtccgacggc
      421 gcggcgcccg tgatgtgcca ccagcgcttc agggccagct cgtccaccca gcggacgccc
      481 gccaccagca cgggcactcc ccccacctcg ccgcgctcca cgagcttcgg ggccacccgg
      541 gcgatgttct ccttgttctt gatggagcgg atcttcgtct cgtcctcgga gtgcaggggt
      601 tggacgtcgt aggtgatgcc gcccaattgg acccccccgt aggtgagagg cagatccttg
      661 cttcggggca cgacgacgat gttggccccg tactgatcca ccttgtcggc caggtcgcgc
      721 tcggcgcttc tgcccaccag gaacagggtc atgaccacgg ccgccgtgat ggatatggcg
      781 atgatcatca gggccgaacg gctgaggtgc cgccggacgt tgcggaacgc tatgctggag
      841 aggcgcatgc agtcagagcc gaggtccagg agcctctcaa ggcgacggcc gcgcgcgacg
      901 gctgagtccc gcgggccacg ttcactgggc ggggggaagt gtgctcatgg tggtgtcgct
      961 ggatgtcgcc acctcctgat acgccctcgg gacccaagcc tccagatccg ctttcggcac
     1021 gaataccatg tcgcccttga cctcggccgc gagttcttcg ggcgggtagt cgaagcaacc
     1081 acccccgatg cccagtaggg tgttcagatc ccaccgggtg aagcagacgt cacagacgag
     1141 ctggttgccc tcgaagtgga acagggtgga gcggcatggt tcacagaagg acgtggcaac
     1201 gaccaagcgc ccgctcggcg agatgtacgc catcacgggc aggacgttcc cgcccgcagc
     1261 caactgatag ccctcgggca tcgggttgct gcgctggtac accagaccga cgaggaggtc
     1321 cttcttgagg tccgccagcg ggaacgagat gccgtcggga gtctgcgtcg cgttgtccag
     1381 ggtcaccatg tcgacctcat aggcgggata cttgataccg ggagccacga tgacccctcc
     1441 caccgcacga gagacctgcc actgctgata gcccacgaac gaggccacgg ctgccaccgc
     1501 gacgatcacc ccgatgatca cgaccttgcg cacggggaag cgccccgcct cttcgaagcg
     1561 ttgccgcttg acctccagat tgtcgactac gtggtcggtg tcgggcaccg cgaccccctc
     1621 acttcatcga agacgtgctt tcgctcacac gcatgcggca ttgtactaca cagcgtagta
     1681 tgccgccaag aagccgaact gtgggaagga tccgcaaaga ccgggagtcg cctcggatgg
     1741 aggatgtgtc tgggacgacg cgtgcggggc gatcagacga tctcgggcga gcagaccccg
     1801 tgacctgcgg tcggtaccgg atggcccagg gcactgtggt gacaagcggc gcagtccgcc
     1861 cctgaggcca acgttacccg agtgaacgcc gcggcgctcc cggtgaagcc gaccagcatg
     1921 atggcaagag cggccattgc caccaccagc cgccgcttcg aggtgaccac caactcgtct
     1981 accaggagac cacggatgcg ctcgcctacg ttgccctcat ctgcgaagcc cacccccgcc
     2041 tgaaccggcg gagcctgccg aagggagggc gccaagaccg accgagcgaa cttgtggaga
     2101 ctcgacgcca cgacgaggcc gtcgccggtg cgctcgcgcg cgaaaagatc cgcggcgagt
     2161 tcctggcacc gacgcaggct cgcgaaacac gcccgcacac ccggtatcag ggcgaaggtc
     2221 agggcgatgg tctgacccac gaggatgacg aggttgtctc gccgcagcac gtgcccttcc
     2281 tcgtgcgcaa gcacggcgaa cagttcatcg tcatcgagcg cgtcgcggag cccggtggag
     2341 atgaccaccc acggactgat gaagccgata gtgtaggaga gcaaggcgtc atcggccacg
     2401 agcaggaccc tgttccggag gcccgccggc acccgcccgc caaaagaccg agcctcacgc
     2461 agaacgtcgt ggcaacggcg gccggagcaa ctcaactgcg agcccacggc caacgcccaa
     2521 aacgagacca atcccaccgc gaccccggcg atcagaaacg agaatcccgc cccggcccac
     2581 agaggcaagg gcggggggca accgtcttcc atggcacagg tgagaccggc cttccacagg
     2641 ataccgtggg gaaggtaccg caatagcagc gcgcccaaca ggagcatcgt ggccgcggcg
     2701 gctgccacga tagacgccag gtaggaccac atgagggtgc gcgggtgggc acgcccttcg
     2761 agcagtcgcc gcaccagcca ggggacggcc aggatcgccg agtagctgag gactactgct
     2821 gcggtgccga tgacgaccgc tcccggcgct tctcctcgat cacttgggcc aggccgtcga
     2881 gctcggcgct gtcggcctcg gtgaggttct ccacaaaatg ggcgaggacg ggcttgcccg
     2941 caccggcgaa gagcccgtcc agcacgcgct tgaccacact ggtgcagaaa gcatcacggg
     3001 tctcgacggg aacgtagacg taggctcgac cttcctggga acgccgcagc atgcccttct
     3061 cggcgaggcg actcatcacc gtcatcaccg tggtgtaggc gatctcgcgc ttctccgcga
     3121 gacactcgtg gacctcacgc acagaggcgg agccgaactc ccacatgcac tccatgatct
     3181 cggcctcgag ctcgcccaga acctgccgta agccctggtt ccctgtgttc agattgatgg
     3241 tgtggtccat gggcttcctc ctccctccga ctcggaccca atactatcaa gcgtagtagg
     3301 tgagccgcaa ggcgggggcg tgcgggtcaa gcctgagcct gcaactcgcg ctcgaggcgt
     3361 tcgacttcgc gcttgagcgc ggtctgtctc cgccccacga acacgaagta ggtgcccagg
     3421 accacccaga tgaccgcgta gccggcgaac aagaaaccta tctgattcat gcctccacct
     3481 tcttgggatc caggggacta tcgcatcaag agctgctgcc gcagacgatc cacgcgcgag
     3541 cccagatcga gttggcgcac gcggacgatc aacaggtata cgtacagtat cgtgaacgcg
     3601 aacagcgaga acatgaggac cgcctgcatc gtcgggtcca tctgcatgcc ctgaccgttg
     3661 atgagcgtgg gatggatggt ccgccaccac cgtatcgaca tgaacacgat gggaacatcg
     3721 atccaaccga cgatcccgaa gaccgccgcc aatcgggccc tcttgtggcc ttccgccgtg
     3781 gacgacctca gcatgagata cgcgacgtag atgaaccaga gcacgagggt ggtcgtgagg
     3841 cgcgggtccc aggtccagta ggctccccag atggggcggg cccacaacgg cccggtgacc
     3901 aacgtgatcg tggtgaacat tacgccgatc tcggccgaag cgccggcgat gcggtcgaac
     3961 ttgagatcgc gcttggccaa gtagatgatg ctgcacacga agaccacggt gaaggcgggg
     4021 cccagcgcca cccacgcgct cggcatgtgg aagtagaaga tcctttggac gtcgcccatc
     4081 accgcttcgg tgggggccac gatcagcgcg ccgtagagcg ccatcaggat gagggcgacg
     4141 gagccgaggc ccagcaggtc tatccataac ttgcggccac cggggcccga aaccgccggc
     4201 gcgccaccgg agcccggaac cgcagacgag ccgccggagc ccgaggtcaa cggagggccg
     4261 ccatcctccg ctcgggcgtc cggcccctgt tcttggtccg agatagtccc ggtcatcgct
     4321 attcctccac cgcgtagtcg aacaccatga cggagaccgc caggaagatc acatcgtata
     4381 ccacgagcaa caccagccac aggccgccac cgctcaggcc gccgccgtcc aacaccgagg
     4441 ccgacgactt ggccgccgca agcacgacgg gcaccatcac cgggaacagg aggatggaga
     4501 ggatcacgtc ccgggccttg atgcccaccg acaccgcgga gaagagcgtg cctaccgcgc
     4561 tgaatccgat ggtcgtcaga agaaggatca ggccgatggg aagaaaggtc gcgatgatgt
     4621 ccacgttgta gaggacggag aagaatacga acaccacgat gcccatgatg aacgtgaaca
     4681 cgacgttccc cagaagcttg cccaggtaga tcacggcccg atcgacgggg cacagcagga
     4741 gcccctgcag acaatcgttc tccttctccg acgcgaacga gcggtttagc cccaagatgc
     4801 ccgcgaagat gaaggccgcc cacaacaccc ccggcttcac caggttctct tcgcgcagac
     4861 ccggctcgat gccgaagttg aagatcagga tgaccaagaa gccgaacaga agcatggaga
     4921 agaccgtctc gcgggcccgc aactcggcga tgatgtcctt ctgggcgatg gcccacgagc
     4981 ggctcaggaa tcccatcggt agtccttgcc cacgtgctcg aagtaggcct cttcaaaggt
     5041 gccgcgatcg atcgcgccga tcggctcctt gaagaccagg cgaccccgaa cctggatggc
     5101 gacgtcgtcg cacatctcga ggccttggtc gatgttgtgg gtggtcagca cgatcgtgcg
     5161 gtctctcgtg tggagggtct cgagcacgcc tcggagcatg cgggaggcgt gctgatcgag
     5221 gcccgtgtag ggctcgtcca ggaacatgat gtcgggatcg tgcaggagag cgcgggcgat
     5281 ggagagccgc tgctgcatgc cgcgcgaata gccggagacc cggtcgtcca tgcggccggc
     5341 aaggcccacc tcagtgagga ggtccatcgc cctcgtgcga ggatcggcga gaccgtacat
     5401 gcgcccgaag aacagaaggt tctcaagggc ggtgagatgc gcgtagagat aggtgttatg
     5461 cgagatcaag ccgatgcggt gtcgcaggag atcggcccga gccgccacgt ccacgccccc
     5521 caccgagacc gacccctcgc tgggcttgag ctgggtggag agcaccctga tgagggtggt
     5581 cttacctgct ccgttaggtc cgaaaatggt gaggaatcgc cccttggcga gagtcaggtc
     5641 gagcccgcgg agcgccggga agtggccgaa gtacttccag agcccccgca cctcgatcgc
     5701 gggttcgggg gcgtgcgtca tcggctcaac gccggtgcgg gcgtcggtcg gggagctcag
     5761 tcctgaacct ccgcgccgca ggaggcgcag aagcgttgcc cgggcttcag agtctcgccg
     5821 cactccgcac aggcgggggc ggcccccgcc ttctgccccc cgaccttccc cttccgggaa
     5881 ccggcagcgc cactcgcacc gcggtcgcga gcgtcgatag cggcgaggat ctctagggcg
     5941 tcggcacgat agcgggcgtc gagttcatgg aagtcgccct ccgacagctt gccggtcttg
     6001 aagtcgaact cgagctcctt gagcgcggag taggtggact ccttgcgtcg cagcaggacc
     6061 tcgaggtcct ccggaaggtc gaaggtctca tcatcaccga gctgctctct caacagcggg
     6121 taggcgatga agccaaccgc cagaagcgcg agcaccactc cgaacagggt acctatcacg
     6181 tcattcgctc cttaccagac ggacgcccgc agtggcaccg tctgcgactc gcgaggatca
     6241 atcgaagtca cgcagttcat cattgaggcg acgggcgatc ggatcgttcg gatcgggcag
     6301 ctcgagagcc gtcgcgcctt cggcaggtac tgtctcttcg tcgcgccgcg ccgaccacat
     6361 accgatgagg aagtatatcg ccacggcgcc cacgatcagt cccacgaagg gcatgaccca
     6421 cccgatcacg ttgaaacccg tcttcgtggg cgccgccagc accacctcgc catactggga
     6481 gacgtagtac tggaccaact cgtccgaggt cttgcccttg acgagttgtt cgtcgatgaa
     6541 ggccttggcc ggcacccccc atccgcaatt ctggtggggg cagttcgtga gcaccgcgcc
     6601 acagccgcac tgacagatga tcttgtcgct cacggaagag cggggcggcg tcgcggcgaa
     6661 ggccaccgac ggcaccagca cggccaccgc tacagccatc aatacgatga agcgtagagg
     6721 tgcccgccgc cgacgacccc gtgatccctt gtctacagag tcgggcgtcg cagccacacg
     6781 caccggaaac tcagatctca tggagtctcg cctgcctttc gtagcgggcg gccagacgga
     6841 gagagtcccg gcggtcgggc cagatcgcga gcaggccgcc gaggaccatc actatccccc
     6901 cgatccagag ccagctgacc agcgggttga tgtccacctg catattgact tcgccggtct
     6961 cggcggagta gtcgagcatc atcacataca catcgcgcga cagcgtggag atgcggtcga
     7021 tgcgcgtcca aggttgatcc ttggcgaagt agtactcgcg gatcgggtac accaccccga
     7081 cgtctttgcc gtccttcttc acgctgaagg tggcgcggcc cacctgcttc tcgtcggtgc
     7141 tgtaggtctc catgctgtcg taggtgagcg agtaggcgcc cacggagatg gtgtcgccct
     7201 tgttgaggtt ttgcgacgcc tcctgcttga aggcgtacga gccggtgacg ccgaccagca
     7261 agagtatcac cccgacatgc acggtatagc ctccgtagcg gcgcttgttg cgccagacca
     7321 gggagcccag agccctgacg atgttctcgc cgctcatctc cctgcgcacc caggcgcctc
     7381 tcccgaactc caccaggatc gcggagagca cgaaggcgga gagactgaaa ctgatgattg
     7441 cgtacacgtg cctcatgccc agtgcgacca gcagcaccgc tgcgatcgcc ccaacggcgg
     7501 ccggatacat gaagttcttg ccgagattgg ccaaggtggc cctccgccag gcgatcagcg
     7561 gacaaatacc cgtaacgaac atgagtacca ggccgatcgg agccatgacc tggtcgaaga
     7621 agggcgggcc cacggtcacc ttgttgccgg tcacagcctc gctgatgatg gggaagagca
     7681 cgccccagag caccgtgaac gccatcccca ccaggatcag gttgttgagc aagaagctgg
     7741 actcgcgaga gacgaagctg tcgagctcat tgcgcgactt gagcaggtcg aggcgactga
     7801 acaacaggtt cagcgagaag accaagatga gccccaggaa accgatgaaa aagggcccca
     7861 gactgctctg accgaagctg tgcaccgaac tgatcacacc cgagcgggtg aggaacgtgc
     7921 cgaaaatcgt caggcagaag gtaagaatga tcaagatgat gttccacacc ttgagcatgt
     7981 ccttcttctc ttggatcatc accgagtgca ggaatgcggt gccggtgagc cagggcatga
     8041 acgaggcgtt ctccaccgga tcccaggccc agtagccacc ccagccgagt gtcacgtagg
     8101 cccaccaggc tccgacgatg ttgccgatgg tgagaaagat ccacgcgatg atggtccagc
     8161 gacgcgtgga gcggatccag aagtcaccca accgcttggt gatgagcgcc cccatggcga
     8221 aggcgaaggg gatcgagaag cccacgtagc ccaggtagag ggtgaccggg tggatcacca
     8281 tccctggatt ctcgagcatc gggttgaggc cttcgccgtt ggcgggaccg ccgcccggaa
     8341 tcaccgcgaa cgggttcgat cccgctgacg tggtgagcag gatcgtgaag aacaggccca
     8401 ccaccatcaa caccgagatc acgtagggca ggatctctcg gttcttgtga cggttctgat
     8461 agacagcgat ggctgcgaag acgcccagca gcagcaccca cagcagcatc gatcccgaat
     8521 tgcccgccca aaaggccgag acagtataga accacgagag ttcgcgcgag gtgtagctcg
     8581 ccacgtactc gttgctgaag tcgcgggtga tgagagcata gaaaagagcc gcgctggcga
     8641 cgatcgcgac cccggcaaac gcgtacgtcg cgttctccgc actcttgatc cactcccgac
     8701 gccctgtccg cacgccgatc aacggtgcga cgactccata cgcagcgatc atgaggccga
     8761 gcagaagcgc ggtatacccc acctgattca tgcggaacaa ccctccgttg gtcttctggg
     8821 cgtggcttct agttgttcga tgtggaagtc tcgccggtgg cgtcggcccc cgtgcgctcg
     8881 tacttcgtgg ggcacttggc catcatgaag tcggcggcga aagtgccgtc cttgagcagg
     8941 tcgccttcca ccacgacttc gataggagtc gccgggtcgt ccttgaagct gtcgggcacc
     9001 actccggtga actccacata cagggtgctc tcgggaacgg ccttgtcgta gatctcgaag
     9061 cggatggggt ctccgatgcc gcccttctcg agcgttccgt tgagcaggtc gccggcgacc
     9121 cgcacgttca cggcctcgcc gttggccaat gcctcggtga cggtgtagta gtagaccatg
     9181 ttctccctga tggcgaagaa ggccagagat gcgatcgcgc cgacaacgac gagaccgccg
     9241 atcaggaact tcagcttctt gctgcccatg tcgccagtcc ttcctggttc atcagtgcgg
     9301 ccgcacaagg tcgcgcgccc accacgatcg atccgatcaa tacaggagac gcttcaggcc
     9361 taccccggac agaaccgtgt tggtgatctt ctccgggaac aggccgtcgg ggcattttat
     9421 cagaacctct gtggcctcga ggcccctccc ggactccatc gagccccgca ccaccgcctc
     9481 ctcatgtgcc ctcagacgtt cggggaaggg tccggtgtag cgcacttcga gcgtctctcc
     9541 gctctcacct tcgagcacga agcgagccgc gccgccttcc tgcacgacgc tgtcggctgc
     9601 cacgcgacca ccgacacgga tgtccgcggc cgcgccaccg gcattggctt cctcgacggt
     9661 gcgatagaaa tcgccgccca cggagacgaa gagcgccaac cacaccgcgg ccgccacccc
     9721 cgcgatgatc gcagcagcga ccttcagggt ccgtgacgtg agcttccgag gaccacgggg
     9781 ggccacgacc gcgctcatct ctcctccttg tgcaccgcta tacctccgta gtgcgacgtc
     9841 gcggcaatct cagccattcg agaccttccc ggacgccccg ctaatcagcg cgccgaggga
     9901 cgcctcggcc tgctccggat ctaccgccag ccaatacagg tccttctcga tcaagaagta
     9961 gtagtgggtc tggcccatcc cgtcaagggc atacccctcg accgcctggc cctcgatggg
    10021 cctcagaccc gtgaatggcg agccgcccgc ggagatacgg tccgtcattc gccgaaagag
    10081 ctcctgggcc gagtcgatgc tagggcagcg actgacccag atcgtcgcgt ggggttggcc
    10141 tccaccctcg cccccgtact gaccgaccca ggccgccgtg atcccatccc ccagcgactt
    10201 gccgtgcagc tgttcgacct gcacaagcgc cgcctcacct gtcaccgatg tgctcagacc
    10261 ccactccccc agtctggagg gaaggaggtc ggcttccgcg gacgtcgcgc cacccgcccc
    10321 ggagtctccc ggggcgtcgg ccggagtgtc cgaggccgcc atatccatac ccggcatcgt
    10381 ctggtcaccg tgcgggttca gcacgtttcc gtagaaggac cagacgcccg cggcgagcag
    10441 caccgcgagg aagacgagcg tgatcagatg acggggcctc accgcggcat cctccaacgg
    10501 ctcacgctac gccggctcac gctacgcctg gtcatccccg cactcagggc agaaacggtc
    10561 ctggcggcgc aattgcgttc cgcacgacac gcagaacttg ctcttggcat cagcgtcgga
    10621 ggcgccgcct cccgtgtcgg ccttccgccg aggtgcgacc ggtccgcccg aagcacgctg
    10681 gtcacgggac acgtgggccc gagtcgagcg ggcgcccgaa gaacgattcc gtccgttcga
    10741 cgtcgccgct gcgggccgga aggccttgta gcctccgaag acgagcgtgg caaaagccag
    10801 cgccgccaga acgatgaaca gcgcgcggtt gttgccgccg cctccggccg ccgacgtagt
    10861 gccgggctgc gcggacgcgt tgtcgtcctt ctgcacggag gggtcgttgt cgaccttgtt
    10921 gtacgcgacc ttgactgtga acggctcgcc gacgggcagg tcggggtagt cttgcgagta
    10981 gtacttgagg ccctccgagt cggtcgtgac ttggctcaga gccgggtcca gagtgaagcc
    11041 cgtcgagcgc aacggctgct gcacctcgat ggtcgtgttg gctgcggcga gcccggggcg
    11101 cagcacgaag tcgaaggcgc gggcttccgt tccggcggga aacgcatcgt agtagtactc
    11161 gaagaagatc ttgtgctgcg cctccacctt gtacgtgagc gcctgatagt cgcccttgtc
    11221 gacgagcgtg tagggcttgc aggcgtggcc gcctcctgag tcgacctcgc acgccatgcc
    11281 gatttcagcg cccttcggga tgttgtacgt cacgtcgacg gggagttgca cactgggatc
    11341 gaggtcggct tgatagatca ccaacacccg cgggtcgtcg tattcaggcc acacggacac
    11401 cttcatctga gtggccgtga tctcggcggc ctgcgccggc gtggccgttg ctccccacgc
    11461 gatcaccagg gctacggcga gcagcgacat caagaggacc gtccgcaaac cgaggcggcc
    11521 ctcccgcatg ccgagcgcgg cgacccgtga tctaccctga ttctccatgt ctctactgct
    11581 catatggctc cccttggaac gatccgctac ggctgcgaga gtcgacggtc ctcagaagtg
    11641 ccgttccggc accccggcaa cgcgttgagc gtgtttgtga agacccgcgc gtactgttgg
    11701 cgcatattac tacgccatgt agtagctgtc aaccggcggt ctcactcgct caggaatcgc
    11761 gcgcagcgcc gcactccgag cagaagcggt ccttcttgga gagccgtcct ccgcactcgg
    11821 tgcagaatcg agcggggccg ccgacattct ccgccgagtc gttccgagcc tgcctggtag
    11881 tggagcggga agaagtgggt ccgcccttgg tggaggtccg gtccgtgccg cggcccgaac
    11941 gcgaggcggg gcgctgcgac ggcagcctgc ccgcaccggc aatgggctgc ttgcggatga
    12001 acagacggta cacgatgatg ccgccgatcg cgacaaccag tacgaccaga aggaccaaca
    12061 ggtagctgct gctgcgctgt tggccgggat cactttcggg ggcggcggag gacaccgacg
    12121 ggtcggcgct ggtcttcgtg taatcgacct gcgtctggaa gatatctccg gccttcacgc
    12181 ccgagacgct cccgtaatgg aaggtgaacc cctccgcgtc ggtggtcgac tggtccagga
    12241 ccgctccggc ggagaagtct gaggccgcaa ggggctgctg cacctccacg tagagttcgc
    12301 ccacgtccgc gggcgcctgg aactgcaccg ggaacgactt ggccccgggg ccgcccacgc
    12361 ccgggtcgta gtagtactcg aagcgaagca cggggcgctt gggcacggtg accgtgacgg
    12421 cgtccattac ctcgcccggc acgatgacgg gtgtcccgag ggtgtagtcg aaggcgccgg
    12481 tctcatcgat ctcggcgatt cccgcgagcc gggcgccgcg cggcacgaag aaggtgaggg
    12541 tgtaggggaa gacggtcgtc gcgggcagcg tgacgtcaag aagcacgagt acgtcgcccg
    12601 tgtcgtactc cggccagaac tgcacaccca tctgagcggc aagaagcccg ggattgggcg
    12661 gctcgaagcc ttcgggggcg gggatcaggc ccgacaccgg tgcatccgcg gccgaggtcg
    12721 tgacggttga cgcggtcgca tcagccggtg catccgtggt ggtggtcgcg gcgtgagcca
    12781 ggcccccgaa caggagcagc gccatcgcca tcgccgtcgt gacgacgaag aacttcccta
    12841 gcgtgtgggg ccacgcgcgc agccgtggct tcgaagtggc ggagtcatac attgagaagg
    12901 gctcctgtcg ctcggttatc tacgcgggcg ccgatcgcgg cggcggcggc tgctgtgctt
    12961 cttaggcgtt cgggggctct tgccagactg cgacgatctg ccggcgcccc ggtgacccgg
    13021 tccggcgccc gctcccgacc tcgcgccggc cgaagtaact ccgccggcgg ggaatccggt
    13081 cgcccgatgc gactcgagcc accaggcgat cgcgatgccc cccgcgaaca gggcgacgct
    13141 tatccattgg gcctgagtga gtccaagcgc cacgggctgg ttcgtgcgcg cgaactcgac
    13201 gacgaaccgc tcgacgccgg ctagggctag gtatgcccag aagagggagc ccgcccttac
    13261 caaccgaggt cgcaggagca gaaggagaac ggccagtatc gccagagaca tcaggctctc
    13321 gtagatctga gtggggtgca cgagctgcgt ggtgggcggc gagcctttcg gaaaggacat
    13381 cgcccacggc aggtcggtcg gcaccccgta gtcgtc
//
LOCUS       DASRMJ010000299         4560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_276576, whole genome shotgun sequence.
ACCESSION   DASRMJ010000299 DASRMJ010000000
VERSION     DASRMJ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276576"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFD74_08235"
     CDS             <1..784
                     /locus_tag="VFD74_08235"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HZK49573.1"
                     /translation="GYQVVVREINGQFLKAGLGKIEKTLGGAVAKGKMAEAEMQAILG
                     RVKGTTTLQDFGGCDLVIEAAIENLDEKKKVFTELDAVCPPHAILSSNTSSIPIIAMA
                     TATKRPEQVLGLHFFNPVPVMKLLEIIRTIKTSEETIATARAFSDKIGKQSILAKDRA
                     GFIVNLLLIPYLLEAIRAYEMGLATVEDIDNGMMLGCAHPMGPLTLLDFVGLDTTYYI
                     ANIMFDEFKDPKYAAPPLLKQMVTAGYLGKKSGRGFYDYTKG"
     gene            788..1573
                     /locus_tag="VFD74_08240"
     CDS             788..1573
                     /locus_tag="VFD74_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZK49574.1"
                     /translation="MTYENILLDREEDIALLTFNRPKALNALNAATICELDAALDEVA
                     ADDSIKALVITGTGDKAFVAGADITEIRALPSAKAGLDMCRRGQAAFFKVEKLDKPVI
                     IAINGFALGGGCELAMTGDVRIAADTARLGQPEINLGIIPGYGGTQRLPRLVGKSAAK
                     LLIYTGDLITADEALRIGLVDKVVPASEVLATAKALAKQLAAKAPVALALCKRSIELG
                     LEVDIERGCAIEAENFGVCCATEDKAEGTAAFVEKRKPVFKGQ"
     gene            1591..3240
                     /locus_tag="VFD74_08245"
     CDS             1591..3240
                     /locus_tag="VFD74_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HZK49575.1"
                     /translation="MAATRTLLTAEDKAKYDRLTPESVVFLRSRNNKANRWVIADLLR
                     RSRYHFPNKTALVFGDRSLTYSELDDECNRVANALLDLDLQKYDRVAILAHNTLHHVL
                     TWFGTAKAGGVYLAINYLLRGKDISYSINHSEAKIFIVEDVLYDLVKDVLDEMPTVKT
                     WIWSNQGGGKPALPGFSDFDEWVRRYPADEPDTVLHIEDPVQMTYTSGTESLPKGVII
                     GNQALMAQYMGCIVDGRYDSDDINVNALPIYHCAQRDVFMNPIFWLGGTNVLMSPDLG
                     MILKNIEAYRATMFFAPPTVWIGLLRHPDFGKHDLSSLTKCYYGASIMPVEILREIIE
                     KFPKAKIYNYYGQTELAPYHTILKAEDALTKLGSAGMAGLNMETRLADDDGAEITDVN
                     VPGEICGRGPHAMMLYFKDPEKTEAAMKGGWFHSGDLGVLDEDRYIAVVDRKKDMIKT
                     GGENVATREVEETIYLDKRVQEVAVVGVFHPKWVEAVIALVVPKAGQELKEEEIAALC
                     QKHLAPFKVPKKVLIVDALPKTPTGKILKREMRQTYDDLFR"
     gene            3290..>4560
                     /locus_tag="VFD74_08250"
     CDS             3290..>4560
                     /locus_tag="VFD74_08250"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HZK49576.1"
                     /translation="MPAKLIEKTPSAYAYPLLIKHLLHAPLAHSPNQEIVYRDLVRYT
                     YREFGQRLGRLANGLASLGVKPGDTVAVMDWDSHRYLECFFAVPMMGAILHTVNVRLS
                     PEQLIHTINHTEDDVILVNAEFLPVLEQIRDRLETVKQFVVLSDAKEPPTTTVPLAAE
                     YEELLARSPASYDFPDLDENTRATTFYTTGTTGFPKGVYFSHRQLVLHTLAGLGAMGS
                     SPEQGRFHRGDVYMPITPMFHVHAWGVPYMATLMGVKQVYPGRYDPAVLLGLVQKEKV
                     TFSHCVPTILHMILGSPAIQQTDLSKWKVIIGGSTLPKALCKAALAKGIDIFTGYGMS
                     ETCPVLTLAHLSPEMVDLDEEREVEIRCKTGRPLPLVDLRIVDETMGDVPHDGKATGE
                     IVVRAPWLTQGYLKDARNSEALWAGEYLHTG"
BASE COUNT          889 a         1566 c         1429 g          676 t
ORIGIN      
        1 gggctaccag gtggtcgtcc gggagatcaa cggccaattc ctgaaggcgg ggctcggcaa
       61 gatcgagaag acgctcggcg gcgcggtggc caaggggaag atggccgagg ccgagatgca
      121 ggcgatcctg ggccgcgtca agggcacgac cacgctccaa gacttcggcg gctgcgacct
      181 cgtcatcgag gcggccatcg agaacctgga cgaaaagaag aaggtcttca cagagctcga
      241 cgccgtctgt cccccccacg ccatcctctc cagcaacacg tcctcgatcc cgatcatcgc
      301 catggcgacg gcgacaaagc gcccggagca ggtcctgggg ctgcacttct tcaacccggt
      361 gccggtcatg aagctcctgg agatcatccg cacgatcaag acgagcgagg agaccatcgc
      421 cacggcacgc gccttctcgg acaagatcgg aaagcagtcg atcctcgcca aggaccgggc
      481 ggggttcatc gtgaacctgc tcctcatccc ctacctcttg gaagcgatcc gcgcctacga
      541 gatgggcctg gccaccgtgg aggacatcga caacggcatg atgctcggct gcgcgcaccc
      601 catggggccg ctgacgctcc tagacttcgt gggcctcgac acgacctact acatcgccaa
      661 catcatgttt gacgagttca aggaccccaa gtacgctgcg ccgccgctcc tgaagcagat
      721 ggtgacggcc ggctatctgg gcaagaagag cgggcgcggt ttctacgact acacgaaggg
      781 gtgagctatg acctatgaga acatcctcct ggatcgggaa gaggacattg cactcctcac
      841 cttcaaccgg cccaaggcgt tgaatgcctt gaacgccgcc acgatctgcg agctcgacgc
      901 cgcgctcgac gaggtggcgg ccgacgactc gatcaaggcg ctcgtgatca cgggaacagg
      961 cgacaaggcc ttcgtggccg gggccgacat caccgaaatc cgcgcgctgc cctccgccaa
     1021 ggcgggcctc gacatgtgcc ggcgggggca ggcggccttc ttcaaggtgg agaagctcga
     1081 caagcccgtc atcatcgcca tcaacggctt cgccctgggc ggaggctgcg agctcgccat
     1141 gaccggcgac gtgcggatcg ccgccgacac ggcgaggctc ggccagcccg agatcaacct
     1201 cgggatcatc cccgggtacg gcggcaccca gcgcctgccg cggctggtgg gcaagagcgc
     1261 ggcgaagctc ctcatctaca ccggcgacct gatcacggcc gacgaggccc tgcgcatcgg
     1321 cctggtggac aaggtcgtgc cggcctccga ggtcctcgcc accgcgaagg ccctggccaa
     1381 gcaactcgcg gccaaggcgc ccgtggcgct cgccctttgc aagcgctcga tcgagctcgg
     1441 cctggaggtc gacatcgagc ggggctgcgc catcgaggcc gagaacttcg gggtgtgctg
     1501 cgccaccgag gacaaggcgg agggcaccgc cgccttcgtg gagaagagga agccggtctt
     1561 caaggggcag tgagggagaa aggagccacg atggccgcca cgcggactct cctgaccgcc
     1621 gaggacaagg cgaagtacga ccgcctcacc ccagagagcg tcgtcttcct gcggtcccga
     1681 aacaacaagg ccaaccggtg ggtcatcgcc gacctcctcc ggcgttcccg ctaccacttc
     1741 ccgaacaaga cggcgctcgt cttcggcgac cggagcctca cctactccga gctggacgac
     1801 gagtgcaacc gcgtcgccaa cgccctcctg gacctggatc tccagaagta cgaccgggtc
     1861 gccatcctcg cccacaacac gctgcaccac gtgctcacct ggttcggcac cgccaaggcg
     1921 gggggcgtgt acctggccat caactacctc ctgcgcggca aggacatctc ctactcgatc
     1981 aaccactccg aggcgaagat cttcatcgtg gaggacgtgc tctacgacct cgtgaaggac
     2041 gtgctcgacg agatgccgac cgtcaagacc tggatctggt cgaaccaggg aggcggtaag
     2101 ccggccctcc ccggcttctc ggacttcgac gagtgggttc gccggtaccc ggccgacgag
     2161 cccgacacgg tgctccacat cgaggacccc gtgcagatga cctacacgag cggcaccgag
     2221 tcgctgccca agggcgtgat catcggcaac caggccctca tggcccagta catggggtgc
     2281 atcgtggacg gccggtacga ctcggacgac atcaacgtca acgccttgcc catctaccac
     2341 tgcgcccagc gcgacgtgtt catgaacccg atcttctggc tcgggggtac gaacgttctc
     2401 atgagcccgg acctggggat gatcctcaag aacatcgagg cgtatcgggc gacgatgttc
     2461 ttcgcgccgc ccacggtttg gatcgggctc cttcgccacc cggacttcgg gaagcacgac
     2521 ctctccagcc tcaccaagtg ctactacggc gcctcgatca tgcccgtgga gatcctccgc
     2581 gagatcatcg agaagttccc caaggcaaag atctacaact actacggcca gaccgagctc
     2641 gccccttacc acacgatcct gaaggccgag gacgccctca ccaagctcgg gtcagccggc
     2701 atggcaggcc tcaacatgga gacccgcctc gcggacgacg acggcgccga gatcaccgac
     2761 gtaaacgtgc cgggcgagat ctgcggccgc ggcccccacg cgatgatgct ctacttcaag
     2821 gacccggaga agaccgaggc cgccatgaag ggcggctggt tccactcggg cgacctgggc
     2881 gtgctcgacg aggaccgcta catcgcggtg gtggaccgca agaaggacat gatcaagacc
     2941 gggggcgaaa acgtggccac ccgggaggtg gaggagacca tctacctgga caagcgcgtc
     3001 caggaggtgg ccgtcgtcgg cgtcttccac cccaagtggg tcgaggccgt catcgccctc
     3061 gtggtgccca aggccggcca ggagctcaag gaggaggaga tcgcggccct gtgccagaaa
     3121 cacctggcgc ccttcaaggt gccgaagaag gtcctaatcg tcgacgccct gccgaagacc
     3181 cccacaggga agatcctcaa gagggagatg cggcagacct acgacgatct gtttcggtga
     3241 gcgatcagcg atcactgatc actgaccact gacaaccagg aggcccggca tgcccgcgaa
     3301 gctcatcgag aagaccccct cggcctacgc ctacccgctc ctgatcaagc atctgctcca
     3361 cgccccgctg gctcactctc cgaaccagga gatcgtctac cgagacctgg tgcgttacac
     3421 ctaccgggag ttcggccagc ggctcggccg gctggccaac gggctggcga gcctgggggt
     3481 gaagccgggg gacacggtcg ccgtcatgga ctgggacagc caccggtacc tggagtgctt
     3541 cttcgcggta cccatgatgg gggcgatcct ccacaccgtg aacgtgcgcc tgtcccccga
     3601 gcagctcatc cacacgatca accacacgga ggacgacgtc atcctcgtga acgcggagtt
     3661 cctcccggtg ctcgagcaga tccgagaccg cctcgagacc gtgaagcagt tcgtggtcct
     3721 cagcgacgcg aaggagccgc ccacgacgac cgtgccgctc gccgccgagt acgaggagct
     3781 cctggcccga agccctgcgt cgtacgactt cccggacctg gatgagaaca cgcgcgccac
     3841 gaccttctac acgaccggaa cgaccgggtt ccccaagggg gtgtacttca gccaccgtca
     3901 gctcgtcctc cacaccctgg ccggtctcgg ggcgatgggc tcgtcgcccg agcagggaag
     3961 gttccaccgg ggcgacgtct acatgcccat cacccccatg ttccacgtcc acgcctgggg
     4021 cgtcccgtac atggcgacgc tcatgggcgt gaagcaggtc tacccgggcc gctacgaccc
     4081 ggccgtgctc ctgggcctgg tgcagaagga gaaggtcacc ttctcccact gcgtgcccac
     4141 gatcctccac atgatcttgg ggagcccggc gatccagcag accgacctct cgaagtggaa
     4201 ggtgatcatc gggggctcca ccctgcccaa ggcgctgtgc aaggcggcgc tcgccaaggg
     4261 catcgacatc ttcacaggct acggcatgtc cgagacctgc ccggtcctca ccctggcgca
     4321 cctgtccccc gagatggtgg acctggacga ggagcgcgaa gtggagatcc gctgcaagac
     4381 cggccggccg ctgccgctgg tggatctgcg gatcgtcgac gagaccatgg gcgacgtccc
     4441 ccacgacggc aaggccacgg gggagatcgt cgtgcgcgcc ccctggctca cccagggcta
     4501 cctcaaggac gcccgcaact ccgaggcgct ctgggcgggg gagtatctcc acacggggga
//
LOCUS       DASRMJ010000300        17682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_31066, whole genome shotgun sequence.
ACCESSION   DASRMJ010000300 DASRMJ010000000
VERSION     DASRMJ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 17682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17682
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31066"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFD74_08255"
     CDS             <1..613
                     /locus_tag="VFD74_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZK49577.1"
                     /translation="ALSLGGLFAGWFTPTEAGAVGAAGVFVIGVVERRLGYTQIRRAL
                     ADTTRTSAMILLLLAGATIFGRLIAVSRIPFELAEMLSSLPVPAFVIMGLILFIHLIL
                     GMFIDAMAMILLTIPIFYPLAVNTLGYDPIWFGVIIVFVVAMGVITPPVGINVYVVEG
                     VADGVRTPTIFKGIWPFLMAIIVGIVLLMLFPQIATFLPQLMG"
     gene            709..2898
                     /locus_tag="VFD74_08260"
     CDS             709..2898
                     /locus_tag="VFD74_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZK49578.1"
                     /translation="MSETRSDIRCLVPDGVTSTVCMQCPWGCGINVHIEKGAIARISG
                     NKNHPFSNGTVCPKGTAAGEVLTNPRRITSPMRRTESGWEPISWDSAYEILVEQLTRV
                     RDEYGPKALAVAIGMPVLLGGNSTVSFLRRFCDIYGTPNCFSVESICFRCQIIARILT
                     LGAYEVPDITNSACVLVWGNNPQASAPPAAARIREALEKGAKLIVIDPRATEFAAQAD
                     VHLQVRPGTDTALALSMIDVMATEGLIDREFVAAWTVGMDELAEVASDYPPTRAAEIT
                     GVPAEKIVQAARMFAGAASACIVQGTNSLDQHATGLQNSRCVAILQAITGNIDNPGGF
                     VSTSKVRLNPIRLPELMEGQPLGADRYPLFNEVWGRNFGEGQAMLLADTILSGDPYPI
                     KAMIVSASNPVITWPGGRKLERAMESLDFLAVMDLYMTPTAEKAHLFLPAATFMERVE
                     LCDYYGTLQAVPYVAMRRKMFQVGEAISDVDFWIELGKRMGYGELLPWSDAKEALDYA
                     LEPSGFTLADLDRSPDGGMPFGSVRLGHYLKKGGFATPSGKIELASATLRDLGHDAVP
                     RHRDSPEGPDSFMTAADSATAGGDAAPAGGPAAGAAAPSREPAFPLVLTTGARLLQYL
                     HSGHRDIERLAKRGRYPQAELHPDTARRYGIEDDVAVEIETRHGTVQMRARVTEAILP
                     GVVSLPHGWNDASVNDLTDDYQGDPITGYPILKSKACRIRAVAAVAE"
     gene            complement(2988..3788)
                     /locus_tag="VFD74_08265"
     CDS             complement(2988..3788)
                     /locus_tag="VFD74_08265"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZK49579.1"
                     /translation="MLLIGERLNTTRAGLQAAVETRDAGVVAAEAEAQRDAGAHLLDV
                     NCGSITAAEEPAALAWMVQAAQAAVELPLCIDSANPAALAAGLAVHQGRPLINSISGE
                     SERYRAVLPLALDYGAGVVALGMDDGGIPQDRQSALEVGTVLVKRLLADGVSLDAIYF
                     DPLVRALSTSPASIAESLELIEDLGRRFPGLHFVSGLSNVSYGLPERRHVNRAFAVLS
                     IMSGLDAVIVDPLDRTLVALIYAAEALAGRDRFCLEYIKAFKAGRLAV"
     gene            complement(3795..4418)
                     /locus_tag="VFD74_08270"
     CDS             complement(3795..4418)
                     /locus_tag="VFD74_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823746.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK49580.1"
                     /translation="MHPIAQALRDLDEPTVNRLIDEELERGTSPLELVTHLNDGMVAV
                     GRLFAEDEYFISQLIFSAEILKGVMLRLEPLLLEGDEAERGGCVVIGTVKGDIHDIGK
                     NIVATLLRGSGFDVVDMGVDVPADKFVEAVREGGAKAVCLSALLNVTYPEMKNVVETF
                     ERSGLRDQVEIVIGGAPINEQVREFAGADFYAPDAVAGVELCKKIYG"
     gene            complement(4439..5809)
                     /locus_tag="VFD74_08275"
     CDS             complement(4439..5809)
                     /locus_tag="VFD74_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK49581.1"
                     /translation="MTQELEGLYGERLGRYQAAIALEPTDRVPIAPGSNYFAEVYSGN
                     TNQETLYDPAKWVEGELEFVRAFPETDVLRDNRLWGPQLDAIGLRSYKLPGRDLAARD
                     QFQFVEREYMMADEYNLLIADPLEFMFERWFPRILGEYERPGSRRSYMAFLKSGMAQG
                     MYAQIMRDRSIRLQEVAGMPQPMTGAFVAPFDVLADTLRGLTGAIMDCFRQPDKVVAA
                     CDRLVEEMANFALRTADPLKRYPIFVPTHKPCFLSPDQFDRFYWPSFKKVLEILIGAG
                     YKVRAYLEGDWSAHWHHMLELPKGSVLCDIDNQGDIFRAKRDIGHHQCLAGGIQDSQF
                     ILGTTDQIREQVKRLCESVGVGGGFIISGGCNIPYTTKPENYRAMIEATLEFGTYDAS
                     LRPMPKQTVGPTIVDAFDYPKKMTPWTAKKQELGGAVPGDERLIQRPWDDLEAMAYAW
                     LWQWTM"
     gene            complement(6062..6715)
                     /locus_tag="VFD74_08280"
     CDS             complement(6062..6715)
                     /locus_tag="VFD74_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK49582.1"
                     /translation="MIMDRLHELVHAVGELDEQRALLIVEGALEAGDSPLEITRAGER
                     GMRVVGERYEQGVYFLSGLIMAGEIFKGIMALVKPGLQAELSGDASGRVLLGTVAGDI
                     HDIGKNIVTMALRGFGFTVDDLGVDVRPERFVEAARSFQPDVVGLSGLTSASYEAIRL
                     AIEALRAAGEPVGSTPIIIGGAAVNQQVADFTGADHWTTDAMEGVRICERLRSPSEG"
     gene            complement(6712..7551)
                     /locus_tag="VFD74_08285"
     CDS             complement(6712..7551)
                     /locus_tag="VFD74_08285"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZK49583.1"
                     /translation="MPEEPVKTGSAPRGSRAPVAGGAAPSPSRSGGPPRKRNPYEPAY
                     AHIVNTVSDRIARGEYRSGDRLPSESQFCAEFSVSPMTLRRAVNMLVDRGLVITAQGR
                     GTFVRPLDLGEATFKLQQLTSRWREASTEVRLVEASIVPAAERVAEILGLALGEKTVY
                     LRRLFVEDGHPFMYHAEHVVYDARRPLVESQLQITSLEGVLKSAGGEGFARARLTIQA
                     VNLDETIAGHLDRPPGTAAFCLEHVFESFDGVALSWGSFFFLSDEFQLVTYMGAEAGG
                     GGS"
     gene            complement(7716..7982)
                     /locus_tag="VFD74_08290"
     CDS             complement(7716..7982)
                     /locus_tag="VFD74_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49584.1"
                     /translation="MSEWTRGPSASPGRPDDPYALLLAELDESVRALKQAARRLRHSA
                     QGIPAVECNADRILASTRMLEINISDLLRDPDSLASFDRDSRVE"
     gene            complement(7979..9325)
                     /locus_tag="VFD74_08295"
     CDS             complement(7979..9325)
                     /locus_tag="VFD74_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HZK49585.1"
                     /translation="MHSTRYWNPILETMPRERLREVQLRKFRNIVTWAYENSLFHRSL
                     YREAGFEPGDLHTLEDAALVPKVDKSHMRGIQRKDPFPYGDMLCVPLDRVTEFRQTSG
                     TTGQAVYQADTWQDWEWWAECWSYLLYSQGYRAHDRVFLPFGYNIFVAFWAAHYAAEK
                     MGCEVVPGGVLDTEARILKMQELQTTAMMATPTYVLGMAEKARAMGIDPRTDLSVCRI
                     TCAGEPGASIPATKHRIEEAWGAKVFDHCGATEIGAWGFECEEQSGGIHLNEGLFLAQ
                     VEDLETGELIEEPGRKGRLVITALDRIAMPCVRFDSKDVVEWADGLCSCGRTFRLFKG
                     GVVGRADDITKVKGVLLAPSAIEEVVRSFEELGDEYEVTVVKRGDLDDISLKVEPASG
                     FDLSGERLDGFLRSFKDQLRLKTNLGYKVEVVEAGSLPRYALKAKRFKDLRKVNGA"
     gene            complement(9339..9989)
                     /locus_tag="VFD74_08300"
     CDS             complement(9339..9989)
                     /locus_tag="VFD74_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZK49586.1"
                     /translation="MKPLIVGISGASGTVYGVRLLEVLKETPIETHLVMTASTRKVVE
                     LETGYTAAQIEALADWVHDIRDIGAPIASGSFKTRGMVVAPCSMKSLSAIAHSYNENL
                     LVRAADVVLKERRKLVLVTRETPLHLGHLRLMQQVVETGGVILPPMPSFYHRPETIAD
                     IVDQTVGKVLDQFDIEHDLFRRWEGTRLRMLEREAERLSVDVSAGPGALHIDHDAR"
     gene            complement(10081..11424)
                     /locus_tag="VFD74_08305"
     CDS             complement(10081..11424)
                     /locus_tag="VFD74_08305"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HZK49587.1"
                     /translation="MAYQDLRAAVAALRADGRLTEVTAPVALRHEVAAVIAAASRKGG
                     HALLFSNLIGHSVPIVANMVHSRQVLADAMGVPLDELIERFTERVMAPIDAVAATEAP
                     VLDVVHDDVDLAALMPILTHYEGDAGPYLSTGMANVRNPATGVVERTICRMQLRGRDR
                     LGVALVNPPLGDTYRKYGRDTEMELAVVVGLEPATFMAAALPAPPGADKMGYAGGLRG
                     EPVELTDGGLLALPVPARAEFLLEGVLVPGEEQDDGPFGEISGYSMAFPSTPTLHVRR
                     VSHRRDPLYHALLPTGPEGDLITGLTVEAAIAPQLRRVYSFARGITFVPGTFGMSLVV
                     QVAQADKGRVRGLLYHLLSLERIKKVVLVAEDVDPTDPLEVEWAVVTRVQPDEDVVTI
                     DGVYAHPIDPSYHPGQRTSRIGIDATGFERIGGRERVRVPDAAAVRAAEILEGAC"
     gene            complement(11434..11691)
                     /locus_tag="VFD74_08310"
     CDS             complement(11434..11691)
                     /locus_tag="VFD74_08310"
                     /inference="COORDINATES: protein motif:HMM:NF021199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit gamma"
                     /protein_id="HZK49588.1"
                     /translation="MIEYDTFFMGEPSDVLQDEVTEIVIRDLTPGRHKYRCAYAEVRL
                     SSDADKYPHRLWPRLGRGQWVGEAWSVEVVREVNKIPEAWR"
     gene            complement(11777..13222)
                     /gene="ppcA"
                     /locus_tag="VFD74_08315"
     CDS             complement(11777..13222)
                     /gene="ppcA"
                     /locus_tag="VFD74_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit alpha"
                     /protein_id="HZK49589.1"
                     /translation="MANKYYKDNREFIKGLDEAGLLVTVEQEVDWDLEMGAVVRRVCE
                     MKGPAPYFRNIKDYPGFEALGAPLATHSKLALALGLAPDTPIPDIGRSYTERTGGTPI
                     DPVEVSRASAPCKQNVILGDDVDLFDLPGPMVHDGDGGRYLATWHMVVSREPDSGDIN
                     WGMYRQMVFDEKTMVGPVLPFSDMGKMFCNKYVPRNQPMPFATVIGMDPLSGIAACAP
                     SPIPEDQFTGMMMGEPVELVKCETSDLMVPAHAEIVIEGEVLPGINMLEAPFGEYTGY
                     RTSPREPRAVYRVKAITYRDRPILAVSNMGIPTDEGQLLRSFSLGLEMEKLLRSQGIP
                     ITGVYMLPESTHHLVVVGTRPAYAGIASQIAQLVFGSKLGPWFHQVMVVDNTTDIYNV
                     NEVIHAFSTRCDPANGIHIYRNSAGTPLNPYSSPADRKVGRGSKVLFDCLFPLDWSRA
                     DTPILVSFATAYPKEVQERVLEQWSNYGFGE"
     gene            complement(13222..13764)
                     /locus_tag="VFD74_08320"
     CDS             complement(13222..13764)
                     /locus_tag="VFD74_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZK49590.1"
                     /translation="MEKDRDAAVERAAKIKLVVLDVHGVLTSGMILYNDEGMKFQAFH
                     HDDGFGANCLMMLGVDVAFMTRKSKIVELRAAEIGVKNVFQAKNKLAKLEEVAGEMGI
                     GFDEICFVGDEIIDMGVMKEVGLAVSPANGVAEVQAISHWVTGRSGGEGMLRELAEFI
                     LRAQDKWQGLVDKVSSKGWG"
     gene            complement(13837..15252)
                     /gene="ppcB"
                     /locus_tag="VFD74_08325"
     CDS             complement(13837..15252)
                     /gene="ppcB"
                     /locus_tag="VFD74_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit beta"
                     /protein_id="HZK49591.1"
                     /translation="MDLRDFIAQCEKQGDLKRVTAEVDWDLEMSHIAKLVEEKQGPAL
                     LFENIKGHDKSQSVLFGVYSNTRRLATVLGRRADMTMVELSHEWMKLSVGEVIRAKEL
                     ETGPIFENVIEGDDIDIFKIPSPKFYEQDGGRYIGTACFMVVRDPETGEINLGTYRSQ
                     VLDKKTVGAQILKGKRGDRILQKYRKRGEKMPVCMIVGCDPLLMLAGSAMVENASEYD
                     VVGTLRGEPVEIVTAPLTGLPIPATAEWVLEGYIDGDDLRPEGPFGEYTGYYTEELYH
                     PVPKPAIQVERIYHRNDPIFLAASVGRPVNDNHMMLAFVRNATLWTELTKMGIKGIES
                     VYMPPEACGRFWAIVSVKQMYPGHSNQVAAAVIASNTCTYGLKGLIIVDHDIQADDLQ
                     RVWWALATRYDAARGTQIINRGRSTPLDPALDPGNKFITSRIILDATVPFEWKEKPVE
                     VKMSPAVLESIKARWSELGLD"
     gene            complement(15563..16627)
                     /locus_tag="VFD74_08330"
     CDS             complement(15563..16627)
                     /locus_tag="VFD74_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZK49592.1"
                     /translation="MSDKESRILFWYEELEGSDFPLVGKKNANLGEMLKGGIRTSPGF
                     ALTLHANDLFIIETGIKDELAAYLESLGEATLEACSQAGDVAVKLITEATVPAAIVEE
                     ACGAYQRLCERAGVQNVPVAVRSSGAVSMPGQMETYLNIRGEKDLVEYIKKTWASAYH
                     VEAITYRLNKGLGFLLNIGVGIPKMVNSRVSGVSFTLNPLNGDRSKIAVDVSYGLGEA
                     VVSGLVTPDNYLVDKVTLSTIKTTLGSKELQCVYNEGGSDIKTVDVCAEDRGRPCVAP
                     EELKEIVRVSKAIDKYYGKPYDIEFAIDADLPFPDSVIILQVRPESVWSKKAEQGKTE
                     QKKDALDRIVSQLVTGVRIK"
     gene            complement(16696..>17682)
                     /locus_tag="VFD74_08335"
     CDS             complement(16696..>17682)
                     /locus_tag="VFD74_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HZK49593.1"
                     /translation="ARYPWFYISTGTGWYHHHYSWNDNLDVPFASIVMHLEAIKAGKE
                     VGRPTEKLIEESRSIVEEYRALIDNDADREAFDQTLAISQRVFPYVEDHLFYVEHWFH
                     SIFWNKVRQVSDILTDAGFFKDREDIWLLKRGEIRDALWDHVTSWATGTIGRGPSYWP
                     KEIEWRKGVMEKFQAWSPPPALGTPPDVVTEPFSIVLWGVTTSVLKNWLKGMGTGGDA
                     DATELAGSPGSSGVVEGRARVIKSAEELGSLLEGEILVATTTSPSWAPAFTKIAGAVT
                     DVGGAMCHAAIVCREYGLPTVVGTGRGTTFIKTGDLVRIDGDAGTVTIVERA"
BASE COUNT         2917 a         5993 c         5660 g         3112 t
ORIGIN      
        1 cgccctctcc ttgggaggac tgttcgccgg ttggttcact cccaccgagg ctggggccgt
       61 gggggccgcc ggtgtgttcg tcatcggcgt cgtggagcgg cgcctaggct acacgcagat
      121 ccgcagggcg ctcgccgaca ccacacggac ctcggccatg atcctgctgc tcttggcagg
      181 cgccaccatc ttcggccgcc tcatcgcggt gagccgcatc cccttcgaac tcgcggagat
      241 gctcagctca ctgcccgttc ccgcgttcgt catcatgggg ttgatcctct tcatccacct
      301 gatcctgggc atgttcatcg acgccatggc catgatcctg ctcaccatcc ccatcttcta
      361 cccgctggct gtgaacaccc tgggatacga ccccatctgg ttcggcgtca tcatcgtgtt
      421 cgtggtcgcc atgggtgtga tcactccccc ggtgggcatc aacgtctacg tcgtcgaagg
      481 agtagccgat ggggtgcgca cgccgaccat cttcaagggc atctggccct tcctcatggc
      541 catcatcgtc ggtatcgtgc tgctgatgct cttcccgcag atcgcgacgt tcctgccgca
      601 actgatggga tagacttggt ccacgtagag acttggttcg agtagaggcg gcttgatcct
      661 gcgccgccgg gcgcggcgca gatcggctga gcgagagacg gtgaggcgat gagcgagacg
      721 aggtccgaca ttcggtgcct ggtgcccgac ggcgtgacga gcactgtctg catgcagtgc
      781 ccttggggtt gcggcatcaa cgtgcacatc gagaagggcg ccatcgcccg catctcgggc
      841 aacaagaacc acccgttcag caatgggacc gtgtgcccga aaggcacggc cgccggtgag
      901 gtgctgacca acccccggcg gatcacgagc cccatgcgca gaacggagag cggctgggag
      961 cccatatcct gggactccgc ctacgagatc ctcgtggagc aactcacgcg cgtcagagac
     1021 gaatacgggc ctaaggcgct ggccgtggcc atcgggatgc cggtgctcct cggcggaaac
     1081 tccacggtga gcttcctgcg acgcttctgc gacatctacg gcacgccgaa ctgcttctcg
     1141 gtggagagca tctgcttccg ctgtcagatc atcgcgcgga tcctcaccct cggggcatac
     1201 gaggtgcccg acatcacgaa ctcggcctgc gtgctggtgt ggggcaacaa cccgcaggct
     1261 tccgcaccgc cggcggctgc gcgcatccgc gaggcactcg agaagggcgc gaagctcatc
     1321 gtcatcgatc cgcgcgccac cgagttcgcg gcgcaggcag acgtccacct tcaggtgcgc
     1381 ccgggcacgg acacggcgct cgccctgagc atgatcgacg tgatggcgac cgaggggctc
     1441 atcgaccgcg agttcgtcgc ggcctggacc gtcgggatgg acgaactggc ggaggtcgcc
     1501 tcggactacc cgcccacgcg ggcggccgag atcaccggcg tgccggcgga gaagatagtg
     1561 caggccgctc ggatgttcgc cggggctgcg tccgcgtgca tcgtgcaggg caccaactct
     1621 ctcgaccagc acgccacggg cctgcagaac tcacgctgcg tggccatact ccaggccatc
     1681 accggcaaca tcgacaaccc cggcgggttc gtcagcacct cgaaggtgcg cctcaacccg
     1741 atccgcctgc ccgaactgat ggagggccag ccgctcggcg ccgaccgcta ccccctcttc
     1801 aacgaggtgt ggggccgcaa cttcggcgaa ggccaggcca tgctgctggc cgacaccatc
     1861 ctgagcggag atccctatcc catcaaggcc atgatcgtct ccgcctcgaa ccctgtgatc
     1921 acctggccgg ggggacggaa gctcgagagg gcgatggaga gcctcgactt cctggcagtc
     1981 atggacctgt acatgacccc caccgcggag aaggcccacc tcttcctgcc ggcggcgacc
     2041 ttcatggagc gcgtagaact ctgcgactac tacggcacgc tgcaggccgt gccgtatgtg
     2101 gccatgcgtc ggaagatgtt ccaggtgggc gaagcgatct ccgacgtcga cttctggatc
     2161 gaactcggca agcgcatggg gtatggagaa ctcctaccgt ggtccgacgc caaggaggct
     2221 ctcgactacg ccctggaacc gtcgggcttc accctcgcgg acctcgaccg gtcgcccgac
     2281 ggaggcatgc ccttcggttc cgtgcggctc ggccactacc tgaagaaggg cggcttcgcg
     2341 accccctcgg gcaagatcga gctggcctcg gccaccctgc gcgatctggg tcacgatgcg
     2401 gtcccacgcc atagggactc gcctgagggg cccgacagct tcatgacggc ggcggactct
     2461 gcgaccgccg gcggcgatgc ggcgcccgcg ggcggcccgg ccgccggcgc cgccgcaccc
     2521 agccgagagc cggccttccc gctggtgctc accacggggg cgcgtctgct ccagtacctt
     2581 cactcgggac accgcgacat agagcggctg gccaagcgtg ggcgctaccc gcaggccgaa
     2641 ctccaccccg atacagcgcg ccgatacggc atcgaggatg acgtggccgt ggagatcgag
     2701 actcgacacg ggacggtgca gatgcgcgcc cgcgtcaccg aggcgatttt gcccggggtg
     2761 gtgagcctgc cccacggatg gaacgacgcg agcgtcaacg acctcaccga cgactatcag
     2821 ggcgacccta tcaccggcta cccgatcctc aagtcgaagg cctgccggat ccgggccgtc
     2881 gctgccgtcg cggagtaggg gcgacggggc aggcggcagt caccgagcag gggcggccac
     2941 cgagtaggcg gctccccagc ggcgaagggc gccgtcgctg ctcagatcta gaccgccagc
     3001 ctacccgctt tgaacgcctt gatgtactcg agacaaaagc gatctctgcc cgcgagggct
     3061 tcggccgcgt agatgagagc caccagtgtc cggtcgagcg ggtccacgat cacggcgtcg
     3121 agcccactca tgatgctgag gaccgcgaac gcacgattca cgtggcgccg ctcgggcaac
     3181 ccgtacgaca cgttgctgag acccgacaca aagtgcaggc cggggaaacg tcgcccaagg
     3241 tcttcgatca gctccaggga ctccgcgatg gaggcggggc tggtgctgag cgcccgcacc
     3301 agcgggtcga agtagatcgc gtcaagtgat actccgtcgg cgaggaggcg cttcacgagc
     3361 accgtcccca cctcgagcgc gctctgccta tcctggggga tgcccccatc gtccatgccg
     3421 agcgccacga cgcccgcccc atagtcgagc gccagtggaa gaacggcgcg gtagcgctcc
     3481 gactcgccac tgatggagtt gatgagaggc cggccctggt ggaccgccag cccggccgcc
     3541 agcgccgcgg ggttggcgct gtcgatgcac agaggcagct cgacggccgc ctgggccgcc
     3601 tgcaccatcc acgccagcgc cgcgggttcc tccgccgccg tgatcgagcc acaattgacg
     3661 tcgagaagat gggccccggc gtcgcgctgc gcctcagctt cggccgcaac gaccccggcg
     3721 tcgcgggtct ccactgcagc ctgcaggccg gcgcgcgtgg tgttgaggcg ctctccgatc
     3781 aacaacatcc gaggctaccc gtagatcttc ttgcagagtt ccactcccgc gacggcgtcc
     3841 ggagcgtaga agtcagctcc ggcgaactcg cgcacctgct cgttgatggg tgccccaccg
     3901 atgacgatct ccacctggtc gcgcagcccg ctgcgctcga acgtctccac cacgttcttc
     3961 atctcaggat aggtcacgtt gagaagggcg ctcaagcaca ccgccttggc accgccctca
     4021 cgcaccgcct ccacgaactt gtcggcgggc acgtcgacgc ccatgtccac cacgtcgaaa
     4081 ccggacccac gcaggagcgt tgccacgatg ttcttgccga tgtcgtggat gtcgcccttg
     4141 accgtcccga tgaccacgca tccccctcgc tcggcttcgt ccccctcgag caacagcggc
     4201 tcgagccgaa gcatgacccc cttcaagatc tcggcggaga agatgagctg actgatgaag
     4261 tactcgtcct cggcgaagag gcggccgacc gcgaccatgc cgtcgttcag atgggtcacc
     4321 agctccaagg gggatgttcc ccgctcgagc tcctcgtcga tcagccggtt gaccgttggt
     4381 tcgtcgagat cgcgcagcgc ctgcgctatt ggatgcattc gtacgccccc cgtttccatc
     4441 acatcgtcca ttgccagagc cacgcgtacg ccatggcttc gagatcatcc cagggcctct
     4501 ggataaggcg ctcgtcccct gggacggccc ctcccaactc ctgcttcttc gccgtccaag
     4561 gagtcatctt cttgggatag tcgaacgcat ccacgatggt gggcccgacc gtctgtttgg
     4621 gcatgggtct gaggctcgcg tcgtacgttc cgaactcgag cgtcgcttcg atcatcgctc
     4681 ggtagttctc gggcttcgtg gtgtagggga tgttgcagcc cccgctgatg atgaagccgc
     4741 cccccacgcc caccgattca cacagacgct tcacctgctc gcgtatctgg tcggtggttc
     4801 ccaagatgaa ctgcgagtcc tggatcccgc cggccagaca ctggtggtgt ccgatgtccc
     4861 gcttggcccg gaagatgtct ccctggttat cgatgtcgca gagcacgctg cctttgggga
     4921 gttcaagcat gtggtgccaa tgcgcactcc agtcgccctc cagataggcc cgcaccttgt
     4981 agcctgcccc gatgaggatc tccaggacct tcttgaacga cggccagtag aagcggtcga
     5041 actggtcggg cgagaggaag cacggtttgt gcgtagggac gaagatcggg taccgcttga
     5101 gcgggtcggc cgtgcgcaac gcgaagttgg ccatctcctc caccagccgg tcacaggccg
     5161 ccacgacctt gtccggctgg cggaagcaat ccatgatcgc acccgtgaga ccccgcagcg
     5221 tgtcggcgag cacgtcgaag ggcgccacga aagccccggt catcggttgc ggcatgccgg
     5281 cgacttcctg cagacggatc gagcggtcgc gcatgatctg cgcgtacatg ccttgggcca
     5341 tgccgctctt cagaaaggcc atataggagc gcctcgaacc cggacgctca tactcgccga
     5401 ggatgcgcgg gaaccaccgc tcgaacatga actcgagcgg atcggcgatg aggaggttgt
     5461 actcgtcggc catcatgtac tcgcgctcga cgaactggaa ctgatcgcgc gcggcgaggt
     5521 cccgaccggg aagcttgtag gagcgcaggc cgatggcgtc gagctggggg ccccagagac
     5581 gattgtcgcg tagaacgtcg gtctcgggga aggcacggac gaactcgagc tctccctcga
     5641 cccacttcgc cggatcgtag agcgtctcct gattggtgtt gcccgagtac acctcggcga
     5701 agtagttgct gccgggagcg atgggcaccc ggtcggtggg ctcgagcgcg atggccgctt
     5761 ggtagcggcc caaccgctcg ccgtacagtc cctccagctc ctgagtcaca ccggcccccc
     5821 cgggttgttc cgacactcgg cgcgcaatgc gcggtccgtc cgcggcggcg cgtggcgcgt
     5881 ggcccggagt cgcgtggcat ctccggcacg tggcgcgtgg cgcgggcgat gcgtcgcgcc
     5941 ctcgcacctg gcgcagcgca cttccaatat atagttgtat atagaagatg tcaacgccag
     6001 gctgattcgc gcagacggat gccgcgggtg cctacaaaaa cgggcgccgg ggcgcctacg
     6061 ctcagccttc ggacggactc ctcagccgct cgcagatgcg cacgccttcc atggcgtcgg
     6121 tggtccagtg gtcagccccc gtgaagtcgg ccacctgctg gttcacggcc gcgcccccga
     6181 tgatgatcgg ggtgctcccg accggctccc ccgccgcacg caaagcttcg atggccagcc
     6241 ggatggcctc gtaggaggca gaagtgaggc cggatagtcc gaccacatcg ggctggaacg
     6301 agcgggccgc ctcgacgaat cgctccggtc ggacgtccac tccaagatcg tcaacggtga
     6361 agccgaaccc ccgaagggcc atggtgacga tgttcttgcc tatgtcgtgg atgtcgcccg
     6421 caaccgtccc gagcagcacc ctgccggacg cgtcccccga aagctccgcc tgaagcccgg
     6481 gtttgaccaa tgccatgatg cccttgaata tctcccccgc catgatcagg cccgagagga
     6541 agtacacgcc ctgctcgtag cgctccccga cgacacgcat gccgcgctca cccgcgcgag
     6601 tgatctcgag cggactgtcg cccgcttcca gggccccctc gacgatgagc agcgcccgct
     6661 gctcgtcgag ttccccgaca gcgtgcacga gttcgtggag cctgtccatg atcatgaccc
     6721 gccgcctccc gcttcggcgc ccatgtaggt gaccagctgg aactcgtcgg agaggaagaa
     6781 gaaggagccc cagctcagag caacgccgtc gaagctctcg aacacgtgct caaggcagaa
     6841 ggccgcggta cctggcggac gatcgaggtg gcccgctatg gtctcgtcga gattgacggc
     6901 ctggatcgtt agccgagccc gcgcgaaccc ttcgccgccg gccgacttca ggacaccctc
     6961 cagcgacgtg atctgcagtt gcgactcaac gagcggccgc cgggcgtcgt agaccacgtg
     7021 ttcggcgtgg tacatgaagg ggtgcccgtc ttccacgaac aggcgcctca ggtaaacggt
     7081 cttctctccc aaggcaagac ccaggatctc ggcgacccgc tccgccgcag ggacgatgga
     7141 cgcctctacc aaccgcactt ctgtcgaggc ctcgcgccac cgcgaggtca gttgctgcag
     7201 cttgaaggtc gcctcgccca ggtcgagcgg gcggacgaag gtgccgcgac cctgggcggt
     7261 gatcacgagt ccgcgatcca cgagcatgtt caccgcgcgg cgcagtgtca tcgggctcac
     7321 cgagaactcc gcgcagaact gggattcgga cgggagtcgg tcaccggagc gatactcgcc
     7381 acgcgctatg cgatcgctca cggtgttcac gatgtgggcg taggcgggct cgtaggggtt
     7441 ccgcttgcgc ggggggccgc cactgcgcga cggagacggc gcggcaccgc cggcgaccgg
     7501 cgcccgcgag ccgcgcggag cgctacccgt cttcactggt tcttccggca ccccgatccc
     7561 tcccggcgtc gtcccctgct cccgccgcac cgcaccgcga acgcactacg gttccctata
     7621 ggaacataca atacacgacc gggagcccga gcggccgccg agatcgccgc cgccgacgtg
     7681 gcgccgcgaa ccgagggccg tagccgcccc gcccgctact cgacccggct gtcccgatcg
     7741 aacgaggcca ggctgtcggg gtcgcggaga aggtcggaga tgttgatctc gagcatgcgg
     7801 gtgcttgcga ggatacggtc ggcgttgcac tccaccgccg gtatgccctg cgccgaatgt
     7861 ctcagccgcc gcgcggcctg cttcagggcc cgcacgctct catcgagctc ggccagaagc
     7921 agcgcgtagg gatcgtcggg tctgcctggg gaagcggaag gtccgcgcgt ccactcgctc
     7981 atgcgccgtt caccttcctc aggtccttga acctcttcgc tttcagcgcg tatcgcggca
     8041 gagaccccgc ctccacgacc tccaccttgt agcccaggtt ggtcttcagc cgcagctgat
     8101 ctttgaagct cctgaggaag ccgtcgagtc tctcgccgga gagatcgaac ccagacgcgg
     8161 gctcgacctt cagcgagatg tcgtcaagat cgccgcgctt gaccacggtc acctcatact
     8221 cgtcgccgag ttcctcgaac gagcgcacca cctcttcgat ggcgctcgga gccaggagga
     8281 cccccttcac cttggtgatg tcgtcggcgc gacccacgac gccgcccttg aacagccgga
     8341 aggtgcgccc gcacgagcag aggccgtcgg cccactccac cacgtctttg gagtcgaaac
     8401 gcacacaggg catggcgatg cggtcgagcg cggttatgac gaggcgcccc ttgcggccgg
     8461 gttcctcgat cagctcgccc gtctccaggt cttctacctg ggccagaaac aggccctcgt
     8521 tcaggtggat gccgccggac tgctcttcac actcgaagcc ccaagcgccg atctcggtgg
     8581 cgccgcagtg gtcgaacacc ttggcgcccc acgcctcttc gatgcggtgc ttggtggcgg
     8641 ggatgctggc gcccggctcg ccggcgcagg tgatgcgaca gacgctcagg tcggtgcgcg
     8701 ggtcgatgcc catggcgcgc gccttctcgg ccatgccgag cacgtaggtg ggcgtggcca
     8761 tcatcgcggt ggtctgaagc tcctgcatct tgaggatgcg ggcctcggta tcgagcaccc
     8821 cgccgggcac cacctcgcag cccatcttct cggcggcgta gtgggccgcc cagaaggcta
     8881 cgaagatgtt gtagccgaag gggaggaaca cccgatcgtg agcccggtag ccctgcgagt
     8941 agagcaggta gctccagcac tcggcccacc actcccagtc ctgccacgtg tcggcctggt
     9001 acaccgcttg gccggtggtg cccgatgtct ggcggaactc ggtgacccga tcgagaggca
     9061 cgcacagcat gtcgccgtat gggaagggat ccttgcgttg gatgccccgc atatgtgact
     9121 tgtcgacctt gggcaccagc gccgcgtctt cgagggtatg caggtcgccg ggctcgaagc
     9181 cggcctcgcg gtagagcgag cgatggaaca gcgagttctc gtacgcccag gtgacgatgt
     9241 tgcggaactt gcgcagctgc acctcgcgca gtcgctcacg cggcatggtc tccaggatgg
     9301 ggttccagta gcgggtcgag tgcatcgggg cggacacctc agcgagcatc gtgatcgatg
     9361 tgtaatgctc ccgggcccgc agacacgtcg acggatagcc tctcggcttc gcgttccagc
     9421 atgcgcagac gcgtcccttc ccaccgtcgg aacagatcgt gttcgatgtc gaactggtcg
     9481 agcaccttgc ccaccgtctg gtcgacgatg tcggcgatgg tctcgggtct gtggtagaag
     9541 ctgggcatgg gaggcaagat gaccccaccg gtctcgacta cctgctgcat gagccgcagg
     9601 tggcccaggt gcaggggtgt ctctcgcgtg accagcacca gcttgcgcct ctccttcaag
     9661 acgacgtcag ccgcgcgcac caacaggttc tcgttgtagc tgtgcgcgat ggccgagagg
     9721 ctcttcatgg agcacggagc caccaccatg ccccgggtct tgaacgaccc ggaggcgatg
     9781 ggagcgccga tgtccctgat gtcgtgcacc cagtcggcca gcgcctcgat ttgcgccgcg
     9841 gtgtagccgg tctccagctc cacgaccttg cgcgtggacg ccgtcatcac caagtgggtc
     9901 tcgatcgggg tctctttcaa cacctcgagc agccgcactc cgtagaccgt gccggatgcc
     9961 ccggagatgc ctactatcaa tggcttcatg cgactcctcc ctcgcagcgg gcgcagcccg
    10021 ggcgattgcg ccggtgatcg aagcgacgcc caattcctct cccgcggatc gtcccccaga
    10081 ttagcacgcg ccttccagta tctcagcggc tcggaccgcc gctgcgtcgg ggacgcgcac
    10141 ccgctcgcgt ccgccgatgc gctcgaagcc ggtggcgtcg atgccgatcc gggaggtgcg
    10201 ctgtccgggg tgatacgacg ggtcgatggg gtgggcgtac acaccgtcga tggtcacgac
    10261 gtcctcgtca ggctgcaccc gggtgacgac ggcccactcg acctccagtg gatcggtcgg
    10321 gtcgacgtcc tcggccacca ggaccacctt cttgatgcgc tccagtgaga gcagatggta
    10381 gagaaggccg cggacccggc ccttgtcggc ctgggccacc tgcaccacga gactcatccc
    10441 gaatgttccg gggacaaaag tgatgccccg agcgaacgag tacacccggc gcagctgcgg
    10501 cgcgatggcg gcctccaccg tcaacccggt gatgagatcg ccttccgggc ccgtgggcag
    10561 gagggcgtgg tagagagggt cgcggcgatg gctcacccgc cgcacgtgaa gcgtgggtgt
    10621 ggaggggaag gccatggagt agccgctgat ctcgccgaag ggcccgtcgt cctgctcctc
    10681 gcccggcacg agcacgccct cgagcaggaa ctcggcccta gccggcaccg gtagggcgag
    10741 cagccctccg tcggtgagtt cgacgggctc gccgcgcagg ccgcccgcgt agcccatctt
    10801 gtcggcgccc ggcggagccg gcagcgccgc ggccatgaag gtggcgggtt cgaggccgac
    10861 caccaccgcc aactccatct cggtgtcgcg gccgtacttc cggtaggtgt cgcccagagg
    10921 cgggttgacc agggccactc cgagccggtc gcgcccccgc agctgcatgc ggcagatggt
    10981 cctctcgacg actcccgtgg cggggttgcg cacgttggcc atgcccgtgc tgagataggg
    11041 accggcgtcg ccctcatagt gcgtgaggat gggcatgagg gccgccaggt cgacgtcgtc
    11101 gtggacgacg tcgagcacgg gtgcctcggt cgccgccacc gcatcgatgg gagccatgac
    11161 cctctcggtg aaccgttcta tcaactcgtc gaggggaacc cccatggcat cggccagcac
    11221 ctgccggctg tgcaccatgt tggcgacgat cgggaccgag tggccgatca ggttgctgaa
    11281 gaggagggcg tggccgccct tccgcgaggc ggcggcgatc accgccgcca cctcatgtct
    11341 cagagccacc ggtgccgtca cctcggtcag gcggccgtcg gcgcgcagcg ccgcgacggc
    11401 cgccctcaga tcttgatatg ccacgaagcc ctcctagcgc caggcttcgg ggatcttgtt
    11461 cacctcgcgc acgacctcga ccgaccaggc ctctccgacc cactggccgc ggccaaggcg
    11521 cggccacagc cggtggggat acttgtcggc gtcgctggac aggcgcacct ccgcgtaggc
    11581 gcagcggtac ttgtgccgcc ccggcgtcag atcgcggatc acgatctccg tgacctcgtc
    11641 ctggagcacg tcgctgggct ctcccatgaa gaaggtgtcg tattcgatca tctcgtctcc
    11701 tctcggggcg catccgcttc cggtagcact gctcgccgaa ccgccggcac cgctgccggc
    11761 cgaccctgcg gcgccatcat tcgccgaaac cgtagttgct ccactgctcg agcactcgtt
    11821 cctggacctc tttcggatac gccgtggcga acgacaccag gatgggagtg tccgcacgac
    11881 tccagtcgag cgggaacagg caatcgaaca gcactttgga gccgcgcccc accttccggt
    11941 ctgcgggcga ggagtagggg ttcagcggcg tgcccgccga attgcggtag atgtggatgc
    12001 cgttcgccgg atcacagcgg gtggagaaag cgtggatcac ctcgttgacg ttgtagatgt
    12061 cggtggtgtt gtccacgacc atcacctggt ggaaccaggg tcccagcttg ctcccgaaca
    12121 ccagctgggc gatctgcgac gcgattccgg cgtaggcggg ccgggtgccg accaccacca
    12181 ggtggtgcgt cgactccggc agcatgtaca cgccggtgat ggggataccc tggctgcgca
    12241 gcagcttctc catctcgaga cccagcgaga aggagcgcag gagctgcccc tcgtcggtgg
    12301 ggatgcccat gttggagacg gccaggatgg gccggtcgcg gtaggtgatg gccttcacgc
    12361 gatacacggc gcggggctcg cgcggcgaag tgcggtaacc ggtgtactcc ccgaagggag
    12421 cctccagcat gttgatgccg ggtagcactt cgccctcgat gacgatctcg gcgtgggcgg
    12481 ggaccatcaa gtcgctggtc tcgcacttca ccagctccac cggctcaccc atcatcatgc
    12541 ccgtgaactg atcctcgggg atgggcgaag gagcgcaggc cgcaatgccg ctcagcggat
    12601 ccatgccgat gaccgtggcg aagggcatcg gctgattgcg cggcacgtac ttgttgcaga
    12661 acatcttgcc catgtcgctg aagggcagca cggggccgac catggtcttc tcgtcgaaga
    12721 ccatctgacg gtacatgccc caattgatgt cgccggagtc gggctcgcgc gacaccacca
    12781 tgtgccaggt ggccagatag cggccgccgt caccatcgtg caccataggc ccgggcaggt
    12841 cgaacaggtc cacgtcgtcg cccaggatca cgttctgctt gcagggtgcg gacgcgcgac
    12901 tcacctccac cgggtcgatg ggcgtgccgc cggtgcgctc cgtgtacgat cgcccgatgt
    12961 ccggtatggg cgtgtccggg gccagaccca gcgccagcgc cagcttcgag tgagtggcca
    13021 ggggtgcgcc cagagcctcg aacccgggat agtctttgat gttcctgaag tagggggcgg
    13081 gccccttcat ctcgcacacg cgccgcacga cggcgcccat ctcgagatcc cagtcgacct
    13141 cctgctccac ggtcacgagc agtccggcct cgtcgagccc cttgatgaac tcgcgattgt
    13201 ccttgtagta cttgttcgcc atcaacccca tcccttggac gagaccttgt ccaccagacc
    13261 ctgccacttg tcctgggccc gcaagatgaa ctcggccagt tcccgaagca tgccttcgcc
    13321 gccgctcctc cccgtgaccc agtgagagat cgcctgcacc tcggccacac cgttggcggg
    13381 cgatacggcg aggcccactt ccttcatcac gcccatgtcg atgatctcgt cgcctacgaa
    13441 gcagatctcg tcgaagccga tacccatctc gccggccacc tcttcgagct tcgccagctt
    13501 gttcttggcc tggaacacgt tcttcacgcc gatctcggcc gcgcgcaact ccacgatctt
    13561 cgacttgcgc gtcatgaaag cgacgtccac gcccagcatc atgaggcagt tggcgccgaa
    13621 accgtcgtcg tggtggaagg cttggaactt catgccttcg tcgttgtaga ggatcatgcc
    13681 gctggtgagc acaccgtgca cgtcgagcac caccagcttg atcttggccg cccgctcgac
    13741 ggcggcgtcc ctatccttct ccatggccat ctccccctcc tgatgggttc gtgatcgtgt
    13801 ttcgtgcggc gtgccgcggg ccagtcgagc cgcgggtcag tcgagcccca gctcggacca
    13861 gcgcgccttg atcgactcga gcaccgcggg cgacatcttc acttccaccg gcttctcctt
    13921 ccactcgaag ggcacggtgg cgtccaggat gatgcgggag gtgatgaact tgttgcccgg
    13981 gtcgagcgcg gggtcgagcg gcgtggaccg gcccctgttg atgatctgag tgccgcgggc
    14041 cgcatcgtag cgagtggcca gggcccacca gacgcgctgc aggtcgtcgg cttggatgtc
    14101 gtggtcgacg atgatgaggc ccttcaggcc gtaggtgcaa gtgttgctgg cgatgacggc
    14161 ggccgccacc tggttggagt gcccggggta catctgcttc accgagacga tggcccagaa
    14221 gcggccgcag gcctccggcg gcatgtacac ggactcgatg cccttgatgc ccatcttggt
    14281 aagctcggtc caaagcgtgg cgttgcggac gaaggccagc atcatgtggt tgtcgttcac
    14341 ggggcgaccc acggaggcag ccaagaagat gggatcgttc cggtggtaga tgcgctccac
    14401 ctggatggcc ggcttgggca ccgggtggta gagctcctcg gtgtagtagc cggtgtactc
    14461 gccgaacgga ccctcgggac gcaggtcgtc accatcgatg tagccctcga gcacccactc
    14521 ggccgtggcc gggatgggga ggccggtgag gggcgccgtc acgatctcca cgggctcgcc
    14581 gcggagagtg cccaccacgt cgtactcgct ggcgttctcg accatcgcgc tccccgccag
    14641 catcagcagc gggtcgcagc ccacgatcat gcatacgggc atcttctcgc cgcgcttgcg
    14701 atacttctgc aggatgcggt cgccgcgctt gcccttgagg atctgggcgc cgaccgtctt
    14761 cttgtcgagc acctggctgc ggtaggtgcc taggttgatc tcgccggtct cggggtcgcg
    14821 caccaccatg aagcaggcgg tgccgatgta gcggccgccg tcctgctcgt agaacttggg
    14881 cgaggggatc ttgaagatgt cgatgtcgtc gccctcgatg acattctcga agatcggacc
    14941 cgtctccagt tccttggcac ggatgacctc gcccaccgac agcttcatcc attcgtgcga
    15001 gagctcgacc atggtcatgt cggcccgccg cccgaggacg gtggccagcc ggcgggtgtt
    15061 gctgtagaca ccgaagagca ccgactgtga cttgtcgtgc cccttgatgt tctcgaacag
    15121 gagagccggg ccttgcttct cttccacgag cttggcgata tggctcatct cgagatccca
    15181 gtcgacctcg gcggtgaccc gcttcagatc gccctgcttc tcgcattggg caatgaagtc
    15241 gcgaagatcc atgcttcccc ctttttccgc ctgccaccga cgagaccgag cgcctcacgg
    15301 gagtccgcct tgggacggcg tcacgccccg cggtcacccc gtcatcgcac tctatgtgcg
    15361 gaggcgcagt gtgtctaatg cttatttctt gcgtgatgta taaggtgaga tatggttagc
    15421 cactcatgtg acggcgcgcg cacggtggcg gcgtcgcgcg gggcttccca tgccggaggc
    15481 gccggcccag gtgaggtgat ggctacggtc gaagccacgt gcgacgtccc ggcctcggcg
    15541 tcacgccgag gccgggggag gatcacttga tgcgtacccc ggtcaccagc tggctgacga
    15601 tccggtccag cgcatccttc ttctgctcgg tctttccctg ctccgccttc ttcgaccaca
    15661 ccgactccgg acgcacctga aggatgatga cgctatcggg gaacggcagg tcggcgtcga
    15721 tggcgaactc gatgtcgtag ggcttgccgt agtacttgtc gatggctttg ctcacccgga
    15781 cgatctcctt gagttcctcg ggagccacgc aggggcgccc acggtcctcg gcgcacacgt
    15841 ccaccgtctt gatgtcactg ccgccttcgt tgtagacgca ctgcagctcc ttgctgccca
    15901 gagtggtctt gatcgtactc agcgtgacct tgtccaccag gtagttgtca ggcgtgacaa
    15961 gtccgctcac gaccgcttcc ccgagtccgt agctcacatc gactgcgatc ttggagcgat
    16021 cgccgttgag cgggttcaag gtgaacgaga ctccggacac gcgggagttg accatcttcg
    16081 ggatgccaac gccgatgttc agaaggaacc ccagcccctt gttgagccga taggtgatcg
    16141 cctctacgtg ataggcgctc gcccacgtct tcttgatgta ctcgaccagg tccttctcgc
    16201 ctcggatgtt cagataggtc tccatctggc cgggcatgga cacggcgcca ctcgaacgca
    16261 ccgcgaccgg gacgttctgc acaccggccc tctcgcacag ccgctggtag gccccgcagg
    16321 cctcctcgac gatggcggcg ggaaccgtcg cctccgtgat gagcttcacg gcgacgtcgc
    16381 cggcctggct gcacgcttcc agagtcgcct ctcccaggga ttcgagatac gcagcgagct
    16441 cgtccttgat gcctgtctcg atgatgaaga ggtcgttcgc gtgaagcgtc agggcaaaac
    16501 cgggactcgt cctgatcccg cccttgagca tctcgcccaa gttggcgttc ttcttgccga
    16561 cgagaggaaa gtcgctgcct tccagctcct cgtaccagaa caggatcctg ctttccttgt
    16621 cggacatcgg gtctacctcg tctccctgat tcaggcgccg gcgggcaggc ccgccggcgc
    16681 ctcgctcctc ttccgtcagg cgcgttcgac gatagtgacg gtgcccgcat cgccgtcgat
    16741 gcgcaccaga tcgcctgtct tgatgaaggt ggtgccgcga ccagtgccga ccaccgtcgg
    16801 aagcccatac tcgcgacaca cgatggcagc gtggcacata gccccgccga cgtcggtgac
    16861 cgcaccggcg atcttcgtga aggccggggc ccaactcggc gaggtggtgg tggccaccaa
    16921 tatctcaccc tccagcaggc tgcccagctc ttccgcagac ttgatcaccc gcgcgcggcc
    16981 ctccacgacg ccggaggacc caggggaccc cgcgagctcc gtggcatccg cgtcgccgcc
    17041 ggtgcccatg cccttcagcc agttcttcaa gaccgacgtc gtcacacccc agagcacgat
    17101 gctgaatggc tcggtcacga cgtcgggggg cgtaccgagg gccggcgggg gcgaccacgc
    17161 ctggaacttc tccatgacac ccttgcgcca ctcgatctcc ttgggccagt acgacgggcc
    17221 gcggccgatg gtgccggtgg cccagctggt gacatggtcc cacaaggcat cgcggatctc
    17281 tccgcgcttc agcagccaga tgtcctcgcg atccttgaag aagccggcat cggtcaggat
    17341 gtcggacacc tgccggacct tgttccagaa gatgctgtgg aaccagtgct ccacatagaa
    17401 gagatggtct tcgacatatg ggaagacacg ctgagagatg gcgagggtct ggtcgaaggc
    17461 ctcccggtcg gcgtcgttgt cgatcagcgc ccggtactcc tcgacgatgc tcctgctctc
    17521 ctcgatcagc ttctcggtgg ggcgacccac ttccttgccc gccttgatcg cctcgaggtg
    17581 catgacgatg gaggcgaagg gcacgtccag gttgtcgttc caggagtagt ggtggtggta
    17641 ccacccggtg cccgtggaga tgtagaacca gggatagcgg gc
//
LOCUS       DASRMJ010000301         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_536786, whole genome shotgun sequence.
ACCESSION   DASRMJ010000301 DASRMJ010000000
VERSION     DASRMJ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536786"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..306
                     /locus_tag="VFD74_08340"
     CDS             127..306
                     /locus_tag="VFD74_08340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK49594.1"
                     /translation="MVTFPRLAKPGAPAMHEFHVHLRTSIPEGVPMSVLEAMVTGIQV
                     VATDVGGGLGGLTPR"
     gene            303..1586
                     /locus_tag="VFD74_08345"
     CDS             303..1586
                     /locus_tag="VFD74_08345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK49595.1"
                     /translation="MMRGRDLICVSSLDWDAHWGCVHQIMSRLAQANRILYVEEPVTM
                     LAPLRVRRHWLRWRAAVPAVRRASAGVWILTPPPLLPFGNVRPWVNKLNQRVLGFYIR
                     WASRRLGFTDPMLWTYLPTSIDLLDRVASSAVVYHCVDEHSAFPGFVSREVVKGYDDA
                     LTARADLVITTAANLRDSRAHLNPRVHHVPNAADAAHFGAARDPALPLPADVASLPTP
                     RIGVVGIHDERLDVAAIEAIARHDPDWTVLVVGPLRPGDVDEARLRACPNVHVLGARL
                     MSELPAYLKAMDVALIPYKLDELTRNIFPLKIYEYLAAGLPVVSAALPELAGIGNDVL
                     FAKTPAEYPALVVQALAEDSAARRAARVAFAARNTWDDRVEAISHLVEGMLSERASAA
                     APAAEGRAVEGSAGAPPAPPVAEPPADHPSDEDGA"
     gene            1583..>2746
                     /locus_tag="VFD74_08350"
     CDS             1583..>2746
                     /locus_tag="VFD74_08350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZK49596.1"
                     /translation="MRILLPTICAPGEIGGVLTHLEMLSSGLEESGHEPRVLYLGGAL
                     PHGAGKMTIRWPAGVLNHVRQGWGMMYAAMVRGRLLVAVTERELKRAEAAGEPWDVLN
                     AQEVYSIPALRAVADRHGIPLVLTLHGYPFYESVSEGYSRASKVGRSFLMESEIRALR
                     LADAVAAVDTRLHRHALWLAPEVATTTSLLMNFIDTTSYAPSLEGRGESRGAWDVPGD
                     TVVLLCPRRLVKKNGVVHPALALAAMPPHQRARFLLLYAGDGGEREAIERVVRDEGLE
                     HEVRLLGGRDREEMRQLYRSADIVLVPSVHSENVEEATSLSALEAMACGCPVIAGAVG
                     GLAEMIDDGVNGLLVPASDPRALAAAILRLADDLTLRSRLAEAARSYVVRKHSH"
BASE COUNT          387 a          928 c          960 g          471 t
ORIGIN      
        1 gtgtgaatgt agcagacaca gtcgttcgac acccgcggaa ggcgtggctc gcgcggctcc
       61 gcgcgggtcc cgacgccgtg gctcgcgcgg ctccgagtgt ggtcggtagc gctgcgactc
      121 gaacgggtgg taacctttcc ccgcctggcg aagccgggcg cgccagcaat gcacgagttc
      181 cacgtgcacc ttcgaacctc gatcccggag ggtgtcccga tgtcggtgct cgaagccatg
      241 gtgaccggta tccaggtggt ggccactgac gtgggaggcg gactgggggg cctgactcct
      301 cgatgatgcg cggacgcgac ctcatctgtg tctcttctct cgattgggat gcgcactggg
      361 gttgcgtgca ccagatcatg agtcgcctcg cccaggcgaa ccgcatactc tacgtggagg
      421 agccggtgac gatgctggca ccgctccgcg tcaggaggca ctggctgcgt tggcgggccg
      481 cggtcccggc ggtgcgtcgc gcgtcggcgg gagtttggat cctcacccct ccgccgctcc
      541 tgccgttcgg caacgttcgg ccgtgggtca acaagctgaa ccagcgggtc ttgggcttct
      601 acatccggtg ggcgtctcgc cgcctcgggt tcacggaccc aatgctgtgg acctacctcc
      661 ccaccagcat cgatctcctc gaccgcgtgg cctcgtcggc cgtggtctac cactgcgtgg
      721 acgaacactc cgcgttcccg gggttcgtgt cgcgcgaggt cgtgaaaggc tacgacgatg
      781 ccctcaccgc ccgcgccgac ctcgtcataa ccacggccgc caaccttcgc gattcgcgcg
      841 cgcatctgaa cccccgcgtc caccacgtgc ccaacgccgc ggacgcggcc cacttcggtg
      901 cggctcgcga ccccgccctg cctctccccg ccgacgtggc gtcgctgccg actccccgca
      961 tcggtgtggt cgggatacac gacgagcgcc tggacgtggc ggcgatcgag gccatcgccc
     1021 ggcacgaccc ggattggacc gtgctggtgg ttggacccct gcgacctgga gatgtcgacg
     1081 aggcccgcct gcgcgcctgc cccaacgtgc atgttctcgg cgcacggctg atgtcggagt
     1141 tgcccgccta tctgaaggcc atggatgtgg cgctgatccc gtacaagctc gacgaactca
     1201 cccggaacat cttcccgctg aagatctatg agtatctggc ggccggcctt cccgtggtct
     1261 cagcggcgct tcccgagctt gcaggcatcg gcaacgacgt cctgttcgcg aagactcccg
     1321 cggagtatcc ggcgttggtg gtgcaggctc tcgctgagga ttcggccgct aggcgcgccg
     1381 cccgcgtggc gttcgccgcg cgcaacacct gggacgaccg cgtggaagcc atctcgcact
     1441 tggtggaagg catgttgagc gagcgggcgt cggcggcggc gccggcggct gaggggcgcg
     1501 cggtcgaggg gtctgcgggc gcgccgcccg cgccgcccgt ggccgagccg cccgcagacc
     1561 acccctctga cgaggacggg gcatgaggat cctgcttccg accatctgcg ctcccggcga
     1621 gatcgggggg gtgctcacgc atctcgagat gctctcgagc ggcttggagg agagcggcca
     1681 cgagccgcgc gttctctacc tcggcggggc cttgccgcac ggggcgggca agatgactat
     1741 ccggtggccc gccggagtgc tcaaccacgt gcgccagggc tgggggatga tgtacgcggc
     1801 gatggttcgc ggacgcctcc tcgtggccgt gacggaacgg gaactgaaga gggctgaggc
     1861 cgccggcgag ccttgggacg ttctgaacgc ccaagaggtc tactccatcc ccgccctgcg
     1921 cgccgtcgcc gaccgccatg gcatcccgct ggttctgacc ctccacggtt accccttcta
     1981 cgagtcggtg tcggaagggt actcccgcgc gtccaaagtg ggacggtcgt tccttatgga
     2041 gtcggagatc cgcgcgttgc ggttggccga tgccgtggcg gcggtcgaca ccaggctgca
     2101 tcggcacgcg ttgtggcttg cgccggaagt ggcgaccacg accagcctcc tgatgaactt
     2161 catcgacacg acgtcgtacg ctccgagcct cgaaggccgt ggcgagtcga gaggagcgtg
     2221 ggacgtgcct ggcgacacgg tggtcctgct ctgtccgcgc cgcttggtga agaagaacgg
     2281 ggttgtgcat cctgctctgg ccctggcggc catgcctccg catcagcgcg cgcgcttcct
     2341 cctgctctac gcgggagacg gtggcgaacg ggaagcgatc gaacgcgtgg ttcgcgacga
     2401 gggactggag catgaggtgc ggcttctcgg tggccgcgac cgcgaggaga tgcggcagct
     2461 gtaccggtcg gccgacatcg tgctcgtgcc gtcggtgcac tctgagaacg tggaggaagc
     2521 cacctcgctc tcggccctgg aagccatggc ctgcgggtgc ccggtgatcg cgggggcggt
     2581 gggaggtctg gccgagatga tcgacgacgg cgtgaacggg ctgctcgtgc ccgcctccga
     2641 tccccgcgcg ctggccgccg cgatcctgcg attggccgac gacctcactc tacgctcgcg
     2701 gctggccgaa gcggcccggt cgtacgtcgt gcggaagcac tcacac
//
LOCUS       DASRMJ010000302         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_536930, whole genome shotgun sequence.
ACCESSION   DASRMJ010000302 DASRMJ010000000
VERSION     DASRMJ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536930"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFD74_08355"
     CDS             complement(<1..773)
                     /locus_tag="VFD74_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A domain-containing protein"
                     /protein_id="HZK49597.1"
                     /translation="MALFLLTLVPAAMAMPDADGGEGAAKSTVGRIDNRPDLLGTKQA
                     ALKQSALEKKLHGKKSGTTVQVADGQFVELGRTGTDAIWTVLGQFADMPHNEIAQPNR
                     TYNNSTIWEPDFSRDYFLDLLFDESEGANSMSNWFIEQSSGRYSVTGDVTDWVTVPGS
                     AAGYDDDLILPSGDNAVWQFLTDSVDGWYASQLAAHKTRKQIDKYLSQFDTWDRYDFD
                     GDGNFNEPDGYIDHFQSLHAGEGNEAGGGVLGDSAIWSH"
     gene            complement(1131..1203)
                     /locus_tag="VFD74_08360"
     tRNA            complement(1131..1203)
                     /locus_tag="VFD74_08360"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Thr,seq:cgt)
     gene            complement(1338..2492)
                     /locus_tag="VFD74_08365"
     CDS             complement(1338..2492)
                     /locus_tag="VFD74_08365"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HZK49598.1"
                     /translation="MADVVGIVFQSGGKVYYFDPASLELAHGEQVVVQTMRGTEIGEV
                     VDGPREVPDGELPAPLKRVVRKANIQDVETVQSNIALRAEAMTVCRDLIRKHGLEMKL
                     VDAEIVFGGGKIIFSFSAEERIDFRALVSDLAKVLKMRIELRQIGAREETRILGGLGP
                     CGRDLCCSLFQGDQDPVSIRMAKEQNLPLNPMKISGLCGRLMCCLKYEQEQYVCFRKE
                     VPNRGTKVQTESGEGVVVGFQLTKESLTLRMPDGSLAEVPLSRCGCGGAPCGDAPCGG
                     AAESEAAAGGTVEGGSPRGRREGNGQPPADPARAQRTAAAPPEDAGRRRSGTVVTSGD
                     VADDAGEPESGGAPAAGEESEGRRSPSRSRRRRRRSRKSSGGGQGGQQGS"
     gene            complement(2492..3610)
                     /gene="holB"
                     /locus_tag="VFD74_08370"
     CDS             complement(2492..3610)
                     /gene="holB"
                     /locus_tag="VFD74_08370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZK49599.1"
                     /translation="MPLFADILGQTLALTSLRQALAHGASHAYLFAGPAGVGKSDTAL
                     AFAAGLACPDRGCGACETCRRVLEGLHPDVEIVSPEGAFITIDQIREINREVVMRPFE
                     ASVRVYVLLDAHTLKNEAANALLKTLEEPPPHAHFILVTDSPQALPQTVVSRCRRVLF
                     TSVASPVLAAHLVAAYGLSEVEALAFARVAHGSPVHARALATDPGARSQRERLLGWAR
                     SVGEAGAFDAHVMLTEIMDSVERRADERVKLLEDDTAARLQWAPDARTRARIEKLHED
                     RVKRGRRRAVTEGLDEVTATFATWHRDLAAVAVGAEEAVFNYDFLFELRDRAFPGLVD
                     QYMEVVEAVKRTRERFRYNVDARCALEEMIFSIREALQ"
BASE COUNT          626 a         1296 c         1237 g          670 t
ORIGIN      
        1 ctgtgactcc agatcgcgct gtcgccgagc acaccgccgc cggcttcgtt gccttctccg
       61 gcgtggagcg actggaagtg gtcgatgtag ccgtctggct cgttgaaatt gccgtcacca
      121 tcgaagtcgt agcggtccca cgtatcgaac tggctgaggt acttgtcaat ctgcttacgc
      181 gtcttgtgcg ctgccagctg cgaggcgtac cagccgtcga ccgagtctgt caggaattgc
      241 cacacggcgt tgtcaccgct cggcaggatg aggtcatcgt catagccggc ggcgctgccc
      301 ggaacagtga cccagtcggt cacgtcgcct gtgacggaat atcggccgga cgactgctcg
      361 ataaaccagt tactcatcga gttggcgcct tcggattcgt cgaacagcag gtctaagaag
      421 tagtctcgac tgaagtccgg ctcccagatc gtgctgttgt tgtacgtgcg attgggctgc
      481 gcgatttcgt tgtgcggcat gtcggcgaac tggccgagca ccgtccagat ggcgtccgta
      541 cccgtgcggc cgagctcgac gaactggccg tcggcgactt gcaccgtagt cccggatttc
      601 ttgccgtgga gcttcttctc gagcgcagac tgcttcaacg ccgcctgttt tgtacccagc
      661 aggtccggcc gattgtcgat cctccccacg gtgctcttgg cggcgccctc gccaccgtcg
      721 gcatcaggca tcgccatcgc cgccggcacc agtgtcagca gaaacaacgc cataatcaga
      781 aagactcgta gaatcttgcc cctcatcccc ttcttcccct ctccatatct ctcagtctgg
      841 accgattcat ctctcttggg agcgaacaga cgcggcgata ggtaccccca gccgatagtc
      901 gtctgcccaa gtactacact gtgacgacaa gcgccacagt aggaagcgcg cgtcacattc
      961 tcaagagggg agctctcggt gcccaaagcc gcgagctgaa cggaggtggt ctacctcaag
     1021 cttggcgatc ggcactgtgg ccggggacgt cgagagcgtg tgcaagcgag ggcggttcac
     1081 ttcttgctgg ggcgctcgtt catgagcggc ttccgcgcca gaacaaggaa agccgacgtg
     1141 gggagtcgaa ccccagacct gctcattacg agtgagctgc tctaccaact gagctacgtc
     1201 ggcccggcgg gcgcgggcga cacccagcga ggtgctgagg gcccgtccgc gggggattag
     1261 tttagtgggt cgccccaagg ttggcaaggt gacgaggcgg gcgggcgtcg acccgctggg
     1321 aacgcctccc catcacccta cgagccttgc tggcctccct ggccgccgcc cgatgacttc
     1381 ctggagcggc ggcggcgacg gcgcgacctg cttgggctcc gcctcccctc gctctcctcg
     1441 cctgccgcgg gggctccacc ggactcgggc tccccggcgt cgtcggccac gtcaccagaa
     1501 gtcacgacgg ttccggagcg gcggcggccg gcgtcttcgg gcggcgccgc ggctgtccgt
     1561 tgcgcgcgtg ccggatccgc ggggggctgt ccgttgcctt ctcttcttcc gcgaggactc
     1621 ccgccctcca ccgtgccgcc ggcggccgcc tcactttcgg cagcacctcc gcacggagcg
     1681 tctccacagg gggcaccgcc gcagccacag cgactcaacg gcacctccgc caacgatccg
     1741 tcgggcatgc gaagggtgag cgactccttg gtcagctgga agccgaccac gacgccttcc
     1801 cctgactcgg tctgcacctt cgtgccccga ttcggcactt ccttgcggaa gcacacgtac
     1861 tgctcctgtt cgtacttgag gcagcacatc aaccggccgc acagtccgga gatcttcatg
     1921 gggttgagcg gaaggttctg ttccttggcc atgcggatgg acaccgggtc ctggtccccc
     1981 tggaagaggg agcagcagag gtcacgcccg cacgggccga gaccgccgag gatacgggtc
     2041 tcttcccggg cgccgatctg acgcagctct atgcgcatct tcaggacctt cgcgaggtcg
     2101 gagaccagcg cgcggaagtc gatcctctcc tccgccgaga agctgaagat gatcttgcct
     2161 ccgccgaata cgatctccgc atcgacgagc ttcatctcga gaccgtgctt gcggatgagg
     2221 tcgcgacaca cggtcatggc ttcggcacgc agggcgatgt tgctctgcac cgtctccacg
     2281 tcctggatgt tggccttgcg cacaacacgc ttcaatggcg cgggaagctc gccgtcgggg
     2341 acctcccgag ggccgtcgac gacttcgcct atctcggttc cacgcatggt ctgcactacg
     2401 acctgctcac cgtgtgcgag ctccaaggag gcggggtcga agtagtagac cttgccgccg
     2461 ctctggaaca cgataccgac cacgtcggcc attattggag ggcctcccgg atagagaaga
     2521 tcatctcttc aagtgcgcaa cgcgcgtcga cattgtacct gaatcgctcc ctggtcctct
     2581 tgacggcctc cacgacctcc atgtactggt cgaccaagcc ggggaaggcc cggtctcgca
     2641 gctcgaagag gaagtcgtag ttgaacacgg cctcctcggc gccgaccgcc actgcggcga
     2701 ggtcccggtg ccaggtggca aaggtggccg tcacctcgtc gagaccctcg gtgaccgccc
     2761 gccgacgccc gcgcttgacc cgatcctcgt gaagcttctc gatgcgcgcg cgggtgcggg
     2821 cgtcgggggc ccactgcaac cgcgccgcgg tgtcgtcctc gaggagtttg acgcgttcgt
     2881 ccgcgcgccg ctccaccgag tccatgatct cggtgagcat cacgtgcgcg tcgaaggccc
     2941 cggcctcgcc gacgctccgc gcccacccca gcaacctctc tcgctggctg cgcgcgcccg
     3001 ggtccgtggc gagcgcgcgg gcgtggaccg gactgccgtg ggccacgcgc gcgaaggcca
     3061 gagcttcgac ctcgctcagg ccgtaggcgg cgacgaggtg agccgccagc accggcgacg
     3121 ccaccgacgt aaacagcacg cgccggcatc tggacaccac cgtctgtggc aaagcttggg
     3181 gtgagtcggt caccaggatg aagtgcgcgt ggggcggtgg ctcctccaac gtcttcagga
     3241 gggcgttcgc tgcttcgttc ttcagagtgt gagcgtccag aagcacatag acgcgcaccg
     3301 aggcctcgaa gggccgcatg accacctcgc ggttgatctc tcggatctgg tcgatggtga
     3361 tgaaggcgcc ctcaggcgaa acgatctcca catcggggtg gagcccctcg aggacccgcc
     3421 ggcaagtctc gcacgcaccg caccctcgat cgggacaggc cagcccggcg gcgaaggcca
     3481 gcgcggtgtc gctcttgcca actcctgcag gacccgcgaa gagatacgcg tgcgaggctc
     3541 catgggcgag cgcctgccgc agtgaggtga gggcgagcgt ctggcccagg atgtctgcga
     3601 agagaggcac tcgacaagtg tagcaggggg agcggcgacg acggcgggcc gagggggccg
     3661 gggaccccgg tgcactgaag tgggccgggc tcggcgggcc agaggggggc ctggcccggc
     3721 caggcgcacc ggctcctggg ctcgcccctt aggaaccccg cgagaagagt gccagctcct
     3781 tgtcgacgat cgcccgcacc cgcgactgca cctcggtcac ggcgcccac
//
LOCUS       DASRMJ010000303         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_171106, whole genome shotgun sequence.
ACCESSION   DASRMJ010000303 DASRMJ010000000
VERSION     DASRMJ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171106"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VFD74_08375"
     CDS             complement(<1..129)
                     /locus_tag="VFD74_08375"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZK49600.1"
                     /translation="MSGKRLYRSRSEKMLGGVCGGLADYLDFDVTLIRILWVLATLL"
     gene            complement(193..462)
                     /locus_tag="VFD74_08380"
     CDS             complement(193..462)
                     /locus_tag="VFD74_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49601.1"
                     /translation="MSVFILEEVNEMVEIHYCENCDEHAAEVVLSGPGGESTYLCTSP
                     ECMMAAGICPSCHVDLEVHDSHTGCKLYSCPQCDYERTYSELGQA"
     gene            complement(502..693)
                     /locus_tag="VFD74_08385"
     CDS             complement(502..693)
                     /locus_tag="VFD74_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49602.1"
                     /translation="MTDTLKTCIECGRPLADRVEEEERTGIGASRSTGECDTYRVWSC
                     VNTVCVLHGSDQYREQIGQ"
     gene            893..1474
                     /gene="dcd"
                     /locus_tag="VFD74_08390"
     CDS             893..1474
                     /gene="dcd"
                     /locus_tag="VFD74_08390"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007316415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZK49603.1"
                     /translation="MILSDSDILAALEAGHIALDPFDRSLLQPASVDIRVDARFRIFS
                     NHLYTCIDPRLCQDDLTSLVQVDAEGSFVLHPGEFVLGSTIERVTLSPAYVARLEGKS
                     SLGRLGLMCHATAGWIDPGFSGHITLELSNVANLPIRVYPGMPIGQLSFARLSSPAAH
                     PYGADDMDSKYQGQDGPTESRMWKWFRPPASES"
     gene            1480..2214
                     /locus_tag="VFD74_08395"
     CDS             1480..2214
                     /locus_tag="VFD74_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HZK49604.1"
                     /translation="MFDSAHLRAGVERIARRLGAYRVGVASLEDEAVSAAVREQGDER
                     LAGFSRAVSIAFRLQDAIVGRLPEEHLTPLVAKTYRFHIYTVVNAHLDQVATLVAGVL
                     QDAGHAALSVPTSLSVDAEGFRGPLSHKLAARMAGLGWIGRSCLLVTPEVGPRVRLVT
                     VLTDAPLAADVPLSRDCGACTLCVEACPAGAFTGRPFTPSEPIEARMDVRACARYRAS
                     AKDQSGVDICGVCVAVCPHGRRGKKG"
     gene            complement(2211..3353)
                     /locus_tag="VFD74_08400"
     CDS             complement(2211..3353)
                     /locus_tag="VFD74_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK49605.1"
                     /translation="MKLRTRLFLGFLVVIAVVVVVVAVLVRGVAVGAVSSHMGQGMGG
                     MMGGGMMADVESAVARGVTDALIVAALVAVTVAAVASYLLSGVITATIGEMAGAARRI
                     AAGEYAQRVVHPAKDEIGEFADAFNEMASRLDQTEQVRRELLATISHELRTPLTSIQG
                     YMEGLLDGVIPEEPATYQLVHREAARLSRLVADIENFSRIEAGVERIAPAELDVGVIL
                     NGVADRVRPQFEQKPLTVTVIVEPATPHVRANEDRLLQVLLNVVGNSFKYTPPGGNVN
                     LRAGPAPREWAEELRPRPGSGSAAGYVLFQIEDTGIGIPAVDLPHVFERFYRVDKSRS
                     AAGGGLGIGLAVARSLVAQMGGAMRMESAPGEGTTVSFTLPAAGGS"
     gene            complement(3350..4048)
                     /locus_tag="VFD74_08405"
     CDS             complement(3350..4048)
                     /locus_tag="VFD74_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006723535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK49606.1"
                     /translation="MGKPIRILVVDDDDNVRRLVAAYLEREGYAVTQAADGNSAIKAV
                     ETASPDLLILDLMLPGPGGLQVARRLKDSVGVPVIMLTARGEEDDVLRGFEAGADDYL
                     VKPFSPKVLVARVRAVLRRAGQGADEDTGPVVRGPLVIDERSREASVDGVVAELTAIE
                     FDLLRALADHPGWVYSREQLLESVWGYDHLGDSRVVDVHVANLRRKIGDDPTQPRFIR
                     TVRGVGYKFQVPGE"
     gene            complement(4085..>4271)
                     /locus_tag="VFD74_08410"
     CDS             complement(4085..>4271)
                     /locus_tag="VFD74_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK49607.1"
                     /translation="TTYKTVFDGARQVVNYFWRVLDVQPIGDLLCPGVDAKGEILKFP
                     SAREIAEDIGRRLAEPR"
BASE COUNT          707 a         1541 c         1314 g          709 t
ORIGIN      
        1 cagcaaggtc gccagcaccc agaggatccg gatgagggtg acgtcgaagt ccaagtagtc
       61 cgccagccca ccgcagaccc cacccagcat cttctccgaa cgagacctgt atagccgctt
      121 gccgctcatc gatgcttcct cctcacgacg gaactttcgg actcttgccg gaaccccatg
      181 ccgacgttga cgtcacgcct ggccgagttc cgagtaagtg cgctcgtagt cgcactgcgg
      241 gcacgagtag agcttgcagc ccgtatggga atcgtgcact tccaggtcca cgtggcaact
      301 cgggcagatg ccggcggcca tcatgcactc gggcgacgtg cacagatagg tggattcgcc
      361 accgggtccg ctgagcacca cttccgccgc atgctcgtcg cagttctcgc agtagtgtat
      421 ctcgaccatc tcgttgacct cctccaggat gaacaccgac actgcggccc ggcgtcgact
      481 gccccgccga ggccgctcgg tctactgacc gatctgttcc cgatactgat cactgccgtg
      541 cagcacgcac accgtgttca cgcaggacca cacgcggtag gtgtcgcact cgcccgtcga
      601 ccggctcgcg ccgatccccg tgcgctcttc ttcctccact cgatctgcca agggccgacc
      661 gcactcgata caggtcttga gggtatctgt caccgcatgc tccattcgct ctagacgaac
      721 cacgacaaac agtctggccc acgcggagcg gccgatgtag aatcagtggc cggccccgac
      781 gtcaccggca cccacgccgt gtcggacacc gaagtcgcga gcacgaacgc cgcgcaaagc
      841 gccggcgcca ccggcccgaa ggctaccaga ccgcgaacga aggcagaagc cgctgattct
      901 ctcagactcg gacatacttg ccgcgctgga agccggtcac atcgcccttg acccgttcga
      961 ccggagcctc ctgcaaccgg ccagcgtcga catccgcgta gatgcccgct tcaggatctt
     1021 ctctaaccac ctctacacgt gcatcgaccc tcggctctgc caggacgacc tcacctcgct
     1081 cgttcaggtg gatgccgagg gctcgttcgt gcttcatccc ggggagttcg tcctggggtc
     1141 gaccatagag cgggtcacac tgagccccgc atatgtcgcc aggctggagg gcaagagctc
     1201 tctcggccgt ctcggcctca tgtgccacgc caccgcaggt tggatcgatc ccggcttctc
     1261 ggggcacatc accctggaac tctcgaacgt ggccaacctg cccatccgtg tttacccggg
     1321 aatgcccatc ggccagctct cgttcgcgcg cctctcgtcg cctgccgccc atccctacgg
     1381 cgccgacgac atggacagca agtaccaagg acaggacgga cccacggaat cgcgcatgtg
     1441 gaagtggttc cgcccaccgg cctcggaatc gtgaaccccg tgtttgacag cgcgcacctc
     1501 cgtgccggcg tggagcgcat cgcccggcgc ctgggcgcct atcgcgtagg ggtggcgtca
     1561 ctcgaggacg aggccgtctc ggcggcggtg cgcgaacagg gagatgagcg gctggcggga
     1621 ttctcgcgcg ccgtttccat cgccttccgc ctgcaggacg cgatcgtggg ccgactgccc
     1681 gaagagcacc tcacccctct ggtggccaag acctacaggt tccacatcta caccgtggtc
     1741 aatgcgcatc tcgaccaggt ggcgacgctg gtggccggcg tcctgcagga cgccggccac
     1801 gccgccttgt ccgtgcccac gtcgctctcc gtggacgccg agggcttccg cggtccgctc
     1861 tcgcacaagc tggcggcccg catggcgggc ctcggttgga ttgggagaag ctgcctgctg
     1921 gtcacgcccg aggtcggccc gcgggtgcgg ctcgtcacgg tgctcaccga tgccccgctc
     1981 gcggcggacg tccctctctc acgcgactgc ggagcctgca cgctctgcgt ggaagcctgt
     2041 cccgcagggg ccttcaccgg acgccccttc actccgtcgg agcccatcga ggcccgcatg
     2101 gacgtgcgcg cctgcgcgcg ataccgcgct tcggccaagg accagagcgg cgtggacatc
     2161 tgcggcgtct gcgtggcggt gtgccctcac ggtcgccgag ggaagaaggg ctagctcccg
     2221 cccgccgcag ggagcgtgaa ggagacggtg gttccttctc cgggtgcgct ctccatcctc
     2281 atcgccccac ccatctgcgc aaccagactc cgcgcgacgg ccaagccgat ccccagaccg
     2341 ccccccgccg ccgacctcga tttgtcgacg cggtagaacc tctcgaacac gtggggaagg
     2401 tcgacggccg ggatcccgat gccggtgtcc tctatctgga agagcacgta gccagcggct
     2461 gagcctgagc ccgggcgagg tcgcagctcc tccgcccact cacgcggagc gggcccggcg
     2521 cgcaagttga cattcccgcc aggcggagtg tacttgaacg aattccccac gacgttcagc
     2581 agcacctgca gcagccggtc ctcgttggct cggacgtgtg gcgtcgccgg ttccacgatc
     2641 accgtcacgg tcagcggctt ctgctcgaac tgcgggcgca cgcgatcggc cacgccgttc
     2701 aggatgactc ccacgtcgag ctcggcggga gcgatgcgct ccaccccggc ttcgatccga
     2761 gagaagttct cgatatcggc caccaagcga gacaagcgcg ccgcttcgcg atgcaccaac
     2821 tggtaggtcg cgggctcctc ggggatcacg ccgtcgagca ggccctccat gtagccctgg
     2881 atgctcgtca ggggcgtgcg cagttcgtgc gagatggtag cgaggagttc tcgccgcacc
     2941 tgctcggtct ggtcgaggcg cgaggccatc tcgttgaagg catcggcgaa ctcgccgatc
     3001 tcgtcctttg ccggatgaac cactctctga gcgtactcgc cggcggcgat cctgcgagcg
     3061 gcgcctgcca tctcaccgat ggtcgcggtg atgacgccgg agaggaggta gctcgcgacg
     3121 gcggccaccg tgaccgcgac caacgccgcg acgatcagag catcggtcac ccctcgtgcc
     3181 acggccgact ccacgtcggc catcatccca ccgcccatca tcccgcccat gccttggccc
     3241 atatgggagg agacagcccc cacggcgacg ccacgcacca ggacggcgac cacgaccacg
     3301 accaccgcga tcacaaccag gaaccccagg aacagtcttg tgcgcagctt cattctcccg
     3361 gcacctggaa cttgtagcct acgccgcgca ccgtgcggat gaagcgcggc tgggtcggat
     3421 cgtcgccgat cttcctgcgc aggttggcca cgtgcacatc gacgactcgg gagtcgccca
     3481 ggtggtcgta gccccagacg ctctcgagga gctgctcccg tgagtagacc catccggggt
     3541 ggtcggcgag ggcgcgcagg aggtcgaact cgatggcagt gagttccgcc acgaccccgt
     3601 cgacactcgc ctcacggctg cgttcgtcga tgaccagggg gccccgcacc acaggcccgg
     3661 tgtcctcatc tgctccctgc ccggcccgtc gcagcaccgc gcgaacgcgc gccaccagga
     3721 ccttcgggct gaacggcttc accaggtagt cgtcggcgcc ggcctcgaac ccccgcagga
     3781 cgtcgtcctc ttctccccgc gcggtcagca tgatcaccgg cacgcccacg gagtccttca
     3841 gccgccgagc cacttggagt ccgcccgggc cgggcaacat cagatcgagg atcagcaggt
     3901 cgggcgaggc cgtctcgacc gccttgatgg ccgagttccc gtcggccgcc tgggtgacgg
     3961 catacccctc gcgttccaag taggcggcca ccaggcgacg tacgttgtcg tcatcgtcga
     4021 cgaccaagat gcgtatcggt ttacccatga cgcctccgag gccgcggtgg cagcccttac
     4081 tggttcaccg gggctccgcc aggcgacggc cgatgtcctc ggctatctct cttgcggagg
     4141 ggaacttgag tatctcgccc ttggcatcca ccccggggca cagaagatcg ccgatcggct
     4201 gcacatcgag cacacgccag aagtagttca cgacttggcg ggcgccgtcg aagaccgtct
     4261 tgtaggtggt g
//
LOCUS       DASRMJ010000304         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_171293, whole genome shotgun sequence.
ACCESSION   DASRMJ010000304 DASRMJ010000000
VERSION     DASRMJ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171293"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFD74_08415"
     CDS             <1..183
                     /locus_tag="VFD74_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZK49608.1"
                     /translation="LHDLRHSYATLALSAGVHAKVISERLGHASIAITLDTYSHAVCS
                     STEKLTTSIVEKLTTP"
     gene            complement(180..953)
                     /gene="istB"
                     /locus_tag="VFD74_08420"
     CDS             complement(180..953)
                     /gene="istB"
                     /locus_tag="VFD74_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZK49609.1"
                     /translation="MNADITTAQIRLYARELKMPGLSGAFEEVARDAVTAGRGHLESL
                     AACLAAEVESRAEHRLAAGIKAARFPALKTFETFDFSLLPSLEKGRVLGLGSGAFTKE
                     RENVVCLGTSGTGKTHVATAIGLSAVYAGARVRFTTAVALSQELLAAADEHRLPRYLK
                     TWRSADLVIVDELGYLPLGPGAPLLFQFFAERYESGSVLVTTNLEFSRWTEVFGDATL
                     TAALLDRLTHHCHVLVFEGESYRFRESAGRLTKPRHKTS"
     gene            complement(950..2398)
                     /gene="istA"
                     /locus_tag="VFD74_08425"
     CDS             complement(950..2398)
                     /gene="istA"
                     /locus_tag="VFD74_08425"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZK49610.1"
                     /translation="MVDVEFIKKRHEAGWSIRKIARQLEVSRQTVRKVLEAPAEPPTY
                     TSVRPRPSPVMDPFIGFVAQWLRDDETAPRKQRHTAKRAYDRLVDEHGFTGAEVTVRQ
                     AVARLRGRRVEAYVPLEAPWGRIAQADFGGAVVTILGERTEVALFCMRAKASKVPFVV
                     ALPSERLEALLAGHVEAFKFFGGVFSEVWYDNPKTAVTKILSGPERIEHERLSQLRAH
                     YLFDSSFCTPARGNEKGSVENLVGYVRRNALVPHTRSFSSLEALNAHLRAWCERERSR
                     HAAAWEVESAALSGLPKHPFSPSVSRPVVVNKTSLVTVDRVRYSVPVTHVGRRLRSEC
                     FVERVEVFNGSTCVAVHMRSYVRGATVLHLGHYLDAFERKPRAALSCAALSSADPVFT
                     AARDLALRTQEGHRTFAAVLLLAREFGLVRLADALREAMATGAVTPERVRQLALNAAH
                     RVPRPVEVPASLQIPLPSADLTCYDELAVCAS"
BASE COUNT          484 a          846 c          856 g          417 t
ORIGIN      
        1 cttcatgatc tccggcacag ctacgccacc ttggctctct ctgccggtgt tcacgcgaag
       61 gtgatctccg aacgactcgg gcacgccagc atcgccatca ccttggacac ctactcgcac
      121 gccgtgtgtt cgtcaaccga aaagttgacc acctcgatag tcgaaaagtt gaccacccct
      181 tagcttgtct tgtggcgagg tttcgtgagg cgcccggcgc tctcgcggaa ccggtaggac
      241 tctccttcga acacgagtac gtgacagtgg tgggtgagac ggtcgagcag tgcggcggtg
      301 agcgtcgcgt cgccgaagac ctcggtccaa cgactgaact ccaggttggt cgtcacgagc
      361 accgagccgg actcgtagcg ctcggcgaag aactggaaca gcaatggcgc tccgggacca
      421 agcgggagat agccgagttc atcaacgatc acaaggtccg cgcttctcca ggttttgagg
      481 tagcggggga gccggtgctc gtcggctgct gctaagagtt cctgcgacaa cgccaccgcc
      541 gtggtgaacc tgactctggc tcccgcatag accgccgaga ggccgatcgc cgtggccacg
      601 tgtgtcttgc cggtccccga ggtgccgagg cacacgacgt tctcgcgctc cttcgtgaag
      661 gcgcccgacc ccagaccaag gacacgaccc ttctcgaggg acggcagtag cgagaagtcg
      721 aaggtctcga aggtcttgag ggccgggaaa cgggcggcct tgatccccgc cgccagccga
      781 tgctcggccc gcgactcgac ctcggcagcg aggcaggccg ccagcgactc gagatggccc
      841 ctgcccgctg tgaccgcgtc acgggcgacc tcctcgaagg cacctgagag cccgggcatc
      901 ttgagctcgc gggcatagag ccggatctgg gccgtggtga tgtcggcgtt catgaggcac
      961 acaccgccag ctcgtcgtag caggtgagat ccgcagaggg cagcgggatc tgaagcgagg
     1021 cgggcacctc caccggacgg gggacgcgat gggcggcgtt caaggcgagc tgacgcacgc
     1081 gctccggcgt aacggctccc gtggccatcg cctcccgtag cgcgtcggcg agccgcacga
     1141 ggccgaactc gcgggcgagc aaaaggacgg ccgcgaaggt gcgatgcccc tcttgggtgc
     1201 ggagcgccag gtcgcgcgcg gcggtgaaga ccgggtcggc acttgaaagc gccgcacacg
     1261 acagggccgc ccggggcttg cgctcgaacg cgtcgaggta gtggcccagg tgaagcaccg
     1321 tggcacccct cacataactg cgcatgtgga ccgcgacgca ggtggatccg ttaaagacct
     1381 ccacgcgctc gacgaagcat tcgctgcgaa gcctgcgccc cacatgggtc accgggaccg
     1441 agtagcgcac ccggtcgacg gtcacgagcg aggtcttgtt caccacgaca gggcgactca
     1501 ccgagggcga gaacggatgc ttgggtagtc ccgagagcgc ggcggactcg acctcccagg
     1561 ccgcggcgtg acgcgagcgc tcccgctcgc accacgcccg caggtgcgcg ttcagggctt
     1621 caagcgacga aaaggagcgg gtgtgcggca ccagtgcgtt ccggcgcacg tacccgacca
     1681 ggttctcgac cgatcccttc tcattgcccc gcgccggcgt gcagaaactg gagtcgaaca
     1741 ggtagtgggc gcgcagttgg ctcaagcgtt cgtgctcgat gcgctcggga ccggacagga
     1801 tcttggtcac ggcggtcttc ggattgtcat accagacctc cgagaacacg cccccgaaaa
     1861 acttgaaggc ctcgacatga ccggcgagca gggcctcgag cctctccgag ggcagggcga
     1921 ccacgaacgg caccttggag gctttggcgc gcatgcagaa gagcgcgacc tcggtccgct
     1981 cacccaagat cgtgaccacc gcgcccccga agtcggcctg ggcgatgcgc ccccacggtg
     2041 cctccagggg cacgtacgcc tcgacgcgcc tgccccgtag tctggcgacg gcctgacgca
     2101 cggtgacctc cgcaccggtg aatccatgct cgtcgacgag gcggtcatac gcccgcttgg
     2161 cggtgtggcg ctgtttgcgg ggggcggttt cgtcgtccct aagccactgc gcgacgaagc
     2221 cgatgaacgg atccatcacc ggggacgggc gtggtctcac cgatgtatag gtgggaggct
     2281 cggcgggagc ctcgaggacc ttgcgcaccg tctgccggga cacttcgagt tggcgggcga
     2341 tcttgcggat cgaccagccg gcctcgtgac gcttctttat gaactccaca tcgaccatct
     2401 tcagcacatg cccttcccct gtccgcaaaa gcacccgaca acggggctga cggtacagga
     2461 ctcgtccttg aaggtggtca acttttcgag tatcgaacgg cctccaagtg gtcaactatc
     2521 agggtatcga acacaaaact ggagggacgg atgcgatgaa cgagccaaag cagatcgatc
     2581 cactttattg gctcgtgaac acc
//
LOCUS       DASRMJ010000305         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_66860, whole genome shotgun sequence.
ACCESSION   DASRMJ010000305 DASRMJ010000000
VERSION     DASRMJ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66860"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFD74_08430"
     CDS             <1..835
                     /locus_tag="VFD74_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK49611.1"
                     /translation="RCTFCAMYKDKRFRVRPLEDVLEDLDLARATFAHVPAIFLADGN
                     TIALPTDHLLAILAHAHTLFPELEHVGLYGGARYLARDPGPDDAGRPGLAAGKSVTEL
                     AELRGAGLSAVYLGLESGDAEVLRNVKKGVTPDDMVKAGRRLKEAGLPVSLYILVGLG
                     GLHRYREHALGTAHVINAIGPDIVRPRTLYIQEGTPLWHQRRRGEFVETGPREALLET
                     RLLLENITVPLTLLSDHITNYLPLHGRLPEDRDRLIDAIDLALANDDLDSLRPTHFDH
                     L"
     gene            1143..1925
                     /locus_tag="VFD74_08435"
     CDS             1143..1925
                     /locus_tag="VFD74_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZK49612.1"
                     /translation="MLAIASIGLVAGFLSGLLGIGGGIIVTPLLLYMPQLVGASTLSI
                     RTVAGLTIVQGLLASLVGAISHRRFKFSSGRLAAYMGATIFVAAFLGGAGSKLVSNTV
                     LLVLFLALACAGGILILLPTKNEEEYPAGAALDFNPARAVAVAAGVGLLGGMVGQGGS
                     FILIPLMTWFVKVPTRIAIGSNLAVVLFSSAAGFLGKAFTGQIEWLLAVPLVLTVIPS
                     AALGSRVSRRLSVLTLRRTLAVVIGVAVVRIAVSLLSGEVAG"
     gene            complement(2030..2584)
                     /locus_tag="VFD74_08440"
     CDS             complement(2030..2584)
                     /locus_tag="VFD74_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK49613.1"
                     /translation="MERQAALKLVRERVGNENLVNHMLAAEAIMVALANRLGGDPARW
                     GLTGLLHDLDAEQTAEMMTVHATRTVEWLRAEGLDDEEILDAILAHNPANGSTITTTM
                     ARALFAADPLTGLVTAAALIRPEKKLELVQLKSLKKRFKEPSFARGARREDIATCEEL
                     GIPLEEFLALGLDAMKAIAGDLGL"
     gene            complement(2705..>3131)
                     /locus_tag="VFD74_08445"
     CDS             complement(2705..>3131)
                     /locus_tag="VFD74_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA+ family ATPase"
                     /protein_id="HZK49614.1"
                     /translation="IVSVVDAASPGLVVKPEVARRQIDVESAKPDPPPSAEDDGSRGS
                     APGAEGGAPGTDVIDDGSTHPRRFHGTVVLDESRVGRDAGRIAEEVIAHLTGLVGTKV
                     TVTLEVEAEIPAGVPDHVVRTVTENSRTLKFTSQGFEKE"
BASE COUNT          459 a         1102 c         1022 g          548 t
ORIGIN      
        1 ccgctgcacg ttctgcgcca tgtacaagga caagcggttc cgcgtgcgcc ctctcgagga
       61 cgtgctcgaa gacctcgacc tcgcccgcgc caccttcgcc cacgtgccgg ccatcttctt
      121 ggccgacggc aacacgatag cactgcccac cgaccacctg ctcgcgatct tggcccacgc
      181 ccacaccctc ttcccagaac tggagcacgt gggcctctac ggcggagccc ggtaccttgc
      241 ccgcgacccc ggccccgacg acgcggggcg ccctggattg gcggccggca agagcgtcac
      301 cgaactcgcc gagctgcgcg gggcgggtct gagtgcggtc tacctgggcc ttgagagcgg
      361 cgacgccgag gtcctgcgca acgtcaagaa gggcgtgact ccggacgaca tggtcaaggc
      421 gggccgcagg ctgaaggaag ccgggctccc cgtcagcctg tacatactgg tgggtctcgg
      481 cggcctccac agataccgcg agcatgccct ggggacggcg cacgtcatca acgccatcgg
      541 gcctgacatc gtgcggccac gcaccctcta catccaagaa ggcacaccgc tgtggcacca
      601 gcggcggcgc ggagagttcg tggagaccgg cccgcgcgaa gcgcttctcg agacgcggct
      661 gctcctggag aacatcacgg tgccgttgac gctcctctcc gaccacatca ccaactacct
      721 tccactgcat ggacggctcc cagaagaccg cgaccggctg atcgacgcta tcgaccttgc
      781 gctggcgaac gacgacctcg actcgctgcg acctacccac ttcgatcacc tctgatcgat
      841 ggcggggcgg cgcgtgaggc gaggcgcgcg ttggggcccg cggcgtgctg cgcacctcgt
      901 cgccaccccg accgcacgcc ccggatgtat catcgctccg tgaggacggt cgatggtgtc
      961 cggaccgccc tcccaacgca agtccgagac gagacaatcg gcccgctccg cctgcgggcg
     1021 gtcagactgg aggcagcgat gcgcagcggc ttcgagacgg tccgcgacgc gtccgccccg
     1081 gccctctctc ccgtgcacca tccgcggcat ggccggcggc tccgttgatc gagtggcacc
     1141 tcatgctggc gatcgcatcc ataggcctgg tcgccgggtt cctgtccggg ctgttgggca
     1201 tcggcggcgg gataatcgtg accccgctcc tgctctacat gccgcagctc gtcggcgcat
     1261 cgacgctgtc catccgcact gtggcgggcc tgacgatcgt ccagggactt ctcgcctcac
     1321 tggtgggggc gatctctcac cgcaggttca agttctcgtc cggccgtctc gctgcataca
     1381 tgggcgccac catattcgtt gcggcgttcc tggggggagc gggatcgaag ctggtgtcca
     1441 acaccgtcct cctcgtactg ttcttggcgt tggcctgcgc gggcgggatc ctcatcctgc
     1501 tgcccacgaa gaacgaggag gagtaccccg ccggggccgc cctggatttc aacccggcgc
     1561 gggcggtggc ggtcgccgca ggcgtgggat tgctcggcgg catggtgggc cagggagggt
     1621 cgttcatact catcccgctc atgacctggt tcgtcaaagt gcccacccgc atagccatcg
     1681 gctcgaacct ggccgtggtg ctcttctcct cggcagccgg cttcctcggc aaggccttca
     1741 cgggccagat cgagtggttg ctggccgtcc cgctcgtgct gacggtgatc ccctcggccg
     1801 cattgggcag cagggtcagt cgcaggctct cggtgttgac gctgcggcgc actctcgccg
     1861 tggtcatcgg cgtcgcggtg gtgcgcatcg cggtctcact cctgagcggt gaggtcgccg
     1921 gctgatcgtc ggcggtccat gaccgcatcc ggcggcttgc gaaggtagcg aagccgaacg
     1981 tccaagggct cgcgcggccg ctagagccga gcctccgacc gcgcggtccc tacaacccca
     2041 gatcgcccgc gatcgccttc atcgcgtcca accccagcgc caggaactcc tccagaggga
     2101 tgccgagctc ctcgcaggtg gcgatgtcct cccgccgggc gcctcgcgcg aacgacggtt
     2161 ccttgaagcg tttcttcaga ctcttgagct ggacgagttc cagcttcttc tccggtcgga
     2221 tgagcgcggc ggccgtgacc agacccgtga gcggatcggc ggcgaagagc gcacgggcca
     2281 tagtcgtggt gatcgtggac ccgttcgccg ggttgtgcgc cagtatggcg tcgaggatct
     2341 cctcatcgtc gagaccctcc gcgcgaagcc actcgacggt gcgcgtggcg tggacggtca
     2401 tcatctcggc cgtctgctcg gcatccaggt cgtgaagcag gccggtgagc ccccagcggg
     2461 caggatcgcc gccgaggcgg ttggccagtg cgaccatgat ggcctccgcc gcgagcatgt
     2521 ggttcaccag gttttcgttg ccgacgcgct cgcggacgag cttgagtgct gcctgtcgtt
     2581 ccaacgggtg tgacctcctc gactacggga atcagccggc gaaagccggc gggccgagcg
     2641 gacggctatg tcagacgagc ggcccgagtg ggcgggtccg actcgactat ggcgcccccg
     2701 ccccctactc cttctcgaac ccctggctgg tgaacttgag cgtccgactg ttctctgtga
     2761 cggtccgcac cacgtgatcg gggactccgg cggggatctc cgcttctacc tcaagggtga
     2821 ccgtgacctt tgtcccaacc aggccggtga gatgcgcgat cacctcctcg gcgattcggc
     2881 ccgcgtcgcg gcccactcga ctctcgtcga gcacgaccgt gccgtgaaag cgtctggggt
     2941 gggtggagcc gtcgtcgatg acgtcagttc cgggcgcgcc gccctccgca cccggtgcgg
     3001 agccgcgtga cccatcgtct tccgcactag gaggtgggtc tggcttcgcg ctttcgacat
     3061 cgatttgtct acgtgcgacc tccggcttca ccacgagacc cgggcttgcc gcgtcgacca
     3121 ccgacacgat c
//
LOCUS       DASRMJ010000306         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_538130, whole genome shotgun sequence.
ACCESSION   DASRMJ010000306 DASRMJ010000000
VERSION     DASRMJ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538130"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..876)
                     /gene="sucD"
                     /locus_tag="VFD74_08450"
     CDS             complement(1..876)
                     /gene="sucD"
                     /locus_tag="VFD74_08450"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZK49615.1"
                     /translation="MAILIDQDSKIVVQGLTGREGSFHALRNRAYGTRVVAGVTPGKG
                     GQDVEGIPVFNTVREAVEATGADVSLIFVPPRFAADAMYEAIDSGVGLIVTITEGIPA
                     HDMLKVYNYLRVKGVSMIGPNCPGVISPGKANVGIMPPQIFAEGVVGLVSRSGTLTYQ
                     IANELTQKGLGQSTAVGIGGDPIVGSNFIDILTRFEADPQTKIIAMVGEIGGNEEERA
                     ADFITRHVTKPVVAYIAGFTAPPGKQMGHAGAIISGSAGTAGAKKRALEAVGIRVGVN
                     PTEVGSLVAEVAAAL"
     gene            complement(878..2029)
                     /gene="sucC"
                     /locus_tag="VFD74_08455"
     CDS             complement(878..2029)
                     /gene="sucC"
                     /locus_tag="VFD74_08455"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZK49616.1"
                     /translation="MDLHEYQGKELFASVGLPVPRGSVAGTPEEAERVAAELGGRVVV
                     KAQVHIGGRGKAGGVKLADTPAQAREVAEQIIGMDIRGHQVRQVLVEEASEIARELYA
                     ALVLDRGEKKVLAMLSGIGGMEIEQVAHDHPEALARVWIDPLAGWSDYHGRRLCFGAG
                     LGADMVRPIGDVFARLWDAFVRFEATLVEVNPLILTADGRLVALDAKFSVDNNSLYRH
                     QDIAAMRDVAAADPQEQMAAERGVTYVKLDGDIGVLGNGAGLVMSTLDVVADAGGAPA
                     NFLDVGGGAKADEIVSALEVITSDPKVKVIFFNIFGGITRCDEVARGVLTAIERMGID
                     MPIVVRLDGTNDVEGREIIETAHVSTLHVEATMLEAAHRAVALAAGMER"
     gene            complement(2111..>3301)
                     /locus_tag="VFD74_08460"
     CDS             complement(2111..>3301)
                     /locus_tag="VFD74_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZK49617.1"
                     /translation="TINSPAAILLLMYELVAEKQGADTKQLRGTIQNDVLKEYVARGT
                     YIFPPAPSMRIITDIFAYCQENLPTWNTISISGYHIREAGSSAAEELAFTLANGIAYV
                     QAAIDAGLEVDALAGQLSFFFNAHNNLLEEVAKYRAARRMWARVMRDRFGAVDPKSQM
                     LRFHTQTAGSTLTAQQPHNNIVRVALQALAAVLGGTQSLHTNSFDEALALPSDESVAI
                     ALRTQQIIGYESGLADIPDPLAGSFALETLTDDLEAKAQELMDEVDRLGGSVKAIEQG
                     YMQRAIEDSAYEYQRAIERGDRAVVGLNKFSTGEKPRFNLHRVDPTVQQLQEKRLTGL
                     RAERDEAAVTDALEAVRRTARSSENLLYPMKEALRLYATLGEITGVLRGEFGEFKGAG
                     ALHL"
BASE COUNT          516 a         1127 c         1058 g          600 t
ORIGIN      
        1 ctataacgcg gccgcgacct ccgctaccag cgaccccacc tcggtggggt tgacccccac
       61 acggataccc acggcctcga gggccctctt ctttgcgccc gcggtgcccg ccgatcccga
      121 gatgatggca ccggcgtgcc ccatctgctt gcccggaggc gcggtgaaac cggcgatgta
      181 ggccacgacc ggcttggtca cgtgtcgtgt tatgaagtcg gccgccctct cttcttcgtt
      241 gccgcctatc tcgccgacca tagcgatgat cttcgtctgg ggatcggctt cgaaacgggt
      301 gagaatgtcg atgaagttcg atcccacgat ggggtcgccg ccgatgccca cggccgtcga
      361 ttggccgagt cccttctgag tgagttcgtt ggctatctga taggtgagtg ttccggaccg
      421 gctcaccaga cccacgacgc cctcggcgaa gatctgcgga ggcatgatgc ccacgttggc
      481 cttgcccggc gagatgacgc ccgggcagtt gggcccgatc atcgagacgc ccttcacccg
      541 caggtagttg tacaccttga gcatgtcgtg agcggggatg ccctcggtga tggtcacgat
      601 gagcccgacg cccgagtcga tggcctcgta catggcgtcg gcggcgaagc gcggcggcac
      661 gaagatgagg gagacgtcgg cccccgtagc ctcgaccgcc tcgcgcacag tgttgaacac
      721 ggggatgcct tctacatcct gcccgccctt gccgggagtg acgccggcca ccactcgcgt
      781 gccgtaggca cggttgcgga gagcgtggaa cgacccttcg cggccggtga ggccctgcac
      841 cacgatcttg ctgtcttgat cgatcaggat ggccatctca gcgctccatt ccggccgcca
      901 gcgccaccgc ccgatgggcc gcctcgagca tggtggcttc cacgtgcagc gtggagacat
      961 gggcggtctc tatgatctcc cggccttcga catcgttggt tccgtcgagg cgcaccacga
     1021 tgggcatgtc gatgcccatc cgttctatgg cggtgagcac gccccgagcg acctcgtcac
     1081 aacgggtgat cccgccaaag atattgaaga agatcacctt gaccttgggg tcggaggtga
     1141 tgacctcaag ggcgctcacg atctcgtcgg ccttggctcc ccctcccacg tcgaggaagt
     1201 tcgccggtgc cccgcccgcg tcggccacca cgtcgagcgt gctcatcacc aaccccgcac
     1261 cgttgcccag cacaccgatg tcgccgtcca acttcacgta ggtcacacct cgctcggctg
     1321 ccatctgctc ctgcgggtcg gcggccgcga cgtcgcgcat ggcggcgatg tcctggtgac
     1381 ggtagaggct gttgttgtcc accgagaact tggcatcgag ggccaccagc cggccgtcgg
     1441 ccgtgaggat caacgggtta acttcgacga gcgtggcttc gaaccgcacg aaggcgtccc
     1501 acaggcgcgc gaacacgtcc ccgatggggc ggaccatgtc ggcgcccagc cctgcaccga
     1561 aacagaggcg ccgtccatgg tagtcgctcc accccgccaa gggatcgatc cacacacggg
     1621 cgagagcttc ggggtggtcg tgggccactt gctcgatctc catgccgcct atccccgaca
     1681 gcatggccag gaccttcttc tcgccccggt cgagcaccag cgccgcgtac agttcgcggg
     1741 cgatctcgga cgcttcctcc accagcacct gacgcacctg gtgcccccgg atgtccatgc
     1801 cgatgatctg ctcggctacc tcgcgcgcct gcgcgggcgt gtcggcgagt ttaacgccgc
     1861 cggccttgcc gcggccgccg atgtgcacct gggctttcac cacgacccga ccgcccagct
     1921 cggcggccac tcgctccgcc tcctccggcg tgccggccac cgaccccctc ggaacgggca
     1981 accccaccga ggcgaacagc tccttgcctt gatactcgtg cagatccatg cgtagtactc
     2041 cactccgtcg tgtgatgacg cccgggggcg cgccggtcgg ccgcctccgg acacccctct
     2101 catcccagcc ttacaggtgc aacgcccccg cacccttgaa ctcgccgaac tcccctcgca
     2161 agaccccggt gatctcgccc agggtggcgt agagccgcag ggcctccttc atcgggtaca
     2221 gaagattctc cgacgaacgc gcggtgcgcc gcaccgcttc gagcgcgtcg gtcacggccg
     2281 cctcgtcgcg ctcggcgcgc agtccggtca atcgcttctc ctggagttgc tgcaccgtgg
     2341 ggtccacccg gtgcagattg aagcgcggct tctctcctgt ggagaacttg ttcaagccca
     2401 cgacggcgcg atctccgcgt tcgatcgccc tctgatactc gtacgccgaa tcctcgatgg
     2461 cccgctgcat gtagccctgt tcgatggctt tgacggagcc acccagacgg tcgacctcgt
     2521 ccatcagctc ctgggccttg gcctcgaggt cgtcggtgag agtttcgagc gcgaaggagc
     2581 cggccagcgg gtcggggatg tcggccagcc ccgattcgta tccgatgatc tgctgggtgc
     2641 gcagcgcgat ggcaaccgac tcgtcggagg gcagcgcgag cgcctcgtcg aaggagttgg
     2701 tgtgcagaga ctgggtcccg ccgagcacgg ccgcgagcgc ctgcagcgcc acgcgcacga
     2761 tgttgttgtg cggttgttgg gcggtgaggg tcgaaccggc cgtctgcgtg tggaagcgga
     2821 gcatctggct cttcgggtcg accgccccga agcggtcgcg catgacgcga gcccacatgc
     2881 ggcgggcggc gcggtacttg gcgacttctt cgagcaggtt gttgtgcgca ttgaagaaga
     2941 aggagagttg tcccgccaaa gcgtcgacct cgaggcccgc gtcgatagcc gcctgcacgt
     3001 aggcgatgcc gttggccagg gtgaacgcca gttcctccgc cgccgacgac cccgcctcgc
     3061 ggatgtggta gccggagatg gagatagtgt tccacgtggg caggttctct tggcagtagg
     3121 cgaagatgtc ggtgatgatg cgcatcgagg gcgccggcgg aaagatgtag gtgccgcggg
     3181 ccacgtactc cttgagcacg tcgttctgga tggtgccccg gagttgcttc gtgtcagcgc
     3241 cctgcttctc ggccaccagc tcgtacatca ggagcaaaat ggcggccggg gagtttatgg
     3301 t
//
LOCUS       DASRMJ010000307         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_208664, whole genome shotgun sequence.
ACCESSION   DASRMJ010000307 DASRMJ010000000
VERSION     DASRMJ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208664"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VFD74_08465"
     CDS             <1..134
                     /locus_tag="VFD74_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HZK49618.1"
                     /translation="SKVVVAINTDPTAPIFDYADYGIVGDCLQVLPALTERVRAISS"
     gene            157..549
                     /locus_tag="VFD74_08470"
     CDS             157..549
                     /locus_tag="VFD74_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HZK49619.1"
                     /translation="MHTNVKVLLAKPGLDGHDRGAKVIAIALKEAGMEVIYMGLHQTL
                     EAIVAAAVQEDVDIIGLSILSGGHIPICRRLMELLAEEGIDDIAVCVGGVIPKRDVPV
                     LKEMGVKGIFPGGANLREMTETLKALAG"
     gene            560..2188
                     /locus_tag="VFD74_08475"
     CDS             560..2188
                     /locus_tag="VFD74_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZK49620.1"
                     /translation="MDVARYIKNEKDQIEDVILSSGIHVKPKYGPEDLEAVGFDYQKD
                     LGEPGEFPFTRNLFAEGYRTREWTTRQYTGFGTPRETNERFRLMIDHGQTGLNVAFDL
                     PTQMGLDSDHPLAWGEVGRVGMAVDSLRDFEVAFAGIDLHKVGAGLTINAVASIMLAM
                     YQAVAEKLGFDRTKISATPQNDILKEVLGRGAWIYALEPAVKLIGDTIEYSMTTMPRT
                     NPVSVCGYHIRESGATPAQEMAYAFLIANAYIDEVAKRGYDPEEFVGRFSFNFNIFGN
                     LWEQVAKFRAGRKVWAHNLSDRYQVKNPKNLWLRGLFAGGGSGLTKAEPENNIMRGAF
                     YGLAAALSGAQSTALCSFDEAFTIPTPRSALLSLRTLQILMDEVGMRDTVDPLAGSYF
                     IETLTKQMEEKIWEEMREIEEQGGVIKAITNGYLPRKLAWQAYEFERDVQKGDLIKVG
                     VNKYAEEEDADVDLHEYNEAWAEEKLADLVELKRTRDSAAVASSLKNLEAVARAGGNV
                     MPALVESCKAYATVGEMSDVFRDVYGVFKEPSIF"
     gene            2202..>4022
                     /locus_tag="VFD74_08480"
     CDS             2202..>4022
                     /locus_tag="VFD74_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZK49621.1"
                     /translation="MYKIGIDVGGTFTDFLLSRDDGTSSIYKVLSTPKDPSIATIQGI
                     EEMARDLGLSTADFLRRVVTIVHGTTVTTNATLTYRGAKTGLLTTRGVRDALEMRRGI
                     REEQYNNRYENARPVVERYLRLPVDERLDYAGNVVKQLDEGSVAAALAEFQRENVEAV
                     AICFMNSFANPAHERRAAELVREAMPDVFLSVSADVAPAIRFYDRVSTTALNAYIGPV
                     LTRYLTSLVARLESFSYEGILLIMASNGGVISPQSAMDRPAMTLLSGPAGGPVAGLAF
                     SQGQGYRDCITCDMGGTSFDVALIKDGEPLTTVEGEINRLRISLPMLNVVTIGAGGGS
                     IGWIDEVGLLRMGPQSAGADPGPACYGRGGQEPTCSDADLVLGYLDPDFFAGGRLKLD
                     AAAAAAAIEERVAKALGLSMEEAAAGMYEVINVSMAAAVREIAVNSGYDPRDFPLVTA
                     GGAGPNHACMIALALSIPVILVPRESSIFCAAGMLRSDLRHDFVRTYPVRVDLIDMAR
                     YQRLFTEMRDEGRGLLLSEGIGDDRISYQPNLELRYLRQYHEVNVPATWDEIMSGDFS
                     GIVSRFHTAHDTLYGYSLEDKGTPVELINMRLVAVGDTEKP"
BASE COUNT          729 a         1323 c         1308 g          662 t
ORIGIN      
        1 cctccaaggt ggtggtggcc atcaacaccg accccacggc ccccatcttc gactacgccg
       61 actacgggat cgtgggcgac tgtctgcagg tgcttccggc gctgaccgaa cgcgtccgtg
      121 ccatctcaag ctgacaggta agggagaagg gcagtcgtgc ataccaacgt gaaggtccta
      181 ctggcgaagc cgggactcga cggtcatgac cggggagcca aagtcatcgc catcgccctc
      241 aaggaagcgg gcatggaggt catctacatg ggcctccacc agacgctcga ggccatcgtg
      301 gccgcggccg tccaggagga tgtggacatc atcggcctct ccatcctctc cggagggcat
      361 atccctatct gccggcggct catggagctc ctggccgaag agggcatcga cgacatcgcg
      421 gtgtgcgtcg gtggggtcat ccccaagcgg gatgtcccgg tgctgaaaga gatgggcgtc
      481 aaggggatct tccccggcgg cgccaatttg cgcgagatga ccgagaccct gaaggcactg
      541 gcgggttagg agcctataga tggatgtggc gcgttacatc aagaacgaga aggaccagat
      601 agaggacgtc atcctctcct cggggatcca cgtgaaaccg aagtacggtc ccgaggacct
      661 ggaagccgtc ggcttcgact accagaagga cttgggcgaa cccggggagt tccccttcac
      721 ccgcaacctc tttgccgagg gctaccgcac ccgcgagtgg accacccgcc aatacaccgg
      781 cttcggcacc ccgcgcgaga ccaacgagcg cttccgcctc atgatcgacc acggccagac
      841 cggcctcaac gtggccttcg acctgcccac ccagatgggc ctcgactccg accatccgct
      901 cgcctggggc gaggtgggcc gggtgggcat ggccgtcgac tccctgcggg acttcgaggt
      961 ggctttcgcc ggtatcgacc tgcacaaggt gggtgccggt ctcaccatca acgcggtggc
     1021 ctccatcatg ctggccatgt atcaggccgt ggccgagaag ctgggcttcg atcgtaccaa
     1081 gatctccgcg actccccaaa acgacatcct caaagaagtc cttggacggg gcgcctggat
     1141 ctacgcgctg gaaccggcgg tgaagctcat cggcgacacc atcgagtact ccatgaccac
     1201 catgccccgc accaacccgg tctccgtgtg cggctaccac atccgcgaga gtggggcgac
     1261 tccggcgcag gagatggcat acgccttctt gatcgcgaac gcctacatcg acgaggtggc
     1321 gaagcgtggc tacgatcccg aggagttcgt aggacgcttc agcttcaact tcaacatctt
     1381 cggcaacctg tgggagcagg tggccaagtt ccgggccggc cgcaaggtgt gggctcacaa
     1441 cctgagcgac aggtatcagg tgaagaaccc caagaacttg tggctgcgcg gtctcttcgc
     1501 cgggggcggc tctggtctta ccaaagcgga acccgagaac aacatcatgc gcggcgcctt
     1561 ctacggcctg gccgccgccc tctccggggc gcaatccacg gcgctctgct ccttcgacga
     1621 ggccttcacc atccccaccc cgcgctcggc gctcctctcg ctgcgtaccc tgcagatcct
     1681 catggacgaa gtgggcatgc gcgacaccgt cgacccgctc gccggctcct acttcatcga
     1741 gacgctcacc aagcagatgg aagagaagat ctgggaggag atgcgggaga tcgaggagca
     1801 gggtggcgtc atcaaagcca tcaccaacgg ctacctcccg cgcaagctgg cctggcaggc
     1861 ctacgagttc gagcgcgacg tgcagaaggg tgacctcatc aaggtgggcg tcaacaagta
     1921 tgccgaggaa gaggacgccg acgtggacct ccacgagtac aacgaggcct gggctgagga
     1981 gaagctggcc gacctggtgg agctcaagcg cacccgggat tccgcggccg tggcgtcttc
     2041 gctgaagaac ttggaggccg tggcccgggc gggcgggaac gtcatgcccg cactggtgga
     2101 gtcctgcaag gcctacgcga ccgtggggga gatgtccgat gtcttccgcg acgtgtacgg
     2161 cgtgttcaaa gaaccgagca tcttctaagg gggctgggcc ggtgtacaag atagggatag
     2221 atgtcggggg caccttcacc gacttcctgc tcagtcgtga cgacggtacg agctccatct
     2281 acaaggtgct ctcgactccc aaagacccgt ccatcgccac catccaaggc atcgaggaga
     2341 tggcgaggga cctggggctg tccacggcgg acttcctcag gcgggtggtc accatcgtgc
     2401 acggcacgac ggtgaccacc aacgccactc tgacctaccg gggggccaag acggggctgc
     2461 tcaccacgcg cggcgttcgc gacgcgctcg agatgcggcg cggcatccgc gaggagcagt
     2521 acaacaaccg ctacgagaac gcgcggcccg tggtagagcg ctacctgcgt ctgcccgtcg
     2581 acgagcgtct ggactacgcg ggcaacgtgg tgaagcagct ggacgagggc tccgtcgcgg
     2641 ccgcgctggc cgaattccag cgggagaacg tggaggccgt cgccatctgc ttcatgaact
     2701 ccttcgccaa cccggcgcac gagcgccgcg ccgcggagct ggtgcgcgag gcgatgccgg
     2761 acgtcttcct ttcggtgtcg gccgatgtgg cgccggccat ccgcttctac gatcgggtct
     2821 cgaccacggc gctgaacgcc tacatcggcc ccgtgcttac gcgctatctt acgagcctgg
     2881 tagcgcggct ggagtcgttc agctacgagg gcatcctgct gatcatggct tcgaacggcg
     2941 gggtcatctc gccgcagtcg gcgatggacc gccccgccat gacgctcctt tccggtcccg
     3001 cgggcgggcc cgtggccggc ctggcgttct cacaggggca gggttaccgg gactgcatca
     3061 cctgtgacat gggcggcacc agcttcgacg tggccctgat caaggacggc gagccgctca
     3121 ccacggtgga gggcgagatc aaccggctgc gcatcagcct acccatgctg aacgtggtga
     3181 ccataggggc cggcggtggc tccatcggct ggatcgatga ggtcggcctg ctgcgcatgg
     3241 gcccgcagag cgcgggcgcc gatccgggcc cggcctgcta cggccgcggc ggccaggagc
     3301 ccacctgctc cgacgccgac ctggtgctgg gctacctgga ccccgacttc ttcgcgggcg
     3361 gacgtctcaa gctggacgcc gccgcggccg ccgccgccat cgaggagcgc gtggccaagg
     3421 cgctcggcct ctccatggag gaggccgcgg ccggcatgta tgaggtgatc aacgtgagca
     3481 tggcggcggc cgtgcgcgag atcgccgtga acagcggcta cgacccgcgc gacttccccc
     3541 tggtgacggc cggcggcgcc ggccccaacc acgcctgcat gatcgcgctt gcgctgtcca
     3601 tccccgtgat cctggtgccg agggagagtt cgatcttctg cgcggccggc atgttgcgct
     3661 ccgacctgcg tcacgacttc gtgcgcacgt atccagtgcg cgtcgacttg atagacatgg
     3721 ctcgctacca acgtctgttc acagagatgc gcgacgaagg ccgcggcctg ttgctgtcgg
     3781 aaggcatcgg cgacgatcgg atcagttatc agcccaacct cgagctgcgt tacctgcggc
     3841 agtaccacga ggtcaacgtg cccgccacct gggacgagat catgtccggc gatttctcgg
     3901 gcatcgtctc ccgcttccac accgcgcatg acaccctcta cggctactcg ctggaggaca
     3961 aaggcacgcc tgtcgagctg atcaacatgc gtctcgtggc tgtgggcgat acggagaaac
     4021 cc
//
LOCUS       DASRMJ010000308         3670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_315904, whole genome shotgun sequence.
ACCESSION   DASRMJ010000308 DASRMJ010000000
VERSION     DASRMJ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315904"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFD74_08485"
     CDS             complement(<1..252)
                     /locus_tag="VFD74_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49622.1"
                     /translation="MNAENSHTLDELAARIRHALACVADVLDLRLLTEQERAHIVGLE
                     TSTEQGGAAGGMVEFFNEGIRGALACNVVYAATTGPMLDD"
     gene            complement(392..2047)
                     /locus_tag="VFD74_08490"
     CDS             complement(392..2047)
                     /locus_tag="VFD74_08490"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZK49623.1"
                     /translation="MKKPPLTVRDLISEQEFQLRLLAGSAAIDRAVEGIHLSELEDPT
                     PWMAPNSILLTTGLNIKADPTAGVRLVELLSATGMAGLGVALEHYLTELPAAAVQRAE
                     ELAFPVFTLPKTVPFRRLLAYSYDALAARDTYRLRRSMSVQNHLLELLIEERGIPELV
                     QQLGHLLEATVMLFDGAGSLLTVAEGQARVSARGQATLWSRYLEHRDRKEPHPAFQSS
                     GFRVSYREASLHGAVERVLLAVYTNRPIPELAETTLSFAQKLLMLDVLRGRDVAALQR
                     RMRTGLMDDLVSGIGTETELASRMRQYSLDASRSWRLAVLDIDEFAERFGKTRRGAHR
                     EERIQEFKSRFLEATDAFLANQGLPFLSILKSDTVVTLIQFADPTPPGAERTLAALRA
                     RLEETLSPARISIGVSAAGVGAAPVALAFRQAREALGLTRQTGGLRIRFFDQIHPQNR
                     LLDAQSPEGLAAIYDETVRPLADHDAARRSKLLHTLEVFLAHERSLSRTATELHMHRN
                     TLAKRIARIEGILGRSLAATDDLVAVGLGLRARDLLEGRSSSR"
     gene            2328..>3670
                     /locus_tag="VFD74_08495"
     CDS             2328..>3670
                     /locus_tag="VFD74_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK49624.1"
                     /translation="MTTDSQMSAREMVELTKKHSFFSWSVQSQVSPIPMARAKGVYFW
                     DSDGKRYTDMNSQLMCVNIGHGDQRVIDAIKAQAQELVYAGPGMATRVRAEIGPMMAE
                     VTPGDLDTFFFTLGGAEANENAIKMARQFTGRHKIIARYRSYHGATAGAMTLTGDPRR
                     WANEPSIPGVIRAFDPYKYRSHLYAEGDSDQVFSQKCLDQMEEMMMYEGPQTIAAVYI
                     ESVTGTNGLIVPPDGYLQGLRALCDKYGILLVADEVMAGLGRCGEWFAVDHWDVVPDI
                     ITMAKGLTSAYLPLGAVAISGKIAEYYRDNKYYGGLTYNAHPMSLAAAVANLKVMHED
                     DLVGNARRMGAVMAGLMDDLKAKHPSVGDVRSIGLFGVIELVRNRVTKEPMAPFNGTS
                     PEMAKLGAFFKENGLFVFVNWNNFFSNPPLCITEAELREAYEIFDAGLAITDEAVT"
BASE COUNT          633 a         1187 c         1216 g          634 t
ORIGIN      
        1 gtcgtcgagc atcgggccgg ttgtggcggc gtagaccacg ttacaggcga gcgctccccg
       61 gatgccttcg ttgaagaact cgaccatccc cccggcggcg ccaccctgct cggtggaggt
      121 ctcgaggccg acgatgtggg cccgctcctg ctccgtcagc agcctgagat cgagcacgtc
      181 ggcgacgcag gccagggcat ggcgtattcg cgcggccaac tcatccagtg tgtgactgtt
      241 ctctgcgttc acgctgtcct atcctcatcg tgggtcgtcg gtggcgcgac ggttcgcggc
      301 caccccaggt tgtcggtgac gccaagattc gccgccaccc caggtcggcg gtggcgtcac
      361 ggtcgactgt cggtggcgcg gcggttcgtg gtcatctgga ggacgaccgt ccctcgagga
      421 gatcgcgagc cctcaacccg aggcctacgg ccaccaggtc gtccgtggcc gccaggctgc
      481 gtcccaagat cccctcgatg cgcgctatcc gtttcgcgag cgtgttccga tgcatgtgca
      541 gctcggtcgc cgttcggctc aacgagcgtt cgtgagccag gaagacctcg agcgtgtgga
      601 gcagtttcga gcggcgggcg gcgtcgtggt ccgcgagagg tcgcaccgtc tcgtcgtaga
      661 tggcagccaa gccctccgga ctctgggcgt cgaggagccg gttctgcggg tggatctggt
      721 cgaagaagcg gatccggagg ccgccggtct gtcgggtgag tcccagcgct tctctggcct
      781 gccggaacgc caaggcgaca ggtgcggcac ccacccccgc cgccgagacg ccgatgctga
      841 tgcgggccgg cgagagcgtc tcctccagcc gtgccctgag agccgccaac gtccgctcgg
      901 cgcccggagg cgtcggatcg gcgaattgga tgagcgtcac cacggtgtcg ctcttcagga
      961 tgctaaggaa gggcagcccc tggttcgcca ggaaggcgtc ggtggcttcg aggaaccggc
     1021 tcttgaactc ctggatgcgc tcttcgcggt gggctccgcg tctcgtcttc ccgaagcgct
     1081 cggcgaactc gtcgatgtcg agcacggcta ggcgccagga ccgcgacgcg tcgaggctgt
     1141 attgacgcat ccgcgaggcc agctccgtct cggtgccgat gccggacacc aggtcgtcca
     1201 tgagtcccgt gcgcatgcgg cgctgcaggg cggcgacgtc acgcccgcgc aagacatcga
     1261 gcatgagcag cttctgcgcg aacgacagcg tggtctccgc gagctccggg atagggcggt
     1321 tggtgtagac ggcgaggaga accctctcca ccgcgccgtg gagcgacgcc tcccggtatg
     1381 agacgcgaaa gcccgacgac tggaaagccg ggtggggttc tttacggtcg cggtgttcga
     1441 ggtagcgcga ccagagcgtc gcctgccctc gagcggagac gcgcgcttgt ccttcagcga
     1501 cagtgaggag cgagcccgcg ccgtcgaaga gcatgaccgt ggcctcgagc aggtggccga
     1561 gctgctgcac cagctcgggg atgccacgct cttcgatcag gagttccagc agatggtttt
     1621 gcaccgacat gcttcggcgc agccggtagg tgtcccgcgc cgccagcgcg tcatagctgt
     1681 aggccaggag tcgccgaaag ggcaccgtct tcggcagagt gaacacgggg aaggcgagct
     1741 cctcggcgcg ctgcacggcc gccgccggca actcagtcag atagtgctcc agagccaccc
     1801 cgagaccggc catgcccgtc gccgacagca gctctaccag ccggacgccg gcggtcggat
     1861 ccgccttgat gttgagtccg gtggtgagaa ggatgctgtt cggcgccatc cacggggtgg
     1921 gatcctcgag ttccgatagg tgtatgcctt ccacggcgcg gtcgatcgcg gccgagcccg
     1981 ccaagagtcg cagctggaac tcctgctcgg agatcagatc gcgtactgtg agaggtggtt
     2041 tcttcatgtg caccctgctg cggagagggt ctagtagtgt gcatgctacc actggaccgc
     2101 cggttcggcc cacagatagg ctgggtcggt catcgatacc tcatcggccc ctccgccgtc
     2161 gacgatgcca cgctcgaggc gaccgtcccg aagtaagtac tcttcggctc gtggcctgta
     2221 tcatggattt cgaaacgaac gccggtgcta tcggcacaga cgccggcgcg cccacggatg
     2281 ccgatgccgt gggcacctgg accgagaaca gagggaggca gcgtcgcatg accacagatt
     2341 cacagatgtc cgcccgggag atggtggagt tgacgaagaa gcactccttc ttctcctggt
     2401 ccgtgcagtc gcaggtgagc cccatcccca tggcgcgggc caagggtgtc tacttctggg
     2461 actccgacgg caagcgctac accgacatga actcccagct gatgtgcgtg aacatcggtc
     2521 acggcgacca acgcgtcatc gacgcgatca aggctcaagc ccaggaactc gtgtatgcgg
     2581 gccccggcat ggccacgcgg gtccgcgccg agatcggccc gatgatggca gaggtcactc
     2641 caggtgacct cgacaccttc ttcttcactc tcggaggcgc cgaggccaac gagaacgcca
     2701 tcaagatggc acgtcagttc accgggcgcc acaagatcat cgcccgctac cgttcctatc
     2761 acggcgcgac cgccggcgcc atgacgctca ccggggaccc acgccgctgg gccaacgagc
     2821 cgagcatccc gggtgtgatc agggccttcg acccctacaa gtaccgcagc cacctctacg
     2881 ccgaaggcga cagcgatcag gtgttctctc agaagtgcct cgaccagatg gaagagatga
     2941 tgatgtatga ggggccgcag acgatcgcgg cggtctacat cgagtccgtc acgggcacga
     3001 acggcctcat cgtgcctccc gacggctacc tccaaggtct ccgagccctc tgcgacaagt
     3061 acggcatact cctggtggcg gacgaggtca tggccggtct cggtcgctgt ggcgaatggt
     3121 tcgcggtgga ccactgggac gtggtgcccg acatcatcac catggcgaag ggcctcactt
     3181 ccgcctatct tcctttgggg gcggtggcca tcagcgggaa gatcgccgag tactatcggg
     3241 ataacaagta ctacggcggc ctcacctaca atgcgcatcc gatgtcgctg gcggcggccg
     3301 tcgccaatct caaggtcatg cacgaggacg acttggtggg caacgcccgc cgcatgggtg
     3361 ctgtcatggc cggtctcatg gacgatctga aagccaaaca cccgtccgtg ggcgacgttc
     3421 gctcgatcgg cctgttcggc gtgatcgagt tggtgcggaa ccgcgtcacg aaggagccga
     3481 tggcgccgtt caacggcacc agcccggaga tggccaagct gggcgcgttc ttcaaggaga
     3541 acgggctctt cgtcttcgtc aactggaaca acttcttcag caacccgccg ttgtgcatca
     3601 cggaagccga gctacgcgag gcctacgaga tcttcgacgc cggtcttgcc atcacggacg
     3661 aagccgtcac
//
LOCUS       DASRMJ010000309         4659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_501070, whole genome shotgun sequence.
ACCESSION   DASRMJ010000309 DASRMJ010000000
VERSION     DASRMJ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501070"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1116
                     /locus_tag="VFD74_08500"
     CDS             142..1116
                     /locus_tag="VFD74_08500"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZK49625.1"
                     /translation="MSTRLGLLELAHPLINASGTFDLFEVAESLGMDGDGAGVRLLAD
                     PPFSAYVPKTVTLEPRRGNEPPRLLETEAGLLNSIGLPNPGVEVFVTRELPRLLALPR
                     PVIMNVGGFSLEEYVATVARIRRELDGSAGSLVGGRPWTERVGLELNISCPNVHSGCM
                     SIGTDPAETAALVEAVRPVWPGLLLAKLTPNVTDIASVARAAQHAGADALSLVNTFKG
                     LALDRASLRPYLGGGTGGLSGPAIKPLALRCVYEVFEAVTVPIVGMGGVTDVRDVVDF
                     LACGASVVAIGSAVFRDPWAARRLAQELEEYLGAHDLSLADLIGRAHR"
     gene            1161..1493
                     /gene="mihF"
                     /locus_tag="VFD74_08505"
     CDS             1161..1493
                     /gene="mihF"
                     /locus_tag="VFD74_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HZK49626.1"
                     /translation="MTTSPVISAQTPERSHQQRIDALQRANGIRTERARLKQSLKSGA
                     VHIHDVLVEPPEYVHTAKVFDLILAVPKYGRVKTSRILERCRVSPSKTVIGLTPRQRR
                     ELVEHLKG"
     gene            1514..2098
                     /gene="gmk"
                     /locus_tag="VFD74_08510"
     CDS             1514..2098
                     /gene="gmk"
                     /locus_tag="VFD74_08510"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZK49627.1"
                     /translation="MIVISGPSGAGKGTLIARVLPQFQHLVKSVSATTRLQRDRELDG
                     REYIFITRDEFLRRVEQGEFLEWAEYGGNLYATPAAAVQAALDRGTDVILEIELQGAR
                     QVMERVSDAATVFIAPPSLRELEERLRRRATETEETIGLRMEHARDELEELARDALRK
                     SREFHYVIVNGDVDIAAGELRSAIEDIRRNDSTR"
     gene            2107..2376
                     /gene="rpoZ"
                     /locus_tag="VFD74_08515"
     CDS             2107..2376
                     /gene="rpoZ"
                     /locus_tag="VFD74_08515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZK49628.1"
                     /translation="MYKPTVDEIIKGLTEHMDENDEALTRYTAVMVAARRARQINSYY
                     RSLGEGAGFEDLAPPLIHTSSRNYLTIAMEEVARGEITYKLRTEH"
     gene            2417..3646
                     /gene="coaBC"
                     /locus_tag="VFD74_08520"
     CDS             2417..3646
                     /gene="coaBC"
                     /locus_tag="VFD74_08520"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZK49629.1"
                     /translation="MTGRPTVLLGVTGGIAAYKSAELLRGFQKAGMEVRVIMTEAATR
                     FIAPLTFETLSGYPVHVGDLLRADSTIAHIEAGNAADIFVIAPATANTLAKLANGLAD
                     NLLSATYLAYSGTVVVAPSMNTNMWNHLSTQRNVATVREHGAFVLEPGAGELACGVYG
                     SGRMADPDSIVAEVKHRLQVVRKTDLRGLRVVITAGGTREPIDAVRFMGNRSSGKMGV
                     ALARAAFDRGASVDLVKANVDLPLAPGVREYDTPTARAMYDKVMQLLPDTDILIMAAA
                     VADYQAVDAAVGKMGKSQDELMVRLEPTKDILCEVASHRSHGQIIAGFAAEFGDFGVK
                     RAREKLLRKRLDLIVVNDISRADIGFNSDYNEVVILMPRGETKVSRAPKETIAEIILD
                     HIAREARARRGDPTPLP"
     gene            complement(3842..>4659)
                     /locus_tag="VFD74_08525"
     CDS             complement(3842..>4659)
                     /locus_tag="VFD74_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK49630.1"
                     /translation="DPLRLYLKEAGETPLLTKSDEIQLAKRIEAGDKPAKDRMILSNL
                     RLVISIAKNYHAQDMDLLDLIQEGNTGLIRAVEKFDYRKGYKFSTYATWWIRQAITRA
                     IANQDRNIRIPVHMIEKINKMVRTERKLLQETGREPTDEELGDELGVDAQEVREMRKI
                     AQRTTSLETPIGDEEDAELGDFIENSSTPDPAEAVHSIIAKESLDRVLRTMDERERKV
                     VELRFGLKGEHPRTLAEVSSRFNVSRERIRQIEAKALETIKNSVDIQAMRESA"
BASE COUNT          798 a         1432 c         1580 g          849 t
ORIGIN      
        1 cggtcggctc gtgcgggtct gtcgcgaggg ccccgtgttc tccggagaag aggcgttcgg
       61 agatgccccg cattcctgcg cggcggaggg ggaggcactg tgaaggctcg aagggggcgg
      121 gaagaacggc gcacgtccgc attgagtact cgcctggggc tgctcgagtt ggcgcatccc
      181 ttgatcaatg cttcgggcac attcgatctg ttcgaagtgg cggagagtct cgggatggat
      241 ggcgacgggg cgggcgtccg gctgttggcg gatccgccgt tctcggccta cgtgcccaag
      301 acggtgacgc tggaacctcg ccgggggaac gaacccccgc gcctactcga gacggaagcg
      361 ggtctgctta actccatcgg tctgccgaac cccggggtag aggtgttcgt gacgcgggaa
      421 ctccctcgac tcctggccct gccccggccg gtgatcatga acgtgggggg cttctctctc
      481 gaggagtacg tggcgacggt agcgcgcatc cggcgggagt tggacgggtc ggccggatcg
      541 ctcgtggggg gaaggccttg gacggagcgc gtgggcctgg agctcaacat ctcctgcccg
      601 aacgttcact ctggatgcat gtccataggg accgacccag ccgagaccgc cgctctcgtc
      661 gaggcggtgc ggcctgtgtg gccgggtctc ctgctggcga agctgactcc caacgtcacg
      721 gacatcgcct cggtcgcccg agcggcacaa cacgccggtg cggatgcgct ctcgttggtg
      781 aacaccttca agggcttggc gctcgatcgc gcgtccctgc ggccatacct gggtggcgga
      841 accggtggcc tctcggggcc cgcgatcaaa cccctcgccc tgcggtgcgt ctacgaggtg
      901 ttcgaggcgg tcacggtgcc gatcgtgggg atgggtggcg tcaccgacgt gcgcgatgtg
      961 gtggacttcc tggcctgcgg cgcctcggtg gtggcgatcg gctccgccgt cttccgcgat
     1021 ccgtgggcgg cgcgccgttt ggcccaggaa ctcgaggagt acctgggcgc ccacgatctg
     1081 agcctggccg acctcatcgg ccgcgctcat cgatagacct ggttgcgccg cgtcgacgca
     1141 tgcgcaataa tcacgcgtac atgaccactt ccccagtgat atcggcccag actccggagc
     1201 gtagccacca gcagcgtatc gacgctctgc agagggccaa cggcatccgc acggaacgtg
     1261 cgcggttgaa gcagtcgctc aagagcggtg cggtgcacat ccacgacgtg ctggtggagc
     1321 ccccggagta cgttcacacg gcgaaagtct tcgatctgat cctggcggtt ccgaagtacg
     1381 gtagggtcaa gacctctcgc atactcgaac ggtgccgtgt gagcccgtcc aagaccgtca
     1441 tcggcctcac gccgcgccag cgacgcgagt tggtagaaca cctcaaaggc tgacggggta
     1501 gacggtcgcc aagttgatcg tcatctccgg gccttccggt gcgggcaagg gcactctcat
     1561 cgcccgcgtc ctcccacagt tccagcacct ggtcaagtct gtgtcggcca ccactcgcct
     1621 ccaacgggat cgagagctgg atggtcgcga gtacatcttc atcacgcgcg atgagttcct
     1681 ccgcagggtc gagcagggag agttccttga atgggccgag tacggcggga acctttacgc
     1741 cacgccggcg gcggcggtgc aggccgccct cgaccgaggc acagacgtga tcttggagat
     1801 tgagttgcag ggtgcgcgcc aggtcatgga gagggtgtcg gacgccgcga ctgtgttcat
     1861 cgccccgccg tctctgcggg agttggagga gcgcctgcgt cgtcgcgcca cggagaccga
     1921 ggagaccatc gggctgcgca tggagcacgc ccgcgacgag ctcgaggaac tcgcgcggga
     1981 cgccctgagg aaaagcagag aattccacta tgttatagtg aacggcgacg tcgatatcgc
     2041 cgcgggcgaa ctgcgctccg cgatcgagga tatccgaagg aacgactcaa cgaggtgatt
     2101 gcagggatgt acaagcccac ggttgacgag atcatcaaag gtctcacgga gcacatggac
     2161 gagaacgacg aagccctcac tcgctacacg gcagtgatgg tcgcggctcg gcgggcgcgt
     2221 cagatcaaca gctactatcg cagtctgggc gaaggcgccg gcttcgagga cctcgcgccg
     2281 ccgctgatcc acacgagctc ccgcaactac ctcaccatag ccatggaaga ggtcgcccgc
     2341 ggggagatca cctacaagct ccgtacggag cactaggcac cgggcgggtg gcgacacccc
     2401 gtcttgtgag ggcacgatga cgggccggcc cacggttctt cttggggtaa ccggcggtat
     2461 agccgcctac aagtcggcgg agctgctgag gggctttcag aaggcgggca tggaagtgcg
     2521 cgtcatcatg acggaggcgg ccacccgctt catcgcgccg ctcaccttcg agacgctctc
     2581 cggctacccc gtgcatgtgg gtgacctgct gcgggcggac tcgaccatcg ctcatatcga
     2641 agcggggaat gcggccgaca tcttcgtgat cgcgcccgcg acggcgaaca cattggcgaa
     2701 actcgccaac ggactggcgg acaacctcct gagtgccacc tatctagcct actccggcac
     2761 ggtcgtggtg gcgccctcca tgaacaccaa catgtggaat cacctgtcga cgcaacggaa
     2821 cgtagccacc gtgcgggagc acggggcgtt cgtactcgag ccgggggcgg gtgaattggc
     2881 gtgcggggtc tacggttccg ggcgcatggc tgatccggac agcatcgtgg ccgaggtgaa
     2941 gcaccgcctg caggttgtgc ggaagacgga ccttcgcggc ctgcgcgtgg tgatcaccgc
     3001 cggaggcaca cgagagccca tcgacgcggt gcgtttcatg gggaaccgca gctcgggcaa
     3061 gatgggcgtg gcgctcgccc gcgcggcctt cgaccgtggg gcctcggtcg acctggtgaa
     3121 ggcgaacgta gacctgccgc tcgcgccggg tgtgagagag tacgacaccc ccacggcgcg
     3181 cgcgatgtac gacaaggtga tgcagctcct gccggacacc gatatcctca tcatggccgc
     3241 cgctgtcgcc gactatcagg ctgtcgatgc ggccgtgggt aagatgggga agagccaaga
     3301 cgagctcatg gtgcggctcg agcccacgaa ggacatcctc tgcgaggtcg cctcccaccg
     3361 cagccacggg cagatcatcg cgggattcgc ggccgagttc ggagatttcg gcgtgaagcg
     3421 ggcccgcgag aagctgctgc gcaagcgact cgatctcatc gtggtcaacg acatctctcg
     3481 tgcggacata ggcttcaact ccgactataa cgaagtcgtg atcctgatgc cgcgaggtga
     3541 gaccaaggtg agtcgtgcgc cgaaggagac catcgcggag atcatcctgg accacatcgc
     3601 ccgtgaggcc agagcccgcc gcggcgaccc cacgcctctg ccgtgagacg acaggaggtc
     3661 ctgttccgca tggccacgga cgtggacggc ttcagagggt tcttcgaaag ccagggattg
     3721 gtggcctagc ggtcgcggct accggcgaac gagaagaggc gccgctccca ggagcggcgc
     3781 ctctttgtgc acgtgcggcg cgtctctccg gtcaggggac cggcatagac tccggatctc
     3841 ttcaggcact ctcgcgcata gcctggatat ccaccgagtt cttgatcgtc tcgagagcct
     3901 tggcttcgat ctgcctgatg cgctcgcggc tcacgttgaa gcgggaactg acttcggcaa
     3961 gcgtgcgggg atgctctccc ttgagtccga agcggagttc cacgaccttg cgttcccgct
     4021 cgtccatggt gcggagcact cggtcgaggc tctccttggc gatgatgctg tgcacggctt
     4081 cagccggatc gggcgtgctg ctgttctcta tgaaatcgcc caactcggca tcttcctcgt
     4141 caccgatcgg ggtctcgaga gaggtggtgc gctgggcgat cttccgcatc tcgcgcactt
     4201 cctgagcgtc gacgccgagt tcgtcgccga gttcctcgtc ggtcggttca cgcccggtct
     4261 cttggaggag tttccgttcg gtgcgcacca tcttgttgat cttctcgatc atgtgcaccg
     4321 ggatgcggat gttgcggtcc tggttggcga tagcccgcgt gatggcttga cggatccacc
     4381 aggtggcgta cgttgagaac ttgtagcctt tgcggtagtc gaacttctcc accgcgcgga
     4441 tgaggccggt gttgccctcc tggatgaggt cgagcaggtc catatcctgg gcgtggtaat
     4501 tcttggcgat agagatcacc aggcgcaggt tggacaggat catgcggtcc ttggccggct
     4561 tatcgcctgc ttcgatgcgc ttggcgagct ggatctcgtc actcttggta aggagagggg
     4621 tctccccagc ttctttcaaa tataggcgaa gagggtcgt
//
LOCUS       DASRMJ010000310         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_539066, whole genome shotgun sequence.
ACCESSION   DASRMJ010000310 DASRMJ010000000
VERSION     DASRMJ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539066"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..352
                     /locus_tag="VFD74_08530"
     CDS             110..352
                     /locus_tag="VFD74_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49631.1"
                     /translation="MLCNLAVGVMEELQSDHAGAFSFAVVDASTRDGVKRLQELRPGA
                     GRQIPVPGVVVDGAIVFDRIPDMDEFGEWLDAATST"
     gene            401..1678
                     /locus_tag="VFD74_08535"
     CDS             401..1678
                     /locus_tag="VFD74_08535"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HZK49632.1"
                     /translation="MRELGHITLLIDRMADAPDFEAAAQALIEWARELTGCESAFVRM
                     RREGGQGRGWMPVVAASGASSDFLRDEAVVTDSECMCGRVSVGTIDADRPYFTDEGSF
                     LWGRVGSIFSEFEPATLGSMRSRCVLEGYESLAIIPLVGADGTVGCIHLADSRPDLFA
                     ETRELLEAAGRMAGRLLHNHREQDSERAALETIREALLPTRPPSVADVELGVFATTAE
                     VLMRVGGDFYDAFELPDGKVALVAGDYSGKGLDAVGAAANLRYHLAALVASAEKPGAF
                     LQQANVTLSDVLSPRRFATAICCALDPRNGRLEVALAGHPAPLLRRKGRVIELDAPAN
                     LPLGVAVDGLFETASFSLRKDDLLVLYTDGVSEARHEGSLFGIEGIARIIMDDPDSPL
                     AETARAVVTASGAHHDPALPDDDRLVLLARTLR"
     gene            1785..2816
                     /locus_tag="VFD74_08540"
     CDS             1785..2816
                     /locus_tag="VFD74_08540"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZK49633.1"
                     /translation="MLSKKYRVAVVGATGAVGQEMREILASRSFPVSELVPLASSRSA
                     GMELEFGTTCVECKELAEDSFVGIDIALFSAGASISEVYAPIARDAGCVVIDNSSAWR
                     MDPDVPLVVPEVNPDAAALHRGIIANPNCSTIQMVVALKPLHDEATITRVVVSTYQAV
                     SGTGTEAISELIRESDELLEGVLPQIEVYPYQIAFNCLPHIDSFLEDGSTKEEQKMVD
                     ETKKIMGDDSIAVTATCVRVPVENGHSEAVNVEFARGITPERARELLERMPGVLVTDD
                     PASSLYPMPIVASGTDPVYVGRIRRDPTVPHGLNMWVVADNLRKGAALNAVQIAELLI
                     ERDLVRVPH"
     gene            complement(2831..>3187)
                     /locus_tag="VFD74_08545"
     CDS             complement(2831..>3187)
                     /locus_tag="VFD74_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK49634.1"
                     /translation="LVVEDEPAVLRLTTRMLSSLGYQVFGADNGEAALRVMEIEGPRI
                     QCVVTDVLMPKMTGPRMVSLLRETHPELPVVFMSGYVLEADGDLMGAVEDAPWFIPKP
                     FTADQLARTVRSALDA"
BASE COUNT          523 a         1070 c         1068 g          526 t
ORIGIN      
        1 gccaggtccg cgggacactg ggccaggtcc gcgggacagt gggccaggtc cgcgggacag
       61 tggggcggga gcaccgcata tcgaccttat ctccaagggt ccgtcgtgag tgctctgcaa
      121 cctcgccgtg ggcgtgatgg aggaactgca aagtgaccat gccggagcct tctcgttcgc
      181 cgtcgtcgac gcttccacac gagacggcgt caagcgactc caggagttga ggccgggggc
      241 cggccgacag atccccgtgc ccggagtcgt ggtcgacggg gccatcgtgt tcgatcgcat
      301 ccctgacatg gacgagttcg gggagtggct cgacgccgcg acgagcacgt aggccaaccg
      361 ggtagacctt ccgggccgat ttcttctaca atcggcgtct atgcgcgaac tgggccacat
      421 cactctgctg atcgaccgga tggccgacgc cccggacttc gaggcggccg ctcaagcctt
      481 gatcgagtgg gcgcgggaac tcaccggttg tgagtcggcg ttcgtgcgca tgaggcgcga
      541 aggcggtcag ggccgcggat ggatgcccgt ggtggccgcg agcggagctt cttccgactt
      601 cctccgtgac gaggccgtcg tgaccgactc cgagtgcatg tgcgggcggg tctccgtggg
      661 caccatcgat gcggatcgcc cctacttcac cgatgaaggc tccttcttgt gggggcgggt
      721 cgggtccatc ttctcggagt tcgagccggc cacgctcggc tcgatgcgct cgcgctgcgt
      781 gctcgagggg tacgaatcgc tggccatcat ccctctggtg ggcgctgatg gcaccgtggg
      841 ctgcatccac ctcgccgaca gccggccgga cctgttcgcg gagactcggg aactgctcga
      901 ggcggccggc aggatggccg gccgcctcct tcacaaccac cgagaacaag actccgagcg
      961 ggccgcgctc gaaacgatca gggaagccct gcttccgacc cggcccccgt cggtggcgga
     1021 cgtggaactc ggagtcttcg cgaccaccgc tgaggtgttg atgcgtgttg gcggggattt
     1081 ctacgacgca ttcgaactcc ccgacggcaa ggtggcgctg gtggcggggg actactccgg
     1141 caagggcctg gacgcggtgg gcgccgcggc gaacctgcgc taccacttgg cggcgctcgt
     1201 ggcgagtgcc gagaagcccg gcgccttcct gcagcaggcc aacgtcaccc tctcggacgt
     1261 gctctcaccg cgccgtttcg ccactgccat ctgctgcgcc ctcgaccctc gcaacggtcg
     1321 gctggaggtg gccctcgccg gccatccagc cccgctgctg cggcggaagg ggcgagtgat
     1381 agagctggat gctccggcaa acctgcccct gggcgtcgcc gttgatggtc tgttcgagac
     1441 cgcatcattc tcgctccgga aggacgacct cctggtgctc tataccgacg gcgtgagcga
     1501 ggcgcgtcac gaaggcagcc tcttcgggat cgagggcatc gcgcggatca tcatggacga
     1561 ccccgactcc cccctggccg agaccgctcg tgcggtggta acggcctcgg gggcccatca
     1621 cgatccagcc ctacccgacg acgatcgctt ggtcctgctc gcgcgaacgc tccgctgacg
     1681 tctcagacgc gcgaaacacc tggtggtaga atcgacacgc tagtgacgct tgataccgct
     1741 ggatatcgat gtcgcctcgg gccgctggcc cgacgaggag gggactgttg agcaagaagt
     1801 acagggtcgc cgtggtgggt gccactgggg ctgtgggcca ggagatgcgg gagatcctcg
     1861 cgagtaggag tttccccgtc tcggagctgg tgccgctcgc ctccagccgg tccgccggga
     1921 tggagctgga gttcggcacg acgtgcgtcg agtgcaagga actcgccgag gatagtttcg
     1981 tcgggatcga catcgccctg ttctccgcgg gcgcctccat ctcggaagtc tacgccccca
     2041 tcgcccgtga cgcgggctgc gtcgtcatcg acaactcgtc ggcgtggcgc atggacccgg
     2101 acgtccccct ggtggtgccc gaggtcaacc cggacgcggc ggcgctgcat cgtggcatca
     2161 tcgccaaccc gaactgctcg accatccaga tggtcgtcgc cctcaagccc ctgcacgatg
     2221 aagcgacgat cacccgcgtg gtggtctcca cctaccaggc cgtctccggc accgggacgg
     2281 aggccatcag cgagctgata cgcgaatcgg acgaactgct cgagggtgtg ctgccgcaga
     2341 tcgaggtcta tccctaccag atcgccttca actgcctgcc gcacatcgac tccttcctcg
     2401 aggacgggtc caccaaggaa gagcagaaga tggtggacga gaccaagaag atcatgggcg
     2461 acgactccat cgcggtgacg gccacctgcg ttcgggtgcc cgtggagaac ggccactcgg
     2521 aggccgtgaa cgtcgagttc gcacgcggta tcaccccgga gagggcacgg gagctgttgg
     2581 agcgcatgcc cggagtactg gtgaccgacg accccgcgtc cagcctctac cccatgccca
     2641 tcgtcgcatc aggcacggac cccgtgtacg taggccggat ccggcgcgac cccacagtcc
     2701 cccacgggct gaacatgtgg gtggtcgcag acaacctgcg caaaggggcc gccctcaacg
     2761 cggtccagat agcggagctc ttgatcgagc gagacctggt gcgcgtgccc cactaggcgc
     2821 gccgtcgcgg tcaagcgtcg agcgccgacc tcaccgtccg ggccagctga tccgccgtga
     2881 acggtttcgg tatgaaccaa ggagcatcct cgaccgcccc catcaggtcg ccgtcggcct
     2941 ccagcacgta gcccgacatg aacaccacgg gcaactcggg gtgcgtctcg cgcaggagcg
     3001 acaccatgcg agggccggtc atcttgggca tgagcacgtc ggtgacgaca cactggatgc
     3061 gcgggccttc gatctccatc acccgcaggg cggcctcgcc gttgtcggcc ccgaacactt
     3121 ggtaccccag cgagctcagc atccgcgtcg tgagtcgcag cacggccggc tcgtcctcga
     3181 ccaccag
//
LOCUS       DASRMJ010000311         8568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_247851, whole genome shotgun sequence.
ACCESSION   DASRMJ010000311 DASRMJ010000000
VERSION     DASRMJ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247851"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="VFD74_08550"
     CDS             <1..1660
                     /locus_tag="VFD74_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZK49635.1"
                     /translation="ADAVARWTICSRSPLTGFWGDANVGGYFGAELKSAGYDGVVITG
                     AAAAPVYVAIHDGEVEIRDAADYWGADVYDTTDRLTGDLRREGGRAGQVLAIGPAGER
                     LVRYAAIANRKRHWAGRAGLGAVWGAKRLKAIYVRGTGSVPIAHPERFSALRAELKAL
                     YADDLLIEALRAFGTIASIDLGTMTGDVPYQNWRSGHWNGIDELSSVAYQERIYSGSA
                     GCHRCGVRCKREARVTEGPYAGVDGPGPEYETLAVFGPLCLNSNLEAIAKANELCNRL
                     GLDTMTCGSTIAFALECIENGLLDAAEWPGPTADWGDHEGIVALVEQIGRREGLGDLL
                     AEGSARVAARLGGRAFEFVSTVKGLEAPMHDPRSAHGLGLAYGVSARGACHNASLQYP
                     IEPGGMFVEDVPDLAVELPEQSSVGKAAMNVATQDYGVFFAGCAGFCNLGARPLSAAQ
                     AVEMIDSVTGAGYTLEELLTVGRRVWLLTRGLSNLFGARAADDNLPPRLLQALDEGPT
                     AGGSPDMDLMLSEFYKLRGLDERGIPRRDVLEAVGLRDLADLLE"
     gene            complement(1717..2703)
                     /locus_tag="VFD74_08555"
     CDS             complement(1717..2703)
                     /locus_tag="VFD74_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK49636.1"
                     /translation="MNRQLMVKVGARSLVNRVKRWPICVSFEVTHSCTADCLHCDKGR
                     LKREEGLLQPEDYARWSRQLRPAVTQLSGGEPLTRPDLEEIARAIRRWSGLPLIVCVS
                     NGWALTEERFRSLKNAGVDVFSISLDFPDERHDSFRQLRGLFARLDETVPKLTRLPGG
                     DAIVLNTALTSANFREIPGLVERAEQWGTKISFSAYSHLRTGDRGYSISDPEDLEVLA
                     DHFRYLRERRRATGSVLNSDYVLATTLDFFKSGGRGGCMAGLRFLVIRPDGLINPCSM
                     HPDLVYPTREAAVRGFDVRHTCKDCFVSIRSMTERPFIPLATDSVATFFQLQ"
     gene            complement(2700..3365)
                     /locus_tag="VFD74_08560"
     CDS             complement(2700..3365)
                     /locus_tag="VFD74_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49637.1"
                     /translation="MFAGRSTGSPGTRAVVFTIVTIALGLATVALRASGGASPIHPQL
                     LLLLYSIPANTGLSLFSHEVALLDYGAHQPLLITTLSATVGTVIAGYLDWRIFVPLFG
                     WDRLGGYVERRWVRGLLARFQRAPFFILVLTAASPIPFFPFKMMAFSVRYPMRRYLTA
                     LTVARAPRYLLIAWLGETLRLPAWLLLSILVAVLLIGVLKQVADHGLPAIRGRLRMRR
                     QQV"
     gene            complement(3457..4500)
                     /locus_tag="VFD74_08565"
     CDS             complement(3457..4500)
                     /locus_tag="VFD74_08565"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HZK49638.1"
                     /translation="MRIAMLTGGGDCPGLNAVIRAATRRAIASYGWEVFGIRNGWLGL
                     VDGDVQRLELGSISGILHEGGTILGTSRTNPFKREGRVEAVLETVRAHAIEAVVVIGG
                     EDTLGVALRLHDEYGLKVVGVPKTIDNDLSGTDYTFGFDTAVHVATEAVDRLHSTAES
                     HNRVMVVEVMGRQTGWIAVMAGIAGGADAILIPEFPMTVEEVCGLVQQRADRGKRFSI
                     VVVSEGISLLSGSGTAQTVVSSDEVDAFGHVRLGGVGTVLAREIERITGIEARVTILG
                     YIQRGGTPTARDRVLATRYGVKAAEMVKAGEWGMMAALRGDAVISVPLSEALADLKRV
                     PRELYDIARTFFG"
     gene            complement(4574..4732)
                     /locus_tag="VFD74_08570"
     CDS             complement(4574..4732)
                     /locus_tag="VFD74_08570"
                     /inference="COORDINATES: protein motif:HMM:NF045255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49639.1"
                     /translation="MEADLVDSTSAARVNGYLDGYGTIEQLEWEIDRLGLSASLSERL
                     RAEVSRRR"
     gene            complement(4790..5143)
                     /locus_tag="VFD74_08575"
                     /pseudo
     CDS             complement(4790..5143)
                     /locus_tag="VFD74_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            complement(5272..5688)
                     /gene="msrB"
                     /locus_tag="VFD74_08580"
     CDS             complement(5272..5688)
                     /gene="msrB"
                     /locus_tag="VFD74_08580"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZK49640.1"
                     /translation="MGRRAVTKTDDEWRRTLTPDQFRVARCKGTEPAFTGAYHDFKGD
                     GIYHCVACGSELFDSLAKYDSGSGWPSFYEPIEPERVEEQVDVGLFEPRTEVVCSRCG
                     SHVGHVFPDGPPPTGVRYCLNSLALEFRPRDRGEAR"
     gene            complement(5934..6956)
                     /locus_tag="VFD74_08585"
     CDS             complement(5934..6956)
                     /locus_tag="VFD74_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZK49641.1"
                     /translation="MKARFCAQCGEEVVVRGEDGRDREVCPRCGTVFYVNPLPVVASV
                     VLDHERRVLLVRRGQEPHEGMWCLPIGFAEIGETIQDAALRELREEAGIEGQVRILLD
                     VDSFESSHYGDLLIVSFEVEKTGGREVAGDDAVDVGYFSLDDLPPLAFPSNDRAIEAC
                     AARHEEEWRIQDSFHHLRERRGELLSDPLVEMIEQHASEIARRWAREMLAHPTTVCLR
                     GRAPDLLEKEATTALSRLGSWLRVEPEAQAPGVMSWGAAEVRAFYRTWGAERARTGCA
                     AHEVLSSLSLLKRSVYDFAHEVGVWERPADLYRVVELMSRIGAFFDRAQYHAARGFED
                     VRDSSK"
     gene            complement(6969..7799)
                     /locus_tag="VFD74_08590"
     CDS             complement(6969..7799)
                     /locus_tag="VFD74_08590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK49642.1"
                     /translation="MGGESMPGRRIWSYHEPSVHEAASNIIRRHSTNPRDVREQALDH
                     RDLSGVRSALDLGCGFGYMAEEVARRSHPAATIIGVDAHAANWRPFVRRVRSAGRGAR
                     FRRMRIRSVLPWADGSFDLVVCSYSLYFFPEALPEIGRVLKETGRLLAVTHSEESVRD
                     MLVLVGAAQERSALFTLVERFSAESGSAALSAQFKIVERVDYPNTLRFDQGDLDDLLT
                     YLRFKFRSMTDWPESEPEMHRLHVRDLEESLSRPGPIFLRKDDAAFWASGRRAPVPPT
                     "
     gene            complement(7908..>8568)
                     /locus_tag="VFD74_08595"
     CDS             complement(7908..>8568)
                     /locus_tag="VFD74_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK49643.1"
                     /translation="LGVTGYHLLLNFAEVTVTAGAASLLVAAVPIFAALLAKFYLRER
                     LSLWGWLGILVSFSGVAVVALGEGEGMSFDPRAVLVLLAAVCGAHYFVLQKPLLSRYG
                     ALRLTAYCIWAGTIPFLVFFPGMTRSLGGAPVAATVAVAYLGVFPAAIAYLTWNFALS
                     RAPASVVASFLYLPPVLAIFIAWVWIGELPTLLTVFGGAVAVVGVAIVNTRGRARVVA
                     S"
BASE COUNT         1538 a         2961 c         2789 g         1280 t
ORIGIN      
        1 cgccgatgcc gtcgcccgtt ggaccatctg ctcaagatcc cccctcaccg gcttctgggg
       61 tgacgccaac gtgggaggat acttcggcgc cgaactcaag tccgccggct atgacggcgt
      121 ggtgatcacc ggagcggccg ccgcccccgt gtacgtggcc atccacgacg gcgaggtcga
      181 gatccgcgac gccgccgact actggggcgc cgacgtgtac gacaccaccg accggctgac
      241 cggagacctg cgtcgcgaag gagggcgggc cggccaggtg ctcgccatcg ggccggccgg
      301 ggaacgactc gtgcgctatg ccgccatcgc gaaccgtaag cgccactggg ccggacgcgc
      361 agggctcggc gctgtgtggg gggcgaagcg cctcaaggcg atatatgtgc gcggcacggg
      421 gtcggtgccg atcgcccacc cggagcgctt ctcggcgctg cgggccgaac tcaaggcact
      481 ctacgccgac gaccttctca tcgaagcatt gcgggcgttc ggcaccatcg cctccatcga
      541 cctcggaacc atgaccggcg acgtccccta tcagaactgg cgttcgggcc actggaacgg
      601 gatcgacgag ctctcgtcgg tggcctacca ggagcggatc tacagcggga gcgccgggtg
      661 ccaccggtgc ggcgtgcgct gcaagcgcga agcgcgcgtc acggagggcc cctacgcggg
      721 cgtcgacggg cccggtccgg agtatgagac gctagcagtc ttcggtccgc tgtgtctgaa
      781 ctccaacctc gaagccatcg ccaaggcgaa cgaactctgc aaccggctgg gcctcgacac
      841 catgacgtgc ggctcgacca tcgccttcgc gttggagtgc atcgagaacg gactcctcga
      901 cgcagcggaa tggcccggac cgaccgccga ctggggcgac cacgagggca tagtggctct
      961 ggtggagcag atcggtcgcc gcgaggggct cggagacctg ctcgcggagg gcagcgcccg
     1021 ggtcgcggca cggctcggag gccgggcgtt cgaattcgtc tcgacggtga agggtctcga
     1081 agcgcccatg cacgaccccc gctcggccca tggactgggc ttggcatatg gcgtctcggc
     1141 gcggggcgcc tgccacaacg ccagcctcca atacccgatc gaacccggcg ggatgttcgt
     1201 cgaagacgtg cccgacctag ccgtggagct acccgaacag agcagcgtgg gcaaggcggc
     1261 catgaacgtg gccacccagg actacggcgt cttcttcgcc ggttgcgcgg ggttctgcaa
     1321 cctcggcgcc cgacctctgt cggcggcgca ggcggtggag atgatcgact cagtcaccgg
     1381 cgcggggtac acgctcgagg agctcttgac cgtcggacgt cgcgtgtggc tcttgacgag
     1441 gggtttgagc aacctcttcg gggcgcgcgc cgcagacgac aacctgccgc cacgcctgtt
     1501 gcaggcgctc gacgaggggc ccacagcggg cggctcgccc gacatggacc tcatgctgag
     1561 cgagttctac aagctccgcg gcctcgatga gagggggatc ccccgacggg acgtcctcga
     1621 ggcggtcgga ctccgcgatc tcgccgacct gctggagtga gcttgccgcg ccgcccgtcg
     1681 gtggcgcact cctcagcccg tcggcgcggc cgcccgctac tgcagctgga agaacgtggc
     1741 gacgctgtcc gtcgccagcg ggatgaacgg gcgctccgtc atcgaccgga tggacacaaa
     1801 gcagtccttg cacgtgtggc gcacgtcgaa cccacgcacc gccgcctcgc gggtcgggta
     1861 gacgagatcg gggtgcatcg agcacgggtt gatgagcccg tcaggccgga tcacaaggaa
     1921 gcgcagcccc gccatacaac cgcccctgcc gccgctcttg aagaagtcga gcgtggtagc
     1981 gaggacgtag tcggagttca acaccgaccc ggttgcgcga cgtcgctccc tgaggtatct
     2041 gaagtggtcc gcgagcactt cgaggtcctc ggggtcgctg atgctgtagc ctcggtcgcc
     2101 ggtgcgcagg tggctgtagg ccgagaagga gatcttggtg ccccactgct ccgcgcgctc
     2161 gaccagcccg gggatctcgc ggaagttggc gctcgtgagt gccgtgttca ggacgatggc
     2221 gtcgccgccg ggtaggcgag tcagcttcgg gacggtctcg tccagacgag cgaagagccc
     2281 tcgcagttgt cggaaggagt cgtgacgctc gtccgggaaa tcgagcgaga tcgagaacac
     2341 atcaacgccc gcgttcttca gcgaccggaa cctctcttcc gtgagcgccc atccgttcga
     2401 gacgcacacg atcaagggga gtccactcca tcgtcggatg gcgcgggcga tctcctcgag
     2461 gtcgggccgg gtcagaggtt ctcctccgct caattgcgtc acggcagggc gcaactggcg
     2521 cgaccagcgg gcgtagtcct ccggctgcag cagcccctcc tcccgcttca ggcgaccctt
     2581 gtcgcaatgc agacagtcgg cggtgcacga gtgcgtcacc tcgaaggaca cgcagatcgg
     2641 ccaccgtttg acccggttca caaggctccg agcgcccacc ttgaccatca gttggcgatt
     2701 caaacctgct gcctcctcat cctcagtctt ccacgaatag ccgggagtcc gtgatcggcc
     2761 acctgcttga gcacacctat gagtagcacc gctaccagta tcgaaagcag cagccacgcc
     2821 ggcagtctca gcgtctctcc cagccaggcg atgagcagat accgaggcgc ccgcgcgacg
     2881 gtgagagcgg tcaagtaccg cctcatcgga taccgcaccg agaacgccat catcttgaat
     2941 gggaagaacg gtatcggcga agccgcggtc agcacgagaa tgaagaacgg cgcacgctgg
     3001 aaacgcgcga gcaagccgcg gacccagcgc ctctccacat acccgccgag tctatcccac
     3061 ccgaacaacg gcacgaagat acgccagtcg agatacccgg cgatcaccgt gccgacagtc
     3121 gccgagagcg tggtgatcag aagaggctgg tgggctccgt agtcgagaag ggcgacctcg
     3181 tgagagaaca acgagagtcc ggtgttcgcg gggatcgagt acagcagtag aagcagctgg
     3241 gggtggatcg gcgaggctcc gccggaagcc cgcagcgcga cggtggcgag gcccagagcg
     3301 atcgtgacga tggtgaacac caccgcccga gtgcccggtg accccgtgct ccgtccagcg
     3361 aacatcgaac ccccccgtac tgcctgacga ctcccgcttc acagtagcag gaagaaggct
     3421 cgatacaagc gcaggcggat gtgcgagtcg aaggggctac ccgaagaacg tgcgggctat
     3481 gtcgtagagt tcccgcggca ctcgcttgag gtctgcaagc gcctcgctca gcggcacgga
     3541 gatcaccgcg tcgccgcgca gagccgccat catgccccac tcacccgcct tgaccatctc
     3601 ggccgccttg actccgtagc gcgtcgcgag cactctgtcg cgcgcggtgg gcgtgccgcc
     3661 ccgctggatg tatcccagga tggtcacgcg cgcctctatc ccggtgatgc gctcgatctc
     3721 gcgggccagg accgtgccca ctccacccag gcgaacgtgg ccgaaggcgt cgacctcgtc
     3781 ggagctcacc accgtctgcg cggtgccgct cccgctgaga agggagatgc cctcgctcac
     3841 aaccacgatg gagaagcgct tgccgcggtc cgcccgttgc tgcaccaacc cgcacacttc
     3901 ttccaccgtc atggggaact ccggtatcag gatggcgtcg gcgccccctg cgatgcccgc
     3961 catcacggcg atccaaccgg tctgccgtcc catgacctcc accaccatca cccggttgtg
     4021 ggattcggcg gtggagtgca ggcggtcgac agcttcggtg gccacgtgca cggcggtgtc
     4081 gaagccgaac gtgtagtcgg tgcccgagag gtcgttgtcg atggtcttcg ggaccccgac
     4141 caccttgagc ccatactcat catgaagccg caaggccacg cccagtgtat cttcgccgcc
     4201 gatgaccacg acggcttcga tggcgtgggc gcgaacggtc tccagcaccg cctcgaccct
     4261 gccctcccgc ttgaacggat tggtgcggga cgtgcccagg atggtgcctc cctcatgaag
     4321 gatgccgctg atcgacccga gttcgagccg ctgcacgtcc ccgtcgacca ggccaagcca
     4381 cccgttgcgt attccgaaga cctcccaccc gtacgaggcg atggcgcgcc gagtcgcggc
     4441 gcggatcacg gcgttcaacc caggacagtc gcctccacct gtgagcatag cgatacgcat
     4501 gatctgctct cccttccgca gctcgacgcc gcgcctcaat cgactccatg actccatatg
     4561 ctgatgacgg gcgtcaccgg cggcgcgaca cctctgcccg gagtcgctcc gacagcgacg
     4621 cagaaagacc cagccggtcg atctcccact ccaactgctc gatcgtgcca tacccgtcga
     4681 gatacccatt cacccgcgcg gcggaggtgg agtcgacgag atcggcctcc accgggtaga
     4741 actcctcgaa ctccgccgcg aagggcccgg aaccacgtag gcgatacttc tggtggtagc
     4801 cctccgcgcg atagaagcgg cctgccggga gcacctcggt atgcagttcg gcgccgagcg
     4861 cccgggccat gcgctcgcgc gtgcgctcgg ccgcgacgcg ctgctcggcg tcgtgtacga
     4921 agaccgcgtt cttgtactgc accgaccatg cggcgcgccg agggtcgtgc gaagcccaga
     4981 acgagtagag caactcttcg tacgagacgc gttccgggtc gaagtcgatc tggatggtct
     5041 cgctgtggtc gcccagtcga tgataggtgg gggacggagc ggagcctccg gagtacccga
     5101 cccgcgtgcg cacgactccc tgcaacgccc cgaaccgggc gtccggaccc cagaatcagc
     5161 cgagggcgaa cgtcgccgtg gagagcaggc ctgggatctc acggtcgatc ggcggaatgc
     5221 gacacacctc cctcgccctc tccgagcccg cctcctcagc gcgtggcgcg ctcatcgcgc
     5281 ctctcctcgg tcccgcggcc ggaactccag agccaacgag ttcaggcaat agcggacgcc
     5341 ggtcggcgga ggtccgtcgg ggaagacgtg gccaacgtga gagccgcagc ggctgcagac
     5401 cacctcagtg cgcggttcga acaggccgac gtcaacctgc tcctcgacgc gctccggctc
     5461 gatcggctca tagaaactgg gccatccgga acccgagtcg tacttcgcca gggaatcgaa
     5521 caactcgctg ccgcacgcga cgcaatggta gatgccgtcg cccttgaagt cgtgataggc
     5581 gccggtgaac gccggctcgg tgcctttgca gcgtgcgacc ctgaattggt ccggagtcag
     5641 cgtgcgccgc cactcgtcgt cggtcttcgt caccgctcgt cgccccatgg tatgcagccc
     5701 tcctgacggt atggatgcgc cgcggcacac acggtatcgg cgctcgcggc ggctatcgac
     5761 tctccctcgg taggtattct gccctgcgct cctcggaacc aacctagcgc gacaaggagg
     5821 gcgcgactcg gcgcaacctg gggcgcaacc tggggcgcag cctggggcgc agcctggggc
     5881 gcagcctggg gcgccgctcg agacgccgcc tgcggtgcag ctcgaggcgc gcgtcacttc
     5941 gacgagtctc gcacgtcttc gaagccccgc gctgcgtgat actgagccct gtcgaagaac
     6001 gcccctatcc gactcatgag ttccaccacc cggtagagat cggccgggcg ctcccagacc
     6061 cccacctcgt gcgcgaagtc atacacgctg cgcttgagaa gcgagagcga gctcaacacc
     6121 tcgtgtgccg cacagccggt ccgagcgcgt tcggcgcccc aggtgcggta gaaggcccgc
     6181 acctcggcgg cgccccacga catgaccccc ggcgcctgcg cctcgggttc cactctcagc
     6241 caagacccga gcctcgacag ggctgtggtg gcctccttct ccaggaggtc gggagcccgg
     6301 ccgcgcaggc agacggtagt cggatgcgcg agcatctctc gcgcccagcg ccgggcgatc
     6361 tccgacgcgt gctgctcgat catctccacc aggggatcgg agagcagttc cccccgcctc
     6421 tcgcgcagat ggtggaagga gtcctggatg cgccactcct cctcgtgccg cgccgcgcat
     6481 gcctcgatcg cccgatcgtt ggaggggaac gccagcggcg gcagatcgtc gagcgagaag
     6541 tagcccacat ccacggcgtc gtcgccggcg acttcccggc ctccggtctt ctccacctcg
     6601 aaactgacga tgagcaggtc gccatagtga ctgctctcaa aggagtccac gtcgaggaga
     6661 atccgcacct gcccctcgat gcccgcctcc tcgcgcagtt cccgcagcgc ggcatcttgg
     6721 atggtctcgc cgatctccgc aaacccgatc gggaggcacc acatgccctc gtggggctcc
     6781 tgccctctgc gaacgagcag cacccggcgc tcgtggtcga ggaccaccga ggcgaccacc
     6841 gggagcgggt tcacatagaa gacggtcccg cagcggggac agacctcgcg gtcacggccg
     6901 tcttcgccgc gaaccaccac ttcttccccg cactgagcgc agaaccgagc cttcatcgcg
     6961 accactccct acgttggtgg aacgggcgca cgccgaccgg aagcccagaa ggcagcatcg
     7021 tccttgcgca agaagatagg cccgggacga gacaagctct cctcgaggtc tcgcacgtgc
     7081 agacgatgca tctcgggttc cgactcaggc cagtccgtca tggaccggaa cttgaatcgc
     7141 aggtaggtga gcaggtcgtc aaggtcaccc tggtcgaacc gcaacgtgtt cggatagtcc
     7201 actcgctcga cgatcttgaa ctgagcggag agcgccgccg accctgactc cgccgagaac
     7261 ctctcgacga gcgtgaaaag cgccgatcgc tcttgcgccg cgccgaccaa gacgagcatg
     7321 tcgcggacgc tctcttcgct gtgtgtgacc gccaacagcc ggccggtctc tttcagcact
     7381 cggccgatct ccggaagcgc ttccggaaag aagtacaagg agtagctgca gaccaccaag
     7441 tcgaaagagc catcggccca gggaaggacg gacctgatcc gcattcggcg gaaacgcgcc
     7501 ccccgcccgg cggagcgcac gcggcgcacg aacggccgcc aattcgcggc gtgcgcgtcc
     7561 accccgatga tggtcgccgc gggatgcgaa cgacgggcca cctcctccgc catatagccg
     7621 aagccgcacc ccagatcgag cgccgacctc actccggaga ggtcgcggtg gtcgagggcc
     7681 tgctcgcgca cgtcgcgcgg gttggtcgag tgccgccgga tgatattcga agcggcttcg
     7741 tgcactgacg gctcgtggta cgaccatatg cgccgaccgg gcatggactc cccccccatt
     7801 cgggatcctc gtgccaagga taactccatc gggactccga cgcacgagcc gcgcggacta
     7861 cgcaaggcgc cggccgagca cggatgccgg cgggtcgatc ctcggtgtca ggaggcgacg
     7921 acccgcgcgc gaccacgggt gttgacgatg gcgaccccga cgaccgccac ggcacccccg
     7981 aacacggtga ggagcgtggg cagttccccg atccagaccc acgcgatgaa gatcgcgagg
     8041 accggtggca ggtaaaggaa gctggcgacc accgaagcgg gagcgcggga cagggcgaag
     8101 ttccaggtga ggtaggcgat ggccgccggg aaaactccga gatacgcgac ggccacggtc
     8161 gccgcaaccg gagctccgcc caggctgcgg gtcatgccgg ggaagaacac caagaatggg
     8221 atggtgcccg cccagatgca gtacgcggtc aaccggagcg ccccgtaccg actcagcaac
     8281 ggcttctgca ggacgaagta gtgagcgcca cacacggccg cgaggagcac caagaccgca
     8341 cgagggtcga aactcatgcc ctccccctcg ccgagggcca cgacggccac gccggagaag
     8401 ctcaccagta tgcccagcca cccccataga ctcaagcgct cacgcagata gaacttggcg
     8461 aggagtgcgg cgaagatggg gacggcggcg accagcagac tggccgcgcc cgccgtgacg
     8521 gtgacctctg cgaagttgag tagcaggtga tagcccgtga cgcccaag
//
LOCUS       DASRMJ010000312         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_279057, whole genome shotgun sequence.
ACCESSION   DASRMJ010000312 DASRMJ010000000
VERSION     DASRMJ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279057"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..590)
                     /locus_tag="VFD74_08600"
     CDS             complement(117..590)
                     /locus_tag="VFD74_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49644.1"
                     /translation="MSTEGHHTHPNYSSGEDYSLEFRSFRFSFNSVDFAQRVETAAAE
                     LDLVESGSLDDDERTDLVQLASSGSLDFPVSALGEHLVGLGDEALMHKNECLIYWLRE
                     LVFRGAWLDQRLLEDQLDVRFDEERGCFTYHPRGQRSREIGPPPHPTWRDVAYRK"
     gene            739..1458
                     /locus_tag="VFD74_08605"
     CDS             739..1458
                     /locus_tag="VFD74_08605"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HZK49645.1"
                     /translation="MHRPNSLTTLSALALILLVIVMASSAVGCSSGGDDAVDPVQAFA
                     DSAEAMKAMQSFHFTYEVTKPKDAPASDGVEIVRIVGDVIADGNMQATIDLLQKGVPL
                     QVAFVAAGATHYIQDPTSQKWQSVPAAFSPVGSLNLNKGTIQVLERIGESEYVGTEDL
                     GGTNTYHLKGVVQAADVAAIAGSTTASDPFEGEVWIGVDDHFVRRIVIAGAATANEVD
                     GTIRTIELSAFDEPLEIPPPQ"
     gene            1479..>2018
                     /locus_tag="VFD74_08610"
     CDS             1479..>2018
                     /locus_tag="VFD74_08610"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK49646.1"
                     /translation="MPTHPAPEPPAPLEATSVISRHRTTLALAVLCAAVFLAALDQTM
                     VVTVLPAIMRTLHIPYTKLNDAAWIVTGYLLGYTVAMPLFGRLADVRGRRLMFMAALG
                     VFALGSVLCVLAGELRWLVAARVIQAAGGGALVPVAMVVAVGLLPERRAFALGLVGAS
                     AEAGGVLGPLYGATLSQIWG"
BASE COUNT          360 a          686 c          638 g          334 t
ORIGIN      
        1 gagacagggg tagcgatggc gtcggaacag agttccaggt agtagcagat gaagacgcgc
       61 ttcaggcggg cacggagctg cgaggtcgaa cggtaagcac tgatcatgac tcccggctac
      121 ttgcggtagg ctacgtcgcg ccaggtaggg tgaggaggcg ggccgatctc acgcgagcgt
      181 tgcccgcgtg ggtggtaggt gaagcaacct cgctcctcgt cgaaccggac atcgagctga
      241 tcctcgagta gacgctggtc gagccacgcc ccccggaaca ccagttccct cagccaatag
      301 atcaggcact cgttcttgtg catgagggct tcgtccccga gaccgacgag atgttcccct
      361 aacgcgctca cagggaagtc cagggagccg gaagaagcca gttgcacgag gtcggtgcgc
      421 tcgtcgtcgt cgaggctccc ggattccacg agatcgagct ccgccgccgc cgtctcgacg
      481 cgctgagcga agtcaacgga attgaagctg aagcgaaaac tgcggaactc aagggagtag
      541 tcttcgccgg agctgtagtt cggatgcgta tgatgccctt cagtggacac tcccaccccc
      601 aaggccggtc atccggtcga cggccccatt ctaccaaaca cgctcgatcg gccaaacgct
      661 ttcgccgact ccgcacgcgc agagctttcc gcacgcgcag agcttgccta tgccgacctc
      721 ccgatacata atagccccat gcaccgtccg aactccctca ccacactcag cgccctggca
      781 ctcatcctcc tggtgatcgt gatggcctca tcggctgtcg gctgctcaag tggcggcgac
      841 gacgccgtcg atccggtgca ggcattcgcc gactcggccg aagcgatgaa ggcgatgcag
      901 agcttccact tcacctacga ggtgaccaaa cccaaggacg cacccgcatc cgatggcgtg
      961 gagatcgtcc gcatcgtggg cgacgtcatc gccgacggca acatgcaggc caccatcgac
     1021 ctacttcaga agggcgtccc actccaggtc gccttcgtgg ccgcgggcgc cacccactat
     1081 atccaggacc cgaccagtca gaagtggcaa agcgtgccgg ctgccttcag cccggtggga
     1141 tccctcaacc tgaacaaggg cacgatccaa gtgctcgaac gcatcgggga gtccgaatac
     1201 gtgggcaccg aagacctggg gggcacgaac acctaccatc tcaagggcgt cgtccaagct
     1261 gccgacgtgg cggccatcgc cggttccacc accgcgagcg accccttcga gggtgaagtg
     1321 tggatcggag tcgacgacca cttcgtgcga cgcatcgtca tcgcgggcgc ggcgacggcc
     1381 aacgaggtcg acggaaccat ccgcaccatc gaactctcgg cgttcgacga acccctggag
     1441 atccctcccc cgcagtagcg gcggagggac acttcccgat gcccacacac cccgcgcccg
     1501 agcctccggc gccgctggag gcgacgagcg tgatctcccg tcaccgcacg accctcgccc
     1561 ttgccgtact ctgcgccgcc gtcttcctcg ccgcgttgga ccagaccatg gtcgtcaccg
     1621 tgctccccgc gatcatgcgc acgctgcaca tcccctacac gaagctcaac gacgcggcct
     1681 ggatcgtgac gggctatctg ctcgggtaca ccgtggcgat gcctctcttc ggccgcctgg
     1741 ccgacgtgcg cggcaggcgg ctgatgttca tggcggctct gggggtcttc gccctgggga
     1801 gcgtgctctg tgtgctcgcc ggagagttgc gatggctggt cgccgcgcgg gtgatccagg
     1861 cagccggcgg aggcgccctc gtgccggtgg cgatggtggt ggccgtcggc ctgctcccgg
     1921 aacgccgggc cttcgcgctc ggtctcgtgg gagcgtccgc tgaagcgggc ggggtcttgg
     1981 gcccgctgta cggggccacc ctctcgcaga tctggggg
//
LOCUS       DASRMJ010000313         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_172983, whole genome shotgun sequence.
ACCESSION   DASRMJ010000313 DASRMJ010000000
VERSION     DASRMJ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172983"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1786
                     /gene="serA"
                     /locus_tag="VFD74_08615"
     CDS             185..1786
                     /gene="serA"
                     /locus_tag="VFD74_08615"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZK49647.1"
                     /translation="MAGNEKARVLVKEKISAAGIELLREHFEVEVRTDMTDAELKERI
                     NDYDALIVRSATKVTGDMLEASTRMRIIGRAGSGVDNIDVKAATKKGIVVANAPESNA
                     IAAAEHAVALMMAQARQIPQAHVSLKSGKWERSKFGGVELTGKTLGLIGLGRIGMIVA
                     QRAKGLRMDVIAYDPFVSSERFRENGLEQAEHLDALYAKADFITVHLPKMAETMGIVN
                     DAAFAKMKDGVRIINAARGGIIDEDALVRALESGKVASAGLDVFTQEPPPADFPLLAF
                     DNVVATPHLGASTEEAQDKAGTMIAEQVTAGLLGKFVSNAVNIPSVSSEAMETVGPYL
                     PLAEALGKLIMAIVDGPLDEFTVQYDGQIADQDTRLLTVAVIKAMLEGHVADVVNYVN
                     APSIAEERGLKVAEVKDRRLRDFANLITVTARTNKGEFTAAGTTIGPKYRPRLVQIYG
                     HDIDIEPAKHMAFFRYNDIPGMIGRVGTVLGTHGINIAFMNVGRKKTAGAAVMAVALD
                     DAIPQVVLDEIAGIEGFSEARAVAL"
     gene            complement(1903..>2342)
                     /locus_tag="VFD74_08620"
     CDS             complement(1903..>2342)
                     /locus_tag="VFD74_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49648.1"
                     /translation="RQLFVVSHQQRAKETGDVVFGVERNLDGASQVKYRFEPRTRRLE
                     TFRRTWTSDTLRRFPGARVTPAPGLAADAPRAERVMAGGSGSPLQTTLMDIRGPRSGP
                     SSYRPRADGPWEGIWESLGNPDSTGDAGAGPLGGTEPPKKTCC"
BASE COUNT          419 a          748 c          799 g          376 t
ORIGIN      
        1 cagccgcggg tcgaggatga catggtctca cgtgtggggc ggggcgccga gctccgcccc
       61 actggcctgc gtacagggcc ggcactgtac agcccataga ccgcatcgta tgcttgtgtt
      121 cgtggttcaa ggggatactc agggacgcac cgtcgacgag agagatagtg ggagggttcg
      181 agcgatggct gggaacgaga aggcccgcgt tcttgtcaag gagaagatct cggcggcagg
      241 tatcgagctg ctgcgggagc acttcgaagt cgaagtccgc accgacatga ccgacgcaga
      301 gctcaaggag cggatcaacg actacgacgc cctgatcgtg cgctcggcga ccaaggtcac
      361 cggcgacatg ctggaggcgt ccacccggat gcgcatcatc ggccgcgccg gctccggcgt
      421 ggacaacatc gatgtgaagg ccgccaccaa gaagggcatc gtggtcgcca atgcgcctga
      481 atcaaacgcg atagccgcgg ccgaacacgc cgtggccctg atgatggccc aagctcggca
      541 gataccccaa gcccacgtct cgctgaaatc cgggaagtgg gagcgctcga agttcggcgg
      601 ggtggagctc accggcaaga ccctggggct gatcggcctg gggcggatcg gcatgatcgt
      661 ggcacagcgg gctaagggcc tgcgcatgga cgtcatagcc tacgatccgt tcgtctcgtc
      721 cgaacggttc cgcgagaacg gcttggagca ggccgagcac ctcgatgcac tctatgccaa
      781 ggccgacttc atcacggtgc acctacccaa gatggccgag accatgggca tcgtgaacga
      841 cgcggccttc gcgaagatga aggacggcgt gcgcatcatc aacgcggcgc gcggcggcat
      901 catcgacgaa gatgcgctgg tgcgcgcgct ggaatcgggg aaggtagcct cggccggtct
      961 cgacgtgttc acccaggaac cgccgcccgc cgacttcccc ctgctggcgt tcgacaacgt
     1021 cgtcgcgacg ccgcacctgg gagcttccac cgaggaggcg caggacaagg ccggcaccat
     1081 gatcgccgag caggtcacgg ctgggcttct gggcaagttt gtctccaacg cggtcaacat
     1141 cccctcggta tcgtccgagg ccatggagac tgtgggtccc tacctgccac tggccgaggc
     1201 gctcggcaag ctcatcatgg cgatcgtcga cgggccgctg gatgaattca ccgtgcagta
     1261 cgacggtcag atcgccgacc aggacacgcg tcttctcacg gtggccgtga tcaaggccat
     1321 gcttgagggt cacgtggccg acgtggtgaa ctacgtgaac gcaccttcga tagccgagga
     1381 gcgcgggctc aaggtggccg aggtgaagga tcggcgcctg cgcgacttcg ccaacctgat
     1441 caccgtcacc gctcgcacca acaagggcga gttcacggcc gccggcacga cgatcggtcc
     1501 caagtatcgt ccgcggctgg tgcagatcta cggccacgac atcgatatcg aaccggccaa
     1561 gcacatggcg ttcttccgct acaacgacat cccaggcatg atcggccgcg tgggtacggt
     1621 cctcggcacc catggcatca acatcgcttt catgaacgtg ggtcgcaaga agacggccgg
     1681 ggccgccgtg atggccgtcg ctctcgacga tgcgatcccg caggtggtgc tggatgagat
     1741 cgccggcatc gaaggcttct cggaagccag ggccgtggcg ctgtagcaca cgctctgcct
     1801 cgacgcgccg acggctgcga ccgggcgcgc cacgggtaga tgtcgaagta gtgagtcgtt
     1861 ctaaggggcc cccgccgggg gccccttagc gtggtcaacc ggctagcagc acgtcttctt
     1921 cggcggttcc gtgccgccga gcgggccggc gccggcgtcg ccggtgctgt cgggattgcc
     1981 gagtgactcc cagatgcctt cccaggggcc gtccgcgcgc ggacgatagc tgctgggccc
     2041 gctgcgcggg ccgcggatgt ccatcagtgt cgtctgcagc ggcgagccgc ttccgccggc
     2101 catgacccgc tcggctcgag gggcgtcggc cgcgaggccc ggagctggag tcacccgggc
     2161 tccggggaag cggcgcaggg tgtcggaagt ccaggtgcga cggaaggtct caagccgccg
     2221 tgttcgcggt tcgaagcggt acttgacctg actggctccg tccaggttgc gctccactcc
     2281 gaagaccacg tctccggtct ccttggccct ctgctggtga gagaccacga acagctggcg
     2341 gc
//
LOCUS       DASRMJ010000314         6425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_33442, whole genome shotgun sequence.
ACCESSION   DASRMJ010000314 DASRMJ010000000
VERSION     DASRMJ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33442"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1010
                     /locus_tag="VFD74_08625"
     CDS             252..1010
                     /locus_tag="VFD74_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZK49649.1"
                     /translation="MVVLQHADCEPLGTLQPIFDDAFDVVALRLVGDPVAYHHAVETL
                     VKSRAFDGLVALGGPMSVCERERVEGLDDSLRLLKLAVHADVPILGICLGAQLLALTL
                     GAQVRPGAIGGRRKEIGWLPVELTERGRVDPAFHGFGEREPVFHWHGDTYDLPQGGRL
                     LASSSGYPNQAFRWGRWVYGIQFHLEVTPALVLEWTHRYADELAALDYVDAPAMVSRA
                     SVHADGLREKARVLGGHFVECVRESVRQRGEPLR"
     gene            complement(1048..1551)
                     /locus_tag="VFD74_08630"
     CDS             complement(1048..1551)
                     /locus_tag="VFD74_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HZK49650.1"
                     /translation="MSQLTHDLYISVIGAGQATAQEYAVAEEVGRLVAEAGAVLVCGG
                     LGGVMAAAARGAKEAGGRTLGILPGHDRSEANDYLDMVVTTGMGHARNLAVVSSGDAV
                     IAVGGEFGTLSEIGLAAKVHRPVVVLNGWRLQRDEGTPGIWYASTPREAVSLAQQAIK
                     AGSSLSG"
     gene            complement(1548..3863)
                     /locus_tag="VFD74_08635"
     CDS             complement(1548..3863)
                     /locus_tag="VFD74_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK49651.1"
                     /translation="MWRQAGLLELLDRVFGGVRPELFDIFSHDDEPRVECEVVLPARD
                     GRSAELPADLHPYLADALGAQGIRSLWVHQRACWDVARRGGHIIVTTGTASGKSLCFN
                     LPVLDHLHRDPAARALYLYPTKALAHDQIRRLRPLAGGRIEPAVYDGDTSPEARRVAR
                     TRCRILLTNPDMVSMAILPHHERWGDFLFNLTHVVIDEAHTYRGVFGSHVANVLRRLR
                     RAAAFYGAEPQFILASATIGNAVEHAERLTGLPMTPVADDGAPSGRRKVLLWNPAIID
                     EALNLRRGNAREAAEILATLMRAGVRTICFTKSRRSAELVHQYTIEQLADDRGNLGER
                     LSPYRAGYTPEERREIERRLFQGELLAVVSTNALELGIDVGTLDAVITVGYPGTVASL
                     WQQWGRAGRGKGESLALFVAGNDALEQFFIHNPNEMLQRKVEAATIDFTNPYIHDRHL
                     VAAAYEFPLVTDDSEFFGPGLEEAAERATAKGGLRRGFDSWLVAGSGYPAGEISLRSS
                     SPDQFTIVDEETGSVVGSQEAETAFSFLHPGAVYLHRGENYLVTQLDLEGRVAVVRRF
                     FDSYYTQPRKESSTEIVTHELSSPFGPLTMHLGALAVSSRVIAFQKKRLGGDEALGTE
                     ELDLPTQHFLTEGLWFTFPLDILADDKLLPRLPGALHAIEHALIALLPLLAMCDRWDI
                     GGLSTPLHAQTELPTIFVYDGHPGGVGIARQGFSQVEQWFRDAVRLIADCTCESGCPS
                     CVQSPKCGNGNEPLDKELALTLLRAVLSWDS"
     gene            4345..5004
                     /locus_tag="VFD74_08640"
     CDS             4345..5004
                     /locus_tag="VFD74_08640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF018583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HZK49652.1"
                     /translation="MGDPPRYFITCDFDGTVTSEDTLDLLVQRFAPGVWETMESGLLS
                     GELSLTQVMEEEFRRLRVTEREAVEYVLGRARVRDGFAEFVRWAADRGHELVIVSAGI
                     RTLIDPILAAAGLPYLHVHAGDALFTTEGALVCFPPSTAQCIDQCGHCKRDTILAHAP
                     FPGPLVYVGDGYSDFCPTRQADIVFARASLATHLRGEGIFFHPFDDFHTIVSTLEGCG
                     E"
     gene            5095..>6425
                     /locus_tag="VFD74_08645"
     CDS             5095..>6425
                     /locus_tag="VFD74_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK49653.1"
                     /translation="MTWVAMLFLVVALAAALVLLLLFVRERSKRLVAERGRRDPQELT
                     RLRCAVEHMAEMLVVTDRDGLIRYVNPAFERVTGYSKDEVLGVHPRILGSGRPGDAAR
                     GAHKATIESGRVWSGRVTRRKKSGELYEEEATLSPVRDAEGNIVDFVAVGRDITDELV
                     LEEQLLQSQKMEAVGRLAGGVAHDFNNLLTTISGYVSVLSQSLPGDDDRQDDLNEVER
                     AVGRGADLTRRLLAFSRRDVVRPRLVDVGRVVTSTMALLRRAVGDNIELTVDLDPQPV
                     MVVADPGQLEQMLTNLVVNAREAMPAGGTMRVAVRACELGDDEARRLGVAAPGLYAKI
                     VVSDTGIGIPSDVLGHVFEPFFTTKTKSEGNGLGLATVYAIVERAGGVVRVHTTPGRG
                     SEFGVYLPLSTSGGVPAQAAAEDPTSPPPRGSEGVLVVEDEPAVLRLTTRML"
BASE COUNT          991 a         2154 c         2212 g         1068 t
ORIGIN      
        1 caggaagtcg cgcggcagaa catggaagtc cacgtcgttg accgccacga ggtcgtcgta
       61 gcacttgaac agaccccgag cctcgatcag agcgtgcgtg cgggtgggag tcggcatgat
      121 catcatggta ccagtgcggc cgtggaagca cccgccttga cgtcgaggtc cgcggctggt
      181 tgactcggtg gtgccatgga tacctcaccc gagacgccgg cgagatgaac gcccgatcga
      241 cgcggccacg cgtggtggtg ctgcaacacg cggactgcga gccgctcggc accctgcagc
      301 cgatctttga cgacgccttc gacgtggtgg ccctgaggct cgtcggcgac cccgtcgcct
      361 atcaccacgc ggtggagacg ctggtcaaga gccgggcttt cgacggactc gtggctctcg
      421 gcggcccgat gtcggtgtgc gagcgcgagc gggtggaggg cctcgacgac tccctcaggt
      481 tgctcaagct tgccgtgcac gccgacgtcc ccatcctcgg catctgtctc ggcgcccagt
      541 tgttggcgtt gacgctggga gcccaagtgc gcccgggcgc gatcggcggc cggcgaaagg
      601 agatcggttg gctcccggtc gaattgaccg agcgcgggcg cgtggaccct gccttccacg
      661 ggttcggcga gcgcgagccg gtcttccatt ggcacggcga tacctacgac cttccccagg
      721 gaggcagatt gctggcttcc agctccggct acccgaacca ggcgtttcgc tgggggcggt
      781 gggtgtacgg catccagttc cacctggagg tgacgccggc tctggtgctc gagtggacac
      841 atcggtacgc ggacgagttg gcggctctcg actatgtgga cgcccccgcg atggtgtccc
      901 gcgcctcggt ccacgcggac ggcctcagag agaaggctcg ggtcttgggc gggcatttcg
      961 tggagtgcgt gcgggagtcc gtgcgtcagc ggggcgagcc gctccggtga gccaccccag
     1021 tgagtcaccc cggggagtcg tctcgtctca ccctgagagg gacgaccccg ccttgatggc
     1081 ctgctgcgct aacgacaccg cttcgcgggg agtggaggca taccagatgc ccggagtgcc
     1141 ctcgtcgcgt tgcaggcgcc atccgttgag cacgaccacc ggacggtgca ccttcgccgc
     1201 aaggccgatc tccgagagcg tgccgaactc cccgcccacg gcgatgacgg cgtctccact
     1261 gctcacaacc gccaggttgc gggcgtggcc catccccgtg gtcacgacca tgtcgaggta
     1321 gtcgttggcc tcggagcgat cgtgaccggg caggatgcct agggtgcggc caccggcctc
     1381 cttcgctccg cgcgcggccg cggccatcac gcccccgagc ccgccgcaca cgaggacggc
     1441 tccggcctcg gcgaccagac gacccacctc ctcagccacg gcgtactcct gcgcggtcgc
     1501 ttggccggct ccgatgacgc tgatgtagag gtcgtgcgtg agttggctca tgagtcccag
     1561 gatagcaccg cgcgcaggag cgtcagggcg agctccttgt cgagcggctc gttcccgttg
     1621 ccgcacttgg gggactgcac gcacgaaggg caccccgact cgcaggtgca atcggctatg
     1681 agcctcaccg cgtcgcggaa ccactgctcc acctgactga acccctggcg agcgatgccc
     1741 actccgcccg ggtgaccgtc gtacacgaag atggtgggca gttcggtctg agcgtgcaga
     1801 ggggtggaga gcccgccgat gtcccagcga tcgcacatgg ccagcagcgg gagcaacgcg
     1861 atcaatgcgt gctcgatggc gtggagggcg cccggtaggc gcggcagcag cttgtcgtcc
     1921 gccaggatat ccagcgggaa tgtgaaccag agcccttccg tcaagaagtg ctgggtgggg
     1981 aggtcgagct cttcagtgcc gagcgcttcg tcgccgccca gccgcttctt ctggaaggcg
     2041 atcacccgcg aactcaccgc cagcgctccc aggtgcatgg tcagcgggcc gaagggcgag
     2101 ctcagctcgt gggtcacgat ctcggtggac gactccttgc gtggttgcgt gtagtaggag
     2161 tcgaagaagc ggcggacgac ggccacccgc ccttcgaggt cgagctgtgt cacgaggtag
     2221 ttctcgccgc gatggaggta gacggctccc ggatggagga acgagaaggc cgtctccgcc
     2281 tcctggctgc caacgacgct gccggtctct tcgtcgacga tggtgaactg gtcgggagag
     2341 gaggaacgca ggcttatctc gccggccggg tagccgctgc cggccacgag ccaggagtcg
     2401 aagccccgcc gcaggccgcc cttggccgtc gcgcgctccg cggcctcctc caggccgggc
     2461 ccgaagaact ccgagtcgtc cgtcaccagt gggaattcgt aggccgccgc cacaaggtgc
     2521 cgatcgtgga tgtaggggtt ggtgaagtcg atggtcgccg cctccacctt cctctgcagc
     2581 atctcattcg ggttgtggat gaagaactgc tcgagcgcgt cgttgccggc gacgaacagg
     2641 gcgagactct cacccttgcc ccgtccggct cgtccccact gctgccagag gctcgcgacc
     2701 gtgcccggat agcccacggt gatgacggcg tcgagggtgc ccacgtcgat tcccagttcc
     2761 agggcattgg tggacaccac cgccagcagt tctccttgaa acaggcgacg ctcgatctcg
     2821 cgccgttctt cgggggtgta gccggcccga taaggcgaga gtcgttcgcc gaggttgccc
     2881 ctgtcgtccg ccaactgctc gatcgtgtac tggtggacca actccgcact gcgcctggac
     2941 ttggtgaaac agatggtgcg gacccccgcg cgcatgaggg tggcgagtat ctcggctgcc
     3001 tcccgggcat tgccgcggcg caggttgagc gcctcgtcga tgatggccgg attccagagc
     3061 aggaccttgc gcctgcccga gggcgcgccg tcgtcggcca ccggcgtcat ggggagaccg
     3121 gtgaggcgtt ccgcgtgctc gacggcgttg ccgatggtgg cgctggccag gatgaactgg
     3181 ggttcggccc cgtagaaggc cgcggcacgc cgcaggcggc gcagcacgtt ggcgacgtgg
     3241 ctgccgaaca ccccgcggta ggtgtgcgcc tcatcgatca ccacgtgcgt gaggttgaag
     3301 aggaagtccc cccagcgctc gtggtgaggc aggatggcca tgctcaccat gtcggggttg
     3361 gtgaggagga tccggcagcg cgtgcgggcc acgcggcgtg cctcgggtga ggtgtcgccg
     3421 tcgtagacgg ccggctcgat gcgcccgccc gccagcgggc gcaggcgccg gatctggtcg
     3481 tgggccagtg ctttggtggg gtagaggtag agggcccgcg ccgccgggtc gcggtgcagg
     3541 tggtcgagga ccggaaggtt gaagcataga ctcttgccgg aagcggtgcc ggtggtcacg
     3601 atgatgtgcc cgcctcggcg ggcaacgtcc cagcacgccc gttggtgcac ccagaggctc
     3661 cgtatgcctt gcgcgccgag ggcatcggcg agatatgggt gaaggtcggc ggggagctcc
     3721 gccgaccggc cgtcgcgggc gggcagcacg acctcgcact ccacccgagg ctcgtcgtcg
     3781 tggctgaata tgtcgaacaa ctctggcctc actccgccga acaccctatc cagcagctcc
     3841 agcaatcctg cttgtctcca catgtccccc gccttggccg ctcccgtccc aggtccactg
     3901 tagaccacgg atcggacggc acgcgaggcg cctttgggcc cagattgggc gccgctcccg
     3961 gagggtcacc cggactcccg gcgccggacg ccttctgcgg gcaccgccgc cgcagaccgc
     4021 cagccgccgc cccacatcgc cgggcgccgc ggccgcagat cgccaaccgt cgaccgcctt
     4081 ccccgcgccc tcggccgatg ataccccgtg cgaccgcccg gggtgactca gggccatgca
     4141 ggtatacatg cccatagcat gtgggaagag gcgtcgctac acttcacgca tggaatcggg
     4201 cacatcgacc cgtgacggcg acgctcgggt cgcggtgcac gggaccacca gggggaatct
     4261 tcgcggatat cagggtgcgg tcgactccgc acggggatgc tcgcggtccg ggaacggatg
     4321 gagcagtagt atactcgggc cacgatgggc gaccctcctc ggtacttcat cacctgcgac
     4381 ttcgacggca ccgtcaccag cgaggacacc ctcgatctcc tggtccaacg attcgcgccc
     4441 ggagtctggg agaccatgga atccggactc ctctccggcg agctctccct cacccaggtc
     4501 atggaagagg agttccgccg gttgcgcgtc accgaacgag aggcggtcga gtacgtcctc
     4561 ggccgcgccc gggtgcgtga tggtttcgcg gagttcgtgc ggtgggccgc cgaccgaggc
     4621 cacgagttgg tcatcgtcag tgcgggcatc cgcactctca ttgatcccat cctggctgcc
     4681 gccggccttc cctatctcca cgtccacgcc ggcgacgctc tcttcaccac ggaaggcgcg
     4741 ctcgtctgct tcccgccgtc tacggcccag tgcatcgacc agtgcgggca ctgcaagcgg
     4801 gacaccatcc tcgcacacgc cccctttccc ggcccactcg tctacgtggg agacggctac
     4861 tccgacttct gccccacccg ccaagcggac atcgtcttcg cgcgggcttc gctcgcgacc
     4921 catcttcggg gtgaggggat cttcttccat cccttcgacg acttccacac gatcgtctcg
     4981 accctcgaag gctgcgggga gtgaccgccc gcaccgtcgg ctccgcccgc accgtcggct
     5041 ccgcccgtcc cgcacgctcc ggtcttcaac tgccgggtgt tctcgaggca cgcgttgacc
     5101 tgggtcgcga tgctcttcct ggtggtcgcg ctcgcggcgg ctcttgtctt gctgctgctg
     5161 ttcgtgcgcg aacgctccaa gcgtctcgtc gctgaacggg ggcgtcggga cccgcaggag
     5221 ttgacccgcc tgcggtgtgc ggtggagcac atggccgaga tgctcgtggt caccgacagg
     5281 gacggcctga tcagatacgt taatcccgct ttcgagcggg tcaccggcta ctcgaaggac
     5341 gaggtcctgg gggttcatcc acgcatcctg ggtagcggca gaccggggga cgcagcgcgc
     5401 ggcgcacata aggcgaccat cgagagcggc cgcgtctggt ccggtcgcgt gaccaggagg
     5461 aagaagtcgg gggagctcta cgaggaggag gcgaccctct ctccagtcag ggacgcggag
     5521 ggcaacatcg tcgatttcgt cgccgtcggg cgagacatca ccgacgaact ggtgctggag
     5581 gagcaactgc tgcaaagcca gaagatggaa gcggtggggc gtctggccgg cggcgttgca
     5641 cacgacttca acaacctgct caccaccatc agcggatacg tctccgtgct ctctcagagc
     5701 ctgccgggcg atgacgacag gcaggacgat ctgaacgagg tcgagcgcgc cgtcggccgg
     5761 ggcgccgacc tcacgcgccg actcctcgcc ttctcccgcc gagacgtggt gcgaccgcgt
     5821 ctcgtcgacg tcgggcgggt cgtcacgagc acgatggcgt tgctgcgacg ggcggtgggc
     5881 gacaacatcg agctgaccgt cgacctcgac ccccagcccg tcatggtcgt ggccgacccc
     5941 ggtcaactcg aacagatgct caccaacctg gtggtgaatg cgcgtgaggc tatgcccgcc
     6001 ggggggacga tgcgggtggc cgtgcgagcg tgcgagctcg gcgacgacga agcgcgcaga
     6061 ctgggcgtcg ccgctcccgg tctctacgca aagatcgtcg tgagcgacac gggcataggc
     6121 ataccttccg atgtcttggg ccatgtcttc gagccgttct tcaccacgaa gacgaagtcc
     6181 gagggcaacg ggctcggcct ggcgaccgtc tacgcgatcg tggagcgagc cggaggtgtc
     6241 gtccgggtgc acaccactcc cgggcggggc tcggagttcg gagtctatct gccactctcc
     6301 acctcggggg gagtgcccgc gcaggccgcc gcggaggatc cgacgtcgcc ccctccccgg
     6361 gggagtgaag gcgtcctggt ggtcgaggac gagccggctg tgctgcgact cacgacgcgg
     6421 atgct
//
LOCUS       DASRMJ010000315         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_279413, whole genome shotgun sequence.
ACCESSION   DASRMJ010000315 DASRMJ010000000
VERSION     DASRMJ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279413"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..734
                     /locus_tag="VFD74_08650"
     CDS             273..734
                     /locus_tag="VFD74_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZK49654.1"
                     /translation="MDNEPRPPSPITRRDFLVVSLGLMGTLAAAGLLAPIVRYAFPTV
                     HANAATRVRVASTTELTPLGKVVDFEYQETPCTLLQLEDGTYQGLSRVCTHLGCIIKW
                     QPDDTRFFCPCHAGVFSPTGQVLAGPPPSPLPTLKLSLEGEDIWVEGWSSG"
     gene            735..1100
                     /locus_tag="VFD74_08655"
     CDS             735..1100
                     /locus_tag="VFD74_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZK49655.1"
                     /translation="MCHLALLLPIFGIAVFFVLPFPVALVIYLAIVGASVLLYKAVFA
                     AMCRRVVTGREAMIGAEASVKRVKSGRVVVGLRGELWTAQATGFADGAPKQGERVVVT
                     GIRGNTLLVGPLPRILTHS"
     gene            1156..1758
                     /locus_tag="VFD74_08660"
     CDS             1156..1758
                     /locus_tag="VFD74_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49656.1"
                     /translation="MRRSTSGIVMAVGLVVLVAVAWLYLQDRQQVASSSTAATASPGT
                     SDSPGTPASPGASTSLSTSASPSAPSYVARDAGEGGVKVIAAILTPGAVGEDATLADV
                     AAMVDPATEIGFLVTFETHSGDLSNLDLVALSSLSAPNGDHVAVRWVSESDSGHHRAG
                     VLVFERGSLDLAAGTGDLTLTMRDVAGVPSRTLTWLLPLG"
     gene            1769..2506
                     /locus_tag="VFD74_08665"
     CDS             1769..2506
                     /locus_tag="VFD74_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZK49657.1"
                     /translation="MEAPIVLALAAGVVSFLSPCIVPMISVYLTLITGMTMDELTARP
                     GLVIRRTIVINTLLFALGFTIVFTLAGGAAGAIGAALQSATRGLEIVGGVVIILMGLS
                     MAGVFRLRFLDKMRLPLKRPLAKPKGPLGSLVVGLLFAVTCSHCIAPTLLSMMAVAGT
                     TGSPVTGMVTMLAFSLGLTVPYVGTAVAITPALRFLSGHKRAARFVGVGTGVFLAGFG
                     ILLLGGQFTRLVEISSRLLPFRVPLGM"
     gene            2552..>2943
                     /locus_tag="VFD74_08670"
     CDS             2552..>2943
                     /locus_tag="VFD74_08670"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZK49658.1"
                     /translation="MPYRTFEMITLGPLHLRTWGMLVALGILAGAFLAVRIAPSRGIQ
                     ADRLWTLTVVVALAGVLGSKVLWALQPDLIGETIAHPLRLVAIWQGGLTFIGGLLAAV
                     VAGVLYARRAMLPVFDTADVVAPGLALG"
BASE COUNT          432 a          981 c          954 g          576 t
ORIGIN      
        1 tcaggagtgt tcggaacgta ggcggccaga caccgagcgc agcagaagta cacggtcctg
       61 gttccgtcgc gcacggcaca ggtcgcagcg tatggatcga cgaacatccc gcagatgggg
      121 tcgacggccg ggttgtcggg acgcagcggc gcactctttg aagcttctgt aaagatgcgc
      181 ccgcaaggcc gtcgctgccc ttgttccgta tacccccctg gcctatgctg gaagaagaag
      241 caactcccgg cagtcggaaa ggagccggtc tcttggacaa tgaacctcga ccgccttccc
      301 cgatcacccg ccgtgacttc ctcgtagtca gcctgggtct tatgggcacg ttggcggctg
      361 ccgggctcct cgcgccgatc gtccgctacg cattccccac cgtccacgcg aacgccgcga
      421 cccgcgtgcg cgtggcgtcc acgaccgagc tgacccctct ggggaaggtg gtcgatttcg
      481 agtaccagga aactccctgc acgcttctgc aactcgaaga cgggacgtac caggggctct
      541 ctcgggtctg tacgcatctc ggctgcatca tcaagtggca gcccgacgac acccgcttct
      601 tctgtccctg tcatgccgga gtcttctcac ctaccgggca ggtgctcgcc gggcccccgc
      661 ccagccccct gccgacgctc aagctatcgc tcgagggtga ggacatctgg gtcgaaggtt
      721 ggagctccgg ctagatgtgc catctcgcac ttcttctgcc gatcttcggt atcgccgtgt
      781 tcttcgtgct ccccttcccg gtggcgctcg tcatctacct cgcgatcgtg ggggccagcg
      841 tcttgctcta caaggcggtg tttgccgcga tgtgccgccg ggtggtcacg gggcgtgagg
      901 cgatgatagg cgccgaggcc agcgtgaagc gggtcaagtc cggcagggtg gtcgtgggtc
      961 tgcggggcga gctgtggacg gcccaggcca ctgggttcgc ggacggggcg cccaagcagg
     1021 gcgaacgcgt ggtagtcacc ggaatccggg gcaacacgct cctggtgggg ccgctcccac
     1081 gaatccttac gcattcttga cctgattcac tcatactccc ccgaggtatc tggatccgcg
     1141 ggtgttggag atgacatgag aagaagcact tcgggaatag tcatggccgt gggactggtc
     1201 gtgctcgtgg ccgtggcgtg gctgtacttg caggaccgtc agcaggtggc ttccagctcc
     1261 accgcggcga ccgcttcgcc gggcacctcg gattcgccgg gcacaccggc ttcgccgggt
     1321 gcttccacct cactgagtac gtccgcttcg ccgagcgcgc ccagctacgt ggctcgagac
     1381 gccggggagg gtggcgtgaa ggtgatagct gcgatcctga cccccggggc cgtcggggag
     1441 gacgcgacac tcgccgatgt ggccgccatg gtcgatccag ccacggagat cgggttcctg
     1501 gtgacctttg aaacgcactc cggcgatctc tcgaatctcg acctcgttgc cctctcgagc
     1561 ctcagcgccc ccaacggaga ccatgtcgcg gtccgctggg tatcggagag cgactcgggc
     1621 caccatcgcg ccggggtgct ggtgttcgag cgtggaagcc tggatcttgc cgcgggcacc
     1681 ggtgacctca ccctcaccat gagagatgtg gccggggtgc ccagccgcac gctcacctgg
     1741 ctccttccgc tcgggtaggc gggggttagt ggaagcgccc atagtactgg ccctcgcggc
     1801 cggagtcgtc tccttccttt cgccctgcat cgtgccgatg atctccgtct atctgacgct
     1861 gatcacggga atgacgatgg acgaactcac cgccaggccc gggctcgtca tcagacggac
     1921 catcgtaatc aataccctgc tgttcgccct cgggttcacc attgtgttca cgctcgccgg
     1981 aggagctgct ggggcgatcg gggctgccct ccagagcgcc acccgtgggc tcgagatcgt
     2041 cggcggtgtt gtcatcatcc tcatgggcct gtcgatggcc ggtgtgttcc gcctccggtt
     2101 cctcgacaag atgcgacttc cactgaaacg gcctctcgcc aagccgaagg gcccgctggg
     2161 atcgttggtg gtgggcctgc tgttcgcggt cacgtgctcc cactgcatcg caccgaccct
     2221 cctgtcgatg atggcggttg cgggcaccac cggctcgccg gtgacgggca tggtgaccat
     2281 gctcgcgttc tccctggggc tcaccgtccc gtacgtgggc acggcggtgg ccataacgcc
     2341 cgcgctgcgg ttcctctccg gccataagcg ggccgcgagg ttcgtgggcg tagggaccgg
     2401 cgtcttcttg gccggtttcg ggattctcct gctgggcggt caattcaccc gactcgtcga
     2461 gatcagttct cgactcctgc ctttccgcgt tccgctgggc atgtgagcgg cggactccca
     2521 accggcgacc ttcaccagag gagacagcac catgccgtat cgcaccttcg agatgatcac
     2581 ccttggcccc ctccacctgc gcacctgggg catgctcgta gcactgggca ttctcgcggg
     2641 cgccttcctg gcggtgcgca tcgctcccag ccgcgggatc caggccgata gactctggac
     2701 actcacggtc gtggtggccc tggccggggt gctcggcagc aaagtgctct gggctctgca
     2761 gccggatctc atcggggaga ctatcgcgca tcctctgcgg ctagtcgcca tctggcaggg
     2821 ggggctcacg ttcatcggag ggctcctggc cgccgtcgta gccggggtcc tctacgcgcg
     2881 cagagccatg cttccggtgt tcgacactgc cgacgtggtc gcccccggcc ttgcactcgg
     2941 gat
//
LOCUS       DASRMJ010000316         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_248744, whole genome shotgun sequence.
ACCESSION   DASRMJ010000316 DASRMJ010000000
VERSION     DASRMJ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248744"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFD74_08675"
     CDS             <1..228
                     /locus_tag="VFD74_08675"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZK49659.1"
                     /translation="RHMVLFRAHSFQDPAAAHLAARGHRRILERLAARDKEGIAEAVR
                     THLEDARGFIAGRIAEESEIADHAHRADVEE"
     gene            233..1462
                     /locus_tag="VFD74_08680"
     CDS             233..1462
                     /locus_tag="VFD74_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010840771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HZK49660.1"
                     /translation="MTEHDSRTQAAGGAPLTVAVVGGGNGSYTTAADLTLQGHRVRLW
                     PGARERHAAVLESGSIRVSGIPPVGEARLQLVSDDVAAVVSGADVVVCTDPAFTQEDR
                     AARVAPHLSDGQIVFLSPGSLGSYVFHRTVRGLRSRADVTYAEPGTLPYLTRKIGPTD
                     VEISGRAAHLPVGVFPAVRTEETVAILRRIFPGALSTDDILSVALLNVGPIIHSVLVL
                     LNTGAIEHFDSWDIHNEGTSPSVKKVILAHDEERIAVREALGYSGPHYPMRHHYHPQG
                     DEEWMYGRRGHTDLVKSEKWREALSFRHRYIQEDVHVNLALFASIGDLAGVETPIADS
                     LLRLAAAITDQAHITAGRTLTNLGLGAYSRAELQHLLRDGRRFGGGDPSGDIGSAGGG
                     TLAGDADPGGDKDATDA"
     gene            1572..2594
                     /locus_tag="VFD74_08685"
     CDS             1572..2594
                     /locus_tag="VFD74_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZK49661.1"
                     /translation="MRVGVVGPGRMGVGIVTAILMAGGNDTVILADVKDRAPGDEMGS
                     LSRAHMEIRANLDLLNDLGILRGEAGSHLDRLVLTREAVRDLAGCALVFEALPETIEC
                     KRPFYADVLNALGPDCAAASATSTFALDTFRDMGAPAGRVVTAHWLNPAFLMPLVEVA
                     HADWTEPATLDRVLGYLKAIGKIAVPLRSSPGFIVPRIQVAAMNEAVRIVEEGVATAA
                     EVDTAIKAGFGFRLAVLGLMEFIDLGGVDILAHAGSYLSEALGKEHFHPPLSVRDKMQ
                     AGELGPRTGRGYYSYEDVDVAALFRDRYRGFADLLTHVSDSPHLSFYGGVELRGGDHA
                     DQEEHL"
BASE COUNT          449 a          903 c          891 g          402 t
ORIGIN      
        1 cgacacatgg tgctcttccg cgcccacagc ttccaagacc cggcggccgc gcatctggcc
       61 gcgcggggcc accgacgcat cctggaacga ctcgcggcgc gtgacaagga gggcatcgcc
      121 gaagcggtgc gcacccatct cgaggacgcc cgtgggttta tcgccgggcg catcgccgag
      181 gagtccgaaa tcgccgacca cgcacaccgc gcagacgttg aggagtagcg acgtgacgga
      241 acacgacagc aggacccagg cagccggagg ggctcccctc accgtcgcgg tggtcggcgg
      301 aggaaacggc tcgtatacca cggccgccga tctgacgttg cagggccacc gagtgcgcct
      361 ctggcctgga gcgcgagagc ggcacgcagc cgtcctcgag tccggatcca tccgggtctc
      421 gggcataccc cccgtaggcg aggcccgact gcagttggtg tcggacgacg tggcggccgt
      481 cgtgagcggc gccgacgtcg tcgtgtgcac cgaccccgcg ttcactcagg aagaccgtgc
      541 cgctcgggtg gcgccccacc tgtccgacgg gcagatcgtg ttcctctccc ccggttcgct
      601 cggctcctac gtctttcacc gcaccgtccg cggccttagg agtcgcgcgg acgtgacgta
      661 cgccgagccg ggcaccctcc cctatctgac acgcaagatc ggcccgaccg acgttgagat
      721 aagcggtcgc gccgcgcacc tgcctgtggg cgtgttcccg gccgtccgca cggaggagac
      781 ggtcgctatc ctgcgccgga tcttcccggg agcgctgtcc accgacgata tcctgtcggt
      841 cgcgctgctc aacgtgggcc cgatcatcca ctccgtgctt gttctcctca acaccggagc
      901 catcgagcac ttcgacagct gggacatcca caacgaaggc acctcgccct cggtgaagaa
      961 ggtgatcttg gcgcatgacg aagagcggat agccgttcgt gaggccctcg gatactcggg
     1021 cccccactac ccgatgcgac accactacca cccccagggc gatgaggagt ggatgtatgg
     1081 gcgacgcggc cacacggacc tggtgaagag cgagaagtgg cgagaggcgc tcagtttccg
     1141 gcaccgctac atccaagagg acgtgcacgt caacctggca ctgttcgcca gtatcggcga
     1201 cctggccgga gtggagacgc ctatcgccga ctcgctgctc cgccttgcag ccgcgatcac
     1261 cgaccaagcg cacatcacag ccggccgcac cctgaccaac ctggggctgg gcgcctactc
     1321 cagagcggaa ctccagcatc tactgcgcga tggtaggcgg ttcggcggcg gcgacccgag
     1381 cggcgacatc ggctcggccg gcggcggaac cctggccggc gacgcagacc cgggcggcga
     1441 caaggacgct acggatgcct gacgccgtcc catcgagccc cacaggtggc gcattcggcc
     1501 ccgcagatgc ctctgccccc ggcggcacgg acctcggcgg cccaaccgct cccgtgagcg
     1561 gggcgcggcc catgcgagtt ggtgtcgtgg ggcccggccg catgggcgtg ggcatcgtca
     1621 ccgccatcct gatggcgggc gggaacgaca cggtaatcct cgccgacgtg aaggaccggg
     1681 cgccgggaga cgagatgggt tcgctgtccc gagcccacat ggagatccgc gccaacctcg
     1741 acttgctcaa cgacctcggc atacttcgcg gggaggcagg ctcgcatctg gacaggcttg
     1801 tgctgaccag ggaggcggtg cgcgacctgg ccgggtgcgc cctcgtgttc gaggcgctgc
     1861 ccgagacgat cgagtgcaag cgcccgttct acgcagatgt tctgaacgcc ctgggccccg
     1921 actgcgcggc cgcttcggca acctcgacgt tcgcgctcga cacctttcga gacatggggg
     1981 ccccggccgg tcgggttgtc acggcgcact ggctcaaccc ggccttcctt atgccgctcg
     2041 tcgaggtggc acacgccgat tggaccgagc ccgcgacgct cgaccgcgtt ctcggctatc
     2101 tcaaggccat cggcaagatc gccgtgccgc tgcggagcag ccccggcttc atcgtgccgc
     2161 gcatccaggt ggccgccatg aatgaggcgg tgcgcatcgt cgaggagggt gtggccaccg
     2221 ccgccgaagt cgataccgcg atcaaggcgg ggttcggatt ccggctcgcg gtgttgggac
     2281 tgatggagtt catcgatcta ggcggggtcg atatcctggc tcatgcgggc tcctacttgt
     2341 cggaagccct tgggaaggaa cacttccacc ccccactctc ggtgcgcgat aagatgcagg
     2401 cgggagaact cggaccgcgc accggcaggg ggtactacag ctacgaagac gtggatgtag
     2461 ccgcgctctt cagggatcga taccgcggat tcgccgacct gctcacccac gtctccgact
     2521 cgccgcacct gagcttctac ggaggggtcg agttgagagg cggggaccac gccgaccagg
     2581 aggaacatct atgacgagtg gacgacggga cgggatgggg cgggatgagg cggacagaac
     2641 ggaaa
//
LOCUS       DASRMJ010000317         7980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_70160, whole genome shotgun sequence.
ACCESSION   DASRMJ010000317 DASRMJ010000000
VERSION     DASRMJ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70160"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFD74_08690"
     CDS             complement(<1..654)
                     /locus_tag="VFD74_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZK49662.1"
                     /translation="MQSDSDPQGGSHIFESDPTHRDDPGHVDAWHESALDLIAGTLAP
                     HVAERLEAHLAACPECRETLADQRSIASILNSIAEAAVPAELDDSVLAGLAALAQPSP
                     VAAARATSSRIGTEGGALRSLRRFLVPRVWLPAAAMALVAGVAISSYYQVGIGSNGDA
                     DRLAAESQQKDVSAASPEAASADDAATQAAESAAGGQTTLAGAPPTTASAASGPMYAA
                     "
     gene            complement(644..1351)
                     /locus_tag="VFD74_08695"
     CDS             complement(644..1351)
                     /locus_tag="VFD74_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK49663.1"
                     /translation="MPLPLTPDPDAELVARSRAGDQAAVTELYRRHSQRGYNLALRMT
                     GDPWDAADVAQEAFIKAFSHLSSFKGEARFSTWLHRIVVNCVHDHLRRRRPDPMEDVM
                     LEHLSAPSSRGGSSLTVASVSPAEDGLSTEVKSALAALPEGFRLAIVLCDLLGYGYAE
                     AAEILEVQEGTIKSRVFRARAELAESLRGAGFYPGGHGNHGPESAVSPADPTHQDPDL
                     SSRPRPHDRREKRADAV"
     gene            1482..2624
                     /locus_tag="VFD74_08700"
     CDS             1482..2624
                     /locus_tag="VFD74_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HZK49664.1"
                     /translation="MFEDVGRIFEESRDLTIGIEEEFQILDGSTLALVNRFADLKTAA
                     DALFGAPLVTGELIQSEVEINSAVCETFSDAQADVALRRAALVSGAHAIGLALCSTGV
                     HPFSRWEDQRFIESPHYLRVVESLRYVAWTNNTFGMHVHVGVRGADRAVALCDAFRSL
                     LPPLLALSASSPVFRGKETGLHSTRAQVFIRSFPRCGIPDAYGDWAGYADYAQFLYDT
                     GSVIEPTQIWWTVRTHHTYGTLEVRIADAQPRLDESMALAGLIVGLAAALMERFDREG
                     RLPVHAGRFIEENRWRALRDGLDGRLIDLDTRTEIPAVDVVRSLVAEVEPLAGKLGLE
                     GALAVVTRMVDEGNSAQRQLRMLHDGVSVEEIHRLTVRETMGGIGP"
     gene            complement(2637..3638)
                     /locus_tag="VFD74_08705"
     CDS             complement(2637..3638)
                     /locus_tag="VFD74_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49665.1"
                     /translation="MDYSDLLRRAWRVAWRHKYLWLLGLVASEGGCGISGGAPNISYS
                     FGSDQGYGPFASGSLDLADWIAHNWPFLLALIVGLFLLGIVLWVISIIATGGLIAGSD
                     AANAEGPSGLGFAWSAGVRSFWRLLGMWLLVAVVALAIGLLFALAIGLPLGLALARGA
                     HLGTGVIVALAVTALLLLLLAIPLAIVVQVVLTWSSRSLVLEGRGPVGSIRNGWRVFR
                     ANVGASFMTWLVSVGVSIAAGIALVLPLALLAVPLGLLVYRTATDGADGVMWGSIALV
                     GLVALLVLAIFKAVFTSFSTAYWTIAYVNLASVSEPAASLQPAEPPLSEGTLPDPPL"
     gene            3739..4158
                     /locus_tag="VFD74_08710"
     CDS             3739..4158
                     /locus_tag="VFD74_08710"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HZK49666.1"
                     /translation="MVDERAPATESELMEQLEEEMRRITVPDYLRHLLVSLSSLGFQR
                     LGLTAESAAERDLVQSRMAIDAFEALVGVLAPALSKEDESLFRSALHQMRMAFVRASA
                     GESAGPEGVEEDEAAQGGEKTEAGAEAAGEGDNGGDS"
     gene            4166..4942
                     /locus_tag="VFD74_08715"
     CDS             4166..4942
                     /locus_tag="VFD74_08715"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018737109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZK49667.1"
                     /translation="MFGGSGFYSFLDDVEEVPVETPYGPPSDSFFIGEVGGKRVAFLP
                     RHGRRHQLPPHMINHRANVWGMKSLGVDRIIGPCASGSLKREVGLGHFVVCDQFVDRT
                     TGRKDTFYDGPVSTHVSAADPYCPTIREALVRKGRELGITMHDGGTVVTIQGPRFSTR
                     AESRWFASAGWEVINMTAYPEGYLARELEICYANVSLVTDYDVGVADDPSVAAVSHAD
                     VVAVFEQNNARLRELLFAVIPALGGRRDCVCASALASARL"
     gene            complement(5021..5365)
                     /locus_tag="VFD74_08720"
     CDS             complement(5021..5365)
                     /locus_tag="VFD74_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49668.1"
                     /translation="MARRERRDGRGESRDDYDSQPDRNGAPHEPERETLTALKAILVK
                     VASVVKPLGLTPEESTDLVQRMYGMVLELDAAMAGETDESRKASMVAHVKSSVIRRED
                     DVLVVDHPAKAD"
     gene            complement(5368..6297)
                     /locus_tag="VFD74_08725"
     CDS             complement(5368..6297)
                     /locus_tag="VFD74_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZK49669.1"
                     /translation="MRVLVTGGAGFIASHVSDRLLDRGFDVAIVDNLATGKRENIPAG
                     ARFHEVDVRDARLAEVFEAERPDIVIHHAAQMDVRKSVTDPRYDADVNLLGTLNLLEC
                     CRVHGTRKVVYAGTGGAMFGEAAYLPVDEKHPVQPISPYGVSKHTVEHYLYAYRANFG
                     LDYTVLRYPNVYGPRQDPHGEAGVVAIFSLQLLNGETPRIFGDGTKTRDYCYVGDIVE
                     ANMLAIERGSDGLFNIGRGIEVSDRQVFESVRDAVGVDIEPEYAPTRPGEVEHIALDA
                     TLARETLGWEWKVDLAEGVGRAVDFYKERRKRV"
     gene            complement(6335..6568)
                     /locus_tag="VFD74_08730"
     CDS             complement(6335..6568)
                     /locus_tag="VFD74_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZK49670.1"
                     /translation="MVRAYVLVKIEAGRAKEILDGLRTIDSVQRADAVTGPVDLIVQV
                     EAEDPRALAELIFRSIQTVSGVKETDTRIVIED"
     gene            6717..6790
                     /locus_tag="VFD74_08735"
     tRNA            6717..6790
                     /locus_tag="VFD74_08735"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6751..6753,aa:Pro,seq:tgg)
     gene            6823..6895
                     /locus_tag="VFD74_08740"
     tRNA            6823..6895
                     /locus_tag="VFD74_08740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6856..6858,aa:Gly,seq:tcc)
     gene            6937..7010
                     /locus_tag="VFD74_08745"
     tRNA            6937..7010
                     /locus_tag="VFD74_08745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6971..6973,aa:Arg,seq:tct)
     gene            7057..7129
                     /locus_tag="VFD74_08750"
     tRNA            7057..7129
                     /locus_tag="VFD74_08750"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7090..7092,aa:His,seq:gtg)
     gene            7256..7338
                     /locus_tag="VFD74_08755"
     tRNA            7256..7338
                     /locus_tag="VFD74_08755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7289..7291,aa:Leu,seq:tag)
     gene            7380..>7980
                     /locus_tag="VFD74_08760"
     CDS             7380..>7980
                     /locus_tag="VFD74_08760"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZK49671.1"
                     /translation="MEGRTELKTTVGEKVGNDVKLEIEVSPEEVQDGIAATVKQLSRE
                     VRIPGFRKGKVPTGMIVQRFGMEAISQQMLDDRLSPWLLEALTEAGLDPVDRPEVDFD
                     EAPEEGKPFSFTAVVTVMPTAELGEYKGVEAPRPDATVLDSEVDERVERLREEFAELK
                     AVGDRPAAEGDFVTVNAEGYRDGEVVANTQLDDYMFEVGG"
BASE COUNT         1329 a         2513 c         2784 g         1354 t
ORIGIN      
        1 cgcggcgtac atcggccctg aggccgcgct cgccgtggtg ggcggtgcgc cggcgagcgt
       61 cgtctgtccg cccgcggcgc tctccgccgc ctgcgtcgcc gcatcgtccg ccgacgcggc
      121 ctcgggcgaa gccgctgaga catccttctg ttgactctct gccgcgagtc ggtcggcgtc
      181 cccgttggaa ccgatcccca cctgatagta ggaggagatc gcgacaccgg cgacgagggc
      241 catcgcggca gcgggaagcc acacgcgagg gaccaggaag cgccgcagcg accgcagggc
      301 tccaccttcc gtgccgatcc ggctcgaggt cgctcgggct gcggccacgg gcgagggctg
      361 cgccagagcg gccaagcccg cgagcacgct gtcgtcgagc tcggccggca cggccgcctc
      421 ggcgatcgag ttcaagatag atgcgatcga gcgctggtct gcgagggtct cgcggcactc
      481 ggggcaggca gccaggtggg cctcgagtcg ttcggcgacg tggggcgcga gggtgccggc
      541 gatcagatcc agcgccgact catgccacgc gtcgacgtgg cccggatcgt cccgatgagt
      601 cgggtccgac tcgaagatgt ggcttccgcc ttggggatcg ctgtcagact gcatcggcgc
      661 gcttctcccg ccggtcgtgt ggtcgcggac gtgatgagag gtcggggtcc tggtgggtcg
      721 ggtcggcggg tgagacggcg ctttcaggtc cgtggttccc gtgtcccccg gggtagaagc
      781 cggcaccgcg cagtgactcc gccaattccg cacgggctcg gaacacgcga gacttgatag
      841 tgccttcctg cacctcgagg atctcggccg cttccgcata gccgtagccg aggaggtcgc
      901 acagcacgat cgcgagtcga aacccttcgg gcagagctgc cagtgccgat ttcacctcgg
      961 tggagagccc atcttccgcg ggcgacaccg atgccacggt cagtgaggac ccaccccgcg
     1021 acgagggagc cgacaggtgc tcgagcataa cgtcttccat gggatcgggc cggcggcggc
     1081 ggagatggtc gtggacgcag ttgacgacaa tccggtgcag ccaggtgctg aagcgcgcct
     1141 cgcccttgaa cgaggagagg tgcgagaagg ccttgatgaa ggcctcctga gccacgtcgg
     1201 cggcgtccca ggggtcgccg gtcatgcgaa gggcaaggtt gtatccccgc tgcgagtgcc
     1261 ttcggtaaag ctcggtcacg gccgcttgat cccccgcgcg cgagcgggcg acgagttcgg
     1321 cgtccgggtc cggagtgagg gggagcggca cctgggatcc tccgattggg atcgttgcgg
     1381 tcctgaggca actggtagac taaagccttc gggggatcca tgcaactgcc cgcccgccag
     1441 ggccggttcg aatgtccgta gcaacggagg tcgccttgcg cgtgttcgag gacgtaggcc
     1501 ggatcttcga ggagagtcgg gatctcacca tcggtatcga ggaggagttc cagatcctcg
     1561 acgggtcgac tctggccctc gtcaatcggt tcgcagatct caagacggcg gccgatgcgc
     1621 tgttcggcgc gccccttgtc accggggaat tgatccagtc agaggtcgag atcaacagcg
     1681 ccgtgtgcga gaccttctcc gacgcgcagg cggacgtcgc gcttcggcgg gccgcgctgg
     1741 tgtcgggcgc ccacgcgatc ggcctcgccc tgtgctcaac tggggtgcat cctttcagcc
     1801 gctgggagga ccagaggttc atcgagtctc cgcactacct gagagtcgtg gagtcactgc
     1861 gctacgtcgc ctggacgaac aacacgttcg gcatgcacgt gcatgtgggg gtgcgtgggg
     1921 ccgaccgggc ggtggccctg tgcgacgctt tccgttcgct gctgcccccc ctgctcgcgc
     1981 tctccgcgag ctctcccgtc ttccggggaa aggagacggg gctccattcc acgcgggccc
     2041 aggtgttcat acggagtttc ccacgttgcg gcatcccaga cgcgtacgga gattgggccg
     2101 ggtatgccga ctacgcgcag ttcctctacg atacgggcag cgtgatcgaa cccacgcaga
     2161 tctggtggac agttcggacg caccacactt acggcacgct cgaggtgcgt atcgcggacg
     2221 ctcagcccag gttggacgag tccatggctc tcgcgggact gatcgtcggc ctggctgcgg
     2281 ccctgatgga gcgtttcgac cgcgagggtc ggcttcccgt gcacgcaggg cgcttcatcg
     2341 aggagaaccg ttggagggcg ctgcgcgacg ggctggacgg tcgcttgatc gacctcgaca
     2401 cgaggacgga gatcccggcc gtggacgtcg tccggtcact cgtcgctgag gtggagcctc
     2461 tcgcagggaa gctgggcctc gagggggcgc tggcagtcgt cacgcgtatg gtcgatgagg
     2521 gcaactcggc tcagcggcaa ttgcgcatgt tgcacgatgg agtgagcgtc gaggagatcc
     2581 atcgtctgac ggtgcgggag acgatgggcg gtatcgggcc gtagtccctc aaagcctcaa
     2641 agcgggggat ctgggagcgt gccctcggag agcggtggct ccgctggttg cagggaggcc
     2701 gccggctcgg agaccgatgc cagattgacg taggcgatcg tccagtaggc cgtgctgaag
     2761 ctcgtgaaca cagccttgaa gatcgcaagc accagcaagg ccaccaggcc aacaagagcg
     2821 atgcttcccc acatcacgcc gtcggcaccg tccgtggcgg tgcggtagac cagcagtccc
     2881 agcgggacgg ccagaagggc cagcggcagc accagcgcga tgccggccgc gatcgacacc
     2941 ccgacactga cgagccacgt catgaagctc gcaccgacgt tcgcgcgaaa cacccgccat
     3001 ccgttgcgaa tcgagccgac aggacccctt ccctccaaca ccagcgaacg gctcgaccat
     3061 gtgagcacca cctggacgac gatcgccaat ggaatcgcca gcaggaggag gaggagagcc
     3121 gtcaccgcga gtgcgacgat gacgcccgtg cccaggtggg cgccgcgggc gagtgcgagc
     3181 ccgagtggga ggccgatcgc caacgcgaac agcaggccga tcgccagcgc gaccaccgcc
     3241 accagcagcc acatccccag cagtcgccag aaactccgca caccggcgct ccacgcgaac
     3301 ccaaggccgg agggcccctc ggcgttcgcg gcatcggagc cggcgatgag tcccccggtg
     3361 gcgatgatcg agatcaccca taggacgatg cccaatagga agagtccgac gatcagggct
     3421 aggaggaagg gccaattgtg ggcgatccaa tcggcgagat cgaggcttcc ggaggcgaag
     3481 gggccgtatc cttggtcgct cccgaagctg taactgatat tcggagcccc gccggagatg
     3541 ccgcagccgc cttcggaggc gacgagcccg agtagccaca agtacttgtg gcgccaggcc
     3601 acccgccaag cgcggcgcaa aaggtcgctg tagtccacga actctcctcc gacgttgacc
     3661 ccgtggtgcc ctgacgggat actatcaccc agcgaaagcg tgaacggccc gcaggagggc
     3721 tgaggaggtg tcgggaatat ggtcgatgag agagcgcccg cgaccgagag cgaattgatg
     3781 gagcaactcg aagaggagat gcgccgcatc acggtgcccg actacctacg gcacctgctt
     3841 gtctcgctgt ccagcttggg cttccagcga ttgggtctca cggccgagag tgccgccgag
     3901 cgggatctgg tgcagtcgcg catggcgatc gatgcgttcg aggcgctggt gggagtgctc
     3961 gcgcccgcgc tttcgaaaga ggatgagtcg ctgttcaggt cggcgttgca ccagatgcgg
     4021 atggcattcg tgcgggcctc ggccggcgag agcgccgggc cggaaggcgt cgaagaggac
     4081 gaagcggcgc agggcggcga gaagacagag gcgggtgcgg aggctgcagg ggaaggggat
     4141 aacggtggcg acagctgaca tcggggtgtt cggagggtcg ggattctact ccttcctgga
     4201 cgacgtggag gaggtcccgg tcgagacgcc ctacgggccc ccgagcgaca gcttcttcat
     4261 cggtgaggtg ggggggaagc gggtggcctt tcttccgcgg catgggcgcc gacaccaact
     4321 gccgccgcac atgatcaacc accgtgccaa cgtgtggggg atgaaatcac tcggcgtcga
     4381 ccggatcatc ggtccttgtg cctccggcag tctgaagagg gaggtcgggc tggggcactt
     4441 cgtcgtctgc gaccagttcg tcgacaggac gacgggtcgc aaggacacgt tctacgacgg
     4501 gccggtgtcc acccacgtct ccgcggccga cccgtactgt cccaccatcc gcgaggcgct
     4561 cgtgcggaag ggccgggaac tcggcatcac catgcacgat gggggcaccg tcgtcaccat
     4621 ccaaggaccg cgcttctcga cccgggccga gagccggtgg tttgcgtcgg cgggttggga
     4681 ggttatcaac atgaccgcgt acccggaggg ctacctggcc cgtgagttgg agatctgcta
     4741 cgcgaacgtg tccctggtta cggactacga cgtaggcgta gccgacgatc cgtcagtggc
     4801 ggcggtgtcg cacgcggatg tggtggcggt gttcgagcag aacaacgcgc gactgcggga
     4861 gttgctgttc gcggtgatcc cggctcttgg cgggcgacgc gactgcgtgt gcgcttccgc
     4921 gctggcgtcg gcccggctgt agcaggcgag acccggggcg ccggccgccg ggtggcgcgg
     4981 gcccggccga cggatgtcga ccgagccgac cgagccgagc tcagtcggct ttcgcggggt
     5041 gatcgacgac gagcacgtcg tcttcgcgtc ggatgaccga actcttcacg tgggccacca
     5101 tggaagcctt gcgcgactcg tcggtctctc cggccatggc ggcgtcgagt tcgaggacca
     5161 tcccatacat ccgctgcacc aggtcggtcg attcttcggg ggtgaggccg agcggcttga
     5221 ccacgctcgc caccttcacg agaatggcct tgagcgccgt caacgtctcg cgttcgggct
     5281 cgtgcggcgc gccgttgcga tccggttggc tgtcgtagtc gtcgcgggat tctccccgcc
     5341 cgtctctgcg ttcgcgtcgt gccaccttca gaccctcttt cgtcgctctt tgtagaaatc
     5401 gaccgcgcgc ccgactccct cggcgaggtc caccttccac tcccaaccca acgtctctcg
     5461 cgccagggtg gcgtcgagcg cgatgtgttc cacctcaccg gggcgggtgg gggcgtactc
     5521 cggctcgata tccacaccca cagcatcgcg gacgctctcg aacacctgcc ggtctgagac
     5581 ctcgataccg cggccgatgt tgaacagccc gtccgatcca cgctcgatgg ccagcatgtt
     5641 ggcctcaacg atgtcgccta cgtagcagta gtcgcgcgtc ttggtgccgt ccccgaatat
     5701 gcggggcgtc tccccgttga gcagctgtag cgagaagatg gccaccaccc cggcctcacc
     5761 gtgcggatcc tggcgcgggc cgtagacgtt cggatagcgt agcacggtgt agtcaaggcc
     5821 gaagttggct cgatacgcgt agaggtagtg ctcgacggtg tgcttgctca cgccgtaggg
     5881 cgagatgggt tgcaccggat gcttctcgtc gacggggagg taagccgcct ctccgaacat
     5941 ggccccgccc gtgccggcgt agacaacctt gcgcgtgccg tggacgcggc aacactcgag
     6001 gaggttgagc gtgccgagaa ggttgacgtc ggcgtcgtag cgcgggtctg tcaccgactt
     6061 ccgcacgtcc atctgggccg cgtggtgtat gacgatgtcg gggcgttcgg cttcgaacac
     6121 ctccgcgagc cgcgcgtccc gcacgtctac ttcgtggaag cgggctccgg cggggatgtt
     6181 ctcccgcttg cccgtcgcca ggttgtcgac gatggccacg tcgaaaccac ggtccagcag
     6241 tctgtcggat acatgcgaag cgatgaagcc ggctccgccc gtgactagta ctcgcactgc
     6301 gcctcctcga tccccatccg cttgtccaac cccatcagtc ttcgattact atgcgcgtgt
     6361 cggtctcctt cacgcccgag acggtctgga tcgaacggaa gatcaattcg gccaaggctc
     6421 ggggatcttc cgcctccacc tgcacgatca gatccacggg gccggtgacg gcgtcggcac
     6481 gctggaccga gtcgatggtg cgcagcccat ccaggatctc cttcgccctc ccggcttcga
     6541 tcttcacgag tacatatgct ctgaccatga ccaccttcct cctgtgaggt tgcgcgatcg
     6601 caccgatgtc gctcctattc taatctaacc cgcggcccgg tgcgtgggcg gtgtcgtgag
     6661 gtggaaacag cctcgggata cgctatactc tggcctccga tgtgggtggt aggcgtcggg
     6721 acgtggcgca gcctggtagc gcatctgctt tgggagcaga gggtcggagg ttcaaatcct
     6781 ctcgtcccga cctctgaccg gctggggcga gcgatgcaac gggcgggtgt agctcagttg
     6841 gtagagcgtc ggccttccaa gccgtcggcc gcgggttcga atcccgtcgc ccgctcttag
     6901 aggccggtag gctcgttgtc cgaaggccgg ggaaaggcgc ctgtagctca ggtggataga
     6961 gcaacggact tctaatccgt gggccggggg ttcgaatcct cccaggcgcg ctggaggtgg
     7021 gagagtcgtc ggcgggactc cgtgcggcga tacgtggtga acgtagctca gtcggtagag
     7081 ctctggattg tggctccagt ggtcgcgggt tcaagtcccg tcgttcaccc ctcgcatatg
     7141 gtgaccctcc accgcactcg gctatggctg ggcaggtggg gggtcgtctg ttgccggcgg
     7201 tacgtcggcg agccgccgac gagcggcgcg gccgcccgac gccggctctt gccgtgcggg
     7261 tgtggcggaa tggcagacgc gcatgattta ggatcatgtg tctttgacgt ggaggttcga
     7321 gtcctctcac ccgcaccacg attacgaccc gggtcggccg cgcggagcgc gaccggcaca
     7381 tggaaggaag gaccgagttg aagaccaccg taggcgagaa agtcggcaac gacgtcaagc
     7441 tggagatcga ggtttcaccc gaagaggtgc aggacggcat agccgccacc gtgaagcaac
     7501 tctcgcgcga ggtgcgcatc ccgggtttcc gcaagggcaa agttcccacc gggatgatcg
     7561 tgcagcggtt cggcatggag gcgatctccc aacagatgct cgacgatcga ctctcgccgt
     7621 ggctcctgga ggccctcacc gaggccggcc tggatccggt ggatcgaccg gaggtggact
     7681 tcgacgaagc gcccgaggaa ggcaaaccgt tctcgttcac cgcggtcgtg acggtcatgc
     7741 ccaccgccga actcggagag tataagggcg tcgaagcgcc gcgcccggac gcaacggtcc
     7801 tggactccga ggtcgacgag cgtgtcgagc gtctgcggga ggagttcgcc gaactgaagg
     7861 ccgtgggcga ccgtcctgcc gccgagggcg acttcgtgac ggtgaacgcc gagggttacc
     7921 gcgacggcga agtcgtggcc aacactcagc tcgacgacta catgttcgag gtggggggcg
//
LOCUS       DASRMJ010000318         3947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_317129, whole genome shotgun sequence.
ACCESSION   DASRMJ010000318 DASRMJ010000000
VERSION     DASRMJ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317129"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /gene="nadB"
                     /locus_tag="VFD74_08765"
     CDS             <1..1678
                     /gene="nadB"
                     /locus_tag="VFD74_08765"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZK49672.1"
                     /translation="DGGDRPVTDSPRRPHLIARGDAKLRFRSYDVLVVGSGVGGLTAA
                     VGAARKWNVALLTKSTLSDTTTFLAQGGIAAAVSPLDSPDLHLGDTLKAGAGLCDERA
                     VRILVEEGPARVRELELLGTRFDHRDGELVLASEGAHSMPRVVRAGGDATGSVVASAL
                     MDVLASGGRVELHEGEFVVDLLIEDGACIGVLSLDADGALILNHSRAVVLAAGGAGQV
                     FSSTTNPDVATGDGHAMAYRAGAVLRDMEFMQFHPTALHMEENPTLLLTEALRGEGAH
                     LVDGRGERFMVGTHPQAELAPRDVVVRRMKEIMDRDGTDHVFLDARHLGCSFLRERFP
                     TVFGGLEKRGFDLCSALIPVSPASHYFIGGVVTDVWGKTSIPGLYSCGETSCTGIHGA
                     NRLASNSLLEGLVFGERIVRDLDRFLAQPSRPVRKFRLDLPEETWPGNPPEQIRRVRR
                     ALAETMTRLCGIVRDTDGLQEAARVIAELRDSLRPPAHDVEELEVFNLLTVARLMVEA
                     ASTREESRGVHLRSDFPCVDDDHWHRHISMEYDPDNDSTRVRVVGRAEEE"
     gene            1675..2526
                     /gene="nadC"
                     /locus_tag="VFD74_08770"
     CDS             1675..2526
                     /gene="nadC"
                     /locus_tag="VFD74_08770"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZK49673.1"
                     /translation="MNVDDTSSELIRLALREDLGGYGDVTSAWTVAPETRSEGFLVAR
                     DHAVISGLDLAAAVLAEVDREAIFERLAADGSTVEPGAKLARLEGRARSLLAAERTLL
                     NMLAHLCGVATQARRFSDAVAGTAAVVVDTRKTTPGLRVWEKKAVRDGGCANHRFGLF
                     DMVLIKDNHLVAGGGVGATVRRAREAAPFSMKVEVEVADEQGMREALASGADIIMFDN
                     VSGPELGRLVRLARDLAPAVLLEASGRVSLETVRAIAESGVDIISSSKLTAGAPPVDV
                     GLDFDLS"
     gene            2581..3348
                     /locus_tag="VFD74_08775"
     CDS             2581..3348
                     /locus_tag="VFD74_08775"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZK49674.1"
                     /translation="MLLAVDAGNTQTVLGLFEDSALGEEWRIATDVNRTGDELGALVD
                     TLLRARGLCRDTVTALALSSVVPALNREYVRMARRYFGVEPLVVGPGVKTGMPIMTSN
                     PRELGADLIVDAVAAFETVGGPCVVVDFGTATTFGAVSAKGEYLGHAIAPGIQTAMDA
                     LAARAAKLPRVELVDPGTVIGKTTVHSMQAGAVFGFAGQVDGLVTRMRAELGAPRAVS
                     VATGGLASLIFDHAATLDRLDEHLTLRGLEIIHRRNQ"
     gene            3356..>3947
                     /locus_tag="VFD74_08780"
     CDS             3356..>3947
                     /locus_tag="VFD74_08780"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HZK49675.1"
                     /translation="MGGGQAERPPAGSEPPAVDFRAPFSIGHVVIPNRVVLAPMAGLT
                     TSAFRRRAKAYGAGLVVTEMVSVYGLIYGDRRTHHYLEFEEEERPLALQLFGADPEAV
                     ARAVSIVIEAGHLPDLIDLNMGCPVRKVMKTGAGAALLADPERAQAVVAAAVRAAATA
                     GLPVTAKIRSGLEPGREVAVEVAKRLEAVGVAAVGVH"
BASE COUNT          600 a         1277 c         1435 g          635 t
ORIGIN      
        1 ggatggtgga gatcggccgg tgaccgactc accgagaagg ccgcatctca tcgcccgggg
       61 cgacgcgaag ctccgcttcc gtagttacga tgtcctggtc gtgggcagcg gcgtcggagg
      121 gctcacggcc gccgtcggcg cggcgcgcaa gtggaacgtg gcgctgctca ccaagagcac
      181 cctgtccgat accaccacct ttctcgccca gggcggcatc gccgccgcgg tcagtccgct
      241 cgactcaccc gatctccatc tgggcgatac cctgaaggcg ggtgcgggtc tgtgcgacga
      301 gcgcgcggtt cgcatactgg tagaggaggg gccggcccgt gtacgcgagc tggagcttct
      361 cggaacccgt ttcgaccacc gcgacgggga gttggtgctc gcgtccgaag gcgctcactc
      421 catgccgcgc gtggtgcgcg ccggcggcga cgccaccggg tcggtcgtgg cgagcgcgct
      481 gatggacgtg ctcgcttcgg gcggcagggt cgagttgcac gaaggcgagt tcgtggtcga
      541 ccttctcatc gaggacggcg cctgtatcgg cgtgttgtcc ctcgacgcag acggggcact
      601 catcttgaac cattcgcgcg ccgtcgtgct cgccgccggg ggtgcggggc aggtcttctc
      661 cagcaccacc aaccccgacg tcgcgaccgg agacgggcat gccatggcct atcgagcggg
      721 agcggtgctg cgcgacatgg agttcatgca gttccacccg accgcgctcc acatggaaga
      781 gaacccgaca ctcctgctca ccgaggcgct tcgcggcgag ggtgcccacc tcgtggacgg
      841 gcgcggcgag cgcttcatgg tggggacgca tccacaggcc gagctggccc cacgcgacgt
      901 cgtggtgcgg cgcatgaagg agatcatgga tcgggacggc accgaccacg tgttcctcga
      961 cgcccggcat ctcggctgct cgttcttgcg ggagcgcttc cccacggtct tcggcgggct
     1021 ggagaagcgc ggcttcgatc tctgctcggc gctcatcccc gtatctcccg cgtcgcacta
     1081 cttcatcggg ggcgtcgtca ccgacgtgtg gggcaagacc tccatcccgg ggctctactc
     1141 gtgtggggag acctcatgca cggggataca cggggcgaat cggctggcct cgaactcact
     1201 gctggaaggc ctcgtcttcg gggagcgcat cgtgcgcgat ctggaccgtt tcctggcgca
     1261 accgagtcgc ccggtgcgca agttccggct cgatctgccc gaggagacgt ggccgggcaa
     1321 ccctcccgag cagatcagac gagtccggcg cgcgctggcc gagaccatga cccgcctgtg
     1381 cggcatcgtg cgggataccg acggtctcca ggaagcggcc cgcgtcatcg ccgagctcag
     1441 ggactctctg aggccgccgg ctcacgatgt ggaagagctc gaggtgttca acctgctgac
     1501 ggtggctcgg ttgatggtcg aggcggcctc gacgagggag gagagtcgcg gagtgcacct
     1561 ccgctccgac ttcccgtgcg tggatgacga tcactggcac cggcacatat cgatggaata
     1621 cgatccggac aacgactcca cccgagtgcg tgtagtgggg agggcggaag aagaatgaac
     1681 gtggacgaca cgagcagcga actcatcagg ttggcgttgc gcgaagatct gggcggctac
     1741 ggcgacgtga cctccgcgtg gacggtcgcc ccggagacgc gctcggaggg gttcctcgtc
     1801 gcacgcgacc acgcggtgat ctctgggctc gatctggcgg cggcggtcct cgccgaggtg
     1861 gaccgggagg ccatcttcga gcggctcgca gccgatgggt cgacggtgga gccgggagcg
     1921 aagctggcgc gtctcgaagg cagggcccgt tcgctgctgg ctgcggagcg cacccttctg
     1981 aacatgttgg cgcatctctg cggcgtggca acacaagccc gccggttctc cgacgctgtg
     2041 gcaggcacgg cggctgtcgt ggtcgacaca cgcaagacca ccccgggcct gagggtgtgg
     2101 gagaagaagg ccgtgcgcga cggtggctgc gcgaatcacc gcttcggtct gttcgacatg
     2161 gtcttgatca aggacaacca tcttgtggcc ggcggcggcg tgggggccac ggtacgccgg
     2221 gcgcgcgagg cggctccctt ctccatgaag gtggaggtgg aagtcgccga cgagcagggc
     2281 atgcgggagg ccctcgcgtc gggggctgac atcatcatgt tcgacaacgt gtcaggaccg
     2341 gaactggggc gcctggtgcg cttggcgcgc gatctcgccc cggctgtcct gctcgaggcg
     2401 tccgggcggg tgagcctcga gaccgtgcgt gccatcgccg agagcggggt ggacatcatc
     2461 tcgagcagca agctcacagc cggcgcgccg ccggtggacg tggggctgga cttcgacctg
     2521 agctaggccg tccgcggcgg ccgacggccg ccgcgcgaca gtgacgagag aggagacgcc
     2581 gtgctgctgg ctgtcgacgc aggaaacaca cagaccgtct tgggcctctt cgaggactcc
     2641 gctctgggcg aggagtggcg catcgccacc gacgtcaatc gcaccggtga cgaactgggc
     2701 gcgctggtcg acacactcct gcgtgcccgc gggctctgcc gcgacacagt caccgcgctg
     2761 gctctctctt ccgtggtgcc ggcgttgaac cgcgagtacg tgcgcatggc tcggcgctat
     2821 ttcggagtgg agcccctggt ggtgggtccc ggcgtcaaga ccggtatgcc catcatgacc
     2881 agcaaccccc gtgaactcgg ggctgacctc atcgtcgacg ccgtcgccgc cttcgagact
     2941 gtgggcggcc cttgcgtagt ggtggatttc ggcacggcca cgacgttcgg agcggtgtcg
     3001 gccaaaggcg aatatctggg ccacgcgatc gccccgggta tccagacggc gatggacgcc
     3061 ttggcggctc gagccgcgaa actgcctcgg gtggaactgg tcgatcccgg gaccgtcatc
     3121 gggaagacca cggtccacag catgcaggcg ggagcggtct tcggcttcgc cggccaggtg
     3181 gacggcctgg tcacgcgcat gcgcgccgag ttgggtgcgc cgagggccgt gtcggtcgcc
     3241 accggggggc tcgcctcact gatattcgac cacgccgcca cgctcgatcg gctcgacgaa
     3301 cacctgactc tacgcgggct ggagatcatc caccgccgga accagtaggc gcgtcgtggg
     3361 tggagggcag gccgaacggc cgcccgcagg gtcggagccg ccggcggtcg acttccgcgc
     3421 gcccttctcg atcggccacg tcgtcatccc gaatcgagtg gtcctcgccc cgatggccgg
     3481 tttgaccacg agtgccttcc gccgccgcgc caaagcgtac ggcgcggggc tggtggtcac
     3541 ggagatggtg agcgtctacg ggttgatcta cggcgaccgg cgcacgcatc actacctcga
     3601 gttcgaggag gaagagcgcc cgctggcctt gcagctcttc ggcgcagacc ctgaagcggt
     3661 ggcgcgggcc gtgtcgatcg tgatcgaggc gggccatctg cccgatctca tcgacctcaa
     3721 catgggatgt ccggtgcgca aggtgatgaa gacgggcgcc ggggccgccc tgctggcgga
     3781 cccggagcgg gcacaggcgg tggtggcggc ggccgtgcgc gccgccgcca ccgcgggcct
     3841 cccagtcacg gccaagatca ggagcggtct cgagccggga cgcgaggtcg cggtggaggt
     3901 ggccaagcgg ctcgaggccg tcggagtagc tgcggtcggt gtgcatc
//
LOCUS       DASRMJ010000319         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_174456, whole genome shotgun sequence.
ACCESSION   DASRMJ010000319 DASRMJ010000000
VERSION     DASRMJ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174456"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1586
                     /locus_tag="VFD74_08785"
     CDS             <1..1586
                     /locus_tag="VFD74_08785"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZK49676.1"
                     /translation="TPTPAAGTPSAIVGAPGVANAAAVVASGRVVTVYRKIHLPNYAV
                     FDEERYFTPGDHVEVLEWGGERLSVNVCEDIWNPDGPTEAAALAGGARLVVNISMSPY
                     HIGKGGEREQMLRRRAVDAGAFVVYVNGVGGQDELVFDGHSVVAGPDGSLLARGAQFE
                     EELIIVDLDLAAAQASKTRVASVGRYRLETVRIDAAASAPPAAGHSASGPTAIPVAFL
                     APGAAAIHRPLLSRSAEIYAALCLGVRDYAGKNGFRGVVLGLSGGIDSALTACIAVDA
                     LGREAVTGVTMPSRYSSADTLSDARILAQNLGIDLLELPIEDIFVPYLETLAPAFADR
                     PSDATEENLQARIRGNLLMALSNKFGWLVLTTGNKSEAAVGYATLYGDMAGGFAVIKD
                     VPKTVVYELALHRNSLGAHAGPIPASTIERPPSAELRPGQTDQDTLPPYDLLDRIVEA
                     YVVRDESVASMVAAGLDRSEVERVVSLIDRNEYKRRQAAPGIRITLKAFGKDRRLPIT
                     NRYRDRFDPEEGLVSGGGA"
     gene            1671..>2166
                     /locus_tag="VFD74_08790"
     CDS             1671..>2166
                     /locus_tag="VFD74_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZK49677.1"
                     /translation="MTDPSSDAAPRFETQALHAGQVADPATGACAVPIYQTTSYAFED
                     TAQAAGLFELSQIGNIYTRIMNPTTSVFEARMAALEGGTAALAVSSGQAAITTAIINI
                     MGAGDHIVASSSLYGGTYNLFHYTLRKLGIEVDFVDASDPENFRAALKPNTRAFYGEM
                     IGNPR"
BASE COUNT          371 a          737 c          696 g          362 t
ORIGIN      
        1 ggacccccac ccccgccgcc gggactccca gcgccatcgt cggcgcgccc ggggtggcga
       61 acgcggccgc ggtcgtggcc agcggacgcg tcgtgaccgt gtatcggaag atccacctgc
      121 ccaactacgc cgtgttcgac gaggaacgct acttcacccc gggagaccac gtggaggtgc
      181 tggagtgggg tggtgagcgg ctgtcggtga acgtgtgcga agacatctgg aaccccgacg
      241 gccccaccga ggcggccgcc cttgcgggtg gcgcgcgcct cgtggtcaac atcagcatgt
      301 cgccctacca catcggcaag ggcggcgagc gggagcagat gctgaggcgc cgcgcggtgg
      361 acgccggtgc cttcgtggtc tacgtcaacg gcgtcggcgg ccaggatgaa ctggtgttcg
      421 acggccacag cgtggtcgcg ggccccgacg gatcgttgct ggctaggggg gcgcagttcg
      481 aggaggagtt gatcatcgta gacctcgacc tggcggcggc gcaggcatcg aagactcggg
      541 tggcgtctgt cggcagatac cgtctggaga ccgtgcgcat cgacgcggcg gcttccgccc
      601 cgcctgccgc cggccactcg gcctctgggc cgacggcgat ccccgtcgcc tttctcgccc
      661 ccggcgcggc cgcgatccat cgccccctgc tgtcccgaag cgcggagatc tatgcagcgc
      721 tgtgcttggg cgtgcgcgac tacgccggca agaacgggtt ccggggagtg gtgctgggcc
      781 tgagtggagg catcgattcc gcgctcaccg cgtgcatcgc cgtcgacgcc ctcgggcgcg
      841 aggcggtcac cggggtcacc atgccgtcac gttactccag cgccgacacg ctctccgacg
      901 cccgcatcct tgcccagaac ctgggcatcg acctcctcga actgccgatc gaggacattt
      961 tcgtgcccta cttggagaca ctcgctcctg ccttcgcgga ccgcccgtcg gacgccaccg
     1021 aggagaacct ccaggcacgc atccgaggca acctcctcat ggcgctctcc aacaagttcg
     1081 gttggctggt cctcacgaca ggcaacaaga gcgaggctgc tgtgggatac gccaccttgt
     1141 acggcgacat ggcgggcggt ttcgccgtga tcaaggacgt gcccaagacc gtagtctacg
     1201 aattggctct gcaccggaac tccctgggtg cgcacgccgg ccccatcccg gcatccacca
     1261 tcgagcgccc gccttcggcg gaactgcgtc ccggccagac cgaccaagac acccttccgc
     1321 cctacgatct tctggaccgc atcgtagagg cctacgtcgt gcgtgacgag agtgtcgcct
     1381 cgatggtcgc cgccggcttg gatcgctccg aggtcgagag ggtagtcagt ctcatcgacc
     1441 ggaacgaata caagcggcgt caggcggccc ccggcatccg catcaccctc aaggcgttcg
     1501 gaaaggaccg gcggctgccc atcacgaatc gctatcgtga tcgcttcgat cctgaagaag
     1561 gacttgtctc gggcggcggg gcgtagtaga ttctttgaat ccagattatc tttataagaa
     1621 ttcaattgtt ttccggcctg tgaggacgtc cggaagagag aggagccccg atgaccgatc
     1681 ccagtagcga cgccgcccca cgtttcgaga cacaggcgtt gcacgcgggc caggtggccg
     1741 atcccgccac gggggcctgc gcggtgccga tctaccagac gacctcatac gcgttcgagg
     1801 atacggctca agctgccggg ctgttcgagt tgtcgcagat cggcaacatc tacactcgca
     1861 tcatgaaccc aacgacgtcg gtcttcgagg cgcggatggc tgccctggag ggcggcacgg
     1921 cggctctggc ggtctcctcc ggccaagccg ccatcaccac agcgatcatc aacatcatgg
     1981 gagcaggcga ccacatcgtg gcctcgtcca gcctttacgg gggaacctac aacctcttcc
     2041 actacacgct tcggaagctg ggcatcgagg tcgacttcgt cgatgcctcc gaccccgaga
     2101 acttccgcgc ggcgttgaag cccaacacgc gggccttcta cggcgagatg atcggcaacc
     2161 cacgac
//
LOCUS       DASRMJ010000320         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_34240, whole genome shotgun sequence.
ACCESSION   DASRMJ010000320 DASRMJ010000000
VERSION     DASRMJ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34240"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..966
                     /locus_tag="VFD74_08795"
     CDS             100..966
                     /locus_tag="VFD74_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZK49678.1"
                     /translation="MSRLQGKVCLVTGSSGGLGRAFCRKLAAEGAVVAAAGRSLERLG
                     PLMAELDSMGAAGAAPGGAGGAGGAASGDAGGAAGTTDSAADAAVRTAAHLAVQLDTG
                     SWESVEAGVRTVEAALGRVDVLVNNAAAYGDLTRAPFWELPIEEWDRVMAANLRGPML
                     AARAVAPGMRARGCGRIINIVSATFWSGSANWAHYVASKGGLIGLTRVMAKELGPDGI
                     CVNAVAPGFTLTESSLDLMPGAETYGVDRGCLKRAQQPEDVVGLVAFLASDESGFITG
                     QTIIVDGGRQFN"
     gene            1082..2755
                     /locus_tag="VFD74_08800"
     CDS             1082..2755
                     /locus_tag="VFD74_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HZK49679.1"
                     /translation="MRTYDITPFEFDEAVDLQKYDAWFLDGTHSVPPWTPMFGWSWIN
                     YCRHGMMWAAQKLQLPTCKGWDWRLHRGGGYLAVLVIDDPVEIAAREKVFRKQLEPFI
                     EDYDAVWQKHLDELQGYYADLRAFDIAGATNIEMLDHLEDVLRLNRRMWEIHFYFMYV
                     VFGVYMLFEATMKQKFGIDDSDPTFHRVLTGFDNKVFQVDKKLWSFGKKAKAMGLDSI
                     FLENNPDKVGALLEQSEAGRLWLADFKEFLNEDGWRSQRMSEFINPTWIEDPTPAFMN
                     VRQFLQKGGDFDLDRERARLTVEREKAEKELLEKIPDEQKGWFTKLLRLAQRSGSFSE
                     EHNHWLDLYTHAMVRRALVGWGERLVAADVMDKVEDVFFLIPDEIRRVALTPEWHDLR
                     ALVADRRAEWVSWSETENPPMMGTVTLEEAMGTLIRSNDPVVLKVVVGSLPVPKPELK
                     ADLYGTCGSPGVVEGRARMVMSDADLLNIEEGDILVAPATYPSWTAVFALLGGVVVDR
                     GASLSHAAIVGREYGIPVVMNVFEGTKVIKDGMRIRASGNMGTVTILHD"
     gene            2873..>3757
                     /locus_tag="VFD74_08805"
     CDS             2873..>3757
                     /locus_tag="VFD74_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZK49680.1"
                     /translation="MTDKWIYWFEELTKASRELVGNKCANLGEMTRSGLPVPPGFALT
                     VDAYTRFMEETGAAGEVRDYVSSINQQQLVRDERHSRHIREVVERHDLPDDMKLDVSR
                     EYGRLCDLVDIADLPVAVRSSGVVSMPGAMESFLNIRGDEQLFKKVVEVWGSSFTHQA
                     VAYRATNDLSIEHFPIGVAVMKVVNAKCSGVAMSVHPNTGDRNKMLIEANWGLGEGVV
                     GGAITPDHFVIDRTTGDIHATISTKAQCVLPNGDGTSWQPVDPELQDCSCVSDEELRE
                     IVTLAKRLEEAYGEPQDIE"
BASE COUNT          687 a         1210 c         1278 g          582 t
ORIGIN      
        1 cttctgggaa cagtcaggcc gcacgtacta ggaagcgacg atcgggggcg cgacgcgggc
       61 gatcggggga tcgtctcggc cgcgaggggg gacagcatca tgagcaggct gcaggggaag
      121 gtctgtctag tcaccggttc gtccggcggc ttggggcgcg ccttctgccg caagctcgcc
      181 gccgagggag ccgtggtcgc ggccgccggc cgcagcctgg aacgcctggg acccctgatg
      241 gcagagctcg actccatggg cgcggccggg gctgcacctg ggggcgcagg cggcgcgggc
      301 ggcgcagcct ccggggacgc gggtggtgcc gccggcacca ccgactctgc cgccgacgct
      361 gccgtccgca ctgccgccca cctggctgtg cagctcgaca ccggttcttg ggagagcgtg
      421 gaggcgggcg tccgcaccgt cgaggctgcg ttggggcgcg tggatgtgct tgtcaacaac
      481 gctgccgcct acggcgacct gacccgcgcg cccttctggg aactgcccat cgaagagtgg
      541 gaccgcgtca tggccgccaa cctgcgcggg cccatgctgg cggcccgcgc cgtggccccg
      601 ggcatgcggg ccaggggatg cggccgcatc atcaacatcg tctcggccac cttctggagc
      661 ggatcggcga actgggcgca ctacgttgcc agcaagggcg gcctcatcgg gttgacccgt
      721 gtcatggcca aggagctcgg tcccgacggc atctgcgtga acgccgtcgc ccccggcttc
      781 accctgaccg agtccagcct cgatctcatg ccgggcgcgg agacctacgg cgtggaccgg
      841 gggtgcctca agcgggcgca gcagccggag gacgtcgtgg gcctggtggc cttcctcgcc
      901 tccgacgaga gcggcttcat caccggccaa acgatcatcg tcgacggcgg ccgacagttc
      961 aactagacgc gtggggtcca cgcggccgag gggccacggc gaacgtggcg aggaggtgac
     1021 ggacaggaag caggacagcc gtgcgcgcgt gtgaaacgag acacttctaa ggggggattg
     1081 aatgaggacc tacgacatca cgcccttcga gttcgacgag gcagtcgatc tccaaaagta
     1141 cgacgcctgg ttcctcgacg gcacgcactc cgtgcctccg tggaccccga tgttcggatg
     1201 gagttggatc aactattgcc gccacggcat gatgtgggcg gcccagaagc tccagctgcc
     1261 aacgtgcaag ggttgggact ggcgcctgca ccgcggcggc ggctacctgg ccgtgctggt
     1321 gatcgacgac cccgtcgaga tcgccgcccg cgagaaggtg ttccgcaagc agttggagcc
     1381 cttcatcgag gactacgacg ccgtctggca gaagcacctc gacgaacttc agggctacta
     1441 tgccgatcta cgcgcctttg acatcgcggg cgcgaccaat atcgagatgc tcgaccacct
     1501 ggaggacgtc ctccggctca accgtcgcat gtgggagatc cacttctact tcatgtacgt
     1561 ggtcttcggc gtgtacatgt tgttcgaagc cacgatgaag cagaagttcg gcatcgacga
     1621 ctccgacccc acgttccacc gggttctcac cggcttcgac aacaaggtgt tccaggtaga
     1681 caagaagctg tggagcttcg gcaagaaagc caaagccatg ggtctcgaca gcatcttcct
     1741 cgagaacaac cccgacaagg tgggcgctct gctcgagcag agcgaagccg gtcgcctctg
     1801 gctggccgac ttcaaggaat tcctgaacga ggacggttgg cgcagccaac gcatgagcga
     1861 gttcatcaac cccacgtgga tcgaggaccc gacccccgcc ttcatgaacg tgcgccagtt
     1921 cctgcagaag gggggcgact tcgacctgga ccgtgaacgc gcccgcctca ccgtggaacg
     1981 ggagaaggcc gagaaggagc tgctcgagaa gatccccgac gagcagaagg gctggttcac
     2041 gaagctgctg cgcctggcgc aacgctcggg ttcgttcagc gaggagcaca accactggct
     2101 cgatctctac acccacgcga tggtgcgtcg cgccctggtg ggctggggcg agaggttggt
     2161 cgcagccgac gtcatggaca aggtggagga cgtgttcttc ctcatccccg acgagatacg
     2221 ccgcgtggcg ttgactcccg agtggcacga cctgcgtgcc ctcgtcgccg acaggcgcgc
     2281 cgagtgggtg tcctggtccg agacggagaa cccgcccatg atgggcaccg tcacacttga
     2341 agaagccatg ggcaccctca tccgctccaa cgacccggtg gtgctaaagg tggtcgtcgg
     2401 gtcgctgccg gtgccgaagc cggaactcaa ggccgacctc tacggcacct gcggatcccc
     2461 gggcgtggtc gaaggcaggg cccgcatggt catgtccgac gccgacctgc tgaacatcga
     2521 agagggcgac atcctggtgg cgcccgccac gtacccctcg tggacggcgg tcttcgccct
     2581 cctgggcggc gtggtcgtgg accgtggggc cagtctctcg cacgccgcca tcgtcggccg
     2641 tgagtacggg atccccgtgg tgatgaacgt cttcgaaggc accaaggtca tcaaggacgg
     2701 gatgcgcatc cgcgcgtcgg gcaacatggg caccgtcacc attctgcacg actgagacga
     2761 cgagtcgtgg gacgaggtgg cggcaccggg catccgcccg gtgccgccac ccgcgcccgg
     2821 agcgcgcgcc gcgctcgggc ccacatgagt aggaacgagt aggagagcga ctatgaccga
     2881 caagtggatc tactggttcg aggagctcac caaggcctcg cgcgaactgg tgggcaacaa
     2941 gtgcgccaac ttgggtgaga tgacgcgctc gggtctcccc gtgcctccgg gattcgccct
     3001 gacggtggac gcctacacgc gtttcatgga agagaccggc gccgccggcg aggtgcgcga
     3061 ctacgtctcg agcatcaacc agcaacaact ggtgcgagac gagcggcaca gccgccacat
     3121 cagggaggtc gtcgagcgcc acgaccttcc cgacgacatg aagctcgacg tgtcgcgcga
     3181 gtacgggcgg ctgtgcgacc tcgtcgacat cgccgatctt ccggtagccg tgcgctcgtc
     3241 gggcgtcgtg agcatgccgg gagcgatgga gagcttcctc aacatccgag gcgacgagca
     3301 gctgttcaag aaggtcgtgg aagtgtgggg cagctcgttc acacaccagg ctgtagcata
     3361 tcgggcgacc aacgacctct ccatcgagca cttcccgatc ggcgtggcgg tgatgaaggt
     3421 cgtgaacgcc aagtgctcgg gcgtggccat gtccgtgcac ccgaacaccg gcgaccgcaa
     3481 caagatgctg atcgaagcga actggggcct cggagaagga gtggtgggtg gagccatcac
     3541 ccccgaccac ttcgtcatcg accgcaccac gggcgacatc cacgcgacca tcagcaccaa
     3601 agcccagtgc gtcctgccga acggtgacgg caccagctgg cagccggtcg atcccgagct
     3661 acaagactgc tcgtgcgtct cggacgagga gttgcgcgag atcgtcacgc tggccaagcg
     3721 gctcgaagaa gcgtacggcg agccgcagga tatcgag
//
LOCUS       DASRMJ010000321         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_430721, whole genome shotgun sequence.
ACCESSION   DASRMJ010000321 DASRMJ010000000
VERSION     DASRMJ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430721"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFD74_08810"
     CDS             <1..262
                     /locus_tag="VFD74_08810"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HZK49681.1"
                     /translation="DVTILEWVSANGAVLLSCDLDFGAILAAAGASGPSVIQIRGRDV
                     LPEVIADRVATVLSDLERELQTGALVSLDTDQARVRVLPLRS"
     regulatory      480..583
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            679..1362
                     /locus_tag="VFD74_08815"
     CDS             679..1362
                     /locus_tag="VFD74_08815"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HZK49682.1"
                     /translation="MHAVIVAGSTLDGPLDPEALARADLLIAADGGADAIEAAGLLPH
                     LLVGDLDSIGTDTHHAFAEAGVETVVFPAAKDETDSEIALRAAVQRGADTITVYGALG
                     GPRLDHLLGVVLLLTADWLQGVDVRLVDQRQKIYLAGGDRVVAGAVGDLVSLLPLTPV
                     VEDIHTEGLLYPLCGEALRQGAARGVSNELVVPRAWITHGFGRLLVTHFRGEHAAVTD
                     ENAQRKELP"
     gene            1509..2705
                     /locus_tag="VFD74_08820"
     CDS             1509..2705
                     /locus_tag="VFD74_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003957197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HZK49683.1"
                     /translation="MAASAPRADGAAAAAAALVVFGVFLMALLVGLAGCGGGGVGSST
                     TGTATGGTSTDGTATGGTSSGDTAAGGGEEPTEIVLMTHDSFAVSDDVLAAFTRETGI
                     RVRVLTSGDAGIMLNQAILTRDNPLADVLYGVDNTFLSRALDAGLFEPYAPPALDRVP
                     DALELDPEHRVTPIDFGDICLNYDKEYFASSGLAVPTSLADLVKPEYRGLLVVENPAT
                     SSPGLAFLIATVAEFGEGGEYTWQDFWVGLAANDVLVASGWEEAYYSDFSGGAGEGDR
                     PLVVSYASSPPAEVIFSEQPLTEAPTGVIVDGAFRQIEFAGILAGTKAPRGARLFLDF
                     MLSLEFQEDMPLNMFVFPANSDAALPPEFLEFTHVPPNPVSVDPWAIESDRERWIEEW
                     TAIVLS"
BASE COUNT          420 a          922 c          943 g          471 t
ORIGIN      
        1 agatgtgacc atcctggaat gggtgagcgc caatggggcc gtcctgctct cctgcgacct
       61 cgatttcgga gcgattctgg cggcggcggg cgcttcgggt ccaagcgtca tccagatccg
      121 cggcagggac gtgctgccag aagtgatcgc cgacagggtc gcaacggtcc tctccgacct
      181 cgagcgtgaa ctccaaacag gggcactggt gtcgctcgac acggaccagg cgagagtgcg
      241 tgtgttgccc ttgagatcgt agcgggttgc gaagtcagcg aggtcgtgaa catggcccgc
      301 ggcatggccg ccgtcctccc gctggagcag gccctcgatg ctcactcgca tgcgatctcg
      361 gcgctgcttc gagagaccgc caggccggcg gcgctggcgg cctctgcacg agcggcgtat
      421 actgcggggt ggtcggcgca aggctattcc cgtcctgtgg gcgggaccac gcggcgcgcg
      481 gaccgtgcta ggggagcgct cggcgctgag agtgtggctc gaccactgac cctgcgaacc
      541 tgatctgggt aatgccagcg aagggaagca tcggttgtct cgtaaggccg gggcgcggca
      601 cctatggtgc gcgccttttc tcattgtcgg aggccctccc ggcacgccca tgggtcgagg
      661 agcggaggag tcttcgtcat gcatgcggtc atcgtagcgg gatcgaccct cgatggacct
      721 ttggacccgg aagcgctcgc ccgcgccgac ctgctcatag cggccgatgg cggcgccgac
      781 gccatcgagg cggccggact gttgccccat ctgttggtcg gggatctcga ctccatcgga
      841 accgatacgc accacgcatt cgcggaagcc ggcgtggaga cggtcgtctt ccccgcagcc
      901 aaggacgaga ccgactccga gatcgcgctg cgcgcggctg tgcagcgggg agcagacacg
      961 atcaccgtgt acggtgcgct cggtggcccc cgcctcgatc accttctggg agtggtgctg
     1021 cttctgaccg ccgattggct gcagggcgtc gacgtccgtc tggtcgacca gcgtcagaag
     1081 atctacctcg cggggggcga ccgggtcgtc gcgggcgcag tgggcgatct cgtgtctctt
     1141 ctgcccctga ctccggtggt ggaggacatc cacaccgagg gactgctcta cccgctgtgc
     1201 ggtgaagcgc tccggcaggg agccgctcgg ggagtgagca acgaactcgt ggtgccccgc
     1261 gcttggatca cgcacgggtt cggccgtctc ctggtgacgc acttccgggg cgagcacgca
     1321 gcggtcactg acgagaatgc acaacgaaag gaactgccgt gatacttctg acttcatccg
     1381 ccggcaccac gccgtcggcg cggacgccgc tgtcgtcgca ggcgcaactt cggacgccgt
     1441 cgccgtcgca ggcgccattg ccgccgccac cgcagacgtc gtcaccacgg ccgagacggg
     1501 cgaggcgggt ggcagcctcc gcaccacggg ccgacggcgc agccgcggcc gcagccgcgc
     1561 tggtggtctt cggcgtgttc ttgatggctc tcctcgtggg gcttgccggc tgtggggggg
     1621 gaggcgtcgg ttcctccact acgggcaccg cgactggtgg cacctcgacg gacggcaccg
     1681 cgaccggcgg cacctcgtcg ggagacaccg cggccggcgg tggcgaagag cccacggaga
     1741 tcgtgctcat gacccacgac tcgttcgccg tgtcggacga cgtgctggcc gccttcaccc
     1801 gggagacggg catacgggtg cgggtgctca cctccggcga cgcggggatc atgctgaatc
     1861 aggccatcct taccagagac aaccccctgg ccgacgtgct ctacggggtg gacaacacct
     1921 tcctctcgcg agctctggac gcggggttgt tcgaaccgta cgccccgccc gcgctcgatc
     1981 gcgtgcccga tgctctcgaa ctcgacccgg aacaccgggt cacacccatc gacttcggcg
     2041 acatctgttt gaactacgac aaggagtact tcgcctcgag cggactcgcc gtcccgacgt
     2101 ctctggccga cctggtcaag cccgagtacc gcggtctgct tgtggtggag aatccggcca
     2161 cctcctcgcc cggtctcgcc ttcctcatcg ccacggtggc cgagttcggg gaggggggcg
     2221 aatacacgtg gcaggacttc tgggtcggcc tggctgcaaa cgacgtgctg gtcgcctccg
     2281 ggtgggaaga agcgtactac tccgacttct ccggaggagc aggcgagggg gaccgaccgc
     2341 tggtggtgtc gtacgcctcg tcgccacccg ctgaagtcat cttctcggag cagccgctca
     2401 ccgaagcccc caccggcgtg atcgtcgacg gcgccttccg tcagatcgag ttcgccggga
     2461 tcctggcagg cacgaaggct ccccgcggcg cgcgtctctt cctcgacttc atgttgagcc
     2521 ttgagttcca agaggacatg cctctcaaca tgttcgtgtt cccggccaac agtgacgcgg
     2581 ccttgcctcc ggagttcctc gagttcaccc acgtgccccc gaaccccgtc tccgtggacc
     2641 cctgggccat cgagtccgac cgcgaacgct ggatcgaaga gtggaccgcg atcgtgttgt
     2701 cgtaaacagg tggtggcggc ggccgggatg ggccgccgcc accgaaggcg ggcgcc
//
LOCUS       DASRMJ010000322        13002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_34307, whole genome shotgun sequence.
ACCESSION   DASRMJ010000322 DASRMJ010000000
VERSION     DASRMJ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 13002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13002
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34307"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1594
                     /locus_tag="VFD74_08825"
     CDS             176..1594
                     /locus_tag="VFD74_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZK49684.1"
                     /translation="MSKPFSVFWGCTIPARFPFIEKSTRLVLEATGGRPVDVEGYTCC
                     PEGTLVKAVDEEAYYVTAARNLSLASAAGLPMVTPCNGCYSTLKSVSSTLRADWRLAG
                     RISALIADGATHAADAADFAQSADIADAVHAAGHAPHPPDAPIFPEVKHLAEWLFDEL
                     GPAAISKRVVKPLWGMRLAVHYGCHLLRPSPAVRWDSATHPTKLEDLVRALGATVVDY
                     DTKMACCGGALDRVGERDGALEFCRAKLDDLAGVGVDGLVVVCPSCLQQFDLNQASLL
                     RGGERTPIPVFYYSELFALTLGFQPEELGLSMHRVSVEPFLAAWAEREDRRRVIAEEF
                     SVPELQKCADCKACEDDCPVTKVAGGFSPTGIIGEILAGDLEGVIERGDLWKCLECFT
                     CYERCHSRLGMAEVFRKLKELANEREKVPAAVRSAYEMFRATGSLGEPRESARARLGL
                     APLPARGGDDLERILSRREERR"
     gene            1822..2910
                     /locus_tag="VFD74_08830"
     CDS             1822..2910
                     /locus_tag="VFD74_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HZK49685.1"
                     /translation="MGVCDESGSRMSGELAVRSMASMHDRGNGLGGGFAGYGIYPDFP
                     HAFCFHMLYNDDPSKDDTEYYLARHFVILHHEPIPSKSVRGIQDEPILWRYFLEITDQ
                     RLAETQLTAEDYVVHAVMDINDQIRGAYVASSGRNMGAFKGVGFPEEIGEFYRLDEYK
                     GWTWTGHGRFPTNTPGWWGGAHPFCLLDYSIVHNGEISSYGINRRYLEMFGYNCSMRT
                     DTEVVTYLFDLMLRRHGLPVEIACKALASPFWKDIDLMGEEDKRLYTALRSVYGSALL
                     NGPFAVVLGRGEGMVALSDRIKLRPLVAARHGSVVYVASEESAIREICPRPERSWMPR
                     AGEPVIAQLHDGVNPVTSLAAASAGVTA"
     gene            2930..4447
                     /locus_tag="VFD74_08835"
     CDS             2930..4447
                     /locus_tag="VFD74_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZK49686.1"
                     /translation="MTFVRPEFRVRIDREECTKCGRCVHQCGWGVYHLDDDGRPIPDH
                     AKCAACHRCVTFCPADAITIEKNPLAYKESDSMSAAHRKAIWRQAETGGILLTGMGND
                     KPYLRIFDHLLLDACQVTNPSIDPLREPMELRTFLGRKPDFVEVEWGSDVGAKPRLLT
                     ELDPQLTLETPIMFGGMSFGSVSLNVHRALAMAAKASGTFMNTGEGGLHADLEPYQDH
                     IIVQCASGRFGVHTDYLQAGAAVEIKIGQGAKPGIGGHLPGEKIDEQVSTTRMIPQGT
                     DALSPAPHHDIYSIEDLRQLIYAIKEATGYKPVSVKIACVHNVAAIASGVVRAGADIV
                     YLDGFRGGTGAAPTVIRDHVGIPIEIAIAVVDQRLRDEGIRNRASIVAAGGIRSSADV
                     AKAIALGADACGIGTPALVALGCHVCQKCHTGCCSWGICTQRPELTRRLDPEWGAERV
                     SNLVRAWSHEIAEILGALGVNAIESMRGSRERLRALGLDQSTMDMLGVKPAGMGH"
     gene            4599..5336
                     /locus_tag="VFD74_08840"
     CDS             4599..5336
                     /locus_tag="VFD74_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49687.1"
                     /translation="MAHIDARGIYYKTLNEQIREQVAVGVTTVVLDHVNGQRYIGAGI
                     SADGLTIVVNGVPGNDLATFMDGPTVVVTDNAQDGIGNTMNRGLVAVEGDAGDVIGYG
                     MRGGRIYVRGDVGYRVGIHMKAYERHIPVIVIGGKARDFFGEYMAGGEMVLLGLGETD
                     EPVVGSYVGTGMHGGVIFVRGEVAPHQLGKEVGLACPTAADEARIEELVRDFCRVHGL
                     DAAPVLAAPFVKVAPFTHRPYGKLYAY"
     gene            5497..6816
                     /locus_tag="VFD74_08845"
     CDS             5497..6816
                     /locus_tag="VFD74_08845"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZK49688.1"
                     /translation="MTKDDVLRIVREQDIKFVRFWFTDVLGFLKSFAVTPSELEGAFS
                     EGMGFDGSSIEGFTRIQESDMVAMPDPGTFNVLPWRRGSGGTARMFCDIQMPDGKPFP
                     GDPRYILKRLLKKAADLGYTMYLGPELEFFYFKDSKGTEVLDRGGYFDLTPLDVATDL
                     RRDTILALQDLGIDVEYSHHEVAPSQHEIDLKYKDALTMADQVMTYRLTVKEVAQSRG
                     TYATFMPKPMYGENGSGMHTHQSLFTGPRNAFFDGNDEYHLSSAGKSYIAGLLKHARE
                     MCLVTSQWVNSYKRLVPGYEAPIYCCWARRNRSAMVRVPMYKPGKEEATRVEMRNPDP
                     ACNPYLAFAVMLAAGLKGIEEGYELAPESTNNIYEMTDGEREAAGIDSLPVDLYEAIN
                     EFKGSALMREALGDHVFEYLIRNKQAEWDEYKAIVTPYELDRYLAVL"
     gene            6961..9681
                     /gene="polA"
                     /locus_tag="VFD74_08850"
     CDS             6961..9681
                     /gene="polA"
                     /locus_tag="VFD74_08850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZK49689.1"
                     /translation="MNDHGNDAPEHGPLFLIDGNNLAYRAFFALPETISTSAGFPTNA
                     LYGFCSMMIKVLAEFAPEVVIVAWDSREKTFRHEDFEEYKAHRKPMPDLLREQWPHLR
                     ELSEAFGFVNLNVPGYEGDDILGTLARQGDAEGRQVVIVTGDRDALQLVTANVTVMSN
                     TRGITDVKLYDPHAVEERFGVPPSLIPDLIGLKGDSSDNIPGVPGIGEKTAALLLQEY
                     GSLEEVLAHAGEVKAPKRRELLLKHADDARMSKELARLDREVPIDIHAAEVAPHHLDR
                     ARLAELLHRFEFSSLLDRLGSEPLARVVDIPAPARAHGPGLRIEQVGLRELARELAVP
                     GRIGLAGARQDGLSRLWLARRGAADGTVPAGPSVDPAPPADAGATPLADATPPADATA
                     FALDFSETESDALRALLARSEPACHDFKSDPLLPGLLRSVSHDTMVAAYLLAPGRRSY
                     ELSDLAVQAGVSWLDVEETSGLDGPDGHATRALAALDVSRFQERALRETGMWDLFRDV
                     ELPLSRALLDMEAVGIHLDCYRLGEITAKVEDQLDGLEAQAYEAAGEEFNLGSPQQLA
                     GVLFEKLGLPRQRKTKTGYSTDAHTLDALRDLHPVIELIETHRELTKLLSTYLLALPS
                     AVDPATGRLHTTFHQAVAATGRLSSSDPNLQNIPVRTDLGARIRGCFTAEEGRVLVVA
                     DYSQIELRIMAHLSGEPTLLDAFRRGEDIHTRTAAEVFDLPEEEVDATHRRYAKAVNF
                     GIMYGISAFGLARQLKIPQNVAGEYIERYSARMPHVRAFIEATISDASAQGFVATIMG
                     RRRPIPELRSGNQQTRQLGERLAVNSVIQGSAADIIKVAMLRCRDRLARESRETRLVL
                     QVHDELIFECPLAAGRSVARMVREEMVGSWVVDPPLIVDVGVGEDWLSAK"
     gene            9837..11519
                     /gene="rpsA"
                     /locus_tag="VFD74_08855"
     CDS             9837..11519
                     /gene="rpsA"
                     /locus_tag="VFD74_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZK49690.1"
                     /translation="MPDQLTDENARTLQGGQDLMIEVDGEMIPNYDATMVTFEEGDIV
                     TGTVVRVDKDEVLVDIGYKSEGVVPVNELSIKKSVDPSDEVTVGDVIDALVLTKEDAD
                     GRLILSKKRARFERAWQRIEKAAEEGTPVEGTVIEVVKGGLILDLGVRGFLPASLVDI
                     RRVADLDEFVGQPLLCRVIELNRFRNNVVLSRRAVLEEERRGVREQILDRMNMGDFVT
                     GTISNIVDFGAFVDLNGIDGLIHISELSWSHVNHPSEVLTINQEVTVKVLDIDRDRQR
                     ISLGLKQTQKDPWQEVFENRQVNEIVHGRVTKLVSFGAFVEIEEGVEGLIHISELAQH
                     HVEDPSEVLRNGMEVNVKIIEIDPDRRRLSLSLKRLEPEYRIHEDAMAQEIEAEEAVA
                     VEAAEAGAEVDEGAGIESTAVAEGDASTEADGGAEAEPEAHAEAEAEAAAEADAEAEP
                     EADAEAETAAEAEVEAEAGEEAAPESEAVVESEAQPEAVIDTPDEAASEDATEAGADT
                     ASDAGAETLTGSIEDAGEDVGEDAPVEEAVVDESDALDADSLKLSDEVFEAE"
     gene            11643..12335
                     /gene="coaE"
                     /locus_tag="VFD74_08860"
     CDS             11643..12335
                     /gene="coaE"
                     /locus_tag="VFD74_08860"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.4"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZK49691.1"
                     /translation="MKVCPTPALESRADPMSERRLQADGRAAIGLTGGLASGKSSALE
                     MFARLGARVISADAIVHGLYELAEVRSALRARFGDGIFDTSGAVDRRALSSRLSRRGD
                     DLAAVESIVHPHVEGELRAFVAHGPRGSVNVCEVPLLFEGGLQGLFDLVLTIEAPAAV
                     RRERASSRMTPDVFEWLDARQAGEEARSAGADLAYANTGSPEELRTFVTGVYERAQAM
                     ATGRAEGSHARA"
     gene            12406..12975
                     /locus_tag="VFD74_08865"
     CDS             12406..12975
                     /locus_tag="VFD74_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZK49692.1"
                     /translation="MFLIVLGLALLVVGLVASGVGGELKTRVESRLYPLKFEEHILRA
                     ATAYGVDPYLVAAVAKAESGFEPGAVSPVGAVGLMQIMPETAVWIQQRPDWKGGAVPD
                     LTDPAANLEMGAYYLAYLTQLFAGDLRSTLAAYNAGQGTVTGWLTLSGSGTGLQSGDI
                     LFAETREFVARVEKLRARFLRAHPGAFVT"
BASE COUNT         2280 a         4098 c         4415 g         2209 t
ORIGIN      
        1 ccctgcggcg acccccctaa ctcgaggtac gacaggatac caggcccgcc gatcccctat
       61 tttgaatagg ggatcggcgg gcctggtatc ctgtcgtacc tcgagttagg ggggtcgccg
      121 cagggtgggg cggtcggctc ggggtctgca cacacgtcgg cacgacaggt gggatatgtc
      181 caagcccttc tccgtcttct gggggtgcac catccccgcc aggttcccgt tcatcgagaa
      241 gtccactcga ctcgtgctcg aggcgaccgg cggtcgtcct gtcgatgtgg agggctacac
      301 gtgctgtccc gaggggacgc ttgtgaaggc ggtcgacgaa gaggcctact acgtgacggc
      361 cgcgcgtaat ctctcgttgg cgagcgccgc cgggctgccg atggtgacgc cgtgcaacgg
      421 ctgttactcg acgctgaagt cggtctcgtc gacgctgcgg gcggattggc ggctcgccgg
      481 ccggatctcc gcgctgatcg cggacggcgc cacgcacgcc gccgacgccg ccgacttcgc
      541 ccaatctgcc gacattgccg acgccgtcca cgccgccggt cacgcccccc acccgccgga
      601 cgcgccgatt tttcccgagg tgaagcatct cgctgagtgg ctcttcgacg aactgggccc
      661 ggccgccatc tcgaaacgcg tggtcaagcc gctctggggc atgcgcctcg cggtgcacta
      721 cgggtgccat ctgctgcggc ccagtcctgc cgtgcgctgg gacagcgcta cccatcccac
      781 caagctcgag gacttggtgc gcgctctggg cgcgacggtg gtggactacg acaccaagat
      841 ggcgtgttgc ggtggggctc tcgaccgggt gggagagcgt gacggggccc tcgagttctg
      901 ccgcgccaag ctcgacgatc tggccggcgt gggcgtcgac ggcctggtgg tggtgtgccc
      961 ctcctgtctc cagcagttcg acttgaacca ggcgtctctg ctccggggcg gcgagcggac
     1021 gcccataccc gtcttctact actcagagtt gttcgctctg acgctcggct tccagcccga
     1081 ggagttgggc ctgtccatgc accgggtgag cgtcgagccg ttcttggccg cgtgggcgga
     1141 gcgtgaagac cgacgccggg tgatcgccga ggagttctcc gtgccggagt tgcagaagtg
     1201 cgccgactgc aaggcgtgcg aggacgattg cccggtgacc aaggtggccg gaggcttcag
     1261 ccccacgggc atcatcgggg agatactcgc cggcgacctc gaaggcgtga tcgagcgggg
     1321 cgacttgtgg aagtgcctcg agtgcttcac ctgctacgag cgctgccata gtcgattggg
     1381 catggccgag gtctttcgca agctcaagga gttggcgaac gagcgggaga aggtgcccgc
     1441 cgccgtgcgc tccgcatacg agatgttccg ggccactgga tctctggggg agccacggga
     1501 gagcgcgagg gccaggctgg gcttggctcc gttgcccgca cgcggtgggg atgatctcga
     1561 gcggatcctg tccaggcgag aggagcgtcg atgagcgccg cgaccggcga tccgggccgt
     1621 cgtgcgggtg gggaccggcc cggccgccgc ggcctgcccg acgtcgcccg tggcctgaat
     1681 cgcttcggcg agtcggatcg gcgcagtgcg gtgcgaccat acggcgggct gccgtccccc
     1741 gggtcgcgct cgagcgatct tgcgccgcgt ccggcctaca cggccgaggc cgatgagcgc
     1801 atcctgggcg cgtgcggact catgggtgtg tgcgatgagt cgggcagccg gatgagcggc
     1861 gagttggcgg tccgcagcat ggcctccatg cacgaccgtg ggaacgggct gggcggtggc
     1921 ttcgccggat acggcatcta cccggacttt ccgcacgcct tctgcttcca catgctctac
     1981 aacgacgatc cctccaagga cgacaccgag tactacctcg ctcggcactt cgtgatcctc
     2041 caccacgaac ccatcccgtc gaagtcggtg cggggcatcc aagacgaacc catcctctgg
     2101 cgctacttcc tcgagatcac cgaccagcgc ttggccgaga cgcagttgac cgccgaggac
     2161 tacgttgtgc atgcggtcat ggacatcaac gaccagatca ggggggccta tgtggcctcc
     2221 tcgggcagga acatgggcgc gttcaagggg gtgggttttc ccgaagagat cggagagttc
     2281 tatcggctgg acgaatacaa ggggtggacg tggaccggcc atggccgttt tccaaccaac
     2341 actccgggct ggtggggcgg agctcatccg ttctgtctgc tcgattacag catcgtgcac
     2401 aacggcgaga tcagctccta cggcatcaat cgccgctacc tggagatgtt cggctacaac
     2461 tgctcgatga ggaccgatac cgaggtggtc acctacctct tcgacctgat gctgaggcgg
     2521 cacggcctgc ccgtggagat cgcctgcaag gccttggcca gtcccttctg gaaagacatc
     2581 gatctgatgg gtgaggaaga caagcgactg tacacggccc tgcgctccgt ctacggctcg
     2641 gccttgctca acggcccttt cgcagtcgtc ttgggcagag gagagggcat ggtcgctctc
     2701 agcgatcgca taaagctgcg gccgctggtg gccgcacgcc acggcagtgt ggtgtacgtg
     2761 gccagtgagg agtcggccat ccgcgagatc tgcccgaggc ccgaacgcag ttggatgccg
     2821 cgcgccggcg aaccggtgat cgcgcaactc catgacggcg tgaacccggt gacctcgctg
     2881 gccgcggcgt ccgccggcgt gacggcgtag ggggcgcgcc atggccgaga tgactttcgt
     2941 cagacctgag ttccgggtgc ggatcgaccg cgaggagtgc accaagtgcg ggcgttgcgt
     3001 acaccagtgc ggttggggtg tctaccatct ggacgacgac ggacgcccca tccccgacca
     3061 cgcgaagtgc gccgcgtgcc accggtgcgt caccttctgt ccggcggacg ccatcaccat
     3121 cgagaagaat ccgctcgcgt acaaagaatc cgacagtatg tcggccgccc atcgcaaggc
     3181 catctggcgc caggctgaga ccggggggat actgcttaca ggcatgggca acgacaagcc
     3241 ctacctgcgc atcttcgatc atctgctgct ggacgcctgt caggtcacga acccgtccat
     3301 cgaccctctg cgggagccca tggagcttcg caccttcctg ggccgcaagc ccgacttcgt
     3361 cgaggtggag tgggggagcg acgtcggggc gaagccgcga ctgctcaccg agctcgaccc
     3421 gcaactgacc ctcgagacgc ccataatgtt cggcgggatg agcttcggct ccgtgtcgtt
     3481 gaacgttcat cgcgccttgg ccatggcggc caaggcttcc ggcaccttca tgaacacggg
     3541 cgagggtggc ctccacgccg acctggagcc ctaccaggac cacatcatcg tgcagtgcgc
     3601 gtcgggccgc ttcggcgtgc acaccgacta tctgcaggcc ggcgccgccg tcgagatcaa
     3661 gatcggccaa ggagccaagc cgggcatcgg cggtcacctg ccgggggaga agatcgacga
     3721 gcaggtgtcg accacccgca tgatccctca gggcacggat gcgctctctc cggcgcccca
     3781 ccacgacatc tactccatcg aggatctgcg tcaactcatc tatgccatca aggaagccac
     3841 cggatacaag ccggtgtcgg tgaagatcgc gtgcgtgcac aatgtggccg ccatcgcttc
     3901 cggagtggtg cgtgccggcg ccgacatcgt ctatctcgac ggcttccggg gcggcaccgg
     3961 cgcggcgccc accgtcatcc gcgaccatgt ggggataccc atcgagatcg ccatcgcggt
     4021 ggtggatcaa cgcctgcggg acgaaggcat ccgcaaccgg gcgtcgatcg tcgcggccgg
     4081 cggcatccgc agctcggccg acgtggccaa ggccattgcc cttggtgccg atgcctgcgg
     4141 catcggcacc ccggccctgg tggcgctggg ttgccacgtg tgccagaagt gccacaccgg
     4201 ttgctgctcg tggggcatct gcacccagcg accggagctg acgcgccgcc tcgacccgga
     4261 gtggggggcc gaacgggtct caaacctcgt gagggcctgg agccacgaga tcgccgagat
     4321 cctgggcgct ctgggagtga atgccatcga gagcatgcga ggcagccgcg aacgcctgcg
     4381 cgccctcggg ctcgaccagt cgacgatgga catgctgggc gtcaagccgg cgggcatggg
     4441 ccactgatgg ggggagaaag accgtgagcc cgtcatacga gtcagtgagg agcgtcccca
     4501 cgggccccgc ccggcgcggg cccaccggca tcgagcccgc ccgcatcacg gcgggccagc
     4561 cgggcgcaga gaggggcgag gtcgcccgag agggcgatat cgcccacatc gacgcacgcg
     4621 ggatctacta caagaccttg aacgagcaga tccgcgaaca ggtggccgtg ggcgtgacga
     4681 ccgtggtcct ggaccacgtg aacggccaac ggtacatcgg ggcgggcatc agcgccgatg
     4741 gtcttaccat agtggtgaac ggcgtccccg ggaacgacct tgccacgttc atggacgggc
     4801 ccaccgtggt ggtcaccgac aacgcccagg acggcatcgg caacacgatg aaccgcggcc
     4861 tggtagctgt ggaaggtgac gcaggcgacg tgatcggcta cggcatgcga ggcggccgca
     4921 tctacgtgcg gggcgacgtg ggatatcgag tgggaatcca catgaaggcg tacgagcgcc
     4981 acatccccgt gatcgttatc ggcggcaagg cgcgagactt cttcggcgag tacatggccg
     5041 ggggcgagat ggtcctcctg gggctggggg agaccgacga gcccgtggtc gggtcgtacg
     5101 tgggcaccgg catgcatggt ggagtcatct tcgtgcgcgg cgaggtagcg ccgcaccaac
     5161 tcggcaaaga ggtcggactt gcgtgtccca ctgctgccga tgaggcgcgt atcgaggaac
     5221 tggtgcgcga cttctgtcgg gtgcacgggc tggatgcggc tccggtcttg gcggccccgt
     5281 tcgtgaaagt agcaccgttt acgcaccgtc catacggcaa gttgtacgcc tactaggctg
     5341 ccgctcgacg cgggttcacg cggcgcttgt acaaatggat aggcaagttc agtctaacgt
     5401 tgtccaggtt ggtgttgttc ttctggcgaa tcgacggtag tcttcttgtc gttctcaacc
     5461 actgttctcc aggaagggct gtcaatggga aacggcatga cgaaggacga cgttctccgt
     5521 atcgtacgcg aacaggacat caagttcgtg cgcttctggt tcacagacgt tctcggcttc
     5581 ctcaagagct ttgcggtgac cccctccgaa cttgaaggcg ctttctccga agggatgggg
     5641 ttcgacggct cctccatcga gggtttcacg cgcatccagg agtcggacat ggtcgcgatg
     5701 cccgacccgg gcaccttcaa cgtcctcccc tggcgccgtg gctccggggg cacagctcgc
     5761 atgttctgcg acatccagat gcccgacggc aagcctttcc cgggtgatcc ccgctacatc
     5821 ttgaagcgtc tgctcaagaa ggccgccgac ctgggctaca ccatgtacct ggggccggaa
     5881 ctggagttct tctacttcaa ggacagcaag ggcacggagg tgctggatcg cggcggttac
     5941 ttcgatctca ccccactcga cgtggcgacc gatcttcggc gcgacaccat cttggcgctg
     6001 caggacctag gcatcgacgt cgagtactca caccatgagg tggcgccctc gcagcacgag
     6061 atcgatctca aatacaagga cgcgctcacc atggccgacc aggtcatgac ctaccgcttg
     6121 accgtgaagg aggtcgcgca gtcccgaggc acgtacgcga cctttatgcc gaaacccatg
     6181 tatggcgaga acggctccgg tatgcatacc caccagtccc tcttcaccgg gccgaggaac
     6241 gctttcttcg acgggaacga cgagtaccac ctttcgtcgg ccggcaaatc ctacatcgcc
     6301 ggattgctga agcacgcgcg tgagatgtgt ctggtaacca gccagtgggt gaactcctac
     6361 aagcggctcg tacccggcta tgaggcgccc atctactgct gctgggcgcg acgcaaccgg
     6421 tcggccatgg tgcgcgtgcc gatgtacaag cccggcaagg aagaagccac ccgcgtggag
     6481 atgcgcaatc ccgacccggc atgcaacccc tacttggcct tcgcggtcat gttggcggca
     6541 ggcctcaagg gcatcgaaga gggatacgaa ctggctcccg aatcgaccaa caacatctat
     6601 gagatgaccg atggcgagcg cgaagccgcg ggcatcgact ctctcccagt agacctgtat
     6661 gaggctatca atgagttcaa gggttcggcg ctcatgcggg aagctctcgg ggaccacgtg
     6721 ttcgagtatc tcatccgcaa caagcaggcc gagtgggacg agtacaaagc catcgtgacg
     6781 ccctatgagt tggaccgcta cctggccgtt ctctagagag ggaggatcgc ctgcgacgcc
     6841 tcacgacgca ggcgtcgccg aggagaagtg gcagggggcg cccggcgacc acgccgggcg
     6901 cccccttctc gctgaggacc gcgccgaccc ctcggccccc ggggggatac aatccttctc
     6961 atgaacgatc acggtaatga cgcgcccgag cacggcccgc tctttctcat cgacgggaac
     7021 aacctcgcct accgtgcctt cttcgcgcta cccgagacga tttccacgtc ggccggcttt
     7081 cccaccaatg cgctgtacgg cttctgctcc atgatgatca aggtgctcgc ggagttcgcg
     7141 ccagaggtgg tcatcgtggc gtgggactcg cgggagaaga ctttccgtca tgaggacttc
     7201 gaggagtaca aggcccaccg caagccgatg ccggacctct tgcgtgaaca gtggcctcac
     7261 ttgcgagaac tgtcggaggc gttcgggttc gtcaatctga acgtccccgg ctatgagggc
     7321 gacgacatcc tgggcaccct ggcgcgtcag ggagacgccg agggcaggca ggtcgtcatc
     7381 gtgaccggcg accgggacgc gctgcagttg gtcacggcga acgtcacagt gatgtcgaac
     7441 acgcgtggca tcaccgatgt gaagctctac gaccctcatg cggtcgagga gcgttttggc
     7501 gttcccccga gcctcatccc cgacctgatc ggcctgaagg gcgactcttc cgacaacatc
     7561 cccggagtgc cgggtatcgg tgagaagacc gcagctttgc tacttcagga gtacggatcg
     7621 ctcgaggaag tgctggcgca cgccggcgag gtgaaggcgc ccaaacgcag ggagttgctc
     7681 ctgaagcatg ccgacgacgc gcgtatgtcc aaggagttgg ctcggctcga tcgggaggtg
     7741 cccatcgaca tccacgctgc ggaagtggcg ccccaccact tggatcgtgc ccgtctggcc
     7801 gagttgttgc accgtttcga gttctcctcg ctgctcgacc gactcggctc ggaacccctc
     7861 gcacgggtgg tggacatccc ggcgccggcc cgggcccacg gccccgggct tcggatcgag
     7921 caggtggggc tccgcgaact cgcgcgcgaa ttggcggttc ccggtcggat cggactggcc
     7981 ggcgcccgcc aagacggcct gagtcgactg tggctggcgc ggcggggcgc cgccgatggc
     8041 acggtccccg cgggcccgtc ggtcgacccc gcgccgcccg ccgacgcggg cgccacgccc
     8101 ctcgccgacg ccacgccgcc cgccgacgcg acggccttcg ccctcgactt ctccgagacc
     8161 gaatccgacg cacttcgcgc gctgctggct cgaagtgagc ccgcctgtca cgacttcaag
     8221 agcgaccccc tgctccccgg tcttctccgg tcggtcagtc acgacacgat ggtggcggcg
     8281 tatctcctcg cccccggaag acgctcatac gagctctccg acctcgcggt gcaggcaggg
     8341 gtatcgtggc tcgacgtgga ggagacgtcg ggcttggacg ggcccgacgg acacgcgacc
     8401 cgggcgctcg cggcccttga cgtttctcga ttccaggaga gagctctgcg cgagacaggc
     8461 atgtgggact tgttccgcga cgtggaactg cctctctcca gggcgcttct cgacatggag
     8521 gcagtcggta tccacctgga ctgctatcga ctcggcgaga tcacggccaa ggtcgaggac
     8581 cagctggatg ggctggaggc ccaggcgtac gaggcggccg gagaggagtt caatcttggt
     8641 tcgccgcaac aactggccgg cgtcctgttc gagaagctcg gtctacccag acagcggaag
     8701 accaagacgg gctactccac cgacgcgcac acgctcgacg ccctgcgcga ccttcaccct
     8761 gtcatcgagc tgatagagac ccatcgcgaa ctcacgaagc tgctctccac ctacctgctg
     8821 gccttgccca gcgcggtgga tccggcgacg ggtaggctcc atacgacctt ccatcaggca
     8881 gtggctgcga caggacgtct ctcttcgagc gatccgaacc tgcagaacat cccggttcgc
     8941 accgacctag gcgctcggat acgcggatgc ttcaccgccg aggaaggccg ggtgctcgtg
     9001 gtagcagact actcccagat cgagctgcgc atcatggcgc acctgtcggg cgagcccacg
     9061 ctactggacg ccttccggcg cggagaagac atccacacgc gcacggccgc ggaagtgttc
     9121 gacctgcccg aggaagaggt cgacgccaca caccgccggt atgcgaaggc ggtgaacttc
     9181 ggcatcatgt acggcatctc cgcgttcggg ctggcccggc aactcaagat cccccagaac
     9241 gtggcgggag agtacatcga gcgctattcc gcccgcatgc cccacgtccg cgcgttcatc
     9301 gaagcgacca tctccgacgc gtcggcccag ggtttcgtgg ccaccatcat gggccgtcgt
     9361 cggccgatcc ctgagctccg ctccggcaac cagcagaccc gccaactcgg ggagcgcttg
     9421 gcggtgaact cggtcatcca gggcagcgcc gccgacatca tcaaagtcgc gatgcttcgc
     9481 tgccgcgaca ggcttgcgcg agaatctaga gagacgcgtc tcgtcctgca ggtgcacgac
     9541 gaactcatct tcgagtgccc cctcgcggcc ggacggtcgg tcgcgcggat ggtgcgcgag
     9601 gagatggtgg gctcgtgggt cgtcgacccg ccgctgatcg tggacgtggg cgtcggcgag
     9661 gactggctgt cggccaagta gttggcgggc ggccgagggc gggcctcgac cgctgggccg
     9721 gtcatcgcgc acgtgttgta tttggcgtcc ccctcggtat actaacggac agcgtaagcc
     9781 aggtttccgg atggcgcgcg cattttttgt ctgcgaggaa ggaaaaaagg actacgatgc
     9841 cagatcaact gaccgacgag aacgcacgga ccctgcaggg cggccaagac ctcatgatcg
     9901 aggtcgacgg cgagatgatc cccaactacg acgcgacgat ggtgaccttc gaggaaggtg
     9961 acatcgtcac cggcacggtg gtccgtgtcg acaaggacga ggtccttgtt gacatcggct
    10021 acaagagtga aggcgtggtg cccgtcaacg aactgtccat caagaagtcg gtggacccga
    10081 gtgacgaggt gacggttggg gatgtgatcg acgctctcgt cctcacgaaa gaggatgcgg
    10141 acggtcggtt gatcctttcg aagaagcgcg cgaggttcga gcgtgcgtgg cagcgcatcg
    10201 agaaggcggc cgaagagggc acgccggttg aaggtacggt catcgaggtg gtcaagggcg
    10261 gactcatcct cgacctcgga gtgcgtggct tcctgcccgc ctccctcgtc gacatccgcc
    10321 gcgtggccga cctggacgag ttcgttgggc agccgttgct gtgccgcgtg atcgagttga
    10381 accgcttccg caacaacgtg gtgctcagtc gtcgcgcggt gctggaagaa gagcgacgcg
    10441 gcgtgcgcga gcagatactc gaccgcatga acatgggcga cttcgtcacg ggcacgatct
    10501 cgaacatcgt cgacttcggt gccttcgtcg atctgaacgg catcgacggc ctgatccaca
    10561 tctcggagct ctcttggagc cacgtgaacc atccgtcgga agtgctcacc atcaatcaag
    10621 aggtcaccgt caaggtcctc gacatcgacc gtgaccgcca acggatctcc ctgggcctga
    10681 agcagacgca gaaggacccg tggcaagagg tgttcgagaa ccggcaggtg aacgagatcg
    10741 ttcacggccg ggtgacgaag ctcgtatctt tcggggcttt cgtcgagatc gaagagggcg
    10801 tcgagggtct cattcacatc tccgagttgg cccagcatca cgtggaagat ccgtcggagg
    10861 ttcttcgcaa cgggatggaa gtcaacgtca agatcatcga gatcgatccg gaccgccgcc
    10921 gcctgtcgct gagtttgaag cgtctcgagc ccgaataccg catccacgag gatgccatgg
    10981 cgcaggagat cgaggctgaa gaggccgtgg ccgtagaggc cgccgaagca ggcgccgagg
    11041 tcgatgaggg cgctgggatc gaaagtacgg ctgttgccga aggcgacgct agcacggaag
    11101 cggatggcgg agccgaggca gaacctgaag cgcacgcaga agcggaagcg gaagcagcgg
    11161 cggaagccga cgcggaagca gaacctgaag ccgacgcgga agcagagacg gcagccgaag
    11221 ccgaggtcga agccgaggcg ggagaggaag cggccccaga gtcagaggcg gtggtcgagt
    11281 ctgaggcgca accggaggct gtcatcgaca cccccgacga ggcggctagc gaggacgcca
    11341 ccgaggctgg tgccgacacc gccagtgacg ccggcgccga gaccctcacc ggctccattg
    11401 aagatgcggg cgaagatgtg ggcgaagatg cgccggtcga agaagcggtg gtggacgaat
    11461 ccgacgctct cgacgccgac agcctgaagc tttcggacga ggtgttcgag gcggagtgac
    11521 ccggctcgat attgagtgag tcgtagcaga gggcgccccc gggcgccctc tttcttgtgg
    11581 cgtggacgcc gatgggggtg aggtcacggg tttccgtcac gggcgggcgc tacaatagga
    11641 cgatgaaagt ctgccccact ccagcgctcg agtctcgagc cgaccccatg tccgaacgcc
    11701 ggctgcaggc cgacggccgc gcggcgatcg ggctcacggg cgggttggcg tcgggcaaga
    11761 gcagcgcgtt ggagatgttc gcccggttag gggcgagggt catctccgca gatgccatcg
    11821 tccatggact gtacgagttg gcagaggtga ggtccgcttt gcgcgcgcgg ttcggcgacg
    11881 gcatcttcga cacaagcggt gcggtggatc gtcgggccct ctcgtcgcgc ttgagtcgtc
    11941 gaggggacga tctggcggcc gttgaatcga tcgtgcaccc gcacgtggaa ggcgaactga
    12001 gggcgttcgt ggctcacggg ccccgcggga gcgtgaacgt atgcgaggtc ccgctgctgt
    12061 tcgagggtgg actgcagggg ttgttcgact tggtgttgac catcgaggcc ccggcggcgg
    12121 tgcgtcggga gcgcgcctcg tcgcgcatga ctccagatgt gttcgaatgg ctcgatgcac
    12181 ggcaggcggg ggaagaagct cgctccgccg gtgcagacct tgcgtatgcg aacaccggct
    12241 cacctgagga gctgcgcacg ttcgtgacgg gcgtctacga gcgggcgcaa gcgatggcga
    12301 ccgggcgcgc cgaggggtca catgcgcgcg cgtaacgcac gaggctcggc gtcgcgcggt
    12361 cgctctgcgg acaggcgatc tcggttcgcg gcgaggggga tccgcttgtt cctgatcgta
    12421 ttggggctcg cgctcctcgt ggtgggcctg gtcgcctcag gtgtcggcgg ggagctgaag
    12481 acacgtgtgg aaagccggtt gtatcccctg aaattcgagg agcatattct tcgtgctgcg
    12541 acggcgtacg gggtggatcc ctacctggtc gccgccgtgg cgaaagctga gagtggcttc
    12601 gagccggggg ctgtgtcgcc ggtgggcgcc gtggggctca tgcagatcat gccggagact
    12661 gccgtgtgga tccagcagcg accggactgg aagggcgggg cggtgcccga cctcactgat
    12721 cccgccgcca acttggagat gggcgcctac taccttgcgt atctcacgca gttgttcgcc
    12781 ggagacctga ggtcaaccct ggccgcctac aacgccggcc agggtacggt gaccggctgg
    12841 cttacgctat cgggcagcgg gacgggtctg cagtcgggcg acatcctgtt cgccgagacg
    12901 cgggagttcg tcgcacgagt ggagaaactg cgcgcacgct tcctccgggc tcacccggga
    12961 gcgttcgtca cgtgatgcgg gcacccggcg cagggttggc tc
//
LOCUS       DASRMJ010000323        21688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_174754, whole genome shotgun sequence.
ACCESSION   DASRMJ010000323 DASRMJ010000000
VERSION     DASRMJ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 21688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21688
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174754"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFD74_08870"
     CDS             complement(<1..434)
                     /locus_tag="VFD74_08870"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255967.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZK49693.1"
                     /translation="MSDPARFAPAGRGLRGVVAVPGDKSISHRALLLGAVSRGPAVVR
                     GFLPSADTLATLEAVRALGVDVEVRDSSELVVHGVGWEGLSEPEDVIDVRNAGTLLRL
                     LPGLVAGRPFLTVLTGDASIRKRPMARVLEPLSAMGACAWGRG"
     gene            complement(427..1593)
                     /locus_tag="VFD74_08875"
     CDS             complement(427..1593)
                     /locus_tag="VFD74_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZK49694.1"
                     /translation="MARKVDRLTQMAPTRETRPVRTLAIIGVGLMGGSLALAARHKGQ
                     VDVVRGFDSDPRCLDDALAGGVITRRCASVAEAAAGADLVVVCTPVRGILDLVEQAAL
                     ANPPPRMISDIGSTKATLLNRLSARARPLFIGGHPICGAETSGVRFARPDLFEGATYF
                     LCPPDEVAPEAFEAFHSWVAALGARPVAIGADVHDRIMALVSHVPHVLANVLMSEVGG
                     FEASGRRALYSVGPSFKDLTRVAGANPAMWTDIFIENREALISSLKSVAADVMLLCEH
                     LRDGDEKSVFKGIDSAASYRRELLQFEDILPETLHRITVRIPDEPGVLSRVMTSLGNA
                     NINIEDLTLHHFSRSAGGDLLLYVSGAEEAGIACDLVEALGFPAIMEFAGDPGV"
     gene            complement(1569..2615)
                     /gene="aroF"
                     /locus_tag="VFD74_08880"
     CDS             complement(1569..2615)
                     /gene="aroF"
                     /locus_tag="VFD74_08880"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZK49695.1"
                     /translation="MSMLEAGGIEMMIVMQEGATQREVEHVLERLGEAGARGHVSTSD
                     VVTVIGVIGDREVIAGIPLEAFVGVDKVLPILKPYKLVSREFRRSDTVIEVGGNKIGG
                     SHVALIAGPCSVESREQLLSSARYVKQSGATMLRGGAYKPRTSPYAFQGLGLEALQIL
                     AEARDETGLPVVTELMDQRHLEAVVEYADVIQIGARNMQNFYLLSAVGEVDRPILLKR
                     GLSATIDELLMAAEYIANAGNTQVILCERGIRTFETATRNTLDLSAVPVIKQRSHLPV
                     VVDPSHATGKVDLVEPLSLAALAAGADGLLIESHPAPEAALCDGGQSLNPKEFARTAA
                     RLRELAAWLGKSIG"
     gene            complement(2612..3718)
                     /gene="hisC"
                     /locus_tag="VFD74_08885"
     CDS             complement(2612..3718)
                     /gene="hisC"
                     /locus_tag="VFD74_08885"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZK49696.1"
                     /translation="MRLTKALEALTAYKAGPPLESLRERYGLNRVVMLAANECPEGPF
                     PEVIEALTTALPSLNRYPSGGCTELRSALAERHGVPGERLVFGNGSCELLILLGEALL
                     EPGEHMVFADPSFVVYRTVALKREASFDAVPLRDHVHDLDAMSAAIGPETRLVIVCEP
                     NNPTGTYVGPQALRRFVESVPRETLVVLDEAYTEYVTHPAHEDTIPWLDDYDNLAVLR
                     TFSKIYGLAGLRIGYGVFPPRLAEALDKLRQPYNVTTLAEVAALEALRHPTRLEERRA
                     HVRRERVRMSQELERLGVSTVPSEANFMLANVEGLRVPAGEVPRALCERGVVVRSGSG
                     IGCPGWMRVTVGTVEENDVFLGIMGDMAGKEWPR"
     gene            complement(3760..4473)
                     /locus_tag="VFD74_08890"
     CDS             complement(3760..4473)
                     /locus_tag="VFD74_08890"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZK49697.1"
                     /translation="MIQRLQRVLASAGLGSRRSCEDLIREGRVTVDGRTAELGSSVDP
                     SLQAVLVDGVPIHRETLEYWLLNKPKGVVSTAKDPQGRRTVVDCVPASVRIFPVGRLD
                     ADTTGALLLTNDGALAHRLLHPSYGVEKEYRVVVVGRVTEVTARRLAAGIDLEDGVTA
                     PARVVVMSSRQTRSELGVRIHEGRNRQIRRMLEAVGHPVVELHRVGFGPLSDAAMVSG
                     ASRHLEASEIEGLRRLVDL"
     gene            complement(4470..5129)
                     /gene="scpB"
                     /locus_tag="VFD74_08895"
     CDS             complement(4470..5129)
                     /gene="scpB"
                     /locus_tag="VFD74_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZK49698.1"
                     /translation="MIDADPLGADSSGPAAPTTERAPQPAAHLTRELEALLFAAGRPL
                     DLDSLREAASYHEPMEKAAVAAALEVLESRYPVGGDKGFELARVGGGWLFRTNAACEL
                     ALARLFDAGEELRLSPAATETLAIVAYLQPVSRPQIAEIRGVNSDSALHTLLDRDLVR
                     EVGRRDAPGAAVLYGTTTRFLVAFDLADIGSLPPIEEFEVGDDQREELKRRLGLVVAP
                     G"
     gene            complement(5126..5875)
                     /locus_tag="VFD74_08900"
     CDS             complement(5126..5875)
                     /locus_tag="VFD74_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZK49699.1"
                     /translation="MRVLDLDLDVYQGPFDLLFTLILREEVDIFEVPIVEVILAYVEE
                     LAATDAVDWEALTEFLVLISSLLELKSRVLLPGEEVGIEDADAELSKDVLLERLLTYR
                     RYKMAAASLAEVRERQRGRLVREPARAPRRALAPDEEIRGSQAPASLTRALGRLLESH
                     RLPDTSHVAVLRIGLNRQIAAIRRLLRERGSFSFEEEFGTEEPMVQAVSLLALLGLVS
                     RGDVKVAQSSHFGDITVKHVDIQRVALRGTA"
     gene            complement(6054..6710)
                     /locus_tag="VFD74_08905"
     CDS             complement(6054..6710)
                     /locus_tag="VFD74_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZK49700.1"
                     /translation="MISDLLGFALLMPVLLLSMVAHEVAHGAVAYRLGDPTAKAHGRL
                     TANPLKHLDPLGTMMFAITYFAGGFIFGWAKPVPVNAGYFRDHQRGMMLVGLAGPFTN
                     FLIAVVLAGILNPLAPAVSSVQALYGGSILLRALFLGFQVNVVLGIFNLVPIPPLDGS
                     RVIAGFMSPSTYARWAGLDRYGMLFILLIFFVLQRPFFSLLGGGFNLVSRLLLPAYGF
                     "
     gene            complement(6745..9456)
                     /locus_tag="VFD74_08910"
     CDS             complement(6745..9456)
                     /locus_tag="VFD74_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK49701.1"
                     /translation="MSQSSADIKVADIITTHQNVDFDAFAAAVGASRLFPEARIVFAG
                     SLNRNVREFVALHGEDLPIVGFRSLDLASVRRLIVVDTSDCARLAELGSLCGRPDVEV
                     VVFDHHEEEAPERPSFVEGENWVLSTDGAQATSMVHILRERDVPISPLEATIFALGIH
                     EDTGSLTYPRSTVRDAEMLALCLRLGASQALIEHYLHNPLTAEQRAILLRMVDEVRVV
                     EVWGQEIHVVAMSHGEYVDGLSVIAHKLMDLLNSDVLLQVVEMEGRVFVTARSRSAVV
                     DVAGLLASVGGGGHAQAASAVLRDRSAENVLDGLLGRLAETVPTLPTAGDIMSHPVRF
                     IDAETPVDEALLTAQRYGHSGISVKEAGFVVGIVARRDLDKAVRHGLGHAPVKGVMTR
                     NVVFAPESATVDEVRRLMGSTNVGRLPILTDAAYERARELGRVEVEGVVGIATRTDVL
                     AALQVGSVPDRELGAPAVCAIRSLTDLPAFGKLFRAVSALSEDFPGVYLVGGFVRDLL
                     LEKPNADIDIAVDGDGIDFARRLAAKMGGRVRTHQKFQTAVVLVSVDVVGDGEASPLV
                     SSEPFHVDVATTRTEFYDYPAALPSVEHASIRQDLFRRDFSINAMAVSLKGQEFGEVL
                     DFFGGLKDLQEGVIRVLHNLSFIEDPTRIFRAVRYENRYGFRMDEQTRSLARSCVEMR
                     LVGDLSSARLRDELVALLGEKDVDWTLARLFELGVARQVHPKLATGERTVRLIQVLDG
                     LVEEFGVASDVVSWRLRLAAITRNMSHEELFIWLEKLRLRHADRQVVRDSVILGPHLV
                     EQLADEARGDWDVFRLLRKVPIESLVFALGRTPSGPGQDRLRQYLEVLRKRSLDVTGA
                     DIMALGQPSGSGVGRVLAQLTRLRIEGDLESREEQLAAAAALVRRTL"
     gene            complement(9575..10342)
                     /locus_tag="VFD74_08915"
     CDS             complement(9575..10342)
                     /locus_tag="VFD74_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK49702.1"
                     /translation="MRIRSRRDSEPEYEDGTVPSYWRRRRLTAGRILLNLVVVAAFAA
                     AVAAVYMLVQNSGIIDRTSDETTSSTATAPTDYVLGGPIDEAVTTGLMSLSGPSRFGP
                     TESVSRGEFAGVVVRTMGWDVAADEAQDFADVVGDPEVLDEADYIAAVAARAVMRGTG
                     GVPAAFMPDQPVMVGHVLVVFARAAGDALPEATEVDPSLLQVRVDDETLSAYRRLAAA
                     GILADVSLDSADETLSNDADRQQVAVMAVNLKHFLAQ"
     gene            complement(10342..11652)
                     /locus_tag="VFD74_08920"
     CDS             complement(10342..11652)
                     /locus_tag="VFD74_08920"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HZK49703.1"
                     /translation="MRAVDLIAAKRSGAEHSDGEIRWLVESYVAGAIPDYQVAAWLMA
                     VCFAGLTRQETFVLTDAMVRSGEVVDLSDVGGIVADKHSTGGVGDKVSLVLGPLVASC
                     GVPFAKMSGRGLGHTGGTIDKLESIPGFRTELTADEFRRQVAEVGVAIVGQAPGLVPA
                     DKLLYALRDVTATVEQESLIASSVMSKKIAAGATAIVLDVKVGGGAFLHDMEKARRVA
                     RTMIDLGRDAGRDVRAILSDMDEPLGFAVGNALEVREALEVLSGGGPTDLREVVLVLA
                     GHLLVMARKDDTLERARARAAAHLDDGSAREAARRWLAAQGADPAAVDGTGLPQAPAV
                     LRVRATQGGHVQEVAALAIAEACLALGAGRESKGAAIEHSVGVVLKVKGGAEVDRGDV
                     MAVIHARTPQEAEAVEALVASAFHVGTGRVEPRRLILDEIPEGV"
     gene            complement(11649..13694)
                     /locus_tag="VFD74_08925"
     CDS             complement(11649..13694)
                     /locus_tag="VFD74_08925"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HZK49704.1"
                     /translation="MARFIVMILDGVGAGALPDAASYGDAGSNTLGNLAKLVPLRVPN
                     LAALGLGNALPLTGVAPAAAPRALPGVLAEKSAGKDTTAGHWEHMGVVTTSPFPTYPF
                     GFPEEVLGPFEKAIGRKTLGNVAASGTEIIAELGGLHVASGSPIVYTSADSVFQIAAH
                     VDVIPLEELYRYCQIARDLLTGPHAVGRVIARPFAGPAGAYVRTKDRKDFSLEPPSDT
                     YLDLLTAAGVPVTGVGKIHQIFAGRGVGRQIKVGSNDENMERLFELLDDGDDGLIFTN
                     LVDFDMVWGHRNDIEGFAEGLAAVDRRLPALLEKLRPSDRLLITADHGVDPTTVSTDH
                     SREYVPLLYYPRPAGAPASAYIGSMSDTGASAYFHLIGGDPVLGGIPVQRLRPEYGWR
                     AYPSIPVARRGGGKCVRSAPQPSGAGGEVPVSAAHWLEQRYGAPPDVAIVLGSGLDAV
                     ADNMLELDETPYSDIPGWSVGSVPGHSGRLAVGRLEGCRVAILRGRVHGYEGFDIGMV
                     QLPVRTLGCWGVRNIVITNASGGLGASPAPGQVAVTERILDFQLPLADGEPEELEATD
                     PRVLELLKGAPPQSLPVVTYAALPGPQYETGLEVAVLRRLGADAVGMSTAQEVRAARE
                     AGMTVTVLTVVTNAAGETGADGEAVHREVVESSTGAATKVAAVVALLLAGCAREEAG"
     gene            complement(13694..14617)
                     /locus_tag="VFD74_08930"
     CDS             complement(13694..14617)
                     /locus_tag="VFD74_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HZK49705.1"
                     /translation="MGAPSAAAPLPPAVADYLTCLRLEHGLADHTVASYRRDITQLTA
                     HLAGAGIGVEEALTDDLRGFLAAGDWKPSTRARKTAAIRSLYSHLVRVGALAADPATG
                     LASPRMSPGLPKPLTVEQVDRLLTRPPATPAGLRDRALLEVLYGAGLRASEGVGLRLQ
                     DLDLEVGFVRTMGKGSKERVVPLGRKAVEAVGIYVARGRPLLGLPGGLKPPQLFLNTR
                     GKGLSRQGLHLIVKKYAALAGLSSDVSAHTLRHSFATHLVEGGADLRAVQEMLGHADL
                     ATTQIYTHLSTAHLRRVYDDAHPRARSARTE"
     gene            complement(14702..15826)
                     /gene="ald"
                     /locus_tag="VFD74_08935"
     CDS             complement(14702..15826)
                     /gene="ald"
                     /locus_tag="VFD74_08935"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011704256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZK49706.1"
                     /translation="MRVGVLKELKADEYRVALTPAGARELTSRGHTVLVETLAGEGSA
                     FGDEAYVASGAQIAESAEAVFSGAELILKVKEPLEQECLRLHSGHVLFTFLHLAPNAE
                     LTRGLVDSGCVSVAYETVGQDSGHLPLLAPMSEVAGRLAPQAAAYFLERMNGGKGKLL
                     AGVTGVAAAKVVILGAGIAGSNAALVASGMRARVTILDVKVERLAEVERMVPGVEALM
                     SNQLTIEEQVERADVVIGAVLVPGARTPQLVSEDLVKAMQAGSVVVDISVDQGGCIAT
                     SHMTTHSSPTFVVDEVVHYCVGNMAGAVPITSTLALTNATLPYIMKVAEQGLVAAARS
                     DRALARGINVMEGKVTNSQVAKATGNRYFPLESLLPIEFS"
     gene            complement(15839..16459)
                     /locus_tag="VFD74_08940"
     CDS             complement(15839..16459)
                     /locus_tag="VFD74_08940"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZK49707.1"
                     /translation="MGEGRPDGVTAAGWVDSSGMQDQAQAEAPPEILSSRTVYSGAVV
                     SLRVDEVRVATGTVTREVVEHPGAVVMAALDERERVVLVRQFRHAVGRELLEFPAGGL
                     EPGEDPGAAARRELREEAGLVAHEWVSLGSFFSSPGFLHEELHAFLARGLVSTVQDLD
                     VEEDIEVVWVRLEDLVRGVPAVTDAKTLATLLLLQRHLARDEDQGA"
     gene            complement(16461..17627)
                     /locus_tag="VFD74_08945"
     CDS             complement(16461..17627)
                     /locus_tag="VFD74_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZK49708.1"
                     /translation="MTDAPSNEAGGLVALFMELASISSPSRRERAAADLVTARLRGLG
                     LEVVEDDAAQRVGGDTGNLRCVVRGRGASPSVCLAAHLDTVEPSGPIRPALNDGVIRS
                     SSGTILGADNKAAVAALVTVTEGLVVGGDAFPTFELIFSVAEEVGVLGSRFLAPDLPA
                     APIAAVFDSSGPVGGIVVRAPSQQTIRATFRGQAAHAGLEPENGRSAIQAAAHAISLM
                     ELGRLGPETTANVGMIEGGVAQNIVPESCRVVAECRSLDDEMLAATSSRMIDALQLGA
                     ARVGTDLDLDVVTEYRAFRLDESSAVVGLARRAIEALGLPARTHASGGGSDANVFNGR
                     GTPTVNFDCGMSRVHTAEESMDVAQLVLLGELVRNVIVMAEPAGGGRSGSEGVV"
     gene            complement(17624..19258)
                     /locus_tag="VFD74_08950"
     CDS             complement(17624..19258)
                     /locus_tag="VFD74_08950"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZK49709.1"
                     /translation="MAKHVFVTGGVVSSLGKGITAASLGRLLKSRGLRVVIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFVDENLTRESNITSGSVYYNVIRKE
                     RRGDYLGGTVQVIPHVTNEIKERISRVSRNLPVDVVITEIGGTVGDIESLPFLEAIRQ
                     FRNDIGRQNVLYIHVTLIPYIGVAGELKTKPTQHSVNELREIGIQPDIIVCRSDRPLS
                     RELKDKIALFTDVEPRAVISAIDAANIYFVPLNLRDEGLDDLVVAKLGLECGPVDLSE
                     WEALVARIRARSGIVRIALVGKYVQLQDAYLSVVEALRHAALHHGRDVEVVWVDAEGL
                     RPEEIRSQLETADGILVPGGFGVRGIEGKILAIEWAREKKVPFLGICLGMQVAVAEFA
                     RHVAGMNDANSFEFDPETPFPVIDLLPEQKDIEDMGGTMRLGASPVKVIPGTKAFAAY
                     GEEVVYERHRHRYEVNNGLRARLVEAGLVISGVTSDDRLVEIIELEDHPWFVASQFHP
                     EFKSRPTRPQPLFRDFVGAAIECSCGVDKGADGVSE"
     gene            complement(19323..21026)
                     /gene="recN"
                     /locus_tag="VFD74_08955"
     CDS             complement(19323..21026)
                     /gene="recN"
                     /locus_tag="VFD74_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZK49710.1"
                     /translation="MLTSLSIKDYVLIEDASLEFGSGLTVLSGETGAGKTLLTQALGL
                     LLGERAGDGLVSELSQEALIQGVLELSESQLAEVPADTRDLLDLEAGELVVTRRLSRV
                     GKNRCFLNGVAVPLGVLSEALGDLISFSGQHEHRRLLKPSHQLQVLDAFAGSDQTRDR
                     QEYRACWVEARSGEARLDALRRGVAERRRESELLRFQVQELEAAALEVCEERELEVEQ
                     RRLSRIEEMARACAAAAGCLRSDDVGADVGGLVSAARSGLTPFLGLDPRLDDSLASLS
                     EADGLIDDVARTLHTLMGSLEPDPARLNAVDERLRVYSDMAKKYEGTTEAAIAFLAEA
                     SERLRSLETSEQDLSEMTRHVAERRGRCLELAAELSKRRVEAAARLQAAVRPHLVDLG
                     MAEGTAPVSVESRQGWDALDADGADSVEFLLAANVGVEARSLARTASGGELSRVLLAI
                     KTALRGCETAETVVFDEIDAGVGGLTATAVGAKLRETSRNCQVIVVTHLAQVAAFADR
                     HYLIRKEVAAGRSVTSLQVLDGQGSLEELARMLGGAPGDQGALALARSLKHRAEVGLI
                     D"
     gene            complement(21040..>21688)
                     /locus_tag="VFD74_08960"
     CDS             complement(21040..>21688)
                     /locus_tag="VFD74_08960"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZK49711.1"
                     /translation="CLVFGGDGTMLRALRMTRDLGVPVAGVNLGRVGYLATVGRDRIV
                     EDLPRLLAGEFVTQPLLGLSTVCDGVTLRAVNDFVVGRGREGGVCRISYALNGNRLFD
                     VRCDALVVATPAGSTAYNLSVGGPVVGVGVEGFVVSYVAPHALGVRSVVAAAGDVLTL
                     TNESTHSAVEVLSDGEHVAELAPLANLEVGTIPALAALALLPGTDFYQHFAERFV"
BASE COUNT         3850 a         7729 c         6655 g         3454 t
ORIGIN      
        1 cctctccccc acgcgcaggc gcccatggcg gacagcggtt ccagcaccct cgccatcggc
       61 cgcttacgga tgctcgcatc gccggtgagc accgtgagga aaggcctgcc ggcgaccagg
      121 ccgggcagta ggcgcagaag ggtgccggcg ttccgcacgt cgataacgtc ttcgggctcg
      181 ctcaacccct cccagccgac tccatgcacc accagttcgg aggagtccct cacctccacg
      241 tcgaccccaa gcgctcgcac agcctcgagc gtcgcgagcg tgtcagcgga aggcaggaat
      301 ccgcgcacca ccgcggggcc gcgactcacc gcgcccagaa gaagggcgcg gtgcgagatc
      361 gacttgtcgc ccggcacagc gacgactcct cgaagccccc ggccggcggg agcgaaccga
      421 gcgggatcag acaccaggat ctccggcgaa ctccatgatt gccgggaatc cgagcgcttc
      481 gaccaggtcg caggctatgc ctgcctcttc cgctccgctc acatatagca gcagatcccc
      541 tcccgcactg cggctgaagt ggtgcagtgt gaggtcctcg atgttgatgt tggcgttgcc
      601 cagggaggtc atcacgcggg agagaacccc cggctcgtca gggatgcgta cggtgatgcg
      661 gtgcagagtc tcgggcagga tgtcctcgaa ttggagcaac tcccggcggt aggaagccgc
      721 cgagtctatc cccttgaaca cgctcttctc gtcgccatcc cgcaggtgct cgcacaagag
      781 catgacatcg gccgccacgc tcttgagcga cgagatgagc gcctccctgt tctcgatgaa
      841 gatgtccgtc cacatcgccg ggttcgcccc cgccacgcgg gtgaggtctt tgaagctcgg
      901 gcctaccgag tagagggcac gccggcccga cgcctcgaaa ccaccgacct cggacatcag
      961 cacgttcgcc agcacgtgag gcacgtggct caccagtgcc attatgcgat cgtggacatc
     1021 ggctccgatg gcgaccggcc gcgctccgag tgcggcgacc caactgtgga aggcctcgaa
     1081 cgcctccgga gcaacctcgt cgggagggca caggaagtac gtggcgccct cgaacaggtc
     1141 gggtctcgcg aaccgcactc cgctggtctc cgccccgcag atcgggtgac cgccgatgaa
     1201 cagcggccgc gctcgggccg acagcctgtt caggagcgtc gccttcgtgc tgccgatgtc
     1261 gctgatcatt cgcggcggcg ggttggcaag cgcggcttgc tccacgagat caaggatgcc
     1321 ccgcactggg gtgcacacca cgaccagatc ggcgcctgcg gccgcttcgg ccaccgacgc
     1381 gcagcgccgt gtgatgaccc cgcccgccag agcgtcgtcc aggcaccggg ggtcgctgtc
     1441 gaagccccgg accacatcca cctgcccctt gtgccgggcg gcaagcgcca agctaccgcc
     1501 catgagacca accccgatga tggcaagggt gcgtaccggg cgggtctccc tcgtgggtgc
     1561 catctgggtc agccgatcga ctttccgagc catgccgcca actctcgcag ccgggccgcg
     1621 gtgcgcgcga actccttcgg attgagcgac tgcccgccgt cgcagagcgc cgcctccggt
     1681 gcagggtgcg attcgatgag aagtccatcg gctccggcgg ccaaagccgc cagggagagg
     1741 ggctcaacca gatcgacctt cccggtcgcg tggctcggat cgaccaccac gggcaggtgc
     1801 gagcgctgct tgatgactgg gacagccgac aggtcgaggg tattgcgggt ggcggtctcg
     1861 aaggtcctga tgcctcgttc gcacaggatg acttgagtgt tgcccgcgtt cgcgatgtac
     1921 tccgcggcca tgaggagctc atcgatggtg gcggagaggc cgcgcttcaa caggatgggt
     1981 cggtcgacct cacccacggc cgacaacagg tagaagttct gcatgttgcg agccccgatc
     2041 tggatgacgt cggcgtactc caccaccgcc tccaggtggc gctgatccat gagctcggtg
     2101 accaccggca gccccgtctc gtcgcgagcc tcggccagga tctgcagcgc ctcgagtccg
     2161 agtccctgga aggcgtaggg ggacgttcgc ggcttgtacg ctccgccgcg gagcatggtc
     2221 gcaccagatt gcttcacata ccgtgccgac gacagaagct gttctcggct ctcgaccgag
     2281 cacggccccg ctatcagcgc cacgtgagaa ccgccgatct tgttcccacc cacttcgatg
     2341 acggtgtcgc tgcgcctgaa ttcgcggctc acgagcttgt agggcttaag gatgggcagc
     2401 accttatcca cacctacaaa ggcttcgagc gggatgccgg cgatcacttc gcggtcgccg
     2461 atcacgccga tcactgtcac gacgtcgctg gtagacacgt ggcctcgcgc gcccgcttcc
     2521 cccaagcgct ccagaacgtg ctccacctcc cgttgagtcg cgccctcctg catcacgatc
     2581 atcatctcga tgccgccggc ctcgagcatg ctcatctggg ccattccttt ccagccatat
     2641 ctcccatgat gccgagaaag acatcgttct cctcgaccgt ccctaccgtc acgcgcatcc
     2701 agcccgggca cccgatccct gatcccgacc gaaccaccac tccacgttca cagagcgccc
     2761 gcggcacttc cccggccggg acccgcaacc cttcaacgtt ggccagcatg aagttcgcct
     2821 cgctggggac ggtcgacacc cccagccgct ccaactcctg agacatccgg acgcgctctc
     2881 tgcgcacgtg cgctcggcgc tcctcaagcc gtgtgggatg ccgcagcgct tcgagcgcag
     2941 cgacctcagc cagagtcgtg acgttgtagg gctgcctcag cttgtccagt gcttccgcca
     3001 gccgcggggg gaacacgccg tatccgatcc gcagaccggc gaggccgtag atcttggaga
     3061 acgtccgcag gacggccaga ttgtcgtagt cgtcgagcca ggggatggtg tcctcgtggg
     3121 cgggatgggt gacgtactcc gtgtacgcct cgtcaagcac gaccagcgtc tcacgcggca
     3181 ccgactccac gaagcggcgc aaggcctgcg gccccacgta cgtcccggtg ggattgttgg
     3241 gctcgcagac gatgacaaga cgtgtctcgg ggccgatcgc agcgctcatc gcatccagat
     3301 cgtgcacgtg gtcacgcaaa ggcaccgcgt cgaacgatgc ttcgcgcttc aacgccacgg
     3361 tccggtagac cacgaacgac ggatcggcga acaccatatg ctcgccgggc tccaggagtg
     3421 cctcaccgag aagtatgagc aattcgcacg acccgttgcc gaaaacgagc ctctcgccgg
     3481 gcactccgtg ccgttcggct agggccgacc gcagctccgt gcacccgccc gatgggtagc
     3541 ggttcagcga agggagcgcc gtagtcagcg cctcgatcac ctcggggaag ggaccctccg
     3601 gacactcgtt cgccgccagc atcaccactc ggttcaggcc gtaccgctcg cgcagggatt
     3661 ccagcggggg accggccttg taggccgtca gcgcttccag agccttggtc aatctcatgt
     3721 tccagtccga tccggtccga gggcctacgc gcacatctct cagaggtcta ccaggcgtcg
     3781 cagtccctcg atctcactcg cttccaggtg gcgcgaagcg cccgagacca tggcggcgtc
     3841 cgacagcgga ccgaaaccta cccgatgcag ctccacgaca ggatggccaa cggcctccaa
     3901 catacgccgg atctgccggt tcctgccttc gtggatgcgc acgcccaact cactgcgggt
     3961 ctgcctcgag gacatcacca cgacccgcgc gggcgcggtg acgccatcct ctagatcgat
     4021 gcctgcagcc aggcggcgcg ccgtcacctc ggtcacccgt ccgacgacca cgacccggta
     4081 ttccttctcg acgccatagc tcgggtgcag cagccggtga gcaagcgctc cgtcgttggt
     4141 caagaggagc gctcccgtcg tgtccgcatc gagtcgccca acagggaaga tccgcacgct
     4201 cgcaggtacg cagtctacca ccgttcggcg tccttgcggg tcttttgccg tggagacgac
     4261 gcccttgggc ttgttgagca gccagtattc cagcgtctct ctgtgaatgg gaaccccgtc
     4321 cacgaggacg gcctgcagcg agggatccac cgacgaacct aactccgcgg tgcggccgtc
     4381 gacggtcact cggccttctc tgatcaggtc ctcgcacgag cgccgcgagc cgaggccggc
     4441 agaggccagc acacgctgca gccgctggat catcccggcg ccaccaccag acccagcctg
     4501 cgcttcagct cttccctctg gtcgtccccc acctcgaact cctcgatagg aggcagcgag
     4561 ccgatgtcgg cgagatcgaa cgccaccagg aaccgggtcg ttgtgccata caagacagca
     4621 gccccgggtg catcgcggcg gcctacttcg cgcacgaggt ctcgatcgag caacgtgtgg
     4681 agggccgagt cggagttgac accgcgtatc tcggcgatct gcgggcgaga gaccggttgc
     4741 aggtaggcca cgattgccaa cgtctcagtg gccgccgggg agagtcgcaa ctcctccccc
     4801 gcgtcgaaca ggcgagccag tgcaagttca cacgccgcgt tcgtccggaa gagccacccg
     4861 ccaccgaccc gggccagctc gaatcctttg tccccgccca ccgggtatcg ggattccaag
     4921 acttcgagcg ccgccgctac ggccgctttc tccatcggct cgtggtagga cgcagcttcc
     4981 ctcaggctat caagatcgag tgggcggccg gccgcgaaga ggagggcctc cagttccctc
     5041 gtcaggtggg cggccggttg gggtgcccgt tcggtggtcg gggccgccgg tccggatgag
     5101 tctgcaccca ggggatccgc gtcgatcatg cggttcctct cagagccacg cgctggatat
     5161 ccacatgctt gaccgtgatg tcgccgaagt gcgacgactg ggccaccttg acgtcccccc
     5221 gagagacgag accgagaaga gccaggagac tgaccgcctg caccatgggc tcctccgttc
     5281 cgaactcctc ttcgaacgag aagctaccgc gctcacgcaa caggcgccgg atggccgcga
     5341 tctgacggtt caagccgata cgcaacacgg cgacgtgcga agtgtcgggc agccgatgag
     5401 actccagcag cctgccgagg gcccgcgtca gggaggcggg ggcctgcgag ccccggatct
     5461 cctcgtcggg cgccagcgcg cgccgcggcg cgcgcgcagg ttcgcggacg aggcgccctc
     5521 tctgacgttc cctgacctcg gcaagcgatg cggccgccat cttgtagcgc cggtaggtga
     5581 gcagacgttc gaggagcacg tccttgctca actccgcgtc ggcgtcttcg atgcccacct
     5641 cttccccggg tagcaggacc cgggacttga gttcgagcag cgaactgatc aacaccagga
     5701 actctgtgag ggcttcccaa tcgaccgcgt ccgtcgcggc caactcctcg acgtaggcca
     5761 agatgacctc gacgatgggg acctcgaaaa tgtcaacttc ttcgcgcagt atcagggtga
     5821 agagaagatc gaacggacct tggtagacgt cgaggtcgag gtcgaggacc ctcatgagcg
     5881 gtggcgccgc accgcgcggg ggacgcgccg gctgaggagg tgctggcgtc gcgaatctaa
     5941 catagtcgag tccatatact acgtgacgcg ggccgagaag gcaaaagctg ccgcccggcc
     6001 ggccgggcgg gttccccacc gccggcccca gccgcggcgc ggctcgaacg ccgtcaaaag
     6061 ccgtaggccg gaagaagcag cctgctcaca agattgaacc ccccgccaag gaggctgaag
     6121 aacgggcgct gcaggacgaa gaagatcagc aggatgaaga gcatgccgta gcgatccagc
     6181 ccggcccagc gcgcgtaggt gctcggtgac atgaacccgg ctatcaccct cgacccatcg
     6241 agtggaggga tgggaaccag attgaagatg cccagcacca cgttcacctg aaagcccagg
     6301 aagagcgctc gaagaaggat cgacccgccg tacagagcct gcaccgacga tacggccgga
     6361 gcgagagggt tgaggatgcc ggccagaacc acggcgatca ggaagttcgt gaaaggcccc
     6421 gccagcccca ccagcatcat cccccgctgg tgatcgcgga aatacccggc attcaccggc
     6481 accggcttgg cccagccgaa gatgaagccg ccggcgaagt aggttatcgc gaacatcatc
     6541 gtgcccaagg gatcgaggtg cttgagtggg ttcgcggtaa ggcgcccatg agctttcgca
     6601 gtggggtcac cgaggcggta agcgaccgcg ccgtgcgcca cctcatgggc caccatcgac
     6661 agcagaagca cgggcatcaa cagtgcgaag ccgagtagat cggatatcaa ggcctatacc
     6721 taccttctgg aagtctgtca cccatcaaag ggtccgtcgc acgagggcgg ccgcggcggc
     6781 caattgctct tccctactct cgaggtcgcc ctcgatcctc agcctggtca actgcgcgag
     6841 cacccgcccc accccggatc cgctcggctg tccgagagcc atgatgtcgg cgccggtcac
     6901 gtccagcgag cgcttccgca ggacctcgag atactgccgc aggcgatcct gtcccggccc
     6961 tgatggagtc cgtccgagcg cgaagacgag cgactcgatc ggcaccttcc gcaggagtcg
     7021 gaagacgtcc caatctcccc tggcctcatc ggcaagttgc tcgaccaggt gcggtccgag
     7081 tatgacacta tctcgcacca cctgacggtc ggcgtgtctg agtctcagct tctccagcca
     7141 gatgaagagc tcttcgtgcg acatgttgcg ggtgatcgcc gccagacgca ggcgccacga
     7201 gaccacgtct gacgccaccc cgaactcttc gacgagcccg tccaagacct ggatgagccg
     7261 aacggtccgc tcgcccgtgg ccagtttggg gtgcacttgg cgcgcgacac cgagctcgaa
     7321 cagtctagca agggtccaat ccacatcctt ctctccgagc agggccacca gttcgtcccg
     7381 gagccgagcg ctggagagat cgcccaccag gcgcatctcg acacaggagc gcgccagcga
     7441 gcgggtctgt tcgtccatgc ggaacccgta acgattctcg tatctcacgg cgcggaagat
     7501 gcgggtggga tcctctatga agctgaggtt gtgaaggact cggatgactc cctcctgcaa
     7561 atccttcaac ccgccgaaga agtcgaggac ctcgccgaac tcctgcccct tcagggacac
     7621 ggccatggcg ttgatggaga agtcacgacg gaacaggtcc tgcctgatag atgcgtgctc
     7681 cacgctggga agggcggcgg ggtaatcgta gaattcggtg cgggtggtgg caacatccac
     7741 gtggaagggt tcggagctga cgagcggcga cgcttctccg tctcccacga cgtcgaccga
     7801 aacaagcacg acggccgtct ggaacttctg atgcgtgcgg accctgccac ccatcttcgc
     7861 cgcgagcctc ctcgcgaaat cgatgccgtc gccgtctacg gcgatatcga tgtccgcgtt
     7921 cggtttctcc aacaagaggt cgcgcacgaa tccccccacc agatagacgc ccgggaagtc
     7981 ctcagacagc gccgacaccg cacggaacaa cttgccgaac gccggaaggt cggtgagcga
     8041 gcggatggca cacaccgcgg gtgcgcccaa ctcccggtcg ggcacactgc cgacctgcag
     8101 cgcagccaac acgtcggtgc gcgtggcgat ccccactacc ccctccacct cgaccctgcc
     8161 gagctcacgc gcgcgctcgt aggcggcgtc ggtgaggatc ggtaggcggc ccacgttggt
     8221 ggaacccatg aggcgacgca cctcatccac cgtcgcggac tcaggggcga acacgacgtt
     8281 gcgcgtcatc acacccttga ccggcgcgtg gcccaagcca tgcctcaccg ccttgtccag
     8341 gtcgcggcgg gcgacgatgc ccaccacgaa ccccgcctct ttcaccgaga tgccggagtg
     8401 tccgtaccgc tgggcggtca gcagcgcctc gtcgaccggg gtctccgcgt cgatgaagcg
     8461 gaccggatga ctcatgatgt cgccggcagt gggaagagtc ggcaccgtct ccgccagtcg
     8521 acccaggagt ccgtccagga cgttctcagc cgacctgtcg cggaggaccg ccgaggccgc
     8581 ctgagcgtga ccgccgccgc ccaccgaagc caggagcccc gcgacatcca cgaccgcgga
     8641 gcgagacctc gcggtgacga agaccctgcc ttccatctcc acgacctgca acagcacgtc
     8701 ggagttgagc aggtccatca gcttgtgcgc gatcacgctg aggccgtcga catactcgcc
     8761 gtgcgacatc gccaccacat ggatctcttg tccccacacc tcgacgacgc gcacctcatc
     8821 caccatccgc agcaagatcg cccgctgctc cgcggtcagc gggttgtgca gatagtgctc
     8881 gatgagcgcc tgtgaggctc caaggcggag gcacagcgcc agcatctcgg cgtcgcgcac
     8941 cgtgctgcgc gggtacgtga gcgacccagt gtcttcgtgg atccccaggg cgaagatggt
     9001 cgcctccagc ggcgagatag gtacgtcccg ctcgcgcagg atgtgcacca tagaagtggc
     9061 ctgcgcaccg tccgtagaga gcacccagtt ctctccctcg acgaacgaag ggcgctctgg
     9121 agcctcttcc tcgtgatggt cgaagacgac gacctcgacg tcgggcctgc cgcagagcga
     9181 gccgagttca gcgagacgag cacagtccga cgtgtcgacc acgatgagtc gccgaacgga
     9241 tgcgaggtcg agtgaccgga accccacgat gggcaggtct tctccatgca gagcgacgaa
     9301 ctcccgcacg ttgcggttca gagagcccgc gaagacgata cgcgcctccg ggaacaggcg
     9361 ggaagccccc accgccgccg cgaacgcatc gaagtccacg ttctggtggg tggtgatgat
     9421 atcggcgact ttgatgtctg cagatgattg agacatgacc gccgtctagt atagccgggg
     9481 ctgacgcccg cagggccccg gctggtcgag cagcctcaga cggggtccgg cggcggaccc
     9541 gagggacccg aggagcccgt ggcgaccgac tcccctactg cgccaggaag tgcttgaggt
     9601 tgacggccat gacggccacc tgctggcggt cggcgtcgtt cgacaaggtc tcgtccgcgc
     9661 tgtccaggga gacgtccgcg aggatgccgg ccgccgccag tcgccgatag gcggacagag
     9721 tctcgtcgtc cacccgcacc tgcaggagag aagggtcgac ctcggtcgcc tccggcagtg
     9781 catcgcccgc cgcgcgggcg aacaccacca gaacatgccc gaccatcaca ggctggtcgg
     9841 gcatgaaggc cgccggaaca ccacccgtgc ctcgcatgac tgccctggcg gccacggcgg
     9901 cgatgtagtc ggcctcgtcc agcacttccg gatccccgac cacgtcagcg aaatcctgcg
     9961 cttcgtcggc cgccacgtcc caacccatcg tccgcaccac cactccggcg aactcccccc
    10021 tcgagacgct ctcggtcggt ccgaaccgcg agggtcctga gagcgacatg agtcctgtcg
    10081 tcacggcctc gtcgatcggg ccgccgagca cgtagtcggt cggagcggtc gcagtggacg
    10141 aggttgtctc gtcagacgtc cggtcgatga tccccgagtt ctgcaccagc atgtagacgg
    10201 cggccacggc ggccgcgaac gcagccacca cgacgaggtt cagcaggatc ctgcccgccg
    10261 tgagtcggcg gcggcgccaa tacgacggga cggtcccgtc ttcgtactcg ggttcgctat
    10321 cgcgtctcga acgaatcctc actagacccc ctcgggtatc tcgtccagaa tcaagcggcg
    10381 tggctccacc cgaccggtcc cgacatgaaa cgcggacgcc accagagctt caaccgcctc
    10441 ggcctcctgc ggcgtacgcg cgtggatcac ggccatcacg tccccccgat cgacctcagc
    10501 gccccccttc accttcagga caacacccac cgagtgttcg atggcggccc ctttcgattc
    10561 ccggcccgcg cccagtgcca aacacgcttc cgcgatagcc agggcggcga cctcctgcac
    10621 atgtcctccc tgagtcgcac gaacacgcag caccgccggg gcctgcggta gccccgtgcc
    10681 gtcgacagct gccgggtctg cgccctgcgc cgccagccaa cggcgcgcgg cctcccgagc
    10741 gctaccgtca tcgagatggg ccgccgcacg agcacgcgcg cgctccaacg tgtcgtcctt
    10801 ccgcgccatc acgaggagat ggcccgccaa gacgaggacg acctcccgca aatcggtcgg
    10861 tccgccaccg gagaggacct ccaacgcctc ccgtacctcc agagcgttgc caacggcgaa
    10921 gccgaggggc tcgtccatgt cgctcaggat cgcgcgaacg tcacgccccg catccctgcc
    10981 gaggtcgatc atggtccgcg cgactcggcg ggccttctcc atgtcgtgca ggaacgcacc
    11041 cccacccacc ttcacatcga gcacgatcgc cgtcgccccg gccgcgatct tcttgctcat
    11101 gacgctggag gcgatcagag actcctgttc cacggtggcg gtgacgtccc gcaatgcgta
    11161 caagagcttg tcggccggca ccagccccgg tgcctggccc acgatggcga caccgacctc
    11221 ggccacctgc cgccggaact cgtcggcggt cagctcggtt cgaaacccgg ggatcgactc
    11281 aagcttgtcg atcgtcccgc cggtgtgccc caacccccga ccgctcatct tcgcgaaggg
    11341 aacgccacac gaggcgacca acgggccgag aaccagcgac accttgtctc cgacgcctcc
    11401 ggtggagtgc ttgtcggcga cgatccctcc gacgtcggag agatccacca cctccccact
    11461 ccgcaccatg gcgtcggtaa gcacgaacgt ctcctgtcta gtcaagccgg caaagcagac
    11521 ggccatgagc caggccgcca cctggtagtc ggggatagcc ccggccacgt acgattcgac
    11581 cagccaccgt atctcgccgt ccgaatgttc cgcgccggat cgcttcgcgg cgatgaggtc
    11641 gaccgccctc atcccgcctc ctctcttgcg catccagcca acaggagggc gacgaccgcg
    11701 gcgaccttcg tcgccgcccc cgtcgaggat tccacgacct cccgatgcac ggcctcgccg
    11761 tccgctcccg tttcgcccgc cgcgttcgtc accacggtca acacggtgac cgtcatgcct
    11821 gcttcgcggg ccgcacgaac ctcctgcgcg gtcgacatcc ccacggcgtc cgctccaaga
    11881 cgtctcagta ccgccacttc gagcccggtc tcgtactgcg gcccggggag cgctgcgtag
    11941 gtcaccacag gcagcgactg cggcggcgcg cccttcaaca gctcgaggac ccgcggatcg
    12001 gtggcctcca actcctcagg ctcaccgtca gcaagaggca gttgaaagtc gaggatgcgt
    12061 tccgtcacgg ccacctgccc tggggccggc gacgctccga gcccgcccga tgcgttggtg
    12121 atcacgatgt tccgtacgcc ccaacatccc aacgtgcgca cggggagctg aaccatgccg
    12181 atgtcgaacc cttcgtagcc gtgcacccgg ccgcgcagga tcgcgacgcg gcagccctcc
    12241 agccgtccga cagccaggcg tccgctgtgc ccggggacgg agcccaccga ccagccgggg
    12301 atgtccgagt agggcgtctc gtccaattcc agcatgttgt cggccacggc atcgagcccg
    12361 gaacccagca cgatagccac gtcggggggc gcaccgtaac gctgttccag ccagtgggcc
    12421 gccgacaccg gcacttctcc accggccccc gaaggctgcg gcgccgaacg cacacacttc
    12481 cccccaccgc ggcgggcgac cggtatcgag gggtaagctc tccatccgta ctcagggcgc
    12541 agtcgctgca ctgggatgcc gcccaagacg gggtcgccgc cgatcagatg aaagtacgcc
    12601 gaggcccccg tatcgctcat cgagccgatg tacgcgctcg cgggagcgcc cgccggtcgc
    12661 gggtagtaga gcagcgggac gtactcacgg ctgtggtcgg tggacacggt cgtagggtcc
    12721 accccgtgat cggcggtgat cagcagtcga tcgctcggac gaagtttctc gagcagcgca
    12781 ggcagcctgc gatccaccgc ggccaacccc tcggcgaagc cttcgatgtc gttgcggtgg
    12841 ccccacacca tgtcgaaatc caccagattg gtgaagatga ggccgtcgtc accgtcgtcg
    12901 aggagctcga acagccgctc catgttctcg tcgttcgaac ccaccttgat ctgccggccc
    12961 actccgcgac cggcgaagat ctggtggatc ttgccgacac cggtgaccgg cacgccggca
    13021 gcggtcagta ggtcgagata cgtgtcactc ggcggctcca gggagaagtc cttccggtcc
    13081 ttggtgcgca cgtacgcgcc cgccggtccc gcgaagggcc gtgcgatgac ccgaccgacc
    13141 gcgtgcggcc cggtcaagag gtcgcgggct atctgacagt atcggtagag ctcctcgagc
    13201 ggaatcacgt cgacgtgggc ggcgatctgg aagacgctgt cggcagatgt gtagacgatc
    13261 ggagatcctg acgccacgtg gagtccaccg agttcggcga tgatctcggt gcctgaggcg
    13321 gccacgttcc cgagcgtctt ccggcctatg gccttctcga aaggtccgag cacttcctcc
    13381 gggaagccga agggataggt ggggaagggc gaagtggtca cgacccccat gtgctcccaa
    13441 tgtcctgccg tcgtgtcctt gcccgccgac ttctctgcga ggaccccggg gagcgctcgg
    13501 ggagcggccg ccggcgccac tcctgtgaga ggcagtgcgt tgcccagacc gagagcagcg
    13561 aggttgggaa cacgcagcgg gaccagcttc gcgaggttgc ccagggtgtt ggagcccgcg
    13621 tccccgtatg atgcagcatc gggcagggcg ccggcgccga cgccatccag gatcatgacg
    13681 atgaaccgtg ccatcactcc gtcctcgccg agcgggcgcg cggatgagcg tcatcgtaga
    13741 cccgccgcag gtgcgccgtg ctcaggtgtg tgtagatctg cgtggtggct agatccgcgt
    13801 ggccgagcat ctcttgcacc gcccgcagat ccgctccgcc ttccacaagg tgcgtcgcaa
    13861 aggagtgccg aagggtgtgg gcggagacgt cgctgctgag gccggccagc gccgcgtact
    13921 tcttcacgat gaggtggagg ccctgtcgcg acagaccctt gccccgagtg ttcaagaaga
    13981 gctggggagg tttcaggccg ccgggcagcc ccagcagggg ccgcccgcgg gccacgtaga
    14041 tgcccaccgc ttcgactgcc ttccgaccca gcggcacgac acgctccttg gagcccttcc
    14101 ccatcgtccg cacgaagccc acctccagat cgaggtcctg gagtctcagc cccactcctt
    14161 cggacgcgcg caggcccgcg ccgtatagga cctccagaag ggcacggtca cggaggccgg
    14221 ccggcgtagc cggcggccgg gtgagcagcc ggtccacctg ctccaccgtc aggggcttgg
    14281 gtaatcccgg cgacatccgc ggggacgcga ggcctgtggc cgggtccgcg gcaagggcgc
    14341 ccacgcggac caggtggctg tagagagaac ggatggccgc ggtcttgcgg gcccgcgtgg
    14401 acggcttcca gtccccggcc gcgaggaagc cgcgaagatc gtcggtgagc gcttcctcga
    14461 cgccgatccc agctcccgcg aggtgtgccg tcagttgcgt gatgtcccga cgatacgagg
    14521 ctaccgtgtg atccgccagt ccatgctcca gccggaggca ggtgaggtag tccgccaccg
    14581 ccggaggcag gggcgccgcg gccgagggcg cccccacagg ccggtccgaa ggccccagcc
    14641 cagtatccgc cggcgtcggt ctcaagtcga cctcatccac accacggcgc acagcccggc
    14701 ctcaagagaa ctctatcggc aggaggctct ccaacggaaa gtaccggtta cctgtcgctt
    14761 tcgccacttg ggagttggtc accttgccct ccatgacgtt gatccctcga gcgagagccc
    14821 tgtcgctgcg ggccgcggcc accaacccct gctccgccac cttcatgatg tagggcaacg
    14881 tggcgttcgt cagcgccagg gtcgacgtga tcggcacggc cccggccatg ttccccacgc
    14941 agtagtgcac cacctcgtcc accacgaagg tcgggctgct gtgcgttgtc atgtgggacg
    15001 tggcgatgca tccaccttgg tcgacgctga tgtcgacgac cactgaaccg gcctgcattg
    15061 ccttcaccag gtcctccgag accaactgcg gggtccgcgc tcccgggacc aacaccgcgc
    15121 cgatgaccac gtcggcgcgc tccacctgct cttcgatcgt caactggttg ctcatcagcg
    15181 cttccacgcc cggaaccatg cgctcgacct cggcgagccg ctcgaccttc acgtcgagta
    15241 tggtgacccg cgcccgcatg cccgaagcca ccagagctgc gttcgatccg gcgatgcccg
    15301 ctcccaagat gaccaccttc gccgccgcga cccccgtgac ccctgccaac agctttccct
    15361 tgccgccgtt catccgctcg aggaagtagg cggccgcctg cggcgccaag cgtcccgcta
    15421 cctcactcat aggggccaga agaggcaggt gtccgctgtc ctgccccaca gtctcgtatg
    15481 caaccgagac acaaccactg tcgaccagac ctcgagtgag ctcagcgttc ggcgccagat
    15541 gaaggaaagt gaacaggacg tgacccgagt ggaggcgcag acactcctgc tcgagcggct
    15601 ccttgacttt cagtatcagc tcggcgccag agaagactgc ctcggcactc tccgctatct
    15661 gcgcccccga cgccacgtag gcttcgtcgc cgaacgccga accctcaccc gcgagcgtct
    15721 cgacaagaac ggtgtgccct cgggaagtca actctcgcgc cccagcgggc gtaagcgcca
    15781 cgcgatactc gtccgccttc aactccttca gaacgcctac ccgcatgaca tgcgcctcct
    15841 atgctccctg gtcttcatcc ctcgcaagat gccgttgcag caagagaagc gtcgcgagcg
    15901 tcttcgcgtc cgtcactgcg gggacaccgc ggacgagatc ctcaagtcgc acccacacca
    15961 cctcgatgtc ctcttccacg tccagatcct ggaccgtgga gacgagtccc cgtgccaaga
    16021 aggcatgcag ctcttcgtgc aggaacccgg gcgaagagaa gaacgagccc agagacaccc
    16081 actcatgggc tacaagcccc gcctcctccc ggagctccct gcgtgcggcc gcccccgggt
    16141 cctcgcctgg ttcaagccct cccgcgggga actcgagcag ttcccggccg acggcatgac
    16201 ggaactgacg caccaacacg acccgttccc tctcatcgag ggccgccatg acgaccgcac
    16261 cggggtgttc caccacctct cgagtcacag tcccggtcgc aacgcgcacc tcatcgaccc
    16321 taaggctgac gactgcgccc gagtacacag tccggctgct gaggatctcg ggcggcgcct
    16381 cggcctgcgc ctggtcctgc atcccagacg agtccaccca tcctgccgcc gtcaccccgt
    16441 cgggtctacc ctcacccaac tcagacgact ccttcggagc cgcttcttcc gccgccggca
    16501 ggctcggcca tgacgatgac gttccgcacc agttcaccca gcaggacgag ctgagcgacg
    16561 tccatcgact cctccgcagt gtgcacgcgg ctcatcccgc aatcgaagtt gacagtgggg
    16621 gtgccgcgac cgttgaagac gttcgcgtcc gaacctcccc cgctcgcgtg cgtgcgcgcc
    16681 ggcagtccga gcgcttcgat cgcccggcgg gccaagccca cgactgccga agactcatcg
    16741 agccggaacg cacggtactc cgtcaccacg tcgaggtcga ggtccgtccc cactctggca
    16801 gcgcccaact gcagggcatc gatcatgcgt gacgatgtgg ccgcgagcat ctcgtcgtcg
    16861 agacttcggc actcggccac cactcggcac gactcaggga cgatgttctg ggccaccccg
    16921 ccctcgatca tgcccacgtt cgctgtggtc tccgggccga gtcgacccaa ctccatcagc
    16981 gagatggcgt gggcggcggc ttggatcgca ctcctgccgt tctccggctc caaaccggcg
    17041 tgggcggcct gaccgcggaa tgtcgcgcgg atcgtctgct ggctcggcgc ccgcaccact
    17101 attccgccga ccggacccga tgagtcgaag acagccgcta tcggcgcggc cggcagatcg
    17161 ggggcgagga aacgtgagcc cagcacaccg acctcttctg ccacgctgaa gatcaactca
    17221 aaggtgggga aggcatcgcc accgaccact aatccttccg tcacggtcac gagcgcggcg
    17281 acggcggcct tgttgtcggc gccaaggatg gtcccgctcg agctgcggat cacgccgtcg
    17341 ttcagcgccg gtcggatggg gccggacggc tccacggtgt cgaggtgcgc ggcaaggcac
    17401 acggacggcg acgcaccgcg gccccgcacg acacaccgca aattccccgt gtcgcctccc
    17461 acccgctggg cggcgtcgtc ttcgacaacc tccagcccca ggccgcgtag cctggcagta
    17521 acaaggtccg ccgccgccct ctcccgccgt gacggactgg aaatcgaggc gagctccatg
    17581 aagagtgcta ccagaccgcc cgcctcgttc gaaggtgcgt ccgtcactcc gagaccccgt
    17641 ctgctccctt gtccacgccg cacgaacact cgatggccgc gccgacgaaa tcccggaaca
    17701 gcggctgcgg cctcgtcggc ctgctcttga actcggggtg gaactgcgag gccacgaacc
    17761 aggggtggtc ttcgagttcg atgatctcca ccaatcggtc gtcggacgtg acgccgctga
    17821 tcacaagccc agcctcgacg agcctggcgc gcaggccgtt gttcacctca tacctatgac
    17881 gatgccgttc gtacacgacc tcctcaccgt acgcggcgaa ggccttcgta cccgggatca
    17941 ccttcacggg cgatgcgccg aggcgcatgg tgccgcccat atcctcgata tccttctgct
    18001 ccggaagcag atcaatcacg gggaacggcg tctccgggtc gaactcgaag gagttggcgt
    18061 cgttcatgcc ggcgacgtgc cgcgcgaact cagccaccgc cacctgcatc ccgaggcaga
    18121 tgcccaggaa gggcaccttc ttctcgcgag cccattcgat cgccaggatc ttgccttcga
    18181 tgccgcgcac cccgaaccca cccggcacca ggatgccgtc ggcggtctcg agttgggagc
    18241 gtatctcctc gggccgcagg ccctcggcgt ccacccatac cacctccacg tcgcggccgt
    18301 ggtgaagagc cgcatgcctc aaagcttcca caacactcaa gtaggcgtcc tgcagttgca
    18361 cgtacttccc cacgagcgca atgcgcacga tcccggagcg ggctcggata cgcgccacca
    18421 gcgcttccca ctcgctcaga tccaccggcc cgcactccag gccaagcttg gccacgacca
    18481 gatcgtccag accctcgtct cgcaggttga gcggcacgaa gtagatgttc gccgcgtcga
    18541 tggcggagat cacggcgcga ggttcgacgt ccgtgaagag cgcgatcttg tccttgagct
    18601 cgcgactcag tgggcgatcg gacctacaca cgatgatgtc aggctggata ccgatctcac
    18661 ggagttcgtt cacgctgtgc tgcgtcggct tcgtcttcaa ctccccggcc acgccgatat
    18721 acggaatcaa cgtgacgtgg atgtagagaa cgttctgccg accgatatcg ttgcggaact
    18781 gacggatggc ctcgaggaag gggaggctct cgatatcgcc gacggtgcca ccgatctccg
    18841 tgatcacgac atctaccggt agattccgcg agactctcga gatccgttcc ttgatctcgt
    18901 tcgttacgtg cgggatcacc tggacggtgc cgccgagata gtccccccgc cgctccttgc
    18961 ggatgacgtt gtagtagacc gacccggatg tgatgttcga ttccctggtg agattctcgt
    19021 ccacgaagcg ctcgtagtgc cccaagtcga gatcggtctc agcgccgtca tccgtgacga
    19081 acacctcgcc gtgctggtag gggctcatgg taccggggtc gacattgaga tacgggtcga
    19141 acttctggat caccaccctc aacccgcggc tcttcagcag ccggcccaat gaagcggccg
    19201 tgatgccctt gcccagggat gagaccaccc cgcccgtcac gaacacatgt ttcgccatgt
    19261 acctttcctc gccggtcgct gtcgaatcgg gaaggggcgg aacgcggggt cccgcgcccc
    19321 atctagtcta tcaggcctac ctccgccctg tgtttcaaag accgtgcgag cgccaatgcc
    19381 ccttggtctc cgggcgcccc gcccagcatc cgggccagtt cctccaggct cccttggccg
    19441 tcgagaacct ggaggctggt cacgctgcgg cctgccgcga cctccttgcg gatgagatag
    19501 tggcggtcgg cgaaggcggc tacttgggcc agatgcgtga ccacgatcac ctgacagttc
    19561 cgcgatgtct ctctcagctt ggcgcccact gctgtggctg tgagcccgcc tacgccggcg
    19621 tcgatctcat cgaacaccac ggtctccgcc gtctcgcatc ctctcagagc ggtcttgatc
    19681 gccagcagca cgcgcgacaa ctcgcctccc gaagcagtgc gcgcgaggct gcgcgcctcc
    19741 actccaacgt tcgccgcaag gaggaactcc accgagtccg caccgtccgc gtcgagagcg
    19801 tcccatccct gccgtgactc cacggagaca ggcgctgtgc cctcagccat gccgagatcc
    19861 acgaggtgtg gccgcacggc tgcctgcagc cgtgcggccg cttcgacccg acgcttcgac
    19921 agttcggccg ccaactccaa acagcgacca cgccgctccg caacgtgccg cgtcatctcc
    19981 gaaaggtcct gctccgacgt ctccagcgac cgcagccgtt cagaagcctc tgccagaaac
    20041 gcgatcgcag cctcggtggt cccttcgtat ttcttggcca tgtcactgta gacacgcaat
    20101 cgttcgtcca ccgcgttgag tcgagccggg tccggctcga gcgaacccat gagtgtatgc
    20161 agcgtccggg ccacgtcgtc gatcagcccg tcggcctcgg agagcgatgc gagtgaatcg
    20221 tccaggcggg ggtcgagccc gaggaacggc gtcaaccctg atcgcgccgc cgataccaac
    20281 cctccaacat ccgcgccgac gtcgtcggag cgcagacagc cggccgccgc cgcacacgcc
    20341 cgtgccatct cttcgatacg cgacagccgg cgctgctcca cttccagttc gcgctcctcg
    20401 cacacctcca gagcggccgc ctcaagctcc tgcacttgga accgcagcaa ctccgactct
    20461 cgccggcgct cagccactcc ccggcgtaag gcgtcgagcc gtgcttcacc gctccgcgcc
    20521 tccacccaac acgcccggta ctcctggcga tcacgcgtct ggtccgagcc ggcgaaggca
    20581 tcgaggacct gcagttggtg cgagggcttc agaagccgac gatgctcgtg ctgacccgag
    20641 aacgagatca gatcacccag ggcttcggag agcaccccaa gaggcacggc gacaccgttc
    20701 aggaagcaac ggttcttccc cacacgggag agtctgcggg tgaccacgag ttccccagcc
    20761 tcgaggtcga gcaggtccct ggtgtctgcg ggcacctccg caagttgcga ttccgagagc
    20821 tcaagaacac cctgaatgag agcctcctgg ctcagttcgc tcacgagccc gtcgcccgcg
    20881 cgctccccaa ggaggagccc aagggcttga gtcaagaggg tcttgccggc gcccgtttcg
    20941 ccgctcagga ccgtgagtcc tgagccgaac tccaacgaag cgtcctcgat aagaacgtaa
    21001 tccttgatgc tgagggaggt aagcactcgg tccccgggac tagacgaatc gttcagcgaa
    21061 gtgctggtag aagtccgttc cgggaagcag cgcaagcgcc gccagggccg ggatcgtgcc
    21121 cacctcgagg ttcgccaacg gggccaactc ggcgacgtgc tccccgtctg agagcacctc
    21181 gactgcggag tgagtcgact cgttggtcag ggtcagcaca tcacccgcag cggcgacgac
    21241 cgaacgcacg ccgagtgcat gcggagcgac gtatgacacc acgaacccct ccacacccac
    21301 gccaactaca ggccccccca ccgagaggtt gtaggcagtg gaacccgctg gagtcgccac
    21361 gacgagcgcg tcgcagcgca cgtcgaacag gcggttcccg ttgagcgcgt acgagatgcg
    21421 gcaaacgccg ccctccctcc cgcgccctac cacgaagtcg ttgaccgcgc gcagtgtgac
    21481 cccgtcgcac acagtggaca gacccaagag tggctgcgtg acgaactctc ccgccaggag
    21541 gcggggcagg tcctccacga tccggtcccg tccgaccgtc gccaggtagc ccacccgacc
    21601 cagattcacc ccagccaccg ggacccccag gtcgcgcgtc atccggagcg ctcgcagcat
    21661 ggtgccgtct cctccgaaca ccaggcac
//
LOCUS       DASRMJ010000324         5137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_70578, whole genome shotgun sequence.
ACCESSION   DASRMJ010000324 DASRMJ010000000
VERSION     DASRMJ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70578"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFD74_08965"
     CDS             <1..895
                     /locus_tag="VFD74_08965"
                     /inference="COORDINATES: protein motif:HMM:NF024235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZK49712.1"
                     /translation="PQVVSPLTVMRDGSEREFAMPTECPSCSTPVVRIEGAVAVRCPN
                     PDCPAKAVEVLKHYVSRGAMDIEGVGDKIGRRFFELGLVRDPADLYLLSVDDLLPLEG
                     FQEKAARNVLDAIEASKGRSLSRVLFALGVPHVGAQTAEILVARFRSMAALRDASADD
                     IAAVEGVGPIIAESVHAYFRDPRIIDLIGRLAGAGVRMEEAGPVSSAVQGPFSGRTFV
                     LTGTLPSLTREEAGGLIEARGGRVVGSVSAKTDYVVVGESPGSKLDKARSLGVTELDE
                     ASLKELADLSGSGRAARALSP"
     gene            complement(942..1970)
                     /locus_tag="VFD74_08970"
     CDS             complement(942..1970)
                     /locus_tag="VFD74_08970"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HZK49713.1"
                     /translation="MRRSSLIGAAVLFAFATIALLLSAVPATRAAAAGNVEAAARAHE
                     EAVVQLLIIERRVADAEAALVEADDRLQQSEAAVTEWTRSAESAAADLAEAKRRYAEH
                     MVEAYKTGGAGWLELLFGSDNLSEFIGRTLLIGRILTQDAALADKVEETRAHAQETAS
                     RADSAAREQATQVSGLRATRDTLEKAKREQTALVDSLGDRLEAARAAAQAAAAKMAEV
                     NETVRNDGGSGTTTTTTDPPGGGTDPPVGGTDPPSGQGRQLTVKSYAYALRGTTATGV
                     PVARGVIAVDPRVIPLGTRLFVPGYGEGIAADTGGDIKGNTIDVWMSSLQAATDWGTK
                     IITITVYD"
     gene            complement(2086..2913)
                     /locus_tag="VFD74_08975"
     CDS             complement(2086..2913)
                     /locus_tag="VFD74_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZK49714.1"
                     /translation="MADVSGIQRAYELRLRRLLDELTAEVGADEEQQRQLVMLHGLAT
                     AVVQAYGAHQTLRAERLDSSTSVMYAADAGGGGALNVPLGSLVENGSLSSWQARFLTA
                     SLGLKRTILITGPTGSGRSTLLNSLVQLITVDQRIVAIEGDEALPALRDRSFTVHIPA
                     QTGTPSLADALEKGAAMQPTWVLVECLGGTDGPSFLRTLYAGFSGLATVDSPDPELVL
                     SEWTTHNAQAALHLRKIVPLVVHMDRDPGGRPRALKVWEAGVNEKTGRLVLNEKRPD"
     gene            3113..3286
                     /locus_tag="VFD74_08980"
     CDS             3113..3286
                     /locus_tag="VFD74_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49715.1"
                     /translation="MEKRDIQHEVEQVATDGRLDCHTARVLAEKLGVGYADVGAAADE
                     AGVKIHSCELGCF"
     regulatory      3336..3464
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3591..4556
                     /locus_tag="VFD74_08985"
     CDS             3591..4556
                     /locus_tag="VFD74_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZK49716.1"
                     /translation="MRSKGGSEGRRSKAAVMKGGLPYWFAAVVLSVLVLAALAFAGCG
                     GGSTTTTAADTTTTAAESTTTSEATTTTVKPSDIILASTTSTQDSGLFDVLLPAFAKA
                     YPQYTVQVIAVGSGEAIKLGEQKDADVLLVHSPAAEKDFVANGFGTERRLVMYNDFVL
                     VGPESDPAGVKGMTDAASAFAKVAAAKARFISRGDKSGTNTKELGIWKAAAIEPAGDW
                     YESIGQGMGEALRISSERQGYTLSDRATFLNLKDTLELVVLVEGDKAMFNQYGVIPVT
                     DATNAQGAQDFADWITSAEGQAVIKGYGVEKFGQPLFVPNAGSEG"
     gene            4629..>5137
                     /locus_tag="VFD74_08990"
     CDS             4629..>5137
                     /locus_tag="VFD74_08990"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49717.1"
                     /translation="MQLFWDAIVEAFRLLGSLNSYVFQVIGLSLEVSGSAVLIGLVIG
                     IPLGLWLGLSRFPGRSLLVALVNTGMGLPPVVVGLFVLMMLSRRGPLGFLSLLYSPPA
                     MVVAQVILATPYIAAITMSAVGSLPREARLQALGLGASRAQSILLVLREARVGVMTAV
                     IAGFGAVIS"
BASE COUNT          781 a         1653 c         1797 g          906 t
ORIGIN      
        1 ccctcaagtc gtgtcgccgc tcaccgttat gcgcgacggc agcgagcgtg agttcgccat
       61 gcccactgag tgcccttcgt gttccactcc cgtcgtgcgg atcgagggcg cggtcgccgt
      121 acgctgcccc aatccggact gccccgccaa ggcggtcgag gtgctcaagc attacgtcag
      181 tcgtggggcg atggacatcg agggcgtcgg cgacaagatc ggccgccgct tcttcgagct
      241 cggtctggtt cgtgatcccg cagacctcta tctgctgagc gtggacgacc ttcttcccct
      301 cgaaggcttc caggagaagg ctgcgcgcaa cgtgctcgac gcgatcgagg cctccaaggg
      361 gcggtccctc tcaagggtgc tgtttgctct gggcgtcccg cacgttggtg cacagaccgc
      421 ggagatcctc gtggctcggt tccgcagcat ggccgccctg cgcgacgcct ccgcggacga
      481 catagccgcg gtcgaagggg tcggtccgat cattgccgag agcgttcacg cctacttccg
      541 ggatcctcgg atcatcgatc tcatcggacg cctggccggt gccggggtgc gtatggagga
      601 ggcgggccct gtctcgtcgg cagtccaggg gcccttcagc ggcaggacgt tcgtgctcac
      661 cgggacccta ccctccttga cgcgggagga ggccggcgga ctcatcgagg cacgtggcgg
      721 ccgtgtcgtg gggtccgtca gcgccaagac ggactacgtg gtggtggggg agtctccggg
      781 ctcgaaactg gacaaggcac ggtctctggg cgtgacggag ttggacgagg cgtcactcaa
      841 ggaactggcc gacctcagcg gttcaggtag ggcggctcga gcgctgtcac cttgacggct
      901 cgccgggcgc ggcgctgccg gccccggctt cgcggagtct ctcagtcgta gaccgtgatg
      961 gtgatgatct tggtgcccca gtcggtcgcg gcctgcaggc tcgacatcca cacgtcgata
     1021 gtgttgccct tgatgtcgcc gcccgtgtcg gcggcgatgc cctcgccgta gcccggaacg
     1081 aagagccgcg tgcccaacgg gatcacccgg gggtcgacgg cgatcacgcc ccgtgccacc
     1141 gggaccccgg tggccgtggt gcctcgaaga gcgtaggcgt acgacttgac cgtcagttgg
     1201 cgtccctgac ccgaaggagg atccgtcccg ccgacgggag ggtccgttcc gccgccagga
     1261 gggtccgtgg tcgtcgtggt agttccggac ccgccgtcat tgcgcacggt ctcgttcacc
     1321 tcggccatct tcgcggcggc cgcctgcgct gccgcccgcg ccgcttcgag gcgatccccg
     1381 agggaatcga cgagggcggt ctgctccctc ttggccttct ccagagtgtc gcgggtcgcg
     1441 cgcaacccgc tcacctgcgt ggcctgctcg cgcgccgcgg aatccgcgcg cgaagccgtt
     1501 tcctgcgcgt gcgcccgggt ctcctccacc ttgtcggcga gggccgcatc ttgggtgagt
     1561 atccggccga tcagcagcgt gcgacctatg aactcggaga ggttgtcact accgaagagt
     1621 agctccagcc agcccgcgcc gccggtcttg taggcctcca ccatgtgttc cgcgtagcgt
     1681 cgcttggcct cggccaggtc ggcggcggcg ctctcggccg atcgggtcca ctccgtcacc
     1741 gcggcctcgg actgctgcag gcgatcatcg gcttcaacga gagcggcctc ggcgtcggcg
     1801 acgcggcgct cgattatgag cagttggacg acggcctctt cgtgggctct cgccgccgct
     1861 tcgacatttc cggccgccgc ggccctcgtc gcggggacgg ccgaaagcag cagcgcaatg
     1921 gtggcgaagg cgaagaggac tgcggcaccg atgagtgatg agcgtctcat ggggagcgta
     1981 tagtaccagc ctggcgtgat gcggacaaag ctccggccgg ggtcccggct tcatggcggc
     2041 gtgccgccgg gccggccgac cggccggccc ggcggcgcag gccgatcagt cggggcgttt
     2101 ctcgttgagc acgagtctgc ccgtcttctc gttgacgccg gcttcccaca ccttcagagc
     2161 gcgtgggcgg ccgcccggat cgcggtccat gtgtacgacg agtggcacga tcttcctgag
     2221 gtggagtgcg gcctgcgcgt tgtgcgtcgt ccattccgat agcacgagtt cggggtcggg
     2281 tgaatcgacc gtggccagac ccgagaagcc ggcgtacaac gtccttagga aggaggggcc
     2341 gtcggtgccg cccaaacatt ccaccaggac ccaggtgggc tgcatggccg cacccttctc
     2401 gagcgcgtcg gcgagcgagg gagtgccggt ctgggcgggg atgtgcaccg tgaacgagcg
     2461 gtcgcgcagg gccggcagcg cctcgtcgcc ctcgatggcc acgatcctct ggtccacggt
     2521 gatcaactgg accagagagt tgagcagcgt tgagcggccg ctccctgtag gtcccgtgat
     2581 caggatagtc cgcttgaggc ccagcgaggc ggtcaagaag cgggcctgcc aagagctgag
     2641 ggacccgttc tccaccagac tccccagcgg gacgttgagc gcgcctcctc cccccgcgtc
     2701 cgccgcgtac atcactgagg tgctcgagtc caggcgttcc gccctgaggg tctgatgagc
     2761 tccgtatgcc tggacgaccg ctgtggcgag gccgtgcagc atgacgagtt ggcgttgttg
     2821 ctcctcgtca gccccgacct ctgccgtaag ctcgtccaac agcctacgca gtcggagttc
     2881 gtacgccctc tgtatgccgc tgacgtcggc catgtcgctc ccgtcggatc gggtgcggcg
     2941 ctcgcggatg ccacggtcag cactcttgga agcgagcggc gcctctctag ttcaaactac
     3001 tatacaggcc gcagtcccgc ccgtgcacac agcggggatg cgcacagcgg gcgtccgacc
     3061 atcggggagc ggcgttcgca tcgccggtcc caccgtctgc taatatcgga gcatggagaa
     3121 gagagacata cagcacgagg tcgagcaggt ggccaccgac ggccgcctcg actgtcacac
     3181 cgcccgcgtc ctcgccgaga agctgggtgt aggctacgcg gatgtcggtg cggcagccga
     3241 tgaggcgggc gtcaagatcc attcgtgtga gttgggctgc ttctaggcga ccccgccgag
     3301 agtggccctc acagacatag gcgcgtgagc gcgccgcact agtgagtctc cgagcccaga
     3361 cagcctaagg tcgccgatcc ggcggctatg gccctcgggc cggtagggta cagctggaaa
     3421 cggccgtgtc tcccatagcg gaaaggagac ctcctcgatg gtttgagggg acccgcgcgc
     3481 aagcgcgggt tttttgttgc agggttcctt tgcgggctgg gccggatcga cgtcgcgtcg
     3541 cggcggagag actcgagggt cgatgtctct acagatcgaa ggaggagttc atgagaagca
     3601 aagggggttc ggaaggtcgg cggtcgaagg cggcggtcat gaagggcggc ctgccgtatt
     3661 ggttcgctgc ggtcgtcttg agcgtgctgg tgctcgcggc gcttgcgttc gccgggtgcg
     3721 ggggcggtag taccacgacg acggcagccg acaccaccac cacggcggcg gagtcgacga
     3781 cgacgtccga agcgaccacg acgaccgtga agccgagcga tatcatcctc gcgtcgacca
     3841 cgtccacgca ggactccggc ttgttcgacg tgctcctgcc ggcgttcgcg aaagcctacc
     3901 cgcaatacac cgtgcaggtg atcgccgtgg ggtcgggcga agcgatcaag ttgggcgagc
     3961 agaaggatgc cgacgtactg cttgttcact cccccgccgc ggagaaggat ttcgtggcga
     4021 acggcttcgg gacggaacgg cggctggtca tgtacaacga tttcgtgttg gtgggccccg
     4081 agagcgatcc ggccggcgtg aagggtatga cggacgctgc cagcgccttc gcgaaggtag
     4141 cggccgcgaa ggcccgcttc atctcgcggg gagacaagtc cggcaccaac acgaaggagt
     4201 tggggatctg gaaggccgcg gcgatcgagc ccgcgggcga ctggtatgag tccatcgggc
     4261 aggggatggg tgaggcgctg cgcatctcat ccgagcgcca gggctacacg ctgagcgacc
     4321 gggcgacgtt cctcaacttg aaagacacgc tggagctggt cgtgctggtc gagggtgaca
     4381 aggcgatgtt caaccagtac ggtgtcatcc ccgtgaccga cgccaccaac gcccagggcg
     4441 cccaggactt cgccgactgg atcacctcgg ctgaaggcca ggccgtcatc aaaggatacg
     4501 gcgtagagaa gttcggacag cccctgttcg tgcccaacgc cggcagcgag ggctagtcga
     4561 gacgctgaag tgaaccccgc cggcgccgcc ttcgcgggcg gcgccggcgg gctggatcga
     4621 ggagatcgat gcaactcttc tgggacgcga tagtcgaagc cttccgcctc ctcggcagcc
     4681 tgaacagcta cgtgttccag gtcatcggcc tgtccttgga ggtgtcgggc agcgcggtcc
     4741 tcatcggact ggtgatcggc atccccttgg ggctctggtt gggtctgagt cgcttccccg
     4801 ggcgatcgtt gctcgtggca ctggtcaaca cagggatggg tctgcctccg gtggtggtgg
     4861 gcctcttcgt cctcatgatg ctgtcccggc gaggcccgct gggctttctc agccttctgt
     4921 actcccctcc agcgatggtc gtcgctcagg tgatcctggc cacgccctac atcgccgcga
     4981 tcacgatgtc ggcggtaggt tccctgccgc gcgaagcccg gctccaggcg ctcggactcg
     5041 gcgcctcgcg ggcgcagtcg atcctgctcg ttctgaggga ggctcgcgtg ggtgtgatga
     5101 cggcggtcat cgcgggcttc ggcgcggtga tcagcga
//
LOCUS       DASRMJ010000325         3825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_356146, whole genome shotgun sequence.
ACCESSION   DASRMJ010000325 DASRMJ010000000
VERSION     DASRMJ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356146"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1236)
                     /gene="mtbB"
                     /locus_tag="VFD74_08995"
                     /pseudo
     CDS             complement(1..1236)
                     /gene="mtbB"
                     /locus_tag="VFD74_08995"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            complement(1324..2088)
                     /locus_tag="VFD74_09000"
     CDS             complement(1324..2088)
                     /locus_tag="VFD74_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49718.1"
                     /translation="MEGLGAESGGGRAEAGGHPPLLEVTDIHVFYGNIHALKGVSLYI
                     ERGEIVTLIGANGAGKSTTLNTISGLLRPRSGGIVYDGADLTKVPAYETVKLGLSQSP
                     EGRKIFPRMSVKENLSMGAFSRPGQDLRADFDRVYGLFPLLHERRTQAGGTLSGGEQQ
                     MLAIGRALMASPKLLMLDEPSMGLAPMLVEKIFEIIREINGQGTTILLVEQNANVALE
                     ISHRGYVLETGAIVLSDDARALLSNPQVQEAYLGGA"
     gene            complement(2118..2912)
                     /locus_tag="VFD74_09005"
     CDS             complement(2118..2912)
                     /locus_tag="VFD74_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49719.1"
                     /translation="MSGAPDAAVSAGPILEIEALTKSFGGLVAVNSIGLTVHRDEIVG
                     LIGPNGAGKTTLFNVITGVYTPTSGRVLFDGREIGGHRPSKITSMGIARTFQNIRLFG
                     EMTALENVMVGRHCRTKTGLMGVVARTPGSLREERGVRSRSVELLEYVGLKGSVSELA
                     RNLPYGSQRRLEIARALATDPKLLFLDEPAAGMNPQESQELVGLVRRIRDSGLTVVLI
                     EHDMRVVMGICDTVFVLDHGEKISEGTPAVVRADPRVVEAYLGKSA"
     gene            complement(2896..>3825)
                     /locus_tag="VFD74_09010"
     CDS             complement(2896..>3825)
                     /locus_tag="VFD74_09010"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZK49720.1"
                     /translation="VLASGLNIVVGFTGLLDLGYVAFYAIGGYYTAIIMVQLVRNTWG
                     IELDSVWWLVWLNLIMGGVVAAVAGAILGYPTLRLRGDYLAIMTLGFHEIIRIVANNW
                     VGLTRGPSGISGIPKPSVFGFDFNTAFRFYYLALALAILSLFVISRMVRSRIGRAWTA
                     IREDEVAAEAMGIHTSRYKLLAYAAGGFFAGVMGAFLGHYLNFIHPSNFRLMESIMVL
                     SLVVLGGMGNIAGPVVGAVIWMTLLEWLRDFQFVQDHPEVRGMAMGLVLVTLMVFRPQ
                     GLMGSRRVALEMRPATEHESQQEREEVADVRRA"
BASE COUNT          674 a         1345 c         1153 g          653 t
ORIGIN      
        1 caggtcacgc gtcgatactc tcaacttgtc tgcaacatac gcctttgcct cggggagtcg
       61 catcttgcgg tggagttgca tcctggcgac cagatctccg gccgtgcgga tgccccccat
      121 cccggaggcg agcgcatgtg agatgggcat cccgtaggga tcgcccgaac ccacctataa
      181 cccgtccacc ctcgcgatat gcaccatggc gaccgaggcc cgcgtcacca tgctggtggg
      241 cggcgtctcg cacatcggca cgcctccgac gccgatcccc acgttcgcgt ggatggggat
      301 ggtcgccgcc tcgcagcagg ccttcacgaa ggtgacggcc cgcgccacgt tccaggccgt
      361 cgagcgccgt gtgttggtgt tcaccaccgg gccgaacacg tgagcgcccg ccttctccac
      421 cagcttgacc tgcttgtgcg ggtagaggcc cgccaagcgc accccgtcgt actccacttt
      481 cccgtgcatg ccgagcacga actcggcggc catcccgacc tcgatggaga ggtccgtggt
      541 gcgtgcgacc tcctccacgg ccaggagagt ggccaggaac tccgcgtcgc cggcggacgc
      601 ggtggtgtcg aagttgatgc cgtccgctcc caccgcagcc atgcgcttcg agacgtagac
      661 catgtccctc gtgcacatgg cagccgcctc ctcttgcgcc gcccgcgcct cgtcgatctt
      721 gcccatgggc aggaggtcgg ccgggttgcc gaaggggccg tcgggattgg cgtacagggc
      781 cagattcggc atcgccccgt agtacatggg gactatcgtc gaatgaagga cactctccat
      841 gcactgctgc tcaagcgcga tgatgggcct caccggcttg aacgagtagt ccgggtgccc
      901 cacctccatc gtgtccatag cgaaagcccg ctcgtagatg cggatgccca tctcgcggct
      961 caccgggacg cccaccccgc tcgaactctg agccccgtac atcgcgttca ccgagccgtc
     1021 gtcggtgagg accacctcgc taccgggttc cacccccacg atcttcccgg ggtaggtgaa
     1081 caggtcgagc aggtagtctc tctccccttc gctgagcgcc gggatccccc cgcgatcggc
     1141 ggcgtcctcg gtgcccgcta ggatatcggc gcggatctca tcagtggtca tctccaccgc
     1201 gtgtccgtcg cccatccgtg tcacatgtat agccatctgg atggcctcct ctctccaaga
     1261 ccgcgcctct tccgacagcg acccacttcc gcacccccgg cgcgctcgct cgcaccgagc
     1321 cgatcaggct ccgccgagat acgcctcctg cacctgggga ttcgagagga gcgcccgcgc
     1381 gtcgtcgctc aacacgatgg cgcccgtctc caggacgtag cctcggtgcg agatctcgag
     1441 ggccacgttg gcgttctgct ccaccagcag gatagtggtg ccctgcccgt tgatctcccg
     1501 gatgatctcg aagatcttct ccaccagcat gggcgccaga cccatcgagg gctcgtccaa
     1561 catgagaagc ttcggactgg ccatgagcgc acgaccgatg gccaacatct gctgctctcc
     1621 gccggatagg gtccccccgg cctgggtcct ccgctcgtgc agcaacggga agagaccata
     1681 gaccctgtcg aaatcggctc tcaggtcctg ccccgggcgc gagaaagccc ccatcgacag
     1741 gttctccttc accgacatgc gcgggaagat cttgcggccc tcgggagatt gagagaggcc
     1801 gagcttcacc gtctcgtacg cgggtacctt ggtgaggtcc gctccgtcat acacgatgcc
     1861 gcccgagcgc ggacgcaaca ggccagagat cgtgttcagc gtcgtactct tgccggcccc
     1921 gttcgcgccg atcagcgtga ctatctcgcc ccgctcgatg tacaaggaca cgcccttgag
     1981 agcgtggatg ttgccgtaga acacatgtat gtcggtgact tccaggagcg gcgggtggcc
     2041 gccggcctcg gcgcggccgc cgcccgactc ggcgccgagt ccctccatcc cgtctcgctc
     2101 cttcttcccc tcggccatca tgcactcttt ccaaggtatg cttcgacgac ccgggggtcc
     2161 gcgcgcacca cggcgggtgt cccctccgag atcttctcac cgtgatcgag cacgaacacg
     2221 gtgtcgcaga tgcccatgac cacgcgcatg tcgtgctcga tcagcacgac ggtcagcccg
     2281 gagtcgcgga tgcggcgcac caggcccacc agttcttgcg actcctgcgg attcattccg
     2341 gcagcgggct cgtccaggaa caggagcttc ggatcggtcg ccagggcgcg ggcgatctcc
     2401 aggcgacgtt ggcttccata gggcagattg cgcgccaact cggacacgct ccccttcaac
     2461 cccacgtact ccagcagttc gaccgatcga ctcctcacgc cccgctcctc ccgaagggag
     2521 ccgggagtgc gtgcgacaac gcccatgagc ccggtcttcg tgcggcagtg acgccccacc
     2581 atcacgttct cgagagccgt catctctccg aacaggcgga tgttctggaa ggtgcgcgcg
     2641 atgcccatcg acgtgatctt cgacggccga tgtccgccga tctcccgccc atcgaagagc
     2701 acccgacccg aagtgggcgt gtacacccct gtgatcacgt tgaagagcgt agtcttgccg
     2761 gctccgttcg gaccgatcag acccacgatc tcatccctat gcacggtcag cccgatcgag
     2821 ttcacggcca ccagcccgcc gaaagacttg gtgagggcct cgatctcgag gatggggccg
     2881 gcggagacag cggcatcagg cgcgcctgac atcagccacc tcctccctct cctgctggga
     2941 ttcgtgctca gtagccgggc gcatctccag agcgacgcgc cgcgatccca tcagaccttg
     3001 gggtcggaag accatcagag tcacaagaac caagcccatg gccatgccgc gcacctcagg
     3061 atggtcctgc acgaactgga agtcacgcag ccattcgagg agagtcatcc atatgaccgc
     3121 ccccacgacc ggaccggcga tattgcccat gcccccgagg accaccaggc tgagcaccat
     3181 gatcgactcc atgaggcgga agttgctggg gtggatgaag ttgaggtagt gcccgaggaa
     3241 ggcgcccatg accccggcga agaacccccc ggccgcgtag gccagcagct tgtagcgcga
     3301 ggtgtggatg cccatggcct ccgccgccac ttcgtcttcg cggatggccg tccatgcgcg
     3361 cccgatgcgc gaacgcacca tgcgcgagat cacgaagagc gagaggatcg ccagagccag
     3421 cgccaagtag tagaagcgga aggcggtgtt gaagtcgaag ccgaagacgc tcggcttggg
     3481 gatgcccgag atgccggagg gtccccgcgt gaggcccacc cagttgttcg ccacgatgcg
     3541 gatgatctcg tggaagccga gggtcatgat ggccaggtag tccccccgca ggcgcagggt
     3601 cggatagcct agtatcgctc cggcgacggc ggccaccact ccccccatga tcaggttgag
     3661 ccacacgagc caccacaccg aatccagctc gatgccccac gtgttgcgga cgagctgcac
     3721 catgatgatg gccgtgtagt acccaccgat ggcgtagaag gccacgtagc ccagatcgag
     3781 cagcccggtg aatcccacca cgatgttgag gcccgaagcc aagac
//
LOCUS       DASRMJ010000326         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_430984, whole genome shotgun sequence.
ACCESSION   DASRMJ010000326 DASRMJ010000000
VERSION     DASRMJ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430984"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..1573)
                     /locus_tag="VFD74_09015"
     CDS             complement(344..1573)
                     /locus_tag="VFD74_09015"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZK49721.1"
                     /translation="MTMHVYLLGLGAIGFVTSQDLAASPAFDRVTLADYSQKAVDRVA
                     ARIGERDHVAYTRIDAGDPAALEAAFKDVDLVINALEPKFNVNVMQACLRVGANYMDL
                     AQGGPRYVTGTPDSFEFLGWSQQFKDRGVLGILGMGMDPGVTNVFARYGADRMDEVFH
                     IGVRDGDSGVVPGMEDKFYTLWSPSIAIEECLMPAMIWENGEHKRTGIFGDPEVFPFP
                     EPLGPQTVYTVDHEEAKTLPLYIPGIREKGLKRCEFKYQLDEEFIEILKVLGKLGLNN
                     PFPVNVKGQMVVPRDVVVALMPDPAEVEGEAVGWGLIGSLIEGRHQGKDQKLFYYVKN
                     SYEACREEMGATAVAYQTGRPPAAMAELFAQGRLTIGGAAATGCYPCESFDPEPIVDA
                     LRAHGLTMEVADLTDAM"
     gene            complement(1625..2083)
                     /locus_tag="VFD74_09020"
     CDS             complement(1625..2083)
                     /locus_tag="VFD74_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZK49722.1"
                     /translation="MAEKGSLRRLDTADSQVLSLLSEDPRMSFAEMARRMGVSPGMVR
                     QRYQRLHDEGIVRIAAITNPTLTGGSLMVVVAMKVDVGDLKRITAEIAEFDEVVYLAV
                     LAGSFDLLAEARFRDQEHLHDFLSNRLRAIGGVRDSETYLNLEIIKETYY"
     gene            complement(2344..3546)
                     /locus_tag="VFD74_09025"
     CDS             complement(2344..3546)
                     /locus_tag="VFD74_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HZK49723.1"
                     /translation="MTEILVESFRDLVASTGFANITIGNVVMMVVGCVLVYLAIAKKF
                     EPLLLLPIGMGAVLTNIPAGGLFHSEIWLGPAGIAHGEWGRSEVFAALVKDGGLVDWL
                     YMGVKFGIFPPLIFLGVGALTDFGPLLANPKTILLGAAAQFGIFTTLLGALALGFTMK
                     EASSIGIIGGADGPTAIFLTSKLADHLMGPIAVAAYSYMALVPIIQPPIMRFIVPARE
                     RAIRMEQLRDVSRRVKIIFPVSVMVLVGLILPSAAPLIGALMLGNLFRESGVVDRLFG
                     AAQNELLNIVTIFLGVGVGATMTASNFLNWQTISILLLGLIAFSVGTAAGLLLGRLMN
                     AVSRTPINPLIGSAGVSAVPMAARVSQVVGQESDPDNYLLMHAMGPNVAGVIGSAVAA
                     GVLLAIAG"
     gene            complement(3550..>3807)
                     /locus_tag="VFD74_09030"
     CDS             complement(3550..>3807)
                     /locus_tag="VFD74_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZK49724.1"
                     /translation="TPVAAPVVLGGNAGAATGVVAPMPGKVLRVLVAEGDPVQRGTVV
                     AILEAMKMENELRASGDGTVRRIAVKESQNVSRGDLIVEVV"
BASE COUNT          697 a         1261 c         1214 g          635 t
ORIGIN      
        1 gggagttgtc gcgccaggat cgccttgatg gagcgaggta gtcgtgaccg catgcgcgga
       61 gcatccgccg cccacgaccg cgagagccag caccgagatg agcaggaagc ggaacgtcgt
      121 gcgtcgcacg ggatcctttc cccgacggat cgtcattcgg atggcgacgg ctgaggcggg
      181 gccgcgaagc gcagaatcgg ccggtgacgg gcccccgtcg ccaagagagt cgactcaagg
      241 caccttactg aggtggtctg aggttgtcaa ggcgcggagg caggacgacg gcgggggccc
      301 ggaggccccc gccgcacaga cagatccgga tgagccgctc cgactacatc gcgtcggtga
      361 gatcggccac ttccatggtg agaccgtgcg cacgcagcgc gtccacgatg ggctccgggt
      421 cgaagctctc gcagggatag cagcccgtcg cggccgcgcc gccgatggtg agacgcccct
      481 gggcgaagag ctcggccatc gccgccggcg gccggcctgt ctggtaggcc acagccgtgg
      541 cgcccatctc ttcgcggcag gcttcatagg agttcttgac gtagtagaag agcttctggt
      601 ccttgccctg gtggcgacct tcgatcaggc tgccgatgag accccagccc acggcctcgc
      661 cttccacctc cgccggatcg ggcatgaggg ccacgaccac atcgcgcggc accaccatct
      721 ggcccttgac gttcacgggg aaggggttgt tgagacccag tttgcccagg accttgagta
      781 tctcgatgaa ctcctcgtcc agctgatact tgaactcgca gcgcttcagc cccttctccc
      841 tgatgcccgg gatgtagagg gggagcgtct tagcttcctc atggtcgacc gtgtagaccg
      901 tctggggacc cagtggctcg gggaagggga agacctcggg gtctccgaag atgcccgtac
      961 gcttgtgctc tccgttctcc cagatcatgg cgggcatcag acactcttcg atcgcgatgg
     1021 aggggctcca caaggtgtag aacttgtcct ccatgccggg cacgacgccg ctgtcgccgt
     1081 cgcggacacc gatgtggaac acctcgtcca tacgatccgc gccgtagcgg gcgaacacgt
     1141 tggtcacgcc cgggtccatc cccatgccga ggatgccaag gacgccacgg tccttgaatt
     1201 gctgactcca gccgaggaac tcgaagctgt cgggagtgcc ggtaacgtac cgcgggccac
     1261 cttgggcgag gtccatgtag ttggcgccta cgcgcaggca cgcctgcatc acgttgacgt
     1321 tgaacttggg ttcgagcgcg ttgatgacca ggtccacatc cttgaacgcg gcctcgagcg
     1381 ccgcgggatc accggcgtcg atgcgcgtgt aggccacgtg gtcgcgctct ccgatgcgag
     1441 cggccacccg atcgactgct ttctggctgt agtcggccag cgtcacgcgg tcgaacgccg
     1501 ggctcgccgc gaggtcctgc gaggtcacga agccgatggc tcctaggccg agtagatata
     1561 cgtgcatagt catgctcctt ctcggtggtc ggcggaagac ggcgagcgcc gccccgcgag
     1621 gttcctaata gtaggtctcc ttgatgatct ccagattgag ataggtctcg gagtctctca
     1681 cccccccgat cgcgcgcaga cgattcgaga ggaagtcgtg gagatgctct tggtcgcgga
     1741 accttgcttc ggccaggaga tcgaacgacc cagcaaggac tgccaggtag acgacctcgt
     1801 cgaactcggc gatctctgcc gtgatccgct tgaggtcgcc aacatcgacc ttcatcgcca
     1861 cgaccaccat gagcgatccg ccggtcaggg tcggattggt gatggccgcg atccgaacga
     1921 tcccttcatc gtgcaggcgc tggtagcgct ggcgcaccat tccgggtgag acgcccatgc
     1981 ggcgggccat ctccgcgaac gacatgcgcg gatcctcgct cagcaaggag agaacctgcg
     2041 agtccgcggt gtcgagccgc ctcaaagatc ccttctcagc cacgcgctcg cctccccagg
     2101 tatcggccac gtgcgagggc gctatcaccc ccgcagccca gcaagcctat accgatagag
     2161 gagctgcgtc aagcatgttc gtgcctatac tgcacatact tctaagaatg atgctgtttc
     2221 ggcatagttc cgacgccacc actatcgatt cagcatcaca ggttacgggc ggtaagcgcg
     2281 gaatcgaagc gcgcgcggcg actgcaccac gaccagggcg aaggccgcct caccccggcc
     2341 cggctatccg gctatagcga gaagtacgcc cgccgcgacc gcggagccga tgaccccggc
     2401 gacattcggg cccatcgcgt gcatgagcag gtagttgtcg gggtctgact cctggcccac
     2461 cacctggctc acgcgggccg ccatcggcac tgcggagacg ccggccgacc cgatgagcgg
     2521 gttgatgggc gtgcgcgaca ccgcgttcat gagcctgccc agcagcagac cggctgccgt
     2581 ccccaccgag aaggcgatca atcccagcag aaggatggag atcgtctgcc aattcaagaa
     2641 gttggaggcc gtcatcgtgg cccccacgcc tactcccagg aagatggtga cgatgttgag
     2701 aagctcgttc tgagccgccc cgaacaggcg gtcgaccacg ccactttcgc ggaaaaggtt
     2761 gcccagcatg agcgcgccga tgagcggcgc cgcactcggg agtatgagcc ccaccagcac
     2821 catcaccgat accgggaaga tgatcttgac cctccggctc acgtcgcgca gctgctccat
     2881 gcggatggcg cgctcccgcg ccggcacgat gaatctcatg atgggcggct ggatgatggg
     2941 cacgagagcc atgtacgaat acgccgccac cgcgatcggc cccatgagat ggtctgcgag
     3001 cttggaggtc aggaagatgg cggtgggacc gtcggccccg ccgattatcc cgatggagct
     3061 cgcctccttc atcgtgaacc cgagtgccag cgcacccagg agtgtggtga agataccgaa
     3121 ttgcgcggcc gctcccagca agatggtctt cgggttggcc agcagcgggc cgaagtccgt
     3181 gagcgccccc actccgagga agatcagcgg agggaagatg ccgaacttga cgcccatgta
     3241 gagccagtcg accaggccgc cgtccttgac cagagcggcg aagacctccg agcgccccca
     3301 ctccccgtgc gcgatgccgg cgggacccag ccagatctca ctgtggaaga ggccgccggc
     3361 cgggatgttg gtgagcacgg cccccatgcc gatcggcaag aggagcagcg gctcgaactt
     3421 cttggcgata gccagataca ccaggacgca accgaccacc atcatcacga cgttgccgat
     3481 ggtgatgttc gcgaacccgg tggaggccac cagatcgcgg aaggattcca ccaggatctc
     3541 ggtcatgctc tacacgacct ccacgatgag gtcgcccctg gatacgttct gactctcttt
     3601 caccgctatg cgcctcaccg tgccgtcccc ggaggcccgc agttcgttct ccatcttcat
     3661 cgcctcgagt atggcgacca cggtgccacg ctgcacgggg tcgccctcgg ccaccaggac
     3721 ccgcagcact ttgcccggca tcggggccac gaccccggtc gccgcgccgg cgttgccccc
     3781 gagaaccacc ggcgcggcga ccggggt
//
LOCUS       DASRMJ010000327         5338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_280528, whole genome shotgun sequence.
ACCESSION   DASRMJ010000327 DASRMJ010000000
VERSION     DASRMJ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280528"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFD74_09035"
     CDS             <1..516
                     /locus_tag="VFD74_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZK49725.1"
                     /translation="LKSTKYDAIIGSMAITEKRLEQVNFTNPYYRSGAQIFVAEGNTD
                     INSKEDLAGKKIGVVKASTFKDIAVTLTDEDKVVGYDSDVIALQDLTTGRIDAVITDA
                     VVGFSAIMENGLKIKDVGEPLYIDEMGITVRKEDTALLADLNAALDAIIADGTYDRIS
                     QKWLGRNILGE"
     gene            615..1295
                     /locus_tag="VFD74_09040"
     CDS             615..1295
                     /locus_tag="VFD74_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZK49726.1"
                     /translation="MWEILQEHFVPFLEATGMTLKLTAVSLALAMVIGLAVSFLKLSQ
                     HRVLNWVAHVYIGVIRGTPLIVQLMFLYFGISAIVVLSSFWAGALALAIHSGAYVAEI
                     FRGTIQSIDKGQMEASRSLGVSHWKTMRLVILPQAFRRAVPPLGNQFIIGLKDSSLVA
                     YLGVAELYGTALSAQAENFMPFETYMVAGLYYLVLVLIFTALVQRLESRLDKANTGRQ
                     RDTRTRIA"
     gene            1270..1992
                     /locus_tag="VFD74_09045"
     CDS             1270..1992
                     /locus_tag="VFD74_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016112896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZK49727.1"
                     /translation="MIRVRGLHKYFGPLHVLKGIDLHIRKSEVVVLVGPSGSGKSTLL
                     RCLNFLELAQEGAIELLGRTVDPRKDDLDKVRENVGMVFQHFNLFPHMTALENITMAP
                     IHVKHQSKDEARAAARDLLERVGLADKEGSYPSQLSGGQQQRVAIARALAMNPDIMLF
                     DEVTSALDPELVGEVLQAMQDLAKSGMTMVVVTHEIGFAREVANRALMMDDGQIIEDA
                     APEILFTNPTHERTQRFLSQVL"
     gene            complement(2006..3727)
                     /locus_tag="VFD74_09050"
     CDS             complement(2006..3727)
                     /locus_tag="VFD74_09050"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZK49728.1"
                     /translation="MSITVREALELEPLAQGRLVAGRRGIDRVIRWVTIVEVLEDAGL
                     LQPGELLVTTAYDLENQPERRRTYIADLATRDLAGVAILTGFYIKEIPPEMIEQANEH
                     GLPLIQLPPSINFSDITRALLERIVNRQYELMHSSESVHRELTELALGDRDLADITSV
                     VARWTGGRVIFCNRRWDRVAAGGEADELHEVVMAAVQAEIGEDASDHPRELIVAEDQT
                     GSAYAALVVPVRASDRFFGNLVLIKLKGQLKDLDRVALGHASTVGALVLMRGEAVAEE
                     NRRLRGDLLDRILAGASAHNQTAVIDQARRFGCDLTKPHVAVVFRIRVVESQGRGGAL
                     AAADTSREAFESRRRSAGRLLRVVEEVVAGDGRSILVREREGDVLALIDATGLDEPVA
                     LARDVLTRWAAIDPGGPVVVAGVGEKESEVARFALSARQAQDAARLGPLLPGAPTLVE
                     HRDLGVFRLVADMERRHVDLDAAVRAGLGPLADDTPRSRRLLETLEAYLENDGNLQAT
                     AAALFVHRHTLRYRLERISDMMGRSLRDPLDRLELHLAVALHHYRRLRRAQAGEVDAA
                     APRVPPR"
     gene            3907..5229
                     /locus_tag="VFD74_09055"
     CDS             3907..5229
                     /locus_tag="VFD74_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZK49729.1"
                     /translation="MPLIQPLLDGVYPTVARGEGIFLYDTEGKRYLDGCSGAVTTGLG
                     HALPEIVRAMTEQAERVSFSYRLQFRNEPADELAALLEDLAPGDIRWTFFVNSGSEAT
                     ETAQKMAVQYWTESGRPEKNVVLSRNLSYHGITLGALSLSGHAARRRLFEPLLARNAV
                     VTPPHCYRCPLDKTYPGCDLACADDLERHIERLGDDRVAGFMVEPVIGAAGGAIVPPA
                     GYFARIREICDRHDVLLIADEVMTAMGRTGRMFAMEHWDVEPDLIAVGKGISAGYAPL
                     AAALASDRLVQTIRKGSGVIMSGHTYSGNPLSCAVGLAVMRYVLSHHLVERAAAAEAG
                     MRERLEALQRRHPMIGDVRGLGMMWGLELVADRSTRTPFPAAAQRAARVKEAAQDLGL
                     LVYPALSGADDRPGDSIIVAPPLTTTDAELDLLCDLLDQALEAVEKDS"
BASE COUNT          933 a         1851 c         1722 g          832 t
ORIGIN      
        1 ctcaagtcca cgaagtacga cgccatcatc ggcagcatgg ccatcaccga gaagcgtctt
       61 gagcaggtga acttcaccaa tccatactac cgctcgggcg cccagatctt cgtggccgaa
      121 ggcaacaccg acataaacag caaagaagac ctggccggca agaagatcgg cgtcgtgaag
      181 gcctccacct tcaaggacat cgccgtcacc ctcaccgatg aagacaaggt cgtgggctac
      241 gacagcgacg tgatcgccct gcaagacctc accacgggcc gcatcgacgc cgtcatcacc
      301 gacgcggtcg tgggcttctc cgccatcatg gagaacggcc tgaagatcaa ggacgtcggc
      361 gagccactct acatcgacga gatgggcatc acggtgcgca aggaagacac cgccctcctc
      421 gccgacctga acgccgccct cgacgcgatc atcgccgacg gcacctacga tcggatcagc
      481 cagaagtggc tgggacggaa catcctggga gaataggtgg gctaccccct cgtgtagcgg
      541 ggcgacgcgc cggcggacga ccgccggcgc gtcgccgtat cctctgacgc aaggagaaga
      601 cgcccgtgat cgggatgtgg gagatcctcc aagagcattt cgtacccttc ctcgaagcca
      661 cgggcatgac cctcaagctg acagcggtct cgctggcgct ggccatggtg atcgggctcg
      721 cggtctcgtt cctcaagctt tcgcagcatc gagtcctcaa ctgggtggcc cacgtgtaca
      781 tcggcgtgat ccgcggcaca cccctgatcg tacagctgat gttcctgtac ttcggcatca
      841 gcgcgatcgt ggtgctgagc tccttctggg caggcgccct ggccttggcc atccactccg
      901 gagcctatgt ggccgagatc ttccgcggga ccatccagtc gatcgacaag ggacagatgg
      961 aagcgtcccg ctcgctcggc gtctcccact ggaagaccat gcggctggtg atcctgcccc
     1021 aggcattccg acgcgccgta cctccgctcg gcaaccagtt catcatcggg ctcaaggact
     1081 catccctcgt tgcctacctt ggcgtggccg agctctacgg caccgccctc agtgcccagg
     1141 ccgagaactt catgcccttc gagacctaca tggtcgccgg tctctactac cttgtgctgg
     1201 tcctgatctt cacggccctg gtccagcggc tggaatcgcg cctggacaag gccaacactg
     1261 ggaggcagcg tgatacgcgt acgcggattg cataagtact tcggccctct ccacgtgctg
     1321 aagggcatcg acctccacat acggaagagt gaggtcgtgg tgctggtggg gccgagcggc
     1381 tccggcaaga gcacgctgct gcgctgcctc aacttcctcg aactcgccca ggaaggggcg
     1441 atcgaactcc tcggtcgaac cgtcgatcct cgcaaggacg acctggacaa ggtgcgcgag
     1501 aacgtgggca tggtgttcca gcacttcaac ctgttccccc acatgaccgc cctcgagaac
     1561 atcacgatgg cgcccatcca cgtcaagcat cagtcgaagg acgaggcccg cgccgcagct
     1621 cgggacctgc tcgagcgcgt cggcttggcc gacaaggagg gctcctaccc ttcgcagctc
     1681 tccggcggac aacaacaacg agtggccatc gcgcgcgcct tggcgatgaa ccccgacatc
     1741 atgctgttcg acgaggtgac gtccgccctc gatcccgagt tggtgggaga ggtgctccaa
     1801 gccatgcaag atctggccaa gagcggcatg accatggtcg tggtcaccca cgagattggt
     1861 ttcgcccgtg aggtcgcgaa ccgagccctc atgatggacg acggacagat catcgaggat
     1921 gcggcgcccg agatcctctt cacgaacccc acccacgagc gcacccagcg gttcctcagc
     1981 caggtcctgt gaattcggtt ctcaactacc gaggcggcac ccgcggggcg gctgcgtcga
     2041 cctctccggc ctgcgctcgg cgaagcctgc ggtagtgatg caacgccacc gcaagatgga
     2101 gttcgagtcg gtcgagcgga tcgcgcaacg agcgccccat catgtcgctg atgcgctcga
     2161 gccgatagcg cagggtgtgc cgatggacga acagcgcggc ggcggtggcc tgcaggttgc
     2221 cgtcgttctc gagataggcc tcgagcgtct cgagcaggcg gcgggaccgc ggagtgtcat
     2281 cggccagcgg cccgagaccg gcgcgcacgg cggcgtcgag gtccacgtgg cggcgttcca
     2341 tgtcggcgac caagcggaag accccgaggt cgcggtgctc cacgagggtg ggagcgccgg
     2401 ggaggagcgg acccagccga gccgcgtcct gcgcctggcg cgcactgagg gcgaagcgcg
     2461 cgacctccga ctccttctcg cccacgcccg ccaccacgac cgggccgccg gggtcgatcg
     2521 ccgcccaacg agtgagcacg tcgcgggcga gcgccaccgg ctcgtcgaga cccgtggcat
     2581 cgatgagcgc aagcacgtcg ccttccctct cccgcacgag aatactgcga ccgtcgcccg
     2641 ccaccacctc ttcgacgacc ctcaagagtc gccccgccga gcgccggcgc gactcgaagg
     2701 cctcacgcga ggtgtcggcg gcggcgagcg cccctccgcg cccctgggac tccacgaccc
     2761 gaatccggaa caccaccgcc acgtgcggct tggtcaggtc gcagccgaac cgacgggcct
     2821 ggtcgatcac ggcagtctgg ttgtgggcgc tcgccccagc gaggatgcgg tcgagcaggt
     2881 caccgcgcag acggcggttc tcctccgcca cagcctcacc gcgcatgagc acgagcgccc
     2941 ccacggtgga ggcgtgaccc agcgcgaccc gatcgagatc cttgagttgc cccttgagct
     3001 tgatcaggac caggttcccg aagaagcggt cgctggcccg cacgggaacc accagggcgg
     3061 cgtacgcgct gcccgtctgg tcctccgcca cgatgagctc ccgagggtga tcggaggcgt
     3121 cctctccgat ctcggcctgc accgccgcca tcaccacctc gtgcaactcg tcggcctccc
     3181 cgccggcggc tacgcgatcc caacgacggt tgcagaagat cacgcgcccg ccggtccacc
     3241 gagccaccac cgacgtgata tcggcgaggt ctcggtctcc caacgcgagc tcggtgagtt
     3301 cccggtgcac gctctcggag gagtgcatca gctcgtactg cctgtttacg atgcgctcca
     3361 ggagggcgcg cgtgatgtcg gagaagttga tgctgggcgg cagttggatg aggggcagac
     3421 cgtgctcgtt ggcctgctcg atcatctcgg gcgggatctc cttgatgtag aacccggtga
     3481 ggatggccac accggcgaga tcgcgcgtgg cgaggtcggc gatgtaggtg cgccgcctct
     3541 cgggctgatt ctcgagatcg taggcggtgg tgacgaggag ctcacccggt tggagaagcc
     3601 cggcgtcctc cagcacctcc acgatggtca cccaacggat gacgcgatcg ataccgcgcc
     3661 tgccggccac cagccgacct tgggccagag gttccagttc cagggcttcc ctgacggtga
     3721 tggacatggg actgcctgcc cccccgattg gccttcgcgg agtatcgaac ccgctgcgga
     3781 cggcattcca ccggcccgca ttgtacagat agtacaacag attccgctga ctctgcggac
     3841 tcctcctcac acaatcgaac cgcggcgacc cacaccgctg cgagaaccca gggagacgac
     3901 tgaaccatgc cactgatcca accactgctc gacggcgtgt atccgacggt ggcgaggggg
     3961 gaaggcatct tcctctacga cacggagggc aagcgctatc tggacggatg ctcgggagcg
     4021 gtcaccaccg ggttgggtca cgccctcccc gagatcgtgc gggcgatgac cgaacaggcc
     4081 gagcgcgtct ccttcagcta ccgccttcaa ttccgcaacg agcccgccga cgaacttgcg
     4141 gcgttgttgg aggacctggc gcccggcgac atccgctgga cgttcttcgt gaacagcggc
     4201 tcggaagcga cggagacggc gcagaagatg gccgtgcagt attggacgga gtccggcagg
     4261 ccggagaaga acgtggtgct ctcgcgcaac ctgagctatc acggcatcac tctcggagcg
     4321 ctctccctct cgggccacgc cgcccgacgc aggctgttcg agcccctgct ggcccggaac
     4381 gccgtggtga cgccacccca ctgctaccgg tgcccgctcg acaagaccta ccccggctgc
     4441 gacctcgcct gcgccgacga cctcgagcgc cacatcgagc gactgggcga cgaccgggtg
     4501 gccggcttca tggtggagcc cgtcatcggg gcggccggcg gggccatcgt gccgcccgcc
     4561 ggctacttcg cccgcatcag ggagatctgc gaccgccacg acgtgctcct catcgccgac
     4621 gaagtcatga ccgcgatggg gcgcaccggc aggatgttcg ccatggagca ctgggacgtc
     4681 gaacctgacc tcatcgcggt gggcaagggc atcagcgcgg gctatgcacc cctcgccgcc
     4741 gccctcgcct ccgaccgatt ggtgcagacc atccgcaaag gctccggcgt gatcatgagc
     4801 ggccacacct actcgggcaa ccctctgtcg tgcgcggtgg gcctggcggt gatgcgctac
     4861 gtgttgagtc accacttggt cgagcgcgcc gccgccgccg aggccggcat gcgggagcgg
     4921 ctcgaggcgc tgcagcggcg ccatccgatg atcggcgacg tgcgaggtct gggaatgatg
     4981 tgggggctgg agttggtggc cgaccgatcc acacgcactc cgttccccgc ggccgcgcag
     5041 cgcgccgccc gcgtgaaaga ggcggcacag gacttggggc tgctggtcta cccggccctc
     5101 tccggcgccg acgaccgccc cggggactcg atcatagtcg caccgccgct caccacgacc
     5161 gatgccgaac tcgacctctt gtgcgacctc ctcgaccagg cgctggaggc cgtggagaag
     5221 gactcatgag tggcgcgcga cctgggccga aggcggacct cgcccgcggc gccggcgggc
     5281 cggctgatct gccgggaggc gcgggcgggc cggccgatct ccgccgcggc gccggcgg
//
LOCUS       DASRMJ010000328         5251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_280550, whole genome shotgun sequence.
ACCESSION   DASRMJ010000328 DASRMJ010000000
VERSION     DASRMJ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280550"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFD74_09060"
     CDS             complement(<1..218)
                     /locus_tag="VFD74_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZK49730.1"
                     /translation="MDSDTILLIAVVAVALAFDLMNGFHDGCNAVSTVIYTGALKPKF
                     AIGLSAIFNFIGPLVMGVAVAETIGGVI"
     gene            371..1810
                     /locus_tag="VFD74_09065"
     CDS             371..1810
                     /locus_tag="VFD74_09065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZK49731.1"
                     /translation="MSHEPPRTYPGTSGDATAPSKGGSPLARARLALAAKLAFAAVLT
                     TILVFAAMDVHLGLALTITFIAATVALDYWVYRGASGLFKKEQTPDPRAVTHITGVHS
                     GLESLLQEVASGSSVAGGVEMETVRTDLLRLRDLNNQLIRLGDIAQELNAALPFKETK
                     VKTLGLSRDLLTADVVALVSEEGLEFSLEGVDGCEPSEIDVDCCTYYAPCPVRSAFRD
                     LSPAHTPDHRCLMFPPTMRSQLSLPFQLDEKRTWALVAAAARPDAFDEISPVVLQTLI
                     GHIQTSLSTALRYDRIRREVVTDPLTNLYNRRFFEQRAQEEIERCIRHHVPMTLMMLD
                     IDHFKRINDTYGHQTGDRVLQSVAQYLRESVRQTDICARYGGEEFILLLPATPGRNAV
                     FLADRLRRGLGDLMHTGLGLPSHESVTVSGGVATCPRDATDVEGLIAAADTALYQAKD
                     AGRNRIVRAELPDSRAAGEKNDNQASGTP"
     gene            complement(1869..2807)
                     /locus_tag="VFD74_09070"
     CDS             complement(1869..2807)
                     /locus_tag="VFD74_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZK49732.1"
                     /translation="MLSIFKTDLASGTLLELEDFEPGAWIDLVSPTDEELLRVKEALG
                     IPLAFLRGPLDAEEKSRIDAEDELTHVIVDIPIIERVGGERAYDTIPLGMLVHPDYFI
                     TTCLQPNPILGEFERGAVRSFATFKKTRFLLQILLQVSTYYLRYLGRIDRETDKIERE
                     LRQSMKNEELFDLLTLAKALVYFSTSLRSNDAVLQKLLRTPAIKKYEEDEELLEDVIV
                     ETRQAIEMAEVYSNILAGMMDAFASIISNNLNMVMKFLASITIILSFPVMVSSLYGMN
                     VPLPFQGDPHAFIFVLLVSLGLSTVAAVIFWRKDYL"
     gene            complement(2996..3193)
                     /locus_tag="VFD74_09075"
     CDS             complement(2996..3193)
                     /locus_tag="VFD74_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49733.1"
                     /translation="MTLYVDTSALIKAYVTEEASEDVLALIGEAEVVATSRLAYPVFA
                     CYDRRLNQAARVEGLQVFGPI"
     gene            complement(3376..3900)
                     /locus_tag="VFD74_09080"
     CDS             complement(3376..3900)
                     /locus_tag="VFD74_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49734.1"
                     /translation="MEMLVIVAIVAVVALVGMPTLRHTSVNAVLDGNMRNPAALVEEE
                     MLQGLDDTYHPAGDGDPEQYLSNQLEDVLGQAEGAARYINPRAGGPHSDAILNSASIL
                     ADPSPTAPAVFITNNPSSGYEVFDSQPYETSRQLLIGSLVVHFNTARRSVDVFFVSQN
                     GLKSAQVIRLPMDI"
     gene            complement(3990..4841)
                     /locus_tag="VFD74_09085"
     CDS             complement(3990..4841)
                     /locus_tag="VFD74_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49735.1"
                     /translation="METSASHRSQALVKAAKRAIARPWYLTIIAATVLCAVYLLEVLI
                     NWGHVAERSLYSNLGILPVGLAATLVTWSAARRRLDPRARTAWELLSISFGCFFAGDF
                     LYFYYQNVLGITPFPSLADAGYLVYYPLMFAGLICLPGRGSERARLGRLSVNLIVLIA
                     AGAAMVTYVYLVPMLGSPRDDLLAYVLSAGYPMGDLLLLGGLAALLVRRGEHRIQGSL
                     WVLGAGLMVGLGADVIFGYQTIHGALQAGGLCDAAYMLSWILFAWAGYAEFARVGREN
                     LQRGVSE"
BASE COUNT          968 a         1715 c         1715 g          853 t
ORIGIN      
        1 ttgatgaccc caccgatggt ctcggccacc gccacgccca tcaccagagg gccgatgaag
       61 ttgaagatcg cggagagacc gatggcgaac ttcggcttca gcgcccccgt gtagatgacg
      121 gtggagaccg cgttgcaccc gtcgtggaag ccgttcatga ggtcgaacgc cagcgcgacc
      181 gcgaccacgg ctatgagaag gatcgtgtcg ctatccaccg gcgatagctc gcttcgagca
      241 gcggatcacc cagttctctc cttcttcaga ttcgtatcac atgcgcgcca agcaccgaca
      301 atcatagaga ccgtgaccga gcgcgacaac tgctcacggt catgtccctc tcggtcgata
      361 caatcgttgt atgagccatg agcctccccg cacatatcct ggaacctccg gcgatgcgac
      421 cgctccctcg aaaggcggct cgccgctcgc gcgagcccgg ctggcgctcg cggccaagct
      481 ggcgttcgca gccgtgctca cgacgatcct cgtcttcgcc gcgatggacg tccacctggg
      541 gctcgcgctg acgatcacgt tcatcgcggc gaccgtggcg ctcgactact gggtctaccg
      601 gggggcttcg ggactcttca agaaggagca gaccccggac cctcgcgcgg tgacacacat
      661 cacgggcgtg cactcaggct tggagagcct gctgcaggag gtggcgtcgg ggtcctccgt
      721 ggccggaggg gtcgagatgg agaccgtgcg cacggacctc cttcgcctgc gcgatctgaa
      781 caaccagctc atcagactgg gcgacatagc gcaggaactg aacgccgcgc tccccttcaa
      841 ggagaccaag gtgaagacgc tcggcctctc gcgcgacctg ctgaccgccg acgtggtcgc
      901 cctggtgtcc gaggaggggt tggagttctc gctggaagga gtcgacggct gtgagccatc
      961 ggagatcgac gtcgactgct gcacctacta cgccccgtgc cccgtccgtt cggcgttccg
     1021 cgacctctcc ccggcgcaca cgcccgacca ccgctgcttg atgttcccgc ccacgatgcg
     1081 ctcgcagctg tcgcttcctt tccagctcga cgagaagcgg acctgggcgt tggtggcggc
     1141 ggcggcccgc cccgatgcct tcgacgagat cagcccggta gtgttgcaga cgctcatcgg
     1201 ccacatacaa acctctctct ccaccgccct tcgctacgac cgcatccgcc gtgaggtggt
     1261 caccgacccg ctcacgaacc tctacaaccg ccgcttcttc gagcaacgcg cacaagagga
     1321 gatcgagagg tgcatccggc accacgtccc gatgacgctg atgatgctcg acatcgacca
     1381 cttcaagcgc atcaacgaca cctacggtca ccagaccggc gaccgggtgc tgcagagtgt
     1441 ggcccagtac ctcagggaga gcgtgcgtca gaccgacatc tgtgcccgct acggcggcga
     1501 ggagttcatc ctgctcctgc ccgccacgcc cgggcgcaac gcggtgttcc tggccgaccg
     1561 gctgcgaaga gggctgggcg acctcatgca caccggtctg ggtcttccca gccacgagag
     1621 cgtgaccgtc agcggcgggg tcgcgacctg cccgcgcgat gccaccgatg tggagggcct
     1681 gatcgcagcc gcggacaccg ccctctatca ggcaaaggac gccggccgca acaggatcgt
     1741 gcgcgcggag ttgcccgact cgcgggccgc gggcgagaag aacgacaacc aggccagcgg
     1801 cacgccgtag cgcaacacgg gcccagccgg tgccgcgcgc gcgcccacga gcccgccctc
     1861 tacactctct acaggtagtc cttgcgccag aagatcacag cagcaacggt ggacagtccc
     1921 agcgagacaa ggagcacgaa gatgaaggcg tgcggatcgc cttggaacgg gagcgggacg
     1981 ttcatgccgt acagtgagga caccatcacc gggaacgaga ggatgatggt gatcgaggcg
     2041 aggaacttca tcaccatgtt caggttgttg ctgatgatcg acgcgaaggc gtccatcatg
     2101 cccgccagga tgttcgagta gacctcggcc atctcgatgg cctgccgagt ctcgacgatc
     2161 acgtcctcga gaagctcttc gtcctcctcg tacttcttga tggccggggt gcgcaggagt
     2221 ttctgcagga cggcgtcgtt ggagcgcagg gaggtgctga agtagacgag ggctttggcg
     2281 agggtgagaa gatcgaacaa ctcctcattc ttcatcgact gcctgagctc gcgctcgatc
     2341 ttgtcggtct cccggtcgat gcgccccagg tagcgcagat agtaggtgga cacctgcagc
     2401 aggatctgca gcaggaagcg ggtcttcttg aaggtggcga aactccgcac cgccccacgc
     2461 tcgaactcgc ccaggatggg gttcggctgg aggcaggtgg tgatgaagta gtcggggtgc
     2521 acgagcatgc cgagcgggat ggtgtcgtag gcgcgctcgc cacccacacg ctcgatgatg
     2581 gggatgtcca cgatcacgtg ggtgagttcg tcctccgcgt cgatgcggga cttctcctcg
     2641 gcgtccagag gaccgcgtag gaaggcgagg gggatgccca acgcctcctt gacccgcagg
     2701 agttcctcgt cggtgggact gaccagatct atccaggcgc cgggctcgaa gtcctcaagc
     2761 tccaataggg tgccagaggc aaggtccgtc ttgaagatgc tgagcatgac tcaccccctt
     2821 cacgggcgac ctgcgcgcgc ggcccggcga aggccgacgg gacggcctgg gtcggccgcg
     2881 cgatgggggc cgttcggacc gcctgccgga ggaccggcgc gtcactgaca tgacccgtgc
     2941 attctctcac gaacctgttg gtgacgctac gagagcagcg cggcgccgca ggcagctaga
     3001 tcgggccgaa cacctggagt ccctcgaccc gcgccgcctg gttcaaccgg cggtcgtagc
     3061 acgcgaagac agggtaggcg agtcgggatg tcgcgaccac ctcggcttcg cctattaaag
     3121 caagcacgtc ctcggacgcc tcctcggtga cgtacgcctt gatgagggcg ctggtatcga
     3181 cgtagagggt catgggccgc ggatcaggga tggggcgcgg gccacgggcc ggacgtcacg
     3241 ggtcgcgccg gtcctcgagc accgtgtcgg agatcggggg accgtccgcc cggacccccc
     3301 gcagacccac cggcatagtg tctcctgtgt cccgcctgag aacctgacta ggtcataata
     3361 gtgtggctat acaggtcata tgtccatggg caatcggatc acctgggctg acttcagacc
     3421 gttctggctg acgaagaaga cgtccaccga gcggcgcgcg gtgttgaagt gcaccactag
     3481 ggatccgatg aggagctgtc ggctcgtctc gtagggctgg gagtcgaaca cctcgtagcc
     3541 gctcgagggg ttgttggtga tgaacaccgc cggggccgtc ggcgaagggt cggccaggat
     3601 cgatgcgctg ttgaggatgg catcggagtg ggggccgccg gcccggggat tgatgtagcg
     3661 cgccgcaccc tcggcctgcc cgagcacgtc ctccagttga ttgctcaggt actgctccgg
     3721 gtcgccgtcg cccgccggat ggtacgtgtc gtccaatcct tggagcatct cctcttcgac
     3781 cagagccgcc gggttgcgca tatttccgtc aagcaccgcg ttcacgctcg tgtggcgcag
     3841 agtcggcatg ccaaccaacg cgaccaccgc gacgatcgcg acgatgacga gcatctccat
     3901 ggcgccgaag ccaccactac gacaacgttt ggagcaggaa gagtcgaggc ttcgaggaag
     3961 tcggcgggag ggcatgacag acgcccccat cactccgaga cgccccgttg gaggttctct
     4021 cgaccgactc gggcgaactc cgcataaccc gcccacgcga agaggatcca cgacagcatg
     4081 tacgccgcgt cgcacaatcc gccggcctga agtgcgccgt gtatggtctg gtatccgaat
     4141 atgacatcgg cgccaaggcc caccatgagc ccggcgccca gtacccagag cgaaccctgg
     4201 atgcggtgct cgcccctgcg gacgaggagc gcggccaggc cgccgagaag aagcaggtcc
     4261 cccatggggt agccggcgga gagcacgtag gccagaaggt cgtctcgggg ggatccgagc
     4321 atcgggacga ggtagacgta tgtcaccatc gcggcccccg ctgcgataag gactatcaag
     4381 ttcaccgaca gacgcccgag tcgtgcgcgc tcgctgcccc ggccgggcag gcagatcaga
     4441 ccggcgaaca tcagcgggta gtagaccaag tagcccgcgt cggcgagaga aggaaagggc
     4501 gtgatgccaa gcacgttctg gtagtagaag tacaagaagt cgcctgcgaa gaagcagccg
     4561 aaactgatgg agagtagctc ccaagctgtc ctcgctcgcg ggtcgagccg acggcgcgcg
     4621 gcgctccatg tgacaagtgt ggccgccaac cccacgggga ggatccccag gttcgagtag
     4681 agggaacgtt ccgccacgtg tccccagttg ataagcacct cgaggagata cacggcgcac
     4741 agtacggtgg cggcaatgat cgtgaggtac cacgggcgtg cgatcgcgcg cttggccgcc
     4801 ttgacaagcg cttgcgaccg atgcgaggcg cttgtctcca ctcccacgat ccgcgacctc
     4861 cggcttccga cctcggacca ggcttccgac ttcctacctg ggtgaccgcc cgtccgcgtg
     4921 actcgtagga tgttgagcgg ccaaccacct atcgggtgtt cggagcgcgg gcttgagccc
     4981 cgtgagaggg agtgccgacg aggccggccg cggagctcgt gcaccgcccc attctcgggc
     5041 ccggctgcgg ctgaaggccc cacccccgct tcttccgaag cccccgaccc gatgttagta
     5101 tgagcctcgg cgccgccggc cggcggcgcc gaggctcgga agacgaggct acacctatgc
     5161 gacccaccct gaccgcgatc gagcgcgacc tacgcgggct cctgcgagga gacgtggagt
     5221 tcgacgctgt gacccggcac ctttacgcca c
//
LOCUS       DASRMJ010000329        16366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_249702, whole genome shotgun sequence.
ACCESSION   DASRMJ010000329 DASRMJ010000000
VERSION     DASRMJ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 16366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16366
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249702"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFD74_09090"
     CDS             <1..636
                     /locus_tag="VFD74_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZK49736.1"
                     /translation="GHTLLVRVDGPAAFTVLGQTLDDAAGEAFDKGARLLGLGYPGGR
                     ELDMLAETGDAAAYDFPIGLKRSSSLDFSFSGVKTSLFYLLRSWSAEERAERAADVAA
                     SYRQAVVEALVSKLFAAADREAVPAIAIGGGVAANSLLRRRLAAEGPARGLHLVLPPL
                     KYCTDNAAMIGAAALSGPVLPFPEYLALEASASLPLWVTPRGDTPRFSPNR"
     gene            786..1070
                     /gene="groES"
                     /locus_tag="VFD74_09095"
     CDS             786..1070
                     /gene="groES"
                     /locus_tag="VFD74_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZK49737.1"
                     /translation="MKIVPLEDRVVVRVLEEEEKTASGIVLPDTAKEKPQKAKVVAVG
                     PGRYDDGALVPMGVAEGDTVIFSKYGGTEVKIDGGEVLILRASDILAKVE"
     gene            1175..2806
                     /gene="groL"
                     /locus_tag="VFD74_09100"
     CDS             1175..2806
                     /gene="groL"
                     /locus_tag="VFD74_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932107.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZK49738.1"
                     /translation="MAKEIKYGEDARRALERGVNQLADAVKITLGPKGRYVVLDKKFG
                     SPTITNDGVTIAREIELEDVFENQGAQLVREVATKTNDIAGDGTTTATVLAQAIVREG
                     LKNVAAGANPMAIKRGIEKAVTAAVDAIKAQSKEITGKSDIARVATISADDSEIGDVI
                     ADAIEKVGKDGVVNVEESNTFGMDLEFTEGMQFDKGYLSPYFVTDPERMEAVLEDPFI
                     LIANQKIGSVQDLLPVLEKTVQSGKSLLLIAEDVEGEALATLVVNKLRGTFTGIAVKA
                     PGFGDRRKRMLEDIAILTGGEVITEELGLKLENTQTTQLGHARKIVVTKDNTTIIDGA
                     GDVEQIKGRINQIKAEIENTDSDFDREKLQERLAKLAGGVAVVKVGAATETEIKEKKH
                     RVEDALSATRAALEEGIIPGGGVALVLAIPAVQAVEASGDEKTGVNIIARALEEPLRQ
                     LANNAGLEGSVVVNEVKVRGKGIGLNVATGEYEDMIGAGIIDPAMVTRSALQNAASIA
                     KNILTTEVIVADKPEESGGMGGMGGGGMGGMGGMM"
     gene            3074..3502
                     /locus_tag="VFD74_09105"
     CDS             3074..3502
                     /locus_tag="VFD74_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49739.1"
                     /translation="MKEQAEKAFKEMLEISTDLDKAVLYSGEEAVVSNFPDGLQATMV
                     KKAKELSAAGAVRAASMGSAPLTQMVVESAGGTVFLVHEPQDDGLGMLATAKKDSRIG
                     LVFYDLKTCIRDAQEVDEEPIDEEPIDEGPEADAGAEVEA"
     gene            3502..3717
                     /locus_tag="VFD74_09110"
     CDS             3502..3717
                     /locus_tag="VFD74_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49740.1"
                     /translation="MKKLLKFFGFVSILGSAAVGVYYFFFMRKKKPSVELYFDDGSML
                     AFDESASEAGEFLALADDVLALSPVIG"
     gene            3764..5164
                     /locus_tag="VFD74_09115"
     CDS             3764..5164
                     /locus_tag="VFD74_09115"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZK49741.1"
                     /translation="MSGQSPGLTPALADVVLARVLRHGGDYAEIFAEDRSSLSVRLDD
                     RRLEEVSSGVDRGASIRLVKGPTTSCGYVDALDEGSLIGLADRLSAGVSGGPLLPVSL
                     NALQPLPRRQAGGDPAAMPAPVKADMLRAVDAAARGRSAEVRQVAAGYSESRQRIWVA
                     GSRGVFAHDDRTRMVLSVNVVAQRGDVIQTGRETLSGTTGFGLFEDGSPVLAALEATE
                     KALVMLESRPAPSGRMPVVLANGFGGVLFHEACGHGLEADYIVKKTSIWEGKRGTRVA
                     EPFVTALDDGVTPGLWGSNSIDDEGTNTEATVVIEEGVLTGYLTDLLRGERLGMASTG
                     NGRRQGFRHLPYPRMTNAYFAAGAVTVADLIADTPRAFYAKSLSGGQVDPATGDFVFG
                     VSEGYLIEGGRVGPAVRGATLIGNGAEVLRSIDAIADDLVVKAGMCGKEGQAVPVGTG
                     QPTLRIRELTVGGTAV"
     gene            5164..6534
                     /locus_tag="VFD74_09120"
     CDS             5164..6534
                     /locus_tag="VFD74_09120"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZK49742.1"
                     /translation="MGRSDPADLVVVGRDVLRRGKRFPGCLEVFVEHSETTSIKAYQG
                     EVESLVTATPRGLGVRYVEGSRCGYAYTGDFSSSGLDLVVSMAVSNAAVSDADESVAL
                     PEAPSAYPEIPGLWRPALLDTPMEKKIDLALEAERVALASPHIETVEESSYVDQASRV
                     AILSSRGVEVYGGQTFCYVYLSAHARSGDEVQTGLGFETGREPSELDASAAGREAARK
                     AAALLGARPHPTGRYTVVLDREVAAAVIGIAAQALSADAVQKGRSLFAGRVGQRVAAD
                     AFTLVDDGLHRDGMETAPFDDEGVPRGITPLVENGLLRGFLHDTRTAARAGGGVRSTG
                     NARRAGYRSSPGVSPSNLVLTPGQGTLSDLVARVESGVYIVGITGLHSGANPVTGEFS
                     VGAFGHVIEGGAIGTPVREITIASDLVSLLTNVSDSAGDVRWIPFHGSVSTPSLAILD
                     VTVAGS"
     gene            6531..7130
                     /gene="pyrE"
                     /locus_tag="VFD74_09125"
     CDS             6531..7130
                     /gene="pyrE"
                     /locus_tag="VFD74_09125"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZK49743.1"
                     /translation="MNRASLGRLLVAVAYLEGDFVLRSGRHSRYYLDKYLFETEPRVL
                     RGIVNEMALMIRNRMAAGVVYDRLAAPELGAVSLGAGLSLESGIPFLIVRKGEKGYGT
                     SKAIEGAFVPRDRVALVEDIVTSGGAALAAVEKLRQVGLVVEDLFCVVDREEGGAEAA
                     LAAGLTLRPLFTASGLGIVVGSADERSDRGDPTGGEPAS"
     gene            7239..7511
                     /locus_tag="VFD74_09130"
     CDS             7239..7511
                     /locus_tag="VFD74_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZK49744.1"
                     /translation="MTKSDFVEKLAAKADLSKKDAAAAVDAFVDVVSEALAAGESVQF
                     TGFGKFYTQQREAREGINPQTKAKIQIAATTVPKFSAGSGLKSAVR"
     gene            7630..8532
                     /gene="pyrF"
                     /locus_tag="VFD74_09135"
     CDS             7630..8532
                     /gene="pyrF"
                     /locus_tag="VFD74_09135"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZK49745.1"
                     /translation="MKNFADRLLGAVEDRRSHVVVGLDPDYSLLPPELRTLVPSQVAD
                     ADALAECFDVFLRGILGAVAGIAVAVKPQLAYFEALGSAGYGLYERIVAEAREQGFLV
                     IADAKRGDIGSTAEAYARAHLDVVGADAVTVNPWFGTDGIEPFILRTKDDGRGLFVLV
                     KTSNPGSAEIQDLVLAAGGSPVFEHVAGLVDTWGVGAVGECGYSSVGAVVGATHPEQA
                     ARLRVAHPTTPFLVPGFGAQGAGPSQLSGIFDAGGTGAVVNSSRAILYAYRARGGDWR
                     AAARDEAEEMRRALWAVAHGGGES"
     gene            8529..9053
                     /locus_tag="VFD74_09140"
     CDS             8529..9053
                     /locus_tag="VFD74_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZK49746.1"
                     /translation="MSGAVGRVLAPLAALALVVLLVSLVGGSDLLAGVDRPEGPETPE
                     TSRTTIIYLTEPGGNQADAGAGAGAGSAGGASVSAPGSDSSATAGSEGASTGGAASGA
                     TGGAASSTTGSQSTGVSTDPAAQETTYTVQAGDTPYSIGLAFGVSTQELMRANGISDP
                     TNLQVGTVLVIPAK"
     gene            complement(9121..9456)
                     /locus_tag="VFD74_09145"
     CDS             complement(9121..9456)
                     /locus_tag="VFD74_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49747.1"
                     /translation="MGQGLPERYRVDEIAVRHQERALAVTAGRIGGAEIRPTRVFLWV
                     GTRTAGRERMISMDSQPHLLRSGRRRHLDLGACHVSFLGNPLLTLGLFAVKAVRRAAG
                     GRSGATPAA"
     gene            complement(9460..10197)
                     /locus_tag="VFD74_09150"
     CDS             complement(9460..10197)
                     /locus_tag="VFD74_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49748.1"
                     /translation="MRSAWARLTHKQLLFVYPLILGVINVVAFFAVYASVGGRLSFSE
                     FAQTDFTRWTFLQEHIGDLAGGLDHALIALLAGAAICLLSAAVRAPYFRAIAGSSYPR
                     SPRSTGEFVRLAQFYAFTYAVFYIVPYLVAVESAAGQALSLAVLPLAILLVFGDYALV
                     FESLSPIAAAKRSIKLLRRGWMMVIPIYLVALLLWSGSFALFDRYYQSADGVFPLFVV
                     SQLLVEALITLLLDVVLIFTYDHLRRL"
     gene            10408..11682
                     /locus_tag="VFD74_09155"
     CDS             10408..11682
                     /locus_tag="VFD74_09155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZK49749.1"
                     /translation="MGATLLLLLWLLLGGSGFPGAPGATEALSATTTTGQTAQVGQTG
                     STQHQVSSEVLPPAATQDPKAAADRVPAVGGKSVILVNMDDGRVIYERNADEQRSIAS
                     TTKIMTGIIALETLPLDKIVTASAKATDAGESEIWMEPGEQLSVGDLMKALLVRSAND
                     AAVALGEAAAGSLDAFVEKMNAKAAELGLINTHFANPHGLEAKEHYSSARDLAKLARY
                     AMQNEEFRKLVATAKVNIPWPGRPYDRVLKNRNDLVGAVQFVTGVKSGYTAKAGYCLV
                     GSGARDGASLVSVILGEDTKAAVDADTVSLLEYGFTKYRQVSLMEEGVPVAAMDVPYH
                     FGEKVVLKTERNLVRTVYSDDPITKTVVVQDRLVLPVSQGQVLGKITFATGTAVAGEV
                     DLVSDRAIEAPTLRVKLRYVWDRMINWLGKAV"
     gene            11690..12628
                     /locus_tag="VFD74_09160"
     CDS             11690..12628
                     /locus_tag="VFD74_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HZK49750.1"
                     /translation="MILTVTLNAAVDRTLTVPNFEVGFRHRASETITLPGGKGINVAR
                     VVKTLGQPVIATGFAGGRTGDRIISDLNREGILSDFVRIEGVSRTSTAVLDPTTNRTT
                     EINEYGPDISPRELELMLEKIDYLMKAADLVIVAGSLPSGLPTSFYGELIPRVRAAGV
                     PVLFDTWGEALRLGIKASPDVVFPNQVEAEMVVGYEFSSNEDFIRAAENLRDLGARSA
                     VIKSKMGCVAKIKYNGDVHTLMGKAPKVEAVSTVGSGDSIVGGYAAKLLEGAPPEDCL
                     RFGLACAAANALRPGAGVFDPNDAARLAPGVEMEEV"
     gene            12625..14181
                     /gene="guaA"
                     /locus_tag="VFD74_09165"
     CDS             12625..14181
                     /gene="guaA"
                     /locus_tag="VFD74_09165"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZK49751.1"
                     /translation="MSGMTGRDEPPHKVLVLDFGGQYAQLIARRVRECRVYSELMPFD
                     TPLSELMAADPAGIILSGGPRSVNDAGAPVPDPGILELGVPVLGICYGLQLLSKLSGG
                     VVARTDRAEYGGMDIEVVADSALFEDLPRDQPSWMSHGDAVLETPPGFTLTARSRSLP
                     VAAVEDVRRHLYGVQFHPEVRHTRHGQDILKNFLYRICDIAPTWTPVSIIEDAVARIR
                     EQVGDGRIICGLSGGVDSAVAALLTYKAVGDQLTCIFVDHGLMRKDEGEQVESAFGYH
                     FHVPLVHVRAQERFITKLAGVQDPETKRKIIGEEFIRTFEAEARRLGDVRFLVQGTLY
                     SDVIESGTRDAAKIKSHHNVGGLPEDMDFDLVEPLRMLFKDEVRVVGEELGLPDSMVW
                     RQPFPGPGLAIRIIGDVTEERLHILREADAIILDEVRKAGLYRELWQSFAVLPAIRSV
                     GVMGDERTYAYPIVIRAVTSEDAMTADWARLPYELLERISSRVINEVPGVNRVALDVS
                     SKPPSTIEWE"
     gene            14190..14789
                     /gene="rsfS"
                     /locus_tag="VFD74_09170"
     CDS             14190..14789
                     /gene="rsfS"
                     /locus_tag="VFD74_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZK49752.1"
                     /translation="MTDETMRPEDHDEGEDAPEVIMVTGPEGEDARLEPNEDLDVDLE
                     ARGIADLPADETTDPESQEGPRALGVPVRTQPRERSTDDKLELIHRHTLLAAATASGM
                     KAEDIRLLDVHELVSYTDFLLICTGRSARQTGRIAEEIALKLKQELGLIPSSVEGERP
                     GEWVLLDYLDFIVHVFTPEAHDFYRLDVLWKQAPTEIIE"
     gene            14980..15055
                     /locus_tag="VFD74_09175"
     tRNA            14980..15055
                     /locus_tag="VFD74_09175"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15013..15015,aa:Ala,seq:ggc)
BASE COUNT         2851 a         5004 c         5716 g         2795 t
ORIGIN      
        1 ggacacacgc tccttgtgcg cgtggacgga ccggcggcct tcacggtact cggtcagacc
       61 ctcgacgatg ccgccggtga ggcgttcgac aagggcgccc gccttctcgg actcggctat
      121 cccggcggaa gggagctgga catgctcgcc gagaccggag acgcagccgc gtacgacttc
      181 ccgatcgggc tgaagcgctc gagcagtctc gacttctctt tctccggcgt caagacctcg
      241 ctcttctacc ttcttcgctc ctggtccgcg gaggagcgcg ccgagcgtgc cgccgatgtg
      301 gcggcgtcgt atcgtcaggc ggtggtggaa gctctggtct ccaagctgtt cgcggcggca
      361 gacagggaag cggtgcccgc gatcgcgatc ggcggcgggg tcgcggcgaa ctcgttgctg
      421 cggcggcgcc tggcggcgga agggccggcg cgaggtctgc acctggtatt gccgcctctc
      481 aagtactgta cggacaacgc ggcgatgatc ggagcggccg cgttgtcggg cccggtgctt
      541 cctttcccgg agtacctggc gctggaggcc tcggcgtcgc tccctctgtg ggtgactccc
      601 aggggcgata cgccccgctt ctcccccaac cggtagcggt cccccgtttg acattttctg
      661 agtgggggca tatcatgtcg cacagattgg cactctcctt gcgagagtgc caattgtgac
      721 gttacgagtg catcggcgcc gaatgggcga cggctcagag caaaggatga caggagggtt
      781 cgtgcatgaa aatcgtgccg cttgaggaca gggtcgtagt cagggtcctg gaagaagaag
      841 agaagacggc cagcggtatc gttctgccgg atacggcgaa ggagaagccg cagaaggcca
      901 aggtagtggc agtaggtccc ggtcgctacg acgacggcgc cctggttccc atgggcgtgg
      961 ccgaaggtga caccgtcatc ttcagcaagt acgggggcac cgaagtcaag atcgatggcg
     1021 gcgaggtgct gatcctgcgc gcgtcggaca tcctggcgaa ggtcgagtag tagcagcacg
     1081 atgatggatg gcgctgccga gccggacgac accgcgctga gtggacaggc ccggggcgcg
     1141 caggtacaga gcagctaagg aggctgacag actcatggca aaagagatca agtacggcga
     1201 ggatgcccgc agggccctcg agcgcggcgt gaaccaactc gccgacgcgg taaagatcac
     1261 tctcggtccc aagggccggt acgtggtgct cgacaagaag ttcggcagcc cgacgatcac
     1321 gaacgatggt gtgaccatcg cccgggagat cgagctcgag gacgtattcg agaaccaagg
     1381 cgcccaactg gtgcgcgaag tggccaccaa gaccaacgac atcgccggtg acggcaccac
     1441 caccgctacg gtgctggcgc aggccatcgt acgcgaaggc ctcaagaacg tggctgccgg
     1501 tgccaacccg atggccatca agcgcggcat cgagaaggct gttacggctg cagtcgatgc
     1561 gatcaaggct cagtccaagg agatcaccgg caagtccgac atcgctcggg tcgccaccat
     1621 ctccgccgac gactccgaga tcggcgacgt catcgccgat gccatcgaga aggtgggcaa
     1681 ggacggcgtt gtcaacgtcg aggaatccaa caccttcggc atggacctgg agttcacgga
     1741 aggtatgcag ttcgacaagg gttacctctc gccgtacttc gtcaccgacc cggagcggat
     1801 ggaagcggtg ctcgaggatc ccttcatcct gatcgccaac cagaagatcg gttcggttca
     1861 ggacctgctt cccgtgctcg aaaagaccgt ccagtccggt aagtcgctgc tgctcatcgc
     1921 cgaggacgtc gagggcgaag ccctggccac gctcgttgtg aacaagcttc gcggcacgtt
     1981 cacgggcatc gcagtcaaag ccccgggctt cggcgaccgt cgcaagcgta tgcttgaaga
     2041 catcgccatc ctcacgggcg gcgaggtcat caccgaggaa ctgggtctca agctcgagaa
     2101 cacccagacc actcaacttg gtcacgcccg caagatcgtg gtgacgaaag acaacaccac
     2161 catcatcgac ggtgccggcg acgtggagca gatcaagggc cgaatcaacc agatcaaggc
     2221 cgagatcgag aataccgact ccgacttcga ccgcgagaag ctgcaagagc gcctggccaa
     2281 gctggccggc ggcgttgcgg tggtcaaagt aggcgcggcc acggagaccg agatcaaaga
     2341 gaagaagcac cgtgtagagg acgccctttc ggcgactcgt gcggctctcg aagaaggcat
     2401 catccccggc ggcggcgttg cactggttct ggccatcccg gccgtccagg ctgtagaagc
     2461 ttccggcgac gagaagaccg gcgtgaacat catcgcgcgc gccctcgaag agcctcttcg
     2521 tcagctggcc aacaacgccg gcctcgaagg ctcggtcgtg gtcaacgagg taaaggtgcg
     2581 cggcaagggc atcggcctca acgtcgccac gggcgagtac gaggacatga tcggcgccgg
     2641 tatcatcgat ccggccatgg tcacgcgctc tgctctgcag aatgcggctt cgatcgccaa
     2701 gaacatcctg accaccgaag tcatcgtggc cgacaagccc gaagagtccg gtggcatggg
     2761 cggcatgggc ggcggcggca tgggcggcat gggcggcatg atgtaagaaa cccactcgcg
     2821 agcatccctc ggcaccaagc gtcgagcgat gttaccgatg tggtcctgtg ggggccgcga
     2881 ccgtctcggt cgcggccccc actgtcgttc cgggccaggc acggccctgg gcgtccctgg
     2941 ccccgagccg tgcctggccc agcgccgtcc gaagcggtag tgatacactt ggtcgtgcta
     3001 ccagcgtcgt acgcacaaga cctgaacttg gctggacgag agagtcgctt tcggccggag
     3061 aaaggagtgc cgcgtgaagg aacaggcgga gaaagccttc aaggagatgc tcgagatctc
     3121 cactgacctg gacaaggccg tcctctattc tggtgaggag gcagtggtgt cgaacttccc
     3181 cgacggtctc caggcgacga tggtcaagaa ggccaaggag ttgtcggcgg cgggcgccgt
     3241 tcgggccgcg agtatgggat ccgcgccgtt gacccagatg gtggtggaga gtgccggagg
     3301 cacggtcttc ctggtgcacg agcctcagga cgacggcctg gggatgctcg ccacggccaa
     3361 gaaagatagt cgcatcggac ttgtcttcta cgatttgaag acctgtatcc gtgacgcaca
     3421 ggaggtcgac gaagagccga tcgacgaaga gccgatcgac gaagggccgg aagcggatgc
     3481 cggagcggag gtggaggcgt aatgaagaag ctgcttaagt tcttcggctt cgtgtcgatc
     3541 ctcggctccg cggccgttgg cgtctactac tttttcttta tgcgcaagaa gaagccgtca
     3601 gtggagctct acttcgacga tggatccatg ttggcgttcg acgagagcgc ttccgaagcc
     3661 ggcgagttcc tagcgttggc cgacgatgtc cttgccctca gtccggtgat cggctgaagc
     3721 gtctgcagcc gatcggcctt gtgcccagtc ttggatgggt agcgtgagcg ggcaatcgcc
     3781 gggattgact cccgcgctgg ccgacgttgt gttggcgcgc gtcctgcgtc acggcggcga
     3841 ctacgcggag atcttcgccg aggacagatc ctccctgtcg gtccgattgg acgaccggcg
     3901 cctggaggag gtctcctcgg gcgtcgaccg gggcgcttcg atacgcctgg tgaaggggcc
     3961 cactacgtcg tgcggctatg tggacgccct ggatgaaggg tccctcatag gactcgccga
     4021 tcgcctgagt gccggcgtga gtggcgggcc cttgctgccg gtctcgctca acgcattgca
     4081 gccgttgcca cgccggcagg cgggcggcga cccggccgcc atgcctgccc ccgtcaaggc
     4141 cgacatgttg agggcggtgg atgcagcagc ccgggggcgg tccgcggagg tgcgtcaggt
     4201 cgcggccggc tactccgaga gcaggcaacg catctgggtc gccgggtcga ggggcgtgtt
     4261 cgctcacgac gatcgcaccc ggatggtgct cagcgtgaac gtagtggccc agcgggggga
     4321 tgtcatccag acgggccgag agactctgtc cggcaccacg gggttcgggc tgttcgaaga
     4381 cggctctcct gtgctggcgg ctctggaggc cacggagaag gccctggtca tgctcgaatc
     4441 ccggccggct cccagtggtc gcatgccggt ggtgctcgcc aacgggttcg ggggcgtgct
     4501 cttccatgaa gcctgcgggc acggcctgga agccgactac atcgtcaaga agacgagcat
     4561 ctgggaaggc aagcgtggca ccagggtggc cgagccgttc gtgaccgcgc tcgacgacgg
     4621 cgtcacgccc gggctgtggg gcagcaactc catcgacgac gaggggacga acaccgaggc
     4681 cacggtcgtg atcgaggagg gtgttctcac cggctacctc accgacctgc tccgggggga
     4741 gagactcggg atggcatcga cggggaacgg caggcgccag ggcttccggc acctgcccta
     4801 tccgcgcatg acgaacgcct acttcgccgc gggcgccgtc accgtcgccg acttgatcgc
     4861 cgacactccc agggccttct acgccaagag cctttcgggt ggccaggtcg atccggcaac
     4921 tggggacttc gtgttcggcg tctccgaggg ctacctgatc gaaggcgggc gtgtgggacc
     4981 ggcggtgcgt ggggctactc tcatcggcaa cggagccgag gtcctgcgtt ccatcgacgc
     5041 gatcgctgat gacttggtgg tcaaagccgg gatgtgcggc aaggagggcc aggcggttcc
     5101 cgtgggcacc ggccagccca cgttgcgcat ccgagagttg accgtcggcg ggacggcggt
     5161 gtgatgggcc ggagcgatcc cgcagatctg gtggttgtcg gaagggacgt tcttcggcgt
     5221 gggaagcgct tcccggggtg ccttgaggtg ttcgtggagc actcggagac gacttccatc
     5281 aaggcctacc aaggtgaggt cgaatcgctg gtcacagcta cgccgcgtgg actcggtgtg
     5341 cggtatgtgg agggatcgcg ctgcggctac gcatataccg gcgacttctc atcgtctggc
     5401 ctcgacctgg tggtgtccat ggcggtctcg aacgccgccg tctccgacgc ggacgagtcc
     5461 gtcgcgctcc ccgaggcgcc ttcggcctat cccgagatcc ccggcctgtg gcggccggcc
     5521 ctgctcgaca cgccgatgga gaagaagatc gacttggcgc tcgaggcaga gcgtgtcgcg
     5581 ctggcctcgc cgcacattga gaccgtcgag gagagcagct acgtggatca ggcctcgcgc
     5641 gtggccatcc tctcgagccg cggggtggag gtctacggcg ggcagacgtt ctgctacgtg
     5701 tatctctctg cccacgcccg cagcggcgac gaagtgcaga ccggcctcgg cttcgagacc
     5761 ggccgcgagc cgagcgaact cgatgcctcc gccgccggcc gggaagcggc ccgcaaggcc
     5821 gccgctcttc tcggggcccg tccccatccc accgggcgct acacggtggt gctcgatcga
     5881 gaggtggctg cggccgtgat cgggatcgcg gcgcaggcat tgagcgccga cgctgtgcag
     5941 aaggggaggt cgctcttcgc cggacgggtg ggacagcgcg tggccgccga cgccttcaca
     6001 ctggtggatg acggcctgca ccgcgacgga atggagacgg cgcctttcga cgacgaagga
     6061 gtgccgcggg ggatcacccc gctcgtggag aacggcctgc tccgcggctt cctgcacgac
     6121 acacgcaccg ctgcgcgcgc aggcggcgga gtgcgctcca cgggcaacgc tcggcgcgcg
     6181 ggctatcggt cgtcacccgg ggtctctccg tcgaacttgg tgctgacacc cggccagggg
     6241 actctgagtg atctcgtggc gcgggtggag agcggcgtct acatcgtggg gatcacgggt
     6301 cttcactcgg gagcgaaccc ggtgaccggg gagttctcgg tgggagcgtt cggccacgtc
     6361 atcgaaggcg gagccatcgg tacgccggtg cgggagatca cgatcgcctc cgacctcgtc
     6421 tcgcttctta ccaacgtgtc cgattcggca ggcgacgtgc gctggatacc cttccacggg
     6481 agcgtgtcca cgccttcact ggctatcctc gacgtgacgg tggcgggctc gtgaacaggg
     6541 cgtctctggg aaggctcctg gtggcggtgg cctacctgga gggtgacttc gtgctgcgct
     6601 ccgggcggca tagccgctac tacctcgata agtacctgtt tgagaccgaa cccagggtct
     6661 tgcgcgggat cgtgaacgag atggccttga tgatccgcaa tcgcatggcc gccggggtcg
     6721 tgtatgatcg gttggcggcg ccggagctgg gtgcggtctc gttgggggcc ggcttgtcgc
     6781 tcgagtcggg catcccgttc ctcatcgtgc ggaagggtga aaagggctac ggcacgtcga
     6841 aggccatcga aggtgcgttt gtgccgaggg accgtgtggc ccttgtggag gacatcgtga
     6901 cctcaggcgg agcggctctg gcggctgtcg agaagttgcg gcaggtgggg ctcgtggtgg
     6961 aagatctgtt ctgcgtggtg gatagggagg aggggggcgc cgaggcggct ctagccgcag
     7021 gattgaccct tcgacctctg ttcacggcat cgggactcgg catcgtcgtc ggctccgccg
     7081 acgagcgatc cgatagaggc gacccgaccg gaggtgagcc cgcatcctaa ggggatgcgc
     7141 agatggggtc gcatgagccc gtaggttggg atattcttgt gcggcaatag cggttttggt
     7201 aaggttgcgc tggatcgaat aactcaaggg aggctacggt gaccaagtct gatttcgtag
     7261 agaaactcgc ggcgaaggct gacctgagca agaaagacgc cgcggctgcg gtggacgctt
     7321 tcgtagacgt cgtctctgaa gctttggcag cgggcgagtc ggtgcagttc accggcttcg
     7381 gaaagttcta cacgcagcag cgcgaagcgc gcgaaggcat caacccgcag acgaaggcga
     7441 agatccagat cgctgctacc accgtcccca agttcagcgc cggtagcggt ctgaagagcg
     7501 ccgttcgtta gtcccgatct cccttgtgtc ttcctgggcc gcccttccgg gggcggccct
     7561 tctggttccg gggggcttga accgaggctg ctctctcgaa cccgatcacg actctcgacc
     7621 tgagggccca tgaagaactt cgccgatcgt ctactggggg ccgtggagga ccgccgttcc
     7681 catgtggtcg tgggcctcga tcccgactac tccttgctgc cgcccgaact ccgtacgctt
     7741 gtgccgtccc aggtggccga cgcggacgct ctggcggagt gcttcgatgt gttccttcgt
     7801 ggcatcctgg gcgcggtcgc cgggatagcg gtggcggtca aaccccaact cgcttacttc
     7861 gaggcgctcg gctccgcggg gtatggattg tacgaacgga tcgtcgcgga ggcgcgcgag
     7921 cagggcttcc tggtgatcgc ggatgcgaaa cgcggcgata tcgggagcac ggcggaggcc
     7981 tacgcccgcg cacacctgga tgtggtcggc gccgacgcgg tcaccgtgaa cccgtggttc
     8041 ggcacggacg gcatcgagcc gttcatcctt cgcacgaagg atgacggccg gggtctgttc
     8101 gtgctggtga agacttcgaa cccggggtct gccgagatcc aggacctcgt gctcgccgca
     8161 ggcggttccc cggtattcga acacgttgcg ggactcgtgg atacctgggg cgtcggcgcc
     8221 gtgggagagt gcggatactc gtcggtgggc gccgtggtcg gggcgaccca ccccgaacag
     8281 gccgcgcggt tgcgcgtggc gcaccccacc acgcccttcc tggtgcccgg ttttggggcg
     8341 caaggcgccg gtccttccca gttgtcaggc atcttcgacg ctggcggcac gggcgcggtg
     8401 gtcaactcct ctcgggcgat cctgtatgcc taccgcgccc ggggcggaga ctggcgggct
     8461 gcggcccgcg acgaggccga ggagatgagg cgcgcgctgt gggccgtggc tcatgggggc
     8521 ggagagtcgt gagcggggct gtcggacggg tcctggcgcc tttggcggcg ctggccttgg
     8581 ttgtccttct ggtttcgctc gtcggtggta gcgatcttct ggcaggcgtc gaccgccccg
     8641 aaggcccgga gacccctgag acctcgcgca ccacgatcat ctacctgacc gagcccgggg
     8701 gcaaccaggc cgatgcgggg gcaggcgccg gcgccggcag cgccggcggg gcttccgtgt
     8761 ctgctcctgg gtccgactcc tccgcgacgg cgggctctga aggcgcgtcg acgggtggtg
     8821 cggccagtgg tgcgacgggc ggtgcggcga gcagcaccac gggttcgcag tccacgggcg
     8881 tttcgaccga tccggcggcg caggagacca cctacacggt ccaggccggc gacactccct
     8941 actcgatcgg cctcgccttc ggcgtgtcga cccaggaact catgcgtgcg aacggcatct
     9001 ccgaccccac gaacctccaa gtgggaacgg tgttggtgat accggcgaag tgatcgtggg
     9061 gggcgcgcgg gtcggtgggt ggggtagtca cggcacgccc agcgccggac cgatcgatca
     9121 ctacgcggcg ggcgttgctc cagagcggcc gcccgcagcc cttcgcacgg ctttcaccgc
     9181 gaagagtccg agggtcagca gggggttccc caagaagctc acgtgacacg ctcccaaatc
     9241 gaggtggcgt cgccgccccg agcgcagaag gtgcggctgg gaatccatcg agatcatccg
     9301 ttcgcgcccg gccgtgcggg tgccgaccca caggaacact cgtgtgggtc ggatctccgc
     9361 tccgcctatg cgtcccgcgg tgacggcgag ggctctctcc tgatggcgca cggcgatctc
     9421 atccacccga tagcgctccg gcagaccttg gcccatcggt cacaacctgc gcaggtggtc
     9481 gtaggtgaag atcaggacca cgtcaaggag cagggttatc aaggcctcga cgaggagctg
     9541 gctcacgacg aacaggggga agaccccgtc ggcggactgg tagtagcgat cgaacagcgc
     9601 gaaggacccg gaccacagca gaagcgcgac cagatagatg gggatcacca tcatccagcc
     9661 gcggcgcagc agcttgatgc tgcgtttggc cgcggcgatc ggagagagac tctcgaacac
     9721 cagggcgtag tcgccgaaga ccagcaggat cgccaagggt aacaccgcga gagagagggc
     9781 ctggccggcg gccgattcga cagccaccaa gtacggcacg atgtagaaca ccgcgtaggt
     9841 gaaggcgtag aactgcgcca gacgcacgaa ctcgccggtc gaccgaggtg agcgcgggta
     9901 ggaggaaccg gcgatcgcgc ggaagtaggg tgccctcaca gcggcggaca acaggcagat
     9961 cgcggccccg gccagaagag cgatgagcgc atggtcgagg ccgccggcga gatcaccgat
    10021 gtgctcttgg aggaaggtcc agcgagtgaa gtcggtctgg gcgaactcgg agaagctcag
    10081 cctgccgccg acggatgcgt agaccgcaaa gaaggctacg acgttgatga cccctaggat
    10141 caaggggtag acgaagagta gctgcttgtg cgtgagtctc gcccacgcgc tgcgcaaggc
    10201 ctggatgatc gcacgacctc cgtggctgtt cggtgggtcc ccgcggtcgc gagtatagca
    10261 ccggccgtgt gcgcttcacc gggccggtct gcccgtcgcg gtcgggcgtt ccgcttgggc
    10321 attggtggcg ggtgaggtcc tccaaggcct gggggagcga gggcccatgc tatactcctg
    10381 cccatggatc ggagaactac gcgccgggtg ggagccaccc ttctactgct cctgtggctc
    10441 ctccttggcg gatccggttt ccccggggct cccggcgcca ccgaagccct gtccgctacc
    10501 accacgaccg ggcagaccgc gcaggttggg cagaccgggt cgacgcagca ccaagtatct
    10561 tccgaggtgc tgcccccggc cgcgacccag gatccgaagg ccgccgcgga ccgcgtcccc
    10621 gctgtggggg ggaagtcggt catcctagtc aacatggatg acggccgggt catctacgag
    10681 aggaacgcgg acgagcagcg ctccatcgcc tcgacgacga agatcatgac cgggatcatc
    10741 gcgctcgaga cgctccccct cgacaagata gtcacggcgt cggccaaggc gaccgacgcg
    10801 ggggagtccg agatctggat ggaaccgggt gagcagctct cggtgggcga cctcatgaaa
    10861 gccctcctcg tgcggagcgc caatgacgcg gctgtggctc tcggggaagc agctgcgggg
    10921 agcctggatg ccttcgtcga gaagatgaac gccaaggcgg cggaactcgg gctcatcaac
    10981 acacacttcg ccaaccctca cgggctcgag gcgaaggagc actactcgtc ggcacgcgac
    11041 ttggccaagc tggcgcggta cgcgatgcag aacgaggagt tccgcaagct tgtggctacc
    11101 gcgaaggtga acatcccgtg gccggggcga ccgtacgacc gcgtgctcaa gaaccgcaac
    11161 gacctcgtcg gcgccgtgca gttcgtcacc ggggtcaaga gcggatacac ggccaaagcc
    11221 gggtattgcc tggtgggatc gggcgcccgc gatggagcgt cgctggtgag cgtcatcctg
    11281 ggcgaggaca cgaaggcggc tgtcgatgcc gacacggtga gcctgttgga gtatgggttc
    11341 acgaagtacc gccaggtgtc gctcatggaa gagggagtgc cggtagccgc gatggatgtg
    11401 ccctaccact tcggagagaa ggtcgtgttg aagaccgagc gcaacctcgt tcgcacggtg
    11461 tacagcgacg acccgatcac caagacggtg gtggtgcaag accgtctggt gctcccagtg
    11521 agccaaggcc aggtgctcgg gaagatcacc ttcgccaccg gcacggccgt ggccggcgag
    11581 gtcgacctgg tgtccgaccg ggcgatcgag gcaccgacgc tgcgcgtgaa actgaggtat
    11641 gtatgggacc ggatgatcaa ctggctgggg aaggctgtgt gagcttcgga tgatcctgac
    11701 agtgacactc aacgccgccg tcgaccgcac gctgacggtg ccgaacttcg aggtgggctt
    11761 tcgccaccgt gccagcgaga cgatcacctt gccgggaggc aagggcatca acgtggcgcg
    11821 ggtggtcaag accctcggcc agccggtcat cgccacgggt tttgccggag ggcgcacggg
    11881 tgatcgcatc atctccgacc tcaaccgcga ggggatcctc tctgacttcg tgcgcatcga
    11941 gggagtgtcc cgcacctcga cagctgtgct cgacccgacc accaaccgga ccacggagat
    12001 caacgagtac gggccggaca tctcgccccg cgagttggaa cttatgctgg agaagatcga
    12061 ctacctgatg aaggccgccg acctggtgat agtggcgggg agcctcccga gcggtctgcc
    12121 cacctcgttc tacggggagc tgataccgcg cgtgcgcgcc gccggcgtgc cggtgctgtt
    12181 cgacacgtgg ggcgaggctc tgcgtctggg catcaaggcg agcccggacg tcgtcttccc
    12241 gaaccaggtc gaggcggaga tggtggtggg ctacgagttc agcagcaatg aggacttcat
    12301 acgggccgcc gagaatctgc gcgacttggg cgcccgctcg gccgtcatca agtcgaagat
    12361 ggggtgtgta gcgaagatca agtacaacgg cgacgtccac acactgatgg gcaaagctcc
    12421 caaggtggag gccgtgtcca ccgtcgggtc gggagactcc atcgtgggtg gctacgccgc
    12481 caagttgctc gaaggggctc ctcctgaaga ctgcctgagg ttcggcctgg cgtgcgccgc
    12541 cgccaacgcg cttcgaccgg gggccggcgt cttcgatccg aacgacgccg cccgactggc
    12601 gcccggtgtg gagatggagg aggtatgagc gggatgacgg gtcgtgacga acccccgcac
    12661 aaggtcctgg tgctcgactt cgggggccag tatgcgcagc tgatcgcgcg cagagtgcgc
    12721 gagtgccgcg tgtattcgga gctgatgcct ttcgacacgc cgctctccga gctcatggcg
    12781 gccgatcctg cgggcatcat cctctccggc gggccgcgaa gcgtcaacga cgccggcgcg
    12841 cccgtgcccg atcccgggat cctcgagctg ggggtgccgg tgctgggcat ctgttacggg
    12901 ctccagctgc tcagcaagct gagcggcggt gtcgtggcac gcaccgaccg ggcggagtac
    12961 ggcggcatgg acatcgaggt ggtcgccgac agcgctctct tcgaggattt gccccgcgat
    13021 caaccttcgt ggatgagtca cggcgacgcg gtgctcgaga cacctccggg attcacgctc
    13081 acagcccgca gccggtcgct gccggtcgcg gcggtagaag atgtgcggcg gcatctctac
    13141 ggcgtgcagt tccaccctga ggtgaggcac acccggcacg gacaggacat tctcaagaac
    13201 ttcctctacc ggatctgcga catcgctccc acgtggacgc cggtgtcgat catcgaagac
    13261 gcggtggccc gtatccgcga gcaagtgggt gacggtcgca tcatttgcgg actctcgggc
    13321 ggggtggact cggctgtggc cgccctcctc acctacaagg cggtgggtga ccaactcacc
    13381 tgcatcttcg tcgatcacgg gctgatgcgt aaggacgaag gcgaacaggt ggagagcgcg
    13441 ttcggatatc acttccacgt gccgctggtg cacgtgcgcg cgcaggagcg cttcatcacc
    13501 aaactggcgg gcgtccagga ccccgagacc aaacgcaaga tcatcgggga ggagttcatc
    13561 cgcacgttcg aggcggaggc gcgacgcctg ggcgacgtcc gcttcctggt gcaggggaca
    13621 ctctactccg acgtcatcga gtcggggacg cgtgacgccg ccaagatcaa gtcgcaccac
    13681 aacgtgggcg gcctgcccga ggacatggac ttcgatctgg tggagccact gcggatgctc
    13741 ttcaaggacg aagtgcgcgt cgtgggcgag gagttggggc tgcccgactc catggtgtgg
    13801 cgccagccct tcccgggtcc ggggctcgcc atacgcatca tcggtgacgt caccgaagag
    13861 cgcctccaca tccttcgtga ggccgacgcc atcatcctcg acgaggtgcg gaaggcgggt
    13921 ctctataggg agctgtggca gtcgttcgcg gtgctccccg ccatccgcag tgtgggcgtc
    13981 atgggcgacg aacgcaccta tgcctaccct atcgtcatca gggcggtcac ttccgaagac
    14041 gcgatgacgg ccgattgggc ccgtctgccc tacgaactgt tggagcgcat ctccagccgt
    14101 gtgatcaacg aggtgcccgg tgtgaaccgg gtggccctcg acgtctcaag caaacctccc
    14161 tccaccatcg agtgggagta aggagcacca tgaccgacga aacgatgcga ccggaggatc
    14221 atgacgaggg cgaagacgcg cccgaggtca tcatggtcac cggccccgag ggtgaagacg
    14281 cgcgtctcga gccgaatgag gatctcgacg tcgacctgga agcgcgtgga atcgctgatc
    14341 ttccggccga cgagaccacc gatccggagt ctcaggaggg tccacgtgct ctcggcgtcc
    14401 ccgtgcgtac gcagccgagg gagcggagca ccgacgacaa gttggagctg atccaccggc
    14461 acactcttct ggccgcggcc accgcctcgg gtatgaaggc cgaggatatc cgcctcctgg
    14521 acgttcacga actggtgagc tacacggact tcctgctcat ctgcaccggc cgctcggctc
    14581 ggcagacggg acggatagcc gaggagatcg ccctcaagtt aaagcaggag ttgggcctca
    14641 tccccagcag cgtggagggc gagcgccccg gcgagtgggt gctgctggac tatctggact
    14701 tcatcgtgca cgtgttcacg cccgaggccc acgacttcta ccggctcgac gttctgtgga
    14761 agcaggcgcc gacggagatc atcgagtaga aggggcggaa ccggactcgg cggcgccccg
    14821 gcgtggcggc gcctccgacc tcggccggcc ccgtgggcca ggcaggttgc gcggtgccgg
    14881 cgccccgggc gtggcgtggt ggcagatggt gcccaccacg ccccgtgcgg ttgttgcacg
    14941 gctcccggac agtcggtata ctccaccccc gcttgacgtg ggggtatagc tcagttggga
    15001 gagcgcgacg ctggcagcgt tgaggccagg ggttcgagtc cccttacctc caccataagt
    15061 ctcttactca aaccatgtgc caaggaaaac accgctcggc acctggcagg acggtctgag
    15121 gcgttcgatt taggaacgag cccactcaat tgagtgggct cgttcgcgtt ctcggagacc
    15181 tccgattgtt cgtggcgaca ggcagagcgt agtcccggtc cgaacaacat gtcgaacggg
    15241 ggcgcgagtt cgggttctag actggcgtcc gagttgacca ggattcgctt gaagaaggcc
    15301 tggttgaact gtttcttgat gtgagcgggt gccgtcttgt acgccagagc gcagttcacg
    15361 gtgagatcga gcgcggcctg gaggttgtgc tcgatgacat cgaagtgaac gtttgaggcg
    15421 tcgagcttgc gtgcggtttc gagcagtccg gcagcgatac gatcctgctc ccgtttgagc
    15481 agttcgagcg ggatggcccc ctcgtagtgg gcctgcatga gcttgtcgcg ctcgcgctct
    15541 aacttctcgc gctgtcgtcg tacatcgcgc agctcggctt ccgaatcggc ccgcaccatc
    15601 ttcagctcct cgacgagcaa ggtctcgact ttgaggcgaa aatccgggtc gagcgcgatg
    15661 ttcgcataat gttcctcgat gcgtgcttcc acctcgtcga tgagaactgc tttctgacga
    15721 cagtcggtgc gcttactgtg ccgccccgag cagatgaagt aggggtagta gacgcccgag
    15781 cgggacttgg atatctgcac gagcagcctg gacccgcaga tcccgcagta gacggtgccc
    15841 ttcaggaaat gaggatggtc tctggtacgt tctccgttga cgtgggcggc gagcatgtct
    15901 tggacggcct gccacgtctg ctcatcgatc agcggctcat gtttgccggg gtgcagtgca
    15961 ccttggaatg ggacacaacc cttgtagtac gggttgacca gcagcttgtt gagtttggcc
    16021 gcgttgacgg gcacggatgg gacgcgcgga gtggcaaggg tagccagccc gcggtcggcg
    16081 aggtgctcgg ccagcgcggc gatagtccag tcaccggtgg catagagtga gaagcctagg
    16141 cgtacgagtg gtgcacgctc ttcgtccacc gtgacggtgc gcatctcgcg gccttcgctg
    16201 tccgtggtgc gaatgttgcg gtagcccagc ggcgctcgac tgacggtgcc gccgccacgg
    16261 accttctggg tcattccctt gacgacctcg ttggcgaggt ttcgggagta gaactcggcg
    16321 atggatgaca tgatgccgtg cagcagcatg ccggacggag tctggt
//
LOCUS       DASRMJ010000330         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_34779, whole genome shotgun sequence.
ACCESSION   DASRMJ010000330 DASRMJ010000000
VERSION     DASRMJ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34779"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFD74_09180"
     CDS             <1..1017
                     /locus_tag="VFD74_09180"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZK49753.1"
                     /translation="EEATRPPPLAGAQAVEVARQGWGARRFLSPASLRNALAIDAALG
                     CSTNTVLHLLAIANEAGIELPIEAFNEVSAATPHIVKLSPSGSDHMEDLERAGGIMAV
                     ARRLAEGGLVDGSVPTVSGGSLAHQYDAVSIRDADVIRPLDQAYGATGGLAVLFGNLA
                     PGGAVVKEAAVLPGMLQHRGPAMVFDDEASVVAAIEADEIAAGCVVVIRYVGPKGGPG
                     MPEMLTPTSALAGAGLDDRVALITDGRFSGASRGASIGHVAPEAVDGGPIALVREGDI
                     ISIDIPARQVTLEVSDSDLAKRRVAWSRPERQLSGVLARYVTQVSGADRGAVLTPGGR
                     DAGS"
     gene            1137..2834
                     /gene="ilvB"
                     /locus_tag="VFD74_09185"
     CDS             1137..2834
                     /gene="ilvB"
                     /locus_tag="VFD74_09185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZK49754.1"
                     /translation="MNSAIAILESLKREGVDVVFGYPGGSVIPLYDALYDFDLDHILT
                     RHEQGAAHAADGYARASGKVGVCIATSGPGATNLTTGIANAYLDSVPLVAITGQVRRP
                     LIGTDAFQEADVTGITLPIVKHSYLVKRAEDLPAIIHEAFYIAGTGRPGPVVVDIPVD
                     LTTMDLDFDFEAAQKIDLPGYKPTIKGHPKQIKAAAQAIAEAQRPVIFAGGGVISANA
                     SDELRELAVYAHIPVTTSLTGIGSFPEDHEELSLGMLGMHGTRYANYAVTGCDLILGV
                     GVRFDDRVTGKISTFAPGAKVVHIDIDPAEISKNVAVDIPIVGDAKRVLADLLTQLKK
                     HELSPERHLPWMRQVADWKRAHPMVFKQEEDGEIMPEYVVHMISEITKGEAIICTEVG
                     QNQMWAAQYSRIKHPRHFISSGGLGTMGFGFPAAIGAKVARPDKTVVDIAGDGSIQMN
                     IQELGTAMNYRIPVKVCILNNQYLGMVRQWQDLFWNKRYSATCMACQPDFVKLAEAYG
                     AQGFRATKADEVEEVLRVAFASDEPALIDFRIKRESNVWPMVPAGGSIGEMMGEGDR"
     gene            2831..3316
                     /gene="ilvN"
                     /locus_tag="VFD74_09190"
     CDS             2831..3316
                     /gene="ilvN"
                     /locus_tag="VFD74_09190"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZK49755.1"
                     /translation="MKHTLSVLVENKPGVLTRIAGLFARRGFNIDSLAVGETEDPRIS
                     RMTITVDAQDQPLEQVTKQLHKLIDVIKITDLDPATSLDRELVMVKVKADAATRSEIM
                     QVVEIFRAKIIDVSRDALVIEVTGTREKLAAIMSLLRPFGIKELVRTGRIAMDRGREL
                     T"
     gene            3432..4439
                     /gene="ilvC"
                     /locus_tag="VFD74_09195"
                     /pseudo
     CDS             3432..4439
                     /gene="ilvC"
                     /locus_tag="VFD74_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784702.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
BASE COUNT          835 a         1556 c         1530 g          724 t
ORIGIN      
        1 gaggaagcga cgcgcccccc acccctggcg ggcgcccagg ccgtcgaggt cgcccgccag
       61 gggtgggggg cgcgtcgctt cctctcgccc gcttccttgc gcaatgcgct cgccatcgac
      121 gcggcgctgg gttgctccac caacacagtc ctccacctgc tggccatcgc caacgaggcc
      181 ggcatcgagt tgcccatcga ggccttcaac gaggtctcgg ccgcgactcc gcacatcgtg
      241 aagctctcgc cctcgggttc cgaccacatg gaagacctcg agcgcgccgg cggcatcatg
      301 gccgtcgctc ggcggctcgc ggaaggcggc ttggtcgacg gttccgtgcc cacggtgtcg
      361 ggcggctcgt tggcccacca atacgacgcc gtctcgatcc gcgatgccga cgtcatccgg
      421 ccgctggacc aggcctacgg ggccacgggc ggcttggccg tgctcttcgg gaacctggct
      481 ccgggcggcg ccgtggtcaa ggaggcagcg gtgcttcccg gtatgctgca acaccggggc
      541 ccggcgatgg tcttcgacga cgaagcgtcg gtggtcgccg ccatcgaggc cgacgagatc
      601 gcggccgggt gcgtcgtggt catccgctac gtcggcccca aaggggggcc gggcatgccc
      661 gagatgctca ccccaacgtc ggccttggcc ggcgcgggtc tcgacgatcg cgtggcgctg
      721 atcacagacg ggcgcttcag cggcgccagc cgcggagcca gcatcggtca cgtggcgccc
      781 gaagccgtcg acggagggcc catagccctc gtccgggaag gcgacatcat cagtatcgat
      841 atccctgcac gtcaggtcac gttggaggtc tcggactccg acttggcgaa gaggagagtg
      901 gcctggtcac gtccggagcg acagctcagc ggcgtgctgg cccgctacgt aacccaggtg
      961 tcaggggccg accgcggggc ggtacttacc cccggcggtc gcgacgccgg cagctaggag
     1021 gggcactccg gcaggagtcc acttcgttgg tccggtcggc gtcatacgct ccggcgcccc
     1081 tgcagaggat cacctccgtc gatactggaa tcccgagaga agaggcttgt tccgcgatga
     1141 actcagcgat agcgatactg gagtccctca agcgtgaggg cgtcgacgtg gtcttcggct
     1201 accccggggg gagcgtgatc ccgctctatg acgccctcta cgacttcgac ctcgatcaca
     1261 tactcacgcg acacgaacag ggtgcggcgc acgcggccga cggctacgcc cgcgcctcgg
     1321 gcaaggtggg cgtctgcatc gccacctccg gccccggcgc cacgaacctc accaccggca
     1381 tcgccaacgc ctacctcgac agcgtgcctc tggtggcgat caccggccag gtgcgccggc
     1441 ctctcatcgg caccgatgcc ttccaggagg ccgatgtcac cggcatcacc ctgcccatcg
     1501 tgaagcacag ctacctcgtc aagcgcgcgg aagaccttcc cgcgatcatt cacgaggcgt
     1561 tctacatcgc cggcaccggt cgccccggcc cggtggtggt ggatattccg gtggacctca
     1621 ccaccatgga cctcgacttc gatttcgaag cagcgcagaa gatcgatctg ccgggataca
     1681 agcccaccat caagggtcac cccaagcaga tcaaggcggc ggcgcaggct atcgccgaag
     1741 cgcagcgccc ggtcatcttt gccggcggcg gcgtgatctc ggccaacgcc tcggacgaac
     1801 tgcgcgagct cgccgtctac gcccacatcc ccgtcaccac gagcctcacc ggaatcggat
     1861 ccttccccga ggaccatgag gagctctctc tgggcatgct gggcatgcac ggcacccgct
     1921 acgcgaacta cgccgtcaca ggctgcgacc tcatcctcgg ggtcggcgtc cgcttcgacg
     1981 accgagtcac cggcaagatc tcgacgttcg cgcccggagc caaggtcgtg catattgata
     2041 tcgatccggc cgagatctcc aagaacgtgg ccgtggacat ccccatcgtc ggcgatgcca
     2101 agcgggtgct cgccgatctg ctgacgcagt tgaagaagca tgaactctcg cccgagcggc
     2161 acctcccctg gatgcgtcag gtggccgatt ggaagagggc ccatcccatg gtcttcaagc
     2221 aggaagaaga cggcgagatc atgcccgagt acgtggtgca catgatctcg gagatcacca
     2281 agggcgaggc gatcatctgc accgaggtgg ggcagaacca gatgtgggcc gcgcagtact
     2341 ctcgaatcaa gcatccgcgc cacttcatct cgtccggcgg tctgggcacg atgggcttcg
     2401 gcttcccggc cgcgatcggc gccaaggtgg ccaggcccga caagaccgtt gtcgatatcg
     2461 ccggcgacgg ctcgatacag atgaacatcc aggagctggg cacggccatg aactaccgca
     2521 tcccggtcaa ggtgtgcatc ctcaacaacc agtacctggg catggtgcgg cagtggcagg
     2581 acctgttctg gaacaagcgc tacagcgcca cctgcatggc ctgccagccc gacttcgtga
     2641 agttggccga ggcctacgga gctcaggggt tccgcgccac gaaggcggat gaggtcgagg
     2701 aggtgcttcg tgtggccttc gcctccgacg aaccggctct gatcgacttc cgcatcaagc
     2761 gcgagagcaa cgtgtggccc atggtgcccg cgggcggctc gataggcgag atgatggggg
     2821 agggggaccg atgaagcaca cgctctccgt cctggtggag aacaagccgg gggtgctcac
     2881 ccggatcgcc ggactgttcg cccgacgggg cttcaacata gacagcctgg ctgtcggcga
     2941 gaccgaggat ccgaggatct cgcgcatgac cataaccgtc gacgcccagg accagccgct
     3001 ggagcaggtc accaagcagc ttcacaagct gatcgacgtc atcaagatca ccgacctcga
     3061 cccggccacg agcctcgatc gcgagctggt catggtcaag gtcaaggccg acgctgccac
     3121 gcgttcggag atcatgcagg tggtcgagat attccgggcc aagatcatcg acgtctccag
     3181 ggacgcgctg gtgatagaag tcacgggcac ccgggagaag ctggccgcca tcatgagcct
     3241 tttgcggccg ttcggtatca aggagttggt gcgcacgggt cgcatcgcga tggacagggg
     3301 tagggaactc acgtagggag ggacggcggc gccgggcggc aaagcccgcc cgcgtcgccg
     3361 ctcgcttccg gcgtcagagg cagagtcagc agacacacgt cttcaacggg tcttcaacag
     3421 agacagtcag ggaggaacag atgtactacg acaaggacgc cgacctcgag cttctgcaag
     3481 ggaagacggt ggccgtgatc gggtacggga gccaaggtca cgctcacgcc cgcaacctca
     3541 aggacagcgg catctccgtg gtggtgggcc ttcgtgaggg ctcgaagagc gtgggtcgcg
     3601 cccgcgaagc cggcctggag gtgctctcgg tggccgaagc cgccgcccgc ggcgacgtcg
     3661 tcatgatcct tgctcccgac caggaccaga agtccgtcta cgagacctcg gtgcgcgaca
     3721 acctcaaaga cggcgatatg ctcatgttcg cccacggctt caacatccat ttcgggcaga
     3781 tcgttcctcc ggccggtgtc gacgtgacca tggtcgcccc caagggtccc ggtcatctgg
     3841 tgcggcgcac ctacaccgag ggtggcggcg tgcccggcct cgtggccgtc catcaagacg
     3901 ccaccggcca agccaaggag aaggccctgg cctacgccaa aggcatcggc gccacccgcg
     3961 ccggcgtcat cgagaccacc ttccaggaag agaccgagac cgacctgttc ggcgagcagg
     4021 ccgtgctctg cggcggctgc agcgagctgg tgcgcgccgg tttcgagacc ctggtcgagg
     4081 ccggctacca gccggagatc gcctacttcg agtgcctgca cgaactcaag ctgatcgtcg
     4141 acctcatgta cgagcacggc atcagcggca tgcgctactc gatctccgag acggccgaat
     4201 acggcgacgt cacccggggt ccacgtatca tcacgcagga gacgcgcgcc gagatgcgcc
     4261 gcatcctggc cgagatccag tcgggcgagt tcgcgcgcga gtggatcctt gagaaccaag
     4321 ccggtcgccc ggtgtacacc gcgctcaccc gcaaaggtgc cgacagcttg atcgagcagg
     4381 tgggccaaaa gcttcggggc atgatgagct ggatctcgag cggcaagacg ctcgagtagc
     4441 gcattcgcgc ccggccggcg caaccgacgc cggtcgcgcc ggcgggcgcc ggcgacgccg
     4501 gtcgcagccg cgggtcgagg atgacatggt ctcacgtgtg gggcggggcg ccgagctccg
     4561 ccccactggc ctgcgtacag ggccggcact gtacagccca tagaccgcat cgtatgcttg
     4621 tgttcgtggt tcaaggggat actca
//
LOCUS       DASRMJ010000331         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_503269, whole genome shotgun sequence.
ACCESSION   DASRMJ010000331 DASRMJ010000000
VERSION     DASRMJ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503269"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFD74_09200"
     CDS             <1..646
                     /locus_tag="VFD74_09200"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK49756.1"
                     /translation="FKEVVDGFFSDRRVPFISLAKSDAVVVLLQAGSSEREHFRSLGW
                     ELHAHLAAALPDLTVTIGFGESYDEPNQIPRAFTQAREAKSARNTPGSLDRVRLFSDL
                     GPRISSLESQTADRLEYFVESTLGRLAAHDAERGGRLLETLSVYLGADRNVGRAAEML
                     FVHPNTLRNRLHRVEDLLGVSLDSTDALVDVTLALQSMRILESMRGRPGSAGPV"
     gene            complement(693..2429)
                     /locus_tag="VFD74_09205"
     CDS             complement(693..2429)
                     /locus_tag="VFD74_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZK49757.1"
                     /translation="MTRTLFIDLAAGKVEVEELEISGLSGLGGKALAIELLERYLDPT
                     IDPLAPANVVVFTPSPLAAYGFSGSDRFGAFTKSPMTGAFLESYAGGTMARTLRETGW
                     DAVVLRGASGAPVRVHIDADGATITSAAGLWGKDVFETEDALRASLAKRSSTMIIGPA
                     GESLSVLAAVILEKDHALGRGGLGAVLGSKKVKAITVTSPGPVRLEAGETFEAVRRAV
                     SKLATESPAAKAYRSFGTPMMVAVLNENGAFPTGYWESGRAAHRAGLEAENFPTWAKV
                     ETGTCPPCPVRCRRHLTILEGPHAGREVHGPEYESLYAFGGLCMVEHARDVLFLHEEC
                     NRLGMDTMSGGNLVSLAMRAAATGRLDDAPAPGDAEAISDLLARMAYRDSELGDTLSR
                     GIRGAATALGMEDEAIHVKGMEPAGYDPRSMRGMALAYATSPRGACHLRSTFYKPILA
                     GMIKDKNAAETIEVFVDFEDRMFLYDCLIMCRFYRDFLTWDDLAAMTSQLAGASVDVP
                     ALKRLAGDLLTRVRRLNFAMGLTAADDTLPKRFFTEGMDDKPPIGERELGDWLDAYFG
                     ARGWPEGVPPAA"
     gene            complement(2426..>4132)
                     /locus_tag="VFD74_09210"
     CDS             complement(2426..>4132)
                     /locus_tag="VFD74_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZK49758.1"
                     /translation="VGGKGLGAHYLSTELPAGVDPLGPENILIFMTGPVSGVFPGTCR
                     HAVVTKSPATGGFLDTYAGGFFAWELRKAGLLGIIIKGAAAKLSYLEVTADGATIRDA
                     TQFAGMSIADVDDDPLLKDYRVAAIGVAGENGVLMASIGNNAGSTKNGRSGFNGRGGS
                     GAVMGSKNLKAIAVKGGKAPATSDAAKGVRKDLSKKIMAEGSASSWLADAGTSAIVDW
                     TNGVNVLPTRNWHSGSFEDAAALVGHEAVIANLVAREGCYNCPVNCGTHVKAKEGAFP
                     GVEADKLEYETIGLGATNTGNGDFSSIAKFARQCDELGIDTISCGAAVAFAMDAAEQG
                     FIDHPIRFGDSEGQAALVEDIAHRRGIGAELARGIRLAAKAWGVDDSKVPVFEIKGLE
                     FPAYDPRGSIGMALAYATADRGACHMRSWPIAADALSEDENADPFGPEGKAEFIAGEQ
                     DENCSEWSLVGCDFIVYDADDAATMLEAVGIEKSADEYRRMGTRIWNIVRLFNLRQGW
                     TAEDDYVPQAIHNPLDDSGKALTAEAFALMKKEYYEVRGWDEAGRPTQATIDELGLGE
                     YV"
BASE COUNT          623 a         1390 c         1402 g          717 t
ORIGIN      
        1 gttcaaggag gtcgtggacg gtttcttctc agataggagg gtaccgttca tctctctggc
       61 caagagcgac gccgtggtcg tgctgttgca ggcgggatcc tccgaacgcg agcatttccg
      121 ctccctgggg tgggagcttc atgcgcatct cgccgccgcc ctgcctgatc tcaccgtgac
      181 gatcggattc ggcgagagct acgacgagcc caaccagatc ccgcgggcgt tcactcaggc
      241 acgggaggcg aagtcggccc gcaacactcc gggctcactc gaccgggtgc ggctcttctc
      301 ggatctcggc ccgcggatca gctcgctcga gagccagacc gccgatcgcc tggagtactt
      361 cgtggagtcg accctcgggc ggctcgccgc ccacgacgcc gaacggggtg gcaggctcct
      421 cgagacgctc agcgtctacc ttggggccga ccggaacgtg gggcgcgctg ctgagatgct
      481 cttcgtgcac ccgaacacgc ttcggaaccg actgcacagg gtggaggatc tgctgggcgt
      541 ctccctcgac tccaccgacg ccctggtgga cgtgaccttg gctctccaat ccatgcggat
      601 cctggagtcg atgcggggcc ggccgggatc ggccggcccc gtgtagctcc aagccgaagt
      661 tctcgcccgc ccccgttcga aggttctgcg gctcaggccg cgggcggcac gccctcgggc
      721 cagccgcgcg ccccgaagta ggcgtcgagc caatcgccca actcccgctc gccgatgggc
      781 ggcttgtcgt ccatgccttc tgtgaagaag cgcttgggca gggtgtcgtc ggcggcggtg
      841 aggcccatgg cgaagttgag tctgcgaacc cgggtgagca ggtcgccggc cagccgcttg
      901 agtgccggca cgtcaacgga cgcaccggcg agctgcgacg tcatcgcggc cagatcgtcc
      961 cacgtgagga agtcgcggta gaagcggcac atgatcaggc agtcgtagag gaacatgcgg
     1021 tcctcgaagt ccacgaacac ctcgatcgtc tccgcggcgt tcttgtcctt gatcatgccc
     1081 gcgaggatgg gcttgtagaa ggtggagcgc aagtggcacg cgccgcgagg gctggtggcg
     1141 taggctaggg ccataccccg catcgagcga gggtcgtatc cggccggctc catgcccttc
     1201 acgtggatgg cttcatcttc catgcccagg gcagtcgccg cgccgcgtat gccgcgcgac
     1261 agcgtgtcgc ccaactccga gtcgcggtag gccatccgcg cgaggaggtc ggagatcgcc
     1321 tccgcgtcgc cgggggcggg cgcgtcgtcg agtcggccgg tcgctgcggc tctcatggcc
     1381 agcgagacca ggttgccgcc actcatggta tccatgccga gtcggttgca ctcctcatgg
     1441 aggaagagca cgtctcgcgc gtgttccacc atgcagagtc ctccgaaggc gtacagcgac
     1501 tcatactcag gaccgtggac ctcgcgtccc gcgtgggggc cttccaggat cgtgaggtgg
     1561 cgccggcagc gcacggggca cggcgggcag gtgcccgtct caaccttggc ccacgtgggg
     1621 aagttctcgg cctccaggcc ggcccggtgc gcggcccggc ctgattccca gtagcccgtg
     1681 gggaaggctc cgttctcgtt gagcaccgcc accatcatcg gagtgccgaa cgagcgatag
     1741 gccttagccg ccggtgattc cgtcgccagc ttcgagacag cccgtcgcac cgcctcaaag
     1801 gtctcgcctg cctccagccg cacgggtcct ggggaggtga cggtgatcgc cttcaccttc
     1861 ttgcttccga ggacggcgcc cagcccgccg cgtcccagcg cgtgatcctt ctccaagatg
     1921 acggcggcca gaaccgagag actctctccg gctgggccta tgatcatggt cgaggagcgt
     1981 ttcgcgaggg aagcccgcaa cgcgtcttcg gtctcgaaca catccttgcc ccagagtccg
     2041 gcggccgacg tgatcgtggc tccatccgcg tcgatgtgca cgcggaccgg ggcgccggag
     2101 gcgccccgca acacgaccgc gtcccagccg gtctcccgaa gggtccgtgc catggtgccg
     2161 cctgcgtacg actcgaggaa cgcgccggtc atcgggctct tggtgaaggc gccgaaccgg
     2221 tccgagcccg agaagccgta tgcggcgagc gggctggggg tgaacaccac cacgttggcc
     2281 ggtgccagag ggtcgatcgt cggatcgaga taccgctcaa gcagttcgat ggccaacgcc
     2341 ttgccgccca gtcccgagag gccggagatc tccaactcct cgacctcgac cttgccggcg
     2401 gcgaggtcga tgaacagggt gcgcgtcata cgtactctcc cagccccaat tcgtcgatgg
     2461 tcgcctgcgt gggtcgcccg gcctcatccc agccgcgcac ttcgtagtac tccttcttca
     2521 tcagagcgaa agcctcggcg gtcagggcct tgcccgaatc gtcgaggggg ttgtggatgg
     2581 cctgtgggac atagtcatcc tcggccgtcc agccctggcg caagttgaag aggcgcacga
     2641 tgttccagat gcgcgtgccc atgcggcggt actcgtcggc gctcttctcg atccccacgg
     2701 cttccagcat cgtcgctgca tcgtcggcgt cgtagacgat gaagtcgcag cccaccaggc
     2761 tccattcgga gcagttctcg tcctgctctc ccgcgatgaa ctcggccttc ccttcggggc
     2821 cgaagggatc ggcgttctcg tcttcggaga gggcatcggc ggctatgggc caactgcgca
     2881 tgtggcaggc gccgcggtcg gccgtggcgt aggcgagggc catcccgatc gagccgcgcg
     2941 ggtcgtaggc tgggaactcc aagcccttga tctcgaagac ggggactttg ctgtcgtcca
     3001 cgccccaggc tttcgcggcc aagcggatgc cccgggcgag ttcggcgccg atgccccggc
     3061 ggtgagcgat gtcctccacc aacgcggcct gtccctcgga gtcaccgaag cggatggggt
     3121 ggtcgatgaa tccctgctcg gcggcgtcca tggcgaaagc cacggcggcg ccgcaggaga
     3181 tggtgtcgat gcccaactcg tcgcactgcc gggcgaactt ggcgatgctg gagaagtcgc
     3241 cgttccccgt gttggtggcg ccgagcccga tggtctcgta ctcgagcttg tcggcttcga
     3301 cccccgggaa cgcgccttcc ttggccttca cgtgcgtgcc gcagttgacc ggacagttgt
     3361 agcagccttc gcgcgccacc agattggcga tgacggcctc gtggcccacg agtgcggccg
     3421 cgtcctcgaa ggatcccgag tgccagttgc gcgtgggcag cacgttcacg ccgttcgtcc
     3481 agtcgacgat ggcggaggtc ccggcgtcgg cgagccagga ggaggcggaa ccctcggcca
     3541 tgatcttctt ggagaggtcc ttgcgcacac ccttggccgc gtcgctggtc gcgggggcct
     3601 tgccgccttt gactgcgatg gccttgaggt tcttggagcc catcaccgcg cccgagcccc
     3661 cgcggccgtt gaagccggag cgcccgttct tggtggagcc cgcgttgttg ccgatggacg
     3721 ccatgagcac cccgttctca cccgccacgc cgatcgcggc cacgcggtag tccttgagga
     3781 gcgggtcatc gtccacgtcg gcgatgctca tgccggcgaa ctgggtggcg tcgcggatgg
     3841 tcgccccgtc ggcggtcacc tcgaggtatg agagcttcgc cgccgcgccc ttgatgatga
     3901 tgccgagcag tccggccttt cgcagctccc aggcgaagaa gccgccggca taggtgtcga
     3961 ggaagccgcc cgtggccggg ctcttggtca cgaccgcgtg gcgacaggtg cccgggaaga
     4021 cgcccgaaac ggggccggtc atgaagatca ggatgttctc gggcccgagc gggtcgactc
     4081 ccgcaggcaa ctcggtggag aggtagtgcg cacccaaccc tttgccgccg ac
//
LOCUS       DASRMJ010000332        10838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_280766, whole genome shotgun sequence.
ACCESSION   DASRMJ010000332 DASRMJ010000000
VERSION     DASRMJ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10838
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280766"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFD74_09215"
     CDS             <1..802
                     /locus_tag="VFD74_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK49759.1"
                     /translation="PYFVEYGSYVDNHGGVLRLVDTNPDFSLDLDRISAAITPKTRAV
                     ILNSPNNPTGRIYPEETVCELGRLLERKGAETGRIIFLIADEPYRKIVYGDTAVPSLM
                     AAYKNTILATSYSKDLSLSGERIGFVAAGSGIRDIQKLMGGLVLANRILGFVNAPALM
                     QRAVARLQGVHVDLTFYKQNRDLLLGALRGSGFTVPEPDGAFYLFPKSPIEDDVAFAQ
                     ELQERLVLVVPGTGFGGPGHFRIAYCVSRQTVEGAIPIFEEIGHKYFG"
     gene            complement(968..1459)
                     /locus_tag="VFD74_09220"
     CDS             complement(968..1459)
                     /locus_tag="VFD74_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK49760.1"
                     /translation="MIDEELQARLDEQAAAGESRAKALELLASQDVGFLAVGDNDGPY
                     VVPVSFVYDGEDIYLHGGEGKKSRALETDPRACLAVMSGSDLIKGDTPCGDNVDSRTA
                     LVFGAVTRLDSPLQKDAALRAVIAKYHPEAVTDPLDPRTIARTLVYRMEIRAVSYREL
                     PGE"
     gene            complement(1526..2437)
                     /locus_tag="VFD74_09225"
     CDS             complement(1526..2437)
                     /locus_tag="VFD74_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HZK49761.1"
                     /translation="MKHPSTRSIAVIIGAALVVAGSALALAAVYVADPAPLAAPSGVA
                     IDLATNHPATSHPTPADPSAESPSRLAATPQEPPWRSKQREVERSLLRAEGHTDSVGA
                     VALIAFDPALQPTAAPGARSAASSTPTRTVLDGGATPLRGVWAGTAAELSRYLLVSVP
                     SPRFTVPASTLAGYYVHLAAEVGLRADILWAQMLHETGFGSYTGSVRPEQNNFAGIGA
                     TGNGAPGLAFPTAEAGVQAHVAHMVAYVFLADQAAWTNSSTDPRYDSVSPRGAARTLS
                     DLDGRWAVPGVGYSVAIERHVRAINAR"
     gene            2635..3816
                     /locus_tag="VFD74_09230"
     CDS             2635..3816
                     /locus_tag="VFD74_09230"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HZK49762.1"
                     /translation="MKYVVLITDGASGWPVETFAGRTTLEAAEMPNLDRMARTGVVGL
                     AATVPDGMEPSSAVACMSVMGYDPSLFYAGRGPIEALALSIDLEPGQVALRCNLVCVD
                     DGAMRSYSAGNISSSESHVLMAAIQSELGDRRVRFHPGVGFRHILTVREGRDLLATRC
                     VPPHDLADQQIGGHLPDGPAADLLLDLMERSKPILAAHPVNRGRKDRGELPATQIWPF
                     WPGVRPAAMPSFREKYGLRAALTSPVDLLRGIANQLGIDVLEIAGVTDGPDNDYGAQM
                     KGALKALGDHDLVIVHVEAPDAAGHDGDAEGKRTALERIDVEMASQLWEREGGLRVLV
                     MPDHPTPLALRTHAAEPVPFIIWGEGSTGTPAPSFTESAAAGTGLLVPAGHELMAMLL
                     G"
     gene            complement(3813..3977)
                     /locus_tag="VFD74_09235"
     CDS             complement(3813..3977)
                     /locus_tag="VFD74_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZK49763.1"
                     /translation="MDKWECMLCGYVYDPAVGDASQGIAPGTAFEDLPDDWECPDCGA
                     SKEDFEKMEN"
     gene            4280..5611
                     /locus_tag="VFD74_09240"
     CDS             4280..5611
                     /locus_tag="VFD74_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HZK49764.1"
                     /translation="MPEELGEALSIWLVLPFAGLLLSIAILPLTAEHWFEKNRNRALV
                     AGLFGVPVVVYLLARFGAEGLAEVLSTGEEYISFIVLLTALFTISGGIYLTGNLLGTP
                     LTNVAFLLVGAVLANFIGTTGAAMVLIRPLLRANSERHNQKHVVIFFIFVVANIGGLL
                     TPLGDPPLFLGFLRGIDFFWTMRLLPQWALAVGLTVAIFFILDYRAYKSEKPKWIRED
                     IADYVPMRIAGKTNLLFLAGVIAAVLLSKQLAQVGESIHFPFLRELIMGAMILLSLKV
                     GPSGPRKANHFSWAPIQEVAIIFAGIFAAMIPALAILKARGGELGFTEPWHFFWATGV
                     LSSFLDNAPTYLTFTSTAQGFLGINEVPGLMGSGIIESLGRAPAAFLAAISCGAVFMG
                     ANSYIGNAPNFMVKSIAERSGQKMPSFFGYMGYSFVVLVPVFILVTLVFFR"
     gene            5747..7819
                     /locus_tag="VFD74_09245"
     CDS             5747..7819
                     /locus_tag="VFD74_09245"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZK49765.1"
                     /translation="MSLETWAVLAAAAAVLGLIYAAYTASRLVKAEVGTEKMRTIMGL
                     IQSGAMAFLKREYSVLIWFVGFVAIAIAVVGAIWDVGLGWQTAIAYLCGSFLSASAGY
                     LGMMIATRANARTANAARTSLAAALDVAFKGGSVMGMIVGGLGLLGVSVIWLIFREPQ
                     YVNGFALGASSIALFARVGGGIYTKAADVGSDLVGKIEAGIPEDDPRNPGVIADNVGD
                     NVGDVAGMGADLYESFVGSIIATMALGVVLANNATTSKFVILPLIIAGAGILASIVGT
                     LFVKAKEGARLHTALNLGTYVATALTIAAMFVTIGWFLPGDGESWTTYMGFFWGSAGG
                     LLAGVLIGKVTEYYTSDHYKPVKGIAEASQTGAATNIIRGLGVGMESVVVPVLLVVGA
                     IIVAYYLGDRVPLKDSGVYGVALAALGMLSTTAMTISVDAYGPIADNAGGIAEMAELP
                     KEVRARTDQLDSLGNTTAAIAKGFAIGSAALTALALFTAFAETYKTSGGGELTLDLLK
                     GPAVIIGLFLGAALPFLFSALTMNAVGRAAASIIDEVRRQFREIPGLMEGTAVPDSAT
                     CVSIVTGSALKEMILPGVLAVVAPLVVGLISPVALGGLLAGALVTGFLLAIMMANAGG
                     AWDNAKKFIEGGEYGGKGSAPHAAAVVGDTVGDPFKDTSGPSMNILIKLMSVVALVFA
                     PVFAAIHG"
     gene            7876..8592
                     /locus_tag="VFD74_09250"
     CDS             7876..8592
                     /locus_tag="VFD74_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZK49766.1"
                     /translation="MSEAEGKRIPDRAAREYLKQIFKLEEREGPREGPVQTSELAHAL
                     GLTAPAVTDMAKKLAERGFVDYTPYHGLRLTAAGRREATRALRRHRIVERLLTDMLGF
                     PWSDAHELAVQFEHEMPEAVEERLYEALHRPADCPHGFPIPDVPEDSFPDVMTLDELR
                     PGEVSEVATVLEEGPDILGFIESLGLRPGARVEILQRRTLDGPLVARVAGVERTVGEN
                     LARIVTVRPVVRSGGSGVRR"
     gene            8758..10266
                     /locus_tag="VFD74_09255"
     CDS             8758..10266
                     /locus_tag="VFD74_09255"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZK49767.1"
                     /translation="MHAASQTAIDKQHAKGKLTARERIDLLLDEGSFVEMDMFARHRS
                     TNFGMDADRPYGDGVVTGHGTVDGRLVFVFSQDFTIFGGSLGEVFADKICKVMDLAMK
                     MGAPCLGINDSGGARIQEGVVSLAGYANIFYRNVDASGVIPQISLIMGPCAGGAVYSP
                     AMTDFIFMVEGTSHMFITGPNVIKTVTGEDVSFEDLGGAMTHNSRSGVAQFAATNEHE
                     CIAEARRLLSFLPSNNVEDPPVRAATDDPNRTSPFLQAIIPDDANAPYDMHDVIREVF
                     DDGDFMEVHKYFAPNILVGFARLNGHSVGVVANQPAHMAGTLDIDSSRKGARFVRFCD
                     AFSIPLVTFVDVPGFLPGIDQEWGGIITHGAKLLYAFCEATVPKLTVITRKAYGGAYD
                     VMNSKHIRADFNFAWPTAEIAVMGPQGAANILYGKELAAIEDPEEKARRRQELVTEYE
                     SRFANPFIAAERGYVDDVIQPADTRPLLVRALERCLTKREDHPPRKHGNIPM"
     gene            10280..10606
                     /locus_tag="VFD74_09260"
     CDS             10280..10606
                     /locus_tag="VFD74_09260"
                     /inference="COORDINATES: protein motif:HMM:NF016187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HZK49768.1"
                     /translation="MDIYTEGVAIMIIGMGVVFITLAVLAAFTGLLERVFRPREDRPL
                     TASNGDLGDGLPAEAHDAAQGFEDMALMAVAIHLIQKQRQQTYTPLARVSEQWKLVGR
                     FKSLRR"
     gene            10613..>10838
                     /locus_tag="VFD74_09265"
     CDS             10613..>10838
                     /locus_tag="VFD74_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49769.1"
                     /translation="MKYKMKMSGTEHEVRVVEVDLDLYRVYIDDQEFDVEVESDAARR
                     PAPAFMPAAPAVGTAAHQASQAAPAPAPAPA"
BASE COUNT         1754 a         3490 c         3613 g         1981 t
ORIGIN      
        1 gccctacttc gtggaatacg gctcctacgt cgacaatcac ggtggcgtcc tccgcctggt
       61 ggacaccaac cccgacttca gtctcgacct cgaccgcatc tcggcggcca tcacgccgaa
      121 gacccgggcc gtcattctca actcgcccaa caaccccacc ggacgcatct atccggagga
      181 gaccgtctgt gagttgggca ggcttctcga gcgcaaggga gccgagacgg gacgcatcat
      241 cttcctgatc gcggacgagc cgtaccgcaa gatcgtgtac ggagacacag cggtgccttc
      301 tctgatggca gcctacaaga acaccattct tgcgaccagc tactccaaag acctctcctt
      361 gtcgggggag cgcatcggtt tcgtggccgc gggctcgggc atccgtgaca tccagaaact
      421 catgggtggg ctggtgctcg cgaaccgtat cctgggcttc gtgaacgcac ccgctctcat
      481 gcagcgggcg gtggcgaggc tacagggagt gcacgtggat ctaaccttct acaagcagaa
      541 ccgcgacctg ctcctgggtg cgctccgcgg ctcggggttc acggtgccgg agccggacgg
      601 ggccttctac ctcttcccca agtcgccgat cgaggacgac gtggccttcg cgcaggagtt
      661 gcaggagcgc ctcgtgctgg tggtgccggg cacgggcttc ggggggccgg gtcacttccg
      721 catcgcctac tgcgtctccc ggcagaccgt cgagggcgcc atccccatct tcgaggagat
      781 agggcacaag tacttcgggt agccggagtc ggcggtcgcg caggccacgg cggtgacgat
      841 gacgatagca agaggagtgc ggtccctcgc cggcgcgtga tcgcgcggct ctcggcccag
      901 ttccccgata acttcactca cccgcaagat cgtactcccg tgactccgga ctctcggggc
      961 ggagcgttca ttcgccgggg agctctcggt agctgacggc acggatctcc atccggtata
     1021 cgagagtccg ggcgatggtc ctggggtcga gcgggtcggt cacggcttcg ggatggtact
     1081 tggcgatgac ggcgcggagc gccgcgtcct tctggagagg cgagtccaac cgcgtgaccg
     1141 ctccaaagac cagtgccgtg cgagagtcga cgttgtctcc gcacggtgtg tcgcccttga
     1201 tgagatcgga gccgctcatc acggcaagac aggcgcgagg gtcggtttcg agggcgcgcg
     1261 acttcttgcc ttcgccgccg tgcaggtaga tgtcttcacc atcgtagacg aacgagacgg
     1321 ggacgacgta gggcccgtcg ttgtcaccga cggccaggaa gccgacgtct tgggaagcca
     1381 gtagctccag cgccttggct cgggattccc ccgcggccgc ttgttcgtcc aaacgggcct
     1441 gtagctcctc atcgatcacg ttgttcctct ctcttggtat gtggtgtacg ggtgcgacgt
     1501 gcccggcgca cgtcgttcgg cggattcatc gtgcgttgat cgccctcacg tgtcgctcga
     1561 tggcgacgct gtagccgacc cccgggactg cccaacgacc gtccagatcg gagagggtcc
     1621 gtgctgctcc cctgggggac acgctatcgt accgcggatc ggtggaggag ttggtccagg
     1681 cagcctggtc ggccaagaac acgtaggcca ccatgtgggc cacgtgagcc tgcactcccg
     1741 cctcggctgt ggggaaggca agtcccggag ccccgttgcc cgtggctccg atgcctgcga
     1801 agttgttctg ctccgggcgc acgctgccgg tgtaggagcc gaatcctgtc tcgtgcagca
     1861 tctgtgccca caagatgtcg gctcgcagcc cgacttcggc tgccaaatgc acgtagtagc
     1921 cggcgagagt cgacgcgggc accgtgaacc gtggcgaggg cacggagacc agaaggtagc
     1981 gggagagttc tgcggcggtc cccgcccaga cgccccggag cggagtggcc ccgccgtcga
     2041 ggaccgtgcg cgtcggcgtg gacgaagcgg cggaccgggc gccgggagca gccgtcggtt
     2101 ggagggccgg atcgaaggcg atcaaggcca cggcgcccac ggagtcggtg tggccttcag
     2161 cgcgcagcag gctccgctcg acttcgcgtt gcttggaccg ccagggcggt tcctggggtg
     2221 tggcagcgag ccgggatggc gactccgccg acgggtccgc cggggtcggg tgactcgtgg
     2281 cagggtggtt ggtggcgagg tcgatcgcga cccctgaggg cgctgcgagg ggtgccgggt
     2341 ccgcgacgta gaccgccgcc agcgcgagtg cgctcccagc cacgacgagc gcggctccga
     2401 tgatcacggc gatcgaacgt gttgacgggt gtttcaccgg ccggcggggc ctcccggaca
     2461 tggtgtcccg acggcgaccg agccgtgcgg gcgagatgca acatgatacc tggatataat
     2521 ggggcgcgtt gccggcgggc gccgcttcgc actaccgtgc gtggcgcccc gctcggatac
     2581 ccagggggcg ctcttgtcgg ctccgtgtcg agggtcgcag gtcggaggat ccgagtgaag
     2641 tatgtggtct tgataacaga cggcgcatcg ggatggccgg tggagacttt cgcgggccgc
     2701 accaccctcg aggcggcgga gatgcccaac ctggatcgca tggcccggac tggggtcgtg
     2761 ggtcttgcgg ccacggtccc ggatggcatg gagccatcga gtgcggtggc ctgtatgagc
     2821 gtcatgggct acgacccgtc cctgttctat gcggggcggg ggcccatcga agcgcttgcc
     2881 ctcagcatcg acctcgaacc gggtcaggtc gcgctccgct gcaacctggt gtgcgtggac
     2941 gacggggcga tgcgcagcta ctcagccggc aacatctcct caagcgagtc gcacgtgctg
     3001 atggcggcga tccaatccga actcggagat cggcgcgtgc ggttccatcc gggcgtgggc
     3061 tttcgccata tcttgacagt gcgcgagggt cgggaccttc tggcgacccg ctgtgtcccc
     3121 ccgcacgatc tggctgatca gcagatcggc gggcacctgc ccgacggtcc ggcggcggac
     3181 cttctgctcg atctcatgga acgctctaaa cccatcttgg cggcgcaccc ggtcaatcgc
     3241 ggacgcaagg accgcggcga gctgccggcg acgcagatat ggccgttctg gcccggcgtg
     3301 cggcccgcgg ccatgccgtc gttccgcgag aagtacgggc tgcgcgcggc actcacctct
     3361 cccgtcgacc tgctgcgggg catcgccaat cagctcggca tcgatgtgct cgagatcgcc
     3421 ggggtcaccg acgggccgga caacgactac ggcgctcaga tgaagggagc cctcaaggcg
     3481 ctcggcgacc atgatctcgt gatcgtgcac gtggaggcgc cggatgccgc cggacatgac
     3541 ggtgacgccg agggcaagcg caccgcgctc gagcgcatcg acgtcgagat ggcttcgcag
     3601 ttgtgggagc gggaaggtgg gctgcgggtg ctggtgatgc cggatcatcc gacacctttg
     3661 gcactgcgca cgcacgcggc cgagccggtg ccgttcatca tatggggtga gggatcgacc
     3721 gggacaccgg ccccgtcgtt cacggagagt gccgcggccg gcaccggtct tctggtgccg
     3781 gccggccacg aactgatggc gatgttgctg ggctagttct ccatcttctc gaagtcttct
     3841 ttgctggcgc cgcaatccgg gcactcccaa tcatcgggca gatcctcgaa cgccgttccc
     3901 ggggcgattc cctgcgaggc gtcacccacg gcggggtcgt agacgtagcc gcagagcatg
     3961 cattcccatt tgtccatgtt cgactccttc ggtctgtcgg ccgcagatcg gttgcgtcgc
     4021 gcccatggta accgcttggc ggactcggta aacgcgcaac gcccgtccag cccgcgttcc
     4081 ggcttgatca cgggtcgatc gggcttgaca aaggcccatg cgggctagta gcctgcggag
     4141 gtcccaaagt taaggccgct taatagacac ggtcgggtgt gccatgcacc cgggaggaac
     4201 tcccagggag gggatcggac acacttgatg cgcgtcagct gtttgcagtc gatgtctgag
     4261 gatccaggcc gggcgcccga tgcctgaaga gttgggcgaa gctctcagta tctggttggt
     4321 cctgcctttc gcggggctgc tcctctcgat cgccatactc ccgttgacgg cggagcattg
     4381 gttcgagaag aatcgcaacc gggccctcgt ggccggtctc ttcggcgttc cggtcgtggt
     4441 gtacctcctg gcccgcttcg gtgccgaggg tctcgccgag gtgctgagta cgggggagga
     4501 gtacatctcg ttcatcgtgc tcttgaccgc gctcttcacg atctccgggg gcatctacct
     4561 gacgggcaac ctcctcggca cccctttgac gaacgtggct ttcttgctgg tgggcgccgt
     4621 cctggccaac ttcatcggca ccaccggcgc ggccatggtg ctgatccgcc ccctccttcg
     4681 ggcgaacagc gagcgccaca accagaaaca cgttgtcatc ttcttcatct tcgtcgtggc
     4741 caacatcggt ggacttctca cgcctctcgg cgacccgcca ctcttcttgg gcttcttgag
     4801 gggcatcgac ttcttctgga ccatgaggtt gcttccgcaa tgggctcttg cggtgggact
     4861 caccgtcgcc atcttcttca tcctcgacta tcgtgcctac aagagcgaga agcccaagtg
     4921 gatcagggaa gacatcgcgg actacgtgcc tatgcgcatc gccggcaaga ccaacctcct
     4981 cttcctggcg ggggtcatcg ccgcggtgct tctgtcgaag cagttggccc aggtggggga
     5041 gtccatacac ttcccgttcc ttcgcgaact tatcatgggg gcgatgatcc tgctctcgct
     5101 caaagtcggc ccgagcggcc cgcgaaaggc caatcacttc agctgggcgc ccatccagga
     5161 agtcgccatc atcttcgccg gcatctttgc ggcgatgatc ccggccctcg ccatcttgaa
     5221 ggcgcgcggc ggggaactcg gattcacgga gccctggcac ttcttctggg ctacgggggt
     5281 gctctcctcg ttcttggaca acgctcccac ctacctcacc ttcacctcga cggcgcaggg
     5341 cttcctgggc atcaacgagg ttcccggcct catgggctcg gggatcatcg agtcgttggg
     5401 ccgagcgccg gcggcgtttc tggcggccat ctcgtgtggg gccgtgttca tgggagcgaa
     5461 ctcatacatc gggaacgcgc cgaacttcat ggtcaagtcg atcgccgagc gcagcgggca
     5521 gaagatgccg tcgttcttcg gctacatggg ctactcgttc gtggtgctcg tgccggtgtt
     5581 catactcgtg actctggtct tcttcaggtg atcggcaggg ggtgcgaaca gaggacagtc
     5641 ggggtgtcgt aggtttcgaa gaggccgctc tgcaggcggt ctgtccgtag gaagtggtgg
     5701 cgcgccggtg ggcggcgcga tcacgtcgat tcgaaggagg atctcgatgt cgttggaaac
     5761 gtgggcggtt cttgctgctg ccgcagccgt cctcggtctc atctacgcag cctacacggc
     5821 cagccgtttg gtgaaggcgg aagtgggcac cgagaagatg aggaccatca tgggtctcat
     5881 ccaaagtggc gccatggcgt tcttgaagcg cgagtattcc gtattgatct ggttcgtcgg
     5941 tttcgtcgcc atcgcgatcg ccgtggtcgg cgcgatctgg gacgtcggcc tcggatggca
     6001 gaccgccatc gcatacctgt gcggtagctt cctctccgcg tccgccggtt acctgggcat
     6061 gatgattgcc acacgcgcga acgcgcgtac ggccaacgcc gcgcgcacct cgctcgcggc
     6121 agccctcgat gttgccttca agggcgggtc ggtgatgggg atgatcgtgg gcggcctggg
     6181 actgctgggc gtctccgtga tctggctcat cttcagggaa cctcagtatg tcaacggctt
     6241 cgcgctcggc gcgagctcca tcgcgctctt cgcccgcgtc ggaggcggca tctacaccaa
     6301 ggccgccgat gtgggttccg acttggtggg taagatcgaa gccggtatcc cggaagacga
     6361 tccgcgcaac cccggcgtca tcgccgacaa cgtgggcgac aacgtgggcg acgtggccgg
     6421 catgggtgcc gacctctacg agtccttcgt gggctccatc atcgccacca tggcgcttgg
     6481 cgtggtgctg gcgaacaacg ccaccaccag caagttcgtc atcctgcccc tgatcatcgc
     6541 gggtgcgggc atcctggcct cgatcgtcgg cacgctcttc gtcaaggcga aggaaggagc
     6601 gcgccttcac accgccctca acctgggcac gtacgtcgcc accgctctga ccatcgccgc
     6661 gatgttcgtg accatcggct ggttcctccc cggtgacggc gagagctgga ccacgtacat
     6721 gggcttcttc tggggcagcg ccggcggtct cttggcgggc gtgctgatcg ggaaggtgac
     6781 cgagtactac acctccgacc actacaagcc tgtcaagggc atcgccgaag cttcccagac
     6841 cggagccgcc accaacataa tccgtggact cggcgtaggt atggagtcgg tcgtggtgcc
     6901 cgtactcctc gtcgtgggcg cgatcatcgt ggcctactac ctgggggatc gcgtcccgct
     6961 caaggactcg ggcgtctacg gggtcgctct tgcggcgctt gggatgctct ccaccaccgc
     7021 catgaccatc agcgtcgatg cgtacggccc gatcgccgac aatgccggcg gtatcgccga
     7081 gatggccgaa ctgccgaagg aagtccgcgc ccgcaccgac caactcgatt cgctcggcaa
     7141 cacgaccgcc gccatcgcca agggttttgc catcggctcc gccgctctga ccgccctggc
     7201 gctgttcacc gctttcgcgg agacctacaa gacgtccggc ggcggcgaac tgaccctcga
     7261 cctgttgaag gggccggccg tgatcatcgg cctcttcctg ggtgccgccc tgccgttctt
     7321 gttctcggcc ctcaccatga acgcggttgg ccgcgccgcc gcttcgatca tcgacgaggt
     7381 gcgccgccag ttccgtgaga tccccggcct gatggaaggc accgcggtgc cggattcggc
     7441 cacctgcgtc tcgatcgtga ccggctcggc gttgaaagag atgatcctcc ccggcgttct
     7501 ggccgtggtc gcgccgctgg tggtgggcct gatcagtcct gtcgctctgg gtggtctgct
     7561 ggccggtgcc ctcgtgaccg gtttcctgct cgccatcatg atggccaacg cgggcggcgc
     7621 ctgggacaac gccaagaagt tcatcgaagg cggcgagtac ggcggcaagg gttcggcgcc
     7681 gcacgcggct gccgtcgtgg gcgacacggt gggtgacccc ttcaaggaca cctccggccc
     7741 gtccatgaac atcctcatca agttgatgag cgtggtggcg ctggtgttcg cgcctgtgtt
     7801 cgcggccatc cacgggtagc cgagcgacgc gcgcaaagtc cacgtcctgt caccgaacgc
     7861 atcgagccgg ctgacatgtc agaggccgaa ggcaagcgga tccccgaccg ggcggcccgc
     7921 gaatacctca agcagatctt caagctcgag gaacgcgagg gcccccggga ggggccggtg
     7981 cagacgagcg aactagccca tgcattgggt ctgaccgctc ccgccgtcac cgacatggcc
     8041 aagaagttag ccgaacgtgg gttcgtggac tacacgccct accatgggct ccgcctcacc
     8101 gccgcgggga ggcgcgaagc gacgcgggcg ctgcggcgcc atcgcatcgt ggagcgccta
     8161 ctcacggaca tgctcgggtt tccctggtcg gacgcccacg agctcgccgt ccagttcgag
     8221 cacgagatgc ccgaggcggt ggaggagcgg ctgtacgagg cgttgcacag gcccgctgac
     8281 tgcccgcacg ggttcccgat cccggatgtc cccgaggact cgttccccga tgtcatgacg
     8341 ctcgacgagc ttcggcccgg cgaggtgagc gaggtggcga cggtgctcga ggaggggccg
     8401 gacatcctgg gcttcatcga gtcacttggg cttcgtcctg gagcccgggt ggagattctc
     8461 cagaggcgga ctctcgacgg cccgctcgtg gcccgcgtgg ccggggtcga acggacggtg
     8521 ggggagaatc tggcccgtat cgtcaccgtg cgccccgtgg tacgctcggg tggctcggga
     8581 gtgcggcgat agacgggttt tcgcaccagg tccagcgggc atctcatcct gagcacttat
     8641 ggcatcatgc ctcgcggacg gtgtagcgcc tgcctcgttc tcatcacaag gagttgaaac
     8701 ggcttggaca cgacgaaggg aaagctccgc catctgcgtg aggtgaagga cgccgctatg
     8761 cacgcggcct cgcagacggc gatcgacaag cagcacgcca agggcaagct cactgcccgc
     8821 gaacgtatcg acctgctcct ggacgagggc tcctttgtcg agatggacat gttcgcgcgc
     8881 catcgctcga ccaacttcgg catggatgcc gatcggccct acggcgatgg cgtggtcacc
     8941 ggacacggca ccgtggacgg tcgcctggtg ttcgtcttct cgcaagactt caccatcttc
     9001 ggcggatcgt tgggcgaggt gttcgccgac aagatctgca aggtgatgga cctggccatg
     9061 aagatgggcg cgccgtgcct cggcatcaac gactcgggcg gcgcgcggat ccaagagggt
     9121 gtcgtctctc tcgcgggcta cgccaacatc ttctaccgca acgtcgacgc gtcgggggtg
     9181 atcccccaga tatcgctgat catgggtccc tgtgccggtg gagccgtgta ctcgccggcc
     9241 atgaccgact tcatcttcat ggtcgaaggc accagccaca tgttcatcac agggccgaac
     9301 gtcatcaaga ccgtgaccgg tgaggacgtt tcgttcgagg acctgggcgg cgccatgacc
     9361 cacaactcgc ggagcggggt ggcgcagttc gcggccacga acgagcacga gtgtatcgcc
     9421 gaggcccgcc ggctcctctc gttcctgccg tcgaacaacg tcgaggatcc gccggtccgc
     9481 gcggccaccg acgatcccaa ccgcacgtct cccttcctgc aggcgatcat ccccgacgac
     9541 gcgaacgccc cctacgacat gcatgacgtg atcagggagg tcttcgacga cggagacttc
     9601 atggaggtgc acaagtactt cgcacccaac atcctcgtcg ggttcgcccg gttgaacggc
     9661 cactcggtgg gagtcgtggc caatcagccg gcgcacatgg ccggcacgct ggatatcgac
     9721 agttcgcgta agggagcgcg tttcgtgcgg ttctgcgacg cgttcagcat cccgctcgta
     9781 accttcgtcg atgtgcccgg cttcctgccc ggtatcgacc aggagtgggg cggcatcatc
     9841 acccacgggg ccaagctgct ctacgctttc tgcgaggcga cggtgcccaa gctgacggtg
     9901 atcacccgca aggcatacgg cggtgcgtac gacgtcatga actcgaaaca catcagggcc
     9961 gacttcaact tcgcgtggcc gacggcggag atcgcggtga tgggccctca gggcgccgcc
    10021 aacatcctct acggcaagga actcgccgct atcgaggacc cggaagagaa ggcccgccgc
    10081 cggcaggaac tcgtgacgga gtacgagagt cgcttcgcca atccgttcat cgcggccgag
    10141 cgcggctacg tggacgatgt gatccagccc gctgataccc gtccgttgct ggtgagggcg
    10201 ttggagcggt gtctcacgaa acgtgaggac caccccccgc gcaagcacgg caacatcccg
    10261 atgtaggccg gagcgctcgt tggatatcta caccgaaggc gtcgcgataa tgatcatcgg
    10321 catgggggtg gtcttcatca cccttgccgt gctggcggcg ttcaccggcc tgctggagcg
    10381 ggtcttccgc ccgcgcgagg accgacccct caccgcgtcg aacggcgatc tcggcgacgg
    10441 tctgcctgcc gaagctcacg acgccgcaca gggattcgaa gacatggctt tgatggcggt
    10501 cgccatccat ctgatacaga agcagcggca gcagacatac accccactcg ccagggtcag
    10561 cgagcagtgg aagctggtgg gtcgcttcaa gtccctgagg aggtaggctc tggtgaagta
    10621 caagatgaag atgtccggga cggagcacga agtccgggtg gtggaagtgg acctcgatct
    10681 ctaccgggtg tatatcgacg accaggagtt cgacgtggag gtggagtccg acgcggcgcg
    10741 gcggccggcc ccggccttca tgccggccgc cccagccgtc gggaccgccg cgcaccaagc
    10801 ttcccaagct gcccctgcgc ccgcacctgc gccggcgt
//
LOCUS       DASRMJ010000333         5400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_210989, whole genome shotgun sequence.
ACCESSION   DASRMJ010000333 DASRMJ010000000
VERSION     DASRMJ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210989"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VFD74_09270"
     CDS             complement(<1..1063)
                     /locus_tag="VFD74_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK49770.1"
                     /translation="MAKYVIVGNGVTGTRAAEVIRRADGSADVSILTEEPHPFYRRPQ
                     LADFASGAIGETRMWVRKASFYEDHRLDLRLSTKVVAVDVDSHLLTLADGATVPYDRL
                     LVATGRYVTSFGVPGGEGGGINHFKTLDEARTVREFDAAGKTGVVFGNGMVALELVRA
                     LTSAGFSTTYLVPTDTLWPEALDKDAADIAVSRLHAAGARVVFGARIGSVATSGGCGG
                     EVSLADGDVLRADFMGLCSDLTPAVDFLPGGGAGLVVGLDFSTPWADVYAAGDVTAGE
                     GKSYFNWLRSWRQGEAAGAAMVGDVVESQAAVDTLNMQVLGLSLVALGRTVVPYRSAD
                     SEMRGDYPYGEFYKKLVFSA"
     gene            1316..2149
                     /locus_tag="VFD74_09275"
     CDS             1316..2149
                     /locus_tag="VFD74_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK49771.1"
                     /translation="MTRPTPNIPLTSPFDSQLDLLADALGDPTRRAVFKYVAESAEAV
                     TAADVGATFGVHRTVARAHLERLSDTGLLVSDFLHRPEGGRPPKVYRRSETRLDLQEP
                     VRQYQMLAELLLQALARFGEAGELLVGEIGRDFGRGLADAAGDVDSIEDLLAPLSRAG
                     ADIHVETEADRVVVRTGNCLFLEVATKQPRLVCLLDKAIMTGLLSGGDRPLVLTDHQR
                     RGGREACTLVFEAVENGLGEQIAQEAIEAQARSAESRAVEIHPIGGDDEGRARNAEEQ
                     A"
     gene            2146..2793
                     /locus_tag="VFD74_09280"
     CDS             2146..2793
                     /locus_tag="VFD74_09280"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZK49772.1"
                     /translation="MIITKQKPIEEISQMLERHKNLFFVGCGQCSTQCQTGGEWEVEE
                     LGKILEEDGHHTVGSSVVYAPCHELDVKRHFRKHKEQLAKADAVVVLSCGAGVQSVVE
                     ATDLPVYPSNDSLFLGNSQRHMHFEEKCSTCGECVLDWTGGICPVTRCPKGLLNGPCG
                     GTNHGKCEVDSDKPCIWVEIYKRLDAQNDLDKFREIRPPKNHSAIKRPGLMRGEH"
     gene            complement(2865..4073)
                     /locus_tag="VFD74_09285"
     CDS             complement(2865..4073)
                     /locus_tag="VFD74_09285"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HZK49773.1"
                     /translation="MERYLLKEDIWWVGAIDWNMRDFHGFETPRGTTYNSYLITDQKI
                     TLVDGVRSGFASETLRRISTCCDPRDIDYLVVNHVEMDHSGALPELIRRASPVKVFCS
                     KRAKDALAIFYAEEGFGDWDINVVGTGDEIVLGRYTLTFLEVPMLHWPDSMLTYVKEA
                     KVLLANDGFGQHLATSKRFADEVDMAIVMDEAAKYYGNILMPFGNQVQKLLKTVGDMG
                     LELDIVAPSHGVIWRRPEDIEAIVRCYSEWSSFEARPSVALIFDTMWHSTEKMTRAIE
                     DGIAQEGVECSVIKLSKTPLAEAARAVQTCRAFLVGTPTVNNGMLPSVGAFMTYIKGL
                     RPKQRLVGAYGSFGWGGGGVKQVDAGLRELGLDVIDPLEFKYVPSSEDLVACEEYGRE
                     VARRVKAWEV"
     gene            complement(4203..4700)
                     /locus_tag="VFD74_09290"
     CDS             complement(4203..4700)
                     /locus_tag="VFD74_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZK49774.1"
                     /translation="MADTMKNLAEAFAGESQANRKYLAFAKRAEEEGLAEVAKRFRVA
                     AASETVHAHNHLKVMGELKDTAANLAASIGGENHEHTAMYPEFMEIAAKEGGQDAKFS
                     FWAANEVEKFHESMFAEAGEKMGDLPSSRFFVCEVCGMTYEHEAPDACVICSAPRFRI
                     PEFTA"
     gene            complement(4912..>5400)
                     /locus_tag="VFD74_09295"
     CDS             complement(4912..>5400)
                     /locus_tag="VFD74_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZK49775.1"
                     /translation="DMIDVGGAVGHMARAFADRGIRATLFDRDDVLPEARDFLGAGSE
                     GIDMQGGDFTVSLPAGPFDLAYLGNVFHIYGPETLQALTRRVFEVLRPGGVICVRDLV
                     WERSPRAAMFAVNMLQATAEGGVWREGQYHTWLGGAGFEDIQVVDLGLSASQLVLGRR
                     PR"
BASE COUNT          965 a         1875 c         1667 g          893 t
ORIGIN      
        1 ccgccgagaa caccagcttc ttgtagaact caccgtatgg gtagtcgcct cgcatctccg
       61 aatcggccga gcgatagggc accactgttc gcccgagtgc gacgaggctc aagccgagca
      121 cctgcatgtt cagggtgtcg accgccgcct gactttcgac gacgtcgccc accatggccg
      181 cgccggccgc ttccccttgt cgccaggaac gaagccagtt gaagtagctc ttgccctcac
      241 ccgccgtcac gtctccggcg gcgtacacgt cggcccacgg cgtggagaag tcaagaccca
      301 ccaccaggcc ggcgccccca ccgggcagga agtcgaccgc aggggtcaga tcggagcaca
      361 ggcccatgaa gtcggcacgc agtacgtcac cgtcggcgag gctcacctcg cccccgcacc
      421 cgcccgatgt ggcgaccgat ccaatgcggg ctccgaaaac gacgcgggct cccgcggcgt
      481 ggagacggct cacggcgatg tcggccgcgt ccttgtcgag ggcctcgggc cacagcgtgt
      541 cggtgggtac gagatacgtg gtcgagaatc ccgcggaagt gagggcgcgg accaactcaa
      601 gcgccaccat cccgttcccg aacacgacgc cggtcttgcc ggcggcatcg aactcgcgca
      661 cggtacgagc ctcgtcgagg gtcttgaaat ggttgatgcc gccgccctcg ccgcccggca
      721 cgccgaacga cgtcacgtag cgcccggtag ccaccaggag tcggtcatag ggcactgtgg
      781 ccccatcggc aagcgtgagg aggtgcgaat cgacatcgac ggccacgacc ttcgtggaca
      841 gccgaagatc caatcgatgg tcttcgtaga acgatgcctt gcgcacccac atgcgggttt
      901 cgccaatcgc acccgacgcg aaatccgcca actgcgggcg ccggtagaaa ggatggggtt
      961 cctctgtgag gatgctcacg tcggccgatc cgtccgcccg cctgatgacc tcggcggccc
     1021 gtgtgccggt cacgccgttc ccgacgataa cgtacttcgc catcccaaca ctccttccgt
     1081 gcacccgtta gcggcctgcg gcagcagccg agccattgta ccagcagact ccgacgcccg
     1141 cccacggccc gcaccgccca cggcccccgg cggagcgata aggccggcgc cgaaccgtcg
     1201 atttcggcgg taaccctttg gagccgggcg cgctcccccg tgccatccga ggttacgtgc
     1261 atattcgcac accaaacccc ttgcagcccg ggtttctgtg ggatagactg accatatgac
     1321 gcggcccaca ccgaacattc cgctcacatc gccgttcgac tctcagctcg acctgctggc
     1381 cgacgcactc ggcgatccca cgcgcagggc cgtgttcaag tacgtcgcgg agtccgccga
     1441 agccgtgacg gccgcagacg tgggcgccac cttcggagtg catcggaccg tggcgcgcgc
     1501 gcaccttgag agactctccg acaccggcct gctcgtctca gacttcctcc atcgtcccga
     1561 gggagggcgg ccccccaagg tgtaccggcg aagcgagacc cggctcgatc ttcaagagcc
     1621 cgtccgccag taccagatgt tggccgagct cctgcttcag gcattggcgc ggttcgggga
     1681 ggcaggggaa ctcctcgtgg gtgagatcgg gcgcgatttc gggcgcggac tcgccgacgc
     1741 cgcaggtgac gtcgactcca tcgaagacct cctggcgccg ctctcccgcg cgggcgccga
     1801 catccacgtg gagaccgagg ccgaccgtgt tgttgtgcgc accggcaatt gcctgttcct
     1861 cgaggtcgca acgaaacagc cgcggctcgt ctgccttctc gacaaggcga tcatgaccgg
     1921 actgctctcc ggcggtgatc gtcccctggt tctgacggat caccagcgcc gcggcgggcg
     1981 cgaggcctgc accctcgtct tcgaggccgt cgagaacggc cttggtgaac agatcgcgca
     2041 ggaggcaata gaggcccaag cccgctcggc ggagagccga gcggtcgaga tacatcccat
     2101 cgggggcgac gacgagggtc gtgccagaaa cgcggaggaa caagcatgat catcaccaag
     2161 cagaaaccga tcgaagagat ctcgcagatg ctcgagaggc acaagaacct gttcttcgtc
     2221 ggctgcggac agtgctccac ccagtgccaa acgggcggcg agtgggaagt ggaggagctc
     2281 ggcaaaatcc tcgaggagga cgggcaccac accgtcggat cgtccgtggt gtacgcgccc
     2341 tgccatgagc tcgacgtgaa gcgccatttc cgcaagcaca aagagcaact tgccaaggcc
     2401 gacgccgtcg tggtgttgag ctgcggcgcc ggtgtgcagt cagtggtgga agccaccgac
     2461 ctgcccgtgt atccatcgaa cgacagcctc ttcctcggca actcgcaacg acatatgcac
     2521 ttcgaagaga agtgctctac ctgcggcgag tgcgtgctcg actggaccgg cggcatctgc
     2581 ccggtgaccc gttgccccaa gggtctgctc aacggaccct gcggcggcac gaaccacggc
     2641 aagtgcgaag tcgacagcga caagccctgc atatgggtag agatctacaa gcgcctcgac
     2701 gctcagaacg atctggacaa gttccgggag atccgcccgc ccaagaacca ctcggccatc
     2761 aagcgccccg gcctgatgcg cggcgagcac tagggcccgt ctcgggagct acatcggggc
     2821 cacacggggg gccgcatccg cgacccccga tcgccgttcg gagctcagac ctcccaagcc
     2881 ttgaccctgc gggcgacctc tcgcccatac tcctcgcagg cgaccagatc ctcactggac
     2941 ggcacgtact tgaactcgag cggatcgatc acgtcgagtc cgagttcgcg caggcctgcg
     3001 tccacctgct tcacgccccc gccgccccag ccgaacgagc cgtacgcacc cacgaggcgc
     3061 tgcttcggac gcaacccctt gatgtaggtc atgaaggccc ccaccgatgg caacatgccg
     3121 ttgttcaccg ttggagtgcc cacgaggaac gcgcggcatg tctggacggc ccgcgccgct
     3181 tcagcaaggg gggttttgct cagcttgatc accgaacact ccaccccctc ctgcgcaatc
     3241 ccgtcctcga tggcccgcgt catcttctcg gtggagtgcc acatggtgtc gaagatcagg
     3301 gccaccgacg gtcgtgcctc gaacgagctc cactccgagt agcatcggac gatcgcctcg
     3361 atgtcctcgg gccgacgcca gatcacccca tggctcgggg ccacgatgtc gagttcgagc
     3421 cccatgtcgc ccacggtctt caagagcttt tgcacctggt tgccgaacgg catgaggatg
     3481 ttgccgtagt acttggccgc ctcgtccatg acgatggcca tgtcgacctc gtctgcgaac
     3541 cgcttggacg tggccagatg ttgaccgaac ccgtcgttgg ccagcagaac cttggcctct
     3601 ttgacgtagg tgagcatgct gtcgggccag tggagcatcg gcacttcgag gaaggtgagc
     3661 gtgtaccggc cgagcacgat ctcgtcccct gtgcccacga cgttgatgtc ccagtcgccg
     3721 aacccttcct cggcgtaaaa gatggcgagc gcgtccttgg ccctcttcga acagaagacc
     3781 ttcaccggcg acgcgcgtcg gatcaactcc ggaagcgccc ccgagtgatc catctccaca
     3841 tggttgacca ccagatagtc gatgtcccgc ggatcgcagc aggtgctgat gcggcgcagg
     3901 gtctccgatg cgaagcccga cctgaccccg tcgacaaggg tgatcttctg gtcggtgatc
     3961 aggtacgagt tgtaggtggt gccgcgggga gtctcaaaac cgtggaagtc acgcatgttc
     4021 cagtcgatcg ccccgaccca ccagatgtct tccttgagca gatagcgctc catgaaccac
     4081 ctctgattcc gtgctctatg tgggaccggc gaccccatcg ggggccgcca gccaacagta
     4141 tacgggaggc gcgcggccga ggttccgaag ctgacggatc cggccgtggc ctcccgggag
     4201 gcctacgccg tgaactcggg gatgcggaac ctcggcgccg aacagatcac gcaggcgtcg
     4261 ggagcctcgt gctcataggt catgccacac acctcgcaca cgaagaagcg gctcgacggc
     4321 aggtcgccca tcttctcgcc ggcttcggcg aacatgctct cgtggaactt ctccacctcg
     4381 ttcgcggccc agaacgagaa cttcgcgtcc tggccgccct ccttggcggc gatctccatg
     4441 aactcggggt acatggcggt gtgctcgtgg ttctcgccgc cgattgaagc ggccaggttg
     4501 gcggccgtgt ccttgagctc gcccatgacc ttgagatggt tgtgggcgtg cacggtctcc
     4561 gaagcagcag ccacgcggaa gcgtttcgcg acctccgcca agccttcttc ctcggcccgc
     4621 ttcgcgaacg ccaggtactt ccggttcgcc tgggactccc cggcgaacgc ctcggctaga
     4681 ttcttcatgg tgtcggccac agttgatctc ctctcggtgc tttcgactcg cggccctcgc
     4741 atgaggcggc cctcgcatga ggcgcactcg cacgaagacc cggtcgtcac gatgggtcgg
     4801 ccgcccgttc tacgggcgtg cccgattctc atggaaccgg gggtacggta accgctgggg
     4861 acagacctca aaccaaccca gcgccggtgc ggcgccgtgc ggcgtggtcg gtcagcgggg
     4921 gcggcgcccc aacaccagtt ggctcgctga gagtcccagg tccaccacct ggatgtcttc
     4981 gaagccggcg ccgcccagcc acgtgtggta ctggccctca cgccacaccc cgccctccgc
     5041 ggtggcctgc aacatgttca cggcgaacat ggccgcccgc gggctgcgct cccacacgag
     5101 gtcccgcacg cagatcacgc ctccggggcg aagcacttcg aagaccctgc gggtcagagc
     5161 ttgtagggtc tccggcccgt agatgtggaa cacgttgccc aagtaggcca gatcgaaggg
     5221 ccccgctggg agcgagaccg tgaagtcccc accctgcatg tcgatgcctt ccgagcctgc
     5281 gcccaagaag tcgcgcgcct ccggcagcac gtcgtcccga tcgaacaggg tggcccggat
     5341 ccccctatca gcgaacgcgc gggccatgtg ccccaccgct ccgcctacgt cgatcatgtc
//
LOCUS       DASRMJ010000334         5461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_356636, whole genome shotgun sequence.
ACCESSION   DASRMJ010000334 DASRMJ010000000
VERSION     DASRMJ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356636"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFD74_09300"
     CDS             <1..534
                     /locus_tag="VFD74_09300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZK49776.1"
                     /translation="HHHRQGGSTAAMSAPAPAPAPGSPARDTLLQAARAGLGPALIGY
                     HGTSDGALCLRVQLSGYRRAAELLRSHGIDRLDFITCVDWRHHLTLTLQGYGIGNGLQ
                     VRLSTRVARDGQEVPTVTDLWFLANWEERETYDQFGLVFSGHPDLRRLLNPDGWEGHP
                     LRKDYVDRVDINRPEYF"
     gene            582..1745
                     /locus_tag="VFD74_09305"
     CDS             582..1745
                     /locus_tag="VFD74_09305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196893.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZK49777.1"
                     /translation="MTDRSREIPERAIPEGEYQPPDDIQDFLINIGPHHPGTHGVMRL
                     VTRLDGEKVAGLDVRIGYMHRSLEKIVENRTYPQVIAFTDRTTDYLAAMHNDWVYCLA
                     AEKLLGIVVPERAEFLRVIVAEMNRITSHLMSIGALGLDSGAPTYFLWCFNARERLVR
                     LFEDICGARLTYVYMRLGGVIYDAPPGWLERVRETVRGVIDEFPSYRDLFHDNYIWHE
                     RSKGVSTLSPEEALRLGAQGPILRSTGYRWDLRKNMPYSIYERFEFDIPVGITGDVYD
                     RTEVRLAEIAESAKIIHQAIEGIPAGPILAARIPRVPSPAPGETYARLESPRGEIGCY
                     LVSDGGMKPYRMKWRGASFHNVAMLPALSVGYTLSDLINIVGTIDPVMGDVDR"
     gene            1806..3776
                     /locus_tag="VFD74_09310"
     CDS             1806..3776
                     /locus_tag="VFD74_09310"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK49778.1"
                     /translation="MTWSDLVNPWVGIARGHARTLKQMVSPPVTHMYPDEPAELSPRW
                     RGPLRLRGVMDDQRPPVTDAPPLAFNDLMTALHDEQRVAPCMGGCPANVDARGQNALV
                     AEGRYAEAYDLVRRRNVLPAVLGYVCNNPCEDVCRRSYLDDPIAIRRLHRDCYEVYDR
                     EVRFPGHLASLVHRPEHVAVVGAGPAGIAAAYDLVQMGYQVTVYEREPLPGGLLVTSV
                     PLYRLDREVVTKEIADIAELGVEFRCGVSVGVDVTLDRLRGDHDAVVVAVGYSGGRIL
                     PLPGHDAEGVWSAIDFLHAYCMGQSPRVGPEVVVIGGGDVGCDCSRSALRCGASLSVQ
                     AHVETRADMPGQDVEVLGALEEGVVLLSEWGPDEILVKDGKVAGIRLKKVVSRFDPDG
                     RWNPQYSDEVTEIRCQSVIFAVGQGLQLDFLKGSGVEFDELGRPLSEPHTGATAVDGL
                     FLAGDVATGPRTIITAIGHAHETALSVHRHIQGLGVAEDRRPPLHPPEYYLQKLYAPN
                     PPGFEHVGPGGRRVRMPESAPEERIRTGAQVELGWPRGDGRREAIRCMRCQTHVCVAC
                     TLCARVCPDNCIDVVGHDTGHERRVERYDFAMEWCCFCGFCQDVCPTQALSLAADFDY
                     AARERRDFYYDRARMLRPFAGPDETPSKDGLP"
     gene            3888..4244
                     /locus_tag="VFD74_09315"
     CDS             3888..4244
                     /locus_tag="VFD74_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZK49779.1"
                     /translation="MLASTERLDGTPLVVVTGEIDHGSSSALQSEIEPCLDGGETTIL
                     LDLKDVTYMDSGGISVLLYTVRRLRRGNGWLGVVSPNTNVRRLLKIVGLEVDSGFRVF
                     DDKESAATAIREMSTP"
     gene            4271..4645
                     /locus_tag="VFD74_09320"
     CDS             4271..4645
                     /locus_tag="VFD74_09320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK49780.1"
                     /translation="MISVAPEPIRLADVRRFVEETGAEAQLDTDKIFDLKVAVSEACA
                     NAVEHSGGQHAPLQVCAFLYSDRIVFEISDGGDFRVPGVPHRSPRDDRGLGLPLMVAL
                     MDEVRIAKTRGGGTNVHLSLFL"
     gene            complement(4679..>5461)
                     /locus_tag="VFD74_09325"
     CDS             complement(4679..>5461)
                     /locus_tag="VFD74_09325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK49781.1"
                     /translation="VLALLGVAAAGAVLFVVVESGVAHPLLDLTLFRNRLFSASTTSA
                     LASYIVTASVIFLMPFYFIQIRGFTVAHAGLLLTPIPAAMALLGPLSGSLSDRIGSRF
                     LSTTGLLVGAAGMVGLTRLGVDTGPVGILLRLLMVGVGMALFQSPNSSALMGSVPRQR
                     LGIASGVVAMARNVGMVLGVSLAALVLAVREPGYLLRPSIAALGPELSAREAFLHAAH
                     DVFWIAFVICLLGAAVSLVRGPAMAAAHPADAAGPAAARGER"
BASE COUNT          984 a         1862 c         1768 g          847 t
ORIGIN      
        1 catcatcacc ggcaaggagg aagcacagcg gcgatgagcg cacccgcacc cgcaccggca
       61 cccggatccc cggctcggga cacgctgctc caggccgcgc gcgctggact cggcccggcg
      121 ctgatcggct accacggcac cagcgacgga gcactctgcc tgcgcgtcca gttgtccggc
      181 taccgacgcg ccgccgaact gcttcgcagc cacgggattg accgtctcga cttcatcacc
      241 tgcgtcgatt ggcgccacca tctgacgctc acgctgcagg gctacggcat cggaaacggt
      301 cttcaggtgc ggctgagcac ccgcgtggcc cgcgacggac aggaggtccc cacggtcacc
      361 gatctgtggt tcctcgcaaa ctgggaagag cgggagacct acgaccagtt cggcctggtg
      421 ttctcgggcc atcccgatct gcggcgcctg ctgaacccgg acggctggga agggcacccg
      481 ctgcgcaaag actacgtgga ccgggtggat atcaatcggc cggagtactt ctgaagccac
      541 atgcgtaccc caaccgacac ccattcggcc caacgacacc catgaccgat cgcagccgtg
      601 agatccccga aagggcgatc cccgaagggg aataccaacc tccggacgac atccaggact
      661 tcctcatcaa catcgggcct caccacccgg gcacgcatgg cgtcatgcgg ctcgtcaccc
      721 gactcgacgg agagaaggtc gccggcctcg acgttcgcat cggctacatg caccggtctc
      781 tcgagaagat cgtcgagaat cgcacctacc ctcaggtgat cgcgttcacc gatcgcacta
      841 cggactactt ggcggccatg cacaacgact gggtctactg ccttgcggcc gagaagctgc
      901 tcggcatcgt ggtgcccgaa cgcgccgagt tccttcgggt catagtggcc gagatgaacc
      961 gcatcaccag ccacctgatg tcgatcggag ccctcgggct cgacagcggc gcccccacct
     1021 acttcctctg gtgcttcaat gcccgcgagc ggctcgtgcg gctcttcgag gatatctgcg
     1081 gcgcgcgcct cacctacgtg tacatgaggc tgggtggagt catctacgac gcgccgccgg
     1141 gctggctcga gcgcgtgcgc gagaccgtgc gcggggtgat cgacgagttc ccgtcatacc
     1201 gcgacctctt ccacgacaac tacatctggc acgagcgctc gaagggcgtc agcacgctct
     1261 cgcccgaaga ggcgctccga cttggcgctc agggcccgat cctgcgaagc accgggtatc
     1321 gttgggatct gcgcaagaac atgccctact ccatctacga gcgcttcgag ttcgacatcc
     1381 cagtcgggat cacaggcgac gtctacgatc gcaccgaagt gcgcctcgcc gagattgccg
     1441 agagtgcgaa gatcatccac caggcgatag agggcatccc ggcggggcct atcctcgcgg
     1501 cccgcatccc ccgcgtgccg tcaccggctc cgggagagac ctacgccagg ctggagtctc
     1561 cccgcgggga gatcggatgc tacttggtga gcgacggggg tatgaagccc taccgcatga
     1621 agtggcgcgg agcctcgttc cacaacgtgg ccatgcttcc ggcgctgtcg gtgggataca
     1681 cgctctccga cctcatcaac atcgtgggga cgatcgaccc agtgatgggg gacgtggaca
     1741 gatgattgac agcgcccacc gatgacacgt gcgccgcagg cctatcttca cccgagcgct
     1801 ccgcgatgac ctggagcgac ctcgtcaatc cttgggtcgg catcgcgagg gggcacgcgc
     1861 gcaccctgaa gcagatggtc tcgcccccgg tcacccacat gtacccggat gagccggccg
     1921 aactctctcc ccgctggcgc ggtccgctcc gccttcgcgg agtcatggac gaccagcgac
     1981 ctcccgtcac cgacgcaccg ccgctcgctt tcaacgacct catgaccgca ctccacgacg
     2041 agcaacgtgt cgctccgtgc atgggcggct gccctgccaa cgtggatgcc cgcggtcaga
     2101 acgccctggt cgctgagggg agatacgcgg aggcgtatga cctcgtgcgc cgacggaacg
     2161 tcctgccggc cgtgctgggc tacgtctgca acaatccctg cgaggacgta tgtcgccgct
     2221 cctacctgga cgatcccatc gccatccgcc ggctgcatcg ggactgctac gaggtgtacg
     2281 accgtgaggt gcgcttcccc gggcatctgg cgagcctggt gcaccgcccc gaacacgtcg
     2341 ccgtcgtagg cgcgggtcct gccggcatcg cggcggccta cgacttagtc cagatgggct
     2401 accaagtcac ggtgtacgaa cgagaacccc ttcccggagg cctgctcgtc accagcgtcc
     2461 ccctctatcg actcgaccgt gaggtcgtga cgaaggagat cgctgatatc gccgagctcg
     2521 gagtggagtt ccggtgcggt gtgagcgtcg gagtcgatgt cacgctcgac cggcttcgag
     2581 gcgatcacga tgccgtcgtc gtagccgtgg gctactccgg tggacggatt ctccccctcc
     2641 cgggccacga cgccgaaggc gtgtggtcgg ccatagactt cctccacgcg tactgcatgg
     2701 gccagagccc gcgggtggga ccggaggtgg tcgtcatcgg tggtggcgac gtggggtgtg
     2761 actgctcgcg cagcgcgctc cgctgcggcg cgagcttgtc cgtgcaggca cacgtggaga
     2821 cgcgcgccga catgcccggc caggacgtcg aggtcctggg cgcgctcgag gagggcgtcg
     2881 ttcttctctc ggagtggggc ccggacgaga tcctggtgaa ggacggcaag gtggcgggca
     2941 tccgcctgaa gaaggtcgtg tcgcgcttcg accctgatgg aaggtggaac ccccagtact
     3001 ccgatgaggt tacggagatc cggtgccaga gcgtcatctt cgcagtgggc caagggctcc
     3061 agctcgactt cctgaagggg tcgggggtcg agttcgacga actcggccga cccctctccg
     3121 aaccgcacac cggagcgacc gccgtcgacg gactgttcct cgcgggcgac gtcgccaccg
     3181 gcccccgcac catcatcacg gcgatcggtc acgcgcacga gacggcactg tccgtgcacc
     3241 gtcacatcca gggcctgggt gtcgcggaag acagacgccc gcccctccac ccgccggagt
     3301 actacctgca aaagctctac gcgcccaacc cgccaggttt cgagcacgtc gggcctggcg
     3361 ggcggcgcgt gcgcatgccg gaatccgccc ccgaggaacg catccgcacc ggagcacagg
     3421 tggaactcgg atggccccgc ggcgacggac gccgcgaggc catccggtgc atgcgctgcc
     3481 agactcacgt gtgcgtggcc tgcacgttgt gcgcccgggt ctgcccggac aactgtatcg
     3541 acgtcgtagg ccacgacaca gggcacgaac gccgggtgga acgctacgac ttcgccatgg
     3601 aatggtgctg cttctgcggg ttctgccaag acgtgtgccc cacccaggcg ttgagcttgg
     3661 ccgccgactt cgactacgcc gcgcgcgagc gacgcgactt ctactacgac cgagccagga
     3721 tgctgcgccc ctttgccggc ccggacgaga cacccagcaa ggacggcctg ccgtgacgat
     3781 gaacggctgg gagtttggtt tgggcgggtg tcgtccggga gacaaccagt gcggttgggc
     3841 gtctgggggg gaaattccac tgtgattgga ccaacacgga gagcgccatg ctggcatcga
     3901 ccgagcgtct agacggtacg ccgttagtcg tcgtgaccgg ggagatcgac cacggcagtt
     3961 cgagcgccct ccaatcggag atagagccct gtctcgacgg tggagaaacg accatcctgc
     4021 tcgacctcaa ggatgtcacc tacatggatt ccggcggcat ctcggtgctt ctctacaccg
     4081 tgcggcggct gaggagaggg aatggctggt tgggtgtagt cagcccgaac acgaacgtgc
     4141 gcaggcttct gaagatcgtg ggactggagg tcgattccgg attccgagtg ttcgacgaca
     4201 aggagtcagc cgccaccgcg atccgggaga tgtcgacccc ttgaacgcgg gcggaacctc
     4261 gcggtgccgg atgatctcgg tagcacccga accgatcagg ctagccgacg tccggcgttt
     4321 cgtcgaagag accggcgctg aggcgcaact cgacacagac aagatcttcg acctcaaggt
     4381 agcggtgtca gaggcctgcg cgaacgcggt ggaacactcc ggcggccaac acgcccctct
     4441 gcaggtgtgc gccttcctct actcggacag gatcgtcttc gagatcagtg acggaggcga
     4501 cttccgcgtg cccggggttc cacaccggtc gccgcgtgac gaccgagggc tcggacttcc
     4561 gctcatggtc gcgctcatgg acgaggtgcg catagccaag acgcgcggag gcggcacgaa
     4621 cgtgcacctc tccctgttct tgtagccccc gcccgcgcag aacgctcagc cgcgcgctct
     4681 acctttcgcc gcgggccgcg gccggccctg cggcgtcggc cggatgcgct gcagccatgg
     4741 ccggcccgcg caccagggag accgccgccc ccaagaggca gattacgaac gcgatccaga
     4801 agacgtcgtg ggcggcatga aggaaggcct cccgcgcgga gagttcggga cccagtgcgg
     4861 ctatcgacgg ccgcagcagg tagccgggct cccggactgc gagcaccaac gccgccagcg
     4921 atacccccag gaccatcccg acgttccgcg ccatggcgac gacgcctgac gcgatgccca
     4981 accgttggcg cggaaccgag cccatcaacg cgctcgagtt gggcgattgg aacagggcca
     5041 tccccacgcc caccatcaga agtcgcagaa ggatacccac cggcccggtg tccacgccga
     5101 gtcgcgtgag gcccaccatc ccggccgcgc ccacgagcag ccccgtcgtg ctcaagaagc
     5161 gcgagccgat ccggtcggac agcgaacccg aaagcggccc caacaacgcc atggctgccg
     5221 ggatgggcgt gagtagaagc ccggcgtggg ccacggtgaa tcctctgatc tgaatgaagt
     5281 agaacggcat caggaagatc accgacgccg tcacgatgta gctggcgaga gcgctcgtgg
     5341 tcgatgcgct gaagaggcgg ttccggaaaa gggtgagatc caggagcgga tgcgcgaccc
     5401 ccgactcgac caccacgaac aggacggccc ccgccgcagc cacacccagc agcgccaaga
     5461 c
//
LOCUS       DASRMJ010000335         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_356668, whole genome shotgun sequence.
ACCESSION   DASRMJ010000335 DASRMJ010000000
VERSION     DASRMJ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356668"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFD74_09330"
     CDS             <1..949
                     /locus_tag="VFD74_09330"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592728.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HZK49782.1"
                     /translation="AAIMVTYPSTHGVFEDTITELCELVHEVGGQVYVDGANLNALIG
                     LAQPGKFGGDVSHLNLHKTFAIPHGGGGPGVGPVAVRNHLAPYLPNHPLAPEVGPATG
                     VGPVSGAPYGSASILPISWAYVRLMGGAGLTRATQVAILSANYIAARLREYYPVLYSG
                     NEGIVAHECILDLREITHVTGVSVNDVAKRLIDYGFHSPTMSFPVSGTLMVEPTESED
                     LVEIDRFCDAMIAIRGEIEQVARGEVSAADSVLHHAPHTAACLVEEWDHLYDRKLAAY
                     PAGVDKLSKYWPAVRRLEESYGDRNLVCSCPPIEVFEES"
     gene            1033..1458
                     /locus_tag="VFD74_09335"
     CDS             1033..1458
                     /locus_tag="VFD74_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZK49783.1"
                     /translation="MPLDSHRSVTITRVSTGRYIAVNGRGGVLDFGTGDVEDFTPVEL
                     FLTAIGGCTAVDVDILTTRRAEPESFVVQVDGEKVRDEGGSRMEELSVTFRVRFPEGE
                     GGDAARALLPDAMARSHDRLCTVSRTVELGTPVATRIES"
     gene            1468..>2100
                     /locus_tag="VFD74_09340"
     CDS             1468..>2100
                     /locus_tag="VFD74_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZK49784.1"
                     /translation="MNLSYRLLNVFTDGDNPFTGNPLCVFEDATGLSDQDMQDLARQL
                     NLSETTFVMPGGRNVSANVRIFTPNYEMPFAGHPTLGTAYVVHELFGGGDAILLRMPA
                     GDIPVRVSGNVWTLQANPPTSLPVDVPHSDLTAMIGLTADRVAAEPLWVDTGTVQLIL
                     PLRSAEDVRAAAPDPRLLVQFATRPGGESLVYLWAPTGPETIEARLFFTQN"
BASE COUNT          342 a          723 c          672 g          363 t
ORIGIN      
        1 cgcggcgatc atggtgacct acccctcgac gcacggtgtc ttcgaggaca cgatcaccga
       61 gctgtgcgag ctcgtccatg aggtcggagg ccaggtctac gtcgacggcg ccaacctcaa
      121 cgcgttgatc ggcctcgcgc agccgggcaa gttcggcggc gacgtgtcac acctgaacct
      181 gcacaagacg ttcgccatcc cgcacggtgg cggcggcccc ggagtcggtc cggtggccgt
      241 gcgcaaccac ctggcgccgt atcttccgaa ccacccgctg gcccccgaag tcggccccgc
      301 cacaggcgtc ggcccggtct cgggcgctcc ctacggctca gcgagcatcc tgccgatctc
      361 gtgggcctac gtccggctca tgggcggcgc cgggctgacc cgtgccaccc aggttgccat
      421 cttgtcggcc aactacatcg ccgctcgact gcgtgagtac tacccggtgc tgtactccgg
      481 gaacgaaggg atcgtggccc acgagtgcat ccttgacctg cgcgagatca cgcacgtcac
      541 gggagtttcc gtgaacgatg tcgccaagcg cctcatcgac tacgggttcc actcaccgac
      601 gatgtccttc cccgtctccg gaaccctcat ggtggagccg accgagagcg aagacctcgt
      661 cgagatcgac cgcttctgcg acgccatgat cgccatccgc ggtgagatcg agcaggtggc
      721 ccgcggcgag gtctcggcag cggacagtgt gttgcaccat gccccgcaca cggcggcctg
      781 cctggtcgag gaatgggacc acctctacga ccgcaagctc gcggcctacc cggcgggcgt
      841 ggacaagctc tcgaagtact ggccggcggt acgtcgcctc gaggagtcgt acggcgatcg
      901 caacctggtc tgttcctgcc cgccgatcga ggtcttcgaa gagagctgag actccgacct
      961 tcagcccgta tgccgccgcc accctccgag ggggcggcgg catctcgctg ggcactctgg
     1021 catggtgagg gcatgcctct tgactcacat cggtcggtca ctatcactcg cgtttcgacc
     1081 ggccgctata tcgcggtcaa cggtcgaggc ggcgtactcg acttcggcac tggggatgtg
     1141 gaggacttca cgccggtgga gctgttcctc accgccattg gcgggtgcac cgcggtcgac
     1201 gtcgatatcc tcacgacccg ccgtgccgag cccgagtcgt tcgtcgtgca ggtcgacggc
     1261 gagaaggtac gcgacgaagg aggcagccgg atggaggagc tctcggtgac cttcagggtg
     1321 aggttccccg agggtgaggg tggagacgcc gctcgagctc tgcttcccga tgccatggcg
     1381 cgttcccacg accggctctg cacggtcagc cggaccgtgg agctcggcac ccctgtggca
     1441 acccgtatcg agtcctgatc cgctgtcatg aacctgagct accgcctgct caatgtcttc
     1501 accgatggcg acaacccgtt caccggcaac ccactatgcg tgttcgagga tgcgaccggc
     1561 ctctctgacc aggacatgca agacctggcg cgtcagctga acctctccga gacgaccttc
     1621 gtgatgccgg gaggccggaa cgtcagcgcc aacgtccgca tcttcacgcc caactacgag
     1681 atgccgttcg cgggacaccc gacgctgggc acggcatacg tcgtgcatga gctgttcggt
     1741 gggggagacg caatcctcct tcgtatgccg gccggagaca tcccggtgcg ggtctccgga
     1801 aatgtgtgga ccttgcaggc gaacccgccg acgtccctcc ctgtcgacgt gccgcactcg
     1861 gatctgaccg caatgatcgg gctgaccgcg gatcgcgttg cggccgaacc attgtgggtc
     1921 gacacgggca cggtccagct catcctgccg ctgagatcgg ccgaggacgt cagggccgcg
     1981 gcgcccgatc cgcgcctctt ggtccagttc gccacgaggc ccggcggtga gtcgctggta
     2041 tacctctggg cgccgacggg cccggagacg atcgaggcgc gcctcttctt cacccagaac
//
LOCUS       DASRMJ010000336         7969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_503909, whole genome shotgun sequence.
ACCESSION   DASRMJ010000336 DASRMJ010000000
VERSION     DASRMJ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503909"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1927)
                     /locus_tag="VFD74_09345"
     CDS             complement(<1..1927)
                     /locus_tag="VFD74_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZK49785.1"
                     /translation="MSRCVWFSAAGGLALAGLAQPAAAPLVGAAGVVFCLLGFAAFRS
                     GGRGAGAGICGHCVPFLLVVLSLLVGASWGAVRVGELLHGDLAGRVGERCEIVVEVSE
                     QPRARGQGASFAAVSLGPVGSGERLLVEIARAGPGLGGSPELREGSVLRVRGSFRTPE
                     TPKAPSGSAFDERAWLAREGIGVVLSADSAGVALLGHRAGLAGVLDGLRRRARSHMSV
                     GIDPVTGSMLKGILLGDKADIPETMLQAFRRAGTAHMLAVSGLHVGALAGFVMAGLQA
                     ARVSPGGRTVAGSAVVVLFALLTGAGPSVTRAAVMVIVVLAARFAGRGRDTWSALGLA
                     AVVVLAREPGAVAGPGFQLSFSAVAALLCLTRPLESRLRARLPEGLATAAAVSLAAGI
                     GTAPVSLLTFGQVSLVGVFANPLAVPVVPLIMALGFGSVAAGFLSLSVSGALNTVSGV
                     LVGWVSAVSTLMAKAPVMTWANAASVIMGVSGALLCGRMAARYRGSRGSRMMKAGSLH
                     PAARVMAGLAGALLLGVLPLGTSAAASRATVWWAARDWPTVGEARVIDVGEGSALLVR
                     SPDGVSVLVDGGPAGSGLLGDLQRLGVSRLDVLVVSHPHADHFAGLAEVVERMPVGLF
                     VDHVEEVSPPGGVAAGMG"
     gene            complement(1981..2520)
                     /locus_tag="VFD74_09350"
     CDS             complement(1981..2520)
                     /locus_tag="VFD74_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HZK49786.1"
                     /translation="MSVPTGWEDPGSIAGPTGTPPESTSTTAAPTLFVQVAGAVQRPG
                     VYEVEAGARVFQVLLMAGGMTAEADGEAVPLAAEVADGACIRVPRVGETLSGPVLQVP
                     PAAGGAGQSGTVPVGPISLSTATEAELDTLPGVGPKTAQRIIAYRVENGPFLSVDQLE
                     EVSGIGPATLENLRDLVVP"
     gene            complement(2883..3455)
                     /locus_tag="VFD74_09355"
     CDS             complement(2883..3455)
                     /locus_tag="VFD74_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZK49787.1"
                     /translation="MELKGSRTEQNLMKAFAGESQARNRYTYASAAARKEGYVQIGNI
                     FLETAENEREHAKVFFKLLEGGTVEFTASYPAGPTGETAANLLAAAEGEKEEWGTLYP
                     EFAQVAEDEGFKKVAARFRNIAEVESYHERRFRKLLANVEAGTAFSRSEDVRWKCNNC
                     GYVHSGPGAPKVCPACEHPQAHFELWVEAY"
     gene            complement(3479..5767)
                     /locus_tag="VFD74_09360"
     CDS             complement(3479..5767)
                     /locus_tag="VFD74_09360"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK49788.1"
                     /translation="MADFESFGLSGPVLEAINKMGFVEATPIQKKAIPIIMQGRDLLG
                     QAQTGTGKTAAFGIPIAERLPADSPAVQALVLSPTRELAVQIAEELHELTLFSGHKVL
                     PVYGGQRIDRQITALRRGVQVVVGTPGRILDHLGRGTLSLSEIKMLVLDEADMMLDMG
                     FIPDIRRILRQCPKERQTLLFSATIPSDIERIAREHMKDPEKVSVVPETLTLEGTEQI
                     FYEVPEDEKIDALMRLLDFEEEGASAIIFCRTKRNVDRLARKLKARGYDAEGLHGDLT
                     QAQRDQIMQGFRDQRFAYLVATDVASRGLDISHVTHVINFHVPQDPESYVHRIGRTGR
                     MGRSGVAITFVTPAEYWDLLRIQEFSMATIEEGELPTSAEVDERRRASRGDAKARRRV
                     AEADRVVGRGARLADDEAGGDLDLVEETPVPDVEEAAPAPKRRRTRKKPEELPLSGIL
                     QQREEPTTEAGRDQLRYALRDARKRIGWVDESEALKEASRIEHEQRVDVAHAAALEGE
                     GDGGGEGPDADQVSARLGAEEARRADVLARLQAAEAAIEMEGQERRDAGIPEGVPAGA
                     APPEQAAAAGEGPLIAPDQEARILRAMRLRDRVSRLVAGLDLNDLADFEAILSRLEYD
                     HELRHITAALIKALAGQDEPALGQATALEPPFEPKVSVSAPSSGADDEGRGARADEMT
                     RLFISIGRRAHVNRESLERLVRETAQIDGDDIGRIDLLHNFAFIEVRKTVASRVIEGM
                     HETMFKGREISVEPAKSSAKEE"
     gene            complement(5860..6666)
                     /locus_tag="VFD74_09365"
     CDS             complement(5860..6666)
                     /locus_tag="VFD74_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZK49789.1"
                     /translation="MPFHDALGLFYTDEGSGPPVVFVHGLGLGHGLWAEQAPLAAEFR
                     VITYDARGCGTSRVPPTGYDYPDLSRELLGLLDLLEIDKAHLVGHSRGGGVIMETGLQ
                     NPERVASLFFVDSVLRGFPWSDEFTSTMRRAAEVAQQSGPRAAFDQAWTEAGIFSWVR
                     TRRPEVYQRLLELCEGWSGAEWLDTARYPKQAVSDIDRLADISIPTFVLSGQEDMHDF
                     VEIANMLAWWIPGAIQKSLVGVGHFPMLENPHETNLYLRSFLRRVVGLRE"
     gene            complement(6677..7387)
                     /locus_tag="VFD74_09370"
     CDS             complement(6677..7387)
                     /locus_tag="VFD74_09370"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HZK49790.1"
                     /translation="MTRLPDHGPAAPPPPAGVPRREVTIPGDVSLEGLLEFPEGGAPV
                     GGVVVAHPHPLHGSTLHQPVVHHVAKACRAHGLATLRFNFRGVGASGGVYSGLEEFRD
                     VRAATAFLRAELPADAPVSLAGYSFGAVMASLAVLDGEPAAALALVAFPLRWDEFVPG
                     WFDRFGEYGGPVIAVCGERDIVAPPAEVEAFLRGVGLDPTMVVVPGADHFFAGASAGQ
                     RDRFSEPTAEFLANASRA"
     gene            complement(7390..7881)
                     /locus_tag="VFD74_09375"
     CDS             complement(7390..7881)
                     /locus_tag="VFD74_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZK49791.1"
                     /translation="MPSFDVVSRVDMQEMDNTVNMLKKEVTARYDFRGSKTEVELDKK
                     ESLIRLLTEDDMKLRALRDVLIAKAVKRGIDTKAFEFGDPERAGGDMLRQEVKIANGI
                     DIETARKVAKLVKEAKLKVQAAIQGDEVRITGKKRDDLQDAIALLKEADLGVPLQYVN
                     MRE"
BASE COUNT         1303 a         2828 c         2580 g         1258 t
ORIGIN      
        1 cccccattcc agctgcgacc ccgccggggg gcgaaacctc ctccacgtgg tccacgaaga
       61 gccccaccgg catgcgttcc accacttcgg ccaatcctgc gaagtgatcg gcgtggggat
      121 ggctgaccac cagcacgtcc aggcggctca ccccaagtcg ttggaggtca cccagcagac
      181 cggatccggc cgggccgccg tcgacgagca cggagacccc gtcaggcgag cgcacgagca
      241 gggccgagcc ttcccctacg tcgatcaccc gagcctcgcc gacagtaggc cagtcccgcg
      301 ctgcccacca cacagtggcc cgcgaagccg ccgccgaggt acccagcggc agcacaccca
      361 ggagcaacgc tcccgcaaga cccgccatga cccgcgccgc cggatgcaga ctcccggcct
      421 tcatcatccg cgacccgcga ctcccccgat accgtgctgc catccgcccg cacaggaggg
      481 cgccgctcac acccatgatc acggaggcgg cattcgccca cgtcatcacg ggtgctttcg
      541 ccatcagcgt ggagacggcc gacacccagc ccacgaggac ccccgaaacc gtgttcagcg
      601 ctccggagac actaaggctc aagaagcctg cggccacgct tccgaaaccg agtgccatga
      661 tcagcgggac caccggcacg gccagcggat tcgcgaacac tcccacgagg gacacctgtc
      721 cgaacgtgag cagggagacg ggtgccgtcc ctatcccggc ggccaacgac acggccgccg
      781 ccgtcgccag gccttcgggg aggcgcgcac ggagacggga ctcgaggggg cgcgtgagac
      841 acaggagagc cgccaccgcg ctgaacgaga gctggaagcc gggcccggcc actgcaccgg
      901 gctcgcgcgc cagcaccacc accgccgcca gccccagagc ggaccaggtg tcgcgtccgc
      961 ggcccgcgaa acgggccgcc aggaccacga tcaccatgac ggcggcccgc gtcaccgagg
     1021 ggccggcccc ggtgagcagg gcgaagagca ccaccaccgc ggacccggcc acggtcctgc
     1081 ccccgggaga gacgcgcgcc gcctggagtc cggccatgac gaaccccgcg agggcgccca
     1141 cgtgtagtcc gctgacggcc aacatgtgcg cggtgcccgc gcgtcggaac gcttggagca
     1201 tggtctccgg gatgtccgcc ttgtcgccca ggaggatccc cttgagcatc gagccggtca
     1261 cagggtcgat gcccaccgac atgtggctgc gggcccgcct acgaagtccg tccaacactc
     1321 cggcgagtcc tgccctatga ccgagcagtg cgacgccggc cgaatcagcc gacaggacga
     1381 cgccgatgcc ctcccgcgcc agccatgctc gttcgtcgaa cgcggatccc gagggggcct
     1441 tgggcgtttc gggtgtccgg aagctgccgc gtacgcgcag gacgctccct tcccgcagct
     1501 cgggcgatcc gcccaatccg ggccccgccc gggcgatctc cacgagtaga cgctccccgc
     1561 tcccgacggg accaagggac acagccgcga acgaagcgcc ctgaccccgg gcgcgtggct
     1621 gctccgacac ctcgaccacg atctcgcacc gttctcccac ccggcccgcc aggtctccgt
     1681 gaagcagttc gccaacgcgg accgcccccc acgaagcgcc caccagcagc gacagtacga
     1741 ccagcaggaa gggcacgcag tgcccacaga tacccgcgcc ggcgcctcgc ccgcccgacc
     1801 ggaacgccgc gaacccgaga aggcagaaga ccaccccggc ggcgcccacc agtggtgcgg
     1861 ccgccggttg cgccagcccc gcaagggcga gcccgcccgc ggctgagaac cacacgcatc
     1921 gcgacacccc cgctcgtcga accatccagc gggagaaacg ctcgagcgcc gcggttacgc
     1981 tcacgggacg acgagatcgc gcaggttctc caatgtggcc gggccgattc ccgagacctc
     2041 ctccagttgg tcgacggaga ggaacggacc gttctcgacc ctgtacgcga tgatgcgctg
     2101 cgccgtcttc ggcccgacgc caggcaaggt gtcgagctcc gcttcggtgg cggtgctgag
     2161 tgagatgggg ccgacgggca cggtgccgga ctgccccgcc ccgccggccg ccggaggcac
     2221 ttgcaggacg ggaccggaca gcgtctctcc caccctcggc acccggatgc acgcaccgtc
     2281 ggccacctcg gccgccaaag gcactgcctc cccgtcggcc tccgcagtca tgcccccggc
     2341 catgagcaac acctggaaca cgcgcgcccc ggcctccact tcgtacaccc cggggcgctg
     2401 cacggccccg gcgacctgca cgaacagggt cggcgcagcc gtggtggagg tggactccgg
     2461 cggcgtcccg gtggggcctg ctatggaccc cggatcttcc cagccggtcg gaacggacac
     2521 gccgggcgcg ccagggtccg ccgtccaggt gccgccggga gcgtggcccc ccgcactcga
     2581 agcacgcatg ctccaggctc cgacagccac gacgacaatt gcggcgacgg cgtacgggac
     2641 gatcctcttt cgcgacaaga cgggcatggc ggcagagtag tcggcgcgtg ggccgccgcg
     2701 aatgggacct tgggctcaga ctgggctggg tcccgcgaat gggaccttgg cctcagactg
     2761 gctggggagc cggagaggag cggagacgcg accggcggcc ccgcgaccct cagggtgagg
     2821 gctcggggtc gccggcgact atggcatcgt ggatcctggg aaccgagagg aagctctcgc
     2881 tgctagtagg cttccaccca gagttcgaag tgggcctgag gatgttcgca ggcgggacag
     2941 accttgggcg cgccgggacc ggagtgcacg tatccgcagt tgttgcactt ccagcgcacg
     3001 tcttcagagc gcgagaacgc ggttccggct tccacattgg cgagcaactt gcggaagcgc
     3061 cgctcgtggt agctctcgac ctctgcgatg ttgcggaagc gggcagcaac cttcttgaag
     3121 ccctcgtcct cagccacctg ggcgaactcc gggtagagcg tgccccactc ctccttctcg
     3181 ccctcggcgg cggccagcag gttcgcagcc gtttcgccgg tggggcccgc cggataggag
     3241 gccgtgaact ccaccgtgcc cccttcgagg agcttgaaga acaccttggc gtgttccctc
     3301 tcgttctcgg cggtctccag gaagatgttg cctatctgga cgtagccttc cttgcgtgcg
     3361 gcagcagaag cgtacgtgta gcggttgcgg gcctgagatt cgcccgcgaa cgccttcatc
     3421 aggttctgct ccgtccgtga tcccttcagc tccatgcggt ttcctccctt tcgcgcgcct
     3481 actcttcttt ggcgctggac ttcgccggtt ctaccgagat ctctcggccc ttgaacatgg
     3541 tctcgtgcat gccttcgatc acccggctcg caacagtctt ccgcacttcg atgaacgcga
     3601 aattgtgcag cagatcgatg cggccgatgt catctccgtc gatctgcgcc gtctcgcgga
     3661 ccagacgctc gagcgactcc cggttgacgt gggcgcggcg gccgatggag atgaacaacc
     3721 gcgtcatctc gtcggcccga gccccgcggc cctcgtcgtc ggcgccggag gacggtgcgg
     3781 agacggacac cttcggctcg aagggcggct cgagtgcagt cgcctgcccg agtgccggct
     3841 cgtcttgccc ggccagagcc ttgatgagcg ccgcggtgat gtggcgaagc tcgtggtcgt
     3901 actcgagtct ggacaaaatg gcctcgaagt cggccagatc gttgaggtcg agcccggcga
     3961 ccagtcgcga aacgcggtca cgcagacgca tggcccgcaa gatgcgcgcc tcttggtcgg
     4021 gcgcgatcag cggaccctcg ccggcagccg cggcctgttc cggaggtgcg gccccggcag
     4081 gcacgccctc ggggatcccg gcgtcccgcc tctcttggcc ctccatctcg atggccgctt
     4141 cggccgcctg gaggcgggcg agtacatcgg ccctgcgcgc ttcttcggcg ccgaggcggg
     4201 cggagacctg gtcggcatcc ggcccctcgc cgcccccatc gccctcgcct tcgagtgcgg
     4261 cggcatgtgc cacgtccacc cgctgctcgt gctcgatgcg ggatgcctcc ttgagcgcct
     4321 cgctctcgtc gacccagcct atgcgcttgc gtgcgtcgcg caacgcgtat cggagttggt
     4381 cgcgacccgc ctcggtggtg ggctcctccc gctgctgcag gatcccggag agtggcaact
     4441 cctcaggctt cttgcgcgtg cgacgccgct tgggagcggg cgccgcttcc tcaacgtcgg
     4501 ggacgggtgt ctcttcgacc aggtcgaggt ctccacccgc ttcgtcgtcg gcgagacggg
     4561 ctccgcgccc gacgacgcga tccgcctcgg ccactcggcg acgggccttc gcatcgccgc
     4621 gggaggcgcg tctgcgctca tcgacctcgg ccgatgtggg aagctccccc tcctcgatgg
     4681 tggccatcga gaactcctgg atacgcaaga gatcccaata ctcggccggc gtcacgaaag
     4741 ttatggccac cccggagcga cccatgcgac cggtgcggcc gatgcggtgg acgtacgact
     4801 cggggtcttg gggcacgtgg aagttgatga cgtgggtgac gtggctgatg tcgaggccgc
     4861 gggaggcgac atcggtggcc accaagtagg cgaagcgttg gtcgcggaag ccctgcatga
     4921 tctgatcgcg ctgcgcttgg gtgaggtccc cgtggagtcc ctcggcgtcg tacccccgcg
     4981 ccttaagctt ccgcgcgagc ctgtccacgt tccgcttggt gcggcagaag atgatcgcgc
     5041 ttgccccttc ttcttcgaag tcgaggagac gcatgagggc gtcgatcttc tcgtcctcgg
     5101 ggacctcgta gaatatctgc tcggtgccct cgagcgtcag cgtctcgggg accaccgaca
     5161 ccttctccgg gtccttcatg tgctcacggg cgatccgctc gatgtcggac gggatggtgg
     5221 cggagaacag gagggtctgc cgctccttcg gacactgacg gaggatgcgg cggatatctg
     5281 ggatgaaacc catatccagc atcatgtccg cttcgtcgag caccagcatc ttgatctcgg
     5341 aaagggagag agtcccccgc cccaggtgat cgagtatgcg gccgggagtg cctacgacca
     5401 cctgcacgcc tcgcctgagt gccgtgatct gccggtcgat ccgttgccct ccatacacgg
     5461 ggagcacctt gtgcccgctg aagagggtga gttcgtgcag ctcctcggcg atctgcaccg
     5521 ccaactcgcg ggtcggagaa aggaccaaag cctgcaccgc cggggagtcc gccgggagcc
     5581 gctctgcgat ggggataccg aaagcggccg tcttgccggt tcctgtttga gcctggccga
     5641 ggaggtcacg gccctgcatg atgatcggga tcgccttctt ctggatgggt gtggcctcca
     5701 cgaagcccat cttgttgatt gcttccaaca ctggtccgct aagaccaaaa ctctcgaaat
     5761 cagccatgtg ctgtcacgtt tcctggggta gacatgatcc ggaagcccgg agggccacgg
     5821 gctcgatacc agggagacta tagcccaaag aggccttact cactcgcgca gacccacgac
     5881 gcgccgcagg aagcttcgca ggtagagatt ggtctcgtgt ggattctcga gcatggggaa
     5941 gtgcccgacg cccacgagcg acttctggat ggctcccggg atccaccacg cgagcatgtt
     6001 cgcgatctcg acgaagtcgt gcatgtcttc ctgcccggaa agcacgaagg tgggaatcga
     6061 gatgtccgcg agacgatcga tgtccgacac cgcctgcttc ggatagcgcg cggtatcgag
     6121 ccactcggct cccgaccacc cttcgcacag ctccagcagg cgctggtaca cctccggacg
     6181 cctggtcctg acccaactga agatgccggc ctccgtccac gcctggtcga acgccgcccg
     6241 cggtccgctc tgctgtgcca cttcggctgc gcggcgcatg gtggatgtga actcgtcgga
     6301 ccaggggaat ccccgcagca cgctgtcgac gaagaagagg gaagcgacac gttcgggatt
     6361 ctgcaggccc gtctccatga tcacgccgcc gcctcgggaa tgacccacca ggtgcgcctt
     6421 gtcgatctcc agcagatcga gcagccccag cagttctctt gagagatcgg gatagtcgta
     6481 gcccgtcgga ggcacccggg acgtgccgca ccctctcgcg tcgtaggtga tcacgcggaa
     6541 ctccgcggca agcggggctt gttcggccca gaggccgtgc ccaagcccca agccgtggac
     6601 gaagaccacg ggtggtccgc ttccctcgtc ggtgtagaag agccccagtg catcatggaa
     6661 cggcatgtgt cctcccttac gcccgcgagg cgttcgctag aaactccgcc gtcggctcgc
     6721 tgaagcggtc tcgttgcccg gccgaagcac cggcgaagaa gtggtcggct ccaggcacga
     6781 ccaccatggt ggggtcgagg ccgactccgc gaaggaaggc ttctacctcg gccggagggg
     6841 cgacgatgtc acgctcgccg cacaccgcga tcaccggtcc accgtactcc ccgaaacgat
     6901 cgaaccaacc gggcacgaac tcgtcccacc gcaacgggaa cgcgaccagg gcgagcgccg
     6961 cggctggctc gccgtcgagc actgccagcg acgccatcac cgccccgaag gagtagcccg
     7021 ccagcgagac aggcgcgtcc gccggcagtt ccgccctcag gaaggcggtg gcagccctca
     7081 cgtcacggaa ctcctccagg ccgctgtaga cgccgccgct ggctccgacg ccgcggaagt
     7141 tgaagcggag ggtggccagg ccgtgggcgc gacaggcctt ggcaacatgg tgcacaacgg
     7201 gctgatgcag ggtgcttcca tggagagggt gagggtgggc caccactacc ccgcccaccg
     7261 gcgcgccacc ctcggggaac tccagcagtc cctcgagcga cacatcgccg gggatcgtga
     7321 cctcccggcg gggcacacct gccggaggtg gcggagcagc aggtccgtgg tccgggagtc
     7381 gggtcaccgc tactccctca tgttgacgta ctgcagcggg actccgagat cggcttcctt
     7441 gagcagcgcg atggcgtcct gcaggtcgtc gcgcttcttc ccggtgatgc gcacttcgtc
     7501 gccctggatc gcggcctgca ccttcagctt ggcctctttc accagcttgg ccaccttccg
     7561 cgcagtctcg atgtcgatgc cgttcgcgat cttgacttcc tgacggagca tgtcaccccc
     7621 ggcccgctcg ggatcgccga actcgaaggc cttggtgtcg atgccgcgtt tcaccgcttt
     7681 cgcgatcaga acatcgcgaa gggcacgaag cttcatgtcg tcctcggtga ggaggcggat
     7741 gaggctctct ttcttgtcga gttccacctc cgtcttcgac ccacggaagt cgtagcgggc
     7801 agtcacttcc ttcttgagca tgttgaccgt gttgtccatc tcctgcatgt ccacgcggct
     7861 gacgacgtcg aaagacggca tgactcctca ctctcctcgg gtgccccgcc ggtgcttcgg
     7921 cggcgccagg tgatcctctc tcgatattac caggcccccc gcggcgggc
//
LOCUS       DASRMJ010000337        18801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_71623, whole genome shotgun sequence.
ACCESSION   DASRMJ010000337 DASRMJ010000000
VERSION     DASRMJ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 18801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18801
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71623"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1663)
                     /locus_tag="VFD74_09380"
     CDS             complement(38..1663)
                     /locus_tag="VFD74_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011174141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZK49792.1"
                     /translation="MAQAWGYAVVGTAAVPVRVEAHVRPGLPGMTVVGLPGAAVREAR
                     ERVRSGASSSGLPLPTQRITVNLSPADVRKESPGFDLPIALAVLAASGHLPVDTLKRA
                     GAVGELALDGGVRPVKGMLPVAEAARDDGRDVLLVPVAALPEVWAVEGIGFIGVASLR
                     EAVLALRDPRCAARLCERGRRWVARRNRSAPPMSQCPDLIDVVGHARARRALEIAAAG
                     GHHALLVGTPGAGKTMLARRVPSILAPLSREESLEVTRIWSVAGLHRPELGLVQTRPF
                     RSPHHSASRAAVIGGGASPGPGEASLAHRGVLFLDELPEFARDVLESLRQPLEEGDVT
                     ISRAGGSIRLPARFALVAAMNPCPCGYSGHPERECRCTAEQIARYSGRLSGPLLDRID
                     VFVEVEPLTREDLDSMPSPPENSATVRARVIAAQSFRRERVGAEPHGASVGTATAHDG
                     PPQSSAAGEDPSPVASREHPLRLEAESRLDRDARRLVRGALARGLLGGRGFVRTLAVA
                     RTLADLDASLDVGSGHVAEALTLRQIPWSWEGG"
     gene            complement(1679..2047)
                     /locus_tag="VFD74_09385"
     CDS             complement(1679..2047)
                     /locus_tag="VFD74_09385"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZK49793.1"
                     /translation="MTAVERGRNAEDAAGEYLVSRGWELVARNHRTPVGEIDLVCRDD
                     ATLVIVEVKARSGSYFGEALESIGPRKERRLRAAAAWWMAERGRVTRNVRFDVVVVML
                     EDDGSLRSLAHLRDVLGPGR"
     gene            complement(2141..2689)
                     /locus_tag="VFD74_09390"
     CDS             complement(2141..2689)
                     /locus_tag="VFD74_09390"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZK49794.1"
                     /translation="MVAAVSLDYRRPLAGVLRGLTDSKVLSREARESLYPRILVAASE
                     VVIVAVSPVTIDRVGLHRCNLEALKTALYRLDNHYDIAFVDGFALDDERLRATQLIRG
                     DAKSASVAAASVIAKVTRDRLMARLNETYPGYGFLQHVGYGTQAHRAALRELGPCEIH
                     RHSFAGVGFPQLALFSGTPISD"
     gene            complement(2851..3408)
                     /gene="lepB"
                     /locus_tag="VFD74_09395"
     CDS             complement(2851..3408)
                     /gene="lepB"
                     /locus_tag="VFD74_09395"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZK49795.1"
                     /translation="MLKRLRRLPSGDQAGSSLLGSLLEVVFIVGAAFVLALLIQQFVV
                     KPFYIPSESMEPTLVKGDRVLVNRFVYRFNDPKPGDVIVFHPPTAPEQDYIKRVVAVG
                     GDTVEVKGGLLFVNGVAQDEPYLNERNIEGVFPSETVPEGYVFAMGDNRNRSSDSRVF
                     GPVKLESILGKGFVIYWPPGKIGWL"
     gene            complement(3422..3769)
                     /gene="rplS"
                     /locus_tag="VFD74_09400"
     CDS             complement(3422..3769)
                     /gene="rplS"
                     /locus_tag="VFD74_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZK49796.1"
                     /translation="MSVIIQALEQQQLRDDVPRFKAGDTVKVHFKVIEGVRERIQVFQ
                     GICIKRQGEGVRETFTVRKQSFGVGVERTFPVHSPKIDKLEVLAIGDVHRAKLYYLRD
                     KIGKKARVKEKRV"
     gene            complement(3836..4585)
                     /gene="trmD"
                     /locus_tag="VFD74_09405"
     CDS             complement(3836..4585)
                     /gene="trmD"
                     /locus_tag="VFD74_09405"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZK49797.1"
                     /translation="MKRIDILTLVPEAFGWFLHQHPIADALSRGCVDVRIHNIRDYTP
                     LNHRQVDDTPYGGGPGMVIRVDVMAAALTGVFGVEAHRVQEERDVVLLAPAGEPFDDP
                     TAAALARDCRDLVLLCGRYEGFDARVGELFASAEVSVGPYVLAGGEVAAFAVVEAVVR
                     KIPGVLGNEESLAEESFSEGLGGAGEYPQYTRPRQFMGSEVPEILVGGNHAAIDRWRR
                     ERARPSRWPAWTERQRTPRTAAEVEFGRAME"
     gene            complement(4660..5208)
                     /gene="rimM"
                     /locus_tag="VFD74_09410"
     CDS             complement(4660..5208)
                     /gene="rimM"
                     /locus_tag="VFD74_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZK49798.1"
                     /translation="MSLPEWIEVGRISRAHGVRGDVRVTLSTDNPDRFVKGAVLHGVP
                     SRPGLRQAESGRVTLEIESIKGTADSPIVKFAGVDDRTQAEGFRGWVLQVPLSELPAL
                     EDGEFYSFELEGLEARSLDGTVVGVVREVLESPAHDLLAIELSSGGEMLAPFIHEFVP
                     VVEVESGYVTVDVERLRPADDD"
     gene            complement(5237..5467)
                     /locus_tag="VFD74_09415"
     CDS             complement(5237..5467)
                     /locus_tag="VFD74_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013507148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZK49799.1"
                     /translation="MKNLLEYLAKALVDSPDEVRVESTETETTVVLELNVAREDIGKV
                     IGKQGRIARALRTVVKASAVKNGKRAIVEIVD"
     gene            complement(5570..5812)
                     /gene="rpsP"
                     /locus_tag="VFD74_09420"
     CDS             complement(5570..5812)
                     /gene="rpsP"
                     /locus_tag="VFD74_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZK49800.1"
                     /translation="MATAIRLTRRGSKKHPMYRIVVADSRAPRDGRFIENIGTYNPQT
                     EPSTVEVDADRAKDWLSKGAQPSGAVRKLLEIKGIL"
     gene            complement(5868..7196)
                     /gene="ffh"
                     /locus_tag="VFD74_09425"
     CDS             complement(5868..7196)
                     /gene="ffh"
                     /locus_tag="VFD74_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZK49801.1"
                     /translation="MFDSLSDKLQGALDGLKGQGKLSEKDVERAAREIRLALLEADVN
                     FKVVKDFVSRVKERALGAEVLESLTPAHQFVKIVSDELTTLMGSTSTRLTFSPRPPTV
                     VMMVGLQGSGKTTACGKLAAFLSKQGKSPALVAGDVYRPAAVEQLKILGEQLGVPVYD
                     EGTTSDPVDIAVNGLKWARESGRDVVIIDTAGRLHIDERLMDELVGIRSRVKPHNILL
                     VLDAMTGQDAVNVAQQFQEKVQFDGVILTKLDGDARGGAALSVRAVTGRPIKFASTGE
                     KLDSFDYFHPDRMASRILGMGDVLSLVERAEAAVDEKKARELEKRLRKSQFTFEDFLE
                     QLEQVRKMGSLQSILGMIPGLSAGKLKGLQVDDTAFDRIQAIIHSMTLEERRHPETIN
                     GGRRRRIASGSGNDIQAVNQLLNQFKQMQKMMKQLGSGKMPKNPFQTLGM"
     gene            complement(7380..8390)
                     /gene="ftsY"
                     /locus_tag="VFD74_09430"
     CDS             complement(7380..8390)
                     /gene="ftsY"
                     /locus_tag="VFD74_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZK49802.1"
                     /translation="MSYNWSEVFIGVDARATVGPPDAKLERKRGWFRQLREGLIKSRQ
                     ALQQQFSAIITDRFDEDLWERIEEALIYADVGVDTTISIVRRLEETAEAGGIPDGARL
                     LAELRRISAEHFCSGDTRIDVSAQPAVLLMAGVNGTGKTTTIGKIAWHLKELGKEPLL
                     VAGDTFRAAAAEQLVEWGRRVGCEVVRHDKGGDPGAVAYDGIEAAKARGVDVVIIDTA
                     GRLHTQVNLMRELEKVVRVVKKQVPDAPHETLLTIDATTGQNGLIQARLFKEVIDVSG
                     VVLTKMDGTAKGGIALAISHELDVPIKLIGVGETMDALQPFDAAGFTEALFGDELLRG
                     RA"
     gene            complement(8472..11981)
                     /locus_tag="VFD74_09435"
     CDS             complement(8472..11981)
                     /locus_tag="VFD74_09435"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK49803.1"
                     /translation="MTFLSRIRMKGFKTFARPTELVFEQGVSVIIGPNGSGKSNVADA
                     VLWALGEQSPTTIRARSMQDVIFSGSDGQRPAAVAEVSLVFDNAKGVFPLDFAEVEIS
                     RRVTRDGTSKYRINGAASRLVDVEDLVGGVGLGRYKKRRDRALAKLEKVRTNLLRVSD
                     VEREVRAALRPLKQQVSAAERYAEASEEVARLRAQGALLALVGLEGRIETERSTFDEV
                     SARRAETEASLAALRDERTREEERFTTALREREALSAVYHQAAADVDRVRSRGASLDQ
                     RVARMEAELARARRRAAIAAEEDGVARARVDALGDGPAQAAGRLNLVVSAAESLAARL
                     EEMRPSLEAVARDEDVLRDEVFGLEAERARALQEKDFLVREMAGRTRRAEELAEAVRV
                     AARRLDEVRNTSRERAGMVAAAEVTLAGLEEEVSVARRGAAALQMEADQARVLARSLA
                     ERLSAATSRVTVLEEVASRREGTPPAARALLESESGAVLVVEALRVRAGYERAVAAAL
                     GPLASAVVVSGGTDAELLRSGEGLVEILWPAGEPRSDPDGGKAQAPAAPAAPDARTGG
                     VDIWDVIDGPEGVVRALKAILPPTRLVARDDVPSSSFGDRAVTAEGELWHGRLHGARR
                     GEAVAEALLAVRAELEVVRAAQTALQVESDAALLEADRCMKGSAAGEQAAAAVAERVR
                     EARAGRDSLADEVTLWSRRSDEAEAEAVEADERRARDADLARELQEGLASVEARLHQI
                     GTGLDVSRAALRAAKEKREAIRDDVAVLEGKRSQASLLTVRLRERERAREEERTRARD
                     HARLAARSLTLARQRESALESYLPALRSLAQVATPLVRALEGELSSLETSLARSRATT
                     DDFGDALKDQGRRESDLQRGASDLNEELVRVQVAMAHLEEQAAERRRELDELRRKHLS
                     PRDVDAASVAGLHADEVLVALDRAERKRERIGPVNPLAEQEFREMEERAGFLFDQRRD
                     LEASLTELRAVIRDLDQHIETTFTEVFEATRKHFEEMVQILFPGGKGVLRLAEAEPGQ
                     DSPDGEDDPGLEGETASAPGPGIVLEIKPPRKTPRSMNLLSGGEKALAAIAFLFALFL
                     ARPCPFYVLDEVEAALDDLNLGRFLSLVRRYQQRTQFIVITHQRRSMEIADRLYGVAM
                     DTDGTSRVLSRRLGAEGPVAERA"
     gene            complement(11978..12772)
                     /gene="rnc"
                     /locus_tag="VFD74_09440"
     CDS             complement(11978..12772)
                     /gene="rnc"
                     /locus_tag="VFD74_09440"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZK49804.1"
                     /translation="MTGACSIAPDAGGSRSEPTVQGLPLLVRELPSHLLGLALTHSSW
                     VDARTTSNERLEFLGDSVLGLAVAAHLYDRFPGEDEGDLARWKAFAVSRESCETVSRR
                     LGFGDLVMTCAPAGADQRAEVAMAPAAMGNLLEALIGACFLAHGFPATMAAVIDAFFD
                     QVTFAVSGHMDFKTTLQEHLAAAGSAAASYAVVGEEGPPHDRLFTTVVTVAGEALGRG
                     RGRSIKKSEQEAAREALVALGVLPQDAPDNGVDRSRSHAGDEAADR"
     gene            complement(12922..13161)
                     /gene="acpP"
                     /locus_tag="VFD74_09445"
     CDS             complement(12922..13161)
                     /gene="acpP"
                     /locus_tag="VFD74_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZK49805.1"
                     /translation="MTKEEILAKIRDILVDQLGVEADAVTMAASFQEDLDADSLDLVE
                     LIMELEDQFGVKISDEEAEKITTVGEAVEFIAALQ"
     gene            complement(13291..14301)
                     /gene="plsX"
                     /locus_tag="VFD74_09450"
     CDS             complement(13291..14301)
                     /gene="plsX"
                     /locus_tag="VFD74_09450"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZK49806.1"
                     /translation="MGSSEERSVTLALDAEGGDHAPAEIVAGALAVASPRLHVLLVGR
                     PEVLGPLVPEGSADVEIVESEGTISSLEEPAKAVRSRPRSSIAVGARTVAEGRADGFV
                     GAGSTGAMLAAGLLLVKRVRGVKRPAIVTVLPGSDGPLVLLDAGANADCRPEHLLEFG
                     MLGSVFARLALDIAQPRVGLLNIGEEKTKGNELALGAYPLLAASGLNFVGNVEGRDLL
                     FGAADVVVTDGFTGNVALKLLEGTARAMLTRIGEAARRDVRSRVGGALLRPALRSVKA
                     ALDPEEYGGTYLLGLRGLVVICHGNSSRRAIANALEFGARAVRQGVVETVERELGASG
                     DA"
     gene            14471..16237
                     /locus_tag="VFD74_09455"
     CDS             14471..16237
                     /locus_tag="VFD74_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49807.1"
                     /translation="MRTLIRMMKIFLGPHKVGVALSLVLALATTGMGMFVPWLTKLAV
                     DRALVPQRGDLVTVFAAAIVGVGLLRMVFAFCRRIVSGRVALAVETEVRNRLFDHLQT
                     LGLDFHLRSQTGQLMSRAIADVRAVRMFLGYGLIFVMTNVATLVGVTVILLLLQPRLA
                     IMSLAPLPLILVVARAFSKRLHPSLWAIQQRVAELTAVAEERIMGIGVIKAFAIEHLQ
                     AEAFEAASDRIYRQNLEAAAIKGRYVPLLGMLPSISLMIILFYGGRAVIADQMTLGSL
                     VAFNGYVMLLIWPMRMLGMLVSWGERAVASGERVLEILEESPTVRDSPRASALAAVRG
                     VLEFDDVSFEYGGRRVIDGVTVRIERGETVALVGPTGCGKTTLAHLIPRFHDPARGTV
                     KLDGHDLRDITLSSLRGSIGLVDQDPFLFSLPVIENIRYGVPDASLERVEQAARAAAA
                     HDFIQALPKGYDTTIGERGLTLSGGQRQRVALARALLTDPAILILDDATSSVDAETES
                     RIVAAIGGLRGTRTTVIIAHRPSTVMLADRVILMEHGKISQIGPPCDVPWEEILVREA
                     EESLQRGIAAAPRARASEEEQA"
     gene            16234..18030
                     /locus_tag="VFD74_09460"
     CDS             16234..18030
                     /locus_tag="VFD74_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49808.1"
                     /translation="MSRVVTRQTAAEESGQGLRVWIQKRSVLRLVRFALKYRWQALCT
                     IVAMVAVTATGLAGPYLLRLAIDKGIAGKDLHYLGGIAILYLAAGILGAVLNGLQTYG
                     MNWVGERVVRDLRDSLFRHLTSLDIDYYSRQRAGWIVSRLTNDIEALESLLVEGVAQL
                     VVSTLTLVGATAILFRMDAHLAGATMSVIPVLLIATVIFRARASEAYGRVRVAVADVS
                     VALQETLSGIRVVQAFRREQRGSEAFAAVNERNRKANMDTVYISGAYFPGVEFLSAVA
                     TAIILLYGGRQVAAGQISVGILVAFIGYLSSFFDPVESLSELYNTFQSSGAALQKVVS
                     VLDTVPGDTEAIGGLSAGCLEGRLDCADVSFSYDRGGVDVLESLSLSIPQGQRVALVG
                     PTGAGKTTLAKLLLRFYQPTSGTVFVDGIDLRDYDIRDYRRHVGYVAQDPYLFSGTIM
                     DNIRLTEPEAEPARVIEAATALGVHELFSNLPEGYETQVQERGSRLSAGERQLVSFAR
                     AFFSEPRILILDEATSSVDPGTEHLIEAALSRLLAGRTSLIIAHRLSTVESSDRILVM
                     EAGHIVEDGDHASLLAAGGPYARLYRTQLRVP"
     gene            18324..>18801
                     /locus_tag="VFD74_09465"
     CDS             18324..>18801
                     /locus_tag="VFD74_09465"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HZK49809.1"
                     /translation="MTDREIDSTITAEGRPVSDVAELCTLGQERGYVTSDEIMGLVEE
                     LDMATEAIEDLYSQLFDLSIEVYEQSPVVLGALELPEPTLDLSIETVTHDPLRLYLKE
                     AGEIPLLTKADEIELAIRIEAGDKRAKDRMIQSNLRLVISIAKNYYTQDMDLLDLIQ"
BASE COUNT         3172 a         6662 c         5722 g         3245 t
ORIGIN      
        1 agccccatgg ccgccgaaag atcggccgag catggcttca ccccccctcc caggaccacg
       61 ggatctgccg caacgtcaac gcttcggcga cgtgcccgct tcccacatcg agagacgcgt
      121 caaggtctgc gagagtgcgg gcgaccgcaa gtgtgcgcac gaagccgcgg cccccgagga
      181 gacctctagc caaggcacct ctgaccaagc gccgcgcatc tcggtcgagc cggctctccg
      241 cttcgagtcg cagaggatgt tccctcgacg cgacaggcga agggtcttcc cccgcagccg
      301 atgattgcgg agggccatcg tgagccgtgg ccgttccaac actcgcgccg tgcggttccg
      361 caccgacgcg ctctcgtcgg aaggactgag cagctatgac tcgagcccgt accgtcgccg
      421 agttctccgg cggcgacggc atggagtcca agtcctcccg ggtgagcggc tcgacttcca
      481 cgaacacgtc gatgcgatcc aagagcggcc cgctgagtcg ccccgagtag cgggcgatct
      541 gctcggccgt gcagcgacac tcgcgctccg ggtggcctga gtagccgcac gggcacgggt
      601 tcatggctgc aacgagggcg aagcgggcag gaagacggat gctgcccccg gctcggctga
      661 tggtgacgtc gccctcctcg aggggttgcc gtagcgactc gaggacgtcc cgggcgaact
      721 ccggaagttc gtccaggaag agcacccccc tgtgtgcgag actcgcctcc cccggaccgg
      781 gcgaggcgcc accgccgatc accgcagccc gagacgccga gtggtgcggt gatcggaacg
      841 gtctggtctg gacgaggccg agttccggcc gatggagtcc ggcgacgctc catatgcgcg
      901 tcacttccag ggactcctcg cgcgacagcg gcgccaggat ggacgggact cttctggcca
      961 gcatggtctt gcccgcgccc ggcgtgccca ccaggagagc gtgatgtcct cctgccgcgg
     1021 ctatctcaag cgcccgccgg gcccgggcgt gacctaccac gtcgatgagg tccgggcact
     1081 gggacatcgg cggtgccgac ctgttccgac gggccaccca gcgcctccct ctctcgcaca
     1141 gcctcgccgc acatcgtgga tcgcgtagag ccaagaccgc ttcgcgaaga ctagccacgc
     1201 ctatgaaccc tatcccctcg accgcccaca cctcgggcag agccgccacc ggcaccagca
     1261 ggacatccct gccgtcgtct cgcgccgcct cggccaccgg caacatgccc tttacgggcc
     1321 ggactccgcc gtcgagcgca agttcgccca ccgccccggc gcgcttcaga gtatcgaccg
     1381 gcagatgccc cgaagcagca agcaccgcca gggcgatggg taggtcgaag cctgggctct
     1441 ccttccgcac gtcggcgggc gagagattca ccgtgatccg ctgagtcgga agtggcaggc
     1501 cgctgctgct ggcgccggag cgaacgcgct cccgcgcctc ccgcacagca gcacccggaa
     1561 gcccgacgac agtcatgccg ggaagcccgg gccgcacgtg cgcctccacg cgaacgggga
     1621 cggccgcggt gcctacgacc gcataccccc acgcctgagc catgcccacg tcggcgtctc
     1681 agcgcccggg gccgagtacg tcccgcaggt gggccaggct cctcaacgat ccatcgtctt
     1741 cgagcatcac cacgacgacg tcgaacctga cgttccgggt cacccgccct cgctcggcca
     1801 tccaccaggc ggccgccgcc cgcaatctgc gttccttccg cggaccgatc gattcgagag
     1861 cctcgccgaa gtatgagccc gagcgcgcct tgacctccac gatcaccaag gtggcgtcgt
     1921 cgcggcagac aaggtctatc tcgcccacgg gtgtgcgatg gttgcgggca accagttccc
     1981 agccgcgtga cacaaggtac tcacccgccg catcttcggc gtttcttcct cgctcgactg
     2041 ccgtcatcgc caccgccgtc tccttccctc gccgtcacgc ttccgcccct ggagcacgga
     2101 atcgacgata gcggcgttaa gggccgtacg gcatcggccc ttagtcggag atgggcgtcc
     2161 cgctgaagag ggcgagctgt ggaaacccga cgccggcgaa gctgtgccgg tggatctcgc
     2221 agggtccgag ttctctgaga gcggcacggt gggcctgggt cccgtacccc acgtgctgga
     2281 ggaatccgta gccggggtac gtctcgttga ggcgggccat gagtcgatcc cgagtcacct
     2341 ttgcgatcac cgacgccgca gccaccgacg cactcttcgc gtcaccccgg atcaactgtg
     2401 tcgcccgcag gcgctcatca tccaacgcga acccatccac gaaggcgatg tcatagtggt
     2461 tgtcaagccg gtagagagcg gtcttcaacg cctccagatt gcaccggtgc agaccgaccc
     2521 ggtcgatggt gacgggagac accgcgacga tgaccacctc cgacgcggcg acaaggatgc
     2581 gaggatagag agactccctg gcttcacggc tgaggacctt cgagtcggtc agacctctga
     2641 gcacaccggc cagaggtcta cggtagtcga ggctgaccgc cgcgaccatc aaggggcccg
     2701 ccagacaccc tctcccggcc tcgtcgaccc ctgcgacgac ccgcgctcca cgctcaagca
     2761 gagcgagcct gcggcgatcg aagccgaacg gatcacgaag cggcttgtgc gtgcgtccgc
     2821 tcagagtgcg gtcacctgca catgcgcctt ctacaaccag ccgatcttcc cgggcggcca
     2881 gtagatgacg aagcccttgc cgaggatgct ctccaacttg accggtccga acaccctcga
     2941 gtcgctgctc cggttgcggt tgtcccccat ggcgaagacg tagccttccg gcaccgtctc
     3001 cgaagggaac acgccttcga tgttccgttc gttcaaatac ggctcgtctt gggcgacgcc
     3061 gttgacgaag agcaatccgc ctttgacctc caccgtgtcg cctccgacag cgaccacacg
     3121 cttgatgtag tcctgctcgg gcgccgtggg cgggtggaag acgatgacgt cgcccggttt
     3181 cgggtcgttg aagcggtaca cgaagcgatt caccagcacg cggtcgccct tgaccaaggt
     3241 gggctccatc gactccgacg ggatgtagaa gggcttgacg acgaactgct ggatgaggag
     3301 cgccaacacg aaggccgcgc ccacgatgaa cacgacctcc agcagcgaac ctagtagtga
     3361 cgatcccgcc tggtcaccag acggaagtct gcgcagccgc ttcagcatca cgcgggcccg
     3421 gctagacccg cttctccttg actcgggcct tcttgccgat cttgtcgcgg aggtagtaga
     3481 gcttggcccg gtgcacgtcg ccgatggcca agacctcgag cttatcgatc ttgggcgagt
     3541 gcacggggaa ggtacgctcg actcccacgc cgaacgactg cttgcgcacc gtgaaggtct
     3601 cgcggacgcc ttcgccctgg cgcttgatgc agatgccctg gaacacctgg atccgctcgc
     3661 gcacaccctc gatgaccttg aagtgcacct tcaccgtgtc cccggccttg aagcggggaa
     3721 cgtcgtctcg tagttgttgc tgctccaacg cttgaataat gacactcatc tcagtttcct
     3781 tctgatgcgt tcatgacgcg ttttccggat ggccgtcgaa tgaagacagc gaaacttact
     3841 ccatagcccg gccgaattca acctctgcgg cggtgcgcgg ggtcctctgt cgttccgtcc
     3901 acgcgggcca gcgcgaaggt cgggcccgct ctcttcgcca ccgatcgatg gccgcatgat
     3961 tgccgcctac cagtatctcg ggcacctcgc tgcccatgaa ctgccgtggc ctggtgtact
     4021 gagggtactc gccggcgccg cccaatccct cactgaacga ctcttccgcg aggctctctt
     4081 cgttgccgag cacgccgggg atcttcctga ccacggcctc cacgacagcg aaggcggcca
     4141 cttcgccgcc ggcgagcacg tacggtccaa cggacacctc agcggatgcg aacaactcgc
     4201 ccacacgcgc atcgaagccc tcgtagcgcc cacacaacag gaccagatca cggcagtcgc
     4261 gggccagcgc ggccgcggtg gggtcgtcga agggctctcc cgccggcgcg agcagcacca
     4321 cgtcgcgctc ctcctgcacc cgatgcgcct ccacaccgaa gaccccggtc aaagccgcag
     4381 ccatgacatc cacgcggatg accatcccgg gacctccgcc gtatggggtg tcgtccacct
     4441 gccgatggtt gagcggagtg tagtcgcgga tgttgtggat cctgacgtcg acgcaccctc
     4501 gcgagagcgc gtcggcaatg ggatgctggt gcaggaacca cccgaaggcc tccgggacca
     4561 gcgtcaggat gtcgatgcgc ttcatgaggc gatgcctttc gtggctgctg cggcccaccg
     4621 gcccgccgct atccgcgggg gacgcgcttc gagtcggtgt caatcgtcgt ccgcgggcct
     4681 aaggcgctcc acatcgaccg tgacgtagcc cgactccact tctaccacgg ggacgaactc
     4741 atgtatgaac ggagccagca tctcgccgcc ggagctgagt tcgatggcga ggaggtcgtg
     4801 agccgggctc tccaacacct ccctgaccac gcctaccaca gtgccgtcga gcgacctcgc
     4861 ctccaagccc tcgagctcga acgagtagaa ctcgccgtct tcgagcgcag gaagttccga
     4921 cagcgggacc tgcagcaccc aaccgcggaa gccctctgct tgagtgcgat catctactcc
     4981 cgcgaacttc acgatgggcg aatccgccgt acccttgatg gactcgatct ccaaggtgac
     5041 gcgtccggac tccgcctggc ggagtccggg gcgactcggg actccgtgca gcacggcccc
     5101 tttgacaaac ctgtccgggt tgtcggtgct caacgtgacg cgcacatcgc cgcgcacccc
     5161 gtgcgcgcgc gatatgcggc cgacctctat ccactcggga agagacatgt cgtgcgtcgg
     5221 gccggcggaa gaccgcctag tcgacgatct ccacgatggc gcgcttgccg ttcttcacgg
     5281 cgctcgcctt gacaaccgtc ctcagcgcgc gagcgatgcg cccctgcttg ccgatgacct
     5341 tgcctatgtc ctccctcgcc acgttcaact ccaggaccac agtggtctcg gtctcggtag
     5401 actccaccct gacttcgtcg gggctatcga ccagcgcctt ggcgaggtac tccaacaggt
     5461 tcttcagctc gacttccatc tgtcgccccc cttgctcccg cggtctggac tcaactgtca
     5521 actccgaccc accgccgcgt gccacgcccc cgtgcaggca ctacgaaggc tacaggatgc
     5581 ctttgatctc cagcagtttg cgtaccgccc cggaaggttg tgcgcccttc gaaagccaat
     5641 ccttggcccg gtccgcatcc acctctaccg tggaaggttc ggtttgcgga ttgtaggtac
     5701 cgatgttctc gatgaaccgc ccgtcgcgcg gagcgcgaga gtcggccacc acgatgcggt
     5761 acataggatg cttcttgctg cccctgcggg tcagtctgat cgccgtcgcc ataagtcacc
     5821 tcctctgtct gatactcaag tctgtacgca tgtaggacgc cccggcgtca catgcccaat
     5881 gtctggaacg ggttcttcgg catcttcccg gagcccaact gcttcatcat cttctgcatc
     5941 tgcttgaact gattcagtag ttggttcacg gcctggatgt cattgcccga gccactcgcg
     6001 atccgacggc gtctgcctcc gttgatggtc tccggatgcc gtcgctcctc caaagtcatc
     6061 gagtgaatga tagcctgaat ccggtcgaag gcggtgtcat ccacctgcag gcccttcagt
     6121 ttgcccgcgg acaggcccgg aatcatacca agaatactct gaagcgatcc catcttccga
     6181 acctgctcca gctgttccag gaagtcctcg aacgtgaact gactctttcg caggcgcttc
     6241 tccagttcgc gcgccttctt ctcgtcgacc gcagcctcgg cccgctccac gagcgagagc
     6301 acatcgccca tgcccaggat gcgcgaagcc atgcgatcgg ggtggaagta gtcgaaactg
     6361 tcgagcttct cgccggtgct cgcgaacttg atcggccgac cggtgaccgc tctcaccgag
     6421 agagcggcgc cgccgcgggc gtccccgtcg agcttggtga ggatcactcc gtcgaactgg
     6481 accttctcct ggaactgctg tgccacgttc accgcgtcct ggcccgtcat cgcgtccaag
     6541 accaagagga tgttgtgagg cttcaccctc gaacgtatcc cgaccagttc atccatgagg
     6601 cgttcatcga tgtggagacg ccccgcggtg tcgatgatca cgacgtcgcg accgctttcg
     6661 cgggcccact tgaggccgtt cacggcaata tccaccggat cggaggtggt gccttcgtcg
     6721 tacaccggga cgcccagctg ttcacccagg atcttcagct gctccacggc ggccggacga
     6781 tagacgtctc ccgccaccag agcggggctc ttgccttgct tggagaggaa cgcggccagc
     6841 ttgccgcagg cggtcgtctt tcccgacccc tgcagcccca ccatcatcac caccgttgga
     6901 ggacgcggcg agaaggtcag cctggtggag gtggatccca tcagtgtggt gagctcgtca
     6961 ctgacgatct tgacgaactg gtgggcggga gtgaggcttt cgagcacctc agccccaaga
     7021 gcgcgttcct tcacgcggga gacgaagtcc ttgaccacct tgaagttgac gtcggcctcg
     7081 agtagcgcca acctgatctc tcgggccgcc cgctcgacat ccttctcact gagcttgccc
     7141 tgccccttca gcccgtcaag ggccccttgg agcttatccg acaacgaatc gaacatatct
     7201 tcctcggtcg tggaccacaa tgcgcggtcc cgccggacgt gcgctttcgg agggtcgagt
     7261 tcgaacgaga tgaccccgcc cggcgagccg ctggaggcgg agtttaccgc ccacggatac
     7321 gggcgtcaaa ccggtgccgg cggtgggagt cgcacaggtt gcgtgtccga tgcgtggatt
     7381 cacgctctgc cgcgcagaag ctcgtcgccg aagagcgcct cggtgaagcc ggcggcgtcg
     7441 aaaggttgga gggcgtccat cgtctcgccc accccgatga gtttgatcgg cacgtccagt
     7501 tcgtgcgaga tggccagcgc gatgcccccc ttggccgtgc cgtccatctt ggtgagaacg
     7561 acgccgctca cgtcgataac ttctttgaag aggcgagcct ggatcaggcc gttctgcccg
     7621 gtcgtggcat cgatggtgag aagcgtctcg tgcggcgcgt cgggcacctg cttcttcacc
     7681 acacgcacga ccttctcgag ctcgcgcatc aggttgacct gcgtatgcag ccgtccggcc
     7741 gtgtcgatga tcaccacatc gacgcctcgg gccttggccg cctcgatgcc gtcataggcc
     7801 acggcgcccg ggtctccgcc tttgtcgtgc cgcacgacct cgcatcccac gcgccggccc
     7861 cactccacga gttgctcggc ggccgccgcg cgaaaggtgt ctcccgccac cagcagtggt
     7921 tccttcccca gctccttgag atgccaggcg atcttgccga tggtggtggt cttgccggtg
     7981 ccgttcaccc ccgccatcag caggaccgcg ggctgagccg agacgtcgat gcgcgtgtcc
     8041 cccgagcaga agtgctccgc ggagatgcgt ctcaattccg ccaacaatcg agcgccgtcg
     8101 gggatgccac cggcctccgc cgtctcctcc agcctgcgga cgatggatat ggtggtgtcg
     8161 acccccacgt cggcatagat gagggcctct tcgatacgct cccagagatc ctcgtcgaag
     8221 cggtcggtga tgatggccga gaactgctgt tgcagcgcct gccggctctt gatgagccct
     8281 tcgcgcagct gccggaacca gccccgcttc ctctcgagct tggcatccgg ggggccgacc
     8341 gtggcccgcg cgtcaacgcc gatgaacacc tcggaccagt tgtacgacac cgaacctcct
     8401 cagcaggacg cgctcacacg tcaggggatg atagcagagc ccccgttccg ttcagcggcc
     8461 ccagccgggg gtcaggctcg ctccgcgacg ggaccttcgg ccccgagacg cctcgagagc
     8521 acccgggacg tcccgtcggt gtccatcgcc accccataga gccggtcggc gatctccata
     8581 ctccgacgct ggtgggtgat gacgatgaac tgtgtgcgtt gttggtaacg cctcaccagt
     8641 gagaggaacc ggcccaggtt cagatcatcc agtgccgctt ccacctcgtc caggacgtag
     8701 aacggacacg ggcgggccag aaagagcgcg aagaggaagg cgatcgccgc cagcgccttc
     8761 tcgccgccgc tcagcagatt catgctgcga ggcgtcttgc gaggcggctt gatctcaagc
     8821 acgatgccgg gaccgggagc cgaagccgtc tcgccttcga gaccggggtc gtcctccccg
     8881 tcaggcgaat cttggccggg ctccgcctcc gcgagccgca gcaccccctt gcctcccggg
     8941 aaaaggatct gcaccatctc ctcgaagtgc ttgcgggtag cctcgaacac ctcggtgaag
     9001 gtggtctcga tgtgctggtc aaggtcgcgg atcaccgccc gcagttctgt cagcgaggcc
     9061 tccagatccc ggcgctggtc gaagaggaag ccggcacgct cctccatctc gcggaactcc
     9121 tgctcggcca gcggattcac cggcccgata cgttctctct tccgttccgc tcgatccaac
     9181 gccacgagca cttcgtccgc gtggagacct gccacggagg ccgcgtccac gtccctcggc
     9241 gagagatgct tgcgccgtag ctcgtcgagt tcccgcctgc gctccgccgc ctgctcttcc
     9301 aggtgggcca tggccacctg cacccgcacc aactcctcgt tcaggtcgga cgcgcccctc
     9361 tgcaaatcgg actccctccg accctgatcc ttcaaggcgt cgccgaagtc gtcggtggtg
     9421 gctcgggacc gcgccagact cgtctcgaga gaggacagct cgccctcgag cgcacggacc
     9481 aggggcgtcg cgacctgggc caagctccgc aaggcgggta ggtagctctc cagcgccgac
     9541 tccctttgcc gcgccagcgt cagcgaacgc gccgcgagcc gcgcgtgatc tcgggcccgg
     9601 gtgcgctcct cctcccgcgc gcgctccctc tccctcaggc ggacggtgag cagggacgcc
     9661 tgcgaccgct tcccttcgag cacggccacg tcgtcgcgga tcgcctctcg cttctccttg
     9721 gcggcgcgca aggcggcacg tgagacatcc aggcccgtgc cgatctggtg gagtcgcgct
     9781 tcgacagagg ccaagccctc ctgcagttcg cgcgccaagt ctgcgtcgcg ggctcgtcgc
     9841 tcgtcagctt caaccgcctc cgcctcggct tcgtcggacc gcctgctcca cagcgtcacc
     9901 tcgtcggcca gagagtcccg acccgcccga gcttcgcgaa cccgctctgc gacggccgcc
     9961 gcagcctgct cgccggcggc ggaacccttc atgcaccggt cggcctcgag cagcgctgcg
    10021 tcgctctcga cctggagagc ggtctgtgcg gcgcgcacga cctccaactc ggccctcacc
    10081 gccaacagcg cctcggcgac cgcctctccc cggcgcgcgc cgtggagtcg cccatgccac
    10141 aactcgccct cagcggtcac cgctcgatcg ccgaacgacg aagacgggac gtcgtcccgt
    10201 gcgaccaacc gcgtcggcgg caggatggcc ttgagcgccc gaaccacgcc ctccggcccg
    10261 tctatgacgt cccagatgtc tacgccgccg gtccgggcat ccggcgcggc gggcgcagcc
    10321 ggggcttgcg ccttgccgcc gtcagggtcg ctgcgcggct cccctgcggg ccagagaatc
    10381 tccaccagac cttcgccgct ccgaagcaac tcggcatccg ttcccccgga gacgaccaca
    10441 gccgacgcca atgggccgag cgcggccgcg acggcgcgct cgtatcccgc ccgcacgcgc
    10501 aacgcctcca ctaccaggac ggcgcccgac tctgattcca ggagcgcacg ggccgcgggc
    10561 ggcgtgccct ctctcctcga cgccacctct tccaagacag tcacccgaga cgtggccgcc
    10621 gacaggcgct cagccagcga ccgggcgaga acacgggcct ggtcggcctc catctggagg
    10681 gcggccgcac ccctgcgggc gacggagacc tcctcctcga gccccgccag ggtcacttcg
    10741 gccgccgcca ccatcccggc ccgctcgcga gacgtgttcc tgacctcgtc aagccgacgg
    10801 gcggctacgc ggacggcctc ggccaactcc tcagcccgac gggttctgcc cgccatctcc
    10861 cgaacaagga agtccttctc ctgtagggcc cgagccctct cggcctcgag cccgaacacc
    10921 tcgtccctga ggacgtcctc gtcccgcgcc actgcctcaa gcgacgggcg catctcctcc
    10981 agcctagccg ctagcgactc ggccgccgag acgaccaggt tcaggcggcc ggccgcctga
    11041 gcaggcccgt caccgagcgc gtcgacgcgg gcgcgcgcca caccatcctc ctccgccgcg
    11101 atagcagccc gacgacgcgc ccgcgccagt tccgcttcca tacgagccac tcgctggtcg
    11161 agcgacgccc cgcgggacct cacgcggtcg acgtccgcag cagcctgatg gtagacggcg
    11221 ctcagagcct cgcgttcacg caaggcggtc gtgaaacgct cctcctcacg tgtacgctcg
    11281 tctcggaggg cggcgagcga cgcctccgtc tcggcgcgtc gagccgagac ctcgtcgaag
    11341 gtgctccgtt ccgtttcgat acggccctcg agcccgacca gcgcgagcag ggcgccttgg
    11401 gcgcgcagtc gcgccacttc ttccgatgct tccgcgtagc gttccgcagc cgagacctgc
    11461 tgcttcagcg gcctcagcgc ggcgcgcacc tcccgctcca cgtcgctgac gcgaagcaga
    11521 ttcgtcctga ccttctcgag cttggccagc gcccggtcac ggcgcttctt gtagcgcccg
    11581 aggcccacgc cgccgacgag atcctccacg tcgaccagtc tgctcgccgc cccgttgatc
    11641 cggtacttcg acgtgccgtc cctcgtcacg cgccggctga tctcgacttc agcgaaatcg
    11701 agggggaaga cccctttggc attgtcgaac accagggaga cctcggccac ggccgcggga
    11761 cgctgcccat cagacccgga gaagatgacg tcctgcatgg aacgcgctcg gatcgtggtg
    11821 ggactctgct ctcccagggc ccacagaacg gcgtcggcga cattgctctt tccgctcccg
    11881 ttcgggccga tgatcaccga cacgccctgc tcgaagacga gctcggtggg ccgtgcaaac
    11941 gtcttgaagc ccttcatgcg gatgcggctg aggaaggtca acggtccgcc gcctcgtccc
    12001 ccgcgtgcga cctcgaacga tcgaccccgt tgtcgggagc gtcctgagga aggaccccca
    12061 gggccaccag tgcctcccgg gcagcctcct gctcactctt cttgatactg cgcccacgac
    12121 ctcgccccaa ggcctcccca gcaacagtca caacggtcgt gaacaaccgg tcgtgcggcg
    12181 ggccctcttc acccaccacc gcgtacgagg ctgcggcgct ccctgcagcg gcaagatgct
    12241 cctgcaaagt ggtcttgaag tccatgtgac cgctgacggc gaaggtcacc tggtcgaaga
    12301 acgcgtcgat gaccgccgcc atggtcgccg gaaacccgtg cgccaagaaa caggccccga
    12361 tgagcgcctc cagcaggttg cccatcgctg cgggagccat cgcgacctcc gcccgctggt
    12421 cggcccccgc cggggcgcag gtcatcacaa ggtcgccgaa acccaggcgg cgggacaccg
    12481 tctcacagct ctcacgactg accgcgaagg ccttccacct agcgaggtcg ccttcgtcct
    12541 cgccggggaa gcgatcgtag agatgggcgg ccacagccag gccgaggaca ctgtccccca
    12601 ggaactcgag gcgctcgttg gacgtagtcc gggcgtcgac ccacgacgaa tgggtcaaag
    12661 ccaggccgag gagatgggac ggcagctcac gaacaagtag cggcagcccc tgtactgtgg
    12721 gttctgagcg actgcccccg gcgtcggggg cgatcgagca ggcgccggtc acgccgcacg
    12781 cacttcccgg tcagccgaca ccgggagcga gtcctgcgac tctccggaac agatcgaggg
    12841 gtctgccgct tgcagcggca gacccctcag agatccgcgc gcccgacgag accggctcgg
    12901 ccgacgcgaa gggatcatca actactggag ggcggcgatg aactcgaccg cctcgccgac
    12961 agtcgtgatc ttctcggctt cctcgtcgga gatcttgacg ccgaactggt cttccagctc
    13021 catgatcagc tcgaccagat cgaggctgtc cgcatctagg tcttcctgga acgaagccgc
    13081 catggtgacc gcgtcggctt caacgccgag ttggtccacc aagatgtcgc ggatcttcgc
    13141 gaggatctct tccttggtca tcaatactcc tcatggatcg ggatcttctt tgaacgtaga
    13201 cgggcaccgc gtcattctga tcttcctcga acgtcaacgg gcaccgtgtc attctagcaa
    13261 cgtgcgcgtc ctaggcaaag gaccgcgccc tcaagcgtcg ccgcttgcgc cgagctcgcg
    13321 ctccacggtc tccacgactc cttggcgcac ggcgcgcgcg ccgaactcga gcgcattcgc
    13381 gatggctctc ctcgacgagt tcccgtggca gatcaccacc agccccctca agcctagtag
    13441 gtaggtgccc ccatactcct cggggtcgag ggccgccttc acactccgga gcgcaggtct
    13501 caggagcgct ccgcccaccc gggagcgcac gtccctgcgg gccgcctccc cgatgcgggt
    13561 gagcatcgca cgcgccgtcc cctcgagcag cttcagggcg acgttccccg tgaacccgtc
    13621 ggtgacgacg acgtcggcgg ccccgaagag caggtcgcgg ccttcgacgt tgcccacgaa
    13681 gttgagacca gaagccgcca acagaggata ggcccccagc gcgagctcgt tccctttggt
    13741 cttctcttcg ccgatgttca gaaggcccac ccgcggttga gcgatgtcga gggcgagacg
    13801 cgcgaacacc gagccgagca tcccgaactc gagcagatgt tccggacgac aatcggcgtt
    13861 cgcacccgcg tcgagcagta cgagaggacc gtctgagccc ggcagcacgg tcacgatggc
    13921 cgggcgcttg actccacgca cccgtttcac gagcagcaga ccggcagcga gcatcgcgcc
    13981 cgtgcttccc gccccgacga agccgtcggc cctcccttcg gccaccgtgc gggctcccac
    14041 cgcgatcgat gatcgcggtc gggagcggac ggccttcgcc ggctcttcaa gcgacgagat
    14101 agtgccctcg ctctccacga tctccacgtc agccgacccc tcggggacca gggggccgag
    14161 cacttccggg cgaccgacca agagcacatg cagcctcggg gacgccacgg cgagtgcacc
    14221 cgccactatc tccgcaggag cgtggtcgcc cccttcagcg tcaagagcca acgtcaccga
    14281 gcgctcttcg ctcgaaccca tcgatgtctc ctcttccgat acgatcaccg catcaccgcc
    14341 ggcgggcccc gggtccgacc ccgcgcggct ccgcccccgg ttttcgactc tcattaccat
    14401 accagccttc gatccttctc ggcccggtga gagaagagcc cagtcacccc tgcgctacaa
    14461 tctagcccgc atgcgtactc tcatccggat gatgaagata ttcctcgggc ctcacaaggt
    14521 cggcgtcgcc ctgtccctgg tgctcgccct ggccaccacc ggcatgggga tgttcgtacc
    14581 ctggctcacg aagctcgcgg tggatagagc cctcgttcct caacgtggcg atcttgtcac
    14641 ggtgttcgct gccgccatcg tgggagtagg cctgttgcgg atggtcttcg ccttctgccg
    14701 acggatcgtg tcgggccgag tggcgcttgc cgtggagacc gaggtccgga accgcctctt
    14761 cgaccacctc cagacgctcg ggctcgactt ccacctgcgt tcgcagacgg gccagctcat
    14821 gtcccgcgcc atcgccgacg tacgcgccgt tcgtatgttc ttgggttacg gactcatatt
    14881 cgtgatgacc aacgtggcca ccctcgtcgg ggtcaccgtc atcttgctgc ttcttcagcc
    14941 caggctcgcg atcatgagcc tcgcgccttt accactcata ctcgtggtgg cccgcgcgtt
    15001 cagcaagcgc ctgcacccct cgctctgggc catccaacaa cgagtggcag aactcaccgc
    15061 ggtcgccgaa gagcgcatca tgggcatcgg ggtcatcaag gccttcgcga tcgagcacct
    15121 gcaggcggag gccttcgagg ccgcctccga ccgcatctac cgacagaatc tggaagccgc
    15181 cgccatcaaa ggccgatacg tgccgctgtt gggaatgctg ccgagcatca gccttatgat
    15241 catcctgttc tacggcggcc gcgcggtgat agccgaccag atgacgcttg gcagcctcgt
    15301 ggccttcaac ggatacgtga tgctcctgat ctggcccatg cggatgctgg ggatgttggt
    15361 gagttggggc gagcgggcgg tggcttcggg agagcgggtc ctcgagatcc tcgaggagag
    15421 ccccacggtg cgcgactcgc ccagagcgag tgcgctggca gccgtccgcg gtgtgctgga
    15481 gttcgatgac gtctccttcg agtacggtgg caggcgggtg atcgatgggg tcaccgtgcg
    15541 catcgaacgc ggcgagaccg tggccctggt gggccccacc ggatgcggca agacgacact
    15601 ggcccacttg atacccaggt tccacgaccc cgcacgcggc actgtgaagc tcgacggtca
    15661 tgatctgcgc gacatcaccc tctcctcctt gcgcgggtcc ataggcctgg tggaccagga
    15721 ccccttcctc ttcagcctgc ctgtgatcga gaacatacgc tacggcgttc ccgatgcatc
    15781 tctggagagg gtggagcagg cggcccgcgc ggcggcggcg cacgacttca tccaggcgct
    15841 tccgaaaggg tacgacacga ctatcggaga gcgcgggctt acgctgagtg gaggacagcg
    15901 tcagcgagtc gcgctggcgc gggccctgct caccgatccg gcgatcttga tcctcgacga
    15961 cgccacttcg agcgtggacg cggagacgga gtcccgcata gtcgcagcga tcggggggct
    16021 tcgcggcacc cgcaccacag tgatcatcgc gcatcggccg tcaacggtca tgttggccga
    16081 cagagtcatc ctcatggagc acgggaagat ctcccagatc ggcccgccct gcgacgtgcc
    16141 ctgggaagag atcctcgtgc gagaggccga agagtcgttg cagcgtggta tcgccgcggc
    16201 ccccagagcc cgcgcttccg aggaggagca ggcatgagtc gcgtcgtgac ccggcaaacg
    16261 gcggctgagg agagcgggca gggtctccgc gtatggatac agaagcggtc ggtcctccgc
    16321 ctcgtgcggt tcgctctgaa ataccgttgg caggcgctat gcacgatagt cgcgatggtc
    16381 gcagtgacag cgaccggact ggccgggccg tacctcctgc ggctcgccat cgacaagggt
    16441 atcgccggta aggacctgca ctatctcggc ggcatcgcga tcctgtacct cgccgcgggg
    16501 atcttgggcg ccgtgctcaa cggcctgcag acatacggca tgaactgggt gggagagcgc
    16561 gtggtgcgcg acctgcgcga cagcctgttc cggcacctca cctccctcga tatcgactac
    16621 tacagccggc agcgagcggg gtggatcgtg tctcgcctga ccaacgacat cgaggccctt
    16681 gagagtctgc tggtcgaggg ggtggcacag ctcgtcgtga gcaccctcac cttggtaggc
    16741 gccacggcca tactcttccg catggacgca caccttgctg gggccacgat gtcggtgatc
    16801 cctgtgctgc tcattgccac ggtcatcttc cgcgcacgcg cttcggaggc ctacggtcga
    16861 gtgagggtgg ctgtcgcgga cgtcagcgtc gcgctccagg agacgttgag cgggatccgt
    16921 gtagtgcagg cctttcggcg cgaacaacgt ggctcggagg ctttcgccgc ggtcaacgag
    16981 cgcaaccgca aggccaacat ggacaccgta tacatctccg gtgcgtactt cccgggcgtc
    17041 gaattcctga gtgcggtcgc caccgccatc atcttgctct acggcggccg ccaggtggcg
    17101 gccggtcaga tctcagttgg aatccttgtg gccttcatcg gatacctgtc cagcttcttc
    17161 gacccggtcg agtccctgtc cgagctctac aacaccttcc aatcctcggg cgccgccctg
    17221 cagaaggtgg tatcggtgct cgataccgtc ccgggcgaca ccgaagccat cgggggtctc
    17281 tccgcgggat gcctcgaagg ccgtctcgac tgtgccgacg tctcgttctc gtatgatcga
    17341 ggaggggtcg acgtactcga gtcgctcagt ctgtccatcc cccaggggca acgggtggcc
    17401 ctggtgggac ccaccggagc cggtaagacc acgctggcca agctcctcct gcgcttctac
    17461 caaccgactt cgggaaccgt attcgtcgac gggatcgacc tgcgtgacta cgatatccgc
    17521 gactaccgcc gccacgtcgg atacgtcgcc caggatcctt acctcttctc gggcaccatc
    17581 atggacaaca tacgactcac cgaacccgag gccgaaccgg ctcgtgtcat cgaggccgca
    17641 accgctctgg gtgtgcacga actcttctct aaccttccgg aagggtatga gacccaagtc
    17701 caggaacgcg gctcgaggct gtctgcgggc gagcgacaat tggtgtcgtt cgcgcgcgcg
    17761 ttcttctccg aacctcggat cctcatactc gatgaggcta cctccagcgt ggaccccggc
    17821 accgaacatc tgatcgaagc ggcgctctcc cgactactcg ccgggcgcac ctcgctcata
    17881 atcgcccacc gcctctcgac cgtcgagagt tccgatcgca tcctggtcat ggaggcgggg
    17941 cacatcgtcg aagacggtga ccacgcgtcg ctactcgccg cgggcggacc gtacgcccgg
    18001 ctctatcgca cccaactccg cgttccctag cgtcgccgcg ggtcctggac cccgcggcca
    18061 cactcacgca acctgacctt gcgcaacgaa cgtttgttcg gtatagtggg aaaggtgtct
    18121 cgtgctcgtt atcggaagcg accgtgtcta tacagcgcga gccccaagag caccgcaact
    18181 ttccgaagaa ggtatggact tggacacccg tgaggtatac aatgcacggg tttgcctgac
    18241 gaggcttgca caaaacatcg tgcgaagtgc gtcgctccag ctacgcacac ggcacgttat
    18301 caaggaatgg ttggtcgaag catatgacgg acagagagat tgattcgacg ataacggcgg
    18361 agggccgccc cgtttccgat gttgcagagc tctgcacact cgggcaggaa cgcggctatg
    18421 tcacgagtga cgagatcatg ggtctggtgg aagagcttga catggcgaca gaggctatcg
    18481 aggacctcta ctcgcaactc ttcgacctct cgatcgaggt ctacgagcag agtcccgtgg
    18541 tgctaggagc cctggagctt cccgagccca cgttggacct ctctatcgag acggtcacgc
    18601 acgacccctt gcggctctat ctcaaagaag caggcgagat cccccttctc accaaggccg
    18661 acgagatcga actcgccatt cgcatcgagg ccggggacaa gcgggccaag gatcgcatga
    18721 tccagtctaa cctgcgccta gtcatctcga tcgccaagaa ctactacacc caggatatgg
    18781 atctgctcga tcttatccag g
//
LOCUS       DASRMJ010000338         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_431810, whole genome shotgun sequence.
ACCESSION   DASRMJ010000338 DASRMJ010000000
VERSION     DASRMJ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431810"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFD74_09470"
     CDS             complement(<1..254)
                     /locus_tag="VFD74_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZK49810.1"
                     /translation="MAKVLMIHPDKCTGCRNCELACSFEHEAQFRPGASRVQAYTWER
                     EGMSVPMMCQQCDDAACVNVCPTGAMHRSTTMANLVDWDE"
     gene            733..1440
                     /locus_tag="VFD74_09475"
     CDS             733..1440
                     /locus_tag="VFD74_09475"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZK49811.1"
                     /translation="MPDAATLVEKVQASTYARLFEHVWGPGAFSAVDMAYDNIGRSIA
                     AYERSSEVSAFTSKYDAWVAHKAKLTGQEMKGFRLFMHKAKCGTCHVPPRFTDFTYDN
                     LGIPKNPLNPVYDWAGSSWVDNGLGGYLVTVPQFETQAASEMGKFKVPTLRNVDKRPS
                     VTFVKAYGHNGYFKSLESIVHFYNTRDVANWPAPEVAVNVNATAMGNLRLTPNQEKAI
                     VAFLKTLSDGYGSASCH"
     gene            complement(1968..>2177)
                     /locus_tag="VFD74_09480"
     CDS             complement(1968..>2177)
                     /locus_tag="VFD74_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZK49812.1"
                     /translation="AHNLGIWVNLMWLQGAPLRMKTLRQRYLTLPGRLTRGARRLHLA
                     LPVAWPWKEQFLAALMRLRALPLLA"
BASE COUNT          357 a          747 c          735 g          338 t
ORIGIN      
        1 ctctcgtccc agtccacgag gttcgccatc gtggtgctgc ggtgcatggc gcccgtgggg
       61 cagacgttga cgcaggccgc gtcgtcgcac tgctggcaca tcatcggcac cgacatgccc
      121 tcgcgctccc aggtgtaggc ctgcacgcgc gaggctcccg ggcggaactg ggcctcatgc
      181 tcgaacgagc aggccagttc gcagttgcgg cacccggtgc acttgtccgg gtggatcatc
      241 aggacctttg ccatggcgtc tcctttggcg atgggtgcac gggacgagac ggagcggccg
      301 agtggcagct accgtcgatg gctgtcggtg gggggagcgc ggaggcgcgg cactcgactg
      361 cggccgtccg cgcaacggca gacggacctc gagtgaggat gcgctttggc tgagccgggt
      421 acccgcgccc attcatccgg gtgtccacgg cgtcctctct tcggcggcac gtcaggcgtc
      481 acgtgccacg agacgtctcc tctaccttac ccgaggcaac ggcttcgcaa gcggcggcaa
      541 cgcgggatgc catgagcaaa ttcgggagag cctggggcgg ctcgggcacg ggctgttctt
      601 cacggcgccg ggcgcggtct acgagggctc ggacgccgga atgttcggca accgcaagcg
      661 accggcggga cgctgggcga cccgctcgcc gagcaggcga aagggccgtt cctgaacccg
      721 cgcgagcagg ccatcccaga cgcggctacc ctggtcgaga aggtccaggc ctccacctac
      781 gcacggctgt tcgagcacgt gtggggtccc ggggcctttt ccgccgtcga catggcctac
      841 gacaacatcg gccggtccat cgccgcctac gagcgctcct ccgaggtgag cgcgttcacc
      901 tccaagtacg acgcgtgggt ggcccacaag gcgaagctga ccggccaaga gatgaagggc
      961 ttccggctct tcatgcacaa ggcgaagtgc ggcacctgcc acgtaccgcc gcgcttcacc
     1021 gacttcacgt acgacaacct gggcatcccc aagaaccccc tgaaccccgt ctacgactgg
     1081 gcgggctcct cctgggtcga caacggcctc ggtggctacc ttgtgaccgt gccccaattc
     1141 gagacacagg ccgccagcga gatgggcaag ttcaaggtcc cgaccctgcg caacgtggac
     1201 aagcgcccgt cggtgacgtt cgtaaaggcg tacggccaca acggctactt caagagcctg
     1261 gagtccatcg tccacttcta caacacgcgt gacgtggcca actggccggc ccccgaggtg
     1321 gcggtcaacg tcaacgcgac cgcgatgggg aacctccgcc tgacccccaa ccaggagaag
     1381 gccatcgtcg ctttcctgaa gacgctgagc gacggctatg gctccgcttc ctgccactag
     1441 ttgaagcgaa acgggcgggg cgcccgcggc gccccgccct cgccttgttg ggcggggtcc
     1501 ctgcgtcggc ggatccgggc tgagccgcct ggtgacgacc cgcctcatgg tcgccttcgt
     1561 ccgctcgtcg acgggcggct ccgggagcaa ccagtgcatc gatgggcgtg cggaatgtgg
     1621 tctcgttctc gatcctggtc ctgctcgtgg cgcgcggcgt gagtatcgcc cattgagagc
     1681 cggctcacac gggctcatcc tgaccgccgc acgtcggggg cgtcccacct tgtgcccgac
     1741 acctaagccc aaatccaccg atcaacccct ccaaaccgac ctgacctgcc ccggaaggct
     1801 tacgccggcc ttctcggcaa ctgaagacca cgtcaggtgg ccggaagcgc tcagatgagg
     1861 ctcgtggcga gccgccgaga gctgtccgcg gctgccgcag gccaaccgcg ggcaagccga
     1921 ggccggccgg gtccagctct ggctgatggt gagggcgccc ttctcgctca ggcaagaagg
     1981 ggcagtgcgc gcaggcgcat aagcgccgct aggaactgct ccttccaggg ccaggcgacg
     2041 ggcagcgcca ggtgcaggcg ccgcgcccca cgggtgagcc tgccggggag ggtgaggtag
     2101 cgctgccgca gggtcttcat gcgcaggggc gcaccctgca gccacatgag gttcacccag
     2161 atgccgaggt tgtgggc
//
LOCUS       DASRMJ010000339         3853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_357197, whole genome shotgun sequence.
ACCESSION   DASRMJ010000339 DASRMJ010000000
VERSION     DASRMJ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357197"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFD74_09485"
     CDS             <1..529
                     /locus_tag="VFD74_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK49813.1"
                     /translation="EPDRGRIIVGGRDLEALRRWKVPYLRRNIGCVFQDFKLLQNKTV
                     FDNVYYALQVTGNASDSGRRKTGEIINLVGLGHKVKNLPNELSGGEQQRVSIARAFVN
                     HPPLLIADEPTGNLDPDTSAGIMQLLYRINKTGTTVVMATHDREMVDKMRRRVVALEQ
                     GQVVRDQRRGSYGED"
     gene            516..1394
                     /gene="ftsX"
                     /locus_tag="VFD74_09490"
     CDS             516..1394
                     /gene="ftsX"
                     /locus_tag="VFD74_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZK49814.1"
                     /translation="MGKIKFFLSEAFRSLWRNYFMTIAAVVTVFLSMFLLGVVMVFVY
                     NINAVLKDVEQKVEITIYLKESATGDEIEAMRQEIVDLPEVKEVQFIDKDEALKRLKE
                     DLKDHEELLKDLPSNPLPASFEVALKDPKLAGTVAVKFDGRPIVEEVRYGKEIAERIF
                     KVTSVVRNISGVFVLLLGAVSILLISNTIRLSIYARRREVEIMKLVGATNWFIRWPFI
                     IEGLTVGLLGAASALALVILGTGFVMDRIRENLVFLTVPFDAVSTVQITLVILGVGAV
                     IGAVGSGLGLRRFLNV"
     gene            1399..2730
                     /locus_tag="VFD74_09495"
     CDS             1399..2730
                     /locus_tag="VFD74_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZK49815.1"
                     /translation="MPIVRTGTPVSIRRRPTAVGRLLKAAPAALLLAVVLCMGAAVAP
                     GASPSSHLGLGRLIAPGTAAAATKSQINQNLTQNQAELEKARAEILKAEAARKGALED
                     VAVLDQRIDNLESQLAKVTSERDQVAADLEATREELAGLGARLVETRARLAKAEDDLR
                     RAQSALDQRAVNIYKSGGIGYLEVLLDTARLSELITRLDFLAFIVQQDERILRQVRDL
                     RAQVDAERLDLEAQQKRISEVEGRQAGQAARLEVLVADQASKLAQVESARRDKQRVVD
                     KAESDKASWEKQEAALQAESDRLRDELRVLTAKVNQTIKGTGQFIWPVQGRVSSPFGY
                     RIHPIFKVRRLHTGIDLSASMGTPIRAADSGVVIEAGWRGGYGKAVVISHGGGLTTLY
                     AHQSSILVSVGEAVEKGDVIGKVGSTGYSTGPHLHFEVRVNGNPVDPIGYL"
     gene            2739..>3853
                     /locus_tag="VFD74_09500"
     CDS             2739..>3853
                     /locus_tag="VFD74_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZK49816.1"
                     /translation="MQRIVKSSVAVVAIWALLAVLVAGGVILGAHPASRNLLRSVLPA
                     YLADTLIGESAGFPLQDEVLQKLESSFYQPVEAGQLEDDSIRGMLKGLNDDYTNYLDP
                     EQYSAFKEHAGGSYSGVGMTVEMKGPYVTVVSTFRGSPAAEGGIEPGDVILAVDDEEI
                     DGLSLDDVVMRIKGREGSEVRLKVYHVPPGATSASAKDGIDPHLPEGGTAEDILLVRK
                     SIEIPVIEVETLRAGGHKVAHITFVTFSEDSAQHLRDAVTKAVEEGKVSAVILDLRRN
                     GGGLLNEAVDVASIFIPDGVIVTTQGLHSPEEVFTAQGTAVDPSIPLYVLVDEFSASA
                     SEIVAGALKDTGRGTLVGSTTFGKGLVQTILSLSNGGA"
BASE COUNT          712 a         1219 c         1287 g          635 t
ORIGIN      
        1 cgaacccgac cgcggacgca tcatcgtggg ggggcgtgac cttgaggccc tgcgcaggtg
       61 gaaggtgcca tatcttcgcc gcaacatcgg ctgcgtgttc caggacttca agctgctgca
      121 gaacaagacg gtgttcgaca acgtctacta cgctctgcag gtcaccggga acgcttccga
      181 ctccggtcgt cgcaagacgg gggagatcat caacctcgtc ggcctcggcc acaaggtcaa
      241 gaacctcccc aacgagctct cgggcggcga acaacagcgc gtctccatag cgcgggcgtt
      301 cgtgaaccat cccccgctcc tcatcgccga cgaacccacg ggcaacctcg accccgatac
      361 ttcggcgggc atcatgcagc tgctctaccg catcaacaag accggcacca ccgtggtcat
      421 ggccacgcac gaccgggaga tggtggacaa gatgcggcgc cgggtcgtcg ctctcgagca
      481 gggtcaggta gtccgcgacc agcgaagggg tagctatggg gaagattaag ttcttcctgt
      541 ccgaggcgtt ccgctcactg tggcgcaact acttcatgac catcgcggcc gtcgtcacgg
      601 tgttcctcag catgttcctg ctgggggtgg tcatggtgtt cgtctacaac atcaacgcgg
      661 ttctcaagga cgtggagcag aaggtggaga tcaccatcta cctgaaggaa tccgcaacgg
      721 gcgacgagat cgaggccatg cgccaggaga tcgtcgacct gccggaagtg aaggaagtcc
      781 agttcatcga caaggatgaa gctctcaaac gtctgaagga agacctcaag gaccacgagg
      841 agctgctgaa ggacctgccg agcaatccgc tgcctgcgtc tttcgaagtc gcgctcaagg
      901 acccgaagct ggcggggacc gtggcggtca agttcgacgg gcgccccatc gtggaagagg
      961 tgcgctacgg caaggagatc gccgagcgta tcttcaaggt cacctcggtg gtgcgcaaca
     1021 tctccggcgt gttcgtcctg ctactcggag cggtctctat ccttctcatc tcgaacacga
     1081 tccgcctctc gatctacgcg cggcggcgcg aagtggagat catgaagctc gtgggagcca
     1141 ccaactggtt catccgctgg cccttcatca tcgaggggct gaccgtaggc ctactggggg
     1201 cggcgtccgc tctggcgctc gtcatccttg gcaccggttt cgtcatggat cgtatccgcg
     1261 agaacctggt gttcttgacc gttccgttcg acgcggtctc gaccgttcag atcacccttg
     1321 tcatccttgg ggtcggcgca gtcatcggcg ccgtaggctc ggggttggga ctcaggaggt
     1381 tcctgaacgt ctgatcgcgt gcccatcgtg aggacgggga cgcccgtctc gatccgccgt
     1441 cggcccaccg cggtcggtcg cctgctgaag gccgcgccgg cggcgctgct gctggcggtg
     1501 gtgctgtgca tgggcgccgc cgtcgcaccc ggtgcctcac cgtcgtcgca cctcgggctc
     1561 ggccgcctga tcgctccggg aacggccgca gccgcgacca agtcgcagat caaccagaat
     1621 ctcacgcaga accaagcgga gctggagaag gcccgggccg agatactgaa ggccgaagcg
     1681 gcgcgcaagg gggcgctcga ggacgtcgcc gtcctcgacc agcgcatcga caacctggaa
     1741 tcccagctgg ccaaggtcac ctcggagaga gaccaggtgg ccgccgatct cgaggctacc
     1801 cgtgaggaac tcgccggcct cggtgcccgg ttggtggaga cacgggcgcg cttggccaag
     1861 gccgaggacg atctgcggcg ggcccagtcc gccctcgacc aacgggcggt caacatctac
     1921 aagtcgggcg gcatcggcta ccttgaagtg ctgttggaca ccgcccgctt gtcggagctg
     1981 ataacccgtc tcgacttcct cgcgttcatc gtgcagcagg acgaacgcat cctgcgccag
     2041 gtcagagacc tgcgcgccca agtggacgcg gagcgactcg acctcgaagc gcaacagaag
     2101 cgaatatctg aggtcgaagg ccgacaggcg ggacaggccg ctcggttgga agtgctcgtg
     2161 gccgaccagg cttccaagct cgcccaggtg gagagcgccc gccgggacaa gcagcgcgtg
     2221 gtggacaagg cggagagcga caaggcgtcc tgggagaagc aggaggccgc gctacaggcc
     2281 gaatcggacc gcctgcgaga cgaactgcgg gtgctcaccg ccaaagtcaa ccagacgatc
     2341 aaagggacgg gtcagttcat ctggcccgtg cagggccggg tcagctcacc gttcggctac
     2401 cgtatccacc cgatcttcaa ggtgcgcagg ttgcacaccg gcatcgacct ctcggcgtcg
     2461 atgggcaccc ccatccgcgc ggcggacagc ggcgtggtga tcgaggcggg atggcggggg
     2521 ggctacggca aagccgtggt gatctcgcac ggcggcggtc tcaccacact ctatgcccac
     2581 cagtcgagca tcctggtctc ggtgggtgag gcggtggaga agggcgacgt catcgggaag
     2641 gtggggagca ccggctactc gaccgggccg caccttcact tcgaggtgcg cgtgaacggg
     2701 aatcccgtgg atcccatcgg ctacctctag ggcccggttt gcagcgcatc gtcaagagtt
     2761 ccgtggcggt cgtggcgata tgggcgctgc tcgccgtcct cgtcgccgga ggagtgatcc
     2821 tgggggccca ccccgcttcg cgtaatctac tgcggtccgt cttgcccgcc tacctcgccg
     2881 acacgctgat aggcgagagc gccggcttcc cgctgcagga cgaagtgctc cagaagctgg
     2941 aatcgtcctt ctaccagccg gtcgaagcgg gtcagctcga ggacgactcg atccgaggca
     3001 tgctcaaggg cctgaacgac gactacacca actacctgga ccccgagcag tactccgcgt
     3061 tcaaggaaca tgccggagga tcgtactccg gggtgggcat gacggtcgag atgaaggggc
     3121 cctacgtgac ggtggtctcc accttccgcg ggtcgccggc ggctgaaggc ggcatcgaac
     3181 cgggcgacgt catcctcgcc gtcgacgacg aggagatcga cgggctctcg ctcgacgatg
     3241 tggtcatgcg catcaaaggg agagaaggca gcgaggtcag gctcaaggtg taccacgtgc
     3301 cgcccggcgc cacgtcggcc tccgcgaagg acgggatcga tccgcacctg ccagaaggcg
     3361 gaacggccga ggacatcctc ctggtgcgca agagcatcga gatcccggtg atcgaggtcg
     3421 agacgctgcg cgccgggggg cacaaggtag cgcacatcac gttcgtcacc ttctctgagg
     3481 actcggctca gcacctgcgc gacgcggtga cgaaggcggt ggaggagggc aaggtctccg
     3541 ccgtcatcct cgatctgcgg cgcaacggcg gaggcctgct caacgaagcg gtcgatgtcg
     3601 ccagcatctt catccccgac ggggtgatcg tgaccaccca gggcctgcac tcgcccgaag
     3661 aggtcttcac ggcgcagggc actgccgtcg acccctcgat cccgctgtac gtgttggtgg
     3721 acgagttctc tgccagtgcg tcggagatcg tggccggagc gttgaaggac acgggtaggg
     3781 gcaccctcgt cggcagcacg acgttcggca aggggctggt gcagacgatc ctgtcgctga
     3841 gcaacggcgg tgc
//
LOCUS       DASRMJ010000340         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_281577, whole genome shotgun sequence.
ACCESSION   DASRMJ010000340 DASRMJ010000000
VERSION     DASRMJ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281577"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1161)
                     /locus_tag="VFD74_09505"
     CDS             complement(13..1161)
                     /locus_tag="VFD74_09505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK49817.1"
                     /translation="MDDHLTSASPIIDAHVHIFSPDVIRDRRSYVEKDFWFRTLYADP
                     TSRMVPYEDVLQEMDATGVDRSVLTGFAFRDQGLCRDTNDYVIEAVRAHPDRFIGLAC
                     VSPEMPGALSELERCLDAGLRGCGELFPDGQDFDLMDSSGLDAVAVALTERGLSLNIH
                     ANEPVGHVYPGKGANTPGPCYEFARRHPDLAIVFAHMGGGLFLYEMMAEARRVLSNVY
                     YDTAAVPYLYRRDIYSVAALITGPGKILFGSDYPLLSPGRYLRDTEGLDPTIRAALFG
                     GNAVRVLGLDPSGRDATAGPAAAGLAAGREPGAVAVARGAHASTLPEDAESGGRPRGG
                     PAAGPAARPAAGPAAASRPDGSRQNTRTALPPKRAARMVGSRPSVSRR"
     gene            complement(1170..2111)
                     /locus_tag="VFD74_09510"
     CDS             complement(1170..2111)
                     /locus_tag="VFD74_09510"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZK49818.1"
                     /translation="MDLKSKRIGVLMGGLSGEREVSLRSGENCLRALQSLGYDVVRID
                     ALRDVAQRLTAERVEVAFLAVHGRYAEDGTLQGLLEILGIPYTGSGVLASALGMNKVA
                     TKKIVRHGGVPTPDFYELGDTEDAAEAARRAEALLGLPVMVKPVEEGSSLGVTKCADV
                     AQMAEAFREGRPRFGRMFAEGFVSGVEITVGVVQRGDTLDVLPILELVPKNEFYDYEA
                     KYTEGMTEFILPARLSTAVEAEVARLSVASFRSVGCRGYARVDFMVASDDTPYFTELN
                     TLPGMTDLSDLPAQAKAAGITYEQLVETILHSAVLSV"
BASE COUNT          371 a          781 c          776 g          336 t
ORIGIN      
        1 gtctccgggc cgttatctcc gcgacactga aggcctcgac cccaccatcc gagcggccct
       61 cttcgggggc aacgcggtgc gagtgttctg cctggacccg tcgggccgcg acgcggccgc
      121 gggacctgct gccggacgcg ccgccggacc tgccgccgga ccccctctcg ggcggccgcc
      181 ggactcggcg tcctcgggga gcgtcgaagc gtgagcgccc cttgcgacgg cgacggcgcc
      241 gggctcgcgt cctgcggcga gtccggcagc agcaggtccc gcggtcgcgt cgcggcccga
      301 ggggtccagg ccgagcactc gcaccgcgtt gcccccgaag agggccgctc ggatggtggg
      361 gtcgaggcct tcagtgtcgc ggagatagcg gcccggagac agcaacgggt agtcgctgcc
      421 gaagaggatc ttccccggcc cggtgatcag cgccgccacc gagtagatat cgcgtcggta
      481 cagatagggg acggccgcgg tgtcgtagta gacgttggag agcaccctgc gggcctcggc
      541 catcatctcg tagaggaaga gcccgccgcc catgtgggcg aacacgatgg cgaggtcggg
      601 gtgtcttcga gcgaactcat agcagggccc gggcgtgttc gcacccttcc ccggatacac
      661 gtgacccacc ggctcgttgg cgtggatgtt gagcgagagc cccctctcgg tgagcgcgac
      721 ggccaccgcg tccaacccgg acgagtccat caggtcgaag tcttggccgt cggggaagag
      781 ctccccgcac ccgcgcagac cggcatcgag gcagcgctcg agctcggaca gggcgccggg
      841 catctcggga gacacgcagg cgaggccgat gaagcgatcg ggatgcgccc tcaccgcctc
      901 gatgacgtag tcgttggtgt cgcgacacaa gccttggtcg cgaaaggcga agccggtgag
      961 caccgagcga tcgacgccgg tggcgtccat ctcttggagc acgtcctcgt agggcaccat
     1021 ccgtgacgtc gggtccgcgt agagggttcg gaaccagaaa tccttctcca catacgatct
     1081 tcggtcccgg atcacgtcgg gcgagaagat gtgcacgtgc gcatcgatga taggggaagc
     1141 ggaggtcaag tgatcgtcca cgaccgagcc tacaccgaca gaacagcgct gtgcaggatc
     1201 gtctccacca gctgctcgta ggtgatgccg gcggccttgg cctgagcggg gaggtcggag
     1261 aggtcggtca tgccgggcag cgtgttcaac tcggtgaagt agggcgtgtc gtcgctcgcc
     1321 accatgaagt cgacgcgggc gtagcccctg cagcccaccg agcggaacga cgccacgctg
     1381 aggcgcgcca cttccgcctc caccgccgtc gacaaccgcg ccggcaggat gaactcggtc
     1441 atgccttcgg tgtacttggc ctcgtagtca tagaactcgt tcttgggcac caactcgagg
     1501 atcggcagga cgtcgagagt atccccccgc tgcaccaccc ccacggtgat ctccacgccg
     1561 gacacgaagc cttccgcgaa catgcgcccg aacctcggcc gaccttcacg gaaagcctcc
     1621 gccatctgcg cgacgtcggc gcacttggtc accccgagac tggagccctc ctccaccggc
     1681 ttgaccatca ccggcagccc gaggagcgct tcggcgcgac gagcggcctc cgcggcgtct
     1741 tcggtatcgc cgagttcgta gaagtcgggg gtgggcacgc cgccgtgacg gacgatcttc
     1801 ttggtggcca ccttgttcat gccgagggcg gacgccagca cgcccgagcc cgtgtagggg
     1861 atgcccaaga tctcgagcaa cccctggagc gtcccgtctt cggcgtagcg accgtgcacc
     1921 gcgaggaacg cgacctcgac ccgctccgcc gtcagtcgct gagccacgtc gcgcagggcg
     1981 tcgatgcgca ccacgtcgta gcccagactt tgcagggcgc ggaggcagtt ctcgccggaa
     2041 cgcagggaca cctcgcgttc ccccgacaag ccccccatga ggacgccgat tctcttggac
     2101 ttcagatcca cgcgtgaact gtagcacagc ggccggaacc cccgagcggc ccgctgggcc
     2161 ggcccgagcg ccccggcgcg gcggcgcctt gggaggcccg ctgagagcca ggatctgtgt
     2221 gggagcatcg gcgggcgacc ggcccgccgc gagcagcgcg tgtt
//
LOCUS       DASRMJ010000341         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_432010, whole genome shotgun sequence.
ACCESSION   DASRMJ010000341 DASRMJ010000000
VERSION     DASRMJ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432010"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFD74_09515"
     CDS             <1..696
                     /locus_tag="VFD74_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZK49819.1"
                     /translation="DRMSLATGDLIIRILLSACLGGAIGLERELRDRAAGFRTHILVA
                     VGAAAFTIASVHGFDAFFTETAASNVRIDPGRIAAQVVSGIGFLGAGAIIRHGVSVRG
                     LTTAASLWAAAAVGLAAGLGLYVLAGVTAVTVVTSLYVLRFVEGRLIYPRVRDLLDMR
                     VTFRGRGFGPLTHLIDLLDHHHVAVQHMSVESDNVDANSIRLVLELPRGVTTAAAARL
                     VAELPDIESVSIN"
     gene            complement(765..2762)
                     /gene="uvrB"
                     /locus_tag="VFD74_09520"
     CDS             complement(765..2762)
                     /gene="uvrB"
                     /locus_tag="VFD74_09520"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZK49820.1"
                     /translation="MSLFSLRADYVPTGDQPEAIDRLVEGVERGDRFQTLLGVTGSGK
                     TFTMANVIARVGRPTLVIAHNKTLAAQLCNEFREFLPDNAVEYFVSYYDYYQPEAYIA
                     ATDTYIEKDSHINEEIDRLRHSATSNLLMRDDVVIVASVSCIYGLGSPQEYLRQVVLL
                     REGGEVDRDEVLAKLVAIQYERNDVGFARGRFRVRGDSFEVWPAYDDHVVRVSLFGDQ
                     VEEILRVDPITQEVLDRLPTVAIYPASHFVTGEGAIERAVREIAAELEQQVAHLEARG
                     KQLEAFRLRQRTQYDMEMLREVGYCSGIENYSRILDGREPGTAPHTLLDYFRDDFLVL
                     VDESHNTIPQLNGMYHGDRSRKLALVEGGFRLPSALDNRPLRFEEFLERVHQCVFVSA
                     TPAEWELSASSQVVEQLVRPTGLVDPVVEVRPTRNQIDDLMNEVRTRAERGERALVTT
                     LTKKMAEDLTDYLLEMGFRVRYLHSEVNTLERIQIVRDLRLGEFDVLVGINLLREGLD
                     LPEVTLVAILDADKEGFLRGETALVQTIGRAARNVEGRVIMYADRVTTAMQRAISETD
                     RRRGAQEAFNVAHGITPKSIIKGVSDITQLLGLSETVPFKEKRRLGRSGEALTEEEME
                     RLMTTLEEEMFAAAEELRFEYAAALRDEIKGLARDLQRLRS"
BASE COUNT          447 a          938 c          882 g          500 t
ORIGIN      
        1 gatcgcatgt cgctagccac cggtgatctc atcatccgca tactcctctc ggcgtgcctc
       61 ggaggcgcga tcgggctcga gagggagctg cgcgaccgtg cagccggctt ccgcacgcac
      121 atcctggtcg ccgtcggagc ggccgcgttc accatcgctt cggtgcacgg cttcgacgcc
      181 ttcttcaccg agaccgcagc cagcaacgtc cgcatcgatc cggggcgcat cgccgcgcag
      241 gtggtcagcg gcatcggctt cctgggtgcg ggcgccatca tcagacacgg ggtctcggtg
      301 cgcggcctca cgactgcggc cagtttgtgg gccgcggctg cggtaggcct ggcggccggc
      361 ctcggactct acgtgcttgc cggcgtcacg gcggtgaccg tggtgaccag cctctacgtg
      421 ctccgcttcg tggaagggcg actgatctac cctcgagtgc gtgatctcct ggacatgcgg
      481 gtgaccttcc gtgggcgtgg gttcggaccc ctcacccact tgatcgacct actggaccac
      541 caccatgtcg ccgtgcaaca catgagcgtc gagagcgaca acgtcgacgc gaactcgatc
      601 cgtctggtgc tcgagcttcc acggggcgtc accactgcgg cggccgcgcg tctcgtggcc
      661 gaactcccgg acatagagtc ggtcagcatc aactgaccgg ccgccaccgg ccgccgcggg
      721 aagcgcgccc ggcgggggcg ggactcgtgc cgccggcggg atcctcagct gcggagcctc
      781 tggaggtcgc gggcaagccc tttgatctcg tcgcggaggg ccgctgcgta ctcgaagcga
      841 agctcctcgg ccgccgcgaa catctcctcc tcgagcgtgg tcatcaggcg ctccatctcc
      901 tcctcggtca aggcctctcc gctccgaccc agccgacgct tctccttgaa ggggaccgtc
      961 tctgacaacc ccagtagctg agtgatgtcc gagacgccct tgatgatcga cttcggcgtt
     1021 atgccgtgcg ccacgttgaa cgcctcttgc gccccacggc ggcgatcggt ctcggagatg
     1081 gcccgctgca tggccgtcgt cacgcggtcg gcgtacatga tcacgcgccc ctccacgttc
     1141 ctcgcggccc ggccgatcgt ctgcaccagt gccgtctccc cgcgaaggaa tccttccttg
     1201 tcggcgtcaa ggatagcgac cagcgtcacc tccggcaggt cgagtccttc acgaagcagg
     1261 ttgatgccaa ccagcacgtc gaactcgccg agccgcaggt cgcgcacgat ctggatgcgc
     1321 tcgagagtgt tcacctcgga gtgcaggtag cgcacgcgga accccatctc gagcaggtag
     1381 tccgtgagat cctccgccat cttcttggtc agggtggtga ccagcgctcg ttctccgcgc
     1441 tcggcgcgag tgcgcacctc gttcatgagg tcgtcgatct gattgcgggt gggccgcacc
     1501 tcgacgaccg gatcgacgag acccgtgggc cgcaccagtt gctccaccac ctgcgaagag
     1561 gctgacagct cccattccgc cggagtcgcc gacacgaaaa cgcactgatg cacccgttcc
     1621 aagaactcct caaagcgcag cgggcggttg tcgagagcgg acggcagacg gaacccccct
     1681 tccacgagcg cgagcttgcg gctgcggtcg ccgtgataca tgccgttgag ctgcgggatg
     1741 gtgttgtgcg attcgtccac caacacgagg aagtcgtcgc ggaagtagtc gaggagcgtg
     1801 tgaggcgccg tgcctggttc cctaccgtca aggatgcgcg agtagttctc gatgcccgag
     1861 cagtacccga cctcgcgcag catctccatg tcgtactgcg tgcgctgccg tagcctgaag
     1921 gcttcgagct gcttcccccg cgcctccagg tgagcgacct gctgctcgag ttcggccgcg
     1981 atctcgcgca cggcgcgctc gatcgccccc tcccccgtga cgaagtgcga agcggggtag
     2041 atcgccaccg tcggcagccg atcgagcacc tcttgcgtga tggggtcgac ccgcagtatc
     2101 tcctcgacct ggtcgccgaa caggctcacg cgcaccacgt ggtcgtcgta ggcgggccac
     2161 acctcgaaac tgtcgccgcg cacgcggaac cgcccgcgcg cgaagcccac gtcgttgcgt
     2221 tcgtattgga tcgccacgag tttggccaag acctcgtcac gatcgacctc tcccccctcc
     2281 cgcagaagga ccacctgccg caggtactcc tgcggtgaac cgagcccgta gatgcaggag
     2341 accgaggcga cgatcaccac gtcgtcgcgc atgaggaggt tactcgtggc cgaatggcgc
     2401 agccggtcga tctcctcgtt gatgtgggag tccttctcga tgtaggtgtc ggtggcggct
     2461 atgtaggctt ccggctggta gtagtcgtag taggagacga agtactccac cgcgttgtcg
     2521 ggcaggaact ctctgaattc gttgcagagt tgagctgcca gcgtcttgtt gtgcgcgatg
     2581 acgagggtgg gccgacccac gcgggcgatg acgttcgcca tggtgaaggt tttccccgat
     2641 cccgtcacac cgaggagcgt ctgaaagcga tcgccacgct cgacaccttc gaccagccga
     2701 tcgatggcct cgggctgatc gccggtgggg acgtagtcag cacgaagtga gaacagactc
     2761 acagagc
//
LOCUS       DASRMJ010000342         9062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_250752, whole genome shotgun sequence.
ACCESSION   DASRMJ010000342 DASRMJ010000000
VERSION     DASRMJ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250752"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(453..1808)
                     /locus_tag="VFD74_09525"
     CDS             complement(453..1808)
                     /locus_tag="VFD74_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZK49821.1"
                     /translation="MTSISEKALAITAREKERYANMTPGSQKATERAKKVLPLGVASN
                     FQFFEPHPIVADHASGSRLWDVDGNEFIDYNGGYGSLFAGHSHPTLVEAITAQAPKGT
                     LFTTPSVLIAEVAEALVERFQFEMVRFTNSGTEATMDALRLARGYTGREKIIKLEGGY
                     HGHHDLVMMSVRPPLDKAGPADHPNTVPYYGGTAKATIDEVVVISFNDLPALERAFTE
                     HPGEIAAFILEPVPENMGIVLPDDGYLAAAKEICHKHGALIIFDEVKTGITSNWGGAS
                     TIYSVQPDIICLAKSIGGGVPIAAFGASREIMGYIADFTVAHQGTFNGNPLVTAASLA
                     VLTKICTKEATAAAIARNARLIEGCQKVIDETGLPAHTVQLGAKGCVTYVPERVRTFR
                     DYKKTNFELAYAHWIYMMSHGIFLPPGLDEQWLVSVAHTDADIDRHLEIFEAFVREVT
                     A"
     gene            complement(1805..2317)
                     /locus_tag="VFD74_09530"
     CDS             complement(1805..2317)
                     /locus_tag="VFD74_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZK49822.1"
                     /translation="MTAKLDDIDREIIALLLQDGRMSSTQMARRLGDVSERTVRYRVD
                     RLIDRGVIQIGAIVNPRSLGYAVTADVWIEVSPSRLREVAEQLAHLQEVSYVAYSTGD
                     RDLSIQVYARDNEELHAFVSDVVGNLPGVTRTSIMLVPWKVKDVHQWRVPDNAPVNRK
                     VAGENARRRA"
     gene            complement(3005..4912)
                     /locus_tag="VFD74_09535"
     CDS             complement(3005..4912)
                     /locus_tag="VFD74_09535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK49823.1"
                     /translation="MADSHWQARYEAKRTTVRDALSNIRRGDRIFIGAGCGEPQSLVR
                     GLAEMGSKLVDSEVYHLLTLGTAPYTAPRFSDTFRHNSFFIGHDVRSDVAEGRADYTP
                     VFLSEVPALFTDGHIPVDVALIQVSPPDAHGTCSYGVAVDITKPAAESARIRIAQVNR
                     LMPRTLGDSFIHVDDLNVVVELDEPILTVPLHETTEVSREIGRQVAKLVDDGCCLQVG
                     IGSVTGAMLDHLHNRRDLGLHTEMFTDAVIDLISEGVINNSLKTLHRGKILASFCMGT
                     HRLYEFVHDNPMCEFRPSSYTNDPYIIGQNDKMVAVNAALEIDLTGQVCSDSLGYDFY
                     SGIGGQVDFMRGAARSRGGKPVICLPSEAAEGRSRIVPHLSEGAGVVTTRGGVHYVVT
                     EHGIAYLHGKSIRERAMALIQIAHPDHRAELLAHAKERRYIFMDQESPRSDRYPDELE
                     RYVTLDDGGTVLFRPLRPTDDALVRDLFYDTSEDAVYKRFMNAKATYPRDERDAIVNI
                     DYRTTMSLAAARSGPGAAELVALAEWRLSPDDDYAEVAFMVRDDWQHRGLGMRLLQYL
                     IELGTERGLAGFRAEVLPDNQGMLHVFQQCAREVKIKLVDGAYEVLIDFATADARTPP
                     GTSAPPPEAAE"
     gene            complement(5104..7062)
                     /gene="acs"
                     /locus_tag="VFD74_09540"
     CDS             complement(5104..7062)
                     /gene="acs"
                     /locus_tag="VFD74_09540"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZK49824.1"
                     /translation="MANEVSLEGKVFDVPASFQKHAWISSMDQYNEMYRRSVDESDAF
                     WAEVAERITWFKKWDKVQDHDFKNAKIRWFDGGKLNLSYNCLDRHLETEAADKVAFYW
                     EGDSPEVSRTITYRQLWAEVAKFANVLRKKGVKKGDRVTIYLPMIPELPVAMLACARI
                     GAIHSVVFGGFSSDSLRDRILDCESTIMITADGGLRGSKNVPLKAAADEAMAACPSMN
                     TCIVVRHTGLGVDWDEARDSWYHEEMSADDITTDSPCEEMDAEDPLFILYTSGSTGKP
                     KGVMHTTGGYAVYTSYTHELVFDYHPEDVFWCTADIGWVTGHSYIVYGPLANGATSVM
                     FEGVPNYPAPDRFWAVVEKYKVTQFYTAPTALRAIAREGDDWVTKHDRSSLRLLGTVG
                     EPINPEVWLWYHGLVGEGRCPIVDTWWQTETGGILITPLPGAMKIKPGSATLPFFGVE
                     PVLVDADGVEVEGPGVGNLCIRRPWPGQMRGVYGDTNRFFDTYFKQYPGLYFTGDGCR
                     RDEDGYYWITGRVDDVINVSGHRMGTAEVESALVSHDSVAEAAVVGMPHDIKGQGIYA
                     YVTLNVGVEPSDELKKMLVAHVRKEIGPIATPDVIQWAPGLPKTRSGKIMRRILKKIA
                     ANDVSDLGDTSTLADPTVVDNLVANRSV"
     gene            complement(7380..8702)
                     /locus_tag="VFD74_09545"
     CDS             complement(7380..8702)
                     /locus_tag="VFD74_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HZK49825.1"
                     /translation="MPALEPLDAHEKGVVASIREARDEIVALASELISYDTTARDPGA
                     PARDEAFLQGALARRLETAGATVDLWEPEPTAPDSRFVPPTLDFRGRPQMAACFRGTC
                     GGRSLMLNGHIDAVDPGDISLWTSPPLSPEVRGGRLYGRGAADMKGGIASFVLAAEVL
                     ARLGVRLAGDLIVSTNTDEESSGAGGFALVQRGIRADACMVAEPTGFDAWVSCRGTVT
                     PTIVVDGRAGHAEMRQPHWRDGGAVNAIEKMGLVLDAIRSLREEWRDRQDQKHPLLPP
                     GDIVPTIIHGGNWMVTYPERCEMVVDISYLPTHVDAAGTGRAVELEVMEWIQAAAAAD
                     PWLARHSPRFHWTVDVVPAEIAADHPLVTLALGAGSVLGRPGKISGLDSWHDAATFTR
                     WGHTPSFSFGPGGMHTAHAVDEYVPVEDLVDLAAAAALTAMRWCGVVD"
BASE COUNT         1488 a         2799 c         3108 g         1667 t
ORIGIN      
        1 attgccggga ctgcagaagt acctgcatag cgccgtgatc gtcaaggaga tcgtggttcc
       61 ggggaaactg gtcaatctgg tggtgcgccc tgagtgatcg ggcgccctgc gcgacgtgcc
      121 ccggcgctgt acagtcttgc cttgctgaag tacactggtg ccggtctgag ggcacctcga
      181 accacactat cgggcgtgtc tccgagaccc agaccagcgg cgcttgtgag ggtcgagtcg
      241 ctcaccattc cttcgaattg ggggtaacaa catgagagtc cgtcccgttc ttcgatcgtg
      301 tcgctgagcc cactgccgct cctgtcggta ggtcccggac gggcctgacc gcccgggacc
      361 tggtctcacg tggttctgcc ggtgcatcgc gcggccgccc tgcggccaag accaggcccc
      421 gctcggagcg ggggcctggt cgggtgatca gatcaggcgg tgacttcgcg cacgaaggcc
      481 tcgaagatct cgagatgccg gtcgatgtcc gcgtcggtgt gggccaccga taccagccac
      541 tgctcgtcga ggcccggggg caggaagatc ccgtggctca tcatgtagat ccagtgagcg
      601 taggcgagtt cgaagttggt cttcttgtag tcgcggaagg tgcgcactcg ctccggcaca
      661 taggtgacgc agcccttggc gccgagttgc accgtgtgcg ccggcaggcc ggtctcgtcg
      721 atcaccttct gacaaccttc gatcaggcgg gcgttgcggg cgatggcggc tgcggtggcc
      781 tctttggtgc agatcttggt gagaacggcc agcgacgcgg cggtgacgag ggggttgccg
      841 ttgaaggtgc cctgatgggc cacggtgaag tccgcgatgt accccatgat ctcccggctc
      901 gcgccgaacg cggctatggg tacgccaccg ccgatcgact tagcaagaca gatgatgtcc
      961 ggctgcaccg agtagatcgt cgacgcaccg ccccagttcg aagtgatgcc ggtcttgact
     1021 tcgtcgaaga tgatgagagc gccgtgcttg tggcagatct ccttggctgc ggcaaggtag
     1081 ccgtcgtccg gcaggacgat gcccatgttc tccggcaccg gctcgaggat gaacgccgcg
     1141 atctcgcccg ggtgctcggt gaaggcgcgc tcgagcgcag gcaggtcgtt gaaggagatg
     1201 accactactt cgtcgatcgt ggctttggcc gtcccgccgt agtaggggac ggtgttcggg
     1261 tggtcggccg ggcctgcttt gtcgagcgga ggacgcaccg acatcatcac caagtcgtgg
     1321 tgaccgtggt agccgccttc gagcttgatg atcttctcgc ggccggtgta gccgcgggcg
     1381 agcctcaggg catccatcgt ggcttccgtg cccgagttgg tgaagcgcac catctcgaac
     1441 tggaatcgtt cgacgagagc ctcggctact tcagcgataa ggaccgaagg agtggtgaag
     1501 agggtgccct tcggagcctg ggctgtgatt gcctcgacca gggtcgggtg ggagtgtccc
     1561 gcgaacaggg acccgtaccc gccgttgtag tcgatgaact cgttgccgtc gacgtcccag
     1621 agtctgctgc ctgacgcgtg gtcggcgacg atggggtgtg gttcgaagaa ctggaagttg
     1681 ctggcgacgc cgagaggaag caccttcttg gcccgctctg tggccttctg gctccccggc
     1741 gtcatgttgg cgtaccgctc cttctcacga gcggtgatag ccaaagcctt ctctgagatg
     1801 ctcgtcatgc tctcctcctt gcgttctcgc cagcgacctt gcggttcacc ggcgcattgt
     1861 ccgggacgcg ccactggtgc acgtctttga ctttccacgg cacgagcatg atgctcgtgc
     1921 gggtgacccc gggcagattc cccacgacgt cactgacgaa ggcgtgaagc tcctcgttgt
     1981 cgcgcgcgta cacttggata cttaagtctc ggtcgcccgt gctgtaggcc acgtagctga
     2041 cctcctgaag atgtgccagt tgctcggcga cctcgcggag tctgctggga gagacttcga
     2101 tccacacgtc ggccgtgacg gcgtatccga gagaccgggg gttgacgatg gcgccgatct
     2161 ggatgacgcc tcgatcgatc agcctgtcga cccggtagcg caccgtgcgc tccgagacat
     2221 cgcccagtcg ccgagccatc tgcgtgctgg acatgcgtcc gtcctgcaag agaagcgcga
     2281 tgatctcccg atctatgtcg tcgagcttgg cggtcacgag tcctccggtg cgtgcgtgaa
     2341 tacagtatat gcacaagtgt gccgaataag tcaaacttgg cggcgcaact ggccgatacg
     2401 gcaacatgga aggagtgcat agaacgagtg cgcgtgctct tcgggcgcaa agacggtagg
     2461 accgggtgcc cacgcgtggg cgacgcgagg ggcgtccccc gcggacggaa ctcaggccgt
     2521 cctggttctc cggccgggaa ccacggcttg atgagggcag tactgagcgc acagcaggca
     2581 gccggtgcag cggtcctcgt ccacggtcca cgcggtcgga gtcggtggag caagcagtcc
     2641 gcgaagccag gaagtctcag gtgcccgtag gtccgaatcc gggataaccg cctgacgagg
     2701 gcagatgcgg ctgacggggc agcctggagc accgtcgcag cgcgcgaggt cgatgctcgc
     2761 taccggcttg tcctcgcgcc gtgtcctgcc gccttcgggg tgctggtttc tcgtcttctt
     2821 gcgtgcggcc gacatgtgcg ctccatttta cgatcgaggt gggaaccacc cgtgacgcca
     2881 agggtccggg ggtggtcgtc ggatgtaccg gtcggcatct gatacccacg ggggtatgga
     2941 atggcttcag tctacactga tggcgctctc tcgacaagcg cccgccccgc ccgcgtatgg
     3001 ccgtctattc ggccgcttcg gggggcgggg cggaggtgcc cggaggggtg cgcgcgtcgg
     3061 cagtggcgaa gtcgatgagc acctcgtagg cgccgtccac caacttgatc ttcacctctc
     3121 gcgcgcactg ctggaagacg tgcagcatcc cctggttgtc gggcaggacc tcggccctga
     3181 agccggccag gcctcgttcc gtgccaagct cgatgaggta ctgcagcagg cgcatcccca
     3241 ggccccggtg ctgccagtcg tcgcgcacca tgaaggcgac ctcagcgtag tcgtcgtcgg
     3301 ggctgaggcg ccactcagcc aaagcgacca gctcggctgc gcccgggccc gagcgggcag
     3361 ccgccagact catcgtggtg cggtagtcga tgttgacgat ggcgtcgcgc tcgtcgcgcg
     3421 ggtaggtggc cttggcgttc atgaagcgct tgtagacggc atcctcgctg gtgtcgtaga
     3481 acagatcccg caccaaggca tcgtcagtcg gacgcagagg acggaagagt accgttccgc
     3541 cgtcgtcgag ggtcacgtag cgctccagtt cgtccgggta tcgatcgctg cgtggggatt
     3601 cctggtccat gaagatgtag cgtcgctcct tggcgtgagc cagcagttcg gcgcggtggt
     3661 ccgggtgggc gatctgtatc agagccatgg ctcgctcgcg gatggacttg ccgtggaggt
     3721 aggcaattcc gtgctcggtg accacgtagt ggactccgcc tcgggtggtc accactccgg
     3781 ctccttcgga gaggtgcggg acgatccggc ttctaccctc cgccgcttca gaaggcaggc
     3841 aaatgacggg cttgcctccg cgggatctcg cagctccgcg catgaaatcc acctggccgc
     3901 cgatgccgct gtagaagtcg tagccgagag agtcggagca gacttggccg gtcaagtcga
     3961 tctccagcgc ggcatttacg gccaccatct tgtcgttctg gccgattatg taggggtcgt
     4021 tggtatagct ggaggggcgg aactcacaca tggggttgtc gtggacgaac tcgtacaagc
     4081 ggtgggtccc catgcagaac gaggcgagga tcttgccgcg gtggagggtc ttgagcgaat
     4141 tgttgatcac gccttcggag atcagatcga tcaccgcgtc ggtgaacatc tccgtgtgga
     4201 gccccaagtc tcgcctgttg tgcaggtgat cgagcatggc gcccgtcacg gagccaatcc
     4261 ccacctggag acagcagccg tcgtccacca gtttggctac ttggcggccg atctcgcggg
     4321 agacctcggt ggtctcgtgc agcgggaccg tgaggatcgg ctcatcgagt tccacgacga
     4381 cgttcaaatc gtccacgtgg atgaacgaat ctcccaacgt gcggggcatg agccggttca
     4441 cctgggcgat gcggatacgc gcgctctcag ccgcgggctt cgtgatgtcc acggctacgc
     4501 catacgagca cgtgccgtga gcgtcgggag gggatacttg gatgagggcg acgtccacag
     4561 ggatgtgccc atcggtgaac agcgcgggca cctccgaaag gaacaccggc gtgtagtcag
     4621 ctcgaccctc agccacgtcg gagcgcacat cgtgtccgat gaagaaggag ttgtgacgga
     4681 aggtgtcgct gaagcgggga gccgtgtaag gggcggttcc cagggtgagc aggtggtaga
     4741 cctccgaatc gacgagcttc gagcccatct cggcgagccc acgcaccaag gattgcggct
     4801 cgccgcaccc tgcgcctatg aagatgcggt cgcctctccg gatgttggat agggcgtccc
     4861 gcacagtcgt gcgcttggcc tcgtagcggg cctgccaatg actatccgcc atcacctctc
     4921 ccgtgccgtc gagtcacaac gaagtcgttg ctcccagaac atacaccaag aagtcgacgg
     4981 gtatggggtt gaacggagag ggcggccggg gccgccctct ccgttcagtc tcccaagagc
     5041 cgggatggac cggactctct cggtatagtc tcgtccgcag gcgtctcgag tcgggtcgtc
     5101 cggtcagacg ctccggttgg cgacgaggtt gtccaccacg gtggggtcgg ccaacgtcga
     5161 ggtgtcgccc agatcgctga cgtcgttggc cgcgatcttc ttcaagatgc gccgcatgat
     5221 cttgccgctg cgtgtcttgg ggagtccggg ggcccactgg atgacgtcag gtgtggctat
     5281 cgggccgatc tccttgcgga cgtgtgcgac gagcatcttc ttgagctcgt ccgacggttc
     5341 gacgccgacg ttcagcgtga cgtaggcgta gattccctgg cccttgatgt cgtgaggcat
     5401 gccgaccacg gctgcttcgg cgacggagtc gtgactcacc agggccgact cgacctcggc
     5461 ggtgcccatg cggtgcccgg acacgttgat gacgtcgtcg acgcgccccg tgatccaata
     5521 gtagccgtct tcatcgcgcc tacaaccgtc accggtgaag tacagcccgg ggtattgctt
     5581 gaagtaggtg tcgaagaagc ggttggtatc gccgtataca ccgcgcatct gaccgggcca
     5641 gggccggcgg atgcagaggt tgcccactcc gggcccctcg acctcgacgc cgtcggcgtc
     5701 gaccaacacg ggttccacgc cgaagaacgg cagagtggcc gagccgggct tgatcttcat
     5761 ggccccgggg agcggcgtga tgaggatgcc gccggtctcg gtctgccacc aggtgtcgac
     5821 gatggggcag cgtccttcgc ccaccagacc gtggtaccag agccacactt ccgggtttat
     5881 gggctctccc accgtcccaa gaaggcggag tgaggaacgg tcgtgtttcg tgacccaatc
     5941 gtcgccttcg cgggcgatgg cgcggagggc tgtaggggcg gtgtagaact gggtgacttt
     6001 atacttctcc accaccgccc agaagcggtc gggcgcgggg tagttgggca ctccctcgaa
     6061 catgacggat gtagcgccgt tggccaacgg gccgtagacg atgtagctgt gcccagtgac
     6121 ccagccgatg tcggcggtgc accagaagac gtcttcggga tggtagtcga agaccagctc
     6181 gtgtgtatag ctcgtgtaga cggcgtagcc gcccgtagtg tgcatgacgc ccttgggctt
     6241 gccggtggac ccggaggtgt agaggatgaa cagaggatct tcagcgtcca tctcttcgca
     6301 cggcgagtcg gtggtgatgt cgtcggccga catctcctcg tgataccagg agtcgcgcgc
     6361 ttcgtcccag tcgacgccca ggccggtatg gcgcacgacg atgcaggtgt tcatgctggg
     6421 gcaggcggcc atggcctcgt cggcggcggc cttcagcggg acgttcttgg acccgcgcaa
     6481 cccgccgtcg gcggtgatca tgatggtcga ctcgcagtcg aggatgcggt cgcggagaga
     6541 gtcggacgag aagccgccga aaaccacgct gtggatggca ccgatgcggg cgcaggccaa
     6601 catggccacc ggtagttcgg ggatcatcgg gagatagatg gtgacgcgat cgcccttctt
     6661 cacacccttc ttgcgcagga cgttcgcgaa cttcgccact tcggcccaca gttggcgata
     6721 ggtgatggtg cgggatacct ccggggaatc accttcccag tagaaggcca ccttgtccgc
     6781 cgcttcggtc tcgaggtgac ggtcgaggca gttgtagctc agattgagct tgccaccgtc
     6841 gaaccagcgg atcttggcgt tcttgaagtc gtggtcttgg accttatccc acttcttgaa
     6901 ccaggtgatg cgttcggcga cttccgccca gaaggcgtcg gattcgtcga ccgaccttcg
     6961 atacatctcg ttgtactggt ccatgctcga gatccaggcg tgcttctgaa agcttgcagg
     7021 cacgtcgaag accttgccct cgagagagac ttcattggcc acgttcaacc tccgcagata
     7081 gttgtcactg gctcgccccg gccagcttca agggttgata cggcaggcgg attcaaagaa
     7141 accatacagg cgggcgcggg tcgaggcaat gagaccttgg atgtttccgc gagcggccga
     7201 tagcgttccg cgaccggccc tcaccggggg aggagcggcg tccgggagtg ctgcgcgttc
     7261 gcgcttcggg cccggagacg gcgccatgcg gggacacgtg acgatgccgc cgccggaccc
     7321 gacggtgccg gacccgacgg cgcccgactc gacggcgccc gacccgacgg cgcccgcgct
     7381 cagtcgacca ccccgcacca tcgcatcgcc gtcagtgccg cggcggcggc aagatcgacg
     7441 agatcctcta ccggtacgta ttcgtccacc gcgtgcgccg tgtgcatgcc gccggggccg
     7501 aaggagaagc tgggagtgtg gccccagcgc gtgaaggtgg ccgcgtcgtg ccaggagtcg
     7561 aggccgctga tcttgcccgg ccggccgagg accgagcctg cgccgagggc gagtgtcacg
     7621 agcgggtggt ccgcggcgat ctcggcgggg acgacatcga ccgtccagtg gaagcgggga
     7681 gagtgccggg ccaaccacgg gtccgccgca gcggcggcct ggatccactc catcacctcc
     7741 agctcgacgg cacggccggt gccggcggca tcgacatggg tgggcagata ggagatgtcg
     7801 acgaccatct cgcatcgttc gggataggtg accatccagt tcccgccgtg gatgatcgtg
     7861 ggcacgatgt caccgggagg gaggagaggg tgcttctggt cttggcggtc tcgccactcc
     7921 tcgcggaggg accggatggc atcaaggacc aatcccatct tctcgatggc gttcaccgcg
     7981 ccgccgtcgc gccagtgcgg ttggcgcatt tccgcgtggc ccgcccgccc gtcgacgacg
     8041 atcgtggggg ttaccgtgcc gcggcaggag acccaagcgt cgaaacctgt gggctccgcc
     8101 accatgcatg cgtcggcgcg gatcccccgc tgcacgagcg cgaacccccc ggcgcctgaa
     8161 gactcctcgt cggtgttcgt cgatacgatg agatccccgg cgaggcggac gccgagcctg
     8221 gcgagcacct cggcggcgag cacgaaagac gcgatgccgc ccttcatgtc ggccgcgccg
     8281 cgaccgtaga ggcggccgcc tcggacttcg ggcgacagcg gaggcgaggt ccacaaggag
     8341 atgtcgcccg ggtccacggc atcaatgtgg ccgttgagca tgaggctccg tccgccgcag
     8401 gtccctcgga agcacgcagc catttgcggc cgaccccgga aatccaacgt gggcgggacg
     8461 aagcgtgagt cgggagcggt cggctcgggt tcccagaggt cgacggtggc gcctgcggtc
     8521 tcgaggcgcc gcgccagcgc tccttggagg aaggcctcgt cgcgcgccgg ggcacccgga
     8581 tctcgggccg tcgtgtcgta ggagatcagc tccgaagcca acgccacgat ctcgtcgcgc
     8641 gcctctcgga tcgaggctac gactcccttt tcgtgcgcat cgagtggttc cagggcgggc
     8701 atccgatcct cctgcgcggt cgtggcgagt ggcggttcca agctatcacc cgcgcacgac
     8761 cgcgtcgatg agatctcgac actgactgca ggaggcgttc gcgacctggg cgcctgcggg
     8821 tgcatcggag tggcatcggg ggtttccgag ggccaccggg gatccgcagg cgcgtgggcc
     8881 ggggcccgcc tatgcgtgga agagaacggc cgggcacccc tgcggggtag cccggccgtt
     8941 cgacaaggtg ctgtgcgctc gagagcgccc gactgctgtc agatccgagg tccggctagt
     9001 tgccccagcc gcccatcatg ctaccggcgg agccgccgcc catcatgccg ccgccccagc
     9061 cg
//
LOCUS       DASRMJ010000343         5106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_281918, whole genome shotgun sequence.
ACCESSION   DASRMJ010000343 DASRMJ010000000
VERSION     DASRMJ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281918"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="VFD74_09550"
     CDS             <1..119
                     /locus_tag="VFD74_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010614264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZK49826.1"
                     /translation="VDLIVIGRRGLGKFSGLLLGSTSDRVLHYATVPVLVAH"
     gene            355..1587
                     /locus_tag="VFD74_09555"
     CDS             355..1587
                     /locus_tag="VFD74_09555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HZK49827.1"
                     /translation="MKQLLVIGAGTAGTMAVNRLRRALDADEWKITIVDPKETHYYQP
                     GFLFIPFGMYSKNDVVKPKRDFIPVGVEMIKAAADVIEPDHNQVKLSDGRILHYDFLV
                     IATGTQTRPEETPGLMESEWQRSIFDFYTVEGAVALAKHLRTWQGGRLVVNVVENPIK
                     CPVAPLEFIMLADWFFHEQGIRDKVELIYATPLPGAFTKPIASKHLGDILEQKSVEVI
                     PEFMVERVDPEAKKLVHYDESEVGYDLLVTIPLNMGDDLIARSGLGDELNYVPVDKHT
                     FLSDAHENIFALGDAAALPTSKAGSVAHYAVDCFCENFVRYIDGLEMMPTFDGHANCF
                     IESGFGKGLLIDFNYDVEPLPGHFPLPGVGPFSLLQESEMNHWGKMMFRWIYWNTLLK
                     GKDLPLPARMSMSGKRRT"
     gene            1598..2275
                     /locus_tag="VFD74_09560"
     CDS             1598..2275
                     /locus_tag="VFD74_09560"
                     /inference="COORDINATES: protein motif:HMM:NF019464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HZK49828.1"
                     /translation="MEQTPVTLDQKLDRLAEQVQYLSDQAQCSARRQEARAELLDDAT
                     PIINDVFRITTEQLEEVQDYVDLSDLLRLLKRLMRNTRNLDAMLDQLESLSDLGQTVG
                     PLVGDAFDRTTDLLEAAENKGYFTFAKGGVKVMDNVVTSFGEEDLGHLGDNIVLILSV
                     IKDMTQPQILSLVRAIISEGEVEIAQPVNTSLPALLKQMRDPDVRRGFGLTMRVLGII
                     GAQVPGK"
     gene            2352..2672
                     /locus_tag="VFD74_09565"
     CDS             2352..2672
                     /locus_tag="VFD74_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HZK49829.1"
                     /translation="MATRSIAGIEVQVNDEGYMTDPAEWTKDIAVEIAGEENLGELTD
                     AHWRVIDFCRDSAQKLNGKSPTLRQITTGTGVTTKELFGLFPKGPAKKVARIAGLGKP
                     EGCV"
     gene            2732..3232
                     /locus_tag="VFD74_09570"
     CDS             2732..3232
                     /locus_tag="VFD74_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HZK49830.1"
                     /translation="MTEENRHIAFICSKGALDMAYPALVMGWAALGNGIDVTIFFTFW
                     GMDIINEKRVDHLEIAPVANTSMKMGMMGVPGNLGIPNLMGVLPGMTAFSTSLMKKKM
                     TEVGAPPVREYLQMLVDGGAKLYACKMSADMMELKMEDFVDGVIDIVTASDFMDLTDG
                     AQMVFI"
     gene            complement(3341..3796)
                     /locus_tag="VFD74_09575"
     CDS             complement(3341..3796)
                     /locus_tag="VFD74_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZK49831.1"
                     /translation="MSGPRDISAGDESWPGAGQPCFLDTSALYAVFDRAQVRSAEVVA
                     VWERLVRSDVSLLTTNYVLLELTALLQRRLGPDAVGAFQDFVLPWLKVVWVDEAVHGT
                     ALALVRGARRRDISLVDAVGFAVMRSRGSRHAFTLDGHFAEQGFTCLPG"
     gene            complement(3793..4041)
                     /locus_tag="VFD74_09580"
     CDS             complement(3793..4041)
                     /locus_tag="VFD74_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZK49832.1"
                     /translation="MIRTQIQLHDDQARRLREIAAAEEVSLAEMIRRGVDMVLESSSE
                     LTRRDRLDALRVAGSFDSGLPHVAERHDDELAEAYLGQ"
     gene            complement(4165..>5106)
                     /gene="mch"
                     /locus_tag="VFD74_09585"
     CDS             complement(4165..>5106)
                     /gene="mch"
                     /locus_tag="VFD74_09585"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR03120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HZK49833.1"
                     /translation="AIVERMIARSAELGIAAHRLGNGATVIDCGARTAGSFEAGRLYA
                     EACMGGLGDVRLKVAELGGWSTPAVEVDVRHPLLACMGSQLAGWAVEVPPQGDAPGFY
                     ALGSGPARALCRREPIFAIIPHVEVAQAAVLSMETWDLPGDRAADWIAERCCRLSAEG
                     LYLLVAPTASLAGSVQVAARVVETALYKLHRSGFDLTTVISAFGSCPVAPVAADDLVA
                     VGRTNDGIIYGGRAWLSVTCEDAAVEAVLDMLPPSASPDWGVPFGSLYERACADFYQL
                     DPLLFSPAEMWITNTRSGRTYHVGRLDPEIVRASFLG"
BASE COUNT         1025 a         1650 c         1628 g          803 t
ORIGIN      
        1 acgtagatct gatcgtcatc gggcgccgcg gccttgggaa gttctcgggg ctgctcttgg
       61 gttccacctc ggaccgggtg ttgcactacg ccaccgtgcc ggtgttggta gcgcactgac
      121 cagcggcgac acacagccgg accatccccg tgcggtgagt gagaccagct agatctgcca
      181 cttgcaccct ttataccccg tagggtatga tgggtccgca cgacgcatct ccccgcacaa
      241 cgcatcctcc acccggagtt gcgggacact tgtggggtcg aaccgtgcac gtatgtgatt
      301 ggcgtcacaa tcgacgcggc ataggcgcag cgcactacag ggaggttctc gtacatgaag
      361 cagttgctgg tgatcggcgc cggaacggcg ggcacgatgg ccgtcaaccg actccgccgg
      421 gccctggatg ccgacgagtg gaagatcacc atcgtcgacc ccaaagagac gcactactac
      481 caacccggct tcctcttcat cccctttggc atgtacagca agaacgacgt ggtgaagccg
      541 aagcgcgact tcatccccgt cggtgtcgag atgatcaagg cggcagccga cgtcatcgag
      601 cccgaccaca atcaagtgaa gctcagcgac ggtcggatac tccattacga cttcctggtg
      661 atcgccaccg gcacacagac gcggcccgaa gagaccccgg gcctgatgga gtccgagtgg
      721 cagcgctcca tcttcgactt ctacacggtg gaaggcgcgg tggcgctggc gaagcatctg
      781 cgcacctggc agggcggcag gctggtggta aacgtcgtgg agaaccccat caagtgcccg
      841 gtcgcgccgc ttgagttcat catgctcgcc gactggttct tccacgagca aggcatccgc
      901 gacaaggtgg aactgatcta cgccactccc ctcccgggcg ccttcacgaa gcccatagcc
      961 tccaagcatc tgggcgatat cctcgagcag aagagcgtcg aggtcatccc cgagttcatg
     1021 gtggagcggg tcgaccctga agccaagaag ctcgtgcact acgacgagag cgaggtggga
     1081 tacgacctgt tggtgacgat cccgctcaac atgggcgatg acctcatcgc gcgctccggg
     1141 cttggcgacg agttgaacta cgtgccggtg gacaagcaca ccttcctgtc cgacgcgcac
     1201 gagaacatct tcgccctggg cgacgcggcg gccctaccca cgtccaaggc aggatccgtg
     1261 gcccactacg ccgtggactg cttctgcgag aacttcgttc gctacatcga cgggctggag
     1321 atgatgccga cgttcgacgg tcacgcgaac tgcttcatcg aatccggctt cggcaagggg
     1381 ctcttgatcg acttcaacta cgacgtcgaa cccctccccg gacacttccc gctgccaggg
     1441 gtgggtccgt tctccttgct gcaggagtcc gagatgaacc actggggcaa gatgatgttc
     1501 cgctggatct actggaacac cctgctcaag ggcaaagacc tgccactgcc cgctcgcatg
     1561 agcatgtcgg gcaagcggcg cacgtagggg gcgagggatg gaacagacac ccgtcactct
     1621 cgaccagaag ctcgaccggc tcgccgagca ggtccagtac ctgtccgacc aggcgcaatg
     1681 ttctgcccgc aggcaggagg cgcgcgccga gctgctcgac gacgcgacgc ccatcatcaa
     1741 cgacgtcttc cgcatcacca cggagcagct ggaggaagtg caggactacg tcgacctgtc
     1801 tgacctgctg cgcctactca agcgtctcat gcgtaacacc cgcaacctgg acgcgatgct
     1861 cgaccaactc gagagcctgt ccgacctcgg gcagacggtc ggccccctcg tcggcgacgc
     1921 cttcgacagg actactgacc ttctcgaggc ggccgagaac aagggctact tcaccttcgc
     1981 caagggtggg gtgaaggtca tggacaacgt ggtcacgtcc ttcggagagg aagacctcgg
     2041 gcacctgggc gacaacatcg tgttgatcct gagcgtgatc aaggacatga cccagcctca
     2101 gatcctgagc ctcgtccgcg cgatcatctc tgagggagaa gtcgagatag cgcagccggt
     2161 caacacgtcg ctgccggcgt tgctgaagca gatgcgtgac ccggatgtgc gtcggggctt
     2221 cgggctcaca atgcgggtgc tcggcataat cggcgcgcag gtgcccggta agtagggaca
     2281 tcacccgtgc cggcgagact ccgcgcgggg acgacaacgg caagacaacc gaacgacagg
     2341 aggaaggccg agtggcgacc agatcgatcg ctggtataga agtgcaggtg aacgacgaag
     2401 ggtacatgac ggacccggcc gagtggacca aggacatcgc agtcgagata gccggggaag
     2461 agaacctcgg cgaactgacc gacgcccatt ggagggtgat cgacttctgc cgcgattcgg
     2521 cgcagaaatt gaacgggaag tcgcccacgc tgcggcagat caccaccggg accggggtga
     2581 cgaccaagga gctgttcggc ctgttcccaa agggcccggc gaagaaggtg gcccgcatag
     2641 ccggcctggg caagcctgaa ggctgtgtct agccgtctag cggctctcag caaccccaca
     2701 cgacagaaga agcccacagg aggcatacca catgaccgaa gagaaccgac acatagcgtt
     2761 catctgctca aagggcgccc tcgatatggc gtaccccgca ctggtcatgg gctgggcggc
     2821 cctgggcaac ggcatcgatg tgaccatctt cttcacgttc tggggcatgg atatcatcaa
     2881 tgagaagcgg gtcgatcacc tggagatagc ccccgttgcc aataccagca tgaagatggg
     2941 catgatgggc gtgcccggca acctggggat ccccaatctc atgggcgtgc tgcccggcat
     3001 gacggcgttc tccaccagcc tcatgaagaa gaagatgacc gaggttggag cgccgccggt
     3061 gcgcgaatac ctgcagatgc tcgtggacgg aggcgccaag ctctacgcct gcaagatgtc
     3121 ggcggacatg atggagctga agatggaaga cttcgtcgac ggcgtgatcg acattgtcac
     3181 cgcgagcgac ttcatggacc tgaccgatgg cgcgcagatg gtgttcattt aggagggacc
     3241 ctcgtcggac gacatcctcg gggccggccg ggcgtcacaa gaagcgcgtc ggaagtgcgc
     3301 acgaggtgcc gctatccgcc gggcgcggct ggccccccga tcagcccggt aggcaggtga
     3361 aaccctgctc ggcgaagtgc ccgtcgaggg tgaatgcgtg tcgcgaccct ctcgagcgca
     3421 tcacggcgaa acctaccgcg tccaccaaac tgatgtcgcg acggcgggcg ccacgcacaa
     3481 gcgccagcgc ggtgccatgc acggcctcat ccacccagac gaccttcaac cagggaagaa
     3541 cgaagtcttg gaatgctccc accgcatccg gccccaacct gcgctgcaac aacgcggtga
     3601 gctcaagtag gacgtagttg gtggtgagca gcgacacgtc cgaccgcacg agtcgctccc
     3661 ataccgcgac cacctccgca ctccgcacct gtgcgcggtc gaagacggcg tacagggccg
     3721 aggtgtcgag gaagcagggc tgccccgctc ccggccagga ctcgtcgccc gccgatatgt
     3781 ccctcggccc gctcactgcc ctaggtacgc ctccgccagt tcgtcgtcgt gccgctcggc
     3841 aacgtggggc agaccggaat cgaaggaccc cgcgacacgg agtgcgtcca gacgatcgcg
     3901 gcgggtgagt tcgctcgaag attcgagaac catgtcgacc ccccggcgga tcatctccgc
     3961 gagagagact tcctcggcag ccgcgatctc gcggagccga cgagcctgat cgtcgtgaag
     4021 ctggatttgc gttcgtatca tgaagctacc tccctcgtgg catcatgata acactccatg
     4081 cccacatgct gtatctgaaa gcggcgccgc atcacgagtc tggaaggcac ctacgctcgg
     4141 accgggttcg ccgagcccgc gaccctagcc gaggaacgac gcgcgcacga tctccgggtc
     4201 gaggcggccg acgtgatagg tccgtccgct cctggtgttg gtgatccaca tctcggccgg
     4261 gctgaagaga agcgggtcga gctggtagaa gtccgcgcag gcgcgctcat acaggctgcc
     4321 gaaaggcacg ccccagtccg gggaggccga cggcggcagc atgtcgagaa cggcctcgac
     4381 ggcggcgtct tcacacgtga cagagagcca cgcgcggccg ccgtagatga tcccgtcgtt
     4441 cgtgcggccg acggcgacga ggtcgtcggc ggccaccggc gccacggggc acgagccgaa
     4501 ggcggagatc accgtggtga ggtcgaagcc cgagcggtgc agcttgtata gagccgtctc
     4561 gaccactcga gcggccactt gcaccgatcc cgccaggctc gcggtcggcg ctaccagcag
     4621 gtagaggccc tccgcggaga gacggcagca gcgctctgcg atccagtctg cggcgcggtc
     4681 gccgggcagg tcccacgtct ccatggagag cacggcggct tgcgcgacct ccacatgcgg
     4741 gatgatcgcg aagatgggtt cacgtcggca gagagcgcgc gccggcccag agccgagggc
     4801 gtagaagccg ggggcgtcac cctgcggagg cacttccacg gcccagcccg ccaactgcga
     4861 gcccatgcac gcgagaaggg gatgcctaac gtccacctcg acggccggcg tggaccaacc
     4921 gccgagttcc gccaccttca gccgcacatc tcccagccca cccatgcagg cttcggcgta
     4981 gagacgtccg gcttcgaagg aacccgccgt ccgagcgcca cagtcaatga ccgtggcgcc
     5041 gttgccgagc cggtgagcag caatgcccag ctccgcggaa cgggcgatca tgcgctcaac
     5101 gatagc
//
LOCUS       DASRMJ010000344         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_432305, whole genome shotgun sequence.
ACCESSION   DASRMJ010000344 DASRMJ010000000
VERSION     DASRMJ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432305"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..552)
                     /locus_tag="VFD74_09590"
     CDS             complement(130..552)
                     /locus_tag="VFD74_09590"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK49834.1"
                     /translation="MAQIPENVKELLRGGHNVWVATIGEDGWPNVAIKGSGALLDDDH
                     IYFADIFSKKTRDNLLYNSRVAVGVFAADKNAPDKKVAVQLKGVADMIQTGELFDKVT
                     EQMAALQMGLPPVTYVVRILVDSVWDMSAGPKAGERID"
     gene            complement(536..1552)
                     /locus_tag="VFD74_09595"
     CDS             complement(536..1552)
                     /locus_tag="VFD74_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HZK49835.1"
                     /translation="MAAEIDVYLISGFLGAGKTTFLNRLIKVVPEDLRFLILMNEFGE
                     MGLDGNLVEGEDLDMLEISKGSIFCACVKTDFIKGLHEIAHSVRPDIMLIETTGVANP
                     SDLDRDLKLPIFGGAFNLRDKFCVVDADNFLDTYESFLAIEKQLAASDVFIINKIDLV
                     SPQTVEEIKEIVRGFNPDPRFYETVYADVSFEGLFREGGLRRLAAADDDPDGQGPADS
                     LPVMSEAELEAYIDDLLSDETAEVTPPDRLMSLVVQWPGGSQSELRSWLAGLPERVVR
                     GKGIVRLSGDAWLFSLVNGNWTLEPHKGPAPRDEVVDRMVFISRPEQIKEIAERKIRN
                     GTDS"
     gene            complement(1542..1796)
                     /locus_tag="VFD74_09600"
     CDS             complement(1542..1796)
                     /locus_tag="VFD74_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZK49836.1"
                     /translation="MLVQVLGSGCKKCNDLYDSATTAATRDPVAGHVVEKVADVDEFL
                     RLGVRVTPALVLDGEVVSTGRVLSPDEVADLMTERAGRGR"
     gene            complement(1957..>2643)
                     /locus_tag="VFD74_09605"
     CDS             complement(1957..>2643)
                     /locus_tag="VFD74_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49837.1"
                     /translation="RSAAGVPSPVGLAEEPLGGSATRTEFEAYVPGLIVFSVMMLVFL
                     TAMAVAREVESGTLRRLQLTRMTSFDLMVGVSVAPVLVGVTAVLLTFGTATMVGFHSQ
                     GPLWVAVLVCAVTSLCIVGAGLVVGALVKTVTQAFLAANFPLILFMFFSGAIFPVPRV
                     TLFRLLGHSFGPYDVLPPTHAVAALGKVLTLGEGLGDVAYELSVLSVLSVAYFALGVW
                     LFHRKWLRRA"
BASE COUNT          490 a          943 c          786 g          424 t
ORIGIN      
        1 gtatgcccgc gcccggtaaa cccgcgataa ggtttcgatg agagccttct tacagaccac
       61 ccagcgctgc accctccgcg cggcccatcc ccgtcgatgg gccctgggca cgacatccgg
      121 gagccggcct cagtcgatgc gctcgccggc cttcggcccg gcgctcatat cccacaccga
      181 gtccaccagg atgcggacca cgtaggtgac cggcggcaga cccatctgca gggccgccat
      241 ctgctctgtc accttgtcga acagctcccc agtctggatc atgtcggcca cgcccttcaa
      301 ctgcacggcc accttcttgt caggcgcgtt cttgtcggcc gcgaagacgc ccacggcgac
      361 gcggctgttg tagaggaggt tgtcgcgggt cttcttggag aagatgtcgg cgaagtagat
      421 gtggtcgtcg tcgagcagcg ctccgctgcc cttgatcgcc acgttcggcc agccatcctc
      481 cccgatggtc gccacccaga cgttgtggcc tccccgcaac aactccttca cattctcagg
      541 aatctgtgcc attccggatc ttcctttctg cgatttcctt gatctgctcg ggccgggaga
      601 tgaagaccat acgatcgacc acctcgtcgc gcggcgccgg gcccttgtgc ggttccagtg
      661 tccagttccc attcaccaga ctgaacaacc acgcatcgcc gctcagccgg acgatccctt
      721 tgccgcgcac cacgcgttct gggagcccag ccagccagct gcgcagctcc gactgcgacc
      781 cgcccggcca ctgcaccacc agggacatca ggcggtcggg cggcgtcacc tcggctgtct
      841 cgtccgatag gaggtcgtcg atgtaggctt ccagttccgc ctcggacatg accgggagac
      901 tgtccgccgg cccctgtccg tcggggtcgt cgtcggctgc ggccagcctc cgcaggcctc
      961 cctcgcggaa cagcccttcg aacgagacgt ccgcgtaaac ggtctcgtag aaacgcgggt
     1021 ccgggttgaa cccgcggacg atctccttga tctcctccac cgtctgcggg gacacgaggt
     1081 cgatcttgtt gatgatgaac acgtcggagg cggcgagctg cttctcgatc gccaggaacg
     1141 actcgtacgt gtcgagaaag ttgtcggcat cgaccacgca gaacttgtca cgcaggttga
     1201 aggcgcctcc gaagatgggg agcttgaggt cgcggtcgag gtcactgggg ttcgccacgc
     1261 ccgtggtctc gatcagcatg atgtccggcc gcacggagtg ggcgatctcg tgcagtccct
     1321 taatgaaatc cgtcttgacg caggcgcaga agatcgagcc cttgctgatc tcgagcatgt
     1381 cgagatcctc gccttccacc aggttgccgt ccagccccat ctccccgaac tcgttcatga
     1441 ggataagaaa gcggaggtcc tccgggacga ccttgatcaa acggttcaga aacgtcgtct
     1501 tccccgcgcc caagaacccg gagatgaggt agacgtcgat ctcagcggcc acgaccggcc
     1561 cgctccgtca tcaaatcggc cacctcgtcg ggcgagagca cccgccccgt ggagacgact
     1621 tccccgtcga gcaccagcgc gggcgtgacc cgtaccccca gccgcaggaa ctcgtccacg
     1681 tcggcgacct tctccaccac gtggcccgcg acgggatcgc gcgtggcagc agtcgtggcg
     1741 ctgtcgtaga ggtcgttgca cttcttgcat ccagagccca acacctgcac caacatgtcg
     1801 cacctccaac caggcttcgc tgcaactatt gtagcaaagg ggtccggccg cgcctgcggc
     1861 gagcagcggt agcggcgccg ctaccgctgc tccacggccg cgcggcagcg aacagcgacg
     1921 gccacgcggc aactgatgcc ccggtgggcg cgggactcac gcccgcctta gccacttccg
     1981 atggaacaac cacactccaa gcgcgaagta ggcgaccgag agcaccgaca gcacggacag
     2041 ctcgtaggcc acgtcgccca gtccctcacc cagcgtgagc accttgccga gcgccgccac
     2101 cgcgtgggtg ggcggcagga cgtcgtacgg cccgaacgag tgccccaaca gccggaagag
     2161 cgtcacgcgg gggaccggga agatcgcgcc tgagaagaac atgaacagga tgagaggaaa
     2221 gttcgccgcc aggaaggcct gtgtcaccgt cttcaccagc gcacctacca ccaacccggc
     2281 gccgacgatg cagaggctcg tgaccgcgca caccagcacg gccacccata gagggccctg
     2341 gctgtggaag cccaccatcg tcgcggtgcc gaacgtcagc agcacagccg tcacccccac
     2401 gagcaccggc gccacactca caccgaccat gagatcgaac gacgtcatcc gcgtgagttg
     2461 aagacggcgc agcgtcccgg actcgacctc tcgggccacg gccatcgccg tcaagaacac
     2521 cagcatcatc acagagaaca cgatgagccc gggcacgtac gcctcgaact cggttcgggt
     2581 cgcggaccca cccaggggct cctcggccag accgacggga ctcggcacgc ccgcggcact
     2641 ccg
//
LOCUS       DASRMJ010000345         4382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_357509, whole genome shotgun sequence.
ACCESSION   DASRMJ010000345 DASRMJ010000000
VERSION     DASRMJ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357509"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFD74_09610"
     CDS             complement(<1..257)
                     /locus_tag="VFD74_09610"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HZK49838.1"
                     /translation="MQGTPQDTRLEGSIKGSSAHPDGLQAAYRLDPDYVDSWLRSRLE
                     WMDGGVAQRVRIVARLYSGERVSGQDLAHDLNISRAAVHKHV"
     gene            301..2121
                     /locus_tag="VFD74_09615"
     CDS             301..2121
                     /locus_tag="VFD74_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZK49839.1"
                     /translation="MTVGILERVWLGGWKARLVMAVAVVVALVVFAILVDTALSYNRV
                     HSGISISGVDTGRMSPEYAEADLTRRVERSANNPVTLVADDGRTWQVLPADVGVQVDE
                     AATVQAAMAVSRDGNVLQDLKSRFSLYFSKKDVPLDATLDPALLDTLLEGVASALDVS
                     AINAGLAIDSNDISVIEGREGLVVDRGAVRTEVKKLLFSLTSGRVTIPMKVQTPTIQA
                     ADTSGAVDMAKTMLSGAVGLEYGDVVWALSPAEIARAIDFTTEGEGADSRLIPFLSAD
                     RLAGFFEDVAKAVKKDPANATWTTDGEKATLVPGVPSTSLDSVKTAEDLTRAALSPDA
                     RVAKVQLLVTPAGRTTEMAEQMGIVAKLAAFTTEFGGSEGRRKNVMHAAELISGTLLA
                     PGQEFDFDTVVGQRTAANGFTTAPAIIAGKLEETLGGGICQVSTTLFNAVFFAGLDVT
                     ARSNHSLYISHYPMGRDATVSWGGPAFRFVNDTPKWILIKAAASRSSLTFVIYGRPQN
                     RQVTHTTGDWYGIQPPTEKRVKTGELFEGEQRVADEGQTGRSVQVTRLVTQDGKTIHQ
                     DTFTSKYPMRPKIIEEGAKPTTTTTLPTTSTTATTTTTAP"
     gene            2347..2952
                     /locus_tag="VFD74_09620"
     CDS             2347..2952
                     /locus_tag="VFD74_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK49840.1"
                     /translation="MTLPFALAVEDIPARDITEEDVKLFAVRASNDDPAAFGMLYRLY
                     FPQLFTFINFRVSSREDAEDLANAVFEKALRAIERYQPKPAQFSTWLYTIAKNCIIDH
                     YRRRRLPVDSEADAAVIPSTDLANDPMPRVLSEERKRTLREALLDLTPDQREVVECRF
                     FFELSIQETAAAMLKTEGAVKALQFRALEGLQKLLTEDIIL"
     gene            complement(2942..3493)
                     /locus_tag="VFD74_09625"
     CDS             complement(2942..3493)
                     /locus_tag="VFD74_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49841.1"
                     /translation="MQSRTMGIVLMIVGVGIAVWLGAGLATGKSVTGAALGFVLFSLP
                     FFAVGAYFLMRSGGEIVQQSKADKERAILNSVMTRGRVGVADLAIENDCSRDEVRDYV
                     YDLIGKGLFTGYVNWDKGELVSAEAAQIKEGQCPNCGGAVELAGKGLVRCPFCGTETY
                     LSGPAPGAAADAADAAGASTPTG"
     gene            complement(3642..>4382)
                     /locus_tag="VFD74_09630"
     CDS             complement(3642..>4382)
                     /locus_tag="VFD74_09630"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49842.1"
                     /translation="LEDKFYEHVDPICKDVYKDVTSLGLFTGDPARTRTKWKILGFLV
                     LSGFVAFSGLLAVSDVPGWGWLLVGGVVSGVIVLGFARSMPQRTSLGAQEQRKWEAFR
                     NYLQDLTRYQDMATARETFDRYLPYAIAFGVERDWVRRFEQLELPAPIWYRPMWVPGT
                     GTGAGPVVADVGGMPGGMPAGVPGGLGGLSLDNISDGLFSSLNSMSNVLTSKPSSSGS
                     GSGAWGGGGGGFGGGFSGGGGGGGFRAG"
BASE COUNT          791 a         1553 c         1382 g          656 t
ORIGIN      
        1 acgtgcttgt gcaccgcagc gcgggagatg ttgaggtcgt gggcgagatc ctggcccgag
       61 accctttcgc ccgagtagag tcgcgcgact atgcgaacgc gctgcgccac cccgccgtcc
      121 atccactcca gacggcttcg cagccaagaa tccacgtagt ccgggtccag acggtacgcc
      181 gcctgaaggc cgtccggatg ggccgatgaa cctttgatgc ttccctcgag tctggtatct
      241 tgtggcgtcc cctgcatcca gcaggacccc tcgattcctg gaaaggtgtg tggcggttcg
      301 atgaccgttg ggatactcga acgcgtatgg ctgggcggtt ggaaggctcg gctcgtcatg
      361 gctgtggccg tcgtcgtcgc cctggtggtg ttcgcgattc tagtggacac cgctctctcc
      421 tacaatcgcg tgcactccgg gatatccatc tccggagtag acacgggtcg catgtccccg
      481 gagtacgccg aggccgacct cacccgacgg gtagagcgct ccgccaacaa cccggtcacg
      541 ctcgtcgcgg acgacggacg cacgtggcag gtactgcccg ccgatgtggg cgtgcaggtt
      601 gacgaggccg ccaccgtcca agcggctatg gcggtctcgc gcgacggcaa cgtcctgcag
      661 gacctcaaga gccgcttctc gctctacttc tcgaagaagg acgtgccgct ggacgcgacc
      721 ctcgacccgg cgctcctcga cacgctgctc gagggggtcg cgagcgccct cgacgtctcc
      781 gcgatcaacg ccggcctggc catcgacagc aacgacatct cggtgataga ggggcgtgag
      841 ggcctggtcg tcgacagggg cgccgtgcgg accgaggtca agaagctgct gttctcgctt
      901 acctccggcc gagtcaccat cccaatgaag gtccagaccc cgaccataca ggccgccgac
      961 accagcgggg ccgttgacat ggccaagacc atgctgagtg gagcggtggg cctggagtac
     1021 ggggatgtcg tgtgggctct gtctcccgcg gagatcgccc gggctataga ctttacgacc
     1081 gagggcgaag gagcggattc tcgcctgata cccttcttgt ccgccgacag gctggccggc
     1141 tttttcgagg atgtcgccaa ggccgtcaag aaggaccccg cgaacgccac ctggacgacc
     1201 gacggcgaga aggccacgct cgtccccggc gtcccgagca cgagcctcga ctcggtcaag
     1261 accgcggaag acctcacgcg ggccgccctc tcacccgacg cgcgggtcgc taaggtgcag
     1321 ctgctggtga ctccggcggg acgcaccacc gagatggccg agcagatggg catcgtggcc
     1381 aagttggccg ccttcacgac ggagttcggc gggtcggagg gtcggcgcaa gaacgtcatg
     1441 cacgcagccg agctcatcag cggcacgctc ctggccccag gccaagagtt cgatttcgac
     1501 acggtggtgg ggcagcgcac ggcagccaac ggattcacca cggcaccggc gatcatcgcc
     1561 ggcaaactgg aagagacgct tggcggcggc atttgtcagg tctccaccac gctcttcaac
     1621 gccgtcttct tcgcgggact cgacgtcacc gccaggtcga accactccct ctacatcagt
     1681 cactacccca tgggccgcga cgcgaccgtc agctggggag gtccggcgtt caggttcgtc
     1741 aacgacacgc ccaagtggat cctgatcaag gcggcggcct ccagaagcag tctcaccttc
     1801 gtgatatacg gcaggccgca gaaccgccaa gtgacccaca cgaccggaga ttggtacggc
     1861 atccaacctc ccaccgagaa gcgtgtgaag accggcgagc tgttcgaggg cgaacaacgc
     1921 gtggccgatg aaggccagac gggccggagc gttcaggtga cccgcctcgt gacccaagac
     1981 ggcaagacca tccaccagga taccttcacc agcaagtacc cgatgcggcc gaagatcatc
     2041 gaggagggcg ccaagcccac cacgacgacg accctgccca ccaccagcac cacggctacc
     2101 accacgacga cggccccgta ggaagcagcg gagcccccgg ccgggggctc catgtccccg
     2161 gcaaaggccg gcagcggcac gcgggccccg gcagcccccc acgcctcagg cgcttccgcg
     2221 cctcgccgcg gcggcgacca agcggcgccg gcacgccggc cccgccggcg tgccgggact
     2281 caagcgaacg gcatccgccg ccgatacccg tgccatggac caggcaacga caaggcggct
     2341 gccgacatga cgctcccgtt cgccctagcc gtcgaggaca tcccggctcg ggacatcacc
     2401 gaggaagacg tcaagctctt cgccgtgcgc gcgtccaacg acgacccggc cgccttcggc
     2461 atgctctacc gcctctactt cccgcagctg ttcaccttca tcaacttcag ggtgtcgagc
     2521 cgagaagacg ccgaggatct ggcgaacgcg gtgttcgaga aggctttacg cgccatcgag
     2581 aggtatcagc ccaagcctgc gcagttctcc acgtggctct acacgatcgc caagaactgc
     2641 atcatcgatc actaccgccg gcggcgactg ccggtcgatt ccgaagccga cgcggcggtc
     2701 atcccctcga ccgatctcgc gaacgacccc atgcctcggg tgctctcaga agagcgcaag
     2761 cggacgctgc gcgaggccct gctggatctg acgcccgacc agcgcgaggt cgtggagtgc
     2821 cgcttcttct tcgagctttc gatccaggag accgcggcgg cgatgctgaa gaccgagggc
     2881 gccgtcaaag ccctgcagtt ccgtgcgctc gaaggtcttc agaagcttct caccgaggac
     2941 atcatcctgt aggagtcgaa gcgccggccg cgtcggcggc gtcggccgcg gccccgggag
     3001 caggccccga caaataggtc tccgtgccgc agaacgggca ccgcaccaag cctttgccgg
     3061 ccaactcgac ggctcctcca cagttcgggc actgcccttc cttgatctga gcggcctccg
     3121 ccgagacgag ctcccccttg tcccagttga cgtagccggt gaagagaccc ttcccgatga
     3181 ggtcatacac gtagtcgcgc acctcgtcgc gcgaacagtc gttctcgatg gccaggtcgg
     3241 ccacccccac gcggccgcgg gtcatcacgg agttgagtat ggcgcgttcc ttgtcggcct
     3301 tcgactgctg cacgatctcg cccccggacc gcatcaagaa atacgcgccc acggcgaaga
     3361 aggggagaga gaagaggacg aacccaaggg ccgccccggt caccgacttg ccggtggcca
     3421 acccggctcc cagccaaacg gcgatcccca ccccaacgat catcaggacg atgcccatgg
     3481 tcctgctctg catcacgcct ccttgctgtt cgtgcggcgc cgcccgagcc gggcggcgcc
     3541 gcgcaacctc ttcggtcaca ctctctctgc ggcgccgccc caagccgcga ccgcgccgga
     3601 cgggcgcggc gcggcgtccg cgggcgggcc gagcgcctcg cctatccggc ccggaagccg
     3661 cctccgccgc cgcctccgga gaacccgccg ccgaaacctc ctccaccgcc gccccaggcc
     3721 ccgctgccgg aaccggagct ggagggtttc gaggtgagca cgttcgacat gctgttcaga
     3781 ctgctgaaga gcccgtcgga gatattgtcg agactcaggc cgccgagacc gcccggcaca
     3841 cccgcaggca tgccgcccgg catgccgccg acgtcggcga ctacaggccc ggcgcccgtt
     3901 ccggtgccgg gcacccacat gggccggtac cagatgggag ccggcaactc cagctgttcg
     3961 aacctccgca cccagtcgcg ctcgacgccg aaggcgatgg cgtacgggag gtagcggtcg
     4021 aaagtctcgc gggccgtcgc catgtcctga tagcgggtga ggtcctgcag gtagttgcgg
     4081 aacgcctccc acttgcgttg ctcctgcgca ccgagcgagg tccgttgcgg catcgagcgg
     4141 gcgaacccga gcacgatgac gcccgagacg acgccgccga cgagaagcca cccccacccc
     4201 ggcacgtccg acaccgcgag caaccccgag aacgccacga agcctgacaa cacgaggaag
     4261 ccgaggatct tccacttggt gcgggtgcgg gcggggtcgc ccgtgaacag cccgagtgag
     4321 gtgacatcct tgtagacatc cttgcagatg ggatccacgt gttcgtagaa cttgtcctct
     4381 ag
//
LOCUS       DASRMJ010000346         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_319423, whole genome shotgun sequence.
ACCESSION   DASRMJ010000346 DASRMJ010000000
VERSION     DASRMJ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319423"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..850)
                     /locus_tag="VFD74_09635"
     CDS             complement(152..850)
                     /locus_tag="VFD74_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK49843.1"
                     /translation="MALIKVDSVHKEYGATDARVVALHEVSLEIRAGEYAAIMGPSGS
                     GKTTLLSILGAMNPPTSGSLSIDGIDVYSLSQERQADLRREYIGFVFQQLELVPYLTA
                     LENVMLPLAILKEKGKREKALEALARVGLDERKSRRLPSELSGGEQGRVAIARAVVND
                     PPIVLADEPVGSLDTATGRQILEQLRTLANSGHAVIMVTHNPESTRDVDRVIEILDGR
                     VTCDDGPSVCTVAV"
     gene            complement(868..2025)
                     /locus_tag="VFD74_09640"
     CDS             complement(868..2025)
                     /locus_tag="VFD74_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49844.1"
                     /translation="MELRHLAFGYLHRRKGKAALITFGLSVAVGAIVLVLSLVLSLRA
                     TMDERLTQYGSNLIIMPASAELNLSYGGMSVGSAGNGEIPRLSESDVEAVRAVPSASA
                     MSAVLPVLLAPVDVQDRTLLGLGTDLPTSLRFKPWWKLEGSVPAGGGEVLVGLNVRNE
                     LGLELGDEVVISGRSFRVSGILWETGGEEDNLVFFDRPVLADLTGQAGVVNLIEVTAA
                     NTDVIDQLTAEIQNVLPEASVQSVKSSIEFTEQANSALADFGLAVTLLIVVIAGAVVM
                     VTMLTAVKERQKEIGVLRAVGYKRRHIASLILLESAMLSSAGAVVGTIGGLAGALVVP
                     KLVGGLTLGLVVHPLVIVAGLGVAYVVGLSAALYPAWRAAELDPVTALKYI"
     gene            complement(2028..>2673)
                     /locus_tag="VFD74_09645"
     CDS             complement(2028..>2673)
                     /locus_tag="VFD74_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZK49845.1"
                     /translation="GETLRVTGVLDPTGTQEDELVYIDLGAAQRLWNRPDELSFIEVA
                     AWCSTCPIETINAQISAEIPNARVSAVLKAAESREILIGQFRLFSWVLSGFMTLVGCL
                     IVITITLGSVRDRRGEIGIFRSVGYRRRHIFKIILIENLALALLGGVLGVALAVAAHG
                     PVARMVAGVATTSVPGPLDLGAALAATFFVVLLASLYPARRAAGLSPTLAMRRV"
BASE COUNT          500 a          901 c          842 g          430 t
ORIGIN      
        1 gtagcagatt gccttctata cccccacagg tatattaggc gacaccttgc ctcgcgggcg
       61 ccgccgacgg tgcgcagcac aggtgcggcg cacgcgaatg ccgcgcgggt gggccacgaa
      121 ggtgcccccc gctccgacgg gcgactgagg gctagaccgc caccgtgcac acggagggac
      181 cgtcgtcaca ggtgacgcga ccgtcgagga tctcgatcac cctgtccacg tcgcgggtgg
      241 attcggggtt gtgcgtgacc atgatcaccg cgtggccgct gttggcaagg gtgcggagtt
      301 gctcgaggat ctgtcggccc gtggccgtat ccaggctccc caccggctcg tcggccagca
      361 cgatgggagg gtcgttcacg accgcgcggg ctatggccac gcgaccctgc tcgccacccg
      421 agagctccga ggggagacgg cgactcttgc gctcgtcgag acccacgcgg gccagcgcct
      481 ccagcgcctt ctcccgcttg cccttctcct tgaggatggc caggggcaac atgacgttct
      541 caagagcggt caagtacggc accaactcca gctgctggaa cacgaagccg atgtactccc
      601 gccgcagatc ggcttgacgt tcttgcgaaa gggagtagac atcgatgccg tcgatcgaga
      661 gcgagccgga ggtgggtggg ttcatcgctc cgagaatgga cagaagggtg gtcttgcccg
      721 acccggacgg acccatgatg gccgcatact ccccggctcg tatctcgaga ctcacctcgt
      781 gcagggccac gacgcgggcg tcggtggccc cgtattcctt gtgcacgctg tctaccttga
      841 tcaaagccac aggtttcctc cagcgattca aatgtacttg agggccgtca cggggtcgag
      901 ttcggcggcc ctccaagcgg gatagagcgc ggcactcagc cccaccacat aggcgacgcc
      961 caggccggcc acgatcacca atggatggac taccagaccg agggtgaggc cccccaccag
     1021 tttcggcacc accaaagccc cggcgagccc accgatggtc cccacgacgg cccccgcgga
     1081 gctgagcatc gccgattcga gcagtatgag cgaggcgata tgcctgcgct tgtagcccac
     1141 cgcccgcaac acgccgatct ccttctgcct ctccttgacg gcggtgagca tggtcaccat
     1201 caccaccgcg ccggcgatga ccacgatcag aagagtgacc gccagaccga agtcggccag
     1261 agcggagttg gcctgctcgg tgaactcgat gctgctcttg accgactgca ccgaggcttc
     1321 cgggagcaca ttctgtatct cggcggtgag ctgatcgatg acatcggtgt tggcggcggt
     1381 cacctcgatc aagttcacga cgcccgcctg cccggtgagg tcggccagca ccggacggtc
     1441 gaagaacacc agattgtcct cttcgccgcc ggtctcccat aagatccccg agaccctgaa
     1501 gcttcgcccg gagatgacca cttcgtcgcc cagctcgaga ccgagctcgt tgcgcacgtt
     1561 caatcccacg agcacctcgc caccacccgc ggggacactg ccttcgagct tccaccacgg
     1621 cttgaagcgt agactcgtcg gtagatcggt gccgagaccg agaagggtgc gatcctgcac
     1681 atcgacgggc gccaagagga ccggaaggac tgcactcatc gcggaagccg agggcaccgc
     1741 gcgcacggcc tcgacatcac tctcgctgag ccgtgggatc tccccgttgc ccgccgaccc
     1801 gacgctcatt cctccgtagg ataggttcaa ctcggcggaa gcaggcatga tgatgaggtt
     1861 ggatccgtac tgggtgaggc gttcgtccat cgtggcgcgc aaggagagca ccagcgagag
     1921 caccaggacg atcgcaccga ccgcgaccga gaggccgaag gtgatcaagg ccgccttccc
     1981 cttgcgccgg tgcaggtatc cgaaagccag atgtcgcaac tccacggtca gaccctccgc
     2041 atggccagcg tcggactcag gccggccgcc cggcgcgccg ggtagaggct cgccagcagt
     2101 accacgaaga acgtcgcagc aagagccgcg cctagatcga ggggtccggg gaccgatgtc
     2161 gtggccactc cggccaccat gcgggccacc ggtccgtgtg ccgcgacggc tagagccacc
     2221 ccgagcaccc ccccgagcag agcgagggca agattctcga tcaggattat cttgaagatg
     2281 tgccggcgac ggtaacccac ggagcgaaag atcccgatct cgccgcggcg gtcccgcacg
     2341 ctgccgagcg tgatggtgat gacgatgagg cagccgacca gcgtcatgaa cccggagagg
     2401 acccagctga agaggcggaa ctgcccgatg aggatctcac gcgactcggc cgccttcagg
     2461 accgccgaga cacgcgcatt cggtatctcg gccgatatct gggcgttgat ggtctcgatc
     2521 ggacacgtgc tgcaccacgc ggcgacctcg atgaaactga gttcgtccgg cctgttccac
     2581 agtctttgtg ccgcgcccag atcgatgtat acgagttcgt cctcctgcgt tccggttgga
     2641 tcgaggacgc ccgtcacccg aagggtctcg ccc
//
LOCUS       DASRMJ010000347         3660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_212768, whole genome shotgun sequence.
ACCESSION   DASRMJ010000347 DASRMJ010000000
VERSION     DASRMJ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212768"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(931..1611)
                     /locus_tag="VFD74_09650"
     CDS             complement(931..1611)
                     /locus_tag="VFD74_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49846.1"
                     /translation="MVANDSVRIAEEGQRLTGGEDLGSHTVRVTNRHQDQLGGSVFRV
                     AQPLQPEMTLPDIDELKAGIGRYGRHRGRRPRRSPTGSTSASYATWAMDDDVRPGRDP
                     ERRSGSDRRARLRTLTAGSPRLLAHIDDSRCLGCAGCLGSCQKEALRIERRPELPHVP
                     DDVAEFLVRSMIERGRVADLLIDGTNGRGRAFANAALGALLSLRPAERLLASEGLRSR
                     FVKSAQKS"
     gene            1674..2102
                     /locus_tag="VFD74_09655"
     CDS             1674..2102
                     /locus_tag="VFD74_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK49847.1"
                     /translation="MDKTDAARVVELLEDVRRLFKEHVSGGGASPGLPLTTIVILREI
                     GRQPGVSVSALGRRTGLSKGHVSNVVDRMSIDGLLEKRPDPEDQRCVRIDLTDEGAGL
                     LSRLDHRYQGFWAELLGDVDDIVAARIIDGLESLRCSLRP"
     gene            2084..3658
                     /locus_tag="VFD74_09660"
     CDS             2084..3658
                     /locus_tag="VFD74_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK49848.1"
                     /translation="MLSAPMNAMRSTQPKTVDPSRSSLGEDTGAHPNPWLVLLSVALG
                     MFMVVVDVSILNIALPQIAGSLHAGMSSIQWTLIAYTLLMTTLVPLFGRISDVIGRKR
                     LFILGMAIFTLGSLLAALSPSIHWLIGARLVQAVGGALITTNTLAIIADVFPPGKRGV
                     AMGVQSILVSGGAAIGPTLGGFLVTHFGWEAIFYVNLPVGLIAVTLALKILPSLRSHR
                     AREPLDWPGAGLLMTSLASILLAMTQGPSWGWTSSAILGLAVVGVAAASVFVWWELRA
                     RYPLVDLTLFRNRAFSAGQLAGLSGTVAFASMMFLLPFYWQGLRGLSAQQAGIMMLPL
                     PMVLMVVAPISGRLSDGYGARAIASTGLTVVAVALLLISRIGAETPVWSVLVRVAVMG
                     LGLGMFMAPNNNAVMSSVGAHRRGIASGLLATFRFTGQSVGIAFVGAVVGSLMRSGTG
                     VVGNALPSPDRFRAVAGDPIALAALSDTFVHAMHVASLAAIPFALLGVFFSLWRPGSV
                     GSGGPHTGRGQDAEEI"
BASE COUNT          608 a         1185 c         1209 g          658 t
ORIGIN      
        1 gagcagcagc aggagaacga cagcgcgagc cggaaagccg agccacgcgc ttcgacgctc
       61 ggctgtgatt ggtgagcaaa gcatgggggc atcctagcac gccgcccgga tgcccgggca
      121 aggcacgcga tgcgacactg aggtcgaaac atgcaaatga cagcttccgt ccgacccccc
      181 tgctacagtc atcatgcctg cgacgtgtcg ttccgtcata accgacgaga agggtgtgct
      241 agctccctcg gtaaagaggg gtcccgggct gtcgaacgga gcggcgacag accaagaccg
      301 gaccatcacc attgttccat ggtggagggc gagtgggttg accgcaatgc catccgcatc
      361 cgtctcagat tgttggttgc cggacttcaa cggagccgcc tgaggcagaa aagcgccccg
      421 ccccgggggg tacgtgggga cggggcgggt gccgccgaag cagcattcgg atagggcaaa
      481 caaggagccc ccggaagcgt ctgatcctgg ctaaaaagga cgcgtgtcgg aggctgattc
      541 tcacctcggg atccacatgc ggtcaacgcc gcggatccta gctctatgta aggacctacc
      601 tctgggggta gctctgtaaa cgtacggaag catgccgctc aggtgaatca ccgtccaggc
      661 atcgggagga caagggcctg ctcccgctcg acaccctccc actggccgcg gccagtggga
      721 ggaagggcaa gaagcgcgcg cccattctct caccaggaat ccacaggcca ttctggccgg
      781 gcgcaaggac tgctacggga gcgccgcact tgcgaacagc gcggccggcc cagaaccagt
      841 cgccgccggg gggcaggcga ccagctgggt ggtagccgcc ctcccaccgt ccggcacgat
      901 cacttcatcg tgcgcaggtg aacgaagcgc tcagctcttc tgggcggact tcacgaagcg
      961 ggagcgcagg ccctcgctcg cgagaaggcg ctcggccggc cgaagcgaca gtagtgcacc
     1021 gagcgccgcg ttcgcaaagg cccgtccacg cccgttcgta ccgtcgatga gaaggtcggc
     1081 cacccgaccg cgctcgatca tcgagcgaac aaggaactcc gccacgtcat cagggacgtg
     1141 gggcagctcc gggcggcgct cgatgcggag ggcctccttc tggcacgaac cgaggcaccc
     1201 cgcgcacccc aggcagcgac tgtcgtcgat gtgtgcgagg agacgcggag acccggccgt
     1261 cagagttcga agccgcgcgc ggcggtctga gccgcttctc cgttcaggat ctcggccggg
     1321 tcgaacgtca tcgtccatcg cccaggtcgc gtacgaagcc gaagtgctgc ccgtcggcga
     1381 gcggcgcggt cggcggccgc ggtggcgacc gtatcgaccg atcccggctt tcaactcatc
     1441 gatgtcgggg agggtcatct caggctgcag gggctgggcc actcggaaga cggaaccgcc
     1501 aagttggtcc tgatgacggt tcgtgacgcg cacggtgtga gatccgagat cttcgccgcc
     1561 ggtgagacgc tggccctctt cggctattct gaccgaatcg ttcgctacca tacgatttgt
     1621 gctagcctaa tcgttctcgc acatcctcgg atcggcgtcg gagcacaaca cagatggaca
     1681 agaccgatgc cgcgcgcgtc gtcgagctac tcgaagacgt acgccggctc ttcaaagagc
     1741 acgtcagcgg aggaggagct tctccgggac taccgctcac cactatcgtc attctgcgcg
     1801 agatcgggcg gcagcccggc gtaagcgtga gtgccctggg gcggaggacg gggctttcga
     1861 aaggccacgt ctccaacgtc gtggaccgca tgagtatcga tggattgctc gagaaacgac
     1921 ccgacccgga agatcaacgc tgcgtgcgga tcgatctgac cgacgagggc gctggacttc
     1981 tctctagact cgatcacagg taccagggat tctgggcgga gttgctgggc gacgtcgacg
     2041 acatcgtcgc ggcccggatc atcgacgggc tggagagcct gcgatgctct ctgcgcccat
     2101 gaacgccatg cgatcaacac agccgaagac tgttgaccca tcccgttcct ctctcggcga
     2161 ggatacgggg gctcacccca atccctggct ggtgcttctg agcgtggcgc tcgggatgtt
     2221 tatggtcgtc gtcgacgtgt ccatcctgaa catcgccctt ccgcagatcg cggggagcct
     2281 gcacgccggc atgagcagca tccaatggac gctcatcgcc tacacgctcc tgatgaccac
     2341 tctcgtgcca cttttcgggc ggatcagcga cgtcatcggg cgcaaacgac tcttcatctt
     2401 ggggatggcc atcttcactc tggggagtct gctggccgcc ctctcgccct ccatccactg
     2461 gctgatcggg gctcggcttg tccaggcggt cggtggggct ctcatcacga cgaacacgct
     2521 ggccatcatc gccgacgtct ttccgccggg aaaacggggc gtggcgatgg gggtgcagtc
     2581 catcctggtg agcggggggg ctgctatcgg accgacgctc ggcggctttc tggtgaccca
     2641 cttcggctgg gaggccatct tctacgtgaa cctgcctgtg ggcttgatcg cggtcaccct
     2701 cgccctcaag atcctgcctt ccttgcgcag ccatcgtgcc cgagaacccc tcgattggcc
     2761 gggcgcgggg ctgttgatga cgagcctcgc ctccatcctg ctggccatga cgcagggccc
     2821 ctcctggggc tggacgtcga gtgccatact cggcctggcc gtcgtgggcg tagccgccgc
     2881 ctcggtcttc gtctggtggg aactgcgcgc gcggtatccg ctcgtggatc tcacgctctt
     2941 ccgaaacagg gcgttcagtg ctgggcagct ggccgggttg tctgggaccg tcgccttcgc
     3001 ctcgatgatg ttcctgctcc ccttctactg gcaggggctg cgcggcttga gcgcgcagca
     3061 ggcgggcatc atgatgctcc cgctccctat ggtactcatg gtcgtggcac cgatctcggg
     3121 gcggctctcc gacggctacg gcgcccgggc catagcctcg accggactga cggtcgttgc
     3181 cgtcgccctg ctcctcatct cgcggatcgg cgctgagacg ccggtctgga gtgtgctcgt
     3241 gcgcgtggcg gttatgggct tggggctggg catgttcatg gcccccaaca acaacgccgt
     3301 gatgtccagt gtgggggccc accggcgcgg catcgcctcc ggtctcctag cgacctttcg
     3361 cttcaccggt cagagtgtgg gcatcgcctt cgtgggggcc gtggtgggtt ctcttatgcg
     3421 ttccgggacc ggggtagtgg gcaatgccct cccctcgccc gacaggttcc gggcggtggc
     3481 cggggatccg atcgctcttg ccgcgctgag cgacaccttc gtgcatgcca tgcacgtcgc
     3541 aagcctcgcc gccatcccct tcgccctcct gggggtcttc ttctccctct ggaggccggg
     3601 ctcggttggg agcggagggc cccataccgg gagagggcag gatgccgagg agatctgaac
//
LOCUS       DASRMJ010000348         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_213035, whole genome shotgun sequence.
ACCESSION   DASRMJ010000348 DASRMJ010000000
VERSION     DASRMJ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213035"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..909
                     /locus_tag="VFD74_09665"
     CDS             226..909
                     /locus_tag="VFD74_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HZK49849.1"
                     /translation="MTGLGTVLNMGAVIAGTAVGVGVGQRLPARVREIVTDGLGLLTI
                     LMGLRMALETEQFLVVLGSVLVGGLIGEALDIEHRLERVGDRLQARLSTGSSTFSTGF
                     VTASIMFCVGPMAVLGSIEDGLRGTVEILSVKSILDGFASLAFASTLGWGVGFSALSL
                     LVYQGALTLGAGFFERVLTDSMIAEMTAVGGLLVLSMGLRLLQVREIRTGNFLPALVL
                     APVIVALAG"
     gene            1060..1386
                     /locus_tag="VFD74_09670"
     CDS             1060..1386
                     /locus_tag="VFD74_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49850.1"
                     /translation="MGFFDKLTVGAEKVAKQAEKALEQGKTKVEELQLERQMDGLAKK
                     LGYMEYDVSRGREVDAAIRQSYLDEIAGLEEQIALNKAQREAAAVVEEPAAAAVTPDA
                     PPEATE"
     gene            1530..>2084
                     /locus_tag="VFD74_09675"
     CDS             1530..>2084
                     /locus_tag="VFD74_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013708507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49851.1"
                     /translation="MVRCSRAGRSTRVERQVGAHSAEHAEHAEHARGIRGAQSVDRAV
                     DACGEPTMMPALFDLRYEGGIIQEMGSLEPRPGERVIDLDGAYLCAGLVDAHVHLALG
                     GASVTAPPPPTARRVAASPESAEDLTREAVVAANGANHLAHGVTAVRDLGGYTSVAPA
                     ARGGTRPLVVSSAGALTRRGHYGAF"
BASE COUNT          344 a          641 c          757 g          342 t
ORIGIN      
        1 gatgtggcct atgacgtcat acggaggcgc ggtcacagag tagaggcttc cggcgcgccc
       61 gaggtcgtat ctccacgcac ggaaagggcc aaccgttgcc actgcatctc ctcgggaatc
      121 gctcgagaac cggctccgtt gctcttgctg cggggggcgg ggcgcggcac agaatagcga
      181 atgatatcat ccgcagcctg ctgaggtccg agtgtggagg tgggtgtgac cgggttggga
      241 accgtcctca atatgggtgc cgtcatcgcc ggcacggcgg tgggcgtggg cgtagggcag
      301 cgacttccgg ctcgggtgcg cgagatcgtt actgatggcc tcggcctgct cacgatcctc
      361 atggggttgc gcatggcgct cgagacggag cagttcctgg tggtactcgg cagcgtcctg
      421 gtgggaggac tgatcggcga agccctcgat atcgagcatc gcctcgaacg ggtgggtgat
      481 cgacttcaag cgcgactctc caccgggtcg agcacgttca gcaccgggtt cgtcaccgcg
      541 agcatcatgt tctgcgtggg gcccatggcg gtgctcgggt cgatcgaaga cgggctcaga
      601 gggacggtgg agatcctgtc ggtcaagtcg atcctcgacg ggttcgcctc gctcgccttc
      661 gcttcgacgc tcggttgggg ggtgggcttc tccgctctgt cgctgctcgt gtatcaggga
      721 gcgctcacgt tgggagccgg gttcttcgag cgtgtcctca ccgattccat gatcgccgag
      781 atgaccgcgg tgggtggtct gctcgtgttg tccatgggac tgcgcctgct gcaggtgcga
      841 gagatccgca cggggaactt cctcccggct ctggtattgg ccccggtgat cgtcgcgctc
      901 gccgggtgaa ggctgcggct tgagcacgcc gctccgccgc cgacccgacc tcgtcacgcc
      961 acggacggcc accgaagagg cttcggacat gtgcaggaga ggcccaggat gggaacggtc
     1021 cgcctgtggg acagaaatca gaccggagga gatgcagaca tgggattctt cgacaagctg
     1081 actgtaggcg ccgaaaaggt ggcgaagcag gctgagaaag ccctcgagca aggcaagacc
     1141 aaggtcgagg aactccagct cgaacgccag atggacggtc tggcgaagaa gctcggatac
     1201 atggagtacg atgtgtcgcg cggccgcgaa gtggatgccg cgatccggca gtcgtatctc
     1261 gatgagatcg cggggttgga ggagcagatc gctctgaaca aggcgcagcg cgaggcggcc
     1321 gccgtcgtcg aagaacccgc agccgcggcc gtgacccccg acgcaccgcc ggaagcgacg
     1381 gaatagcccg agggcggagg ccgtagcgcg gacggatgcg gaagctgaag agcgcgggcg
     1441 acacgacgcg cgagccgggt ggacggagct cggctgggac gagcgttgag ccccactcgg
     1501 ttctcgatct gagcggagca tcccacctgt tggtccggtg ctctcgtgcc ggacggtcca
     1561 ctcgggtcga gcgccaggtc ggcgcccaca gcgccgaaca cgccgaacac gccgaacacg
     1621 cccgggggat ccgcggcgct cagagtgtcg accgagccgt cgatgcctgc ggcgaaccca
     1681 ccatgatgcc cgcccttttc gacctgcggt acgaaggcgg gatcatccaa gaaatggggt
     1741 cgctagagcc ccggcccggc gaacgtgtaa tcgatctgga cggagcctac ctgtgcgccg
     1801 gcctcgtgga tgcgcacgtg catctggctc tgggcggcgc gtccgtgacg gcccccccgc
     1861 cgccgaccgc gcgccgagtc gcggcctcgc cggagagtgc cgaggatctc acgcgtgagg
     1921 ctgttgtggc cgcgaacggc gcgaaccacc ttgcgcacgg tgtgactgcc gtccgcgatc
     1981 tgggggggta cacgagtgtc gcaccggcgg cccgcggcgg gacgcggccg ctcgtggtga
     2041 gttccgccgg agccctcacg cgtcgcgggc actacggcgc gttc
//
LOCUS       DASRMJ010000349         8722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_283087, whole genome shotgun sequence.
ACCESSION   DASRMJ010000349 DASRMJ010000000
VERSION     DASRMJ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283087"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="zwf"
                     /locus_tag="VFD74_09680"
     CDS             complement(<1..1002)
                     /gene="zwf"
                     /locus_tag="VFD74_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZK49852.1"
                     /translation="MILGVTGDLSNRKLMPSLYRLYVRGLLPPGIAIVGVGRRAWDRL
                     EFQQRVRGALDEHLGAPVQEEMWRGFAETLHFCSSELADEAAYERLDSELREIDVAHI
                     RSGNRLYYLAMPPSTYLDVIQGLGGIGSEGRGGGWTRIVVEKPFGRDLQSARTLNSTL
                     AKWFDEEQVFRIDHYLGKETVQNILVFRLANAIFEPLWNSRYVDHVQITVAEEIGIEQ
                     RGAYYEEAGAIRDMIQNHVLQLLALVAMEPPITFAAGPVRDEKVKALMALRPLRGRAV
                     DEAVVRGQYSAGLSRGESASGYLQEKGVSADSTTETFVAMRVFVDSWRWAGVPFYLRT
                     "
     gene            complement(1113..2267)
                     /gene="tal"
                     /locus_tag="VFD74_09685"
     CDS             complement(1113..2267)
                     /gene="tal"
                     /locus_tag="VFD74_09685"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZK49853.1"
                     /translation="MSGNPLTQLQSLGQSVWYDNIGRDLLLSGGLKRMVDEDGVVGLT
                     SNPSIFQKAIADSDDYDVAVRTLAEAGVGAESILDTLMLEDVGMAADLLRDTFERTES
                     LDGWVSIEVAPSLAYDTAGTVAEAHRLRSLAERPNVLIKVPATAEGIPAIRQLISDGV
                     SVNVTLIFSLERYRDVMAAYFAGMERLIARRAEGEDLPQPSSVRSVASFFVSRVDSLV
                     DKLLEQAAEAAGEARSDELLGPAGRAAVANAKLAYQAFLVMFSGPRWDALSAAGALVQ
                     RPLWASTSTKNASYSDVLYVDELIGPDTVNTMPQNTLDAFRDHGTPAVTVTEGAGEAR
                     ALMAALAEAGVDIDEVTRRLEVEGVKAFADAFDSLLNTMRRKQEDMVGEG"
     gene            complement(2264..4306)
                     /gene="tkt"
                     /locus_tag="VFD74_09690"
     CDS             complement(2264..4306)
                     /gene="tkt"
                     /locus_tag="VFD74_09690"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZK49854.1"
                     /translation="MTDIDQLAVNTIRVLAIEGVEKAASGHPGLPLGAAPMAHVLWSR
                     HLKFDPRDPKWPDRDRFILSAGHGSMLLYSLLHVAGYDLSLDDLKQFRQWGSRTPGHP
                     ENGVTPGVEVTTGPLGQGFANGVGMAITERFLAATFSPDGEVVDHHVYTIVGDGDLME
                     GISSEAASLAGHLGLGRLICLYDDNHISIEGSTDLAFTENVGARFEAYDWHVQRVSDG
                     NDLEAIDRAITVARAVEDRPSLIMIRTHIGYGSPNKHDSADAHGAPLGAEEVRLTKQA
                     LGWTATEEFFVPEDVRGLYAQTAARGIALHEAWSGRLEEFRRREPEAAADFSRALAGD
                     LPAGWQEHLPVFTADVKGMATRSASGKVLNAVAGTAPTLMGGSADLAPSNNTELKECG
                     SRECGWFQKDTPGGRNVHFGVREHAMGGVLNGMALHGGVFPYGGTFLVFSDYMRPSIR
                     LAALSHARVTYVFTHDSVGLGEDGPTHQPVEHLAALRVIPNLVVIRPADANETVVAWK
                     VALERRDGPVALVLSRQNLPTLDRGVYASAAGTERGAYVLTEATGGPEAVDAIVLASG
                     SEVHVALEARAALEADGLGVRVVSMASWELFEAQGLAYQREVLPAGVRTRVAVEAAVS
                     FGWERWVGDGGSVVGIDRFGASAPGAVVLANLGITSERVVEAVKGAIDAQTGEPAA"
     gene            complement(4401..5090)
                     /locus_tag="VFD74_09695"
     CDS             complement(4401..5090)
                     /locus_tag="VFD74_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HZK49855.1"
                     /translation="MIDSNAMEGNLPNAGAIAFFDLDGTLVVGQTQRLLVSFMRREGL
                     VGIGFLVGVGVWFTAYRLGLVRATDSARAKAAELMVGRSVIETAELMDRFTREDLTPR
                     LHVGAVAALRQHQERGDEVVIVSAALMPVVEGLARHLGVRSCEGTRLETSGGTYTGSV
                     AGKALYGAEKRTVAHRILEERGADPERCYAYADHETDVDLLGLVGHPVAVSPRPGLAA
                     VARERGWQIIP"
     gene            5190..8213
                     /gene="pruA"
                     /locus_tag="VFD74_09700"
     CDS             5190..8213
                     /gene="pruA"
                     /locus_tag="VFD74_09700"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HZK49856.1"
                     /translation="MVLTAPEASANEVQTKEIGREIFSKISGQRPSIFRVDWWQGQAM
                     EWAMRDETFKVQLFRFVDVFPTLRTADEVARHLQEYFDHPSGDLPAALRWGLKAATPG
                     HATTGIAAKAIGRNIHLMAERFIAGSNARDALPTLLKLRKQRMAFTLDVLGEASLSAA
                     EALEYQRRYLDLLRDLPREVAAWPADPLLDVAPWGPLPRVNVSLKTTSLYSQIDPIDF
                     DGSREALVERLRPLFQVAAAAGVFMNLDLEQFRYRDLTYAVFEQLLMEEEFRDYPHFG
                     IVVQAYLRDAERDLRRVVELARRRGTPLTIRLVKGAYWDYETVTANQQHWPVPVFTSK
                     DDTDAAFEHLTRLLVAEHDVVHCALGTHNIRSMAAALAAVRTAGLPDSTLEFQMLHGM
                     AESVKQALVSLGFRLREYTPVGAVVPGMAYLVRRLLENTANESFLRRTFVEGADRERL
                     LSAPLPTPDLGAIPPTRLDVHPTDAAAPAPFKNTACADFSREADRARMEAALTRVRSS
                     LGVHRLLLIGGREVDTSAAITSVNPAAPRQVLGTTACARAVEAEEALIAASRAFPGWR
                     DTPPGKRAEVLFRAADLTRRRRFELAALEILEGGKTWREADADVAEAIDFLEYYGREM
                     HRLGSPRHLSDVPGETDTLFYEPRGVAAVIAPWNFPLAILAGMTSAALVAGNVVIMKP
                     ANPTPLVAWELANIFREAGLPDGVLSYLPGPGSEVGAFLVKHPQVDVVAFTGSRATGL
                     DILREAGIVRPGQRNVKRVIAEMGGKNAVIVDADADVDAAVDGVLSSAFGFSGQKCSA
                     CSRVVVVGDLYQDFVERLVRAASSIRVGDPALPGTRMGPLIDAAALRKVARYIEVGCS
                     EAELAWGSEAPPGDGYFVAPHIFVDVPPRSIIAQEEIFGPVLAIMRAPGFDEAVEVAL
                     DSDYALTGGIYSRSPRNIRRAYRDFRVGNLYVNRGITGALVGRQPFGGSRMSGVGSKA
                     GGPDYLLQFMEPRVVTENALRRGFAPTADDLTAAS"
     gene            8250..>8722
                     /locus_tag="VFD74_09705"
     CDS             8250..>8722
                     /locus_tag="VFD74_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZK49857.1"
                     /translation="MEHPPSHGLHDLDLKRVELERKVVTLLSRTAASNTNELHDEHLT
                     LGDRMADRLAAVAGSWRFIGGFAVVLGVWIAVNGAAFSFRWDPYPFILLNLVLSCIAA
                     IQAPIIMMSQSRLESRDRLRGENDYAVNVKAEILLEHLTAEVETIKSMLMGMGAAL"
BASE COUNT         1426 a         3045 c         2797 g         1454 t
ORIGIN      
        1 agtgcgtagg tagaagggca cgccggccca gcgccagcta tccacgaaca cgcgcatcgc
       61 cacgaaggtc tccgtggtcg agtcggcgga cacccccttc tcttggagat acccagacgc
      121 tgactcgccc cgagagaggc cggccgagta ctgtccccgg acgaccgcct cgtccaccgc
      181 gcggccgcgc aggggccgca gagccatgag cgccttgacc ttctcgtcgc ggaccgggcc
      241 ggcggcgaag gtgatcggcg gctccatggc gacgagggct aggagttgca gaacgtggtt
      301 ctggatcatg tccctgatcg cgcccgcctc ctcgtagtag gcgccgcgtt gctcgatgcc
      361 gatctcctcc gccaccgtga tctgcacgtg atccacgtag cgactgttcc acagcggctc
      421 gaagatggcg ttagccagcc tgaagacgag gatgttctgg actgtctcct tgccaaggta
      481 atggtcgatc ctgaacacct gttcctcgtc gaaccacttg gccagggtgc tgttgagcgt
      541 acgtgcgctc tgcaagtcgc ggccgaaggg cttctccacg acgatgcgcg tccatcctcc
      601 gcctcggcct tcggatccga tcccgcccag gccctggatc acatcgagat acgtgctcgg
      661 aggcatggcc aggtagtaga ggcggttgcc ggaacggatg tgtgccacgt cgatctcgcg
      721 caactcgctg tcgaggcgct cgtacgctgc ttcgtcggcg agttcactcg aacagaaatg
      781 gagcgtctcg gcaaaccctc tccacatctc ctcctgcacc ggcgcgccca ggtgctcgtc
      841 caaagcgcct ctgaccctct gctggaactc cagcctatcc cacgctcggc ggcccacgcc
      901 gacgatggcg ataccgggag gcagaagacc ccgtacgtag agccggtaga gggacggcat
      961 caacttgcgg ttggagaggt cgccggtgac cccgagtatc accagtgacg ccggtggcgg
     1021 ggtccggcgc agccgcatcc ccactcgaag agggttctcc aggaaccgcg gcgcgctcat
     1081 ggttgtgagg cggaggaggt gaccgtggcg gcctagccct cccccaccat gtcctcctgt
     1141 ttgcgtcgca tcgtgttcag caacgagtca aaggcgtcgg cgaacgcctt gacgccctcg
     1201 acctccaggc ggcgcgtcac ctcgtcgatg tcgactcccg cctccgcgag ggcggccatg
     1261 agggcgcggg cttcgcccgc gccctcggtg accgtgaccg caggcgtgcc gtggtcgcgg
     1321 aaggcgtcga gcgtgttctg cggcatggtg ttcaccgtgt ccggcccgat gagttcgtca
     1381 acgtagagca cgtcgctgta tgacgcgttc ttcgtgctcg tgctggccca caggggacgc
     1441 tgcacgaggg ctccggcagc ggacagcgca tcccaacgcg ggcccgagaa catgaccagg
     1501 aacgcctggt aggccaactt tgcgttggcc acggctgctc gccctgcggg gccgagcagc
     1561 tcgtcgctcc gggcttcgcc ggcggcctcg gcggcctgct cgagcagctt gtccaccagt
     1621 gagtcaacgc ggctcacgaa gaagctggcc acgcttctca ccgacgacgg ctgcggcagg
     1681 tcctccccct cagcccgccg cgctatcagt ctctccatgc ccgcgaagta ggcggccatg
     1741 acgtcgcggt agcgctctaa ggagaagatg agcgtcacgt tgacgctcac gccgtcgctg
     1801 ataagctgcc gtatggcggg gatgccttcc gccgtcgccg ggaccttgat gagcacgttg
     1861 ggccgttccg ccagcgaccg cagacgatgg gcttcggcga ccgtgccggc cgtgtcgtac
     1921 gccagcgaag gcgcgacttc gatgctcacc cagccgtcga ggctctccgt gcgctcgaag
     1981 gtatcccgca ggagatccgc tgccatgccg acatcctcga gcatcaaggt gtcgaggatg
     2041 gactccgctc ccacgccggc ctcggccaac gttctgacgg ccacgtcata gtcgtcgctg
     2101 tcggcgatgg ccttctggaa gatgctcggg ttggaggtga ggcccaccac cccgtcctcg
     2161 tccaccatcc tcttcagccc tccggagagg agcagatcac ggccgatgtt gtcgtaccac
     2221 acgctctgcc ccaggctctg aagctgggta agcggatttc cgctcatgcc gccggttctc
     2281 ccgtctgggc gtcgatagcc cctttgactg cttccaccac gcgctcgctc gtgatcccca
     2341 ggttggccaa gaccacagcc ccaggggcgg aggctccgaa ccggtcgatg ccgaccacgg
     2401 accctccatc gccgacccat ctctcccagc cgaaggagac cgccgcctcc acggccacac
     2461 gcgtgcgcac gccggcaggc agcacttctc gctgatacgc gagtccttgc gcctcgaaga
     2521 gttcccagct cgccatggag accacacgca ctcccaggcc gtcggcctcc agcgccgcgc
     2581 gcgcctcgag cgccacatgc acctccgagc cgctcgccag cacgatggcg tccaccgcct
     2641 cagggccgcc ggtcgcctcc gtaaggacgt aggcgccgcg ctccgtgccc gccgccgacg
     2701 catagacccc gcggtcgaga gtaggcaggt tctggcgact cagtacaagc gccacaggcc
     2761 cgtcgcgccg ctcgagcgcc accttccacg caacgacggt ctcgttggcg tcggcgggcc
     2821 ggatcacgac taggttgggg atcacgcgta gggccgccag gtgttccacg ggctggtgcg
     2881 tgggaccatc ctcgccgagg cccacgctgt cgtgtgtgaa cacgtaggtg acccgagcgt
     2941 gggagagcgc ggccaaccgg atcgatggcc gcatatagtc ggagaacacg aggaaggtgc
     3001 cgccgtatgg gaacactccc ccgtggagcg ccatcccgtt gagtaccccg cccatcgcgt
     3061 gctcccgcac tccgaagtgc acgttgcgcc cgccgggggt gtccttctgg aaccatccgc
     3121 actcccgcga gccgcactct ttgagctcgg tgttgttcga aggcgccagg tcggccgagc
     3181 cgcccatcag ggtgggggca gtgccggcga ccgcgttcag caccttgccc gaagccgacc
     3241 ttgtggccat gcccttcacg tccgccgtga acaccggcag gtgctcctgc caaccggccg
     3301 gtaggtcgcc ggcgagcgcc cgggagaagt cagcagccgc ctccggctcc cgccgtcgga
     3361 actcctcgag tctgcccgac caggcttcgt gcagagcgat gcctcgggct gccgtctgcg
     3421 cgtagagacc gcgcacatcc tcgggcacga agaactcctc ggtagccgtc cagcccaagg
     3481 cctgcttggt gagccgcact tcctcggcac ccaagggagc gccgtgcgcg tctgcggaat
     3541 cgtgcttgtt ggggctaccg tagccgatat gggtgcggat catgatcagg gaaggccggt
     3601 cctccaccgc ccgggccacg gtgatggcac ggtcgatcgc ctcgaggtcg ttgccgtcgg
     3661 agaccctctg cacatgccag tcgtaggcct cgaaacgcgc tcccacattc tcggtgaaag
     3721 caaggtcggt ggagccctcg atggatatgt gattgtcgtc gtagaggcag atcagccgcc
     3781 cgaggccgag gtgacccgcc agggaggcgg cctcggagga gataccttcc atcaggtcgc
     3841 cgtcacccac gatcgtgtac acgtgatgat ccacgacctc gccgtctgga gagaacgtcg
     3901 ccgccaggaa ccgctcggtg atcgccatgc ccacgccgtt cgcgaagccc tgacccagcg
     3961 gccccgtggt gacctccacc cccggcgtga ccccgttctc ggggtgtccc ggggtgcgac
     4021 tcccccattg gcggaactgc ttcaagtcgt cgagcgaaag gtcgtagccc gccacgtgga
     4081 gcagggagta gaggagcatc gacccgtgtc ccgcgctgag gatgaagcgg tcgcgatcgg
     4141 gccacttcgg atcgcgtgga tcgaacttca ggtgccgaga ccagaggacg tgagccatgg
     4201 gcgccgcacc gagcggcaga cccgggtggc cggaagcggc cttctcgacc ccctctatgg
     4261 ccagaacccg tatcgtgttc acggccaact gatcgatatc agtcacgtca ctcgcacctt
     4321 tcttcgtcac gtcaccatgc actcgtcact tctacccggc ccctggcccc gccggaaccc
     4381 gaaggggtac gcgtgaggcc ctacgggatg atttgccacc ctcgttcccg cgcgaccgcc
     4441 gccaggccgg gccgcgggct caccgccacc gggtggccga cgagtcccag aaggtccacg
     4501 tcggtctcgt ggtcggcgta ggcgtagcac cgctccgggt ccgcaccgcg ttcctcaaga
     4561 atccggtggg ccaccgtccg cttctcggcc ccgtacaatg ccttgccggc gaccgagccc
     4621 gtgtaggtgc ccccgctcgt ctccagccgg gtgccctcgc aggaccgcac accgaggtgc
     4681 cgggccaacc cctcgaccac aggcatgaga gccgcggaga cgatcaccac ctcgtcgccc
     4741 cgctcttggt gctgccgaag cgccgcgacc gcccccacat gcaggcgggg ggtcaagtct
     4801 tcgcgggtga acctgtccat cagctccgct gtctcgatca ccgaccggcc caccatcagc
     4861 tccgccgcct tggcgcgagc gctgtccgta gccctcacga gaccaaggcg gtacgccgtg
     4921 aaccacactc ccacgcccac caggaatccg atccccacca ggccctcccg gcgcatgaag
     4981 gagaccagga gccgctgcgt ctgccccacg accaaggtgc cgtcgagatc gaagaatgcg
     5041 atcgctccgg cgttcggcag gttcccttcc atggcgtttg agtctatcac gttgctattg
     5101 gccgacttcg gggtagaggc acccccgcga caggtaggga ggccgccgag accctgcagt
     5161 gcacggcggc ccgtagttcg gaggtcgaca tggtgctcac ggcccctgag gcatcagcta
     5221 acgaggtcca gaccaaggag atcggtcgtg agatcttctc gaagatctcc ggtcaacgac
     5281 cctccatatt ccgggtggac tggtggcaag gccaggccat ggaatgggcc atgcgcgacg
     5341 aaaccttcaa agtgcagctt tttcggttcg tcgacgtgtt cccgacgctg cgcaccgcgg
     5401 acgaggtcgc gaggcacctg caggagtact tcgaccaccc cagcggcgac ttgccggcgg
     5461 cgttgcggtg gggattgaag gcggcgacgc ccggccacgc cactaccggg atcgcagcca
     5521 aggccatcgg gcgcaacatc catctcatgg ccgagcggtt catcgccggg agcaacgcgc
     5581 gggacgctct ccccacgctc ctcaagttga ggaagcagcg gatggccttc acgctcgacg
     5641 tgctcggcga ggcttcgctc agcgctgctg aagcactcga gtaccaacgc cggtacctcg
     5701 atcttctgcg cgacctcccg cgggaggtcg cggcttggcc ggccgatcca ctcctggacg
     5761 tggctccgtg gggtccgctc ccgcgggtga acgtgtcact caagaccacc tccctctact
     5821 cccagatcga cccgatcgac ttcgacggtt cgagggaagc cttggtcgag aggctgaggc
     5881 cgctcttcca ggtagccgct gcggccgggg tcttcatgaa cctcgacctc gagcagttcc
     5941 gttatcgcga cctcacgtac gcggtcttcg agcagctgct catggaagag gagttccggg
     6001 actacccgca cttcggcata gtcgtgcagg cctatctgcg tgacgccgaa cgcgatctgc
     6061 gaagagtcgt ggagttagcg cgccgccgcg gcacgcccct cacgatacgg ctcgtcaagg
     6121 gcgcttactg ggactacgag accgtgacgg ccaaccagca gcattggccg gtgccggtgt
     6181 tcaccagcaa ggacgacaca gacgctgcct tcgaacatct cacacgtctg ctggtggccg
     6241 agcacgacgt cgtccactgc gccttgggaa cgcacaacat ccggagcatg gccgcggccc
     6301 tcgccgcggt gagaacggcg ggtctgccgg attccaccct cgagttccag atgctgcacg
     6361 gcatggcgga atcggtcaag caggcgttgg tctccctggg gttccgcctg cgcgagtaca
     6421 ccccggtggg ggcggtggtc cccggaatgg cctatcttgt gcgccgtctc cttgagaaca
     6481 ccgcgaatga gtcgttcctc cgccgcacct tcgtcgaggg agcggatcgt gagcgtctgc
     6541 tctccgcgcc gctcccgacc cccgacctgg gcgccattcc gccgacccgg ttggacgtgc
     6601 accccaccga tgccgcagcg cccgctccgt tcaagaacac agcttgcgcc gacttctcgc
     6661 gagaggcgga ccgcgcccgc atggaagccg ccctcacgag ggtgcgaagc tctctgggcg
     6721 tgcaccggct tctgctcatc gggggacggg aggtggacac cagcgcggcg atcacctctg
     6781 tgaacccggc cgcgccgcgg caggtgctcg gcaccacggc gtgcgcccgc gcggtggagg
     6841 cggaggaggc tctgatcgcg gcctcacggg cctttcccgg ttggcgtgac accccgcccg
     6901 gcaagagggc cgaggtgctc ttccgcgcag ccgacctcac gcggcggcgg cgtttcgagt
     6961 tggccgcgct cgagatcctg gagggcggaa agacctggag ggaagccgac gccgacgtgg
     7021 cggaggcgat cgacttcctc gagtactacg gccgcgagat gcaccggctc gggtccccac
     7081 gccacctctc ggatgtcccc ggcgagaccg acacgctctt ctacgagccc cgaggagtgg
     7141 ctgcggtcat cgccccttgg aacttcccgc tcgccatcct ggccgggatg acctctgcgg
     7201 cgctcgtcgc gggcaacgtc gtcatcatga agccggccaa tcccaccccc cttgtcgcgt
     7261 gggaacttgc caacatcttc cgagaggccg gtcttcccga cggggtcctc tcttacctcc
     7321 ccggccccgg ctctgaggtc ggcgccttct tggtcaaaca ccctcaggtg gacgtggtgg
     7381 cgttcacggg cagccgcgcg accgggctcg acatcctgcg cgaggcgggg atcgtcagac
     7441 ccggccagcg gaacgtcaaa cgggtaatcg cagagatggg cggcaagaac gcggtgatcg
     7501 tcgatgcgga cgccgacgtg gacgcagcgg tcgacggcgt cttgagctcc gctttcggct
     7561 tctccggcca aaagtgctcg gcctgcagcc gagtggtggt ggtgggcgac ctctaccagg
     7621 acttcgtgga acgcctcgtg cgggcggctt cgagcatccg ggtgggtgac cccgcactcc
     7681 cgggcacgcg catgggcccg ctgatcgacg ccgcggccct caggaaggtc gcgcgataca
     7741 tcgaggtcgg atgctcagaa gcggagttgg cgtggggctc ggaagccccg cctggggacg
     7801 gctacttcgt ggcacctcac atattcgtgg atgtcccacc tcgctcgatc atcgcccaag
     7861 aggagatctt cggaccggta cttgcgatca tgcgcgctcc cggcttcgac gaggctgtcg
     7921 aggtggcgct ggacagcgac tacgcgctca ccggaggcat atactcgcga agtccgcgga
     7981 acatcaggcg cgcctaccgc gacttccgcg tcggcaatct ctacgtgaac cgggggataa
     8041 ccggggcctt ggtaggccgc cagccgttcg gtggttccag gatgagcggc gtcggctcca
     8101 aggccggcgg ccccgactac ctgctacagt tcatggagcc tcgcgtagtg acggagaacg
     8161 ccctgcgtcg aggcttcgcg ccgaccgcgg acgatctcac ggcggcctcg tgatcgagca
     8221 ccgcgcccca actgcgaagg gggtctagag tggagcaccc gccatcccac ggcctgcacg
     8281 acctggatct gaagagggtc gagctcgaga ggaaggtggt gaccctcctc tcccgcaccg
     8341 ccgcctccaa caccaacgaa ctccacgacg aacacctcac gctcggcgat aggatggccg
     8401 atcgcctggc cgccgtggca ggcagctggc gcttcatcgg cgggttcgcg gtcgtcctgg
     8461 gagtctggat cgcggtcaat ggtgcagcat tctccttccg ctgggatccg taccccttca
     8521 tcctgttgaa cctcgttctc tcgtgcatag ctgccatcca agcgcccatc atcatgatga
     8581 gccagtcgag gctggagtcg cgcgaccgcc tgcgcggtga gaacgactac gccgtcaacg
     8641 tgaaggctga gatactgctc gaacatctga ccgcggaagt cgagaccatc aagtccatgc
     8701 tgatgggcat gggcgccgcg ct
//
LOCUS       DASRMJ010000350         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_358863, whole genome shotgun sequence.
ACCESSION   DASRMJ010000350 DASRMJ010000000
VERSION     DASRMJ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358863"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFD74_09710"
     CDS             <1..546
                     /locus_tag="VFD74_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49858.1"
                     /translation="GGDKANTSITMVTRMVLTVLVTTPGPAVPELKISGVKAEARPDG
                     LYVIVAIANDGTAPTSGEGTISMPSEGFQQKIALGDMVPASSTGYPIKWKTDPVAGTY
                     PAQAEVRYAGGTKVAKWSGEFTVVDANLAELKDRLVAPEAATVAAAGPPWLMYGLIGG
                     LIVVVLIMGIALLRRRPRPAS"
     gene            694..1919
                     /locus_tag="VFD74_09715"
                     /pseudo
     CDS             694..1919
                     /locus_tag="VFD74_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    969..1085
                     /locus_tag="VFD74_09715"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          412 a          663 c          656 g          350 t
ORIGIN      
        1 ggcggcgaca aagcaaacac tagtatcacc atggtgacgc gcatggtgct gacggtcctg
       61 gtcaccaccc cgggcccagc agtgcccgag ctcaaaatca gcggggtcaa ggcagaggcg
      121 cgccccgacg gcctgtacgt gatcgtcgcc atagccaacg acggcaccgc gccgaccagc
      181 ggcgagggca cgatcagcat gcccagcgag ggcttccagc agaagatcgc ccttggggac
      241 atggtcccgg cctcgagcac cgggtacccc atcaaatgga agacggatcc ggtggcgggt
      301 acctacccgg cccaggcgga ggtccgctat gccggcggga ccaaggtcgc caagtggagc
      361 ggagagttca ctgtcgtcga cgcgaacctg gctgaactga aagatcgcct ggtcgcgccg
      421 gaggccgcga ccgtagcggc cgctggccca ccctggctga tgtacgggct gataggcggt
      481 ctgatcgtcg tggtcctcat catgggtatc gcactcctgc gccgccgccc caggcccgcg
      541 tcttagaagc ggggccgtcc aacagcccgg cgacggcctc atcgccgggc tgagcagcct
      601 gccgaagcgc cggggcgatc tcttcctcgc gggtccgtga gagtgaaccg ccccgccttt
      661 gccggagact ccaacctttg agaggatgga gtcatgaaca cacaaagaag gtatccacgt
      721 gaggtgaagg aacgggccgt tcgcatggtg ctcgaacacg aaggagagta cgactcccag
      781 tgggcggcga tcagctcggt ggcggcgaag ttgggcatga cagcggagac gctccgcaag
      841 tgggtgcgcc gagcagagac tgacggcggt ctgcgctccg gggtcacgac cgaggagcgg
      901 gcccgggtca aggagctcga gcgggagaac cgtgaactcc ggcgcgccaa tgagatcctg
      961 aaagcagccg cagctttctt cggggcggag ttcgaccgcc gacagaagag atgaccgcct
     1021 tcatccagga acgcaaggaa gagttcgggg tcgagccgat ctgcaagatg ctgccgatcg
     1081 ccccttccac ctactacgcc gtgaagaccc gccccccgtc agccaggcag ctccgcgacg
     1141 aggagctgaa gcccgagatc atccgcgtct acgaagagaa ccaatccgtc tacggccccc
     1201 gcaaggtgtg gcggcaactg aagcgggaag gctttcagac ggcccgctgc acggtcgagc
     1261 gcctcatgcg cgctctggag atcaaaggtg aggtcagggg caagacctgg aagaccacct
     1321 atcccgacct ctccgccccc cgtcccttgg atctcgtgga ccgcgacttc acggctgcgg
     1381 cccccaacag gctatgggtg gcggatctga cctacgtccg cacctggtcg gggttcgtct
     1441 acgtcgcctt tgtcatcgac gcctactccc gcatgatcct gggctggcag gtctccgcct
     1501 ccctgcgcac cgatctggcg ctcgatgctc tggagatggc cctctggcgg cgtggtggcg
     1561 atgttgaggg cctcgttcac cactcagaca ggggcggtca atacctctcc atccgctaca
     1621 ccgaccgcct ggaggacgcc ggcgcactca cctcagtggg ctcccgcggg gatgcctacg
     1681 ataatgccct ggccgagacc atcaacggcc tctacaagac cgaggtcatc agaaagcgcg
     1741 gcccctggaa gaccctcgaa gacgtggagt tcgccacctt agaatgggtg gattggttca
     1801 acaaccgccg acttcttgag tacaccggct acctcccccc ggcagagttc gaagagatgt
     1861 cctatcgtga gcactccctc gttgaagacg agggactcaa gcaaatgagt ctccagtgaa
     1921 cccggggcgg ttcaacctgc aagacaagat cacatagccg ctttgatatc tgtcgatgct
     1981 acgtgtatcc ttattgttag tttatgtgtt ctcgatactg ttgtgcgaga actcgcacaa
     2041 ggaggctacc tatggctcgc agtagaggtt tctccctcgt t
//
LOCUS       DASRMJ010000351         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_252016, whole genome shotgun sequence.
ACCESSION   DASRMJ010000351 DASRMJ010000000
VERSION     DASRMJ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252016"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VFD74_09720"
     CDS             <1..1265
                     /locus_tag="VFD74_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK49859.1"
                     /translation="MMLGEGKGLRLDPKSAAEALARFGSMLGVEFDPGAEIGRLALGR
                     RQQMEIAKALWSGSTVLMLDEPTAMLTPQGAEELARVLVRLARRGLAIVFVTHKLHEA
                     LEVGDRITVLRRGRVTGAWGPDEISAAGRETMRRRIVEAMFGGGVAPEVQIGRSAHPG
                     RRGLRAGAHARPALELDRLCVEPRRGEVGVRDVTLTVWPGEIVGVAGVDGNGQRELAE
                     AVAGQRSVSSGEIRVGGIRVTGDDVSGRMRRGLRYVTDDRIGEGTVPSLSVALNLILK
                     RIGEKPYWRHGRADEKLIEENARELVERFDIRTPGVGARCGTLSGGNLQKVVLARELS
                     SGPAVVVYSKPTHGLDARTTMTVRDRITALAEEGAASLLISSDLDEILDLSHRIAVIF
                     GGRLTGIVDNVPGAEREVGDLMVGGSAR"
     gene            1262..2326
                     /locus_tag="VFD74_09725"
     CDS             1262..2326
                     /locus_tag="VFD74_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49860.1"
                     /translation="MKGIVRVGGPILLALLAGGVLLVALGRDPFSFYADILSRGLLDP
                     SGVQDTLVRMAPLLLMASGLIIAFRANIWNIGGDGQFLLAAAAVAGLGPWLIGVLPNV
                     LAFLILGLIGATVGAAWTLVPAYLKANFGVNEIVTSLMLTFVGVNLGNLLIKGPFRST
                     KTLVPQTDVIPFRELLPKIPGTRIHVGLLLALLAILIVHYTMTRTSFGLRLRILGSSP
                     RAAAHIGLHTARLTMISFALSGAFMGLAAAVEILGVWGYMRADWNPGFGLPLFALVFL
                     ARLDAVGVIPLVGFYGVISMGGHYGARRADLPDDFMLVIVGMILLFMALAEYFASRGD
                     ERPSLAGGLRAALRRGSGRG"
     gene            2319..3254
                     /locus_tag="VFD74_09730"
     CDS             2319..3254
                     /locus_tag="VFD74_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK49861.1"
                     /translation="MGDLLTAAFVSALLSGGVMAGMPLLLASLGEAISERAGVLNIGL
                     EGMMLAGAYAGFVAVLLTGSHWLGLGAGLAAGMAVSVLMVGLCVRLGLSQIVVGIAIL
                     LTAEGVTSVLHRAQFGTTYPRLGSMAVLQLPVLSGIPVVGASLFSQPAPVYAGLGLVV
                     AAWWVLRSTNFGLELRAAGEKPEALDAAGVSVVRTRSLAVLCAGALAGLGGAYMSIIG
                     AGIFVPFMTHGAGFIAIVLAMLARGRAAWVVIGSFAFGMTVSLATALQLVGVFVPIDL
                     VNMLPFVVVLVALVIFARRAYLPSALGLPYQRGSR"
     gene            3255..4043
                     /locus_tag="VFD74_09735"
     CDS             3255..4043
                     /locus_tag="VFD74_09735"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZK49862.1"
                     /translation="MNRRKGGIPLYYQVMRSLKEQVLSGRLPPGERIPSESELTAAFG
                     VSRVVVRQALRILEDEDLIFRVKGKGTFVADGVDVAKSPRLWGYLEDLISTGFAVTVS
                     VLEFGLRKATAELAPVFDTAEGSDLFFMKRLRLVDGRPFSVIENHLPVDVGKCIPLEA
                     LESEPLMRLIETRAGVSVDWVAQVFQAVAADAELAALLEIDVVSPVLKMILTAHSADG
                     RVLDLANVYFRSDRYNYRAHLKRRGTGEKAGWVPMAVEHAGKLR"
     gene            4024..>4622
                     /locus_tag="VFD74_09740"
     CDS             4024..>4622
                     /locus_tag="VFD74_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49863.1"
                     /translation="MRASFGRAFGLVAACAVWLAAGCGAPAEVGRATAPAVTFQSTFA
                     REYARLHGATDAGILSAVAATQAEIAAALRAGYTGDVLLLPSTLPVGFALAAPFRGTG
                     SGGPLPNPHAWGSGYAVTYTDGRGRLTLMVNPDEPVEGGAWERTTLTLGGAALVVQER
                     GGLVLVSTTSAAPLGAEPPTTSAAPLGAGLPTTSEAPLGA"
BASE COUNT          618 a         1511 c         1628 g          865 t
ORIGIN      
        1 acatgatgct cggcgaggga aagggcctgc gcttggatcc caagtcggcg gcggaggcgc
       61 tggcccgctt cgggtcgatg ctgggcgtcg agttcgaccc gggcgccgag atcggccggc
      121 tggctctggg gaggcgccag cagatggaga tcgcgaaggc cctgtggtcc ggatccacgg
      181 tcctgatgct cgacgagccg accgccatgc ttactccaca aggagccgag gagttggctc
      241 gagtgcttgt caggttggcg cggcggggcc tcgccatcgt gttcgtgacc cacaagctgc
      301 acgaggcctt ggaggtgggc gaccgcatca ccgtgttgag gcgcggccgc gtgaccggtg
      361 cgtgggggcc ggacgagatc tccgcggcgg gtcgcgagac catgcgtcgg cggatcgtcg
      421 aagccatgtt cggcggcggt gtcgcccccg aggtgcagat cgggcgatcc gcacatccag
      481 gccgccgggg ccttcgtgcc ggcgcgcacg cccggcccgc actcgagctg gaccgactct
      541 gcgtggagcc gcgccgcgga gaggtgggcg tccgcgacgt caccttgacc gtgtggcccg
      601 gggagatagt gggcgtcgct ggagtcgatg gcaacggcca gcgcgaactg gccgaggcgg
      661 tggccggtca gcggtccgtc tcgtccgggg agatccgagt gggcggtatc cgcgtgaccg
      721 gcgacgacgt gtccggccgc atgcgccgcg gcctgcgcta cgtcaccgac gatcgcatcg
      781 gggagggcac cgttccctcc ctgagcgtcg ccctgaatct catcctcaaa cggataggcg
      841 agaagcccta ttggcgtcac ggccgtgcgg acgagaagct catcgaagag aatgcccgtg
      901 aactcgtcga gcgtttcgac atccgtacgc ccggggtggg tgcgcgctgc ggaacgctct
      961 ccggaggcaa cctccagaag gtcgtgctcg cccgggagct gtcgtcgggt cccgcggtcg
     1021 tggtctacag caagcctacg catgggctgg acgccaggac gacgatgaca gtgcgcgacc
     1081 ggataacggc cctcgccgag gagggggcgg catcgctcct gatctcctcc gacctcgacg
     1141 agatcctcga tttgagccac cgcatcgctg tcattttcgg cgggaggctc accgggatcg
     1201 tcgacaacgt tcccggtgcc gagcgtgagg tcggcgatct catggtgggg gggtcggccc
     1261 gatgaagggc atcgtacgag tcgggggccc gatcctcctc gccctgctgg ccggtggtgt
     1321 cctgctggtc gctctcggga gagacccctt ctccttctac gcggatatct tgagccgggg
     1381 tcttctagac ccgagcggtg tccaagacac gctggtgcgc atggctcctc tgctgttgat
     1441 ggcctcaggt ctgatcatcg ccttccgcgc gaacatctgg aacatcggcg gcgacgggca
     1501 gttcctgctg gcggctgccg cagtcgccgg tctgggtccg tggctgatcg gtgtcctgcc
     1561 gaacgttctc gccttcctga tcctgggcct catcggcgcg accgtgggcg ccgcgtggac
     1621 cttggttccg gcctatctca aagccaactt cggagtgaac gagatagtaa cctcgctgat
     1681 gctcacgttc gtgggggtga acctgggcaa cctcctcatc aagggcccgt tccgcagcac
     1741 gaagaccctc gtcccgcaga cggatgtgat cccctttcgt gagttgctgc ccaagatccc
     1801 gggcacccgc atccatgtcg ggctgctgct cgcgctgctc gcgatactca tcgtgcacta
     1861 cacgatgacc aggacatctt tcggcctcag actcaggatc ttgggctcca gcccgcgagc
     1921 ggccgctcat atcggtctgc ataccgcgcg actcaccatg atcagcttcg cgctcagcgg
     1981 tgccttcatg ggtctggccg ccgcggtgga gatactggga gtgtgggggt acatgcgcgc
     2041 ggactggaat cccggcttcg ggctacctct gttcgcgctc gtcttcttgg ccaggctgga
     2101 tgccgtgggc gtgatcccgc tcgtcgggtt ctacggagtg atctcgatgg gtggccacta
     2161 cggcgcccgg cgcgccgacc tccctgacga cttcatgctg gtgatcgtgg gcatgatcct
     2221 gctcttcatg gccctggccg agtacttcgc ttcgcgcggc gacgagcgcc cctctctagc
     2281 cggggggttg cgtgcggcgc tgagacgggg gtccggccgt gggtgacctg ctgacggccg
     2341 ccttcgtgtc ggcactcctt tccggaggcg tgatggcggg catgccgctg ctgctggcga
     2401 gtctggggga ggccatctcc gagagggcgg gcgtcctcaa catcgggttg gaagggatga
     2461 tgttggctgg ggcgtacgcc gggttcgtcg ctgtgctcct caccggctcg cattggctgg
     2521 gcctgggcgc ggggctcgca gcggggatgg ccgtctccgt gctgatggtc ggcctctgcg
     2581 tccgactcgg cctcagccag atcgtggtgg ggatcgccat cctcctcacc gccgaaggcg
     2641 tcaccagcgt gctgcatcga gcccagttcg gcacgacgta tccgcgactc gggtcgatgg
     2701 ccgtgctcca gctccccgtg ctttccggga taccggtcgt gggggcgagc ctcttctcgc
     2761 agccggcgcc cgtgtacgcg ggcttggggc tggtcgttgc ggcatggtgg gtgctccgtt
     2821 ccacgaactt cggcctggag ttgcgcgccg ccggggagaa gcccgaggcg ctcgacgccg
     2881 cgggcgtgag cgtggttcgc acgcgatcgc tggccgtcct ctgcgccgga gcactcgcgg
     2941 gcctgggcgg ggcctacatg tcgatcatcg gagccgggat attcgtgcct ttcatgaccc
     3001 acggagccgg gttcatagcc atcgtccttg ccatgcttgc tcgggggcgc gccgcgtggg
     3061 tggtaatcgg gtcgttcgcc ttcgggatga cggtgtctct ggcgaccgcg ctccaactcg
     3121 tgggagtgtt cgtccccatc gatctcgtga acatgctgcc gttcgtggtg gtgctcgtcg
     3181 ccctcgtgat cttcgcccgg cgggcctatc tgccctccgc gctggggctt ccctatcaac
     3241 gcgggagtcg atagatgaac agacgcaagg gcggcatacc tctgtactat caagtgatgc
     3301 gttcgttgaa ggagcaggtg ctctccgggc gactgcctcc aggtgaacgt atcccgtcgg
     3361 agtccgagct caccgcggcc ttcggcgtga gccgcgtggt cgtgcgtcag gcccttcgga
     3421 tcctggagga cgaggatctc atcttcaggg tcaaggggaa gggcaccttc gtggctgacg
     3481 gggtggacgt ggcgaagagc ccgcggctct ggggatacct ggaggatctc atcagcaccg
     3541 gcttcgcggt gaccgtcagt gtcctcgaat tcggtctacg gaaggcgacc gccgagttgg
     3601 cgcccgtgtt cgacacggca gagggctcgg acctgttctt catgaagcgc ctgcgtcttg
     3661 tggacggccg tcccttctcg gtcatcgaga accacctgcc ggtggatgtc ggcaagtgca
     3721 tcccgttgga ggcgctggag agtgagccgc ttatgcgctt gatcgagacg cgcgccggtg
     3781 tgtccgtcga ctgggtggca caggtgttcc aggccgtcgc ggcggacgcg gaactcgccg
     3841 cgcttctgga gatcgacgtg gtatccccgg tcctcaagat gatcctcaca gcgcactcgg
     3901 ctgacggccg ggtgcttgat ctagccaatg tgtacttccg ctccgaccgc tacaactacc
     3961 gtgctcacct caagcggcgc ggcacgggcg agaaggccgg atgggtgccc atggcggtgg
     4021 agcatgcggg caagcttcgg tagggctttc ggcctcgtcg cagcctgcgc agtgtggttg
     4081 gccgccggct gcggcgcgcc cgccgaggtc gggcgcgcca ctgcgccggc ggtgaccttc
     4141 cagagtacgt tcgctcggga atacgcccgc ctccacggcg ccaccgacgc cggcatcctg
     4201 tcggcggtcg ctgcgaccca ggcggagatc gcggcggcgc tgcgcgccgg ctacacggga
     4261 gacgtccttc ttctcccttc gactctgccc gtcggcttcg cgctcgccgc ccccttccgc
     4321 ggcacggggt cgggagggcc cctgccgaac cctcacgcct ggggctccgg ctacgcggtc
     4381 acctacaccg acgggcgtgg gcggctcacc cttatggtga accctgacga gccggtggag
     4441 ggcggagcgt gggagcgcac gaccttgacg ctcggtggcg cagccttggt ggtccaggag
     4501 cgcggcggtc tcgtgctggt gtccaccacc tccgccgcgc ccctcggtgc cgaaccgccc
     4561 accacctccg ccgcgcccct cggcgccggc ctccccacta cgtccgaggc gcccctcggt
     4621 gc
//
LOCUS       DASRMJ010000352         5009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_359211, whole genome shotgun sequence.
ACCESSION   DASRMJ010000352 DASRMJ010000000
VERSION     DASRMJ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359211"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..784
                     /gene="rnhA"
                     /locus_tag="VFD74_09745"
     CDS             356..784
                     /gene="rnhA"
                     /locus_tag="VFD74_09745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009543553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZK49864.1"
                     /translation="MRAFTDGACLGNPGPGGYGAILIYRGRRKELSGGEPATTNNRME
                     LRACIAALRALKHPCDVELTSDSRYVVQGIEDGWAERWRRNGWMRTKKDPAKNADLWQ
                     ELLDLCAIHRVRFLWVRGHDGHPENERADELAVAAARQAG"
     gene            complement(872..2137)
                     /locus_tag="VFD74_09750"
     CDS             complement(872..2137)
                     /locus_tag="VFD74_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49865.1"
                     /translation="MQLDREALRIAPGRLDEVNALLTDPANPTVSALLEIVEKHGGPE
                     EINRKAGEAGRLDNLLARLEAEHSPYLAHLEWLTERRDSGAFVSLAEYRSRTLAGQAG
                     PEDVDDANAVTLEISAMQYFPWLIAEARQAIERRELMPGRFIRVRNMAEQTADQGDIL
                     ATAAAMQIVGASYVETLDTKGTDGSNVHLGGPDTITGYFGGVGQPNEHAIAWVDEYLR
                     YFTTYGVRQVLNVNPGTVMAAYLLHKLGVDVEFKISVFMGNDNPWSILWTLLTARLFS
                     RDDGSTPLVGFNLSNSVDNETIRQASYLRRTLGLEKAVRFEHHVTEAYRSIVIQPYLR
                     RAELVEIAREVPNISAKHEGGDPEVEAGRAHPSDILDYFATKAAVMEQDLMPALERNY
                     LDKHEAVNRTAEALIRAGVGVVCAERLHA"
     gene            complement(2240..3082)
                     /gene="panB"
                     /locus_tag="VFD74_09755"
     CDS             complement(2240..3082)
                     /gene="panB"
                     /locus_tag="VFD74_09755"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZK49866.1"
                     /translation="MAKKTIHEFYAMKATGEKATFLTAYDFPTAQFAEAAGLDMLLVG
                     DSLGMCVYGYSGTIPVTMDQCIVHADAVRRGAPNTFVIGDMPFMSYQSSTEKAVENAG
                     RFLKEAGVDAVKLEGGVRVAPQIRAIVDAGIVVMGHIGLTPQSSGQLGGHKAQGRTLE
                     SARALVADALAVRESGAQLILLEAVPPEVGGYITRTLDMPVLSIGAGGECDGQLLIVS
                     DMIGQFQAFTPKFVKKYCDVAGIVTDAMREYAADVRAGRFPAEENCYRMLEGEKERFD
                     AEFE"
     gene            complement(3270..3695)
                     /locus_tag="VFD74_09760"
     CDS             complement(3270..3695)
                     /locus_tag="VFD74_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZK49867.1"
                     /translation="MTSNRGRPPGEVLVDTSALYALLCSTDDCHAAARDAAEALLAHD
                     SALVTSSYVVAETAALLQSRLGRDAARGFLLDLVPYLDVAWVDEGLHARGLEAWLAQR
                     REGVSLVDCVSFALGHELRLEHAFTFDRRFVEQGFVVIP"
     gene            complement(3676..3951)
                     /locus_tag="VFD74_09765"
     CDS             complement(3676..3951)
                     /locus_tag="VFD74_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZK49868.1"
                     /translation="MIRMQIQFTDEQARALRRAAQQQGVSQSEVVRLSVDTFLGARSG
                     PDRTVLKARLQALVGAFKGADPDVAARHDDYLAHSIADWEPDDQQPR"
     gene            4171..4446
                     /locus_tag="VFD74_09770"
     CDS             4171..4446
                     /locus_tag="VFD74_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZK49869.1"
                     /translation="MGRQYSIAEARNHLSEAVHQAEVEGPITLTRRGRSVAVLIAEGD
                     YRRLQAARPGFWDAAAAFRGSVDVADLAGPEVYENIRDRSPGRGLGA"
     gene            4454..4870
                     /locus_tag="VFD74_09775"
     CDS             4454..4870
                     /locus_tag="VFD74_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZK49870.1"
                     /translation="MVYLLDTNIVSEALAVTPNAGVVRKLARHQRESVIAAPVWHELL
                     YGCLRLPASSRRRRILRFLEEVVGPSLDIVPYDGRVATLHATERARLEGLGRTVPFVD
                     GQIAATAVAHALILVTRNAHDFELFEGLRVENWFED"
BASE COUNT          798 a         1653 c         1722 g          836 t
ORIGIN      
        1 agagacgccc gcacgctgga gacccgccgt gtggatcgcg gggccaggat tccgccccga
       61 gggacccccc gtggggatcg cgccgacggg gtcctgacgg cggcatctcc gcggctggac
      121 cccgtcggcg cgatccccac ggggggtccc tcggggcgga atcctggccc cgcgatccac
      181 acggcgggtc tccagcgtgc gggcgtctct ctgctaccct gtccgaatga ctgaggcgcc
      241 cagacatgac gattcaaccc ggggcgatgc tccgcctgcg ggggccgctg cgcgcgacgg
      301 gggcagctcc ggagaagccg cggaccccgg cactcgcgtc ggggtcggcc gcacggtgcg
      361 ggccttcacc gacggggcct gtctcggcaa cccgggcccc ggcggatacg gcgccatcct
      421 catctaccga ggccgtcgca aagagttatc cggcggcgag cctgccacca ccaacaaccg
      481 catggagctg cgcgcgtgca tcgcggctct gcgggccctc aagcatccgt gcgacgtgga
      541 gctcacctcc gactcccgct acgtggtgca gggcatcgag gacggctggg ccgaacgctg
      601 gcggcgcaac ggctggatgc ggaccaagaa ggacccggcc aagaacgccg acctttggca
      661 ggagctgctg gacctctgcg ccatccaccg tgtgcgcttc ctctgggtgc gtggccacga
      721 cggtcaccct gagaacgagc gcgccgacga actcgcggtg gcggcggcgc ggcaggcggg
      781 ctgaggcagc ccgcctgccg gccggccagc tccgcgcgcc gtggcccggg atgaagaggc
      841 gcgcgaggtc gccgcgtccg acggccagcg ctcaggcgtg caggcgctcg gcgcacacga
      901 cgcccacacc cgcgcggatc agagcctccg ccgtgcggtt gacagcctcg tgtttgtcca
      961 agtagttgcg ctccagggcg ggcatcaggt cctgctccat gacggccgcc ttggtggcga
     1021 agtagtccag gatgtcgctc ggatgcgcgc ggcccgcctc gacctcgggg tctccgcctt
     1081 cgtgcttcgc cgagatgttg gggacctccc gcgctatctc caccagctcg gcgcggcgca
     1141 gatagggttg gatgacgatg ctgcggtacg cctcggtcac gtggtgctca aaacgcactg
     1201 ccttctccag accgagggta cgccggagat aggacgcctg gcggatggtc tcgttgtcga
     1261 ccgagttcga caggttgaaa cccacgagcg gcgtggaacc gtcgtcccgg gagaagaggc
     1321 gggcggtgag tagcgtccag aggatcgacc aggggttgtc gttgcccatg aacactgaga
     1381 tcttgaactc cacgtctacg cccagtttat ggagtaggta ggcagccatg acggtccccg
     1441 ggttaacgtt cagcacctgg cggacgccgt aggtggtgaa gtagcgcaga tactcgtcga
     1501 cccaggcgat cgcgtgctcg tttggttgac ccacaccgcc gaagtatccg gtgattgtgt
     1561 cggggccgcc caggtggacg ttcgagccgt ccgtgccctt ggtgtccagg gtctcgacgt
     1621 aggaggctcc cacgatttgc atggcggccg ccgtggcgag gatgtcgccc tggtcggcgg
     1681 tctgctcggc catgttgcgc acgcggatga agcggccggg catgagttcg cgccgctcaa
     1741 tggcctgccg ggcctcggcg atgagccaag ggaagtactg catggcgctg atttcaaggg
     1801 tcacggcgtt cgcgtcgtcg acgtcttccg ggcccgcttg gccggccagt gtccgggacc
     1861 ggtactcggc caaggagacg aaggctccgc tgtccctgcg ttcggtcagc cactcgaggt
     1921 gggcgaggta gggtgagtgt tcggcctcca gccgggccag cagattgtcc aggcgcccgg
     1981 cctcccccgc cttgcggttg atctcctccg ggccgccgtg cttctcgacg atttcgagca
     2041 gtgcgctcac ggtggggttg gcgggatcgg tgagtagggc gttgacttcg tccaagcgtc
     2101 caggcgcgat tctcaacgcc tcgcggtcaa gctgcatggt cgtgctccct tcgcggtagt
     2161 ggtcattccc cgcctgcaac cggccacacc gggcgaccgg tcatcctgct gcgggtcacc
     2221 atccccctgc gggtcacccc tattcgaact cggcgtcgaa cctctctttc tcgccctcga
     2281 gcatgcggta gcagttctcc tccgccggga aacggcccgc cctgacgtcg gctgcgtact
     2341 cacgcatggc gtccgtcacg atcccggcca catcgcaata cttcttaacg aacttggggg
     2401 tgaaggcttg gaattgcccg atcatgtcgg agacgataag cagctgtccg tcgcactcgc
     2461 cgccggcccc gatggacagg accggcatgt cgagcgtgcg ggttatgtag ccgcccacct
     2521 cgggaggtac cgcttccagc aggatcagct gagccccgga ctcccgcact gcgagggcgt
     2581 cggcgaccag cgcccgcgcc gactccagcg tccgcccctg ggctttgtgc ccgccgagtt
     2641 gcccggagct ctggggagtg aggccgatgt gtcccatcac gacgatgccc gcatcgacga
     2701 tggcccggat ctggggtgcc acgcgaacac cgccctccaa cttgaccgcg tctacgccgg
     2761 cttccttgag gaagcgcccc gcgttctcga ccgccttctc cgtcgagctc tgatagctca
     2821 tgaaaggcat gtcgccgatc acgaaggtgt tgggcgcgcc acgccggacg gcgtcggcgt
     2881 gcacgatgca ctggtccatg gtcacgggga tggtgcccga gtagccgtac acgcacatgc
     2941 cgaggctgtc gcccaccagc agcatatcga gaccggccgc ctcggcgaac tgggcggtgg
     3001 ggaagtcgta ggcggtgagg aaagtggcct tctcgccggt ggccttcatc gcgtagaact
     3061 cgtggatcgt cttcttggcc atcggctgtc tcctatctcg ctttcatgcc cgcagttcga
     3121 cgattccggg ggcccgagcg gggaagccgt ccctcggggt tggacaactc gaacctatca
     3181 cggatgcggg tcggggcgga ggacgtcatc gaggccctgc gcgctctggg gcacctgtgc
     3241 gacgtgcagc tcacctccgg ctacccggct cacgggatca cgacgaaccc ctgttcgacg
     3301 aaacggcggt cgaacgtgaa cgcgtgctcg aggcgcaact cgtggcccag ggcgaagctc
     3361 acgcagtcga ccagactcac accctccctg cgttgtgcca accaggcctc gaggccgcgg
     3421 gcgtgcagtc cctcatccac ccaggccacg tccaagtagg ggaccaggtc gagcaggaac
     3481 ccccgggcgg cgtcgcggcc gagtcttgac tgcaggagcg ccgcggtctc cgctaccacg
     3541 tagctactgg tgacgagagc ggaatcgtgg gctaggaggg cctcggcggc atccctggcc
     3601 gcggcgtgac agtcgtcggt cgaacacagc agggcgtaca gcgcggaagt gtccacgagg
     3661 acctcgccgg gaggtctacc gcggttgctg gtcatcgggc tcccagtcgg cgatcgaatg
     3721 tgccaagtag tcgtcgtgac gcgcggcgac atccgggtcg gcgcccttga aggcgcccac
     3781 gagagcttgg aggcgcgcct tcaagacggt gcggtctggc cccgaccggg ctccgaggaa
     3841 ggtgtccacc gaaagtcgga ccacttccga ctgggagact ccttgttgct gggccgcccg
     3901 gcgtagggcc cgagcctgct cgtcggtgaa ctgtatctgc atgcggatca aggtgacctc
     3961 cgcagtgagt atgggatccg gcggttctcc gatcacgtcg gcaagaatga gtataccatc
     4021 atgatggtat actgaagctc aatcgagtcc atgcgcgctg cacatccggg gagaatccag
     4081 cggaactgag accaacgacc gattgcaccg ggactgcgcg ctcggtacaa tggctatcta
     4141 ggaagtacga gatagccatg tgaggtcaga atgggcagac agtactccat cgccgaggcg
     4201 cgcaaccatc tgtccgaagc cgtgcatcag gctgaggtgg aaggtcctat caccctcacg
     4261 cggcgtggtc ggtccgttgc ggtgctcata gccgagggcg actaccggcg gctgcaggct
     4321 gctcgtccgg gcttctggga cgccgccgcc gcctttcgag gatcagtcga cgtggcagac
     4381 ttggccggcc cggaggtcta cgagaacatc cgggaccgga gtccaggacg cggcctgggg
     4441 gcatagggcc cgcgtggtct accttctcga cacgaacatc gtctccgagg cccttgccgt
     4501 cacgcccaac gcgggagtcg tgcggaagtt agcgcgccac cagcgcgagt cggtgatcgc
     4561 ggcccccgtg tggcacgaac tgctctacgg ttgcttgaga ctcccggcgt cttcccgacg
     4621 gcgacggatc ctgcgcttcc tcgaagaggt ggtaggtcca agtctggata tcgtgccgta
     4681 cgatgggagg gttgccacgc ttcacgccac ggagcgtgcc cgactcgagg gactggggag
     4741 gacggtgccg ttcgtcgacg ggcagatcgc cgccacagcg gtggcccacg cactcatctt
     4801 ggtcacccgc aacgcgcacg acttcgaact gttcgaagga ctccgtgtgg agaactggtt
     4861 cgaggactag cgcggcgtct ggatcgaggc cgaatggcgg atggactcgc gccacggccc
     4921 aggccgaacg gcgggcgact gcgcgccggc cgagggcaaa cggagtcgcg ccacggccca
     4981 ggccgaacgg cgggcggctg cgcgccggc
//
LOCUS       DASRMJ010000353         6945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_178978, whole genome shotgun sequence.
ACCESSION   DASRMJ010000353 DASRMJ010000000
VERSION     DASRMJ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178978"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFD74_09780"
     CDS             <1..633
                     /locus_tag="VFD74_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK49871.1"
                     /translation="SPDGTWVVTTESGVALQADAVILATPAFASSALVAGQSPELSRE
                     LSQIPYVTSATATLAYARSEFPAAPDGFGVVIPKPEHRRIKAFTWVTTKFFGRAPADT
                     VLVRCFVRPDDDSGAPLDDTALASAAQDEIADILGVRSSPLWTRVFRWERAMPQYVVG
                     HIERVERIFRLVEELPGLGLAGGAYRGSGIPDTVSHSRAEARRIVEGLAD"
     gene            complement(726..3176)
                     /locus_tag="VFD74_09785"
     CDS             complement(726..3176)
                     /locus_tag="VFD74_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK49872.1"
                     /translation="MAKRSSDLKEAKLPAGLTASQTYRRCDPASFAFATTAELPPLDE
                     VIGQARAVGAVDFGIGIHSQGYNIFALGPPGTGKASTISQFLERESAELPVPDDWVYV
                     NNFSLPHQPNAIRLPAGRGCEFRRDMEKLVENLQGAITTAFESEEYDARRREVSQSVS
                     ARQEKSIDSLRENAEAHGFVMVRTPAGLAFAPRTPEGETMSRDRYNELPAEEQKTIDD
                     ALEALNSDLQQVMRLVRQEEKGGRESLRELDEQVTTYAAKHLIDDVLEKWAGVAEIGG
                     HLDAVLADVVENAADFKASDDETPPTFMGIALPMRQRAEAAFRKYKVNVLVDNSALTG
                     APVVRETNPNLQNLVGRIEHQAQFGALVTDFGMIKPGALHAANGGFLMLEARDLFLKP
                     YAWDALKRTMKTGMIRIEDVTQQLGIAATSTLEPEPIPLRAKVVIVGEPYIYYLLHEL
                     DPDFQELFKVKADFDIIEDRTDDAELLYARFIAHICDQDGLPHFTADGVARVVEQASR
                     LVEDQQKLSTRFMSVMDLVRESAYWASLRRKRDPVVTAGDVQKAIDQRIHRSSRIEER
                     VREMIVDGTVMVDSRGEVVGQINGLSVSSMGDYAFGRPSRITATVRLGSGDVVDIERE
                     VDMGGPIHSKGVLILAGFLGARYAANRPLSLSARLVFEQSYSGVDGDSASSAELYAIL
                     SALSGLPIRQRFAVTGSVNQRGQVQPIGGANEKIEGFFRICRERGLTGDQGVLIPATN
                     VRHLMLASEVRAAIDAGRFHIYPVSTIDQGIEILTGVQAGRARAGVYPPGTVNRLVYE
                     RLSHLAERAREHGTPKEE"
     gene            complement(3239..4441)
                     /gene="kbl"
                     /locus_tag="VFD74_09790"
     CDS             complement(3239..4441)
                     /gene="kbl"
                     /locus_tag="VFD74_09790"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZK49873.1"
                     /translation="MDDATRELLAAQADDIRQAGLWKSERLLTSRQGALVTVGEGAQR
                     REVLNFCANNYLGLSSDPRVVAAAHAGLDERGFGMSSVRFICGTQDRHKELEGSLARF
                     FGFEDCVLYGSCYDANGGLFETLLTAEDAVISDALNHASIIDGVRLCKAQRYRYASGD
                     MTELEARLREASGARIRLIATDGVFSMDGTLAPLDRICDLAARYGALVMVDDSHASGF
                     VGPTGRGTPEHFGVQGRVDILTSTLGKALGGASGGFTAASTVVTDLLRQRSRPYLFSN
                     SLAPSIVAAGLAALRILSTMLELRERLMTNARRFREGMTEVGFTIKPGDHPIVPVMLG
                     DARLASAMADDLLVDGIYVIGFSYPVVPRGEARIRVQLSAAHEPDQVERAIAAFAEVG
                     ARHGALDR"
     gene            complement(4493..5662)
                     /locus_tag="VFD74_09795"
     CDS             complement(4493..5662)
                     /locus_tag="VFD74_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZK49874.1"
                     /translation="MTDTMLALTYDRVLDPWDSTTGLRMQRVARPVLDLDKDYRDGEA
                     ALIRVLLTGFCGSDRGIWYRRAFGDMISSSLAAEGRDVRIIGHELLGEVIQVGPEATR
                     DFGLRPGDIVTTESHIVDDTCYQCRIGDKHVCASAQIIGISLDGCFAEQIKLPAKALW
                     RTDLDRIRPEVAVLQEPFGNAVHACTKVNLRGQSVAIIGLGTIGLFAVAVARALGARR
                     VIGVEPVEANRAMGLALGADAVIRPSQPTPQSYIHDAAITQEIRHLTDGVGVDVAIEM
                     SGATAALNTAIAAARRGGNVILFGIRSGDAVIEHIDRVIVDGISLHSVVGRRIWETWH
                     ITRNLLEAQLSDIQQRVYDVILSGGRDCIVPVDEFSRETFGNLIDSHPKIILDWR"
     gene            complement(5779..5866)
                     /locus_tag="VFD74_09800"
     tRNA            complement(5779..5866)
                     /locus_tag="VFD74_09800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5830..5832),aa:Leu,seq:gag)
     gene            6000..6632
                     /gene="lexA"
                     /locus_tag="VFD74_09805"
     CDS             6000..6632
                     /gene="lexA"
                     /locus_tag="VFD74_09805"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZK49875.1"
                     /translation="MDLTSRQSEILRIIQETLGAQGYPPTVREIGMAVGLSSPASVQN
                     HLTALEEKGYIRRGASKRRAIEVVGVSSEWRGGRDARGRSEDALSWLPLVGRVAAGAP
                     LLADENVEESVQVPGFLANGGECFVLRVRGDSMVNAGILNGDLVVVRRQESAENGDIV
                     VALLGEEATLKRFFKENGHVRLQPENEAMKPIITRDPRIVGILTGVMRRV"
     gene            6632..>6945
                     /locus_tag="VFD74_09810"
     CDS             6632..>6945
                     /locus_tag="VFD74_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49876.1"
                     /translation="MSAAASAAFAGEGDDRAVAYPQDRQLRLVFEVGYAPPVGMSSGP
                     ADSLVAAGPISLAAYALSLRRGERCFCCGHPLETVESVGEGGGRRCLECPACGASLLG
                     PE"
BASE COUNT         1114 a         2199 c         2382 g         1250 t
ORIGIN      
        1 tctcccgacg gcacgtgggt ggtcaccacc gagtcagggg tcgccttgca ggcggatgcg
       61 gtgatcctgg ccacgcccgc cttcgcaagc agtgcactgg ttgccgggca gtcgccggag
      121 ttgtcgcgcg agttgtcgca gatcccgtat gtgacctcgg ccaccgcgac cctcgcatac
      181 gcgagatccg agttccccgc ggcgcccgac ggattcgggg tggtcatccc gaagccggaa
      241 cataggcgca tcaaggcctt cacgtgggtc accaccaagt tcttcgggcg ggctccggcg
      301 gatactgtgc tggtgcgctg tttcgtgcgg cccgacgacg acagcggtgc acctctggac
      361 gacacagcgt tggcctccgc cgctcaggac gagatcgccg acatccttgg cgtgcgaagt
      421 tctccgctct ggacgcgcgt cttcaggtgg gagcgcgcca tgccccagta tgtcgtgggg
      481 cacatcgaac gagtggaacg catcttccgg ttggtggagg agcttccggg cctcggtctg
      541 gcgggcgggg cgtatcgcgg atcggggatc cccgatacgg tcagtcacag tcgagcggag
      601 gcgcgccgca tcgtcgaggg tctcgcagac taggatcggg ccgcggtgtt cgacgcgtcc
      661 cggccagcgc gtcgctccgc gcgctccggt tggcgccgcg agcagacgtc gcctccgccg
      721 acgcgctact cctcctttgg ggtcccgtgt tcccgggctc tctccgccaa gtgtgaaagc
      781 ctctcataga ccagtcggtt gaccgtcccc ggaggataca cgcctgcccg cgctctgccg
      841 gcttgtactc cggtgaggat ctcgatcccc tggtcgatgg tggagacggg gtagatgtgg
      901 aacctgcccg cgtcgattgc cgcgcgcact tcggatgcga gcatgaggtg tcgtacgttc
      961 gtcgcgggga tcaacacgcc ctggtcgccg gtgagacctc gttctcggca gatccggaag
     1021 aagccctcga tcttctcgtt ggctccgccg atgggctgca cctgcccgcg ttggttcacc
     1081 gacccggtca cggcgaaccg ctggcggatg ggaagcccgg agagggccga caggatggcg
     1141 tagagctccg cggaggaagc gctgtcgccg tcgacgccgg agtatgactg ctcgaagacc
     1201 aaacgggccg acaggctgag cggacgattg gcggcgtagc gggcgcccag gaagccggcg
     1261 aggatgagca cccccttgga gtggatcggc ccgcccatgt cgacctcgcg ctcgatgtcg
     1321 acgacatcgc cgctgccaag gcgcaccgtg gcggtgatgc gactggggcg cccgaaagcg
     1381 tagtcgccca tcgacgacac cgagagtccg ttgatctgcc cgacgacttc gccccgactg
     1441 tccaccatga ccgtgccgtc gacgatcatc tcccgcaccc gctcttcgat cctggaactc
     1501 cggtggatgc gctggtcgat cgccttctgc acgtcgcccg cggtgaccac ggggtcacgt
     1561 ttgcggcgca gagacgccca atacgccgac tcgcgcacca agtccatcac cgacatgaag
     1621 cgggtggaga gcttctgttg gtcctcgacc agccgggacg cctgctccac gacccgcgcg
     1681 acgccgtccg cggtgaagtg cggaagtccg tcttggtcgc agatgtgggc gatgaaacgg
     1741 gcgtagagga gctccgcgtc gtcggtgcgg tcttcgatga tgtcgaagtc ggccttgacc
     1801 ttgaacagct cctggaaatc ggggtcgagc tcgtgcagca ggtagtagat gtacggttcg
     1861 cccacgatga cgaccttcgc ccgaagcggt atcggctccg gctccagcgt cgaggtagcc
     1921 gcgatcccca gctgctgggt gacgtcctcg atgcggatca tgccggtctt catggtgcgc
     1981 ttcagggcgt cccacgcgta gggcttgagg aagaggtcgc gggcctccag catgaggaag
     2041 ccgccgttag ccgcgtggag cgcgccgggc ttgatcatgc cgaaatccgt gacgagagcc
     2101 ccgaactgag cttgatgttc gatgcgaccg accaggttct ggagattcgg gttggtctct
     2161 cgcacgaccg gtgcgccggt gagggcggag ttgtccacca acacgttcac tttgtacttg
     2221 cggaaggccg cttcggcacg ttggcgcatg ggcagggcga tacccatgaa cgtgggggga
     2281 gtctcgtcgt ccgaagcctt gaaatccgct gcgttctcga cgacatccgc gagtacggcg
     2341 tcgaggtggc cgccgatctc ggcgacgccg gcccacttct cgagcacgtc gtcgatgagg
     2401 tgcttggcgg cgtaggtcgt cacctgttcg tccaactcgc gcagggactc ccgtccgccc
     2461 ttctcctcct gcctcaccag ccgcatcacc tgctgaaggt cggagttgag cgcctccagg
     2521 gcgtcgtcga tggtcttctg ctcttcggcg gggagttcgt tgtaccggtc tcgggacatg
     2581 gtctcgccct cgggggtgcg gggagcgaag gcgagaccgg cgggggtgcg taccatgacg
     2641 aaaccgtggg cctccgcgtt ctcgcgtagg gagtcgatgg acttctcttg gcgggcactc
     2701 acgctctgag acacttcgcg ccgtcgcgcg tcgtactctt cgctctcgaa cgccgtggtg
     2761 atggcgcctt gcagattctc aacgagcttt tccatgtcgc gccggaactc gcacccgcgg
     2821 cctgccggaa gacggatggc gttgggttgg tggggcaggc tgaagttgtt cacgtacacc
     2881 cagtcgtccg gtacaggcaa ctcagccgac tcccgctcca agaactggga gatggtgctc
     2941 gctttgcccg tgcccggagg tcccagcgcg aagatgttgt agccctgact gtggatgcct
     3001 atgccgaaat ccacggctcc gacggctctc gcctgcccga tcacctcgtc gagggggggc
     3061 agttcggcgg tggttgcgaa ggcgaaggaa gcgggatcac accggcggta ggtctgggag
     3121 gcggtgagcc ccgccggcag tttcgcttcc ttgaggtcgg agctgcgctt ggccatggtc
     3181 cctccgcact gtcggatctg gaacggtctg actctaccag gagtggcggc agatcggcct
     3241 accggtctaa ggctccgtga cgcgcgccca cctcggcgaa cgcggcgatc gcccgttcca
     3301 cctgatccgg ctcgtgcgca gccgatagtt gcacgcggat ccgtgcttct cctcggggca
     3361 ccaccgggta gctgaagccg atgacgtaga tgccgtcaac cagaagatca tcggccatgg
     3421 ccgaggcgag gcgggcgtcc cccagcatga ccgggacgat ggggtggtct cccggcttga
     3481 tggtgaagcc gacttccgtc atgccttcgc gaaaacgccg cgcgttcgtc atgagtcgct
     3541 cgcgcaactc caacatggtc gagaggatgc gcagcgcggc gaggcccgcc gcgacgatcg
     3601 agggcgcgag ggagttcgag aagagatacg gccgggatct ctggcggaga aggtcggtca
     3661 ccaccgtgga tgccgcggtg aagccgcccg aggcaccgcc gagcgccttg cccagggtgc
     3721 tggtgaggat gtcgacgcga ccctggacgc cgaagtgctc gggcgtgccc ctgcccgtcg
     3781 gtccgacgaa gccggacgcg tgcgaatcgt ccaccatcac gagcgcgccg taccgcgccg
     3841 ctaggtcgca gatgcgatcg aggggcgcca aggtgccgtc catgctgaag acgccgtcgg
     3901 tcgcgatcaa acggatgcgc gcgcccgagg cctcgcgcag ccgcgcttcg agctcggtca
     3961 tgtcgcccga ggcgtagcgg tagcgctggg ctttgcacag ccgaaccccg tcgatgatgg
     4021 aggcgtggtt gagcgcgtcg ctgatgacgg cgtcctcagc ggtgagcagc gtctcgaaga
     4081 gtccgccgtt ggcgtcgtag caggagccgt agaggacgca gtcctcgaat ccgaagaacc
     4141 gggcgagtga gccctccaac tccttgtgtc tgtcctgggt gccgcagatg aagcgcacgc
     4201 tggacatccc gaaccctctc tcgtccaggc ccgcgtgagc ggcggccaca acccgtgggt
     4261 cggaggagag gcccaggtag ttgttggcac agaagttcaa cacttcgcgg cgctgtgcgc
     4321 cttcccctac ggtgacgagc gccccctggc gggaagtcag cagccgctcg ctcttccaga
     4381 ggcctgcttg gcggatatcg tccgcctggg cggccagcag ttctcgtgtt gcgtcatcca
     4441 ccgatgcgcc tcctttcgcc gccgaacgct gcccttggtg tcgagccctg cgtcaacgcc
     4501 agtcgaggat gatcttgggg tggctgtcga tgaggttccc gaaagtctcc ctcgagaact
     4561 cgtccaccgg tacgatgcaa tcgcgcccac cgctcaggat cacgtcataa acgcgctgct
     4621 ggatgtcgga gagctgcgct tccaggaggt tgcgggtgat gtgccaggtc tcccagatcc
     4681 ggcgcccgac cacgctgtgc agggatatcc cgtccacgat gacccggtcg atgtgctcga
     4741 tgacagcatc tccgctgcgg ataccgaaca ggatcacgtt cccgccgcgc cgcgccgccg
     4801 cgatcgcggt gttgagcgcg gccgtggcgc cgctcatctc gatggcgacg tctacgccca
     4861 cgccgtcggt caagtggcgt atctcctgcg tgatggccgc gtcgtggatg tagctctggg
     4921 gcgtcggctg cgagggacgg atcacggcgt cggcgccaag cgccagaccc atcgccctat
     4981 tggcctccac gggttccacg ccgatgacgc gccgggcgcc gagcgctctg gcgacggcca
     5041 cagcgaacag gccgatggtg cccaggccta tgatggcgac gctctggccg cgcaggttga
     5101 ctttagtgca ggcgtgcacg gcgttcccaa agggctcctg gagcaccgcc acctccggac
     5161 gtatgcggtc gaggtcggtc ctccacaggg ccttcgccgg caacttgatc tgctcggcga
     5221 agcagccgtc gagcgagatg ccgataatct gggcgctcgc gcacacgtgc ttgtcgccga
     5281 tccggcactg atagcaggta tcatcgacga tgtggctctc ggtggtgacg atgtccccgg
     5341 ggcgcaggcc gaagtcgcgg gtggcttccg gccccacctg gatgacctcc cccaggagtt
     5401 cgtggccgat gatccttacg tcgcggcctt cggcagcgag ggacgacgag atcatgtcgc
     5461 cgaaggcccg ccgataccag atcccacgat ccgatccgca gaagccggtc aggagcacac
     5521 ggatgagggc ggcttctccg tcgcgatagt ctttgtcgag gtcgaggaca gggcgggcga
     5581 cccgctgcat gcggagtccc gtcgtcgaat cccagggatc aaggacacgg tcgtaagtaa
     5641 gcgcgagcat ggtgtccgtc acggggttct cctcgactgt ctgtagcctc gccggcgagt
     5701 cacgccgcca cactaccccg aagagggcgt cgtcaaaagc gcgcgccgaa acgaagtgag
     5761 gaggatcgga aggcgagatg gtgtccgagg ggggacttga acccccatga gttattcact
     5821 cactaggccc tcaacctagc gcgtctgcca attccgccac tcggacacag ccgtgggaaa
     5881 agcacgggtg agtatagatg gtgcccagag agccgtcaac aaccggcact tggcatcgtc
     5941 cgagggcccg cgtatactcg gctacgaaca aatgttcaca cgacttggag gtagcccaca
     6001 tggacctcac ctcacgccaa agtgagatct tgcgcatcat ccaagagacc ctgggggcgc
     6061 agggttatcc acccactgtg cgcgagatag gcatggcggt cggtctctcg tcgccggcct
     6121 ccgttcagaa tcatctcacc gcgctcgagg agaaggggta catccgtcgg ggtgcctcga
     6181 agcgacgtgc gatcgaggtg gtgggggtgt cctccgaatg gagggggggt cgcgacgccc
     6241 gcggtcggtc cgaagacgcg ttgtcgtggc tgcccctggt gggtcgtgtc gcggccggag
     6301 cgcccttgtt ggccgacgag aacgtcgagg agagcgttca agtccccggc ttcctcgcca
     6361 atgggggtga gtgctttgtc cttcgcgttc gcggtgacag catggtcaac gccggcatcc
     6421 tgaacggtga cctggtcgtg gtccgccgtc aggagagcgc ggagaacggt gatatcgtcg
     6481 tcgccctgct gggagaggag gccactctca agcgtttctt caaggagaac ggccacgtga
     6541 ggctgcaacc ggagaacgag gcgatgaagc ccatcatcac ccgtgacccg cgtatcgtgg
     6601 ggatcctcac cggcgtgatg aggagggtgt gatgtcggcg gccgcgtccg cggcgttcgc
     6661 gggcgaagga gacgaccgcg cggtggcgta tccgcaggat cgtcagttgc gccttgtgtt
     6721 cgaggtcggg tatgctccgc cggtcggtat gtcctccggg ccggccgatt cgctcgtcgc
     6781 ggctggcccg atcagtctgg cggcatatgc gttgagcctg cggcgcggcg agcgttgctt
     6841 ctgttgtggg catcccctcg aaacggttga gtcggtggga gagggtgggg gccggcgttg
     6901 tctggaatgc ccggcctgcg gagcgtccct gctcggtccg gagta
//
LOCUS       DASRMJ010000354         5449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_283838, whole genome shotgun sequence.
ACCESSION   DASRMJ010000354 DASRMJ010000000
VERSION     DASRMJ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283838"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFD74_09815"
     CDS             <1..423
                     /locus_tag="VFD74_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49877.1"
                     /translation="SIYFLTAENSADGALTDDQKERRARVIEFEMKLGDLHGWMGEAV
                     GPEAAFEWTAFSVLIQPGSPNGSDPSGIEPTVVEWPLADLAALGEVHPQGRRAVITGS
                     DLATLMPLVRAANQLTVWKSGGAYYHLLLRPLLPDEVE"
     gene            569..1750
                     /locus_tag="VFD74_09820"
     CDS             569..1750
                     /locus_tag="VFD74_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZK49878.1"
                     /translation="MYPFQMTEGIRRDLARLDELHARLDAQPLMRVWLGRTRRELEAE
                     VVAASTSMEGIAVTVDEVRRVLAGDRPRSVSAENVGLVEGYRDALDFVLRRADDPHFL
                     WQSELILGIHYRVLAGDYSHGAGRFRAGQNRVVDSAAQRHVYLPPPPESVPELVVELA
                     DQAQERRDAAAPITAALVHLQLAGIHPFSDGNGRTARILSSLVMYRGGFRRPEFTSLE
                     EWWGRHREDYYAAFACLGLEWNPGADATPFVEAHVGAQRRQVEALSLRLSTDRHLWLV
                     LEDLVRHDLRGDPRMVHALYDVFLGREVTNRYYRDLADVSVPTATNDLVRLDAAGLLR
                     VLGAGRTRRYSAGPRLMTLVATTARLEESVVDPEQSLEAQRDAILVSLAERIRAGGAG
                     G"
     gene            1857..2012
                     /locus_tag="VFD74_09825"
     CDS             1857..2012
                     /locus_tag="VFD74_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49879.1"
                     /translation="MAFDLVKGDDHKPSSTEGATIYLSAMVGFICFFLDSEGNRIGIQ
                     QPAANKR"
     gene            2176..3420
                     /locus_tag="VFD74_09830"
     CDS             2176..3420
                     /locus_tag="VFD74_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK49880.1"
                     /translation="MAKTLRSLSHRNFRIFWTSQLVSLVGTWMADVALAWLVLDLTDS
                     PVMLGAAMTLRFGPTLVFSLFGGVLADRLPKVRILMTTQSVLMLQAFVMAALTSSGHA
                     TITLVYLLALVRGTAESLDIPTRQAFVIELVGPADVQNAVALNSSVFNAARIVGPAIG
                     GVLVATVGVAVCFWLNAVSFAPVLVALALLRSADLHPVARPEKAPLFRQLGEGFAYAA
                     RTPDVALILAMVAVIGIFGYNFTVMLPLLARYALESGPIGLGALTGAMGVGALTAGVF
                     VAARGRPTRRTVLLGLGGFSVALLLLGLAPGLWPAMALLVVVGFFGILFHTSANSRLQ
                     LIVPGQLRGRVLSMYALLFIGSTPLGSLLVGTLAEHQGIRVAIVELSVVCMVGAGLSL
                     WVAVRMRRGIANPLPDLGRSPD"
     gene            3518..3754
                     /locus_tag="VFD74_09835"
     CDS             3518..3754
                     /locus_tag="VFD74_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49881.1"
                     /translation="MTADVKNLETHHLSSFVEIDYESRPEFFRFDSGLIFQSRRFRAA
                     MKDYCLRQEFITPYTPEQSGMIERFFRSLKEERV"
     gene            complement(4071..4994)
                     /locus_tag="VFD74_09840"
     CDS             complement(4071..4994)
                     /locus_tag="VFD74_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZK49882.1"
                     /translation="MDRVLDTRVRTAAFNWLSDQMRLHGDVLPRELLAQGFVLDDVRV
                     PLVGPPGIFKPKMLANVPLSLTTIPDGPYDDDIGVDDGLIYYRYRGTDPNHRDNQGMR
                     EAMRLRLPLIYFHRVAPGRYYAAWPVYIVHDDPGRLTVTVAVDDREHVLFKPVRADDL
                     ALYEDVDRGRRSYITAEVKVRLHQRGFRERVLEAYRRQCAFCHLRHEELLDAAHIIPD
                     WQPLGDPIVRNGLSLCALHHGTFDRNIVGLRPDYVIEVRPDILRERDGPTLAHAIQGL
                     HGQSIILPRTIAHRPDPDRLALRYGQFRQAS"
BASE COUNT          866 a         1734 c         1815 g         1034 t
ORIGIN      
        1 tccatctact tcctcaccgc tgagaacagc gccgacggcg ccctcaccga cgatcagaaa
       61 gagcgccggg cgcgggttat cgagttcgag atgaagctcg gcgatctgca cgggtggatg
      121 ggtgaggctg tcggtccaga agcggcgttc gagtggacgg ccttctccgt cctgatccaa
      181 ccgggtagcc ccaatggctc cgatccctcc ggcatcgaac caacggtggt cgagtggccc
      241 ttggcggacc tggccgctct gggtgaggtg cacccgcaag ggcgacgcgc ggtcatcacg
      301 gggtccgatc tggccacctt gatgcccctc gtgcgggctg cgaaccagct cacggtgtgg
      361 aagagcggcg gcgcctacta ccacctcttg ttgcgcccct tgctgcccga cgaagtggag
      421 taggctgcac ggaggttcct cgagaagcac cgccggctca acccggtcgt cgcagaccgc
      481 tccggttcgc tgtactccac ccgaagaagg gatccgtgtc tccctggagt ataatctcct
      541 aaattcatag gagattagga gattcgtagt gtacccgttc cagatgaccg aaggcattcg
      601 ccgagacttg gcccgcctcg acgaactgca tgctcggctt gatgctcaac ctctcatgcg
      661 cgtgtggctg gggcggactc gccgggaact cgaggccgaa gttgtggccg cctctacgtc
      721 catggagggc atcgccgtga ccgtggatga agtgaggcgc gtcctggcgg gcgatcgtcc
      781 ccgctctgta tcggctgaga acgtcggtct ggtcgaaggt taccgagacg ccctggactt
      841 cgtgctgcgt cgtgctgacg atccccactt cctgtggcag tccgagctca tccttggcat
      901 ccattatcga gtcctcgccg gtgactacag ccacggcgcc ggtcggttca gggccggcca
      961 gaaccgtgtg gtcgactcgg cggctcagcg acatgtctat cttccgccgc ctccggagtc
     1021 ggttcctgaa ctcgtggtcg agttggcgga tcaagcccag gagcggcgcg atgctgccgc
     1081 tccgatcact gccgccctgg tccacctgca gctggccggg atccatccgt tctcggacgg
     1141 caacggtcgg acggcccgga tcctttcgtc cctggtcatg tatcgcggcg ggttcagacg
     1201 tcctgagttc acgagtctgg aggagtggtg ggggcgccac cgcgaagact actacgccgc
     1261 tttcgcctgt ctgggcttgg agtggaaccc tggagcggac gcgacgccat tcgtcgaggc
     1321 ccacgtgggc gcgcaaagac gtcaggtcga ggccctctcc ttgcgcctgt cgacggatcg
     1381 acatctctgg ctggtcctcg aggatctcgt gcgtcacgac ctccgcggtg acccccgcat
     1441 ggtccatgcg ttgtacgacg tcttcctggg ccgggaggtc accaacaggt actaccgtga
     1501 cctggccgac gtctctgtgc ccacggccac gaacgacctg gtccgtctgg atgccgccgg
     1561 cctgcttcgc gtcctgggag cggggaggac ccgtcggtat tcggccggcc cgcgcctcat
     1621 gacgctggtg gcgaccacag cccggcttga ggagtcagtc gtcgatcccg agcagtcctt
     1681 ggaggcccaa cgggacgcga tcctcgtgag tcttgccgag cgcatccggg ccggcggcgc
     1741 cggcgggtga acgacgttcc acgaccgagc atggaacggg gcggtgctgc agaccgcgct
     1801 ggtgcagccg tttcccgggc agacggtggc cgtcccgccg cctagacatg ggtccggtgg
     1861 ctttcgacct cgtcaagggc gacgaccaca agccgagctc gacggaaggc gccacgatct
     1921 acctgagcgc gatggtcggc ttcatctgtt tcttcctcga ctcggaaggt aaccgcatcg
     1981 gcatccagca accggcggcg aacaagcggt aggtcgggcg cccattacgg gggccgccgc
     2041 gattctgccg cagccgtctg cgggcggtgt ggtcgtgacg cgaaggagta tagttcggcc
     2101 gccatgacga gtaccttgcc accgccactc acgccactca cgccacaccg ttccggcgcc
     2161 cgcgcgcggc ggggcgtggc taagacgctt cgctcgctca gccaccgtaa cttccggatc
     2221 ttctggacca gccaactggt cagtctggtg ggcacctgga tggccgacgt ggcgttggcc
     2281 tggctcgtgc tcgacctcac ggattcgccc gtgatgctgg gcgcggccat gacgctgcgc
     2341 ttcggcccca ccctcgtctt ctcgctcttc ggcggggtgt tggccgaccg gctgcccaag
     2401 gtgcgcatcc tgatgaccac ccaatccgtg ttgatgctcc aagccttcgt gatggcggcg
     2461 ctcacgtcga gcggacacgc cacgatcacg ctggtctacc tgctggctct ggtgcgggga
     2521 acggccgagt cgctcgacat ccccacccgg caggcgttcg tcatcgagct tgtagggccg
     2581 gccgacgtgc agaacgcggt ggcgctcaac tccagcgtct tcaatgcggc ccgtatcgtg
     2641 gggccggcca tcggcggcgt gctggtggcg acggtgggcg tcgcggtctg cttctggctg
     2701 aacgcggtga gttttgcgcc cgtgttggtg gccctcgcac ttctccgttc ggccgacctg
     2761 caccccgtgg cgcgcccgga gaaggcgccg cttttccggc agttggggga gggtttcgcc
     2821 tatgcggcgc gcacgcccga cgtggcgctg atcctggcca tggtggccgt gatcggcatc
     2881 ttcggctaca acttcacggt gatgctcccc ctgctcgccc gctacgcact cgagtcggga
     2941 cccatcggtc tgggcgccct gaccggcgcg atgggggtgg gtgcgctgac cgcgggggtg
     3001 ttcgtggcgg cgcggggccg cccgacccgg cgcacggtgc tccttgggct gggcggcttc
     3061 agcgtggctc tgctgctgct cggtcttgct cccggactat ggccggcgat ggcgctactg
     3121 gtggtcgtgg gcttcttcgg catcctgttc cacacgagcg ccaacagccg tctccagctg
     3181 atcgttcccg gacagctgcg aggccgggtg ctctcgatgt acgctctgct cttcatcggt
     3241 tcgacccccc tcggcagtct gctggtgggg acgctggccg agcaccaggg cattcgggtc
     3301 gcgatcgtcg aactctcagt ggtgtgtatg gtcggggccg gcttgagcct ctgggtcgcg
     3361 gtgcggatgc ggcgagggat agcgaacccg ctgccggatt tgggacggtc gcctgactga
     3421 gcgccgggcg tagtcggttg acggacggtg accggtgtcg ccggaccagg cctattggga
     3481 ctctggaaac cacgcgctcg aggtcggcag aggagatgtg actgctgacg tcaagaatct
     3541 cgagacccac catctctcct cgttcgtcga gatcgactac gaatcccggc cggagttctt
     3601 ccgattcgac tctggcctca tcttccagag ccgtcggttc cgggccgcta tgaaggacta
     3661 ctgcctccgg caagagttca tcaccccgta caccccggag cagagcggga tgatcgagcg
     3721 cttcttccgg agcttgaagg aagagcgggt ctgatagcac atgttcagtg acttcgcaga
     3781 agcccgtctg gcgatccgcg actggatcca ctagtacaac gagggacgac ctcatccgag
     3841 tctcggccac ctgagcccgg ccgaatatca ggcacagcag ttgcgactgg tggcttgaaa
     3901 tgggggggga gcattacact tgccgcctcg gtagccgtcg cggagcgggc agccgaagac
     3961 atccatgcgg acaacgccgt acctgcggca caggacggcg attgcgtcac gttacctttc
     4021 gagttctgcg agcatggtcg acctccatgc ctacggtacc ccgccggcag ctacgatgct
     4081 tgccggaact gaccgtaccg cagcgccagt cggtcgggat ccggacggtg ggcgatcgtt
     4141 ctgggcaaga tgatgctctg tccatgcagc ccttggatgg cgtgtgcaag cgtagggccg
     4201 tcgcgctccc gtaggatatc gggcctcacc tcgatcacgt agtcgggacg aaggccgacg
     4261 atgttccgat cgaacgtgcc atgatgaagc gcgcacaaag agaggccgtt gcgtacaatc
     4321 ggatccccca gcggttgcca gtcgggaata atgtgagcgg cgtccaagag ctcctcgtgg
     4381 cgaagatggc agaaggcgca ttgccgccgg taggcctcga gcacgcgttc tcggaaccca
     4441 cgctggtgga ggcgcacctt cacctcggcg gtgatgtagc tccggcggcc ccggtcgacg
     4501 tcttcataca gggctagatc gtcggcccgc acgggcttga agagaacgtg ctcccggtcg
     4561 tccactgcga cggtcacagt gagtcggcca gggtcgtcgt gcacaatgta cacgggccag
     4621 gcggcgtagt atctcccggg ggcaactctg tggaagtaga tgagcggcaa tctgaggcgc
     4681 atggcttcgc gcatgccctg gttgtcccga tggtttgggt cggtcccgcg gtagcggtag
     4741 tagatgagcc cgtcgtctac ccctatgtcg tcatcgtagg ggccgtcggg gatggtggtc
     4801 aacgagagcg ggacgttcgc cagcatcttc ggtttgaaga tgcccggtgg gccgaccaaa
     4861 gggacccgga catcgtccag aacgaagccc tgtgcgagga gctctcgtgg caggacgtcg
     4921 ccgtgtaagc gcatctggtc cgacagccag ttgaaggctg cggtacgcac gcgcgtgtcg
     4981 agaacacgat ccatggcacc cccgccttgt tcacagtcgc ccgaactcgc caacgtccca
     5041 gatcagcata ttcgtatcac gaacatccac aactccgaaa ggcggggtcg ccttcgtctt
     5101 tcgagaggac gggcactgcg gggggcgagc ggcgcagttc cgcgcgtacg gatagctccc
     5161 gtgtccggga cgcgatctac caggctggag tcggggttgc cccgcctgcg atctcagatc
     5221 cccgttggca acgtagctgt cgatctgccg ctatccggca ggaggctgtc ttcttcgcgg
     5281 gaacgaccgc ggtgccgcag acccttcctg ggtcgaaggt ccgatggtgc cggttcgacg
     5341 ggtatcccac acttggtgac aaagaacctc ccgaacacga atgatagaat acgaatacgt
     5401 atttgtacta cgctgaccga cggcaggcat gccgcgggcg ggcgattcg
//
LOCUS       DASRMJ010000355         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_320712, whole genome shotgun sequence.
ACCESSION   DASRMJ010000355 DASRMJ010000000
VERSION     DASRMJ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320712"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /gene="glgB"
                     /locus_tag="VFD74_09845"
     CDS             <1..1074
                     /gene="glgB"
                     /locus_tag="VFD74_09845"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HZK49883.1"
                     /translation="DWKSLIFNYGRHEVRSFLLSSALFWLDHYHADGLRVDAVASMLY
                     LDYSRKAGEWIPNIYGGRENLGAIEFLRRLNTEVYGHYPDVQCIAEESTAWPMVSRPI
                     YVGGLGFGLKWDMGWMHDTLEYMSYEPVHRKFHHDRLTFRALYAFTENFVLPLSHDEV
                     VHGKGSLLNKMPGDPWQKFANLRLLLSYMYGQPGKKLLFQGAEIGQWREWAHEASVDW
                     HLLGDERHAGIQRLLGDLNRLYREEPALHDLDLDEAGFEWIAANDSEQSVISFLRKAK
                     DGEVVLVALNFTPLPRHNYRLGAPSAGLWEEVLNSDAADYGGSGQGNLGGVQAAPFPF
                     HGRPASLTLTLPPLGAVFLKCRG"
     gene            1062..>2813
                     /gene="treZ"
                     /locus_tag="VFD74_09850"
     CDS             1062..>2813
                     /gene="treZ"
                     /locus_tag="VFD74_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HZK49884.1"
                     /translation="MPGLRVASRGWQPSLGAVPQGADRCLFRVWAPRADRVEVHLLGD
                     RERRVVLEPEPRGYHAALAEEVKPGTLYRYRLAGVAGTPGDEELPDPASRSQPQGVHG
                     PSQVVATETPPSAAFPGRPLEELVFYELHVGTFTPAGTFAAAIDHLDDLVDLGVTAVE
                     LMPVAQFPGQRNWGYDGAFPFAPHNTYGGPAGLRSLVSACHQRGLALILDVVYNHFGP
                     EGSRVADYGPYFTDVYRTPWGRAINLDGPDSDEVRRYFIENALHWVAEYGIDGLRLDA
                     LHALIDRSALPFLRELADAVHGYARATGRHIHLIAESDANDAALLAPPDRGGMGLDAH
                     WNDDLHHALHAVLTGERAGYYESFGGLEHLARALADGYVYGARWSPYRRRRHGSSPAA
                     IPAQRLLAYSQNHDQVGNRPLGGRLSSLVPFEALKLAAATVILSPYLPLLFMGEEYGE
                     TAPFLYFIDHGDPALVEAVRRGRSEEFSDFPGFTPDRPMPDPQDPLTLENSRLDRDLL
                     REPRHRALREFYRELLVLRCTDPALAHLSKETQTVETVPEETLVLRRRAATDEAESAA
                     TDRPAPSPRHEVSLVLHF"
BASE COUNT          443 a         1017 c          907 g          446 t
ORIGIN      
        1 gactggaaga gcctcatctt caactacggg cggcatgaag tgcgcagctt cctgctgagc
       61 agcgctctgt tctggctcga ccactaccac gccgacggcc tgcgggtgga cgcggtcgcc
      121 tccatgctct acctggacta ctcccgcaaa gcgggggagt ggatccccaa catctacggg
      181 ggacgcgaga atctgggggc catcgaattc ctgcgtcgcc tgaacaccga ggtctacggc
      241 cactaccccg acgtgcagtg catcgcggag gaatccaccg cctggcccat ggtctcgcgc
      301 cccatctacg tgggtggact ggggttcggt ctcaagtggg acatgggctg gatgcacgac
      361 actctcgagt acatgagcta cgagcctgtc caccgtaagt tccaccacga ccgcctcacc
      421 ttccgcgcct tgtacgcctt caccgagaac ttcgtgttgc ccctctcgca cgacgaagtg
      481 gtgcacggaa agggttcgct cctaaacaag atgccgggcg acccgtggca gaagttcgcc
      541 aacctccgac tcttgttgag ctacatgtac ggccagcctg gtaagaaact gctcttccag
      601 ggcgcggaga tcggccagtg gcgggagtgg gcccacgagg cgagcgtcga ctggcacctg
      661 ttgggcgacg aacgccacgc cggcatccag cgcctcctag gcgacctcaa ccgactctac
      721 cgggaggagc ccgccctgca cgacctcgac ctggacgagg cgggcttcga gtggatcgcc
      781 gccaacgact ccgagcagag cgtgatcagc ttcctgcgca aggccaagga cggcgaggtg
      841 gtgctcgtgg ccctgaactt caccccgttg ccccgccaca actatcgcct gggagcccca
      901 agcgccggcc tctgggagga ggtgctgaac agcgacgccg ccgactacgg cgggagcggg
      961 cagggcaacc tagggggagt gcaggcggct cccttcccgt tccacggacg gcccgcttcg
     1021 ctgacgctga ccctacctcc cttgggggcg gtgttcctga agtgccgggg ttgagggtgg
     1081 cgtcgcgggg ctggcagccg agcctcggcg ccgtgcctca gggcgccgac cgctgtctct
     1141 tccgggtgtg ggcgcctcgc gcggaccggg tcgaggtgca ccttctgggt gatcgtgaac
     1201 ggcgggtggt cctggagccc gagccgcgcg gctaccatgc ggccctggcc gaggaggtga
     1261 agccgggcac actctaccgt taccggcttg ccggtgtcgc cggcactccg ggagatgagg
     1321 agctgcccga cccggcctcc cgctcccaac cccaaggcgt gcacgggccc tcgcaggtgg
     1381 tcgcgacaga gacgcccccg tccgccgctt tcccggggag gcccctggag gagctggtct
     1441 tctacgaact gcacgtgggc acgttcacgc ctgccggcac cttcgcagcg gccatcgacc
     1501 acctggacga cctggtcgac ctgggcgtga ccgccgtgga gctcatgccg gtggcccagt
     1561 tcccgggaca acgcaactgg ggctatgacg gcgccttccc cttcgccccg cacaacacct
     1621 acggcggccc cgccggtctg cgcagcctgg tgagcgcctg tcaccagcgc ggactcgccc
     1681 tcatcctcga tgtggtctac aaccacttcg gaccggaggg ctcccgggtg gccgattacg
     1741 ggccctactt cacggatgtc taccgcactc cttggggccg ggctatcaac ctggacggtc
     1801 ccgacagcga cgaggtgcgc cgctacttca tcgagaacgc gctgcactgg gtggcggagt
     1861 acggcatcga cggtctgcgg ctggacgccc tgcacgccct catcgaccgc tcggccctgc
     1921 ccttcctgcg tgagttggcc gacgcggtcc acggctacgc ccgggccacg ggccgccaca
     1981 tccacctgat cgcagagagc gatgccaacg acgccgccct cttggcccca cccgaccggg
     2041 gcggcatggg tctggacgcc cactggaacg acgacctgca ccacgccctg cacgccgtcc
     2101 tgaccgggga gcgggccggt tactacgaga gcttcggcgg gttggagcac ctcgcccgag
     2161 cgctcgcaga cggctacgtg tacggcgccc gctggtcgcc gtacaggagg cggcgccacg
     2221 gcagttcgcc cgcggccatc cccgcgcaac gcctgctggc ctacagccag aaccacgacc
     2281 aggtgggcaa ccggcctctc ggcgggcgcc tcagtagcct cgtgcctttc gaagcgctga
     2341 agttggccgc ggccaccgtc atcctctcgc catatctgcc gctgctcttc atgggcgaag
     2401 agtacgggga gaccgcgcct ttcctctact tcatcgacca cggcgacccc gccctggtgg
     2461 aggccgtgag gcgcggccga agtgaggagt tctccgactt ccccggcttc acacccgacc
     2521 ggccgatgcc cgatccccag gatcccctca ctcttgagaa ctcccggctc gatcgggacc
     2581 tgctacgcga acctcggcac cgggccctgc gcgagttcta ccgtgagctc cttgtactgc
     2641 ggtgcaccga cccggccctg gcccatctga gcaaggagac acagacggtg gagaccgtgc
     2701 ccgaagagac tctcgtgctc cgacgccggg cggcgacgga cgaggccgag tcggcggcga
     2761 ccgaccgccc cgccccatcc ccgcgccacg aggtgtccct cgtgctccac ttc
//
LOCUS       DASRMJ010000356         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_214080, whole genome shotgun sequence.
ACCESSION   DASRMJ010000356 DASRMJ010000000
VERSION     DASRMJ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214080"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..374)
                     /locus_tag="VFD74_09855"
     CDS             complement(3..374)
                     /locus_tag="VFD74_09855"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZK49885.1"
                     /translation="MMREPWVQVRLSELAEEHDESYGYYDLSKALDLNKLRANIFRFA
                     PGEKMEYHSHTEQEEFFFVLEGQCTLVVDGRRVPLAQGSLIRLDPRPRRQIVNDSDAD
                     CLWLGVGAPGIDNEWVEHREE"
     gene            complement(560..2176)
                     /locus_tag="VFD74_09860"
     CDS             complement(560..2176)
                     /locus_tag="VFD74_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZK49886.1"
                     /translation="MESDTPAALRALYDQVAKCGKCGFCQPACPVYRTTYREAHVARG
                     KHALVRNLIEGETVLDADLRDAFDNCLLCRACTASCFSDLKTDRLVVAFREAYARRFG
                     RSTLQRVIFRGLLPDPRRMRQVVWAAWAAREAGLDGVAARWGLLDMINPKLTKALELR
                     EGAPGQFLRVRLARPAAEAGGRRGGLTAPILPPTRDAGAESWERGDRPVRVGYWISCG
                     YNYMLPEVGVAAVSVLRRAGASVEVLPNACCGLPVYGYGDVDGARALARRNIEVMGDL
                     TRFDAVVSECGSCSGHLKEYPELFADDGEFRTRARRLAAKVRSFSEFLVEQGCTEIPA
                     ARATTRSQQPQSAQRAQSMPLGPSPRPPQIAPFGPSLRLPQAPEPSLQPRALHVERPG
                     LGAGGPAWESRRVGSPPLSPTLDAGGPGEQPGRPLVVTFHEPCHLGARFQGVVSQPRD
                     LLRSLPGVEFRELPEADRCCGAAGSYNVLNPEVSAAILARKMEKVASTGADVVVTECP
                     SCMLQLSLGAKRAGSKVRVLGISEMLAGLY"
     gene            complement(2166..3599)
                     /locus_tag="VFD74_09865"
     CDS             complement(2166..3599)
                     /locus_tag="VFD74_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZK49887.1"
                     /translation="MSSRMLSDEVVAHLQGLVGSRNIRESPDHHVAGVRPCAEVLVGD
                     VDELADVMRVAAESDLTVCPTGGGTKLGWGAPPRACDLLVRTTAWRAEGSVGSGEARH
                     AEGHLLPPLGSMGAITEYDPDNLTVTAGAGTTLAALQDLAARDGLLLPLDPPMPESAT
                     LGGVLSTNDHGPHRMLHGSMRDVVLGLGAVLVGGDRVKAGGRTIKNVTGYDLTRLFIG
                     AFGSLGVMSEATIRLLPGPSREHVVVGSMPDLAAADAAVRGLLGSQLIPAAVEVLSPA
                     SSRMVDADVVGIMGAGGHVVLVAFEGDAVAVERQVRDVVRVLGEEGTTLILTAPGPVS
                     SVWSALAAQRRAARSAGYSVEAKLSVPRSELLGLLGLAESLPAAAQGDLMWTASAGSG
                     TARLWLGSPGTVGAAPPGCCDLLLRVRAAAERVGGSLVLVEGADILCGELDVWGADPG
                     GVDLMRTLKGKFDPRGLMNPGRCAGGI"
     gene            complement(3596..>4052)
                     /locus_tag="VFD74_09870"
     CDS             complement(3596..>4052)
                     /locus_tag="VFD74_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZK49888.1"
                     /translation="KGRKGAFGAVSRLAPSYIVADGTVPRTRLPETIRAVTEIARRHD
                     LQVANVFHAGDGNLHPLFLFDDRVPGDRERVMAAGMEVLQLCADMGGTVSGEHGIGLE
                     KVAAMTMVFDESDMRAQEWVKDSFDPNDLCNPGKVLPPRGERRHEEEGR"
BASE COUNT          690 a         1515 c         1269 g          578 t
ORIGIN      
        1 tctcattcct cccgatgctc cacccactcg ttgtcgatgc ccggagcgcc cacgccgagc
       61 cagagacagt cggcgtccga atcgttcacg atctgacgtc tcgggcgcgg atccagccgg
      121 atcagggagc cctgtgcgag cggtacgcgc ctgccgtcca cgaccagcgt gcactgccct
      181 tcaaggacga agaagaactc ctcctgttcg gtgtgggagt gatactccat cttctccccg
      241 ggcgcgaacc ggaagatgtt ggcccggagc ttgttcaggt cgagcgcctt cgagaggtcg
      301 tagtagccgt acgactcatc gtgctcctcc gccagttcgg aaagcctcac ctggacccac
      361 ggttctcgca tcatcgtccc ccccccttga cattctcgcg ccttctcgga cacgcaccgg
      421 gccccgggta gacgatcggg ctgaagggtg acgatccaga gagcacttgc tgaagtccat
      481 cctatcgcgc ctcgaccgcg agggaaacca ggccggcgag ccgcaggcgc gctccgcgaa
      541 cggcccatac tggagcgtgc tagtagagtc cggcgagcat ctccgagatc ccgagcaccc
      601 gcaccttcga tccagcgcgc ttcgccccca acgaaagctg cagcatgcac gacgggcact
      661 cggtcacgac cacgtcggcg ccggtcgaag ccaccttctc catcttccgc gctaggatcg
      721 cggccgacac ctcggggttg aggacgttgt acgagccggc cgcgccgcag cagcggtcgg
      781 cctcggggag ttcacggaac tccacacccg gcagcgagcg cagcagatcg cgcggctggc
      841 tcaccacccc ctggaagcgg gcgccgaggt ggcacggctc gtggaacgtc accaccagcg
      901 gcctgcccgg ctgctcgcca ggaccgccgg cgtccagcgt aggcgacagc ggaggcgagc
      961 cgacgcgcct ggactcccac gcggggccgc cggcgccgag cccggggcgt tcgacgtgca
     1021 acgcccgggg ctgcagtgac ggctccggag cctgcggcag ccgcagtgac ggcccgaacg
     1081 gcgcaatctg cggcggccgc ggtgacgggc cgagcggcat gctctgcgcc cgctgtgcgc
     1141 tctgcggctg ctgcgatcga gtcgtggcgc gcgccgcagg aatctcggtg cagccctgct
     1201 cgacgaggaa ctcactgaaa gagcggacct tggccgccaa ccggcgcgcc cgcgtgcgga
     1261 actctccgtc gtcggcgaac aactccggat actccttcag gtgccctgag cagctgccac
     1321 actcggacac gacggcgtcg aaacgagtga ggtcacccat gacttcgatg ttgcgccggg
     1381 cgagagcccg cgcgccgtcc acatctccat agccgtacac cgggagtcca cagcaggcgt
     1441 tgggcaacac ctccaccgac gcccccgccc gacgcaacac cgacacggcc gctaccccca
     1501 cctcgggcaa catgtagttg tagccacagc tgatccagta cccgaccctg acgggtcggt
     1561 caccgcgctc ccaggactct gcccctgcgt cgcgagtcgg cggtaggatc ggagccgtga
     1621 gccccccgcg ccgacccccg gcctcggcgg ccggcctggc cagacgcaca cgcaggaact
     1681 gtccgggcgc tccctcccgc aactcgagcg ccttcgtgag cttgggattg atcatgtcca
     1741 gcaggcccca gcgtgccgcg acgccgtcaa gacctgcttc ccgagcggcc cacgccgccc
     1801 acacgacctg ccgcatgcgc cgcgggtccg gaagcaaccc acggaagatc accctttgca
     1861 gcgtcgaacg gccgaaccgg cgcgcgtatg cctcgcgaaa ggcgaccacg aggcgatcgg
     1921 ttttcagatc ggagaagcaa gaggccgtgc aggcccggca gagcaaacag ttgtcgaagg
     1981 cgtcccgcag gtccgcgtca agaacggtct ccccctcgat gagattgcgc acgagggcgt
     2041 gcttgccccg ggcgacatga gcctcgcgat aggtcgtgcg gtagacgggg caggcgggtt
     2101 ggcagaagcc acacttgccg cacttcgcca cctggtcgta gagcgccctc agagccgcgg
     2161 gggtatcaga ttccacccgc acaccttccg gggttcatca gaccccgggg atcgaacttc
     2221 cctttcaacg tcctcatgag gtccacccca ccgggatcgg caccccaaac gtccagttcg
     2281 ccacagagaa tgtcggcccc ctcgaccaag accagacttc ccccgacacg ctcggcagcc
     2341 gcgcgcacgc ggagcaggag gtcgcagcat cccgggggcg ccgcgcccac tgtcccaggc
     2401 gagccgagcc acagccgcgc cgtcccgctg ccggccgacg ccgtccacat caggtctcct
     2461 tgcgcggccg ccggcagaga ctccgcgagg ccgagtagcc caagcagttc cgatcgcggc
     2521 acgctcaact tcgcctccac cgagtaaccg gccgagcgcg cggctctcct ctgtgcggcg
     2581 agcgccgacc acacggacga gaccggccca ggcgccgtca gaatgagcgt cgtgccctcc
     2641 tcgcccaaca cgcgcacgac atctcgaacc tgacgctcca ccgcgaccgc gtccccttcg
     2701 aaagcgacca agacgacgtg gccgcccgcc cccatgatgc ccacgacgtc cgcgtcgacc
     2761 atccggctcg aggccgggga caggacctcg acggcggccg ggatcagctg ggaacccaac
     2821 aacccgcgca cggccgcatc cgcggcggcg agatcgggca tggaccccac taccacgtgc
     2881 tcgcgggagg ggccgggaag cagtcggatc gtcgcctcgg acatgactcc gagagagccg
     2941 aacgcaccta tgaagagacg ggtgaggtcg tatcctgtga cgttcttgat agtgcgacca
     3001 cccgccttca cgcggtcgcc ccccacgagc accgcgccca ggccgagcac gacgtcgcgc
     3061 atactcccat gcagcatccg gtgcggaccg tggtcgttgg tgctcaggac ccccccgaga
     3121 gtcgctgact ccggcatagg tggatcgagt ggcagcagca gcccatcacg cgcggcgaga
     3181 tcctgcagtg cggcgagggt ggtgcccgcc cccgccgtca cggtgaggtt gtcggggtcg
     3241 tactcggtga tggcccccat agacccgagc ggcggcagca agtgcccctc cgcatgccgg
     3301 gcctcccccg agccgaccga tccctccgcg cgccaagccg tggtgcgcac cagaaggtcg
     3361 cacgcacgcg gcggagcgcc ccagcccagc ttggtgcctc ccccggtggg acacacggtg
     3421 aggtcggact cggccgcgac tctcatgacg tccgcgagtt cgtccacatc gcctacgagt
     3481 acttcggcac agggtcggac ccccgccacg tggtgatcgg gcgactcgcg gatgttacgc
     3541 gaaccgacca gaccctgcag gtgagccacc acctcatctg agagcatgcg cgagctcatc
     3601 ggccctcctc ctcgtgccgt cgttccccgc gcgggggtag caccttcccc gggttacaga
     3661 gatcgttcgg gtcgaacgag tccttgaccc actcctgggc gcgcatgtca ctctcatcga
     3721 acaccatggt catcgccgcc accttctcca agccgatgcc gtgttccccg ctgaccgtcc
     3781 cacccatgtc cgcgcacagc tgcagcactt ccatgcctgc cgccatcaca cgttctcggt
     3841 ctcccggaac ccgatcgtcg aacaggaaca gcggatgcag gttgccgtca cccgcgtgga
     3901 acacgttggc cacctgcaga tcgtgccggc gtgctatctc ggtcacggcg cggatggtct
     3961 cgggaaggcg ggtgcgcggc acggtgccgt cggcgacgat gtagctcgga gccagccggg
     4021 agaccgcccc gaacgccccc ttgcgcccct tc
//
LOCUS       DASRMJ010000357         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_179353, whole genome shotgun sequence.
ACCESSION   DASRMJ010000357 DASRMJ010000000
VERSION     DASRMJ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179353"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(592..789)
                     /locus_tag="VFD74_09875"
     CDS             complement(592..789)
                     /locus_tag="VFD74_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49889.1"
                     /translation="MLVPVGLDGKRFEGGSDTMNGWNDMMNGWNGGAGGVLMWLLVAL
                     VVVAIIVGVVLLVRGTGRGKR"
     gene            909..1025
                     /locus_tag="VFD74_09880"
     CDS             909..1025
                     /locus_tag="VFD74_09880"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZK49890.1"
                     /translation="MVSRTHSGTTISGFIHILLVPAIIVVVVRLIQGRRAAR"
     gene            1324..2043
                     /locus_tag="VFD74_09885"
     CDS             1324..2043
                     /locus_tag="VFD74_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HZK49891.1"
                     /translation="MISAFLWGGFAASSLLLGYLLAERGLSNRTIGLIMGFGAGALIS
                     AIAYELVPESAIGGWEMPAAFALGAAVFFSVDWLVDRMGGVHRKSIAGKKGEGSGAAI
                     FIGTLLDAIPESLVLGIGVAIGGSIRVAFLAAVFVSNVPEGVAGTVNLSAAGHSRLRT
                     LWMWIALVLASAAAAALGYGLIQWIPAADGRVVQAFAAGAMLTMLADAMMPEAFEHGG
                     RLVGLFTVMGFAAAAALSILG"
     gene            complement(2000..2389)
                     /locus_tag="VFD74_09890"
     CDS             complement(2000..2389)
                     /locus_tag="VFD74_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49892.1"
                     /translation="MLLFLGAPNDWLISEIGSNSLDAVSGVQRVYTWALMFLALVGGF
                     AGLRKERQTLLPLLIFFYILGVVSLFYFQARYRLPAMPFVIIMAAYGVSVIGRLLPLP
                     SRPSVGGGARAIHIIRVSRELRRRRTP"
BASE COUNT          472 a          872 c          857 g          493 t
ORIGIN      
        1 catcgcgatc gacggggaag ccgctacggt cccgtcgccc atacgcttcg agagcctgcc
       61 gggggcactg cgcgtgcgcg tgccccggta ggcaaccggg ctttcggcgg tcgcgcgccg
      121 actcatcccg ggcggtcgtt cgatacatgg cttttgaacg tcctggaagg ttctcgagcg
      181 ctcccgttct tgaccaaccg aatgatcggg atgcacgtca agcggctcac cggtatgcag
      241 actgtgcgcc tcgccacctc gacgagaacg ggactcatca ccagctgcac caagctgcga
      301 taccacggtc cggctccagc cgacgaacgc cctgggaccg cagacctggc caggtggctc
      361 tctgaggcgt ggcaccacta tcagggcacc tgggccgccg tggaccaggt gccctacgat
      421 ctctgtcggc ggcggtggtg gcgccagcag aggttcgccc ggccgctgac gcgagcgtct
      481 tcctgaaggc cattcattcc cgagaagatt gcacgcgcct ttccgtgtcg agcgctctca
      541 cctggcggag acggtctgct tcacgtcaga agtgtcctgc aggtcgagcc atcatcgctt
      601 tccacggccg gtgccccgaa cgaggagcac taccccgacg atgatggcga ccacgaccag
      661 agctacgagc agccacatga gtactccgcc cgcacccccg ttccacccgt tcatcatgtc
      721 gttccaccca ttcatcgtgt cgctgccccc ctcgaatcgc ttgccgtcga gccctacagg
      781 tacgagcacg gtcacccctc cggtgggtcc ctaaaccccg tgcgtgaacg gacacggcat
      841 gcttttggtg tctcagggga ggaggagagt cgtatgccat ggacaatcgc agtggtgctc
      901 gtgattctat ggtcagccgg actcattccg gaacaaccat cagcgggttc atccacattc
      961 tcctcgtgcc ggccatcatc gtcgtagttg ttcggctgat tcagggtcgg cgcgcagctc
     1021 gttgaggagg tccggcgatc caaggcgcgg ccgaccgcct ccccgggagc atgcggcacc
     1081 cccaagtaca cgcgcatcga gaagaccttc tcctttgccc actgcaccgg cagtctccaa
     1141 ggttgacctc accggccgcc ctcagcgagc tgaacggcta cgctaaagcg ttaccgcgga
     1201 gcactcggtg gctgccgatg cgatagtgtg agccctgatg tgacgcgagg aatgctccat
     1261 ctcccctgag gaccgacagc ggcgactcgt agcggatgga gccggcaggg aaggcgactc
     1321 tccatgatct ctgcgtttct ttggggaggt tttgcggcaa gctcgttgct gctcgggtat
     1381 ctgcttgccg agcgcggact ctccaaccgg acaataggtt tgatcatggg gttcggcgcc
     1441 ggcgcgttga tcagcgccat cgcttacgag ctggtgcccg agtctgccat cggaggctgg
     1501 gagatgcccg cagccttcgc gctgggcgcc gcggtcttct tctcggtcga ctggctcgtc
     1561 gatcgcatgg gtggggtgca ccgcaagtcc atcgcgggca agaaggggga aggttcgggt
     1621 gcggccatct tcatcggcac actcctcgac gctatccccg agtcgcttgt gctggggatc
     1681 ggcgtcgcga taggcggctc gatacgtgtg gccttcctgg cggcggtctt cgtctcgaac
     1741 gtaccggaag gggtcgccgg gacggtgaac ctgtcagccg cgggccactc ccgactcaga
     1801 accctctgga tgtggatcgc gctggtcctt gcctcggccg ccgccgcggc gctgggttac
     1861 ggcctcatcc agtggatccc cgccgcagac gggcgcgtgg tgcaagcctt cgcggccggg
     1921 gccatgctca ccatgttggc cgatgcgatg atgccggaag cctttgagca cggcggcaga
     1981 ttggtggggc ttttcaccgt catggggttc gccgccgccg cagctctctc gatactcgga
     2041 tgatgtgaat ggctctggcg ccgccgccca cggacggtcg tgaggggagt ggcagcagtc
     2101 ggccgatcac gctcaccccg taggcggcca taatgatgac gaacggcatg gccggaagac
     2161 ggtatcgggc ctggaagtag aacagggaga cgacacctag gatgtagaag aagatcagca
     2221 gcggcaacag agtctggcgc tcttttctga gaccggcgaa tccacctacg agggcaagga
     2281 acatcagagc ccacgtgtag acccgctgga cgcccgagac cgcgtcgagg ctgttcgacc
     2341 cgatctcgga tatcaaccag tcgttgggag cgcccaagaa cagcagcatc cgccaacccg
     2401 tgagggccag gaaactagcg agtcaagctt gccacggccc tgcatccagt ggtacgctcc
     2461 ctcggatgct caagactcaa ccgacaccca gaaaccacat cggcgaacac tcgctttgtt
     2521 cgtggtggcg ctggcccgat acacggggct gagcgccggc acgttcctga cataggaaca
     2581 tagacgccgc gggcggccct cagggaccgt ccgcggcgtt tctcgttgta cgacagaaag
     2641 gccgcggagc actccgcggc ctttctgcat tctccgaccg ttcgaaaggg gagg
//
LOCUS       DASRMJ010000358         3886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_321214, whole genome shotgun sequence.
ACCESSION   DASRMJ010000358 DASRMJ010000000
VERSION     DASRMJ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321214"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFD74_09895"
     CDS             complement(<1..342)
                     /locus_tag="VFD74_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HZK49893.1"
                     /translation="MSRSSDILLYDTTLRDGMQREGLSVSVDEKIRIAQRLASVGIHV
                     IEGGFPGSNPKDVEFFSRIANVDLGDARVSAFGMTRARGVSAADDPVLRTLAESWAPI
                     SCVVGKTWALHI"
     gene            complement(339..1415)
                     /locus_tag="VFD74_09900"
     CDS             complement(339..1415)
                     /locus_tag="VFD74_09900"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZK49894.1"
                     /translation="MRYDIGVLPGDGTGPEVVREGLKVLRAAADRAGFSYDLHEYDFG
                     AERYLRTGETLPDSGLEELARHDAIFLGAIGHPDVKPGILEQGILLKARFALDLYINL
                     RPVKLYPGVETPLKDKGPADIDFVVVRENTEGLYASTGGFLKKGTPDEIAVQESVNTR
                     KGAERCIRYAFEYAGRPDRGAKLTLCGKTNVLTYAWDLWWRAFNEVGEADYPNITRDY
                     AHVDAIVMWFVKNPEWFDVIVTDNMFGDIITDLAAMIQGGLGIAAGGNINPEGTAMFE
                     PMGGSAPKYAGQNVINPLAAISSLQMMLDHLGQKDAAQWVEAGIIHAISRMDSLAAGK
                     MGMGTSDVGDIIARHVAEGTPSVE"
     gene            1566..2381
                     /locus_tag="VFD74_09905"
     CDS             1566..2381
                     /locus_tag="VFD74_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZK49895.1"
                     /translation="MDPHKTRPRAAASIAPALRVGEVARLAHVSVRTLHHYDHVGLLE
                     PSGHTASGYRLYTLPDLERLQHVLLYKELGFSLEEIRESLTDPSFGRREALQGQRGLL
                     EEQMLRLEALLGLIDKTLVSLEGGFGMTKEEMFEVFDGFDPAEYEDEVKERWGHTEAY
                     KESARRTKGYTKADWERFKAESEEIGTAIAALMDEGIPPEDPRAMDQVERHRLQIDRW
                     FYPCSHEMHVGLGQMYVADPRFTATYEKIHPGMAQYVCDAIIANAARAEAERR"
     gene            complement(2496..2915)
                     /locus_tag="VFD74_09910"
     CDS             complement(2496..2915)
                     /locus_tag="VFD74_09910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK49896.1"
                     /translation="MILPFDITDYDEVLSLWKRCEGIGLSGADERANILVYLERNPGS
                     SFVARADGVLVGAVLCGHDGRRGFIHHLAVDPTWRRRGIGTALVEACVSSLRSNGIQK
                     CHVSVYHENEGAVGWWRRRGWVLRTDIAVMSAEIESV"
     gene            complement(2945..3352)
                     /locus_tag="VFD74_09915"
     CDS             complement(2945..3352)
                     /locus_tag="VFD74_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49897.1"
                     /translation="MSSKRNLARRAISYQLIGFSILVTLIACDEVLDLPHKVLGAPAT
                     PINWTEAALESSFVLVLGAISIGLTWRLLDRLVMLEGLLPVCSFCKKIRKHDEWEVLE
                     KYIGEHSEAVFSHGVCPECAEKYYGEFVGDDSP"
     gene            complement(3495..>3886)
                     /locus_tag="VFD74_09920"
     CDS             complement(3495..>3886)
                     /locus_tag="VFD74_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49898.1"
                     /translation="LLSVLSVVSVLSVFSGCEAGSGAARISDAWMSADEEGAERVATY
                     AQDAIFYAHVELTDAPADTNLKASWVAVEVDDADPGLIIDEVETATSSGPTFFTLTNE
                     GPWPLGTYRVELYVGDELLKSVEFDVR"
BASE COUNT          701 a         1285 c         1232 g          668 t
ORIGIN      
        1 gatatggaga gcccaggtct tgcccaccac gcacgatatg ggcgcccagc tctcggccaa
       61 ggtgcgcagc acggggtcgt cggcggcgga cacaccccgg gcccgcgtca taccgaaagc
      121 actcacgcgg gcgtcaccca agtcgacgtt cgcgatgcgc gagaagaact ctacgtcctt
      181 gggattggac cccgggaagc cgccttctat gacgtggatg cccacagagg cgagtcgctg
      241 ggcaatccgg atcttctcgt ccactgacac gctcaggcct tcgcgctgca tcccatcgcg
      301 cagggtggtg tcgtacagga gtatgtcgga agaacggctc actcgaccga cggagtgcct
      361 tcggctacat ggcgggcgat gatgtcaccg acatcgctgg tgcccatgcc catcttgccg
      421 gcggccaacg agtccatgcg gctgatcgca tggatgatgc cggcttccac ccactgcgcc
      481 gcgtccttct gccccaggtg gtcgagcatc atctgcagcg aggagatcgc cgccagaggg
      541 ttgatcacgt tctgccccgc gtacttgggg gccgagccgc ccatgggctc gaacatggcc
      601 gtgccctcgg ggttgatgtt gccgcccgca gcgatgccga gacctccctg gatcatggcg
      661 gccaggtcgg tgatgatgtc gccgaacatg ttgtcggtga caatgacgtc gaaccactcc
      721 ggattcttca cgaaccacat gacgatggcg tccacgtggg cgtagtcgcg ggtgatgttc
      781 gggtagtcgg cctcgccgac ctcgttgaag gcgcgccacc agagatccca ggcgtaggtg
      841 agcacgttgg tcttgccaca gagggtcagc ttggcgccac ggtcgggccg gccggcgtat
      901 tcgaaggcgt agcggatgca gcgctcggcg cccttgcggg tgttcacgct ctcctgcacg
      961 gcgatctcgt cgggagtccc cttcttgagg aatcccccgg tggaggcgta caggccttcg
     1021 gtgttctcgc ggacgaccac gaagtcgatg tccgccggac ccttgtcttt gagcggcgtc
     1081 tccacgcctg ggtacagctt gacgggcctc aggttgatgt agaggtcgag ggcgaagcgc
     1141 gccttgagga ggatgccctg ctccagtatg cccggcttca cgtcggggtg gccgatggct
     1201 ccgaggaaga tggcgtcatg acgggccagt tcctcaagcc cggagtcggg gagagtctca
     1261 cccgtgcgca gataccgttc ggcgccgaag tcgtactcgt gcagatcgta cgagaacccg
     1321 gcgcgatcgg ccgccgcccg caggactttc aagccttccc gcaccacttc cggccccgtt
     1381 ccatcaccgg gtagcacgcc gatgtcgtag cgcattcctt ctccctctcc tcgacgaagg
     1441 cctatcatcc gggcctcccg ctcttctcgt ccgcacattg tatcttccgc aaggcctccg
     1501 gactatgtcg cacgcgggac tcttgaccct cacgtagcgt catggtctat gctgggcgca
     1561 tgaagatgga cccacacaag actcgcccgc gcgccgcggc ctcgattgca ccggcgctcc
     1621 gcgtgggcga ggtcgcccgc ctcgcgcacg tgagcgtgcg gacactgcat cactacgacc
     1681 acgtggggct gctcgagccc tccgggcaca cggcatctgg ctaccgactc tacacgctcc
     1741 ccgacctgga gcgcctccag cacgtgctgt tgtacaagga actcgggttc tcgttggagg
     1801 agatccgaga gtcactgacc gacccgtcgt tcggccggcg cgaggcgttg cagggccagc
     1861 gcgggctgct cgaggagcag atgctccgtc tggaggccct gctgggtctc atcgacaaga
     1921 cactggtatc cctggaagga gggttcggca tgaccaaaga ggagatgttc gaggtgttcg
     1981 acggcttcga cccggccgag tatgaggacg aggtcaagga gcgctgggga cacaccgagg
     2041 cctacaagga gtccgcgcgg cgcaccaagg gctacacgaa agcggattgg gagaggttca
     2101 aggcggagag cgaggagatc ggcacggcga tcgccgcgct catggacgag ggtatcccgc
     2161 ccgaggatcc ccgcgcaatg gatcaagtgg agcggcaccg cctccagatc gaccgctggt
     2221 tctacccctg ctcccacgag atgcacgtgg gcctgggcca gatgtacgtg gccgacccgc
     2281 gcttcaccgc cacctacgag aagatccacc cgggaatggc ccagtacgtc tgtgacgcca
     2341 tcatcgccaa tgccgcccgg gccgaggctg agcgccgctg accccgggga cctcgccgcc
     2401 aacgcggccc gggacggcgc ccgccctcgc tggccttctg gggccggccg ccaagccgcc
     2461 aagccggcaa gccggcgccc atgacggcgc ccggactaca ccgactcgat ctcggccgac
     2521 atcaccgcga tgtcggtgcg cagcacccag ccccgccggc gccaccagcc aacggcgccc
     2581 tcgttctcgt ggtagacgga cacgtgacac ttctggatgc cgttcgaccg cagcgaggag
     2641 acgcacgctt ccaccagcgc cgtgccgatg ccgcgcctgc gccaggtagg atcgacggcg
     2701 agatgatgga tgaagccgcg ccgcccgtcg tggccgcaca gcaccgcccc cacgaggacg
     2761 ccatcagcgc gcgcgacgaa gctggacccg ggattgcgct cgaggtaaac gaggatgttc
     2821 gcgcgctcgt ccgcgccgct caggccgatg ccctcgcacc gcttccagag tgagaggact
     2881 tcgtcgtagt ccgtgatgtc gaatggaagg atcatgtgct cacgccgtcg atcgggctct
     2941 gcatctatgg cgagtcgtcg ccgacgaact ctccgtagta cttctccgcg cattccggac
     3001 atactccgtg cgagaagacc gcttccgagt gctcgcctat gtacttctca aggacttccc
     3061 attcgtcatg cttgcggatc ttcttacaga aggaacagac aggaagcagc ccctccagca
     3121 tgacaagccg atccaacagt ctccacgtga gcccgatcga tatggccccg agtacaagca
     3181 cgaacgagga ttcaagggcg gcctcagtcc agttgatggg agttgccgga gccccgagaa
     3241 ccttgtgcgg aagatcgagc acctcgtcgc acgctatgag tgtgaccaga atcgagaagc
     3301 cgatcagctg atacgagata gcccttctcg caaggttcct cttgctactc atggtcgttc
     3361 gagcggcatg cgtcgttcga gcagcgcccg tcgttcgagc ggcatcgacc caccgagcag
     3421 catccagtgg tgtcaagaag gatgtggatc gaacccggtt caaacacgtg aacctcctct
     3481 cggggatgaa ggcgtcaccg gacgtcgaac tccaccgact tgagcaactc gtcgccgaca
     3541 tagagttcga ccctgtacgt gccgagaggc cacggcccct cgttggtgag ggtgaagaac
     3601 gtagggccgc tactggtggc ggtctcgacc tcgtcgatga tcaggcccgg gtcggcatca
     3661 tccacttcga cggcgaccca tgaggccttg aggttggtgt ccgcgggagc atcggtgagt
     3721 tccacgtgcg cgtagaagat ggcgtcctga gcgtaagtgg cgacgcgctc ggcaccctcc
     3781 tcgtcggccg acatccatgc gtcggagatc ctggccgcgc cagatccggc ttcgcacccc
     3841 gagaataccg acagcaccga gaccaccgac agcaccgaca gcagtc
//
LOCUS       DASRMJ010000359         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_180112, whole genome shotgun sequence.
ACCESSION   DASRMJ010000359 DASRMJ010000000
VERSION     DASRMJ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180112"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFD74_09925"
     CDS             complement(<1..804)
                     /locus_tag="VFD74_09925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZK49899.1"
                     /translation="MVDGIFRRVSEDIPENPFVLRLWDGTERRYGAGSPRFAISVDGP
                     ATLSEMATRGSLGTGEAYMSGALEVEGDLQSFARLAFHPAVKTLQIRPRDKAKILALY
                     LRNRNSVRKARQNIEHHYDLGNDFYQLWLDDTMTYSCAYWRDDCDDLEMAQRAKYEHI
                     CNKLHLQEGERLVDVGCGWGGMLLYAAQEYGVEGVGYTLSTEQHRFANERLKAAGLYP
                     QVRAELLDYRDAEGEFDKFVSIGMFEHVGKEYYGEFFSKVGELLAPGGVG"
     gene            complement(845..2308)
                     /locus_tag="VFD74_09930"
     CDS             complement(845..2308)
                     /locus_tag="VFD74_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HZK49900.1"
                     /translation="MTITAQTKVHELCDAYPYMRDWLAEYAADFKKLKNPVLYNTVGR
                     VTSLETAASMAGVTVERFLDDVRQAVAEHEIVAQESAGAAPRLDADERVRRQVVLKDI
                     IKELHDGASVAAVKARFDELVRDVDASEVAHMEQALIAEGLPVEEVQRLCDVHVTVFK
                     ESLDSGEETAVADDHPVAAYRRENAVIAEITSALRGALEVLVSAAAAAGDAARRAAID
                     AIAADLARLAEIDVHYLRKENQLFPVLERYGVVGPTKVMWALDDDIRSRIKVERAHAS
                     RLDAAALAASLPETLKMIDDMIYKEEKILFPTSLQVIAAGDWAAIAAGDADIGYAWIE
                     GPSGSRAREAAAAWAPAAGELLLPLTTGALSPAQVDLMLRNLPFDITYVDADDRVRYY
                     SEGERVFPRSPAAIGREVRNCHPPKSLDKVEQILAEFKAGTKDMAEFWIDLGGTFVHI
                     RYFALRDGAGAYQGCLEVVQDATHVRGLTGQRRIVDW"
     gene            2527..2910
                     /gene="gloA"
                     /locus_tag="VFD74_09935"
     CDS             2527..2910
                     /gene="gloA"
                     /locus_tag="VFD74_09935"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HZK49901.1"
                     /translation="MRIAHTMIRVTDLARSLDFYTRVLGMTLTARAEFAEGRFTLAFL
                     GFDDNPDGSAIELTHNWDTHEYELGNGFGHLAIEVDDVQAAVEQVRKRGGTVTREPRA
                     MNAGTTVIAFVADPDGYAIEFVPSR"
BASE COUNT          532 a         1063 c         1002 g          532 t
ORIGIN      
        1 tcccacccct ccaggggcca acaactctcc tactttcgag aagaactccc cgtagtactc
       61 cttacccaca tgctcgaaca tgccgatgga cacgaacttg tcgaactcgc cctcggcgtc
      121 gcggtagtcg aggagttcgg ctcgcacctg cggatagagc cccgcggcct tgaggcgctc
      181 attcgcgaac cggtgctgct cggtggagag tgtgtagccc acgccttcca cgccgtactc
      241 ctgggcggcg tacagcagca tccctcccca gccacatccc acgtccacca accgctctcc
      301 ttcttggagg tggagcttgt tgcagatgtg ctcgtatttc gcccgctggg ccatctcgag
      361 atcgtcgcaa tcgtcgcgcc agtaggcgca ggagtaggtc atggtgtcgt cgagccacaa
      421 ctggtagaag tcgttgccga ggtcatagtg gtgttcgatg ttctggcggg ccttgcgcac
      481 gctgttccga ttgcgcaggt acagggctag gatcttggcc ttgtcgcgag gtcggatctg
      541 cagggtcttc actgccgggt ggaaggctag gcgggcgaaa gactgaaggt ctccctccac
      601 ctccaaggct ccggacatgt aggcctcacc cgtgcccaga gacccacggg tggccatctc
      661 ggatagggtc gcgggcccgt cgacggagat cgcgaagcgg ggagacccgg ctccgtaccg
      721 gcgctccgtc ccgtcccaga gacgcagcac gaatggattc tcggggatgt cctcggatac
      781 cctccgaaaa atgccgtcca ccacgtctat caacgccgcc tcctaccagt cgacgatacg
      841 cttcctacca gtcgacgatg cgccgctgcc ccgtgagtcc ccgcacgtgg gtggcgtcct
      901 gcactacttc cagacagccc tggtaggccc cggcgccgtc ccgcagggcg aagtagcgga
      961 tgtgtacgaa cgtgccgccg agatcgatcc agaactcggc catgtccttc gtgcccgcct
     1021 tgaactcggc caggatctgc tccaccttat caaggctctt cggggggtga cagttgcgta
     1081 cttcgcggcc gatggccgca ggggaccgcg ggaacacgcg ctccccttca gagtagtagc
     1141 gcacgcggtc gtctgcgtcg acgtaggtga tgtcgaaggg caggttgcgg agcatcaagt
     1201 ccacttgggc cgggctcagc gcgcccgtgg tgagcggcag caggagttcg cccgcggcgg
     1261 gcgcccaggc tgccgcggct tcgcgcgcgc ggctgcccga gggcccttcg atccaggcat
     1321 agccgatgtc ggcgtcgccg gcggcgatcg cggcccagtc cccggccgcg atcacctgca
     1381 gcgaggtggg aaagaggatc ttctcttcct tgtagatcat gtcgtcgatc atcttcaggg
     1441 tctcgggcag ggaggcggcg agcgccgcgg catccaggcg cgaagcgtgg gcgcgctcca
     1501 ccttgatgcg cgagcggatg tcgtcgtcga gagcccacat caccttcgtc ggccccacca
     1561 cgccgtatcg ctcgagcacc ggaaagagct gattctcctt ccgcagatag tgaacgtcga
     1621 tctcggcgag ccgcgcgagg tcggcagcga tggcatcgat cgccgcgcgc cgagcggcat
     1681 cgccggcggc ggccgcggcg ctgaccagca cctccaatgc gccgcgcagg gcactggtga
     1741 tctcggcgat gactgcgttc tcgcgtcggt aggcggccac ggggtggtcg tcggcgacgg
     1801 ccgtctcctc gccgctgtcg agcgactcct tgaacacggt cacgtgtacg tcgcagagtc
     1861 gttgaacttc ctccaccgga aggccttcgg cgatgagcgc ctgctccatg tgggccacct
     1921 cggaggcgtc gacgtcgcgg accagttcgt cgaaccgcgc cttcaccgcg gccacggagg
     1981 cgccgtcgtg caactccttg atgatgtcct tgagaacgac ctggcggcga acccgctcgt
     2041 cggcgtcgag gcgaggcgcg gcaccggcgg actcctgcgc cacgatctcg tgctcggcga
     2101 cggcctgccg cacgtcgtcg aggaaccgtt ccaccgtcac tccggccata gaagccgctg
     2161 tctcgagcga ggtgacccga ccgaccgtgt tgtagagcac aggattctta agcttcttga
     2221 aatcggcggc gtattccgcc agccagtcgc gcatgtaggg ataggcgtcg cagagttcgt
     2281 gcaccttggt ctgagcggtg atggtcattg aacgactcct cgtcgtgttc ggcgtatcat
     2341 cgggtcaata ccccgaaggt aggtcgatga tactcgacgg cgccctgccc ccgccatatc
     2401 atcgtgccga tcgacgaggt cgaacgtgcg gaacatcgcc gagcgcgtcc ggcgggcacg
     2461 gcagggccga cgtgccggac agagccgacg tccccgaccg cagacaactc accgactgga
     2521 ggcatcatgc gcatcgccca caccatgatc cgcgtcaccg acctcgcacg ctccctcgac
     2581 ttctacacca gggtgctggg gatgacgctc actgcgcgcg cggagttcgc cgagggccgg
     2641 ttcacgttgg cgttcctggg attcgacgat aaccccgacg gctccgccat cgaactcacg
     2701 cacaactggg acacccacga gtacgaactc ggcaacggct tcggccacct ggccattgaa
     2761 gtcgacgacg ttcaggctgc cgtggagcag gtgcgcaaac gcggcggcac cgtaacccgc
     2821 gagccccgcg ccatgaacgc cggcaccacc gtgatcgcct tcgtcgccga cccggacggc
     2881 tacgccatcg agttcgtgcc gtcgcgctag gagtcggagc cgcggcagcc gagcccggac
     2941 attgatgccc ccgcggcagt cgagcccgga cgccgacacg gccggggcca ccacgaccga
     3001 gccgcagcag ccgagacccg gcaccttcac acaatcctga cacgacgctg acagtacctt
     3061 ctcacccgtg ggagatagtg tggccaggaa gaatacgagg cgccgcggaa gcggggcgcc
     3121 ttcgaggag
//
LOCUS       DASRMJ010000360         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_215042, whole genome shotgun sequence.
ACCESSION   DASRMJ010000360 DASRMJ010000000
VERSION     DASRMJ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215042"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFD74_09940"
     CDS             complement(<1..559)
                     /locus_tag="VFD74_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK49902.1"
                     /translation="MTSERQRHVARIVERALAEESLDEWEIEVLLGVDDLETAESLFG
                     AAQVLRERHFGRKVFLYGFVYFSTFCRNSCTFCLYRRGNTAGLRYRKTRDEVVAIACG
                     LAESGVHLVDLTMGEDPDIYDGGDYGVVADLVREVRLASGVPVMVSPGVVPGEVLRSL
                     RDAGADWYACYQETHTPDLYRRLRPG"
     gene            complement(556..1497)
                     /gene="pylSc"
                     /locus_tag="VFD74_09945"
     CDS             complement(556..1497)
                     /gene="pylSc"
                     /locus_tag="VFD74_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043767 - pyrrolysyl-tRNA synthetase
                     activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrrolysine--tRNA(Pyl) ligase large subunit"
                     /protein_id="HZK49903.1"
                     /translation="MERETSERAKTARTTKEAGTVGFSPTQHQRLAELGYAESGLEAL
                     FSSAGDRDARFESLSQDLTQETLARLESLRVERHRPEVRLVESELVDALVSAGFVEVV
                     TPVIIAKASLAKMGLTETHPLWRQVYWVDGDRCLRPMLAPNLYVMLSHLQRLWPSPVR
                     IFEVGPCFRKESKGANHLSEFTMLNLVELTSEPGDAERQRELAELVMGRLGLDFHLAA
                     TDSEVYGATVDVEVGGIEVASGVVGPHPLDAEWGIQGAWTGLGFGLERLAMLRTGATR
                     VHAVGRSLAYLDGARLNVQRMRPAAPKRSEADSGRTA"
     gene            complement(1517..1876)
                     /gene="pylSn"
                     /locus_tag="VFD74_09950"
     CDS             complement(1517..1876)
                     /gene="pylSn"
                     /locus_tag="VFD74_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007781556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043767 - pyrrolysyl-tRNA synthetase
                     activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrrolysine--tRNA(Pyl) ligase small subunit"
                     /protein_id="HZK49904.1"
                     /translation="MGTQATGESGGLPELPAWVFVHVGRPKKRTVRRHVDPYGIIAKV
                     KLWPSKTGLLHGVRSVKLKGGFIELETHCGQSIRMRNSRNSRVARHLRNKVFEKPCPS
                     CRIPSWKLEKFGETTFI"
     gene            complement(2060..3100)
                     /locus_tag="VFD74_09955"
     CDS             complement(2060..3100)
                     /locus_tag="VFD74_09955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtaA/CmuA family methyltransferase"
                     /protein_id="HZK49905.1"
                     /translation="MSLNMTHKERMLAALAGAPVDRVPVFSANQTATYGQMDVSGAEW
                     PEAHQKAGPMAKLAMAAHTALGFDAVRVPYCQTMESEALGCGVKYAGREGIPRIDVHA
                     YHIGDPISLPDDFLERGRIPELLEAVRILKRELGDEVAVMGNVVGPFSIVANLIGITE
                     ALKASFKKPESLLPLLEVAEQAGTVLGRALVDAGADIIVIEDMMASIDVISPPIYRDV
                     AQQPEARQVAALSDVPTIMHICGKLNPIISDVAATGVSGISVEPTVDAVAARAVLREL
                     PRHVALIGGVDAVETLYSGTPEDVKRDVRKALADGYDLIAPGCSVPPSAEMANLKAMV
                     EAAEESRAPQPA"
     gene            complement(3106..3753)
                     /locus_tag="VFD74_09960"
     CDS             complement(3106..3753)
                     /locus_tag="VFD74_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HZK49906.1"
                     /translation="MTEHEILEAATQAVVAADEDKAVEVAKEAVALGLDPLKVINQGF
                     TPGMRVVGDLFERGTMFLPEVILASEAMVAAVAILEESLPEGQREKQVGTVVLGTIEG
                     DVHDIGKGIVATLLRVYGFQVHDLGRDVALTTFVDKVKETGAGIVGSSALMTTTMVGQ
                     KALEKMLKEAGLRDQVKTMVGGAVVTQYWADKIGADAYAENAQEAVAKALELAAN"
     gene            complement(3827..>4138)
                     /locus_tag="VFD74_09965"
     CDS             complement(3827..>4138)
                     /locus_tag="VFD74_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
                     /protein_id="HZK49907.1"
                     /translation="SHALASGMGGIRTAGDLVARMQLHRKMRLPEAKAYVADKLRVST
                     RDLSDEVAMRELREELDIGVVTSVPGAARGLEAKARISDLLGVTIPSVERLKRKVGGI
                     G"
BASE COUNT          678 a         1535 c         1208 g          717 t
ORIGIN      
        1 gtccgggccg cagccgccga tagagatcgg gcgtgtgcgt ctcctggtag cacgcgtacc
       61 agtccgcacc ggcgtcccta agcgaccgca gcacttcgcc gggcacgacg cccggcgaca
      121 ccatgaccgg tacgccgctc gcgagacgca cctcacggac cagatcggcg acgaccccgt
      181 agtcgccgcc gtcgtagatg tccgggtcct cacccatggt caggtcgacc aggtgcacgc
      241 ccgactcggc cagaccgcac gcgatggcca ccacctcatc ccgtgtcttc ctgtaacgga
      301 ggccggcggt gttgcccctg cggtagagac agaacgtgca gctatttcgg cagaaggtgg
      361 agaagtacac gaacccgtac aggaagacct tccgtccgaa gtgccgctcg cgcagaacct
      421 gcgcggctcc gaacagcgac tcggcggtct ccaagtcatc gactccgagc aagacctcga
      481 tctcccactc gtccaacgac tcctcggcga gcgcccgctc gacgatccgc gccacatgcc
      541 gttgccgctc cgacgtcaag ccgtcctccc actgtcagct tccgagcgct tcggagcggc
      601 cggtctcatc ctctggacgt tgagccgcgc cccgtccagg tacgccaggc tcctgcccac
      661 ggcatgcacg cgggtcgccc cggtccgcag catggccaag cgctccagac cgaaccccag
      721 cccggtccac gcgccctgga tcccccactc agcgtcgagc ggatgaggcc ctacgacgcc
      781 ggacgctacc tcgatccctc cgacctcgac gtcgaccgtc gccccgtaca cctcggaatc
      841 ggtggccgcg aggtggaagt ccagacccag gcgacccatg accagctccg ccagctcgcg
      901 ctgacgctcc gcgtctcccg gttccgacgt gagctccacg aggttcaaca tggtgaactc
      961 cgagagatgg ttcgcgccct tcgactcctt acggaagcag ggccccactt cgaagatgcg
     1021 cacgggagaa ggccacagac gttgcaggtg gctgagcatc acgtacaagt tcggagccaa
     1081 catcggacgg agacaccgat ctccgtcgac ccagtagacc tgccgccaca gcgggtgggt
     1141 ctccgtcaga cccatcttcg ccagtgacgc cttcgcgatg atgacgggcg tgaccacctc
     1201 gacgaacccg gcggacacca gcgcgtccac cagttccgac tccaccagac gcacctccgg
     1261 ccggtgcctc tccacccgaa gcgactcgag ccgtgcgagg gtctcctgcg tcaagtcctg
     1321 gctcagcgac tcgaaccggg catcgcggtc acccgcactc gagaagagag cttccaaccc
     1381 ggactccgcg tacccgagtt ccgccagcct ctggtgttgc gtcggtgaga agccgaccgt
     1441 gccggcttcc ttcgttgtcc tcgctgtctt cgcccgctcg gacgtctccc gctccatgat
     1501 ccctctcggc cgcccctcag atgaacgtcg tctctccaaa cttctcgagc ttccacgacg
     1561 ggatgcggca gctcgggcag ggcttctcga acaccttgtt gcgcaggtga cgtgccaccc
     1621 ggctgttccg cgagttgcgc atccggatcg attgaccgca atgcgtctcg agctcgatga
     1681 agccgccctt caacttcacc gacctcaccc cgtgaagaag gccggtcttc gagggccaga
     1741 gcttcacctt cgcgatgatg ccgtatggat cgacatgcct gcgaacggtc cgtttcttcg
     1801 ggcggccgac atgcacgaag acccacgccg gcagttcggg caacccgccg ctctcacctg
     1861 tcgcctgcgt tcccatcgcg cgaccgcctt tcaccactcg gacggacgtt ggattggagg
     1921 agagtccggg agtccaaccc ggctacgaag atttagagtc tccgcggtca tgccgaccaa
     1981 ccctccccga tgccggcccg ccgaacggcc gacgtatccg ccgcgcctgc ctctgcggat
     2041 gccgcggcgc caccatgcac tacgcgggct gcggcgcgcg gctctcctcg gcggcttcga
     2101 ccatggcctt cagattcgcc atctccgccg agggcggaac cgaacagccc ggggcgatca
     2161 gatcgtagcc atcggccagc gctttgcgca cgtccctctt cacatcttcg ggggtgccgg
     2221 agtacagcgt ctccaccgcg tccaccccgc ctatgagcgc cacgtgtctc gggagttccc
     2281 gcagcaccgc tcttgccgcc accgcgtcga ccgtcggctc gacgctgatg ccgctgacac
     2341 ccgtggccgc cacgtcggaa atgatgggat tgagtttccc acagatatgc atgatcgtcg
     2401 ggacgtcaga gagggcggcc acctgccgcg cttcgggctg ttgcgcaacg tcgcggtaga
     2461 tcggggggct gatcacgtcg atggacgcca tcatgtcctc gatcacgatg atgtccgcac
     2521 cggcgtccac cagtgcccgg ccgagcaccg tgcccgcctg ctcggccacc tcgaggagcg
     2581 gcagcaggct ctcgggcttc ttgaagctgg ccttcagcgc ttccgtgatg ccgataaggt
     2641 tcgcgacgat cgagaacggc ccgacgacgt tccccatgac ggccacctcg tcacccaact
     2701 ccctcttgag tatccggacg gcctcgagca actccgggat gcggccccgc tcgaggaagt
     2761 catcggggag gctgatggga tcgccgatgt gatacgcgtg cacgtcgatg cgcgggatac
     2821 cctcccgccc ggcgtacttg acgccgcagc cgagagcctc gctctccatg gtctggcagt
     2881 aggggacgcg caccgcgtcg aagccgagcg ccgtgtgggc cgccatcgcc aacttggcca
     2941 tgggcccggc cttctggtgc gcctccggcc actcggcgcc cgagacgtcc atctgaccgt
     3001 acgtggcggt ctggttggcc gagaacaccg gcacccggtc caccggcgcg cccgccagcg
     3061 ccgccaacat ccgttccttg tgtgtcatat tgagactcac ccgtcctagt tcgcagccaa
     3121 ctccaacgcc ttcgccacag cctcctgggc gttctcggcg tatgcgtcgg cgcctatctt
     3181 gtccgcccag tactgcgtga cgacggctcc gcccaccatg gtcttcacct ggtcgcgcag
     3241 gccggcctct ttcagcatct tctccaacgc cttctgcccc accatcgtgg tggtcatcag
     3301 ggctgacgaa ccgacgatgc cggcacccgt ctctttcacc ttgtccacga aggtagtcag
     3361 agcgacgtca cgcccgaggt cgtgcacctg gaacccgtag acgcgcaaga gcgttgccac
     3421 gatacccttg ccgatatcgt gcacatcgcc ctcgatggtg cccagcacga cggtgcctac
     3481 ctgcttctcc cgctggccct cgggcagact ctcctcgagg atggcgaccg ccgccaccat
     3541 cgcctccgag gccaggatca cctccggcaa gaacatggtg cctctctcga aaaggtcgcc
     3601 cacgacgcgc atgcccggtg tgaagccttg gtttatgacc ttcagggggt cgagacccag
     3661 ggccacggct tccttggcca cctcgacggc cttgtcctcg tcggcagcca ccaccgcctg
     3721 ggtcgcagcc tcgaggatct cgtgttctgt catagccgat cggtcctttc tcgattcata
     3781 tgcccgggac cggacacgcc cggccgattc cgcgggcccg ggcgtctcag cctatgccgc
     3841 cgaccttccg cttgagccgc tccaccgaag ggatagtcac cccgagcagg tcggagatgc
     3901 gggccttggc ctccaggccg cgggccgccc ccggcaccga cgtcaccaca ccgatatcga
     3961 gttcttcgcg gagttcccgc atcgccacct cgtcggacag gtcacgcgtc gatactctca
     4021 acttgtctgc aacatacgcc tttgcctcgg ggagtcgcat cttgcggtgg agttgcatcc
     4081 tggcgaccag atctccggcc gtgcggatgc cccccatccc ggaggcgagc gcatgtga
//
LOCUS       DASRMJ010000361         4695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_180556, whole genome shotgun sequence.
ACCESSION   DASRMJ010000361 DASRMJ010000000
VERSION     DASRMJ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180556"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFD74_09970"
     CDS             complement(<1..255)
                     /locus_tag="VFD74_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49908.1"
                     /translation="MSHGANRRHALAPRNSLIDKPVGIYAGAGASHSWTWLVDCLEAQ
                     GVPRLRLLSEEDPAAGALDGLSALLIGGGDPQAMAAGMGPG"
     gene            complement(252..1553)
                     /locus_tag="VFD74_09975"
     CDS             complement(252..1553)
                     /locus_tag="VFD74_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZK49909.1"
                     /translation="MTAGLDWSRLSRRQMLQLDACTRCGECQTLCPVFEHDSREGVVP
                     RGKARGVKRLLQEESRLLRLFGRRVSEQETEALISDLYECSVCGQCREVCPVEIDTVE
                     LWESLRAAAVAAGKGPLPGHQALYQSIQNNENPWQQPRTSRDRWAMRAAKGKNAEIEP
                     VANITERPAEVLYFVGCTASFDMNLKQVAINTARVLQAAGVDFAMLGQDERCCASVMM
                     RVGLRDEFEERARANVEQLNSLGIRTLVTSCAGCFKTIGQDYPRVAPLNFEVLHISQF
                     MLRLLDEGRLALDGEVPMTVTYHDPCHLGRHRGVYDAPREVLGRVPGINLVEMERSRE
                     RARCCGAGGGLKSGFPDVQGKISHGRVQEAEATGAEALVSSCPFCYQGLQVGITLSGS
                     ALRMMDLTEVVSAALEKPAALEAEAPAGVGGSTEQDRPVTP"
     gene            complement(1550..2290)
                     /locus_tag="VFD74_09980"
     CDS             complement(1550..2290)
                     /locus_tag="VFD74_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HZK49910.1"
                     /translation="MSYHQPAFFYLLAFVAVAILAVGVGARLLLWRKLGSSGPVRLGR
                     AGLTNLLGRSFFQTRLLKLSLSRWLIHMAIFWGFLLLFAESLWLMVIKWGLPEGGSAA
                     LFFEDGGGRAVLDLWGDVWGVVLLVGLVAALVRRYVLRSAQLQTLLEDATALWFLLVV
                     TLTGFAAEGVRMALEGDQSSWAFLGGAFAWVRTLFGIDDPMTLFWVHGGLSLLLIAYL
                     PYGKLMHVLAAPVNIVLDPAASEEGEAV"
     gene            complement(2306..3529)
                     /locus_tag="VFD74_09985"
     CDS             complement(2306..3529)
                     /locus_tag="VFD74_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZK49911.1"
                     /translation="MYLYNEKILVVDLAAGSVKVEPLADDLAEESIGGAGLAAALLER
                     HTDGAPFVFSTGPLTGTLFPGGALAVLSRKQAAGELVHAPLTLFMGSELKYAGFDAVI
                     IKGVSPRPVYLWVHDGLASLEDASALAGLDTWETSDAIKRDKGDDVVQVLAVGPAGEA
                     ACTGAQVVANYWATADRAGFGGVLGSKGLKAIALRGLGLIDVEEPEDLLEHSLELGAK
                     AAASAPAHGVGGADPALREWLAPFIHRHRGCYSCALACFTYLKYNEDSSIMGSALEEP
                     GFLATDAEALEVMRAGGLSAEGALRLLEAAARRGLAPLPVARIAAERGSVDLSLLAEV
                     EGQDHTPESAAAASATAVLGYLVGVCPTALAAAPALTELAMLEALRLGSGLELQAGEF
                     AGKLPAGLSVLADGS"
     gene            complement(3546..3881)
                     /locus_tag="VFD74_09990"
     CDS             complement(3546..3881)
                     /locus_tag="VFD74_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZK49912.1"
                     /translation="MSVHLRFEPLKCIGCGRCEVACGHLRDEGYTVTSSSLMLYRAEE
                     KKNFFGLLYKTSAELVGARPDGVVVNKHGEPQEGASPAGKPILLRPPCDVCDPAPCVR
                     SCPTGAVAL"
     gene            complement(3866..>4695)
                     /locus_tag="VFD74_09995"
     CDS             complement(3866..>4695)
                     /locus_tag="VFD74_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZK49913.1"
                     /translation="AGVVAGLLEEHVLPDVPCLIAVDHSLAGGAITALAERFGPENLS
                     VVVLDAHTDAIPVPVTADAIFYDAETNPRSHYDLEDPLLHGRVDSYNASSFLHHLLES
                     GIVLPRNLHLMGVADYPPSRAFRLEDPRMVRYTGAYAALKKRGVNILTRDDIAANRAR
                     VDWELDRIDTPYLYVSVDLDVGAGSALKGVRFRDRKGLAPEILESVAASLGRAVEKVE
                     LVGLDISEIDPRTAGPGLRAPGDRTYDFACLFIEALLGNRLTATVPPSWKGETCLCT"
BASE COUNT          830 a         1638 c         1534 g          693 t
ORIGIN      
        1 gcccggcccc atgcccgccg ccatcgcctg gggatcgcct ccgccgatca ggagcgcgct
       61 gaggccgtcg agtgcgcccg ccgccgggtc ttcctcgctg aggaggcgca ggcggggaac
      121 cccctgcgcc tcgaggcaat cgaccagcca ggtccacgag tgcgaggccc cggcgccggc
      181 gtagatgccg accggcttgt cgatcagcga gttccgtggc gcgagtgcgt gtcgtctgtt
      241 cgccccgtgg ctcacggtgt gacgggccgg tcctgctcgg tcgacccgcc cacgcccgca
      301 ggggcctccg cctccagggc ggccggcttc tccagggccg cactcacgac ctcggtgagg
      361 tccatcatgc gaagtgccga gcccgacaaa gtgatgccca cctgaaggcc ctgatagcaa
      421 aaggggcagg acgagacgag cgcttcggcg ccggtcgcct cggcctcctg cacgcgaccg
      481 tggctgatct tgccctgcac gtcggggaaa ccggacttga ggcctccgcc ggccccgcag
      541 caccgggcgc gctcgcggct ccgctccatc tcgacaaggt tgatgccggg cacccgcccg
      601 agcacctcgc gcggcgcgtc gtacacgcct cggtgccggc cgaggtgaca gggatcatgg
      661 tacgtcaccg tcatcgggac ttccccgtcg agcgccagcc ttccctcgtc gagcagccgc
      721 agcatgaact gcgagatgtg aagcacttcg aagttgagcg gcgccacccg tgggtagtcc
      781 tgcccgatag tcttgaaaca gccggcgcag gatgtcacca gcgtacggat acccagcgaa
      841 ttgagctgct cgacgttggc gcgagcgcgc tcttcgaact cgtcccgaag gccgacgcgc
      901 atcatcacgc tcgcgcagca gcgctcgtct tggccgagca tggcgaagtc gacccccgcc
      961 gcctgaagga ctctcgcggt attgatcgcc acctgcttga gattcatgtc gaacgacgcg
     1021 gtgcacccga cgaagtagag cacctcagcc gggcgctcgg tgatgttggc tacgggctcg
     1081 atctcggcgt tcttgccctt ggccgcccgc atggcccaac gatcgcgcga cgtgcgcggt
     1141 tgctgccacg ggttctcgtt gttctggatg ctctgataga gcgcctgatg gcccggcaac
     1201 ggccccttgc cggcggccac ggcggccgcg cgcagactct cccacaactc cacggtatcg
     1261 atctcgaccg gacacacctc gcgacactgc ccgcacaccg agcactcgta gagatcgctg
     1321 atcagcgcct cggtctcttg ctcgctcacc ctccggccga acagacggag gagccgactc
     1381 tcctcctgca gcagccgctt gacccctcgg gccttgcccc ggggcaccac cccctcgcgg
     1441 gaatcatgct cgaacacggg acagagcgtc tggcattcgc cgcagcgggt gcaggcgtcg
     1501 agttggagca tctgccgccg actgaggcgg gaccagtcaa gaccggcggt catacggcct
     1561 caccttcttc agaagcagcc gggtccagca cgatgttcac cggagccgcg aggacatgca
     1621 tcagcttgcc gtagggcagg taggcgatca gaagaagcga caagccgccg tgcacccaga
     1681 agagggtcat gggatcgtcg atcccgaaca gcgtcctgac ccaggcgaag gcgccgccca
     1741 ggaacgccca cgacgactga tcgccttcca gagccatccg gaccccttcc gcggcgaacc
     1801 cggtgagggt caccacgaga aggaaccaaa gggcggtggc gtcttcgaga agcgtctgca
     1861 actgggcgga gcggagcacg tatcggcgca cgagggccgc caccaatccc accaacagca
     1921 cgacccccca gacgtctccc cagagatcga ggacggcccg accgccgccg tcctcgaaga
     1981 agagcgcggc ggacccccct tccggcaatc cccacttgat caccatcaac caaaggctct
     2041 cagcgaagag gagaaggaac ccccagaaga tcgccatgtg gatgagccag cgggagagac
     2101 tcaacttcaa gagccgggtc tggaagaagg agcgtcccag caggttggtg agaccggccc
     2161 ggccgagtcg gaccgggccg gaggagccta gtttcctcca cagcagaagg cgtgcaccca
     2221 cgccgacggc caggatcgcc accgccacaa aggccaacag gtagaagaag gcgggttgat
     2281 gataggacac gtgccccctt cctctctagg agccgtctgc gagcaccgac agacccgcgg
     2341 gaagcttccc tgcgaattcg ccggcctgca gctcgaggcc gctgccgagt ctcaaagcct
     2401 ccagcatagc cagttcggtg agcgcggggg cggcggcgag ggccgtgggg cagacgccga
     2461 caagatagcc cagcacagcc gtggcgctcg ccgccgccgc agactcaggt gtgtggtctt
     2521 ggccctcgac ttccgccagc agcgagagat cgaccgagcc gcgctcggca gcgatccggg
     2581 ccaccggaag aggcgcgagg ccgcggcgcg ccgcggcctc caggagccga agcgcgccct
     2641 cggcggagag cccaccagcc cgcatgactt cgagcgcttc ggcgtcggtg gccaagaagc
     2701 cgggctcttc gagggccgac cccattatgg acgagtcctc gttgtacttg aggtaggtga
     2761 agcacgccag cgcacacgag tagcaacccc ggtgacggtg gatgaaggga gcaagccact
     2821 cgcgtagcgc cggatcggcc ccgcccacgc cgtgcgcggg cgccgaggcc gccgccttcg
     2881 ctcccagttc gagggagtgc tcgaggaggt cttcgggctc ctcgacatcg atcaggccga
     2941 ggccccgcag ggcgatggcc ttcagtcctt tggagcccaa gactccgccg aaccccgccc
     3001 ggtcggcggt ggcccagtag ttcgccacga cctgcgcgcc ggtgcaggcg gcctcacccg
     3061 ccggacccac cgccaacacc tgaaccacgt cgtcgccttt gtcacgcttg atggcatccg
     3121 acgtctccca cgtgtcgagc ccggcgaggg cggaagcgtc ctcgagagaa gccagaccgt
     3181 cgtgcaccca caagtacact ggacgaggcg agacgccctt gatgatcacg gcgtcgaacc
     3241 ccgcgtactt gagctcgctc cccatgaaga gcgtgagggg ggcatgcacg agctctccgg
     3301 cggcttgctt ccgcgacagc accgccaacg cgccccccgg gaacaacgtg ccggtgagcg
     3361 ggcccgtgga gaatacgaag ggagctccat cggtgtggcg ctcgagcaac gcggccgcga
     3421 gccccgctcc cccgatgctc tcctcggcga gatcgtccgc gagcggctcg accttgacgg
     3481 atccggcggc gaggtctact accagtatct tttcgttgta gagatacatc tgtcgcttct
     3541 cgctttcaga gggccacggc gccggtcggg cacgaccgga cgcagggggc ggggtcacac
     3601 acgtcacagg gggggcgcag caagatcggc ttgcccgccg gggaagctcc ctcctggggt
     3661 tccccgtgct tgttcaccac gactccgtcg gggcgcgcgc ccaccaactc ggcagacgtc
     3721 ttgtagagta ggccgaagaa gttcttcttc tcttccgccc gatagagcat caggctggag
     3781 gaagtgaccg tgtagccctc gtcgcgcagg tgtccgcagg ctacttcaca gcggccgcaa
     3841 ccgatgcact tcagcggctc gaacctcagg tgcacagaca tgtctctcct ttccacgacg
     3901 gcggcactgt cgccgtgagt cgattgccga gcagagcctc gatgaagagg caggcgaaat
     3961 catacgtgcg gtcaccggga gcccggaggc ccggcccggc tgtgcggggg tcgatctcgc
     4021 tgatgtcgag gcccacgagc tccaccttct ccacggcccg gcccagagac gcggcgacgg
     4081 actcaaggat ctcgggggcc aggcccttgc gatcacggaa gcgcaccccc ttcagagcac
     4141 ttcccgcgcc gacgtccaga tcgacggaga cgtagaggta gggagtgtcg atgcggtcca
     4201 actcccagtc tacccgtgcc cggttcgccg cgatgtcatc gcgcgtcagg atgttcacgc
     4261 cccgcttctt gagagcggcg tacgccccgg tgtagcgcac catgcgcgga tcttccaggc
     4321 ggaacgcccg gctgggggga tagtccgcca cccccatgag gtgcaggttg cggggcagga
     4381 cgatccccga ctccagcagg tggtgcagga acgagctggc gttgtagctg tcgacccgcc
     4441 cgtggagcag gggatcctcg agatcgtagt gagaccgggg gttcgtctcc gcgtcgtaga
     4501 agatcgcgtc agccgtgacc ggcacaggga tcgcgtcggt gtgtgcgtcg agcacgacca
     4561 ccgacaggtt ctccggcccg aaccgttcgg cgagcgccgt gatcgcgccg ccggccagcg
     4621 agtggtccac ggcgatcaaa cacggcacat ctggaagcac atgctcttcc agcaggccgg
     4681 ctaccacacc ggcag
//
LOCUS       DASRMJ010000362         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_361189, whole genome shotgun sequence.
ACCESSION   DASRMJ010000362 DASRMJ010000000
VERSION     DASRMJ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361189"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..947)
                     /locus_tag="VFD74_10000"
     CDS             complement(30..947)
                     /locus_tag="VFD74_10000"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HZK49914.1"
                     /translation="MSKVKRCGLIVAGVAVVLVASAFAAAEYTGRTAFCVSCHEMQPY
                     YDSWQQFQHTEAGCADCHIPKGLVSFTKTKLFAFREVYVHVLGQVKAPLAVTRKIPDS
                     SCTQCHNSLPRAQTADATFDHTQHSAPCVRCHVRLVHKAVTPPVYVDPGTMGACFVCH
                     DGATAAKTCSLCHTAPHDDQGACDTCHGVENWSPSGFDHPFPLEGGHAKATCEQCHTK
                     TTGADGSITFSKASPECVSCHGDEHGGLTDCGRCHTPQGWTPANFSHPRVGEHIPGGE
                     HRLSCQDCHASGFGAATCTPCHDGVPRGD"
     gene            1394..>2283
                     /locus_tag="VFD74_10005"
     CDS             1394..>2283
                     /locus_tag="VFD74_10005"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZK49915.1"
                     /translation="MSSRHAGVPVVFFDTTLRDGEQSPGISLNMSEKLEIAHQLVRLG
                     VDVIEAGFPATSPGDFEAVQAIAREVKGAVVCGLARCVESDIRTCWDAIKDAEHPRIH
                     TFVSTSDIHLEHQIKKTRAEVLELTREMVTLAVSLCPGDVEFSAMDATRSAVDYLAEV
                     LQVAIECGATTINVPDTVGYTLPSEFAALIDSLYEKTPGLAGVVLSVHCHNDLGLAVA
                     NSLAAVEHGATQVEVAVNGLGERAGNAALEEIAMALVTRRDQVDRPVNIITTEIARTS
                     RMVSNLTGYQVQPNKAIVGK"
BASE COUNT          373 a          761 c          765 g          384 t
ORIGIN      
        1 ctctactcca cgattcgcga gggagtggcc tagtcgcccc gtggcacacc gtcgtggcag
       61 ggcgtgcacg tggcagcgcc gaaaccggac gcgtgacagt cctggcacga cagccggtgt
      121 tcgccaccgg gaatgtgctc cccgacccga ggatgcgaga agttcgccgg agtccatccc
      181 tggggggtgt ggcagcggcc gcagtccgtg aggccgccgt gctcgtcgcc gtggcacgag
      241 acgcactccg gcgatgcctt gctaaacgtg atcgatccat cggcgcccgt ggtcttcgtg
      301 tggcactgct cgcaggtggc tttggcgtgg ccaccctcca gcgggaaggg gtggtcgaac
      361 ccggaaggcg accagttctc cacgccatgg cacgtgtcgc aggcgccttg gtcgtcgtgg
      421 ggggcggtgt ggcacagcga gcaggtcttg gcggcggtgg caccgtcgtg gcagacgaag
      481 caggcgccca tggtgccagg gtcgacgtac acgggcgggg tcacggcctt gtggaccaga
      541 cgcacatgac atctcacaca cggtgcggag tgctgcgtgt gatcgaaggt cgcgtcggcg
      601 gtttgtgccc ggggcagact gttgtggcat tgggtgcagc tggagtcggg gatcttgcgg
      661 gtcaccgcga gcggcgcctt cacctgaccg agcacgtgca cgtagacctc acggaaggcg
      721 aagagcttgg tctttgtgaa gctgaccaat cccttgggga tgtggcagtc ggcgcacccg
      781 gcttctgtat gctgaaactg ctgccaggag tcgtagtacg gctgcatctc gtggcacgag
      841 acacaaaacg cggtgcgacc ggtgtactcg gcggcggcga aggcggaggc gacgagtacc
      901 accgcgaccc ccgcgacgat cagcccgcat ctcttcacct tcgacatggg tcgattgtct
      961 cactattccg attgtgtgcg gccgcctcgt tctaggcctt cccttgccct ggaggtggtg
     1021 cgtgtactag actcctcacc atgcggcaga ctgctcacac atcagcgagt gcccgcggcg
     1081 acgcttcccc gcgtcggccg cttggtgtcg ccctcctcct cgccctcctt cttctgctcc
     1141 cccgctaggg ggagaagtca tgcgaagccg gcgctttccg gcacgtcacc gccagcgaag
     1201 cccagaccac agcaagggga gacccatgtc cacatccgaa ccaaacgccg aggaccccac
     1261 gcgtgtagcc gccgcggccg ccgcccgggg cgccgccgaa ggcgtcccag cagacccggc
     1321 caccgcccgc gcgccggtcg gtgcgcccgc gcgccgccac gccggggccg tcgatcaagt
     1381 tgcggcgccc gggatgtcta gccgccacgc cggcgtcccg gtcgtcttct tcgataccac
     1441 cctccgcgac ggagagcagt ctccgggcat cagcctcaac atgagcgaga agcttgagat
     1501 cgcccaccag ttggtgcgct tgggcgtgga cgtgatcgaa gcgggcttcc ccgccaccag
     1561 tcccggcgac ttcgaggccg tgcaggccat cgcgagggag gtgaagggcg cggtcgtctg
     1621 cggcttggcc cgctgcgtag agagcgatat ccgcacctgt tgggatgcca tcaaggatgc
     1681 cgagcatccc cgcatccaca ccttcgtctc cacttccgac atccatctgg agcatcagat
     1741 caagaagacg cgtgcggagg tgttggagct tacgcgtgag atggtgacgc tcgcggtctc
     1801 attgtgtccg ggcgacgtgg agttctcggc catggatgcg acccgatccg ccgtcgacta
     1861 cctggccgaa gtgcttcagg tcgccatcga gtgcggcgcc acaaccatca acgtgcccga
     1921 cacggtgggc tacacgctgc cgagtgagtt cgcggcgctc atcgacagcc tctacgagaa
     1981 gacccccggc ttggccggtg tggtgctctc tgtgcactgc cacaacgacc tgggtctggc
     2041 cgtcgccaac tccctggccg ccgtggagca cggcgccacc caggtggagg tggccgtgaa
     2101 tggtctgggc gagcgggccg gcaacgccgc actggaggag atcgccatgg cgctggtcac
     2161 ccggcgggac caggtcgatc gcccggtgaa catcatcacc accgagatcg cccgcaccag
     2221 ccgcatggtc tccaacctca ccggctatca ggtgcagccg aacaaggcca tcgtgggcaa
     2281 gaa
//
LOCUS       DASRMJ010000363         4288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_286263, whole genome shotgun sequence.
ACCESSION   DASRMJ010000363 DASRMJ010000000
VERSION     DASRMJ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286263"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="dnaA"
                     /locus_tag="VFD74_10010"
     CDS             <1..1000
                     /gene="dnaA"
                     /locus_tag="VFD74_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZK49916.1"
                     /translation="ESFVIGPSNRFAHAAALAAAETPGTNYNPLFIYGGVGLGKTHLL
                     QAIGHYVLRHSPHLKVCFITIETFTNEFINSLRDETIEGFKRRYRSNDVLLIDDIQFL
                     ENKEQTQEEFFHTFNTLYEAGKQIVISSDRSPKGLVSLEDRLVSRFEWGLTTDIQPPD
                     LETRIAILRKKVLAEGVALTNEDVLTHIASRVPTNIRELEGCLTRVVAFASFNNRPID
                     VALAREVLKDIPEVAGTRVTVELILGVVSSTLGVSIAEIKGDRRTRSVVQARHVAMYL
                     ARELTDASLPKIGERMGGRDHTTVLHAVDKVGSLLKKDREIYNMIQQLTTRIKSAR"
     gene            1326..2432
                     /gene="dnaN"
                     /locus_tag="VFD74_10015"
     CDS             1326..2432
                     /gene="dnaN"
                     /locus_tag="VFD74_10015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZK49917.1"
                     /translation="MRLATRKADLVECLSIVNRAVSSRSSIQVLSGILVDCSGETPML
                     SATDMEISLKAPLAARIDDPGSLVLPARILTDIAKSLPAGEVIIEVRAGETQVEVRSG
                     ESFFSLHAMDAADFPQFPVFPAEHGFTVQKTAFLETIDRVATSASRDETRPVLTGVLI
                     HVSKSSVKMVATDSYRLSVMETPIVATVADKVQAIIPARSLVELSRIGTASSADTITI
                     IPTDNQVLFEVGGVRLSSRLIGGQFPNYRQLVPESFDYEVAVDKAELLEVVHRVGLLA
                     QKSAPVRLRFEENTLTVAAESQDLGRARESVPVKYTGEALEIGFNPEFVVAGAAAVSG
                     ETVILRFISPLRPGLIKGEADDFLYLIMPIRLTD"
     gene            2448..2690
                     /locus_tag="VFD74_10020"
     CDS             2448..2690
                     /locus_tag="VFD74_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016510622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZK49918.1"
                     /translation="MERSALAVTDGITLGQFLKFAGVVGTGGHAKVLIEDGEVRVNGE
                     VEQHRGRKLRSGDVVAALGREIAVAHRESSGPAASR"
     gene            2687..3778
                     /gene="recF"
                     /locus_tag="VFD74_10025"
     CDS             2687..3778
                     /gene="recF"
                     /locus_tag="VFD74_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HZK49919.1"
                     /translation="MIVRTVRLSGVRCHESLVVELGPGISVLVGANGAGKTSVLEGIH
                     VVLSGGSPRTTQPKELISWGQETLRAELDLLGDDGRVCTAAFGYSDSGERRCTADGAP
                     LVDTSRWEESLPVRMFLPDDVRLVRGSPRRRRRFVDRMAVTADPSYRNDLSAYDEALS
                     QRNTLLRRGIVGQDHSAWEALMARTGLKIVRARTRTLQSVSGPYASLHDLLAAPRVDS
                     PPALVYRTNASDLDETGYRERLAEQRESDRRRTFTNLGPHRDDVRFTYQNRDLRSFGS
                     QGEQRTALLALLLAERVWTAERTGSAPLLLLDDVMSELDEPRRRALIALLGEGGQVVI
                     TTTDLHHFGAEELLPMTVVPLGPPGGAAE"
     gene            3970..>4288
                     /locus_tag="VFD74_10030"
     CDS             3970..>4288
                     /locus_tag="VFD74_10030"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HZK49920.1"
                     /translation="MPGFARLGELLGNALPPPPTGVRVDSRLVAEVWLKVMGPEGAAN
                     SQPRHLRNGRLVVATQSSAWAQALQDRGDEVIARLNSHLGEAAIVSVSFRPAGWDPCA
                     GPDA"
BASE COUNT          769 a         1386 c         1395 g          738 t
ORIGIN      
        1 cgaatcattc gtcatcggcc catcgaaccg gttcgcccac gccgcagcct tggccgcggc
       61 ggagacgccc ggcaccaact acaaccctct cttcatctac gggggggtcg gcctcggcaa
      121 gacgcatctc ctgcaggcta tcggtcacta cgtgctgcgc cactctccac acctgaaggt
      181 ctgcttcatc acgatagaga ccttcaccaa cgagttcatc aactccctgc gcgacgagac
      241 gatcgagggg ttcaagcggc gctaccgcag caacgacgtg cttctgatcg acgacattca
      301 attcctcgag aacaaggaac agacgcagga ggagttcttt cacaccttca acacccttta
      361 cgaagccggc aagcaaatcg tcatatccag cgaccgctcg cccaaaggac tcgtgtctct
      421 ggaggaccgc ctggtgtcgc gtttcgagtg gggtctcacc accgatatcc aaccccccga
      481 cctggagacg cgcatagcca tactccgcaa gaaggtgctc gctgagggcg tcgcgctcac
      541 caacgaggac gtcctcactc acatcgcttc gcgggttccc acgaacatcc gtgaactcga
      601 gggctgcctc acccgggtgg tcgccttcgc ttcgttcaac aaccggccca tcgacgtcgc
      661 tttggcgcgc gaggtattga aggatattcc tgaggtggcg ggcacgcgag tcacggtgga
      721 gttgatcttg ggcgtggtct cttccacact cggggtctcg atcgcggaga taaaaggcga
      781 caggcgcacg cggtcggtcg tgcaggctcg tcacgtggcc atgtatctcg cgcgggagct
      841 caccgacgcc agccttccca agatcggcga gcgcatgggc ggtcgcgacc acaccaccgt
      901 gctgcacgcc gtggacaaag tcggtagtct tttgaagaag gatcgcgaga tctacaacat
      961 gatccagcag ttgaccaccc gcataaagag cgctcgatga gatcgggtcg gcccactgct
     1021 tgcgggtctg taacggccgc tgcgttcgtt tctcacacag tggggataca atcctgcacg
     1081 accctgtgca aagagtgggc aactcgaccc cttccggcta ggcaatgggg aagtagggtc
     1141 ggttctacac accgcctccc caccttcacg acggtgccga gtcggctcgc caccaggctg
     1201 cactcgactt gtccccggtg tccacagtgc ctataacaac aaacacctat ttagataaga
     1261 agacttgaga taatgcttgt ccacaccatc acatgcggag tcaacccggt agccgaggag
     1321 agcccatgag actagctacc cgcaaagccg atctggtcga gtgcctgagt atcgttaaca
     1381 gagcagtgtc gtcccgcagc tccatccagg tgctgtccgg gattctcgtg gactgttccg
     1441 gagaaacgcc gatgctctcg gccacggata tggagatatc gctgaaagct ccgctggccg
     1501 cccgcatcga cgacccaggt tcgctggtgt tgccggctcg gatcctcacc gacatcgcca
     1561 agagtctgcc ggccggagag gtcatcatcg aggtgcgcgc cggcgagacc caggtggagg
     1621 tacgctcggg agagtccttc ttctcccttc acgcgatgga cgccgccgac ttcccccagt
     1681 tcccggtgtt cccagccgaa cacggtttca cggtgcagaa gacggctttc ctcgagacca
     1741 tagacagggt cgccacttcc gcatcccggg acgagacgcg gccggtgctc accggggtct
     1801 tgatccacgt gtcgaagagc tcggtcaaga tggtggcgac cgacagctac cggctgagcg
     1861 tcatggagac cccgatcgta gccacggtgg ctgacaaggt tcaggccatc atcccagcgc
     1921 ggtcgttggt ggaactctcg cgcataggca cggccagttc cgcggacacg atcaccatca
     1981 tccccaccga caaccaagtg ttgttcgaag tcggcggagt gaggctcagt tcgcggctca
     2041 tcggcggcca gttccccaac taccggcagt tggtgcccga gtccttcgac tacgaggtcg
     2101 cggtggacaa ggcggagttg ctcgaagtgg ttcaccgagt cggcctcttg gctcagaaga
     2161 gcgcgcctgt gcgtctccgt ttcgaggaga acacgctcac cgtcgccgcc gagtcgcagg
     2221 atcttgggag ggcgcgtgag agcgtgccgg tgaagtacac aggcgaggct ctcgagatag
     2281 ggttcaaccc ggagtttgtc gtcgcgggag ccgcagccgt cagcggagag acggtgatcc
     2341 tgaggttcat cagcccactg cgccccggtc tgatcaaggg cgaggccgac gacttcctct
     2401 atctcatcat gcccatccgc ctaacagact gagttcggag ataccacgtg gagcggagcg
     2461 ccctagcggt gaccgatggg ataaccctcg gccagttcct caagttcgcc ggtgtagtgg
     2521 gaacgggagg gcacgccaag gtcctcatcg aggatggaga ggtgcgggtg aatggcgagg
     2581 tcgagcagca ccgaggccgc aagttgcgtt ccggtgatgt ggtggcggca ctgggccggg
     2641 agatagccgt cgcgcatcga gagtcgagcg ggccggcagc ctcacggtga tcgtcaggac
     2701 cgtccgtctt tcgggcgtcc ggtgccacga gtcgctcgtc gtcgaactgg ggccggggat
     2761 atctgtgctc gtgggtgcga atggagcagg caagaccagc gtgctcgaag gtatccacgt
     2821 ggtcctgtcg ggaggttctc cgcgaacgac ccagcccaag gagttgatct cgtggggcca
     2881 ggagacgttg cgggccgagc tcgatctgct cggcgacgac ggcagggtct gcacggccgc
     2941 cttcgggtac tcggactccg gtgaacgccg ctgcacggcg gacggtgcgc ccttggtgga
     3001 cacgtcccgt tgggaagaga gtctgcccgt gcgcatgttc ctgcccgacg acgtgcgttt
     3061 ggtgcggggg agcccgcgcc gccgccgccg gttcgtcgat cggatggcag tgacggccga
     3121 tccttcgtac cgaaacgatc tgagcgccta tgacgaagcc ctgtcgcagc gcaacacgct
     3181 cctccgtcgg ggtatcgtgg ggcaggacca ctccgcctgg gaggctctta tggccaggac
     3241 cggtttgaag atcgtgcgcg ctcgcacccg caccctgcag agcgtgtccg ggccgtacgc
     3301 gagccttcac gacctcctcg ccgctccgcg ggtcgacagc ccgccggcgc tggtgtatcg
     3361 cacgaacgcc tcggatctcg acgagaccgg ctatcgggag cgactcgccg agcagcggga
     3421 atcggaccgc cggcgcacgt tcaccaatct gggtccccac cgcgacgacg tgcggttcac
     3481 gtatcaaaac cgggatctgc gcagtttcgg atctcaaggt gaacagcgga cggccctcct
     3541 cgctctgttg ctggccgaga gagtctggac ggcagagcgc acggggagcg ctcccctact
     3601 gctgctcgac gacgtcatga gcgaactgga cgaaccgcgc cgccgggccc tcatcgcgct
     3661 cttgggcgag gggggccaag tggtgatcac gaccaccgat ctgcaccact ttggagccga
     3721 agaactccta cccatgacgg tggtgcccct gggtccgccg gggggggcgg ctgagtgagc
     3781 cggcctcagg gaccgaagcg cgacccgcgg gtcggcgggc agcctgaagt cggagcgggc
     3841 gggacggcgc ccaaagcgcc ggggccggaa gtggtgcccg gtccgccgcg cggagcggcc
     3901 gccggagccg tgcccggcgc ggcgcccgga gcaacatcag gagcgcgtcc tgccgcagcg
     3961 cccccggcgg tgccgggttt cgcccgcctc ggcgagctcc ttggcaacgc ccttccgcca
     4021 ccgccgacag gagtccgagt cgacagccgg ctcgtggccg aggtgtggct gaaggtcatg
     4081 ggccccgagg gcgccgccaa ctcccaaccg cggcatcttc gcaacggcag gctggtggtg
     4141 gcgacgcagt cgtcggcctg ggcgcaggcg ctgcaggacc gaggtgacga ggtcatcgcg
     4201 cgcctcaact cgcatctcgg ggaggccgcg atcgtgtccg tctcgttccg ccccgcgggg
     4261 tgggacccgt gcgccggccc tgatgccc
//
LOCUS       DASRMJ010000364         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_216269, whole genome shotgun sequence.
ACCESSION   DASRMJ010000364 DASRMJ010000000
VERSION     DASRMJ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216269"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFD74_10035"
     CDS             complement(<1..711)
                     /locus_tag="VFD74_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZK49921.1"
                     /translation="MTANDRTQPAHRVVVTGIGVVSALGLGREEFFAGLVAGRSAVRL
                     IEHFDTSEYAVKIAAEIPSFDPAEFMDKKAARRMDRYSQYAVAAAVMAREDAGLSLDG
                     DPYEVGTSVGSGIGGLDTFFEQTKVLHERGPDRINPFFIPMMIPNMAAAHVSMALGLK
                     GPVNATCTACAASANALGDAFQLIRRGDAPVMFAGGAEAPIGQIGIGSFAAMRALSTR
                     NDEPERASRPFDAGRDGFV"
     gene            complement(731..1477)
                     /gene="fabG"
                     /locus_tag="VFD74_10040"
     CDS             complement(731..1477)
                     /gene="fabG"
                     /locus_tag="VFD74_10040"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZK49922.1"
                     /translation="MSPLQGRVALVTGGGRGIGREIVLRLGQLGADVAVNDVARPEAA
                     EGVVAELEAMGRRGVVALGDVSNAGDVKTVVEVAEAALGPIDILVNNAGLTRDGLLMR
                     MEEADWDLVIDVNLKGAFLMSRAVARGMMKRRSGSIVNLSSVVGRRGNAGQANYSAAK
                     AGLIGLTKTLARELASRGIRVNAVAPGYIQTEMTAALPDEVKATIAGGTPLNRLGDPS
                     DVAEAVAFLAGDTAGFITGVVLPVDGGLGI"
     gene            complement(1533..2471)
                     /gene="fabD"
                     /locus_tag="VFD74_10045"
     CDS             complement(1533..2471)
                     /gene="fabD"
                     /locus_tag="VFD74_10045"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZK49923.1"
                     /translation="MDVASKRAALLFPGQGSQSVGMGRYLWEAHAHARSLFEEAEAVL
                     GWDLRGLCADGPLEDLTRTDRAQPAIFTCSVATWKVMEDRGVDFDVALGHSLGEYSAL
                     VAVGHLTFADALRVVETRGRAMWACGERQAGTMAAILGLDDEVVVRLCADAGEAWSAN
                     FNSPGQVVVSGSPGGVERVSRTAVENGARKVLPLQVSGAFHSPLVAGAAEELAEVLHR
                     AEFHPASRGRFFSTTELRYPTVAELRDVLMLQLTSPVRFTGSVETALEDVDAGIEVGP
                     GGVLAGLVKRVRRGFTVYGTDSEDTLDRAIEWALGS"
BASE COUNT          428 a          932 c          795 g          381 t
ORIGIN      
        1 gacgaacccg tcacggcccg cgtcgaaggg gcggctggcc cgctcgggct cgtcgttgcg
       61 cgtagacagg gcgcgcatgg ccgcgaacga accgatgccg atctgcccga tgggcgcttc
      121 cgccccgcct gcgaacatga cgggggcgtc accgcggcgg atgagctgga aggcgtcgcc
      181 gagcgcgttg gcgctcgcgg cgcacgcggt gcacgttgcg ttcaccggtc ccttcaagcc
      241 cagcgccatc gacacgtgag cggccgccat gttggggatc atcatgggga tgaagaaggg
      301 gttgatgcgg tcgggccccc gctcgtgcag caccttggtc tgctcgaaga aggtgtcgag
      361 cccgccgatg ccggaaccta ctgaggtccc cacctcgtag gggtcgccat ccagtgagag
      421 cccggcgtcc tcacgggcca tcacggccgc agcgacggcg tattgtgaat agcgatccat
      481 gcgacgagcg gccttcttgt ccatgaactc ggcgggatcg aaggagggga tctcggccgc
      541 tatcttcacg gcgtactcgc tagtgtcgaa atgctcgatg agtctcaccg ccgagcgacc
      601 tgcgacgagc ccggcgaaga actcctcccg gcccaagccg agcgcggaca ccacccctat
      661 gccggtgacg acaacccgat gggccggctg cgtgcggtcg ttggcggtca tgccgatccc
      721 tcccaccgcg ctagatgccg aggccgccgt cgaccgggag aacgaccccg gtgatgaacc
      781 cggctgtatc tcccgccagg aaggcgacgg cttccgcgac gtccgatggg tcgcccaggc
      841 ggttcagggg agtgcctccg gcgatggtgg ctttgacttc atcgggcaac gccgccgtca
      901 tctcggtctg gatgtatccc ggggccacgg cgttgacccg tatcccgcga gaagcgagtt
      961 cccgggccag cgtcttcgta agcccgatca gacccgcctt ggcagcggag tagttggcct
     1021 gcccggcatt cccgcgccgc ccgaccacgc tcgagaggtt gactatagac ccgctccgcc
     1081 gcttcatcat gccccgcgcc acggcccggc tcatgaggaa cgcgcccttg aggttgacgt
     1141 ctatcaccag gtcccaatcg gcctcttcca tgcgcatgag gagcccatct cgggtcaggc
     1201 cggcattgtt caccaggatg tcgataggtc ccagcgccgc ctcggccact tcgaccaccg
     1261 tcttgacgtc gcctgcgttg gagacgtccc ccaaggccac gaccccgcgg cgacccatgg
     1321 cttccagctc ggccaccacg ccctcggctg cttccggccg cgcgacgtcg ttgaccgcca
     1381 cgtcggcgcc cagttggcca aggcgcagga cgatctcgcg cccgatcccc cgtccgcctc
     1441 cggtgaccag ggccacacgg ccctgcaggg gactcatcgc tgcctcggac acgactccac
     1501 caacctttct cgctcttcga actggcccgg tctcacgatc cgagtgccca ctcgattgct
     1561 cgatccaatg tatcctcact atcggtgccg tagacggtga acccgcggcg aacgcgcttc
     1621 accaaaccgg cgagcacacc acccggaccc acctcgatcc cggcatccac atcctcaaga
     1681 gccgtctcca cggaacccgt gaaccgtacg ggcgacgtca gttggagcat gagcacgtcc
     1741 cgcagttccg ctacggtagg atacctcagc tccgtggtgg agaagaacct ccctcgcgaa
     1801 gcagggtgga actcggctcg atgaagcact tccgccaact cctccgcggc gccggccacg
     1861 agcggactgt ggaaagcgcc cgacacctgg agcggcagca ccttgcgcgc cccgttttcg
     1921 acagccgtgc gcgacacgcg ctcgacgccc ccagggctcc ccgagacgac aacctggccg
     1981 ggagagttga agttggccga ccacgcctca cccgcgtcgg cgcaaagccg cacgacgacc
     2041 tcgtcgtcca ggccgaggat cgccgccatg gtgcccgcct ggcgctcgcc gcacgcccac
     2101 atggcccggc cgcgggtctc gacgacgcga agcgcgtcgg caaaggtaag gtgacccacg
     2161 gcgaccagtg cagagtactc ccccagcgag tggcccagcg ccacgtcgaa gtcgacgccc
     2221 cgatcctcca tgaccttcca ggtggcgacg ctgcaggtga agatcgccgg ctgggcacgg
     2281 tcggtgcggg tgaggtcctc gagggggccg tcggcacaca ggccgcgcag gtcccagccc
     2341 aggacggcct ccgcctcctc gaacagcgac cgagcgtggg cgtgggcctc ccacaggtac
     2401 cgacccatgc cgaccgactg gcttccctgc ccggggaaca gcagcgcggc ccgcttcgag
     2461 gcgacgtcca tgtcagatac ctccccactc catgagacac gccccccagg taaagccggc
     2521 cccgaacccc atgaga
//
LOCUS       DASRMJ010000365         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_323325, whole genome shotgun sequence.
ACCESSION   DASRMJ010000365 DASRMJ010000000
VERSION     DASRMJ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323325"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VFD74_10050"
     CDS             <1..1130
                     /locus_tag="VFD74_10050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZK49924.1"
                     /translation="EERARPVPPPPPASQVQEEEEFYTGSVIGHLEEAPDEPEEPAAA
                     ARASAPRAAGGRPAIDEDSAIKVEAMPSVRALAKELHVDLSTIHGTGPGGRILRQDVE
                     DHAEAGVYGRPSVSAPAVPVGDVAQAEPWMGGAIEQDEHGVVERVAVRGIRRTMARRM
                     SESLAKQAQVTTTEEADITVIRRIREKERTLAAERGVRLTYLAFVIKSVAAALRRFPR
                     LNSVLQEDCEQLLLKRYYNIGIAIDTKAGLVVPNIKDADSKGIFQLAEEIVDLVERAQ
                     DRRIDLAELRGGTFTVTNYGAIGGLYATPVTNYPEISVLGMGRVRDTPVVRKGEVVVR
                     QMLPLSLTYDHQVVDGAEATRFLNLVISYLEDPDLLLLEGA"
     gene            1151..2044
                     /gene="mqnB"
                     /locus_tag="VFD74_10055"
     CDS             1151..2044
                     /gene="mqnB"
                     /locus_tag="VFD74_10055"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HZK49925.1"
                     /translation="MDETMPCRRPDARELHSLLEGAELAVIGATLAEIAPLVDRLTGC
                     ERLAVVGKQWWVGDLHSSARTIRTVVLVSGYDKVNAAHSLSCLLEAACPKLVVQTGVA
                     GAFLSSGLEVGDLAVASSEIYADLGILAPEGWLSADALAEPLAVVDGIALCNTFPLDP
                     GLVEASARIFRAGHWAGRAPKVAIGPFLTTSQVTGTSAQADLLQKRWGGIAESMEGAA
                     AAHVCALYGAPFLQVRGISNLVVDRDRGSWKVEEAARLAAQATLMVCDRLDEVLVAYR
                     AAGDDEGANDGDLDGGGPWTP"
BASE COUNT          351 a          650 c          735 g          336 t
ORIGIN      
        1 cggaggagcg agcgcgaccc gtgccacccc cgccgccggc cagtcaggtg caggaggagg
       61 aggagttcta cacgggctcg gtcatcggcc atctggaaga ggcacctgat gagcccgagg
      121 aacccgcagc ggccgcgagg gctagcgcgc cgcgcgccgc tgggggccgg ccggcgatcg
      181 acgaggattc agctatcaag gtcgaggcaa tgccctcggt gcgggcgttg gccaaggaac
      241 tccacgtgga tctcagcacc atccacggta ccggccccgg cggccgcatc ctgcggcagg
      301 atgtagagga ccacgcggag gccggggtct acggtcggcc ctcggtttcg gcgccggccg
      361 tgccagtagg ggacgtcgcg caggccgagc cctggatggg cggcgccatc gagcaggacg
      421 agcacggggt cgttgagcgc gtagcggtgc gcggcatccg ccggacgatg gcgcggcgca
      481 tgtccgaatc cctggccaag caggcccagg tgactacgac cgaagaagcc gatatcaccg
      541 tcatccgccg gatccgcgag aaggagagga ccctcgccgc cgagcgggga gtccggctga
      601 cctacctggc gttcgtgatc aagtcggtgg ctgcagcgct acgacgcttc cctcgattga
      661 actctgtgct ccaggaggac tgcgagcagc tcctgctcaa gcgctactac aacatcggca
      721 tcgccatcga caccaaggcg ggcctggtgg ttccgaacat caaggacgcc gactcgaaag
      781 gcatcttcca gctcgccgaa gagatcgtgg acctcgtgga acgcgcacag gatcggcgca
      841 tcgatcttgc cgagttgcgc ggaggcacct tcacggtgac caattacggc gccatcggcg
      901 gcttgtatgc tacaccggtc actaactatc ccgagatctc cgtccttggg atggggaggg
      961 tgcgcgacac gcccgtggtg cgcaagggcg aggtagtcgt acggcagatg ctgccgctgt
     1021 cgctgaccta cgaccaccaa gtggtcgacg gagccgaggc gacgcgcttt ctgaacttgg
     1081 tgatcagcta cctggaggat cccgacctgc tcctcctaga gggcgcctga ccgggggata
     1141 gaggcggatc gtggacgaga cgatgccctg ccgacggccg gacgcgcgtg aattgcactc
     1201 tctgctcgag ggtgcagaac tggcggtgat cggggctacc ctggctgaga tagcgcctct
     1261 ggtcgaccga ctgacgggtt gcgagcgtct ggcggtcgtg ggcaaacagt ggtgggtggg
     1321 agatctgcac tcctcagcgc ggacgatccg gaccgtggtg ctcgtgtctg gctacgacaa
     1381 ggtcaacgcg gctcactcgc tttcgtgcct tctggaagcc gcctgcccga agttggtcgt
     1441 tcagacgggg gtggcgggag ccttcctctc ctcgggactc gaggtggggg atctggccgt
     1501 ggcgtcgtca gagatctatg ccgatctggg gatcttggcg cccgagggct ggctgtcggc
     1561 cgatgccttg gcggaaccgt tggcggtggt cgacggtatc gcactgtgca acaccttccc
     1621 gctcgacccg ggtctggtcg aggcgtcggc ccgcatcttc agagcaggcc actgggccgg
     1681 acgcgcgccc aaagtggcga tcggaccctt cctgaccacg tcgcaggtga cgggtacgag
     1741 cgcgcaggcc gacctgctcc agaagcgctg gggtgggatc gcggaatcca tggagggtgc
     1801 ggccgccgcg catgtctgcg ccctctacgg cgcaccgttc cttcaggtgc gcggcatcag
     1861 caaccttgta gtcgatcggg accgtggctc gtggaaggtg gaagaagcgg cccgactcgc
     1921 ggctcaggcc acactcatgg tctgtgaccg cctcgacgaa gtgttggtcg cataccgagc
     1981 ggccggagac gacgagggcg cgaacgacgg tgacctcgat ggcggcggcc cgtggacgcc
     2041 ctgaccctag cctactcacc gtgtcccaac ga
//
LOCUS       DASRMJ010000366         4918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_324489, whole genome shotgun sequence.
ACCESSION   DASRMJ010000366 DASRMJ010000000
VERSION     DASRMJ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324489"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..736
                     /gene="raiA"
                     /locus_tag="VFD74_10060"
     CDS             170..736
                     /gene="raiA"
                     /locus_tag="VFD74_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZK49926.1"
                     /translation="MNLTVKGRNIAVTDALNAYAGEKIGRLRKYLADDARCEVELSAE
                     KNPSISDNQVAEATIRTKGPIIRGREASSDMYASIDLVADKLERQVKKYRGKVIARSQ
                     GAGIEAFLGEGLSFGEDIEAEDDHGPLPTIVKTKQFMVKPMTPEEAALQMDLVGHDFF
                     VFMSSETQETAVVYRRRDGDYGLIEPQR"
     gene            769..3381
                     /gene="secA"
                     /locus_tag="VFD74_10065"
     CDS             769..3381
                     /gene="secA"
                     /locus_tag="VFD74_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZK49927.1"
                     /translation="MGFFDKVLRFGEGKKMKSFQAGVAQVSALEDSVRPLDDAALRAK
                     TVEFRARIAQGTSIEELVPEAFAVAREMAWRTVAMRPFDVQVLGGLVLHSGAISEMKT
                     GEGKTLVATMPVYLNALGGQPVHVVTVNDYLASRDAAWMGPVYEALGLTVGVLQNNMS
                     PADRKAAYQCDVVYGTNTEFGFDYLRDNMALSLEQRVQRGHAYCIIDEVDSILVDEAR
                     TPLIISGPGEHAAKTYYDFARIASQLREGEGHDYEVDEKKRTVAPTEEGVARVEKMAG
                     VDNLYEDLSGQLVNHLMQSLRAQALFKRDVDYLVQDGEVKIIDEFTGRILEGRRYSEG
                     LHQAIEAKEGVKIKEENQTLATITLQNYFRMYEKISGMTGTAVTEADEFREIYGLEVL
                     EIPTNQPMVREDRNDLIYKTRTAKVKAVAEDVLECYEQGQPVLVGTISVEASEALSAM
                     LTRKGVPHEVLNAKHHAREATIVERAGQAGSVTIATNMAGRGTDIKLGEGVVELGGLY
                     ILGTERHDSRRIDNQLRGRSGRQGDPGASRFYISLQDDLMRLFAGDRVHSLMDRLGLD
                     DDTPIEAGMISRSVESAQKRVEEQNFQIRKRVLEYDDVMNRQREVIYAQRKRILEGED
                     LQDDVQQIIERVVRAQVDLFTSGSKYPEEWDLPELFAVLRSMFPITFDVADLGDIEEL
                     DAEELADRVLEDALETYQAKEVEVGEENMRELERWVLLRTLDSRWRDHLYEMDYLREG
                     IGLRALAQKDPLVEYKSEGFTMFQSMMDAIQEDFVRLAFRVEVRREEAPDTSRHVELS
                     YSAPDPSAAGGSFVGLTAPGGAAAADGSAGAFRQAQRAGQTVTAPRHVEKVGRNDPCP
                     CGSGKKYKKCHGAE"
     gene            3436..4606
                     /gene="prfB"
                     /locus_tag="VFD74_10070"
     CDS             join(3436..3531,3533..4606)
                     /gene="prfB"
                     /locus_tag="VFD74_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537001.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZK49928.1"
                     /translation="MSEKTQIQAAAAADYQERLPSFREKLAWVRDYFDPAGLEERVAS
                     LEGEMHAPGFWDDQAAAQKLSTQYSRVKSRLEQYSGLAELIDDLEMLAELAVEEDGEG
                     PLSPGNEAELERLSSQIERLLDAFEEARLFNGEFDAGDAIMSIHPGAGGTESQDWAEM
                     LLRMYLRWAQGRSFEVEMNEVTEGDEAGIKSATVTVHGENAYGLLAAERGVHRLVRMS
                     PFDAANRRHTSFASLDVTPVVDEAVAVDIDEKDLRVETYRASGAGGQHVNKTDSAVRI
                     THVPSKTVVQCQNERSQIQNRETAMRMLRGKLLLLQAEKREQDLAREKGVQKEIGWGS
                     QIRSYVMAPYTLVKDHRTGLEKGNVDGVLNGSIDEFIHEYLMRRATGGISEDDER"
     gene            4788..>4918
                     /locus_tag="VFD74_10075"
     CDS             4788..>4918
                     /locus_tag="VFD74_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK49929.1"
                     /translation="MIRLEEVTKIYPPDTIGLDSVSLHIEKGEFVFLVGASGSGKST"
BASE COUNT          969 a         1501 c         1667 g          781 t
ORIGIN      
        1 gactgatgcg gagtggatat ccagtctgcg gcaccgcctc acggtgggca tggtgggccc
       61 cggctgcggc ggcccggaag gcggcggtgt ggtaatgtac aggattcgat cgctccccgt
      121 ggggggcggt gtcgggtcaa accgtgggta ggctaaggag taggacagcg tgaaccttac
      181 ggtcaaagga cgcaatattg ctgttactga cgctctcaac gcatatgccg gtgagaagat
      241 agggcgattg cggaagtacc tcgcggacga cgctcgttgc gaggtggaac tctccgcaga
      301 gaagaacccg agcatatccg acaaccaggt ggccgaggcc acgatccgca ccaaggggcc
      361 catcatccgc ggccgggagg cttcgagcga catgtacgcc tccatcgatt tggtggcgga
      421 caagctcgag cgccaagtga agaagtaccg gggaaaggtt atcgcccgct cccaaggcgc
      481 cggaatcgaa gcgttcctgg gtgaagggct ctccttcggc gaagacattg aagccgagga
      541 cgatcacggt cccttgccga cgatcgtcaa gaccaagcag ttcatggtga agcccatgac
      601 ccccgaggag gcggctctcc agatggatct ggtggggcac gacttcttcg tgttcatgag
      661 cagcgaaacg caagagacgg ctgtggtcta ccggcgccgc gacggcgact acggactgat
      721 cgagccgcaa cggtagtctc gttctggggc accgggtcag gctgaaatat ggggttcttt
      781 gacaaggtcc ttcgctttgg cgagggcaag aagatgaagt cgttccaggc gggcgtcgcc
      841 caggtgtctg cgctggaaga ctctgtgcgc ccgctcgacg atgccgcgct gcgggccaag
      901 accgtcgagt tcagggcgcg catcgcccag ggcacatcca tcgaggagct ggttccggaa
      961 gcgttcgccg ttgcccgcga gatggcttgg cgcacagtgg ccatgcgccc gttcgacgtg
     1021 caggttctgg gcgggctcgt gcttcacagc ggcgccatct ccgagatgaa gaccggcgag
     1081 ggcaagaccc tcgtggccac catgcctgtt tacctcaacg ccttgggtgg tcaaccggtg
     1141 cacgtggtca cggtcaacga ctacttggca agccgcgacg ccgcctggat gggtcccgtg
     1201 tacgaggcgc tgggtctgac ggtgggcgtg ctgcagaaca acatgtctcc cgcggaccgc
     1261 aaggcggcgt accagtgcga cgtggtctac ggcaccaaca ccgagttcgg gttcgactac
     1321 ctgcgcgaca acatggcgct gtcgctcgag cagcgcgtac aacgcggtca cgcctactgc
     1381 atcatcgacg aggtggactc catcttggtc gatgaagcgc gcaccccgct tatcatctcg
     1441 ggcccggggg agcacgcggc caagacgtac tacgacttcg cgcgcatcgc gagtcagctg
     1501 agggagggcg aaggccacga ctacgaggtc gacgagaaga agcgcacggt ggcccccacc
     1561 gaagagggcg tggcccgcgt cgagaagatg gcaggcgtgg acaacctcta cgaggacctt
     1621 tccggccagc tggtgaacca cctgatgcag tcgcttcggg cgcaggccct cttcaagcgt
     1681 gacgtcgact acctcgtgca agacggcgaa gtcaagatca tcgacgagtt caccggtcgc
     1741 atcctggagg ggcgccgtta ctccgaaggc ctccaccagg ccatcgaagc caaagagggc
     1801 gtcaagatca aggaagagaa ccagacgctc gccaccatca cgctgcagaa ctacttccgc
     1861 atgtacgaga agatctcggg tatgaccggg acggccgtca ccgaggccga cgagttccgc
     1921 gagatctacg gcctcgaagt gctcgagatc cccaccaacc agcccatggt gcgcgaggac
     1981 cgcaacgacc tcatctacaa gacccgcacg gccaaggtga aggccgtcgc tgaggacgtg
     2041 ctggaatgct acgagcaggg ccagcccgtg ctcgtgggca ccatctccgt cgaggcttcg
     2101 gaggccctct cggcgatgct cacacggaag ggtgtcccgc acgaggtgtt gaacgccaag
     2161 catcacgccc gggaagcgac gatagtcgag cgggccggcc aagccggcag cgtcaccatc
     2221 gccaccaaca tggcggggcg cggcaccgac atcaagctgg gtgagggtgt cgtggaactc
     2281 ggggggctct acatcctggg caccgaacgg cacgactccc gacgcatcga caaccagctg
     2341 cgcggccgct cggggcggca gggcgacccc ggggccagcc gtttctacat ctccctacag
     2401 gacgatctca tgcgtctctt cgcgggcgat cgcgtccaca gcctcatgga ccggctcgga
     2461 ctcgacgacg acactcccat cgaggcgggg atgatcagcc ggtcagtgga gagcgcgcag
     2521 aagcgggtgg aggagcagaa cttccagatc aggaagcggg tgctcgagta cgacgacgtg
     2581 atgaaccggc aacgtgaggt catctacgcc cagcgcaagc gcatcttgga gggcgaggat
     2641 ctccaggacg acgtccagca gatcatcgag cgcgtcgtgc gcgcacaggt ggatctcttc
     2701 acctccggga gcaagtaccc cgaggagtgg gacctgccgg agctgttcgc cgtgctccgt
     2761 tcgatgttcc ccatcacctt cgacgtcgcc gacctcggag acatcgagga actcgatgcg
     2821 gaggaactcg ccgaccgggt gctcgaagac gctctcgaga cctaccaggc caaggaagtc
     2881 gaggtgggcg aggagaacat gcgggagctt gagcgctggg tgctgctccg cacgctcgac
     2941 tcgcgctggc gcgatcatct ctacgagatg gactacttgc gggagggcat cgggctgcgt
     3001 gctctggctc agaaggaccc cctggtcgag tacaagtccg agggattcac catgttccag
     3061 tcgatgatgg acgccatcca ggaggatttc gtccgtctcg ccttccgcgt ggaggtgagg
     3121 cgcgaagagg cgcccgacac atcgcggcac gtggagctct cctacagtgc tcccgaccct
     3181 tcggcggccg gcgggagttt cgtcggcctg acggctccag gcggcgcggc tgcggccgat
     3241 ggctccgccg gcgccttccg gcaggctcag cgggccgggc agacggtgac ggctccccgg
     3301 cacgtggaga aggtgggacg caacgacccc tgcccctgcg gcagcgggaa gaagtacaag
     3361 aaatgtcacg gggcggagtg agagtgtccc acgaggccga ccccctcgtc ggcgctgggc
     3421 tatacttcca cacgtatgtc tgagaagaca cagatccagg cggcggccgc cgccgactac
     3481 caggaacggc ttccgtcttt ccgcgagaag ctcgcgtggg taagggatta cctttgaccc
     3541 cgctggactc gaggagcgcg tagcctccct cgaaggggag atgcacgcgc cggggttctg
     3601 ggacgaccaa gcggcggccc agaagctctc cacccaatac agcagagtga agagccgtct
     3661 ggagcagtat tccggcctcg cggagttgat cgacgatctt gagatgctcg ccgagctggc
     3721 tgtggaagag gacggcgaag gccctctctc ccccggcaac gaggcggaac tcgagcgtct
     3781 ctcgagccag atcgagcgtc tgttggacgc attcgaggag gctcggctct tcaacggtga
     3841 gttcgacgcg ggtgacgcca tcatgagcat ccatcccggc gccggcggca ccgagagcca
     3901 ggattgggcg gagatgctcc tgcgcatgta tctgcgctgg gcccagggcc gttccttcga
     3961 ggtggagatg aacgaagtca ccgagggaga cgaagcaggc atcaagagcg ccacggtcac
     4021 cgtgcacggc gagaacgcct acggccttct cgccgcggaa cgcggagtgc accgcctcgt
     4081 gcggatgtcc ccgttcgacg ccgccaaccg gcgccacacc tcgttcgcct cgctcgacgt
     4141 gacccccgtc gtggacgagg cggtggccgt ggacatcgac gagaaggacc ttcgggtgga
     4201 gacgtaccgc gcatcgggtg ccggcggcca gcacgtgaac aagaccgatt cggccgtccg
     4261 catcacgcac gtccccagca agaccgtggt gcagtgccag aacgagcgct cgcagatcca
     4321 gaaccgcgag accgcgatgc gcatgctgcg tggcaagctg cttctgctcc aggcggagaa
     4381 acgcgagcag gatctggcca gggagaaggg tgtgcagaag gagatcggct ggggcagcca
     4441 gatccggtca tacgtcatgg ccccctacac gctggtcaag gaccatcgta cggggcttga
     4501 gaagggcaac gtggatggtg tcctgaacgg ctccatcgac gagttcatcc acgaatacct
     4561 gatgcgtcga gccaccggcg gcatcagcga agacgatgaa cgatagtgcc ggcgggcgcg
     4621 gagcgcacgt cggccggggc gtggcgtcgg cgccctcagg gtcgcgacgt cggcggcccg
     4681 ccaaggataa tggcagcgca gtcgagaact gtatccggtg cggcttcggg tcacgggtcc
     4741 gatgacgacc caccgcgaag tgcacgtcag gaggtaggca ggagaacgtg atacgtctag
     4801 aggaagtcac aaagatctat ccgcccgaca cgatcgggtt ggattcggtg agtctccaca
     4861 tcgagaaggg ggagttcgtc ttcttggtgg gtgcttccgg ttccggtaag tccacctt
//
LOCUS       DASRMJ010000367         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U10212
            k141_287824, whole genome shotgun sequence.
ACCESSION   DASRMJ010000367 DASRMJ010000000
VERSION     DASRMJ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963739
            Sequence Read Archive: SRR5665811
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:32:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,019
            CDSs (total)                      :: 1,986
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 6 of 28
            Pseudo Genes (incomplete)         :: 20 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287824"
                     /isolate="SMAG_U10212"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFD74_10080"
     CDS             complement(<1..209)
                     /locus_tag="VFD74_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK49930.1"
                     /translation="MGLSDRIGSRPADQAERAPEAQAAADSEARARSNAAYGRQEELK
                     TRIHGAVIGELGNQLYRTDVPDDDLR"
     gene            complement(244..1626)
                     /locus_tag="VFD74_10085"
     CDS             complement(244..1626)
                     /locus_tag="VFD74_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK49931.1"
                     /translation="MPDGTATPPAPSVNEAAPKRIIEVLIADDHELVRSGIRHALERT
                     GTMRVSAEAATGSEAISILALAQDYVRDSFSRALDTYPGGAVTFACADSDAALEHAER
                     ERPQLVIVSADLEPLDGYATVQEINARVPGVSTLIVAANPSPNDFRRALQAGARDMLQ
                     LPVEKKDLFAALGAAVQVSGAKRSALEGITAKATAIKEKPIARRVVVFSTKGGTGKTF
                     LSTNLAAGLAATGKRVALVDLDLQFGDVSIALGIVPQRTIYDLVQGYTDFDLALLRDF
                     MLPHPASGMFVLPAPLYPEQGDEITDSDVTRLLEAIQEDYDFVIVDTPPFFEERVLAA
                     LDWADDILLVGSLDLATLKNLKLSLASMELMSYPVEKIRIVMNRADSKVGLELADVER
                     HLGRAVRYSISSSIEVPRALNTGEVLAVQRPNLRVSQELKAVIDDYAGSVNGAAPRRF
                     GFRRKPERTS"
     gene            complement(1626..2675)
                     /locus_tag="VFD74_10090"
     CDS             complement(1626..2675)
                     /locus_tag="VFD74_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZK49932.1"
                     /translation="MAAKNPAQDGAPNLFAASRVKRGLVRAPIPALMLLGTALLYLLL
                     ADTEPAYFLFFLPVAFAGVGLGARAGVLAAVAAIAVVIVRSLLVERAYLTGELDVATI
                     DMSPYILWATFLVVTGYIVGLISQRGGSRGVGIDLGTEIMRAVEREHMRIGHDLHDTV
                     AQNAAGSLMEAEILSSMLDEASPDVRGQAQRVTNSLNACIDELRGAITNLRPPALAQP
                     EFLDSLRRLVEAFTTRTGKKVEFSVEGHPERHSDSVRICVYRVIQEALGNIEHHAQAS
                     RVYVSVRASRRTVYLLVADDGVGFDPQPYDGDESNGHFGLKGMQERVALLGGELEIET
                     APRTGTGVRARIPAL"
     gene            2831..>3274
                     /locus_tag="VFD74_10095"
     CDS             2831..>3274
                     /locus_tag="VFD74_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZK49933.1"
                     /translation="MNDALGLRDGAREALPIVLGYVPIGFAYGVLGITAGLPIWAIVA
                     MSVVVYAGSAQFMAVSLLSQGASAATLVVTTFLVNLRHLLYSSALSPRLERMSRGRLA
                     WLAAELTDESFVIAARAANARGGRLSFGFVAGLNGTAQLSWVAGST"
BASE COUNT          490 a         1083 c         1111 g          590 t
ORIGIN      
        1 cgcaggtcgt cgtcggggac gtccgtgcgg tagagctgat tgcccagttc cccgatgacc
       61 gccccgtgga tgcgggtctt gagctcctct tggcggccgt aggccgcgtt cgagcgggcc
      121 ctcgcctcgg aatcggctgc cgcctgagcc tccggtgcgc gctccgcttg gtccgcaggg
      181 cgactaccta tgcggtcgct caggcccatc tccgacctcc gctatctccg ttgtgttcgc
      241 tcatcagctc gtacgctccg gtttgcggcg aaagccgaat ctacgcggag cggctccgtt
      301 gacgctgccc gcgtagtcgt ctatcacggc cttcaactcc tgcgacactc ggaggttcgg
      361 cctctgcacc gcgagcacct cgccggtgtt cagggctctc gggacctcga tcgaactcga
      421 gatcgagtat ctgaccgcgc gccccaagtg acgctcgacg tcggccaact cgaggcccac
      481 cttggagtcg gcccggttca tgacgatgcg gatcttctcc accggatacg acatcagctc
      541 catgctggcc aagctgagct tcaggttctt gagagtggcc aggtcgaggc tgcctaccag
      601 caggatgtcg tcggcccagt cgagggcggc cagcacccgc tcttcgaaga agggcggcgt
      661 gtcgactatg acgaagtcgt agtcctcctg gatcgcctcc aacagccggg tgacgtcgct
      721 gtcggtgatc tcgtcaccct gctcggggta gagaggcgcc ggcagcacga acatgcccga
      781 agccgggtgg gggagcatga agtccctcag cagggccaaa tcgaagtcgg tgtagccctg
      841 caccaggtcg tagatggtgc gctgcgggac tatccccagg gcgatggaca cgtcgccgaa
      901 ctgcaggtcg aggtccacca gcgccacccg tttgcccgtt gcggccagac ccgccgccag
      961 gttcgtggag aggaaggtct tgccggtgcc gcccttggtg ctgaacacca ccacccgccg
     1021 cgcgatcggc ttctccttga tggccgtcgc tttggcggtg atgccctcga gcgcgctccg
     1081 cttcgccccc gacacctgca cggccgctcc cagcgcggcg aacaggtcct tcttctccac
     1141 cggcagctgc agcatgtcgc gggccccggc ctgcaacgcc cgcctaaagt cgttgggaga
     1201 ggggttggcg gccacgatca gcgtgctcac gcccgggacg cgggcgttga tctcctgcac
     1261 ggtggcgtag ccgtcgagcg gttccaggtc ggcgctgacg atcaccaact ggggacgttc
     1321 cctctccgcg tgctcgagcg cggcgtccga gtcggcgcag gcgaacgtca ccgccccccc
     1381 ggggtaggtg tcgagcgccc gcgagaagct gtctctcacg tagtcttggg ccagggcgag
     1441 gatagagatg gcctcgctgc ccgtggcggc ctcggccgag acgcgcatgg tgcccgtgcg
     1501 ctccagagcg tggcggatgc ccgaacggac cagctcgtgg tcgtcggcga tcaggacctc
     1561 gatgatccgt ttgggtgcgg cctcgttcac ggagggtgcc ggcggcgtgg cggtgccgtc
     1621 gggcatcaca gcgccgggat ccgtgcccgg acgccggtgc cggtgcgggg ggccgtctcg
     1681 atctcgagtt cccctcccag aagggcgacc cgctcctgca tgcccttcaa tccgaagtgc
     1741 ccattggatt cgtcaccgtc gtacggttgg ggatcgaagc cgacgccgtc gtcggccacc
     1801 agcaggtaga ccgtcctcct gctcgctctg acgctcacgt acacgcgcga ggcctgcgcg
     1861 tggtgttcga tgttgcccaa cgcctcctgg atgacccggt agacgcagat gcgcacggag
     1921 tccgagtgcc tctcggggtg tccttccacc gagaactcca ccttcttgcc ggtgcgcgtg
     1981 gtgaaggcct ccacgagcct gcggagcgaa tcgaggaact cgggctgggc gagggcggga
     2041 ggccgcaggt tggttatcgc tccacggagc tcgtcgatgc aggcgttcag cgagttcgtc
     2101 acacgctggg cctggccgcg cacgtcgggg ctcgcctcgt cgagcatgga ggagaggatc
     2161 tccgcctcca tgagggagcc cgccgcgttc tgcgcgacgg tgtcgtggag gtcgtgaccg
     2221 atgcgcatgt gctcgcgctc cacggcccgc atgatctccg tgccgaggtc tatccccaca
     2281 ccccggcttc ccccgcgttg ggagatcaat ccgacgatgt atccggtgac gacgaggaac
     2341 gtggcccaga ggatgtacgg actcatgtcg atggtggcca cgtccaactc gccggtgaga
     2401 taagctcgtt cgaccagcaa cgaacggact atgacgaccg ctatcgcggc taccgcggcc
     2461 agaacgcccg cccgggcgcc gagcccgacc cccgcgaagg ccacgggcag gaagaagagg
     2521 aagtaggcag gttcagtgtc cgcaagaagc aagtagagca gggcggtgcc gagaagcata
     2581 agagcgggta tgggcgcacg gacaagacct ctcttgaccc tgctcgcggc gaagaggttc
     2641 ggcgcgccgt cttgtgcggg gttcttggct gccaacccgt atccctttcg agcggcggtg
     2701 tcccgttccg aaagatgccc ggctgccacg gacccgatcc gctgctcccg cggttctgcc
     2761 ccagcggagt ctacatccgg aagggcgtcg atgatagtac atacagtaca agaacggcta
     2821 gttctccaag atgaatgacg cgctcggcct gcgtgacggc gcccgcgagg cgctgcccat
     2881 cgtgcttggc tacgtgccca tagggttcgc ctacggggtg ctgggcatca ccgccggcct
     2941 tccgatatgg gcgatcgtgg ccatgtcggt ggtcgtgtat gcgggctcgg cgcagttcat
     3001 ggcggtgtcg ctgctctcgc agggcgcctc ggcggccacg ttggtggtca ccaccttctt
     3061 ggtcaacctt cggcacctgc tctactcgtc ggcgctgtcg ccgcgtctgg agcggatgag
     3121 ccggggtcgg ttggcctggt tggcggccga actcaccgat gagagcttcg tgatcgccgc
     3181 ccgggccgcc aacgcccgtg gagggcggct gtcgttcggc ttcgtggccg gtctcaacgg
     3241 gacggcccag ttgtcgtggg tggcgggatc gacg
//
